FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Maity, TK Rho, HS Belinka, N Xie, Z Zhang, X Venugopalam, A Biswas, R Cultraro, C Zhu, H Guha, U AF Maity, Tapan K. Rho, Hee-Sool Belinka, Nataiya Xie, Zhi Zhang, Xu Venugopalam, Abhilash Biswas, Romi Cultraro, Constance Zhu, Heng Guha, Udayan TI Identification of substrates of lung cancer-specific mutant EGFR kinases. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Maity, Tapan K.; Belinka, Nataiya; Zhang, Xu; Venugopalam, Abhilash; Biswas, Romi; Cultraro, Constance; Guha, Udayan] NIH, Natl Canc Inst, Med Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Rho, Hee-Sool; Zhu, Heng] Johns Hopkins Univ, Pharmacol & Mol Sci, Baltimore, MD USA. [Xie, Zhi] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5260 DI 10.1158/1538-7445.AM2013-5260 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605307 ER PT J AU Marino, N Collins, JW Shen, CY Sledge, GW Steeg, PS AF Marino, Natascia Collins, Joshua W. Shen, Changyu Sledge, George W. Steeg, Patricia S. TI Analysis of gene expression patterns downstream of multiple metastatic suppressor genes SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Marino, Natascia; Collins, Joshua W.; Steeg, Patricia S.] NCI, Lab Mol Pharmacol, CCR, NIH, Bethesda, MD 20892 USA. [Shen, Changyu; Sledge, George W.] Indiana Univ Simon Canc Ctr, Indianapolis, IN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3871 DI 10.1158/1538-7445.AM2013-3871 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603105 ER PT J AU Marrero, AM Lawrence, SM Wilsker, D Balasubramanian, P Kinders, RJ Parchment, RE Tomaszewski, JE Doroshow, JH AF Marrero, Allison M. Lawrence, Scott M. Wilsker, Deborah Balasubramanian, Priya Kinders, Robert J. Parchment, Ralph E. Tomaszewski, Joseph E. Doroshow, James H. TI Development of a multiplex quantitative immunofluorescence assay to evaluate DNA damage repair deficient models in vitro and in vivo and the response to cytotoxic agents. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Marrero, Allison M.; Lawrence, Scott M.; Wilsker, Deborah; Balasubramanian, Priya; Kinders, Robert J.; Parchment, Ralph E.] Frederick Natl Labs, SAIC Frederick Inc, Appl Dev Directorate, Lab Human Toxicol & Pharmacol, Frederick, MD USA. [Tomaszewski, Joseph E.; Doroshow, James H.] NCI, Div Canc Treatment & Diag, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3341 DI 10.1158/1538-7445.AM2013-3341 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602048 ER PT J AU Mathews, LA Guha, R Shinn, P Young, RM Lim, KH Keller, J Liu, DB Yasgar, A McKnight, C Boxer, MB Duveau, DY Jiang, JK Michael, S Mott, BT Patel, PR Leister, W Maloney, DJ LeClair, CA Rai, G Jadhav, A Peyser, BD Austin, CP Martin, S Simeonov, A Ferrer, M Staudt, L Thomas, CJ AF Mathews, Lesley A. Guha, Rajarshi Shinn, Paul Young, Ryan M. Lim, Kian-Huat Keller, Jonathan Liu, Dongbo Yasgar, Adam McKnight, Crystal Boxer, Matthew B. Duveau, Damien Y. Jiang, Jian-Kang Michael, Sam Mott, Bryan T. Patel, Paresma R. Leister, William Maloney, David J. LeClair, Christopher A. Rai, Ganesha Jadhav, Alit Peyser, Brian D. Austin, Christopher P. Martin, Scott Simeonov, Anton Ferrer, Marc Staudt, Louis Thomas, Craig J. TI High-throughput combination screening identifies novel drug-drug pairings for a Bruton's tyrosine kinase inhibitor against the ABC subtype of diffuse large B-cell lymphomas SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mathews, Lesley A.; Guha, Rajarshi; Shinn, Paul; Keller, Jonathan; Liu, Dongbo; Yasgar, Adam; McKnight, Crystal; Boxer, Matthew B.; Duveau, Damien Y.; Jiang, Jian-Kang; Michael, Sam; Mott, Bryan T.; Leister, William; Maloney, David J.; LeClair, Christopher A.; Rai, Ganesha; Jadhav, Alit; Austin, Christopher P.; Martin, Scott; Simeonov, Anton; Ferrer, Marc; Thomas, Craig J.] NIH, Div Preclin Innovat, Natl Ctr Adv Transnat Sci, Rockville, MD USA. [Young, Ryan M.; Lim, Kian-Huat; Staudt, Louis] NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Patel, Paresma R.] NIH, SAIC Frederick, Frederick Natl Lab Canc Res, Frederick, MD USA. [Peyser, Brian D.] NCI, Informat Technol Branch, Dev Therapeut Program, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4543 DI 10.1158/1538-7445.AM2013-4543 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604148 ER PT J AU Mattoo, AR Pastan, I Fitzgerald, D AF Mattoo, Abid R. Pastan, Ira Fitzgerald, David TI The cytotoxic activity of the anti-mesothelin immunotoxin SS1P is enhanced by the PKC inhibitor enzastaurin SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mattoo, Abid R.; Fitzgerald, David] NCI, Biotherapy Sect, LMB, NIH, Bethesda, MD 20892 USA. [Pastan, Ira] NCI, Mol Biol Sect, LMB, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5490 DI 10.1158/1538-7445.AM2013-5490 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605369 ER PT J AU Mazor, R Vassall, AN Eberle, JA Beers, R Hu, XB Benhar, I Pastan, I AF Mazor, Ronit Vassall, Aaron N. Eberle, Jaime A. Beers, Richard Hu, Xiaobo Benhar, Ital Pastan, Ira TI Identification and elimination of T-cell epitopes in recombinant immunotoxins used to treat cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mazor, Ronit; Vassall, Aaron N.; Eberle, Jaime A.; Beers, Richard; Hu, Xiaobo; Pastan, Ira] NCI, NIH, Bethesda, MD 20892 USA. [Benhar, Ital] Tel Aviv Univ, IL-69978 Tel Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4334 DI 10.1158/1538-7445.AM2013-4334 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603341 ER PT J AU McGlynn, KA Divine, GW Sahasrabuddhe, VV Engel, LS VanSlooten, A Wells, K Yood, MU Alford, SH AF McGlynn, Katherine A. Divine, George W. Sahasrabuddhe, Vikrant V. Engel, Lawrence S. VanSlooten, Ashley Wells, Karen Yood, Marianne Ulcickas Alford, Sharon Hensley TI Statin use and risk of primary liver cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [McGlynn, Katherine A.; Sahasrabuddhe, Vikrant V.] Natl Canc Inst, Bethesda, MD USA. [Divine, George W.; VanSlooten, Ashley; Wells, Karen; Alford, Sharon Hensley] Henry Ford Hlth Syst, Detroit, MI USA. [Engel, Lawrence S.] Univ N Carolina, Chapel Hill, NC USA. [Yood, Marianne Ulcickas] Boston Univ, Boston, MA 02215 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4829 DI 10.1158/1538-7445.AM2013-4829 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600361 ER PT J AU Mendoza-Villanueva, DY Kim, S Ali, HR Sharan, S Sarkar, TR Caldas, C Landberg, G Sterneck, E AF Mendoza-Villanueva, Daniel Y. Kim, Suryun Ali, H. Raza Sharan, Shikha Sarkar, Tapasree R. Caldas, Carlos Landberg, Goeran Sterneck, Esta TI CEBPD (C/EBP delta) acts as a tumor suppressor in hormone receptor positive breast cancer cells and may serve as biomarker to predict the need for adjuvant therapy SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mendoza-Villanueva, Daniel Y.; Kim, Suryun; Sharan, Shikha; Sarkar, Tapasree R.; Sterneck, Esta] NCI, Frederick, MD 21701 USA. [Ali, H. Raza; Caldas, Carlos] Univ Cambridge, Cambridge, England. [Landberg, Goeran] Univ Manchester, Manchester, Lancs, England. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3464 DI 10.1158/1538-7445.AM2013-3464 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602170 ER PT J AU Miermont, AM Pflicke, H Guan, H Chinnasamy, H Thomas, C Rudloff, U AF Miermont, Anne M. Pflicke, Holger Guan, Hannah Chinnasamy, Harshini Thomas, Craig Rudloff, Udo TI Direct inhibition of ERK1/2 by VTX-11e leads to increased induction of apoptosis in a subset of pancreatic cancer cell lines as compared to MEK1/2 inhibition by selumetinib (AZD6244) SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Miermont, Anne M.; Pflicke, Holger; Guan, Hannah; Chinnasamy, Harshini; Rudloff, Udo] NCI, CCR, Bethesda, MD USA. [Thomas, Craig] NCATS, Rockville, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5538 DI 10.1158/1538-7445.AM2013-5538 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220606017 ER PT J AU Ming, M Soltani, K Shea, CR Li, XL He, YY AF Ming, Mei Soltani, Keyoumars Shea, Christopher R. Li, Xiaoling He, Yu-ying TI Keratinocyte-specific deletion in mice reveals gene dose-dependent function of SIRT1 in tumorigenesis. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Ming, Mei; Soltani, Keyoumars; Shea, Christopher R.; He, Yu-ying] Univ Chicago, Chicago, IL 60637 USA. [Li, Xiaoling] NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 1068 DI 10.1158/1538-7445.AM2013-1068 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600081 ER PT J AU Miranda, TB Voss, TC Sung, MH Baek, S John, S Hawkins, M Grontved, L Schiltz, RL Hager, GL AF Miranda, Tina B. Voss, Ty C. Sung, Myong-Hee Baek, Songjoon John, Sam Hawkins, Mary Grontved, Lars Schiltz, R. Louis Hager, Gordon L. TI Steroid receptor reprogramming of the chromatin landscape: crosstalk at the genomic level. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Miranda, Tina B.; Voss, Ty C.; Sung, Myong-Hee; Baek, Songjoon; John, Sam; Hawkins, Mary; Grontved, Lars; Schiltz, R. Louis; Hager, Gordon L.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 1075 DI 10.1158/1538-7445.AM2013-1075 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600088 ER PT J AU Modali, SD Desai, SS Parekh, VI Lonser, RR Kebebew, E Emmert-Buck, M Agarwal, SK AF Modali, Sita D. Desai, Shruti S. Parekh, Vaishali I. Lonser, Russel R. Kebebew, Electron Emmert-Buck, Michael Agarwal, Sunita K. TI Reduced expression of the long non-coding RNA MEG3 in sporadic and MEN1-associated tumors SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Modali, Sita D.; Desai, Shruti S.; Parekh, Vaishali I.; Agarwal, Sunita K.] NIDDK, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. [Lonser, Russel R.] NINDS, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA. [Kebebew, Electron] NCI, Endocrine Oncol Branch, NIH, Bethesda, MD 20892 USA. [Emmert-Buck, Michael] NCI, Adv Technol Ctr, NIH, Gaithersburg, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB249 DI 10.1158/1538-7445.AM2013-LB-249 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601178 ER PT J AU Mohammed, A Brewer, M Ritchie, RL Marya, A Lightfoot, S Janakiram, NB Steele, VE Rao, CV AF Mohammed, Altaf Brewer, Misty Ritchie, Rebekah L. Marya, Anuj Lightfoot, Stan Janakiram, Naveena B. Steele, Vernon E. Rao, Chinthalapally V. TI Metformin prevents progression of pancreatic intraepithelial neoplasia to ductal adenocarcinoma by targeting cancer stem cells and mTOR signaling SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mohammed, Altaf; Brewer, Misty; Ritchie, Rebekah L.; Marya, Anuj; Lightfoot, Stan; Janakiram, Naveena B.; Rao, Chinthalapally V.] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. [Steele, Vernon E.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2268 DI 10.1158/1538-7445.AM2013-2268 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600137 ER PT J AU Moiola, CP De Luca, P Zalazar, F Cotignola, J Labanca, E Meiss, R Vazquez, ES Gardner, K De Siervi, A AF Moiola, Cristian P. De Luca, Paola Zalazar, Florencia Cotignola, Javier Labanca, Estefania Meiss, Roberto Vazquez, Elba S. Gardner, Kevin De Siervi, Adriana TI Molecular link that associates high fat diet and prostate tumor growth SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Moiola, Cristian P.; De Luca, Paola; Zalazar, Florencia; Cotignola, Javier; Labanca, Estefania; Vazquez, Elba S.; De Siervi, Adriana] Univ Buenos Aires, Buenos Aires, Argentina. [Meiss, Roberto] Natl Acad Med, Fac Med, Buenos Aires, Argentina. [Gardner, Kevin] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3697 DI 10.1158/1538-7445.AM2013-3697 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602333 ER PT J AU Mondul, AM Moore, SC Sampson, JN Weinstein, SJ Karoly, ED Virtamo, J Albanes, D AF Mondul, Alison M. Moore, Steven C. Sampson, Joshua N. Weinstein, Stephanie J. Karoly, Edward D. Virtamo, Jarmo Albanes, Demetrius TI Metabolomic profile of response to beta-carotene supplementation reveals potential for pharmacologic interactions with beta-carotene in the Alpha-Tocopherol, Beta-Carotene Cancer Prevention (AT BC) Study. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Mondul, Alison M.; Moore, Steven C.; Sampson, Joshua N.; Weinstein, Stephanie J.; Albanes, Demetrius] NCI, DCEG, Rockville, MD USA. [Karoly, Edward D.] Metabolon Inc, Durham, NC USA. [Virtamo, Jarmo] Natl Inst Hlth & Welf, Helsinki, Finland. RI Albanes, Demetrius/B-9749-2015; OI Moore, Steven/0000-0002-8169-1661 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB30 DI 10.1158/1538-7445.AM2013-LB-30 PG 2 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601224 ER PT J AU Monks, A Rapisarda, A Wrzewczynski, KO August, EM Polley, EC Kondapaka, SB Kaur, G Newton, D Teicher, BA AF Monks, Anne Rapisarda, Annemarie Wrzewczynski, Kazimierz O. August, E. Michael Polley, Eric C. Kondapaka, Sudhir B. Kaur, Gurmeet Newton, Dianne Teicher, Beverley A. TI Target and drug discovery for recalcitrant, rare and neglected cancers. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Monks, Anne; Rapisarda, Annemarie; August, E. Michael; Newton, Dianne] Frederick Natl Lab Canc Res, Frederick, MD USA. [Wrzewczynski, Kazimierz O.; Polley, Eric C.; Teicher, Beverley A.] NCI, Rockville, MD USA. [Kondapaka, Sudhir B.; Kaur, Gurmeet] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4361 DI 10.1158/1538-7445.AM2013-4361 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603368 ER PT J AU Moody, TW Jensen, RT AF Moody, Terry W. Jensen, Robert T. TI SR48692 inhibits EGF receptor transactivation and proliferation of lung cancer cells. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Moody, Terry W.] NCI, CCR, Bethesda, MD 20892 USA. [Jensen, Robert T.] NIDDK, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 1306 DI 10.1158/1538-7445.AM2013-1306 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600091 ER PT J AU Moore, HM Engei, K Greytak, S Bass, BP Vaught, J AF Moore, Helen M. Engei, Kelly Greytak, Sarah Bass, B. Paige Vaught, Jim TI Translating biospecimen science research results to improved biospecimen practices. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Moore, Helen M.; Vaught, Jim] NCI, Bethesda, MD 20892 USA. [Engei, Kelly] Preferred Staffing Grp, Bethesda, MD USA. [Greytak, Sarah; Bass, B. Paige] Kelly Govt Solut, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5149 DI 10.1158/1538-7445.AM2013-5149 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605245 ER PT J AU Morton, LM Wong, JR Gallie, BL Abramson, DH Seddon, JM Dean, M Gold, B Goldstein, AM Kleinerman, RA Tucker, MA AF Morton, Lindsay M. Wong, Jeannette R. Gallie, Brenda L. Abramson, David H. Seddon, Johanna M. Dean, Michael Gold, Bert Goldstein, Alisa M. Kleinerman, Ruth A. Tucker, Margaret A. TI Specific RB1 mutations and risk of subsequent neoplasms among survivors of hereditary retinoblastoma. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Morton, Lindsay M.; Wong, Jeannette R.; Goldstein, Alisa M.; Kleinerman, Ruth A.; Tucker, Margaret A.] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Rockville, MD USA. [Gallie, Brenda L.] Hosp Sick Children, Dept Ophthalmol & Vis Sci, Toronto, ON M5G 1X8, Canada. [Abramson, David H.] Mem Sloan Kettering Canc Ctr, Ophthalm Oncol Serv, New York, NY 10021 USA. [Seddon, Johanna M.] Tufts Univ New England Med Ctr, Ophthalm Epidemiol & Genet Serv, Boston, MA USA. [Dean, Michael; Gold, Bert] NCI, Lab Expt Immunol, Canc & Inflammat Program, Ctr Canc Res,NIH,DHHS, Frederick, MD 21701 USA. RI Tucker, Margaret/B-4297-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 1333 DI 10.1158/1538-7445.AM2013-1333 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600118 ER PT J AU Murthy, SR Lee, TK Cawley, NX Hewitt, SM Pacak, K Loh, PY AF Murthy, Saravana R. Lee, Terence K. Cawley, Niamh X. Hewitt, Stephen M. Pacak, Karel Loh, Peng Y. TI An N-terminal truncated carboxypeptidase E splice isoform induces metastasis by activating nedd9, ceacam5, cbr1 and other metastasis inducing genes SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Murthy, Saravana R.; Cawley, Niamh X.; Pacak, Karel; Loh, Peng Y.] NICHD, NIH, Bethesda, MD USA. [Lee, Terence K.] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China. [Hewitt, Stephen M.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3877 DI 10.1158/1538-7445.AM2013-3877 PG 2 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603111 ER PT J AU Nagy, Z Orosz, E Kovacs, I Torok, M Toth, D Biro, T Blumberg, PM Czifra, G AF Nagy, Zsuzsanna Orosz, Edina Kovacs, Ilona Toeroek, Miklos Toth, Dezsz Biro, Tamas Blumberg, Peter M. Czifra, Gabriella TI Investigation of RasGRP3 expression and function in human breast cancers and breast-derived ductal adenocarcinoma cell lines. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Nagy, Zsuzsanna; Orosz, Edina; Biro, Tamas; Czifra, Gabriella] Univ Debrecen, H-4012 Debrecen, Hungary. [Kovacs, Ilona; Toeroek, Miklos; Toth, Dezsz] Gyula Kenezy Hosp, Debrecen, Hungary. [Blumberg, Peter M.] NCI, NIH, Lab Canc Biol & Genet, Seattle, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4399 DI 10.1158/1538-7445.AM2013-4399 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604005 ER PT J AU Neta, GI Rajaraman, P de Gonzalez, AB Doody, M Alexander, B Simon, S Freedman, DM Linet, M Sigurdson, A AF Neta, Gila I. Rajaraman, Preetha de Gonzalez, Amy Berrington Doody, Michele Alexander, Bruce Simon, Steve Freedman, D. Michal Linet, Martha Sigurdson, Alice TI A prospective study of medical diagnostic x-rays and risk of thyroid cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Neta, Gila I.; Rajaraman, Preetha; de Gonzalez, Amy Berrington; Doody, Michele; Simon, Steve; Freedman, D. Michal; Linet, Martha; Sigurdson, Alice] NCI, Rockville, MD USA. [Alexander, Bruce] Univ Minnesota, Minneapolis, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2541 DI 10.1158/1538-7445.AM2013-2541 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600179 ER PT J AU Nguyen, AB Moser, R Chou, WY AF Nguyen, Anh B. Moser, Richard Chou, Wen-Yong TI An examination of socioeconomic status, race, and health outcomes in the 2009 CHIS adult population SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Nguyen, Anh B.; Moser, Richard; Chou, Wen-Yong] NCI, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2513 DI 10.1158/1538-7445.AM2013-2513 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600152 ER PT J AU Nicastro, HL Lubet, RA Dunn, BK Grubbs, CJ AF Nicastro, Holly L. Lubet, Ronald A. Dunn, Barbara K. Grubbs, Clinton J. TI Genistein and soy isoflavone isolate decreased tumor incidence and multiplicity in a Sprague-Dawley ER-positive breast cancer model SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Nicastro, Holly L.; Lubet, Ronald A.; Dunn, Barbara K.] NCI, Rockville, MD USA. [Grubbs, Clinton J.] Univ Alabama Birmingham, Birmingham, AL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4873 DI 10.1158/1538-7445.AM2013-4873 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604378 ER PT J AU Nogueira, L Cross, A Freedman, N Lai, G Castro, F Koshiol, J AF Nogueira, Leticia Cross, Amanda Freedman, Neal Lai, Gabriel Castro, Felipe Koshiol, Jill TI Gallstones, cholecystectomy, and risk of digestive system cancers SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Nogueira, Leticia; Cross, Amanda; Freedman, Neal; Lai, Gabriel; Castro, Felipe; Koshiol, Jill] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4804 DI 10.1158/1538-7445.AM2013-4804 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600337 ER PT J AU Obadina, MA AF Obadina, Mofiyinfoluwa A. TI Finding correlations between CtBP expression and breast cancer risk factors SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Obadina, Mofiyinfoluwa A.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 145 DI 10.1158/1538-7445.AM2013-145 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600061 ER PT J AU Ozhand, A Mckean-Cowdin, R Bernstein, L Ballard-Babash, R McTiernan, A Baumgartner, KB AF Ozhand, Ali Mckean-Cowdin, Roberta Bernstein, Leslie Ballard-Babash, Rachel McTiernan, Anne Baumgartner, Kathy B. TI Short term reduction in mammographic density predicts survival in breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Ozhand, Ali; Mckean-Cowdin, Roberta] USC Dept Prevent Med, Los Angeles, CA USA. [Bernstein, Leslie] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA. [Bernstein, Leslie] City Hope Natl Med Ctr, Duarte, CA 91010 USA. [Ballard-Babash, Rachel] NCI, Bethesda, MD 20892 USA. [McTiernan, Anne] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Baumgartner, Kathy B.] Univ New Mexico, Ctr Canc, Albuquerque, NM 87131 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2286 DI 10.1158/1538-7445.AM2013-2286 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600145 ER PT J AU Park, KS Giuseppe, G Wang, YS Pham, T Moon, Y Luo, J Lee, LC Mitsudomi, T Okamoto, I Yatabe, Y Mok, T Raffeld, M Xi, LQ Salomon, DS Bianco, C AF Park, Kang-Seo Giuseppe, Giaccone Wang, Yisong Trung Pham Moon, Yongwha Luo, Ji Lee, Liam Changwoo Mitsudomi, Tetsuya Okamoto, Isamu Yatabe, Yasushi Mok, Tony Raffeld, Mark Xi, Liqiang Salomon, David S. Bianco, Caterina TI Cripto-1 elicits innate resistance to EGFR inhibitors in non-small cell lung cancer harboring EGFR-sensitizing mutations. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Park, Kang-Seo; Giuseppe, Giaccone; Wang, Yisong; Trung Pham; Moon, Yongwha; Luo, Ji; Lee, Liam Changwoo; Raffeld, Mark; Xi, Liqiang] NCI, Bethesda, MD 20892 USA. [Mitsudomi, Tetsuya; Okamoto, Isamu] Kinki Univ, Fac Med, Osakasayama, Japan. [Yatabe, Yasushi] Aichi Canc Ctr Hosp, Nagoya, Aichi 464, Japan. [Mok, Tony] Chinese Univ Hong Kong, Shatin, Hong Kong, Peoples R China. [Salomon, David S.; Bianco, Caterina] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4468 DI 10.1158/1538-7445.AM2013-4468 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604073 ER PT J AU Patlolla, JMR Kopelovich, L Qian, L Biddick, L Zhang, YT Desai, D Amin, S Lightfoot, S Rao, CV AF Patlolla, Jagan Mohan Reddy Kopelovich, Levy Qian, Li Biddick, Laura Zhang, Yuting Desai, Dhimant Amin, Shantu Lightfoot, Stan Rao, Chinthalapally V. TI Licofelone inhibits NNK-induced lung adenocarcinoma formation in A/J mice by suppressing COX/LOX and inhibits human lung cancer cell growth by p21 up-regulation. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Patlolla, Jagan Mohan Reddy; Qian, Li; Biddick, Laura; Zhang, Yuting; Rao, Chinthalapally V.] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. [Kopelovich, Levy] NCI, Bethesda, MD 20892 USA. [Desai, Dhimant; Amin, Shantu] Penn State Univ, Milton S Hershey Med Ctr, Hershey, PA 17033 USA. [Lightfoot, Stan] Univ Oklahoma, Hlth Sci Ctr, Dept Pathol, Oklahoma City, OK USA. [Lightfoot, Stan] VA Hosp, Oklahoma City, OK USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB182 DI 10.1158/1538-7445.AM2013-LB-182 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601115 ER PT J AU Paul, RK Wnorowski, A Gonzalez-Mariscal, I Moaddel, R Indig, FE Wainer, IW Bernier, M AF Paul, Rajib K. Wnorowski, Artur Gonzalez-Mariscal, Isabel Moaddel, Ruin Indig, Fred E. Wainer, Irving W. Bernier, Michel TI (R,R ')-4 '-methoxy-1-naphthylfenoterol Inhibits GPR55 signaling and the modulation of motility in human cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Paul, Rajib K.; Wnorowski, Artur; Gonzalez-Mariscal, Isabel; Moaddel, Ruin; Indig, Fred E.; Wainer, Irving W.; Bernier, Michel] NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5516 DI 10.1158/1538-7445.AM2013-5516 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605395 ER PT J AU Paul, RK Ramamoorthy, A Zhang, YQ Becker, KG Paris, A Cloix, JF Bernier, M Wainer, IW AF Paul, Rajib K. Ramamoorthy, Anuradha Zhang, Yongqing Becker, Kevin G. Paris, Arnaud Cloix, Jean-Francois Bernier, Michel Wainer, Irving W. TI Antitumor activity of (R,R ')-4 '-methoxy-1-naphthylfenoterol in a rat C6 glioma xenograft model in the mouse SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Paul, Rajib K.; Ramamoorthy, Anuradha; Zhang, Yongqing; Becker, Kevin G.; Bernier, Michel; Wainer, Irving W.] NIH, Baltimore, MD USA. [Paris, Arnaud; Cloix, Jean-Francois] Univ Orleans, Orleans, France. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5514 DI 10.1158/1538-7445.AM2013-5514 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605393 ER PT J AU Peskoe, SB Nelson, WG Joshu, CE Rohrmann, S McGlynn, KA Platz, EA AF Peskoe, Sarah B. Nelson, William G. Joshu, Corinne E. Rohrmann, Sabine McGlynn, Katherine A. Platz, Elizabeth A. TI Are circulating testosterone and PSA levels associated in a nationally representative sample of men without a diagnosis of prostate cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Peskoe, Sarah B.; Nelson, William G.; Joshu, Corinne E.; Platz, Elizabeth A.] Johns Hopkins Univ, Baltimore, MD USA. [Rohrmann, Sabine] Univ Zurich, Zurich, Switzerland. [McGlynn, Katherine A.] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3614 DI 10.1158/1538-7445.AM2013-3614 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600234 ER PT J AU Pfister, TD Dieckman, LJ Kinders, RJ Book, A Colantonio, S Mutreja, K Lawrence, SM Aziz, A Hiltke, T Whiteley, G Parchment, RE Tomaszewski, JE Weinberg, RA Doroshow, JH AF Pfister, Thomas D. Dieckman, Lynda J. Kinders, Robert J. Book, Anne Colantonio, Simona Mutreja, Karun Lawrence, Scott M. Aziz, Amina Hiltke, Tara Whiteley, Gordon Parchment, Ralph E. Tomaszewski, Joseph E. Weinberg, Robert A. Doroshow, James H. TI Development of recombinant transcription factor proteins and antibodies for application in clinical immunoassays SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Pfister, Thomas D.; Kinders, Robert J.; Book, Anne; Mutreja, Karun; Lawrence, Scott M.; Parchment, Ralph E.] SAIC Frederick, Lab Human Toxicol & Pharmacol, Appl Dev Res Directorate, Frederick Natl Labs, Frederick, MD USA. [Dieckman, Lynda J.; Aziz, Amina] Argonne Natl Labs, Biosci Div, Argonne, IL USA. [Colantonio, Simona; Whiteley, Gordon] SAIC Frederick, Prot Chem Lab, Frederick Natl Labs, Frederick, MD USA. [Hiltke, Tara] Natl Canc Inst, Bethesda, MD USA. [Tomaszewski, Joseph E.; Doroshow, James H.] Natl Canc Inst, Div Canc Treatment & Diag, Bethesda, MD USA. [Tomaszewski, Joseph E.; Doroshow, James H.] Natl Canc Inst, Ctr Canc Res, Bethesda, MD USA. [Weinberg, Robert A.] Whitehead Inst, Ludwig Ctr Mol Oncol, Dept Biol, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5544 DI 10.1158/1538-7445.AM2013-5544 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220606023 ER PT J AU Poletto, M Dorjsuren, D Lirussi, L Vascotto, C Scognamiqlio, PL Marasco, D Jadhav, A Maloney, DJ Simeonov, A Wilson, DM Tell, G AF Poletto, Mattia Dorjsuren, Dorjbal Lirussi, Lisa Vascotto, Carlo Scognamiqlio, Pasqualina L. Marasco, Daniela Jadhav, Ajit Maloney, David J. Simeonov, Anton Wilson, David M. Tell, Gianluca TI Modulating the APE1/NPM1 interaction in cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Poletto, Mattia; Lirussi, Lisa; Vascotto, Carlo; Tell, Gianluca] Univ Udine, Dept Med & Biol Sci, I-33100 Udine, Italy. [Dorjsuren, Dorjbal; Jadhav, Ajit; Maloney, David J.; Simeonov, Anton] Natl Ctr Adv Translat Sci, NIH Chem Genom Ctr, Bethesda, MD USA. [Scognamiqlio, Pasqualina L.; Marasco, Daniela] Univ Naples Federico II, Dept Biol Sci, Naples, Italy. [Wilson, David M.] NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. RI Marasco, Daniela/H-4848-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3337 DI 10.1158/1538-7445.AM2013-3337 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602044 ER PT J AU Pommier, YG Murai, J AF Pommier, Yves G. Murai, Junko TI Differential potentiation of temozolomide and camptothecin by the PARP inhibitors olaparib and veliparib in relationship with their PARP-DNA trapping abilities, and lack of impact of PARP inhibitors on cisplatin activity. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Pommier, Yves G.; Murai, Junko] NCI CCR, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3421 DI 10.1158/1538-7445.AM2013-3421 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602127 ER PT J AU Prickett, TD Hill, V Gartner, J Zerlanko, B Jiang, JJ Samaan, M Wunderlich, J Gutkind, S Rosenberg, SA Samuels, Y AF Prickett, Todd D. Hill, Victoria Gartner, Jared Zerlanko, Brad Jiang, Jiji Samaan, May Wunderlich, John Gutkind, Silvio Rosenberg, Steven A. Samuels, Yardena TI Somatic mutation of the NMDAR subunit GRIN2A in malignant melanoma results in loss of tumor suppressor activity SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Prickett, Todd D.; Hill, Victoria; Gartner, Jared; Zerlanko, Brad; Samuels, Yardena] NHGRI, NIH, Bethesda, MD 20892 USA. [Jiang, Jiji] Univ Maryland, College Pk, MD 20742 USA. [Samaan, May; Gutkind, Silvio] NIDCR, NIH, Bethesda, MD USA. [Wunderlich, John; Rosenberg, Steven A.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB48 DI 10.1158/1538-7445.AM2013-LB-48 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601298 ER PT J AU Raafat, AM Bargo, S Callahan, R AF Raafat, Ahmed M. Bargo, Sharon Callahan, Robert TI Activation of NF-KB by Notch4/Int3 is Rbpj-independant SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Raafat, Ahmed M.; Bargo, Sharon; Callahan, Robert] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5180 DI 10.1158/1538-7445.AM2013-5180 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605275 ER PT J AU Rangel, MC Raafat, A Castro, NP Klauzinska, M Karasawa, H Baker, A Callahan, R Salomon, DS AF Rangel, Maria Cristina Raafat, Ahmed Castro, Nadia P. Klauzinska, Malgorzata Karasawa, Hideaki Baker, Alyson Callahan, Robert Salomon, David S. TI Mammary tumorigenesis in haploinsufficient Cripto-1 mice SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Rangel, Maria Cristina; Castro, Nadia P.; Klauzinska, Malgorzata; Karasawa, Hideaki; Baker, Alyson; Salomon, David S.] NCI, Frederick, MD 21701 USA. [Raafat, Ahmed; Callahan, Robert] NCI, Bethesda, MD 20892 USA. RI Rangel, Maria Cristina/P-7216-2014 OI Rangel, Maria Cristina/0000-0002-8002-9617 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB261 DI 10.1158/1538-7445.AM2013-LB-261 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601188 ER PT J AU Ravillah, D Qian, L Brewer, M Zhang, YT Biddick, L Madka, V Patlolla, JMR Mohammed, A Steele, V Rao, CV AF Ravillah, Durgadevi Qian, Li Brewer, Misty Zhang, Yuting Biddick, Laura Madka, Venkateshwar Patlolla, Jagan M. R. Mohammed, Altaf Steele, Vernon Rao, Chintalapally V. TI Targeting HADC-2: Chemopreventive effect of OSU-HDAC42 on rat colon carcinogenesis and APCmin/ plus Mice intestinal tumorigenesis SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Ravillah, Durgadevi; Qian, Li; Brewer, Misty; Zhang, Yuting; Biddick, Laura; Madka, Venkateshwar; Patlolla, Jagan M. R.; Mohammed, Altaf; Rao, Chintalapally V.] Ctr Canc Prevent & Drug Dev, Dept Med, Hem Onc Sect, Oklahoma City, OK USA. [Steele, Vernon] NCI, Canc Prevent Div, ChemoPrevent Agent Dev Res Grp, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4392 DI 10.1158/1538-7445.M2013-4392 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603398 ER PT J AU Reed, LT Palmieri, D Mason, G Steeg, P AF Reed, L. Tiffany Palmieri, Diane Mason, Gunny Steeg, Patricia TI Characterization of the neuroinflammatory response in a mouse model of brain metastatic inflammatory breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Reed, L. Tiffany; Palmieri, Diane; Steeg, Patricia] NCI, Bethesda, MD 20892 USA. [Mason, Gunny] Inflammatory Breast Canc Res Fdn, Bainbridge Isl, WA USA. RI Palmieri, Diane/B-4258-2015 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4976 DI 10.1158/1538-7445.AM2013-4976 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605072 ER PT J AU Rivera, AV Sanchez, VC Poirier, MC Olivero, OA AF Rivera, Andrea V. Sanchez, Vanesa C. Poirier, Miriam C. Olivero, Ofelia A. TI Zidovudine (AZT)-induced aneuploidy, mediated by Stathmin 1 (STMN1), involves a loss of polymerized beta-tubulin SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Rivera, Andrea V.; Sanchez, Vanesa C.; Poirier, Miriam C.; Olivero, Ofelia A.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3603 DI 10.1158/1538-7445.AM2013-3603 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602286 ER PT J AU Rotunno, M Sun, XZ Figueroa, J Sherman, M Garcia-Closas, M Meltzer, P Williams, T Schneider, SS Jerry, J Yang, R Troester, M AF Rotunno, Melissa Sun, Xuezheng Figueroa, Jonine Sherman, Mark Garcia-Closas, Montserrat Meltzer, Paul Williams, Tyisha Schneider, Sallie Smith Jerry, Joseph Yang, Rose Troester, Melissa TI Parity-related molecular signatures and breast cancer heterogeneity. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Rotunno, Melissa; Figueroa, Jonine; Sherman, Mark; Meltzer, Paul; Yang, Rose] NCI, NIH, HHS, Bethesda, MD 20892 USA. [Sun, Xuezheng; Williams, Tyisha; Schneider, Sallie Smith; Jerry, Joseph; Troester, Melissa] Univ N Carolina, Chapel Hill, NC USA. [Garcia-Closas, Montserrat] Inst Canc Res, London SW3 6JB, England. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3651 DI 10.1158/1538-7445.AM2013-3651 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600271 ER PT J AU Rudd, ML Mohamed, H Price, JC Urick, ME O'Hara, AJ Le Gallo, M Cruz, P Zhang, SY Mullikin, J Merino, MJ Bell, DW AF Rudd, Meghan L. Mohamed, Hassan Price, Jessica C. Urick, Mary Ellen O'Hara, Andrea J. Le Gallo, Matthieu Cruz, Pedro Zhang, Suiyuan Mullikin, James Merino, Maria J. Bell, Daphne W. CA NISC Comparative Sequencing Progra TI Mutational analysis of the tyrosine kinome in serous and clear cell endometrial cancers SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Rudd, Meghan L.; Mohamed, Hassan; Price, Jessica C.; Urick, Mary Ellen; O'Hara, Andrea J.; Le Gallo, Matthieu; Cruz, Pedro; Zhang, Suiyuan; Mullikin, James; Bell, Daphne W.; NISC Comparative Sequencing Progra] NHGRI, NIH, Bethesda, MD 20892 USA. [Merino, Maria J.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5312 DI 10.1158/1538-7445.AM2013-5312 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605334 ER PT J AU Ryan, BM Robles, AI McClary, AC Bowman, E Vahakangas, K Olivo-Marston, S Yang, P Jen, J Harris, CC AF Ryan, Brid M. Robles, Ana I. McClary, Andrew C. Bowman, Elise Vahakangas, Kirsi Olivo-Marston, Susan Yang, Ping Jen, Jin Harris, Curtis C. TI Interaction between DRD1 and childhood exposure to environmental tobacco smoke modulates lung cancer risk in smokers and never smokers. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Ryan, Brid M.; Robles, Ana I.; McClary, Andrew C.; Bowman, Elise; Vahakangas, Kirsi; Harris, Curtis C.] NCI, Bethesda, MD 20892 USA. [Olivo-Marston, Susan] Ohio State Univ, Columbus, OH 43210 USA. [Yang, Ping; Jen, Jin] Mayo Clin, Rochester, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4581 DI 10.1158/1538-7445.AM2013-4581 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600316 ER PT J AU Sakai, H Redon, CE Bonner, WM Appella, E Mazur, S AF Sakai, Hiroyasu Redon, Christophe E. Bonner, William M. Appella, Ettore Mazur, Sharlyn TI Wild-type p53-induced phosphatase 1 (Wip1) prevents cellular senescence at physiological oxygen levels by regulating DNA damage signaling during DNA replication SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Sakai, Hiroyasu; Redon, Christophe E.; Bonner, William M.; Appella, Ettore; Mazur, Sharlyn] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4041 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603273 ER PT J AU Sakai, T Kovalchuk, AL Morse, H AF Sakai, Tomomi Kovalchuk, Alexander L. Morse, Herbert TI BcI-xL and IL-6 act independently and synergistically to accelerate plasmacytopoiesis and plasma cell tumor formation in Balb/c mice SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Sakai, Tomomi; Kovalchuk, Alexander L.; Morse, Herbert] NIAID, NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3852 DI 10.1158/1538-7445.AM2013-3852 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603086 ER PT J AU Saloustros, E Salpea, P Gugglioti, L Tsang, K Horvath, A Nesterova, M Qi, CF Morse, HC Stratakis, CA AF Saloustros, Emmanouil Salpea, Paraskevi Gugglioti, Lina Tsang, Kittman Horvath, Anelia Nesterova, Maria Qi, Chen-Feng Morse, Herbert C., III Stratakis, Constantine A. TI Novel hematopoietic neoplasms in prkar2a-deficient mice SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Saloustros, Emmanouil; Salpea, Paraskevi; Gugglioti, Lina; Tsang, Kittman; Horvath, Anelia; Nesterova, Maria; Stratakis, Constantine A.] NICHD, Sect Endocrinol & Genet, NIH, Bethesda, MD USA. [Qi, Chen-Feng; Morse, Herbert C., III] NICHD, Immunogenet Lab, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3854 DI 10.1158/1538-7445.AM2013-3854 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603088 ER PT J AU Savage, SA Mirabelio, L Wang, ZM Gastier-Foster, J Gorlick, R Khanna, C Flanagan, AM Tirabosco, R Andrulis, IL Wunder, J Gokgoz, N Patino-Garcia, A Sierrasesumaga, L Lecanda, F Kurucu, N Ilhan, IE Sari, N Serra, M Hattinger, C Picci, P Spector, L Barkauskas, DA Marina, N de Toledo, SC Petrilli, S Amary, MF Halai, D Thomas, D Douglass, C Meltzer, P Jacobs, K Chung, CC Berndt, SI Purdue, MP Caporaso, NE Tucker, M Rothman, N Landi, MT Silverman, DT Kraft, P Hunter, DJ Malats, N Kogevinas, M Wacholder, S Troisi, R Helman, L Fraumeni, JF Yeager, M Hoover, R Chanock, SJ AF Savage, Sharon A. Mirabelio, Lisa Wang, Zhaoming Gastier-Foster, Julie Gorlick, Richard Khanna, Chand Flanagan, Adrienne M. Tirabosco, Roberto Andrulis, Irene L. Wunder, Jay Gokgoz, Nalan Patino-Garcia, Ana Sierrasesumaga, Luis Lecanda, Fernando Kurucu, Nilgun Ilhan, Inci Ergurhan Sari, Neriman Serra, Massimo Hattinger, Claudia Picci, Piero Spector, Logan Barkauskas, Donald A. Marina, Neyssa de Toledo, Siliva Caminada Petrilli, Sergio Amary, Maria Fernanda Halai, Dina Thomas, David Douglass, Chester Meltzer, Paul Jacobs, Kevin Chung, Charles C. Berndt, Sonja I. Purdue, Mark P. Caporaso, Neil E. Tucker, Margaret Rothman, Nathaniel Landi, Maria Teresa Silverman, Debra T. Kraft, Peter Hunter, David J. Malats, Nuria Kogevinas, Manolis Wacholder, Sholom Troisi, Rebecca Helman, Lee Fraumeni, Joseph F., Jr. Yeager, Meredith Hoover, Robert Chanock, Stephen J. TI Genome-wide association study identifies novel loci associated with osteosarcoma SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Savage, Sharon A.; Mirabelio, Lisa; Chung, Charles C.; Berndt, Sonja I.; Purdue, Mark P.; Caporaso, Neil E.; Tucker, Margaret; Rothman, Nathaniel; Landi, Maria Teresa; Silverman, Debra T.; Wacholder, Sholom; Troisi, Rebecca; Fraumeni, Joseph F., Jr.; Hoover, Robert; Chanock, Stephen J.] NCI, Rockville, MD USA. [Wang, Zhaoming; Jacobs, Kevin; Yeager, Meredith] NCI, SAIC Frederick Inc, Frederick, MD 21701 USA. [Gastier-Foster, Julie] Nationwide Childrens Hosp, Columbus, OH USA. [Gorlick, Richard] Albert Einstein Coll Med, Bronx, NY 10467 USA. [Khanna, Chand; Meltzer, Paul; Helman, Lee] NCI, CCR, Bethesda, MD 20892 USA. [Flanagan, Adrienne M.] UCL Canc Inst, Manchester, NH USA. [Flanagan, Adrienne M.; Tirabosco, Roberto; Amary, Maria Fernanda; Halai, Dina] Royal Natl Orthopaed Hosp NHS Trust, Manchester, Lancs, England. [Andrulis, Irene L.; Wunder, Jay; Gokgoz, Nalan] Univ Toronto, Toronto, ON, Canada. [Patino-Garcia, Ana; Sierrasesumaga, Luis; Lecanda, Fernando] Univ De Navarra, Pamplona, Spain. [Kurucu, Nilgun; Ilhan, Inci Ergurhan; Sari, Neriman] AY Ankara Oncol Training & Res Hosp, Ankara, Turkey. [Serra, Massimo; Hattinger, Claudia; Picci, Piero] Orthopaed Rizzoli Inst, Bologna, Italy. [Spector, Logan] Univ Minnesota, Minneapolis, MN USA. [Barkauskas, Donald A.] Univ So Calif, Keck Sch Med, Los Angeles, CA 90033 USA. [Marina, Neyssa] Stanford Univ, Palo Alto, CA 94304 USA. [Marina, Neyssa] Lucile Packard Childrens Hosp, Palo Alto, CA USA. [de Toledo, Siliva Caminada; Petrilli, Sergio] GRAACC UNIFESP, Sao Paulo, Brazil. [Thomas, David] Univ Melbourne, Melbourne, Vic, Australia. [Douglass, Chester; Kraft, Peter; Hunter, David J.] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. [Malats, Nuria] Ctr Nacl Invest Oncol, Madrid, Spain. [Kogevinas, Manolis] Ctr Res Environm Epidemiol, Barcelona, Spain. RI Tucker, Margaret/B-4297-2015; Patino-Garcia, Ana/I-4299-2012; Purdue, Mark/C-9228-2016; Savage, Sharon/B-9747-2015; Hattinger, Claudia/Q-1212-2016; Kogevinas, Manolis/C-3918-2017 OI Purdue, Mark/0000-0003-1177-3108; Savage, Sharon/0000-0001-6006-0740; Hattinger, Claudia/0000-0002-9316-5095; NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4593 DI 10.1158/1538-7445.AM2013-4593 PG 2 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604179 ER PT J AU Shim, E Liu, D Gibbons, A Poirier, MC Liu, YM AF Shim, Eunwoo Liu, Daniel Gibbons, Alexander Poirier, Miriam C. Liu, Yongmin TI Overexpression of PGC-1 alpha in rat cardiomyocytes protects against nucleoside reverse transcriptase inhibitor (NRTI)-induced mitochondrial toxicity. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Shim, Eunwoo; Liu, Daniel; Gibbons, Alexander; Poirier, Miriam C.; Liu, Yongmin] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4418 DI 10.1158/1538-7445.AM2013-4418 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604023 ER PT J AU Sholler, GL Bergendahl, G VanderWerff, A Ferguson, W Roberts, W Eslin, D Kraveka, J Kaplan, J Mitchell, D Parikh, N Neville, K Ashikaga, T Bond, J Hanna, G Merchant, M Huentelman, M Corneveaux, J Trent, J AF Sholler, Giselle L. Bergendahl, Genevieve VanderWerff, Alyssa Ferguson, William Roberts, William Eslin, Don Kraveka, Jacqueline Kaplan, Joel Mitchell, Deanna Parikh, Nehal Neville, Kathleen Ashikaga, Takamaru Bond, Jeffrey Hanna, Gina Merchant, Melinda Huentelman, Matt Corneveaux, Jason Trent, Jeffrey TI A feasibility trial using molecular-guided therapy for the treatment of patients with refractory or recurrent neuroblastoma. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Sholler, Giselle L.; Bergendahl, Genevieve; VanderWerff, Alyssa] Van Andel Res Inst, Grand Rapids, MI USA. [Ferguson, William] Cardinal Glennon Childrens Med Ctr, St Louis, MO USA. [Roberts, William] Rady Childrens Hosp, San Diego, CA USA. [Eslin, Don; Hanna, Gina] Arnold Palmer Hosp Children, Orlando, FL USA. [Kraveka, Jacqueline] Med Univ S Carolina, Charleston, SC 29425 USA. [Kaplan, Joel] Levine Childrens Hosp, Charlotte, NC USA. [Mitchell, Deanna] Helen DeVos Childrens Hosp, Grand Rapids, MI USA. [Parikh, Nehal] Connecticut Childrens Hosp, Hartford, CT USA. [Neville, Kathleen] Childrens Mercy Hosp & Clin, Kansas City, MO USA. [Ashikaga, Takamaru; Bond, Jeffrey] Univ Vermont, Burlington, VT USA. [Merchant, Melinda] NCI, Bethesda, MD 20892 USA. [Huentelman, Matt; Corneveaux, Jason; Trent, Jeffrey] Translat Genom Inst, Phoenix, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB160 DI 10.1158/1538-7445.AM2013-LB-160 PG 2 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601096 ER PT J AU Smith, M Kang, M Reynolds, P Gorlick, R Kolb, A Maris, J Keir, S Billups, C Kurmasheva, R Houghton, P AF Smith, Malcolm Kang, Min Reynolds, Patrick Gorlick, Richard Kolb, Anders Maris, John Keir, Stephen Billups, Catherine Kurmasheva, Raushan Houghton, Peter TI Pediatric Preclinical Testing Program (PPTP) stage I evaluation of cabozantinib SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Smith, Malcolm] NCI, Bethesda, MD 20892 USA. [Kang, Min; Reynolds, Patrick] Texas Tech Univ, Hlth Sci Ctr, Lubbock, TX 79430 USA. [Gorlick, Richard] Montefiore Med Ctr, Bronx, NY 10467 USA. [Kolb, Anders] Alfred I DuPont Hosp Children, Wilmington, DE USA. [Maris, John] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Keir, Stephen] Duke Univ, Med Ctr, Durham, NC USA. [Billups, Catherine] St Jude Childrens Res Hosp, Memphis, TN 38105 USA. [Kurmasheva, Raushan; Houghton, Peter] Nationwide Childrens Hosp Ctr Childhood Canc, Columbus, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB353 DI 10.1158/1538-7445.AM2013-LB-353 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601279 ER PT J AU Spector, LG Ritter, K Demerath, EW Sklar, C Ross, JA Krailo, M Nagarajan, R Malkin, D Bergemann, TL Savage, SA Johnson, W AF Spector, Logan G. Ritter, Kathryn Demerath, Ellen W. Sklar, Charles Ross, Julie A. Krailo, Mark Nagarajan, Rajaram Malkin, David Bergemann, Tracy L. Savage, Sharon A. Johnson, William TI Pediatric osteosarcoma patients are taller than average from birth to age twelve: a report from the Children's Oncology Group SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Spector, Logan G.; Ritter, Kathryn; Demerath, Ellen W.; Ross, Julie A.; Johnson, William] Univ Minnesota, Minneapolis, MN USA. [Sklar, Charles] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. [Krailo, Mark] Univ So Calif, Los Angeles, CA USA. [Nagarajan, Rajaram] Cincinnati Childrens Hosp, Cincinnati, OH USA. [Malkin, David] Hosp Sick Children, Toronto, ON M5G 1X8, Canada. [Bergemann, Tracy L.] Medtronic, Mounds View, MN USA. [Savage, Sharon A.] NCI, Bethesda, MD 20892 USA. RI Savage, Sharon/B-9747-2015 OI Savage, Sharon/0000-0001-6006-0740 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2532 DI 10.1158/1538-7445.AM2013-2532 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600171 ER PT J AU Srivastava, AK Jaganathan, S Stephen, LL Hollingshead, MG Scull, J Kinders, RJ Damour, E Donohue, J Layhee, A Mapes, J Esposito, D Tomaszewski, JE Parchment, RE Doroshow, JH AF Srivastava, Apurva K. Jaganathan, Soumya Stephen, Laurie L. Hollingshead, Melinda G. Scull, Jason Kinders, Robert J. Damour, Eric Donohue, Jennifer Layhee, Adam Mapes, James Esposito, Dominic Tomaszewski, Joseph E. Parchment, Ralph E. Doroshow, James H. TI Progress on development of multiplex panel of 15 biomarkers to support development of anticancer drugs targeting apoptosis. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Srivastava, Apurva K.; Jaganathan, Soumya; Hollingshead, Melinda G.; Kinders, Robert J.; Esposito, Dominic; Parchment, Ralph E.] Frederick Natl Lab Canc Res, Frederick, MD USA. [Stephen, Laurie L.; Scull, Jason; Damour, Eric; Donohue, Jennifer; Layhee, Adam] RBM Myriad, Lake Placid, NY USA. [Mapes, James] RBM Myriad, Austin, TX USA. [Tomaszewski, Joseph E.; Doroshow, James H.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3366 DI 10.1158/1538-7445.AM2013-3366 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602073 ER PT J AU Stein, WD Wilkerson, J Manasanch, E Zhuang, SH Bates, SE Fojo, T AF Stein, Wilfred D. Wilkerson, Julia Manasanch, Elisabet Zhuang, Sen H. Bates, Susan E. Fojo, Tito TI Estimating the fraction of a tumor that is killed by a drug. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Stein, Wilfred D.; Wilkerson, Julia; Bates, Susan E.; Fojo, Tito] Mol Oncol Branch, Bethesda, MD USA. [Manasanch, Elisabet] NCI, NIH, Bethesda, MD 20892 USA. [Zhuang, Sen H.] Johnson & Johnson, Clin Oncol Grp, Raritan, NJ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5152 DI 10.1158/1538-7445.AM2013-5152 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605248 ER PT J AU Su, WH Chang, KP Shugart, YY Chang, YS AF Su, Wen-Hui Chang, Kai-Ping Shugart, Yin Yao Chang, Yu-Sun TI Exploration of genome-wide two-locus SNP-SNP interactions relate to nasopharyngeal carcinoma susceptibility. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Su, Wen-Hui; Chang, Kai-Ping; Chang, Yu-Sun] Chang Gung Univ, Tao Yuan, Taiwan. [Shugart, Yin Yao] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4838 DI 10.1158/1538-7445.AM2013-4838 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600370 ER PT J AU Tarasova, N Chen, YH Dyba, M Tarasov, SG AF Tarasova, Nadya Chen, Yuhong Dyba, Marzena Tarasov, Sergey G. TI Fully synthetic self-assembling tumor-targeted virus-like nanoparticles SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Tarasova, Nadya; Chen, Yuhong; Dyba, Marzena; Tarasov, Sergey G.] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB-3 DI 10.1158/1538-7445.AM2013-LB-3 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601020 ER PT J AU Thomas, A Lee, JH Wang, YS Abdullaev, Z Saidkhodjaeva, L Pack, SD Giaccone, G AF Thomas, Anish Lee, Jih-Hsiang Wang, Yisong Abdullaev, Ziedulla Saidkhodjaeva, Lola Pack, Svetlana D. Giaccone, Giuseppe TI The role of fibroblast growth factor receptor 1 (FGFR1) in small cell lung cancer (SCLC). SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Thomas, Anish; Lee, Jih-Hsiang; Wang, Yisong; Abdullaev, Ziedulla; Saidkhodjaeva, Lola; Pack, Svetlana D.; Giaccone, Giuseppe] NCI, Bethesda, MD 20892 USA. RI Pack, Svetlana/C-2020-2014 NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5466 DI 10.1158/1538-7445.AM2013-5466 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605345 ER PT J AU Turbyville, T Brafman, A Chen, D Romanchuck, K Beutler, J Reilly, K Lockett, S AF Turbyville, Thomas Brafman, Alla Chen, De Romanchuck, Kathryn Beutler, John Reilly, Karlyne Lockett, Stephen TI Coordination of cell cycle repression and reduced cytoskeletal tension by the small molecule natural product, Schweinfurthin A. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Turbyville, Thomas; Brafman, Alla; Chen, De; Romanchuck, Kathryn; Lockett, Stephen] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Opt Microscopy & Image Anal Lab, Frederick, MD USA. [Beutler, John] NCI, Mol Targets Lab, Ctr Canc Res, Frederick, MD 21701 USA. [Reilly, Karlyne] NCI, Mouse Canc Genet Program, Ctr Canc Res, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3429 DI 10.1158/1538-7445.AM2013-3429 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602135 ER PT J AU Vander Broek, R Bian, YS Herzog, A Hall, B Coupar, J Chen, Z Kulkarni, AB Van Waes, C AF Vander Broek, Robert Bian, Yansong Herzog, Amanda Hall, Bradford Coupar, Jamie Chen, Zhong Kulkarni, Ashok B. Van Waes, Carter TI Preclinical investigation of antitumor effects of dual PI3K/mTOR inhibitor PF-04691502 in human xenograft and murine Pten/Tgfbr1 deficient head and neck cancer models. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Vander Broek, Robert; Bian, Yansong; Herzog, Amanda; Coupar, Jamie; Chen, Zhong; Van Waes, Carter] Natl Inst Deafness & Other Commun Disorders, NIH, Bethesda, MD USA. [Hall, Bradford; Kulkarni, Ashok B.] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB314 DI 10.1158/1538-7445.AM2013-LB-314 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601240 ER PT J AU Villalona-Calero, MA Duan, WR Zhao, WQ Shilo, K Ji, JP Thurmond, J Norris, A Rose, J Layman, R Marshall, J Bekaii-Saab, T Chen, A AF Villalona-Calero, Miguel A. Duan, Wenrui Zhao, Weiqiang Shilo, Konstantin Ji, Jiuping Thurmond, Jennifer Norris, Adam Rose, Jeffrey Layman, Rachel Marshall, John Bekaii-Saab, Tanios Chen, Alice TI Phase I trial of veliparib or mitomycin (M MC) plus veliparib in patients with sporadic solid tumors screened for somatic deficiency in the Fanconi Anemia (FA) pathway. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Villalona-Calero, Miguel A.; Duan, Wenrui; Zhao, Weiqiang; Shilo, Konstantin; Thurmond, Jennifer; Norris, Adam; Rose, Jeffrey; Layman, Rachel; Bekaii-Saab, Tanios] Ohio State Univ, Med Ctr, Columbus, OH 43210 USA. [Ji, Jiuping; Chen, Alice] NCI, Bethesda, MD 20892 USA. [Marshall, John] Georgetown Univ, Washington, DC USA. RI Bekaii-Saab, Tanios/E-2733-2011; Layman, Rachel/E-3475-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB139 DI 10.1158/1538-7445.AM2013-LB-139 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601079 ER PT J AU Walden, CA Reid, JM McGovern, RM Covey, JM Ames, MM AF Walden, Chad A. Reid, Joel M. McGovern, Renee M. Covey, Joseh M. Ames, Matthew M. TI LC/MS/MS assay and mouse pharmacokinetics of the phenylurea thiocarbamate NSC 161128 SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Walden, Chad A.; Reid, Joel M.; McGovern, Renee M.; Ames, Matthew M.] Mayo Clin, Rochester, MN USA. [Covey, Joseh M.] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4557 DI 10.1158/1538-7445.AM2013-4557 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604161 ER PT J AU Wang, TW Lan, Q Krystyna, B Rothman, N Florido, R Hu, W Steiling, K Liu, G Xiao, J Alekseyev, Y Xu, J Wei, FS Hosgood, HD Reiss, B Downward, G Lenburg, M Vermeulen, R Spira, A AF Wang, Teresa W. Lan, Qing Krystyna, Bozena Rothman, Nathaniel Florido, Roberta Hu, Wei Steiling, Katrina Liu, Gang Xiao, Ji Alekseyev, Yuriy Xu, Jun Wei, Fusheng Hosgood, H. Dean Reiss, Boris Downward, George Lenburg, Marc Vermeulen, Roel Spira, Avrum TI Transcriptomic changes in the oral mucosal epithelium reflect the physiologic response to indoor burning of solid fuels SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wang, Teresa W.; Krystyna, Bozena; Florido, Roberta; Steiling, Katrina; Liu, Gang; Xiao, Ji; Alekseyev, Yuriy; Lenburg, Marc; Spira, Avrum] Boston Univ, Sch Med, Boston, MA 02118 USA. [Lan, Qing; Rothman, Nathaniel; Hu, Wei] NCI, Bethesda, MD 20892 USA. [Xu, Jun] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China. [Wei, Fusheng] China Natl Environm Monitoring Ctr, Beijing, Peoples R China. [Hosgood, H. Dean] Albert Einstein Coll Med, Bronx, NY 10467 USA. [Reiss, Boris] Univ Washington, Seattle, WA 98195 USA. [Downward, George; Vermeulen, Roel] Univ Utrecht, Utrecht, Netherlands. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 112 DI 10.1158/1538-7445.AM2013-112 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600029 ER PT J AU Wang, XT Pittman, GS Su, D Adamski, KN Campbell, MR Joubert, BR Huang, ZQY Hoyo, C Murphy, SK London, SA Bell, DA AF Wang, Xuting Pittman, Gary S. Su, Dan Adamski, Kelly N. Campbell, Michelle R. Joubert, Bonnie R. Huang, Zhiqing Y. Hoyo, Cathrine Murphy, Susan K. London, Stephanie A. Bell, Douglas A. TI Dose-dependent alteration of CpG methylation in AHRR and GFI1 in mononuclear cell DNA of smokers. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wang, Xuting; Pittman, Gary S.; Su, Dan; Adamski, Kelly N.; Campbell, Michelle R.; Joubert, Bonnie R.; London, Stephanie A.; Bell, Douglas A.] NIEHS, NIH, Res Triangle Pk, NC 27709 USA. [Huang, Zhiqing Y.; Hoyo, Cathrine; Murphy, Susan K.] Duke Univ, Durham, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3647 DI 10.1158/1538-7445.AM2013-3647 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600267 ER PT J AU Wang, Y Cheryan, VT Jamal, S Chen, D Yang, HJ Polin, LA Pass, HI Dou, QP Sharma, S Rishi, AK Wali, A AF Wang, Ying Cheryan, Vino T. Jamal, Shazia Chen, Di Yang, Huanjie Polin, Lisa A. Pass, Harvey I. Dou, Q. Ping Sharma, Sunita Rishi, Arun K. Wali, Anil TI Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wang, Ying; Cheryan, Vino T.; Jamal, Shazia; Chen, Di; Polin, Lisa A.; Dou, Q. Ping] Wayne State Univ, Dept Oncol, Karmanos Canc Inst, Detroit, MI USA. [Yang, Huanjie] Harbin Inst Technol, Dept Life Sci & Engn, Harbin 150006, Peoples R China. [Pass, Harvey I.] NYU, Ctr Canc, Div Cardiothorac Surg, New York, NY USA. [Sharma, Sunita] Wayne State Univ, Dept Surg, John D Dingell VA Med Ctr, Detroit, MI USA. [Rishi, Arun K.] Wayne State Univ, Dept Oncol, John D Dingell VA Med Ctr, Detroit, MI USA. [Wali, Anil] Natl Canc Inst, Natl Inst Hlth, Ctr Reduce Canc Hlth Dispar, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5564 DI 10.1158/1538-7445.AM2013-5564 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220606043 ER PT J AU Wang, ZY Martin, D Patel, V Molinolo, AA Chen, QM Gutkind, JS AF Wang, Zhiyong Martin, Daniel Patel, Vyomesh Molinolo, Alfredo Alberto Chen, Qianming Gutkind, J. Silvio TI mTOR inhibition can overcome cetuximab resistance in head and neck cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wang, Zhiyong; Martin, Daniel; Patel, Vyomesh; Molinolo, Alfredo Alberto; Gutkind, J. Silvio] NIDCR, Oral & Pnaryngeal Canc Branch, NIH, Bethesda, MD USA. [Chen, Qianming] Sichuan Univ, State Key Lab Oral Dis, West China Hosp Stomatol, Chengdu 610064, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB224 DI 10.1158/1538-7445.AM2013-LB-224 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601154 ER PT J AU Weinstein, SJ Purdue, MP Smith-Warner, SA Mondul, AM Black, A Horst, RL Ziegler, RG Albanes, D AF Weinstein, Stephanie J. Purdue, Mark P. Smith-Warner, Stephanie A. Mondul, Alison M. Black, Amanda Horst, Ronald L. Ziegler, Regina G. Albanes, Demetrius TI Serum vitamin D, vitamin D binding protein, and incident colorectal cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO) cohort SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Weinstein, Stephanie J.; Purdue, Mark P.; Mondul, Alison M.; Black, Amanda; Ziegler, Regina G.; Albanes, Demetrius] Natl Canc Inst, Bethesda, MD USA. [Smith-Warner, Stephanie A.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Bethesda, MD USA. [Horst, Ronald L.] Heartland Assays Inc, Ames, IA USA. RI Albanes, Demetrius/B-9749-2015; Purdue, Mark/C-9228-2016 OI Purdue, Mark/0000-0003-1177-3108 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4824 DI 10.1158/1538-7445.AM2013-4824 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600357 ER PT J AU Wentzensen, NA Bakkum, J Killian, K Shridhar, V Adams, L Guido, R D'Ambrosio, L Luhn, P Sampson, J Yang, H Brinton, L Lissowska, J Podratz, K Sherman, M AF Wentzensen, Nicolas A. Bakkum, Jamie Killian, Keith Shridhar, Viji Adams, Lisa Guido, Richard D'Ambrosio, Lori Luhn, Patricia Sampson, Joshua Yang, Hannah Brinton, Louise Lissowska, Jolanta Podratz, Karl Sherman, Mark TI Discovery and validation of methylation markers for early detection of endometrial cancer. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wentzensen, Nicolas A.; Luhn, Patricia; Sampson, Joshua; Yang, Hannah; Brinton, Louise; Sherman, Mark] NCI DCEG, Bethesda, MD USA. [Bakkum, Jamie; Shridhar, Viji; Podratz, Karl] Mayo Clin, Rochester, MN USA. [Killian, Keith; Adams, Lisa] NCI CCR, Bethesda, MD USA. [Guido, Richard; D'Ambrosio, Lori] UPMC Syst, Magee Womens Hosp, Pittsburgh, PA USA. [Lissowska, Jolanta] Sklodowska Curie Mem Canc Ctr, Warsaw, Poland. [Lissowska, Jolanta] Inst Oncol, Warsaw, Poland. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3477 DI 10.1158/1538-7445.AM2013-3477 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602182 ER PT J AU Wiestner, A Herman, S Mustafa, R Valdez, J Jones, J Saba, N Lipsky, A Arthur, DC Marti, G Thomas, F Maric, I Pittaluga, S Tian, X Soto, S Aue, G Farooqui, MZ AF Wiestner, Adrian Herman, Sarah Mustafa, Rashida Valdez, Janet Jones, Jade Saba, Nakhle Lipsky, Andrew Arthur, Diane C. Marti, Gerald Thomas, Francine Maric, Irina Pittaluga, Stefania Tian, Xin Soto, Susan Aue, Georg Farooqui, Mohammed Z. TI Potent single agent activity of Ibrutinib (PCI-32765) in patients with chronic lymphocytic leukemia (CLL): clinical and translational results from an ongoing phase II study. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wiestner, Adrian; Herman, Sarah; Mustafa, Rashida; Valdez, Janet; Jones, Jade; Saba, Nakhle; Lipsky, Andrew; Marti, Gerald; Soto, Susan; Aue, Georg; Farooqui, Mohammed Z.] NHLBI, NIH, Bethesda, MD 20892 USA. [Arthur, Diane C.] NCI, NIH, Pathol Lab, CCR, Bethesda, MD 20892 USA. [Thomas, Francine] NIH, RIS, CRC, Bethesda, MD 20892 USA. [Maric, Irina] NIH, DLM, CCR, Bethesda, MD 20892 USA. [Pittaluga, Stefania] NCI, NIH, Pathol Lab, Bethesda, MD 20892 USA. [Tian, Xin] NHLBI, NIH, Off Biostat Res, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB141 DI 10.1158/1538-7445.AM2013-LB-141 PG 2 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601081 ER PT J AU Woditschka, S Palmieri, D Duchnowska, R Jassem, J Badve, S Sledge, GW Steeg, PS AF Woditschka, Stephan Palmieri, Diane Duchnowska, Renata Jassem, Jacek Badve, Sunil Sledge, George W. Steeg, Patricia S. TI Overexpression of RAD51 promotes brain metastases from breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Woditschka, Stephan; Palmieri, Diane; Steeg, Patricia S.] NCI, Bethesda, MD 20892 USA. [Duchnowska, Renata] Mil Inst Med, Warsaw, Poland. [Jassem, Jacek] Med Univ Gdansk, Gdansk, Poland. [Badve, Sunil; Sledge, George W.] Indiana Univ Sch Med, Indianapolis, IN 46202 USA. RI Palmieri, Diane/B-4258-2015 NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4589 DI 10.1158/1538-7445.AM2013-4589 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604175 ER PT J AU Woloszynska-Read, A Arab, L Adams, J Bensen, JT Fontham, ETH Mohler, JL Su, J Tabung, F Zhang, HM Trump, DL Johnson, CS Steck, SE AF Woloszynska-Read, Anna Arab, Lenore Adams, John Bensen, Jeannette T. Fontham, Elizabeth T. H. Mohler, James L. Su, Joseph Tabung, Fred Zhang, Hongmei Trump, Donald L. Johnson, Candace S. Steck, Susan E. TI Plasma 25-hydroxyvitamin D levels are associated with aggressive prostate cancer among African Americans in the North Carolina-Louisiana Prostate Cancer Project (PCaP) SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Woloszynska-Read, Anna; Mohler, James L.; Trump, Donald L.; Johnson, Candace S.] Roswell Pk Canc Inst, Buffalo, NY 14263 USA. [Arab, Lenore; Adams, John] Univ Calif Los Angeles, Los Angeles, CA USA. [Bensen, Jeannette T.] Univ N Carolina, Chapel Hill, NC USA. [Fontham, Elizabeth T. H.] Louisiana State Univ, New Orleans, LA USA. [Su, Joseph] NCI, Bethesda, MD 20892 USA. [Tabung, Fred; Zhang, Hongmei; Steck, Susan E.] Univ S Carolina, Columbia, SC 29208 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB-12 DI 10.1158/1538-7445.AM2013-LB-12 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601027 ER PT J AU Wong, J Harris, J Rodriguez-Galindo, C Johnson, K AF Wong, Jeannette Harris, Jenine Rodriguez-Galindo, Carlos Johnson, Kimberly TI Incidence trends of childhood and adolescent melanoma in the United States from 1973-2009 SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wong, Jeannette] NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, Rockville, MD USA. [Harris, Jenine] Washington Univ, St Louis, MO USA. [Rodriguez-Galindo, Carlos] Harvard Univ, Dana Farber Canc Inst, Cambridge, MA 02138 USA. [Johnson, Kimberly] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2530 DI 10.1158/1538-7445.AM2013-2530 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600169 ER PT J AU Wood, LV Dahut, WL Fojo, A Gulley, JL Madan, R Arlen, P Parnes, HL Roberson, BD Steinberg, SM Terabe, M Wilkerson, J Pastan, I Berzofsky, JA AF Wood, Lauren V. Dahut, William L. Fojo, Antonio Gulley, James L. Madan, Ravi Arlen, Philip Parnes, Howard L. Roberson, Brenda D. Steinberg, Seth M. Terabe, Masaki Wilkerson, Julia Pastan, Ira Berzofsky, Jay A. TI Autologous TARP peptide vaccination is associated with slowing in PSA velocity and a decrease in tumor growth rate in patients with Stage DO prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wood, Lauren V.; Dahut, William L.; Fojo, Antonio; Gulley, James L.; Madan, Ravi; Arlen, Philip; Parnes, Howard L.; Roberson, Brenda D.; Steinberg, Seth M.; Terabe, Masaki; Wilkerson, Julia; Pastan, Ira; Berzofsky, Jay A.] NCI, Bethesda, MD 20892 USA. RI Gulley, James/K-4139-2016 OI Gulley, James/0000-0002-6569-2912 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4571 DI 10.1158/1538-7445.AM2013-4571 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600306 ER PT J AU Wu, CP Sim, HM Huang, YH Liu, YC Hsiao, SH Cheng, HW Li, YQ Ambudkar, S Hsu, SC AF Wu, Chung-Pu Sim, Hong-May Huang, Yang-Hui Liu, Yen-Chen Hsiao, Sung-Han Cheng, Hsing-Wen Li, Yan-Qing Ambudkar, Suresh Hsu, Sheng-Chieh TI Overexpression of ATP-binding cassette transporter ABCG2 as a potential mechanism of acquired resistance to vemurafenib in BRAF(V600E) mutant cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Wu, Chung-Pu; Huang, Yang-Hui; Liu, Yen-Chen; Hsiao, Sung-Han; Cheng, Hsing-Wen; Li, Yan-Qing; Hsu, Sheng-Chieh] Chang Gung Univ, Tao Yuan, Taiwan. [Sim, Hong-May; Ambudkar, Suresh] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3398 DI 10.1158/1538-7445.AM2013-3398 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220602104 ER PT J AU Yang, HP Murphy, K George, N Garcia-Closas, M Lissowska, J Brinton, LA Wentzensen, N AF Yang, Hannah P. Murphy, Kelsey George, Neena Garcia-Closas, Montserrat Lissowska, Jolanta Brinton, Louise A. Wentzensen, Nicolas TI Lifetime ovulatory cycles and risk of ovarian and endometrial cancers SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Hannah P.; Murphy, Kelsey; Brinton, Louise A.; Wentzensen, Nicolas] NCI, Rockville, MD USA. [George, Neena] Drexel Univ, Philadelphia, PA 19104 USA. [Garcia-Closas, Montserrat] Inst Canc Res, London, England. [Lissowska, Jolanta] M Sklodowska Curie Canc Ctr, Krakow, Poland. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 154 DI 10.1158/1538-7445.AM2013-154 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600070 ER PT J AU Yang, HP Sherman, ME Schneider, SS Chisholm, CM Browne, EP Mahmood, S Lenington, S Anderton, DL Arcaro, KA AF Yang, Hannah P. Sherman, Mark E. Schneider, Sallie Smith Chisholm, Christina M. Browne, Eva P. Mahmood, Sidra Lenington, Sarah Anderton, Douglas L. Arcaro, Kathleen A. TI Association of TGF-beta 2 levels in breast milk with breast biopsy diagnosis. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Hannah P.; Sherman, Mark E.] NCI, Rockville, MD USA. [Schneider, Sallie Smith] Pioneer Valley Life Sci Inst, Springfield, MA USA. [Chisholm, Christina M.; Browne, Eva P.; Lenington, Sarah; Anderton, Douglas L.; Arcaro, Kathleen A.] Univ Massachusetts, Amherst, MA 01003 USA. [Mahmood, Sidra] Mt Holyoke Coll, S Hadley, MA 01075 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 117 DI 10.1158/1538-7445.AM2013-117 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600034 ER PT J AU Yang, H Volfovsky, N Rattray, A Chen, XF Tanaka, H Jeffrey, S AF Yang, Hui Volfovsky, Natalia Rattray, Alison Chen, Xiongfong Tanaka, Hisashi Jeffrey, Strathern TI Identification of DNA palindromes in the MCF-7 breast cancer cell line. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Hui; Rattray, Alison] Frederick Natl Lab Canc Res, Gene Regulat & Chromosome Biol Lab, Frederick, MD USA. [Volfovsky, Natalia; Chen, Xiongfong] SAIC Frederick Inc, Frederick Natl Lab Canc Res, ABCC ISP, Frederick, MD USA. [Tanaka, Hisashi] Cleveland Clin, Lerner Res Inst, Dept Mol Genet, Cleveland, MD USA. [Jeffrey, Strathern] Frederick Natl Lab, Gene Regulat & Chromosome Biol Lab, Frederick Natl Lab Canc Res, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB233 DI 10.1158/1538-7445.AM2013-LB-233 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601162 ER PT J AU Yang, XH Burke, L Jacobs, K Cullen, M Boland, J Burdett, L Malasky, M Rotunno, M Yeager, M Chanock, S Tucker, M Goldstein, A AF Yang, Xiaohong (Rose) Burke, Laura Jacobs, Kevin Cullen, Michael Boland, Joseph Burdett, Laurie Malasky, Michael Rotunno, Melissa Yeager, Meredith Chanock, Stephen Tucker, Margaret Goldstein, Alisa TI Characterization of rare germline variants in somatically mutated melanoma genes in melanoma-prone families. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Xiaohong (Rose); Burke, Laura; Jacobs, Kevin; Cullen, Michael; Boland, Joseph; Burdett, Laurie; Malasky, Michael; Rotunno, Melissa; Yeager, Meredith; Chanock, Stephen; Tucker, Margaret; Goldstein, Alisa] NCI, NIH, DHHS, Bethesda, MD 20892 USA. RI Tucker, Margaret/B-4297-2015 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 2553 DI 10.1158/1538-7445.AM2013-2553 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220600191 ER PT J AU Yang, X Xiao, Z Wang, JH Lu, Y Xu, Y Huang, X Mizokami, A Keller, ET Zhang, J AF Yang, Xin Xiao, Zhen Wang, Jianhua Lu, Yi Xu, Yang Huang, Xin Mizokami, Atsushi Keller, Evan T. Zhang, Jian TI IKK-Epsilon, a novel factor identified by proteomics, contributes to prostate cancer cell-induced osteoclast activity in vitro. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Xin; Lu, Yi; Huang, Xin; Zhang, Jian] Guangxi Med Univ, Minist Educ, Key Lab Ageing & Ageing Related Dis, Ctr Translat Med, Nanning, Peoples R China. [Xiao, Zhen] Frederick Natl Lab Canc Res, Frederick, MD USA. [Wang, Jianhua] Shanghai Jiao Tong Univ, Sch Med, Shanghai 200030, Peoples R China. [Xu, Yang] SELDI Biotechnol Ltd, Huzhou, Peoples R China. [Mizokami, Atsushi] Kanazawa Univ, Grad Sch Med Sci, Kanazawa, Ishikawa, Japan. [Keller, Evan T.] Univ Michigan, Ann Arbor, MI 48109 USA. RI Keller, Evan/M-1446-2016 OI Keller, Evan/0000-0002-7592-7535 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3945 DI 10.1158/1538-7445.AM2013-3945 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603179 ER PT J AU Yang, YA Weng, J Welsh, M Guan, N Webster, J Flanders, K Lonning, SM McPherson, J Wakefield, LM AF Yang, Yu-an Weng, Jia Welsh, Michael Guan, Nancy Webster, Josh Flanders, Kathy Lonning, Scott M. McPherson, John Wakefield, Lalage M. TI Heterogeneity of response to anti-TGF-beta antibody therapy in preclinical models SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yang, Yu-an; Weng, Jia; Welsh, Michael; Guan, Nancy; Webster, Josh; Flanders, Kathy; Wakefield, Lalage M.] NCI, Bethesda, MD 20892 USA. [Lonning, Scott M.; McPherson, John] Genzyme Corp, Framingham, MA 01701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 5484 DI 10.1158/1538-7445.AM2013-5484 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220605363 ER PT J AU Yu, YL Lu, A Dai, M Merlino, G AF Yu, Yanlin Lu, Andrew Dai, Meng Merlino, Glenn TI Identification of PHLPP1 as a novel metastasis suppressor in melanoma SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yu, Yanlin; Lu, Andrew; Dai, Meng; Merlino, Glenn] NCI CCR, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3868 DI 10.1158/1538-7445.AM2013-3868 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603102 ER PT J AU Yu, YK Kang, ZG Helman, L Meltzer, P Cao, L AF Yu, Yunkai Kang, Zhigang Helman, Lee Meltzer, Paul Cao, Liang TI Resistance to insulin-like growth factor 1 receptor antibody associated with downregulated IGFBP2. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yu, Yunkai; Kang, Zhigang; Helman, Lee; Meltzer, Paul; Cao, Liang] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 4449 DI 10.1158/1538-7445.AM2013-4449 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220604054 ER PT J AU Yuan, TL Lee, CS Fellmann, C Ritchie, C Lee, C Merrifield, C Schluep, T Lowe, SW Luo, J McCormick, F AF Yuan, Tina L. Lee, Chih-Shia Fellmann, Christof Ritchie, Cayde Lee, Changwoo Merrifield, Colin Schluep, Thomas Lowe, Scott W. Luo, Ji McCormick, Frank TI Nanoparticle-based RNAi therapy for the delivery of personalized siRNA payloads to KRAS-driven tumors. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Yuan, Tina L.; Ritchie, Cayde; McCormick, Frank] UCSF, San Francisco, CA USA. [Lee, Chih-Shia; Lee, Changwoo] NCI, Bethesda, MD 20892 USA. [Fellmann, Christof] Mirimus Inc, Cold Spring Harbor, NY USA. [Merrifield, Colin] Bioscale Inc, Lexington, MA USA. [Schluep, Thomas] Calando Pharmaceut, Pasadena, CA USA. [Lowe, Scott W.] Mem Sloan Kettering, New York, NY USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB94 DI 10.1158/1538-7445.AM2013-LB-94 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601340 ER PT J AU Zhang, PY Ungewitter, E Appella, E Scrable, H AF Zhang, Piyan Ungewitter, Erica Appella, Ettore Scrable, Heidi TI Tumor suppressor p53 is involved in a CHAMP-dependent spindle assembly checkpoint pathway. SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Zhang, Piyan; Scrable, Heidi] Mayo Clin, Rochester, MN USA. [Ungewitter, Erica] NIEHS, Res Triangle Pk, NC 27709 USA. [Appella, Ettore] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA LB121 DI 10.1158/1538-7445.AM2013-LB-121 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220601063 ER PT J AU Zhao, L Hyde, RK Alemu, L Liu, PP AF Zhao, Ling Hyde, R. Katherine Alemu, Lemiem Liu, P. Paul TI The interaction of RUNX1 with CBF beta-SMMHC during leukemogenesis SO CANCER RESEARCH LA English DT Meeting Abstract CT 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR) CY APR 06-10, 2013 CL Washington, DC SP Amer Assoc Canc Res C1 [Zhao, Ling; Hyde, R. Katherine; Alemu, Lemiem; Liu, P. Paul] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 SU 1 MA 3850 DI 10.1158/1538-7445.AM2013-3850 PG 1 WC Oncology SC Oncology GA AA6PP UT WOS:000331220603084 ER PT J AU Li, H Edin, ML Bradbury, JA Graves, JP DeGraff, LM Gruzdev, A Cheng, J Dackor, RT Wang, PM Bortner, CD Garantziotis, S Jetten, AM Zeldin, DC AF Li, Hong Edin, Matthew L. Bradbury, J. Alyce Graves, Joan P. DeGraff, Laura M. Gruzdev, Artiom Cheng, Jennifer Dackor, Ryan T. Wang, Ping Ming Bortner, Carl D. Garantziotis, Stavros Jetten, Anton M. Zeldin, Darryl C. TI Cyclooxygenase-2 Inhibits T Helper Cell Type 9 Differentiation during Allergic Lung Inflammation via Down-regulation of IL-17RB SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article DE T helper cell type 9 cells; cyclooxygenase 2; asthma; prostaglandins; IL-17RB ID BLOOD MONONUCLEAR-CELLS; PROSTAGLANDIN-D-2 RECEPTORS; IL-9 PRODUCTION; TGF-BETA; ASTHMA; EXPRESSION; PATHOGENESIS; INTERLEUKIN-9; CYTOKINES; RHINITIS AB Rationale: Helper CD4(+) T cell subsets, including IL-9-and IL-10-producing T helper cell type 9 (Th9) cells, exist under certain inflammatory conditions. Cyclooxygenase (COX)-1 and COX-2 play important roles in allergic lung inflammation and asthma. It is unknown whether COX-derived eicosanoids regulate Th9 cells during allergic lung inflammation. Objectives: To determine the role of COX metabolites in regulating Th9 cell differentiation and function during allergic lung inflammation. Methods: COX-1(-/-), COX-2(-/-), and wild-type (WT) mice were studied in an in vivo model of ovalbumin-induced allergic inflammation and an in vitro model of Th9 differentiation using flow cytometry, cytokine assays, confocal microscopy, real-time PCR, and immunoblotting. In addition, the role of specific eicosanoids and their receptors was examined using synthetic prostaglandins (PGs), selective inhibitors, and siRNA knockdown. Measurements and Main Results: Experimental endpoints were not different between COX-1(-/-) and WT mice; however, the percentage of IL-9(+) CD4(+) T cells was increased in lung, bronchoalveolar layage fluid, lymph nodes, and blood of allergic COX-2(-/-) mice relative to WT. Bronchoalyeolar lavage fluid IL-9 and IL-10, serum IL-9, and lung IL-17RB levels were significantly increased in allergic COX-2(-/-) mice or in WT mice treated with COX-2 inhibitors. IL-9, IL-10, and IL-17RB expression in vivo was inhibited by PGD(2) and PGE(2), which also reduced Th9 cell differentiation of murine and human naive CD4(+) T cells in vitro. Inhibition of protein kinase A significantly increased Th9 cell differentiation of naive CD4(+) T cells isolated from WT mice in vitro. Conclusions: COX-2-derived PGD(2) and PGE(2) regulate Th9 cell differentiation by suppressing IL-17RB expression via a protein kinase A-dependent mechanism. C1 [Li, Hong; Edin, Matthew L.; Bradbury, J. Alyce; Graves, Joan P.; DeGraff, Laura M.; Gruzdev, Artiom; Cheng, Jennifer; Dackor, Ryan T.; Garantziotis, Stavros; Jetten, Anton M.; Zeldin, Darryl C.] NIEHS, Lab Resp Biol, Div Intramural Res, NIH, Res Triangle Pk, NC 27709 USA. [Bortner, Carl D.] NIEHS, Lab Signal Transduct, Div Intramural Res, NIH, Res Triangle Pk, NC 27709 USA. [Wang, Ping Ming; Bortner, Carl D.] NICHHD, Unit Lung Injury & Repair, Div Intramural Res, NIH, Bethesda, MD 20892 USA. RP Zeldin, DC (reprint author), NIEHS, Div Intramural Res, NIH, 111 TW Alexander Dr,Bldg 101,Room A214, Res Triangle Pk, NC 27709 USA. EM zeldin@niehs.nih.gov RI Garantziotis, Stavros/A-6903-2009; OI Garantziotis, Stavros/0000-0003-4007-375X; Jetten, Anton/0000-0003-0954-4445; Edin, Matthew/0000-0002-7042-500X FU National Institutes of Health/National Institute of Environmental Health Sciences, Division of Intramural Research [Z01 ES025043, Z01 ES050167] FX Supported by National Institutes of Health/National Institute of Environmental Health Sciences, Division of Intramural Research grants Z01 ES025043 and Z01 ES050167. NR 50 TC 18 Z9 20 U1 1 U2 12 PU AMER THORACIC SOC PI NEW YORK PA 61 BROADWAY, FL 4, NEW YORK, NY 10006 USA SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR 15 PY 2013 VL 187 IS 8 BP 812 EP 822 DI 10.1164/rccm.201211-2073OC PG 11 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA 133MA UT WOS:000318141300010 PM 23449692 ER PT J AU Mehari, A Alam, S Tian, X Cuttica, MJ Barnett, CF Miles, G Xu, DH Seamon, C Adams-Graves, P Castro, OL Minniti, CP Sachdev, V Taylor, JG Kato, GJ Machado, RF AF Mehari, Alem Alam, Shoaib Tian, Xin Cuttica, Michael J. Barnett, Christopher F. Miles, George Xu, Dihua Seamon, Catherine Adams-Graves, Patricia Castro, Oswaldo L. Minniti, Caterina P. Sachdev, Vandana Taylor, James G. Kato, Gregory J. Machado, Roberto F. TI Hemodynamic Predictors of Mortality in Adults with Sickle Cell Disease SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article DE sickle cell; pulmonary hypertension; mortality; autopsy ID PULMONARY ARTERIAL-HYPERTENSION; LUNG-DISEASE; PORTOPULMONARY HYPERTENSION; CLINICAL CLASSIFICATION; VASOOCCLUSIVE PAIN; RISK-FACTOR; SURVIVAL; DEATH; REGISTRY; TRANSPLANTATION AB Background: Pulmonary hypertension (PH) in adults with sickle cell disease (SCD) is associated with early mortality, but no prior studies have evaluated quantitative relationships of mortality to physiological measures of pre- and postcapillary PH. Objectives: To identify risk factors associated with mortality and to estimate the expected survival in a cohort of patients with SCD with PH documented by right heart catheterization. Methods: Nine-year follow-up data (median, 4.7 yr) from the National Institutes of Health SCD PH screening study are reported. A total of 529 adults with SCD were screened by echocardiography between 2001 and 2010 with no exclusion criteria. Hemodynamic data were collected from 84 patients. PH was defined as mean pulmonary artery pressure (PAP) >= 25 mm Hg. Survival rates were estimated by the Kaplan-Meier method, and mortality risk factors were analyzed by the Cox proportional hazards regression. Measurements and Main Results: Specific hemodynamic variables were independently related to mortality: mean PAP (hazard ratio [HR], 1.61; 95% confidence interval [CI], 1.05-2.45 per 10 mm Hg increase; P = 0.027), diastolic PAP (HR, 1.83; 95% CI, 1.09-3.08 per 10 mm Hg increase; P = 0.022), diastolic PAP pulmonary capillary wedge pressure (HR, 2.19; 95% CI, 1.23-3.89 per 10 mm Hg increase; P = 0.008), transpulmonary gradient (HR, 1.78; 95% CI, 1.14-2.79 per 10 mm Hg increase; P = 0.011), and pulmonary vascular resistance (HR, 1.44; 95%. CI, 1.09-1.89 per Wood unit increase; P = 0.009) as risk factors for mortality. Conclusions: Mortality in adults with SCD and PH is proportional to the physiological severity of precapillary PH, demonstrating its prognostic and clinical relevance despite anemia-induced high cardiac output and less severely elevated pulmonary vascular resistance. C1 [Mehari, Alem; Alam, Shoaib; Castro, Oswaldo L.; Sachdev, Vandana] NHLBI, Cardiovasc & Pulm Branch, Bethesda, MD 20892 USA. [Mehari, Alem; Castro, Oswaldo L.] Howard Univ, Coll Med, Washington, DC USA. [Tian, Xin; Xu, Dihua] NHLBI, Off Biostat Res, Bethesda, MD 20892 USA. [Cuttica, Michael J.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. [Barnett, Christopher F.] Univ Calif San Francisco, Div Cardiol, San Francisco, CA USA. [Miles, George] NCI, Pathol Lab, Bethesda, MD 20892 USA. [Seamon, Catherine; Minniti, Caterina P.; Taylor, James G.; Kato, Gregory J.] NHLBI, Hematol Branch, Bethesda, MD 20892 USA. [Adams-Graves, Patricia] Univ Tennessee, Ctr Hlth Sci, Memphis, TN 38163 USA. [Machado, Roberto F.] Univ Illinois, Sect Pulm Crit Care Med Sleep & Allergy, Chicago, IL USA. RP Kato, GJ (reprint author), NHLBI, Hematol Branch, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM gkato@mail.nih.gov RI Kato, Gregory/I-7615-2014; OI Kato, Gregory/0000-0003-4465-3217; Taylor, James/0000-0002-4421-1809 FU Division of Intramural Research of the National Heart, Blood and Lung Institute of the National Institutes of Health [1ZIAHL006011, 1ZIAHL006015, 1ZIAH L006012]; National Institutes of Health [K23HL098454] FX This research was supported by the Division of Intramural Research of the National Heart, Blood and Lung Institute of the National Institutes of Health (grants 1ZIAHL006011, 1ZIAHL006015, and 1ZIAH L006012) and by National Institutes of Health grant K23HL098454 (R.F.M.). NR 52 TC 30 Z9 31 U1 0 U2 3 PU AMER THORACIC SOC PI NEW YORK PA 25 BROADWAY, 18 FL, NEW YORK, NY 10004 USA SN 1073-449X EI 1535-4970 J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR 15 PY 2013 VL 187 IS 8 BP 840 EP 847 DI 10.1164/rccm.201207-1222OC PG 8 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA 133MA UT WOS:000318141300013 PM 23348978 ER PT J AU McKinney, JS Sethi, S Tripp, JD Nguyen, TN Sanderson, BA Westmoreland, JW Resnick, MA Lewis, LK AF McKinney, Jennifer Summers Sethi, Sunaina Tripp, Jennifer DeMars Nguyen, Thuy N. Sanderson, Brian A. Westmoreland, James W. Resnick, Michael A. Lewis, L. Kevin TI A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae SO BMC GENOMICS LA English DT Article DE EcoRI; Homologous recombination; End-joining; Double-strand break; Bleomycin; MMS; Radiation; RAD52; Gene ontology (GO); Overlapping genes ID IONIZING-RADIATION; HOMOLOGOUS RECOMBINATION; BIOLOGICAL CONSEQUENCES; CHECKPOINT ACTIVATION; PATHWAY CHOICE; WIDE SCREEN; END; YEAST; DAMAGE; RESISTANCE AB Background: Efficient mechanisms for rejoining of DNA double-strand breaks (DSBs) are vital because misrepair of such lesions leads to mutation, aneuploidy and loss of cell viability. DSB repair is mediated by proteins acting in two major pathways, called homologous recombination and nonhomologous end-joining. Repair efficiency is also modulated by other processes such as sister chromatid cohesion, nucleosome remodeling and DNA damage checkpoints. The total number of genes influencing DSB repair efficiency is unknown. Results: To identify new yeast genes affecting DSB repair, genes linked to gamma radiation resistance in previous genome-wide surveys were tested for their impact on repair of site-specific DSBs generated by in vivo expression of EcoRI endonuclease. Eight members of the RAD52 group of DNA repair genes (RAD50, RAD51, RAD52, RAD54, RAD55, RAD57, MRE11 and XRS2) and 73 additional genes were found to be required for efficient repair of EcoRI-induced DSBs in screens utilizing both MATa and MATa deletion strain libraries. Most mutants were also sensitive to the clastogenic chemicals MMS and bleomycin. Several of the non-RAD52 group genes have previously been linked to DNA repair and over half of the genes affect nuclear processes. Many proteins encoded by the protective genes have previously been shown to associate physically with each other and with known DNA repair proteins in high-throughput proteomics studies. A majority of the proteins (64%) share sequence similarity with human proteins, suggesting that they serve similar functions. Conclusions: We have used a genetic screening approach to detect new genes required for efficient repair of DSBs in Saccharomyces cerevisiae. The findings have spotlighted new genes that are critical for maintenance of genome integrity and are therefore of greatest concern for their potential impact when the corresponding gene orthologs and homologs are inactivated or polymorphic in human cells. C1 [McKinney, Jennifer Summers; Sethi, Sunaina; Tripp, Jennifer DeMars; Nguyen, Thuy N.; Sanderson, Brian A.; Lewis, L. Kevin] SW Texas State Univ, Dept Chem & Biochem, San Marcos, TX 78666 USA. [Westmoreland, James W.; Resnick, Michael A.] NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA. RP Lewis, LK (reprint author), SW Texas State Univ, Dept Chem & Biochem, San Marcos, TX 78666 USA. EM LL18@txstate.edu OI Sanderson, Brian/0000-0002-3227-8299 FU National Institutes of Health [1R15GM09904901]; Welch Foundation FX The authors wish to thank Stacia Engel at the Saccharomyces Genome Database for expert advice on use of the YeastMine software. This work was supported in part by a grant from the National Institutes of Health (grant number 1R15GM09904901) to LKL and a departmental grant from the Welch Foundation. NR 55 TC 6 Z9 6 U1 0 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 15 PY 2013 VL 14 AR 251 DI 10.1186/1471-2164-14-251 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 138QB UT WOS:000318522300001 PM 23586741 ER PT J AU Teer, JK Johnston, JJ Anzick, SL Pineda, M Stone, G Meltzer, PS Mullikin, JC Biesecker, LG AF Teer, Jamie K. Johnston, Jennifer J. Anzick, Sarah L. Pineda, Marbin Stone, Gary Meltzer, Paul S. Mullikin, James C. Biesecker, Leslie G. CA NISC Comparative Sequencing Progra TI Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting SO BMC GENOMICS LA English DT Article DE Flow sorting; Flow cytometry; Targeted-sequencing; Sequencing; Genomic-capture; Chromosome; Genome ID HUMAN GENOME; BARLEY; WHEAT; TRANSLOCATION; RESOLUTION; ELEMENTS; PROJECT; COMMON AB Background: Targeted capture, combined with massively-parallel sequencing, is a powerful technique that allows investigation of specific portions of the genome for less cost than whole genome sequencing. Several methods have been developed, and improvements have resulted in commercial products targeting the human or mouse exonic regions (the exome). In some cases it is desirable to custom-target other regions of the genome, either to reduce the amount of sequence that is targeted or to capture regions that are not targeted by commercial kits. It is important to understand the advantages, limitations, and complexity of a given capture method before embarking on a targeted sequencing experiment. Results: We compared two custom targeted capture methods suitable for single chromosome analysis: Solution Hybrid Selection (SHS) and Flow Sorting (FS) of single chromosomes. Both methods can capture targeted material and result in high percentages of genotype identifications across these regions: 59-92% for SHS and 70-79% for FS. FS is amenable to current structural variation detection methods, and variants were detected. Structural variation was also assessed for SHS samples with paired end sequencing, resulting in variant identification. Conclusions: While both methods can effectively target genomic regions for genotype determination, several considerations make each method appropriate in different circumstances. SHS is well suited for experiments targeting smaller regions in a larger number of samples. FS is well suited when regions of interest cover large regions of a single chromosome. Although whole genome sequencing is becoming less expensive, the sequencing, data storage, and analysis costs make targeted sequencing using SHS or FS a compelling option. C1 [Teer, Jamie K.; Johnston, Jennifer J.; Mullikin, James C.; Biesecker, Leslie G.; NISC Comparative Sequencing Progra] NHGRI, NIH, Bethesda, MD 20892 USA. [Anzick, Sarah L.; Pineda, Marbin; Stone, Gary; Meltzer, Paul S.] NCI, NIH, Bethesda, MD 20892 USA. RP Teer, JK (reprint author), NHGRI, NIH, Bethesda, MD 20892 USA. EM Jamie.Teer@moffitt.org FU National Human Genome Research Institute; National Cancer Institute FX This study was supported by the Intramural Research Programs of the National Human Genome Research Institute and the National Cancer Institute. This study is in memory of Gary Stone, not only an excellent flow cytometrist whose skill and dedication made this and many other studies possible, but a friend and colleague who is greatly missed. NR 30 TC 2 Z9 2 U1 1 U2 16 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 15 PY 2013 VL 14 AR 253 DI 10.1186/1471-2164-14-253 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 138QB UT WOS:000318522300003 PM 23586822 ER PT J AU Lion, M Bisio, A Tebaldi, T De Sanctis, V Menendez, D Resnick, MA Ciribilli, Y Inga, A AF Lion, Mattia Bisio, Alessandra Tebaldi, Toma De Sanctis, Veronica Menendez, Daniel Resnick, Michael A. Ciribilli, Yari Inga, Alberto TI Interaction between p53 and estradiol pathways in transcriptional responses to chemotherapeutics SO CELL CYCLE LA English DT Article DE 17-beta estradiol; MCF7 cells; cis-element; doxorubicin; estrogen receptor; non-canonical response elements; nutlin; p53; synergistic cooperation; synergy ID ESTROGEN-RECEPTOR-ALPHA; BREAST-CANCER CELLS; DNA-DAMAGE RESPONSE; HISTONE DEMETHYLASES; NUCLEAR RECEPTORS; TUMOR SUPPRESSION; TARGET GENE; APOPTOSIS; EXPRESSION; ACTIVATION AB Estrogen receptors (ERs) and p53 can interact via cis-elements to regulate the angiogenesis-related VEGFR-1 (FLT1) gene, as we reported previously. Here, we address cooperation between these transcription factors on a global scale. Human breast adenocarcinoma MCF7 cells were exposed to single or combinatorial treatments with the chemotherapeutic agent doxorubicin and the ER ligand 17-estradiol (E2). Whole-genome transcriptome changes were measured by expression microarrays. Nearly 200 differentially expressed genes were identified that showed limited responsiveness to either doxorubicin treatment or ER ligand alone but were upregulated in a greater than additive manner following combined treatment. Based on exposure to 5-fuorouracil and nutlin-3a, the combined responses were treatment-specific. Among 16 genes chosen for validation using quantitative real-time PCR, seven (INPP5D, TLR5, KRT15, EPHA2, GDNF, NOTCH1, SOX9) were confirmed to be novel direct targets of p53, based on responses in MCF7 cells silenced for p53 or cooperative targets of p53 and ER. Promoter pattern searches and chromatin IP experiments for the INPP5D, TLR5, KRT15 genes supported direct, cis-mediated p53 and/or ER regulation through canonical and noncanonical p53 and ER response elements. Collectively, we establish that combinatorial activation of p53 and ER can induce novel gene expression programs that have implications for cell-cell communications, adhesion, cell differentiation, development and inflammatory responses as well as cancer treatments. C1 [Lion, Mattia; Bisio, Alessandra; De Sanctis, Veronica; Ciribilli, Yari; Inga, Alberto] Univ Trent, Ctr Integrat Biol CIBIO, Lab Transcript Networks, Mattarello, Trento, Italy. [Tebaldi, Toma] Univ Trent, Ctr Integrat Biol CIBIO, Lab Translat Genom, Mattarello, Trento, Italy. [Menendez, Daniel; Resnick, Michael A.] NIEHS, Chromosome Stabil Grp, Res Triangle Pk, NC 27709 USA. RP Inga, A (reprint author), Univ Trent, Ctr Integrat Biol CIBIO, Lab Transcript Networks, Mattarello, Trento, Italy. EM inga@science.unitn.it OI Tebaldi, Toma/0000-0002-0625-1631 FU Italian Association for Cancer Research, AIRC [IG9086]; CIBIO; NIEHS [Z01 ES065079]; Marie-Curie/Autonomous-Province-of-Trento (PAT) co-fund grant [40101712]; Pezcoller Foundation, Trento FX We thank Dr Valentina Adami for technical assistance with the microarray experiments. This work was partially supported by the Italian Association for Cancer Research, AIRC (#IG9086 to AI), by CIBIO start-up funds and by the Intramural Research Program of the NIEHS (to D. M. and M. A. R.: Z01 ES065079). M. L. is a PhD Fellow of the International Doctorate in Biomolecular Sciences, University of Trento. Y.C. is supported by a Marie-Curie/Autonomous-Province-of-Trento (PAT) co-fund grant (#40101712). V.dS. was supported by a Fellowship from Pezcoller Foundation, Trento. NR 74 TC 18 Z9 18 U1 0 U2 10 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1538-4101 J9 CELL CYCLE JI Cell Cycle PD APR 15 PY 2013 VL 12 IS 8 BP 1211 EP 1224 DI 10.4161/cc.24309 PG 14 WC Cell Biology SC Cell Biology GA 132KX UT WOS:000318067700013 PM 23518503 ER PT J AU Zhang, XY He, YL Lee, KH Dubois, W Li, ZQ Wu, XL Kovalchuk, A Zhang, WM Huang, J AF Zhang, Xinyue He, Yunlong Lee, Kyoung-Hwa Dubois, Wendy Li, Ziqing Wu, Xiaolin Kovalchuk, Alexander Zhang, Weimin Huang, Jing TI Rap2b, a novel p53 target, regulates p53-mediated pro-survival function SO CELL CYCLE LA English DT Article DE gene regulation; chromatin; p53; epigenetics ID EMBRYONIC STEM-CELLS; DNA-DAMAGE RESPONSE; TRANSCRIPTIONAL REGULATION; CYCLE ARREST; GENOME-WIDE; TUMOR-SUPPRESSOR; GENOTOXIC STRESS; G(1) ARREST; IN-VIVO; APOPTOSIS AB The tumor suppressor p53 is a critical regulator of apoptosis and cell cycle arrest/pro-survival. Upon DNA damage, p53 evokes both cell cycle arrest/pro-survival and apoptosis transcriptional programs. The ultimate cellular outcome depends on the balance of these two programs. However, the p53 downstream targets that mediate this cell fate decision remain to be identified. Using an integrative genomic approach, we identify Rap2b as a conserved p53-activated gene that counters p53-mediated apoptosis after DNA damage. Upon DNA damage, p53 directly binds to the promoter of Rap2b and activates its transcription. The reduction of Rap2b levels by small interference RNA sensitizes cells to DNA damage-induced apoptosis in a p53-dependent manner. Consistent with its pro-survival function, analysis of cancer genomic data reveals that Rap2b is overexpressed in many types of tumors. Anchorage-independent growth assays show that Rap2b has only weak transformation activity, suggesting that it is not an oncogene by itself. Together, our results identify Rap2b as a new player in the pro-survival program conducted by p53 and raise the possibility that targeting Rap2b could sensitize tumor cells to apoptosis in response to DNA damage. C1 [Zhang, Xinyue; He, Yunlong; Dubois, Wendy; Li, Ziqing; Huang, Jing] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. [Lee, Kyoung-Hwa] Seoul Natl Univ, Sch Med, Med Res Ctr, Ischem Hypox Dis Inst, Seoul, South Korea. [Wu, Xiaolin] NCI, Lab Mol Technol, Frederick, MD 21701 USA. [Kovalchuk, Alexander] NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. [Zhang, Weimin] Zhejiang Univ, Sch Med, Ctr Biomed Res, Hangzhou 310003, Zhejiang, Peoples R China. RP Huang, J (reprint author), NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. EM huangj3@mail.nih.gov RI Huang, Jing/A-2566-2009; He, Yunlong/D-1278-2017 OI Huang, Jing/0000-0002-7163-5156; FU Center for Cancer Research (CCR), National Cancer Institute; National Institutes of Health, USA; National Institute of Allergy and Infectious Diseases; National Institute of Health FX J.H.'s laboratory was funded by the intramural research program of the Center for Cancer Research (CCR), National Cancer Institute and National Institutes of Health, USA. This research was also supported in part by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases and National Institute of Health. NR 56 TC 24 Z9 25 U1 4 U2 14 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1538-4101 J9 CELL CYCLE JI Cell Cycle PD APR 15 PY 2013 VL 12 IS 8 BP 1279 EP 1291 DI 10.4161/cc.24364 PG 13 WC Cell Biology SC Cell Biology GA 132KX UT WOS:000318067700019 PM 23535297 ER PT J AU Hogue, CJR Parker, CB Willinger, M Temple, JR Bann, CM Silver, RM Dudley, DJ Koch, MA Coustan, DR Stoll, BJ Reddy, UM Varner, MW Saade, GR Conway, D Goldenberg, RL AF Hogue, Carol J. R. Parker, Corette B. Willinger, Marian Temple, Jeff R. Bann, Carla M. Silver, Robert M. Dudley, Donald J. Koch, Matthew A. Coustan, Donald R. Stoll, Barbara J. Reddy, Uma M. Varner, Michael W. Saade, George R. Conway, Deborah Goldenberg, Robert L. CA Eunice Kennedy Shriver Natl Inst Stillbirth Collaborative Res Netwo TI A Population-based Case-Control Study of Stillbirth: The Relationship of Significant Life Events to the Racial Disparity for African Americans SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE African Americans; case-control studies; continental population groups; life change events; psychosocial stress; socioeconomic factors; stillbirth; stress ID SPONTANEOUS PRETERM BIRTH; RESIDENTIAL-MOBILITY; PSYCHOLOGICAL DISTRESS; ETHNIC DISPARITIES; MATERNAL EXPOSURE; FOREIGN-BORN; RISK-FACTORS; US-BORN; PREGNANCY; STRESS AB Stillbirths (fetal deaths occurring at 20 weeks gestation) are approximately equal in number to infant deaths in the United States and are twice as likely among non-Hispanic black births as among non-Hispanic white births. The causes of racial disparity in stillbirth remain poorly understood. A population-based case-control study conducted by the Stillbirth Collaborative Research Network in 5 US catchment areas from March 2006 to September 2008 identified characteristics associated with racial/ethnic disparity and interpersonal and environmental stressors, including a list of 13 significant life events (SLEs). The adjusted odds ratio for stillbirth among women reporting all 4 SLE factors (financial, emotional, traumatic, and partner-related) was 2.22 (95 confidence interval: 1.43, 3.46). This association was robust after additional control for the correlated variables of family income, marital status, and health insurance type. There was no interaction between race/ethnicity and other variables. Effective ameliorative interventions could have a substantial public health impact, since there is at least a 50 increased risk of stillbirth for the approximately 21 of all women and 32 of non-Hispanic black women who experience 3 or more SLE factors during the year prior to delivery. C1 [Hogue, Carol J. R.] Emory Univ, Rollins Sch Publ Hlth, Dept Epidemiol, Atlanta, GA 30322 USA. [Goldenberg, Robert L.] Drexel Univ, Sch Med, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA. [Temple, Jeff R.; Saade, George R.] Univ Texas Med Branch Galveston, Dept Obstet & Gynecol, Galveston, TX USA. [Stoll, Barbara J.] Emory Univ, Sch Med, Dept Pediat, Atlanta, GA 30322 USA. [Stoll, Barbara J.] Childrens Healthcare Atlanta, Atlanta, GA USA. [Coustan, Donald R.] Brown Univ, Alpert Med Sch, Dept Obstet & Gynecol, Div Maternal Fetal Med, Providence, RI 02912 USA. [Dudley, Donald J.; Conway, Deborah] Univ Texas Hlth Sci Ctr San Antonio, Dept Obstet & Gynecol, Div Maternal Fetal Med, San Antonio, TX 78229 USA. [Silver, Robert M.; Varner, Michael W.] Univ Utah, Sch Med, Dept Obstet & Gynecol, Div Maternal Fetal Med, Salt Lake City, UT 84132 USA. [Silver, Robert M.; Varner, Michael W.] Intermt Healthcare, Maternal Fetal Med Unit, Salt Lake City, UT USA. [Willinger, Marian; Reddy, Uma M.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pregnancy & Perinatol Branch, Bethesda, MD USA. [Parker, Corette B.; Bann, Carla M.; Koch, Matthew A.] RTI Int, Stat & Epidemiol Unit, Div Hlth Sci, Res Triangle Pk, NC USA. RP Hogue, CJR (reprint author), Emory Univ, Rollins Sch Publ Hlth, Dept Epidemiol, 1518 Clifton Rd NE,1518-002-3BB, Atlanta, GA 30322 USA. EM chogue@emory.edu RI Hogue, Carol/H-5442-2012; Temple, Jeff/A-7666-2009; Varner, Michael/K-9890-2013 OI Temple, Jeff/0000-0003-3193-0510; Varner, Michael/0000-0001-9455-3973 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health; Office of Research in Women's Health, National Institutes of Health [U10-HD045953, U10-HD045925, U10-HD045952, U10-HD045955, UK10-HD045944, U10-HD045954] FX This study was supported by grant funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, with supplemental funding from the Office of Research in Women's Health, National Institutes of Health (grants U10-HD045953 (Brown University), U10-HD045925 (Emory University), U10-HD045952 (University of Texas Medical Branch at Galveston), U10-HD045955 (University of Texas Health Science Center at San Antonio), UK10-HD045944 (University of Utah Health Sciences Center), and U10-HD045954 (RTI International)). NR 52 TC 14 Z9 15 U1 4 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD APR 15 PY 2013 VL 177 IS 8 BP 755 EP 767 DI 10.1093/aje/kws381 PG 13 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 124AT UT WOS:000317435600005 PM 23531847 ER PT J AU Hofmann, JN Moore, SC Lim, U Park, Y Baris, D Hollenbeck, AR Matthews, CE Gibson, TM Hartge, P Purdue, MP AF Hofmann, Jonathan N. Moore, Steven C. Lim, Unhee Park, Yikyung Baris, Dalsu Hollenbeck, Albert R. Matthews, Charles E. Gibson, Todd M. Hartge, Patricia Purdue, Mark P. TI Body Mass Index and Physical Activity at Different Ages and Risk of Multiple Myeloma in the NIH-AARP Diet and Health Study SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE body mass index; multiple myeloma; obesity; overweight; physical activity ID ANTHROPOMETRIC CHARACTERISTICS; LYMPHOHEMATOPOIETIC MALIGNANCIES; TRANSPLANT RECIPIENTS; ADIPOSE-TISSUE; GROWTH-FACTOR; CANCER; PROLIFERATION; ASSOCIATION; LYMPHOMA; SURVIVAL AB Several studies have reported an increased risk of multiple myeloma associated with excess body weight. We investigated the risk of multiple myeloma in relation to separate measures of adiposity and energy balance at different ages in the National Institutes of Health-AARP Diet and Health Study, a large prospective cohort study in the United States. Participants completed a baseline questionnaire (19951996; n 485,049), and a subset of participants completed a second questionnaire (19961997; n 305,618) in which we solicited more detailed exposure information. Hazard ratios and 95 confidence intervals were estimated for the risk of multiple myeloma (overall, n 813; subset, n 489) in relation to several measures of obesity and leisure time physical activity. Multiple myeloma risk was associated with increasing body mass index (BMI) at cohort entry (per 5-kg/m(2) increase, hazard ratio (HR) 1.10, 95 confidence interval (CI): 1.00, 1.22); similar associations were observed for BMI at age 50 years (HR 1.14, 95 CI: 1.02, 1.28), age 35 years (HR 1.20, 95 CI: 1.05, 1.36), and age 18 years (HR 1.13, 95 CI: 0.98, 1.32) without adjustment for baseline BMI. Risk of multiple myeloma was not associated with physical activity level at any age. These findings support the hypothesis that excess body weight, both in early adulthood and later in life, is a risk factor for multiple myeloma and suggest that maintaining a healthy body weight throughout life may reduce multiple myeloma risk. C1 [Hofmann, Jonathan N.; Moore, Steven C.; Park, Yikyung; Baris, Dalsu; Matthews, Charles E.; Gibson, Todd M.; Hartge, Patricia; Purdue, Mark P.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Lim, Unhee] Univ Hawaii, Ctr Canc, Canc Epidemiol Program, Honolulu, HI 96822 USA. [Hollenbeck, Albert R.] AARP, Washington, DC USA. RP Hofmann, JN (reprint author), NCI, Occupat & Environm Epidemiol Branch, Div Canc Epidemiol & Genet, 6120 Execut Blvd,EPS 8109, Bethesda, MD 20892 USA. EM hofmannjn@mail.nih.gov RI matthews, Charles/E-8073-2015; Purdue, Mark/C-9228-2016; Moore, Steven/D-8760-2016; OI matthews, Charles/0000-0001-8037-3103; Purdue, Mark/0000-0003-1177-3108; Moore, Steven/0000-0002-8169-1661; Park, Yikyung/0000-0002-6281-489X FU Intramural Research Program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics. NR 31 TC 11 Z9 11 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD APR 15 PY 2013 VL 177 IS 8 BP 776 EP 786 DI 10.1093/aje/kws295 PG 11 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 124AT UT WOS:000317435600007 PM 23543160 ER PT J AU Neta, G Rajaraman, P de Gonzalez, AB Doody, MM Alexander, BH Preston, D Simon, SL Melo, D Miller, J Freedman, DM Linet, MS Sigurdson, AJ AF Neta, Gila Rajaraman, Preetha de Gonzalez, Amy Berrington Doody, Michele M. Alexander, Bruce H. Preston, Dale Simon, Steven L. Melo, Dunstana Miller, Jeremy Freedman, D. Michal Linet, Martha S. Sigurdson, Alice J. TI A Prospective Study of Medical Diagnostic Radiography and Risk of Thyroid Cancer SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE radiation; radiography; thyroid gland; thyroid neoplasms; x-rays ID DENTAL X-RAYS; US RADIOLOGIC TECHNOLOGISTS; POOLED ANALYSIS; RADIATION; EXPOSURE; WOMEN; FEMALE AB Although diagnostic x-ray procedures provide important medical benefits, cancer risks associated with their exposure are also possible, but not well characterized. The US Radiologic Technologists Study (19832006) is a nationwide, prospective cohort study with extensive questionnaire data on history of personal diagnostic imaging procedures collected prior to cancer diagnosis. We used Cox proportional hazard regressions to estimate thyroid cancer risks related to the number and type of selected procedures. We assessed potential modifying effects of age and calendar year of the first x-ray procedure in each category of procedures. Incident thyroid cancers (n 251) were diagnosed among 75,494 technologists (1.3 million person-years; mean follow-up 17 years). Overall, there was no clear evidence of thyroid cancer risk associated with diagnostic x-rays except for dental x-rays. We observed a 13 increase in thyroid cancer risk for every 10 reported dental radiographs (hazard ratio 1.13, 95 confidence interval: 1.01, 1.26), which was driven by dental x-rays first received before 1970, but we found no evidence that the relationship between dental x-rays and thyroid cancer was associated with childhood or adolescent exposures as would have been anticipated. The lack of association of thyroid cancer with x-ray procedures that expose the thyroid to higher radiation doses than do dental x-rays underscores the need to conduct a detailed radiation exposure assessment to enable quantitative evaluation of risk. C1 [Neta, Gila; Rajaraman, Preetha; de Gonzalez, Amy Berrington; Doody, Michele M.; Simon, Steven L.; Melo, Dunstana; Freedman, D. Michal; Linet, Martha S.; Sigurdson, Alice J.] NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,Dept Hlth & Human Serv, Rockville, MD USA. [Preston, Dale] HiroSoft Int Corp, Seattle, WA USA. [Alexander, Bruce H.] Univ Minnesota, Dept Environm Hlth Sci, Minneapolis, MN USA. [Melo, Dunstana] Inst Radioprotecao & Dosimetria, Rio De Janeiro, Brazil. [Miller, Jeremy] Informat Management Syst, Silver Spring, MD USA. RP Neta, G (reprint author), NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, 6120 Execut Blvd,EPS 7049,MSC 7238, Bethesda, MD 20892 USA. EM netagil@mail.nih.gov FU Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health FX This research was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health. NR 33 TC 10 Z9 10 U1 1 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD APR 15 PY 2013 VL 177 IS 8 BP 800 EP 809 DI 10.1093/aje/kws315 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 124AT UT WOS:000317435600009 PM 23529772 ER PT J AU Petrini, I Wang, YS Zucali, PA Lee, HS Pham, T Voeller, D Meltzer, PS Giaccone, G AF Petrini, Iacopo Wang, Yisong Zucali, Paolo A. Lee, Hye Seung Trung Pham Voeller, Donna Meltzer, Paul S. Giaccone, Giuseppe TI Copy Number Aberrations of Genes Regulating Normal Thymus Development in Thymic Epithelial Tumors SO CLINICAL CANCER RESEARCH LA English DT Article ID BREAST-CANCER; DNA METHYLATION; LUNG ADENOCARCINOMA; DRUG-SENSITIVITY; CELL-GROWTH; ORGANOGENESIS; EXPRESSION; CARCINOMA; SURVIVAL; FOXC1 AB Purposes: To determine whether the deregulation of genes relevant for normal thymus development can contribute to the biology of thymic epithelial tumors (TET). Experimental Design: Using array comparative genomic hybridization, we evaluated the copy number aberrations of genes regulating thymus development. The expression of genes most commonly involved in copy number aberrations was evaluated by immunohistochemistry and correlated with patients' outcome. Correlation between FOXC1 copy number loss and gene expression was determined in a confirmation cohort. Cell lines were used to test the role of FOXC1 in tumors. Results: Among 31 thymus development-related genes, PBX1 copy number gain and FOXC1 copy number loss were presented in 43.0% and 39.5% of the tumors, respectively. Immunohistochemistry on a series of 132 TETs, including those evaluated by comparative genomic hybridization, revealed a correlation between protein expression and copy number status only for FOXC1 but not for PBX1. Patients with FOXC1 negative tumors had a shorter time to progression and a trend for a shorter disease-related survival. The correlation between FOXC1 copy number loss and mRNA expression was confirmed in a separate cohort of 27 TETs. Ectopic FOXC1 expression attenuated anchorage-independent cell growth and cell migration in vitro. Conclusion: Our data support a tumor suppressor role of FOXC1 in TETs. Clin Cancer Res; 19(8); 1960-71. (C) 2013 AACR. C1 [Petrini, Iacopo; Wang, Yisong; Lee, Hye Seung; Trung Pham; Voeller, Donna; Giaccone, Giuseppe] NCI, Med Oncol Branch, NIH, Bethesda, MD 20892 USA. [Meltzer, Paul S.] NCI, Genet Branch, NIH, Bethesda, MD 20892 USA. [Zucali, Paolo A.] IRCCS, Humanitas Canc Ctr, Dept Med Oncol, Milan, Italy. [Lee, Hye Seung] Seoul Natl Univ, Bundang Hosp, Dept Pathol, Songnam, Gyeonggi, South Korea. RP Giaccone, G (reprint author), NCI, Med Oncol Branch, 10 Ctr Dr, Bethesda, MD 20892 USA. EM giacconeg@mail.nih.gov RI Lee, Hye Seung/G-6419-2011; Petrini, Iacopo/K-7316-2016; Giaccone, Giuseppe/E-8297-2017 OI Lee, Hye Seung/0000-0002-1667-7986; Petrini, Iacopo/0000-0002-7752-6866; Giaccone, Giuseppe/0000-0002-5023-7562 FU NCI intramural program FX This work was supported by the NCI intramural program. NR 39 TC 8 Z9 9 U1 1 U2 7 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 J9 CLIN CANCER RES JI Clin. Cancer Res. PD APR 15 PY 2013 VL 19 IS 8 BP 1960 EP 1971 DI 10.1158/1078-0432.CCR-12-3260 PG 12 WC Oncology SC Oncology GA 126FG UT WOS:000317597500007 PM 23444221 ER PT J AU Carpenter, RO Evbuomwan, MO Pittaluga, S Rose, JJ Raffeld, M Yang, SC Gress, RE Hakim, FT Kochenderfer, JN AF Carpenter, Robert O. Evbuomwan, Moses O. Pittaluga, Stefania Rose, Jeremy J. Raffeld, Mark Yang, Shicheng Gress, Ronald E. Hakim, Frances T. Kochenderfer, James N. TI B-cell Maturation Antigen Is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma SO CLINICAL CANCER RESEARCH LA English DT Article ID ACTIVATING FACTOR; ANTITUMOR-ACTIVITY; PLASMA-CELLS; RECEPTOR; BAFF; BCMA; SURVIVAL; LYMPHOCYTES; APRIL; EXPRESSION AB Purpose: Multiple myeloma is a usually incurable malignancy of plasma cells. New therapies are urgently needed for multiple myeloma. Adoptive transfer of chimeric antigen receptor (CAR)-expressing T cells is a promising new therapy for hematologic malignancies, but an ideal target antigen for CAR-expressing T-cell therapies for multiple myeloma has not been identified. B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells. Our goal was to determine if BCMA is a suitable target for CAR-expressing T cells. Experimental Design: We conducted an assessment of BCMA expression in normal human tissues and multiple myeloma cells by flow cytometry, quantitative PCR, and immunohistochemistry. We designed and tested novel anti-BCMA CARs. Results: BCMA had a restricted RNA expression pattern. Except for expression in plasma cells, BCMA protein was not detected in normal human tissues. BCMA was not detected on primary human CD34(+) hematopoietic cells. We detected uniform BCMA cell-surface expression on primary multiple myeloma cells from five of five patients. We designed the first anti-BCMA CARs to be reported and we transduced T cells with lentiviral vectors encoding these CARs. The CARs gave T cells the ability to specifically recognize BCMA. The anti-BCMA-CAR-transduced T cells exhibited BCMA-specific functions including cytokine production, proliferation, cytotoxicity, and in vivo tumor eradication. Importantly, anti-BCMA-CAR-transduced T cells recognized and killed primary multiple myeloma cells. Conclusions: BCMA is a suitable target for CAR-expressing T cells, and adoptive transfer of anti-BCMA-CAR-expressing T cells is a promising new strategy for treating multiple myeloma. Clin Cancer Res; 19(8); 2048-60. (C) 2013 AACR. C1 [Carpenter, Robert O.; Rose, Jeremy J.; Gress, Ronald E.; Hakim, Frances T.; Kochenderfer, James N.] NCI, Expt Transplantat & Immunol Branch, Bethesda, MD 20892 USA. [Evbuomwan, Moses O.; Pittaluga, Stefania; Raffeld, Mark] NCI, Pathol Lab, Bethesda, MD 20892 USA. [Yang, Shicheng] Duke Univ, Dept Surg, Sch Med, Div Neurosurg, Durham, NC USA. RP Kochenderfer, JN (reprint author), NCI, NIH, 10 Ctr Dr CRC,Room 3-3330, Bethesda, MD 20892 USA. EM kochendj@mail.nih.gov FU Center for Cancer Research, National Cancer Institute, NIH FX This work was supported by intramural funding of the Center for Cancer Research, National Cancer Institute, NIH. NR 50 TC 81 Z9 87 U1 2 U2 12 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 J9 CLIN CANCER RES JI Clin. Cancer Res. PD APR 15 PY 2013 VL 19 IS 8 BP 2048 EP 2060 DI 10.1158/1078-0432.CCR-12-2422 PG 13 WC Oncology SC Oncology GA 126FG UT WOS:000317597500015 PM 23344265 ER PT J AU Mattison, HA Nie, H Gao, HM Zhou, H Hong, JS Zhang, J AF Mattison, Hayley A. Nie, Hui Gao, Huiming Zhou, Hui Hong, Jau-Shyong Zhang, Jing TI Suppressed pro-inflammatory response of microglia in CX3CR1 knockout mice SO JOURNAL OF NEUROIMMUNOLOGY LA English DT Article DE Parkinson's disease; Alzheimer's disease; Stroke; Microglia; Fractalkine; Chemokines ID CENTRAL-NERVOUS-SYSTEM; FRACTALKINE-RECEPTOR; DOPAMINERGIC-NEURONS; AMYLOID DEPOSITION; ALZHEIMERS-DISEASE; ALPHA-SYNUCLEIN; MOUSE; ACTIVATION; CHEMOKINE; BRAIN AB Neuronal fractalkine acts via its receptor, CX3CR1, on microglia to regulate neuroinflammation. Conflicting results have been reported in studies employing CX3CR1 deficient (Cx3cr1(-/-)) mice. Here, compared to wild-type, endotoxin-treated neuron-glial Cx3cr1(-/-)cultures produced less TNF-alpha, nitric oxide and superoxide; however, fractalkine treatment inhibited the release of pro-inflammatory factors in wild-type and BV-2 cell cultures. Furthermore, endotoxin-treated BV-2 cells expressing siRNA against CX3CR1 increased nitric oxide and TNF-alpha production. We hypothesize that CX3CL1-CX3CR1 signaling is neuroprotective and propose that the reduced production of pro-inflammatory signals in Cx3cr1(-/-)microglia may result from compensatory mechanisms and not be the direct result of CX3CR1 deficiency. (C) 2013 Elsevier B.V. All rights reserved. C1 [Mattison, Hayley A.; Zhang, Jing] Univ Washington, Dept Pathol, Seattle, WA 98104 USA. [Nie, Hui; Gao, Huiming; Zhou, Hui; Hong, Jau-Shyong] NIEHS, Neuropharmacol Sect, Pharmacol Lab, Res Triangle Pk, NC 27709 USA. RP Zhang, J (reprint author), Univ Washington, Dept Pathol, Sch Med, Box 359635,325 9th Ave, Seattle, WA 98104 USA. EM zhangj@uw.edu FU NIEHS; NIH [ES016873, ES019277, N5057567, NS062684-(6221), ES007033 (6364), ES004696 (5897), AG033398, NS082137] FX This research was supported by intramural program of NIEHS (Dr. IS Hong) as well as NIH grants to Dr. J Zhang [ES016873, ES019277; N5057567; NS062684-(6221), ES007033 (6364), ES004696 (5897), AG033398, and NS082137]. NR 27 TC 16 Z9 16 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-5728 J9 J NEUROIMMUNOL JI J. Neuroimmunol. PD APR 15 PY 2013 VL 257 IS 1-2 BP 110 EP 115 DI 10.1016/j.jneuroim.2013.02.008 PG 6 WC Immunology; Neurosciences SC Immunology; Neurosciences & Neurology GA 131MY UT WOS:000317999000017 PM 23499256 ER PT J AU Tiso, M Schechter, AN AF Tiso, Mauro Schechter, Alan N. TI Enteric bacterial nitrate/nitrite/nitric oxide/ammonia metabolism SO NITRIC OXIDE-BIOLOGY AND CHEMISTRY LA English DT Meeting Abstract CT 5th Bi-Annual International Meeting on the Role of Nitrite and Nitrate in Physiology, Pathophysiology, and Therapeutics CY MAY 04-05, 2013 CL Pittsburgh, PA SP Univ Pittsburgh, Vasc Med Inst, Univ Pittsburgh, Dept Surg, Univ Pittsburgh, Dept Pharmacol, Wake Forest Univ, Karolinska Inst, Heinrich Heine Univ Dusseldorf C1 [Tiso, Mauro; Schechter, Alan N.] NIDDK, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1089-8603 J9 NITRIC OXIDE-BIOL CH JI Nitric Oxide-Biol. Chem. PD APR 15 PY 2013 VL 31 SU 1 MA P59 BP S38 EP S38 DI 10.1016/j.niox.2013.02.061 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 135CK UT WOS:000318263400060 ER PT J AU Hartley, SW Sebastiani, P AF Hartley, Stephen W. Sebastiani, Paola TI PleioGRiP: genetic risk prediction with pleiotropy SO BIOINFORMATICS LA English DT Article AB Motivation: Although several studies have used Bayesian classifiers for risk prediction using genome-wide single nucleotide polymorphism (SNP) datasets, no software can efficiently perform these analyses on massive genetic datasets and can accommodate multiple traits. Results: We describe the program PleioGRiP that performs a genome-wide Bayesian model search to identify SNPs associated with a discrete phenotype and uses SNPs ranked by Bayes factor to produce nested Bayesian classifiers. These classifiers can be used for genetic risk prediction, either selecting the classifier with optimal number of features or using an ensemble of classifiers. In addition, PleioGRiP implements an extension to the Bayesian search and classification and can search for pleiotropic relationships in which SNPs are simultaneosly associated with two or more distinct phenotypes. These relationships can be used to generate connected Bayesian classifiers to predict the phenotype of interest either using genetic data alone or in combination with the secondary phenotype(s). C1 [Hartley, Stephen W.] NHGRI, NIH, Rockville, MD 20850 USA. [Sebastiani, Paola] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA. RP Hartley, SW (reprint author), NHGRI, NIH, 5625 Fishers Lane,Suite 5N-01, Rockville, MD 20850 USA. EM stephen.hartley@nih.gov OI sebastiani, paola/0000-0001-6419-1545 FU NIH/NHLBI [R21HL114237]; NIH/NIA [U19AG023122] FX Funding: NIH/NHLBI R21HL114237 and NIH/NIA U19AG023122. NR 6 TC 3 Z9 3 U1 0 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1367-4803 J9 BIOINFORMATICS JI Bioinformatics PD APR 15 PY 2013 VL 29 IS 8 BP 1086 EP 1088 DI 10.1093/bioinformatics/btt081 PG 3 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Computer Science; Mathematical & Computational Biology; Mathematics GA 133AH UT WOS:000318109300020 PM 23419378 ER PT J AU Peng, B Chen, HS Mechanic, LE Racine, B Clarke, J Clarke, L Gillanders, E Feuer, EJ AF Peng, Bo Chen, Huann-Sheng Mechanic, Leah E. Racine, Ben Clarke, John Clarke, Lauren Gillanders, Elizabeth Feuer, Eric J. TI Genetic Simulation Resources: a website for the registration and discovery of genetic data simulators SO BIOINFORMATICS LA English DT Article ID POPULATION AB Many simulation methods and programs have been developed to simulate genetic data of the human genome. These data have been widely used, for example, to predict properties of populations retrospectively or prospectively according to mathematically intractable genetic models, and to assist the validation, statistical inference and power analysis of a variety of statistical models. However, owing to the differences in type of genetic data of interest, simulation methods, evolutionary features, input and output formats, terminologies and assumptions for different applications, choosing the right tool for a particular study can be a resource-intensive process that usually involves searching, downloading and testing many different simulation programs. Genetic Simulation Resources (GSR) is a website provided by the National Cancer Institute (NCI) that aims to help researchers compare and choose the appropriate simulation tools for their studies. This website allows authors of simulation software to register their applications and describe them with well-defined attributes, thus allowing site users to search and compare simulators according to specified features. C1 [Peng, Bo] Univ Texas MD Anderson Canc Ctr, Dept Genet, Houston, TX 77030 USA. [Chen, Huann-Sheng; Feuer, Eric J.] NCI, Stat Methodol & Applicat Branch, Surveillance Res Program, Div Canc Control & Populat Sci,NIH, Bethesda, MD 20892 USA. [Mechanic, Leah E.; Gillanders, Elizabeth] NCI, Host Susceptibil Factors Branch, Epidemiol & Genom Res Program, Div Canc Control & Populat Sci,NIH, Bethesda, MD 20892 USA. [Racine, Ben; Clarke, John; Clarke, Lauren] Cornerstone Syst Northwest Inc, Lynden, WA 98264 USA. RP Peng, B (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Genet, Houston, TX 77030 USA. EM gsr@mail.nih.gov FU National Cancer Institute [HHSN261201100558P] FX Funding: The development of GSR is supported by a contract HHSN261201100558P from the National Cancer Institute. NR 7 TC 10 Z9 10 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1367-4803 J9 BIOINFORMATICS JI Bioinformatics PD APR 15 PY 2013 VL 29 IS 8 BP 1101 EP 1102 DI 10.1093/bioinformatics/btt094 PG 2 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Computer Science; Mathematical & Computational Biology; Mathematics GA 133AH UT WOS:000318109300025 PM 23435068 ER PT J AU Lamy, L Ngo, VN Emre, NCT Shaffer, AL Yang, YD Tian, EM Nair, V Kruhlak, MJ Zingone, A Landgren, O Staudt, LM AF Lamy, Laurence Ngo, Vu N. Emre, N. C. Tolga Shaffer, Arthur L., III Yang, Yandan Tian, Erming Nair, Vinod Kruhlak, Michael J. Zingone, Adriana Landgren, Ola Staudt, Louis M. TI Control of Autophagic Cell Death by Caspase-10 in Multiple Myeloma SO CANCER CELL LA English DT Article ID LYMPHOCYTE-ACTIVATION; PLASMA-CELLS; PROTEINS; PATHOGENESIS; DEGRADATION; METABOLISM; ADDICTION; THERAPY; SCREEN; SYSTEM AB We performed a loss-of-function RNA interference screen to define therapeutic targets in multiple nnyeloma, a genetically diverse plasma cell malignancy. Unexpectedly, we discovered that all myeloma lines require caspase-10 for survival irrespective of their genetic abnormalities. The transcription factor IRF4 induces both caspase-10 and its associated protein cFLIP(L) in nnyeloma, generating a protease that does not induce apoptosis but rather blocks an autophagy-dependent cell death pathway. Caspase-10 inhibits autophagy by cleaving the BCL2-interacting protein BCLAF1, itself a strong inducer of autophagy that acts by displacing beclin-1 from BCL2. While myelorna cells require a basal level of autophagy for survival, caspase-10 tempers this response to avoid cell death. Drugs that disrupt this vital balance may have therapeutic potential in myeloma. C1 [Lamy, Laurence; Ngo, Vu N.; Emre, N. C. Tolga; Shaffer, Arthur L., III; Yang, Yandan; Zingone, Adriana; Landgren, Ola; Staudt, Louis M.] NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Kruhlak, Michael J.] NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. [Tian, Erming] Univ Arkansas Med Sci, Little Rock, AR 72205 USA. [Nair, Vinod] NIAID, Res Technol Sect, RTB, Rocky Mt Labs,NIH, Hamilton, MT 59840 USA. RP Staudt, LM (reprint author), NCI, Metab Branch, Ctr Canc Res, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM lstaudt@mail.nih.gov FU National Institutes of Health, National Cancer Institute; Center for Cancer Research FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, and the Center for Cancer Research. The authors thank Mike Lenardo for helpful discussions and Kathleen Meyer for her assistance with GEO submissions. NR 40 TC 69 Z9 71 U1 1 U2 22 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1535-6108 J9 CANCER CELL JI Cancer Cell PD APR 15 PY 2013 VL 23 IS 4 BP 435 EP 449 DI 10.1016/j.ccr.2013.02.017 PG 15 WC Oncology; Cell Biology SC Oncology; Cell Biology GA 130TV UT WOS:000317943900005 PM 23541952 ER PT J AU Jeong, SM Xiao, CY Finley, LWS Lahusen, T Souza, AL Pierce, K Li, YH Wang, XX Laurent, G German, NJ Xu, XL Li, CL Wang, RH Lee, J Csibi, A Cerione, R Blenis, J Clish, CB Kimmelman, A Deng, CX Haigis, MC AF Jeong, Seung Min Xiao, Cuiying Finley, Lydia W. S. Lahusen, Tyler Souza, Amanda L. Pierce, Kerry Li, Ying-Hua Wang, Xiaoxu Laurent, Gaelle German, Natalie J. Xu, Xiaoling Li, Cuiling Wang, Rui-Hong Lee, Jaewon Csibi, Alfredo Cerione, Richard Blenis, John Clish, Clary B. Kimmelman, Alec Deng, Chu-Xia Haigis, Marcia C. TI SIRT4 Has Tumor-Suppressive Activity and Regulates the Cellular Metabolic Response to DNA Damage by Inhibiting Mitochondrial Glutamine Metabolism SO CANCER CELL LA English DT Article ID FATTY-ACID OXIDATION; GENOMIC INSTABILITY; CALORIE RESTRICTION; INSULIN-SECRETION; CYCLE CHECKPOINT; UREA CYCLE; CANCER; CELLS; SIRTUINS; PROMOTES AB DNA damage elicits a cellular signaling response that initiates cell cycle arrest and DNA repair. Here, we find that DNA damage triggers a critical block in glutamine metabolism, which is required for proper DNA damage responses. This block requires the mitochondrial SIRT4, which is induced by numerous genotoxic agents and represses the metabolism of glutamine into tricarboxylic acid cycle. SIRT4 loss leads to both increased glutamine-dependent proliferation and stress-induced genomic instability, resulting in tumorigenic phenotypes. Moreover, SIRT4 knockout mice spontaneously develop lung tumors. Our data uncover SIRT4 as an important component of the DNA damage response pathway that orchestrates a metabolic block in glutamine metabolism, cell cycle arrest, and tumor suppression. C1 [Jeong, Seung Min; Finley, Lydia W. S.; Laurent, Gaelle; German, Natalie J.; Lee, Jaewon; Csibi, Alfredo; Blenis, John; Haigis, Marcia C.] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Cell Biol, Boston, MA 02115 USA. [Li, Ying-Hua; Wang, Xiaoxu; Kimmelman, Alec] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Radiat Oncol,Div Genom Stabil & DNA Repair, Boston, MA 02115 USA. [Xiao, Cuiying; Lahusen, Tyler; Xu, Xiaoling; Li, Cuiling; Wang, Rui-Hong; Deng, Chu-Xia] NIDDKD, Mammalian Genet Sect, Genet Dev & Dis Branch, NIH, Bethesda, MD 20892 USA. [Souza, Amanda L.; Pierce, Kerry; Clish, Clary B.] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Cerione, Richard] Cornell Univ, Dept Mol Med, Ithaca, NY 14853 USA. RP Deng, CX (reprint author), NIDDKD, Mammalian Genet Sect, Genet Dev & Dis Branch, NIH, Bethesda, MD 20892 USA. EM chuxiad@bdg10.niddk.nih.gov; marcia_haigis@hms.harvard.edu RI deng, chuxia/N-6713-2016 FU National Research Foundation of Korea; Korean Government [NRF-2010-357-C00087]; NIH [AG032375]; Glenn Foundation for Medical Research; American Cancer Society; National Cancer Institute [R01 CA157490]; Kimmel Scholar Award; AACR-PanCAN Career Development Award; Intramural Research Program of the National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, USA FX We thank Roderick T. Bronson for analyzing tumors, Moon Hee Yang for help with allograft assays, Annie Lee for technical assistance, and the Nikon Imaging Center at Harvard Medical School. We thank Kevin Haigis for critical reading of the manuscript. S.M.J. was supported in part by a National Research Foundation of Korea grant funded by the Korean Government (NRF-2010-357-C00087). M.C.H. is supported in part by NIH grant AG032375, the Glenn Foundation for Medical Research, and the American Cancer Society New Scholar Award. A.K. is supported by the National Cancer Institute grant R01 CA157490, the Kimmel Scholar Award, and an AACR-PanCAN Career Development Award. This work was also supported in part by the Intramural Research Program of the National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, USA. NR 52 TC 114 Z9 122 U1 2 U2 41 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1535-6108 J9 CANCER CELL JI Cancer Cell PD APR 15 PY 2013 VL 23 IS 4 BP 450 EP 463 DI 10.1016/j.ccr.2013.02.024 PG 14 WC Oncology; Cell Biology SC Oncology; Cell Biology GA 130TV UT WOS:000317943900006 PM 23562301 ER PT J AU Xiao, ZX Jiang, Q Willette-Brown, J Xi, SC Zhu, F Burkett, S Back, T Song, NY Datla, M Sun, ZH Goldszmid, R Lin, FC Cohoon, T Pike, K Wu, XL Schrump, DS Wong, KK Young, HA Trinchieri, G Wiltrout, RH Hu, YL AF Xiao, Zuoxiang Jiang, Qun Willette-Brown, Jami Xi, Sichuan Zhu, Feng Burkett, Sandra Back, Timothy Song, Na-Young Datla, Mahesh Sun, Zhonghe Goldszmid, Romina Lin, Fanching Cohoon, Travis Pike, Kristen Wu, Xiaolin Schrump, David S. Wong, Kwok-Kin Young, Howard A. Trinchieri, Giorgio Wiltrout, Robert H. Hu, Yinling TI The Pivotal Role of IKK alpha in the Development of Spontaneous Lung Squamous Cell Carcinomas SO CANCER CELL LA English DT Article ID KAPPA-B-KINASE; NITRIC-OXIDE SYNTHASE; STEM-CELLS; CANCER; MICE; TRANSFORMATION; SUPPRESSOR; SKIN; P63; DIFFERENTIATION AB Here, we report that kinase-dead IKK alpha knockin mice develop spontaneous lung squamous cell carcinomas (SCCs) associated with IKK alpha downregulation and marked pulmonary inflammation. IKK alpha reduction upregulated the expression of p63, Trim29, and keratin 5 (K5), which serve as diagnostic markers for human lung SCCs. IKK alpha(low)K5(+)p63(hi) cell expansion and SCC formation were accompanied by inflammation-associated deregulation of oncogenes, tumor suppressors, and stem cell regulators. Reintroducing transgenic K5.IKK alpha, depleting macrophages, and reconstituting irradiated mutant animals with wild-type bone marrow (BM) prevented SCC development, suggesting that BM-derived IKKa mutant macrophages promote the transition of I IKK alpha(low)K5(+)p63(hi) to tumor cells. This mouse model resembles human lung SCCs, sheds light on the mechanisms underlying lung malignancy development, and identifies targets for therapy of lung SCCs. C1 [Xiao, Zuoxiang; Jiang, Qun; Willette-Brown, Jami; Zhu, Feng; Back, Timothy; Song, Na-Young; Datla, Mahesh; Goldszmid, Romina; Lin, Fanching; Young, Howard A.; Trinchieri, Giorgio; Wiltrout, Robert H.; Hu, Yinling] NCI, Ctr Canc Res, Canc & Inflammat Program, Frederick, MD 21701 USA. [Xi, Sichuan; Schrump, David S.] NCI, Ctr Canc Res, Thorac Oncol Sect, Surg Branch, Frederick, MD 21701 USA. [Burkett, Sandra] NCI, Ctr Canc Res, Mouse Canc Genet Program, Frederick, MD 21701 USA. [Sun, Zhonghe; Pike, Kristen; Wu, Xiaolin] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Lab Mol Technol, Frederick, MD 21702 USA. [Cohoon, Travis; Wong, Kwok-Kin] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA. RP Wiltrout, RH (reprint author), NCI, Ctr Canc Res, Canc & Inflammat Program, Frederick, MD 21701 USA. EM wiltrour@mail.nih.gov; huy2@mail.nih.gov RI Jiang, Qun/A-1358-2014; OI wong, kwok kin/0000-0001-6323-235X FU National Cancer Institute [ZIA BC 011212, ZIA BC 011391] FX We thank Teri Plona, Scott Coccodrilli, Arati Raziuddin, Hue Vuong, Vika Grinberg, and Robin Stewart from Laboratory of Molecular Technology, SAIC-Frederick, Inc., Frederick National Laboratory for Cancer Research for sequencing DDR2, Sox2, and PIK3CA genes in mouse lung SCCs and Dr. Hesed Padilla-Nash and Dr. Thomas Ried from National Institutes of Health for kindly providing a spontaneously transformed lung murine epithelial cell line M2C. This work was supported by the National Cancer Institute (ZIA BC 011212 and ZIA BC 011391 to Y.H.). NR 43 TC 40 Z9 42 U1 0 U2 13 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1535-6108 J9 CANCER CELL JI Cancer Cell PD APR 15 PY 2013 VL 23 IS 4 BP 527 EP 540 DI 10.1016/j.ccr.2013.03.009 PG 14 WC Oncology; Cell Biology SC Oncology; Cell Biology GA 130TV UT WOS:000317943900012 PM 23597566 ER PT J AU Puigbo, P Wolf, YI Koonin, EV AF Puigbo, Pere Wolf, Yuri I. Koonin, Eugene V. TI Seeing the Tree of Life behind the phylogenetic forest SO BMC BIOLOGY LA English DT Editorial Material ID PROKARYOTIC EVOLUTION; GENE-TRANSFER; CLASSIFICATION; BACTERIA; GENOMICS; SUPPORT; ARCHAEA; SEARCH C1 [Puigbo, Pere; Wolf, Yuri I.; Koonin, Eugene V.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. RP Koonin, EV (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. EM koonin@ncbi.nlm.nih.gov NR 32 TC 15 Z9 16 U1 2 U2 45 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1741-7007 J9 BMC BIOL JI BMC Biol. PD APR 15 PY 2013 VL 11 AR 46 DI 10.1186/1741-7007-11-46 PG 3 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 127IU UT WOS:000317692700015 PM 23587361 ER PT J AU Sinicrope, FA Foster, NR Yothers, G Benson, A Seitz, JF Labianca, R Goldberg, RM DeGramont, A O'Connell, MJ Sargent, DJ AF Sinicrope, Frank A. Foster, Nathan R. Yothers, Greg Benson, Al Seitz, Jean Francois Labianca, Roberto Goldberg, Richard M. DeGramont, Aimery O'Connell, Michael J. Sargent, Daniel J. CA Adjuvant Colon Canc Endpoints TI Body mass index at diagnosis and survival among colon cancer patients enrolled in clinical trials of adjuvant chemotherapy SO CANCER LA English DT Article DE body mass index; obesity; colon cancer; adjuvant therapy; BMI ID COLORECTAL-CANCER; POSTMENOPAUSAL WOMEN; RECTAL-CANCER; WEIGHT-LOSS; US ADULTS; OBESITY; RISK; MORTALITY; OVERWEIGHT; METAANALYSIS AB BACKGROUND: Although obesity is an established risk factor for developing colon cancer, its prognostic impact and relation to patient sex in colon cancer survivors remains unclear. METHODS: The authors examined the prognostic and predictive impact of the body mass index (BMI) in patients with stage II and III colon carcinoma (N = 25,291) within the Adjuvant Colon Cancer Endpoints (ACCENT) database. BMI was measured at enrollment in randomized trials of 5-fluorouracilbased adjuvant chemotherapy. Association of BMI with the time to recurrence (TTR), disease-free survival (DFS), and overall survival (OS) were determined using Cox regression models. Statistical tests were 2-sided. RESULTS: During a median follow-up of 7.8 years, obese and underweight patients had significantly poorer survival compared with overweight and normal-weight patients. In a multivariable analysis, the adverse prognostic impact of BMI was observed among men but not among women (Pinteraction = .0129). Men with class 2 and 3 obesity (BMI 35.0 kg/m2) had a statistically significant reduction in DFS (hazard ratio [HR], 1.16; 95% confidence interval [CI], 1.01-1.33; P = .0297) compared with normal-weight patients. Underweight patients had a significantly shorter TTR and reduced DFS (HR, 1.18; 95% CI, 1.09-1.28; P < .0001) that was more significant among men (HR, 1.31; 95% CI, 1.15-1.50; P < .0001) than among women (HR, 1.11; 95% CI, 1.01-1.23; P = .0362; Pinteraction = .0340). BMI was not predictive of a benefit from adjuvant treatment. CONCLUSIONS: Obesity and underweight status were associated independently with inferior outcomes in patients with colon cancer who received treatment in adjuvant chemotherapy trials. Cancer 2013. (c) 2013 American Cancer Society. C1 [Sinicrope, Frank A.] Mayo Clin, Div Oncol, Rochester, MN 55905 USA. [Sinicrope, Frank A.; Foster, Nathan R.; Sargent, Daniel J.] North Cent Canc Treatment Grp, Rochester, MN USA. [Foster, Nathan R.; Sargent, Daniel J.] Mayo Clin, Div Biomed Stat & Informat, Rochester, MN 55905 USA. [Yothers, Greg; O'Connell, Michael J.] Natl Surg Adjuvant Breast & Bowel Project, Pittsburgh, PA USA. [Benson, Al] Eastern Cooperat Oncol Grp, Philadelphia, PA USA. [Seitz, Jean Francois] Univ Mediterranean, French Soc Digest Oncol, Marseilles, France. [Labianca, Roberto] Riuniti Hosp, Med Oncol Unit, Bergamo, Italy. [Goldberg, Richard M.] Canc & Leukemia Grp B, Chicago, IL USA. [DeGramont, Aimery] Multidisciplinary Cooperat Oncol Grp, Paris, France. RP Sinicrope, FA (reprint author), Mayo Clin, 200 1st St SW, Rochester, MN 55905 USA. EM sinicrope.frank@mayo.edu RI Goldberg , Richard/M-1311-2013; Labianca, Roberto/N-1847-2016; OI Yothers, Greg/0000-0002-7965-7333; Labianca, Roberto/0000-0001-7149-822X; Sargent, Daniel/0000-0002-2684-4741 FU National Cancer Institute Senior Scientist Award [K05CA-142885]; North Central Cooperative Treatment Group Biospecimen Resource National Institutes of Health grant [CA-114740] FX This work was supported by a National Cancer Institute Senior Scientist Award (K05CA-142885 to Dr. Sinicrope) and by a North Central Cooperative Treatment Group Biospecimen Resource National Institutes of Health grant (CA-114740). NR 40 TC 44 Z9 44 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0008-543X J9 CANCER-AM CANCER SOC JI Cancer PD APR 15 PY 2013 VL 119 IS 8 BP 1528 EP 1536 DI 10.1002/cncr.27938 PG 9 WC Oncology SC Oncology GA 126LT UT WOS:000317618700012 PM 23310947 ER PT J AU Prabhakar, U Maeda, H Jain, RK Sevick-Muraca, EM Zamboni, W Farokhzad, OC Barry, ST Gabizon, A Grodzinski, P Blakey, DC AF Prabhakar, Uma Maeda, Hiroshi Jain, Rakesh K. Sevick-Muraca, Eva M. Zamboni, William Farokhzad, Omid C. Barry, Simon T. Gabizon, Alberto Grodzinski, Piotr Blakey, David C. TI Challenges and Key Considerations of the Enhanced Permeability and Retention Effect for Nanomedicine Drug Delivery in Oncology SO CANCER RESEARCH LA English DT Editorial Material ID SOLID TUMORS; MACROMOLECULAR THERAPEUTICS; CANCER; TRANSLATION; AGENTS AB Enhanced permeability of the tumor vasculature allows macromolecules to enter the tumor interstitial space, whereas the suppressed lymphatic filtration allows them to stay there. This phenomenon, enhanced permeability and retention (EPR), has been the basis of nanotechnology platforms to deliver drugs to tumors. However, progress in developing effective drugs using this approach has been hampered by heterogeneity of EPR effect in different tumors and limited experimental data from patients on effectiveness of this mechanism as related to enhanced drug accumulation. This report summarizes the workshop discussions on key issues of the EPR effect and major gaps that need to be addressed to effectively advance nanoparticle-based drug delivery. Cancer Res; 73(8); 2412-7. (C) 2013 AACR. C1 [Prabhakar, Uma; Grodzinski, Piotr] NCI, Alliance Nanotechnol Canc, Bethesda, MD 20892 USA. [Maeda, Hiroshi] Sojo Univ, Inst DDS Res, Kumamoto, Japan. [Jain, Rakesh K.] Harvard Univ, Sch Med, Cambridge, MA 02138 USA. [Jain, Rakesh K.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Farokhzad, Omid C.] Harvard Univ, Sch Med, Boston, MA USA. [Farokhzad, Omid C.] Brigham & Womens Hosp, Boston, MA 02115 USA. [Sevick-Muraca, Eva M.] Univ Texas Hlth Sci Ctr, Brown Fdn Inst Mol Med, Houston, TX USA. [Zamboni, William] Univ N Carolina, UNC Lineberger Comprehens Canc Ctr, UNC Eshelman Sch Pharm, Chapel Hill, NC USA. [Barry, Simon T.; Blakey, David C.] AstraZeneca, Macclesfield, Cheshire, England. [Gabizon, Alberto] Shaare Zedek Med Ctr, Jerusalem, Israel. [Gabizon, Alberto] Hebrew Univ Jerusalem, Sch Med, IL-91010 Jerusalem, Israel. RP Prabhakar, U (reprint author), NCI, Off Canc Nanotechnol Res, Bldg 31 Room 10A52, Bethesda, MD 20892 USA. EM uma.prabhakar@nih.gov; grodzinp@mail.nih.gov RI Maeda, Hiroshi/N-4471-2016; Sevick-Muraca, Eva/A-4152-2017 OI Sevick-Muraca, Eva/0000-0002-8152-4847 FU NCI NIH HHS [U54 CA151652, P30 CA016086] NR 16 TC 362 Z9 364 U1 26 U2 185 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 BP 2412 EP 2417 DI 10.1158/0008-5472.CAN-12-4561 PG 6 WC Oncology SC Oncology GA 126ER UT WOS:000317595800005 PM 23423979 ER PT J AU Goldberger, N Walker, RC Kim, CH Winter, S Hunter, KW AF Goldberger, Natalie Walker, Renard C. Kim, Chang Hee Winter, Scott Hunter, Kent W. TI Inherited Variation in miR-290 Expression Suppresses Breast Cancer Progression by Targeting the Metastasis Susceptibility Gene Arid4b SO CANCER RESEARCH LA English DT Article ID EMBRYONIC STEM-CELLS; ADIPOCYTE DIFFERENTIATION; ADIPOSE-TISSUE; MICRORNAS; ESTROGEN; DISEASE; IDENTIFICATION; TAMOXIFEN; PROGNOSIS; THERAPY AB The metastatic cascade is a complex and extremely inefficient process with many potential barriers. Understanding this process is of critical importance because the majority of cancer mortality is associated with metastatic disease. Recently, it has become increasingly clear that microRNAs (miRNA) play important roles in tumorigenesis and metastasis, yet few studies have examined how germline variations may dysregulate miRNAs, in turn affecting metastatic potential. To explore this possibility, the highly metastatic MMTV-PyMT mice were crossed with 25 AKXD (AKR/J x DBA/2J) recombinant inbred strains to produce F1 progeny with varying metastatic indices. When mammary tumors from the F1 progeny were analyzed by miRNA microarray, miR-290 (containing miR-290-3p and miR-290-5p) was identified as a top candidate progression-associated miRNA. The microarray results were validated in vivo when miR-290 upregulation in two independent breast cancer cell lines suppressed both primary tumor and metastatic growth. Computational analysis identified breast cancer progression gene Arid4b as a top target of miR-290-3p, which was confirmed by luciferase reporter assay. Surprisingly, pathway analysis identified estrogen receptor (ER) signaling as the top canonical pathway affected by miR-290 upregulation. Further analysis showed that ER levels were elevated in miR-290-expressing tumors and positively correlated with apoptosis. Taken together, our results suggest miR-290 targets Arid4b while simultaneously enhancing ER signaling and increasing apoptosis, thereby suppressing breast cancer progression. This, to the best of our knowledge, is the first example of inherited differences in miRNA expression playing a role in breast cancer progression. Cancer Res; 73(8); 2671-81. (C) 2013 AACR. C1 [Goldberger, Natalie; Walker, Renard C.; Winter, Scott; Hunter, Kent W.] NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Kim, Chang Hee] NCI, Lab Mol Technol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Hunter, KW (reprint author), NCI, Metastasis Susceptibil Sect, Lab Canc Biol & Genet, CCR,NIH, Bldg 37 Room 5046D,37 Convent Dr, Bethesda, MD 20892 USA. EM hunterk@mail.nih.gov FU NCI, Center for Cancer Research, Intramural Research Program, NIH; Howard Hughes Medical Institute-NIH Research Scholars Program FX This work was supported by the NCI, Center for Cancer Research, Intramural Research Program, NIH, and the Howard Hughes Medical Institute-NIH Research Scholars Program. NR 50 TC 14 Z9 16 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2013 VL 73 IS 8 BP 2671 EP 2681 DI 10.1158/0008-5472.CAN-12-3513 PG 11 WC Oncology SC Oncology GA 126ER UT WOS:000317595800029 PM 23447578 ER PT J AU Maldonado-Baez, L Cole, NB Kramer, H Donaldson, JG AF Maldonado-Baez, Lymarie Cole, Nelson B. Kraemer, Helmut Donaldson, Julie G. TI Microtubule-dependent endosomal sorting of clathrin-independent cargo by Hook1 SO JOURNAL OF CELL BIOLOGY LA English DT Article ID ENDOCYTIC PATHWAY; RECYCLING PATHWAY; PLASMA-MEMBRANE; UNIQUE PLATFORM; PROTEINS; ARF6; TRAFFICKING; MECHANISMS; CELL; REQUIREMENT AB Many plasma membrane (PM) proteins enter cells nonselectively through clathrin-independent endocytosis (CIE). Here, we present evidence that cytoplasmic sequences in three CIE cargo proteins-CD44, CD98, and CD147-were responsible for the rapid sorting of these proteins into endosomal tubules away from endosomes associated with early endosomal antigen 1 (EEA1). We found that Hook1, a microtubule-and cargo-tethering protein, recognized the cytoplasmic tail of CD147 to help sort it and CD98 into Rab22a-dependent tubules associated with recycling. Depletion of Hook1 from cells altered trafficking of CD44, CD98, and CD147 toward EEA1 compartments and impaired the recycling of CD98 back to the PM. In contrast, another CIE cargo protein, major histocompatibility complex class I, which normally traffics to EEA1 compartments, was not affected by depletion of Hook1. Loss of Hook1 also led to an inhibition of cell spreading, implicating a role for Hook1 sorting of specific CIE cargo proteins away from bulk membrane and back to the PM. C1 [Maldonado-Baez, Lymarie; Cole, Nelson B.; Donaldson, Julie G.] NHLBI, Cell Biol Lab, Cell Biol & Physiol Ctr, NIH, Bethesda, MD 20892 USA. [Kraemer, Helmut] Univ Texas SW Med Ctr Dallas, Dept Cell Biol, Dallas, TX 75390 USA. [Kraemer, Helmut] Univ Texas SW Med Ctr Dallas, Dept Neurosci, Dallas, TX 75390 USA. RP Donaldson, JG (reprint author), NHLBI, Cell Biol Lab, Cell Biol & Physiol Ctr, NIH, Bethesda, MD 20892 USA. EM donaldsonj@helix.nih.gov OI Kramer, Helmut/0000-0002-1167-2676 FU Intramural Research Program in the National Heart, Lung, and Blood Institute at the National Institutes of Health [HL006060]; National Eye Institute [EY10199] FX This work was supported by the Intramural Research Program in the National Heart, Lung, and Blood Institute at the National Institutes of Health (HL006060) and a grant from the National Eye Institute (EY10199) to H. Kramer. NR 52 TC 41 Z9 41 U1 1 U2 12 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD APR 15 PY 2013 VL 201 IS 2 BP 233 EP 247 DI 10.1083/jcb.201208172 PG 15 WC Cell Biology SC Cell Biology GA 126AM UT WOS:000317583500008 PM 23589492 ER PT J AU Balagopalan, L Barr, VA Kortum, RL Park, AK Samelson, LE AF Balagopalan, Lakshmi Barr, Valarie A. Kortum, Robert L. Park, Anna K. Samelson, Lawrence E. TI Cutting Edge: Cell Surface Linker for Activation of T Cells Is Recruited to Microclusters and Is Active in Signaling SO JOURNAL OF IMMUNOLOGY LA English DT Article ID IMMUNOLOGICAL SYNAPSE; ANTIGEN RECEPTOR; LAT; PHOSPHORYLATION; COMPLEXES; VESICLES; DISTINCT AB A controversy has recently emerged regarding the location of the cellular pool of the adapter linker for activation of T cells (LAT) that participates in propagation of signals downstream of the TCR. In one model phosphorylation and direct recruitment of cell surface LAT to activation-induced microclusters is critical for T cell activation, whereas in the other model vesicular, but not surface, LAT participates in these processes. By using a chimeric version of LAT that can be tracked via an extracellular domain, we provide evidence that LAT located at the cell surface can be recruited efficiently to activation-induced microclusters within seconds of TCR engagement. Importantly, we also demonstrate that this pool of LAT at the plasma membrane is rapidly phosphorylated. Our results provide support for the model in which the cell utilizes LAT from the cell surface for rapid responses to TCR stimulation. The Journal of Immunology, 2013, 190: 3849-3853. C1 [Balagopalan, Lakshmi; Barr, Valarie A.; Kortum, Robert L.; Park, Anna K.; Samelson, Lawrence E.] NCI, Lab Cellular & Mol Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Samelson, LE (reprint author), NCI, Lab Cellular & Mol Biol, Ctr Canc Res, NIH, Bldg 37,Room 2066, Bethesda, MD 20892 USA. EM samelsonl@helix.nih.gov FU Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research FX This work was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. NR 15 TC 22 Z9 22 U1 0 U2 3 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 2013 VL 190 IS 8 BP 3849 EP 3853 DI 10.4049/jimmunol.1202760 PG 5 WC Immunology SC Immunology GA 121WC UT WOS:000317274500003 PM 23487428 ER PT J AU Rosen, LB Freeman, AF Yang, LM Jutivorakool, K Olivier, KN Angkasekwinai, N Suputtamongkol, Y Bennett, JE Pyrgos, V Williamson, PR Ding, L Holland, SM Browne, SK AF Rosen, Lindsey B. Freeman, Alexandra F. Yang, Lauren M. Jutivorakool, Kamonwan Olivier, Kenneth N. Angkasekwinai, Nasikarn Suputtamongkol, Yupin Bennett, John E. Pyrgos, Vasilios Williamson, Peter R. Ding, Li Holland, Steven M. Browne, Sarah K. TI Anti-GM-CSF Autoantibodies in Patients with Cryptococcal Meningitis SO JOURNAL OF IMMUNOLOGY LA English DT Article ID PULMONARY ALVEOLAR PROTEINOSIS; COLONY-STIMULATING FACTOR; CHRONIC MUCOCUTANEOUS CANDIDIASIS; IFN-GAMMA AUTOANTIBODY; NECROSIS-FACTOR-ALPHA; RITUXIMAB THERAPY; ANTICRYPTOCOCCAL ACTIVITY; MYCOBACTERIAL INFECTION; NOCARDIA-ASTEROIDES; PEMPHIGUS-VULGARIS AB Cryptococcal meningitis has been described in immunocompromised patients, as well as in those for whom no immune defect has been identified. GM-CSF regulates the function of phagocytes and pulmonary alveolar macrophages, critical elements in cryptococcal control. We performed clinical histories, immunological evaluation, and anticytokine autoantibody screening in four current patients with cryptococcal meningitis and identified and tested 103 archived plasma/cerebrospinal fluid samples from patients with cryptococcal meningitis. We assessed the ability of anti-GM-CSF autoantibody-containing plasmas to inhibit GM-CSF signaling. We recognized anti-GM-CSF autoantibodies in an otherwise healthy female with cryptococcal meningitis who later developed pulmonary alveolar proteinosis (PAP). Her diagnosis prompted screening of patients with cryptococcal meningitis for anticytokine autoantibodies. We identified seven HIV-negative patients with cryptococcal meningitis who tested positive for high-titer anti-GM-CSF autoantibodies. Two of the seven later developed evidence of PAP. Plasma from all patients prevented GM-CSF-induced STAT5 phosphorylation and MIP-1 alpha production in normal PBMCs. This effect was limited to their IgG fraction. Anti-GM-CSF autoantibodies are associated with some cases of cryptococcal meningitis in otherwise immunocompetent patients. These cases need not have associated PAP. The Journal of Immunology, 2013, 190: 3959-3966. C1 [Rosen, Lindsey B.; Freeman, Alexandra F.; Yang, Lauren M.; Jutivorakool, Kamonwan; Olivier, Kenneth N.; Bennett, John E.; Pyrgos, Vasilios; Williamson, Peter R.; Ding, Li; Holland, Steven M.; Browne, Sarah K.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Yang, Lauren M.] Washington Univ, Sch Med, St Louis, MO 63110 USA. [Jutivorakool, Kamonwan] Chulalongkorn Univ, Dept Med, Fac Med, Bangkok 10330, Thailand. [Jutivorakool, Kamonwan] King Chulalongkorn Mem Hosp, Thai Red Cross Soc, Bangkok 10330, Thailand. [Angkasekwinai, Nasikarn; Suputtamongkol, Yupin] Mahidol Univ, Siriraj Hosp, Fac Med, Bangkok 10700, Thailand. RP Browne, SK (reprint author), NIAID, NIH, 9000 Rock Ville Pike,MSC 1684,10 Clin Res Ctr Rm, Bethesda, MD 20892 USA. EM brownesa@niaid.nih.gov FU Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health FX This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health. NR 52 TC 48 Z9 48 U1 1 U2 6 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 2013 VL 190 IS 8 BP 3959 EP 3966 DI 10.4049/jimmunol.1202526 PG 8 WC Immunology SC Immunology GA 121WC UT WOS:000317274500015 PM 23509356 ER PT J AU Park, H Adamson, L Ha, T Mullen, K Hagen, SI Nogueron, A Sylwester, AW Axthelm, MK Legasse, A Piatak, M Lifson, JD McElrath, JM Picker, LJ Seder, RA AF Park, Haesun Adamson, Lauren Ha, Tae Mullen, Karl Hagen, Shoko I. Nogueron, Arys Sylwester, Andrew W. Axthelm, Michael K. Legasse, Al Piatak, Michael, Jr. Lifson, Jeffrey D. McElrath, Juliana M. Picker, Louis J. Seder, Robert A. TI Polyinosinic-Polycytidylic Acid Is the Most Effective TLR Adjuvant for SIV Gag Protein-Induced T Cell Responses In Nonhuman Primates SO JOURNAL OF IMMUNOLOGY LA English DT Article ID SIMIAN IMMUNODEFICIENCY VIRUS; DENDRITIC CELLS; INNATE IMMUNITY; RHESUS-MONKEYS; VACCINE DEVELOPMENT; CROSS-PRESENTATION; I INTERFERON; IMMUNIZATION; REPLICATION; PROTECTION AB Prime-boost immunization with heterologous vaccines elicits potent cellular immunity. In this study, we assessed the influence of various TLR ligands on SIV Gag-specific T cell immunity and protection following prime-boost immunization. Rhesus macaques (RMs) were primed with SIV Gag protein emulsified in Montanide ISA51 with or without TLR3 (polyinosinic-polycytidylic acid [poly-IC]), TLR4 (monophosphoryl lipid A), TLR7/8 (3M-012), TLR9 (CpG), or TLR3 (poly-IC) combined with TLR7/8 ligands, then boosted with replication defective adenovirus 5 expressing SIV Gag (rAd5-Gag). After priming, RMs that received SIV Gag protein plus poly-IC developed significantly higher frequencies of SIV Gag-specific CD4(+) Th1 responses in blood and bronchoalveolar lavage (BAL) fluid lymphocytes compared with all other adjuvants, and low-level SIV Gag-specific CD8(+) T cell responses. After the rAd5-Gag boost, the magnitude and breadth of SIV Gag-specific CD8(+) T cell responses were significantly increased in RM primed with SIV Gag protein plus poly-IC, with or without the TLR7/8 ligand, or CpG. However, the anamnestic, SIV Gag-specific CD8(+) T cell response to SIVmac251 challenge was not significantly enhanced by SIV Gag protein priming with any of the adjuvants. In contrast, the anamnestic SIV Gag-specific CD4(+) T cell response in BAL was enhanced by SIV Gag protein priming with poly-IC or CpG, which correlated with partial control of early viral replication after SIVmac251 challenge. These results demonstrate that prime-boost vaccination with SIV Gag protein/poly-IC improves magnitude, breadth, and durability of CD4(+) T cell immune responses, which could have a role in the control of SIV viral replication. The Journal of Immunology, 2013, 190: 4103-4115. C1 [Park, Haesun; Adamson, Lauren; Ha, Tae; Mullen, Karl; Hagen, Shoko I.; Nogueron, Arys; Sylwester, Andrew W.; Axthelm, Michael K.; Legasse, Al; Picker, Louis J.] Oregon Hlth & Sci Univ, Dept Pathol, Vaccine & Gene Therapy Inst, Beaverton, OR 97006 USA. [Park, Haesun; Adamson, Lauren; Ha, Tae; Mullen, Karl; Hagen, Shoko I.; Nogueron, Arys; Sylwester, Andrew W.; Axthelm, Michael K.; Legasse, Al; Picker, Louis J.] Oregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Beaverton, OR 97006 USA. [Piatak, Michael, Jr.] NCI, AIDS Vaccine Program, Sci Applicat Int Corp Frederick, Frederick, MD 21702 USA. [Lifson, Jeffrey D.] Frederick Natl Lab, AIDS & Canc Dev Program, Frederick, MD 21702 USA. [McElrath, Juliana M.] Fred Hutchinson Canc Res Ctr, Clin Res Div, Seattle, WA 98109 USA. [Seder, Robert A.] NIH, Cellular Immunol Sect, Vaccine Res Ctr, Bethesda, MD 20892 USA. RP Seder, RA (reprint author), NIH, Cellular Immunol Sect, Vaccine Res Ctr, 40 Convent Dr,MSC 3025,Bldg 40,Room 3512, Bethesda, MD 20892 USA. EM rseder@mail.nih.gov FU Bill and Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery [38645]; federal funds from the National Cancer Institute, National Institutes of Health [HHSN261200800001E] FX This work was supported by the Bill and Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery (Grant 38645). This work was funded in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract HHSN261200800001E. NR 53 TC 23 Z9 25 U1 1 U2 15 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 2013 VL 190 IS 8 BP 4103 EP 4115 DI 10.4049/jimmunol.1202958 PG 13 WC Immunology SC Immunology GA 121WC UT WOS:000317274500029 PM 23509365 ER PT J AU Jones-Davis, DM Yang, M Rider, E Osbun, NC da Gente, GJ Li, J Katz, AM Weber, MD Sen, S Crawley, J Sherr, EH AF Jones-Davis, Dorothy M. Yang, Mu Rider, Eric Osbun, Nathan C. da Gente, Gilberto J. Li, Jiang Katz, Adam M. Weber, Michael D. Sen, Saunak Crawley, Jacqueline Sherr, Elliott H. TI Quantitative Trait Loci for Interhemispheric Commissure Development and Social Behaviors in the BTBR T+ tf/J Mouse Model of Autism SO PLOS ONE LA English DT Article ID CORPUS-CALLOSUM; SPECTRUM DISORDERS; WHITE-MATTER; T+TF/J MICE; STRAINS; SOCIABILITY; RECURRENCE; PHENOTYPES; AGENESIS; DEFICITS AB Background: Autism and Agenesis of the Corpus Callosum (AgCC) are interrelated behavioral and anatomic phenotypes whose genetic etiologies are incompletely understood. We used the BTBR T+ tf/ J (BTBR) strain, exhibiting fully penetrant AgCC, a diminished hippocampal commissure, and abnormal behaviors that may have face validity to autism, to study the genetic basis of these disorders. Methods: We generated 410 progeny from an F2 intercross between the BTBR and C57BL/ 6J strains. The progeny were phenotyped for social behaviors (as juveniles and adults) and commisural morphology, and genotyped using 458 markers. Quantitative trait loci (QTL) were identified using genome scans; significant loci were fine-mapped, and the BTBR genome was sequenced and analyzed to identify candidate genes. Results: Six QTL meeting genome-wide significance for three autism-relevant behaviors in BTBR were identified on chromosomes 1, 3, 9, 10, 12, and X. Four novel QTL for commissural morphology on chromosomes 4, 6, and 12 were also identified. We identified a highly significant QTL (LOD score = 20.2) for callosal morphology on the distal end of chromosome 4. Conclusions: We identified several QTL and candidate genes for both autism-relevant traits and commissural morphology in the BTBR mouse. Twenty-nine candidate genes were associated with synaptic activity, axon guidance, and neural development. This is consistent with a role for these processes in modulating white matter tract development and aspects of autism-relevant behaviors in the BTBR mouse. Our findings reveal candidate genes in a mouse model that will inform future human and preclinical studies of autism and AgCC. C1 [Jones-Davis, Dorothy M.; Rider, Eric; Osbun, Nathan C.; da Gente, Gilberto J.; Li, Jiang; Sherr, Elliott H.] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA. [Yang, Mu; Katz, Adam M.; Weber, Michael D.; Crawley, Jacqueline] NIMH, Lab Behav Neurosci, Intramural Res Program, NIH, Bethesda, MD 20892 USA. [Sen, Saunak] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. RP Sherr, EH (reprint author), Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA. EM sherre@neuropeds.ucsf.edu OI Jones-Davis, Dorothy/0000-0001-7209-9183 FU Pfizer, Inc. [P0030953]; MH02179 from the National Institute of Mental Health Intramural Research Program [MH02179]; National Institutes of Health [K02NS052192, NS062173]; National Institute of General Medical Sciences/National Institutes of Health [K12GM081266] FX This work was supported by Grant P0030953 from Pfizer, Inc.(http://www.pfizer.com) to Dr. Elliott Sherr. Dr. Mathew Pletcher (employed by Pfizer, Inc.) provided suggestions in the design and interpretation of the study, and Dr. Nicholas Brandon (employed by Pfizer, Inc.) provided a critical reading of the manuscript. This work was also supported by Award MH02179 from the National Institute of Mental Health Intramural Research Program to Dr. Jacqueline Crawley and Dr. Mu Yang, National Institutes of Health Awards K02NS052192 and NS062173 to Dr.Elliott Sherr, and Award Number K12GM081266 from the National Institute of General Medical Sciences/National Institutes of Health (http://projectreporter.nih.gov/project_ info_ description.cfm? aid = 7869455& icde = 12962536& ddparam = & ddvalue = & ddsub = & cr = 443& csb = GNA& cs = ASC) to Dr. Dorothy Jones-Davis. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 14 Z9 14 U1 0 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 15 PY 2013 VL 8 IS 4 AR e61829 DI 10.1371/journal.pone.0061829 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125TD UT WOS:000317563300039 PM 23613947 ER PT J AU Undrovinas, A Maltsev, VA Sabbah, HN AF Undrovinas, Albertas Maltsev, Victor A. Sabbah, Hani N. TI Calpain Inhibition Reduces Amplitude and Accelerates Decay of the Late Sodium Current in Ventricular Myocytes from Dogs with Chronic Heart Failure SO PLOS ONE LA English DT Article ID GUINEA-PIG HEART; INTRACELLULAR CALCIUM; ANTIANGINAL AGENT; FAILING HEART; CANINE MODEL; CA CURRENT; CELL; CHANNEL; CARDIOMYOCYTES; PROTEOLYSIS AB Calpain is an intracellular Ca2+ -activated protease that is involved in numerous Ca2+ dependent regulation of protein function in many cell types. This paper tests a hypothesis that calpains are involved in Ca2+ -dependent increase of the late sodium current (I-NaL) in failing heart. Chronic heart failure (HF) was induced in 2 dogs by multiple coronary artery embolization. Using a conventional patch-clamp technique, the whole-cell INaL was recorded in enzymatically isolated ventricular cardiomyocytes (VCMs) in which INaL was activated by the presence of a higher (1 mu M) intracellular [Ca2+] in the patch pipette. Cell suspensions were exposed to a cell-permeant calpain inhibitor MDL-28170 for 1-2 h before INaL recordings. The numerical excitation-contraction coupling (ECC) model was used to evaluate electrophysiological effects of calpain inhibition in silico. MDL caused acceleration of INaL decay evaluated by the two-exponential fit (tau(1)= 42 +/- 3.0 ms tau(2) = 435 +/- 27 ms, n = 6, in MDL vs. tau(1)= 52 +/- 2.1 ms tau(2) = 605 +/- 26 control no vehicle, n = 11, and vs. tau(1)= 52 +/- 2.8 ms tau(2) = 583 +/- 37 ms n = 7, control with vehicle, P<0.05 ANOVA). MDL significantly reduced I-NaL density recorded at -30 mV (0.488 +/- 0.03, n = 12, in control no vehicle, 0.4502 +/- 0.0210, n = 9 in vehicle vs. 0.166 +/- 0.05pA/pF, n = 5, in MDL). Our measurements of current-voltage relationships demonstrated that the INaL density was decreased by MDL in a wide range of potentials, including that for the action potential plateau. At the same time the membrane potential dependency of the steady-state activation and inactivation remained unchanged in the MDL-treated VCMs. Our ECC model predicted that calpain inhibition greatly improves myocyte function by reducing the action potential duration and intracellular diastolic Ca2+ accumulation in the pulse train. Conclusions: Calpain inhibition reverses INaL changes in failing dog ventricular cardiomyocytes in the presence of high intracellular Ca2+. Specifically it decreases INaL density and accelerates INaL kinetics resulting in improvement of myocyte electrical response and Ca2+ handling as predicted by our in silico simulations. C1 [Undrovinas, Albertas; Sabbah, Hani N.] Henry Ford Hosp, Dept Internal Med, Detroit, MI 48202 USA. [Maltsev, Victor A.] NIA, Intramural Res Program, Baltimore, MD 21224 USA. RP Undrovinas, A (reprint author), Henry Ford Hosp, Dept Internal Med, Detroit, MI 48202 USA. EM adas7247@yahoo.com FU National Heart, Lung, and Blood Institute [HL-53819, HL-074238]; American Heart Association [0350472Z]; Intramural Research Program of the National Institute on Aging FX This study was supported by National Heart, Lung, and Blood Institute Grants HL-53819 and HL-074238, by a grant-in-aid from the American Heart Association (0350472Z; to AU), and, in part, by the Intramural Research Program of the National Institute on Aging (to VM; the numerical modeling part). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 6 Z9 6 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 15 PY 2013 VL 8 IS 4 AR e54436 DI 10.1371/journal.pone.0054436 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125TD UT WOS:000317563300001 PM 23596505 ER PT J AU Kozma, E Gizewski, ET Tosh, DK Squarcialupi, L Auchampach, JA Jacobson, KA AF Kozma, Eszter Gizewski, Elizabeth T. Tosh, Dilip K. Squarcialupi, Lucia Auchampach, John A. Jacobson, Kenneth A. TI Characterization by flow cytometry of fluorescent, selective agonist probes of the A(3) adenosine receptor SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE Purines; Fluorescence; G protein-coupled receptor; A(3) adenosine receptor; Flow cytometry ID A3 RECEPTORS; LIVING CELLS; INTERNALIZATION; DESENSITIZATION; PYRENE; NUCLEOSIDES; BINDING; A(2A); RESENSITIZATION; MICRODOMAINS AB Various fluorescent nucleoside agonists of the A(3) adenosine receptor (AR) were compared as high affinity probes using radioligands and flow cytometry (FCM). They contained a fluorophore linked through the C2 or N-6 position and rigid A(3)AR-enhancing (N)-methanocarba modification. A hydrophobic C2-(1-pyrenyl) derivative MRS5704 bound nonselectively. C2-Tethered cyanine5-dye labeled MRS5218 bound selectively to hA(3)AR expressed in whole CHO cells and membranes. By FCM, binding was A(3)AR-mediated (blocked by A(3)AR antagonist, at least half through internalization), with t(1/2) for association 38 min in mA(3)AR-HEK293 cells; 26.4 mm in sucrose-treated hA(3)AR-CHO cells (K-d 31 nM). Membrane binding indicated moderate mA(3)AR affinity, but not selectivity. Specific accumulation of fluorescence (50 nM MRS5218) occurred in cells expressing mA(3)AR, but not other mouse ARs. Evidence was provided suggesting that MRS5218 detects endogenous expression of the A(3)AR in the human promyelocytic leukemic HL-60 cell line. Therefore, MRS5218 promises to be a useful tool for characterizing the A(3)AR. Published by Elsevier Inc. C1 [Kozma, Eszter; Tosh, Dilip K.; Squarcialupi, Lucia; Jacobson, Kenneth A.] NIDDKD, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. [Gizewski, Elizabeth T.; Auchampach, John A.] Med Coll Wisconsin, Dept Pharmacol & Toxicol, Milwaukee, WI 53226 USA. [Gizewski, Elizabeth T.; Auchampach, John A.] Med Coll Wisconsin, Ctr Cardiovasc, Milwaukee, WI 53226 USA. RP Jacobson, KA (reprint author), NIDDKD, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bldg 8A,Rm B1A-19, Bethesda, MD 20892 USA. EM kajacobs@helix.nih.gov RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU National Institutes of Health [R01HL077707]; Intramural Research Program of National Institute of Diabetes and Digestive and Kidney Diseases; Hungarian-American Enterprise Scholarship Foundation (HAESF); University of Florence, Italy FX This research was supported by the National Institutes of Health (R01HL077707) and the Intramural Research Program of National Institute of Diabetes and Digestive and Kidney Diseases. EK thanks the Hungarian-American Enterprise Scholarship Foundation (HAESF) for financial support. LS thanks the University of Florence, Italy for financial support. NR 39 TC 7 Z9 7 U1 0 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD APR 15 PY 2013 VL 85 IS 8 BP 1171 EP 1181 DI 10.1016/j.bcp.2013.01.021 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 115XS UT WOS:000316845800015 PM 23376019 ER PT J AU Theberge, FR Li, X Kambhampati, S Pickens, CL Laurent, RS Bossert, JM Baumann, MH Hutchinson, MR Rice, KC Watkins, LR Shaham, Y AF Theberge, Florence R. Li, Xuan Kambhampati, Santa Pickens, Charles L. Laurent, Robyn St. Bossert, Jennifer M. Baumann, Michael H. Hutchinson, Mark R. Rice, Kenner C. Watkins, Linda R. Shaham, Yavin TI Effect of Chronic. Delivery of the Toll-like Receptor 4 Antagonist (+)-Naltrexone on Incubation of Heroin Craving SO BIOLOGICAL PSYCHIATRY LA English DT Article DE Craving; extinction; glia; heroin self-administration; opioid drugs; reinstatement; relapse; TLR4 ID FACTOR-KAPPA-B; STRESS-INDUCED REINSTATEMENT; DOPAMINE D-1-FAMILY RECEPTORS; VENTRAL TEGMENTAL AREA; TIME-DEPENDENT CHANGES; CENTRAL-NERVOUS-SYSTEM; NUCLEUS-ACCUMBENS; COCAINE-SEEKING; DRUG-DEPENDENCE; CONDITIONED IMMUNOMODULATION AB Background: Recent evidence implicates toll-like receptor 4 (TLR4) in opioid analgesia, tolerance, conditioned place preference, and self-administration. Here, we determined the effect of the TLR4 antagonist (+)-naltrexone (a mu-opioid receptor inactive isomer) on the time-dependent increases in cue-induced heroin seeking after withdrawal (incubation of heroin craving). Methods: In an initial experiment, we trained rats for 9 hours per day to self-administer heroin (.1 mg/kg/infusion) for 9 days; lever presses were paired with a 5-second tone-light cue. We then assessed cue-induced heroin seeking in 30-minute extinction sessions on withdrawal day 1; immediately after testing, we surgically implanted rats with Alzet minipumps delivering (+)-naltrexone (0, 7.5, 15, 30 mg/kg/day, subcutaneous) for 14 days. We then tested the rats for incubated cue-induced heroin seeking in 3-hour extinction tests on withdrawal day 13. Results: We found that chronic delivery of (+)-naltrexone via minipumps during the withdrawal phase decreased incubated cue-induced heroin seeking. In follow-up experiments, we found that acute injections of (+)-naltrexone immediately before withdrawal day 13 extinction tests had no effect on incubated cue-induced heroin seeking. Furthermore, chronic delivery of (+)-naltrexone (15 or 30 mg/kg/day) or acute systemic injections (15 or 30 mg/kg) had no effect on ongoing extended access heroin self-administration. Finally, in rats trained to self-administer methamphetamine (.1 mg/kg/infusion, 9 hours/day, 9 days), chronic delivery of (+)-naltrexone (30 mg/kg/day) during the withdrawal phase had no effect on incubated cue-induced methamphetamine seeking. Conclusions: The present results suggest a critical role of TLR4 in the development of incubation of heroin, but not methamphetamine, craving. C1 [Theberge, Florence R.; Li, Xuan; Kambhampati, Santa; Pickens, Charles L.; Laurent, Robyn St.; Bossert, Jennifer M.; Baumann, Michael H.; Rice, Kenner C.; Shaham, Yavin] Natl Inst Drug Abuse, Intramural Res Program, NIH, Baltimore, MD USA. [Hutchinson, Mark R.] Univ Adelaide, Discipline Physiol, Sch Med Sci, Adelaide, SA, Australia. [Watkins, Linda R.] Univ Colorado, Dept Psychol, Boulder, CO 80309 USA. [Watkins, Linda R.] Univ Colorado, Dept Neurosci, Boulder, CO USA. RP Shaham, Y (reprint author), NIDA, IRP, NIH, Behav Neurosci Branch, 251 Bayview Blvd, Baltimore, MD 21224 USA. EM yshaham@intra.nida.nih.gov RI shaham, yavin/G-1306-2014; Hutchinson, Mark/G-4147-2014 OI Hutchinson, Mark/0000-0003-2154-5950 FU National Institute on Drug Abuse; National Institute on Alcohol Abuse and Alcoholism; Australian Research Council [DP110100297]; National Institute on Drug Abuse [K05-DA024044] FX The work was supported by the Intramural Research Programs of the National Institute on Drug Abuse and National Institute on Alcohol Abuse and Alcoholism. MRH is supported by an Australian Research Council Research Fellowship (DP110100297). LRW is supported by National Institute on Drug Abuse K05-DA024044. KCR is also affiliated with the Intramural Research Program of National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health. NR 79 TC 30 Z9 31 U1 2 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2013 VL 73 IS 8 BP 729 EP 737 DI 10.1016/j.biopsych.2012.12.019 PG 9 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 120GT UT WOS:000317159500007 PM 23384483 ER PT J AU Schank, JR Tapocik, JD Barbier, E Damadzic, R Eskay, RL Sun, H Rowe, KE King, CE Yao, MD Flanigan, ME Solomon, MG Karlsson, C Cheng, KJ Rice, KC Heilig, M AF Schank, Jesse R. Tapocik, Jenica D. Barbier, Estelle Damadzic, Ruslan Eskay, Robert L. Sun, Hui Rowe, Kelly E. King, Courtney E. Yao, Mengdi Flanigan, Meghan E. Solomon, Matthew G. Karlsson, Camilla Cheng, Kejun Rice, Kenner C. Heilig, Markus TI Tacr1 Gene Variation and Neurokinin 1 Receptor Expression Is Associated with Antagonist Efficacy in Genetically Selected Alcohol-Preferring Rats SO BIOLOGICAL PSYCHIATRY LA English DT Article DE Alcohol; amygdala; neurokinin; P-rat; self-administration; Substance P ID NONPREFERRING NP RATS; SUBSTANCE-P; PRIMING INJECTIONS; ANIMAL-MODELS; MICE LACKING; STRESS; ANXIETY; ETHANOL; CONSUMPTION; EXPOSURE AB Background: Genetic deletion or antagonism of the neurokinin 1 receptor (NK1R) decreases alcohol intake, alcohol reward, and stress-induced alcohol relapse in rodents, while TACR1 variation is associated with alcoholism in humans. Methods: We used L822429, a specific antagonist with high affinity for the rat NK1R, and examined whether sensitivity to NK1R blockade is altered in alcohol-preferring (P) rats. Operant alcohol self-administration and progressive ratio responding were analyzed in P-rats and their founder Wistar line. We also analyzed Tacr1 expression and binding and sequenced the Tacr7 promoter from both lines. Results: Systemic L822429 decreased alcohol self-administration in P-rats but did not affect the lower rates of alcohol self-administration in Wistar rats. Tacr1 expression was elevated in the prefrontal cortex and the amygdala of P-rats. In central amygdala, elevated Tacr1 expression was accompanied by elevated NK1R binding. Central amygdala (but not prefrontal cortex) infusion of L822429 replicated the systemic antagonist effects on alcohol self-administration in P-rats. All P-rats, but only 18% of their founder Wistar population, were CC homozygous for a -1372G/C single nucleotide polymorphism. In silico analysis indicated that the Tacr1 -1372 genotype could modulate binding of the transcription factors GATA-2 and E2F-1. Electromobility shift and luciferase reporter assays suggested that the -1372C allele confers increased transcription factor binding and transcription. Conclusions: Genetic variation at the Tacr1 locus may contribute to elevated rates of alcohol self-administration, while at the same time increasing sensitivity to NK1R antagonist treatment. C1 [Schank, Jesse R.; Tapocik, Jenica D.; Barbier, Estelle; Damadzic, Ruslan; Eskay, Robert L.; Sun, Hui; Rowe, Kelly E.; King, Courtney E.; Yao, Mengdi; Flanigan, Meghan E.; Solomon, Matthew G.; Karlsson, Camilla; Heilig, Markus] NIAAA, Lab Clin & Translat Studies, Bethesda, MD 20892 USA. [Cheng, Kejun; Rice, Kenner C.] Natl Inst Drug Abuse, Chem Biol Branch, Bethesda, MD USA. NIAAA, NIH, Bethesda, MD 20892 USA. RP Heilig, M (reprint author), NIAAA, Lab Clin & Translat Studies, NIH, 10 Ctr Dr,Room 10 CRC 1E-5334, Bethesda, MD 20892 USA. EM markus.heilig@mail.nih.gov OI Heilig, Markus/0000-0003-2706-2482; Flanigan, Meghan/0000-0002-3185-7459 FU National Institute on Alcohol Abuse and Alcoholism [R24 AA015512] FX We thank the Indiana University for providing the alcohol-preferring rats for these experiments. The generation of these animals is supported by the R24 Alcohol Research Resource Award Grant (R24 AA015512) from the National Institute on Alcohol Abuse and Alcoholism. We thank Dr. Yavin Shaham for valuable discussion and comments on the manuscript and Dr. Jennifer Bossert for training on procedures. NR 33 TC 12 Z9 12 U1 1 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2013 VL 73 IS 8 BP 774 EP 781 DI 10.1016/j.biopsych.2012.12.027 PG 8 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 120GT UT WOS:000317159500012 PM 23419547 ER PT J AU Ali, M Sur, D You, YA Kanungo, S Sah, B Manna, B Puri, M Wierzba, TF Donner, A Nair, GB Bhattacharya, SK Dhingra, MS Deen, JL Lopez, AL Clemens, J AF Ali, Mohammad Sur, Dipika You, Young Ae Kanungo, Suman Sah, Binod Manna, Byomkesh Puri, Mahesh Wierzba, Thomas F. Donner, Allan Nair, G. Balakrish Bhattacharya, Sujit K. Dhingra, Mandeep Singh Deen, Jacqueline L. Lopez, Anna Lena Clemens, John TI Herd Protection by a Bivalent Killed Whole-Cell Oral Cholera Vaccine in the Slums of Kolkata, India SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE cholera; herd effect; bivalent killed oral cholera vaccine; cluster randomized trial ID CONJUGATE VACCINE; FIELD TRIAL; IMMUNITY; IMMUNIZATION; BANGLADESH; PERTUSSIS; EFFICACY; CHILDREN; MEASLES; DISEASE AB Background. We evaluated the herd protection conferred by an oral cholera vaccine using 2 approaches: cluster design and geographic information system (GIS) design. Methods. Residents living in 3933 dwellings (clusters) in Kolkata, India, were cluster-randomized to receive either cholera vaccine or oral placebo. Nonpregnant residents aged >= 1 year were invited to participate in the trial. Only the first episode of cholera detected for a subject between 14 and 1095 days after a second dose was considered. In the cluster design, indirect protection was assessed by comparing the incidence of cholera among nonparticipants in vaccine clusters vs those in placebo clusters. In the GIS analysis, herd protection was assessed by evaluating association between vaccine coverage among the population residing within 250 m of the household and the occurrence of cholera in that population. Results. Among 107 347 eligible residents, 66 990 received 2 doses of either cholera vaccine or placebo. In the cluster design, the 3-year data showed significant total protection (66% protection, 95% confidence interval [CI], 50%-78%, P < .01) but no evidence of indirect protection. With the GIS approach, the risk of cholera among placebo recipients was inversely related to neighborhood-level vaccine coverage, and the trend was highly significant (P < .01). This relationship held in multivariable models that also controlled for potentially confounding demographic variables (hazard ratio, 0.94 [95%CI, .90-.98]; P < .01). Conclusions. Indirect protection was evident in analyses using the GIS approach but not the cluster design approach, likely owing to considerable transmission of cholera between clusters, which would vitiate herd protection in the cluster analyses. C1 [Ali, Mohammad; You, Young Ae; Sah, Binod; Puri, Mahesh; Wierzba, Thomas F.; Lopez, Anna Lena; Clemens, John] Int Vaccine Inst, Seoul 151919, South Korea. [Sur, Dipika; Kanungo, Suman; Manna, Byomkesh; Nair, G. Balakrish] Natl Inst Cholera & Enter Dis, Kolkata, India. [Donner, Allan] Univ Western Ontario, London, ON, Canada. [Bhattacharya, Sujit K.] Soc Appl Studies, Kolkata, India. [Dhingra, Mandeep Singh] Shantha Biotech, Hyderabad, Andhra Pradesh, India. [Deen, Jacqueline L.] Menzies Sch Hlth Res, Casuarina, NT, Australia. [Lopez, Anna Lena] Univ Philippines Manila, NIH, Los Angeles, CA USA. [Clemens, John] Univ Calif Los Angeles, Sch Publ Hlth, Los Angeles, CA 90024 USA. RP Ali, M (reprint author), Int Vaccine Inst, SNU Res Pk,San 4-8 Nakseongdae Dong, Seoul 151919, South Korea. EM mali@ivi.int RI Ali, Mohammad/E-2365-2017; OI Ali, Mohammad/0000-0003-1410-388X; Dhingra, Mandeep Singh/0000-0002-2507-7165 FU Bill & Melinda Gates Foundation through the Diseases of the Most Impoverished Program; Cholera Vaccine Initiative; Swedish International Development Cooperation Agency; government of South Korea; government of Sweden; government of Kuwait FX This work was supported by the Bill & Melinda Gates Foundation through the Diseases of the Most Impoverished Program and the Cholera Vaccine Initiative. Additional funding is provided by the Swedish International Development Cooperation Agency and the governments of South Korea, Sweden, and Kuwait. Shantha Biotechnics donated vaccine and placebo for the study. No funding bodies had any role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 31 TC 19 Z9 19 U1 0 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD APR 15 PY 2013 VL 56 IS 8 BP 1123 EP 1131 DI 10.1093/cid/cit009 PG 9 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 113WM UT WOS:000316700100014 PM 23362293 ER PT J AU Pace, JE Siberry, GK Hazra, R Kapogiannis, BG AF Pace, Jill E. Siberry, George K. Hazra, Rohan Kapogiannis, Bill G. TI Preexposure Prophylaxis for Adolescents and Young Adults at Risk for HIV Infection: Is an Ounce of Prevention Worth a Pound of Cure? SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE HIV prevention; adolescents and young adults; preexposure prophylaxis; PrEP ID BONE-MINERAL DENSITY; UNITED-STATES; COST-EFFECTIVENESS; VACCINE TRIALS; MEN; CHALLENGES; YOUTH; SEX; INTERVENTIONS; RECOMMENDATIONS AB An alarming proportion of incident human immunodeficiency virus (HIV) infections worldwide occur in youth. In the United States, 69% of all new infections among youth occurred in young men who have sex with men (YMSM). Recent studies show the promise of preexposure prophylaxis (PrEP) for preventing HIV infection, but research efforts suffer from disproportionately low representation of the youth who are most at risk. Youth-focused research is critical and should include behavioral, community, and biomedical interventions to create a comprehensive HIV prevention package. The many ethical, legal, and regulatory considerations in conducting HIV research among, and in providing care services to, youth must be addressed so that those at high risk and most likely to benefit can have unfettered access to safe and effective health-promoting interventions. YMSM and minority youth are at substantial HIV risk and urgently need effective HIV prevention tools for which the short and long-term benefits and risks have been carefully considered. C1 [Pace, Jill E.; Siberry, George K.; Hazra, Rohan; Kapogiannis, Bill G.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Adolescent & Maternal AIDS Branch, NIH, Bethesda, MD 20892 USA. RP Kapogiannis, BG (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Ctr Res Mothers & Children, Pediat Adolescent & Maternal AIDS Branch, NIH, 6100 Execut Blvd,Rm 4B11J, Bethesda, MD 20892 USA. EM kapogiannisb@mail.nih.gov NR 42 TC 11 Z9 11 U1 1 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD APR 15 PY 2013 VL 56 IS 8 BP 1149 EP 1155 DI 10.1093/cid/cis1020 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 113WM UT WOS:000316700100019 PM 23223604 ER PT J AU Jaaro-Peled, H Niwa, M Foss, CA Murai, R de los Reyes, S Kamiya, A Mateo, Y O'Donnell, P Cascella, NG Nabeshima, T Guilarte, TR Pomper, MG Sawa, A AF Jaaro-Peled, Hanna Niwa, Minae Foss, Catherine A. Murai, Rina de los Reyes, Samantha Kamiya, Atsushi Mateo, Yolanda O'Donnell, Patricio Cascella, Nicola G. Nabeshima, Toshitaka Guilarte, Tomas R. Pomper, Martin G. Sawa, Akira TI Subcortical dopaminergic deficits in a DISC1 mutant model: a study in direct reference to human molecular brain imaging SO HUMAN MOLECULAR GENETICS LA English DT Article ID MAJOR DEPRESSION; TRANSGENIC MICE; BEHAVIORAL PHENOTYPES; GREATER AVAILABILITY; SCHIZOPHRENIA; AMPHETAMINE; TRANSPORTER; DISORDERS; RECEPTORS; RELEASE AB Imaging of the human brain has been an invaluable aid in understanding neuropsychopharmacology and, in particular, the role of dopamine in the striatum in mental illness. Here, we report a study in a genetic mouse model for major mental illness guided by results from human brain imaging: a systematic study using small animal positron emission tomography (PET), autoradiography, microdialysis and molecular biology in a putative dominant-negative mutant DISC1 transgenic model. This mouse model showed augmented binding of radioligands to the dopamine D2 receptor (D2R) in the striatum as well as neurochemical and behavioral changes to methamphetamine administration. Previously we reported that this model displayed deficits in the forced swim test, a representative indicator of antidepressant efficacy. By combining the results of our two studies, we propose a working hypothesis for future studies that this model might represent a mixed condition of depression and psychosis. We hope that this study will also help bridge a major gap in translational psychiatry between basic characterization of animal models and clinico-pharmacological assessment of patients mainly through PET imaging. C1 [Jaaro-Peled, Hanna; Niwa, Minae; de los Reyes, Samantha; Kamiya, Atsushi; Cascella, Nicola G.; Pomper, Martin G.; Sawa, Akira] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21287 USA. [Foss, Catherine A.; Pomper, Martin G.] Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21287 USA. [Niwa, Minae; Murai, Rina; Nabeshima, Toshitaka] Meijo Univ, Dept Chem Pharmacol, Nagoya, Aichi, Japan. [Mateo, Yolanda] NIAAA, Lab Integrat Neurosci, NIH, Rockville, MD 20852 USA. [O'Donnell, Patricio] Univ Maryland, Dept Anat & Neurobiol, Baltimore, MD 21201 USA. [Guilarte, Tomas R.] Johns Hopkins Univ, Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA. RP Sawa, A (reprint author), Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21287 USA. EM asawa1@jhmi.edu RI Niwa, Minae/G-5469-2011; kamiya, atsushi/L-8550-2016 OI Niwa, Minae/0000-0002-8784-7815; FU Stanley postdoctoral fellowship; NARSAD; NIH [MH-084018, MH-94268, MH-069853, MH-085226, MH-088753, MH-092443]; Stanley; RUSK; S-R foundations; Maryland Stem Cell Research Fund FX This work was supported by Stanley postdoctoral fellowship and NARSAD young investigator award (H.J.-P.), and by NIH grant numbers MH-084018, MH-94268 Silvo O. Conte center, MH-069853, MH-085226, MH-088753, MH-092443; Stanley; RUSK; S-R foundations; NARSAD; and Maryland Stem Cell Research Fund (A.S.). NR 40 TC 21 Z9 21 U1 2 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 15 PY 2013 VL 22 IS 8 BP 1574 EP 1580 DI 10.1093/hmg/ddt007 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 117PK UT WOS:000316965400008 PM 23314019 ER PT J AU Ganesh, SK Tragante, V Guo, W Guo, YR Lanktree, MB Smith, EN Johnson, T Castillo, BA Barnard, J Baumert, J Chang, YPC Elbers, CC Farrall, M Fischer, ME Franceschini, N Gaunt, TR Gho, JMIH Gieger, C Gong, Y Isaacs, A Kleber, ME Leach, IM McDonough, CW Meijs, MFL Mellander, O Molony, CM Nolte, IM Padmanabhan, S Price, TS Rajagopalan, R Shaffer, J Shah, S Shen, HQ Soranzo, N van der Most, PJ Van Iperen, EPA Van Setten, JA Vonk, JM Zhang, L Beitelshees, AL Berenson, GS Bhatt, DL Boer, JMA Boerwinkle, E Burkley, B Burt, A Chakravarti, A Chen, W Cooper-DeHoff, RM Curtis, SP Dreisbach, A Duggan, D Ehret, GB Fabsitz, RR Fornage, M Fox, E Furlong, CE Gansevoort, RT Hofker, MH Hovingh, GK Kirkland, SA Kottke-Marchant, K Kutlar, A LaCroix, AZ Langaee, TY Li, YR Lin, HH Liu, K Maiwald, S Malik, R Murugesan, G Newton-Cheh, C OConnell, JR Onland-Moret, NC Ouwehand, WH Palmas, W Penninx, BW Pepine, CJ Pettinger, M Polak, JF Ramachandran, VS Ranchalis, J Redline, S Ridker, PM Rose, LM Scharnag, H Schork, NJ Shimbo, D Shuldiner, AR Srinivasan, SR Stolk, RP Taylor, HA Thorand, B Trip, MD van Duijn, CM Verschuren, WM Wijmenga, C Winkelmann, BR Wyatt, S Young, JH Boehm, BO Caulfield, MJ Chasman, DI Davidson, KW Doevendans, PA FitzGerald, GA Gums, JG Hakonarson, H Hillege, HL Illig, T Jarvik, GP Johnson, JA Kastelein, JJP Koenig, W Marz, W Mitchell, BD Murray, SS Oldehinkel, AJ Rader, DJ Reilly, MP Reiner, AP Schadt, EE Silverstein, RL Snieder, H Stanton, AV Uitterlinden, AG van der Harst, P van der Schouw, YT Samani, NJ Johnson, AD Munroe, PB de Bakker, PIW Zhu, XF Levy, D Keating, BJ Asselbergs, FW AF Ganesh, Santhi K. Tragante, Vinicius Guo, Wei Guo, Yiran Lanktree, Matthew B. Smith, Erin N. Johnson, Toby Castillo, Berta Almoguera Barnard, John Baumert, Jens Chang, Yen-Pei Christy Elbers, Clara C. Farrall, Martin Fischer, Mary E. Franceschini, Nora Gaunt, Tom R. Gho, Johannes M. I. H. Gieger, Christian Gong, Yan Isaacs, Aaron Kleber, Marcus E. Leach, Irene Mateo McDonough, Caitrin W. Meijs, Matthijs F. L. Mellander, Olle Molony, Cliona M. Nolte, Ilja M. Padmanabhan, Sandosh Price, Tom S. Rajagopalan, Ramakrishnan Shaffer, Jonathan Shah, Sonia Shen, Haiqing Soranzo, Nicole van der Most, Peter J. Van Iperen, Erik P. A. Van Setten, Jessic A. Vonk, Judith M. Zhang, Li Beitelshees, Amber L. Berenson, Gerald S. Bhatt, Deepak L. Boer, Jolanda M. A. Boerwinkle, Eric Burkley, Ben Burt, Amber Chakravarti, Aravinda Chen, Wei Cooper-DeHoff, Rhonda M. Curtis, Sean P. Dreisbach, Albert Duggan, David Ehret, Georg B. Fabsitz, Richard R. Fornage, Myriam Fox, Ervin Furlong, Clement E. Gansevoort, Ron T. Hofker, Marten H. Hovingh, G. Kees Kirkland, Susan A. Kottke-Marchant, Kandice Kutlar, Abdullah LaCroix, Andrea Z. Langaee, Taimour Y. Li, Yun R. Lin, Honghuang Liu, Kiang Maiwald, Steffi Malik, Rainer Murugesan, Gurunathan Newton-Cheh, Christopher OConnell, Jeffery R. Onland-Moret, N. Charlotte Ouwehand, Willem H. Palmas, Walter Penninx, Brenda W. Pepine, Carl J. Pettinger, Mary Polak, Joseph F. Ramachandran, Vasan S. Ranchalis, Jane Redline, Susan Ridker, Paul M. Rose, Lynda M. Scharnag, Hubert Schork, Nicholas J. Shimbo, Daichi Shuldiner, Alan R. Srinivasan, Sathanur R. Stolk, Ronald P. Taylor, Herman A. Thorand, Barbara Trip, Mieke D. van Duijn, Cornelia M. Verschuren, W. Monique Wijmenga, Cisca Winkelmann, Bernhard R. Wyatt, Sharon Young, J. Hunter Boehm, Bernhard O. Caulfield, Mark J. Chasman, Daniel I. Davidson, Karina W. Doevendans, Pieter A. FitzGerald, Garret A. Gums, John G. Hakonarson, Hakon Hillege, Hans L. Illig, Thomas Jarvik, Gail P. Johnson, Julie A. Kastelein, John J. P. Koenig, Wolfgang Maerz, Winfried Mitchell, Braxton D. Murray, Sarah S. Oldehinkel, Albertine J. Rader, Daniel J. Reilly, Muredach P. Reiner, Alex P. Schadt, Eric E. Silverstein, Roy L. Snieder, Harold Stanton, Alice V. Uitterlinden, Andre G. van der Harst, Pim van der Schouw, Yvonne T. Samani, Nilesh J. Johnson, Andrew D. Munroe, Patricia B. de Bakker, Paul I. W. Zhu, Xiaofeng Levy, Daniel Keating, Brendan J. Asselbergs, Folkert W. CA CARDIOGRAM METASTROKE LifeLines Cohort Study TI Loci influencing blood pressure identified using a cardiovascular gene-centric array SO HUMAN MOLECULAR GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; HISTAMINE-RECEPTOR H-1; CORONARY-HEART-DISEASE; PULSE PRESSURE; EXPRESSION; P53; HYPERTENSION; MDM2; MICE; METAANALYSIS AB Blood pressure (BP) is a heritable determinant of risk for cardiovascular disease (CVD). To investigate genetic associations with systolic BP (SBP), diastolic BP (DBP), mean arterial pressure (MAP) and pulse pressure (PP), we genotyped 50 000 single-nucleotide polymorphisms (SNPs) that capture variation in 2100 candidate genes for cardiovascular phenotypes in 61 619 individuals of European ancestry from cohort studies in the USA and Europe. We identified novel associations between rs347591 and SBP (chromosome 3p25.3, in an intron of HRH1) and between rs2169137 and DBP (chromosome1q32.1 in an intron of MDM4) and between rs2014408 and SBP (chromosome 11p15 in an intron of SOX6), previously reported to be associated with MAP. We also confirmed 10 previously known loci associated with SBP, DBP, MAP or PP (ADRB1, ATP2B1, SH2B3/ATXN2, CSK, CYP17A1, FURIN, HFE, LSP1, MTHFR, SOX6) at array-wide significance (P 2.4 10(6)). We then replicated these associations in an independent set of 65 886 individuals of European ancestry. The findings from expression QTL (eQTL) analysis showed associations of SNPs in the MDM4 region with MDM4 expression. We did not find any evidence of association of the two novel SNPs in MDM4 and HRH1 with sequelae of high BP including coronary artery disease (CAD), left ventricular hypertrophy (LVH) or stroke. In summary, we identified two novel loci associated with BP and confirmed multiple previously reported associations. Our findings extend our understanding of genes involved in BP regulation, some of which may eventually provide new targets for therapeutic intervention. C1 [Ganesh, Santhi K.; Scharnag, Hubert] Univ Michigan Hlth Syst, Div Cardiovasc Med, Ann Arbor, MI USA. [Tragante, Vinicius; Gho, Johannes M. I. H.; Meijs, Matthijs F. L.; Doevendans, Pieter A.; Asselbergs, Folkert W.] Univ Med Ctr Utrecht, Dept Cardiol, Div Heart & Lungs, NL-3508 GA Utrecht, Netherlands. [Tragante, Vinicius; Elbers, Clara C.; Van Setten, Jessic A.; Onland-Moret, N. Charlotte; de Bakker, Paul I. W.; Asselbergs, Folkert W.] Univ Med Ctr Utrecht, Dept Med Genet, NL-3508 GA Utrecht, Netherlands. [Elbers, Clara C.; Onland-Moret, N. Charlotte; van der Schouw, Yvonne T.; de Bakker, Paul I. W.; Asselbergs, Folkert W.] Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, NL-3508 GA Utrecht, Netherlands. [Guo, Wei; Zhu, Xiaofeng] Case Western Reserve Univ, Sch Med, Dept Epidemiol & Biostat, Cleveland, OH 44106 USA. [Silverstein, Roy L.] Case Western Reserve Univ, Cleveland Clin Lerner Coll Med, Dept Mol Med, Cleveland, OH 44106 USA. [Guo, Yiran; Castillo, Berta Almoguera; Li, Yun R.; Hakonarson, Hakon; Keating, Brendan J.] Childrens Hosp Philadelphia, Ctr Appl Genom, Abramson Res Ctr, Philadelphia, PA 19104 USA. [Lanktree, Matthew B.] Univ Western Ontario, Dept Med, Schulich Sch Med & Dent, London, ON, Canada. [Lanktree, Matthew B.] Univ Western Ontario, Dept Biochem, Schulich Sch Med & Dent, London, ON, Canada. [Smith, Erin N.] Univ Calif San Diego, Sch Med, Dept Pediat, La Jolla, CA 92093 USA. [Smith, Erin N.] Univ Calif San Diego, Sch Med, Radys Childrens Hosp, La Jolla, CA 92093 USA. [Johnson, Toby; Caulfield, Mark J.; Munroe, Patricia B.] Queen Mary Univ London, London EC1M 6BQ, England. [Johnson, Toby; Caulfield, Mark J.; Munroe, Patricia B.] Queen Mary Univ London, Barts & London Genome Ctr, William Harvey Res Inst, London EC1M 6BQ, England. [Johnson, Toby; Munroe, Patricia B.] Queen Mary Univ London, Barts & London Sch Med & Dent, London EC1M 6BQ, England. [Barnard, John; Zhang, Li; Beitelshees, Amber L.] Cleveland Clin, Lerner Res Inst, Dept Quantitat Hlth Sci, Cleveland, OH 44106 USA. [Silverstein, Roy L.] Cleveland Clin, Lerner Res Inst, Dept Cell Biol, Cleveland, OH 44106 USA. [Baumert, Jens; Thorand, Barbara] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Epidemiol 2, Neuherberg, Germany. [Gieger, Christian] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Genet Epidemiol, Neuherberg, Germany. [Illig, Thomas] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Res Unit Mol Epidemiol, Neuherberg, Germany. [Chang, Yen-Pei Christy; Shen, Haiqing] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Chang, Yen-Pei Christy; OConnell, Jeffery R.; Shuldiner, Alan R.; Mitchell, Braxton D.] Univ Maryland, Sch Med, Div Endocrinol Diabet & Nutr, Baltimore, MD 21201 USA. [Farrall, Martin] Univ Oxford, Wellcome Trust Ctr Human Genet, Dept Cardiovasc Med, Oxford OX3 7BN, England. [Fischer, Mary E.] Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI USA. [Franceschini, Nora] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [Gaunt, Tom R.] Univ Bristol, Sch Social & Community Med, MRC Ctr Causal Anal Translat Epidemiol, Bristol BS8 2BN, Avon, England. [Gong, Yan; McDonough, Caitrin W.; Burkley, Ben; Cooper-DeHoff, Rhonda M.; Langaee, Taimour Y.; Gums, John G.; Johnson, Julie A.] Univ Florida, Dept Pharmacotherapy & Translat Res, Gainesville, FL USA. [Gong, Yan; McDonough, Caitrin W.; Burkley, Ben; Cooper-DeHoff, Rhonda M.; Langaee, Taimour Y.; Johnson, Julie A.] Univ Florida, Ctr Pharmacogen, Gainesville, FL USA. [Gums, John G.] Univ Florida, Dept Community Hlth & Family Med, Gainesville, FL USA. [Isaacs, Aaron; van Duijn, Cornelia M.] Erasmus MC, Dept Epidemiol, Genet Epidemiol Unit, Rotterdam, Netherlands. [Uitterlinden, Andre G.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Kleber, Marcus E.] LURIC Study Nonprofit LLC, Freiburg, Germany. [Kleber, Marcus E.; Maerz, Winfried] Heidelberg Univ, Mannheim Med Fac, Mannheim Inst Publ Hlth Social & Prevent Med, Mannheim, Germany. [Leach, Irene Mateo; Hillege, Hans L.; van der Harst, Pim] Univ Groningen, Univ Med Ctr Groningen, Dept Cardiol, Groningen, Netherlands. [Nolte, Ilja M.; van der Most, Peter J.; Vonk, Judith M.; Stolk, Ronald P.; Snieder, Harold] Univ Groningen, Univ Med Ctr Groningen, Dept Epidemiol, Groningen, Netherlands. [Gansevoort, Ron T.] Univ Groningen, Univ Med Ctr Groningen, Dept Med, Div Nephrol, Groningen, Netherlands. [Hofker, Marten H.] Univ Groningen, Univ Med Ctr Groningen, Med Biol Sect, Dept Pathol & Med Biol, Groningen, Netherlands. [Wijmenga, Cisca] Univ Groningen, Univ Med Ctr Groningen, Dept Genet, Groningen, Netherlands. [LifeLines Cohort Study] Univ Groningen, Univ Med Ctr Groningen, LifeLines Cohort Study, Groningen, Netherlands. [Oldehinkel, Albertine J.] Univ Groningen, Univ Med Ctr Groningen, Interdisciplinary Ctr Psychopathol & Emot Regulat, Groningen, Netherlands. [Mellander, Olle] Lund Univ, Dept Clin Sci, Malmo, Sweden. [Mellander, Olle] Skane Univ Hosp, Ctr Emergency Med, Malmo, Sweden. [Molony, Cliona M.] Rosetta Inpharmat, Dept Genet, Seattle, WA USA. [Padmanabhan, Sandosh] Univ Glasgow, BHF Glasgow Cardiovasc Res Ctr, Glasgow G12 8TA, Lanark, Scotland. [Price, Tom S.] Inst Psychiat, MRC SGDP Ctr, London, England. [Rajagopalan, Ramakrishnan; Burt, Amber; Furlong, Clement E.; Ranchalis, Jane; Jarvik, Gail P.] Univ Washington, Dept Med, Div Med Genet, Seattle, WA 98195 USA. [Shaffer, Jonathan; Palmas, Walter; Shimbo, Daichi; Davidson, Karina W.] Columbia Univ, Dept Med, New York, NY USA. [Shah, Sonia] UCL, UCL Genet Inst, Dept Genet Evolut & Environm, London WC1E 6BT, England. [Soranzo, Nicole] Univ Cambridge, Cambridge, England. [Ouwehand, Willem H.] Univ Cambridge, Dept Haematol, Cambridge, England. [Ouwehand, Willem H.] NHS Blood & Transplant, Cambridge, England. [Ouwehand, Willem H.] Wellcome Trust Sanger Inst, Hinxton, England. [Van Iperen, Erik P. A.; Asselbergs, Folkert W.] AMC, Durrer Ctr Cardiogenet Res, Amsterdam, Netherlands. [Van Iperen, Erik P. A.] AMC, Dept Clin Epidemiol Biostat & Bioinformat, Amsterdam, Netherlands. [Hovingh, G. Kees; Kastelein, John J. P.] AMC, Dept Vasc Med, Amsterdam, Netherlands. [Trip, Mieke D.] AMC, Dept Cardiol, Amsterdam, Netherlands. [Berenson, Gerald S.; Chen, Wei; Srinivasan, Sathanur R.] Tulane Univ, Dept Epidemiol, New Orleans, LA 70118 USA. [Bhatt, Deepak L.] Brigham & Womens Hosp, VA Boston Healthcare Syst, Boston, MA 02115 USA. [Bhatt, Deepak L.] Harvard Univ, Sch Med, Boston, MA USA. [Boer, Jolanda M. A.; Verschuren, W. Monique] Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands. [Boerwinkle, Eric; Fornage, Myriam] Univ Texas Hlth Sci Ctr, Ctr Human Genet, Houston, TX USA. [Boerwinkle, Eric; Fornage, Myriam] Univ Texas Hlth Sci Ctr, Inst Mol Med, Houston, TX USA. [Boerwinkle, Eric; Fornage, Myriam] Univ Texas Hlth Sci Ctr, Div Epidemiol, Houston, TX USA. Univ Texas Hlth Sci Ctr Houston, Houston, TX USA. [Chakravarti, Aravinda; Ehret, Georg B.] Johns Hopkins Univ, Sch Med, Ctr Complex Dis Genom, McKusick Nathans Inst Genet Med, Baltimore, MD USA. [Young, J. Hunter] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA. [Curtis, Sean P.] Merck Res Labs, Rahway, NJ 07065 USA. [Dreisbach, Albert; Fox, Ervin; Taylor, Herman A.] Univ Mississippi, Med Ctr, Dept Med, Jackson, MS 39216 USA. [Wyatt, Sharon] Univ Mississippi, Med Ctr, Sch Nursing, Jackson, MS 39216 USA. [Duggan, David] Translat Genom Res Inst, Phoenix, AZ USA. [Fabsitz, Richard R.; Pettinger, Mary] NHLBI, Div Cardiovasc Sci, Bethesda, MD 20892 USA. [Levy, Daniel] NHLBI, Ctr Populat Studies, Framingham, MA USA. [Kirkland, Susan A.] Dalhousie Univ, Dept Community Hlth & Epidemiol, Halifax, NS B3H 3J5, Canada. [Kottke-Marchant, Kandice; Murugesan, Gurunathan] Cleveland Clin, Pathol & Lab Med Inst, Cleveland, OH 44106 USA. [Kutlar, Abdullah] Med Coll Georgia, Augusta, GA 30912 USA. [LaCroix, Andrea Z.; Reiner, Alex P.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Lin, Honghuang; Ramachandran, Vasan S.] Boston Univ, Sch Med, Dept Med, Boston, MA 02118 USA. [Ramachandran, Vasan S.; Johnson, Andrew D.] NHLBI, Framingham Heart Study, Framingham, MA USA. [Liu, Kiang] Northwestern Univ, Feinberg Sch Med, Dept Prevent Med, Chicago, IL 60611 USA. [Maiwald, Steffi] Univ Amsterdam, Dept Vasc Med, Amsterdam, Netherlands. [Malik, Rainer] Univ Munich, Klinikum Grosshadern, Inst Stroke & Dementia Res, D-80539 Munich, Germany. [Malik, Rainer] Univ Munich, Klinikum Grosshadern, Neurol Klin, D-80539 Munich, Germany. [Newton-Cheh, Christopher; de Bakker, Paul I. W.] Broad Inst Harvard & MIT, Cambridge, MA USA. [Newton-Cheh, Christopher] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. [Penninx, Brenda W.] Vrije Univ Amsterdam Med Ctr, EMGO Inst, Dept Psychiat, Amsterdam, Netherlands. [Pepine, Carl J.] Univ Florida, Coll Med, Div Cardiovasc Med, Gainesville, FL USA. [Polak, Joseph F.] Tufts Med Ctr, Dept Radiol, Boston, MA USA. [Redline, Susan; Ridker, Paul M.; Rose, Lynda M.; Chasman, Daniel I.] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med, Boston, MA 02115 USA. [de Bakker, Paul I. W.] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Genet, Boston, MA 02115 USA. [Scharnag, Hubert] Med Univ Graz, Clin Inst Med & Chem Lab Diagnost, Graz, Austria. [Schork, Nicholas J.; Murray, Sarah S.] Scripps Translat Sci Inst, La Jolla, CA USA. [Schork, Nicholas J.] Scripps Res Inst, La Jolla, CA 92037 USA. [Shuldiner, Alan R.] Vet Adm Med Ctr, Geriatr Res & Educ Clin Ctr, Baltimore, MD 21218 USA. [Winkelmann, Bernhard R.] Cardiol Grp, Frankfurt, Germany. [Boehm, Bernhard O.] Univ Ulm, Med Ctr, Div Endocrinol, D-89069 Ulm, Germany. [Koenig, Wolfgang] Univ Ulm, Med Ctr, Dept Internal Med Cardiol 1, D-89069 Ulm, Germany. [Davidson, Karina W.] Columbia Univ, Dept Psychiat, New York, NY USA. [FitzGerald, Garret A.] Univ Penn, Sch Med, Inst Translat Med & Therapeut, Philadelphia, PA 19104 USA. [Illig, Thomas] Hannover Med Sch, Hannover Unified Biobank, Hannover, Germany. [Maerz, Winfried] Synlab Acad, Mannheim, Germany. [Murray, Sarah S.] Scripps Hlth, La Jolla, CA USA. [Rader, Daniel J.; Reilly, Muredach P.] Univ Penn, Perelman Sch Med, Cardiovasc Inst, Philadelphia, PA 19104 USA. [Schadt, Eric E.] Mt Sinai Sch Med, Dept Genet & Genom Sci, New York, NY USA. [Stanton, Alice V.] Royal Coll Surgeons Ireland, Dublin 2, Ireland. [Samani, Nilesh J.] Univ Leicester, Dept Cardiovasc Sci, Leicester LE3 9QP, Leics, England. [Samani, Nilesh J.] Glenfield Gen Hosp, Leicester NIHR Biomed Res Unit Cardiovasc Dis, Leicester LE3 9QP, Leics, England. RP Keating, BJ (reprint author), Univ Penn, Dept Pediat, Off 1016 Abramson Bldg,3615 Civ Ctr Blvd, Philadelphia, PA 19104 USA. EM bkeating@mail.med.upenn.edu; f.w.asselbergs@umcutrecht.nl RI Jarvik, Gail/N-6476-2014; Gaunt, Tom/O-3918-2014; Johnson, Andrew/G-6520-2013; sebastianovitsch, stepan/G-8507-2013; Stanton, Alice/F-4697-2012; de Bakker, Paul/B-8730-2009; Shah, Sonia/N-7547-2013; Wijmenga, Cisca/D-2173-2009; van der Schouw, Yvonne/F-8327-2014; Thorand, Barbara/B-5349-2014; Guo, Yiran/H-4120-2011; Price, Thomas/B-7372-2008; EHRET, Georg/A-9532-2009; Boehm, Bernhard/F-8750-2015; Onland-Moret, N. Charlotte/G-9185-2011; Padmanabhan, Sandosh/S-3963-2016; OI Jarvik, Gail/0000-0002-6710-8708; Gaunt, Tom/0000-0003-0924-3247; de Bakker, Paul/0000-0001-7735-7858; Shah, Sonia/0000-0001-5860-4526; van der Schouw, Yvonne/0000-0002-4605-435X; Thorand, Barbara/0000-0002-8416-6440; Guo, Yiran/0000-0002-6549-8589; Price, Thomas/0000-0001-7356-2109; Gieger, Christian/0000-0001-6986-9554; Wijmenga, Cisca/0000-0002-5635-1614; van Iperen, Erik/0000-0001-7107-3168; Mitchell, Braxton/0000-0003-4920-4744; Ouwehand, Willem/0000-0002-7744-1790; Kleber, Marcus/0000-0003-0663-7275; EHRET, Georg/0000-0002-5730-0675; Lanktree, Matthew/0000-0002-5750-6286; Lin, Honghuang/0000-0003-3043-3942; Stanton, Alice/0000-0002-4961-165X; Johnson, Toby/0000-0002-5998-3270; Padmanabhan, Sandosh/0000-0003-3869-5808; Ramachandran, Vasan/0000-0001-7357-5970; Soranzo, Nicole/0000-0003-1095-3852 FU National Heart, Lung and Blood Institute; Broad Institute [N01-HC-65226] FX We thank the National Heart, Lung and Blood Institute and the research institutions, study investigators and field staff for their support in creating the CARe program for biomedical research. We also thank the study participants, without whom this endeavor would not have been possible. DNA samples were genotyped in part through the Broad Institute (N01-HC-65226). NR 63 TC 61 Z9 61 U1 1 U2 49 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 15 PY 2013 VL 22 IS 8 BP 1663 EP 1678 DI 10.1093/hmg/dds555 PG 16 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 117PK UT WOS:000316965400016 PM 23303523 ER PT J AU Rerks-Ngarm, S Paris, RM Chunsutthiwat, S Premsri, N Namwat, C Bowonwatanuwong, C Li, SYS Kaewkungkal, J Trichavaroj, R Churikanont, N de Souza, MS Andrews, C Francis, D Adams, E Flores, J Gurunathan, S Tartaglia, J O'Connell, RJ Eamsila, C Nitayaphan, S Ngauy, V Thongcharoen, P Kunasol, P Michael, NL Robb, ML Gilbert, PB Kim, JH AF Rerks-Ngarm, Supachai Paris, Robert M. Chunsutthiwat, Supamit Premsri, Nakorn Namwat, Chawetsan Bowonwatanuwong, Chureeratana Li, Shuying S. Kaewkungkal, Jaranit Trichavaroj, Rapee Churikanont, Nampueng de Souza, Mark S. Andrews, Charla Francis, Donald Adams, Elizabeth Flores, Jorge Gurunathan, Sanjay Tartaglia, Jim O'Connell, Robert J. Eamsila, Chirapa Nitayaphan, Sorachai Ngauy, Viseth Thongcharoen, Prasert Kunasol, Prayura Michael, Nelson L. Robb, Merlin L. Gilbert, Peter B. Kim, Jerome H. TI Extended Evaluation of the Virologic, Immunologic, and Clinical Course of Volunteers Who Acquired HIV-1 Infection in a Phase III Vaccine Trial of ALVAC-HIV and AIDSVAX B/E SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; CD8(+) T-CELLS; NEUTRALIZING ANTIBODIES; SIMIAN IMMUNODEFICIENCY; DISEASE PROGRESSION; CANARYPOX VACCINE; RHESUS-MONKEYS; IMMUNE CONTROL; GENITAL-TRACT; VIRAL LOAD AB Background. The Thai Phase III Trial of ALVAC-HIV and AIDSVAX B/E showed an estimated vaccine efficacy (VE) of 31% to prevent acquisition of human immunodeficiency virus (HIV). Here we evaluated the effect of vaccination on disease progression after infection. Methods. CD4(+) T-cell counts and HIV viral load (VL) were measured serially. The primary analysis evaluated vaccine efficacy (VEP) as the percent reduction (vaccine vs placebo) in cumulative probability of a primary composite endpoint of clinical and CD4(+) count components at prespecified time points after infection. Secondary analyses of biomarker-based endpoints were assessed using marginal mean and linear mixed models. Results. There were 61 endpoints in the modified intent-to-treat cohort (mITT; n = 114). There was no evidence for efficacy at 30, 42, 54, and 60 months in the mITT and per protocol (n = 90) cohorts. Estimated VEP (mITT) was15.8% (-21.9, 41.8) at 60 months postinfection. There was weak evidence of lower VL and higher CD4(+) count at 60 and 66 months in the vaccine group. Lower mucosal VL was observed among vaccine recipients, primarily in semen (P = .04). Conclusions. Vaccination did not affect the clinical course of HIV disease after infection. A potential vaccine effect on the genital mucosa warrants further study. Trial registration. Clinicaltrials.gov identifier: NCT00337181. C1 [Rerks-Ngarm, Supachai; Chunsutthiwat, Supamit; Premsri, Nakorn; Namwat, Chawetsan; Kunasol, Prayura] Minist Publ Hlth, Dept Dis Control, Bangkok, Thailand. [Paris, Robert M.; de Souza, Mark S.; Andrews, Charla; O'Connell, Robert J.; Ngauy, Viseth; Michael, Nelson L.; Robb, Merlin L.; Kim, Jerome H.] US Mil HIV Res Program, Bethesda, MD 20817 USA. [Bowonwatanuwong, Chureeratana] Chonburi Reg Hosp, Chon Buri, Thailand. [Li, Shuying S.; Gilbert, Peter B.] Fred Hutchinson Canc Res Ctr, Stat Ctr HIV AIDS Res & Prevent Vaccine, Seattle, WA 98104 USA. [Li, Shuying S.; Gilbert, Peter B.] Fred Hutchinson Canc Res Ctr, Div Infect Dis, Seattle, WA 98104 USA. [Kaewkungkal, Jaranit] Mahidol Univ, Fac Trop Med, Bangkok, Thailand. [Trichavaroj, Rapee; Churikanont, Nampueng; de Souza, Mark S.; Ngauy, Viseth] USAMC AFRIMS, Dept Retrovirol, Bangkok, Thailand. [Francis, Donald] Global Solut Infect Dis, San Francisco, CA USA. [Adams, Elizabeth; Flores, Jorge] NIAID, Div Aids, NIH, Bethesda, MD 20892 USA. [Gurunathan, Sanjay; Tartaglia, Jim] Sanofi Pasteur, Swiftwater, PA USA. [Thongcharoen, Prasert] Mahidol Univ, Siriraj Hosp, Fac Med, Bangkok 10700, Thailand. RP Paris, RM (reprint author), US Mil HIV Res Program, 6720-A Rockledge Dr,Ste 400, Bethesda, MD 20817 USA. EM rparis@hivresearch.org FU US Army Medical Research and Materiel Command (USAMRMC) [Y1-AI-2642-12]; National Institutes of Allergy and Infectious Diseases [Y1-AI-2642-12]; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc [W81XWH-07-2-0067]; US Department of Defense (DOD) [W81XWH-07-2-0067] FX These studies were supported in part by an Interagency Agreement Y1-AI-2642-12 between US Army Medical Research and Materiel Command (USAMRMC) and the National Institutes of Allergy and Infectious Diseases. In addition, this work was supported by a cooperative agreement (W81XWH-07-2-0067) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc, and the US Department of Defense (DOD). NR 46 TC 20 Z9 20 U1 0 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD APR 15 PY 2013 VL 207 IS 8 BP 1195 EP 1205 DI 10.1093/infdis/jis478 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 113WT UT WOS:000316700800003 PM 22837492 ER PT J AU Safronetz, D Strong, JE Feldmann, F Haddock, E Sogoba, N Brining, D Geisbert, TW Scott, DP Feldmann, H AF Safronetz, David Strong, James E. Feldmann, Friederike Haddock, Elaine Sogoba, Nafomon Brining, Douglas Geisbert, Thomas W. Scott, Dana P. Feldmann, Heinz TI A Recently Isolated Lassa Virus From Mali Demonstrates Atypical Clinical Disease Manifestations and Decreased Virulence in Cynomolgus Macaques SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article DE Lassa fever; pathogenesis; disease modeling; guinea pigs; non-human primates; West Africa ID DENDRITIC CELLS; FEVER; INFECTION; MONKEYS; RIBAVIRIN; VACCINE; MONOCYTES/MACROPHAGES; PATHOGENESIS; REPLICATION; EXPRESSION AB The virulence of Soromba-R, a Lassa virus strain recently isolated from southern Mali, was assessed in 2 animal models of Lassa fever: inbred strain 13 guinea pigs and cynomolgus macaques. In both models, the Malian isolate demonstrated tissue tropism and viral titers similar to those of historical Lassa virus isolates from Sierra Leone (Josiah) and Liberia (Z-132); however, the Soromba-R isolate was found to be less pathogenic, as determined by decreased mortality and prolonged time to euthanasia in macaques. Interestingly, in addition to the classic indicators of Lassa fever, Soromba-R infection presented with moderate to severe pulmonary manifestations in the macaque model. Analysis of host responses demonstrated increased immune activation in Soromba-R-infected macaques, particularly in neutrophil-activating or -potentiating proinflammatory cytokines or growth factors, including tumor necrosis factor alpha, macrophage inflammatory protein 1 alpha, interleukin 1 beta, and granulocyte colony-stimulating factor, as well as interleukin 5, which may be responsible for the decreased lethality and uncharacteristic clinical presentation. These results suggest that the strain of Lassa virus circulating in Mali might be less pathogenic than strains circulating in the historical region of endemicity and may result in an atypical presentation for Lassa fever, which could complicate clinical diagnosis. C1 [Safronetz, David; Haddock, Elaine; Feldmann, Heinz] NIAID, Virol Lab, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USA. [Feldmann, Friederike] NIAID, Off Operat & Management, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USA. [Brining, Douglas; Scott, Dana P.] NIAID, Rocky Mt Vet Branch, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USA. [Geisbert, Thomas W.] Univ Texas Med Branch, Galveston Natl Lab, Galveston, TX USA. [Geisbert, Thomas W.] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX USA. [Strong, James E.] Publ Hlth Agcy Canada, Natl Microbiol Lab, Special Pathogens Program, Winnipeg, MB, Canada. [Strong, James E.] Univ Manitoba, Dept Pediat & Child Hlth, Winnipeg, MB R3T 2N2, Canada. [Strong, James E.; Feldmann, Heinz] Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada. [Sogoba, Nafomon] Univ Bamako, Fac Med Pharm & Dent, Malaria Res & Training Ctr, Bamako, Mali. RP Feldmann, H (reprint author), RML, 903 S 4th St, Hamilton, MT USA. EM feldmannh@niaid.nih.gov FU Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health FX This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health. NR 41 TC 10 Z9 10 U1 0 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD APR 15 PY 2013 VL 207 IS 8 BP 1316 EP 1327 DI 10.1093/infdis/jit004 PG 12 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 113WT UT WOS:000316700800017 PM 23303805 ER PT J AU Aslan, H Dey, R Meneses, C Castrovinci, P Jeronimo, SMB Oliva, G Fischer, L Duncan, RC Nakhasi, HL Valenzuela, JG Kamhawi, S AF Aslan, Hamide Dey, Ranadhir Meneses, Claudio Castrovinci, Philip Jeronimo, Selma Maria Bezerra Oliva, Gaetano Fischer, Laurent Duncan, Robert C. Nakhasi, Hira L. Valenzuela, Jesus G. Kamhawi, Shaden TI A New Model of Progressive Visceral Leishmaniasis in Hamsters by Natural Transmission via Bites of Vector Sand Flies SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article DE visceral leishmaniasis; Leishmania; Lutzomyia longipalpis; sand fly; vector; transmission; bite; intracardiac ID CUTANEOUS LEISHMANIASIS; LUTZOMYIA-LONGIPALPIS; PROTECTS HAMSTERS; SALIVARY PROTEIN; IN-VIVO; DONOVANI; IMMUNITY; INFANTUM; IMMUNOSUPPRESSION; INFECTION AB Background. Visceral leishmaniasis (VL) is transmitted by sand flies. Protection of needle-challenged vaccinated mice was abrogated in vector-initiated cutaneous leishmaniasis, highlighting the importance of developing natural transmission models for VL. Methods. We used Lutzomyia longipalpis to transmit Leishmania infantum or Leishmania donovani to hamsters. Vector-initiated infections were monitored and compared with intracardiac infections. Body weights were recorded weekly. Organ parasite loads and parasite pick-up by flies were assessed in sick hamsters. Results. Vector-transmitted L. infantum and L. donovani caused >= 5-fold increase in spleen weight compared with uninfected organs and had geometric mean parasite loads (GMPL) comparable to intracardiac inoculation of 10(7)-10(8) parasites, although vector-initiated disease progression was slower and weight loss was greater. Only vector-initiated L. infantum infections caused cutaneous lesions at transmission and distal sites. Importantly, 45.6%, 50.0%, and 33.3% of sand flies feeding on ear, mouth, and testicular lesions, respectively, were parasite-positive. Successful transmission was associated with a high mean percent of metacyclics (66%-82%) rather than total GMPL (2.0 x 10(4)-8.0 x 10(4)) per midgut. Conclusions. This model provides an improved platform to study initial immune events at the bite site, parasite tropism, and pathogenesis and to test drugs and vaccines against naturally acquired VL. C1 [Aslan, Hamide; Meneses, Claudio; Castrovinci, Philip; Valenzuela, Jesus G.; Kamhawi, Shaden] NIAID, VMBS, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 USA. [Dey, Ranadhir; Duncan, Robert C.; Nakhasi, Hira L.] US FDA, Div Emerging & Transfus Transmitted Dis, Ctr Biol Evaluat & Res, Bethesda, MD 20014 USA. [Jeronimo, Selma Maria Bezerra] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, BR-59072970 Natal, RN, Brazil. [Oliva, Gaetano] Univ Naples Federico II, Dept Vet Clin Sci, Naples, Italy. [Fischer, Laurent] MERIAL, Lyon, France. RP Kamhawi, S (reprint author), NIAID, VMBS, Lab Malaria & Vector Res, NIH, 12735 Twinbrook Pkwy,Rm 2E-22, Rockville, MD 20852 USA. EM skamhawi@niaid.nih.gov RI Jeronimo, Selma/M-8672-2014; Duncan, Robert/I-8168-2015 OI Duncan, Robert/0000-0001-8409-2501 FU Intramural Research Program of the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health; Intramural Research Program of the Office of Blood Research and Review, Center for Biologics Research and Review, US Food and Drug Administration FX The study was funded by the Intramural Research Program of the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health and by the Intramural Research Program of the Office of Blood Research and Review, Center for Biologics Research and Review, US Food and Drug Administration. Because R. D., R. C. D., H. L. N., J. G. V., and S. K. are government employees and this is government work, the work is in the public domain in the United States. Notwithstanding any other agreements, the NIH reserves the right to provide the work to PubMed Central for display and use by the public, and PubMed Central may tag or modify the work consistent with its customary practices. You can establish rights outside of the U. S. subject to a government use license. NR 36 TC 19 Z9 19 U1 0 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD APR 15 PY 2013 VL 207 IS 8 BP 1328 EP 1338 DI 10.1093/infdis/jis932 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 113WT UT WOS:000316700800018 PM 23288926 ER PT J AU Platt, RW Brookhart, MA Cole, SR Westreich, D Schisterman, EF AF Platt, Robert W. Brookhart, M. Alan Cole, Stephen R. Westreich, Daniel Schisterman, Enrique F. TI An information criterion for marginal structural models SO STATISTICS IN MEDICINE LA English DT Article DE bias; causal inference; marginal structural model; regression analysis; model specification ID POTENT ANTIRETROVIRAL THERAPY; LARGE RANDOMIZED-TRIAL; CAUSAL INFERENCE; INVERSE PROBABILITY; TREATMENT REGIMES; SURVIVAL ANALYSIS; TIME; ASSOCIATION; ASSUMPTION; DEFINITION AB Marginal structural models were developed as a semiparametric alternative to the G-computation formula to estimate causal effects of exposures. In practice, these models are often specified using parametric regression models. As such, the usual conventions regarding regression model specification apply. This paper outlines strategies for marginal structural model specification and considerations for the functional form of the exposure metric in the final structural model. We propose a quasi-likelihood information criterion adapted from use in generalized estimating equations. We evaluate the properties of our proposed information criterion using a limited simulation study. We illustrate our approach using two empirical examples. In the first example, we use data from a randomized breastfeeding promotion trial to estimate the effect of breastfeeding duration on infant weight at 1year. In the second example, we use data from two prospective cohorts studies to estimate the effect of highly active antiretroviral therapy on CD4 count in an observational cohort of HIV-infected men and women. The marginal structural model specified should reflect the scientific question being addressed but can also assist in exploration of other plausible and closely related questions. In marginal structural models, as in any regression setting, correct inference depends on correct model specification. Our proposed information criterion provides a formal method for comparing model fit for different specifications. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Platt, Robert W.] McGill Univ, Dept Epidemiol Biostat & Occupat Hlth, Montreal, PQ, Canada. [Brookhart, M. Alan; Cole, Stephen R.; Westreich, Daniel] Univ N Carolina, Dept Epidemiol, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Westreich, Daniel] Duke Univ, Dept Obstet & Gynecol, Durham, NC USA. [Westreich, Daniel] Duke Univ, Global Hlth Inst, Durham, NC USA. [Schisterman, Enrique F.] NICHD, Div Epidemiol Stat & Prevent Res, NIH, Bethesda, MD USA. RP Platt, RW (reprint author), McGill Univ, Dept Epidemiol Biostat & Occupat Hlth, Montreal, PQ, Canada. EM robert.platt@mcgill.ca OI Westreich, Daniel/0000-0003-3069-1650; Platt, Robert/0000-0002-5981-8443; Schisterman, Enrique/0000-0003-3757-641X FU Fonds de la Recherche en Sante du Quebec (FRSQ); FRSQ; Canadian Network for Observational Drug Effect Studies (CNODES); NIH [R01-AA-01759, AG027400]; NIH/NICHD [R00-HD-06-3961]; Eunice Kennedy Shriver National Institute of Child Health and Human Development; Thrasher Research Fund; National Health Research and Development Program (Health Canada); United Nations Children's Fund; European Regional Office of the World Health Organization; National Institute of Allergy and Infectious Diseases [UO1-AI-35004, UO1-AI-31834, UO1-AI-34994, UO1-AI-34989, UO1-AI-34993, UO1-AI-42590]; National Institute of Child Health and Human Development [UO1-HD-32632]; National Cancer Institute, the National Institute on Drug Abuse; National Institute on Deafness and Other Communication Disorders; National Institute of Allergy and Infectious Diseases; National Cancer Institute [UO1-AI-35042, UL1-RR025005 (GCRC), UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, UO1-AI-35041] FX Dr. Platt was supported by a Chercheur-Boursier (Research Scholar) award from the Fonds de la Recherche en Sante du Quebec (FRSQ), by core support to the McGill University Health Centre Research Institute from the FRSQ, and by the Canadian Network for Observational Drug Effect Studies (CNODES). Dr. Cole was supported in part by NIH grant R01-AA-01759. Dr. Brookhart is supported by a career development award from NIH (AG027400). Dr. Westreich received support from NIH/NICHD R00-HD-06-3961. Dr Schisterman is supported by the intramural program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development.; The authors thank Dr Michael Kramer for access to the PROBIT data. PROBIT was supported by grants from the Thrasher Research Fund, the National Health Research and Development Program (Health Canada), the United Nations Children's Fund, and the European Regional Office of the World Health Organization. The WIHS is funded by the National Institute of Allergy and Infectious Diseases (UO1-AI-35004, UO1-AI-31834, UO1-AI-34994, UO1-AI-34989, UO1-AI-34993, and UO1-AI-42590) and by the National Institute of Child Health and Human Development (UO1-HD-32632). The WIHS is co-funded by the National Cancer Institute, the National Institute on Drug Abuse, and the National Institute on Deafness and Other Communication Disorders. The MACS is funded by the National Institute of Allergy and Infectious Diseases, with additional supplemental funding from the National Cancer Institute (UO1-AI-35042, UL1-RR025005 (GCRC), UO1-AI-35043, UO1-AI-35039, UO1-AI-35040, and UO1-AI-35041). NR 55 TC 11 Z9 11 U1 0 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0277-6715 J9 STAT MED JI Stat. Med. PD APR 15 PY 2013 VL 32 IS 8 BP 1383 EP 1393 DI 10.1002/sim.5599 PG 11 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA 112WS UT WOS:000316625600012 PM 22972662 ER PT J AU Simonyan, K Herscovitch, P Horwitz, B AF Simonyan, Kristina Herscovitch, Peter Horwitz, Barry TI Speech-induced striatal dopamine release is left lateralized and coupled to functional striatal circuits in healthy humans: A combined PET, fMRI and DTI study SO NEUROIMAGE LA English DT Article DE Dopamine; Striatum; Speech functional networks; Structural networks ID DEEP BRAIN-STIMULATION; POSITRON-EMISSION-TOMOGRAPHY; BASAL GANGLIA; PARKINSONS-DISEASE; IN-VIVO; SPASMODIC DYSPHONIA; SOCIAL-CONTEXT; SUPRAGLOTTIC DYSTONIA; SUBTHALAMIC NUCLEUS; CONTINUOUS-INFUSION AB Considerable progress has been recently made in understanding the brain mechanisms underlying speech and language control. However, the neurochemical underpinnings of normal speech production remain largely unknown. We investigated the extent of striatal endogenous dopamine release and its influences on the organization of functional striatal speech networks during production of meaningful English sentences using a combination of positron emission tomography (PET) with the dopamine D-2/D-3 receptor radioligand [C-11]raclopride and functional MRI (fMRI). In addition, we used diffusion tensor tractography (DTI) to examine the extent of dopaminergic modulatory influences on striatal structural network organization. We found that, during sentence production, endogenous dopamine was released in the ventromedial portion of the dorsal striatum, in both its associative and sensorimotor functional divisions. In the associative striatum, speech-induced dopamine release established a significant relationship with neural activity and influenced the left-hemispheric lateralization of striatal functional networks. In contrast, there were no significant effects of endogenous dopamine release on the lateralization of striatal structural networks. Our data provide the first evidence for endogenous dopamine release in the dorsal striatum during normal speaking and point to the possible mechanisms behind the modulatory influences of dopamine on the organization of functional brain circuits controlling normal human speech. (c) 2012 Elsevier Inc. All rights reserved. C1 [Simonyan, Kristina] Mt Sinai Sch Med, Dept Neurol, New York, NY 10029 USA. [Simonyan, Kristina] Mt Sinai Sch Med, Dept Otolaryngol, New York, NY 10029 USA. [Simonyan, Kristina] NINDS, Laryngeal & Speech Sect, NIH, Bethesda, MD USA. [Herscovitch, Peter] NIH, PET Dept, Ctr Clin, Bethesda, MD 20892 USA. [Horwitz, Barry] Natl Inst Deafness & Other Commun Disorders, Brain Imaging & Modeling Sect, NIH, Bethesda, MD USA. RP Simonyan, K (reprint author), Mt Sinai Sch Med, Dept Neurol, 1 Gustave L Levy Pl,Box 1137, New York, NY 10029 USA. EM kristina.simonyan@mssm.edu OI Simonyan, Kristina/0000-0001-7444-0437 FU Intramural Programs of the National Institute of Neurological Disorders and Stroke; National Institute on Deafness and Other Communication Disorders; NIH Clinical Center; [DC009629] FX We would like to thank the PET Department of the NIH Clinical Center for assistance with PET data acquisition, Pamela Kearney, M.D., for subject evaluation, and Richard Reynolds, M.S., for help with data processing. Supported by DC009629 grant to KS, the Intramural Programs of the National Institute of Neurological Disorders and Stroke, the National Institute on Deafness and Other Communication Disorders, and the NIH Clinical Center. NR 107 TC 22 Z9 22 U1 0 U2 43 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 15 PY 2013 VL 70 BP 21 EP 32 DI 10.1016/j.neuroimage.2012.12.042 PG 12 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 100LX UT WOS:000315703800003 PM 23277111 ER PT J AU Ellegood, J Babineau, BA Henkelman, RM Lerch, JP Crawley, JN AF Ellegood, Jacob Babineau, Brooke A. Henkelman, R. Mark Lerch, Jason P. Crawley, Jacqueline N. TI Neuroanatomical analysis of the BTBR mouse model of autism using magnetic resonance imaging and diffusion tensor imaging SO NEUROIMAGE LA English DT Article DE BTBR T plus tf/J mice; Magnetic resonance imaging; Diffusion tensor imaging; Behavior ID T PLUS TF/J; CORPUS-CALLOSUM; ULTRASONIC VOCALIZATIONS; BEHAVIORAL PHENOTYPES; REPETITIVE BEHAVIOR; UNUSUAL REPERTOIRE; INBRED STRAINS; SCENT-MARKING; C57BL/6J MICE; SOCIAL BRAIN AB Autism is a neurodevelopmental disorder characterized by abnormal reciprocal social interactions, communication deficits, and repetitive behaviors with restricted interests. Autism-relevant phenotypes in the inbred mouse strain BTBR T + tf/J (BTBR) offer translational tools to discover biological mechanisms underlying unusual mouse behaviors analogous to symptoms of autism. Two of the most consistent findings with BTBR are lack of sociability as measured by the three-chamber social approach task and increased amount of time engaged in self-grooming in an empty cage. Here we evaluated BTBR as compared to two typical inbred strains with high sociability and low self-grooming, C57BL/6J (B6) and FVB/AntJ (FVB), on both the automated three-chambered social approach task and repetitive self-grooming assays. Brains from the behaviorally tested mice were analyzed using magnetic resonance imaging and diffusion tensor imaging to investigate potential neuroanatomical abnormalities throughout the brain; specifically, to discover neuroanatomical mechanisms which could explain the autism-relevant behavioral abnormalities. Significant differences in volume and white matter microstructure were detected in multiple anatomical regions throughout the brain of BTBR compared to B6 and FVB. Further, significant correlations were found between behavioral measures and areas of the brain known to be associated with those behaviors. For example, striatal volume was strongly correlated to time spent in self-grooming across strains. Our findings suggest that neuropathology exists in BTBR beyond the previously reported white matter abnormalities in the corpus callosum and hippocampal commissure and that these brain differences may be related to the behavioral abnormalities seen in BTBR. (C) 2013 Elsevier Inc. All rights reserved. C1 [Ellegood, Jacob; Henkelman, R. Mark; Lerch, Jason P.] Hosp Sick Children, Mouse Imaging Ctr, Toronto, ON M5T 3H7, Canada. [Henkelman, R. Mark; Lerch, Jason P.] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada. [Babineau, Brooke A.; Crawley, Jacqueline N.] NIMH, Bethesda, MD 20892 USA. RP Ellegood, J (reprint author), Hosp Sick Children, Mouse Imaging Ctr, 25 Orde St, Toronto, ON M5T 3H7, Canada. EM jacob@phenogenomics.ca RI Henkelman, Mark/F-3662-2011 FU Ontario Mental Health Foundation (OMHF); Canadian Institute for Health Research (CIHR); National Institute of Mental Health Intramural Research Program; Ontario Brain Institute (OBI); Ontario government FX We would like to thank Christine Laliberte for her assistance with the MRI scanning, Matthijs van Eede and Jan Scholz for help with different stages of the analysis, and Mu Yang and Jill Silverman for their assistance with the behavioral tasks and perfusions. We also acknowledge the Ontario Mental Health Foundation (OMHF) for salary support (Jacob Ellegood). This research was conducted with the support of the Canadian Institute for Health Research (CIHR), the National Institute of Mental Health Intramural Research Program, and the Ontario Brain Institute (OBI). OBI was created to become an internationally recognized centre of excellence in brain and neuroscience research. This independent non-profit corporation, funded partially by the Ontario government, is dedicated to improving approaches to the prevention, early diagnosis, treatment and management of neurological, and psychiatric disorders. The opinions, results, and conclusions are those of the authors and no endorsement by any of the agencies is intended or should be inferred. NR 85 TC 34 Z9 36 U1 1 U2 30 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 15 PY 2013 VL 70 BP 288 EP 300 DI 10.1016/j.neuroimage.2012.12.029 PG 13 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 100LX UT WOS:000315703800029 PM 23275046 ER PT J AU Perry, AS O'Hurley, G Raheem, OA Brennan, K Wong, S O'Grady, A Kennedy, AM Marignol, L Murphy, TM Sullivan, L Barrett, C Loftus, B Thornhill, J Hewitt, SM Lawler, M Kay, E Lynch, T Hollywood, D AF Perry, Antoinette S. O'Hurley, Gillian Raheem, Omer A. Brennan, Kevin Wong, Simon O'Grady, Anthony Kennedy, Anne-Marie Marignol, Laure Murphy, Therese M. Sullivan, Linda Barrett, Ciara Loftus, Barbara Thornhill, John Hewitt, Stephen M. Lawler, Mark Kay, Elaine Lynch, Thomas Hollywood, Donal TI Gene expression and epigenetic discovery screen reveal methylation of SFRP2 in prostate cancer SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE SFRP2; prostate cancer; hypermethylation; Wnt signaling ID PROTEIN-KINASE CK2; ANDROGEN RECEPTOR; WNT-ANTAGONIST; DNA METHYLATION; NEGATIVE REGULATOR; TUMOR PROGRESSION; BREAST-CANCER; BETA-CATENIN; CELL-GROWTH; FAMILY AB Aberrant activation of Wnts is common in human cancers, including prostate. Hypermethylation associated transcriptional silencing of Wnt antagonist genes SFRPs (Secreted Frizzled-Related Proteins) is a frequent oncogenic event. The significance of this is not known in prostate cancer. The objectives of our study were to (i) profile Wnt signaling related gene expression and (ii) investigate methylation of Wnt antagonist genes in prostate cancer. Using TaqMan Low Density Arrays, we identified 15 Wnt signaling related genes with significantly altered expression in prostate cancer; the majority of which were upregulated in tumors. Notably, histologically benign tissue from men with prostate cancer appeared more similar to tumor (r = 0.76) than to benign prostatic hyperplasia (BPH; r = 0.57, p < 0.001). Overall, the expression profile was highly similar between tumors of high ( 7) and low ( 6) Gleason scores. Pharmacological demethylation of PC-3 cells with 5-Aza-CdR reactivated 39 genes ( 2-fold); 40% of which inhibit Wnt signaling. Methylation frequencies in prostate cancer were 10% (2/20) (SFRP1), 64.86% (48/74) (SFRP2), 0% (0/20) (SFRP4) and 60% (12/20) (SFRP5). SFRP2 methylation was detected at significantly lower frequencies in high-grade prostatic intraepithelial neoplasia (HGPIN; 30%, (6/20), p = 0.0096), tumor adjacent benign areas (8.82%, (7/69), p < 0.0001) and BPH (11.43% (4/35), p < 0.0001). The quantitative level of SFRP2 methylation (normalized index of methylation) was also significantly higher in tumors (116) than in the other samples (HGPIN = 7.45, HB = 0.47, and BPH = 0.12). We show that SFRP2 hypermethylation is a common event in prostate cancer. SFRP2 methylation in combination with other epigenetic markers may be a useful biomarker of prostate cancer. C1 [Perry, Antoinette S.; Brennan, Kevin; Kennedy, Anne-Marie; Marignol, Laure; Murphy, Therese M.; Sullivan, Linda; Lawler, Mark; Hollywood, Donal] Trinity Coll Dublin, Inst Mol Med, Dept Clin Med, Dublin, Ireland. [O'Hurley, Gillian; O'Grady, Anthony; Kay, Elaine] RCSI ERC Beaumont Hosp, Dept Pathol, Dublin, Ireland. [Raheem, Omer A.; Lynch, Thomas] St James Hosp, Dept Urol, Dublin 8, Ireland. [Wong, Simon] Irish Ctr High End Comp, Dublin, Ireland. [Barrett, Ciara] St James Hosp, Dept Histopathol, Dublin 8, Ireland. [Loftus, Barbara] Adelaide & Meath Inc Natl Childrens Hosp, Dept Lab Med, Dublin, Ireland. [Thornhill, John] Adelaide & Meath Inc Natl Childrens Hosp, Dept Urol, Dublin, Ireland. [Hewitt, Stephen M.] NCI, Pathol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Perry, AS (reprint author), St James Hosp, Trinity Ctr Hlth Sci, Inst Mol Med, Dublin 8, Ireland. EM aperry@tcd.ie RI Kay, Elaine/D-8539-2013; Murphy, Therese/C-7481-2014; OI Murphy, Therese/0000-0002-7647-2798; Hewitt, Stephen/0000-0001-8283-1788; Perry, Antoinette/0000-0002-6108-512X; Marignol, Laure/0000-0002-2680-6200 FU Irish Cancer Society; Prostate Cancer Foundation FX Grant sponsors: the Irish Cancer Society, the Prostate Cancer Foundation NR 49 TC 13 Z9 15 U1 1 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2013 VL 132 IS 8 BP 1771 EP 1780 DI 10.1002/ijc.27798 PG 10 WC Oncology SC Oncology GA 090OE UT WOS:000314987800006 PM 22915211 ER PT J AU Gaiser, T Meinhardt, S Hirsch, D Killian, JK Gaedcke, J Jo, P Ponsa, I Miro, R Ruschoff, J Seitz, G Hu, Y Camps, J Ried, T AF Gaiser, Timo Meinhardt, Sandra Hirsch, Daniela Killian, Jonathan Keith Gaedcke, Jochen Jo, Peter Ponsa, Immaculada Miro, Rosa Rueschoff, Josef Seitz, Gerhard Hu, Yue Camps, Jordi Ried, Thomas TI Molecular patterns in the evolution of serrated lesion of the colorectum SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE serrated polyps; colorectal cancer; SSA/P; TSA; HP; MSI ID ISLAND METHYLATOR PHENOTYPE; COMPARATIVE GENOMIC HYBRIDIZATION; CANCER CELL-LINES; MICROSATELLITE INSTABILITY; COLON-CANCER; CHROMOSOMAL INSTABILITY; HYPERPLASTIC POLYPS; KRAS MUTATIONS; K-RAS; ADENOMAS AB Colorectal cancer (CRC) mostly develops from a variety of polyps following mainly three different molecular pathways: chromosomal instability (CIN), microsatellite instability (MSI) and CpG island methylation (CIMP). Polyps are classified histologically as conventional adenomas, hyperplastic polyps, sessile serrated adenomas/polyps (SSA/P) and traditional serrated adenomas (TSA). However, the association of these polyps with the different types of CRCs and the underlying genetic and epigenetic aberrations has yet to be resolved. In order to address this question we analyzed 140 tumors and 20 matched mucosae by array comparative genomic hybridization, by sequence analysis of the oncogenes BRAF, KRAS, PI3K3CA and by methylation arrays. MSI was tested indirectly by immunohistochemistry (IHC) and a loss of MLH1, MSH2, MSH6 or PMS2 was assigned as high microsatellite instability (MSI-H), while low microsatellite instability (MSI-L) was defined as MGMT IHC negativity only. CIN was detected in 78% of all MSI-H CRCs, most commonly as a gain of chromosome 8. Methylation data analyses allowed classification of samples into four groups and detected similar methylation profiles in SSA/P and MSI-H CRC. TSA also revealed aberrant methylation pattern, but clustered more heterogeneously and closer to microsatellite stable (MSS) CRCs. SSA/P, TSA and MSI-H CRCs had the highest degree of promotor methylation (CIMP pathway). Chromosomal instability, in contrast to the established doctrine, is a common phenomenon in MSI CRCs, yet to a lower extent and at later stages than in MSS CRCs. Methylation analyses suggest that SSA/P are precursors for MSI-H CRCs and follow the CIMP pathway. C1 [Gaiser, Timo; Meinhardt, Sandra; Hirsch, Daniela; Hu, Yue; Camps, Jordi; Ried, Thomas] NCI, Sect Canc Genom, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Gaiser, Timo] Heidelberg Univ, Med Fac Mannheim, Inst Pathol, Mannheim, Germany. [Killian, Jonathan Keith] NCI, Genet Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Gaedcke, Jochen; Jo, Peter] Univ Gottingen, Dept Gen & Visceral Surg, D-37073 Gottingen, Germany. [Ponsa, Immaculada; Miro, Rosa] Univ Autonoma Barcelona, Dept Biol Cellular Fisiol & Immunol, Bellaterra, Spain. [Ponsa, Immaculada; Miro, Rosa] Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Bellaterra, Spain. [Rueschoff, Josef] Inst Pathol Nordhessen, Kassel, Germany. [Seitz, Gerhard] Hosp Sozialstiftung Bamberg, Dept Pathol, Bamberg, Germany. RP Gaiser, T (reprint author), Heidelberg Univ, Med Fac Mannheim, Inst Pathol, Mannheim, Germany. EM timo.gaiser@umm.de FU Ministerio de Ciencia e Innovacion [SAF2007-64167]; Red Tematica de Investigacion Cooperativa en Cancer [RD06/0020/1020]; Generalitat de Catalunya, Spain [2009 SGR 1107]; RISE program of the German Academic Exchange Service (DAAD); Intramural Research Program of the NIH, National Cancer Institute (USA) FX Grant sponsor: Ministerio de Ciencia e Innovacion; Grant number: SAF2007-64167; Grant sponsor: Red Tematica de Investigacion Cooperativa en Cancer; Grant number: RD06/0020/1020; Grant sponsor: Generalitat de Catalunya, Spain; Grant number: 2009 SGR 1107; Grant sponsors: RISE program of the German Academic Exchange Service (DAAD), Intramural Research Program of the NIH, National Cancer Institute (USA) NR 49 TC 13 Z9 13 U1 0 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2013 VL 132 IS 8 BP 1800 EP 1810 DI 10.1002/ijc.27869 PG 11 WC Oncology SC Oncology GA 090OE UT WOS:000314987800009 PM 23011871 ER PT J AU Ly, D Forman, D Ferlay, J Brinton, LA Cook, MB AF Ly, Diana Forman, David Ferlay, Jacques Brinton, Louise A. Cook, Michael B. TI An international comparison of male and female breast cancer incidence rates SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE breast neoplasms; sex; incidence; trends; epidemiology; male; female ID BRCA2 MUTATION CARRIERS; RISK-FACTORS; UNITED-STATES; EPIDEMIOLOGY; MAMMOGRAPHY; SURVIVAL; PATTERNS; WOMEN; MEN; AGE AB Global international trends in female breast cancer incidence have been described previously but no comparable analysis of male breast cancer incidence rates has been conducted. We obtained male and female case and population data using Cancer Incidence in Five Continents (CI5). We calculated age-adjusted, sex-specific incidence rates and female-to-male incidence rate ratios (FMIRRs) and compared trends of such for the period 19882002. This analysis included 8,681 male breast cancer cases and 1.14 million female breast cancer cases. The highest male incidence rate was observed in Israel at 1.24 per 100,000 man-years, and the highest female incidence rate was observed in the United States at 90.7 per 100,000 woman-years. The lowest incidence rates for males (0.16) and females (18.0) were observed in Thailand. In general, male breast cancer incidence trends were variable; a minority of countries displayed evidence for an increase. In contrast, female incidence rates have been increasing in a majority of countries. The Pearson correlation coefficient (r) for male and female breast cancer incidence rates by country during 19882002 was 0.69. Male breast cancer rates were generally less than 1 per 100,000 man-years, in contrast to the much higher rates of female breast cancer, providing for an overall FMIRR of 122. The differences in both incidence rates and time trends between males and females may reflect sex differences in underlying risk factors, pathogenesis, and/or overdiagnosis. Conversely, the high correlation between male and female breast cancer incidences may indicate that both sexes share some common risk factors for breast cancer. C1 [Ly, Diana; Brinton, Louise A.; Cook, Michael B.] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Bethesda, MD 20852 USA. [Forman, David; Ferlay, Jacques] Int Agcy Res Canc, Sect Canc Informat, F-69372 Lyon 08, France. RP Cook, MB (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, DHHS, 6120 Execut Blvd,EPS Suite 550,Room 5014, Bethesda, MD 20852 USA. EM michael.cook@nih.gov RI Cook, Michael/A-5641-2009; Brinton, Louise/G-7486-2015 OI Cook, Michael/0000-0002-0533-7302; Brinton, Louise/0000-0003-3853-8562 FU National Cancer Institute, National Institutes of Health, Department of Health and Human Services FX Grant sponsors: Intramural Program of the National Cancer Institute, National Institutes of Health, Department of Health and Human Services NR 48 TC 28 Z9 30 U1 0 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2013 VL 132 IS 8 BP 1918 EP 1926 DI 10.1002/ijc.27841 PG 9 WC Oncology SC Oncology GA 090OE UT WOS:000314987800022 PM 22987302 ER PT J AU Ozarslan, E Shemesh, N Basser, PJ AF Oezarslan, Evren Shemesh, Noam Basser, Peter J. TI A general framework to quantify the effect of restricted diffusion on the NMR signal with applications to double pulsed field gradient NMR experiments (vol 130, pg 104702, 2009) SO JOURNAL OF CHEMICAL PHYSICS LA English DT Correction DE nuclear magnetic resonance C1 [Oezarslan, Evren; Basser, Peter J.] NICHD, Sect Tissue Biophys & Biomimet, NIH, Bethesda, MD 20892 USA. [Shemesh, Noam] Tel Aviv Univ, Sch Chem, Raymond & Beverly Sackler Fac Exact Sci, IL-69978 Ramat Aviv, Israel. RP Ozarslan, E (reprint author), Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Radiol, Boston, MA 02215 USA. EM evren@bwh.harvard.edu RI Ozarslan, Evren/B-4858-2013 OI Ozarslan, Evren/0000-0003-0859-1311 NR 3 TC 1 Z9 1 U1 2 U2 13 PU AMER INST PHYSICS PI MELVILLE PA CIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD APR 14 PY 2013 VL 138 IS 14 AR 149901 DI 10.1063/1.4801635 PG 1 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA 128SJ UT WOS:000317788800029 ER PT J AU Lattka, E Koletzko, B Zeilinger, S Hibbeln, JR Klopp, N Ring, SM Steer, CD AF Lattka, Eva Koletzko, Berthold Zeilinger, Sonja Hibbeln, Joseph R. Klopp, Norman Ring, Susan M. Steer, Colin D. TI Umbilical cord PUFA are determined by maternal and child fatty acid desaturase (FADS) genetic variants in the Avon Longitudinal Study of Parents and Children (ALSPAC) SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE FADS; Fetal fatty acid supply; Cord blood; Avon Longitudinal Study of Parents and Children (ALSPAC) ID HUMAN-FETAL LIVER; HUMAN-PLACENTA; DOCOSAHEXAENOIC ACID; LINOLENIC ACIDS; ATOPIC DISEASE; BREAST-MILK; FISH-OIL; CLUSTER; PLASMA; BLOOD AB Fetal supply with long-chain PUFA (LC-PUFA) during pregnancy is important for brain growth and visual and cognitive development and is provided by materno-fetal placental transfer. We recently showed that maternal fatty acid desaturase (FADS) genotypes modulate the amounts of LC-PUFA in maternal blood. Whether FADS genotypes influence the amounts of umbilical cord fatty acids has not been investigated until now. The aim of the present study was to investigate the influence of maternal and child FADS genotypes on the amounts of LC-PUFA in umbilical cord venous plasma as an indicator of fetal fatty acid supply during pregnancy. A total of eleven cord plasma n-6 and n-3 fatty acids were analysed for association with seventeen FADS gene cluster SNP in over 2000 mothers and children from the Avon Longitudinal Study of Parents and Children. In a multivariable analysis, the maternal genotype effect was adjusted for the child genotype and vice versa to estimate which of the two has the stronger influence on cord plasma fatty acids. Both maternal and child FADS genotypes and haplotypes influenced amounts of cord plasma LC-PUFA and fatty acid ratios. Specifically, most analysed maternal SNP were associated with cord plasma levels of the precursor n-6 PUFA, whereas the child genotypes were mainly associated with more highly desaturated n-6 LC-PUFA. This first study on FADS genotypes and cord fatty acids suggests that fetal LC-PUFA status is determined to some extent by fetal fatty acid conversion. Associations of particular haplotypes suggest specific effects of SNP rs498793 and rs968567 on fatty acid metabolism. C1 [Lattka, Eva; Zeilinger, Sonja; Klopp, Norman] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Res Unit Mol Epidemiol, Neuherberg, Germany. [Koletzko, Berthold] Univ Munich, Med Ctr, Dr von Hauner Childrens Hosp, Dept Pediat, Munich, Germany. [Hibbeln, Joseph R.] NIAAA, NIH, Bethesda, MD USA. [Klopp, Norman] Hannover Med Sch, Hannover Unified Biobank, Hannover, Germany. [Ring, Susan M.] Univ Bristol, Sch Social & Community Med, Avon Longitudinal Study Parents & Children ALSPAC, Bristol, Avon, England. [Steer, Colin D.] Univ Bristol, Sch Social & Community Med, Ctr Child & Adolescent Hlth, Bristol, Avon, England. RP Lattka, E (reprint author), Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Res Unit Mol Epidemiol, Neuherberg, Germany. EM eva.lattka@helmholtz-muenchen.de RI Reischl, Eva/B-9311-2013 OI Reischl, Eva/0000-0003-4055-8060 FU UK Medical Research Council; Wellcome Trust; University of Bristol; Commission of the European Communities [FP7-212652]; 'Kompetenznetz Adipositas' ('Competence Network for Adiposity'); Federal Ministry of Education and Research [FKZ: 01GI0826]; Munich Center of Health Sciences; Bristol-Myers-Squibb Foundation, New York, NY, USA; National Oceanic and Atmospheric Administration (NOAA), USA FX We are extremely grateful to all the families who took part in the present study, the midwives for their help in recruiting them, and the whole ALSPAC team, which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists and nurses. In particular we thank Professor Jean Golding for very insightful and valuable contributions. This publication is the work of the authors and does not reflect the views of the ALSPAC executive. The UK Medical Research Council, the Wellcome Trust and the University of Bristol provide core support for ALSPAC. This research was specifically funded by the Commission of the European Communities, specific RTD (Research and Technological Development) Programme 'Quality of Life and Management of Living Resources', within the 7th Framework Programme NUTRIMENTHE, FP7-212652. Further support was granted by the 'Kompetenznetz Adipositas' ('Competence Network for Adiposity') funded by the Federal Ministry of Education and Research (FKZ: 01GI0826) and by the Munich Center of Health Sciences. B. K. is the recipient of a Freedom to Discover Award of the Bristol-Myers-Squibb Foundation, New York, NY, USA. C. D. S. was partly supported by the National Oceanic and Atmospheric Administration (NOAA), USA. NR 68 TC 20 Z9 24 U1 1 U2 24 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD APR 14 PY 2013 VL 109 IS 7 BP 1196 EP 1210 DI 10.1017/S0007114512003108 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 121IM UT WOS:000317236600004 PM 22877655 ER PT J AU Chen, SH Huang, P Wang, ZH Wang, Z Swierczewska, M Niu, G Cui, DX Chen, XY AF Chen, Shouhui Huang, Peng Wang, Zhihua Wang, Zhe Swierczewska, Magdalena Niu, Gang Cui, Daxiang Chen, Xiaoyuan TI Self-assembly of gold nanoparticles to silver microspheres as highly efficient 3D SERS substrates SO NANOSCALE RESEARCH LETTERS LA English DT Article DE Self-assembly; Gold nanoparticles; Silver microspheres; SERS ID MAGNETIC NANOPARTICLES; ENHANCEMENT; NANOCRYSTALS; ARRAY; AG AB Herein we report a simple, one-pot, surfactant-free synthesis of 3D Ag microspheres (AgMSs) in aqueous phase at room temperature. The 3D AgMSs act as supports to fix the gold nanoparticles (GNPs) in 3D space via the interaction between the carboxyl groups of GNPs and the Ag atoms of AgMSs. The ensemble of AgMSs@GNPs with high surface-enhanced Raman scattering (SERS) activity and sensitivity can be an ideal 3D substrate choice for practical SERS detection applications. The simple self-assembly strategy may be extended to other metallic materials with great potentials in SERS, catalysis, and photoelectronic devices. C1 [Chen, Shouhui; Cui, Daxiang] Shanghai Jiao Tong Univ, Natl Key Lab Nano Micro Fabricat Technol, Inst Micronano Sci & Technol,Minist Educ, Key Lab Thin Film & Microfabricat,Dept Bionano Sc, Shanghai 200240, Peoples R China. [Chen, Shouhui; Huang, Peng; Wang, Zhihua; Wang, Zhe; Swierczewska, Magdalena; Niu, Gang; Chen, Xiaoyuan] NIBIB, Lab Mol Imaging & Nanomed LOMIN, NIH, Bethesda, MD 20892 USA. RP Cui, DX (reprint author), Shanghai Jiao Tong Univ, Natl Key Lab Nano Micro Fabricat Technol, Inst Micronano Sci & Technol,Minist Educ, Key Lab Thin Film & Microfabricat,Dept Bionano Sc, Shanghai 200240, Peoples R China. EM dxcui@sjtu.edu.cn; chenx5@mail.nih.gov RI Huang, Peng/H-9985-2013; Huang, Peng/R-2480-2016 OI Huang, Peng/0000-0003-3651-7813 FU National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH); National Key Basic Research Program (973 Project) [2010CB933902, 2011CB933100]; National 863 Hi-tech Project [2007AA022004]; Important National Science & Technology Specific Projects [2009ZX10004-311]; National Natural Scientific Fund [81225010, 20771075, 20803040, 81028009]; New Century Excellent Talent of Ministry of Education of China [NCET-08-0350]; Shanghai Science and Technology Fund [10XD1406100] FX This work was supported in part by the Intramural Research Program (IRP), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), the National Key Basic Research Program (973 Project) (2010CB933902 and 2011CB933100), National 863 Hi-tech Project (2007AA022004), Important National Science & Technology Specific Projects (2009ZX10004-311), National Natural Scientific Fund (nos. 81225010, 20771075, 20803040, and 81028009), New Century Excellent Talent of Ministry of Education of China (NCET-08-0350), and Shanghai Science and Technology Fund (10XD1406100). NR 30 TC 5 Z9 5 U1 9 U2 96 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1931-7573 J9 NANOSCALE RES LETT JI Nanoscale Res. Lett. PD APR 12 PY 2013 VL 8 AR 168 DI 10.1186/1556-276X-8-168 PG 7 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied SC Science & Technology - Other Topics; Materials Science; Physics GA 142JK UT WOS:000318793300001 PM 23587323 ER PT J AU Ingvarsen, S Porse, A Erpicum, C Maertens, L Jurgensen, HJ Madsen, DH Melander, MC Gardsvoll, H Hoyer-Hansen, G Noel, A Holmbeck, K Engelholm, LH Behrendt, N AF Ingvarsen, Signe Porse, Astrid Erpicum, Charlotte Maertens, Ludovic Jurgensen, Henrik J. Madsen, Daniel H. Melander, Maria C. Gardsvoll, Henrik Hoyer-Hansen, Gunilla Noel, Agnes Holmbeck, Kenn Engelholm, Lars H. Behrendt, Niels TI Targeting a Single Function of the Multifunctional Matrix Metalloprotease MT1-MMP IMPACT ON LYMPHANGIOGENESIS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MEMBRANE TYPE-1 METALLOPROTEINASE; LYMPHATIC ENDOTHELIAL-CELLS; EXTRACELLULAR-MATRIX; GELATINASE-A; MT-MMP; MULTICENTRIC OSTEOLYSIS; COMPLEX-FORMATION; IV COLLAGENASE; CANCER-THERAPY; TUMOR-GROWTH AB The group of matrix metalloproteases (MMPs) is responsible for multiple processes of extracellular matrix remodeling in the healthy body but also for matrix and tissue destruction during cancer invasion and metastasis. The understanding of the contributions from each individual MMP, both in healthy and pathological events, has been complicated by the lack of specific inhibitors and the fact that some of the potent MMPs are multifunctional enzymes. These factors have also hampered the setup of therapeutic strategies targetingMMPactivity. A tempting target is the membrane-associated MT1-MMP, which has well-documented importance in matrix degradation but which takes part in more than one pathway in this regard. In this report, we describe the selective targeting of a single function of this enzyme by means of a specific monoclonal antibody against MT1-MMP, raised in an MT1-MMP knock-out mouse. The antibody blocks the enzyme ability to activate proMMP-2 without interfering with the collagenolytic function or the general proteolytic activity of MT1-MMP. Using this antibody, we have shown that the MT1-MMP-catalyzed activation of proMMP-2 is involved in the outgrowth of cultured lymphatic endothelial cells in a collagen matrix in vitro, as well as in lymphatic vessel sprouting assayed ex vivo. This is the first example of the complete inactivation of a single function of a multifunctional MMP and the use of this strategy to pursue its role. C1 [Madsen, Daniel H.; Melander, Maria C.; Gardsvoll, Henrik; Hoyer-Hansen, Gunilla; Engelholm, Lars H.; Behrendt, Niels] Rigshosp, Finsen Lab, BRIC, DK-2200 Copenhagen N, Denmark. [Ingvarsen, Signe] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen N, Denmark. [Erpicum, Charlotte; Maertens, Ludovic; Noel, Agnes] Univ Liege, Lab Tumor & Dev Biol, GIGA Canc, B-4000 Liege, Belgium. [Holmbeck, Kenn] NIDCR, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD 20892 USA. RP Behrendt, N (reprint author), Rigshosp, Finsen Lab, BRIC, Copenhagen Bioctr, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark. EM niels.behrendt@finsenlab.dk OI Madsen, Daniel Hargboel/0000-0002-3183-6201; Noel, Agnes/0000-0002-7670-6179; Engelholm, Lars/0000-0002-6616-1232 FU National Institutes of Health, NIDCR; Danish Cancer Society; Danish Medical Research Council; Danish Cancer Research Foundation; Lundbeck Foundation; Novo Nordisk Foundation; Danish National Research Foundation (Danish-Chinese Center for Proteases and Cancer); Fonds de la Recherche Scientifique - FNRS (F.R.S.-FNRS, Belgium); Foundation against Cancer (Foundation of Public Interest, Belgium); Plan National Cancer (Service Public Federal, Belgium); European Community [201279]; "Grosserer Alfred Nielsen og Hustrus" Foundation; Copenhagen University Hospital; University of Copenhagen, Faculty of Science FX This work was supported in part by the Intramural Research Program of the National Institutes of Health, NIDCR (to K. H.), the Danish Cancer Society, the Danish Medical Research Council, the Danish Cancer Research Foundation, the Lundbeck Foundation, the Novo Nordisk Foundation, the Danish National Research Foundation (Danish-Chinese Center for Proteases and Cancer), the Fonds de la Recherche Scientifique - FNRS (F.R.S.-FNRS, Belgium), the Foundation against Cancer (Foundation of Public Interest, Belgium), the Plan National Cancer (Service Public Federal, Belgium), the European Community's Seventh Framework Programme FP7/2007-2011 under Grant Agreement n 201279 (to N.B., G. H.-H., and A.N.), and the "Grosserer Alfred Nielsen og Hustrus" Foundation (to L. H. E.). The work was also supported by personal grants from Copenhagen University Hospital (to A. P., D. H. M., and H.J.J.), from the Lundbeck Foundation (to D. H. M.) and from the University of Copenhagen, Faculty of Science (to S.I.). NR 53 TC 24 Z9 27 U1 0 U2 10 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 12 PY 2013 VL 288 IS 15 BP 10195 EP 10204 DI 10.1074/jbc.M112.447169 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 125TU UT WOS:000317565000002 PM 23413031 ER PT J AU Li, Y Wong, K Walsh, K Gao, B Zang, MW AF Li, Yu Wong, Kimberly Walsh, Kenneth Gao, Bin Zang, Mengwei TI Retinoic Acid Receptor beta Stimulates Hepatic Induction of Fibroblast Growth Factor 21 to Promote Fatty Acid Oxidation and Control Whole-body Energy Homeostasis in Mice SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ACTIVATED PROTEIN-KINASE; IMPROVES INSULIN SENSITIVITY; ADAPTIVE THERMOGENESIS; LIPID-METABOLISM; TRANSGENIC MICE; HEPG2 CELLS; PPAR-ALPHA; ALL-TRANS; EXPRESSION; OBESITY AB Activation of retinoic acid receptor (RAR) with all-trans-retinoic acid (RA) ameliorates glucose intolerance and insulin resistance in obese mice. The recently discovered fibroblast growth factor 21 (FGF21) is a hepatocyte-derived hormone that restores glucose and lipid homeostasis in obesity-induced diabetes. However, whether hepatic RAR is linked to FGF21 in the control of lipid metabolism and energy homeostasis remains elusive. Here we identify FGF21 as a direct target gene of RAR beta. The gene transcription of Fgf21 is increased by the RAR agonist RA and by overexpression of RAR alpha and RAR beta, but it is unaffected by RAR gamma in HepG2 cells. Promoter deletion analysis characterizes a putative RA-responsive element (RARE) primarily located in the 5'-flanking region of the Fgf21 gene. Disruption of the RARE sequence abolishes RA responsiveness. In vivo adenoviral overexpression of RAR beta in the liver enhances production and secretion of FGF21, which in turn promotes hepatic fatty acid oxidation and ketogenesis and ultimately leads to increased energy expenditure in mice. The metabolic effects of RA or RAR beta are mimicked by FGF21 overexpression and largely abolished by FGF21 knockdown. Moreover, hepatic RAR beta is bound to the putative RAREs of the Fgf21 promoter in a fasting-inducible manner in vivo, which contributes to FGF21 induction and the metabolic adaptation to prolonged fasting. In addition to other nuclear receptors, such as peroxisome proliferator-activated receptor alpha and retinoic acid receptor-related receptor alpha, RAR may act as a novel component to induce hepatic FGF21 in the regulation of lipid metabolism. The hepatic RAR-FGF21 pathway may represent a potential drug target for treating metabolic disorders. C1 [Li, Yu; Wong, Kimberly; Walsh, Kenneth; Zang, Mengwei] Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Dept Med, Boston, MA 02118 USA. [Gao, Bin] NIAAA, Lab Liver Dis, Bethesda, MD 20892 USA. RP Li, Y (reprint author), Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Vasc Biol Sect,Dept Med, X725,650 Albany St, Boston, MA 02118 USA. EM leonli@bu.edu; mwzang1@bu.edu OI li, yu/0000-0001-6910-5933 FU National Institutes of Health [DK076942]; Robert Dawson Evans Junior Faculty Merit Award; Wing Tat Lee Award; Boston University CTSI subsidy fund [UL1RR025771] FX This work was supported, in whole or in part, by National Institutes of Health Grant DK076942. This work was also supported by the Robert Dawson Evans Junior Faculty Merit Award, the Wing Tat Lee Award (to M. Z.), and Boston University CTSI subsidy fund Grant UL1RR025771 (to Y. L.). NR 50 TC 24 Z9 28 U1 3 U2 13 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 12 PY 2013 VL 288 IS 15 BP 10490 EP 10504 DI 10.1074/jbc.M112.429852 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 125TU UT WOS:000317565000029 PM 23430257 ER PT J AU Bradburne, CE Verhoeven, AB Manyam, GC Chaudhry, SA Chang, EL Thach, DC Bailey, CL van Hoek, ML AF Bradburne, Christopher E. Verhoeven, Anne B. Manyam, Ganiraju C. Chaudhry, Saira A. Chang, Eddie L. Thach, Dzung C. Bailey, Charles L. van Hoek, Monique L. TI Temporal Transcriptional Response during Infection of Type II Alveolar Epithelial Cells with Francisella tularensis Live Vaccine Strain (LVS) Supports a General Host Suppression and Bacterial Uptake by Macropinocytosis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID INNATE IMMUNE-RESPONSE; MURINE MACROPHAGES; MICROARRAY ANALYSIS; RESPIRATORY CHALLENGE; PULMONARY INFECTION; TULAREMIA VACCINE; YERSINIA EFFECTOR; INDUCED APOPTOSIS; HUMAN MONOCYTES; SEVERE SEPSIS AB Pneumonic tularemia is caused by inhalation of Francisella tularensis, one of the most infectious microbes known. We wanted to study the kinetics of the initial and early interactions between bacterium and host cells in the lung. To do this, we examined the infection of A549 airway epithelial cells with the live vaccine strain (LVS) of F. tularensis. A549 cells were infected and analyzed for global transcriptional response at multiple time points up to 16 h following infection. At 15 min and 2 h, a strong transcriptional response was observed including cytoskeletal rearrangement, intracellular transport, and interferon signaling. However, at later time points (6 and 16 h), very little differential gene expression was observed, indicating a general suppression of the host response consistent with other reported cell lines and murine tissues. Genes for macropinocytosis and actin/cytoskeleton rearrangement were highly up-regulated and common to the 15 min and 2 h time points, suggesting the use of this method for bacterial entry into cells. We demonstrate macropinocytosis through the uptake of FITC-dextran and amiloride inhibition of Francisella LVS uptake. Our results suggest that macropinocytosis is a potential mechanism of intracellular entry by LVS and that the host cell response is suppressed during the first 2-6 h of infection. These results suggest that the attenuated Francisella LVS induces significant host cell signaling at very early time points after the bacteria's interaction with the cell. C1 [Bradburne, Christopher E.; Chang, Eddie L.; Thach, Dzung C.] USN, Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. [Bradburne, Christopher E.] Johns Hopkins Univ, Appl Phys Lab, Asymmetr Operat Dept, Laurel, MD 20723 USA. [Verhoeven, Anne B.; Manyam, Ganiraju C.; Chaudhry, Saira A.; van Hoek, Monique L.] George Mason Univ, Sch Syst Biol, Manassas, VA 20110 USA. [Manyam, Ganiraju C.] Univ Texas MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA. [Thach, Dzung C.] NIAID, NIH, Bethesda, MD 20892 USA. [Bailey, Charles L.; van Hoek, Monique L.] George Mason Univ, Natl Ctr Biodef & Infect Dis, Manassas, VA 20110 USA. RP van Hoek, ML (reprint author), George Mason Univ, Discovery Hall,MSN 1H8,10910 Univ Blvd, Manassas, VA 20110 USA. EM mvanhoek@gmu.edu RI Manyam, Ganiraju/B-5890-2014; Manyam, Ganiraju/E-9150-2012; OI Manyam, Ganiraju/0000-0001-6006-8961; van Hoek, Monique/0000-0003-1679-4899 FU Joint Science and Technology Office for Chemical and Biological Defense/Defense Threat Reduction Agency; U.S. Army Medical Research and Material Command [W81XWH-06-C-0360, HDTRA1-12-C-0039]; National Research Council FX This work was supported through the Joint Science and Technology Office for Chemical and Biological Defense/Defense Threat Reduction Agency and contracted through the U.S. Army Medical Research and Material Command under Contract No. W81XWH-06-C-0360 (to A. B. V., C. L. B., and M. V. H.) and HDTRA1-12-C-0039 (to M. V. H.).; Supported by a fellowship from the National Research Council while at the Center for Bio/Molecular Science and Engineering/United States Naval Research Laboratory and by internal funding from the Asymmetric Operations Department while at Johns Hopkins University/Applied Physics Laboratory. NR 79 TC 4 Z9 4 U1 0 U2 13 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 12 PY 2013 VL 288 IS 15 BP 10780 EP 10791 DI 10.1074/jbc.M112.362178 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 125TU UT WOS:000317565000056 PM 23322778 ER PT J AU Gray, GE Metch, B Churchyard, G Mlisana, K Nchabeleng, M Allen, M Moodie, Z Kublin, J Bekker, LG AF Gray, G. E. Metch, B. Churchyard, G. Mlisana, K. Nchabeleng, M. Allen, M. Moodie, Z. Kublin, J. Bekker, L. -G. CA HVTN 503 Team TI Does participation in an HIV vaccine efficacy trial affect risk behaviour in South Africa? SO VACCINE LA English DT Article DE HIV risk behaviour; HIV vaccine trials; Perception of risk; South Africa ID MALE CIRCUMCISION; DOUBLE-BLIND; PREVENTION; INFECTION; COMPENSATION; INTERVENTION; ACQUISITION AB Background: Increased sexual risk behaviour in participants enrolled in HIV prevention trials has been a concern. The HVTN 503/Phambili study, a phase 2B study of the Merck Ad-5 multiclade HIV vaccine in South Africa, suspended enrollment and vaccinations following the results of the Step study. Participants were notified of their treatment allocation and continue to be followed. We investigated changes in risk behaviour over time and assessed the impact of study unblinding. Methods: 801 participants were enrolled. Risk behaviours were assessed with an interviewer-administered questionnaire at 6-month intervals. We assessed change from enrolment to the first 6-month assessment pre-unblinding and between enrolment and at least 6 months post-unblinding on all participants with comparable data. A one-time unblinding risk perception questionnaire was administered post-unblinding. Results: A decrease in participants reporting unprotected sex was observed in both measured time periods for men and women, with no differences by treatment arm. At 6 months (pre-unblinding), 29.6% of men and 35.8% of women reported changing from unprotected to protected sex (p < 0.0001 for each). Men (22%) were more likely than women (14%) to report behaviour change after unblinding (p = 0.009). Post-enrolment, 142 (45%) of 313 previously uncircumcised men underwent medical circumcision. 663 participants completed the unblinding questionnaire. More vaccine (24.6%) as compared to placebo recipients (12.0%) agreed that they were more likely to get HIV than most people (p < 0.0001), and attributed this to receiving the vaccine. Conclusion: We did not find evidence of risk compensation during this clinical trial. Some risk behaviour reductions including male circumcision were noted irrespective of treatment allocation. (c) 2013 Published by Elsevier Ltd. C1 [Gray, G. E.] Univ Witwatersrand, Perinatal HIV Res Unit, ZA-2050 Johannesburg, South Africa. [Metch, B.; Moodie, Z.] Fred Hutchinson Canc Res Ctr, Stat Ctr HIV AIDS Res & Prevent, Seattle, WA 98104 USA. [Churchyard, G.] Aurum Inst, Johannesburg, South Africa. [Churchyard, G.] Univ Witwatersrand, Johannesburg, South Africa. [Mlisana, K.] Univ KwaZulu Natal, Ctr AIDS Programme Res South Africa, Durban, South Africa. [Nchabeleng, M.] Univ Limpopo, MEDUNSA HIV Clin Res Unit, Sovenga, South Africa. [Allen, M.] NIAID, Vaccine Res Program, Div Aids, NIH, Bethesda, MD USA. [Kublin, J.] Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, Seattle, WA 98104 USA. [Bekker, L. -G.] Univ Cape Town, Desmond Tutu HIV Ctr, IIDMM, ZA-7925 Cape Town, South Africa. [Bekker, L. -G.] Univ Cape Town, Dept Med, ZA-7925 Cape Town, South Africa. RP Bekker, LG (reprint author), Univ Cape Town, Inst Infect Dis & Mol Med, Desmond Tutu HIV Ctr, Wernher Beit Bldg,Anzio Rd Observ, ZA-7925 Cape Town, South Africa. EM Linda-gail.Bekker@hiv-research.org.za OI Allen, Mary/0000-0001-8163-0714; Mlisana, Koleka/0000-0002-8436-3268 FU NIAID NIH HHS [UM1 AI068635, UM1 AI068614] NR 20 TC 8 Z9 8 U1 0 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD APR 12 PY 2013 VL 31 IS 16 BP 2089 EP 2096 DI 10.1016/j.vaccine.2013.01.031 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 128WE UT WOS:000317799600014 PM 23370155 ER PT J AU Gullingsrud, J Saveria, T Amos, E Duffy, PE Oleinikov, AV AF Gullingsrud, Justin Saveria, Tracy Amos, Emily Duffy, Patrick E. Oleinikov, Andrew V. TI Structure-Function-Immunogenicity Studies of PfEMP1 Domain DBL2 beta(PF11_0521), a Malaria Parasite Ligand for ICAM-1 SO PLOS ONE LA English DT Article ID INTERCELLULAR-ADHESION MOLECULE-1; PLASMODIUM-FALCIPARUM; CRYSTAL-STRUCTURE; BINDING DOMAIN; IMMUNE EVASION; CYTOADHERENCE; RECEPTOR; PROTEIN; SEQUESTRATION; ERYTHROCYTES AB Plasmodium falciparum virulence has been ascribed to its ability to sequester in deep vascular beds, mediated by the variant surface antigen family PfEMP1 binding endothelial receptors like ICAM-1. We previously observed that naturally-acquired antibodies that block a PfEMP1 domain, DBL2 beta of PF11_0521 allele, from binding to the human ICAM1 receptor, reduce the risk of malaria hospitalization in children. Here, we find that DBL2 beta(PF11_0521) binds ICAM-1 in the low nM range and relate the structure of this domain with its function and immunogenicity. We demonstrate that the interaction with ICAM-1 is not impaired by point mutations in the N-terminal subdomain or in the flexible Loop 4 of DBL2 beta(PF11_0521), although both substructures were previously implicated in binding ICAM-1. These data will help to refine the existing model of DBL beta::ICAM-1 interactions. Antibodies raised against full-length DBL2 beta(PF11_0521), but not truncated forms lacking the N terminal fragment, block its interaction with ICAM-1. Our data suggest that full length domain is optimal for displaying functional epitopes and has a broad surface of interaction with ICAM-1 that is not disrupted by individual amino acid substitutions at putative key residues. This information might be important for the future design of anti-malarial vaccines based on PfEMP1 antigens. C1 [Gullingsrud, Justin; Saveria, Tracy; Amos, Emily; Duffy, Patrick E.; Oleinikov, Andrew V.] Seattle Biomed Res Inst, Seattle, WA 98109 USA. [Duffy, Patrick E.] Univ Washington, Program Pathobiol, Dept Global Hlth, Seattle, WA 98195 USA. [Duffy, Patrick E.] NIAID, Lab Malaria Immunol & Vaccinol, NIH, Rockville, MD USA. RP Oleinikov, AV (reprint author), Seattle Biomed Res Inst, 4 Nickerson St, Seattle, WA 98109 USA. EM andrew.oleinikov@sbri.org FU National Institutes of Health [1R21AI064503, 1R56AI083668, 1R01AI092120]; Foundation of the National Institutes of Health through the Grand Challenges in Global Health initiative [29202] FX The work was supported by National Institutes of Health (http://www.nih.gov) [grants 1R21AI064503, 1R56AI083668, and 1R01AI092120 to AVO], and funded in part by the Foundation of the National Institutes of Health through the Grand Challenges in Global Health initiative (http://www.grandchallenges.org/Pages/default.aspx) [Grant No. 29202 to PED]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 7 Z9 7 U1 0 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 12 PY 2013 VL 8 IS 4 AR e61323 DI 10.1371/journal.pone.0061323 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 123JP UT WOS:000317385300071 PM 23593462 ER PT J AU Jain, S Kapetanaki, MG Raghavachari, N Woodhouse, K Yu, GY Barge, S Coronnello, C Benos, PV Kato, GJ Kaminski, N Gladwin, MT AF Jain, Shilpa Kapetanaki, Maria G. Raghavachari, Nalini Woodhouse, Kimberly Yu, Guoying Barge, Suchitra Coronnello, Claudia Benos, Panayiotis V. Kato, Gregory J. Kaminski, Naftali Gladwin, Mark T. TI Expression of Regulatory Platelet MicroRNAs in Patients with Sickle Cell Disease SO PLOS ONE LA English DT Article ID PULMONARY-HYPERTENSION; RETICULATED PLATELETS; ERYTHROCYTE ADHERENCE; POTENTIAL ROLE; ACTIVATION; PROFILES; MICROPARTICLES; MECHANISMS; TARGETS; ANEMIA AB Background: Increased platelet activation in sickle cell disease (SCD) contributes to a state of hypercoagulability and confers a risk of thromboembolic complications. The role for post-transcriptional regulation of the platelet transcriptome by microRNAs (miRNAs) in SCD has not been previously explored. This is the first study to determine whether platelets from SCD exhibit an altered miRNA expression profile. Methods and Findings: We analyzed the expression of miRNAs isolated from platelets from a primary cohort (SCD = 19, controls = 10) and a validation cohort (SCD = 7, controls = 7) by hybridizing to the Agilent miRNA microarrays. A dramatic difference in miRNA expression profiles between patients and controls was noted in both cohorts separately. A total of 40 differentially expressed platelet miRNAs were identified as common in both cohorts (p-value 0.05, fold change>2) with 24 miRNAs downregulated. Interestingly, 14 of the 24 downregulated miRNAs were members of three families - miR-329, miR-376 and miR-154 - which localized to the epigenetically regulated, maternally imprinted chromosome 14q32 region. We validated the downregulated miRNAs, miR-376a and miR-409-3p, and an upregulated miR-1225-3p using qRT-PCR. Overexpression of the miR-1225-3p in the Meg01 cells was followed by mRNA expression profiling to identify mRNA targets. This resulted in significant transcriptional repression of 1605 transcripts. A combinatorial approach using Meg01 mRNA expression profiles following miR-1225-3p overexpression, a computational prediction analysis of miRNA target sequences and a previously published set of differentially expressed platelet transcripts from SCD patients, identified three novel platelet mRNA targets: PBXIP1, PLAGL2 and PHF20L1. Conclusions: We have identified significant differences in functionally active platelet miRNAs in patients with SCD as compared to controls. These data provide an important inventory of differentially expressed miRNAs in SCD patients and an experimental framework for future studies of miRNAs as regulators of biological pathways in platelets. C1 [Jain, Shilpa] Univ Pittsburgh, Childrens Hosp Pittsburgh, Med Ctr, Div Pediat Hematol Oncol, Pittsburgh, PA 15213 USA. [Jain, Shilpa; Barge, Suchitra; Gladwin, Mark T.] Univ Pittsburgh, Vasc Med Inst, Pittsburgh, PA USA. [Kapetanaki, Maria G.; Yu, Guoying; Kaminski, Naftali] Univ Pittsburgh, Sch Med, Div Pulm Allergy & Crit Care Med, Dorothy P & Richard P Simmons Ctr Interstitial Lu, Pittsburgh, PA 15260 USA. [Raghavachari, Nalini; Woodhouse, Kimberly] NHLBI, Genom Core Facil, NIH, Bethesda, MD 20892 USA. [Coronnello, Claudia; Benos, Panayiotis V.] Univ Pittsburgh, Sch Med, Dept Computat & Syst Biol, Pittsburgh, PA USA. [Coronnello, Claudia] Fdn Ri Med, Palermo, Italy. [Kato, Gregory J.] NHLBI, Sickle Cell Vasc Dis Sect, Pulm & Vasc Med Branch, NIH, Bethesda, MD 20892 USA. [Gladwin, Mark T.] Univ Pittsburgh, Sch Med, Div Pulm Allergy & Crit Care Med, Pittsburgh, PA USA. RP Kaminski, N (reprint author), Univ Pittsburgh, Sch Med, Div Pulm Allergy & Crit Care Med, Dorothy P & Richard P Simmons Ctr Interstitial Lu, Pittsburgh, PA 15260 USA. EM kaminskin@upmc.edu; gladwinmt@upmc.edu RI Kato, Gregory/I-7615-2014; OI Kato, Gregory/0000-0003-4465-3217; Benos, Panayiotis/0000-0003-3172-3132; Kaminski, Naftali/0000-0001-5917-4601 FU National Institutes of Health (NIH) [R01HL098032, RO1HL096973, PO1HL103455]; Institute for Transfusion Medicine; Hemophilia Center of Western Pennsylvania; NIH [R01LM009657, R01LM007994]; Fondazione RiMED Fellowship FX Dr. Mark Gladwin receives research support from National Institutes of Health (NIH) grants R01HL098032, RO1HL096973, and PO1HL103455, the Institute for Transfusion Medicine and the Hemophilia Center of Western Pennsylvania. Panayiotis V. Benos was supported by NIH grants R01LM009657 and R01LM007994. Claudia Coronnello was supported by a Fondazione RiMED Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 7 Z9 7 U1 0 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 12 PY 2013 VL 8 IS 4 AR e60932 DI 10.1371/journal.pone.0060932 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 123JP UT WOS:000317385300035 PM 23593351 ER PT J AU Grohman, JK Gorelick, RJ Lickwar, CR Lieb, JD Bower, BD Znosko, BM Weeks, KM AF Grohman, Jacob K. Gorelick, Robert J. Lickwar, Colin R. Lieb, Jason D. Bower, Brian D. Znosko, Brent M. Weeks, Kevin M. TI A Guanosine-Centric Mechanism for RNA Chaperone Function SO SCIENCE LA English DT Article ID MURINE LEUKEMIA-VIRUS; NUCLEOCAPSID PROTEIN; SHAPE CHEMISTRY; PARAMETERS; DUPLEXES; GENOME; PAIRS AB RNA chaperones are ubiquitous, heterogeneous proteins essential for RNA structural biogenesis and function. We investigated the mechanism of chaperone-mediated RNA folding by following the time-resolved dimerization of the packaging domain of a retroviral RNA at nucleotide resolution. In the absence of the nucleocapsid (NC) chaperone, dimerization proceeded through multiple, slow-folding intermediates. In the presence of NC, dimerization occurred rapidly through a single structural intermediate. The RNA binding domain of heterogeneous nuclear ribonucleoprotein A1 protein, a structurally unrelated chaperone, also accelerated dimerization. Both chaperones interacted primarily with guanosine residues. Replacing guanosine with more weakly pairing inosine yielded an RNA that folded rapidly without a facilitating chaperone. These results show that RNA chaperones can simplify RNA folding landscapes by weakening intramolecular interactions involving guanosine and explain many RNA chaperone activities. C1 [Grohman, Jacob K.; Weeks, Kevin M.] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA. [Grohman, Jacob K.] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA. [Lickwar, Colin R.; Lieb, Jason D.] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA. [Lickwar, Colin R.; Lieb, Jason D.] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC 27599 USA. [Bower, Brian D.] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA. [Gorelick, Robert J.] SAIC Frederick Inc, AIDS & Canc Virus Program, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA. [Znosko, Brent M.] St Louis Univ, Dept Chem, St Louis, MO 63103 USA. RP Weeks, KM (reprint author), Univ N Carolina, Dept Chem, CB 3290, Chapel Hill, NC 27599 USA. EM weeks@unc.edu FU U.S. National Institutes of Health [GM064803, GM072518, GM031819]; National Cancer Institute [HHSN261200800001E]; SAIC-Frederick, Inc. FX We are indebted to D. Grawoig for a critical review of the manuscript and to D. Johnson and C. Hixson for assistance in preparing MuLV NC protein. This work was supported by the U.S. National Institutes of Health (GM064803 to K.M.W., GM072518 to J.D.L., and GM031819 to Jack Griffith and B.D.B) and by the National Cancer Institute (under contract HHSN261200800001E with SAIC-Frederick, Inc. to R.J.G.). Data sets of representative clustered kinetic data are provided in the supplementary materials. NR 22 TC 24 Z9 24 U1 0 U2 21 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD APR 12 PY 2013 VL 340 IS 6129 BP 190 EP 195 DI 10.1126/science.1230715 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 122TQ UT WOS:000317341400054 PM 23470731 ER PT J AU Lundberg, MS AF Lundberg, Martha S. TI Cardiovascular Tissue Engineering Research Support at the National Heart, Lung, and Blood Institute SO CIRCULATION RESEARCH LA English DT Editorial Material DE biomaterials; cardiovascular; regenerative medicine; stem cells; tissue engineering ID COLLAGEN; VESSEL; CELLS AB Tissue engineering aims at building 3-dimensional living substitutes that are equal to or better than the damaged tissue to be replaced. The development of such a tissue replacement requires a multidisciplinary approach and careful attention to the optimal cell source, the interactions of growth factors and extracellular milieu, and the scaffolding design. This article is a review of the tissue engineering programs of the National Heart, Lung, and Blood Institute, which support research efforts to translate novel approaches for the treatment of cardiovascular disease. Recent progress is discussed, which highlights some major questions relevant to cardiovascular tissue engineering. The National Heart, Lung, and Blood Institute has a strong interest in tissue engineering and will continue to foster the practical, clinical, and commercial development of research discoveries in this emerging field. (Circ Res. 2013; 112: 1097-1103.) C1 [Lundberg, Martha S.] NHLBI, Div Cardiovasc Sci DCVS, Bethesda, MD 20892 USA. RP Lundberg, MS (reprint author), NHLBI, DCVS, Rockledge 2,Rm 8210,6701 Rockledge Dr, Bethesda, MD 20892 USA. EM lundberm@nhlbi.nih.gov FU Intramural NIH HHS [Z99 HL999999] NR 14 TC 6 Z9 6 U1 0 U2 7 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7330 EI 1524-4571 J9 CIRC RES JI Circ.Res. PD APR 12 PY 2013 VL 112 IS 8 BP 1097 EP 1103 DI 10.1161/CIRCRESAHA.112.300638 PG 7 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA 124QW UT WOS:000317481700006 PM 23580772 ER PT J AU Dolja, VV Koonin, EV AF Dolja, Valerian V. Koonin, Eugene V. TI The closterovirus-derived gene expression and RNA interference vectors as tools for research and plant biotechnology SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE viral vector; closteroviruses; RNAi; Beet yellows virus; Citrus tristeza virus; Grapevine leafroll-associated virus-2 ID BEET-YELLOWS-VIRUS; CITRUS-TRISTEZA-VIRUS; CELL-TO-CELL; SUBGENOMIC MESSENGER-RNAS; LONG-DISTANCE TRANSPORT; CAPSID PROTEIN HOMOLOG; CIS-ACTING ELEMENTS; MINOR COAT PROTEIN; HSP70 HOMOLOG; FAMILY CLOSTEROVIRIDAE AB Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large closterovirus genomes offer genetic capacity and stability unrivaled by other plant viral vectors. These features provided immense opportunities for using closterovirus vectors for the functional genomics studies and pathogen control in economically valuable crops. This review briefly summarizes advances in closterovirus research during the last decade, explores the relationships between virus biology and vector design, and outlines the most promising directions for future application of closterovirus vectors. C1 [Dolja, Valerian V.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Dolja, Valerian V.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Koonin, Eugene V.] Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. RP Dolja, VV (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Cordley Hall 2082, Corvallis, OR 97331 USA. EM doljav@science.oregonstate.edu FU Vinoculate, Inc., Soledad, CA [2010-744]; USDA-NIFA [2009-04401]; BARD award [IS-4314-10C] FX The authors are grateful to Ellie Kurth and Valera Peremyslov for the images, The GLRaV-2 vector work in Valerian V. Dolia lab has been supported by contract 2010-744 with Vinoculate, Inc., Soledad, CA, subcontract with USDA-NIFA (award No, 2009-04401), and BARD award No IS-4314-10C, NR 93 TC 9 Z9 9 U1 1 U2 19 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 11 PY 2013 VL 4 AR 83 DI 10.3389/fmicb.2013.00083 PG 10 WC Microbiology SC Microbiology GA AA4EV UT WOS:000331049100001 PM 23596441 ER PT J AU Fowler, DH Mossoba, ME Steinberg, SM Halverson, DC Stroncek, D Khuu, HM Hakim, FT Castiello, L Sabatino, M Leitman, SF Mariotti, J Gea-Banacloche, JC Sportes, C Hardy, NM Hickstein, DD Pavletic, SZ Rowley, S Goy, A Donato, M Korngold, R Pecora, A Levine, BL June, CH Gress, RE Bishop, MR AF Fowler, Daniel H. Mossoba, Miriam E. Steinberg, Seth M. Halverson, David C. Stroncek, David Khuu, Hahn M. Hakim, Frances T. Castiello, Luciano Sabatino, Marianna Leitman, Susan F. Mariotti, Jacopo Gea-Banacloche, Juan C. Sportes, Claude Hardy, Nancy M. Hickstein, Dennis D. Pavletic, Steven Z. Rowley, Scott Goy, Andre Donato, Michele Korngold, Robert Pecora, Andrew Levine, Bruce L. June, Carl H. Gress, Ronald E. Bishop, Michael R. TI Phase 2 clinical trial of rapamycin-resistant donor CD4(+) Th2/Th1 (T-Rapa) cells after low-intensity allogeneic hematopoietic cell transplantation SO BLOOD LA English DT Article ID VERSUS-HOST-DISEASE; GRAFT-REJECTION; IN-VIVO; HEMATOLOGIC MALIGNANCIES; MAMMALIAN TARGET; ACUTE GVHD; TH2 CELLS; INHIBITION; LEUKEMIA; IMPACT AB In experimental models, ex vivo induced T-cell rapamycin resistance occurred independent of T helper 1 (Th1)/T helper 2 (Th2) differentiation and yielded allogeneic CD4(+) T cells of increased in vivo efficacy that facilitated engraftment and permitted graft-versus-tumor effects while minimizing graft-versus-host disease (GVHD). To translate these findings, we performed a phase 2 multicenter clinical trial of rapamycin-resistant donor CD4(+) Th2/Th1 (T-Rapa) cells after allogeneic-matched sibling donor hematopoietic cell transplantation (HCT) for therapy of refractory hematologic malignancy. T-Rapa cell products, which expressed a balanced Th2/Th1 phenotype, were administered as a preemptive donor lymphocyte infusion at day 14 post-HCT. After T-Rapa cell infusion, mixed donor/host chimerism rapidly converted, and there was preferential immune reconstitution with donor CD4(+) Th2 and Th1 cells relative to regulatory T cells and CD8(+) T cells. The cumulative incidence probability of acute GVHD was 20% and 40% at days 100 and 180 post-HCT, respectively. There was no transplant-related mortality. Eighteen of 40 patients (45%) remain in sustained complete remission(range of follow-up: 42-84 months). These results demonstrate the safety of this low-intensity transplant approach and the feasibility of subsequent randomized studies to compare T-Rapa cell-based therapy with standard transplantation regimens. This trial was registered at www.cancer.gov/clinicaltrials as #NCT 00077480. C1 [Fowler, Daniel H.; Mossoba, Miriam E.; Halverson, David C.; Hakim, Frances T.; Mariotti, Jacopo; Gea-Banacloche, Juan C.; Sportes, Claude; Hardy, Nancy M.; Hickstein, Dennis D.; Pavletic, Steven Z.; Gress, Ronald E.; Bishop, Michael R.] NCI, Expt Transplantat & Immunol Branch, Bethesda, MD 20892 USA. [Steinberg, Seth M.] NCI, Bethesda, MD 20892 USA. [Stroncek, David; Khuu, Hahn M.; Castiello, Luciano; Sabatino, Marianna; Leitman, Susan F.] NIH, Dept Transfus Med, Bethesda, MD 20892 USA. [Rowley, Scott; Goy, Andre; Donato, Michele; Korngold, Robert; Pecora, Andrew] Hackensack Univ, John Theurer Canc Ctr, Med Ctr, Hackensack, NJ USA. [Levine, Bruce L.; June, Carl H.] Univ Penn, Abramson Family Canc Res Ctr, Philadelphia, PA 19104 USA. RP Fowler, DH (reprint author), CRC, 10 Ctr Dr,Bldg 10,3-3330, Bethesda, MD USA. EM dhfowler@helix.nih.gov RI Castiello, Luciano/K-8616-2016 OI Castiello, Luciano/0000-0001-7146-3158 FU NCI Center for Cancer Research; NCI Center for Treatment and Evaluation Program FX This work was supported by the Intramural Research Program, NCI Center for Cancer Research; and was also supported by the NCI Center for Treatment and Evaluation Program, in particular Dr Howard Streicher, for provision of IL-4. NR 50 TC 17 Z9 17 U1 1 U2 7 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 11 PY 2013 VL 121 IS 15 BP 2864 EP 2874 DI 10.1182/blood-2012-08-446872 PG 11 WC Hematology SC Hematology GA 183OH UT WOS:000321825700011 PM 23426943 ER PT J AU Jessen, B Bode, SFN Ammann, S Chakravorty, S Davies, G Diestelhorst, J Frei-Jones, M Gahl, WA Gochuico, BR Griese, M Griffiths, G Janka, G Klein, C Kogl, T Kurnik, K Lehmberg, K Maul-Pavicic, A Mumford, AD Pace, D Parvaneh, N Rezaei, N de Saint Basile, G Schmitt-Graeff, A Schwarz, K Karasu, GT Zieger, B zur Stadt, U Aichele, P Ehl, S AF Jessen, Birthe Bode, Sebastian F. N. Ammann, Sandra Chakravorty, Subarna Davies, Graham Diestelhorst, Jana Frei-Jones, Melissa Gahl, William A. Gochuico, Bernadette R. Griese, Matthias Griffiths, Gillian Janka, Gritta Klein, Christoph Koegl, Tamara Kurnik, Karin Lehmberg, Kai Maul-Pavicic, Andrea Mumford, Andrew D. Pace, David Parvaneh, Nima Rezaei, Nima de Saint Basile, Genevieve Schmitt-Graeff, Annette Schwarz, Klaus Karasu, Gulsun T. Zieger, Barbara zur Stadt, Udo Aichele, Peter Ehl, Stephan TI The risk of hemophagocytic lymphohistiocytosis in Hermansky-Pudlak syndrome type 2 SO BLOOD LA English DT Article ID LYMPHOCYTIC CHORIOMENINGITIS VIRUS; GRISCELLI SYNDROME TYPE-2; CHEDIAK-HIGASHI-SYNDROME; AP-3 ADAPTER COMPLEX; CD8(+) T-CELLS; ANTIGEN PRESENTATION; NEUTROPHIL ELASTASE; BETA-3A SUBUNIT; DENDRITIC CELLS; LYTIC GRANULES AB Genetic disorders of lymphocyte cytotoxicity predispose patients to hemophagocytic lymphohistiocytosis (HLH). Reduced lymphocyte cytotoxicity has been demonstrated in Hermansky-Pudlak syndrome type 2 (HPS2), but only a single patient was reported who developed HLH. Because that patient also carried a potentially contributing heterozygous RAB27A mutation, the risk for HLH in HPS2 remains unclear. We analyzed susceptibility to HLH in the pearl mouse model of HPS2. After infection with lymphocytic choriomeningitis virus, pearl mice developed all key features of HLH, linked to impaired virus control caused by a moderate defect in CTL cytotoxicity in vivo. However, in contrast to perforin-deficient mice, the disease was transient, and all mice fully recovered and controlled the infection. An additional heterozygous Rab27a mutation did not aggravate the cytotoxicity defect or disease parameters. In the largest survey of 22HPS2 patients covering 234 patient years, we identified only 1 additional patient with HLH and 2 with incomplete transient HLH-like episodes, although cytotoxicity or degranulation was impaired in all 16 patients tested. HPS2 confers a risk for HLH that is lower than in Griscelli or Chediak-Higashi syndrome, probably because of a milder defect in cytotoxicity. Preemptive hematopoietic stem cell transplantation does not appear justified in HPS2. C1 [Jessen, Birthe; Bode, Sebastian F. N.; Ammann, Sandra; Maul-Pavicic, Andrea; Ehl, Stephan] Univ Med Ctr Freiburg, Ctr Chron Immunodeficiency, Freiburg, Germany. [Jessen, Birthe; Bode, Sebastian F. N.; Ammann, Sandra; Maul-Pavicic, Andrea; Ehl, Stephan] Univ Freiburg, Freiburg, Germany. [Chakravorty, Subarna] Imperial Coll London, Dept Med, London, England. [Davies, Graham] Great Ormond St Hosp Sick Children, Dept Immunol, London WC1N 3JH, England. [Diestelhorst, Jana; Griese, Matthias; Klein, Christoph; Kurnik, Karin] Univ Munich, Dept Pediat, Dr von Hauner Childrens Hosp, Munich, Germany. [Frei-Jones, Melissa] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA. [Gahl, William A.; Gochuico, Bernadette R.] NHGRI, Sect Human Biochem Genet, Med Genet Branch, NIH, Bethesda, MD 20892 USA. [Griffiths, Gillian] Addenbrookes Hosp, Cambridge Inst Med Res, Cambridge, England. [Janka, Gritta; Lehmberg, Kai] Univ Med Ctr Hamburg Eppendorf, Dept Pediat Hematol & Oncol, Hamburg, Germany. [Koegl, Tamara; Aichele, Peter] Univ Freiburg, Inst Med Microbiol & Hyg, Dept Immunol, Freiburg, Germany. [Mumford, Andrew D.] Univ Bristol, Bristol Heart Inst, Bristol, Avon, England. [Pace, David] Mater Dei Hosp, Dept Paediat, Msida, Malta. [Parvaneh, Nima] Univ Tehran Med Sci, Pediat Infect Dis Res Ctr, Tehran, Iran. [Rezaei, Nima] Univ Tehran Med Sci, Childrens Med Ctr, Res Ctr Immunodeficiencies, Tehran, Iran. [Rezaei, Nima] Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, Iran. [de Saint Basile, Genevieve] Univ Paris 05, Hop Necker Enfants Malad, INSERM, U768, Paris, France. [Schmitt-Graeff, Annette] Univ Med Ctr Freiburg, Inst Pathol, Freiburg, Germany. [Schwarz, Klaus] Univ Ulm, Inst Transfus Med, D-89069 Ulm, Germany. [Karasu, Gulsun T.] Akdeniz Univ, Fac Med, Div Hematol & Med Oncol, TR-07058 Antalya, Turkey. [Zieger, Barbara] Univ Med Ctr Freiburg, Ctr Pediat & Adolescent Med, Freiburg, Germany. [zur Stadt, Udo] Univ Med Ctr Hamburg Eppendorf, Ctr Diagnost, Hamburg, Germany. RP Ehl, S (reprint author), Ctr Chron Immunodeficiency, Breisacher Str 117, D-79106 Freiburg, Germany. EM stephan.ehl@uniklinik-freiburg.de RI Rezaei, Nima/B-4245-2008; OI Rezaei, Nima/0000-0002-3836-1827; Griffiths, Gillian/0000-0003-0434-5842; Ammann, Sandra/0000-0003-0385-1890 FU Bundesministerium fur Bildung und Forschung [BMBF 01 EO 0803]; Thyssen Stiftung; EU FP7 (CURE-HLH); National Human Genome Research Institute, National Institutes of Health FX This study was supported by the Bundesministerium fur Bildung und Forschung (BMBF 01 EO 0803), the Thyssen Stiftung, the EU FP7 (CURE-HLH), and in part by the Intramural Research Program of the National Human Genome Research Institute, National Institutes of Health. NR 50 TC 16 Z9 17 U1 0 U2 4 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 11 PY 2013 VL 121 IS 15 BP 2943 EP 2951 DI 10.1182/blood-2012-10-463166 PG 9 WC Hematology SC Hematology GA 183OH UT WOS:000321825700019 PM 23403622 ER PT J AU Morton, LM Dores, GM Tucker, MA Kim, CJ Onel, K Gilbert, ES Fraumeni, JF Curtis, RE AF Morton, Lindsay M. Dores, Graca M. Tucker, Margaret A. Kim, Clara J. Onel, Kenan Gilbert, Ethel S. Fraumeni, Joseph F., Jr. Curtis, Rochelle E. TI Evolving risk of therapy-related acute myeloid leukemia following cancer chemotherapy among adults in the United States, 1975-2008 SO BLOOD LA English DT Article ID NON-HODGKINS-LYMPHOMA; STEM-CELL TRANSPLANTATION; BREAST-CANCER; ESOPHAGEAL CANCER; MULTIPLE-MYELOMA; MYELODYSPLASTIC SYNDROMES; SECONDARY LEUKEMIA; OVARIAN-CANCER; LUNG-CANCER; CARCINOMA AB Therapy-related acute myeloid leukemia (tAML) is a rare but highly fatal complication of cytotoxic chemotherapy. Despite major changes in cancer treatment, data describing tAML risks over time are sparse. Among 426 068 adults initially treated with chemotherapy for first primary malignancy (9 US population-based cancer registries, 1975-2008), we identified 801 tAML cases, 4.70 times more than expected in the general population (P < .001). Over time, tAML risks increased after chemotherapy for non-Hodgkin lymphoma (n = 158; Poisson regression P-trend < .001), declined for ovarian cancer (n = 72; P-trend < .001), myeloma (n = 62; P-trend = .02), and possibly lung cancer (n = 65; P-trend = .18), and were significantly heterogeneous for breast cancer (n = 223; P-homogeneity = .005) and Hodgkin lymphoma (n = 58; P-homogeneity = .007). tAML risks varied significantly by age at first cancer and latency and were non-significantly heightened with radiotherapy for lung, breast, and ovarian cancers. We identified newly emerging elevated tAML risks in patients treated with chemotherapy since 2000 for esophageal, cervical, prostate, and possibly anal cancers; and since the 1990s for bone/joint and endometrial cancers. Using long-term, population-based data, we observed significant variation in tAML risk with time, consistent with changing treatment practices and differential leukemogenicity of specific therapies. tAML risks should be weighed against the benefits of chemotherapy, particularly for new agents and new indications for standard agents. C1 [Morton, Lindsay M.; Dores, Graca M.; Tucker, Margaret A.; Kim, Clara J.; Gilbert, Ethel S.; Fraumeni, Joseph F., Jr.; Curtis, Rochelle E.] NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Dores, Graca M.] Dept Vet Affairs Med Ctr, Oklahoma City, OK USA. [Onel, Kenan] Univ Chicago, Dept Pediat, Chicago, IL 60637 USA. RP Morton, LM (reprint author), NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, 6120 Execut Blvd EPS 7040 MSC 7238, Rockville, MD 20892 USA. EM mortonli@mail.nih.gov RI Tucker, Margaret/B-4297-2015; Morton, Lindsay/B-5234-2015 OI Morton, Lindsay/0000-0001-9767-2310 FU National Cancer Institute, National Institutes of Health, Department of Health and Human Services FX This study was supported by the Intramural Program of the National Cancer Institute, National Institutes of Health, Department of Health and Human Services. NR 49 TC 55 Z9 57 U1 0 U2 1 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 11 PY 2013 VL 121 IS 15 BP 2996 EP 3004 DI 10.1182/blood-2012-08-448068 PG 9 WC Hematology SC Hematology GA 183OH UT WOS:000321825700024 PM 23412096 ER PT J AU Hosseini, P Ovcharenko, I Matthews, BF AF Hosseini, Parsa Ovcharenko, Ivan Matthews, Benjamin F. TI Using an ensemble of statistical metrics to quantify large sets of plant transcription factor binding sites SO PLANT METHODS LA English DT Article ID CIS-REGULATORY ELEMENTS; ARABIDOPSIS; ASSOCIATION; DEFENSE; PROMOTERS; NETWORKS; DATABASE; STRESS; MOTIFS; LIGHT AB Background: From initial seed germination through reproduction, plants continuously reprogram their transcriptional repertoire to facilitate growth and development. This dynamic is mediated by a diverse but inextricably-linked catalog of regulatory proteins called transcription factors (TFs). Statistically quantifying TF binding site (TFBS) abundance in promoters of differentially expressed genes can be used to identify binding site patterns in promoters that are closely related to stress-response. Output from today's transcriptomic assays necessitates statistically-oriented software to handle large promoter-sequence sets in a computationally tractable fashion. Results: We present Marina, an open-source software for identifying over-represented TFBSs from amongst large sets of promoter sequences, using an ensemble of 7 statistical metrics and binding-site profiles. Through software comparison, we show that Marina can identify considerably more over-represented plant TFBSs compared to a popular software alternative. Conclusions: Marina was used to identify over-represented TFBSs in a two time-point RNA-Seq study exploring the transcriptomic interplay between soybean (Glycine max) and soybean rust (Phakopsora pachyrhizi). Marina identified numerous abundant TFBSs recognized by transcription factors that are associated with defense-response such as WRKY, HY5 and MYB2. Comparing results from Marina to that of a popular software alternative suggests that regardless of the number of promoter-sequences, Marina is able to identify significantly more over-represented TFBSs. C1 [Hosseini, Parsa] George Mason Univ, Dept Bioinformat & Computat Biol, Manassas, VA USA. [Hosseini, Parsa; Ovcharenko, Ivan] NIH, Computat Biol Branch, Natl Ctr Biotechnol Informat, Bethesda, MD 20892 USA. [Hosseini, Parsa; Matthews, Benjamin F.] USDA, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Hosseini, P (reprint author), George Mason Univ, Dept Bioinformat & Computat Biol, Manassas, VA USA. EM parsa.hosseini@nih.gov FU United States Department of Agriculture - Soybean Genomics and Improvement Laboratory (USDA - SGIL); Intramural Research Program of the National Institutes of Health, National Library of Medicine FX We wish to thank the United States Department of Agriculture - Soybean Genomics and Improvement Laboratory (USDA - SGIL) for research funding and support. We also wish to thank Margaret MacDonald, Eric Brewer, Sara Kabir and Leila Taher for their comments on Marina functionality. Our appreciations also go out to Bret Cooper for advice on RNA-Seq quality analysis and correction. This research was supported in part by the Intramural Research Program of the National Institutes of Health, National Library of Medicine. NR 57 TC 1 Z9 1 U1 0 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-4811 J9 PLANT METHODS JI Plant Methods PD APR 11 PY 2013 VL 9 AR 12 DI 10.1186/1746-4811-9-12 PG 11 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 138EZ UT WOS:000318492900001 PM 23578135 ER PT J AU Bennett, TM Maraini, G Jin, CF Sun, W Hejtmancik, JF Shiels, A AF Bennett, Thomas M. Maraini, Giovanni Jin, Chongfei Sun, Wenmin Hejtmancik, J. Fielding Shiels, Alan TI Noncoding variation of the gene for ferritin light chain in hereditary and age-related cataract SO MOLECULAR VISION LA English DT Article ID IRON-RESPONSIVE ELEMENT; CONGENITAL CATARACT; MISSENSE MUTATION; GLYCOSYLATED FERRITIN; BILATERAL CATARACT; MOLECULAR-BASIS; POINT MUTATION; MESSENGER-RNA; HYPERFERRITINEMIA; LINKAGE AB Purpose: Cataract is a clinically and genetically heterogeneous disorder of the ocular lens and an important cause of visual impairment. The aim of this study was to map and identify the gene underlying autosomal dominant cataract segregating in a four-generation family, determine the lens expression profile of the identified gene, and test for its association with age-related cataract in a case-control cohort. Methods: Genomic DNA was prepared from blood leukocytes, and genotyping was performed by means of single-nucleotide polymorphism markers and microsatellite markers. Linkage analyses were performed using the GeneHunter and MLINK programs, and mutation detection was achieved by dideoxy cycle sequencing. Lens expression studies were performed using reverse-transcription polymerase chain reaction (RT-PCR) and in situ hybridization. Results: Genome-wide linkage analysis with single nucleotide polymorphism markers in the family identified a likely disease-haplotype interval on chromosome 19q (rs888861-[similar to 17Mb]-rs8111640) that encompassed the microsatellite marker D19S879 (logarithm of the odds score [Z]= 2.03, recombination distance [theta]= 0). Mutation profiling of positional-candidate genes detected a heterozygous, noncoding G-to-T transversion (c.-168G> T) located in the iron response element (IRE) of the gene coding for ferritin light chain (FTL) that cosegregated with cataract in the family. Serum ferritin levels were found to be abnormally elevated (similar to fourfold), without evidence of iron overload, in an affected family member; this was consistent with a diagnosis of hereditary hyperferritinemia-cataract syndrome. No sequence variations located within the IRE were detected in a cohort of 197 cases with age-related cataract and 102 controls with clear lenses. Expression studies of human FTL, and its mouse counterpart FTL1, in the lens detected RT-PCR amplicons containing full-length protein-coding regions, and strong in situ localization of FTL1 transcripts to the lens equatorial epithelium and peripheral cortex. Conclusions: The data are consistent with robust transcription of FTL in the lens, and suggest that whereas variations clustered in the IRE of the FTL gene are directly associated with hereditary hyperferritinemia-cataract syndrome, such IRE variations are unlikely to play a significant role in the genetic etiology of age-related cataract. C1 [Bennett, Thomas M.; Shiels, Alan] Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USA. [Maraini, Giovanni] Univ Parma, Dept Ophthalmol, I-43100 Parma, Italy. [Jin, Chongfei; Sun, Wenmin; Hejtmancik, J. Fielding] NEI, Ophthalm Genet & Visual Funct Branch, NIH, Bethesda, MD 20892 USA. RP Shiels, A (reprint author), Washington Univ, Sch Med, Box 8096,660 S Euclid Ave, St Louis, MO 63110 USA. EM shiels@vision.wustl.edu FU NIH/NEI [RO1EY012284, P30EY02687]; Research to Prevent Blindness (RPB); FIL FX We thank the family for participating in this study, and Belinda McMahan for expert histological assistance. This work was supported by NIH/NEI grants RO1EY012284 (A.S.) and P30EY02687 (Core Grant for Vision Research), an unrestricted grant to the Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness (RPB), and by FIL 2002-2003 (G.M.). NR 43 TC 3 Z9 3 U1 0 U2 4 PU MOLECULAR VISION PI ATLANTA PA C/O JEFF BOATRIGHT, LAB B, 5500 EMORY EYE CENTER, 1327 CLIFTON RD, N E, ATLANTA, GA 30322 USA SN 1090-0535 J9 MOL VIS JI Mol. Vis. PD APR 11 PY 2013 VL 19 BP 835 EP 844 PG 10 WC Biochemistry & Molecular Biology; Ophthalmology SC Biochemistry & Molecular Biology; Ophthalmology GA 126II UT WOS:000317607100001 PM 23592921 ER PT J AU Dunleavy, K Pittaluga, S Maeda, LS Advani, R Chen, CC Hessler, J Steinberg, SM Grant, C Wright, G Varma, G Staudt, LM Jaffe, ES Wilson, WH AF Dunleavy, Kieron Pittaluga, Stefania Maeda, Lauren S. Advani, Ranjana Chen, Clara C. Hessler, Julie Steinberg, Seth M. Grant, Cliona Wright, George Varma, Gaurav Staudt, Louis M. Jaffe, Elaine S. Wilson, Wyndham H. TI Dose-Adjusted EPOCH-Rituximab Therapy in Primary Mediastinal B-Cell Lymphoma SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID CLASSICAL HODGKIN LYMPHOMA; GERMINAL-CENTER; CHEMOTHERAPY; DOXORUBICIN; AMPLIFICATION; RADIOTHERAPY; EXPRESSION; MANAGEMENT; SCLEROSIS; EFFICACY AB BACKGROUND Primary mediastinal B-cell lymphoma is a distinct subtype of diffuse large-B-cell lymphoma that is closely related to nodular sclerosing Hodgkin's lymphoma. Patients are usually young and present with large mediastinal masses. There is no standard treatment, but the inadequacy of immunochemotherapy alone has resulted in routine consolidation with mediastinal radiotherapy, which has potentially serious late effects. We aimed to develop a strategy that improves the rate of cure and obviates the need for radiotherapy. METHODS We conducted a single-group, phase 2, prospective study of infusional dose-adjusted etoposide, doxorubicin, and cyclophosphamide with vincristine, prednisone, and rituximab (DA-EPOCH-R) and filgrastim without radiotherapy in 51 patients with untreated primary mediastinal B-cell lymphoma. We used results from a retrospective study of DA-EPOCH-R from another center to independently verify the outcomes. RESULTS The patients had a median age of 30 years (range, 19 to 52) and a median tumor diameter of 11 cm; 59% were women. During a median of 5 years of follow-up, the event-free survival rate was 93%, and the overall survival rate was 97%. Among the 16 patients who were involved in the retrospective analysis at another center, over a median of 3 years of follow-up, the event-free survival rate was 100%, and no patients received radiotherapy. No late morbidity or cardiac toxic effects were found in any patients. After follow-up ranging from 10 months to 14 years, all but 2 of the 51 patients (4%) who received DA-EPOCH-R alone were in complete remission. The 2 remaining patients received radiotherapy and were disease-free at follow-up. CONCLUSIONS Therapy with DA-EPOCH-R obviated the need for radiotherapy in patients with primary mediastinal B-cell lymphoma. (Funded by the National Cancer Institute; ClinicalTrials.gov number, NCT00001337.) C1 [Dunleavy, Kieron; Pittaluga, Stefania; Hessler, Julie; Steinberg, Seth M.; Grant, Cliona; Staudt, Louis M.; Jaffe, Elaine S.; Wilson, Wyndham H.] NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Chen, Clara C.] NCI, Ctr Clin, NIH, Bethesda, MD 20892 USA. [Wright, George] NCI, Biometr Res Branch, Div Canc Treatment & Diag, NIH, Bethesda, MD 20892 USA. [Maeda, Lauren S.; Advani, Ranjana; Varma, Gaurav] Stanford Univ, Sch Med, Div Oncol, Stanford, CA 94305 USA. RP Wilson, WH (reprint author), NCI, Metab Branch, Bldg 10,Rm 4N-115,9000 Rockville Pike, Bethesda, MD 20892 USA. EM wilsonw@mail.nih.gov OI Jaffe, Elaine/0000-0003-4632-0301; Varma, Gaurav/0000-0002-1903-9170 FU National Cancer Institute FX Funded by the National Cancer Institute; Supported by the National Cancer Institute. NR 29 TC 125 Z9 129 U1 1 U2 9 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 11 PY 2013 VL 368 IS 15 BP 1408 EP 1416 DI 10.1056/NEJMoa1214561 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA 122QT UT WOS:000317333600008 PM 23574119 ER PT J AU Sharma, A Sen, JM AF Sharma, A. Sen, J. M. TI Molecular basis for the tissue specificity of beta-catenin oncogenesis SO ONCOGENE LA English DT Article DE beta-catenin; hypocellular thymus; p53; apoptosis; intestinal adenoma; c-Myc ID ACUTE LYMPHOBLASTIC-LEUKEMIA; T-CELL DEVELOPMENT; MULTIPLE INTESTINAL NEOPLASIA; IN-VIVO; MEDIATED TRANSCRIPTION; THYMOCYTE DEVELOPMENT; APC(MIN/+) MOUSE; GENE-EXPRESSION; APC DEFICIENCY; MYC DELETION AB Wnt-beta-catenin-T-cell factor signaling is causally linked to c-myc-dependent tumorigenesis in mouse and human colon epithelial cells. By contrast, beta-catenin is not similarly associated with oncogenic transformation of other tissues, including T cells. The molecular basis for tissue specificity of beta-catenin-dependent oncogenesis is unknown. Here, we demonstrate that adenomatous polyposis coli mutant APC(Min/-) mice, which have increased expression of beta-catenin in all tissues, develop severe intestinal neoplasia, but fail to develop thymic lymphoma. Whereas beta-catenin-dependent signals elicit a proliferative response from intestinal cells, thymocytes experience oncogene-induced senescence (OIS), growth arrest and apoptosis. We demonstrate that the differential cellular response of thymocytes and intestinal epithelial cells is a direct consequence of the gene expression elicited by beta-catenin expression in each tissue. We find that whereas intestinal cells induce genes that promote proliferation thymocytes induce expression of genes associated with OIS, growth arrest and p53-dependent apoptosis. We correlate gene expression pattern with the role beta-catenin plays in the development of each tissue and suggest that susceptibility of transformation by beta-catenin is intimately related to its function during development. We propose that when oncogenes are used as signaling molecules, safety nets in the form of OIS, growth arrest and apoptosis prevent accidental transformation. Oncogene (2013) 32, 1901-1909; doi:10.1038/onc.2012.215; published online 11 June 2012 C1 [Sharma, A.; Sen, J. M.] NIA, Lymphocyte Dev Unit, Lab Mol Biol & Immunol, NIH, Baltimore, MD 21224 USA. RP Sen, JM (reprint author), NIA, Lymphocyte Dev Unit, Lab Mol Biol & Immunol, NIH, Baltimore, MD 21224 USA. EM Jyoti-Sen@NIH.GOV RI Sharma, Archna/R-9377-2016 OI Sharma, Archna/0000-0003-4745-0220 FU National Institute on Aging; National Institutes of Health FX We thank Q Yu for doing IP BrdU injections; R Wersto and the FACS facility team for cell sorting; the animal facility of National Institute on Aging for maintaining animals; S Luo and team for genotyping. This research was supported by Intramural Research Program of the National Institute on Aging and National Institutes of Health. NR 54 TC 7 Z9 8 U1 0 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD APR 11 PY 2013 VL 32 IS 15 BP 1901 EP 1909 DI 10.1038/onc.2012.215 PG 9 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 126GB UT WOS:000317599900004 PM 22689057 ER PT J AU Stuelten, CH Cervoni-Curet, FN Busch, JI Sutton, E Webster, JD Kavalukas, SL Wakefield, LM Barbul, A Niederhuber, JE AF Stuelten, Christina H. Cervoni-Curet, Frances N. Busch, Johanna I. Sutton, Emily Webster, Joshua D. Kavalukas, Sandra L. Wakefield, Lalage M. Barbul, Adrian Niederhuber, John E. TI SDF-1 alpha Mediates Wound-Promoted Tumor Growth in a Syngeneic Orthotopic Mouse Model of Breast Cancer SO PLOS ONE LA English DT Article ID RECEPTOR CXCR4; ANTAGONIST; MICROENVIRONMENT; MOBILIZATION; AMD3100; REPAIR; CELLS AB Increased growth of residual tumors in the proximity of acute surgical wounds has been reported; however, the mechanisms of wound-promoted tumor growth remain unknown. Here, we used a syngeneic, orthotopic mouse model of breast cancer to study mechanisms of wound-promoted tumor growth. Our results demonstrate that exposure of metastatic mouse breast cancer cells (4T1) to SDF-1 alpha, which is increased in wound fluid, results in increased tumor growth. Both, wounding and exposure of 4T1 cells to SDF-1 alpha not only increased tumor growth, but also tumor cell proliferation rate and stromal collagen deposition. Conversely, systemic inhibition of SDF-1 alpha signaling with the small molecule AMD 3100 abolished the effect of wounding, and decreased cell proliferation, collagen deposition, and neoangiogenesis to the levels observed in control animals. Furthermore, using different mouse strains we could demonstrate that the effect of wounding on tumor growth and SDF-1 alpha levels is host dependent and varies between mouse strains. Our results show that wound-promoted tumor growth is mediated by elevated SDF-1 alpha levels and indicate that the effect of acute wounds on tumor growth depends on the predetermined wound response of the host background and its predetermined wound response. C1 [Stuelten, Christina H.; Cervoni-Curet, Frances N.; Busch, Johanna I.; Sutton, Emily; Niederhuber, John E.] NCI, Cell & Canc Biol Branch, Bethesda, MD 20892 USA. [Stuelten, Christina H.] NCI, Lab Cellular & Mol Biol, Bethesda, MD 20892 USA. [Webster, Joshua D.; Wakefield, Lalage M.] NCI, Lab Canc Biol & Genet, Bethesda, MD 20892 USA. [Kavalukas, Sandra L.; Barbul, Adrian] Sinai Hosp, Dept Surg, Baltimore, MD 21215 USA. [Barbul, Adrian] Johns Hopkins Med Sch, Dept Surg, Baltimore, MD USA. RP Stuelten, CH (reprint author), NCI, Cell & Canc Biol Branch, Bethesda, MD 20892 USA. EM chrisstu@mail.nih.gov FU Intramural Research Program of the Center for Cancer Research, NCI, National Institutes of Health FX This research was supported by the Intramural Research Program of the Center for Cancer Research, NCI, National Institutes of Health (http://www.cancer.gov/researchandfunding/intramural). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 22 TC 3 Z9 3 U1 1 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 11 PY 2013 VL 8 IS 4 AR e60919 DI 10.1371/journal.pone.0060919 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 123JA UT WOS:000317383200028 PM 23593347 ER PT J AU Nussinov, R Tsai, CJ AF Nussinov, Ruth Tsai, Chung-Jung TI Allostery in Disease and in Drug Discovery SO CELL LA English DT Review ID PROTEIN-COUPLED RECEPTOR; BCR-ABL; CONFORMATIONAL ENSEMBLES; ENERGY LANDSCAPES; BINDING CASCADES; DISTANT SITES; MAP KINASE; INHIBITORS; ACTIVATION; DYNAMICS AB Allostery is largely associated with conformational and functional transitions in individual proteins. This concept can be extended to consider the impact of conformational perturbations on cellular function and disease states. Here, we clarify the concept of allostery and how it controls physiological activities. We focus on the challenging questions of how allostery can both cause disease and contribute to development of new therapeutics. We aim to increase the awareness of the linkage between disease symptoms on the cellular level and specific aberrant allosteric actions on the molecular level and to emphasize the potential of allosteric drugs in innovative therapies. C1 [Nussinov, Ruth; Tsai, Chung-Jung] NCI, Ctr Canc Res, Nanobiol Program, Frederick Natl Lab Canc Res,SAIC Frederick Inc, Frederick, MD 21702 USA. [Nussinov, Ruth] Tel Aviv Univ, Sackler Inst Mol Med, Sackler Sch Med, Dept Human Genet & Mol Med, IL-69978 Tel Aviv, Israel. RP Nussinov, R (reprint author), NCI, Ctr Canc Res, Nanobiol Program, Frederick Natl Lab Canc Res,SAIC Frederick Inc, Frederick, MD 21702 USA. EM nussinor@helix.nih.gov RI Marion-Poll, Frederic/D-8882-2011 OI Marion-Poll, Frederic/0000-0001-6824-0180 FU NCI, NIH [HHSN261200800001E]; Intramural Research Program of the NIH, NCI, CCR FX This project has been funded in whole or in part with Federal funds from the NCI, NIH, under contract number HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of DHHS, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. This research was supported in part by the Intramural Research Program of the NIH, NCI, CCR. NR 96 TC 151 Z9 151 U1 4 U2 107 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 J9 CELL JI Cell PD APR 11 PY 2013 VL 153 IS 2 BP 293 EP 305 DI 10.1016/j.cell.2013.03.034 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 122WR UT WOS:000317349700010 PM 23582321 ER PT J AU Subramanian, N Natarajan, K Clatworthy, MR Wang, Z Germain, RN AF Subramanian, Naeha Natarajan, Kannan Clatworthy, Menna R. Wang, Ze Germain, Ronald N. TI The Adaptor MAVS Promotes NLRP3 Mitochondrial Localization and Inflammasome Activation SO CELL LA English DT Article ID ANTIVIRAL SIGNALING PROTEIN; CASPASE-1 ACTIVATION; REPERFUSION INJURY; OXIDATIVE STRESS; IMMUNE-RESPONSE; CELLS; FORMS; ATP; ASC; EXPRESSION AB NLRP3 is a key component of the macromolecular signaling complex called the inflammasome that promotes caspase 1-dependent production of IL-1 beta. The adaptor ASC is necessary for NLRP3-dependent inflammasome function, but it is not known whether ASC is a sufficient partner and whether inflammasome formation occurs in the cytosol or in association with mitochondria is controversial. Here, we show that the mitochondria-associated adaptor molecule, MAVS, is required for optimal NLRP3 inflammasome activity. MAVS mediates recruitment of NLRP3 to mitochondria, promoting production of IL-1b and the pathophysiologic activity of the NLRP3 inflammasome in vivo. Our data support a more complex model of NLRP3 inflammasome activation than previously appreciated, with at least two adapters required for maximal function. Because MAVS is a mitochondria-associated molecule previously considered to be uniquely involved in type 1 interferon production, these findings also reveal unexpected polygamous involvement of PYD/CARD-domain-containing adapters in innate immune signaling events. C1 [Subramanian, Naeha; Clatworthy, Menna R.; Wang, Ze; Germain, Ronald N.] NIAID, Lymphocyte Biol Sect, Lab Syst Biol, NIH, Bethesda, MD 20892 USA. [Natarajan, Kannan] NIAID, Mol Biol Sect, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Clatworthy, Menna R.] Univ Cambridge, Addenbrookes Hosp, Sch Clin Med, Cambridge Inst Med Res, Cambridge CB2 0XY, England. RP Subramanian, N (reprint author), NIAID, Lymphocyte Biol Sect, Lab Syst Biol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM subramaniann@niaid.nih.gov; rgermain@nih.gov FU Intramural Research Program, NIAID, NIH; Wellcome Trust [WT081020] FX This work was supported by the Intramural Research Program, NIAID, NIH, and a Wellcome Trust Intermediate Fellowship (WT081020) to M. R. C. We are grateful to Drs. Daniel Kastner, Michael Lenardo, and Iain Fraser for critical reading of the manuscript. We thank the Biological Imaging Section, NIAID, NIH for excellent imaging support; Dr. Vishva Dixit and Dr. Zhijian Chen for kindly providing mouse strains; Dr. Eicke Latz for generously providing HEK-293-ASC-YFP cells; Dr. Iain Fraser for providing lentiviral constructs; and Drs. Wolfgang Kastenmuller, Marlene Brandes, and Jae Jin Chae for helpful discussions. The authors declare that they have no competing financial interests. NR 41 TC 134 Z9 137 U1 3 U2 55 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 J9 CELL JI Cell PD APR 11 PY 2013 VL 153 IS 2 BP 348 EP 361 DI 10.1016/j.cell.2013.02.054 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 122WR UT WOS:000317349700014 PM 23582325 ER PT J AU Babineau, BA Yang, M Berman, RF Crawley, JN AF Babineau, Brooke A. Yang, Mu Berman, Robert F. Crawley, Jacqueline N. TI Low home cage social behaviors in BTBR T plus tf/J mice during juvenile development SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Inbred strain; Home cage observation; Social interaction ID INBRED MOUSE STRAINS; ULTRASONIC VOCALIZATIONS; UNUSUAL REPERTOIRE; SCENT-MARKING; T+TF/J MICE; AUTISM; MODEL; C57BL/6J; SOCIABILITY; PHENOTYPES AB BTBR T+tf/J (BTBR) is a genetically homogenous inbred strain of mice that displays abnormal social behaviors, deficits in vocalizations, and high levels of repetitive behaviors, relevant to the three diagnostic symptoms of autism spectrum disorder, leading to the use of this strain as a mouse model of autism. Comprehensive observations of BTBR social behaviors within the home cage during early stages of development have not been conducted. Here we evaluate the home cage behaviors of BTBR in two laboratory environments (NIMH, Bethesda, Maryland vs. UC Davis, Davis, California), starting from the day of weaning and continuing into adulthood. Extensive ethogram parameters were scored for BTBR in home cages that contained four BTBR conspecifics, versus home cages that contained four C57BL/6 J (B6) conspecifics. BTBR were considerably less interactive than B6 in the home cage at both sites, as measured during the early dark stage of their circadian cycle. A novel home cage behavioral measure, frequency of long interactions, was found to be more frequent and of longer duration in B6 versus BTBR home cages across experimental sites. Significant strain differences in the occurrence of investigative and affiliative behaviors were also seen, however these findings were not fully consistent across the two testing sites. At the end of the 30-day home cage observation period, each seven-week old subject mouse was tested in the three-chambered social approach task. BTBR displayed lack of sociability and B6 displayed significant sociability, consistent with previous reports. Our findings reveal that BTBR engaged in lower levels of some components of spontaneous conspecific social interactions in the home cage environment throughout juvenile development, consistent with their deficits in juvenile and adult sociability as measured in specialized social tasks. (C) 2013 Elsevier Inc. All rights reserved. C1 [Babineau, Brooke A.; Yang, Mu; Crawley, Jacqueline N.] NIMH, Intramural Res Program, Lab Behav Neurosci, Bethesda, MD 20892 USA. [Berman, Robert F.] Univ Calif Davis, Dept Neurol Surg, Davis, CA 95616 USA. RP Babineau, BA (reprint author), Univ Calif San Francisco, Sch Med, 513 Parnassus Ave HSE-901, San Francisco, CA 94143 USA. EM Brooke.Babineau@ucsf.edu FU National Institute of Mental Health FX Supported by the National Institute of Mental Health Intramural Research Program. NR 31 TC 6 Z9 6 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD APR 10 PY 2013 VL 114 BP 49 EP 54 DI 10.1016/j.physbeh.2013.03.006 PG 6 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA 156VZ UT WOS:000319853300008 PM 23510981 ER PT J AU Liu, YC Guo, JT Hu, GQ Zhu, HQ AF Liu, Yongchu Guo, Jiangtao Hu, Gangqing Zhu, Huaiqiu TI Gene prediction in metagenomic fragments based on the SVM algorithm SO BMC BIOINFORMATICS LA English DT Article ID TRANSLATION INITIATION SITE; SUPPORT-VECTOR-MACHINE; MICROBIAL GENOMES; PROKARYOTIC GENOMES; SHOTGUN SEQUENCES; BACTERIAL GENOMES; IDENTIFICATION; ANNOTATION; GLIMMER; CLASSIFICATION AB Background: Metagenomic sequencing is becoming a powerful technology for exploring micro-ogranisms from various environments, such as human body, without isolation and cultivation. Accurately identifying genes from metagenomic fragments is one of the most fundamental issues. Results: In this article, we present a novel gene prediction method named MetaGUN for metagenomic fragments based on a machine learning approach of SVM. It implements in a three-stage strategy to predict genes. Firstly, it classifies input fragments into phylogenetic groups by a k-mer based sequence binning method. Then, protein-coding sequences are identified for each group independently with SVM classifiers that integrate entropy density profiles (EDP) of codon usage, translation initiation site (TIS) scores and open reading frame (ORF) length as input patterns. Finally, the TISs are adjusted by employing a modified version of MetaTISA. To identify protein-coding sequences, MetaGun builds the universal module and the novel module. The former is based on a set of representative species, while the latter is designed to find potential functionary DNA sequences with conserved domains. Conclusions: Comparisons on artificial shotgun fragments with multiple current metagenomic gene finders show that MetaGUN predicts better results on both 3' and 5' ends of genes with fragments of various lengths. Especially, it makes the most reliable predictions among these methods. As an application, MetaGUN was used to predict genes for two samples of human gut microbiome. It identifies thousands of additional genes with significant evidences. Further analysis indicates that MetaGUN tends to predict more potential novel genes than other current metagenomic gene finders. C1 [Liu, Yongchu; Guo, Jiangtao; Hu, Gangqing; Zhu, Huaiqiu] Peking Univ, State Key Lab Turbulence & Complex Syst, Beijing 100871, Peoples R China. [Liu, Yongchu; Guo, Jiangtao; Hu, Gangqing; Zhu, Huaiqiu] Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China. [Liu, Yongchu; Guo, Jiangtao; Hu, Gangqing; Zhu, Huaiqiu] Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China. [Zhu, Huaiqiu] Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China. [Hu, Gangqing] NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. RP Zhu, HQ (reprint author), Peking Univ, State Key Lab Turbulence & Complex Syst, Beijing 100871, Peoples R China. EM hqzhu@pku.edu.cn RI HU, GANGQING/K-5849-2012; Zhu, Huaiqiu/C-3617-2012 OI Zhu, Huaiqiu/0000-0002-6376-218X FU National Key Technology Research and Design Program of China [2012BAI06B02]; National Natural Science Foundation of China [30970667, 11021463, 61131003, 91231119]; National Basic Research Program of China [2011CB707500]; Excellent Doctoral Dissertation Supervisor Funding of Beijing [YB20101000102] FX Publication of this article was supported by the National Key Technology Research and Design Program of China (2012BAI06B02), National Natural Science Foundation of China (30970667, 11021463, 61131003 and 91231119), National Basic Research Program of China (2011CB707500), and Excellent Doctoral Dissertation Supervisor Funding of Beijing (YB20101000102). NR 49 TC 12 Z9 13 U1 2 U2 29 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2105 J9 BMC BIOINFORMATICS JI BMC Bioinformatics PD APR 10 PY 2013 VL 14 SU 5 AR S12 DI 10.1186/1471-2105-14-S5-S12 PG 12 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Mathematical & Computational Biology GA 142SB UT WOS:000318816300012 PM 23735199 ER PT J AU Hook, EW Shafer, W Deal, C Kirkcaldy, RD Iskander, J AF Hook, Edward W., III Shafer, William Deal, Carolyn Kirkcaldy, Robert D. Iskander, John TI CDC Grand Rounds: The Growing Threat of Multidrug-Resistant Gonorrhea (Reprinted from MMWR, vol 62, pg 103-106, 2013) SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Reprint ID SEXUALLY-TRANSMITTED-DISEASES; NEISSERIA-GONORRHOEAE; GONOCOCCAL INFECTIONS; TREATMENT-GUIDELINES; NO LONGER; CEFTRIAXONE; UPDATE C1 [Hook, Edward W., III] Univ Alabama Birmingham, Birmingham, AL USA. [Shafer, William] Emory Univ, Atlanta, GA 30322 USA. [Deal, Carolyn] NIAID, NIH, Bethesda, MD 20892 USA. [Kirkcaldy, Robert D.] CDC, Off Associate Director Sci, Atlanta, GA 30333 USA. EM rkirkcaldy@cdc.gov NR 15 TC 1 Z9 1 U1 0 U2 5 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 10 PY 2013 VL 309 IS 14 BP 1453 EP 1455 DI 10.1001/jama.2013.2131 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA 121VD UT WOS:000317271700007 ER PT J AU Moye, LA Simari, RD Skarlatos, SI AF Moye, Lemuel A. Simari, Robert D. Skarlatos, Sonia I. CA Cardiovasc Cell Therapy Res Networ TI Bone Marrow-Derived Cell Therapy After Myocardial Infarction SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter ID LEFT-VENTRICULAR FUNCTION; DELIVERY; TRIAL C1 [Moye, Lemuel A.] Univ Texas Houston, Sch Publ Hlth, Houston, TX 77030 USA. [Simari, Robert D.] Mayo Clin, Rochester, MN USA. [Skarlatos, Sonia I.] NHLBI, Bethesda, MD 20892 USA. RP Moye, LA (reprint author), Univ Texas Houston, Sch Publ Hlth, Houston, TX 77030 USA. EM lemmoye@msn.com FU NHLBI NIH HHS [UM1 HL087318, UM1 HL087394] NR 5 TC 3 Z9 3 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 10 PY 2013 VL 309 IS 14 BP 1459 EP 1459 DI 10.1001/jama.2013.2601 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA 121VD UT WOS:000317271700013 PM 23571574 ER PT J AU Guttmacher, AE Spong, CY Willinger, M AF Guttmacher, Alan E. Spong, Catherine Y. Willinger, Marian TI Long QT Syndrome Susceptibility Mutations and Pregnancy Loss Another Piece of a Still Unfinished Puzzle? SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material ID DEATH; FETAL C1 [Guttmacher, Alan E.; Spong, Catherine Y.; Willinger, Marian] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD USA. RP Guttmacher, AE (reprint author), NHGRI, NIH, 31 Ctr Dr,Room 2A03, Bethesda, MD 20892 USA. EM guttmach@mail.nih.gov NR 12 TC 3 Z9 3 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 10 PY 2013 VL 309 IS 14 BP 1525 EP 1526 DI 10.1001/jama.2013.3373 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 121VD UT WOS:000317271700031 PM 23571592 ER PT J AU Chandana, SR Kotecha, R Al-Janadi, A Chang, HT Conley, BA AF Chandana, Sreenivasa R. Kotecha, Rupesh Al-Janadi, Anas Chang, Howard T. Conley, Barbara A. TI Rare Case of Hairy Cell Leukemia With Brain Parenchymal Involvement: A Diagnostic Dilemma SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID CLINICAL-MANIFESTATIONS; MENINGITIS; PATIENT; COMPLICATIONS C1 [Chandana, Sreenivasa R.] West Michigan Canc Ctr, Kalamazoo, MI USA. [Kotecha, Rupesh] Michigan State Univ, Kalamazoo Ctr Med Studies, Kalamazoo, MI USA. [Al-Janadi, Anas] Michigan State Univ, Coll Human Med, E Lansing, MI 48824 USA. [Chang, Howard T.] Sparrow Hlth Syst, Lansing, MI USA. [Chang, Howard T.] Michigan State Univ, Coll Osteopath Med, E Lansing, MI 48824 USA. [Conley, Barbara A.] NCI, Rockville, MD USA. RP Chandana, SR (reprint author), West Michigan Canc Ctr, Kalamazoo, MI USA. NR 14 TC 5 Z9 5 U1 0 U2 2 PU AMER SOC CLINICAL ONCOLOGY PI ALEXANDRIA PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD APR 10 PY 2013 VL 31 IS 11 BP E186 EP E188 DI 10.1200/JCO.2012.44.8787 PG 3 WC Oncology SC Oncology GA 120MM UT WOS:000317174400006 PM 23439755 ER PT J AU Thanos, PK Robison, L Nestler, EJ Kim, R Michaelides, M Lobo, MK Volkow, ND AF Thanos, Panayotis K. Robison, Lisa Nestler, Eric J. Kim, Ronald Michaelides, Michael Lobo, Mary-Kay Volkow, Nora D. TI Mapping Brain Metabolic Connectivity in Awake Rats with mu PET and Optogenetic Stimulation SO JOURNAL OF NEUROSCIENCE LA English DT Article ID DEFAULT MODE NETWORK; OPIOID PEPTIDE RELEASE; NUCLEUS-ACCUMBENS; NALOXONE BLOCKS; PERIAQUEDUCTAL GRAY; GLUCOSE-METABOLISM; PREFRONTAL CORTEX; IN-VIVO; FUNCTIONAL CONNECTIVITY; SUBTHALAMIC NUCLEUS AB Positron emission tomography (PET) with [F-18]2-fluoro-2-deoxy-D-glucose was used to measure changes in regional brain glucose metabolism (BGluM) in response to optogenetic stimulation (using the excitatory channelrhodopsin-2) of the nucleus accumbens (NAc) in awake rats. We demonstrated not only increases in BGluM that correlated with c-Fos expression in the region of stimulation, but also BGluM increases in the ipsilateral striatum, periaqueductal gray, and somatosensory cortex, and in contralateral amygdala, ventral pallidum, globus pallidus, and hippocampus, as well as decreases in BGluM in regions of the default mode network (retrosplenial cortex and cingulate gyrus) and secondary motor cortex. Additional exploration of c-Fos expression in regions found to be activated by PET results found corroborating evidence, with increased c-Fos expression in the ipsilateral somatosensory cortex, contralateral amygdala and globus pallidus, and bilateral periaqueductal gray. These findings are consistent with optogenetic excitation of the area of stimulation (NAc), as well as with stimulatory and inhibitory effects on downstream regions. They also confirm the utility of PET imaging to monitor connectivity in the awake rodent brain. C1 [Thanos, Panayotis K.; Robison, Lisa; Kim, Ronald; Volkow, Nora D.] NIAAA, Lab Neuroimaging, NIH, Rockville, MD 20852 USA. [Thanos, Panayotis K.; Kim, Ronald] Brookhaven Natl Lab, Dept Med, Behav Neuropharmacol & Neuroimaging Lab, Upton, NY 11973 USA. [Thanos, Panayotis K.; Robison, Lisa] SUNY Stony Brook, Dept Psychol, Stony Brook, NY 11794 USA. [Lobo, Mary-Kay] Univ Maryland, Sch Med, Dept Anat & Neurobiol, Baltimore, MD 21201 USA. [Nestler, Eric J.; Michaelides, Michael] Mt Sinai Sch Med, Dept Neurosci, New York, NY 10029 USA. RP Lobo, MK (reprint author), Univ Maryland, Sch Med, 20 Penn St HSFII S251, Baltimore, MD 21201 USA. EM mklobo@umaryland.edu; nvolkow@nida.nih.gov RI Michaelides, Michael/K-4736-2013; Lobo, Mary Kay/K-7734-2015 OI Michaelides, Michael/0000-0003-0398-4917; Lobo, Mary Kay/0000-0002-9419-2079 FU intramural program at NIAAA [AA11034, AA07574, AA07611, DA08227] FX This work was supported by the intramural program at NIAAA and Grants AA11034, AA07574, AA07611, and DA08227. We thank Javier Gonzalez-Garzon, Manuel Desco, and Mala Ananth for helpful suggestions with the SPM. NR 60 TC 20 Z9 20 U1 0 U2 21 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD APR 10 PY 2013 VL 33 IS 15 BP 6343 EP 6349 DI 10.1523/JNEUROSCI.4997-12.2013 PG 7 WC Neurosciences SC Neurosciences & Neurology GA 124PE UT WOS:000317476300010 PM 23575833 ER PT J AU Matsubara, T Li, F Gonzalez, FJ AF Matsubara, Tsutomu Li, Fei Gonzalez, Frank J. TI FXR signaling in the enterohepatic system SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Review DE Nuclear receptors; Farnesoid X receptor; Bile acids; Cholestasis; Liver disease; Enterohepatic circulation ID FARNESOID-X-RECEPTOR; BILE-ACID TRANSPORTER; ORPHAN NUCLEAR RECEPTOR; SALT EXPORT PUMP; ORGANIC SOLUTE TRANSPORTER; FIBROBLAST-GROWTH-FACTOR; TAUROCHOLATE COTRANSPORTING POLYPEPTIDE; FAMILIAL INTRAHEPATIC CHOLESTASIS; NEGATIVE FEEDBACK-REGULATION; INFLAMMATORY-BOWEL-DISEASE AB Enterohepatic circulation serves to capture bile acids and other steroid metabolites produced in the liver and secreted to the intestine, for reabsorption back into the circulation and reuptake to the liver. This process is under tight regulation by nuclear receptor signaling. Bile acids, produced from cholesterol, can alter gene expression in the liver and small intestine via activating the nuclear receptors farnesoid X receptor (FXR; NR1H4), pregnane X receptor (PXR; NR1I2), vitamin D receptor (VDR; NR1I1), G protein coupled receptor TGR5, and other cell signaling pathways (JNK1/2, AKT and ERK1/2). Among these controls, FXR is known to be a major bile acid-responsive ligand-activated transcription factor and a crucial control element for maintaining bile acid homeostasis. FXR has a high affinity for several major endogenous bile acids, notably cholic acid, deoxycholic acid, chenodeoxycholic acid, and lithocholic acid. By responding to excess bile acids, FXR is a bridge between the liver and small intestine to control bile acid levels and regulate bile acid synthesis and enterohepatic flow. FXR is highly expressed in the liver and gut, relative to other tissues, and contributes to the maintenance of cholesterol/bile acid homeostasis by regulating a variety of metabolic enzymes and transporters. FXR activation also affects lipid and glucose metabolism, and can influence drug metabolism. Published by Elsevier Ireland Ltd. C1 [Matsubara, Tsutomu; Li, Fei; Gonzalez, Frank J.] NCI, Lab Metab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Gonzalez, FJ (reprint author), NCI, Lab Metab, Bldg 37,Room 3106, Bethesda, MD 20892 USA. EM gonzalef@mail.nih.gov RI Li, Fei/F-6849-2013 FU Intramural NIH HHS [ZIA BC005708-19] NR 151 TC 74 Z9 78 U1 1 U2 48 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD APR 10 PY 2013 VL 368 IS 1-2 SI SI BP 17 EP 29 DI 10.1016/j.mce.2012.05.004 PG 13 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 126PR UT WOS:000317633900002 PM 22609541 ER PT J AU Ansbro, MR Shukla, S Ambudkar, SV Yuspa, SH Li, LW AF Ansbro, Megan R. Shukla, Suneet Ambudkar, Suresh V. Yuspa, Stuart H. Li, Luowei TI Screening Compounds with a Novel High-Throughput ABCB1-Mediated Efflux Assay Identifies Drugs with Known Therapeutic Targets at Risk for Multidrug Resistance Interference SO PLOS ONE LA English DT Article ID HUMAN P-GLYCOPROTEIN; CELL-LINE; ABC TRANSPORTERS; INHIBITORS; CANCER; CARCINOMA; BINDING; EXPRESSION; PROTEIN; DOXORUBICIN AB ABCB1, also known as P-glycoprotein (P-gp) or multidrug resistance protein 1 (MDR1), is a membrane-associated multidrug transporter of the ATP-binding cassette (ABC) transporter family. It is one of the most widely studied transporters that enable cancer cells to develop drug resistance. Reliable high-throughput assays that can identify compounds that interact with ABCB1 are crucial for developing new therapeutic drugs. A high-throughput assay for measuring ABCB1-mediated calcein AM efflux was developed using a fluorescent and phase-contrast live cell imaging system. This assay demonstrated the time- and dose-dependent accumulation of fluorescent calcein in ABCB1-overexpressing KB-V1 cells. Validation of the assay was performed with known ABCB1 inhibitors, XR9576, verapamil, and cyclosporin A, all of which displayed dose-dependent inhibition of ABCB1-mediated calcein AM efflux in this assay. Phase-contrast and fluorescent images taken by the imaging system provided additional opportunities for evaluating compounds that are cytotoxic or produce false positive signals. Compounds with known therapeutic targets and a kinase inhibitor library were screened. The assay identified multiple agents as inhibitors of ABCB1-mediated efflux and is highly reproducible. Among compounds identified as ABCB1 inhibitors, BEZ235, BI 2536, IKK 16, and ispinesib were further evaluated. The four compounds inhibited calcein AM efflux in a dose-dependent manner and were also active in the flow cytometry-based calcein AM efflux assay. BEZ235, BI 2536, and IKK 16 also successfully inhibited the labeling of ABCB1 with radiolabeled photoaffinity substrate [I-125]iodoarylazidoprazosin. Inhibition of ABCB1 with XR9576 and cyclosporin A enhanced the cytotoxicity of BI 2536 to ABCB1-overexpressing cancer cells, HCT-15-Pgp, and decreased the IC50 value of BI 2536 by several orders of magnitude. This efficient, reliable, and simple high-throughput assay has identified ABCB1 substrates/inhibitors that may influence drug potency or drug-drug interactions and predict multidrug resistance in clinical treatment. C1 [Ansbro, Megan R.; Yuspa, Stuart H.; Li, Luowei] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. [Shukla, Suneet; Ambudkar, Suresh V.] NCI, Cell Biol Lab, Ctr Canc Res, Bethesda, MD 20892 USA. RP Li, LW (reprint author), NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. EM lilu@mail.nih.gov FU intramural program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research FX This work was supported by the intramural program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 18 Z9 18 U1 0 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 10 PY 2013 VL 8 IS 4 AR e60334 DI 10.1371/journal.pone.0060334 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 123IQ UT WOS:000317382000012 PM 23593196 ER PT J AU Metaferia, B Wei, JS Song, YK Evangelista, J Aschenbach, K Johansson, P Wen, XY Chen, QR Lee, A Hempel, H Gheeya, JS Getty, S Gomez, R Khan, J AF Metaferia, Belhu Wei, Jun S. Song, Young K. Evangelista, Jennifer Aschenbach, Konrad Johansson, Peter Wen, Xinyu Chen, Qingrong Lee, Albert Hempel, Heidi Gheeya, Jinesh S. Getty, Stephanie Gomez, Romel Khan, Javed TI Development of Peptide Nucleic Acid Probes for Detection of the HER2 Oncogene SO PLOS ONE LA English DT Article ID BREAST-CANCER; HYBRIDIZATION; PNA; DNA; IMMOBILIZATION; RESISTANCE; STABILITY; PNA/DNA; CELLS; RNA AB Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA. We have synthesized 15/16-mer PNA probes to detect the HER2 mRNA. The performance of these probes to detect the HER2 target was evaluated by fluorescence imaging and fluorescence bead assays. The PNA probes have sufficiently discriminated between the wild type HER2 target and the mutant target with single base mismatches. Furthermore, the probes exhibited excellent linear concentration dependence between 0.4 to 400 fmol for the target gene. The results demonstrate potential application of PNAs as diagnostic probes with high specificity for quantitative measurements of amplifications or over-expressions of oncogenes. C1 [Metaferia, Belhu; Wei, Jun S.; Song, Young K.; Johansson, Peter; Wen, Xinyu; Chen, Qingrong; Lee, Albert; Hempel, Heidi; Gheeya, Jinesh S.; Khan, Javed] NCI, Pediat Oncol Branch, NIH, Bethesda, MD 20892 USA. [Wen, Xinyu] NCI, Adv Biomed Comp Ctr, SAIC Frederick Inc, Frederick, MD 21701 USA. [Evangelista, Jennifer; Aschenbach, Konrad; Gomez, Romel] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA. [Getty, Stephanie] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. RP Khan, J (reprint author), NCI, Pediat Oncol Branch, NIH, Bethesda, MD 20892 USA. EM khanjav@mail.nih.gov RI Getty, Stephanie/D-7037-2012; Khan, Javed/P-9157-2014; Johansson, Peter/K-1053-2014; OI Khan, Javed/0000-0002-5858-0488; Johansson, Peter/0000-0001-7015-5452; Gheeya, Jinesh/0000-0002-5246-6262 FU National Institutes of Health, National Cancer Institute, Center for Cancer Research FX This study was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 31 TC 1 Z9 1 U1 0 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 10 PY 2013 VL 8 IS 4 AR e58870 DI 10.1371/journal.pone.0058870 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 123IQ UT WOS:000317382000002 PM 23593123 ER PT J AU Schountz, T Shaw, TI Glenn, TC Feldmann, H Prescott, J AF Schountz, Tony Shaw, Timothy I. Glenn, Travis C. Feldmann, Heinz Prescott, Joseph TI Expression profiling of lymph node cells from deer mice infected with Andes virus SO BMC IMMUNOLOGY LA English DT Article ID SIN-NOMBRE-VIRUS; MOUSE PEROMYSCUS-MANICULATUS; HUMAN GRANULOCYTIC EHRLICHIOSIS; VESICULAR STOMATITIS-VIRUS; GERMINAL CENTER B; BORRELIA-BURGDORFERI; NORTH-AMERICA; DIFFERENTIATION; HANTAVIRUS; LEUCOPUS AB Background: Deer mice (Peromyscus maniculatus) are the principal reservoir hosts of Sin Nombre virus (SNV), the cause of the great majority of hantavirus cardiopulmonary syndrome (HCPS) cases in North America. SNV, like all hantaviruses with their reservoirs, causes persistent infection without pathology in deer mice and appear to elicit a regulatory T cell response. Deer mice are also susceptible to Andes virus (ANDV), which causes the great majority of HCPS cases in South America, but they clear infection by 56 days post infection without signs of disease. Results: We examined lymph node cell responses of deer mice infected with ANDV to determine expression profiles upon in vitro recall challenge with viral antigen. Because the deer mouse genome is currently unannotated, we developed a bioinformatics pipeline to use known lab mouse (Mus musculus) cDNAs to predict genes within the deer mouse genome and design primers for quantitative PCR (http://dna.publichealth.uga.edu/BlastPrimer/BlastPrimer.php). Of 94 genes examined, 20 were elevated, the plurality of which were Th2-specific, whereas 12 were downregulated. Other expressed genes represented Th1, regulatory T cells and follicular helper T cells, and B cells, but not Th17 cells, indicating that many cellular phenotypes participate in the host response to Andes virus. Conclusions: The ability to examine expression levels of nearly any gene from deer mice should allow direct comparison of infection with SNV or ANDV to determine the immunological pathways used for clearance of hantavirus infection in a reservoir host species. C1 [Schountz, Tony] Colorado State Univ, Coll Vet Med, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Shaw, Timothy I.; Glenn, Travis C.] Univ Georgia, Inst Bioinformat, Athens, GA 30602 USA. [Glenn, Travis C.] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 USA. [Feldmann, Heinz; Prescott, Joseph] NIAID, Virol Lab, Div Intramural Res, NIH,Rocky Mt Labs, Hamilton, MT USA. RP Schountz, T (reprint author), Colorado State Univ, Coll Vet Med, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. EM tony.schountz@colostate.edu FU NIH [AI054461]; Intramural Program of NIAID, NIH; University of Georgia Advanced Computing Resource Center, Institute of Bioinformatics; ARCS Foundation FX We thank Rebecca Staudenmaier for downloading house mouse cDNA sequences, Rachel LaCasse for assistance with collection of lymph nodes from infected deer mice and Brian Hjelle for supplying recombinant ANDV nucleocapsid antigen. This work was supported by NIH grant AI054461 (to T. S.), the Intramural Program of NIAID, NIH (H.F., J.P.), the University of Georgia Advanced Computing Resource Center, Institute of Bioinformatics (T.I.S., T.G.), and the ARCS Foundation (T.I.S). NR 41 TC 12 Z9 12 U1 1 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2172 J9 BMC IMMUNOL JI BMC Immunol. PD APR 9 PY 2013 VL 14 AR 18 DI 10.1186/1471-2172-14-18 PG 12 WC Immunology SC Immunology GA 140MJ UT WOS:000318657900001 PM 23570545 ER PT J AU Lahrmann, B Valous, NA Eisenmann, U Wentzensen, N Grabe, N AF Lahrmann, Bernd Valous, Nektarios A. Eisenmann, Urs Wentzensen, Nicolas Grabe, Niels TI Semantic Focusing Allows Fully Automated Single-Layer Slide Scanning of Cervical Cytology Slides SO PLOS ONE LA English DT Article ID LIQUID-BASED CYTOLOGY; SUPPORT VECTOR MACHINES; HUMAN-PAPILLOMAVIRUS; CANCER; PERFORMANCE; COLPOSCOPY; SYSTEMS AB Liquid-based cytology (LBC) in conjunction with Whole-Slide Imaging (WSI) enables the objective and sensitive and quantitative evaluation of biomarkers in cytology. However, the complex three-dimensional distribution of cells on LBC slides requires manual focusing, long scanning-times, and multi-layer scanning. Here, we present a solution that overcomes these limitations in two steps: first, we make sure that focus points are only set on cells. Secondly, we check the total slide focus quality. From a first analysis we detected that superficial dust can be separated from the cell layer (thin layer of cells on the glass slide) itself. Then we analyzed 2,295 individual focus points from 51 LBC slides stained for p16 and Ki67. Using the number of edges in a focus point image, specific color values and size-inclusion filters, focus points detecting cells could be distinguished from focus points on artifacts (accuracy 98.6%). Sharpness as total focus quality of a virtual LBC slide is computed from 5 sharpness features. We trained a multi-parameter SVM classifier on 1,600 images. On an independent validation set of 3,232 cell images we achieved an accuracy of 94.8% for classifying images as focused. Our results show that single-layer scanning of LBC slides is possible and how it can be achieved. We assembled focus point analysis and sharpness classification into a fully automatic, iterative workflow, free of user intervention, which performs repetitive slide scanning as necessary. On 400 LBC slides we achieved a scanning-time of 13.9 +/- 10.1 min with 29.1 +/- 15.5 focus points. In summary, the integration of semantic focus information into whole-slide imaging allows automatic high-quality imaging of LBC slides and subsequent biomarker analysis. C1 [Lahrmann, Bernd; Valous, Nektarios A.; Grabe, Niels] Heidelberg Univ, Hamamatsu Tissue Imaging & Anal Ctr TIGA, BIOQUANT, Heidelberg, Germany. [Lahrmann, Bernd; Valous, Nektarios A.; Grabe, Niels] Heidelberg Univ, Univ Heidelberg Hosp, Natl Ctr Tumor Dis, Heidelberg, Germany. [Wentzensen, Nicolas] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Eisenmann, Urs] Univ Heidelberg Hosp, Inst Med Biometry & Informat, Heidelberg, Germany. [Lahrmann, Bernd] Univ Heidelberg Hosp, Inst Pathol, Heidelberg, Germany. RP Grabe, N (reprint author), Heidelberg Univ, Hamamatsu Tissue Imaging & Anal Ctr TIGA, BIOQUANT, Heidelberg, Germany. EM niels.grabe@bioquant.uni-heidelberg.de OI Valous, Nektarios/0000-0002-4014-2404 FU German Ministry for Research and Education (BMBF) [0315401B, 0315263]; Intramural Research Program of the National Cancer Institute FX Funding was provided by the German Ministry for Research and Education (BMBF) in their MEDSYS and FORSYS funding programs, Grant Numbers 0315401B (MEDSYS), 0315263 (FORSYS). The work was in part supported by the Intramural Research Program of the National Cancer Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 4 Z9 4 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2013 VL 8 IS 4 AR e61441 DI 10.1371/journal.pone.0061441 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130JB UT WOS:000317909600114 PM 23585899 ER PT J AU Parikh, UM Kiepiela, P Ganesh, S Gomez, K Horn, S Eskay, K Kelly, C Mensch, B Gorbach, P Soto-Torres, L Ramjee, G Mellors, JW AF Parikh, Urvi M. Kiepiela, Photini Ganesh, Shayhana Gomez, Kailazarid Horn, Stephanie Eskay, Krista Kelly, Cliff Mensch, Barbara Gorbach, Pamina Soto-Torres, Lydia Ramjee, Gita Mellors, John W. CA MTN-Protocol Team TI Prevalence of HIV-1 Drug Resistance among Women Screening for HIV Prevention Trials in KwaZulu-Natal, South Africa (MTN-009) SO PLOS ONE LA English DT Article ID SURVEILLANCE; WORLD; MUTATIONS; ADHERENCE; ROLLOUT; UPDATE AB Background: A major concern with using antiretroviral (ARV)-based products for HIV prevention is the potential spread of drug resistance, particularly from individuals who are HIV-infected but unaware of their status. Limited data exist on the prevalence of HIV infection or drug resistance among potential users of ARV-based prevention products. Methods: A cross-sectional study of reproductive-aged women who presented to screen for an HIV prevention trial was conducted at 7 clinical sites in Durban, South Africa. CD4+ T cell counts, HIV-1 RNA levels and population sequencing of the protease and reverse transcriptase genes were performed for all women with 2 positive HIV rapid tests. Resistance mutations were identified using the Stanford Calibrated Population Resistance Tool. Results: Of the 1073 evaluable women, 400(37%) were confirmed as HIV-infected. Of those, plasma HIV-1 RNA was detectable in 365/400(91%) and undetectable(<40 copies/ml) in 35/400(9%) women. 156 women(39%) were eligible for antiretroviral therapy (CD4+ T cell counts<350 cells/mm(3)) and 50(13%) met criteria for AIDS(CD4<200 cells/mm(3)). Of 352 plasma samples(>200 copies/ml) analyzed for drug resistance, 26(7.4%) had nucleoside reverse transcriptase inhibitor (NRTI), non-nucleoside reverse transcriptase inhibitor (NNRTI) or protease inhibitor (PI) drug resistance mutations. Among those with resistance, 18/26 participants(62%) had single-class NNRTI resistance and 5/26(19%) had dual-class NRTI/NNRTI. Major mutations in reverse transcriptase included K65R(n = 1), L74I(n = 1), K103N(n = 19), V106M(n = 4), Y181C(n = 2), M184V(n = 4), and K219E/R(n = 2). Major PI-resistance mutations were rare: M46L(n = 1) and I85V(n = 1). All participants were infected with subtype C virus, except one infected with subtype A. Conclusions: In women from Durban, South Africa screening for an HIV prevention trial, the HIV prevalence was high (37%) and HIV drug resistance prevalence was above 5%. This study highlights the potential challenges faced when implementing an ARV-based prevention product that overlaps with first-line antiretroviral therapy. Effective screening to exclude HIV infection among women interested in uptake of ARV-based HIV prevention will be essential in limiting the spread of ARV resistance. C1 [Parikh, Urvi M.; Eskay, Krista; Mellors, John W.] Univ Pittsburgh, Sch Med, Dept Infect Dis, Pittsburgh, PA 15260 USA. [Kiepiela, Photini; Ganesh, Shayhana; Ramjee, Gita] MRC, HIV Prevent Res Unit, Durban, South Africa. [Gomez, Kailazarid; Horn, Stephanie] FHI 360, Res Triangle Pk, NC USA. [Kelly, Cliff] Fred Hutchinson Canc Res Ctr, Stat Ctr HIV AIDS Res & Prevent, Seattle, WA 98104 USA. [Mensch, Barbara] Populat Council, New York, NY 10021 USA. [Gorbach, Pamina] Univ Calif Los Angeles, Dept Epidemiol, Los Angeles, CA USA. [Soto-Torres, Lydia] NIAID, Div Aids, NIH, Bethesda, MD 20892 USA. RP Parikh, UM (reprint author), Univ Pittsburgh, Sch Med, Dept Infect Dis, Pittsburgh, PA 15260 USA. EM ump3@pitt.edu FU National Institute of Allergy and Infectious Diseases, component of the U.S. National Institutes of Health [5UM1AI068633]; National Institute of Child Health and Human Development, component of the U.S. National Institutes of Health FX The Microbicide Trials Network is funded by the National Institute of Allergy and Infectious Diseases (5UM1AI068633), with co-funding from the National Institute of Child Health and Human Development, all components of the U.S. National Institutes of Health. As a US Public Health Service Commissioned Corps Officer in the Division of AIDS (DAIDS) at the National Institute of Allergy and Infectious Diseases (NIAID), Dr. Soto-Torres represents the funder (NIAID). However, she has no official oversight of MTN funding or budgetary issues. Her responsibilities as a DAIDS Medical Officer are to oversee the design and conduct of MTN-009 as well as monitor participant safety in the study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 33 TC 12 Z9 12 U1 1 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2013 VL 8 IS 4 AR e59787 DI 10.1371/journal.pone.0059787 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130JB UT WOS:000317909600009 PM 23585827 ER PT J AU Zhang, WR Davis, CM Edin, ML Lee, CR Zeldin, DC Alkayed, NJ AF Zhang, Wenri Davis, Catherine M. Edin, Matthew L. Lee, Craig R. Zeldin, Darryl C. Alkayed, Nabil J. TI Role of Endothelial Soluble Epoxide Hydrolase in Cerebrovascular Function and Ischemic Injury SO PLOS ONE LA English DT Article ID EPOXYEICOSATRIENOIC ACIDS; CORONARY-ARTERIES; CEREBRAL-ISCHEMIA; SEX-DIFFERENCES; EPOXYGENASE; STROKE; BRAIN; EPOXYEICOSANOIDS; NEUROPROTECTION; VASODILATION AB Soluble Epoxide Hydrolase (sEH) is a key enzyme in the metabolism and termination of action of epoxyeicosatrienoic acids, derivatives of arachidonic acid, which are protective against ischemic stroke. Mice lacking sEH globally are protected from injury following stroke; however, little is known about the role of endothelial sEH in brain ischemia. We generated transgenic mice with endothelial-specific expression of human sEH (Tie2-hsEH), and assessed the effect of transgenic overexpression of endothelial sEH on endothelium-dependent vascular reactivity and ischemic injury following middle cerebral artery occlusion (MCAO). Compared to wild-type, male Tie2-hsEH mice exhibited impaired vasodilation in response to stimulation with 1 mu M acetylcholine as assessed by laser-Doppler perfusion monitoring in an in-vivo cranial window preparation. No difference in infarct size was observed between wild-type and Tie2-hsEH male mice. In females, however, Tie2-hsEH mice sustained larger infarcts in striatum, but not cortex, compared to wild-type mice. Sex difference in ischemic injury was maintained in the cortex of Tie2-hsEH mice. In the striatum, expression of Tie2-hsEH resulted in a sex difference, with larger infarct in females than males. These findings demonstrate that transgenic expression of sEH in endothelium results in impaired endothelium-dependent vasodilation in the cerebral circulation, and that females are more susceptible to enhanced ischemic damage as a result of increased endothelial sEH than males, especially in end-arteriolar striatal region. C1 [Zhang, Wenri; Davis, Catherine M.; Alkayed, Nabil J.] Oregon Hlth & Sci Univ, Dept Anesthesiol & Perioperat Med, Portland, OR 97201 USA. [Edin, Matthew L.; Zeldin, Darryl C.] NIEHS, Div Intramural Res, NIH, Res Triangle Pk, NC 27709 USA. [Lee, Craig R.] Univ N Carolina, Eshelman Sch Pharm, Div Pharmacotherapy & Expt Therapeut, Chapel Hill, NC USA. RP Alkayed, NJ (reprint author), Oregon Hlth & Sci Univ, Dept Anesthesiol & Perioperat Med, Portland, OR 97201 USA. EM alkayen@ohsu.edu OI Edin, Matthew/0000-0002-7042-500X; Lee, Craig/0000-0003-3595-5301 FU Division of Intramural Research of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences [NIH Z01 ES025034]; AHA; NIH [T32 GM082770-03, R01 NS044313, NS070837] FX This manuscript was supported in part by the Division of Intramural Research of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences, NIH Z01 ES025034 to DCZ (http://grants.nih.gov/grants/oer.htm); AHA postdoctoral fellowship to CMD (http://my.americanheart.org/professional/Research/Research_UCM_316889_S ubHomePage.jsp); NIH T32 GM082770-03, NIH R01 NS044313 and NS070837 to NJA, and NIH R01 to CRL (http://grants.nih.gov/grants/oer.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 28 TC 12 Z9 12 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2013 VL 8 IS 4 AR e61244 DI 10.1371/journal.pone.0061244 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130JB UT WOS:000317909600096 PM 23585883 ER PT J AU Best, RB AF Best, Robert B. TI A "slow" protein folds quickly in the end SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Editorial Material ID TRANSITION PATH TIMES; BETA-HAIRPIN; FORCE-FIELD; SIMULATIONS; STATE; DYNAMICS C1 NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Best, RB (reprint author), NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. EM robertbe@helix.nih.gov RI Best, Robert/H-7588-2016 OI Best, Robert/0000-0002-7893-3543 FU Intramural NIH HHS NR 23 TC 1 Z9 1 U1 1 U2 26 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP 5744 EP 5745 DI 10.1073/pnas.1303539110 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125KE UT WOS:000317537900006 PM 23572570 ER PT J AU Schauder, DM Kuybeda, O Zhang, JJ Klymko, K Bartesaghi, A Borgnia, MJ Mayer, ML Subramaniam, S AF Schauder, David M. Kuybeda, Oleg Zhang, Jinjin Klymko, Katherine Bartesaghi, Alberto Borgnia, Mario J. Mayer, Mark L. Subramaniam, Sriram TI Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE cryoelectron microscopy; ion channel gating ID SIZE-EXCLUSION CHROMATOGRAPHY; AMINO-TERMINAL DOMAINS; AMPA RECEPTOR; CRYSTAL-STRUCTURES; CRYOELECTRON TOMOGRAPHY; 3-DIMENSIONAL STRUCTURE; CONFORMATIONAL-CHANGES; ELECTRON TOMOGRAPHY; KAINATE RECEPTORS; NMDA RECEPTORS AB Glutamate receptor ion channels are membrane proteins that mediate excitatory synaptic transmission in the central nervous system of vertebrates. Insight into molecular mechanisms underlying glutamate receptor gating is limited by lack of structural information for receptors trapped in different conformational states. Here, we report the use of single-particle cryoelectron tomography to determine the structures, at similar to 21 angstrom resolution, of full-length GluK2 kainate receptors trapped in antagonist-bound resting and agonist-bound desensitized states. The resting state, stabilized by the competitive antagonist LY466195, closely resembles the crystal structure of the AMPA receptor GluA2, with well-resolved proximal and distal subunits exhibiting cross-over between the twofold symmetric amino terminal domain and a twofold symmetric ligand binding domain (LBD) dimer of dimers assembly. In the desensitized state, the LBD undergoes a major rearrangement, resulting in a separation of the four subunits by similar to 25 angstrom. However, the amino terminal domain, transmembrane, and cytoplasmic regions of the receptor have similar conformations in the resting and desensitized states. The LBD rearrangement was not anticipated in prior models based on crystal structures for soluble LBD dimer assemblies, and we speculate that subunit separation allows a better match to the fourfold symmetric ion channel domain. From fits of the amino terminal domain and LBD domains into the density map of the desensitized state we have derived a structural model for differences in quaternary conformation between the resting and desensitized states. C1 [Schauder, David M.; Klymko, Katherine; Bartesaghi, Alberto; Borgnia, Mario J.; Subramaniam, Sriram] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Kuybeda, Oleg] NICHHD, Natl Lib Med, NIH, Bethesda, MD 20892 USA. [Zhang, Jinjin; Mayer, Mark L.] NICHHD, Lab Cellular & Mol Neurophysiol, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA. RP Mayer, ML (reprint author), NICHHD, Lab Cellular & Mol Neurophysiol, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA. EM mayerm@mail.nih.gov; ss1@nih.gov RI Mayer, Mark/H-5500-2013 FU Center for Cancer Research, National Cancer Institute, National Institutes of Health (NIH); National Institute of Child Health and Human Development, NIH, Department of Health and Human Services FX We thank Eric Gouaux and members of his laboratory for advice on membrane protein expression and purification, Dr. David Bleakman (Eli Lilly and Company) for the gift of LY466195, and Dr: Haifeng He for advice on electron microscopic data collection. This work was supported by funds from the Center for Cancer Research, National Cancer Institute, National Institutes of Health (NIH) (to S.S.) and from the intramural research program of the National Institute of Child Health and Human Development, NIH, Department of Health and Human Services (to M.L.M.). NR 48 TC 32 Z9 32 U1 1 U2 19 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP 5921 EP 5926 DI 10.1073/pnas.1217549110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125KE UT WOS:000317537900041 PM 23530186 ER PT J AU Killela, PJ Reitman, ZJ Jiao, YC Bettegowda, C Agrawal, N Diaz, LA Friedman, AH Friedman, H Gallia, GL Giovanella, BC Grollman, AP He, TC He, YP Hruban, RH Jallo, GI Mandahl, N Meeker, AK Mertens, F Netto, GJ Rasheed, BA Riggins, GJ Rosenquist, TA Schiffman, M Shih, IM Theodorescu, D Torbenson, MS Velculescu, VE Wang, TL Wentzensen, N Wood, LD Zhang, M McLendon, RE Bigner, DD Kinzler, KW Vogelstein, B Papadopoulos, N Yan, H AF Killela, Patrick J. Reitman, Zachary J. Jiao, Yuchen Bettegowda, Chetan Agrawal, Nishant Diaz, Luis A., Jr. Friedman, Allan H. Friedman, Henry Gallia, Gary L. Giovanella, Beppino C. Grollman, Arthur P. He, Tong-Chuan He, Yiping Hruban, Ralph H. Jallo, George I. Mandahl, Nils Meeker, Alan K. Mertens, Fredrik Netto, George J. Rasheed, B. Ahmed Riggins, Gregory J. Rosenquist, Thomas A. Schiffman, Mark Shih, Ie-Ming Theodorescu, Dan Torbenson, Michael S. Velculescu, Victor E. Wang, Tian-Li Wentzensen, Nicolas Wood, Laura D. Zhang, Ming McLendon, Roger E. Bigner, Darell D. Kinzler, Kenneth W. Vogelstein, Bert Papadopoulos, Nickolas Yan, Hai TI TERT promoter mutations occur frequently in gliomas and a subset of tumors derived from cells with low rates of self-renewal SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID HUMAN TELOMERASE ACTIVITY; HEPATOCELLULAR-CARCINOMA; MEDULLOBLASTOMA SUBTYPES; HUMAN BREAST; HUMAN CANCER; GENES; ASSOCIATION; MAINTENANCE; PROGRESSION; DIFFERENTIATION AB Malignant cells, like all actively growing cells, must maintain their telomeres, but genetic mechanisms responsible for telomere maintenance in tumors have only recently been discovered. In particular, mutations of the telomere binding proteins alpha thalassemia/mental retardation syndrome X-linked (ATRX) or death-domain associated protein (DAXX) have been shown to underlie a telomere maintenance mechanism not involving telomerase (alternative lengthening of telomeres), and point mutations in the promoter of the telomerase reverse transcriptase (TERT) gene increase telomerase expression and have been shown to occur in melanomas and a small number of other tumors. To further define the tumor types in which this latter mechanism plays a role, we surveyed 1,230 tumors of 60 different types. We found that tumors could be divided into types with low (<15%) and high (>= 15%) frequencies of TERT promoter mutations. The nine TERT-high tumor types almost always originated in tissues with relatively low rates of self renewal, including melanomas, liposarcomas, hepatocellular carcinomas, urothelial carcinomas, squamous cell carcinomas of the tongue, medulloblastomas, and subtypes of gliomas (including 83% of primary glioblastoma, the most common brain tumor type). TERT and ATRX mutations were mutually exclusive, suggesting that these two genetic mechanisms confer equivalent selective growth advantages. In addition to their implications for understanding the relationship between telomeres and tumorigenesis, TERT mutations provide a biomarker that may be useful for the early detection of urinary tract and liver tumors and aid in the classification and prognostication of brain tumors. C1 [Killela, Patrick J.; Reitman, Zachary J.; Friedman, Allan H.; Friedman, Henry; He, Yiping; Rasheed, B. Ahmed; McLendon, Roger E.; Bigner, Darell D.; Yan, Hai] Duke Univ, Pediat Brain Tumor Fdn Inst Duke, Preston Robert Tisch Brain Tumor Ctr Duke, Durham, NC 27710 USA. [Jiao, Yuchen; Bettegowda, Chetan; Agrawal, Nishant; Diaz, Luis A., Jr.; Velculescu, Victor E.; Zhang, Ming; Kinzler, Kenneth W.; Vogelstein, Bert; Papadopoulos, Nickolas] Johns Hopkins Med Inst, Ludwig Ctr Canc Genet, Baltimore, MD 21231 USA. [Jiao, Yuchen; Bettegowda, Chetan; Agrawal, Nishant; Diaz, Luis A., Jr.; Velculescu, Victor E.; Zhang, Ming; Kinzler, Kenneth W.; Vogelstein, Bert; Papadopoulos, Nickolas] Johns Hopkins Med Inst, Johns Hopkins Kimmel Canc Ctr, Howard Hughes Med Inst, Baltimore, MD 21231 USA. [Bettegowda, Chetan; Gallia, Gary L.; Jallo, George I.; Riggins, Gregory J.] Johns Hopkins Univ, Sch Med, Dept Neurosurg, Baltimore, MD 21231 USA. [Agrawal, Nishant; Gallia, Gary L.] Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Baltimore, MD 21231 USA. [Hruban, Ralph H.; Meeker, Alan K.; Netto, George J.; Shih, Ie-Ming; Torbenson, Michael S.; Wang, Tian-Li; Wood, Laura D.] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21231 USA. [Netto, George J.] Johns Hopkins Univ, Sch Med, Dept Urol, Baltimore, MD 21231 USA. [Meeker, Alan K.] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21231 USA. [Giovanella, Beppino C.] Christus Stehlin Fdn Canc Res, Houston, TX 77025 USA. [Grollman, Arthur P.; Rosenquist, Thomas A.] SUNY Stony Brook, Dept Pharmacol Sci, Stony Brook, NY 11794 USA. [He, Tong-Chuan] Univ Chicago, Med Ctr, Dept Orthopaed Surg, Mol Oncol Lab, Chicago, IL 60637 USA. [Mandahl, Nils; Mertens, Fredrik] Univ Lund Hosp, Dept Clin Genet, S-22185 Lund, Sweden. [Schiffman, Mark; Wentzensen, Nicolas] NCI, Div Canc Epidemiol & Genet, NIH, Rockville, MD 20852 USA. [Theodorescu, Dan] Univ Colorado, Ctr Comprehens Canc, Aurora, CO 80045 USA. RP Vogelstein, B (reprint author), Johns Hopkins Med Inst, Ludwig Ctr Canc Genet, Baltimore, MD 21231 USA. EM bertvog@gmail.com; yan00002@mc.duke.edu RI Waha, Andreas/J-2950-2014; OI Mertens, Fredrik/0000-0002-6278-5232 FU American Cancer Society Research Scholar Award [RSG-10-126-01-CCE]; National Cancer Institute Grant [5R01-CA140316]; Pediatric Brain Tumor Foundation Institute grant; Voices Against Brain Cancer Foundation grant; James S. McDonnell Foundation grant; V Foundation; Accelerate Brain Cancer Cure Foundation grant; Burroughs Wellcome Career Award for Medical Scientists; Johns Hopkins Clinician Scientist Award; BSi Brain Science Translational Research grant; Malia's Cord Foundation Grant; Zickler Family Foundation; Swim Across America; Swedish Childhood Cancer Foundation; Flight Attendant Medical Research Institute (FAMRI); FAMRI Clinical Innovator Award; Lustgarten Foundation; Sol Goldman Pancreatic Cancer Research Center; Virginia and D. K. Ludwig Fund for Cancer Research; National Institutes of Health Grant: Intramural Research Program of the National Cancer Institute; National Institutes of Health Grant: National Institute of Neurological Disorders and Stroke Grant [5P50 NS20023]; National Institutes of Health Grant: National Cancer Institute Specialized Program of Research Excellence [5P50 CA108785, CA121113, CA075115, CA104106, CA057345, CA129825, CA43460, CA57345, CA62924, P50DE019032, ES04068, CA172380]; National Institutes of Health Grant: National Cancer Institute Contract [N01-CN-43309]; National Institutes of Health Grant: National Cancer Institute Merit Award [R37 011898] FX We thank Lisa J. Ehinger and Diane L. Satterfield for collecting clinical samples. We also thank David Lister for 1p/19q analysis and J. Ptak, N. Silliman, L. Dobbyn, B. Bartlett, and J. Schaeffer for expert technical assistance. This project was supported by American Cancer Society Research Scholar Award RSG-10-126-01-CCE, National Cancer Institute Grant 5R01-CA140316, a Pediatric Brain Tumor Foundation Institute grant, a Voices Against Brain Cancer Foundation grant, a James S. McDonnell Foundation grant, The V Foundation, an Accelerate Brain Cancer Cure Foundation grant, The Burroughs Wellcome Career Award for Medical Scientists, The Johns Hopkins Clinician Scientist Award, a BSi Brain Science Translational Research grant, Malia's Cord Foundation Grant, The Zickler Family Foundation, Swim Across America, The Swedish Childhood Cancer Foundation, The Flight Attendant Medical Research Institute (FAMRI), FAMRI Clinical Innovator Award 2009, The Lustgarten Foundation, The Sol Goldman Pancreatic Cancer Research Center, and The Virginia and D. K. Ludwig Fund for Cancer Research. This work was also supported by the following National Institutes of Health Grants: Intramural Research Program of the National Cancer Institute, National Institute of Neurological Disorders and Stroke Grant 5P50 NS20023, National Cancer Institute Specialized Program of Research Excellence Grants 5P50 CA108785, CA121113, CA075115, CA104106, CA057345, CA129825, CA43460, CA57345, CA62924, P50DE019032, and ES04068, and CA172380, National Cancer Institute Contract N01-CN-43309, and National Cancer Institute Merit Award R37 011898. NR 46 TC 397 Z9 411 U1 5 U2 67 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP 6021 EP 6026 DI 10.1073/pnas.1303607110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125KE UT WOS:000317537900058 PM 23530248 ER PT J AU Lin, DC Xu, L Ding, LW Sharma, A Liu, LZ Yang, H Tan, P Vadgama, J Karlan, BY Lester, J Urban, N Schummer, M Doan, N Said, JW Sun, HM Walsh, M Thomas, CJ Patel, P Yin, D Chan, D Koeffler, HP AF Lin, De-Chen Xu, Liang Ding, Ling-Wen Sharma, Arjun Liu, Li-Zhen Yang, Henry Tan, Patrick Vadgama, Jay Karlan, Beth Y. Lester, Jenny Urban, Nicole Schummer, Michel Ngan Doan Said, Jonathan W. Sun, Hongmao Walsh, Martin Thomas, Craig J. Patel, Paresma Yin, Dong Chan, Daniel Koeffler, H. Philip TI Genomic and functional characterizations of phosphodiesterase subtype 4D in human cancers SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID CAMP-SPECIFIC PHOSPHODIESTERASE; CYCLIC-AMP; MALIGNANT-MELANOMA; CELLS; EXPRESSION; KINASE; PHOSPHORYLATION; LEUKEMIA; IDENTIFICATION; INHIBITION AB Discovery of cancer genes through interrogation of genomic dosage is one of the major approaches in cancer research. In this study, we report that phosphodiesterase subtype 4D (PDE4D) gene was homozygously deleted in 198 cases of 5,569 primary solid tumors (3.56%), with most being internal microdeletions. Unexpectedly, the microdeletions did not result in loss of their gene products. Screening PDE4D expression in 11 different types of primary tumor samples (n = 165) with immunohistochemistry staining revealed that its protein levels were up-regulated compared with corresponding non-transformed tissues. Importantly, depletion of endogenous PDE4D with three independent shRNAs caused apoptosis and growth inhibition in multiple types of cancer cells, including breast, lung, ovary, endometrium, gastric, and melanoma, which could be rescued by reexpression of PDE4D. We further showed that antitumor events triggered by PDE4D suppression were lineage-dependently associated with Bcl-2 interacting mediator of cell death (BIM) induction and microphthalmia-associated transcription factor (MITF) down-regulation. Furthermore, ectopic expression of the PDE4D short isoform, PDE4D2, enhanced the proliferation of cancer cells both in vitro and in vivo. Moreover, treatment of cancer cells with a unique specific PDE4D inhibitor, 268, triggered massive cell death and growth retardation. Notably, these antineoplastic effects induced by either shRNAs or small molecule occurred preferentially in cancer cells but not in nonmalignant epithelial cells. These results suggest that although targeted by genomic homozygous microdeletions, PDE4D functions as a tumor-promoting factor and represents a unique targetable enzyme of cancer cells. C1 [Lin, De-Chen; Koeffler, H. Philip] Univ Calif Los Angeles, Sch Med, Cedars Sinai Med Ctr, Div Hematol Oncol, Los Angeles, CA 90048 USA. [Lin, De-Chen; Xu, Liang; Ding, Ling-Wen; Sharma, Arjun; Liu, Li-Zhen; Yang, Henry; Tan, Patrick; Koeffler, H. Philip] Natl Univ Singapore, Canc Sci Inst Singapore, Singapore 117599, Singapore. [Vadgama, Jay] Charles R Drew Univ Med & Sci, Los Angeles, CA 90059 USA. [Karlan, Beth Y.; Lester, Jenny] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Womens Canc Program, Los Angeles, CA 90048 USA. [Urban, Nicole; Schummer, Michel] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA. [Sun, Hongmao; Walsh, Martin; Thomas, Craig J.] Santa Monica Univ Calif, Los Angeles Med Ctr, Natl Ctr Adv Translat Sci, Los Angeles, CA 90404 USA. [Sun, Hongmao; Walsh, Martin; Thomas, Craig J.] NIH, Chem Genom Ctr, Natl Ctr Adv Translat Sci, Bethesda, MD 20892 USA. [Patel, Paresma] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Biol Chem Lab, Basic Sci Program, Frederick, MD 21702 USA. [Yin, Dong] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Med Res Ctr, Guangdong Higher Educ Inst,Key Lab Malignant Tumo, Guangzhou 510120, Guangdong, Peoples R China. [Chan, Daniel; Koeffler, H. Philip] Natl Univ Singapore Hosp, Natl Univ Canc Inst, Singapore 117599, Singapore. RP Lin, DC (reprint author), Univ Calif Los Angeles, Sch Med, Cedars Sinai Med Ctr, Div Hematol Oncol, Los Angeles, CA 90048 USA. EM dchlin11@gmail.com RI LIN, DECHEN/K-9457-2014; OI Ding, Ling-Wen/0000-0003-0022-1551 FU Frederick National Laboratory for Cancer Research; National Institutes of Health (NIH) [HHSN261200800001E, R01CA026038-32, U54CA143930, 2P01 HL073104-06]; Singapore Ministry of Health's National Medical Research Council (NMRC) under its Singapore Translational Research (STaR) Investigator Award; Singapore NMRC New Investigator Grant; National Research Foundation Singapore; Singapore Ministry of Education under the Research Centres of Excellence initiative FX For triple negative breast cancer samples, the authors thank Eng Chon Boon (National University Health System, Singapore) and Kathy O'Briant (Fred Hutchinson Cancer Research Center). The 26B study is funded in part with federal funds from the Frederick National Laboratory for Cancer Research, and National Institutes of Health (NIH) under Contract HHSN261200800001E. This work is supported by NIH Grants R01CA026038-32, U54CA143930, and 2P01 HL073104-06; the Singapore Ministry of Health's National Medical Research Council (NMRC) under its Singapore Translational Research (STaR) Investigator Award (to H.P.K.); and a Singapore NMRC New Investigator Grant 2012 (to D.C.). This research is supported by the National Research Foundation Singapore and the Singapore Ministry of Education under the Research Centres of Excellence initiative. NR 30 TC 21 Z9 21 U1 1 U2 17 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP 6109 EP 6114 DI 10.1073/pnas.1218206110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125KE UT WOS:000317537900073 PM 23536305 ER PT J AU Lui, JC Baron, J AF Lui, Julian C. Baron, Jeffrey TI Evidence that Igf2 down-regulation in postnatal tissues and up-regulation in malignancies is driven by transcription factor E2f3 SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE childhood growth; genetic program; growth deceleration; cell proliferation; body size ID GROWTH-FACTOR-II; HUMAN BLADDER-CANCER; WILMS-TUMOR; INTRAUTERINE GROWTH; GENE; SPECIFICITY; EXPRESSION; OVEREXPRESSION; PROSTATE; MOUSE AB Insulin-like growth factor 2 (IGF2) is an important fetal growth factor. Its expression is dramatically down-regulated in multiple organs after birth but is frequently up-regulated in cancers. The mechanisms that drive down-regulation of IGF2 in postnatal tissues or the up-regulation in malignancy are unclear. We found evidence that E2F transcription factor 3 (E2F3) drives these changes in expression. E2f3 mRNA expression, protein expression, and binding to the Igf2 promoter all decreased with age postnatally in multiple mouse organs. In late juvenile hepatocytes, restoration of high E2f3 expression restored high Igf2 expression, indicating a causal relationship, but this induction did not occur in fetal hepatocytes, which already have high E2f3 and Igf2 expression. Transient expression of E2f3 in both HEK293 cells and in late juvenile hepatocytes were able to activate reporter constructs containing the mouse Igf2 promoter P2, which includes consensus E2F-binding sites. In humans, microarray data revealed declines in E2F3 and IGF2 expression with age similar to the mouse. In addition, E2F3-overexpressing human prostate and bladder cancers showed increased IGF2 expression, and levels of E2F3 and IGF2 mRNA in these cancers were positively correlated. Taken together, the findings suggest that down-regulation of E2f3 with age helps drive the dramatic decline in Igf2 expression in postnatal organs, and E2F3 overexpression in human cancers induces IGF2 overexpression. C1 [Lui, Julian C.; Baron, Jeffrey] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Growth & Dev, Program Dev Endocrinol & Genet, NIH, Bethesda, MD 20892 USA. RP Lui, JC (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Growth & Dev, Program Dev Endocrinol & Genet, NIH, Bethesda, MD 20892 USA. EM luichunk@mail.nih.gov RI Lui, Chun Kin Julian/E-2253-2012 FU Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health; Human Development, National Institutes of Health FX We thank Dr. Ivan Ovcharenko at the National Center for Biotechnology Information for help with the use of DiRE. This work was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health. NR 41 TC 17 Z9 17 U1 0 U2 11 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP 6181 EP 6186 DI 10.1073/pnas.1219079110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 125KE UT WOS:000317537900085 PM 23530192 ER PT J AU Smith, BM Kawut, SM Bluemke, DA Basner, RC Gomes, AS Hoffman, E Kalhan, R Lima, JAC Liu, CY Michos, ED Prince, MR Rabbani, L Rabinowitz, D Shimbo, D Shea, S Barr, RG AF Smith, Benjamin M. Kawut, Steven M. Bluemke, David A. Basner, Robert C. Gomes, Antoinette S. Hoffman, Eric Kalhan, Ravi Lima, Joao A. C. Liu, Chia-Ying Michos, Erin D. Prince, Martin R. Rabbani, LeRoy Rabinowitz, Daniel Shimbo, Daichi Shea, Steven Barr, R. Graham TI Pulmonary Hyperinflation and Left Ventricular Mass The Multi-Ethnic Study of Atherosclerosis COPD Study SO CIRCULATION LA English DT Article DE left ventricular mass; hyperinflation; chronic obstructive pulmonary disease ID OBSTRUCTIVE LUNG-DISEASE; HEART-FAILURE; CARDIOVASCULAR-DISEASE; RESPIRATORY MECHANICS; AIRWAYS OBSTRUCTION; CARDIAC STRUCTURE; FLOW LIMITATION; COR-PULMONALE; SLEEP-APNEA; HYPERTROPHY AB Background-Left ventricular (LV) mass is an important predictor of heart failure and cardiovascular mortality, yet determinants of LV mass are incompletely understood. Pulmonary hyperinflation in chronic obstructive pulmonary disease (COPD) may contribute to changes in intrathoracic pressure that increase LV wall stress. We therefore hypothesized that residual lung volume in COPD would be associated with greater LV mass. Methods and Results-The Multi-Ethnic Study of Atherosclerosis (MESA) COPD Study recruited smokers 50 to 79 years of age who were free of clinical cardiovascular disease. LV mass was measured by cardiac magnetic resonance. Pulmonary function testing was performed according to guidelines. Regression models were used to adjust for age, sex, body size, blood pressure, and other cardiac risk factors. Among 119 MESA COPD Study participants, the mean age was 69 +/- 6 years, 55% were male, and 65% had COPD, mostly of mild or moderate severity. Mean LV mass was 128 +/- 34 g. Residual lung volume was independently associated with greater LV mass (7.2 g per 1-SD increase in residual volume; 95% confidence interval, 2.2-12; P=0.004) and was similar in magnitude to that of systolic blood pressure (7.6 g per 1-SD increase in systolic blood pressure; 95% confidence interval, 4.3-11; P<0.001). Similar results were observed for the ratio of LV mass to end-diastolic volume (P=0.02) and with hyperinflation measured as residual volume to total lung capacity ratio (P=0.009). Conclusions-Pulmonary hyperinflation, as measured by residual lung volume or residual lung volume to total lung capacity ratio, is associated with greater LV mass. (Circulation. 2013;127:1503-1511.) C1 [Smith, Benjamin M.; Basner, Robert C.; Rabbani, LeRoy; Shimbo, Daichi; Shea, Steven; Barr, R. Graham] Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10032 USA. [Prince, Martin R.] Columbia Univ, Coll Phys & Surg, Dept Radiol, New York, NY 10032 USA. [Smith, Benjamin M.] McGill Univ, Dept Med, Ctr Hlth, Montreal, PQ, Canada. [Kawut, Steven M.] Univ Penn, Dept Med, Perelman Sch Med, Philadelphia, PA 19104 USA. [Bluemke, David A.] NIH, Dept Radiol & Imaging Sci, Bethesda, MD 20892 USA. [Gomes, Antoinette S.] David Geffen UCLA Sch Med, Los Angeles, CA USA. [Hoffman, Eric] Univ Iowa, Dept Radiol, Carver Coll Med, Iowa City, IA 52242 USA. [Kalhan, Ravi] Northwestern Univ, Asthma & COPD Program, Div Pulm & Crit Care, Feinberg Sch Med, Chicago, IL 60611 USA. [Lima, Joao A. C.; Michos, Erin D.] Johns Hopkins Univ, Dept Med, Baltimore, MD USA. [Liu, Chia-Ying] Johns Hopkins Univ, Dept Radiol, Baltimore, MD USA. [Rabinowitz, Daniel] Columbia Univ, Dept Stat, Mailman Sch Publ Hlth, New York, NY 10032 USA. [Shea, Steven; Barr, R. Graham] Columbia Univ, Dept Epidemiol, Mailman Sch Publ Hlth, New York, NY 10032 USA. RP Barr, RG (reprint author), Columbia Univ, Med Ctr, Presbyterian Hosp 9 E Room 105,630 W 168th St, New York, NY 10032 USA. EM rgb9@columbia.edu RI sebastianovitsch, stepan/G-8507-2013; Prince, Martin/S-6850-2016; OI Kalhan, Ravi/0000-0003-2443-0876; Bluemke, David/0000-0002-8323-8086; Prince, Martin/0000-0002-9883-0584 FU National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) [R01-HL093081, R01-HL077612, R01-HL075476, N01-HC95159-HC95169, K24-HL103844, UL1-TR000040, UL1-RR024156]; Fonds de la recherche en sante Quebec; ALS Association; Muscular Dystrophy Association; Will Rogers Respiratory Institute; Alpha-1 Foundation; American Lung Association; Roche Pharmaceuticals; Boehringer Ingelheim; Forest laboratories; Elevation Pharmaceuticals; American Cancer Society; Health Resources and Services Administration; National Center for Minority Health and Health Disparities; US Environmental Protection Agency; Cenestra Health FX This study was supported by National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) grants R01-HL093081, R01-HL077612, R01-HL075476, N01-HC95159-HC95169, K24-HL103844, UL1-TR000040, and UL1-RR024156, as well as Fonds de la recherche en sante Quebec.; Dr Basner has received research grants from the ALS Association, Muscular Dystrophy Association, NIH/NHLBI, and Will Rogers Respiratory Institute, as well as honoraria from UpToDate. Dr Hoffman has received research grants from the Alpha-1 Foundation, American Lung Association, Roche Pharmaceuticals, and NIH/NHLBI and has ownership interest in VIDA Diagnostics as a founder and shareholder. Dr Kalhan has received research grants from Boehringer Ingelheim, speakers' bureau compensation from Forest laboratories, and consultation compensation from Forest laboratories, Boehringer Ingelheim, and Elevation Pharmaceuticals. Dr Shea has received research grants from the American Cancer Society, Health Resources and Services Administration, National Center for Minority Health and Health Disparities, and NIH/NHLBI. Dr Barr has received research grants from the Alpha-1 Foundation, NIH/NHLBI, and US Environmental Protection Agency and research support from Cenestra Health. The other authors report no conflicts. NR 60 TC 23 Z9 25 U1 0 U2 6 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD APR 9 PY 2013 VL 127 IS 14 BP 1503 EP + DI 10.1161/CIRCULATIONAHA.113.001653 PG 15 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 122VS UT WOS:000317346900017 PM 23493320 ER PT J AU Vecchiarelli, AG Hwang, LC Mizuuchi, K AF Vecchiarelli, Anthony G. Hwang, Ling Chin Mizuuchi, Kiyoshi TI Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE bacterial chromosome segregation; ParA ATPase; plasmid segregation; spatial pattern organization; chromosome dynamics ID BACTERIAL CHROMOSOME SEGREGATION; ESCHERICHIA-COLI; DNA SEGREGATION; PATTERN-FORMATION; NONSPECIFIC DNA; P1 PARA; SOPA; PROTEIN; BINDING; ATPASE AB Increasingly diverse types of cargo are being found to be segregated and positioned by ParA-type ATPases. Several minimalistic systems described in bacteria are self-organizing and are known to affect the transport of plasmids, protein machineries, and chromosomal loci. One well-studied model is the F plasmid partition system, SopABC. In vivo, SopA ATPase forms dynamic patterns on the nucleoid in the presence of the ATPase stimulator, SopB, which binds to the sopC site on the plasmid, demarcating it as the cargo. To understand the relationship between nucleoid patterning and plasmid transport, we established a cell-free system to study plasmid partition reactions in a DNA-carpeted flowcell. We observed depletion zones of the partition ATPase on the DNA carpet surrounding partition complexes. The findings favor a diffusion-ratchet model for plasmid motion whereby partition complexes create an ATPase concentration gradient and then climb up this gradient toward higher concentrations of the ATPase. Here, we report on the dynamic properties of the Sop system on a DNA-carpet substrate, which further support the proposed diffusion-ratchet mechanism. C1 [Vecchiarelli, Anthony G.; Hwang, Ling Chin; Mizuuchi, Kiyoshi] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. RP Mizuuchi, K (reprint author), NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. EM kmizu@helix.nih.gov RI Hwang, Ling Chin/G-4078-2014 FU intramural research fund for the National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services; Nancy Nossal Fellowship FX We thank Vassili Ivanov for help with the microscope setup, Yong-Woon Han for helpful suggestions regarding the study, and Barbara Funnell for the P1 Par proteins. This work was supported by the intramural research fund for the National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services (K.M.). A.G.V. and L.C.H. were recipients of a Nancy Nossal Fellowship. NR 30 TC 36 Z9 36 U1 3 U2 12 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 9 PY 2013 VL 110 IS 15 BP E1390 EP E1397 DI 10.1073/pnas.1302745110 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130VK UT WOS:000317948000009 PM 23479605 ER PT J AU Prasad, V Rho, J Cifu, A AF Prasad, Vinay Rho, Jason Cifu, Adam TI The Inferior Vena Cava Filter How Could a Medical Device Be So Well Accepted Without Any Evidence of Efficacy? SO JAMA INTERNAL MEDICINE LA English DT Editorial Material ID ACUTE PULMONARY-EMBOLISM; VENOUS THROMBOEMBOLISM; FOLLOW-UP; PREVENTION; PREVALENCE; THROMBOSIS; INTERRUPTION; EXPERIENCE; MANAGEMENT C1 [Prasad, Vinay] NCI, Med Oncol Branch, NIH, Bethesda, MD 20892 USA. [Rho, Jason] Northwestern Univ, Dept Med, Chicago, IL 60611 USA. [Cifu, Adam] Univ Chicago, Dept Med, Chicago, IL 60637 USA. RP Prasad, V (reprint author), NCI, Med Oncol Branch, NIH, 10 Ctr Dr,10-12N226, Bethesda, MD 20892 USA. EM vinayak.prasad@nih.gov NR 19 TC 26 Z9 26 U1 0 U2 5 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-6106 J9 JAMA INTERN MED JI JAMA Intern. Med. PD APR 8 PY 2013 VL 173 IS 7 BP 493 EP 495 DI 10.1001/jamainternmed.2013.2725 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA 154NR UT WOS:000319683400003 PM 23552611 ER PT J AU Maggio, M Corsonello, A Ceda, GP Cattabiani, C Lauretani, F Butto, V Ferrucci, L Bandinelli, S Abbatecola, AM Spazzafumo, L Lattanzio, F AF Maggio, Marcello Corsonello, Andrea Ceda, Gian Paolo Cattabiani, Chiara Lauretani, Fulvio Butto, Valeria Ferrucci, Luigi Bandinelli, Stefania Abbatecola, Angela Marie Spazzafumo, Liana Lattanzio, Fabrizia TI Proton Pump Inhibitors and Risk of 1-Year Mortality and Rehospitalization in Older Patients Discharged From Acute Care Hospitals SO JAMA INTERNAL MEDICINE LA English DT Article ID NUTRITIONAL ASSESSMENT MNA; THERAPY; CLOPIDOGREL; VALIDATION; ILLNESS; PEOPLE; BURDEN; COHORT; SCALE; STATE AB Importance: The use of proton pump inhibitors (PPIs) has rapidly increased during the past several years. However, concern remains about risks associated with their long-term use in older populations. Objective: To investigate the relationship between the use of PPIs and the risk of death or the combined end point of death or rehospitalization in older patients discharged from acute care hospitals. Design: We investigated the relationship between PPI use and study outcomes using time-dependent Cox proportional hazards regression in patients 65 years or older discharged from acute care medical wards from April 1 to June 30, 2007. Setting: Eleven acute care medical wards. Participants: Four hundred ninety-one patients (mean [SD] age, 80.0 [5.9] years). Main Outcome Measures: Mortality and the combined end point of death or rehospitalization. Results: The use of PPIs was independently associated with mortality (hazard ratio, 1.51 [95% CI, 1.03-2.77]) but not with the combined end point (1.49 [0.98-2.17]). An increased risk of mortality was observed among patients exposed to high-dose PPIs vs none (hazard ratio, 2.59 [95% CI, 1.22-7.16]). Conclusions and Relevance: In older patients discharged from acute care hospitals, the use of high-dose PPIs is associated with increased 1-year mortality. Randomized controlled studies including older frail patients are needed. In the meantime, physicians need to use caution and balance benefits and harms in long-term prescription of high-dose PPIs. C1 [Maggio, Marcello; Ceda, Gian Paolo; Cattabiani, Chiara; Lauretani, Fulvio] Univ Hosp Parma, Dept Geriatr Rehabil, Parma, Italy. [Maggio, Marcello; Ceda, Gian Paolo; Cattabiani, Chiara; Butto, Valeria] Univ Parma, Dept Clin & Expt Med, Sect Geriatr, I-43100 Parma, Italy. [Corsonello, Andrea] INRCA, Unit Geriatr Pharmacoepidemiol, Cosenza, Italy. [Ferrucci, Luigi] Natl Inst Aging, Baltimore, MD USA. [Bandinelli, Stefania] Local Hlth Agcy 10, Geriatr Unit, Florence, Italy. [Abbatecola, Angela Marie; Spazzafumo, Liana; Lattanzio, Fabrizia] INRCA Ancona, Sci Direct, Ancona, Italy. RP Maggio, M (reprint author), Dept Clin & Expt Med, Sect Geriatr, Via Gramsci 14, I-43100 Parma, Italy. EM marcellomaggio2001@yahoo.it RI Lauretani, Fulvio/K-5115-2016; OI Lauretani, Fulvio/0000-0002-5287-9972; Ceda, Gian Paolo/0000-0002-9648-8295 FU Italian Ministry of Health [RF-INR-2005-127640]; Italian Ministry of Health and Emilia Romagna Region [RF-2010-2312659] FX This study was supported in part by grant RF-INR-2005-127640 from the Italian Ministry of Health (Pharmacosurveillance in the Elderly Care study) and by grant RF-2010-2312659 from the Italian Ministry of Health and Emilia Romagna Region. NR 34 TC 36 Z9 39 U1 1 U2 4 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-6106 J9 JAMA INTERN MED JI JAMA Intern. Med. PD APR 8 PY 2013 VL 173 IS 7 BP 518 EP 523 DI 10.1001/jamainternmed.2013.2851 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA 154NR UT WOS:000319683400008 PM 23460307 ER PT J AU Pardo, CA Buckley, A Thurm, A Lee, LC Azhagiri, A Neville, DM Swedo, SE AF Pardo, Carlos A. Buckley, Ashura Thurm, Audrey Lee, Li-Ching Azhagiri, Arun Neville, David M. Swedo, Susan E. TI A pilot open-label trial of minocycline in patients with autism and regressive features SO JOURNAL OF NEURODEVELOPMENTAL DISORDERS LA English DT Article DE Autism; Minocycline; Microglia; Neuroinflammation; Clinical trial; Cytokines; Chemokines; Metalloproteinases; Neurotrophins; BDNF ID AMYOTROPHIC-LATERAL-SCLEROSIS; PREFRONTAL CORTEX; MOUSE MODEL; MICROGLIA; BRAIN; DISORDERS; DISEASE; NEUROPROTECTION; SPECTRUM; COMMUNICATION AB Background: Minocycline is a tetracycline derivative that readily crosses the blood brain barrier and appears to have beneficial effects on neuroinflammation, microglial activation and neuroprotection in a variety of neurological disorders. Both microglial activation and neuroinflammation have been reported to be associated with autism. The study was designed to evaluate the effects of minocycline treatment on markers of neuroinflammation and autism symptomatology in children with autism and a history of developmental regression. Methods: Eleven children were enrolled in an open-label trial of six months of minocycline (1.4 mg/kg). Ten children completed the trial. Behavioral measures were collected and cerebrospinal fluid (CSF), serum and plasma were obtained before and at the end of minocycline treatment and were analyzed for markers of neuroinflammation. Results: Clinical improvements were negligible. The laboratory assays demonstrated significant changes in the expression profile of the truncated form of brain derived neurotrophic factor (BDNF) (P = 0.042) and hepatic growth factor (HGF) (P = 0.028) in CSF. In serum, the ratio of the truncated BDNF form and alpha-2 macroglobulin (alpha-2 M), was also significantly lower (P = 0.028) while the mature BDNF/alpha-2 M ratio revealed no difference following treatment. Only the chemokine CXCL8 (IL-8) was significantly different (P = 0.047) in serum while no significant changes were observed in CSF or serum in chemokines such as CCL2 (MCP-1) or cytokines such as TNF-alpha, CD40L, IL-6, IFN-gamma and IL-1 beta when pre- and post-treatment levels of these proteins were compared. No significant pre-and post-treatment changes were seen in the profiles of plasma metalloproteinases, putative targets of the effects of minocycline. Conclusions: Changes in the pre-and post-treatment profiles of BDNF in CSF and blood, HGF in CSF and CXCL8 (IL-8) in serum, suggest that minocycline may have effects in the CNS by modulating the production of neurotrophic growth factors. However, in this small group of children, no clinical improvements were observed during or after the six months of minocycline administration. C1 [Pardo, Carlos A.; Azhagiri, Arun] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21287 USA. [Buckley, Ashura; Thurm, Audrey; Neville, David M.; Swedo, Susan E.] NIMH, Pediat & Dev Neurosci Branch, Bethesda, MD 20892 USA. [Lee, Li-Ching] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21205 USA. RP Pardo, CA (reprint author), Johns Hopkins Univ, Sch Med, Dept Neurol, 600 North Wolfe St, Baltimore, MD 21287 USA. EM cpardov1@jhmi.edu FU National Institute of Mental Health; NIH [HHSN271200700179]; Bart McLean Fund for Neuroimmunology Research; Peter Emch Fund for Autism Research FX The research was funded by the Intramural Research Program of the National Institute of Mental Health. Dr. Pardo's laboratory received support from NIH contract HHSN271200700179, The Bart McLean Fund for Neuroimmunology Research and The Peter Emch Fund for Autism Research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Mental Health or the National Institutes of Health. NR 50 TC 11 Z9 11 U1 1 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1866-1947 J9 J NEURODEV DISORD JI J. Neurodev. Disord. PD APR 8 PY 2013 VL 5 AR 9 DI 10.1186/1866-1955-5-9 PG 9 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA 151ML UT WOS:000319464500001 PM 23566357 ER PT J AU Chu, IM Lai, WC Aprelikova, O El Touny, LH Kouros-Mehr, H Green, JE AF Chu, Isabel M. Lai, Wei-Chu Aprelikova, Olga El Touny, Lara H. Kouros-Mehr, Hosein Green, Jeffrey E. TI Expression of GATA3 in MDA-MB-231 Triple-negative Breast Cancer Cells Induces a Growth Inhibitory Response to TGF beta SO PLOS ONE LA English DT Article ID EPITHELIAL-MESENCHYMAL TRANSITION; E-CADHERIN; MAMMARY-GLAND; METASTASIS; DIFFERENTIATION; PROLIFERATION; TUMORIGENESIS; PROGRESSION; MIGRATION; LINES AB Transforming growth factor (beta 1TGF beta 1) can promote proliferation in late stage cancers but acts as a tumor suppressor in normal epithelial cells and in early stage cancers. Although, the TGF beta pathway has been shown to play a key role in tumorigenesis and metastasis, only a limited number of models have been developed to understand this process. Here, we present a novel model system to discern this paradoxical role of TGF beta 1 using the MDA-MB-231 (MB-231) cell line. The MB-231 triple-negative breast cancer cell line has been extensively characterized and has been shown to continue to proliferate and undergo epithelial-to-mesenchymal transition (EMT) upon TGF beta 1 stimulation. We have previously shown by microarray analysis that expression of GATA3 in MB-231 cells results in reprogramming of these cells from a basal to a luminal subtype associated with a reduction of metastasis and tumorigenesis when implanted as xenografts. We now demonstrate that GATA3 overexpression in these cells results in a reduction of TGF beta 1 response, reversal of EMT, and most importantly, restoration of sensitivity to the inhibitory effects on proliferation of TGF beta 1. Microarray analysis revealed that TGF beta 1 treatment resulted in reduction of several cell cycle effectors in 231-GATA3 cells but not in control cells. Furthermore, our microarray analysis revealed a significant increase of BMP5 in 231-GATA3 cells. We demonstrate that combined treatment of MB-231 control cells with TGF beta 1 and BMP5 results in a significant reduction of cellular proliferation. Thus, this model offers a means to further investigate potentially novel mechanisms involved in the switch in response to TGF beta 1 from tumor promoter to tumor suppressor through the reprogramming of a triple-negative breast cancer cell line by the GATA3 transcription factor. C1 [Chu, Isabel M.; Lai, Wei-Chu; Aprelikova, Olga; El Touny, Lara H.; Kouros-Mehr, Hosein; Green, Jeffrey E.] NCI, Transgen Oncogenesis & Genom Sect, Lab Canc Biol & Genet, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. RP Green, JE (reprint author), NCI, Transgen Oncogenesis & Genom Sect, Lab Canc Biol & Genet, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. EM jegreen@nih.gov FU Intramural program of the National Cancer Institute FX This work was supported by the Intramural program of the National Cancer Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 9 Z9 9 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2013 VL 8 IS 4 AR e61125 DI 10.1371/journal.pone.0061125 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130FG UT WOS:000317898000102 PM 23577196 ER PT J AU Graham, BS Enama, ME Nason, MC Gordon, IJ Peel, SA Ledgerwood, JE Plummer, SA Mascola, JR Bailer, RT Roederer, M Koup, RA Nabel, GJ AF Graham, Barney S. Enama, Mary E. Nason, Martha C. Gordon, Ingelise J. Peel, Sheila A. Ledgerwood, Julie E. Plummer, Sarah A. Mascola, John R. Bailer, Robert T. Roederer, Mario Koup, Richard A. Nabel, Gary J. CA VRC 008 Study Team TI DNA Vaccine Delivered by a Needle-Free Injection Device Improves Potency of Priming for Antibody and CD8+T-Cell Responses after rAd5 Boost in a Randomized Clinical Trial SO PLOS ONE LA English DT Article ID HEPATITIS-A VACCINE; T-CELL RESPONSES; NEUTRALIZING ANTIBODY; CANDIDATE VACCINE; HEALTHY-ADULTS; IMMUNOGENICITY EVALUATION; PHASE-1 SAFETY; JET INJECTION; PLASMID DNA; HIV-1 AB Background: DNA vaccine immunogenicity has been limited by inefficient delivery. Needle-free delivery of DNA using a CO2-powered Biojector (R) device was compared to delivery by needle and syringe and evaluated for safety and immunogenicity. Methods: Forty adults, 18-50 years, were randomly assigned to intramuscular (IM) vaccinations with DNA vaccine, VRC-HIVDNA016-00-VP, (weeks 0, 4, 8) by Biojector (R) 2000 (TM) or needle and syringe (N/S) and boosted IM at week 24 with VRC-HIVADV014-00-VP (rAd5) with N/S at 10(10) or 10(11) particle units (PU). Equal numbers per assigned schedule had low (<= 500) or high (>500) reciprocal titers of preexisting Ad5 neutralizing antibody. Results: 120 DNA and 39 rAd5 injections were given; 36 subjects completed follow-up research sample collections. IFN-gamma ELISpot response rates were 17/19 (89%) for Biojector (R) and 13/17 (76%) for N/S delivery at Week 28 (4 weeks post rAd5 boost). The magnitude of ELISpot response was about 3-fold higher in Biojector (R) compared to N/S groups. Similar effects on response rates and magnitude were observed for CD8+, but not CD4+ T-cell responses by ICS. Env-specific antibody responses were about 10-fold higher in Biojector-primed subjects. Conclusions: DNA vaccination by Biojector (R) was well-tolerated and compared to needle injection, primed for greater IFN-gamma ELISpot, CD8+ T-cell, and antibody responses after rAd5 boosting. Trial Registration: ClinicalTrials.gov NCT00109629 C1 [Graham, Barney S.; Enama, Mary E.; Gordon, Ingelise J.; Ledgerwood, Julie E.; Plummer, Sarah A.; Mascola, John R.; Bailer, Robert T.; Roederer, Mario; Koup, Richard A.; Nabel, Gary J.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. [Nason, Martha C.] NIAID, Biostat Res Branch, Div Clin Res, NIH, Bethesda, MD 20892 USA. [Peel, Sheila A.] Walter Reed Army Inst Res, US Mil HIV Res Program, Silver Spring, MD USA. RP Graham, BS (reprint author), NIAID, Vaccine Res Ctr, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM bgraham@nih.gov FU National Institute of Allergy and Infectious Diseases (NIAID) FX Funding for the conduct of this clinical trial was provided by the National Institute of Allergy and Infectious Diseases (NIAID) intramural research program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 34 TC 20 Z9 21 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2013 VL 8 IS 4 AR e59340 DI 10.1371/journal.pone.0059340 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130FG UT WOS:000317898000011 PM 23577062 ER PT J AU Lindskog, C Ellstrom, P Olsen, B Ponten, F van Riel, D Munster, VJ Gonzalez-Acuna, D Kuiken, T Jourdain, E AF Lindskog, Cecilia Ellstrom, Patrik Olsen, Bjorn Ponten, Fredrik van Riel, Debby Munster, Vincent J. Gonzalez-Acuna, Daniel Kuiken, Thijs Jourdain, Elsa TI European H16N3 Gull Influenza Virus Attaches to the Human Respiratory Tract and Eye SO PLOS ONE LA English DT Article ID A VIRUS; HEMAGGLUTININ SUBTYPE; RECEPTOR-BINDING; MALLARD DUCKS; WILD BIRDS; REPLICATION; PATTERNS; MAMMALS; HOST AB We explored the attachment of an H16N3 influenza virus to human, mallard, and gull tissues using virus histochemistry applied to tissue microarrays and employing human and mallard viruses as references. Of the viruses tested, the H16N3 gull virus most readily attached to the human respiratory tract and eye. These results underscore the need to assess the potential for gull influenza viruses to replicate in human tissues and further investigate the role of gulls in influenza virus ecology. C1 [Lindskog, Cecilia; Ponten, Fredrik] Uppsala Univ, Sci Life Lab, Dept Immunol Genet & Pathol, Uppsala, Sweden. [Ellstrom, Patrik; Olsen, Bjorn] Uppsala Univ, Dept Med Sci, Uppsala, Sweden. [van Riel, Debby; Kuiken, Thijs] Erasmus MC, Rotterdam, Netherlands. [Munster, Vincent J.] NIAID, Virol Lab, Div Intramural Res, NIH, Hamilton, MT USA. [Gonzalez-Acuna, Daniel] Univ Concepcion, Chillan, Chile. [Jourdain, Elsa] INRA, UR346, F-63122 St Genes Champanelle, France. RP Jourdain, E (reprint author), INRA, UR346, F-63122 St Genes Champanelle, France. EM elsa.jourdain@clermont.inra.fr OI Olsen, Bjorn/0000-0002-4646-691X; Munster, Vincent/0000-0002-2288-3196 FU Swedish Research Council for Spatial Planning and Agriculture [FORMAS 216-2009-1220]; Swedish Research Council Vetenskapsradet [VR 2010-36963-78952-78]; Knut and Alice Wallenberg Foundation; FONDECYT [1070464]; Division of Intramural Research, the National Institute of Allergy and Infectious Diseases; National Institutes of Health FX This study was financially supported by the Swedish Research Council for Spatial Planning and Agriculture (FORMAS 216-2009-1220), the Swedish Research Council Vetenskapsradet (VR 2010-36963-78952-78), the Knut and Alice Wallenberg Foundation, and the FONDECYT project (1070464). VJM is supported by the Division of Intramural Research, the National Institute of Allergy and Infectious Diseases, and the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 27 TC 2 Z9 2 U1 1 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2013 VL 8 IS 4 AR e60757 DI 10.1371/journal.pone.0060757 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130FG UT WOS:000317898000061 PM 23593303 ER PT J AU Vazquez-Prokopec, GM Bisanzio, D Stoddard, ST Paz-Soldan, V Morrison, AC Elder, JP Ramirez-Paredes, J Halsey, ES Kochel, TJ Scott, TW Kitron, U AF Vazquez-Prokopec, Gonzalo M. Bisanzio, Donal Stoddard, Steven T. Paz-Soldan, Valerie Morrison, Amy C. Elder, John P. Ramirez-Paredes, Jhon Halsey, Eric S. Kochel, Tadeusz J. Scott, Thomas W. Kitron, Uriel TI Using GPS Technology to Quantify Human Mobility, Dynamic Contacts and Infectious Disease Dynamics in a Resource-Poor Urban Environment SO PLOS ONE LA English DT Article ID HUMAN MOVEMENT; DEVELOPING-COUNTRIES; PANDEMIC INFLUENZA; UNITED-STATES; DENGUE VIRUS; NETWORKS; PATTERNS; TRANSMISSION; CHALLENGES; STRATEGIES AB Empiric quantification of human mobility patterns is paramount for better urban planning, understanding social network structure and responding to infectious disease threats, especially in light of rapid growth in urbanization and globalization. This need is of particular relevance for developing countries, since they host the majority of the global urban population and are disproportionally affected by the burden of disease. We used Global Positioning System (GPS) data-loggers to track the fine-scale (within city) mobility patterns of 582 residents from two neighborhoods from the city of Iquitos, Peru. We used similar to 2.3 million GPS data-points to quantify age-specific mobility parameters and dynamic co-location networks among all tracked individuals. Geographic space significantly affected human mobility, giving rise to highly local mobility kernels. Most (similar to 80%) movements occurred within 1 km of an individual's home. Potential hourly contacts among individuals were highly irregular and temporally unstructured. Only up to 38% of the tracked participants showed a regular and predictable mobility routine, a sharp contrast to the situation in the developed world. As a case study, we quantified the impact of spatially and temporally unstructured routines on the dynamics of transmission of an influenza-like pathogen within an Iquitos neighborhood. Temporally unstructured daily routines (e.g., not dominated by a single location, such as a workplace, where an individual repeatedly spent significant amount of time) increased an epidemic's final size and effective reproduction number by 20% in comparison to scenarios modeling temporally structured contacts. Our findings provide a mechanistic description of the basic rules that shape human mobility within a resource-poor urban center, and contribute to the understanding of the role of fine-scale patterns of individual movement and co-location in infectious disease dynamics. More generally, this study emphasizes the need for careful consideration of human social interactions when designing infectious disease mitigation strategies, particularly within resource-poor urban environments. C1 [Vazquez-Prokopec, Gonzalo M.; Bisanzio, Donal; Kitron, Uriel] Emory Univ, Dept Environm Studies, Atlanta, GA 30322 USA. [Vazquez-Prokopec, Gonzalo M.; Stoddard, Steven T.; Scott, Thomas W.; Kitron, Uriel] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Stoddard, Steven T.; Morrison, Amy C.; Scott, Thomas W.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Paz-Soldan, Valerie] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Global Hlth Syst & Dev, New Orleans, LA USA. [Elder, John P.] San Diego State Univ, Grad Sch Publ Hlth, San Diego, CA 92182 USA. [Ramirez-Paredes, Jhon; Halsey, Eric S.] US Naval Med Res Unit, Lima, Peru. [Ramirez-Paredes, Jhon; Halsey, Eric S.] US Naval Med Res Unit, Iquitos, Peru. [Kochel, Tadeusz J.] USN, Med Res Ctr, Silver Spring, MD USA. RP Vazquez-Prokopec, GM (reprint author), Emory Univ, Dept Environm Studies, Atlanta, GA 30322 USA. EM gmvazqu@emory.edu OI Bisanzio, Donal/0000-0002-7832-2291 FU U.S. National Institutes of Health - National Institute of Allergy and Infectious Diseases (NIH/NIAID) [R01 AI069341-01] FX Development of the ideas presented here was assisted by support from the Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate, U.S. Department of Homeland Security, and the Fogarty International Center, National Institutes of Health. This research was funded by a grant from the U.S. National Institutes of Health - National Institute of Allergy and Infectious Diseases (NIH/NIAID) award number R01 AI069341-01 (to TWS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 34 Z9 34 U1 1 U2 48 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2013 VL 8 IS 4 AR e58802 DI 10.1371/journal.pone.0058802 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 130FG UT WOS:000317898000005 PM 23577059 ER PT J AU Szu, SC Hunt, S Xie, GL Robbins, JB Schneerson, R Gupta, RK Zhao, ZG Tan, XM AF Szu, Shousun C. Hunt, Steven Xie, Guilin Robbins, John B. Schneerson, Rachel Gupta, Rajesh K. Zhao, Zhigang Tan, Xiaomei TI A human IgG anti-Vi reference for Salmonella typhi with weight-based antibody units assigned SO VACCINE LA English DT Article DE Typhoid; Capsule; Vi; Antibody; Reference; Serum; Standard ID STANDARD REFERENCE SERUM; CAPSULAR POLYSACCHARIDE; CONJUGATE VACCINES; FEVER; CHILDREN; EFFICACY; IMMUNIZATION; CARRIERS; VIETNAM; ACID AB Recent data showing the high incidence of typhoid fever in young children, the demonstration of safety and efficacy of a Vi conjugate for this age group, the safety and similar immunogenicity in infants when administrated concurrently with EPI vaccines, together with the interests of manufacturers and investigators in studying such conjugate vaccines prompted us to prepare a human IgG anti-Vi standard to facilitate this work. Volunteers were injected with an investigational Vi-recombinant Pseudomonas aeruginosa exoprotein A (Vi-rEPA) conjugate vaccine. Plasmas with the highest levels of IgG anti-Vi were pooled. The IgG anti-Vi content of this preparation, assayed by precipitin analysis with purified Vi, was 33 mu g/ml. Accordingly, the estimated IgG anti-Vi protective level of 3.5 ELISA unit/ml, derived from our efficacy trial of Vi-rEPA in 2-5 years old children, is equivalent to 4.3 mu g/ml. This reagent is suitable for comparison of immune response of Vi conjugate vaccines or for other purposes requiring anti-Vi measurement. Published by Elsevier Ltd. C1 [Szu, Shousun C.; Hunt, Steven; Robbins, John B.; Schneerson, Rachel] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD 20892 USA. [Xie, Guilin; Zhao, Zhigang; Tan, Xiaomei] Lanzhou Inst Biol Prod, Lanzhou, Gansu, Peoples R China. [Gupta, Rajesh K.] US FDA, Ctr Biol Evaluat & Res, Rockville, MD 20852 USA. RP Szu, SC (reprint author), NIH, Bldg 6,Room 1A06,9000 Rockville Pike, Bethesda, MD 20892 USA. EM szus@mail.nih.gov OI Hunt, Steven/0000-0003-3533-0627 FU National Institutes of Health, USA; Bill & Melinda Gates Foundation FX We thank Joni Trenbeath of Transfusion Transmitted Viruses Laboratory, Department of Transfusion Medicine, Clinical Center, NIH for molecular screening of the plasma, Dr. Xiao-mei Yang, Lanzhou Institute of Biologic Product, China for preparation of the plasma, Dr. James Kenney and Simleen Kaur of CBER, FDA, for formulation, filling and lyophilization of this preparation and Dr. Mark Miller of Fogarty International Center, NIH for help discussion. This work was supported by the National Institutes of Health, USA and a grant from the Bill & Melinda Gates Foundation. Contributions of Dr. Gupta to this study are an informal communication and represent his own best judgment. These comments do not bind or obligate FDA. NR 38 TC 8 Z9 10 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD APR 8 PY 2013 VL 31 IS 15 BP 1970 EP 1974 DI 10.1016/j.vaccine.2013.02.006 PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 125QY UT WOS:000317557600014 PM 23422143 ER PT J AU Zinselmeyer, BH Heydari, S Sacristan, C Nayak, D Cammer, M Herz, J Cheng, XX Davis, SJ Dustin, ML McGavern, DB AF Zinselmeyer, Bernd H. Heydari, Sara Sacristan, Catarina Nayak, Debasis Cammer, Michael Herz, Jasmin Cheng, Xiaoxiao Davis, Simon J. Dustin, Michael L. McGavern, Dorian B. TI PD-1 promotes immune exhaustion by inducing antiviral T cell motility paralysis SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID CHRONIC VIRAL-INFECTION; LYMPHOCYTIC CHORIOMENINGITIS VIRUS; HOST-PATHOGEN INTERACTIONS; IN-VIVO; REGULATORY T; STOP SIGNAL; IMMUNOLOGICAL SYNAPSES; DISEASE PROGRESSION; DENDRITIC CELLS; TRANSGENIC MICE AB Immune responses to persistent viral infections and cancer often fail because of intense regulation of antigen-specific T cells-a process referred to as immune exhaustion. The mechanisms that underlie the induction of exhaustion are not completely understood. To gain novel insights into this process, we simultaneously examined the dynamics of virus-specific CD8(+) and CD4(+) T cells in the living spleen by two-photon microscopy (TPM) during the establishment of an acute or persistent viral infection. We demonstrate that immune exhaustion during viral persistence maps anatomically to the splenic marginal zone/red pulp and is defined by prolonged motility paralysis of virus-specific CD8(+) and CD4(+) T cells. Unexpectedly, therapeutic blockade of PD-1-PD-L1 restored CD8(+) T cell motility within 30 min, despite the presence of high viral loads. This result was supported by planar bilayer data showing that PD-L1 localizes to the central supramolecular activation cluster, decreases antiviral CD8(+) T cell motility, and promotes stable immunological synapse formation. Restoration of T cell motility in vivo was followed by recovery of cell signaling and effector functions, which gave rise to a fatal disease mediated by IFN-gamma. We conclude that motility paralysis is a manifestation of immune exhaustion induced by PD-1 that prevents antiviral CD8(+) T cells from performing their effector functions and subjects them to prolonged states of negative immune regulation. C1 [Zinselmeyer, Bernd H.; Heydari, Sara; Nayak, Debasis; Herz, Jasmin; McGavern, Dorian B.] NINDS, NIH, Bethesda, MD 20892 USA. [Sacristan, Catarina; Cammer, Michael; Dustin, Michael L.] NYU, Sch Med, Dept Pathol, Mol Pathogenesis Program,Skirball Inst Biomol Med, New York, NY 10016 USA. [Cheng, Xiaoxiao; Davis, Simon J.] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX3 9DU, England. [Cheng, Xiaoxiao; Davis, Simon J.] Univ Oxford, John Radcliffe Hosp, MRC, Human Immunol Unit, Oxford OX3 9DU, England. RP McGavern, DB (reprint author), NINDS, NIH, Bethesda, MD 20892 USA. EM mcgavernd@mail.nih.gov OI McGavern, Dorian/0000-0001-9568-545X; Cammer, Michael/0000-0003-4930-1739; Dustin, Michael/0000-0003-4983-6389 FU National Institutes of Health (NIH) [P01AI080192]; Wellcome Trus; NIH FX This work was supported by National Institutes of Health (NIH) intramural program (D. B. McGavern) and grant P01AI080192 (M. L. Dustin, C. Sacristan, and M. Cammer), as well as by the Wellcome Trust (S.J. Davis). D. Nayak is presently supported by a NIH Intramural Competitive Fellowship. NR 71 TC 69 Z9 71 U1 0 U2 18 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD APR 8 PY 2013 VL 210 IS 4 BP 757 EP 774 DI 10.1084/jem.20121416 PG 18 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 121ZP UT WOS:000317284900011 PM 23530125 ER PT J AU Balasubramaniam, S Kayandan, S Pothayee, N Hu, N Lin, YN Pothayee, N Koretsky, AP House, MJ Woodward, RC St Pierre, TG Riffle, JS Davis, RM AF Balasubramaniam, Sharavanan Kayandan, Sanem Pothayee, Nipon Hu, Nan Lin, Yin-Nian Pothayee, Nikorn Koretsky, Alan P. House, Michael J. Woodward, Robert C. St Pierre, Timothy G. Riffle, Judy S. Davis, Richey M. TI Structure-relaxivity relationships of well-defined magnetite clusters for sensitive magnetic resonance imaging SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Balasubramaniam, Sharavanan; Kayandan, Sanem; Pothayee, Nipon; Riffle, Judy S.; Davis, Richey M.] Virginia Tech, Macromol & Interfaces Inst, Blacksburg, VA 24060 USA. [Kayandan, Sanem; Hu, Nan; Lin, Yin-Nian; Riffle, Judy S.] Virginia Tech, Dept Chem, Blacksburg, VA 24060 USA. [Davis, Richey M.] Virginia Tech, Dept Chem Engn, Blacksburg, VA 24060 USA. [Pothayee, Nikorn; Koretsky, Alan P.] NINDS, Lab Funct & Mol Imaging, NIH, Bethesda, MD 20892 USA. [House, Michael J.; Woodward, Robert C.; St Pierre, Timothy G.] Univ Western Australia, Sch Phys, Crawley, WA 6009, Australia. EM shravan1@vt.edu RI House, Michael/B-6477-2008; St Pierre, Timothy/A-5509-2008 OI House, Michael/0000-0002-1752-6274; St Pierre, Timothy/0000-0003-4682-4972 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 81-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303605181 ER PT J AU Barros, MDR Rein, A Datta, S Losche, M Nanda, H AF Barros, Marilia D. R. Rein, Alan Datta, Siddhartha Loesche, Mathias Nanda, Hirsh TI Membrane association and structure of retroviral assembly proteins SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Barros, Marilia D. R.; Loesche, Mathias; Nanda, Hirsh] Carnegie Mellon Univ, Dept Phys, Pittsburgh, PA 15213 USA. [Datta, Siddhartha; Loesche, Mathias; Nanda, Hirsh] NIST, NIST Ctr Neutron Res, Gaithersburg, MD 20899 USA. [Rein, Alan] NCI, HIV Drug Resistance Program, Frederick, MD 21702 USA. EM hirsh.nanda@nist.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 47-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603642 ER PT J AU Brown, N Buszek, KR AF Brown, Neil Buszek, Keith R. TI Regioselectivity of Diels-Alder reactions between 6,7-dehydrobenzofuran and 2-substituted furans SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Brown, Neil; Buszek, Keith R.] Univ Missouri, Dept Chem, Kansas City, MO 64110 USA. [Brown, Neil; Buszek, Keith R.] Univ Kansas, NIH, Ctr Excellence Chem Methodol & Lib Dev, Lawrence, KS 66047 USA. EM buszekk@umkc.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 926-ORGN PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603573 ER PT J AU Clements, GV Yepikhin, AS Bashoff, HI Dowd, CS AF Clements, Gail V. Yepikhin, Alex S. Bashoff, Helena I. Dowd, Cynthia S. TI Mycobacterium tuberculosis proteasome inhibitors SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Clements, Gail V.; Yepikhin, Alex S.; Dowd, Cynthia S.] George Washington Univ, Dept Chem, Washington, DC 20052 USA. [Bashoff, Helena I.] NIH, Inst Allergy & Infect Dis, Bethesda, MD 20892 USA. EM gvclem@gwmail.gwu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 81-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602475 ER PT J AU Ferre-D'Amare, AR AF Ferre-D'Amare, Adrian R. TI Did the glmS ribozyme-riboswitch evolve from a coenzyme-independent self-cleaving RNA? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Ferre-D'Amare, Adrian R.] NHLBI, Biochem & Biophys Ctr, Bethesda, MD 20892 USA. EM adrian.ferre@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 135-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603729 ER PT J AU Gangjee, A Zhao, S Namjoshi, O Smith, CD Hamel, E AF Gangjee, Aleem Zhao, Sai Namjoshi, Ojas Smith, Charles D. Hamel, Ernest TI Design, synthesis, and biological evaluation of 7-benzyl-4-alkyl-5-substituted-7H-pyrrolo[2,3-d]pyrimidin-2-amines as potential antimitotic agents that also reverse tumor resistance SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Gangjee, Aleem; Zhao, Sai; Namjoshi, Ojas] Duquesne Univ, Dept Med Chem, Pittsburgh, PA 15282 USA. [Smith, Charles D.] Med Univ S Carolina, Dept Pharmaceut Sci, Charleston, SC 29425 USA. [Hamel, Ernest] NCI, Div Canc Treatment & Diag, Frederick, MD 21702 USA. EM zhaos2390@duq.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 304-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602324 ER PT J AU Guerard, F Lee, YS Tripier, R Szajek, LP Deschamps, JR Brechbiel, MW AF Guerard, Francois Lee, Yong-Sok Tripier, Raphael Szajek, Lawrence P. Deschamps, Jeffrey R. Brechbiel, Martin W. TI Complexation chemistry of Zr(IV) with hydroxamates: Crystallography, density functional theory, and potentiometry as new tools for the development of chelators for Zr-89 in nuclear medicine SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Guerard, Francois; Brechbiel, Martin W.] NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, Bethesda, MD 20892 USA. [Lee, Yong-Sok] NIH, Ctr Mol Modeling, Ctr Informat Technol, Bethesda, MD 20892 USA. [Tripier, Raphael] Univ Brest, CNRS, Lab Chim Electrochim Mol & Chim Analyt, UMR 6521, Brest, France. [Szajek, Lawrence P.] NIH, Positron Emiss Tomog Dept, Warren Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA. [Deschamps, Jeffrey R.] Naval Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. EM guerardf@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 1089-INOR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303600826 ER PT J AU Hall, MD AF Hall, Matthew D. TI Resistance is useless: Structure-activity and mechanistic studies of small molecules that induce collateral sensitivity in multidrug-resistant cancer cells SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Hall, Matthew D.] NCI, Cell Biol Lab, Bethesda, MD 20892 USA. EM hallma@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 208-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602220 ER PT J AU Horkay, F Basser, PJ Hecht, AM Geissler, E AF Horkay, Ferenc Basser, Peter J. Hecht, Anne-Marie Geissler, Erik TI Structure and interactions in hyaluronic acid solutions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Horkay, Ferenc; Basser, Peter J.] NICHD, Sect Tissue Biophys & Biomimet, NIH, Bethesda, MD 20892 USA. [Hecht, Anne-Marie; Geissler, Erik] Univ J Fourier Grenoble, Lab Interdisciplinaire Phys, CNRS, UMR 5588, St Martin Dheres, France. EM horkayf@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 518-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303604470 ER PT J AU Lyman, E Sandar, L Sodt, A Pastor, RW AF Lyman, Edward Sandar, Logan Sodt, Alex Pastor, Richard W. TI Modeling liquid-liquid phase separation and lipid transport on the Anton Supercomputer SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Lyman, Edward; Sandar, Logan] Univ Delaware, Newark, DE 19716 USA. [Sodt, Alex; Pastor, Richard W.] NHLBI, Lab Computat Biol, Bethesda, MD USA. EM elyman@udel.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 82-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603677 ER PT J AU Maina, LW Chandrasoma, N Buszek, KR AF Maina, Lincoln W. Chandrasoma, Nalin Buszek, Keith R. TI Novel polycyclic annulated indole scaffolds via indole aryne cycloaddition, Pd(0)-catalyzed cross-coupling, and ROM/RCM methodologies SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Maina, Lincoln W.; Chandrasoma, Nalin; Buszek, Keith R.] Univ Missouri, Dept Chem, Kansas City, MO 64110 USA. [Buszek, Keith R.] Univ Kansas, NIH, Ctr Excellence Chem Methodol & Lib Dev, Lawrence, KS 64110 USA. EM lwmfh3@mail.umkc.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 298-ORGN PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602971 ER PT J AU Minton, AP AF Minton, Allen P. TI Synergistic effects of an "inert" cosolute and biological substrates upon functionally related conformational equilibria in an enzyme and in a riboswitch SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Minton, Allen P.] NIDDK, NIH, Bethesda, MD 20892 USA. EM minton@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 212-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603804 ER PT J AU Miranda, KM Basudhar, D Cheng, RC Bharadwaj, G Ridnour, LA Wink, DA AF Miranda, Katrina M. Basudhar, Debashree Cheng, Robert C. Bharadwaj, Gaurav Ridnour, Lisa A. Wink, David A. TI Nitrogen oxide releasing NSAIDs as anticancer agents SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Miranda, Katrina M.; Basudhar, Debashree; Bharadwaj, Gaurav] Univ Arizona, Dept Chem & Biochem, Tucson, AZ 86721 USA. [Cheng, Robert C.; Ridnour, Lisa A.; Wink, David A.] NIH, Radiat Biol Branch, Canc Res Ctr, Bethesda, MD 20892 USA. EM kmiranda@email.arizona.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 45-INOR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303601510 ER PT J AU Pepe, A Ferroni, C Kim, YS Guerrini, A Trepel, JB Malhotra, SV Varchi, G AF Pepe, Antonella Ferroni, Claudia Kim, Yeong Sang Guerrini, Andrea Trepel, Jane B. Malhotra, Sanjay V. Varchi, Greta TI Synthesis and the biological evaluation of triazole-based novel antiandrogens SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Pepe, Antonella; Malhotra, Sanjay V.] Frederick Natl Lab Canc Res, Lab Synthet Chem SAIC, Frederick, MD 21702 USA. [Ferroni, Claudia; Guerrini, Andrea; Varchi, Greta] Inst Organ Synth & Photoreact ISOF, Consiglio Nazl Ric, Bologna, Italy. [Kim, Yeong Sang; Trepel, Jane B.] NCI, Med Oncol Branch, Ctr Canc Res, NIH,DHHS, Bethesda, MD 20892 USA. EM pepea@mail.nih.gov RI Ferroni, Claudia/A-4487-2014 OI Ferroni, Claudia/0000-0002-7386-1624 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 101-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602111 ER PT J AU Pickard, FC Simmonett, AC Brooks, BR AF Pickard, Frank C. Simmonett, Andrew C. Brooks, Bernard R. TI Efficient arbitrary order implementation of anisotropic electrostatic interactions in the CHARMM simulation package SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Pickard, Frank C.; Brooks, Bernard R.] NHLBI, Lab Computat Biol, Bethesda, MD 20892 USA. [Simmonett, Andrew C.] Univ Georgia, Ctr Computat Quantum Chem, Athens, GA 30602 USA. EM pickard81@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 255-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603846 ER PT J AU Planalp, RP Brechbiel, MW Torti, FM Torti, SV AF Planalp, Roy P. Brechbiel, Martin W. Torti, Frank M. Torti, Suzy V. TI Influence of ligand structure on Fe(II) spin-state and cytotoxicity of tripodal chelators SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Planalp, Roy P.] Univ New Hampshire, Dept Chem, Durham, NH 03824 USA. [Torti, Frank M.; Torti, Suzy V.] Univ Connecticut, Ctr Hlth, Dept Mol Microbial & Struct Biol, Farmington, CT 06030 USA. [Brechbiel, Martin W.] NCI, Radiat Oncol Branch, Bethesda, MD 20892 USA. EM roy.planalp@unh.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 95-INOR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602055 ER PT J AU Sari, O Metifiot, M Marchand, C Roy, V Pommier, Y Agrofoglio, LA AF Sari, Ozkan Metifiot, Mathieu Marchand, Christophe Roy, Vincent Pommier, Yves Agrofoglio, Luigi A. TI Synthesis of b-diketoacid dihydropyrimidine derivatives targeting HIV integrase SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Sari, Ozkan; Roy, Vincent; Agrofoglio, Luigi A.] Univ Orleans, CNRS, UMR 7311, ICOA, Orleans, France. [Metifiot, Mathieu; Marchand, Christophe; Pommier, Yves] NCI, Mol Pharmacol Lab, NIH, Bethesda, MD 20892 USA. EM ozkan.sari@univ-orleans.fr NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 404-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602423 ER PT J AU Soubias, O Teague, WE Gawrisch, K AF Soubias, Olivier Teague, Walter E., Jr. Gawrisch, Klaus TI Reconstitution of G protein-coupled membrane receptors into single tubular bilayers using nanoporous substrates SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Soubias, Olivier; Teague, Walter E., Jr.; Gawrisch, Klaus] NIAAA, LMBB, NIH, Bethesda, MD 20892 USA. EM gawrisch@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 128-PHYS PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303603722 ER PT J AU Wang, YL AF Wang, Yanli TI PubChem BioAssay: A public information resource for medicinal chemistry research SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Wang, Yanli] NIH, NLM, NCBI, Bethesda, MD 20894 USA. EM ywang@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 24-MEDI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303602254 ER PT J AU Wink, DA Switzer, CW Heincke, J Ridnour, LA Cheng, R Glynn, S Ambs, S AF Wink, David A. Switzer, Christopher W. Heincke, Julie Ridnour, Lisa A. Cheng, Robert Glynn, Sharon Ambs, Stefan TI Oncogenic properties of nitrosation in ERa(-) breast cancer SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Spring Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Wink, David A.; Switzer, Christopher W.; Heincke, Julie; Ridnour, Lisa A.; Cheng, Robert] NCI, Radiat Biol Branch, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. [Glynn, Sharon] NUI Galaway, Prostate Canc Inst, Galway, Ireland. [Ambs, Stefan] NCI, Human Carcinogenesis Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM wink@mail.nih.gov RI Glynn, Sharon/D-7136-2013 OI Glynn, Sharon/0000-0003-1459-2580 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 46-INOR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 216SF UT WOS:000324303601521 ER PT J AU Bolton, E AF Bolton, Evan TI PubChem3D: A virtual screening platform SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Bolton, Evan] NCBI, PubChem, NLM, NIH, Bethesda, MD 20892 USA. EM bolton@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 114-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303640 ER PT J AU Bolton, E AF Bolton, Evan TI Linking chemical biology information within PubChem SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Bolton, Evan] NIH, PubChem, NCBI, NLM, Bethesda, MD 20892 USA. EM bolton@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 83-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303609 ER PT J AU Bolton, E AF Bolton, Evan TI PubChem: A community driven resource SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Bolton, Evan] NIH, PubChem, NCBI, NLM, Bethesda, MD 20892 USA. EM bolton@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 77-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303603 ER PT J AU Bolton, E AF Bolton, Evan TI New ways to mine disparate screening data in PubChem SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Bolton, Evan] NCBI, PubChem, NLM, NIH, Bethesda, MD USA. EM bolton@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 2-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303530 ER PT J AU Davis, CD AF Davis, Cindy D. TI Bioactive dietary ingredients for cancer prevention SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Davis, Cindy D.] NIH, Off Dietary Supplements, Rockville, MD 20892 USA. EM davisci@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 116-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851300109 ER PT J AU Geer, LY Han, LY He, SQ Wang, YL Bolton, EE Bryant, SH AF Geer, Lewis Y. Han, Lianyi He, Siqian Wang, Yanli Bolton, Evan E. Bryant, Stephen H. TI PubChem DataDicer: A data warehouse for rapid querying of bioassay data SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Geer, Lewis Y.; Han, Lianyi; He, Siqian; Wang, Yanli; Bolton, Evan E.; Bryant, Stephen H.] NIH, NLM, NCBI, Bethesda, MD 20894 USA. EM lewisg@ncbi.nlm.nih.gov RI Geer, Lewis/H-2714-2014 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 3-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303531 ER PT J AU Ghysels, A Venable, RM Pastor, RW Hummer, G AF Ghysels, An Venable, Richard M. Pastor, Richard W. Hummer, Gerhard TI Oxygen diffusion in water, alkanes, and lipid bilayers SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Ghysels, An] Univ Ghent, Ctr Mol Modeling, B-9000 Ghent, Belgium. [Venable, Richard M.; Pastor, Richard W.] NHLBI, NIH, Bethesda, MD 20824 USA. [Hummer, Gerhard] NIDDK, NIH, Bethesda, MD 20824 USA. EM an.ghysels@ugent.be RI Ghysels, An/M-9095-2015; Hummer, Gerhard/A-2546-2013 OI Hummer, Gerhard/0000-0001-7768-746X NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 420-COMP PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851304423 ER PT J AU Guasch, L Sitzmann, M Nicklaus, MC AF Guasch, Laura Sitzmann, Markus Nicklaus, Marc C. TI Analysis of tautomerism in databases of commercially available compounds SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Guasch, Laura; Sitzmann, Markus; Nicklaus, Marc C.] NCI, Biol Chem Lab, Ctr Canc Res, Frederick Natl Lab Canc Res,NIH,DHHS, Frederick, MD 21702 USA. EM lguasch@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 104-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303630 ER PT J AU Guha, R Mathews, L Keller, J Shinn, P Thomas, C Simeonov, A Ferrar, M AF Guha, Rajarshi Mathews, Lesley Keller, John Shinn, Paul Thomas, Craig Simeonov, Anton Ferrar, Marc TI Characterization and visualization of compound combination responses in a high throughout setting SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Guha, Rajarshi; Mathews, Lesley; Keller, John; Shinn, Paul; Thomas, Craig; Simeonov, Anton; Ferrar, Marc] NIH Ctr Adv Translat Sci, Rockville, MD 20850 USA. EM guhar@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 34-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303562 ER PT J AU Guha, R Lahr, D Bittker, J Chung, TDY Southern, M Chatwin, S Yang, JJ Ursu, O Bologa, CG Oprea, TI Dawson, E Stauffer, SR Lindsley, CW Vempati, U Kucuk, H Schurer, SC Brudz, S Clemons, PA de Souza, A Southall, N Nguyen, DT Braisted, J Peryea, T AF Guha, Rajarshi Lahr, David Bittker, Joshua Chung, Thomas D. Y. Southern, Mark Chatwin, Simon Yang, Jeremy J. Ursu, Oleg Bologa, Christian G. Oprea, Tudor I. Dawson, Eric Stauffer, Shaun R. Lindsley, Craig W. Vempati, Uma Kucuk, Hande Schurer, Stephan C. Brudz, Stephen Clemons, Paul A. de Souza, Andrea Southall, Noel Dac-Trung Nguyen Braisted, John Peryea, Tyler TI BioAssay Research Database: A platform to support the collection, management, and analysis of chemical biology data SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Guha, Rajarshi; Southall, Noel; Dac-Trung Nguyen; Braisted, John; Peryea, Tyler] NIH, Ctr Adv Translat Sci, Rockville, MD 20850 USA. [Lahr, David; Bittker, Joshua; Chatwin, Simon; Brudz, Stephen; Clemons, Paul A.; de Souza, Andrea] Broad Inst, Cambridge, MA 02143 USA. [Chung, Thomas D. Y.] Sanford Burnham Med Res Inst, Conrad Prebys Ctr Chem Genom, La Jolla, CA 92037 USA. [Yang, Jeremy J.; Ursu, Oleg; Bologa, Christian G.; Oprea, Tudor I.] Univ New Mexico, Dept Internal Med, Albuquerque, NM 87131 USA. [Dawson, Eric; Stauffer, Shaun R.; Lindsley, Craig W.] Vanderbilt Univ, Nashville, TN 37232 USA. [Vempati, Uma; Kucuk, Hande; Schurer, Stephan C.] Univ Miami, Miami, FL 33101 USA. [Southern, Mark] Scripps Res Inst, Jupiter, FL 33458 USA. EM guhar@mail.nih.gov RI Oprea, Tudor/A-5746-2011 OI Oprea, Tudor/0000-0002-6195-6976 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 7-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303535 ER PT J AU Han, LY AF Han, Lianyi TI PubChem widgets SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Han, Lianyi] NIH, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. EM hanl@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 4-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303532 ER PT J AU Holland, RJ Maciag, AE Saavedra, JE Keefer, LK Chakrapani, H AF Holland, Ryan J. Maciag, Anna E. Saavedra, Joseph E. Keefer, Larry K. Chakrapani, Harinath TI Crosslinking glutathionylation, a novel thiol modification induced by compounds of the diazeniumdiolate class SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Holland, Ryan J.; Keefer, Larry K.] NCI, Ctr Canc Res, Biol Chem Lab, Frederick, MD 21702 USA. [Maciag, Anna E.; Saavedra, Joseph E.] SAIC Frederick, Basic Sci Program, Biol Chem Lab, Frederick, MD 21702 USA. [Chakrapani, Harinath] Indian Inst Sci Educ & Res, Pune 411008, Maharashtra, India. EM hollandrj@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 199-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851300794 ER PT J AU Horkay, F Horkayne-Szakaly, I Dimitriadis, EK Silva, C Basser, PJ AF Horkay, Ferenc Horkayne-Szakaly, Iren Dimitriadis, Emilios K. Silva, Candida Basser, Peter J. TI Morphology and load-bearing properties of cartilage matrix SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Horkay, Ferenc; Horkayne-Szakaly, Iren; Silva, Candida; Basser, Peter J.] NICHD, Sect Tissue Biophys & Biomimet, NIH, Bethesda, MD 20892 USA. [Dimitriadis, Emilios K.] NIBIB, Lab Bioengn & Phys Sci, NIH, Bethesda, MD 20892 USA. EM horkayf@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 338-BIOT PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851301167 ER PT J AU Hu, X Huang, ZH Southall, N Xiao, JB Chen, CZ Barnaeva, E Marugan, JJ Ferrer, M Zheng, W Agoulnik, IU Agoulnik, AI AF Hu, Xin Huang, Zaohua Southall, Noel Xiao, Jingbo Chen, Catherine Z. Barnaeva, Elena Marugan, Juan J. Ferrer, Marc Zheng, Wei Agoulnik, Irina U. Agoulnik, Alexander I. TI Structural modeling of the relaxin peptide receptor and its small molecule agonists SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Hu, Xin; Southall, Noel; Xiao, Jingbo; Chen, Catherine Z.; Barnaeva, Elena; Marugan, Juan J.; Ferrer, Marc; Zheng, Wei] NIH, Chem Genom Ctr 1NIH, Natl Ctr, Natl Ctr Adv Translat Sci, Rockville, MD 20850 USA. [Huang, Zaohua; Agoulnik, Alexander I.] Florida Int Univ, Herbert Wertheim Coll Med, Human & Mol Genet 2Dept, Miami, FL 33199 USA. [Agoulnik, Irina U.] Florida Int Univ, Herbert Wertheim Coll Med, Dept Cellular Biol & Pharmacol, Miami, FL 33199 USA. EM hux61@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 243-COMP PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851304270 ER PT J AU O'Boyle, NM Bolton, E Sayle, RA AF O'Boyle, Noel M. Bolton, Evan Sayle, Roger A. TI Roundtripping between small-molecule and biopolymer representations SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [O'Boyle, Noel M.; Sayle, Roger A.] NextMove Software, Cambridge, England. [Bolton, Evan] Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. EM noel@nextmovesoftware.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 86-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303612 ER PT J AU Peryea, T Braisted, J Jadhav, A Guha, R Southall, N Nguyen, T AF Peryea, Tyler Braisted, John Jadhav, Ajit Guha, Rajarshi Southall, Noel Dac-Trung Nguyen TI From hits to leads: Data visualization of chemical scaffolds beyond traditional SAR exploration SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Peryea, Tyler; Braisted, John; Jadhav, Ajit; Guha, Rajarshi; Southall, Noel; Dac-Trung Nguyen] Natl Ctr Adv Translat Sci, Div Preclin Innovat, Rockville, MD 20850 USA. EM tyler.peryea@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 36-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303564 ER PT J AU Ponraj, P Chen, WZ Gong, R Ying, TL Zhu, ZY Feng, Y Dimitrov, DS AF Ponraj, Prabakaran Chen, Weizao Gong, Rui Ying, Tianlei Zhu, Zhongyu Feng, Yang Dimitrov, Dimiter S. TI Engineered human antibody constant domains and fragments: Design, biophysical characterization, and applications SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Ponraj, Prabakaran; Chen, Weizao; Gong, Rui; Ying, Tianlei; Zhu, Zhongyu; Feng, Yang; Dimitrov, Dimiter S.] NIH, Prot Interact Grp, CCR Nanobiol Program, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA. [Ponraj, Prabakaran] Sci Applicat Int Corp Frederick Inc, Basic Res Program, Frederick, MD 21702 USA. EM prabakaran.ponraj@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 116-BIOT PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851300948 ER PT J AU Poroikov, VV Filimonov, DA Lagunin, AA Gloriozova, TA Tarasova, OA Pogodin, PV Nicklaus, MC AF Poroikov, Vladimir V. Filimonov, Dmitry A. Lagunin, Alexey A. Gloriozova, Tatyana A. Tarasova, Olga A. Pogodin, Pavel V. Nicklaus, Marc C. TI Virtual high-throughput screening of novel pharmacological agents based on PASS predictions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Poroikov, Vladimir V.; Filimonov, Dmitry A.; Lagunin, Alexey A.; Gloriozova, Tatyana A.; Tarasova, Olga A.; Pogodin, Pavel V.] Russian Acad Med Sci, Dept Bioinforat, Orekhovich Inst Biomed Chem, Moscow, Russia. [Poroikov, Vladimir V.; Pogodin, Pavel V.] Russian Natl Res Med Univ, Med Biol Fac, Moscow, Russia. [Nicklaus, Marc C.] NCI, Biol Chem Lab, NIH, Frederick, MD 21701 USA. EM vladimir.poroikov@ibmc.msk.ru RI Poroikov, Vladimir/O-2769-2013; Lagunin, Alexey/G-3745-2010; Pogodin, Pavel/F-1123-2017 OI Poroikov, Vladimir/0000-0001-7937-2621; Lagunin, Alexey/0000-0003-1757-8004; NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 116-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303642 ER PT J AU Shen, M Rosenthal, AS Mott, BT Tanega, C Auld, DS Austin, CP Maloney, DJ Thomas, CJ AF Shen, Min Rosenthal, Andrew S. Mott, Bryan T. Tanega, Cordelle Auld, Douglas S. Austin, Christopher P. Maloney, David J. Thomas, Craig J. TI Targeting the alternative gene splicing related proteins: Identification of potent and selective cdc2-like (Clk) kinase inhibitors SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Shen, Min; Rosenthal, Andrew S.; Mott, Bryan T.; Tanega, Cordelle; Austin, Christopher P.; Maloney, David J.; Thomas, Craig J.] NIH, Natl Ctr Adv Translat Sci, Rockville, MD 20850 USA. [Auld, Douglas S.] Novartis Inst Biomed Res, Cambridge, MA 02139 USA. EM shenmin@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 241-COMP PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851304268 ER PT J AU Sitzmann, M Weidlich, IE Filippov, IV Liao, CZ Peach, ML Ihlenfeldt, WD Karki, RG Borodina, YV Cachau, RE Nicklaus, MC AF Sitzmann, Markus Weidlich, Iwona E. Filippov, Igor V. Liao, Chenzhong Peach, Megan L. Ihlenfeldt, Wolf-Dietrich Karki, Rajeshri G. Borodina, Yulia V. Cachau, Raul E. Nicklaus, Marc C. TI PDB ligand conformational energies calculated quantum-mechanically SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Sitzmann, Markus; Weidlich, Iwona E.; Filippov, Igor V.; Liao, Chenzhong; Peach, Megan L.; Karki, Rajeshri G.; Nicklaus, Marc C.] NCI, CADD Grp, Ctr Canc Res, NIH, Frederick, MD 21702 USA. [Borodina, Yulia V.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. [Cachau, Raul E.] Frederick Natl Lab Canc Res, SAIC Frederick, Frederick, MD 21702 USA. [Ihlenfeldt, Wolf-Dietrich] Xemistry GmbH, Konigstein, Germany. EM mn1@helix.nih.gov NR 0 TC 0 Z9 0 U1 3 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 150-COMP PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851304201 ER PT J AU Sitzmann, M Zakharov, AV Pamies, LG Nicklaus, MC AF Sitzmann, Markus Zakharov, Alexey V. Pamies, Laura Guasch Nicklaus, Marc C. TI NCI/CADD chemical structure Web services SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Sitzmann, Markus; Zakharov, Alexey V.; Pamies, Laura Guasch; Nicklaus, Marc C.] NCI, Biol Chem Lab, Ctr Canc Res, Frederick Natl Lab Canc Res,NIH,DHHS, Frederick, MD 21702 USA. EM sitzmann@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 78-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303604 ER PT J AU Teague, WE Soubias, O Petrache, H Fuller, N Hines, KG Rand, RP Gawrisch, K AF Teague, Walter E., Jr. Soubias, Olivier Petrache, Horia Fuller, Nola Hines, Kirk G. Rand, R. Peter Gawrisch, Klaus TI Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Teague, Walter E., Jr.; Soubias, Olivier; Hines, Kirk G.; Gawrisch, Klaus] NIAAA, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD 20892 USA. [Petrache, Horia] Indiana Univ Perdue Univ, Dept Phys, Indianapolis, IN 46202 USA. [Fuller, Nola; Rand, R. Peter] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada. EM gawrisch@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 95-COLL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303749 ER PT J AU Wang, YL AF Wang, Yanli TI PubChem BioAssay: A public database for chemical biology data SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Wang, Yanli] NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. EM ywang@ncbi.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 80-CINF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851303606 ER PT J AU Weiss, DR Ahn, S Sassano, MF Kruse, AC Karpiak, J Roth, BL Kobilka, BK Shoichet, BK Lefkowitz, RJ AF Weiss, Dahlia R. Ahn, SeungKirl Sassano, Maria F. Kruse, Andrew C. Karpiak, Joel Roth, Bryan L. Kobilka, Brian K. Shoichet, Brian K. Lefkowitz, Robert J. TI G-protein coupled receptors in virtual screening: Functional fidelity and selectivity SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Weiss, Dahlia R.; Karpiak, Joel; Shoichet, Brian K.] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA. [Ahn, SeungKirl; Lefkowitz, Robert J.] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA. [Sassano, Maria F.; Roth, Bryan L.] Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC 27599 USA. [Sassano, Maria F.; Roth, Bryan L.] Univ N Carolina, Sch Med, NIMH, Psychoact Drug Screening Program, Chapel Hill, NC 27599 USA. [Kruse, Andrew C.; Kobilka, Brian K.] Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA. EM dahlia@blur.compbio.ucsf.edu RI Roth, Bryan/F-3928-2010 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 404-COMP PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851304408 ER PT J AU Zhao, J Wang, QM Zhao, C Hu, RD Nussinov, R Zheng, J AF Zhao, Jun Wang, Qiuming Zhao, Chao Hu, Rundong Nussinov, Ruth Zheng, Jie TI Investigating ion channel activity of human islet amyloid polypeptide (amylin) using molecular dynamics simulations SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 245th National Meeting of the American-Chemical-Society (ACS) CY APR 07-11, 2013 CL New Orleans, LA SP Amer Chem Soc C1 [Zhao, Jun; Wang, Qiuming; Zhao, Chao; Hu, Rundong; Zheng, Jie] Univ Akron, Dept Chem & Biomol Engn, Akron, OH 44325 USA. [Nussinov, Ruth] NCI, Basic Sci Program, SAIC Frederick Inc, Ctr Canc Res Nanobiol Program,Frederick Natl Lab, Frederick, MD 21702 USA. EM jz40@zips.uakron.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD APR 7 PY 2013 VL 245 MA 290-BIOT PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 210RD UT WOS:000323851301125 ER PT J AU Sitharaman, B Jacobson, BD Wadghiri, YZ Bryant, H Frank, J AF Sitharaman, Balaji Jacobson, Barry D. Wadghiri, Youssef Z. Bryant, Henry Frank, Joseph TI The magnetic, relaxometric, and optical properties of gadolinium-catalyzed single walled carbon nanotubes SO JOURNAL OF APPLIED PHYSICS LA English DT Article ID MRI CONTRAST AGENTS; HIGH-RELAXIVITY; GADOFULLERENES; GADONANOTUBES; NANOPARTICLES; COMPLEXES; NMR; FLUORESCENCE; RELAXATION; DYNAMICS AB We report the magnetic behavior, relaxometry, phantom magnetic resonance imaging (MRI), and near-infrared (NIR) photoluminescence spectroscopy of gadolinium (Gd) catalyzed single-walled carbon nanotubes (Gd-SWCNTs). Gd-SWCNTs are paramagnetic with an effective magnetic moment of 7.29 mu(B). Gd-SWCNT solutions show high r(1) and r(2) relaxivities at very low (0.01MHz) to clinically relevant (61MHz) magnetic fields (r(1) >= 130mM(-1) s(-1), r(2) >= 160 mM(-1) s(-1)). Analysis of nuclear magnetic resonance dispersion profiles using Solomon, Bloembergen, and Morgan equations suggests that multiple structural and dynamic parameters such as rotational correlation time tau(R), rate of water exchange tau(M), and the number of fast-exchanging water molecules within the inner sphere q may be responsible for the increase in r(1) and r(2) relaxivity. The T-1 weighted MRI signal intensity (gradient echo sequence; repetition time (TR) = 66ms, echo time (TE) = 3 ms, flop angle = 108 degrees) of Gd-SWCNT phantom solution is 14 times greater than the Gd-based clinical MRI contrast agent Magnevist. Additionally, these nanotubes exhibit near infrared fluorescence with distinct E-11 transitions of several semiconducting SWCNTs. Taken together, these results demonstrate that Gd-SWCNTs have potential as a novel, highly efficacious, multimodal MRI-NIR optical imaging contrast agent. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4796183] C1 [Sitharaman, Balaji; Jacobson, Barry D.] SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA. [Wadghiri, Youssef Z.] NYU, Bernard & Irene Schwartz Ctr Biomed Imaging, Dept Radiol, Sch Med, New York, NY 10016 USA. [Bryant, Henry; Frank, Joseph] NIH, Lab Diagnost Radiol Res, Bethesda, MD 20892 USA. [Frank, Joseph] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA. RP Sitharaman, B (reprint author), SUNY Stony Brook, Dept Biomed Engn, Bioengn Bldg,Room 115, Stony Brook, NY 11794 USA. EM balaji.sitharaman@stonybrook.edu RI Wadghiri, Youssef/E-6848-2016; OI Wadghiri, Youssef/0000-0001-7175-9397; Sitharaman, Balaji/0000-0001-8391-8076 FU Office of the Vice President of Research of Stony Brook University; Carol M. Baldwin Fund; National Institutes of Health [1DP2OD007394-01]; Department of Defense Breast Cancer Research Program [W81XWH-10-1-0521]; Clinical Center intramural research program at the National Institutes of Health FX This research was supported by the Office of the Vice President of Research of Stony Brook University, Carol M. Baldwin Fund, National Institutes of Health (Grant No. 1DP2OD007394-01), Department of Defense Breast Cancer Research Program (W81XWH-10-1-0521). This work was also supported in part by the Clinical Center intramural research program at the National Institutes of Health. NR 36 TC 13 Z9 13 U1 2 U2 39 PU AMER INST PHYSICS PI MELVILLE PA CIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA SN 0021-8979 J9 J APPL PHYS JI J. Appl. Phys. PD APR 7 PY 2013 VL 113 IS 13 AR 134308 DI 10.1063/1.4796183 PG 7 WC Physics, Applied SC Physics GA 121IZ UT WOS:000317238000049 PM 23653487 ER PT J AU Luis, AD Hayman, DTS O'Shea, TJ Cryan, PM Gilbert, AT Pulliam, JRC Mills, JN Timonin, ME Willis, CKR Cunningham, AA Fooks, AR Rupprecht, CE Wood, JLN Webb, CT AF Luis, Angela D. Hayman, David T. S. O'Shea, Thomas J. Cryan, Paul M. Gilbert, Amy T. Pulliam, Juliet R. C. Mills, James N. Timonin, Mary E. Willis, Craig K. R. Cunningham, Andrew A. Fooks, Anthony R. Rupprecht, Charles E. Wood, James L. N. Webb, Colleen T. TI A comparison of bats and rodents as reservoirs of zoonotic viruses: are bats special? SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE trait-based approaches; zoonoses; viral richness; reservoir host; spillover; Chiroptera ID EMERGING INFECTIOUS-DISEASES; PLACENTAL MAMMAL PHYLOGENY; WILD PRIMATES; LIFE-HISTORY; WEST-AFRICA; HOST-RANGE; EMERGENCE; ECOLOGY; EVOLUTION; PATTERNS AB Bats are the natural reservoirs of a number of high-impact viral zoonoses. We present a quantitative analysis to address the hypothesis that bats are unique in their propensity to host zoonotic viruses based on a comparison with rodents, another important host order. We found that bats indeed host more zoonotic viruses per species than rodents, and we identified life-history and ecological factors that promote zoonotic viral richness. More zoonotic viruses are hosted by species whose distributions overlap with a greater number of other species in the same taxonomic order (sympatry). Specifically in bats, there was evidence for increased zoonotic viral richness in species with smaller litters (one young), greater longevity and more litters per year. Furthermore, our results point to a new hypothesis to explain in part why bats host more zoonotic viruses per species: the stronger effect of sympatry in bats and more viruses shared between bat species suggests that interspecific transmission is more prevalent among bats than among rodents. Although bats host more zoonotic viruses per species, the total number of zoonotic viruses identified in bats (61) was lower than in rodents (68), a result of there being approximately twice the number of rodent species as bat species. Therefore, rodents should still be a serious concern as reservoirs of emerging viruses. These findings shed light on disease emergence and perpetuation mechanisms and may help lead to a predictive framework for identifying future emerging infectious virus reservoirs. C1 [Luis, Angela D.; Hayman, David T. S.; Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Luis, Angela D.; Pulliam, Juliet R. C.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Hayman, David T. S.; Wood, James L. N.] Univ Cambridge, Dept Vet Med, Dis Dynam Unit, Cambridge CB3 0ES, England. [Hayman, David T. S.; Fooks, Anthony R.] Anim Hlth & Vet Labs Agcy Weybridge, Wildlife Zoonoses & Vector Borne Dis Res Grp, Addlestone KT15 3NB, Surrey, England. [Hayman, David T. S.; Cunningham, Andrew A.] Zool Soc London, Inst Zool, London NW1 4RY, England. [O'Shea, Thomas J.] US Geol Survey, Ft Collins, CO 80526 USA. [Cryan, Paul M.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Gilbert, Amy T.] Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Pulliam, Juliet R. C.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Pulliam, Juliet R. C.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Mills, James N.] Emory Univ, Populat Biol Ecol & Evolut Program, Atlanta, GA 30322 USA. [Timonin, Mary E.; Willis, Craig K. R.] Univ Winnipeg, Dept Biol, Winnipeg, MB R3B 2E9, Canada. [Timonin, Mary E.; Willis, Craig K. R.] Univ Winnipeg, Ctr Forest Interdisciplinary Res, Winnipeg, MB R3B 2E9, Canada. [Fooks, Anthony R.] Natl Consortium Zoonosis Res, Neston CH64 7TE, South Wirral, England. [Rupprecht, Charles E.] Global Alliance Rabies Control, Manhattan, KS 66502 USA. RP Luis, AD (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM angela.d.luis@gmail.com RI Wood, James/A-1626-2008; Willis, Craig/F-5218-2013; APHA, Staff publications/E-6082-2010; Cunningham, Andrew/E-7536-2010; Fooks, Anthony/F-5418-2010; OI Wood, James/0000-0002-0258-3188; Pulliam, Juliet/0000-0003-3314-8223; Cryan, Paul/0000-0002-2915-8894 FU Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate (US Department of Homeland Security); Fogarty International Center (National Institutes of Health); Wellcome Trust; David H. Smith post-doctoral fellowship; Royal Society Wolfson Research Merit award; Alborada Trust; Natural Sciences and Engineering Research Council (NSERC Canada) FX This work was supported by the Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate (US Department of Homeland Security) and the Fogarty International Center (National Institutes of Health). D.T.S.H. acknowledges funding from the Wellcome Trust and a David H. Smith post-doctoral fellowship. A.A.C. is partially funded by a Royal Society Wolfson Research Merit award, and J.L.N.W. is supported by the Alborada Trust. C.K.R.W. is funded by a Discovery Grant from the Natural Sciences and Engineering Research Council (NSERC Canada). We thank Dan Horton for useful discussions. The findings and conclusions in this report are those of the authors and the US Geological Survey, but not of the other institutions. Any use of trade product or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 59 TC 119 Z9 126 U1 13 U2 206 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD APR 7 PY 2013 VL 280 IS 1756 AR 20122753 DI 10.1098/rspb.2012.2753 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA 097GH UT WOS:000315461500012 PM 23378666 ER PT J AU Bogich, TL Funk, S Malcolm, TR Chhun, N Epstein, JH Chmura, AA Kilpatrick, AM Brownstein, JS Hutchison, OC Doyle-Capitman, C Deaville, R Morse, SS Cunningham, AA Daszak, P AF Bogich, Tiffany L. Funk, Sebastian Malcolm, Trent R. Chhun, Nok Epstein, Jonathan H. Chmura, Aleksei A. Kilpatrick, A. Marm Brownstein, John S. Hutchison, O. Clyde Doyle-Capitman, Catherine Deaville, Robert Morse, Stephen S. Cunningham, Andrew A. Daszak, Peter TI Using network theory to identify the causes of disease outbreaks of unknown origin SO JOURNAL OF THE ROYAL SOCIETY INTERFACE LA English DT Article DE emerging infectious disease; encephalitis; complex networks; South Asia; cluster analysis; early warning systems ID INFECTIOUS-DISEASES; VIRUS-INFECTION; INFLUENZA; SURVEILLANCE; BANGLADESH; DIAGNOSIS; EMERGENCE; TRENDS; WORLD; WEB AB The identification of undiagnosed disease outbreaks is critical for mobilizing efforts to prevent widespread transmission of novel virulent pathogens. Recent developments in online surveillance systems allow for the rapid communication of the earliest reports of emerging infectious diseases and tracking of their spread. The efficacy of these programs, however, is inhibited by the anecdotal nature of informal reporting and uncertainty of pathogen identity in the early stages of emergence. We developed theory to connect disease outbreaks of known aetiology in a network using an array of properties including symptoms, seasonality and case-fatality ratio. We tested the method with 125 reports of outbreaks of 10 known infectious diseases causing encephalitis in South Asia, and showed that different diseases frequently form distinct clusters within the networks. The approach correctly identified unknown disease outbreaks with an average sensitivity of 76 per cent and specificity of 88 per cent. Outbreaks of some diseases, such as Nipah virus encephalitis, were well identified (sensitivity = 100%, positive predictive values = 80%), whereas others (e.g. Chandipura encephalitis) were more difficult to distinguish. These results suggest that unknown outbreaks in resource-poor settings could be evaluated in real time, potentially leading to more rapid responses and reducing the risk of an outbreak becoming a pandemic. C1 [Bogich, Tiffany L.; Malcolm, Trent R.; Chhun, Nok; Epstein, Jonathan H.; Chmura, Aleksei A.; Doyle-Capitman, Catherine; Daszak, Peter] EcoHlth Alliance, New York, NY 10001 USA. [Bogich, Tiffany L.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Bogich, Tiffany L.; Funk, Sebastian] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Funk, Sebastian; Hutchison, O. Clyde; Deaville, Robert; Cunningham, Andrew A.] Zool Soc London, Inst Zool, London NW1 4RY, England. [Funk, Sebastian] Univ London London Sch Hyg & Trop Med, London WC1E 7HT, England. [Kilpatrick, A. Marm] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Brownstein, John S.] Harvard Univ, Childrens Hosp Boston, Boston, MA 02115 USA. [Doyle-Capitman, Catherine] Amer Museum Nat Hist, Dept Mammal, New York, NY 10024 USA. [Morse, Stephen S.] Columbia Univ, Mailman Sch Publ Hlth, Dept Epidemiol, New York, NY 10032 USA. RP Bogich, TL (reprint author), EcoHlth Alliance, 460 W 34th St,17th Floor, New York, NY 10001 USA. EM tbogich@princeton.edu; sf7@princeton.edu; daszak@ecohealthalliance.org RI Cunningham, Andrew/E-7536-2010; OI Bogich, Tiffany/0000-0002-8143-5289; Funk, Sebastian/0000-0002-2842-3406 FU National Institutes of Health (NIH)/National Science Foundation (NSF); John E. Fogarty International Center [2R01-TW005869]; NSF [EF-0914866]; DTRA [HDTRA1-13-C-0029]; Rockefeller Foundation; New York Community Trust; Eppley Foundation for Research, Google.org; NIH [1R01AI090159-01]; NSF Human and Social Dynamics 'Agents of Change' award [BCS-0826779, BCS-0826840]; EU [231807] FX This study was made possible by the generous support of the American people through the United States Agency for International Development (USAID) Emerging Pandemic Threats PREDICT. The contents are the responsibility of the authors and do not necessarily reflect the views of USAID or the US Government. This work was also supported by National Institutes of Health (NIH)/National Science Foundation (NSF) 'Ecology of Infectious Diseases' awards from the John E. Fogarty International Center (2R01-TW005869), the NSF (EF-0914866), DTRA (HDTRA1-13-C-0029), the Rockefeller Foundation, the New York Community Trust, the Eppley Foundation for Research, Google.org, the NIH (1R01AI090159-01) and a NSF Human and Social Dynamics 'Agents of Change' award (BCS-0826779 & BCS-0826840). T.L.B. acknowledges the Research and Policy for Infectious Disease Dynamics program of the Science and Technology Directorate, U.S. Department of Homeland Security, and the Fogarty International Center, NIH for funding. S.F. acknowledges the EU FP7 funded integrated project EPIWORK (grant agreement no. 231807) for funding. The authors thank J. Bryden, L. Madoff, N. Wale, J. White and J. Zelner for assistance in preparing the manuscript. NR 35 TC 1 Z9 1 U1 0 U2 50 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1742-5689 J9 J R SOC INTERFACE JI J. R. Soc. Interface PD APR 6 PY 2013 VL 10 IS 81 AR 20120904 DI 10.1098/rsif.2012.0904 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 093NY UT WOS:000315199800002 PM 23389893 ER PT J AU Reiner, RC Perkins, TA Barker, CM Niu, TC Chaves, LF Ellis, AM George, DB Le Menach, A Pulliam, JRC Bisanzio, D Buckee, C Chiyaka, C Cummings, DAT Garcia, AJ Gatton, ML Gething, PW Hartley, DM Johnston, G Klein, EY Michael, E Lindsay, SW Lloyd, AL Pigott, DM Reisen, WK Ruktanonchai, N Singh, BK Tatem, AJ Kitron, U Hay, SI Scott, TW Smith, DL AF Reiner, Robert C., Jr. Perkins, T. Alex Barker, Christopher M. Niu, Tianchan Fernando Chaves, Luis Ellis, Alicia M. George, Dylan B. Le Menach, Arnaud Pulliam, Juliet R. C. Bisanzio, Donal Buckee, Caroline Chiyaka, Christinah Cummings, Derek A. T. Garcia, Andres J. Gatton, Michelle L. Gething, Peter W. Hartley, David M. Johnston, Geoffrey Klein, Eili Y. Michael, Edwin Lindsay, Steven W. Lloyd, Alun L. Pigott, David M. Reisen, William K. Ruktanonchai, Nick Singh, Brajendra K. Tatem, Andrew J. Kitron, Uriel Hay, Simon I. Scott, Thomas W. Smith, David L. TI A systematic review of mathematical models of mosquito-borne pathogen transmission: 1970-2010 SO JOURNAL OF THE ROYAL SOCIETY INTERFACE LA English DT Review DE infectious disease dynamics; vector-borne disease; epidemiology; dengue; West Nile; filariasis ID WEST-NILE-VIRUS; MALARIA TRANSMISSION; CLIMATE-CHANGE; DENGUE VIRUS; LYMPHATIC FILARIASIS; POPULATION-DYNAMICS; VECTORIAL CAPACITY; SIMULATION-MODEL; HUMAN MOVEMENT; AEDES-AEGYPTI AB Mathematical models of mosquito-borne pathogen transmission originated in the early twentieth century to provide insights into how to most effectively combat malaria. The foundations of the Ross-Macdonald theory were established by 1970. Since then, there has been a growing interest in reducing the public health burden of mosquito-borne pathogens and an expanding use of models to guide their control. To assess how theory has changed to confront evolving public health challenges, we compiled a bibliography of 325 publications from 1970 through 2010 that included at least one mathematical model of mosquito-borne pathogen transmission and then used a 79-part questionnaire to classify each of 388 associated models according to its biological assumptions. As a composite measure to interpret the multidimensional results of our survey, we assigned a numerical value to each model that measured its similarity to 15 core assumptions of the Ross-Macdonald model. Although the analysis illustrated a growing acknowledgement of geographical, ecological and epidemiological complexities in modelling transmission, most models during the past 40 years closely resemble the Ross-Macdonald model. Modern theory would benefit from an expansion around the concepts of heterogeneous mosquito biting, poorly mixed mosquito-host encounters, spatial heterogeneity and temporal variation in the transmission process. C1 [Reiner, Robert C., Jr.; Perkins, T. Alex; Barker, Christopher M.; Niu, Tianchan; Ellis, Alicia M.; George, Dylan B.; Pulliam, Juliet R. C.; Cummings, Derek A. T.; Gatton, Michelle L.; Hartley, David M.; Lindsay, Steven W.; Lloyd, Alun L.; Reisen, William K.; Tatem, Andrew J.; Kitron, Uriel; Hay, Simon I.; Scott, Thomas W.; Smith, David L.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Reiner, Robert C., Jr.; Perkins, T. Alex; Scott, Thomas W.] Univ Calif Davis, Sch Vet Med, Dept Entomol, Davis, CA 95616 USA. [Barker, Christopher M.; Reisen, William K.] Univ Calif Davis, Sch Vet Med, Ctr Vectorborne Dis, Davis, CA 95616 USA. [Barker, Christopher M.] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. [Niu, Tianchan; Hartley, David M.] Georgetown Univ, Med Ctr, Div Integrated Biodef, Washington, DC 20007 USA. [Hartley, David M.] Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20007 USA. [Fernando Chaves, Luis] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido, Japan. [Fernando Chaves, Luis] Hokkaido Univ, Global Ctr Excellence Program Integrated Field En, Sapporo, Hokkaido, Japan. [Fernando Chaves, Luis] Univ Nacl, Escuela Med Vet, Programa Invest Enfermedades Trop, Heredia, Costa Rica. [Fernando Chaves, Luis] Nagasaki Univ, Inst Trop Med NEKKEN, Nagasaki 852, Japan. [Fernando Chaves, Luis] Nagasaki Univ, Global Ctr Excellence Program Trop & Emergent Inf, Nagasaki 852, Japan. [Ellis, Alicia M.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT USA. [George, Dylan B.; Klein, Eili Y.] Dept Def, Ft Detrick, MD USA. [Le Menach, Arnaud; Smith, David L.] Ctr Dis Dynam Econ & Policy, Washington, DC USA. [Pulliam, Juliet R. C.; Chiyaka, Christinah; Garcia, Andres J.; Ruktanonchai, Nick] Univ Florida, Emerging Pathogens Inst, Gainesville, FL USA. [Pulliam, Juliet R. C.; Ruktanonchai, Nick] Univ Florida, Dept Biol, Gainesville, FL USA. [Garcia, Andres J.] Univ Florida, Dept Geog, Gainesville, FL 32611 USA. [Bisanzio, Donal; Kitron, Uriel] Emory Univ, Dept Environm Studies, Atlanta, GA 30322 USA. [Buckee, Caroline] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Ctr Communicable Dis Dynam, Boston, MA 02115 USA. [Cummings, Derek A. T.; Johnston, Geoffrey; Smith, David L.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Johnston, Geoffrey; Smith, David L.] Johns Hopkins Bloomberg Sch Publ Hlth, Malaria Res Inst, Baltimore, MD USA. [Gatton, Michelle L.] Queensland Inst Med Res, Malaria Drug Resistance & Chemotherapy Lab, Herston, Qld 4006, Australia. [Gething, Peter W.; Pigott, David M.; Hay, Simon I.] Univ Oxford, Dept Zool, Spatial Ecol & Epidemiol Grp, Oxford OX1 3PS, England. [Johnston, Geoffrey] Columbia Univ, Sch Int & Publ Affairs, New York, NY USA. [Johnston, Geoffrey] Columbia Univ, Coll Phys & Surg, Dept Microbiol & Immunol, New York, NY USA. [Klein, Eili Y.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Klein, Eili Y.] Johns Hopkins Univ, Dept Emergency Med, Ctr Adv Modeling, Baltimore, MD USA. [Michael, Edwin; Singh, Brajendra K.] Univ Notre Dame, Dept Biol Sci, Eck Inst Global Hlth, Notre Dame, IN 46556 USA. [Michael, Edwin] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London, England. [Lindsay, Steven W.] London Sch Hyg & Trop Med, Dept Dis Control, London WC1, England. [Lindsay, Steven W.] Univ Durham, Sch Biol & Biomed Sci, Durham, England. [Lloyd, Alun L.] N Carolina State Univ, Dept Math, Raleigh, NC 27695 USA. [Lloyd, Alun L.] N Carolina State Univ, Biomath Grad Program, Raleigh, NC 27695 USA. [Tatem, Andrew J.] Univ Southampton, Dept Geog & Environm, Southampton, Hants, England. RP Reiner, RC (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM rcreiner@ucdavis.edu; taperkins@ucdavis.edu RI sebastianovitsch, stepan/G-8507-2013; Smith, David/L-8850-2013; Lloyd, Alun/H-4944-2012; Michael, Edwin/D-3388-2014; Chaves, Luis Fernando/F-3448-2010; Singh, Brajendra/C-3787-2012; Hay, Simon/F-8967-2015 OI Pulliam, Juliet/0000-0003-3314-8223; , David/0000-0003-2589-2538; Gething, Peter/0000-0001-6759-5449; Pigott, David/0000-0002-6731-4034; Smith, David/0000-0003-4367-3849; Klein, Eili/0000-0002-1304-5289; Gatton, Michelle/0000-0003-1188-609X; Chaves, Luis Fernando/0000-0002-5301-2764; Singh, Brajendra/0000-0001-5847-8750; Hay, Simon/0000-0002-0611-7272 FU Research and Policy for Infectious Disease Dynamics programme of the Science and Technology Directory, Department of Homeland Security; Fogarty International Center, National Institutes of Health (NIH); US Center for Disease Control and Prevention [5 U01 EH000418]; Japan Society for the Promotion of Science; National Science Foundation [0801544]; Environment Program at the University of Florida; NIH [R01 AI069387-01A1, R01-AI091980, R01-AI069341, R01-GM08322]; Foundation for the NIH through the Grand Challenges in Global Health Initiative of the Bill and Melinda Gates Foundation; Bill and Melinda Gates Foundation [49446, 1032350, OPP52250]; Wellcome Trust [095066]; Wellcome Trust, UK; Innovative Vector Control Consortium; Bloomberg Family Foundation; NIH/NIAID [U19AI089674] FX This work was supported by the Research and Policy for Infectious Disease Dynamics programme of the Science and Technology Directory, Department of Homeland Security and Fogarty International Center, National Institutes of Health (NIH). C.M.B. acknowledges additional funding from the US Center for Disease Control and Prevention (5 U01 EH000418). L.F.C. is funded by a Fellowship from Japan Society for the Promotion of Science. A.J.G. was partially supported by the National Science Foundation under Grant No. 0801544 in the Quantitative Spatial Ecology, Evolution, and Environment Program at the University of Florida. E.M. and B.K.S. acknowledge funding from the NIH (R01 AI069387-01A1). A.L.L. acknowledges funding from the Foundation for the NIH through the Grand Challenges in Global Health Initiative of the Bill and Melinda Gates Foundation and from the NIH (R01-AI091980). A.J.T. and D.L.S. are also supported by grants from the Bill and Melinda Gates Foundation (49446 and 1032350). S.I.H. is also funded by a Senior Research Fellowship from the Wellcome Trust (095066), which also supports P.W.G. and D.M.P. This work forms part of the output of the Malaria Atlas Project, principally funded by the Wellcome Trust, UK. T.W.S. acknowledges funding from the Bill and Melinda Gates Foundation (OPP52250), the Innovative Vector Control Consortium and the NIH (R01-AI069341, R01-AI091980 and R01-GM08322). D.L.S. and G.J. received funding from the Bloomberg Family Foundation, and D.L.S., A.J.T. and G.J. acknowledge funding from NIH/NIAID (U19AI089674). We thank three anonymous reviewers for comments that improved the manuscript. NR 76 TC 85 Z9 86 U1 10 U2 193 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1742-5689 EI 1742-5662 J9 J R SOC INTERFACE JI J. R. Soc. Interface PD APR 6 PY 2013 VL 10 IS 81 AR 20120921 DI 10.1098/rsif.2012.0921 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 093NY UT WOS:000315199800005 PM 23407571 ER PT J AU Chong, WP Ling, MT Liu, YP Caspi, RR Wong, WM Wu, WT Tu, WW Lau, YL AF Chong, Wai Po Ling, Man To Liu, Yinping Caspi, Rachel R. Wong, Wai Man Wu, Wutian Tu, Wenwei Lau, Yu Lung TI Essential Role of NK Cells in IgG Therapy for Experimental Autoimmune Encephalomyelitis SO PLOS ONE LA English DT Article ID NATURAL-KILLER-CELLS; REGULATORY T-CELLS; FC-GAMMA-RECEPTORS; INTRAVENOUS IMMUNOGLOBULIN; DENDRITIC CELLS; IN-VIVO; DISEASE; RESPONSES; IL-2; ACTIVATION AB Intravenous immunoglobulin has long been used in treating autoimmune diseases, although mechanisms remain uncertain. Activating Fc gamma receptors are receptors of IgG and reported to be essential in intravenous immunoglobulin (IVIG) therapy. Therefore, we hypothesized natural killer (NK) cells, which express abundant activating Fc gamma receptors, are the potential cellular target. In experimental autoimmune encephalomyelitis (EAE), we demonstrated that IgG suppressed disease development in intact, but not in NK cell depleted mice. Adoptive transfer of IgG-treated NK cell could protect mice against EAE, and suppressed interferon c and interleukin 17 production. The percentage of CD4(+)Foxp3(+) regulatory T cells was significantly increased. The increase of regulatory T cells was also observed in IgG-treated EAE mice but not in NK cell depleted mice. In vitro experiments confirmed that IgG-treated NK cells enhanced regulatory T cell induction from naive CD4(+) T cells. Interestingly, cells from draining lymph nodes produced more interleukin 2 after the adoptive transfer of IgG-treated NK cells. We neutralized interleukin 2 and the induction of CD4(+)Foxp3(+) T cells by IgG-treated NK cells was significantly reduced. To our knowledge, we identified for the first time the critical role of NK cells in the mechanism of IgG-induced induction of Treg cells in treatment of autoimmunity. C1 [Chong, Wai Po; Ling, Man To; Liu, Yinping; Tu, Wenwei; Lau, Yu Lung] Univ Hong Kong, Dept Paediat & Adolescent Med, Li Ka Shing Fac Med, Pokfulam, Hong Kong, Peoples R China. [Chong, Wai Po; Caspi, Rachel R.] NEI, Immunol Lab, Bethesda, MD 20892 USA. [Wong, Wai Man; Wu, Wutian] Univ Hong Kong, Dept Anat, Li Ka Shing Fac Med, Pokfulam, Hong Kong, Peoples R China. RP Lau, YL (reprint author), Univ Hong Kong, Dept Paediat & Adolescent Med, Li Ka Shing Fac Med, Pokfulam, Hong Kong, Peoples R China. EM lauylung@hkucc.hku.hk OI Caspi, Rachel/0000-0002-7140-7671 FU Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR, China [A0E/M-12/06]; Edward Sai-Kim Hotung Paediatric Education and Research Fund; Chung Ko Lee and Cheung Yuen Kan Education and Research Fund in Paediatric Immunology; Shun Tak District Min Yuen Tong; Wong Ching Yee Memorial Postgraduate Scholarship; Mary Sun Medical Scholarship FX The study was partially supported by the Area of Excellence Scheme of the University Grants Committee, Hong Kong SAR, China (Grant A0E/M-12/06; YLL and WT), Edward Sai-Kim Hotung Paediatric Education and Research Fund (WPC and YLL), Chung Ko Lee and Cheung Yuen Kan Education and Research Fund in Paediatric Immunology (YLL), The Shun Tak District Min Yuen Tong (YLL), Wong Ching Yee Memorial Postgraduate Scholarship (WPC) and Mary Sun Medical Scholarship (WPC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 7 Z9 7 U1 1 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 5 PY 2013 VL 8 IS 4 AR e60862 DI 10.1371/journal.pone.0060862 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RZ UT WOS:000319109800108 PM 23577171 ER PT J AU Petrini, I Rajan, A Pham, T Voeller, D Davis, S Gao, J Wang, YS Giaccone, G AF Petrini, Iacopo Rajan, Arun Trung Pham Voeller, Donna Davis, Sean Gao, James Wang, Yisong Giaccone, Giuseppe TI Whole Genome and Transcriptome Sequencing of a B3 Thymoma SO PLOS ONE LA English DT Article ID ACUTE MYELOID-LEUKEMIA; SOMATIC MUTATIONS; TUMOR-SUPPRESSOR; BCOR; CANCER; CARCINOMA; PROTEINS; GENES AB Molecular pathology of thymomas is poorly understood. Genomic aberrations are frequently identified in tumors but no extensive sequencing has been reported in thymomas. Here we present the first comprehensive view of a B3 thymoma at whole genome and transcriptome levels. A 55-year-old Caucasian female underwent complete resection of a stage IVA B3 thymoma. RNA and DNA were extracted from a snap frozen tumor sample with a fraction of cancer cells over 80%. We performed array comparative genomic hybridization using Agilent platform, transcriptome sequencing using HiSeq 2000 (Illumina) and whole genome sequencing using Complete Genomics Inc platform. Whole genome sequencing determined, in tumor and normal, the sequence of both alleles in more than 95% of the reference genome (NCBI Build 37). Copy number (CN) aberrations were comparable with those previously described for B3 thymomas, with CN gain of chromosome 1q, 5, 7 and X and CN loss of 3p, 6, 11q42.2-qter and q13. One translocation t(11;X) was identified by whole genome sequencing and confirmed by PCR and Sanger sequencing. Ten single nucleotide variations (SNVs) and 2 insertion/deletions (INDELs) were identified; these mutations resulted in non-synonymous amino acid changes or affected splicing sites. The lack of common cancer-associated mutations in this patient suggests that thymomas may evolve through mechanisms distinctive from other tumor types, and supports the rationale for additional high-throughput sequencing screens to better understand the somatic genetic architecture of thymoma. C1 [Petrini, Iacopo; Rajan, Arun; Trung Pham; Voeller, Donna; Gao, James; Wang, Yisong; Giaccone, Giuseppe] NCI, Med Oncol Branch, Bethesda, MD 20892 USA. [Davis, Sean] NCI, Genet Branch, Bethesda, MD 20892 USA. RP Giaccone, G (reprint author), NCI, Med Oncol Branch, Bethesda, MD 20892 USA. EM giacconeg@mail.nih.gov RI Petrini, Iacopo/K-7316-2016; Giaccone, Giuseppe/E-8297-2017; OI Petrini, Iacopo/0000-0002-7752-6866; Giaccone, Giuseppe/0000-0002-5023-7562; Davis, Sean/0000-0002-8991-6458 FU National Cancer Institute intramural program FX National Cancer Institute intramural program funded this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 7 Z9 8 U1 1 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 5 PY 2013 VL 8 IS 4 AR e60572 DI 10.1371/journal.pone.0060572 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RZ UT WOS:000319109800064 PM 23577124 ER PT J AU Song, HF Janosko, K Johnson, RF Qin, J Josleyn, N Jett, C Byrum, R St Claire, M Dyall, J Blaney, JE Jennings, G Jahrling, PB AF Song, Haifeng Janosko, Krisztina Johnson, Reed F. Qin, Jing Josleyn, Nicole Jett, Catherine Byrum, Russell St Claire, Marisa Dyall, Julie Blaney, Joseph E. Jennings, Gerald Jahrling, Peter B. TI Poxvirus Antigen Staining of Immune Cells as a Biomarker to Predict Disease Outcome in Monkeypox and Cowpox Virus Infection in Non-Human Primates SO PLOS ONE LA English DT Article ID LETHAL MONKEYPOX; VACCINIA VIRUS; SMALLPOX VACCINATION; CYNOMOLGUS MONKEYS; PROTECTION; MODEL; PROGRESSION; CHALLENGE; MACAQUES AB Infection of non-human primates (NHPs) such as rhesus and cynomolgus macaques with monkeypox virus (MPXV) or cowpox virus (CPXV) serve as models to study poxvirus pathogenesis and to evaluate vaccines and anti-orthopox therapeutics. Intravenous inoculation of macaques with high dose of MPXV (>1-2 x 10(7) PFU) or CPXV (>10(2) PFU) results in 80% to 100% mortality and 66 to 100% mortality respectively. Here we report that NHPs with positive detection of poxvirus antigens in immune cells by flow cytometric staining, especially in monocytes and granulocytes succumbed to virus infection and that early positive pox staining is a strong predictor for lethality. Samples from four independent studies were analyzed. Eighteen NHPs from three different experiments were inoculated with two different MPXV strains at lethal doses. Ten NHPs displayed positive pox-staining and all 10 NHPs reached moribund endpoint. In contrast, none of the three NHPs that survived anticipated lethal virus dose showed apparent virus staining in the monocytes and granulocytes. In addition, three NHPs that were challenged with a lethal dose of MPXV and received cidofovir treatment were pox-antigen negative and all three NHPs survived. Furthermore, data from a CPXV study also demonstrated that 6/9 NHPs were pox-antigen staining positive and all 6 NHPs reached euthanasia endpoint, while the three survivors were pox-antigen staining negative. Thus, we conclude that monitoring pox-antigen staining in immune cells can be used as a biomarker to predict the prognosis of virus infection. Future studies should focus on the mechanisms and implications of the pox-infection of immune cells and the correlation between pox-antigen detection in immune cells and disease progression in human poxviral infection. C1 [Song, Haifeng; Janosko, Krisztina; Josleyn, Nicole; Jett, Catherine; Byrum, Russell; Dyall, Julie; Jennings, Gerald; Jahrling, Peter B.] NIAID, Integrated Res Facil, NIH, Frederick, MD USA. [Johnson, Reed F.; St Claire, Marisa; Blaney, Joseph E.; Jahrling, Peter B.] NIAID, Emerging Viral Pathogens Sect, NIH, Bethesda, MD 20892 USA. [Qin, Jing] NIAID, Biostat Res Branch, NIH, Bethesda, MD USA. RP Song, HF (reprint author), NIAID, Integrated Res Facil, NIH, Frederick, MD USA. EM songhai@mail.nih.gov FU NIAID (National Institute of Allergy and Infectious Diseases, National Institutes of Health) [HHSN272200200016I]; NIAID Division of Intramural Research FX The work was performed under Battelle Memorial contract with NIAID (National Institute of Allergy and Infectious Diseases, National Institutes of Health)(contract HHSN272200200016I). This study was funded, in part, by the NIAID Division of Intramural Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 22 TC 2 Z9 2 U1 0 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 5 PY 2013 VL 8 IS 4 AR e60533 DI 10.1371/journal.pone.0060533 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RZ UT WOS:000319109800060 PM 23577120 ER PT J AU Zanotti-Fregonara, P Hirvonen, J Lyoo, CH Zoghbi, SS Rallis-Frutos, D Huestis, MA Morse, C Pike, VW Innis, RB AF Zanotti-Fregonara, Paolo Hirvonen, Jussi Lyoo, Chul Hyoung Zoghbi, Sami S. Rallis-Frutos, Denise Huestis, Marilyn A. Morse, Cheryl Pike, Victor W. Innis, Robert B. TI Population-Based Input Function Modeling for [F-18]FMPEP-d(2), an Inverse Agonist Radioligand for Cannabinoid CB1 Receptors: Validation in Clinical Studies SO PLOS ONE LA English DT Article ID POSITRON-EMISSION-TOMOGRAPHY; CEREBRAL METABOLIC-RATE; BLOOD-SAMPLING METHOD; GLUCOSE-METABOLISM; BRAIN PET; FDG PET; IMAGE; QUANTIFICATION; BINDING AB Background: Population-based input function (PBIF) may be a valid alternative to full blood sampling for quantitative PET imaging. PBIF is typically validated by comparing its quantification results with those obtained via arterial sampling. However, for PBIF to be employed in actual clinical research studies, its ability to faithfully capture the whole spectrum of results must be assessed. The present study validated a PBIF for [F-18]FMPEP-d(2), a cannabinoid CB1 receptor radioligand, in healthy volunteers, and also attempted to utilize PBIF to replicate three previously published clinical studies in which the input function was acquired with arterial sampling. Methods: The PBIF was first created and validated with data from 42 healthy volunteers. This PBIF was used to assess the retest variability of [F-18]FMPEP-d(2), and then to quantify CB1 receptors in alcoholic patients (n = 18) and chronic daily cannabis smokers (n = 29). Both groups were scanned at baseline and after 2-4 weeks of monitored drug abstinence. Results: PBIF yielded accurate results in the 42 healthy subjects (average Logan-distribution volume (V-T) was 13.3 +/- 3.8 mL/cm(3) for full sampling and 13.2 +/- 3.8 mL/cm(3) for PBIF; R-2 = 0.8765, p < 0.0001) and test-retest results were comparable to those obtained with full sampling (variability: 16%; intraclass correlation coefficient: 0.89). PBIF accurately replicated the alcoholism study, showing a widespread similar to 20% reduction of CB1 receptors in alcoholic subjects, without significant change after abstinence. However, a small PBIF-V-T bias of -9% was unexpectedly observed in cannabis smokers. This bias led to substantial errors, including a V-T decrease in regions that had shown no downregulation in the full input function. Simulated data showed that the original findings could only have been replicated with a PBIF bias between -6% and +4%. Conclusions: Despite being initially well validated in healthy subjects, PBIF may misrepresent clinical protocol results and be a source of variability between different studies and institutions. C1 [Zanotti-Fregonara, Paolo; Hirvonen, Jussi; Lyoo, Chul Hyoung; Zoghbi, Sami S.; Rallis-Frutos, Denise; Morse, Cheryl; Pike, Victor W.; Innis, Robert B.] NIMH, Mol Imaging Branch, NIH, Bethesda, MD 20892 USA. [Huestis, Marilyn A.] NIDA, Chem & Drug Metab Sect, NIH, Baltimore, MD USA. RP Innis, RB (reprint author), NIMH, Mol Imaging Branch, NIH, Bethesda, MD 20892 USA. EM robert.innis@nih.gov FU National Institutes of Health FX This work was supported by the Intramural Research Program of the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 5 Z9 5 U1 0 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 5 PY 2013 VL 8 IS 4 AR e60231 DI 10.1371/journal.pone.0060231 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RZ UT WOS:000319109800036 PM 23577094 ER PT J AU Liu, Y Esser, L Interlandi, G Kisiela, DI Tchesnokova, V Thomas, WE Sokurenko, E Xia, D Savarino, SJ AF Liu, Yang Esser, Lothar Interlandi, Gianluca Kisiela, Dagmara I. Tchesnokova, Veronika Thomas, Wendy E. Sokurenko, Evgeni Xia, Di Savarino, Stephen J. TI Tight Conformational Coupling between the Domains of the Enterotoxigenic Escherichia coli Fimbrial Adhesin CfaE Regulates Binding State Transition SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID FACTOR ANTIGEN-I; STRUCTURAL BASIS; STRUCTURE VALIDATION; MOLECULAR-DYNAMICS; BACTERIAL ADHESION; CRYSTAL-STRUCTURE; CATCH BONDS; FIMH; MANNOSE; MOLPROBITY AB CfaE, the tip adhesin of enterotoxigenic Escherichia coli colonization factor antigen I fimbriae, initiates binding of this enteropathogen to the small intestine. It comprises stacked beta-sandwich adhesin (AD) and pilin (PD) domains, with the putative receptor-binding pocket at one pole and an equatorial interdomain interface. CfaE binding to erythrocytes is enhanced by application of moderate shear stress. A G168D replacement along the AD facing the CfaE interdomain region was previously shown to decrease the dependence on shear by increasing binding at lower shear forces. To elucidate the structural basis for this functional change, we studied the properties of CfaE G168D (with a self-complemented donor strand) and solved its crystal structure at 2.6 angstrom resolution. Compared with native CfaE, CfaE G168D showed a downward shift in peak erythrocyte binding under shear stress and greater binding under static conditions. The thermal melting transition of CfaE G168D occurred 10 C below that of CfaE. Compared with CfaE, the atomic structure of CfaE G168D revealed a 36% reduction in the buried surface area at the interdomain interface. Despite the location of this single modification in the AD, CfaE G168D exhibited structural derangements only in the adjoining PD compared with CfaE. In molecular dynamics simulations, the G168D mutation was associated with weakened interdomain interactions under tensile force. Taken together, these findings indicate that the AD and PD of CfaE are conformationally tightly coupled and support the hypothesis that opening of the interface plays a critical modulatory role in the allosteric activation of CfaE. C1 [Liu, Yang; Savarino, Stephen J.] USN, Enter Dis Dept, Infect Dis Directorate, Med Res Ctr, Silver Spring, MD 20910 USA. [Esser, Lothar; Xia, Di] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Esser, Lothar; Thomas, Wendy E.] Univ Washington, Dept Engn, Seattle, WA 98195 USA. [Kisiela, Dagmara I.; Tchesnokova, Veronika; Sokurenko, Evgeni] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA. [Savarino, Stephen J.] Uniformed Serv Univ Hlth Sci, Dept Pediat, Bethesda, MD 20814 USA. RP Sokurenko, E (reprint author), Univ Washington, Dept Microbiol, Hlth Sci Bldg,Rm E309, Seattle, WA 98195 USA. EM evs@u.washington.edu; dixia@helix.nih.gov; stephen.savarino@med.navy.mil FU National Institutes of Health [R01 AI050940]; National Cancer Institute Intramural Research Program; Trans-National Institutes of Health/Food and Drug Administration Intramural Biodefense Program; United States Army Military Infectious Diseases Research Program Work Unit [A1207]; Henry M. Jackson Foundation for the Advancement of Military Medicine; TeraGrid Grant [TG-MCB060069N]; National Science Foundation FX This work was supported, in whole or in part, by National Institutes of Health Grant R01 AI050940 (to E. S.) and by the National Cancer Institute Intramural Research Program and the Trans-National Institutes of Health/Food and Drug Administration Intramural Biodefense Program (to D. X.). This work was also supported by United States Army Military Infectious Diseases Research Program Work Unit A1207 and the Henry M. Jackson Foundation for the Advancement of Military Medicine (to S. J. S.). This work was prepared as part of the official duty of S. J. S.; We thank the staff of the Southeast Regional Collaborative Access Team for assistance for data collection. The molecular dynamics simulations were run on the Trestles supercomputer at the San Diego Supercomputer Center supported by TeraGrid Grant TG-MCB060069N with funding from the National Science Foundation. We are grateful to Daniel Isaac for critical reading of the manuscript. NR 31 TC 4 Z9 4 U1 1 U2 14 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 5 PY 2013 VL 288 IS 14 BP 9993 EP 10001 DI 10.1074/jbc.M112.413534 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 119RB UT WOS:000317114000038 PM 23393133 ER PT J AU Miles, EW AF Miles, Edith Wilson TI The Tryptophan Synthase alpha(2)beta(2) Complex: A Model for Substrate Channeling, Allosteric Communication, and Pyridoxal Phosphate Catalysis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ALPHA-METHYLSERINE HYDROXYMETHYLTRANSFERASE; ASPARTATE BETA-DECARBOXYLASE; ESCHERICHIA-COLI; SALMONELLA-TYPHIMURIUM; BIENZYME COMPLEX; B-PROTEIN; ALPHA-2-BETA-2 COMPLEX; ENZYMATIC-ACTIVITIES; MONOVALENT CATIONS; SUBUNIT AB I reflect on my research on pyridoxal phosphate (PLP) enzymes over fifty-five years and on how I combined research with marriage and family. My Ph.D. research with Esmond E. Snell established one aspect of PLP enzyme mechanism. My postdoctoral work first with Hans L. Kornberg and then with Alton Meister characterized the structure and function of another PLP enzyme, L-aspartate beta-decarboxylase. My independent research at the National Institutes of Health (NIH) since 1966 has focused on the bacterial tryptophan synthase alpha(2)beta(2) complex. The beta subunit catalyzes a number of PLP-dependent reactions. We have characterized these reactions and the allosteric effects of the alpha subunit. We also used chemical modification to probe enzyme structure and function. Our crystallization of the tryptophan synthase alpha(2)beta(2) complex from Salmonella typhimurium led to the determination of the three-dimensional structure with Craig Hyde and David Davies at NIH in 1988. This landmark structure was the first structure of a multienzyme complex and the first structure revealing an intramolecular tunnel. The structure has provided a basis for exploring mechanisms of catalysis, channeling, and allosteric communication in the tryptophan synthase alpha(2)beta(2) complex. The structure serves as a model for many other multiprotein complexes that are important for biological processes in prokaryotes and eukaryotes. C1 NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. RP Miles, EW (reprint author), NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. EM edithm@intra.niddk.nih.gov NR 36 TC 5 Z9 5 U1 4 U2 19 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 5 PY 2013 VL 288 IS 14 BP 10084 EP 10091 DI 10.1074/jbc.X113.463331 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 119RB UT WOS:000317114000048 PM 23426371 ER PT J AU Temporini, C Pochetti, G Fracchiolla, G Piemontese, L Montanari, R Moaddel, R Laghezza, A Altieri, F Cervoni, L Ubiali, D Prada, E Loiodice, F Massolini, G Calleri, E AF Temporini, C. Pochetti, G. Fracchiolla, G. Piemontese, L. Montanari, R. Moaddel, R. Laghezza, A. Altieri, F. Cervoni, L. Ubiali, D. Prada, E. Loiodice, F. Massolini, G. Calleri, E. TI Open tubular columns containing the immobilized ligand binding domain of peroxisome proliferator-activated receptors alpha and gamma for dual agonists characterization by frontal affinity chromatography with mass spectrometry detection SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article DE High-performance affinity chromatography; Frontal affinity chromatography coupled to mass spectrometry; Isothermal titration calorimetry; Immobilized PPAR gamma and PPAR alpha receptors; Ligand affinity determination ID PROTEIN-PROTEIN INTERACTIONS; DRUG DISCOVERY; STATIONARY-PHASE; TARGET; ASSAY; RISK AB The peroxisome proliferator-activated receptors (PPARs) belong to the nuclear receptor superfamily. In the last years novel PPARs ligands have been identified and these include PPAR alpha/gamma dual agonists. To rapidly identify novel PPARs dual ligands, a robust binding assay amenable to high-throughput screening toward PPAR isoforms would be desirable. In this work we describe a parallel assay based on the principles of frontal affinity chromatography coupled to mass spectrometry (FAC-MS) that can be used to characterize dual agonists. For this purpose the ligand binding domain of PPAR alpha receptor was immobilized onto the surface of open tubular capillaries to create new PPAR-alpha-OT columns to be used in parallel with PPAR-gamma-OT columns. The two biochromatographic systems were used in both ranking and K-d experiments toward new ureidofibrate-like dual agonists for subtype selectivity ratio determination. In order to validate the system, the K-d values determined by frontal analysis chromatography were compared to the affinity constants obtained by ITC experiments. The results of this study strongly demonstrate the specific nature of the interaction of the ligands with the two immobilized receptor subtypes. (C) 2013 Elsevier B.V. All rights reserved. C1 [Temporini, C.; Ubiali, D.; Prada, E.; Massolini, G.; Calleri, E.] Univ Pavia, Dipartimento Sci Farmaco, I-27100 Pavia, Italy. [Pochetti, G.; Montanari, R.] CNR, Ist Cristallog, Monterotondo Stn, I-00015 Rome, Italy. [Fracchiolla, G.; Piemontese, L.; Laghezza, A.; Loiodice, F.] Univ Bari Aldo Moro, Dipartimento Farm, I-70126 Bari, Italy. [Moaddel, R.] NIA, Biomed Res Ctr, NIH, Baltimore, MD 21224 USA. [Altieri, F.; Cervoni, L.] Univ Roma La Sapienza, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy. RP Calleri, E (reprint author), Univ Pavia, Dipartimento Sci Farmaco, Via Palestro 3, I-27100 Pavia, Italy. EM enrica.calleri@unipv.it RI FRACCHIOLLA, Giuseppe/P-3899-2015; Altieri, Fabio/D-2790-2009; OI FRACCHIOLLA, Giuseppe/0000-0003-4991-3962; Altieri, Fabio/0000-0002-6546-2738; CALLERI, ENRICA/0000-0002-4246-460X; Temporini, Caterina/0000-0003-1925-7845 FU Ministero dell'Universita e della Ricerca Scientifica [2009Z8YTYC]; Intramural Research Program at the National Institute on Aging, NIH; Universita degli Studi di Bari 'Aldo Moro' [GRBA1107J2] FX This work was supported by grant from Ministero dell'Universita e della Ricerca Scientifica (grant no. 2009Z8YTYC) and in part by the Intramural Research Program at the National Institute on Aging, NIH (RM). This work was also accomplished thanks to the financial support of the Universita degli Studi di Bari 'Aldo Moro' (Research Fund: "IDEA Giovani Ricercatori" GRBA1107J2). NR 23 TC 9 Z9 9 U1 0 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR A JI J. Chromatogr. A PD APR 5 PY 2013 VL 1284 BP 36 EP 43 DI 10.1016/j.chroma.2013.01.077 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 120EO UT WOS:000317153800005 PM 23466198 ER PT J AU Apps, R Qi, Y Carlson, JM Chen, HY Gao, XJ Thomas, R Yuki, Y Del Prete, GQ Goulder, P Brumme, ZL Brumme, CJ John, M Mallal, S Nelson, G Bosch, R Heckerman, D Stein, JL Soderberg, KA Moody, MA Denny, TN Zeng, X Fang, JY Moffett, A Lifson, JD Goedert, JJ Buchbinder, S Kirk, GD Fellay, J McLaren, P Deeks, SG Pereyra, F Walker, B Michael, NL Weintrob, A Wolinsky, S Liao, W Carrington, M AF Apps, Richard Qi, Ying Carlson, Jonathan M. Chen, Haoyan Gao, Xiaojiang Thomas, Rasmi Yuki, Yuko Del Prete, Greg Q. Goulder, Philip Brumme, Zabrina L. Brumme, Chanson J. John, Mina Mallal, Simon Nelson, George Bosch, Ronald Heckerman, David Stein, Judy L. Soderberg, Kelly A. Moody, M. Anthony Denny, Thomas N. Zeng, Xue Fang, Jingyuan Moffett, Ashley Lifson, Jeffrey D. Goedert, James J. Buchbinder, Susan Kirk, Gregory D. Fellay, Jacques McLaren, Paul Deeks, Steven G. Pereyra, Florencia Walker, Bruce Michael, Nelson L. Weintrob, Amy Wolinsky, Steven Liao, Wilson Carrington, Mary TI Influence of HLA-C Expression Level on HIV Control SO SCIENCE LA English DT Article ID INFLAMMATORY-BOWEL-DISEASE; T-CELL RESPONSES; DETERMINANTS; ASSOCIATIONS; METAANALYSIS; ALLELES; SYNAPSE; ESCAPE; NUMBER AB A variant upstream of human leukocyte antigen C (HLA-C) shows the most significant genome-wide effect on HIV control in European Americans and is also associated with the level of HLA-C expression. We characterized the differential cell surface expression levels of all common HLA-C allotypes and tested directly for effects of HLA-C expression on outcomes of HIV infection in 5243 individuals. Increasing HLA-C expression was associated with protection against multiple outcomes independently of individual HLA allelic effects in both African and European Americans, regardless of their distinct HLA-C frequencies and linkage relationships with HLA-B and HLA-A. Higher HLA-C expression was correlated with increased likelihood of cytotoxic T lymphocyte responses and frequency of viral escape mutation. In contrast, high HLA-C expression had a deleterious effect in Crohn's disease, suggesting a broader influence of HLA expression levels in human disease. C1 [Apps, Richard; Qi, Ying; Gao, Xiaojiang; Thomas, Rasmi; Yuki, Yuko; Carrington, Mary] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Expt Immunol Lab, Canc & Inflammat Program, Frederick, MD 21702 USA. [Apps, Richard; Qi, Ying; Gao, Xiaojiang; Yuki, Yuko; Goulder, Philip; Pereyra, Florencia; Walker, Bruce; Carrington, Mary] Massachusetts Gen Hosp, MIT, Ragon Inst, Boston, MA 02114 USA. [Apps, Richard; Qi, Ying; Gao, Xiaojiang; Yuki, Yuko; Goulder, Philip; Pereyra, Florencia; Walker, Bruce; Carrington, Mary] Harvard Univ, Boston, MA 02114 USA. [Carlson, Jonathan M.; Heckerman, David; Zeng, Xue] Microsoft Res, ESci Grp, Los Angeles, CA 90024 USA. [Chen, Haoyan; Fang, Jingyuan; Liao, Wilson] Univ Calif San Francisco, Dept Dermatol, San Francisco, CA 94115 USA. [Chen, Haoyan; Lifson, Jeffrey D.] Shanghai Jiao Tong Univ, Sch Med, Shanghai Inst Digest Dis, Dept Gastroenterol,Ren Ji Hosp, Shanghai 200001, Peoples R China. [Del Prete, Greg Q.] SAIC Frederick Inc, Frederick Natl Lab Canc Res, AIDS & Canc Virus Program, Frederick, MD 21702 USA. [Goulder, Philip] Univ KwaZulu Natal, Nelson R Mandela Sch Med, Doris Duke Med Res Inst, HIV Pathogenesis Programme, Durban, South Africa. [Goulder, Philip] Univ Oxford, Dept Paediat, Oxford OX1 3SY, England. [Brumme, Zabrina L.; Brumme, Chanson J.] British Columbia Ctr Excellence HIV AIDS, Vancouver, BC V6Z 1Y6, Canada. [Brumme, Zabrina L.] Simon Fraser Univ, Fac Hlth Sci, Burnaby, BC V5A 1S6, Canada. [John, Mina; Mallal, Simon] Murdoch Univ, Inst Immunol & Infect Dis, Murdoch, WA 6150, Australia. [Nelson, George] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Ctr Canc Res Genet Core, Basic Res Program, Frederick, MD 21702 USA. [Bosch, Ronald] Harvard Univ, Sch Publ Hlth, Ctr Biostat AIDS Res, Boston, MA 02115 USA. [Stein, Judy L.; Soderberg, Kelly A.; Moody, M. Anthony; Denny, Thomas N.] Duke Univ, Duke Human Vaccine Inst, Durham, NC 27710 USA. [Zeng, Xue] China Acad Chinese Med Sci, Guanganmen Hosp, Dept Dermatol, Beijing 100053, Peoples R China. [Moffett, Ashley] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England. [Goedert, James J.] NCI, Div Canc Epidemiol & Genet, Infect & Immunoepidemiol Branch, Bethesda, MD 20892 USA. [Buchbinder, Susan] San Francisco Dept Publ Hlth, San Francisco, CA 94102 USA. [Kirk, Gregory D.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21205 USA. [Fellay, Jacques; McLaren, Paul] Univ Lausanne Hosp, Inst Microbiol, CH-1015 Lausanne, Switzerland. [Fellay, Jacques; McLaren, Paul] Ecole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland. [Deeks, Steven G.] Univ Calif San Francisco, Dept Med, San Francisco, CA 94105 USA. [Michael, Nelson L.] Walter Reed Army Inst Res, US Mil HIV Res Program, Silver Spring, MD 20910 USA. [Weintrob, Amy] Uniformed Serv Univ Hlth Sci, Infect Dis Clin Res Program, Bethesda, MD 20817 USA. [Wolinsky, Steven] Northwestern Univ, Feinberg Sch Med, Div Infect Dis, Chicago, IL 60611 USA. RP Carrington, M (reprint author), SAIC Frederick Inc, Frederick Natl Lab Canc Res, Expt Immunol Lab, Canc & Inflammat Program, Frederick, MD 21702 USA. EM carringm@mail.nih.gov RI Del Prete, Gregory/C-2030-2012; Moffett, Ashley/J-9754-2013; SHCS, int. coll. A/G-4083-2011; Fellay, Jacques/A-6681-2009; SHCS, all/G-4072-2011; OI Fellay, Jacques/0000-0002-8240-939X; Denny, Thomas/0000-0002-7364-8276; Wolinsky, Steven/0000-0002-9625-6697; Moody, Tony/0000-0002-3890-5855; Brumme, Chanson/0000-0003-2722-5288 FU National Institutes of Health (NIH) [HHSN261200800001E, N02-CP-55504, RO1-AI046995, R01-DA04334, R01-DA12568, R01-AI060460]; Bill & Melinda Gates Foundation [38599]; Mark and Lisa Schwartz Foundation; Wellcome Trust; NIH National Institute of Allergy and Infectious Diseases (NIAID) Center for HIV/AIDS Vaccine Immunology [U01-AI-067854]; Australian National Health and Medical Research Council [384702]; National Institutes of Health [K08AR057763, R01-DA-04334, R01-DA-12568]; Creative and Novel Ideas in HIV Research Program [P30 AI027767-24]; Swiss National Science Foundation [33CSC0-108787]; NIAID; National Cancer Institute; National Heart, Lung, and Blood Institute [UO1-AI-35042, 5-MO1-RR-00722 (GCRC), UO1-AI-35043, UO1-AI-37984, UO1-AI-35039, UO1-AI-35040, UO1-AI-37613, UO1-AI-35041]; [RO1 AI087145]; [K24AI069994]; [P30 AI027763]; [UL1 RR024131]; [P30 MH62246]; [R24 AI067039] FX This study makes use of data generated by the Wellcome Trust Case Control Consortium (WTCCC); a full list of investigators who contributed to the generation of this data is available at www.wtccc.org.uk. The data reported in this paper are tabulated in the main paper and supplementary materials. Data and materials will be shared subject to institutional review board (IRB) provisions regarding patient privacy. Please contact the corresponding author. Those interested in obtaining Center for HIV/AIDS Vaccine Immunology (CHAVI) samples used in this study should contact M. C. and M. A. M. A Simple Letter Agreement for the Transfer of Materials will be executed between the institutions involved, and an IRB approval/exemption should be obtained by the recipient institution when appropriate. Clinical specimens from the Multicenter AIDS Cohort Study (MACS) are subject to the terms and conditions of the MACS Master Material Transfer Agreement for Non-Profit Institutions. The project has been funded in part with federal funds from the National Institutes of Health (NIH) under contracts HHSN261200800001E, N02-CP-55504, RO1-AI046995, R01-DA04334, R01-DA12568, and R01-AI060460. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services or the Department of Defense, nor does mention of trade names, commercial products, or organizations imply endorsement by the U. S. Government. This research was supported in part by grants from the Bill & Melinda Gates Foundation as part of the Collaboration for AIDS Vaccine Discovery and grant ID38599, the Mark and Lisa Schwartz Foundation, the Wellcome Trust, the NIH National Institute of Allergy and Infectious Diseases (NIAID) Center for HIV/AIDS Vaccine Immunology (U01-AI-067854), and the Australian National Health and Medical Research Council (Program Grant 384702). Further funding was from the National Institutes of Health grant K08AR057763 and the Creative and Novel Ideas in HIV Research Program through a supplement to the University of Alabama at the Birmingham Center For AIDS Research funding (P30 AI027767-24), made possible by collaborative efforts of the Office of AIDS Research, NIAID, and the International AIDS Society. We thank M. Trubey and A. Lara for cytometry assistance and all the patients and investigators contributing to samples studied in the WTCCC, CHAVI, Multicenter Hemophilia Cohort Study, San Francisco City Clinic Cohort, Massachusetts General Hospital Controller Cohort, United States Military HIV Natural History Study, DC Gay Cohort Study, British Columbia HOMER cohort, Western Australian HIV Cohort Study, US AIDS Clinical Trials Group, Durban, AIDS Linked to Intravenous Experience (supported by National Institutes of Health grants R01-DA-04334 and R01-DA-12568), Swiss HIV Cohort Study (supported by the Swiss National Science Foundation grant number 33CSC0-108787), Study on the Consequences of Protease Inhibitor Era (supported by grants RO1 AI087145, K24AI069994, P30 AI027763, UL1 RR024131, P30 MH62246, and R24 AI067039), and MACS cohorts (funded by NIAID, with supplemental funding from the National Cancer Institute and the National Heart, Lung, and Blood Institute [grants UO1-AI-35042, 5-MO1-RR-00722 (GCRC), UO1-AI-35043, UO1-AI-37984, UO1-AI-35039, UO1-AI-35040, UO1-AI-37613, and UO1-AI-35041]. NR 25 TC 117 Z9 118 U1 2 U2 51 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD APR 5 PY 2013 VL 340 IS 6128 BP 87 EP 91 DI 10.1126/science.1232685 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 118XS UT WOS:000317061100052 PM 23559252 ER PT J AU Bevilacqua, L Goldman, D AF Bevilacqua, Laura Goldman, David TI Genetics of impulsive behaviour SO PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Review DE impulsivity; heritability; genes; sequencing; HTR2B ID ATTENTION-DEFICIT/HYPERACTIVITY DISORDER; SEROTONIN TRANSPORTER GENE; GENOME-WIDE ASSOCIATION; RISK-TASK BART; FLUID 5-HYDROXYINDOLEACETIC ACID; NUCLEUS-ACCUMBENS CORE; 5-HT1B RECEPTOR GENE; RESPONSE-INHIBITION; ANTISOCIAL-BEHAVIOR; DECISION-MAKING AB Impulsivity, defined as the tendency to act without foresight, comprises a multitude of constructs and is associated with a variety of psychiatric disorders. Dissecting different aspects of impulsive behaviour and relating these to specific neurobiological circuits would improve our understanding of the etiology of complex behaviours for which impulsivity is key, and advance genetic studies in this behavioural domain. In this review, we will discuss the heritability of some impulsivity constructs and their possible use as endophenotypes (heritable, disease-associated intermediate phenotypes). Several functional genetic variants associated with impulsive behaviour have been identified by the candidate gene approach and re-sequencing, and whole genome strategies can be implemented for discovery of novel rare and common alleles influencing impulsivity. Via deep sequencing an uncommon HTR2B stop codon, common in one population, was discovered, with implications for understanding impulsive behaviour in both humans and rodents and for future gene discovery. C1 [Bevilacqua, Laura] NYU, Sch Med, Dept Psychiat, New York, NY 10016 USA. [Goldman, David] NIAAA, Neurogenet Lab, NIH, Rockville, MD 20852 USA. RP Bevilacqua, L (reprint author), NYU, Sch Med, Dept Psychiat, New York, NY 10016 USA. EM laura.bevilacqua@nyumc.org RI Goldman, David/F-9772-2010 OI Goldman, David/0000-0002-1724-5405 NR 146 TC 13 Z9 14 U1 5 U2 58 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8436 J9 PHILOS T R SOC B JI Philos. Trans. R. Soc. B-Biol. Sci. PD APR 5 PY 2013 VL 368 IS 1615 SI SI AR 20120380 DI 10.1098/rstb.2012.0380 PG 12 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 096BA UT WOS:000315377500006 PM 23440466 ER PT J AU Murphy, DL Moya, PR Fox, MA Rubenstein, LM Wendland, JR Timpano, KR AF Murphy, Dennis L. Moya, Pablo R. Fox, Meredith A. Rubenstein, Liza M. Wendland, Jens R. Timpano, Kiara R. TI Anxiety and affective disorder comorbidity related to serotonin and other neurotransmitter systems: obsessive - compulsive disorder as an example of overlapping clinical and genetic heterogeneity SO PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Review DE clinical heterogeneity; genetic heterogeneity; comorbidity; obsessive-compulsive disorder; serotonin ID POSTTRAUMATIC-STRESS-DISORDER; OCD COLLABORATIVE GENETICS; OPEN-LABEL TRIAL; COMPLEX SEGREGATION ANALYSIS; FAMILY-BASED ASSOCIATION; MYOCLONUS-DYSTONIA SYNDROME; TRAUMATIC BRAIN-INJURY; CARDIO-FACIAL SYNDROME; LA-TOURETTE-SYNDROME; TRANSPORTER GENE AB Individuals with obsessive-compulsive disorder (OCD) have also been shown to have comorbid lifetime diagnoses of major depressive disorder (MDD; rates greater than 70%), bipolar disorder (rates greater than 10%) and other anxiety disorders (e.g. panic disorder, post-traumatic stress disorder (PTSD)). In addition, overlap exists in some common genetic variants (e.g. the serotonin transporter gene (SLC6A4), the brain-derived neurotrophic factor (BDNF) gene), and rare variants in genes/chromosomal abnormalities (e.g. the 22q11 microdeletion syndrome) found across the affective/anxiety disorder spectrums. OCD has been proposed as a possible independent entity for DSM-5, but by others thought best retained as an anxiety disorder subtype (its current designation in DSM-IV), and yet by others considered best in the affective disorder spectrum. This review focuses on OCD, a well-studied but still puzzling heterogeneous disorder, regarding alterations in serotonergic, dopaminergic and glutamatergic neurotransmission in addition to other systems involved, and how related genes may be involved in the comorbidity of anxiety and affective disorders. OCD resembles disorders such as depression, in which gene x gene interactions, gene x environment interactions and stress elements coalesce to yield OC symptoms and, in some individuals, full-blown OCD with multiple comorbid disorders. C1 [Murphy, Dennis L.; Moya, Pablo R.; Fox, Meredith A.; Rubenstein, Liza M.; Wendland, Jens R.] NIMH, Clin Sci Lab, NIH, Bethesda, MD 20892 USA. [Timpano, Kiara R.] Univ Miami, Dept Psychol, Coral Gables, FL 33146 USA. RP Fox, MA (reprint author), NIMH, Clin Sci Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. EM mfox@mail.nih.gov OI Timpano, Kiara/0000-0002-0665-8722 FU NIMH Intramural Research Program FX The authors thank Diane Kazuba and Lucy Justement for conducting patient interviews, and Theresa Deguzman for her assistance with this manuscript. This research was supported by the NIMH Intramural Research Program. NR 252 TC 26 Z9 27 U1 10 U2 74 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8436 EI 1471-2970 J9 PHILOS T R SOC B JI Philos. Trans. R. Soc. B-Biol. Sci. PD APR 5 PY 2013 VL 368 IS 1615 SI SI AR 20120435 DI 10.1098/rstb.2012.0435 PG 18 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 096BA UT WOS:000315377500008 PM 23440468 ER PT J AU Guha, P Morgan, JW Mostoslavsky, G Rodrigues, NP Boyd, AS AF Guha, Prajna Morgan, John W. Mostoslavsky, Gustavo Rodrigues, Neil P. Boyd, Ashleigh S. TI Lack of Immune Response to Differentiated Cells Derived from Syngeneic Induced Pluripotent Stem Cells SO CELL STEM CELL LA English DT Article ID MOUSE AB The prospects for using autologous induced pluripotent stem cells (iPSCs) in cell replacement therapy have been tempered by evidence that undifferentiated, syngeneic mouse iPSCs are immunogenic upon transplantation. However, the immunogenicity of more therapeutically relevant differentiated cells remains unexplored. Here, we differentiated mouse iPSCs into embryoid bodies (EBs) or representative cell types spanning the three embryonic germ layers and assessed their immunogenicity in vitro and after their transplantation into syngeneic recipients. We found no evidence of increased T cell proliferation in vitro, rejection of syngeneic iPSC-derived EBs/tissue-specific cells (TSCs) after transplantation, or an antigen-specific secondary immune response. Thus, differentiated cells derived from syngeneic iPSCs do not appear to be rejected after transplantation. We also found little evidence of an immune response to undifferentiated, syngeneic iPSCs. Our data support the idea that differentiated cells generated from autologous iPSCs could be applied for cell replacement therapy without eliciting immune rejection. C1 [Guha, Prajna; Morgan, John W.; Rodrigues, Neil P.; Boyd, Ashleigh S.] Boston Univ, Sch Med, Roger Williams Med Ctr, NIH Ctr Biomed Res Excellence COBRE Stem Cell Bio, Providence, RI 02908 USA. [Mostoslavsky, Gustavo; Rodrigues, Neil P.; Boyd, Ashleigh S.] Boston Univ, Sch Med, Ctr Regenerat Med CReM, Boston, MA 02118 USA. [Rodrigues, Neil P.; Boyd, Ashleigh S.] Boston Univ, Sch Med, Dept Dermatol, Boston, MA 02118 USA. RP Boyd, AS (reprint author), Boston Univ, Sch Med, Roger Williams Med Ctr, NIH Ctr Biomed Res Excellence COBRE Stem Cell Bio, Providence, RI 02908 USA. EM asboyd@bu.edu FU NIH [P20RR018757]; Rhode Island Foundation; BD Biosciences FX This work was supported by NIH Grant P20RR018757 (A. S. B. and N.P.R.), the Rhode Island Foundation (A. S. B.), and BD Biosciences (N.P.R.). Additional support to A. S. B. and N.P.R. laboratories was provided by the administrative, imaging, and flow cytometry cores within the NIH COBRE at Roger Williams Medical Center. The authors thank Dr. Yang Xu for generously sharing the episomal iPSC line used in this study, Dr. Darrell Kotton for his advice on derivation of mouse iPSCs, Anthony Berghelli for editorial and technical assistance, and Jasmine Sayegh and Dr. Murugabaskar Balan for technical assistance. The authors declare no conflict of interest. P. G. performed experiments, acquired and analyzed data, and prepared figures; J.W.M. performed cell sorting and assisted with flow cytometry analysis; G. M. contributed vital reagents and advice on derivation of iPSC lines and reviewed the manuscript; N.P.R. conceived the project, designed experiments, analyzed and interpreted data, and wrote the manuscript; and A. S. B. conceived the project, designed experiments, contributed to derivation of iPSC lines, analyzed and interpreted data, and wrote the manuscript. NR 17 TC 145 Z9 152 U1 5 U2 52 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1934-5909 EI 1875-9777 J9 CELL STEM CELL JI Cell Stem Cell PD APR 4 PY 2013 VL 12 IS 4 BP 407 EP 412 DI 10.1016/j.stem.2013.01.006 PG 6 WC Cell & Tissue Engineering; Cell Biology SC Cell Biology GA 287VD UT WOS:000329569500008 PM 23352605 ER PT J AU James, AM Hsu, HT Dongre, P Uzel, G Mace, EM Banerjee, PP Orange, JS AF James, Ashley Mentlik Hsu, Hsiang-Ting Dongre, Prachi Uzel, Gulbu Mace, Emily M. Banerjee, Pinaki P. Orange, Jordan S. TI Rapid activation receptor- or IL-2-induced lytic granule convergence in human natural killer cells requires Src, but not downstream signaling SO BLOOD LA English DT Article ID MICROTUBULE-ORGANIZING CENTER; ALDRICH-SYNDROME PROTEIN; NK CELLS; IMMUNOLOGICAL SYNAPSE; CELLULAR CYTOTOXICITY; T-CELLS; GRB2; IL-2; PHOSPHORYLATION; ACTIN AB Natural killer (NK) cells participate in host defense by surveying for and ultimately killing virally infected or malignant target cells. NK cell cytotoxicity is a tightly regulated process that proceeds stepwise from adhesion and activation to the secretion of preformed lytic granule contents onto a diseased or stressed cell. We previously characterized rapid dynein-dependent lytic granule convergence to the microtubule-organizing center (MTOC) as an early, prerequisite step in NK cell cytotoxicity. Although multiple activating receptors can trigger granule convergence, the specific signal or signals responsible remained unknown. Using live cell confocal microscopy, NK cell lytic granule movement after NK cell activation was captured and measured. Using inhibitors of common early signaling mediators, we show that Src kinases are required for lytic granule convergence, but downstream signals that promote actin rearrangement, MTOC polarization, and calcium mobilization are not. Exposure to interleukin 2 was also sufficient to induce lytic granule convergence, which required noncanonical Src-dependent signaling. Thus, NK cell lytic granule convergence, prompted by specific receptor-mediated and soluble cytokine signals, depends on a directly downstream early Src kinase-dependent signal and emphasizes the importance of this step in readying NK cells for cytotoxicity. (Blood. 2013;121(14):2627-2637) C1 [James, Ashley Mentlik] Univ Penn, Sch Med, Grad Program Cell Biol & Physiol, Philadelphia, PA 19104 USA. [Hsu, Hsiang-Ting; Dongre, Prachi; Mace, Emily M.; Banerjee, Pinaki P.; Orange, Jordan S.] Texas Childrens Hosp, Baylor Coll Med, Ctr Human Immunobiol, Houston, TX 77030 USA. [Uzel, Gulbu] NIAID, NIH, Lab Clin Infect Dis, Bethesda, MD 20892 USA. [Banerjee, Pinaki P.; Orange, Jordan S.] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. RP Orange, JS (reprint author), Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Ctr Human Immunobiol, 1102 Bates Ave,Suite 330, Houston, TX 77030 USA. EM orange@bcm.edu OI orange, jordan/0000-0001-7117-7725 FU National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (NIAID) [AI067946]; NIH NIAID FX This work was supported by National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (NIAID) grant AI067946 (J.S.O.) and an NIH NIAID research supplement to promote diversity in health-related research (A.M.J.). NR 46 TC 17 Z9 18 U1 3 U2 4 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 4 PY 2013 VL 121 IS 14 BP 2627 EP 2637 DI 10.1182/blood-2012-06-437012 PG 11 WC Hematology SC Hematology GA 183NV UT WOS:000321824100011 PM 23380740 ER PT J AU Mace, EM Hsu, AP Monaco-Shawver, L Makedonas, G Rosen, JB Dropulic, L Cohen, JI Frenkel, EP Bagwell, JC Sullivan, JL Biron, CA Spalding, C Zerbe, CS Uzel, G Holland, SM Orange, JS AF Mace, Emily M. Hsu, Amy P. Monaco-Shawver, Linda Makedonas, George Rosen, Joshua B. Dropulic, Lesia Cohen, Jeffrey I. Frenkel, Eugene P. Bagwell, John C. Sullivan, John L. Biron, Christine A. Spalding, Christine Zerbe, Christa S. Uzel, Gulbu Holland, Steven M. Orange, Jordan S. TI Mutations in GATA2 cause human NK cell deficiency with specific loss of the CD56(bright) subset SO BLOOD LA English DT Article ID TRANSCRIPTION FACTOR GATA-2; NATURAL-KILLER-CELLS; HEMATOPOIETIC STEM-CELLS; SPORADIC MONOCYTOPENIA; AUTOSOMAL-DOMINANT; MONOMAC SYNDROME; DISTINCT ROLES; IMMUNODEFICIENCY; MYELODYSPLASIA; DIFFERENTIATE AB Mutations in the transcription factor GATA2 underlie the syndrome of monocytopenia and B-and natural killer (NK)-cell lymphopenia associated with opportunistic infections and cancers. In addition, patients have recurrent and severe viral infections. NK cells play a critical role in mediating antiviral immunity. Human NK cells are thought to mature in a linear fashion, with the CD56(bright) stage preceding terminal maturation to the CD56(dim) stage, considered the most enabled for cytotoxicity. Here we report an NK cell functional defect in GATA2-deficient patients and extend this genetic lesion to what is considered to be the original NK cell-deficient patient. In most cases, GATA2 deficiency is accompanied by a severe reduction in peripheral blood NK cells and marked functional impairment. The NK cells detected in peripheral blood of some GATA2-deficient patients are exclusively of the CD56(dim) subset, which is recapitulated on in vitro NK cell differentiation. In vivo, interferon alpha treatment increased NK cell number and partially restored function but did not correct the paucity of CD56(bright) cells. Thus, GATA2 is required for the maturation of human NK cells and the maintenance of the CD56(bright) pool in the periphery. Defects in GATA2 are a novel cause of profound NK cell dysfunction. (Blood. 2013;121(14):2669-2677) C1 [Mace, Emily M.; Makedonas, George; Orange, Jordan S.] Baylor Coll Med, Houston, TX 77030 USA. [Mace, Emily M.; Makedonas, George; Orange, Jordan S.] Texas Childrens Hosp, Ctr Human Immunobiol, Houston, TX 77030 USA. [Hsu, Amy P.; Spalding, Christine; Zerbe, Christa S.; Uzel, Gulbu; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Monaco-Shawver, Linda; Rosen, Joshua B.] Childrens Hosp Philadelphia, Res Inst, Philadelphia, PA 19104 USA. [Dropulic, Lesia; Cohen, Jeffrey I.] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. [Frenkel, Eugene P.; Bagwell, John C.] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA. [Sullivan, John L.] Univ Massachusetts, Sch Med, Worcester, MA USA. [Biron, Christine A.] Brown Univ, Div Biol & Med, Providence, RI 02912 USA. RP Orange, JS (reprint author), 1102 Bates St,FC330, Houston, TX 77030 USA. EM orange@bcm.edu OI orange, jordan/0000-0001-7117-7725 FU National Institute of Allergy and Infectious Disease [R01 067946]; Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health FX This work was supported by National Institute of Allergy and Infectious Disease grant R01 067946 (J.S.O.) and the Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health. NR 34 TC 53 Z9 55 U1 0 U2 5 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 4 PY 2013 VL 121 IS 14 BP 2669 EP 2677 DI 10.1182/blood-2012-09-453969 PG 9 WC Hematology SC Hematology GA 183NV UT WOS:000321824100015 PM 23365458 ER PT J AU Merling, RK Sweeney, CL Choi, U De Ravin, SS Myers, TG Otaizo-Carrasquero, F Pan, J Linton, G Chen, LF Koontz, S Theobald, NL Malech, HL AF Merling, Randall K. Sweeney, Colin L. Choi, Uimook De Ravin, Suk See Myers, Timothy G. Otaizo-Carrasquero, Francisco Pan, Jason Linton, Gilda Chen, Lifeng Koontz, Sherry Theobald, Narda L. Malech, Harry L. TI Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34(+) cells SO BLOOD LA English DT Article ID PLURIPOTENT STEM-CELLS; CHRONIC GRANULOMATOUS-DISEASE; EPIGENETIC MEMORY; FIBROBLASTS; EXPRESSION AB A variety of somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs), but CD34(+) hematopoietic stem cells (HSCs) present in nonmobilized peripheral blood (PB) would be a convenient target. We report a method for deriving iPSC from PB HSCs using immunobead purification and 2- to 4-day culture to enrich CD34(+) HSCs to 80% +/- 9%, followed by reprogramming with loxP-flanked polycistronic (human Oct4, Klf4, Sox2, and c-Myc) STEMCCA-loxP lentivector, or with Sendai vectors. Colonies arising with STEMCCA-loxP were invariably TRA-1-60(+), yielding 5.3 +/- 2.8 iPSC colonies per 20 mL PB (n = 17), where most colonies had single-copy STEMCCA-loxP easily excised by transient Cre expression. Colonies arising with Sendai were variably reprogrammed (10%-80% TRA-1-60(+)), with variable yield (6 to >500 TRA-1-60(+) iPSC colonies per 10 mL blood; n = 6). Resultant iPSC clones expressed pluripotent cell markers and generated teratomas. Genomic methylation patterns of STEMCCA-loxP-reprogrammed clones closely matched embryonic stem cells. Furthermore, we showed that iPSCs are derived from the nonmobilized CD34(+) HSCs enriched from PB rather than from any lymphocyte or monocyte contaminants because they lack somatic rearrangements typical of T or B lymphocytes and because purified CD14(+) monocytes do not yield iPSC colonies under these reprogramming conditions. (Blood. 2013;121(14):e98-e107) C1 [Merling, Randall K.; Sweeney, Colin L.; Choi, Uimook; De Ravin, Suk See; Pan, Jason; Linton, Gilda; Chen, Lifeng; Koontz, Sherry; Theobald, Narda L.; Malech, Harry L.] NIAID, LHD, NIH, Bethesda, MD 20892 USA. [Myers, Timothy G.; Otaizo-Carrasquero, Francisco] NIAID, Res Technol Branch, NIH, Bethesda, MD 20892 USA. RP Malech, HL (reprint author), NIAID, LHD, NIH, Bldg 10 Room 5-3750,10 Ctr Dr,MSC 1456, Bethesda, MD 20892 USA. EM hmalech@nih.gov OI Malech, Harry/0000-0001-5874-5775 FU Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health; NIH Center for Regenerative Medicine FX All the authors are supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. This work was also supported by an intramural award from the NIH Center for Regenerative Medicine to HLM. NR 18 TC 34 Z9 35 U1 0 U2 5 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 4 PY 2013 VL 121 IS 14 BP E98 EP E107 DI 10.1182/blood-2012-03-420273 PG 10 WC Hematology SC Hematology GA 183NV UT WOS:000321824100001 PM 23386128 ER PT J AU Koutros, S Berndt, SI Barry, KH Andreotti, G Hoppin, JA Sandler, DP Yeager, M Burdett, LA Yuenger, J Alavanja, MCR Freeman, LEB AF Koutros, Stella Berndt, Sonja I. Barry, Kathryn Hughes Andreotti, Gabriella Hoppin, Jane A. Sandler, Dale P. Yeager, Meredith Burdett, Laurie A. Yuenger, Jeffrey Alavanja, Michael C. R. Freeman, Laura E. Beane TI Genetic Susceptibility Loci, Pesticide Exposure and Prostate Cancer Risk SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; AGRICULTURAL HEALTH; SEQUENCE VARIANTS; CHROMOSOME 8Q24; IDENTIFICATION; APPLICATORS; FARMERS; WORKERS; STATES; SOX9 AB Uncovering SNP (single nucleotide polymorphisms)-environment interactions can generate new hypotheses about the function of poorly characterized genetic variants and environmental factors, like pesticides. We evaluated SNP-environment interactions between 30 confirmed prostate cancer susceptibility loci and 45 pesticides and prostate cancer risk in 776 cases and 1,444 controls in the Agricultural Health Study. We used unconditional logistic regression to estimate odds ratios (ORs) and 95% confidence intervals (CIs). Multiplicative SNP-pesticide interactions were calculated using a likelihood ratio test. After correction for multiple tests using the False Discovery Rate method, two interactions remained noteworthy. Among men carrying two T alleles at rs2710647 in EH domain binding protein 1 (EHBP1) SNP, the risk of prostate cancer in those with high malathion use was 3.43 times those with no use (95% CI: 1.44-8.15) (P-interaction = 0.003). Among men carrying two A alleles at rs7679673 in TET2, the risk of prostate cancer associated with high aldrin use was 3.67 times those with no use (95% CI: 1.43, 9.41) (P-interaction = 0.006). In contrast, associations were null for other genotypes. Although additional studies are needed and the exact mechanisms are unknown, this study suggests known genetic susceptibility loci may modify the risk between pesticide use and prostate cancer. C1 [Koutros, Stella; Berndt, Sonja I.; Barry, Kathryn Hughes; Andreotti, Gabriella; Alavanja, Michael C. R.; Freeman, Laura E. Beane] NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Hoppin, Jane A.; Sandler, Dale P.] NIEHS, Epidemiol Branch, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA. [Yeager, Meredith; Burdett, Laurie A.; Yuenger, Jeffrey] NCI, Core Genotyping Facil, Adv Technol Program, Frederick, MD 21701 USA. RP Koutros, S (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. EM KoutrosS@mail.nih.gov RI Beane Freeman, Laura/C-4468-2015; OI Beane Freeman, Laura/0000-0003-1294-4124; Sandler, Dale/0000-0002-6776-0018 FU Intramural Research Program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics [Z01CP010119]; National Institute of Environmental Health Sciences [Z01ES049030] FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics (Z01CP010119) and National Institute of Environmental Health Sciences (Z01ES049030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 13 Z9 13 U1 0 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 4 PY 2013 VL 8 IS 4 AR e58195 DI 10.1371/journal.pone.0058195 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RK UT WOS:000319108100003 PM 23593118 ER PT J AU Kushner, LE Wendelboe, AM Lazzeroni, LC Chary, A Winters, MA Osinusi, A Kottilil, S Polis, MA Holodniy, M AF Kushner, Lauren E. Wendelboe, Aaron M. Lazzeroni, Laura C. Chary, Aarthi Winters, Mark A. Osinusi, Anu Kottilil, Shyam Polis, Michael A. Holodniy, Mark TI Immune Biomarker Differences and Changes Comparing HCV Mono-Infected, HIV/HCV Co-Infected, and HCV Spontaneously Cleared Patients SO PLOS ONE LA English DT Article ID CHRONIC HEPATITIS-C; HUMAN-IMMUNODEFICIENCY-VIRUS; HIV-INFECTION; CARDIOVASCULAR-DISEASE; MULTIPLE COMPARISONS; COINFECTED PATIENTS; EPIDEMIOLOGIC DATA; INTERFERON-ALPHA; LIVER-DISEASE; PATHOGENESIS AB Background: Immune biomarkers are implicated in HCV treatment response, fibrosis, and accelerated pathogenesis of comorbidities, though only D-dimer and C-reactive protein have been consistently studied. Few studies have evaluated HIV/HCV co-infection, and little longitudinal data exists describing a broader antiviral cytokine response Methods: Fifty immune biomarkers were analyzed at baseline(BL) and HCV end of treatment follow-up(FU) time point using the Luminex 50-plex assay in plasma samples from 15 HCV-cleared, 24 HCV mono- and 49 HIV/HCV co-infected patients receiving antiretroviral treatment, who either did or did not receive pegylated-interferon/ribavirin HCV treatment. Biomarker levels were compared among spontaneous clearance patients, mono- and co-infected, untreated and HCV-treated, and sustained virologic responders (SVR) and non-responders (NR) at BL and FU using nonparametric analyses. A Bonferroni correction, adjusting for tests of 50 biomarkers, was used to reduce Type I error Results: Compared to HCV patients at BL, HIV/HCV patients had 22 significantly higher and 4 significantly lower biomarker levels, following correction for multiple testing. There were no significantly different BL levels when comparing SVR and NR in mono- or co-infected patients; however, FU levels changed considerably in co-infected patients, with seven becoming significantly higher and eight becoming significantly lower in SVR patients. Longitudinally between BL and FU, 13 markers significantly changed in co-infected SVR patients, while none significantly changed in co-infected NR patients. There were also no significant changes in longitudinal analyses of mono-infected patients achieving SVR or mono-infected and co-infected groups deferring treatment Conclusions: Clear differences exist in pattern and quantity of plasma immune biomarkers among HCV mono-infected, HIV/HCV co-infected, and HCV-cleared patients; and with SVR in co-infected patients treated for HCV. Though >90% of patients were male and co-infected had a larger percentage of African American patients, our findings may have implications for better understanding HCV pathogenesis, treatment outcomes, and future therapeutic targets C1 [Kushner, Lauren E.; Chary, Aarthi; Winters, Mark A.; Holodniy, Mark] Vet Affairs Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA. [Wendelboe, Aaron M.] Univ Oklahoma, Oklahoma City, OK USA. [Lazzeroni, Laura C.; Chary, Aarthi; Winters, Mark A.; Holodniy, Mark] Stanford Univ, Stanford, CA 94305 USA. [Osinusi, Anu; Kottilil, Shyam; Polis, Michael A.] NIAID, Immunoregulat Lab, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Holodniy, M (reprint author), Vet Affairs Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA. EM mark.holodniy@va.gov OI Lazzeroni, Laura/0000-0002-1846-6920; Polis, Michael/0000-0002-9151-2268 FU Department of Veterans Affairs [HOM0057]; National Cancer Institute, National Institutes of Health (NIH) [HHSN261200800001E]; Intramural Research Program of the NIH (National Institute of Allergy and Infectious Diseases); Clinical Science Research & Development Service of the Department of Veterans Affairs (A Cooperative Studies Program-Wide DNA Bank, CSP) [478] FX This study was funded by a grant to MH from the Department of Veterans Affairs(Grant HOM0057 for "Evaluation of Viral and Immune Responses to Treatment in People with HIV or HCV Infections''). This project was also funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health (NIH), under Contract No. HHSN261200800001E. This research was supported in whole or in part by the Intramural Research Program of the NIH (National Institute of Allergy and Infectious Diseases) and by the Clinical Science Research & Development Service of the Department of Veterans Affairs (A Cooperative Studies Program-Wide DNA Bank, CSP#478). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 7 Z9 7 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 4 PY 2013 VL 8 IS 4 AR e60387 DI 10.1371/journal.pone.0060387 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RK UT WOS:000319108100036 PM 23593207 ER PT J AU van der Loos, MJHM Rietveld, CA Eklund, N Koellinger, PD Rivadeneira, F Abecasis, GR Ankra-Badu, GA Baumeister, SE Benjamin, DJ Biffar, R Blankenberg, S Boomsma, DI Cesarini, D Cucca, F de Geus, EJC Dedoussis, G Deloukas, P Dimitriou, M Eiriksdottir, G Eriksson, J Gieger, C Gudnason, V Hohne, B Holle, R Hottenga, JJ Isaacs, A Jarvelin, MR Johannesson, M Kaakinen, M Kahonen, M Kanoni, S Laaksonen, MA Lahti, J Launer, LJ Lehtimaki, T Loitfelder, M Magnusson, PKE Naitza, S Oostra, BA Perola, M Petrovic, K Quaye, L Raitakari, O Ripatti, S Scheet, P Schlessinger, D Schmidt, CO Schmidt, H Schmidt, R Senft, A Smith, AV Spector, TD Surakka, I Svento, R Terracciano, A Tikkanen, E van Duijn, CM Viikari, J Volzke, H Wichmann, HE Wild, PS Willems, SM Willemsen, G van Rooij, FJA Groenen, PJF Uitterlinden, AG Hofman, A Thurik, AR AF van der Loos, Matthijs J. H. M. Rietveld, Cornelius A. Eklund, Niina Koellinger, Philipp D. Rivadeneira, Fernando Abecasis, Goncalo R. Ankra-Badu, Georgina A. Baumeister, Sebastian E. Benjamin, Daniel J. Biffar, Reiner Blankenberg, Stefan Boomsma, Dorret I. Cesarini, David Cucca, Francesco de Geus, Eco J. C. Dedoussis, George Deloukas, Panos Dimitriou, Maria Eiriksdottir, Guony Eriksson, Johan Gieger, Christian Gudnason, Vilmundur Hoehne, Birgit Holle, Rolf Hottenga, Jouke-Jan Isaacs, Aaron Jarvelin, Marjo-Riitta Johannesson, Magnus Kaakinen, Marika Kahonen, Mika Kanoni, Stavroula Laaksonen, Maarit A. Lahti, Jari Launer, Lenore J. Lehtimaki, Terho Loitfelder, Marisa Magnusson, Patrik K. E. Naitza, Silvia Oostra, Ben A. Perola, Markus Petrovic, Katja Quaye, Lydia Raitakari, Olli Ripatti, Samuli Scheet, Paul Schlessinger, David Schmidt, Carsten O. Schmidt, Helena Schmidt, Reinhold Senft, Andrea Smith, Albert V. Spector, Timothy D. Surakka, Ida Svento, Rauli Terracciano, Antonio Tikkanen, Emmi van Duijn, Cornelia M. Viikari, Jorma Voelzke, Henry Wichmann, H. -Erich Wild, Philipp S. Willems, Sara M. Willemsen, Gonneke van Rooij, Frank J. A. Groenen, Patrick J. F. Uitterlinden, Andre G. Hofman, Albert Thurik, A. Roy TI The Molecular Genetic Architecture of Self-Employment SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; CORONARY HEART-DISEASE; COMMON SNPS EXPLAIN; CARDIOVASCULAR-DISEASE; SOCIOECONOMIC-STATUS; EDUCATIONAL-ATTAINMENT; MISSING HERITABILITY; LARGE PROPORTION; RISK-FACTORS; HUMAN HEIGHT AB Economic variables such as income, education, and occupation are known to affect mortality and morbidity, such as cardiovascular disease, and have also been shown to be partly heritable. However, very little is known about which genes influence economic variables, although these genes may have both a direct and an indirect effect on health. We report results from the first large-scale collaboration that studies the molecular genetic architecture of an economic variable-entrepreneurship-that was operationalized using self-employment, a widely-available proxy. Our results suggest that common SNPs when considered jointly explain about half of the narrow-sense heritability of self-employment estimated in twin data (sigma(2)(g)/sigma(2)(P) = 25%, h(2) = 55%). However, a meta-analysis of genome-wide association studies across sixteen studies comprising 50,627 participants did not identify genome-wide significant SNPs. 58 SNPs with p<10(-5) were tested in a replication sample (n = 3,271), but none replicated. Furthermore, a gene-based test shows that none of the genes that were previously suggested in the literature to influence entrepreneurship reveal significant associations. Finally, SNP-based genetic scores that use results from the meta-analysis capture less than 0.2% of the variance in self-employment in an independent sample (p >= 0.039). Our results are consistent with a highly polygenic molecular genetic architecture of self-employment, with many genetic variants of small effect. Although self-employment is a multi-faceted, heavily environmentally influenced, and biologically distal trait, our results are similar to those for other genetically complex and biologically more proximate outcomes, such as height, intelligence, personality, and several diseases. C1 [van der Loos, Matthijs J. H. M.; Rietveld, Cornelius A.; Koellinger, Philipp D.; Thurik, A. Roy] Erasmus Univ, Erasmus Sch Econ, Dept Appl Econ, Rotterdam, Netherlands. [van der Loos, Matthijs J. H. M.; Rietveld, Cornelius A.; Koellinger, Philipp D.; Rivadeneira, Fernando; van Rooij, Frank J. A.; Uitterlinden, Andre G.; Hofman, Albert] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Eklund, Niina; Perola, Markus; Ripatti, Samuli; Surakka, Ida; Tikkanen, Emmi] Univ Helsinki, Inst Mol Med Finland FIMM, Helsinki, Finland. [Eklund, Niina; Perola, Markus; Ripatti, Samuli; Surakka, Ida; Tikkanen, Emmi] Natl Inst Hlth & Welf THL, Dept Chron Dis Prevent, Publ Hlth Genom Unit, Helsinki, Finland. [Rivadeneira, Fernando; Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Abecasis, Goncalo R.] Univ Michigan, Sch Publ Hlth, Dept Biostat, Ctr Stat Genet, Ann Arbor, MI 48109 USA. [Ankra-Badu, Georgina A.; Quaye, Lydia; Spector, Timothy D.] Kings Coll London, Dept Twin Res & Genet Epidemiol, London WC2R 2LS, England. [Baumeister, Sebastian E.; Schmidt, Carsten O.; Voelzke, Henry] Univ Med Greifswald, Inst Community Med, Greifswald, Germany. [Benjamin, Daniel J.] Cornell Univ, Dept Econ, Ithaca, NY 14853 USA. [Biffar, Reiner] Ernst Moritz Arndt Univ Greifswald, Ctr Oral Hlth, Dept Prosthet Dent Gerodontol & Biomat, Greifswald, Germany. [Blankenberg, Stefan] Univ Med Ctr Hamburg Eppendorf, Dept Gen & Intervent Cardiol, Hamburg, Germany. [Boomsma, Dorret I.; de Geus, Eco J. C.; Hottenga, Jouke-Jan; Willemsen, Gonneke] Vrije Univ Amsterdam, Dept Biol Psychol, Netherlands Twin Register, Amsterdam, Netherlands. [Cesarini, David] NYU, Dept Econ, Ctr Expt Social Sci, New York, NY 10003 USA. [Cucca, Francesco; Naitza, Silvia] CNR, Ist Ric Genet & Biomed, Cagliari, Italy. [Dedoussis, George; Dimitriou, Maria] Harokopio Univ Athens, Dept Nutr & Dietet, Athens, Greece. [Deloukas, Panos; Kanoni, Stavroula; Ripatti, Samuli] Wellcome Trust Sanger Inst, Hinxton, England. [Eiriksdottir, Guony; Gudnason, Vilmundur; Smith, Albert V.] Iceland Heart Assoc, Kopavogur, Iceland. [Eriksson, Johan] Natl Inst Hlth & Welf THL, Dept Chron Dis Prevent, Diabet Prevent Unit, Helsinki, Finland. [Eriksson, Johan] Univ Helsinki, Dept Gen Practice & Primary Hlth Care, Helsinki, Finland. [Eriksson, Johan] Folkhalsan Res Ctr, Helsinki, Finland. [Eriksson, Johan] Univ Helsinki, Cent Hosp, Unit Gen Practice, Helsinki, Finland. [Eriksson, Johan] Vaasa Cent Hosp, Vaasa, Finland. [Gieger, Christian; Hoehne, Birgit] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Genet Epidemiol, Neuherberg, Germany. [Gudnason, Vilmundur; Smith, Albert V.] Univ Iceland, Dept Med, Reykjavik, Iceland. [Hoehne, Birgit] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Epidemiol 2, Neuherberg, Germany. [Holle, Rolf] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Hlth Econ & Hlth Care Management, Neuherberg, Germany. [Isaacs, Aaron; van Duijn, Cornelia M.; Willems, Sara M.] Erasmus MC, Dept Epidemiol, Genet Epidemiol Unit, Rotterdam, Netherlands. [Isaacs, Aaron; van Duijn, Cornelia M.] Ctr Med Syst Biol, Leiden, Netherlands. [Jarvelin, Marjo-Riitta; Kaakinen, Marika] Univ Oulu, Inst Hlth Sci, Oulu, Finland. [Jarvelin, Marjo-Riitta; Kaakinen, Marika] Univ Oulu, Bioctr, Oulu, Finland. [Jarvelin, Marjo-Riitta] Natl Inst Hlth & Welf, Dept Life Course & Serv, Oulu, Finland. [Jarvelin, Marjo-Riitta] Univ London Imperial Coll Sci Technol & Med, MRC HPA Ctr Environm & Hlth, Dept Epidemiol & Biostat, London, England. [Johannesson, Magnus] Stockholm Sch Econ, Dept Econ, S-11383 Stockholm, Sweden. [Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere, Finland. [Kahonen, Mika] Univ Tampere, Sch Med, Dept Clin Physiol, FIN-33101 Tampere, Finland. [Laaksonen, Maarit A.] Natl Inst Hlth & Welf THL, Dept Hlth Funct Capac & Welf, Populat Hlth Res Unit, Helsinki, Finland. [Lahti, Jari] Univ Helsinki, Inst Behav Sci, Helsinki, Finland. [Launer, Lenore J.] NIA, Lab Epidemiol Demog & Biometry, Intramural Res Program, Bethesda, MD 20892 USA. [Lehtimaki, Terho] Tampere Univ Hosp, Dept Clin Chem, Fimlab Labs, FIN-33521 Tampere, Finland. [Lehtimaki, Terho] Univ Tampere, Sch Med, Dept Clin Chem, FIN-33101 Tampere, Finland. [Loitfelder, Marisa; Schmidt, Reinhold] Med Univ Graz, Div Neurogeriatr, Dept Neurol, Graz, Austria. [Magnusson, Patrik K. E.] Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden. [Oostra, Ben A.] Erasmus MC, Dept Clin Genet, Rotterdam, Netherlands. [Perola, Markus] Univ Tartu, Estonian Genome Ctr, EE-50090 Tartu, Estonia. [Petrovic, Katja] Gen Hosp, Dept Neurol, Div Gen Neurol, Graz, Austria. [Petrovic, Katja] Med Univ Graz, Graz, Austria. [Raitakari, Olli] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, FIN-20520 Turku, Finland. [Raitakari, Olli] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Scheet, Paul] Univ Texas MD Anderson Canc Ctr, Dept Epidemiol, Houston, TX 77030 USA. [Schlessinger, David; Terracciano, Antonio] NIA, NIH, Baltimore, MD 21224 USA. [Schmidt, Helena] Med Univ Graz, Inst Mol Biol & Biochem, Graz, Austria. [Senft, Andrea] Med Univ Lubeck, Univ Klinikum Schleswig Holstein, Inst Med Biometrie & Stat, D-23538 Lubeck, Germany. [Svento, Rauli] Univ Oulu, Oulu Business Sch, Dept Econ, Oulu, Finland. [Terracciano, Antonio] Florida State Univ, Coll Med, Tallahassee, FL 32306 USA. [Viikari, Jorma] Turku Univ Hosp, Dept Med, FIN-20520 Turku, Finland. [Viikari, Jorma] Univ Turku, Dept Med, Turku, Finland. [Wichmann, H. -Erich] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Epidemiol 1, Neuherberg, Germany. [Wichmann, H. -Erich] Univ Munich, Chair Epidemiol, Inst Med Informat Biometry & Epidemiol, Munich, Germany. [Wichmann, H. -Erich] Univ Munich, Klinikum Grosshadern, D-80539 Munich, Germany. [Wild, Philipp S.] Johannes Gutenberg Univ Mainz, Univ Med Ctr Mainz, Ctr Thrombosis & Hemostasis, D-55122 Mainz, Germany. [Wild, Philipp S.] Johannes Gutenberg Univ Mainz, Univ Med Ctr Mainz, Dept Med 2, D-55122 Mainz, Germany. [Groenen, Patrick J. F.] Erasmus Univ, Erasmus Sch Econ, Inst Econometr, Rotterdam, Netherlands. [Thurik, A. Roy] Panteia, Zoetermeer, Netherlands. [Thurik, A. Roy] GSCM Montpellier Business Sch, Montpellier, France. RP van der Loos, MJHM (reprint author), Erasmus Univ, Erasmus Sch Econ, Dept Appl Econ, Rotterdam, Netherlands. EM mvanderloos@ese.eur.nl RI Magnusson, Patrik/C-4458-2017; Naitza, Silvia/D-5620-2017; Johannesson, Magnus/E-9680-2011; terracciano, antonio/B-1884-2008; Deloukas, Panos/B-2922-2013; Thurik, Roy/A-9386-2012; Gudnason, Vilmundur/K-6885-2015; Ripatti, Samuli/H-9446-2014; de Geus, Eco/M-9318-2015; Rivadeneira, Fernando/O-5385-2015; Smith, Albert/K-5150-2015; Holle, Rolf/D-9333-2013; OI Lahti, Jari/0000-0002-4310-5297; Koellinger, Philipp/0000-0001-7413-0412; Cesarini, David/0000-0002-0043-009X; Abecasis, Goncalo/0000-0003-1509-1825; Gieger, Christian/0000-0001-6986-9554; Jarvelin, Marjo-Riitta/0000-0002-2149-0630; Groenen, Patrick/0000-0001-6683-8971; Eriksson, Johan/0000-0002-2516-2060; Johannesson, Magnus/0000-0001-8759-6393; Deloukas, Panos/0000-0001-9251-070X; Thurik, Roy/0000-0002-0242-6908; Gudnason, Vilmundur/0000-0001-5696-0084; Ripatti, Samuli/0000-0002-0504-1202; de Geus, Eco/0000-0001-6022-2666; Rivadeneira, Fernando/0000-0001-9435-9441; Smith, Albert/0000-0003-1942-5845; Holle, Rolf/0000-0001-5395-2695; Kaakinen, Marika/0000-0002-9228-0462 FU National Institutes of Health [N01-AG-12100]; NIA Intramural Research Program, Hjartavernd (the Icelandic Heart Association); Althingi (the Icelandic Parliament); Austrian Science Fond (FWF) [P20545-P05, P13180]; EUROSPAN (European Special Populations Research Network); European Commission [018947, LSHG-CT-2006-01947, QLG1-CT-2000-01643]; Netherlands Organization for Scientific Research, Erasmus MC; Centre for Medical Systems Biology (CMSB); Netherlands Brain Foundation (Hersenstichting Nederland); government of Rheinland-Pfalz ("Stiftung Rheinland Pfalz fur Innovation") [AZ 961-386261/733]; Johannes Gutenberg-University of Mainz; Boehringer Ingelheim; Philips Medical Systems; Gutenberg Health Study [H2000]; budgetary funds of National Institute for Health and Welfare (THL); Finnish Centre for Pensions (ETK); Social Insurance Institution of Finland (KELA); Local Government Pensions Institution (KEVA); Academy of Finland [120315, 129287, 1129457, 1216965, 120386, 125876, 104781, 129418, 126925, 121584, 124282, 129378, 117787, 41071]; Finnish Diabetes Research Society; Folkhalsan Research Foundation; Novo Nordisk Foundation; Finska Lakaresallskapet; European Science Foundation (EuroSTRESS); Wellcome Trust [89061/Z/09/Z, 089062/Z/09/Z]; Samfundet Folkhalsan; Signe and Ane Gyllenberg foundation; Finnish Academy SALVE program "Pubgensense" [129322]; National Institute on Aging [NIA U01AG009740, NO1-AG-1-2109]; Helmholtz Zentrum Munchen; German Research Center for Environmental Health, Neuherberg, Germany; German Federal Ministry of Education and Research (BMBF); German National Genome Research Network (NGFN); Munich Center of Health Sciences (MC Health) as part of LMUinnovativ [NFBC1966]; Center of Excellence in Complex Disease Genetics and SALVE; University Hospital Oulu, Biocenter, University of Oulu, Finland [75617]; NHLBI through the STAMPEED program [5R01HL087679-02, 1RL1MH083268-01]; NIH/NIMH [5R01MH63706:02]; ENGAGE project [HEALTH-F4-2007-201413]; Medical Research Council, UK [G0500539, G0600705]; Netherlands Organization for Scientific Research (NWO) [904-61-090, 985-10-002, 904-61-193, 480-04-004, 400-05-717, Addiction-31160008 Middelgroot-911-09-032, Spinozapremie 56-46414192]; Center for Medical Systems Biology (CSMB, NWO Genomics); NBIC/BioAssist/RK [2008.024]; Biobanking and Biomolecular Resources Research Infrastructure (BBMRI-NL) [184.021.007]; VU University's Institute for Health and Care Research (EMGO+); Neuroscience Campus Amsterdam (NCA); European Science Foundation (ESF) [EU/QLRT-2001-01254]; European Community; ENGAGE [HEALTH-F4-2007-201413]; European Science Council (ERC) [230374]; Rutgers University Cell and DNA Repository [NIMH U24 MH068457-06]; Avera Institute, Sioux Falls, South Dakota (USA); National Institutes of Health (NIH) [R01D0042157-01A]; Genetic Association Information Network (GAIN) of the Foundation for the US National Institutes of Health; NIMH [MH081802]; Grand Opportunity from the NIMH [1RC2MH089951-01, 1RC2 MH089995-01]; Erasmus Medical Center and Erasmus University, Rotterdam; Netherlands Organization for the Health Research and Development (ZonMw); Research Institute for Diseases in the Elderly (RIDE); Ministry of Education, Culture and Science; Ministry for Health, Welfare and Sports; Municipality of Rotterdam; Netherlands Organisation for Scientific Research NWO Investments [175.010.2005.011, 911-03-012]; Research Institute for Diseases in the Elderly [014-93-015]; Netherlands Genomics Initiative (NGI)/Netherlands Consortium for Healthy Aging (NCHA) [050-060-810]; Netherlands Scientific Organization [NWO 480-05-003]; Intramural Research Program of the National Institute on Aging, NIH; Federal Ministry of Education and Research [BMBF 01ZZ9603, 01ZZ0103, 03IS2061A, 03ZIK012]; Ministry of Cultural Affairs as well as the Social Ministry of the Federal State of Mecklenburg-West Pomerania; Siemens Healthcare, Erlangen, Germany; Federal State of Mecklenburg-West Pomerania; Swedish Council for Working Life and Social Research; Jan Wallander and Tom Hedelius Foundation; Ragnar Soderberg Foundation; Ministry for Higher Education; Swedish Research Council [M-2005-1112]; GenomEUtwin [EU/QLRT-2001-01254, QLG2-CT-2002-01254]; NIH [DK U01-066134]; Swedish Foundation for Strategic Research (SSF); Heart and Lung foundation [20070481]; Greek General Secretary of Research and Technology [PENED 2003]; Wellcome Trust Sanger Institute; Wellcome Trust; Dept of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre award; National Eye Institute via an NIH/CIDR genotyping project; Social Insurance Institution of Finland, Kuopio, Tampere and Turku University Hospital Medical Funds [9M048]; Juho Vainio Foundation; Paavo Nurmi Foundation; Finnish Foundation of Cardiovascular Research and Finnish Cultural Foundation; Tampere Tuberculosis Foundation; Emil Aaltonen Foundation FX AGES: The AGES-Reykjavik Study is funded by National Institutes of Health contract N01-AG-12100, the NIA Intramural Research Program, Hjartavernd (the Icelandic Heart Association), and the Althingi (the Icelandic Parliament); ASPS: The research reported in this article was funded by the Austrian Science Fond (FWF) grant number P20545-P05 and P13180. The Medical University of Graz supports the databank of the ASPS; ERF: The genotyping for the ERF study was supported by EUROSPAN (European Special Populations Research Network) and the European Commission FP6 STRP grant (018947; LSHG-CT-2006-01947). The ERF study was further supported by grants from the Netherlands Organization for Scientific Research, Erasmus MC, the Centre for Medical Systems Biology (CMSB) and the Netherlands Brain Foundation (Hersenstichting Nederland); GHS: This work/the Gutenberg Health Study is funded through the government of Rheinland-Pfalz ("Stiftung Rheinland Pfalz fur Innovation'', contract number AZ 961-386261/733), the research programs "Wissen schafft Zukunft'' and "Schwerpunkt Vaskulare Pravention'' of the Johannes Gutenberg-University of Mainz and its contract with Boehringer Ingelheim and Philips Medical Systems including an unrestricted grant for the Gutenberg Health Study; H2000: The study was funded mainly by the budgetary funds of National Institute for Health and Welfare (THL). The Finnish Centre for Pensions (ETK), the Social Insurance Institution of Finland (KELA), the Local Government Pensions Institution (KEVA) and other organisations listed on the website of the survey (http://www.terveys2000.fi) also contributed to funding; HBCS: The Helsinki Birth Cohort Study has been supported by grants from the Academy of Finland (Grant No. 120315 and 129287 to EW, 1129457 and 1216965 to KR, 120386 and 125876 to JGE), the Finnish Diabetes Research Society, Folkhalsan Research Foundation, Novo Nordisk Foundation, Finska Lakaresallskapet, the European Science Foundation (EuroSTRESS), the Wellcome Trust (Grant No. 89061/Z/09/Z and 089062/Z/09/Z), Samfundet Folkhalsan, Finska Lakaresallskapet and the Signe and Ane Gyllenberg foundation. Markus Perola is partly financially supported for this work by the Finnish Academy SALVE program "Pubgensense'' 129322; HRS: The Health and Retirement Study is sponsored by the National Institute on Aging (grant number NIA U01AG009740) and is conducted by the University of Michigan; KORA S4: The KORA Augsburg studies were financed by the Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Neuherberg, Germany and supported by grants from the German Federal Ministry of Education and Research (BMBF). Part of this work was financed by the German National Genome Research Network (NGFN).; Our research was supported within the Munich Center of Health Sciences (MC Health) as part of LMUinnovativ; NFBC1966: Academy of Finland [project grants 104781, 120315, 129418 and Center of Excellence in Complex Disease Genetics and SALVE], University Hospital Oulu, Biocenter, University of Oulu, Finland (75617), the European Commission [EURO-BLCS, Framework 5 award QLG1-CT-2000-01643], NHLBI [5R01HL087679-02] through the STAMPEED program [1RL1MH083268-01], NIH/NIMH [5R01MH63706:02], ENGAGE project and grant agreement [HEALTH-F4-2007-201413], and the Medical Research Council, UK [G0500539, G0600705, PrevMetSyn/SALVE]; NTR: The Netherlands Twin Register data collection and genotyping has been funded by the Netherlands Organization for Scientific Research (NWO: MagW/ZonMW grants 904-61-090, 985-10-002,904-61-193,480-04-004, 400-05-717, Addiction-31160008 Middelgroot-911-09-032, Spinozapremie 56-46414192), Center for Medical Systems Biology (CSMB, NWO Genomics), NBIC/BioAssist/RK(2008.024), Biobanking and Biomolecular Resources Research Infrastructure (BBMRI-NL, 184.021.007), the VU University's Institute for Health and Care Research (EMGO+) and Neuroscience Campus Amsterdam (NCA), the European Science Foundation (ESF, EU/QLRT-2001-01254), the European Community's Seventh Framework Program (FP7/2007-2013), ENGAGE (HEALTH-F4-2007-201413); the European Science Council (ERC Advanced, 230374), Rutgers University Cell and DNA Repository (NIMH U24 MH068457-06), the Avera Institute, Sioux Falls, South Dakota (USA) and the National Institutes of Health (NIH, R01D0042157-01A). Part of the genotyping and analyses were funded by the Genetic Association Information Network (GAIN) of the Foundation for the US National Institutes of Health, the (NIMH, MH081802) and by the Grand Opportunity grants 1RC2MH089951-01 and 1RC2 MH089995-01 from the NIMH; RS: The Rotterdam Study is funded by the Erasmus Medical Center and Erasmus University, Rotterdam, the Netherlands Organization for the Health Research and Development (ZonMw), the Research Institute for Diseases in the Elderly (RIDE), the Ministry of Education, Culture and Science, the Ministry for Health, Welfare and Sports, the European Commission (DG XII), and the Municipality of Rotterdam. The GWAS database of the Rotterdam Study was funded by the Netherlands Organisation for Scientific Research NWO Investments (nr. 175.010.2005.011, 911-03-012), the Research Institute for Diseases in the Elderly (014-93-015; RIDE2), and the Netherlands Genomics Initiative (NGI)/Netherlands Consortium for Healthy Aging (NCHA) project nr. 050-060-810. Statistical analyses were partly carried out on the Genetic Cluster Computer (http://www.geneticcluster.org) which is financially supported by the Netherlands Scientific Organization (NWO 480-05-003) along with a supplement from the Dutch Brain Foundation and the VU University Amsterdam; SardiNIA: This research was supported in part by the Intramural Research Program of the National Institute on Aging, NIH, and by the National Institute on Aging contract NO1-AG-1-2109 to the SardiNIA/ProgeNIA team; SHIP: SHIP is part of the Community Medicine Research net (www.community-medicine.de) and the Greifswald Approach to Individualized Medicine (GANI_MED) consortium (www.gani-med.de) of the University Medicine Greifswald, Germany, which are funded by the Federal Ministry of Education and Research (BMBF 01ZZ9603, 01ZZ0103 and 03IS2061A), the Ministry of Cultural Affairs as well as the Social Ministry of the Federal State of Mecklenburg-West Pomerania.; Genome-wide data have been supported by the Federal Ministry of Education and Research (grant no. 03ZIK012) and a joint grant from Siemens Healthcare, Erlangen, Germany and the Federal State of Mecklenburg-West Pomerania. The University of Greifswald is a member of the 'Center of Knowledge Interchange' program of the Siemens AG and the Cache Campus program of the InterSystems GmbH; STR: Financial support was received from The Swedish Council for Working Life and Social Research, The Jan Wallander and Tom Hedelius Foundation, the Ragnar Soderberg Foundation, the Ministry for Higher Education, the Swedish Research Council (M-2005-1112), GenomEUtwin (EU/QLRT-2001-01254; QLG2-CT-2002-01254), NIH DK U01-066134, The Swedish Foundation for Strategic Research (SSF), and the Heart and Lung foundation no. 20070481; THISEAS: Recruitment for THISEAS was partially funded by a research grant (PENED 2003) from the Greek General Secretary of Research and Technology. Genotyping was supported by the Wellcome Trust Sanger Institute; TwinsUK: The study was funded by the Wellcome Trust, the European Community's Seventh Framework Programme (FP7/2007-2013), and the ENGAGE project grant agreement (HEALTH-F4-2007-201413). The study also receives support from the Dept of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre award to Guy's & St Thomas' NHS Foundation Trust in partnership with King's College London. TDS is an NIHR senior Investigator and is holder of an ERC Advanced Principal Investigator award. Genotyping was performed by The Wellcome Trust Sanger Institute, support of the National Eye Institute via an NIH/CIDR genotyping project; YFS: The Young Finns Study has been financially supported by the Academy of Finland: grants 126925, 121584, 124282, 129378 (Salve), 117787 (Gendi), and 41071 (Skidi), the Social Insurance Institution of Finland, Kuopio, Tampere and Turku University Hospital Medical Funds (grant 9M048 for TeLeht), Juho Vainio Foundation, Paavo Nurmi Foundation, Finnish Foundation of Cardiovascular Research and Finnish Cultural Foundation, Tampere Tuberculosis Foundation and Emil Aaltonen Foundation (TL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 105 TC 12 Z9 12 U1 6 U2 49 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 4 PY 2013 VL 8 IS 4 AR e60542 DI 10.1371/journal.pone.0060542 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 146RK UT WOS:000319108100052 PM 23593239 ER PT J AU Wu, D Minton, AP AF Wu, Di Minton, Allen P. TI Compensating Effects of Urea and Trimethylamine-N-Oxide on the Heteroassociation of alpha-Chymotrypsin and Soybean Trypsin Inhibitor SO JOURNAL OF PHYSICAL CHEMISTRY B LA English DT Article ID STATIC LIGHT-SCATTERING; QUANTITATIVE CHARACTERIZATION; VOLUME EXCLUSION; PROTEIN; COUNTERACTION; DISSOCIATION; DIMERIZATION; KINETICS; KUNITZ AB An assay for the determination of the equilibrium constant for heteroassociation of alpha-chymotrypsin and soybean trypsin inhibitor via fluorescence depolarization is described. Results obtained at neutral pH in saline buffer were consistent with prior determinations via sedimentation equilibrium and static light scattering. The dependence of the association equilibrium constant upon the concentrations of urea and trimethylamine-N-oxide (TMAO) added individually and in mixtures was determined at several temperatures. It was found that subdenaturing concentrations of urea decrease the extent of heteroassociation and that added TMAO increases the extent of heteroassociation. The effects of both cosolutes in mixtures upon the equilibrium heteroassociation of alpha-chymotrypsin and soybean trypsin inhibitor appear to be additive. A thermodynamic analysis of the combined results is presented. C1 [Wu, Di; Minton, Allen P.] NIDDK, Sect Phys Biochem, Lab Biochem & Genet, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Minton, AP (reprint author), NIDDK, Sect Phys Biochem, Lab Biochem & Genet, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA. EM minton@helix.nih.gov FU Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases FX This research has been supported by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases. The authors thank Dr. Peter McPhie, NIH, for critically reviewing a draft of this report. NR 26 TC 4 Z9 4 U1 4 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1520-6106 J9 J PHYS CHEM B JI J. Phys. Chem. B PD APR 4 PY 2013 VL 117 IS 13 BP 3554 EP 3559 DI 10.1021/jp4006923 PG 6 WC Chemistry, Physical SC Chemistry GA 122KQ UT WOS:000317317400013 PM 23472887 ER PT J AU Yutin, N Colson, P Raoult, D Koonin, EV AF Yutin, Natalya Colson, Philippe Raoult, Didier Koonin, Eugene V. TI Mimiviridae: clusters of orthologous genes, reconstruction of gene repertoire evolution and proposed expansion of the giant virus family SO VIROLOGY JOURNAL LA English DT Article ID LARGE DNA VIRUSES; VIROPHAGE; LIKELIHOOD; AMEBAS; ORIGIN; MICROORGANISMS; PHAEOCYSTIS; EUKARYOTES; HIGHLIGHTS; PARSIMONY AB Background: The family Mimiviridae belongs to the large monophyletic group of Nucleo-Cytoplasmic Large DNA Viruses (NCLDV; proposed order Megavirales) and encompasses giant viruses infecting amoeba and probably other unicellular eukaryotes. The recent discovery of the Cafeteria roenbergensis virus (CroV), a distant relative of the prototype mimiviruses, led to a substantial expansion of the genetic variance within the family Mimiviridae. In the light of these findings, a reassessment of the relationships between the mimiviruses and other NCLDV and reconstruction of the evolution of giant virus genomes emerge as interesting and timely goals. Results: Database searches for the protein sequences encoded in the genomes of several viruses originally classified as members of the family Phycodnaviridae, in particular Organic Lake phycodnaviruses and Phaeocystis globosa viruses (OLPG), revealed a greater number of highly similar homologs in members of the Mimiviridae than in phycodnaviruses. We constructed a collection of 898 Clusters of Orthologous Genes for the putative expanded family Mimiviridae (MimiCOGs) and used these clusters for a comprehensive phylogenetic analysis of the genes that are conserved in most of the NCLDV. The topologies of the phylogenetic trees for these conserved viral genes strongly support the monophyly of the OLPG and the mimiviruses. The same tree topology was obtained by analysis of the phyletic patterns of conserved viral genes. We further employed the mimiCOGs to obtain a maximum likelihood reconstruction of the history of genes losses and gains among the giant viruses. The results reveal massive gene gain in the mimivirus branch and modest gene gain in the OLPG branch. Conclusions: These phylogenomic results reported here suggest a substantial expansion of the family Mimiviridae. The proposed expanded family encompasses a greater diversity of viruses including a group of viruses with much smaller genomes than those of the original members of the Mimiviridae. If the OLPG group is included in an expanded family Mimiviridae, it becomes the only family of giant viruses currently shown to host virophages. The mimiCOGs are expected to become a key resource for phylogenomics of giant viruses. C1 [Yutin, Natalya; Koonin, Eugene V.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. [Colson, Philippe; Raoult, Didier] Aix Marseille Univ, Fac Med, URMITE, UM 63,CNRS 7278,IRD 198,INSERM,U1095, F-13385 Marseille 5, France. RP Koonin, EV (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. EM koonin@ncbi.nlm.nih.gov RI Colson, Philippe/P-6355-2014 OI Colson, Philippe/0000-0001-6285-0308 FU US Department of Health and Human services FX NY and EVK are supported by intramural funds of the US Department of Health and Human services (to the National Library of Medicine). NR 56 TC 46 Z9 46 U1 0 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-422X J9 VIROL J JI Virol. J. PD APR 4 PY 2013 VL 10 AR 106 DI 10.1186/1743-422X-10-106 PG 13 WC Virology SC Virology GA 126XU UT WOS:000317656900001 PM 23557328 ER PT J AU Ramos, EM Din-Lovinescu, C Bookman, EB McNeil, LJ Baker, CC Godynskiy, G Harris, EL Lehner, T McKeon, C Moss, J Starks, VL Sherry, ST Manolio, TA Rodriguez, LL AF Ramos, Erin M. Din-Lovinescu, Corina Bookman, Ebony B. McNeil, Lisa J. Baker, Carl C. Godynskiy, Georgy Harris, Emily L. Lehner, Thomas McKeon, Catherine Moss, Joel Starks, Vaurice L. Sherry, Stephen T. Manolio, Teri. A. Rodriguez, Laura Lyman TI A Mechanism for Controlled Access to GWAS Data: Experience of the GAIN Data Access Committee SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; GENOTYPES AB The Genetic Association Information Network (GAIN) Data Access Committee was established in June 2007 to provide prompt and fair access to data from six genome-wide association studies through the database of Genotypes and Phenotypes (dbGaP). Of 945 project requests received through 2011, 749 (79%) have been approved; median receipt-to-approval time decreased from 14 days in 2007 to 8 days in 2011. Over half (54%) of the proposed research uses were for GAIN-specific phenotypes; other uses were for method development (26%) and adding controls to other studies (17%). Eight data-management incidents, defined as compromises of any of the data-use conditions, occurred among nine approved users; most were procedural violations, and none violated participant confidentiality. Over 5 years of experience with GAIN data access has demonstrated substantial use of GAIN data by investigators from academic, nonprofit, and for-profit institutions with relatively few and contained policy violations. The availability of GAIN data has allowed for advances in both the understanding of the genetic underpinnings of mental-health disorders, diabetes, and psoriasis and the development and refinement of statistical methods for identifying genetic and environmental factors related to complex common diseases. C1 [Ramos, Erin M.; Din-Lovinescu, Corina; Bookman, Ebony B.; Manolio, Teri. A.] NHGRI, Div Genom Med, NIH, Bethesda, MD 20892 USA. [McNeil, Lisa J.] Georgetown Univ, Dept Nursing, Washington, DC 20057 USA. [Baker, Carl C.] NIAMSD, Div Skin & Rheumat Dis, NIH, Bethesda, MD 20892 USA. [Godynskiy, Georgy; Sherry, Stephen T.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA. [Harris, Emily L.] NIDCR, Translat Genom Res Branch, NIH, Bethesda, MD 20892 USA. [Lehner, Thomas] NIMH, Off Genom Res Coordinat, NIH, Bethesda, MD 20892 USA. [McKeon, Catherine] NIDDKD, Div Diabet Endocrinol & Metab Dis, NIH, Bethesda, MD 20892 USA. [Moss, Joel] NHLBI, Cardiovasc & Pulm Branch, NIH, Bethesda, MD 20892 USA. [Starks, Vaurice L.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Rodriguez, Laura Lyman] NHGRI, Div Policy Commun & Educ, NIH, Bethesda, MD 20892 USA. RP Ramos, EM (reprint author), NHGRI, Div Genom Med, NIH, Bethesda, MD 20892 USA. EM ramoser@mail.nih.gov FU National Library of Medicine, National Institutes of Health FX This research was supported in part by the Intramural Research Program of the National Library of Medicine, National Institutes of Health. NR 13 TC 9 Z9 9 U1 0 U2 5 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD APR 4 PY 2013 VL 92 IS 4 BP 479 EP 488 DI 10.1016/j.ajhg.2012.08.034 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 124FR UT WOS:000317449700001 PM 23561843 ER PT J AU French, JD Ghoussaini, M Edwards, SL Meyer, KB Michailidou, K Ahmed, S Khan, S Maranian, MJ O'Reilly, M Hillman, KM Betts, JA Carro, T Bailey, PJ Dicks, E Beesley, J Tyrer, J Maia, AT Beck, A Knoblauch, NW Chen, C Kraft, P Barnes, D Gonzalez-Neira, A Alonso, MR Herrero, D Tessier, DC Vincent, D Bacot, F Luccarini, C Baynes, C Conroy, D Dennis, J Bolla, MK Wang, Q Hopper, JL Southey, MC Schmidt, MK Broeks, A Verhoef, S Cornelissen, S Muir, K Lophatananon, A Stewart-Brown, S Siriwanarangsan, P Fasching, PA Loehberg, CR Ekici, AB Beckmann, MW Peto, J Silva, IDS Johnson, N Aitken, Z Sawyer, EJ Tomlinson, I Kerin, MJ Miller, N Marme, F Schneeweiss, A Sohn, C Burwinkel, B Guenel, P Truong, T Laurent-Puig, P Menegaux, F Bojesen, SE Nordestgaard, NG Nielsen, SF Flyger, H Milne, RL Zamora, MP Perez, JIA Benitez, J Anton-Culver, H Brenner, H Muller, H Arndt, V Stegmaier, C Meind, A Lichtner, P Schmutzler, RK Engel, C Brauch, H Hamann, U Justenhoven, C Aaltonen, K Heikkila, P Aittomaki, K Blomqvist, C Matsuo, K Ito, H Iwata, H Sueta, A Bogdanova, NV Antonenkova, NN Dork, T Lindblom, A Margolin, S Mannermaa, A Kataja, V Kosma, VM Hartikainen, JM Wu, AH Tseng, CC Van Den Berg, D Stram, DO Lambrechts, D Peeters, S Smeets, A Floris, G Chang-Claude, J Rudolph, A Nickels, S Flesch-Janys, D Radice, P Peterlongo, P Bonanni, B Sardella, D Couch, FJ Wang, XS Pankratz, VS Lee, A Giles, GG Severi, G Baglietto, L Haiman, CA Henderson, BE Schumacher, F Le Marchand, L Simard, J Goldberg, MS Labreche, F Dumont, M Teo, SH Yip, CH Ng, CH Vithana, EN Kristensen, V Zheng, W Deming-Halverson, S Shrubsole, M Long, JR Winqvist, R Pylkas, K Jukkola-Vuorinen, A Grip, M Andrulis, IL Knight, JA Glendon, G Mulligan, AM Devilee, P Seynaeve, C Garcia-Closas, M Figueroa, J Chanock, SJ Lissowska, J Czene, K Klevebring, D Schoof, N Hooning, MJ Martens, JWM Collee, JM Tilanus-Linthorst, M Hall, P Li, JM Liu, JJ Humphreys, K Shu, XO Lu, W Gao, YT Cai, H Cox, A Balasubramanian, SP Blot, W Signorello, LB Cai, QY Pharoah, PDP Healey, CS Shah, M Pooley, KA Kang, D Yoo, KY Noh, DY Hartman, M Miao, H Sng, JH Sim, X Jakubowska, A Lubinski, J Jaworska-Bieniek, K Durda, K Sangrajrang, S Gaborieau, V McKay, J Toland, AE Ambrosone, CB Yannoukakos, D Godwin, AK Shen, CY Hsiung, CN Wu, PE Chen, ST Swerdlow, A Ashworth, A Orr, N Schoemaker, MJ Ponder, BAJ Nevanlinna, H Brown, MA Chenevix-Trench, G Easton, DF Dunning, AM AF French, Juliet D. Ghoussaini, Maya Edwards, Stacey L. Meyer, Kerstin B. Michailidou, Kyriaki Ahmed, Shahana Khan, Sofia Maranian, Mel J. O'Reilly, Martin Hillman, Kristine M. Betts, Joshua A. Carro, Thomas Bailey, Peter J. Dicks, Ed Beesley, Jonathan Tyrer, Jonathan Maia, Ana-Teresa Beck, Andrew Knoblauch, Nicholas W. Chen, Constance Kraft, Peter Barnes, Daniel Gonzalez-Neira, Anna Rosario Alonso, M. Herrero, Daniel Tessier, Daniel C. Vincent, Daniel Bacot, Francois Luccarini, Craig Baynes, Caroline Conroy, Don Dennis, Joe Bolla, Manjeet K. Wang, Qin Hopper, John L. Southey, Melissa C. Schmidt, Marjanka K. Broeks, Annegien Verhoef, Senno Cornelissen, Sten Muir, Kenneth Lophatananon, Artitaya Stewart-Brown, Sarah Siriwanarangsan, Pomthep Fasching, Peter A. Loehberg, Christian R. Ekici, Arif B. Beckmann, Matthias W. Peto, Julian Silva, Isabel dos Santos Johnson, Nichola Aitken, Zoe Sawyer, Elinor J. Tomlinson, Ian Kerin, Michael J. Miller, Nicola Marme, Frederik Schneeweiss, Andreas Sohn, Christof Burwinkel, Barbara Guenel, Pascal Therese Truong Laurent-Puig, Pierre Menegaux, Florence Bojesen, Stig E. Nordestgaard, Norge G. Nielsen, Sune F. Flyger, Henrik Milne, Roger L. Pilar Zamora, M. Arias Perez, Jose Ignacio Benitez, Javier Anton-Culver, Hoda Brenner, Hermann Mueller, Heiko Arndt, Volker Stegmaier, Christa Meind, Alfons Lichtner, Peter Schmutzler, Rita K. Engel, Christoph Brauch, Hiltrud Hamann, Ute Justenhoven, Christina Aaltonen, Kirsimari Heikkila, Paivi Aittomaki, Kristiina Blomqvist, Carl Matsuo, Keitaro Ito, Hidemi Iwata, Hiroji Sueta, Aiko Bogdanova, Natalia V. Antonenkova, Natalia N. Dork, Thilo Lindblom, Annika Margolin, Sara Mannermaa, Arto Kataja, Vesa Kosma, Veli-Matti Hartikainen, Jaana M. Wu, Anna H. Tseng, Chiu-chen Van Den Berg, David Stram, Daniel O. Lambrechts, Diether Peeters, Stephanie Smeets, Ann Floris, Giuseppe Chang-Claude, Jenny Rudolph, Anja Nickels, Stefan Flesch-Janys, Dieter Radice, Paolo Peterlongo, Paolo Bonanni, Bernardo Sardella, Domenico Couch, Fergus J. Wang, Xianshu Pankratz, Vernon S. Lee, Adam Giles, Graham G. Severi, Gianluca Baglietto, Laura Haiman, Christopher A. Henderson, Brian E. Schumacher, Fredrick Le Marchand, Loic Simard, Jacques Goldberg, Mark S. Labreche, France Dumont, Martine Teo, Soo Hwang Yip, Cheng Har Ng, Char-Hong Vithana, Eranga Nishanthie Kristensen, Vessela Zheng, Wei Deming-Halverson, Sandra Shrubsole, Martha Long, Jirong Winqvist, Robert Pylkas, Katri Jukkola-Vuorinen, Arja Grip, Mervi Andrulis, Irene L. Knight, Julia A. Glendon, Gord Mulligan, Anna Marie Devilee, Peter Seynaeve, Caroline Garcia-Closas, Montserrat Figueroa, Jonine Chanock, Stephen J. Lissowska, Jolanta Czene, Kamila Klevebring, Daniel Schoof, Nils Hooning, Maartje J. Martens, John W. M. Collee, J. Margriet Tilanus-Linthorst, Madeleine Hall, Per Li, Jingmei Liu, Jianjun Humphreys, Keith Shu, Xiao-Ou Lu, Wei Gao, Yu-Tang Cai, Hui Cox, Angela Balasubramanian, Sabapathy P. Blot, William Signorello, Lisa B. Cai, Qiuyin Pharoah, Paul D. P. Healey, Catherine S. Shah, Mitul Pooley, Karen A. Kang, Daehee Yoo, Keun-Young Noh, Dong-Young Hartman, Mikael Miao, Hui Sng, Jen-Hwei Sim, Xueling Jakubowska, Anna Lubinski, Jan Jaworska-Bieniek, Katarzyna Durda, Katarzyna Sangrajrang, Suleeporn Gaborieau, Valerie McKay, James Toland, Amanda E. Ambrosone, Christine B. Yannoukakos, Drakoulis Godwin, Andrew K. Shen, Chen-Yang Hsiung, Chia-Ni Wu, Pei-Ei Chen, Shou-Tung Swerdlow, Anthony Ashworth, Alan Orr, Nick Schoemaker, Minouk J. Ponder, Bruce A. J. Nevanlinna, Heli Brown, Melissa A. Chenevix-Trench, Georgia Easton, Douglas F. Dunning, Alison M. CA GENICA Network KConFab Investigators TI Functional Variants at the 11q13 Risk Locus for Breast Cancer Regulate Cyclin D1 Expression through Long-Range Enhancers SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; GROWTH-FACTOR RECEPTOR-2; ESTROGEN-RECEPTOR; PROSTATE-CANCER; SUSCEPTIBILITY LOCI; GENE-EXPRESSION; DOWN-REGULATION; MAMMARY-GLAND; MULTIPLE LOCI; CELL-CYCLE AB Analysis of 4,405 variants in 89,050 European subjects from 41 case-control studies identified three independent association signals for estrogen-receptor-positive tumors at 11q13. The strongest signal maps to a transcriptional enhancer element in which the G allele of the best candidate causative variant rs554219 increases risk of breast cancer, reduces both binding of ELK4 transcription factor and luciferase activity in reporter assays, and may be associated with low cyclin D1 protein levels in tumors. Another candidate variant, rs78540526, lies in the same enhancer element. Risk association signal 2, rs75915166, creates a GATA3 binding site within a silencer element. Chromatin conformation studies demonstrate that these enhancer and silencer elements interact with each other and with their likely target gene, CCND1. C1 [French, Juliet D.; Edwards, Stacey L.; Hillman, Kristine M.; Betts, Joshua A.; Bailey, Peter J.; Lee, Adam; Brown, Melissa A.] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia. [Ghoussaini, Maya; Ahmed, Shahana; Maranian, Mel J.; Dicks, Ed; Tyrer, Jonathan; Luccarini, Craig; Baynes, Caroline; Conroy, Don; Pharoah, Paul D. P.; Healey, Catherine S.; Shah, Mitul; Easton, Douglas F.; Dunning, Alison M.] Univ Cambridge, Ctr Canc Genet Epidemiol, Dept Oncol, Cambridge CB1 8RN, England. [Meyer, Kerstin B.; O'Reilly, Martin; Carro, Thomas; Maia, Ana-Teresa; Ponder, Bruce A. J.] Li Ka Shing Ctr, Canc Res UK Cambridge Res Inst, Cambridge CB2 ORE, England. 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[Jakubowska, Anna; Lubinski, Jan; Jaworska-Bieniek, Katarzyna; Durda, Katarzyna] Pomeranian Med Univ, Dept Genet & Pathol, PL-70115 Szczecin, Poland. [Jaworska-Bieniek, Katarzyna] Warsaw Med Univ, Postgrad Sch Mol Med, PL-02091 Warsaw, Poland. [Sangrajrang, Suleeporn] Natl Canc Inst, Bangkok 10400, Thailand. [Gaborieau, Valerie; McKay, James] Int Agcy Res Canc, F-69372 Lyon 08, France. [Toland, Amanda E.] Ohio State Univ, Ctr Comprehens Canc, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA. [Ambrosone, Christine B.] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Buffalo, NY 14263 USA. [Yannoukakos, Drakoulis] Natl Ctr Sci Res Demokritos, IRRP, Mol Diagnost Lab, Athens 15310, Greece. [Godwin, Andrew K.] Univ Kansas, Med Ctr, Dept Pathol & Lab Med, Kansas City, KS 66160 USA. [Shen, Chen-Yang] China Med Univ, Colleague Publ Hlth, Taichong 40402, Taiwan. [Shen, Chen-Yang; Hsiung, Chia-Ni] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan. [Wu, Pei-Ei] Acad Sinica, Taiwan Biobank, Inst Biomed Sci, Taipei 115, Taiwan. [Chen, Shou-Tung] Changhua Christian Hosp, Dept Surg, Changhua 500, Taiwan. RP Dunning, AM (reprint author), Univ Cambridge, Ctr Canc Genet Epidemiol, Dept Oncol, Cambridge CB1 8RN, England. EM amd24@medschl.cam.ac.uk RI Jakubowska, Anna/O-8050-2014; Yip, Cheng-Har/B-1909-2010; Gonzalez-Neira, Anna/C-5791-2015; Garcia-Closas, Montserrat /F-3871-2015; Shrubsole, Martha/K-5052-2015; Hartikainen, Jaana/E-6256-2015; laurent-puig, pierre/B-2226-2013; Ng, Char Hong/F-8462-2010; Edwards, Stacey/A-4980-2011; Brown, Melissa/F-1451-2010; Brenner, Hermann/B-4627-2017; Radice, Paolo/O-3119-2013; Knight, Julia/A-6843-2012; Maia, Ana-Teresa/F-4404-2012; Andrulis, Irene/E-7267-2013; Ekici, Arif/C-3971-2013; French, Juliet/A-4982-2011; Li, Jingmei/I-2904-2012; Toland, Amanda/E-4202-2011; Kerin, Michael/D-6748-2013; Dork, Thilo/J-8620-2012; Teo, Soo-hwang/H-2353-2014; Hartman, Mikael/B-4324-2011; Noh, Dong-Young/G-5531-2011; OI Rudolph, Anja/0000-0001-7520-2035; Klevebring, Daniel/0000-0002-9810-3838; Schoemaker, Minouk/0000-0001-8403-2234; Brown, Melissa/0000-0002-2830-9259; Czene, Kamila/0000-0002-3233-5695; Lissowska, Jolanta/0000-0003-2695-5799; Arias Perez, Jose Ignacio/0000-0003-4500-397X; Dennis, Joe/0000-0003-4591-1214; Khan, Sofia/0000-0003-4185-8882; Aitken, Zoe/0000-0002-5413-2450; Garcia-Closas, Montserrat /0000-0003-1033-2650; Shrubsole, Martha/0000-0002-5591-7575; laurent-puig, pierre/0000-0001-8475-5459; Brenner, Hermann/0000-0002-6129-1572; Devilee, Peter/0000-0002-8023-2009; Barnes, Daniel/0000-0002-3781-7570; Maia, Ana-Teresa/0000-0002-0454-9207; Li, Jingmei/0000-0001-8587-7511; Dunning, Alison Margaret/0000-0001-6651-7166; Nevanlinna, Heli/0000-0002-0916-2976; dos Santos Silva, Isabel/0000-0002-6596-8798; Cox, Angela/0000-0002-5138-1099; Yannoukakos, Drakoulis/0000-0001-7509-3510; Giles, Graham/0000-0003-4946-9099; Matsuo, Keitaro/0000-0003-1761-6314; Arndt, Volker/0000-0001-9320-8684 FU Cancer Research UK [10119, 10118, 10124]; NCI NIH HHS [P01 CA087969, P50 CA116201, R01 CA092447, R01 CA122340, R01 CA128978] NR 49 TC 82 Z9 82 U1 7 U2 60 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD APR 4 PY 2013 VL 92 IS 4 BP 489 EP 503 DI 10.1016/j.ajhg.2013.01.002 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA 124FR UT WOS:000317449700002 PM 23540573 ER PT J AU Keupp, K Beleggia, F Kayserili, H Barnes, AM Steiner, M Semler, O Fischer, B Yigit, G Janda, CY Becker, J Breer, S Altunoglu, U Grunhagen, J Krawitz, P Hecht, J Schinke, T Makareeva, E Lausch, E Cankaya, T Caparros-Martin, JA Lapunzina, P Temtamy, S Aglan, M Zabel, B Eysel, P Koerber, F Leikin, S Garcia, KC Netzer, C Schonau, E Ruiz-Perez, VL Mundlos, S Amling, M Kornak, U Marini, J Wollnik, B AF Keupp, Katharina Beleggia, Filippo Kayserili, Hulya Barnes, Aileen M. Steiner, Magdalena Semler, Oliver Fischer, Bjoern Yigit, Goekhan Janda, Claudia Y. Becker, Jutta Breer, Stefan Altunoglu, Umut Gruenhagen, Johannes Krawitz, Peter Hecht, Jochen Schinke, Thorsten Makareeva, Elena Lausch, Ekkehart Cankaya, Tufan Caparros-Martin, Jose A. Lapunzina, Pablo Temtamy, Samia Aglan, Mona Zabel, Bernhard Eysel, Peer Koerber, Friederike Leikin, Sergey Garcia, K. Christopher Netzer, Christian Schoenau, Eckhard Ruiz-Perez, Victor L. Mundlos, Stefan Amling, Michael Kornak, Uwe Marini, Joan Wollnik, Bernd TI Mutations in WNT1 Cause Different Forms of Bone Fragility SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID RECESSIVE OSTEOGENESIS IMPERFECTA; OSTEOPOROSIS-PSEUDOGLIOMA SYNDROME; MINERAL DENSITY; IN-VIVO; IDENTIFICATION; PROTOONCOGENE; METAANALYSIS; DISORDERS; FRACTURE; REVEALS AB We report that hypofunctional alleles of WNT1 cause autosomal-recessive osteogenesis imperfecta, a congenital disorder characterized by reduced bone mass and recurrent fractures. In consanguineous families, we identified five homozygous mutations in WNT1: one frameshift mutation, two missense mutations, one splice-site mutation, and one nonsense mutation. In addition, in a family affected by dominantly inherited early-onset osteoporosis, a heterozygous WNT1 missense mutation was identified in affected individuals. Initial functional analysis revealed that altered WNT1 proteins fail to activate canonical LRP5-mediated WNT-regulated beta-catenin signaling. Furthermore, osteoblasts cultured in vitro showed enhanced Wnt1 expression with advancing differentiation, indicating a role of WNT1 in osteoblast function and bone development. Our finding that homozygous and heterozygous variants in WNT1 predispose to low-bone-mass phenotypes might advance the development of more effective therapeutic strategies for congenital forms of bone fragility, as well as for common forms of age-related osteoporosis. C1 [Keupp, Katharina; Beleggia, Filippo; Yigit, Goekhan; Becker, Jutta; Netzer, Christian; Wollnik, Bernd] Univ Cologne, Univ Hosp Cologne, Inst Human Genet, D-50931 Cologne, Germany. [Keupp, Katharina; Beleggia, Filippo; Yigit, Goekhan; Wollnik, Bernd] Univ Cologne, Ctr Mol Med Cologne, D-50931 Cologne, Germany. [Keupp, Katharina; Beleggia, Filippo; Yigit, Goekhan; Wollnik, Bernd] Univ Cologne, Cologne Excellence Cluster Cellular Stress Respon, D-50931 Cologne, Germany. [Kayserili, Hulya; Altunoglu, Umut] Istanbul Univ, Istanbul Fac Med, Dept Med Genet, TR-34093 Istanbul, Turkey. [Barnes, Aileen M.; Marini, Joan] NICHHD, Bone & Extracellular Matrix Branch, NIH, Bethesda, MD 20892 USA. [Steiner, Magdalena; Fischer, Bjoern; Gruenhagen, Johannes; Krawitz, Peter; Mundlos, Stefan; Kornak, Uwe] Charite, Inst Med Genet & Human Genet, D-13353 Berlin, Germany. [Semler, Oliver; Schoenau, Eckhard] Univ Cologne, Childrens Hosp, D-50931 Cologne, Germany. [Janda, Claudia Y.; Garcia, K. Christopher] Stanford Univ, Sch Med, Howard Hughes Med Inst, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA. [Janda, Claudia Y.; Garcia, K. Christopher] Stanford Univ, Sch Med, Howard Hughes Med Inst, Dept Biol Struct, Stanford, CA 94305 USA. [Breer, Stefan; Schinke, Thorsten; Amling, Michael] Univ Med Ctr Hamburg Eppendorf, Dept Osteol & Biomech, D-20246 Hamburg, Germany. [Hecht, Jochen] Berlin Brandenburg Ctr Regenerat Therapies, D-13353 Berlin, Germany. [Makareeva, Elena; Leikin, Sergey] NICHHD, Sect Phys Biochem, NIH, Bethesda, MD 20892 USA. [Lausch, Ekkehart; Zabel, Bernhard] Univ Freiburg, Childrens Hosp, Div Genet, D-79106 Freiburg, Germany. [Cankaya, Tufan] Dokuz Eylul Univ, Fac Med, Dept Med Genet, TR-35210 Izmir, Turkey. [Caparros-Martin, Jose A.; Ruiz-Perez, Victor L.] CSIC, Inst Invest Biomed, E-28029 Madrid, Spain. [Caparros-Martin, Jose A.; Ruiz-Perez, Victor L.] Univ Autonoma Madrid, Madrid 28029, Spain. [Caparros-Martin, Jose A.; Lapunzina, Pablo; Ruiz-Perez, Victor L.] Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Raras, Madrid 28029, Spain. [Lapunzina, Pablo] Univ Autonoma Madrid, Inst Genet Med & Mol, Inst Invest Hosp Univ La Paz, Madrid 28046, Spain. [Temtamy, Samia; Aglan, Mona] Natl Res Ctr, Human Genet & Genome Res Div, Cairo 12311, Egypt. [Eysel, Peer] Univ Cologne, Dept Orthopaed & Traumat Surg, D-50931 Cologne, Germany. [Koerber, Friederike] Univ Cologne, Dept Radiol, D-50931 Cologne, Germany. [Mundlos, Stefan; Kornak, Uwe] Max Planck Inst Mol Genet, D-14195 Berlin, Germany. RP Kornak, U (reprint author), Charite, Inst Med Genet & Human Genet, D-13353 Berlin, Germany. EM uwe.kornak@charite.de; bwollnik@uni-koeln.de RI Fischer-Zirnsak, Bjorn/D-7487-2013; Krawitz, Peter/F-3142-2012; Makareeva, Elena/F-5183-2011; Ruiz-Perez, Victor /K-6066-2014; Leikin, Sergey/A-5518-2008; OI Krawitz, Peter/0000-0002-3194-8625; Ruiz-Perez, Victor /0000-0003-0635-3619; Leikin, Sergey/0000-0001-7095-0739; Semler, Oliver/0000-0003-0029-7556; aglan, mona/0000-0002-0908-0639; temtamy, samia/0000-0002-6452-823X; Caparros-Martin, Jose A./0000-0003-1214-4952 FU German Federal Ministry of Education and Research [01GM1211A, 01GM1109C, 01EC1006A]; TUBITAK [112S398]; National Institute of Child Health and Human Development (National Institutes of Health) FX We are grateful to all family members who participated in this study, to Esther Milz for excellent technical assistance, and to Karin Boss for critically reading the manuscript. We want to thank Claus-Eric Ott for help with cDNAs and Boi-Dinh Chung-Ueck for kindly providing expression vectors. This work was supported by German Federal Ministry of Education and Research grants 01GM1211A (E-RARE network CRANIRARE-2), 01GM1109C (national rare disease network Forschungsverbund Ausgewahlter Craniofacialer Entwicklungsstorungen) to B.W., and 01EC1006A (Molecular Pathogenesis of Osteoporosis) to U.K. and S.M., by TUBITAK grant 112S398 (E-RARE network CRANIRARE-2) to H.K., and by National Institute of Child Health and Human Development (National Institutes of Health) Intramural Research Program funding to S.L. and J.C.M. M.S. holds a Doc female forte scholarship from Austria and is a member of the Berlin-Brandenburg School for Regenerative Therapies. NR 36 TC 66 Z9 72 U1 2 U2 21 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD APR 4 PY 2013 VL 92 IS 4 BP 565 EP 574 DI 10.1016/j.ajhg.2013.02.010 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 124FR UT WOS:000317449700008 PM 23499309 ER PT J AU Shaheen, R Aglan, M Keppler-Noreuil, K Faqeih, E Ansari, S Horton, K Ashour, A Zaki, MS Al-Zahrani, F Cueto-Gonzalez, AM Abdel-Salam, G Temtamy, S Alkuraya, FS AF Shaheen, Ranad Aglan, Mona Keppler-Noreuil, Kim Faqeih, Eissa Ansari, Shinu Horton, Kim Ashour, Adel Zaki, Maha S. Al-Zahrani, Fatema Cueto-Gonzalez, Anna M. Abdel-Salam, Ghada Temtamy, Samia Alkuraya, Fowzan S. TI Mutations in EOGT Confirm the Genetic Heterogeneity of Autosomal-Recessive Adams-Oliver Syndrome SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID LINKED-N-ACETYLGLUCOSAMINE; O-GLCNAC; NOTCH RECEPTORS; PHOSPHORYLATION; GLCNACYLATION; PROTEINS; APLASIA; DOMAIN; TAU AB Adams-Oliver syndrome (AOS) is a rare, autosomal-dominant or -recessive disorder characterized primarily by aplasia cutis congenita and terminal transverse limb defects. Recently, we demonstrated that homozygous mutations in DOCK6 cause an autosomal-recessive form of AOS. In this study, we sought to determine the contribution of DOCK6 mutations to the etiology of AOS in several consanguineous families. In two of the five families studied, we identified two homozygous truncating mutations (a splice-site mutation and a frameshift duplication). DOCK6 sequencing revealed no mutation in the remaining three families, consistent with their autozygosity mapping and linkage-analysis results, which revealed a single candidate locus in 3p14.1 on three different haplotype backgrounds in the three families. Indeed, exome sequencing in one family revealed one missense mutation in EOGT (C3orf64), and subsequent targeted sequencing of this gene revealed a homozygous missense mutation and a homozygous frameshift deletion mutation in the other two families. EOGT encodes EGF-domain-specific O-linked N-acetylglucosamine (O-GlcNAc) transferase, which is involved in the O-GlcNAcylation (attachment of O-GlcNAc to serine and threonine residues) of a subset of extracellular EGF-domain-containing proteins. It has a documented role in epithelial-cell-matrix interactions in Drosophila, in which deficiency of its ortholog causes wing blistering. Our findings highlight a developmental role of O-GlcNAcylation in humans and expand the genetic heterogeneity of autosomal-recessive AOS. C1 [Shaheen, Ranad; Ansari, Shinu; Al-Zahrani, Fatema; Alkuraya, Fowzan S.] King Faisal Specialist Hosp & Res Ctr, Dept Genet, Riyadh 11211, Saudi Arabia. [Aglan, Mona; Ashour, Adel; Zaki, Maha S.; Abdel-Salam, Ghada; Temtamy, Samia] Natl Res Ctr, Dept Clin Genet, Cairo 12311, Egypt. [Keppler-Noreuil, Kim] NHGRI, Genet Dis Res Branch, Bethesda, MD 20892 USA. [Faqeih, Eissa] King Fahad Med City, Med Genet Sect, Dept Pediat, Riyadh 11525, Saudi Arabia. [Horton, Kim] Univ Iowa, Reg Genet Consultat Serv, Iowa City, IA 52242 USA. [Cueto-Gonzalez, Anna M.] Hosp Univ Vall dHebron, Clin Genet Serv, Barcelona 08035, Spain. [Alkuraya, Fowzan S.] Alfaisal Univ, Coll Med, Dept Anat & Cell Biol, Riyadh 11533, Saudi Arabia. RP Alkuraya, FS (reprint author), King Faisal Specialist Hosp & Res Ctr, Dept Genet, POB 3354, Riyadh 11211, Saudi Arabia. EM falkuraya@kfshrc.edu.sa OI aglan, mona/0000-0002-0908-0639; temtamy, samia/0000-0002-6452-823X; Abdel-Salam, Ghada/0000-0003-2893-8802; Zaki, Maha/0000-0001-7840-0002 FU King Abdulaziz City for Science and Technology [09-MED941-20]; Dubai Harvard Foundation for Medical Research Collaborative Research Center grant FX We thank the affected individuals and their families for their enthusiastic participation. We are grateful to Mais Hashem and Niema Ibrahim for their help as clinical research coordinators. We also thank the Genotyping and Sequencing Core Facilities at King Faisal Specialist Hospital and Research Center for their technical help. This work was supported by King Abdulaziz City for Science and Technology grant 09-MED941-20 (to F.S.A.) and a Dubai Harvard Foundation for Medical Research Collaborative Research Center grant (to F.S.A.). NR 27 TC 31 Z9 33 U1 0 U2 9 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD APR 4 PY 2013 VL 92 IS 4 BP 598 EP 604 DI 10.1016/j.ajhg.2013.02.012 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA 124FR UT WOS:000317449700012 PM 23522784 ER PT J AU Jenkinson, EM Rehman, AU Walsh, T Clayton-Smith, J Lee, K Morell, RJ Drummond, MC Khan, SN Naeem, MA Rauf, B Billington, N Schultz, JM Urquhart, JE Lee, MK Berry, A Hanley, NA Mehta, S Cilliers, D Clayton, PE Kingston, H Smith, MJ Warner, TT Black, GC Trump, D Davis, JRE Ahmad, W Leal, SM Riazuddin, S King, MC Friedman, TB Newman, WG AF Jenkinson, Emma M. Rehman, Atteeq U. Walsh, Tom Clayton-Smith, Jill Lee, Kwanghyuk Morell, Robert J. Drummond, Meghan C. Khan, Shaheen N. Naeem, Muhammad Asif Rauf, Bushra Billington, Neil Schultz, Julie M. Urquhart, Jill E. Lee, Ming K. Berry, Andrew Hanley, Neil A. Mehta, Sarju Cilliers, Deirdre Clayton, Peter E. Kingston, Helen Smith, Miriam J. Warner, Thomas T. Black, Graeme C. Trump, Dorothy Davis, Julian R. E. Ahmad, Wasim Leal, Suzanne M. Riazuddin, Sheikh King, Mary-Claire Friedman, Thomas B. Newman, William G. CA Univ Washington TI Perrault Syndrome Is Caused by Recessive Mutations in CLPP, Encoding a Mitochondrial ATP-Dependent Chambered Protease SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID HEREDITARY SPASTIC PARAPLEGIA; CAUSE OVARIAN DYSGENESIS; ESCHERICHIA-COLI CLPP; HEARING-LOSS; EXPRESSION; SUBUNIT; COMPLEX; GENE; RNA; ACTIVATION AB Perrault syndrome is a genetically and clinically heterogeneous autosomal-recessive condition characterized by sensorineural hearing loss and ovarian failure. By a combination of linkage analysis, homozygosity mapping, and exome sequencing in three families, we identified mutations in CLPP as the likely cause of this phenotype. In each family, affected individuals were homozygous for a different pathogenic CLPP allele: c.433A>C (p.Thr145Pro), c.440G>C (p.Cys147Ser), or an experimentally demonstrated splice-donor-site mutation, c.270+4A>G. CLPP, a component of a mitochondrial ATP-dependent proteolytic complex, is a highly conserved endopeptidase encoded by CLPP and forms an element of the evolutionarily ancient mitochondrial unfolded-protein response (UPRmt) stress signaling pathway. Crystal-structure modeling suggests that both substitutions would alter the structure of the CLPP barrel chamber that captures unfolded proteins and exposes them to proteolysis. Together with the previous identification of mutations in HARS2, encoding mitochondrial histidyl-tRNA synthetase, mutations in CLPP expose dysfunction of mitochondrial protein homeostasis as a cause of Perrault syndrome. C1 [Jenkinson, Emma M.; Clayton-Smith, Jill; Urquhart, Jill E.; Kingston, Helen; Smith, Miriam J.; Black, Graeme C.; Trump, Dorothy; Newman, William G.] Univ Manchester, Fac Med & Human Sci, Inst Human Dev, Ctr Genet Med, Manchester M13 9WL, Lancs, England. [Jenkinson, Emma M.; Clayton-Smith, Jill; Urquhart, Jill E.; Berry, Andrew; Hanley, Neil A.; Kingston, Helen; Smith, Miriam J.; Black, Graeme C.; Trump, Dorothy; Davis, Julian R. E.; Newman, William G.] Cent Manchester Univ Hosp NHS Fdn Trust, Manchester M13 9WL, Lancs, England. [Rehman, Atteeq U.; Morell, Robert J.; Drummond, Meghan C.; Schultz, Julie M.; Friedman, Thomas B.] NIDCD, Mol Genet Lab, NIH, Rockville, MD 20850 USA. [Walsh, Tom; Lee, Ming K.; King, Mary-Claire] Univ Washington, Dept Med, Div Med Genet, Seattle, WA 98195 USA. [Lee, Kwanghyuk; Leal, Suzanne M.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Khan, Shaheen N.; Naeem, Muhammad Asif; Rauf, Bushra; Riazuddin, Sheikh] Univ Punjab, Ctr Excellence Mol Biol, Lahore 54590, Pakistan. [Billington, Neil] NHLBI, Lab Mol Physiol, NIH, Bethesda, MD 20824 USA. [Berry, Andrew; Hanley, Neil A.; Davis, Julian R. E.] Univ Manchester, Fac Med & Human Sci, Inst Human Dev, Ctr Endocrinol & Diabet, Manchester M13 9WL, Lancs, England. [Mehta, Sarju] Addenbrookes Hosp, East Anglian Med Genet Serv, Cambridge CB2 0QQ, England. [Cilliers, Deirdre] Oxford Univ NHS Trust, Oxford Reg Genet Ctr, Oxford OX3 7LJ, England. [Clayton, Peter E.] Univ Manchester, Inst Human Dev, Fac Med & Human Sci, Manchester M13 9WL, Lancs, England. [Clayton, Peter E.] Cent Manchester Fdn NHS Trust, Manchester M13 9WL, Lancs, England. [Warner, Thomas T.] UCL, Inst Neurol, London WC1N 3BG, England. [Ahmad, Wasim] Quaid I Azam Univ, Fac Biol Sci, Dept Biochem, Islamabad 45320, Pakistan. [Riazuddin, Sheikh] Univ Hlth Sci, Allama Iqbal Med Coll, Lahore 54550, Pakistan. RP Friedman, TB (reprint author), NIDCD, Mol Genet Lab, NIH, Rockville, MD 20850 USA. EM friedman@nidcd.nih.gov; william.newman@manchester.ac.uk RI sebastianovitsch, stepan/G-8507-2013; Warner, Thomas/A-1454-2013; Davis, Julian/G-8943-2014; Urquhart, Jill/G-2282-2015; Ahmad, Wasim/E-9713-2015; OI Walsh, Tom/0000-0002-8875-0310; Morell, Robert/0000-0003-1537-7356; Hanley, Neil/0000-0003-3234-4038; Warner, Thomas/0000-0001-6195-6995; Davis, Julian/0000-0003-0838-0557; Urquhart, Jill/0000-0002-5788-5511; Lee, Kwanghyuk/0000-0001-7781-9696; Billington, Neil/0000-0003-2306-0228; Black, Graeme/0000-0001-8727-6592; Clayton, Peter/0000-0003-1225-4537; Newman, William/0000-0002-6382-4678 FU Infertility Research Trust; Manchester University Biomedical Research Centre; Wellcome Trust; National Institutes of Health (NIH) [R01 DC005641, R01 DC011651, R01 DC003594, N01 HG065403, U54 HG006493, N01-HG-65403]; Higher Education Commission; Ministry of Science and Technology of Pakistan; International Center for Genetic Engineering and Biotechnology, Trieste, Italy [CRP/PAK08-01, 08/009]; National Heart, Lung, and Blood Institute (NIH) [HL004232]; Genome Canada through the Ontario Genomics Institute [2007-OGI-TD-05]; Ontario Ministry of Research and Innovation; [DC000039-15] FX We thank the families for their participation in the study. We thank Inna Belyantseva, Dennis Drayna, and Andrew J. Griffith for their critique of this manuscript. This study was supported by the Infertility Research Trust; by the Manchester University Biomedical Research Centre; by the Wellcome Trust (N.A.H. is a Senior Fellow in Clinical Science); by National Institutes of Health (NIH) grants and contracts R01 DC005641, R01 DC011651, R01 DC003594, N01 HG065403, and U54 HG006493; by the Higher Education Commission and the Ministry of Science and Technology of Pakistan; and by the International Center for Genetic Engineering and Biotechnology, Trieste, Italy (CRP/PAK08-01 contract 08/009 to Sh.R). Genotyping of family DEM4395 was performed at the Center for Inherited Disease Research, which is funded through the NIH to The Johns Hopkins University (contract number N01-HG-65403). N.B. was supported by National Heart, Lung, and Blood Institute (NIH) intramural funds (HL004232) to James Sellers. Work at the National Institute on Deafness and Other Communication Disorders (NIH) was supported by intramural funds (DC000039-15) to T.B.F. The authors also acknowledge GeneMANIA, the development of which was funded by Genome Canada through the Ontario Genomics Institute (2007-OGI-TD-05) and which is now funded by the Ontario Ministry of Research and Innovation. NR 44 TC 44 Z9 49 U1 0 U2 9 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD APR 4 PY 2013 VL 92 IS 4 BP 605 EP 613 DI 10.1016/j.ajhg.2013.02.013 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA 124FR UT WOS:000317449700013 PM 23541340 ER PT J AU Jasinska, AJ AF Jasinska, Agnes J. TI Automatic inhibition and habitual control: alternative views in neuroscience research on response inhibition and inhibitory control SO FRONTIERS IN BEHAVIORAL NEUROSCIENCE LA English DT Editorial Material ID INFERIOR FRONTAL GYRUS; GO/NO-GO TASKS; PREFRONTAL CORTEX; STOP-SIGNAL; APPETITIVE MOTIVATION; COGNITIVE CONTROL; ERROR-DETECTION; FACIAL SIGNALS; ACTIVATION; BEHAVIOR C1 NIDA, Intramural Res Program, Neuroimaging Res Branch, Baltimore, MD USA. RP Jasinska, AJ (reprint author), NIDA, Intramural Res Program, Neuroimaging Res Branch, Baltimore, MD USA. EM jasinskaaj@mail.nih.gov NR 49 TC 12 Z9 12 U1 1 U2 27 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1662-5153 J9 FRONT BEHAV NEUROSCI JI Front. Behav. Neurosci. PD APR 4 PY 2013 VL 7 AR 25 DI 10.3389/fnbeh.2013.00025 PG 4 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA 118NZ UT WOS:000317033500001 PM 23576964 ER PT J AU Agalliu, I Wang, ZM Wang, T Dunn, A Parikh, H Myers, T Burk, RD Amundadottir, L AF Agalliu, Ilir Wang, Zhaoming Wang, Tao Dunn, Anne Parikh, Hemang Myers, Timothy Burk, Robert D. Amundadottir, Laufey TI Characterization of SNPs Associated with Prostate Cancer in Men of Ashkenazic Descent from the Set of GWAS Identified SNPs: Impact of Cancer Family History and Cumulative SNP Risk Prediction SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; SUSCEPTIBILITY LOCUS; JEWISH POPULATION; GENETIC-VARIANTS; MULTIPLE; DISEASE; 8Q24; METAANALYSIS; CONSORTIUM; RELATIVES AB Background: Genome-wide association studies (GWAS) have identified multiple SNPs associated with prostate cancer (PrCa). Population isolates may have different sets of risk alleles for PrCa constituting unique population and individual risk profiles. Methods: To test this hypothesis, associations between 31 GWAS SNPs of PrCa were examined among 979 PrCa cases and 1,251 controls of Ashkenazic descent using logistic regression. We also investigated risks by age at diagnosis, pathological features of PrCa, and family history of cancer. Moreover, we examined associations between cumulative number of risk alleles and PrCa and assessed the utility of risk alleles in PrCa risk prediction by comparing the area under the curve (AUC) for different logistic models. Results: Of the 31 genotyped SNPs, 8 were associated with PrCa at p <= 0.002 (corrected p-value threshold) with odds ratios (ORs) ranging from 1.22 to 1.42 per risk allele. Four SNPs were associated with aggressive PrCa, while three other SNPs showed potential interactions for PrCa by family history of PrCa (rs8102476; 19q13), lung cancer (rs17021918; 4q22), and breast cancer (rs10896449; 11q13). Men in the highest vs. lowest quartile of cumulative number of risk alleles had ORs of 3.70 (95% CI 2.76-4.97); 3.76 (95% CI 2.57-5.50), and 5.20 (95% CI 2.94-9.19) for overall PrCa, aggressive cancer and younger age at diagnosis, respectively. The addition of cumulative risk alleles to the model containing age at diagnosis and family history of PrCa yielded a slightly higher AUC (0.69 vs. 0.64). Conclusion: These data define a set of risk alleles associated with PrCa in men of Ashkenazic descent and indicate possible genetic differences for PrCa between populations of European and Ashkenazic ancestry. Use of genetic markers might provide an opportunity to identify men at highest risk for younger age of onset PrCa; however, their clinical utility in identifying men at highest risk for aggressive cancer remains limited. C1 [Agalliu, Ilir; Wang, Tao; Burk, Robert D.] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10467 USA. [Wang, Zhaoming; Myers, Timothy] NCI, Canc Genom Res Lab CGR, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Dunn, Anne; Burk, Robert D.] Albert Einstein Coll Med, Dept Pediat Genet, Bronx, NY 10467 USA. [Parikh, Hemang; Myers, Timothy; Amundadottir, Laufey] NCI, Lab Translat Genom, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Burk, Robert D.] Albert Einstein Coll Med, Bronx, NY 10467 USA. RP Agalliu, I (reprint author), Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10467 USA. EM ilir.agalliu@einstein.yu.edu RI Amundadottir, Laufey/L-7656-2016 OI Amundadottir, Laufey/0000-0003-1859-8971 FU U.S. Army, Department of Defense grant [PC001076]; Einstein Cancer Research Center from the National Cancer Institute [P30CA013330]; Translational-Medicine grant from the Albert Einstein College of Medicine; Intramural Program of the National Cancer Institute/National Institutes of Health; Mentored Research Scholar Grant from the American Cancer Society [MRSG-11-112-01-CNE] FX This project was supported in part by the U.S. Army, Department of Defense grant (PC001076), the Einstein Cancer Research Center (P30CA013330) from the National Cancer Institute, by a Translational-Medicine grant from the Albert Einstein College of Medicine, and from the Intramural Program of the National Cancer Institute/National Institutes of Health. Dr. Ilir Agalliu was supported in part by a Mentored Research Scholar Grant (MRSG-11-112-01-CNE) from the American Cancer Society. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 9 Z9 9 U1 0 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 3 PY 2013 VL 8 IS 4 AR e60083 DI 10.1371/journal.pone.0060083 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 143BA UT WOS:000318840100047 PM 23573233 ER PT J AU Cohen, SS Matthews, CE Signorello, LB Schlundt, DG Blot, WJ Buchowski, MS AF Cohen, Sarah S. Matthews, Charles E. Signorello, Lisa B. Schlundt, David G. Blot, William J. Buchowski, Maciej S. TI Sedentary and Physically Active Behavior Patterns Among Low-Income African-American and White Adults Living in the Southeastern United States SO PLOS ONE LA English DT Article ID SOUTHERN COMMUNITY COHORT; TYPE-2 DIABETES-MELLITUS; LEISURE-TIME; US ADULTS; HEALTH DISPARITIES; ENERGY-EXPENDITURE; SOCIAL-CLASS; ALL-CAUSE; MORTALITY; WOMEN AB Increased sedentary behavior and lack of physical activity are associated with increased risk for many chronic diseases. Differences in leisure-time physical activity between African American and white adults have been suggested to partially explain racial disparities in chronic disease outcomes, but expanding the definition of physical activity to include household and occupational activities may reduce or even eliminate racial differences in total physical activity. The objective of this study was to describe patterns of active and sedentary behaviors in black and white adults and to examine these behaviors across demographic measures. Sedentary and physically active behaviors were obtained from a validated physical activity questionnaire in 23,021 black men, 9,899 white men, 32,214 black women, and 15,425 white women (age 40-79) at enrollment into the Southern Community Cohort Study. Descriptive statistics for sedentary time; light, moderate, and vigorous household/occupational activity; sports/exercise; total activity; and meeting current physical activity recommendations via sports/exercise were examined for each race-sex group. Adjusted means were calculated using multiple linear regression models across demographic measures. Study participants spent approximately 60% of waking time in sedentary behaviors. Blacks reported more television viewing time than whites (45 minutes for females, 15 minutes for males), but when sitting time was expressed as a proportion of overall awake time, minimal racial differences were found. Patterns of light, moderate, and vigorous household/occupational activity were similar in all race/sex groups. 2008 Physical Activity Guidelines for Americans were followed by 16% of women and 25% of men independent of race. Overall, black and white men and women in this study spent the majority of their daily time in sedentary behaviors and less than one-fourth followed current guidelines for physical activity. These results indicate that public health campaigns should focus on both reducing sedentary behavior and increasing physical activity in all adult US populations. C1 [Cohen, Sarah S.; Blot, William J.] Int Epidemiol Inst, Rockville, MD USA. [Matthews, Charles E.] NCI, Div Canc Epidemiol & Genet, Nutr Epidemiol Branch, Rockville, MD USA. [Signorello, Lisa B.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Schlundt, David G.] Vanderbilt Univ, Dept Psychol, Nashville, TN 37240 USA. [Blot, William J.; Buchowski, Maciej S.] Vanderbilt Univ, Sch Med, Div Epidemiol, Dept Med,Vanderbilt Epidemiol Ctr, Nashville, TN 37240 USA. [Blot, William J.; Buchowski, Maciej S.] Vanderbilt Ingram Canc Ctr, Nashville, TN USA. [Buchowski, Maciej S.] Vanderbilt Univ, Sch Med, Dept Med, Div Gastroenterol Hepatol & Nutr, Nashville, TN 37212 USA. RP Cohen, SS (reprint author), Int Epidemiol Inst, Rockville, MD USA. EM sarah@iei.us RI matthews, Charles/E-8073-2015; OI matthews, Charles/0000-0001-8037-3103; Buchowski, Maciej/0000-0002-0566-1743 FU National Cancer Institute [R01CA092447] FX The Southern Community Cohort Study is funded by the National Cancer Institute (R01CA092447). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 16 Z9 16 U1 2 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 3 PY 2013 VL 8 IS 4 AR e59975 DI 10.1371/journal.pone.0059975 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 143BA UT WOS:000318840100038 PM 23573224 ER PT J AU Mcgowan, I Hoesley, C Cranston, RD Andrew, P Janocko, L Dai, JY Carballo-Dieguez, A Ayudhya, RKN Piper, J Hladik, F Mayer, K AF Mcgowan, Ian Hoesley, Craig Cranston, Ross D. Andrew, Philip Janocko, Laura Dai, James Y. Carballo-Dieguez, Alex Ayudhya, Ratiya Kunjara Na Piper, Jeanna Hladik, Florian Mayer, Ken TI A Phase 1 Randomized, Double Blind, Placebo Controlled Rectal Safety and Acceptability Study of Tenofovir 1% Gel (MTN-007) SO PLOS ONE LA English DT Article ID ANAL INTERCOURSE; ULCERATIVE-COLITIS; HIV-INFECTION; MICROBICIDE; MEN; INFLAMMATION; TRIALS; MODEL; RISK; SEX AB Objective: Rectal microbicides are needed to reduce the risk of HIV acquisition associated with unprotected receptive anal intercourse. The MTN-007 study was designed to assess the safety (general and mucosal), adherence, and acceptability of a new reduced glycerin formulation of tenofovir 1% gel. Methods: Participants were randomized 1:1:1:1 to receive the reduced glycerin formulation of tenofovir 1% gel, a hydroxyethyl cellulose placebo gel, a 2% nonoxynol-9 gel, or no treatment. Each gel was administered as a single dose followed by 7 daily doses. Mucosal safety evaluation included histology, fecal calprotectin, epithelial sloughing, cytokine expression (mRNA and protein), microarrays, flow cytometry of mucosal T cell phenotype, and rectal microflora. Acceptability and adherence were determined by computer-administered questionnaires and interactive telephone response, respectively. Results: Sixty-five participants (45 men and 20 women) were recruited into the study. There were no significant differences between the numbers of >= Grade 2 adverse events across the arms of the study. Likelihood of future product use (acceptability) was 87% (reduced glycerin formulation of tenofovir 1% gel), 93% (hydroxyethyl cellulose placebo gel), and 63% (nonoxynol-9 gel). Fecal calprotectin, rectal microflora, and epithelial sloughing did not differ by treatment arms during the study. Suggestive evidence of differences was seen in histology, mucosal gene expression, protein expression, and T cell phenotype. These changes were mostly confined to comparisons between the nonoxynol-9 gel and other study arms. Conclusions: The reduced glycerin formulation of tenofovir 1% gel was safe and well tolerated rectally and should be advanced to Phase 2 development. C1 [Mcgowan, Ian; Cranston, Ross D.] Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA. [Mcgowan, Ian; Janocko, Laura; Ayudhya, Ratiya Kunjara Na] Magee Womens Res Inst, Microbicide Trials Network, Pittsburgh, PA USA. [Hoesley, Craig] Univ Alabama Birmingham, Birmingham, AL USA. [Andrew, Philip] FHI 360, Durham, NC USA. [Dai, James Y.; Hladik, Florian] Univ Washington, Seattle, WA 98195 USA. [Dai, James Y.; Hladik, Florian] Fred Hutchinson Canc Res Ctr, Stat Ctr HIV AIDS Res & Prevent, Seattle, WA 98104 USA. [Carballo-Dieguez, Alex] Columbia Univ, New York, NY USA. [Piper, Jeanna] NIAID, Div Aids, NIH, Bethesda, MD 20892 USA. [Mayer, Ken] Fenway Hlth, Boston, MA USA. RP Mcgowan, I (reprint author), Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA. EM imcgowan@pitt.edu OI Hladik, Florian/0000-0002-0375-2764; Cranston, Ross/0000-0002-2687-6217 FU Microbicide Trials Network (MTN); National Institute of Allergy and Infectious Diseases; Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Mental Health; U.S. National Institutes of Health [5UM1AI068633]; NIH [UM1AI068615] FX This study was funded by the Microbicide Trials Network (MTN) grant, an HIV/AIDS clinical trials network funded by the National Institute of Allergy and Infectious Diseases with co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the National Institute of Mental Health, all components of the U.S. National Institutes of Health (5UM1AI068633). This cooperative agreement involved the active collaboration of the sponsor, NIH, with the grant recipients within the MTN. SCHARP received funding under a separate NIH cooperative grant (UM1AI068615). NIH and the Statistical Center for HIV/AIDS Research & Prevention (SCHARP) employees are authors on this paper. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 40 Z9 41 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 3 PY 2013 VL 8 IS 4 AR e60147 DI 10.1371/journal.pone.0060147 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 143BA UT WOS:000318840100050 PM 23573238 ER PT J AU Weissgerber, TL Gandley, RE McGee, PL Spong, CY Myatt, L Leveno, KJ Thorp, JM Mercer, BM Peaceman, AM Ramin, SM Carpenter, MW Samuels, P Sciscione, A Harper, M Tolosa, JE Saade, G Sorokin, Y AF Weissgerber, Tracey L. Gandley, Robin E. McGee, Paula L. Spong, Catherine Y. Myatt, Leslie Leveno, Kenneth J. Thorp, John M., Jr. Mercer, Brian M. Peaceman, Alan M. Ramin, Susan M. Carpenter, Marshall W. Samuels, Philip Sciscione, Anthony Harper, Margaret Tolosa, Jorge E. Saade, George Sorokin, Yoram CA Eunice Kennedy Shriver Natl Inst TI Haptoglobin Phenotype, Preeclampsia Risk and the Efficacy of Vitamin C and E Supplementation to Prevent Preeclampsia in a Racially Diverse Population SO PLOS ONE LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; WORLD-HEALTH-ORGANIZATION; PLACEBO-CONTROLLED TRIAL; MIDDLE-AGED INDIVIDUALS; DIABETES-MELLITUS; CARDIOVASCULAR-DISEASE; PREGNANT-WOMEN; POSTMENOPAUSAL WOMEN; ASCORBIC-ACID; 2-2 GENOTYPE AB Haptoglobin's (Hp) antioxidant and pro-angiogenic properties differ between the 1-1, 2-1, and 2-2 phenotypes. Hp phenotype affects cardiovascular disease risk and treatment response to antioxidant vitamins in some non-pregnant populations. We previously demonstrated that preeclampsia risk was doubled in white Hp 2-1 women, compared to Hp 1-1 women. Our objectives were to determine whether we could reproduce this finding in a larger cohort, and to determine whether Hp phenotype influences lack of efficacy of antioxidant vitamins in preventing preeclampsia and serious complications of pregnancy-associated hypertension (PAH). This is a secondary analysis of a randomized controlled trial in which 10,154 low-risk women received daily vitamin C and E, or placebo, from 9-16 weeks gestation until delivery. Hp phenotype was determined in the study prediction cohort (n = 2,393) and a case-control cohort (703 cases, 1,406 controls). The primary outcome was severe PAH, or mild or severe PAH with elevated liver enzymes, elevated serum creatinine, thrombocytopenia, eclampsia, fetal growth restriction, medically indicated preterm birth or perinatal death. Preeclampsia was a secondary outcome. Odds ratios were estimated by logistic regression. Sampling weights were used to reduce bias from an overrepresentation of women with preeclampsia or the primary outcome. There was no relationship between Hp phenotype and the primary outcome or preeclampsia in Hispanic, white/other or black women. Vitamin supplementation did not reduce the risk of the primary outcome or preeclampsia in women of any phenotype. Supplementation increased preeclampsia risk (odds ratio 3.30; 95% confidence interval 1.61-6.82, p<0.01) in Hispanic Hp 2-2 women. Hp phenotype does not influence preeclampsia risk, or identify a subset of women who may benefit from vitamin C and E supplementation to prevent preeclampsia. C1 [Weissgerber, Tracey L.] Mayo Clin, Div Nephrol & Hypertens, Rochester, MN 55905 USA. [Gandley, Robin E.] Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USA. [McGee, Paula L.] George Washington Univ, Ctr Biostat, Washington, DC USA. [Spong, Catherine Y.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Bethesda, MD USA. [Myatt, Leslie] Univ Cincinnati, Dept Obstet & Gynecol, Cincinnati, OH USA. [Leveno, Kenneth J.] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA. [Thorp, John M., Jr.] Univ N Carolina, Chapel Hill, NC USA. [Mercer, Brian M.] Case Western Reserve Univ, Metrohlth Med Ctr, Cleveland, OH USA. [Peaceman, Alan M.] Northwestern Univ, Chicago, IL 60611 USA. [Ramin, Susan M.] Univ Texas Hlth Sci Ctr Houston, Childrens Mem Hermann Hosp, Houston, TX USA. [Carpenter, Marshall W.] Brown Univ, Providence, RI 02912 USA. [Samuels, Philip] Ohio State Univ, Columbus, OH 43210 USA. [Sciscione, Anthony] Drexel Univ, Philadelphia, PA 19104 USA. [Harper, Margaret] Wake Forest Univ Hlth Sci, Winston Salem, NC USA. [Tolosa, Jorge E.] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Saade, George] Univ Texas Med Branch, Galveston, TX 77555 USA. [Sorokin, Yoram] Wayne State Univ, Detroit, MI USA. RP Weissgerber, TL (reprint author), Mayo Clin, Div Nephrol & Hypertens, Rochester, MN 55905 USA. EM weissgerber.tracey@mayo.edu RI Weissgerber, Tracey/D-2324-2014; Samuels, Philip/E-4011-2011; OI Weissgerber, Tracey/0000-0002-7490-2600; Peaceman, Alan/0000-0002-4515-4850 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) [P01 HD030367, HD34208, HD27869, HD40485, HD40560, HD40544, HD34116, HD40512, HD21410, HD40545, HD40500, HD27915, HD34136, HD27860, HD53118, HD53097, HD27917, HD36801]; National Heart, Lung, and Blood Institute; National Center for Research Resources [M01 RR00080, UL1 RR024153, UL1 RR024989]; Canadian Institute of Health Research Fellowship; Amy Roberts Health Promotion Research Award; Office of Women's Health Research (Building Interdisciplinary Careers in Women's Health award) [K12HD065987] FX The project was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD: http://www.nichd.nih.gov/Pages/index.aspx) (P01 HD030367, HD34208, HD27869, HD40485, HD40560, HD40544, HD34116, HD40512, HD21410, HD40545, HD40500, HD27915, HD34136, HD27860, HD53118, HD53097, HD27917, and HD36801); the National Heart, Lung, and Blood Institute; and the National Center for Research Resources (M01 RR00080, UL1 RR024153, UL1 RR024989). Its contents do not necessarily represent the official view of NICHD, NHLBI, NCRR or NIH. As the program scientist, Catherine Y. Spong (NICHD) participated in the design of the study and development of the protocol and data forms; monitored the collection of the data; participated in the analysis, review, writing and critique of the manuscript and approval of its final version. She also oversees funding for all MFMU Network study sites and trials. Tracey Weissgerber was supported by a Canadian Institute of Health Research Fellowship, an Amy Roberts Health Promotion Research Award, and the Office of Women's Health Research (Building Interdisciplinary Careers in Women's Health award K12HD065987). NR 48 TC 9 Z9 10 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 3 PY 2013 VL 8 IS 4 AR e60479 DI 10.1371/journal.pone.0060479 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 143BA UT WOS:000318840100077 PM 23573260 ER PT J AU Giessing, C Thiel, CM Alexander-Bloch, AF Patel, AX Bullmore, ET AF Giessing, Carsten Thiel, Christiane M. Alexander-Bloch, Aaron F. Patel, Ameera X. Bullmore, Edward T. TI Human Brain Functional Network Changes Associated with Enhanced and Impaired Attentional Task Performance SO JOURNAL OF NEUROSCIENCE LA English DT Article ID GLOBAL WORKSPACE THEORY; SMALL-WORLD NETWORKS; AGE-RELATED-CHANGES; WORKING-MEMORY; NICOTINE GUM; VISUOSPATIAL ATTENTION; COGNITIVE NEUROSCIENCE; CIGARETTE-SMOKING; CONNECTIVITY MRI; PARIETAL CORTEX AB How is the cognitive performance of the human brain related to its topological and spatial organization as a complex network embedded in anatomical space? To address this question, we used nicotine replacement and duration of attentionally demanding task performance (time-on-task), as experimental factors expected, respectively, to enhance and impair cognitive function. We measured resting-state fMRI data, performance and brain activation on a go/no-go task demanding sustained attention, and subjective fatigue in n = 18 healthy, briefly abstinent, cigarette smokers scanned repeatedly in a placebo-controlled, crossover design. We tested the main effects of drug (placebo vs Nicorette gum) and time-on-task on behavioral performance and brain functional network metrics measured in binary graphs of 477 regional nodes (efficiency, measure of integrative topology; clustering, a measure of segregated topology; and the Euclidean physical distance between connected nodes, a proxy marker of wiring cost). Nicotine enhanced attentional task performance behaviorally and increased efficiency, decreased clustering, and increased connection distance of brain networks. Greater behavioral benefits of nicotine were correlated with stronger drug effects on integrative and distributed network configuration and with greater frequency of cigarette smoking. Greater time-on-task had opposite effects: it impaired attentional accuracy, decreased efficiency, increased clustering, and decreased connection distance of networks. These results are consistent with hypothetical predictions that superior cognitive performance should be supported by more efficient, integrated (high capacity) brain network topology at greater connection distance (high cost). They also demonstrate that brain network analysis can provide novel and theoretically principled pharmacodynamic biomarkers of pro-cognitive drug effects in humans. C1 [Giessing, Carsten; Alexander-Bloch, Aaron F.; Patel, Ameera X.; Bullmore, Edward T.] Univ Cambridge, Behav & Clin Neurosci Inst, Cambridge CB2 3EB, England. [Giessing, Carsten; Thiel, Christiane M.] Carl von Ossietzky Univ Oldenburg, Inst Psychol, Biol Psychol Lab, D-26129 Oldenburg, Germany. [Alexander-Bloch, Aaron F.] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. [Bullmore, Edward T.] GlaxoSmithKline, Addenbrookes Ctr Clin Invest, Clin Unit Cambridge, Cambridge CB2 0GG, England. [Bullmore, Edward T.] Cambridgeshire & Peterborough Natl Hlth Serv Fdn, Cambridge CB21 5EF, England. RP Giessing, C (reprint author), Carl von Ossietzky Univ Oldenburg, Inst Psychol, Biol Psychol Lab, D-26111 Oldenburg, Germany. EM carsten.giessing@uni-oldenburg.de RI Bullmore, Edward/C-1706-2012; OI Bullmore, Edward/0000-0002-8955-8283; Alexander-Bloch, Aaron/0000-0001-6554-1893 FU Deutsche Forschungsgemeinschaft [GI 682/2-1]; Medical Research Council; Wellcome Trust; National Institutes of Health Cambridge Graduate Partnership Program; Wellcome Trust/GlaxoSmithKline Translational Medicine and Therapeutics program; MB/PhD program in the School of Clinical Medicine, University of Cambridge FX This study was supported by Deutsche Forschungsgemeinschaft Grant GI 682/2-1 (C.G.). The Behavioural and Clinical Neuroscience Institute is supported by the Medical Research Council and the Wellcome Trust. A.F.A.-B. was supported by the National Institutes of Health Cambridge Graduate Partnership Program. A. X. P. was supported by the Wellcome Trust/GlaxoSmithKline Translational Medicine and Therapeutics program and the MB/PhD program in the School of Clinical Medicine, University of Cambridge. We thank Drs. Andrew Zalesky, Alex Fornito, and David Meunier for their helpful advice and McNeil (Helsingborg, Sweden) for providing the placebo. NR 90 TC 33 Z9 33 U1 3 U2 34 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD APR 3 PY 2013 VL 33 IS 14 BP 5903 EP 5914 DI 10.1523/JNEUROSCI.4854-12.2013 PG 12 WC Neurosciences SC Neurosciences & Neurology GA 120QP UT WOS:000317186800004 PM 23554472 ER PT J AU McDonald, CJ Vreeman, DJ Abhyankar, S AF McDonald, Clement J. Vreeman, Daniel J. Abhyankar, Swapna TI Comment on "Time to Integrate Clinical and Research Informatics" SO SCIENCE TRANSLATIONAL MEDICINE LA English DT Letter C1 [McDonald, Clement J.; Abhyankar, Swapna] NIH, Lister Hill Natl Ctr Biomed Commun, Natl Lib Med, Bethesda, MD 20894 USA. [Vreeman, Daniel J.] Indiana Univ Sch Med, Indianapolis, IN 46202 USA. [Vreeman, Daniel J.] Regenstrief Inst Inc, Indianapolis, IN 46202 USA. RP Abhyankar, S (reprint author), NIH, Lister Hill Natl Ctr Biomed Commun, Natl Lib Med, Bethesda, MD 20894 USA. EM swapna.abhyankar@nih.gov FU Intramural NIH HHS; PHS HHS [HHSN2762008000006C] NR 9 TC 1 Z9 1 U1 2 U2 5 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 1946-6234 J9 SCI TRANSL MED JI Sci. Transl. Med. PD APR 3 PY 2013 VL 5 IS 179 AR 179le1 DI 10.1126/scitranslmed.3005700 PG 2 WC Cell Biology; Medicine, Research & Experimental SC Cell Biology; Research & Experimental Medicine GA 118PF UT WOS:000317037000002 PM 23552367 ER PT J AU Kutty, RK Nagineni, CN Samuel, W Vijayasarathy, C Jaworski, C Duncan, T Cameron, JE Flemington, EK Hooks, JJ Redmond, TM AF Kutty, R. Krishnan Nagineni, Chandrasekharam N. Samuel, William Vijayasarathy, Camasamudram Jaworski, Cynthia Duncan, Todd Cameron, Jennifer E. Flemington, Erik K. Hooks, John J. Redmond, T. Michael TI Differential regulation of microRNA-146a and microRNA-146b-5p in human retinal pigment epithelial cells by interleukin-1 beta, tumor necrosis factor-alpha, and interferon-gamma SO MOLECULAR VISION LA English DT Article ID NF-KAPPA-B; MACULAR DEGENERATION; RHEUMATOID-ARTHRITIS; GENE-EXPRESSION; INFLAMMATORY MEDIATORS; SIGNALING PROTEINS; SYNOVIAL TISSUE; RPE CELLS; MIR-146A; PATHWAY AB Purpose: The inflammatory response of the retinal pigment epithelium (RPE) is implicated in the pathogenesis of age-related macular degeneration. The microRNAs miR-146a and miR-146b-5p can regulate the inflammatory process by attenuating cytokine signaling via the nuclear factor-kappa B pathway. The aim of the present study is to investigate the expression of miR-146a and miR-146b-5p in human RPE cells and their response to proinflammatory cytokines. Methods: Confluent cultures of RPE cells established from adult human donor eyes were treated with the proinflammatory cytokines interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1 beta. The expression of microRNAs was analyzed by real-time PCR using total RNA fraction. The retinal pigment epithelial cell line ARPE-19 was employed to analyze the promoter activity of the genes encoding miR-146a and miR-146b-5p. STAT1-binding activity of oligonucleotides was analyzed by electrophoretic mobility shift assay. ARPE-19 cells were transiently transfected with miR-146a and miR-146b-5p mimics for the analysis of IRAK1 expression by western immunoblotting. Results: Real-time PCR analysis showed that miR-146a and 146b-5p are expressed in RPE cells. The cells responded to proinflammatory cytokines (IFN-gamma + TNF-alpha + IL-1 beta) by highly increasing the expression of both miR-146a and miR-146b-5p. This was associated with an increase in the expression of transcripts for CCL2, CCL5, CXCL9, CXCL10, and IL-6, and a decrease in that for HMOX1. The miR-146a induction was more dependent on IL-1 beta, since its omission from the cytokine mix resulted in a greatly reduced response. Similarly, the induction of miR-146b-5p was more dependent on IFN-gamma, since its omission from the cytokine mix minimized the effect. In addition, the increase in MIR146B promoter activity by the cytokine mix was effectively blocked by JAK inhibitor 1, a known inhibitor of the JAK/STAT signaling pathway. The expression of IRAK1 protein was decreased when ARPE-19 cells were transiently transfected with either miR-146a mimic or miR-146b-5p mimic. Conclusions: Our results clearly show that both miR-146a and miR-146b-5p are expressed in human RPE cells in culture and their expression is highly induced by proinflammatory cytokines (IFN-gamma + TNF-alpha + IL-1 beta). The induction of miR-146a showed a dependency on IL-1 beta, while that of miR-146b-5p on IFN-gamma. Our results show for the first time that miR-146b-5p expression is regulated by IFN-gamma, potentially via the JAK/STAT pathway. These two microRNAs could play a role in inflammatory processes underlying age-related macular degeneration or other retinal degenerative diseases through their ability to negatively regulate the nuclear factor-kappa B pathway by targeting the expression of IRAK1. C1 [Kutty, R. Krishnan; Samuel, William; Jaworski, Cynthia; Duncan, Todd; Redmond, T. Michael] NEI, Lab Retinal Cell & Mol Biol, NIH, Bethesda, MD 20892 USA. [Nagineni, Chandrasekharam N.; Hooks, John J.] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Vijayasarathy, Camasamudram] Natl Inst Deafness & Other Commun Disorders, NIH, Bethesda, MD USA. [Cameron, Jennifer E.; Flemington, Erik K.] Tulane Univ, Tulane Canc Ctr, New Orleans, LA 70118 USA. [Cameron, Jennifer E.] Louisiana State Univ, Hlth Sci Ctr, New Orleans, LA USA. [Cameron, Jennifer E.] Louisiana State Univ, Hlth Sci Ctr, Stanley S Scott Canc Ctr, New Orleans, LA USA. RP Kutty, RK (reprint author), NEI, Lab Retinal Cell & Mol Biol, NIH, Bldg 6,Room 112,6 Ctr Dr,MSC 0608, Bethesda, MD 20892 USA. EM kuttyk@nei.nih.gov OI Redmond, T. Michael/0000-0002-1813-5291 FU Intramural Research Program of the National Eye Institute, NIH [Z01EY000444] FX This study was supported by the Intramural Research Program of the National Eye Institute, NIH (Z01EY000444). NR 48 TC 33 Z9 34 U1 1 U2 11 PU MOLECULAR VISION PI ATLANTA PA C/O JEFF BOATRIGHT, LAB B, 5500 EMORY EYE CENTER, 1327 CLIFTON RD, N E, ATLANTA, GA 30322 USA SN 1090-0535 J9 MOL VIS JI Mol. Vis. PD APR 3 PY 2013 VL 19 BP 737 EP 750 PG 14 WC Biochemistry & Molecular Biology; Ophthalmology SC Biochemistry & Molecular Biology; Ophthalmology GA 118BM UT WOS:000316997400001 PM 23592910 ER PT J AU Sun, SY Xiang, Y Akahata, W Holdaway, H Pal, P Zhang, XZ Diamond, MS Nabel, GJ Rossmann, MG AF Sun, Siyang Xiang, Ye Akahata, Wataru Holdaway, Heather Pal, Pankaj Zhang, Xinzheng Diamond, Michael S. Nabel, Gary J. Rossmann, Michael G. TI Structural analyses at pseudo atomic resolution of Chikungunya virus and antibodies show mechanisms of neutralization SO ELIFE LA English DT Article ID EQUINE ENCEPHALITIS-VIRUS; SEMLIKI-FOREST-VIRUS; E2 ENVELOPE GLYCOPROTEIN; SINDBIS-VIRUS; CRYOELECTRON MICROSCOPY; NUCLEOCAPSID PROTEIN; ELECTRON-MICROSCOPY; HEPARAN-SULFATE; CORE PROTEIN; CRYO-EM AB A 5.3 angstrom resolution, cryo-electron microscopy (cryoEM) map of Chikungunya virus-like particles (VLPs) has been interpreted using the previously published crystal structure of the Chikungunya E1-E2 glycoprotein heterodimer. The heterodimer structure was divided into domains to obtain a good fit to the cryoEM density. Differences in the T = 4 quasi-equivalent heterodimer components show their adaptation to different environments. The spikes on the icosahedral 3-fold axes and those in general positions are significantly different, possibly representing different phases during initial generation of fusogenic E1 trimers. CryoEM maps of neutralizing Fab fragments complexed with VLPs have been interpreted using the crystal structures of the Fab fragments and the VLP structure. Based on these analyses the CHK-152 antibody was shown to stabilize the viral surface, hindering the exposure of the fusion-loop, likely neutralizing infection by blocking fusion. The CHK-9, m10 and m242 antibodies surround the receptor-attachment site, probably inhibiting infection by blocking cell attachment. C1 [Sun, Siyang; Xiang, Ye; Holdaway, Heather; Zhang, Xinzheng; Rossmann, Michael G.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Akahata, Wataru; Nabel, Gary J.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. [Pal, Pankaj; Diamond, Michael S.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63130 USA. [Pal, Pankaj; Diamond, Michael S.] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63130 USA. [Pal, Pankaj; Diamond, Michael S.] Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63130 USA. [Pal, Pankaj; Diamond, Michael S.] Washington Univ, Sch Med, Dept Immunol, St Louis, MO 63130 USA. RP Rossmann, MG (reprint author), Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. EM mr@purdue.edu RI Zhang , Xinzheng/E-6339-2013 FU NIAID NIH HHS [AI095366, AI104545, R01 AI089591, R01 AI095366] NR 87 TC 28 Z9 28 U1 0 U2 4 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD APR 2 PY 2013 VL 2 AR e00435 DI 10.7554/eLife.00435 PG 27 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 274NQ UT WOS:000328613600003 PM 23577234 ER PT J AU Elinoff, JM Rame, JE Forfia, PR Hall, MK Sun, J Gharib, AM Abd-Elmoniem, K Graninger, G Harper, B Danner, RL Solomon, MA AF Elinoff, Jason M. Rame, J. Eduardo Forfia, Paul R. Hall, Mary K. Sun, Junfeng Gharib, Ahmed M. Abd-Elmoniem, Khaled Graninger, Grace Harper, Bonnie Danner, Robert L. Solomon, Michael A. TI A pilot study of the effect of spironolactone therapy on exercise capacity and endothelial dysfunction in pulmonary arterial hypertension: study protocol for a randomized controlled trial SO TRIALS LA English DT Article DE Magnetic resonance imaging; Microarray; Mineralocorticoid receptor antagonist; Neurohormonal axis; Pulmonary arterial hypertension; Right ventricular function; Vascular inflammation ID EXTENDED CLINICAL-EXPERIENCE; GENE MICROARRAY ANALYSIS; CHRONIC HEART-FAILURE; 6-MINUTE WALK TEST; MINERALOCORTICOID RECEPTOR; GADOPENTETATE DIMEGLUMINE; PROINFLAMMATORY CYTOKINES; VENTRICULAR DYSFUNCTION; NONINVASIVE ASSESSMENT; INHIBITS PRODUCTION AB Background: Pulmonary arterial hypertension is a rare disorder associated with poor survival. Endothelial dysfunction plays a central role in the pathogenesis and progression of pulmonary arterial hypertension. Inflammation appears to drive this dysfunctional endothelial phenotype, propagating cycles of injury and repair in genetically susceptible patients with idiopathic and disease-associated pulmonary arterial hypertension. Therapy targeting pulmonary vascular inflammation to interrupt cycles of injury and repair and thereby delay or prevent right ventricular failure and death has not been tested. Spironolactone, a mineralocorticoid and androgen receptor antagonist, has been shown to improve endothelial function and reduce inflammation. Current management of patients with pulmonary arterial hypertension and symptoms of right heart failure includes use of mineralocorticoid receptor antagonists for their diuretic and natriuretic effects. We hypothesize that initiating spironolactone therapy at an earlier stage of disease in patients with pulmonary arterial hypertension could provide additional benefits through anti-inflammatory effects and improvements in pulmonary vascular function. Methods/Design: Seventy patients with pulmonary arterial hypertension without clinical evidence of right ventricular failure will be enrolled in a randomized, double-blinded, placebo-controlled trial to investigate the effect of early treatment with spironolactone on exercise capacity, clinical worsening and vascular inflammation in vivo. Our primary endpoint is change in placebo-corrected 6-minute walk distance at 24 weeks and the incidence of clinical worsening in the spironolactone group compared to placebo. At a two-sided alpha level of 0.05, we will have at least 84% power to detect an effect size (group mean difference divided by standard deviation) of 0.9 for the difference in the change of 6-minute walk distance from baseline between the two groups. Secondary endpoints include the effect of spironolactone on the change in placebo-corrected maximal oxygen consumption; plasma markers of vascular inflammation and peripheral blood mononuclear cell gene expression profiles; sympathetic nervous system activation, renin-angiotensin-aldosterone system activation and sex hormone metabolism; and right ventricular structure and function using echocardiography and novel high-resolution magnetic resonance imaging-based techniques. Safety and tolerability of spironolactone will be assessed with periodic monitoring for hyperkalemia and renal insufficiency as well as the incidence of drug discontinuation for untoward effects. C1 [Elinoff, Jason M.; Hall, Mary K.; Sun, Junfeng; Graninger, Grace; Harper, Bonnie; Danner, Robert L.; Solomon, Michael A.] NIH, Dept Crit Care Med, Mark O Hatfield Clin Res Ctr, Bethesda, MD 20892 USA. [Rame, J. Eduardo; Forfia, Paul R.] Hosp Univ Penn, Dept Med, Div Cardiovasc Med, Philadelphia, PA 19104 USA. [Gharib, Ahmed M.; Abd-Elmoniem, Khaled] NIDDKD, NIH, Biomed & Metab Imaging Branch, Bethesda, MD 20892 USA. [Solomon, Michael A.] NHLBI, NIH, Cardiovasc & Pulm Branch, Bethesda, MD 20892 USA. RP Elinoff, JM (reprint author), NIH, Dept Crit Care Med, Mark O Hatfield Clin Res Ctr, 10 Ctr Dr,Bldg 10,Room 2C145, Bethesda, MD 20892 USA. EM elinoffj@cc.nih.gov RI Gharib, Ahmed/O-2629-2016; Abd-Elmoniem, Khaled/B-9289-2008 OI Gharib, Ahmed/0000-0002-2476-481X; Abd-Elmoniem, Khaled/0000-0002-1001-1702 FU NIH Office of Research on Women's Health; Critical Care Medicine Department, NIH Clinical Center FX The funding for this study is from the NIH Office of Research on Women's Health (2011 Bench to Bedside Award; $270,000 over two years), as well as intramural funding from the Critical Care Medicine Department, NIH Clinical Center. We are grateful for the support and assistance of Judith Welsh (NIH Clinical Center biomedical librarian), Judith Starling (pharmacist) and the Pharmaceutical Development Section of the NIH Clinical Center Pharmacy. NR 78 TC 8 Z9 8 U1 1 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1745-6215 J9 TRIALS JI Trials PD APR 2 PY 2013 VL 14 AR 91 DI 10.1186/1745-6215-14-91 PG 16 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 145BD UT WOS:000318987000002 PM 23547564 ER PT J AU Middleton, KR Ward, MM Haaz, S Velummylum, S Fike, A Acevedo, AT Tataw-Ayuketah, G Dietz, L Mittleman, BB Wallen, GR AF Middleton, Kimberly R. Ward, Michael M. Haaz, Steffany Velummylum, Sinthujah Fike, Alice Acevedo, Ana T. Tataw-Ayuketah, Gladys Dietz, Laura Mittleman, Barbara B. Wallen, Gwenyth R. TI A pilot study of yoga as self-care for arthritis in minority communities SO HEALTH AND QUALITY OF LIFE OUTCOMES LA English DT Article DE Yoga; Complementary and alternative medicine; Minority; Osteoarthritis; Rheumatoid arthritis; Self-efficacy ID HEALTH INTERVIEW SURVEY; QUALITY-OF-LIFE; RHEUMATOID-ARTHRITIS; IYENGAR YOGA; OLDER-ADULTS; SALIVARY CORTISOL; PHYSICAL-ACTIVITY; OUTCOME MEASURES; REPORTED HEALTH; ELDERLY PERSONS AB Background: While arthritis is the most common cause of disability, non-Hispanic blacks and Hispanics experience worse arthritis impact despite having the same or lower prevalence of arthritis compared to non-Hispanic whites. People with arthritis who exercise regularly have less pain, more energy, and improved sleep, yet arthritis is one of the most common reasons for limiting physical activity. Mind-body interventions, such as yoga, that teach stress management along with physical activity may be well suited for investigation in both osteoarthritis and rheumatoid arthritis. Yoga users are predominantly white, female, and college educated. There are few studies that examine yoga in minority populations; none address arthritis. This paper presents a study protocol examining the feasibility and acceptability of providing yoga to an urban, minority population with arthritis. Methods/design: In this ongoing pilot study, a convenience sample of 20 minority adults diagnosed with either osteoarthritis or rheumatoid arthritis undergo an 8-week program of yoga classes. It is believed that by attending yoga classes designed for patients with arthritis, with racially concordant instructors; acceptability of yoga as an adjunct to standard arthritis treatment and self-care will be enhanced. Self-care is defined as adopting behaviors that improve physical and mental well-being. This concept is quantified through collecting patient-reported outcome measures related to spiritual growth, health responsibility, interpersonal relations, and stress management. Additional measures collected during this study include: physical function, anxiety/depression, fatigue, sleep disturbance, social roles, and pain; as well as baseline demographic and clinical data. Field notes, quantitative and qualitative data regarding feasibility and acceptability are also collected. Acceptability is determined by response/retention rates, positive qualitative data, and continuing yoga practice after three months. Discussion: There are a number of challenges in recruiting and retaining participants from a community clinic serving minority populations. Adopting behaviors that improve well-being and quality of life include those that integrate mental health (mind) and physical health (body). Few studies have examined offering integrative modalities to this population. This pilot was undertaken to quantify measures of feasibility and acceptability that will be useful when evaluating future plans for expanding the study of yoga in urban, minority populations with arthritis. C1 [Middleton, Kimberly R.; Velummylum, Sinthujah; Tataw-Ayuketah, Gladys; Wallen, Gwenyth R.] NIH, Ctr Clin, Dept Nursing, Bethesda, MD 20892 USA. [Ward, Michael M.; Fike, Alice] NIAMSD, NIH, Bethesda, MD 20892 USA. [Mittleman, Barbara B.] NIH, Off Sci Policy, Off Director, Bethesda, MD 20892 USA. [Acevedo, Ana T.; Dietz, Laura] NIH, Ctr Clin, Bethesda, MD 20892 USA. [Haaz, Steffany] Yoga Arthrit, Baltimore, MD USA. RP Middleton, KR (reprint author), NIH, Ctr Clin, Dept Nursing, 10 Ctr Dr, Bethesda, MD 20892 USA. EM middletonk@cc.nih.gov FU National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) FX The study was supported in part by the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). Many thanks to Regina Andrade, BA; Li Yang, MS; NIAMS Community Health Center staff; and research participants. NR 111 TC 2 Z9 3 U1 4 U2 41 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1477-7525 J9 HEALTH QUAL LIFE OUT JI Health Qual. Life Outcomes PD APR 2 PY 2013 VL 11 AR 55 DI 10.1186/1477-7525-11-55 PG 14 WC Health Care Sciences & Services; Health Policy & Services SC Health Care Sciences & Services GA 134TS UT WOS:000318238200001 PM 23548052 ER PT J AU Jao, CC Ragusa, MJ Stanley, RE Hurley, JH AF Jao, Christine C. Ragusa, Michael J. Stanley, Robin E. Hurley, James H. TI A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE protein crystallography; protein degradation; protein structure; yeast genetics ID SPINDLE ASSEMBLY CHECKPOINT; SACCHAROMYCES-CEREVISIAE; MONITORING AUTOPHAGY; KINASE COMPLEX; YEAST; PROTEIN; MAD2; BIOGENESIS; MACHINERY; CYTOPLASM AB Autophagy-related 13 (Atg13) is a key early-acting factor in autophagy and the major locus for nutrient-dependent regulation of autophagy by Tor. The 2.3-angstrom resolution crystal structure of the N-terminal domain of Atg13 reveals a previously unidentified HORMA (Hop1p, Rev1p and Mad2) domain similar to that of the spindle checkpoint protein Mad2. Mad2 has two different stable conformations, O-Mad2 and C-Mad2, and the Atg13 HORMA structure corresponds to the C-Mad2 state. The Atg13 HORMA domain is required for autophagy and for recruitment of the phosphatidylinositol (PI) 3-kinase subunit Atg14 but is not required for Atg1 interaction or Atg13 recruitment to the preautophagosomal structure. The Atg13 HORMA structure reveals a pair of conserved Arg residues that constitute a putative phosphate sensor. One of the Arg residues is in the region corresponding to the "safety belt" conformational switch of Mad2, suggesting conformational regulation of phosphate binding. These two Arg residues are essential for autophagy, suggesting that the Atg13 HORMA domain could function as a phosphoregulated conformational switch. C1 [Jao, Christine C.; Ragusa, Michael J.; Stanley, Robin E.; Hurley, James H.] NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. RP Hurley, JH (reprint author), NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. EM james.hurley@nih.gov FU US Department of Energy, Office of Science, Office of Basic Energy Sciences [W-31-109-Eng-38]; National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of General Medical Sciences; Damon Runyon Cancer Research Fellowship; Ruth Kirschtein National Research Service Award Fellowship [GM099319] FX We thank A. Toulmay and W. Prinz for advice, N. Noinaj and P. Lukacik for data collection at the Diamond Light Source, and D. J. Klionksy and R. Youle for yeast strains. Crystallographic data were collected at Southeast Regional Collaborative Access Team 22-ID beamline at the Advanced Photon Source, Argonne National Laboratory and at the Diamond Light Source, Oxfordshire, United Kingdom. Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. W-31-109-Eng-38. This work was supported by the Intramural Program of the National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (J. H. H.), a fellowship from the National Institute of General Medical Sciences Postdoctoral Research Associate Program (C. C. J.), a Damon Runyon Cancer Research Fellowship (R. E. S.), and by Ruth Kirschtein National Research Service Award Fellowship GM099319 (to M.J.R.). NR 34 TC 41 Z9 42 U1 2 U2 7 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 2 PY 2013 VL 110 IS 14 BP 5486 EP 5491 DI 10.1073/pnas.1220306110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 131ZP UT WOS:000318037800058 PM 23509291 ER PT J AU Tregoning, JS Wang, BL McDonald, JU Yamaguchi, Y Harker, JA Goritzka, M Johansson, C Bukreyev, A Collins, PL Openshaw, PJ AF Tregoning, John S. Wang, Belinda Lei McDonald, Jacqueline U. Yamaguchi, Yuko Harker, James A. Goritzka, Michelle Johansson, Cecilia Bukreyev, Alexander Collins, Peter L. Openshaw, Peter J. TI Neonatal antibody responses are attenuated by interferon-gamma produced by NK and T cells during RSV infection SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE viral infection; bronchiolitis; lung infection ID RESPIRATORY SYNCYTIAL VIRUS; VACCINE CANDIDATE; IMMUNE-RESPONSE; VIRAL-INFECTION; B-CELLS; MICE; AGE; REINFECTION; EXPRESSION; INFANTS AB Respiratory syncytial virus (RSV) infects most children in the first year of life and is a major single cause of hospitalization in infants and young children. There is no effective vaccine, and antibody generated by primary neonatal infection is poorly protective against reinfection even with antigenically homologous viral strains. Studying the immunological basis of these observations in neonatal mice, we found that antibody responses to infection were low and unaffected by CD4 depletion, in contrast with adult mice, which had stronger CD4-dependent antibody responses. Natural killer cell depletion or codepletion of CD4(+) and CD8(+) cells during neonatal RSV infection caused a striking increase in anti-RSV antibody titer. These cells are major sources of the cytokine IFN-gamma, and blocking IFN-gamma also enhanced RSV-specific antibody responses in neonates. In addition, infection with a recombinant RSV engineered to produce IFN-gamma reduced antibody titer, confirming that IFN-gamma plays a pivotal role in inhibition of antibody responses after neonatal infection. These unexpected findings show that the induction of a strong cellular immune response may limit antibody responses in early life and that vaccines that induce IFN-gamma-secreting cells might, in some situations, be less protective than those that do not. C1 [Tregoning, John S.; McDonald, Jacqueline U.] Univ London Imperial Coll Sci Technol & Med, Mucosal Infect & Immun Grp, Infect Dis Sect, Dept Med, London W2 1PG, England. [Tregoning, John S.; Wang, Belinda Lei; Yamaguchi, Yuko; Harker, James A.; Goritzka, Michelle; Johansson, Cecilia; Openshaw, Peter J.] Univ London Imperial Coll Sci Technol & Med, Dept Resp Med, Ctr Resp Infect, London W2 1PG, England. [Tregoning, John S.; Wang, Belinda Lei; Yamaguchi, Yuko; Harker, James A.; Goritzka, Michelle; Johansson, Cecilia; Openshaw, Peter J.] Univ London Imperial Coll Sci Technol & Med, MRC, London W2 1PG, England. [Tregoning, John S.; Wang, Belinda Lei; Yamaguchi, Yuko; Harker, James A.; Goritzka, Michelle; Johansson, Cecilia; Openshaw, Peter J.] Univ London Imperial Coll Sci Technol & Med, Asthma UK Ctr Allerg Mechanisms Asthma, Natl Heart & Lung Inst, London W2 1PG, England. [Tregoning, John S.] St Georges Univ London, Ctr Infect & Immun, London SW17 0RE, England. [Bukreyev, Alexander; Collins, Peter L.] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. RP Tregoning, JS (reprint author), Univ London Imperial Coll Sci Technol & Med, Mucosal Infect & Immun Grp, Infect Dis Sect, Dept Med, London W2 1PG, England. EM john.tregoning@imperial.ac.uk; p.openshaw@imperial.ac.uk OI Tregoning, John/0000-0001-8093-8741 FU Royal Society; St. George's University of London; Wellcome Trust [071381/Z/03/Z]; Medical Research Council [G0800311]; National Institute of Allergy and Infectious Diseases, National Institutes of Health FX The authors thank Steve Cobbold for depleting antibodies, Anthony Warrens for the FasL-/- deficient mice, the staff of St. Mary's and St. George's University of London Central Biomedical Services for their support with in vivo studies, and Dr. Paul McKay (Imperial College London) for assistance with the development of the quantitative RSV IgG ELISA. In particular, the authors acknowledge the longstanding support of Prof. Ita Askonas and all her invaluable advice. This work was funded by a Research Grant from The Royal Society (to J.S.T.), research funding from St. George's University of London (to J.S.T.), Wellcome Trust Programme Grant 071381/Z/03/Z (to P.J.O.), a Medical Research Council Career Development Award Grant G0800311 (to C.J.), and an intramural research program, National Institute of Allergy and Infectious Diseases, National Institutes of Health (to P. L. C. and A.B.). NR 56 TC 15 Z9 15 U1 0 U2 12 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 2 PY 2013 VL 110 IS 14 BP 5576 EP 5581 DI 10.1073/pnas.1214247110 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 131ZP UT WOS:000318037800073 PM 23509276 ER PT J AU Wang, XX Towers, S Panchanathan, S Chowell, G AF Wang, Xiaoxia Towers, Sherry Panchanathan, Sarada Chowell, Gerardo TI A Population Based Study of Seasonality of Skin and Soft Tissue Infections: Implications for the Spread of CA-MRSA SO PLOS ONE LA English DT Article ID RESISTANT STAPHYLOCOCCUS-AUREUS; ACQUIRED METHICILLIN-RESISTANT; EMERGENCY-DEPARTMENT; SPECTRAL-ANALYSIS; UNITED-STATES; SPACED DATA; CARRIAGE; CHILDREN; HOSPITALIZATIONS; PREVALENCE AB Methicillin resistant Staphylococcus aureus (MRSA) is currently a major cause of skin and soft tissue infections (SSTI) in the United States. Seasonal variation of MRSA infections in hospital settings has been widely observed. However, systematic time-series analysis of incidence data is desirable to understand the seasonality of community acquired (CA)-MRSA infections at the population level. In this paper, using data on monthly SSTI incidence in children aged 0-19 years and enrolled in Medicaid in Maricopa County, Arizona, from January 2005 to December 2008, we carried out time-series and nonlinear regression analysis to determine the periodicity, trend, and peak timing in SSTI incidence in children at different age: 0-4 years, 5-9 years, 10-14 years, and 15-19 years. We also assessed the temporal correlation between SSTI incidence and meteorological variables including average temperature and humidity. Our analysis revealed a strong annual seasonal pattern of SSTI incidence with peak occurring in early September. This pattern was consistent across age groups. Moreover, SSTIs followed a significantly increasing trend over the 4-year study period with annual incidence increasing from 3.36% to 5.55% in our pediatric population of approximately 290,000. We also found a significant correlation between the temporal variation in SSTI incidence and mean temperature and specific humidity. Our findings could have potential implications on prevention and control efforts against CA-MRSA. C1 [Wang, Xiaoxia; Towers, Sherry; Chowell, Gerardo] Arizona State Univ, Math Computat & Modeling Sci Ctr, Sch Human Evolut & Social Change, Tempe, AZ 85287 USA. [Panchanathan, Sarada] Maricopa Integrated Hlth Syst, Dept Pediat, Phoenix, AZ USA. [Panchanathan, Sarada] Arizona State Univ, Dept Biomed Informat, Tempe, AZ USA. [Chowell, Gerardo] NIH, Div Epidemiol & Populat Studies, Fogarty Int Ctr, Bethesda, MD 20892 USA. RP Wang, XX (reprint author), Arizona State Univ, Math Computat & Modeling Sci Ctr, Sch Human Evolut & Social Change, Tempe, AZ 85287 USA. EM xwang248@asu.edu RI Chowell, Gerardo/F-5038-2012 OI Chowell, Gerardo/0000-0003-2194-2251 FU Arizona Biomedical Research Commission [1216] FX This work was supported by the Arizona Biomedical Research Commission (http://azdhs.gov/biomedical/) with Commission Contract No. 1216. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 31 TC 14 Z9 14 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 2 PY 2013 VL 8 IS 4 AR e60872 DI 10.1371/journal.pone.0060872 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 127RY UT WOS:000317717300154 PM 23565281 ER PT J AU Wohlfarth, A Scheidweiler, KB Chen, XH Liu, HF Huestis, MA AF Wohlfarth, Ariane Scheidweiler, Karl B. Chen, Xiaohong Liu, Hua-fen Huestis, Marilyn A. TI Qualitative Confirmation of 9 Synthetic Cannabinoids and 20 Metabolites in Human Urine Using LC-MS/MS and Library Search SO ANALYTICAL CHEMISTRY LA English DT Article ID CHROMATOGRAPHY-MASS SPECTROMETRY; QUANTITATIVE MEASUREMENT; SMOKING MIXTURES; JWH-018; IDENTIFICATION; DRUGS; BLOOD; HYDROLYSIS; GAS AB Introduction: Synthetic cannabinoids are an emerging illicit drug class. The variety of available substances is large and ever-changing, making it difficult for laboratories to remain current. We present a qualitative LC-MS/MS method identifying urinary metabolites of JWH-018, JWH-073, JWH-081, JWH-122, JWH-200, JWH-210, JWH-250, RCS-4, and AM2201 and the parent compounds JWH-018, JWH-073, JWH-081, JWH-122, JWH-210, JWH-250, RCS-4, AM2201, and MAM2201. Methods: After enzymatic hydrolysis, urinary proteins were precipitated with acetonitrile. Chromatography utilized a 10 min gradient on a Kinetex XII-C18 column with 0.1% formic acid in water and acetonitrile. Scheduled multiple reaction monitoring "survey scans" were followed by information-dependent acquisition-enhanced product ion scan experiments on an ABSciex 5500 QTRAP mass spectrometer. Analytes were identified by software-assisted library searching against reference spectra. Results: The method was fully validated, including proof of selectivity (no exogenous or endogenous interferences were observed), assessment of matrix effects (95-122%) and recovery (53-95%), determination of limits of detection (0.5-10 ng/mL), carry-over studies (thresholds between 100 and 1000 ng/mL), and determination of autosampler stability (samples were stable for at least 3 days). Hydrolysis efficiency was thoroughly investigated for a wide range of glucuronides and for the reference standard, JWH-018 5-hydroxypentyl glucuronide C1 [Wohlfarth, Ariane; Scheidweiler, Karl B.; Huestis, Marilyn A.] NIDA, Intramural Res Program, NIH, Baltimore, MD 21224 USA. [Chen, Xiaohong; Liu, Hua-fen] ABSciex, Foster City, CA 94404 USA. RP Huestis, MA (reprint author), NIDA, IRP, NIH, Biomed Res Ctr, 251 Bayview Blvd,Suite 200,Room 05A-721, Baltimore, MD 21224 USA. EM mhuestis@intra.nida.nih.gov FU National Institute on Drug Abuse, and National Institutes of Health FX This research was funded by the Intramural Research Program, National Institute on Drug Abuse, and National Institutes of Health. NR 31 TC 68 Z9 70 U1 9 U2 63 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD APR 2 PY 2013 VL 85 IS 7 BP 3730 EP 3738 DI 10.1021/ac3037365 PG 9 WC Chemistry, Analytical SC Chemistry GA 120MA UT WOS:000317173200040 PM 23458260 ER PT J AU Tan, ML Cendagorta, JR Ichiye, T AF Tan, Ming-Liang Cendagorta, Joseph R. Ichiye, Toshiko TI Effects of Microcomplexity on Hydrophobic Hydration in Amphiphiles SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID ALCOHOL-WATER; LIQUID WATER; TEMPERATURE; DEPENDENCE; METHANOL; MODEL AB Hydrophobic hydration is critical in biology as well as many industrial processes. Here, computer simulations of ethanol/water mixtures show that a three-stage mechanism of dehydration of ethanol explains the anomalous concentration dependence of the thermodynamic partial molar volumes, as well as recent data from neutron diffraction and Raman scattering. Moreover, the simulations show that the breakdown of hydrophobic hydration shells, whose structure is determined by the unique molecular properties of water, is caused by the microcomplexity of the environment and may be representative of early events in protein folding and structure stabilization in aqueous solutions. C1 [Tan, Ming-Liang; Cendagorta, Joseph R.; Ichiye, Toshiko] Georgetown Univ, Dept Chem, Washington, DC 20057 USA. [Ichiye, Toshiko] NHLBI, Lab Computat Biol, NIH, Bethesda, MD 20892 USA. RP Ichiye, T (reprint author), Georgetown Univ, Dept Chem, Washington, DC 20057 USA. EM ti9@georgetown.edu FU National Science Foundation [CHE-1158267]; McGowan Foundation FX We are grateful to the National Science Foundation (grant number CHE-1158267, T.I.) and to the McGowan Foundation (T.I.) for the support of this work. We also thank Benjamin T. Miller and Bernard R. Brooks for assistance in implementing the code, Peter J. Rossky and Sergio A. Hassan for insightful discussions, and Alan K. Soper for providing the neutron diffraction data for the aqueous solutions of methanol and ethanol. NR 29 TC 12 Z9 12 U1 3 U2 36 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD APR 2 PY 2013 VL 135 IS 13 BP 4918 EP 4921 DI 10.1021/ja312504q PG 4 WC Chemistry, Multidisciplinary SC Chemistry GA 121QM UT WOS:000317259300005 PM 23506339 ER PT J AU Emileh, A Tuzer, F Yeh, H Umashankara, M Moreira, DRM LaLonde, JM Bewley, CA Abrams, CF Chaiken, IM AF Emileh, Ali Tuzer, Ferit Yeh, Herman Umashankara, Muddegowda Moreira, Diogo R. M. LaLonde, Judith M. Bewley, Carole A. Abrams, Cameron F. Chaiken, Irwin M. TI A Model of Peptide Triazole Entry Inhibitor Binding to HIV-1 gp120 and the Mechanism of Bridging Sheet Disruption SO BIOCHEMISTRY LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; GENERAL FORCE-FIELD; ENVELOPE GLYCOPROTEIN; STRUCTURAL DETERMINANTS; MOLECULAR-DYNAMICS; RECEPTOR-BINDING; DUAL ANTAGONIST; CD4 RECEPTOR; ENV GP120; CONFORMATION AB Peptide triazole (PT) entry inhibitors prevent HIV-1 infection by blocking the binding of viral gp120 to both the HIV-1 receptor and the coreceptor on target cells. Here, we used all-atom explicit solvent molecular dynamics (MD) to propose a model for the encounter complex of the peptide triazoles with gp120. Saturation transfer difference nuclear magnetic resonance (STD NMR) and single-site mutagenesis experiments were performed to test the simulation results. We found that docking of the peptide to a conserved patch of residues lining the "F43 pocket" of gp120 in a bridging sheet naive gp120 conformation of the glycoprotein led to a stable complex. This pose prevents formation of the bridging sheet minidomain, which is required for receptor-coreceptor binding, providing a mechanistic basis for dual-site antagonism of this class of inhibitors. Burial of the peptide triazole at the gp120 inner domain outer domain-interface significantly contributed to complex stability and rationalizes the significant contribution of hydrophobic triazole groups to peptide potency. Both the simulation model and STD NMR experiments suggest that the I-X-W [where X is (2S,4S)-4-(4-phenyl-1H-1,2,3-triazol-1-yl)pyrrolidine] tripartite hydrophobic motif in the peptide is the major contributor of contacts at the gp120 PT interface. Because the model predicts that the peptide Trp side chain hydrogen bonding with gp120 S375 contributes to the stability of the PT gp120 complex, we tested this prediction through analysis of peptide binding to gp120 mutant S375A. The results showed that a peptide triazole KR21 inhibits S375A with 20-fold less potency than WT, consistent with predictions of the model. Overall, the PT gp120 model provides a starting point for both the rational design of higher-affinity peptide triazoles and the development of structure-minimized entry inhibitors that can trap gp120 into an inactive conformation and prevent infection. C1 [Emileh, Ali; Abrams, Cameron F.] Drexel Univ, Philadelphia, PA 19104 USA. [Emileh, Ali; Tuzer, Ferit; Umashankara, Muddegowda; Moreira, Diogo R. M.; Chaiken, Irwin M.] Drexel Univ, Coll Med, Dept Biochem & Mol Biol, Philadelphia, PA 19129 USA. [LaLonde, Judith M.] Bryn Mawr Coll, Dept Chem, Bryn Mawr, PA 19010 USA. [Yeh, Herman; Bewley, Carole A.] NIDDKD, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. RP Emileh, A (reprint author), Drexel Univ, Coll Med, Dept Biochem & Mol Biol, 11102 New Coll Bldg,245 N 15th St, Philadelphia, PA 19102 USA. EM ali.emileh@drexel.edu; cfa22@drexel.edu; ichaiken@drexelmed.edu OI Moreira, Diogo Rodrigo/0000-0003-3323-4404 FU National Institutes of Health (NIH) [5R21AI093248-02, 5R21AI091513-02, 5P01GM056550]; National Science Foundation (NSF) through TeraGrid/XSEDE allocation [MCB070073N]; CAPES-Fullbright scholarship; Intramural AIDS Targeted Antiviral Program; NIH Intramural Research Program (National Institute of Diabetes and Digestive and Kidney Diseases); DRACO GPU cluster in the Department of Physics at Drexel University (NSF) [AST-0959884] FX We acknowledge the National Institutes of Health (NIH) for support through Grants 5R21AI093248-02 and 5R21AI091513-02 (A.E., C.F.A., and I.M.C.) and Grant 5P01GM056550 (A.E., F.T., M.U, J.M.L., and I.M.C.) and the National Science Foundation (NSF) for computational support through TeraGrid/XSEDE allocation MCB070073N (C.F.A.). Funding for D.R.M.M. was provided through a CAPES-Fullbright scholarship. This work was supported in part by the Intramural AIDS Targeted Antiviral Program, Office of the Director, NIH (CAB.), and the NIH Intramural Research Program (National Institute of Diabetes and Digestive and Kidney Diseases). Part of the computational work was performed on the DRACO GPU cluster in the Department of Physics at Drexel University (NSF Grant AST-0959884). NR 67 TC 8 Z9 8 U1 2 U2 38 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD APR 2 PY 2013 VL 52 IS 13 BP 2245 EP 2261 DI 10.1021/bi400166b PG 17 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 120LX UT WOS:000317172900009 PM 23470147 ER PT J AU Gu, F Pfeiffer, RM Bhattacharjee, S Han, SS Taylor, PR Berndt, S Yang, H Sigurdson, AJ Toro, J Mirabello, L Greene, MH Freedman, ND Abnet, CC Dawsey, SM Hu, N Qiao, YL Ding, T Brenner, AV Garcia-Closas, M Hayes, R Brinton, LA Lissowska, J Wentzensen, N Kratz, C Moore, LE Ziegler, RG Chow, WH Savage, SA Burdette, L Yeager, M Chanock, SJ Chatterjee, N Tucker, MA Goldstein, AM Yang, XR AF Gu, F. Pfeiffer, R. M. Bhattacharjee, S. Han, S. S. Taylor, P. R. Berndt, S. Yang, H. Sigurdson, A. J. Toro, J. Mirabello, L. Greene, M. H. Freedman, N. D. Abnet, C. C. Dawsey, S. M. Hu, N. Qiao, Y-L Ding, T. Brenner, A. V. Garcia-Closas, M. Hayes, R. Brinton, L. A. Lissowska, J. Wentzensen, N. Kratz, C. Moore, L. E. Ziegler, R. G. Chow, W-H Savage, S. A. Burdette, L. Yeager, M. Chanock, S. J. Chatterjee, N. Tucker, M. A. Goldstein, A. M. Yang, X. R. TI Common genetic variants in the 9p21 region and their associations with multiple tumours SO BRITISH JOURNAL OF CANCER LA English DT Article DE common genetic variants; CDKN2A; 9p21.3 ID GENOME-WIDE ASSOCIATION; SINGLE-NUCLEOTIDE POLYMORPHISMS; SQUAMOUS-CELL CARCINOMA; IN-SITU HYBRIDIZATION; CHROMOSOME 9P21; SUSCEPTIBILITY LOCI; NONCODING RNA; BREAST-CANCER; MALIGNANT-MELANOMA; CANDIDATE GENES AB Background: The chromosome 9p21.3 region has been implicated in the pathogenesis of multiple cancers. Methods: We systematically examined up to 203 tagging SNPs of 22 genes on 9p21.3 (19.9-32.8 Mb) in eight case-control studies: thyroid cancer, endometrial cancer (EC), renal cell carcinoma, colorectal cancer (CRC), colorectal adenoma (CA), oesophageal squamous cell carcinoma (ESCC), gastric cardia adenocarcinoma and osteosarcoma (OS). We used logistic regression to perform single SNP analyses for each study separately, adjusting for study-specific covariates. We combined SNP results across studies by fixed-effect meta-analyses and a newly developed subset-based statistical approach (ASSET). Gene-based P-values were obtained by the minP method using the Adaptive Rank Truncated Product program. We adjusted for multiple comparisons by Bonferroni correction. Results: Rs3731239 in cyclin-dependent kinase inhibitors 2A (CDKN2A) was significantly associated with ESCC (P = 7 x 10(-6)). The CDKN2A-ESCC association was further supported by gene-based analyses (P-gene = 0.0001). In the meta-analyses by ASSET, four SNPs (rs3731239 in CDKN2A, rs615552 and rs573687 in CDKN2B and rs564398 in CDKN2BAS) showed significant associations with ESCC and EC (P < 2.46 x 10(-4)). One SNP in MTAP (methylthioadenosine phosphorylase) (rs7023329) that was previously associated with melanoma and nevi in multiple genome-wide association studies was associated with CRC, CA and OS by ASSET (P = 0.007). Conclusion: Our data indicate that genetic variants in CDKN2A, and possibly nearby genes, may be associated with ESCC and several other tumours, further highlighting the importance of 9p21.3 genetic variants in carcinogenesis. C1 [Gu, F.; Pfeiffer, R. M.; Bhattacharjee, S.; Han, S. S.; Taylor, P. R.; Berndt, S.; Yang, H.; Sigurdson, A. J.; Toro, J.; Mirabello, L.; Greene, M. H.; Freedman, N. D.; Abnet, C. C.; Dawsey, S. M.; Hu, N.; Brenner, A. V.; Garcia-Closas, M.; Hayes, R.; Brinton, L. A.; Wentzensen, N.; Kratz, C.; Moore, L. E.; Ziegler, R. G.; Chow, W-H; Savage, S. A.; Burdette, L.; Yeager, M.; Chanock, S. J.; Chatterjee, N.; Tucker, M. A.; Goldstein, A. M.; Yang, X. R.] NCI, Div Canc Epidemiol & Genet, Rockville, MD 20852 USA. [Qiao, Y-L] Chinese Acad Med Sci, Canc Inst & Hosp, Beijing 100021, Peoples R China. [Ding, T.] Shanxi Canc Hosp, Taiyuan 30013, Shanxi, Peoples R China. [Garcia-Closas, M.] Inst Canc Res, Div Genet & Epidemiol, Sutton SM2 5NG, Surrey, England. [Lissowska, J.] M Sklodowska Curie Canc Ctr & Inst Oncol, Dept Canc Epidemiol & Prevent, PL-02781 Warsaw, Poland. RP Yang, XR (reprint author), NCI, Div Canc Epidemiol & Genet, Rockville, MD 20852 USA. EM royang@mail.nih.gov RI Gu, Fangyi/I-5957-2014; Qiao, You-Lin/B-4139-2012; Tucker, Margaret/B-4297-2015; Abnet, Christian/C-4111-2015; Garcia-Closas, Montserrat /F-3871-2015; Brinton, Louise/G-7486-2015; Freedman, Neal/B-9741-2015; Savage, Sharon/B-9747-2015 OI Qiao, You-Lin/0000-0001-6380-0871; Lissowska, Jolanta/0000-0003-2695-5799; Abnet, Christian/0000-0002-3008-7843; Garcia-Closas, Montserrat /0000-0003-1033-2650; Brinton, Louise/0000-0003-3853-8562; Freedman, Neal/0000-0003-0074-1098; Savage, Sharon/0000-0001-6006-0740 FU NIH, NCI, DCEG FX We thank Xiaoqin Xiong for providing help with the data analyses. We thank iSelect principal investigators of ThC, EC, TGCT, RCC, CRC, CA, ESCC, GCA and OS studies for kindly providing data for this project. We thank Dr. Paula Hyland for her help in reviewing ENCODE data. This research was supported by the Intramural Research Program of the NIH, NCI, DCEG. NR 46 TC 14 Z9 15 U1 0 U2 25 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0007-0920 J9 BRIT J CANCER JI Br. J. Cancer PD APR 2 PY 2013 VL 108 IS 6 BP 1378 EP 1386 DI 10.1038/bjc.2013.7 PG 9 WC Oncology SC Oncology GA 118PX UT WOS:000317038800020 PM 23361049 ER PT J AU Ritchie, MD Denny, JC Zuvich, RL Crawford, DC Schildcrout, JS Bastarache, L Ramirez, AH Mosley, JD Pulley, JM Basford, MA Bradford, Y Rasmussen, LV Pathak, J Chute, CG Kullo, IJ McCarty, CA Chisholm, RL Kho, AN Carlson, CS Larson, EB Jarvik, GP Sotoodehnia, N Manolio, TA Li, RL Masys, DR Haines, JL Roden, DM AF Ritchie, Marylyn D. Denny, Joshua C. Zuvich, Rebecca L. Crawford, Dana C. Schildcrout, Jonathan S. Bastarache, Lisa Ramirez, Andrea H. Mosley, Jonathan D. Pulley, Jill M. Basford, Melissa A. Bradford, Yuki Rasmussen, Luke V. Pathak, Jyotishman Chute, Christopher G. Kullo, Iftikhar J. McCarty, Catherine A. Chisholm, Rex L. Kho, Abel N. Carlson, Christopher S. Larson, Eric B. Jarvik, Gail P. Sotoodehnia, Nona Manolio, Teri A. Li, Rongling Masys, Daniel R. Haines, Jonathan L. Roden, Dan M. CA Cohorts Heart Aging Res Genomic Ep TI Genome- and Phenome-Wide Analyses of Cardiac Conduction Identifies Markers of Arrhythmia Risk SO CIRCULATION LA English DT Article DE atrial fibrillation; electronic health records; genetics; genome-wide association study ID ELECTRONIC MEDICAL-RECORDS; SUPPRESSION TRIAL; MYOCARDIAL-INFARCTION; PERSONALIZED MEDICINE; RHEUMATOID-ARTHRITIS; COMMON VARIANTS; HEALTH RECORDS; QRS DURATION; DNA BIOBANK; PR INTERVAL AB Background-ECG QRS duration, a measure of cardiac intraventricular conduction, varies approximate to 2-fold in individuals without cardiac disease. Slow conduction may promote re-entrant arrhythmias. Methods and Results-We performed a genome-wide association study to identify genomic markers of QRS duration in 5272 individuals without cardiac disease selected from electronic medical record algorithms at 5 sites in the Electronic Medical Records and Genomics (eMERGE) network. The most significant loci were evaluated within the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium QRS genome-wide association study meta-analysis. Twenty-three single-nucleotide polymorphisms in 5 loci, previously described by CHARGE, were replicated in the eMERGE samples; 18 single-nucleotide polymorphisms were in the chromosome 3 SCN5A and SCN10A loci, where the most significant single-nucleotide polymorphisms were rs1805126 in SCN5A with P=1.2x10(-8) (eMERGE) and P=2.5x10(-20) (CHARGE) and rs6795970 in SCN10A with P=6x10(-6) (eMERGE) and P=5x10(-27) (CHARGE). The other loci were in NFIA, near CDKN1A, and near C6orf204. We then performed phenome-wide association studies on variants in these 5 loci in 13 859 European Americans to search for diagnoses associated with these markers. Phenome-wide association study identified atrial fibrillation and cardiac arrhythmias as the most common associated diagnoses with SCN10A and SCN5A variants. SCN10A variants were also associated with subsequent development of atrial fibrillation and arrhythmia in the original 5272 "heart-healthy" study population. Conclusions-We conclude that DNA biobanks coupled to electronic medical records not only provide a platform for genome-wide association study but also may allow broad interrogation of the longitudinal incidence of disease associated with genetic variants. The phenome-wide association study approach implicated sodium channel variants modulating QRS duration in subjects without cardiac disease as predictors of subsequent arrhythmias. (Circulation. 2013;127:1377-1385.) C1 [Ritchie, Marylyn D.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Denny, Joshua C.; Bastarache, Lisa] Vanderbilt Univ, Sch Med, Dept Biomed Informat, Nashville, TN 37232 USA. [Denny, Joshua C.; Ramirez, Andrea H.; Mosley, Jonathan D.; Roden, Dan M.] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA. [Zuvich, Rebecca L.; Crawford, Dana C.; Haines, Jonathan L.] Vanderbilt Univ, Sch Med, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA. [Roden, Dan M.] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37232 USA. [Crawford, Dana C.; Bradford, Yuki; Haines, Jonathan L.] Vanderbilt Univ, Sch Med, Ctr Human Genet Res, Nashville, TN 37232 USA. [Schildcrout, Jonathan S.] Vanderbilt Univ, Sch Med, Dept Biostat, Nashville, TN 37232 USA. [Pulley, Jill M.; Basford, Melissa A.] Vanderbilt Univ, Sch Med, Res Off, Nashville, TN 37232 USA. [McCarty, Catherine A.] Essentia Inst Rural Hlth, Duluth, MN USA. [Rasmussen, Luke V.; Kho, Abel N.] Northwestern Univ, Sch Med, Dept Prevent Med, Chicago, IL USA. [Chisholm, Rex L.] Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Chicago, IL USA. [Pathak, Jyotishman; Chute, Christopher G.] Mayo Clin, Div Biomed Informat & Stat, Rochester, MN USA. [Kullo, Iftikhar J.] Mayo Clin, Div Cardiol, Rochester, MN USA. [Carlson, Christopher S.] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Larson, Eric B.] Grp Hlth Res Inst, Seattle, WA USA. [Jarvik, Gail P.] Univ Washington, Dept Med, Div Med Genet, Seattle, WA 98195 USA. [Sotoodehnia, Nona] Univ Washington, Dept Med, Div Cardiol, Seattle, WA 98195 USA. [Sotoodehnia, Nona] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA 98195 USA. [Jarvik, Gail P.] Univ Washington, Dept Med, Div Genome Sci, Seattle, WA 98195 USA. [Masys, Daniel R.] Univ Washington, Dept Med, Div Biomed & Hlth Informat, Seattle, WA 98195 USA. [Manolio, Teri A.; Li, Rongling] NHGRI, NIH, Bethesda, MD 20892 USA. RP Roden, DM (reprint author), Vanderbilt Univ, Oates Inst Expt Therapeut, Sch Med, 1285 Med Res Bldg 4, Nashville, TN 37232 USA. EM dan.roden@vanderbilt.edu RI Jarvik, Gail/N-6476-2014; OI Jarvik, Gail/0000-0002-6710-8708; Rasmussen, Luke/0000-0002-4497-8049 FU Electronic Medical Records and Genomics (eMERGE) Network; National Human Genome Research Institute; National Institute of General Medical Sciences [U01-HG-004610, U01-HG-004608, U01-HG-04599, U01-HG-004609, U01-HG-04603]; National Center for Advancing Translational Sciences [UL1 RR024975, 2 UL1 TR000445]; National Library of Medicine [ROI-LM-010685]; [R01-HL088456] FX This work was supported by the Electronic Medical Records and Genomics (eMERGE) Network, initiated and funded by the National Human Genome Research Institute, with additional funding from the National Institute of General Medical Sciences, through the following grants: U01-HG-004610 (Group Health Cooperative), U01-HG-004608 (Marshfield Clinic), U01-HG-04599 (Mayo Clinic), U01-HG-004609 (Northwestern University), and U01-HG-04603 (Vanderbilt University, also serving as the Administrative Coordinating Center). BioVU also receives support through the National Center for Research Resources UL1 RR024975, which is now at the National Center for Advancing Translational Sciences, 2 UL1 TR000445. PheWAS method is also supported by ROI-LM-010685 from the National Library of Medicine. Dr Sotoodehnia was supported by R01-HL088456. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. NR 45 TC 69 Z9 71 U1 0 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD APR 2 PY 2013 VL 127 IS 13 BP 1377 EP + DI 10.1161/CIRCULATIONAHA.112.000604 PG 18 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 117JG UT WOS:000316948900013 PM 23463857 ER PT J AU Lardinois, O Hess, J Deterding, L Aybar, L Hogan, S Mcgregor, J Poulton, C Hu, Y Henderson, C Bunch, D Falk, R AF Lardinois, O. Hess, J. Deterding, L. Aybar, L. Hogan, S. Mcgregor, J. Poulton, C. Hu, Y. Henderson, C. Bunch, D. Falk, R. TI Abnormal glycosylation of serum IgG from patients with ANCA-associated systemic vasculitis: Relation to disease activity SO PRESSE MEDICALE LA English DT Meeting Abstract C1 [Lardinois, O.; Hess, J.; Aybar, L.; Hogan, S.; Mcgregor, J.; Poulton, C.; Hu, Y.; Henderson, C.; Bunch, D.; Falk, R.] UNC Kidney Ctr, Chapel Hill, NC USA. [Deterding, L.] NIEHS, Res Triangle Pk, NC USA. NR 3 TC 0 Z9 0 U1 0 U2 2 PU MASSON EDITEUR PI MOULINEAUX CEDEX 9 PA 21 STREET CAMILLE DESMOULINS, ISSY, 92789 MOULINEAUX CEDEX 9, FRANCE SN 0755-4982 EI 2213-0276 J9 PRESSE MED JI Presse Med. PD APR PY 2013 VL 42 IS 4 BP 735 EP 735 DI 10.1016/j.lpm.2013.02.198 PN 2 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA AA3FO UT WOS:000330978500251 ER PT J AU Miloslavsky, E Specks, U Merkel, P Seo, P Spiera, R Langford, C Hoffman, G Kallenberg, C St Clair, W Tchao, N Ding, L Ikle, D Jepson, B Brunetta, P Stone, J AF Miloslavsky, E. Specks, U. Merkel, P. Seo, P. Spiera, R. Langford, C. Hoffman, G. Kallenberg, C. St Clair, W. Tchao, N. Ding, L. Ikle, D. Jepson, B. Brunetta, P. Stone, J. TI Retreatment with Rituximab in the RAVE Trial SO PRESSE MEDICALE LA English DT Meeting Abstract ID MAINTENANCE C1 [Miloslavsky, E.; Stone, J.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Specks, U.] Mayo Clin, Rochester, MN USA. [Merkel, P.] Univ Penn, Philadelphia, PA 19104 USA. [Seo, P.] Johns Hopkins Univ, Baltimore, MD USA. [Spiera, R.] Hosp Special Surg, New York, NY 10021 USA. [Langford, C.; Hoffman, G.] Cleveland Clin Fdn, Cleveland, OH 44195 USA. [Kallenberg, C.] Univ Med Ctr Groningen, NL-9713 AV Groningen, Netherlands. [St Clair, W.] Duke Univ, Durham, NC 27706 USA. [Tchao, N.] Immune Tolerance Network, Bethesda, MD USA. [Ding, L.] NIAID, Bethesda, MD 20892 USA. [Ikle, D.; Jepson, B.] Rho, Chapel Hill, NC USA. [Brunetta, P.] Genentech Inc, San Francisco, CA 94080 USA. NR 3 TC 2 Z9 2 U1 0 U2 0 PU MASSON EDITEUR PI MOULINEAUX CEDEX 9 PA 21 STREET CAMILLE DESMOULINS, ISSY, 92789 MOULINEAUX CEDEX 9, FRANCE SN 0755-4982 EI 2213-0276 J9 PRESSE MED JI Presse Med. PD APR PY 2013 VL 42 IS 4 BP 778 EP 778 DI 10.1016/j.lpm.2013.02.300 PN 2 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA AA3FO UT WOS:000330978500352 ER PT J AU Miloslavsky, E Specks, U Merkel, P Seo, P Spiera, R Langford, C Hoffman, G Kallenberg, C St Clair, W Tchao, N Ding, L Ikle, D Jepson, B Brunetta, P Stone, J AF Miloslavsky, E. Specks, U. Merkel, P. Seo, P. Spiera, R. Langford, C. Hoffman, G. Kallenberg, C. St Clair, W. Tchao, N. Ding, L. Ikle, D. Jepson, B. Brunetta, P. Stone, J. TI Safety of remission induction with rituximab versus cyclophosphamide in patients 65 and older with severe ANCA-associated vasculitis SO PRESSE MEDICALE LA English DT Meeting Abstract ID POLYARTERITIS-NODOSA C1 [Miloslavsky, E.; Stone, J.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Specks, U.] Mayo Clin, Rochester, MN USA. [Merkel, P.] Univ Penn, Philadelphia, PA 19104 USA. [Seo, P.] Johns Hopkins Univ, Baltimore, MD USA. [Spiera, R.] Hosp Special Surg, New York, NY 10021 USA. [Langford, C.; Hoffman, G.] Cleveland Clin Fdn, Cleveland, OH 44195 USA. [Kallenberg, C.] Univ Med Ctr Groningen, NL-9713 AV Groningen, Netherlands. [St Clair, W.] Duke Univ, Durham, NC 27706 USA. [Tchao, N.] Immune Tolerance Network, Bethesda, MD USA. [Ding, L.] NIAID, Bethesda, MD 20892 USA. [Ikle, D.; Jepson, B.] Rho, Chapel Hill, NC USA. [Brunetta, P.] Genentech Inc, San Francisco, CA 94080 USA. NR 3 TC 1 Z9 1 U1 1 U2 4 PU MASSON EDITEUR PI MOULINEAUX CEDEX 9 PA 21 STREET CAMILLE DESMOULINS, ISSY, 92789 MOULINEAUX CEDEX 9, FRANCE SN 0755-4982 EI 2213-0276 J9 PRESSE MED JI Presse Med. PD APR PY 2013 VL 42 IS 4 BP 779 EP 780 DI 10.1016/j.lpm.2013.02.302 PN 2 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA AA3FO UT WOS:000330978500354 ER PT J AU Nelson, DE Jarman, DW Rehm, J Greenfield, TK Rey, G Kerr, WC Miller, P Shield, KD Ye, Y Naimi, TS AF Nelson, David E. Jarman, Dwayne W. Rehm, Juergen Greenfield, Thomas K. Rey, Gregoire Kerr, William C. Miller, Paige Shield, Kevin D. Ye, Yu Naimi, Timothy S. TI Alcohol-Attributable Cancer Deaths and Years of Potential Life Lost in the United States SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID CARDIOVASCULAR RISK-FACTORS; EPITHELIAL OVARIAN-CANCER; TELEPHONE SURVEY; BREAST-CANCER; METHODOLOGICAL ISSUES; ENDOMETRIAL CANCER; NONRESPONSE BIAS; PROSTATE-CANCER; POOLED ANALYSIS; RESPONSE RATES AB Objectives. Our goal was to provide current estimates of alcohol-attributable cancer mortality and years of potential life lost (YPLL) in the United States. Methods. We used 2 methods to calculate population-attributable fractions. We based relative risks on meta-analyses published since 2000, and adult alcohol consumption on data from the 2009 Alcohol Epidemiologic Data System, 2009 Behavioral Risk Factor Surveillance System, and 2009-2010 National Alcohol Survey. Results. Alcohol consumption resulted in an estimated 18 200 to 21 300 cancer deaths, or 3.2% to 3.7% of all US cancer deaths. The majority of alcohol-attributable female cancer deaths were from breast cancer (56% to 66%), whereas upper airway and esophageal cancer deaths were more common among men (53% to 71%). Alcohol-attributable cancers resulted in 17.0 to 19.1 YPLL for each death. Daily consumption of up to 20 grams of alcohol (<= 1.5 drinks) accounted for 26% to 35% of alcohol-attributable cancer deaths. Conclusions. Alcohol remains a major contributor to cancer mortality and YPLL. Higher consumption increases risk but there is no safe threshold for alcohol and cancer risk. Reducing alcohol consumption is an important and underemphasized cancer prevention strategy. C1 [Nelson, David E.; Miller, Paige] NCI, Bethesda, MD 20892 USA. [Jarman, Dwayne W.] US FDA, Detroit, MI USA. [Jarman, Dwayne W.] US PHS, Rockville, MD USA. [Rehm, Juergen; Shield, Kevin D.] Ctr Addict & Mental Hlth, Toronto, ON, Canada. [Greenfield, Thomas K.; Kerr, William C.; Ye, Yu] Inst Publ Hlth, Alcohol Res Grp, Emeryville, CA USA. [Rey, Gregoire] CepiDc, INSERM, Le Kremlin Bicetre, France. [Naimi, Timothy S.] Boston Univ, Med Ctr, Boston, MA USA. RP Nelson, DE (reprint author), 6120 Execut Blvd,Suite 150E, Bethesda, MD 20892 USA. EM nelsonde@mail.nih.gov OI /0000-0002-3108-4812 FU National Institute on Alcohol Abuse and Alcoholism(NIAAA Center) [P50 AA005595]; NIAAA [RO1-AA018377] FX Funding for the National Alcohol Survey and for T. K. Greenfield, W. C. Kerr, and Y. Ye was supported by the National Institute on Alcohol Abuse and Alcoholism(NIAAA Center grant P50 AA005595). T. S. Naimi's research was supported in part by NIAAA grant RO1-AA018377. NR 112 TC 62 Z9 62 U1 1 U2 17 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD APR PY 2013 VL 103 IS 4 BP 641 EP 648 DI 10.2105/AJPH.2012.301199 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AA0DU UT WOS:000330766100031 PM 23409916 ER PT J AU Or, C Collins, DR Merkur, AB Wang, YJ Chan, CC Forooghian, F AF Or, Chris Collins, David R. Merkur, Andrew B. Wang, Yujuan Chan, Chi-Chao Forooghian, Farzin TI Intravenous rituximab for the treatment of cancer-associated retinopathy SO CANADIAN JOURNAL OF OPHTHALMOLOGY-JOURNAL CANADIEN D OPHTALMOLOGIE LA English DT Letter ID AUTOIMMUNE RETINOPATHY; THERAPY; CELLS C1 [Or, Chris; Collins, David R.; Merkur, Andrew B.; Forooghian, Farzin] Univ British Columbia, Vancouver, BC V5Z 1M9, Canada. [Wang, Yujuan; Chan, Chi-Chao] NEI, NIH, Bethesda, MD 20892 USA. RP Forooghian, F (reprint author), Univ British Columbia, Vancouver, BC V5Z 1M9, Canada. EM farzin.forooghian@gmail.com RI wang, yujuan/C-8428-2016 NR 16 TC 5 Z9 6 U1 0 U2 2 PU CANADIAN OPHTHAL SOC PI OTTAWA PA 1525 CARLING AVE SUITE 610, OTTAWA, ONTARIO K1Z 8R9, CANADA SN 0008-4182 EI 1715-3360 J9 CAN J OPHTHALMOL JI Can. J. Opthalmol.-J. Can. Opthalmol. PD APR PY 2013 VL 48 IS 2 BP E35 EP E38 DI 10.1016/j.jcjo.2012.11.010 PG 5 WC Ophthalmology SC Ophthalmology GA AA5NX UT WOS:000331147800012 PM 23561621 ER PT J AU Osinusi, A AF Osinusi, Anu TI Treatment for HCV in HIV Coinfected subjects: A new era of hope? SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Osinusi, Anu] NIAID, CRMP, SAIC Frederick Inc, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 39 EP 39 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000018 ER PT J AU Arakelyan, A Fitzgerald, W Margolis, L Grivel, JC AF Arakelyan, Anush Fitzgerald, Wendy Margolis, Leonid Grivel, Jean-Charles TI Flow Virometry: A nanotechnology for analysis of individual viral particles SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Arakelyan, Anush; Fitzgerald, Wendy; Margolis, Leonid; Grivel, Jean-Charles] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 44 EP 44 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000028 ER PT J AU Arthos, J Cicala, C Van Ryk, D Wei, DL Jelicic, K Nawaz, F Hiatt, J Schwing, C Pascuccio, M Fauci, A AF Arthos, James Cicala, Claudia Van Ryk, Donald Wei, Danlan Jelicic, Katija Nawaz, Fatima Hiatt, Joseph Schwing, Catherine Pascuccio, Massimiliano Fauci, Anthony TI Glycosylation in HIV Transmission SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Arthos, James; Cicala, Claudia; Van Ryk, Donald; Wei, Danlan; Jelicic, Katija; Nawaz, Fatima; Hiatt, Joseph; Schwing, Catherine; Pascuccio, Massimiliano; Fauci, Anthony] NIAID, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 44 EP 44 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000029 ER PT J AU Subramaniam, S AF Subramaniam, Sriram TI Structure, dynamics and antigenicity in HIV Env: a bridge from structure to phenotype and function SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Subramaniam, Sriram] Natl Canc Inst, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 47 EP 47 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000034 ER PT J AU Pazgier, M Acharya, P Tolbert, WD Gohain, N Mengistu, M Wu, X Sajadi, MM Yu, L Liu, T Huang, W Luongo, TS Yang, Y Martin, L Ray, K Lacowicz, JR Robinson, J Kamin-Lewis, R Kwong, PD Guan, Y DeVico, AL Lewis, GK AF Pazgier, M. Acharya, P. Tolbert, W. D. Gohain, N. Mengistu, M. Wu, X. Sajadi, M. M. Yu, L. Liu, T. Huang, W. Luongo, T. S. Yang, Y. Martin, L. Ray, K. Lacowicz, J. R. Robinson, J. Kamin-Lewis, R. Kwong, P. D. Guan, Y. DeVico, A. L. Lewis, G. K. TI Structural definition of new transitional epitopes exposed by CD4 binding to HIV-1 SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Pazgier, M.; Tolbert, W. D.; Gohain, N.; Mengistu, M.; Wu, X.; Sajadi, M. M.; Yu, L.; Liu, T.; Huang, W.; Kamin-Lewis, R.; Guan, Y.; DeVico, A. L.; Lewis, G. K.] Univ Maryland, Sch Med, Inst Human Virol, Baltimore, MD USA. [Acharya, P.; Luongo, T. S.; Yang, Y.; Kwong, P. D.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD USA. [Martin, L.] CEA, iBiTecS, Serv Ingn Mol Prot, Gif Sur Yvette, France. [Ray, K.; Lacowicz, J. R.] Univ Maryland, Sch Med, Ctr Fluorescence Spect, Baltimore, MD USA. [Robinson, J.] Tulane Univ, Med Ctr, Dept Pediat, New Orleans, LA 70118 USA. RI Gohain, Neelakshi/P-7493-2015 NR 0 TC 0 Z9 0 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 48 EP 48 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000036 ER PT J AU Amin, MN McLellan, JS Huang, W Orwenyo, J Kwong, PD Wang, LX AF Amin, Mohammed N. McLellan, Jason S. Huang, Wei Orwenyo, Jared Kwong, Peter D. Wang, Lai-Xi TI Synthetic HIV-1 Glycopeptides Enable Characterization of Fine Epitopes of Broadly Neutralizing Antibody PG9 SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Amin, Mohammed N.; Huang, Wei; Orwenyo, Jared; Wang, Lai-Xi] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Inst Human Virol, Baltimore, MD 21201 USA. [McLellan, Jason S.; Kwong, Peter D.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 50 EP 50 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000040 ER PT J AU Zou, ZC Chastain, A Moir, S Cicala, C Crocker, P Arthos, J Sun, PD AF Zou, Zhongcheng Chastain, Ashley Moir, Susan Cicala, Claudia Crocker, Paul Arthos, James Sun, Peter D. TI The role of Siglec in HIV infection SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Zou, Zhongcheng; Chastain, Ashley; Moir, Susan; Cicala, Claudia; Crocker, Paul; Arthos, James; Sun, Peter D.] NIAID, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 50 EP 50 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000041 ER PT J AU Fauci, AS AF Fauci, Anthony S. TI Ending the HIV/AIDS Pandemic: Research and Implementation SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Fauci, Anthony S.] NIAID, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 55 EP 55 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000050 ER PT J AU Franchini, G AF Franchini, Genoveffa TI Antibodies to the gp120 envelope protein in protection of macaques from SIVmac251 acquisition SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Franchini, Genoveffa] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 55 EP 55 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000051 ER PT J AU Pavlakis, GN Jalah, R Valentin, A Rosati, M Patel, V Kulkarni, V Alicea, C Kelly, RB Guan, YJ Lifson, JD Hirsch, VM Venzon, D Broderick, KE Sardesai, NY LaBranche, C Montefiori, DC Shen, S Tomaras, G Felber, BK AF Pavlakis, George N. Jalah, Rashmi Valentin, Antonio Rosati, Margherita Patel, Vainav Kulkarni, Viraj Alicea, Candido Kelly, Rachel Beach Guan, Yongjun Lifson, Jeffrey D. Hirsch, Vanessa M. Venzon, David Broderick, Kate E. Sardesai, Niranjan Y. LaBranche, Celia Montefiori, David C. Shen, Shaunna Tomaras, Georgia Felber, Barbara K. TI Comparison of systemic and mucosal responses after DNA and protein co-immunization SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Jalah, Rashmi; Kulkarni, Viraj; Kelly, Rachel Beach; Felber, Barbara K.] NCI, Human Retrovirus Pathogenesis Sect, Vaccine Branch, CCR, Frederick, MD 21701 USA. [Pavlakis, George N.; Valentin, Antonio; Rosati, Margherita; Patel, Vainav; Alicea, Candido; Kelly, Rachel Beach] NCI, Human Retrovirus Sect, Vaccine Branch, CCR, Frederick, MD 21701 USA. [Guan, Yongjun] Inst Human Virol, Baltimore, MD USA. [Lifson, Jeffrey D.] NCI, AIDS & Canc Virus Program, SAIC, Frederick, MD 21701 USA. [Hirsch, Vanessa M.] NIAID, Viral Pathogenesis & Vaccine Sect, Bethesda, MD 20892 USA. [Venzon, David] NCI, Biostat & Data Management Sect, CCR, Bethesda, MD 20892 USA. [Broderick, Kate E.; Sardesai, Niranjan Y.] Inovio Pharmaceut Inc, Blue Bell, PA USA. [LaBranche, Celia; Montefiori, David C.; Shen, Shaunna; Tomaras, Georgia] Duke Univ, Med Ctr, Dept Surg, Lab AIDS Vaccine Res & Dev, Durham, NC 27710 USA. RI Tomaras, Georgia/J-5041-2016; bebarta, vikhyat/K-3476-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 56 EP 56 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000053 ER PT J AU Lowy, DR Day, PM Thompson, CD Pang, YS Kines, RC Schiller, JT AF Lowy, D. R. Day, P. M. Thompson, C. D. Pang, Y. S. Kines, R. C. Schiller, J. T. TI The mechanisms by which HPV vaccines induce protection against HPV-associated cancers SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Lowy, D. R.; Day, P. M.; Thompson, C. D.; Pang, Y. S.; Kines, R. C.; Schiller, J. T.] NCI, Cellular Oncol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 59 EP 59 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000058 ER PT J AU Farci, P Alter, HJ AF Farci, Patrizia Alter, Harvey J. TI Profibrogenic Chemokines and Viral Evolution Predict Rapid Progression of Hepatitis C to Cirrhosis SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Farci, Patrizia] NIAID, Hepat Pathogenesis Sect, Infect Dis Lab, Bethesda, MD USA. [Alter, Harvey J.] NIH, Dept Transfus Med, Ctr Clin, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 60 EP 60 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000060 ER PT J AU Acharya, P Luongo, T Louder, MK Louder, R Yang, YP Mckee, K Do Kwon, Y Kessler, P Martin, L Mascola, JR Kwong, PD AF Acharya, Priyamvada Luongo, Timothy Louder, Mark K. Louder, Robert Yang, Yongping Mckee, Krisha Do Kwon, Young Kessler, Pascal Martin, Loic Mascola, John R. Kwong, Peter D. TI Structural Basis for Broad and Potent HIV-1 neutralization by CD4-mimetic miniproteins SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Acharya, Priyamvada; Luongo, Timothy; Louder, Mark K.; Louder, Robert; Yang, Yongping; Mckee, Krisha; Do Kwon, Young; Kessler, Pascal; Martin, Loic; Mascola, John R.; Kwong, Peter D.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD USA. RI Louder, Robert/F-4713-2014 OI Louder, Robert/0000-0002-6944-9346 NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 64 EP 64 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000064 ER PT J AU Tosi, G Forlani, G Abdallah, R Andresen, V Turci, M Bertazzoni, U Franchini, G Poli, G Accolla, RS AF Tosi, Giovanna Forlani, Greta Abdallah, Rawan Andresen, Vibeke Turci, Marco Bertazzoni, Umberto Franchini, Genoveffa Poli, Guido Accolla, Roberto S. TI The MHC-II transactivator CIITA inhibits Tax-1-mediated HTLV-1 expression and NF-kBactivation SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Meeting Abstract CT 14th Annual International Meeting of the Institute-of-Human-Virology CY OCT 14-17, 2012 CL Univ Maryland Sch Med, Baltimore, MD SP Inst Human Virol HO Univ Maryland Sch Med C1 [Tosi, Giovanna; Forlani, Greta; Abdallah, Rawan; Accolla, Roberto S.] Univ Insubria, Dept Surg & Morphol Sci, I-21100 Varese, Italy. [Andresen, Vibeke] Univ Bergen, Inst Med, Hematol sect, N-5020 Bergen, Norway. [Turci, Marco; Bertazzoni, Umberto] Univ Verona, Sect Biol & Genet, Dept Life & Reprod Sci, I-37100 Verona, Italy. [Franchini, Genoveffa] NCI, Anim Models & Retroviral Vaccines Sect, Bethesda, MD 20892 USA. [Poli, Guido] Ist Sci San Raffaele, AIDS Immunopathogenesis Units, I-20132 Milan, Italy. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 EI 1077-9450 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR PY 2013 VL 62 SU 1 BP 71 EP 71 PG 1 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 300IQ UT WOS:000330458000077 ER PT J AU Moore, HM AF Moore, Helen M. TI Acquisition of Normal Tissues for the GTEx Program SO BIOPRESERVATION AND BIOBANKING LA English DT Editorial Material C1 [Moore, Helen M.] US Natl Canc Inst, Bethesda, MD USA. RP Moore, HM (reprint author), NCI, 11400 Rockville Pike,Suite 700, Bethesda, MD 20892 USA. EM moorehe@mail.nih.gov NR 1 TC 3 Z9 3 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1947-5535 EI 1947-5543 J9 BIOPRESERV BIOBANK JI Biopreserv. Biobank. PD APR PY 2013 VL 11 IS 2 BP 75 EP 76 DI 10.1089/bio.2013.1121 PG 2 WC Cell Biology; Chemistry, Applied; Medical Laboratory Technology SC Cell Biology; Chemistry; Medical Laboratory Technology GA 300GK UT WOS:000330452200001 PM 24845427 ER PT J AU Mucci, NR Moore, HM Brigham, LE Goldthwaite, CA Little, AR Lockhart, NC Scott, MP Struewing, JP Vincent, SL Compton, CC AF Mucci, Neil R. Moore, Helen M. Brigham, Lori E. Goldthwaite, Charles A. Little, A. Roger Lockhart, Nicole C. Scott, Michael P. Struewing, Jeffery P. Vincent, Stephen L. Compton, Carolyn C. CA Natl Canc Inst TI Meeting Research Needs with Postmortem Biospecimen Donation: Summary of Recommendations for Postmortem Recovery of Normal Human Biospecimens for Research SO BIOPRESERVATION AND BIOBANKING LA English DT Article ID HUMAN BRAIN; PROSTATE-CANCER; RNA INTEGRITY; MESSENGER-RNA; AUTOPSY; TISSUE; DEAD; OVERSIGHT; QUALITY; PH AB Normal human tissues, bodily fluids, and other biospecimens of known quality are essential for research to understand the development of cancer and other diseases and to develop new diagnostics and therapies. However, obtaining normal biospecimens appropriate for contemporary large-scale molecular and genomic research is one of the most challenging biospecimen acquisition problems for scientists and biospecimen resources that support research. Recognizing this challenge, the U.S. National Cancer Institute recently convened a series of workshops and meetings focused on the acquisition of normal tissues for research and produced an extensive document, Recommendations for Postmortem Recovery of Normal Human Biospecimens for Research. This article summarizes these recommendations, addressing key ethical, operational, and scientific elements for collecting normal reference biospecimens from postmortem donors in the U.S. Awareness of these recommendations can foster more effective collaborations and mitigate potential logistical challenges, while promoting postmortem biospecimen donation options for families and increasing the availability of high quality normal biospecimens for research. The recommendations have been put into practice in the collection of normal human biospecimens for the NIH Genotype-Tissue Expression Program (GTEx), a pilot study of human gene expression and regulation in multiple tissues which will provide valuable insights into the mechanisms of gene regulation and, in the future, its disease-related perturbations (http://commonfund.nih.gov/GTEx/). C1 [Mucci, Neil R.; Moore, Helen M.; Brigham, Lori E.; Goldthwaite, Charles A.; Little, A. Roger; Lockhart, Nicole C.; Scott, Michael P.; Struewing, Jeffery P.; Vincent, Stephen L.; Compton, Carolyn C.; Natl Canc Inst] NCI, Biorepositories & Biospecimen Res Branch, NIH, US Dept HHS, Bethesda, MD 20892 USA. RP Moore, HM (reprint author), NCI, 11400 Rockville Pike,Suite 700, Bethesda, MD 20892 USA. EM moorehe@mail.nih.gov RI Little, A/O-6191-2014; Struewing, Jeffery/I-7502-2013 OI Little, A/0000-0001-6831-0177; Struewing, Jeffery/0000-0002-4848-3334 NR 30 TC 5 Z9 5 U1 0 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1947-5535 EI 1947-5543 J9 BIOPRESERV BIOBANK JI Biopreserv. Biobank. PD APR PY 2013 VL 11 IS 2 BP 77 EP 82 DI 10.1089/bio.2012.0063 PG 6 WC Cell Biology; Chemistry, Applied; Medical Laboratory Technology SC Cell Biology; Chemistry; Medical Laboratory Technology GA 300GK UT WOS:000330452200002 PM 24845428 ER PT J AU Allen, AJ McNeely, JM Kisser, J Waldstein, SR Evans, MK Zonderman, AB AF Allen, Allyssa J. McNeely, Jessica M. Kisser, Jason Waldstein, Shari R. Evans, Michele K. Zonderman, Alan B. TI FOOD INSECURITY IS ASSOCIATED WITH GREATER HYPERTENSION PREVALENCE IN BLACKS AND WHITES SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Allen, Allyssa J.; Kisser, Jason; Waldstein, Shari R.] Univ Maryland Baltimore Cty, Baltimore, MD 21228 USA. [McNeely, Jessica M.] UMBC, Baltimore, MD USA. [Evans, Michele K.; Zonderman, Alan B.] NIA, Intramural Res Program, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A68 EP A68 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400218 ER PT J AU Arguelles, W Llabre, MM Penedo, FJ Talavera, GA Aviles-Santa, L Daviglus, ML Espinoza, RA Ostfeld, RJ Gonzalez, HM Rodriguez, CJ Schneiderman, N AF Arguelles, William Llabre, Maria M. Penedo, Frank J. Talavera, Gregory A. Aviles-Santa, Larissa Daviglus, Martha L. Espinoza, Rebeca A. Ostfeld, Robert J. Gonzalez, Hector M. Rodriguez, Carlos J. Schneiderman, Neil TI ACCULTURATION VERSUS CULTURAL RETENTION AMONG U. S. HISPANICS/LATINOS IN RELATION TO WAIST CIRCUMFERENCE: RESULTS FROM THE HISPANIC COMMUNITY HEALTH STUDY/STUDY OF LATINOS (HCHS/SOL) SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Arguelles, William] Univ Miami, Miami, FL USA. [Llabre, Maria M.; Schneiderman, Neil] Univ Miami, Coral Gables, FL 33124 USA. [Penedo, Frank J.; Daviglus, Martha L.] Northwestern Univ, Chicago, IL 60611 USA. [Talavera, Gregory A.] San Diego State Univ, San Diego, CA 92182 USA. [Aviles-Santa, Larissa] NHLBI, Bethesda, MD 20892 USA. [Espinoza, Rebeca A.] San Diego State Univ, Chula Vista, CA USA. [Ostfeld, Robert J.] Albert Einstein Coll Med, Bronx, NY 10467 USA. [Gonzalez, Hector M.] Wayne State Univ, Detroit, MI USA. [Rodriguez, Carlos J.] Wake Forest Univ, Winston Salem, NC 27109 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A93 EP A94 PG 2 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400297 ER PT J AU Kiecolt-Glaser, JK Antoni, MH Lutgendorf, SK Costanzo, ES Bower, JE McDonald, PA AF Kiecolt-Glaser, Janice K. Antoni, Michael H. Lutgendorf, Susan K. Costanzo, Erin S. Bower, Julienne E. McDonald, Paige A. TI PSYCHO-ONCOLOGY MEETS PSYCHONEUROIMMUNOLOGY AT THE CUTTING EDGE SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Kiecolt-Glaser, Janice K.] Ohio State Univ, Coll Med, Inst Behav Med Res, Columbus, OH 43210 USA. [Antoni, Michael H.] Univ Miami, Coral Gables, FL 33124 USA. [Lutgendorf, Susan K.] Univ Iowa, Iowa City, IA USA. [Costanzo, Erin S.] Univ Wisconsin, Psychiat & Carbone Canc Ctr, Madison, WI USA. [Bower, Julienne E.] Univ Calif Los Angeles, Los Angeles, CA USA. [McDonald, Paige A.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A17 EP A17 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400058 ER PT J AU McNeely, JM Waldstein, SR Evans, MK Zonderman, AB AF McNeely, Jessica M. Waldstein, Shari R. Evans, Michele K. Zonderman, Alan B. TI THE IMPACT OF POOR QUALITY RESIDENTIAL ENVIRONMENTS ON PHYSICAL AND MENTAL HEALTH RELATED QUALITY OF LIFE IN URBAN DWELLING ADULTS SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [McNeely, Jessica M.; Waldstein, Shari R.] UMBC, Baltimore, MD USA. [Evans, Michele K.] NIA, Immunol Lab, Baltimore, MD 21224 USA. [Zonderman, Alan B.] NIA, Lab Behav Epidemiol, Baltimore, MD 21224 USA. NR 0 TC 1 Z9 1 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A148 EP A148 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400470 ER PT J AU Murakami, H Katsunuma, R Kentaro, K Terasawa, Y Motomura, Y Kanayama, Y Mishima, K Moriguchi, Y Matsuda, H AF Murakami, Hiroki Katsunuma, Ruri Kentaro, Kentaro Terasawa, Yuri Motomura, Yuki Kanayama, Yusuke Mishima, Kazuo Moriguchi, Yoshiya Matsuda, Hiroshi TI NEURAL BASIS FOR AUTOGENIC TRAINING SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Murakami, Hiroki; Moriguchi, Yoshiya] Natl Ctr Neurol & Psychiat, Integrat Brain Imaging Ctr, Dept Clin Neuroimaging, Tokyo, Japan. [Katsunuma, Ruri; Kentaro, Kentaro; Terasawa, Yuri; Motomura, Yuki; Kanayama, Yusuke; Mishima, Kazuo] NIMH, Dept Psychophysiol, Bethesda, MD USA. [Matsuda, Hiroshi] Natl Ctr Neurol & Psychiat, Integrat Brain Imaging Ctr, Tokyo, Japan. NR 0 TC 0 Z9 0 U1 0 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A143 EP A143 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400455 ER PT J AU Nakajima, M Li, BS Kumar, S Ertin, E Scott, MS Wittmers, L al'Absi, M AF Nakajima, Motohiro Li, Bingshuo Kumar, Santosh Ertin, Emre Scott, Marcia S. Wittmers, Lorentz al'Absi, Mustafa TI VALIDATING A NOVEL WIRELESS AMBULATORY TECHNOLOGY IN THE ASSESSMENT OF STRESS: A PILOT STUDY SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Nakajima, Motohiro; Li, Bingshuo; Wittmers, Lorentz; al'Absi, Mustafa] Univ Minnesota, Sch Med, Duluth Med Res Inst, Duluth, MN 55812 USA. [Kumar, Santosh] Univ Memphis, Memphis, TN 38152 USA. [Ertin, Emre] Ohio State Univ, Columbus, OH 43210 USA. [Scott, Marcia S.] NIAAA, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A87 EP A88 PG 2 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400277 ER PT J AU Shah, MT Zonderman, AB Katzel, LI Waldstein, SR AF Shah, Mauli T. Zonderman, Alan B. Katzel, Leslie I. Waldstein, Shari R. TI DIFFERENTIAL SEX PATTERNS OF HABITUAL SLEEP DURATION AND CARDIOVASCULAR REACTIVITY IN HEALTHY OLDER ADULTS SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Shah, Mauli T.; Waldstein, Shari R.] Univ Maryland Baltimore Cty, Baltimore, MD 21228 USA. [Zonderman, Alan B.] NIA, Intramural Res Program, NIH, Baltimore, MD 21224 USA. [Katzel, Leslie I.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A156 EP A156 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400495 ER PT J AU Sprung, MR Zonderman, AB Evans, MK Waldstein, SR AF Sprung, Mollie R. Zonderman, Alan B. Evans, Michele K. Waldstein, Shari R. TI OBJECTIVE NEIGHBORHOOD CRIME IS DIFFERENTIALLY ASSOCIATED WITH CARDIOVASCULAR RISK FACTORS AS A FUNCTION OF RACE AND SEX SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Sprung, Mollie R.; Waldstein, Shari R.] Univ Maryland Baltimore Cty, Baltimore, MD 21228 USA. [Zonderman, Alan B.; Evans, Michele K.] NIA, Intramural Res Program, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A21 EP A21 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400072 ER PT J AU Thurlow, KL van Tilburg, MA Palsson, O Ramsden, C Ringel, Y Ringel-Kulka, T AF Thurlow, Katharine L. van Tilburg, Miranda A. Palsson, Olafur Ramsden, Christopher Ringel, Yehuda Ringel-Kulka, Tamar TI THE ROLE OF PSYCHOLOGICAL FACTORS IN THE CLINICAL MANIFESTATION OF EASTING ASSOCIATED SYMPTOMS SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract CT 71st Annual Scientific Meeting of the American-Psychosomatic-Society CY MAR 13-16, 2013 CL Miami, FL SP Amer Psychosomat Soc C1 [Thurlow, Katharine L.; van Tilburg, Miranda A.; Palsson, Olafur] Univ N Carolina, Dept Med, Div Gastroenterol & Hepatol, Chapel Hill, NC USA. [Ramsden, Christopher] NIAAA, Sect Nutr Neurosci, LMBB, NIH, Bethesda, MD USA. [Ringel, Yehuda] Univ N Carolina, UNC Gillings Sch Global Publ Hlth, Dept Med, Div Gastroenterol & Hepatol, Chapel Hill, NC USA. [Ringel-Kulka, Tamar] Univ N Carolina, UNC Gillings Sch Global Publ Hlth, Dept Maternal & Child Hlth, Chapel Hill, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 EI 1534-7796 J9 PSYCHOSOM MED JI Psychosom. Med. PD APR PY 2013 VL 75 IS 3 BP A112 EP A113 PG 2 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 300MG UT WOS:000330467400360 ER PT J AU Goodman, AB Blanck, HM Sherry, B Park, S Nebeling, L Yaroch, AL AF Goodman, Alyson B. Blanck, Heidi M. Sherry, Bettylou Park, Sohyun Nebeling, Linda Yaroch, Amy L. TI Behaviors and Attitudes Associated With Low Drinking Water Intake Among US Adults, Food Attitudes and Behaviors Survey, 2007 SO PREVENTING CHRONIC DISEASE LA English DT Article ID WEIGHT-LOSS; ENERGY-INTAKE; CONSUMPTION PATTERNS; CALORIC BEVERAGES; NATIONAL-HEALTH; BODY-WEIGHT; DIET; BMI; ADOLESCENTS; COMMUNITY AB Introduction Water is vital for life, and plain water is a calorie-free option for hydration. Increasing consumption of drinking water is a strategy to reduce energy intake and lose or maintain weight; however, information on the characteristics of consumers who drink water is limited. Our objective was to describe the characteristics of people who have a low intake of drinking water and to determine associations between their behaviors and attitudes and their intake of water. Methods We analyzed data from a nationally representative sample of 3,397 US adults who participated in the National Cancer Institute's 2007 Food Attitudes and Behaviors Survey. Multivariable logistic regression was used to identify sociodemographic characteristics and health-related behaviors and attitudes associated with self-reported drinking water intake of less than 4 cups per day. Results Overall, 7% of adults reported no daily consumption of drinking water, 36% reported drinking 1 to 3 cups, 35% reported drinking 4 to 7 cups, and 22% reported drinking 8 cups or more. The likelihood of drinking less than 4 cups of water daily was significantly higher among participants aged 55 years or older than among those aged 18 to 34 (adjusted odds ratio [AOR], 1.3), among residents of the Northeast than among residents of the South (AOR, 1.4), among participants who consumed 1 cup or less of fruits or vegetables per day than among those who consumed 4.5 cups or more (AOR, 3.0), among participants who did not exercise than among those who exercised 150 minutes or more per week (AOR, 1.7), and among participants who were neither trying to gain nor lose weight than among those trying to lose weight (AOR, 1.3). Conclusion Low drinking water intake was associated with age, region of residence, and several unhealthful behaviors and attitudes. Understanding characteristics associated with low drinking water intake may help to identify populations that could benefit from interventions to help adults drink more water. C1 [Goodman, Alyson B.] Ctr Dis Control & Prevent, Div Nutr Phys Act & Obes, Atlanta, GA 30341 USA. [Blanck, Heidi M.; Sherry, Bettylou; Park, Sohyun] Ctr Dis Control & Prevent, Atlanta, GA 30341 USA. [Nebeling, Linda] NCI, Washington, DC USA. [Yaroch, Amy L.] Swanson Ctr Nutr, Omaha, NE USA. RP Goodman, AB (reprint author), Ctr Dis Control & Prevent, Div Nutr Phys Act & Obes, 4770 Buford Highway NE,Mailstop K-26, Atlanta, GA 30341 USA. EM agoodman@cdc.gov NR 29 TC 3 Z9 3 U1 1 U2 8 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD APR PY 2013 VL 10 AR UNSP 120248 DI 10.5888/pcd10.120248 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 285JS UT WOS:000329390700006 ER PT J AU Wallace, RB Salive, ME AF Wallace, Robert B. Salive, Marcel E. TI The Dimensions of Multiple Chronic Conditions: Where Do We Go From Here? A Commentary on the Special Collection of Preventing Chronic Disease SO PREVENTING CHRONIC DISEASE LA English DT Editorial Material ID ELDERLY POPULATION; HEALTH OUTCOMES; OLDER PERSONS; PRIMARY-CARE; MULTIMORBIDITY; COMORBIDITY; GUIDELINES; PREVALENCE C1 [Wallace, Robert B.] Univ Iowa, Coll Publ Hlth, Iowa City, IA 52242 USA. [Salive, Marcel E.] NIA, Div Geriatr & Clin Gerontol, Bethesda, MD 20892 USA. RP Wallace, RB (reprint author), Univ Iowa, Coll Publ Hlth, 105 River St, Iowa City, IA 52242 USA. EM robert-wallace@uiowa.edu NR 31 TC 2 Z9 2 U1 0 U2 4 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD APR PY 2013 VL 10 AR UNSP 130104 DI 10.5888/pcd10.130104 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 285JS UT WOS:000329390700013 ER PT J AU Turo, D Otto, P Shah, JP Heimur, J Gebreab, T Zaazhoa, M Armstrong, K Gerber, LH Sikdar, S AF Turo, Diego Otto, Paul Shah, Jay P. Heimur, Juliana Gebreab, Tadesse Zaazhoa, Maryam Armstrong, Katherine Gerber, Lynn H. Sikdar, Siddhartha TI Ultrasonic Characterization of the Upper Trapezius Muscle in Patients with Chronic Neck Pain SO ULTRASONIC IMAGING LA English DT Article DE ultrasound; superficial muscle; texture image analysis; myofascial trigger point; tissue differentiation; tissue characterization; ultrasonic imaging; entropy filtering ID MYOFASCIAL TRIGGER POINTS; TEXTURE ANALYSIS; IMAGE TEXTURE; TISSUE AB Myofascial trigger points (MTrPs) are palpable, tender nodules in taut bands of skeletal muscle that are painful on compression. MTrPs are characteristic findings in myofascial pain syndrome (MPS). The role of MTrPs in the pathophysiology of MPS is unknown. Localization, diagnosis, and clinical outcome measures of painful MTrPs can be improved by objectively characterizing and quantitatively measuring their properties. The goal of this study was to evaluate whether ultrasound imaging and elastography can differentiate symptomatic (active) MTrPs from normal muscle. Patients with chronic (>3 months) neck pain with spontaneously painful, palpable (i.e., active) MTrPs and healthy volunteers without spontaneous pain (having palpably normal muscle tissue) were recruited for this study. The upper trapezius muscles in all subjects were imaged, and the echotexture was analyzed using entropy filtering of B-mode images. Vibration elastography was performed by vibrating the muscle externally at 100 Hz. Color Doppler variance imaging was used to quantify the regions of color deficit exhibiting low vibration amplitude. The imaging measures were compared against the clinical findings of a standardized physical exam. We found that sites with active MTrPs (n = 14) have significantly lower entropy (p < 0.05) and significantly larger nonvibrating regions (p < 0.05) during vibration elastography compared with normal, uninvolved muscle (n = 15). A combination of both entropy analysis and vibration elastography yielded 69% sensitivity and 81% specificity in discriminating active MTrPs from normal muscle. These results suggest that active MTrPs have more homogeneous texture and heterogeneous stiffness when compared with normal, unaffected muscle. Our methods enabled us to improve the imaging contrast between suspected MTrPs and surrounding muscle. Our results indicate that in subjects with chronic neck pain and active MTrPs, the abnormalities are not confined to discrete isolated nodules but instead affect the milieu of the muscle surrounding palpable MTrPs. With further refinement, ultrasound imaging can be a promising objective method for characterizing soft tissue abnormalities associated with active MTrPs and elucidating the role of MTrPs in the pathophysiology of MPS. C1 [Turo, Diego; Otto, Paul; Zaazhoa, Maryam; Armstrong, Katherine; Gerber, Lynn H.; Sikdar, Siddhartha] George Mason Univ, Fairfax, VA 22030 USA. [Shah, Jay P.; Heimur, Juliana; Gebreab, Tadesse] NIH, Bethesda, MD 20892 USA. RP Sikdar, S (reprint author), 4400 Univ Dr,MS 1G5, Fairfax, VA 22030 USA. EM ssikdar@gmu.edu FU National Institutes of Arthritis and Musculoskeletal and Skin Diseases at the National Institutes of Health [1R01-AR057348] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported in part by Grant 1R01-AR057348 from the National Institutes of Arthritis and Musculoskeletal and Skin Diseases at the National Institutes of Health. NR 19 TC 18 Z9 18 U1 1 U2 7 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0161-7346 EI 1096-0910 J9 ULTRASONIC IMAGING JI Ultrason. Imaging PD APR PY 2013 VL 35 IS 2 BP 173 EP 187 DI 10.1177/0161734612472408 PG 15 WC Acoustics; Engineering, Biomedical; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Engineering; Radiology, Nuclear Medicine & Medical Imaging GA 285LN UT WOS:000329395600008 PM 23493615 ER PT J AU Pepin, KM Marques-Toledo, C Scherer, L Morais, MM Ellis, B Eiras, AE AF Pepin, Kim M. Marques-Toledo, Cecilia Scherer, Luciano Morais, Maira M. Ellis, Brett Eiras, Alvaro E. TI Cost-effectiveness of Novel System of Mosquito Surveillance and Control, Brazil SO EMERGING INFECTIOUS DISEASES LA English DT Article ID RIO-DE-JANEIRO; AEDES-AEGYPTI DIPTERA; DENGUE-FEVER; DISEASE; MODEL; TRANSMISSION; REEMERGENCE; POPULATION; CULICIDAE; DENSITIES AB Of all countries in the Western Hemisphere, Brazil has the highest economic losses caused by dengue fever. We evaluated the cost-effectiveness of a novel system of vector surveillance and control, Monitoramento Inteligente da Dengue (Intelligent Dengue Monitoring System [MID]), which was implemented in 21 cities in Minas Gerais, Brazil. Traps for adult female mosquitoes were spaced at 300-m intervals throughout each city. In cities that used MID, vector control was conducted specifically at high-risk sites (indicated through daily updates by MID). In control cities, vector control proceeded according to guidelines of the Brazilian government. We estimated that MID prevented 27,191 cases of dengue fever and saved an average of $227 (median $58) per case prevented, which saved approximately $364,517 in direct costs (health care and vector control) and $7,138,940 in lost wages (societal effect) annually. MID was more effective in cities with stronger economies and more cost-effective in cities with higher levels of mosquito infestation. C1 [Pepin, Kim M.] NIH, Bethesda, MD 20892 USA. [Pepin, Kim M.] Colorado State Univ, Ft Collins, CO 80523 USA. [Marques-Toledo, Cecilia; Scherer, Luciano] Ecovec SA, Belo Horizonte, MG, Brazil. [Morais, Maira M.; Eiras, Alvaro E.] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil. [Ellis, Brett] Duke Natl Univ Singapore, Grad Med Sch, Singapore, Singapore. RP Eiras, AE (reprint author), Univ Fed Minas Gerais, Chem Ecol Insect Vector Lab, Belo Horizonte, MG, Brazil. EM alvaro@icb.ufmg.br FU Research and Policy for Infectious Disease Dynamics Program of the Science and Technology Directorate, US Department of Homeland Security; Fogarty International Center, National Institutes of Health; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (PRONEX-Dengue); Fundacao de Amparo a Pesquisa do Estado de Minas Gerais; Departamento de Ciencia e Tecnologia-Ministerio da Saude; Instituto Nacional de Ciencia e Tecnologia-Dengue FX K.M.P. was supported by the Research and Policy for Infectious Disease Dynamics Program of the Science and Technology Directorate, US Department of Homeland Security; and the Fogarty International Center, National Institutes of Health. A.E.E. was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (PRONEX-Dengue), Fundacao de Amparo a Pesquisa do Estado de Minas Gerais, Departamento de Ciencia e Tecnologia-Ministerio da Saude, and Instituto Nacional de Ciencia e Tecnologia-Dengue. NR 29 TC 19 Z9 20 U1 3 U2 13 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD APR PY 2013 VL 19 IS 4 BP 542 EP 550 DI 10.3201/eid1904.120117 PG 9 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 268LV UT WOS:000328173100003 PM 23628282 ER PT J AU Dickens, C Kew, MC Purcell, RH Kramvis, A AF Dickens, Caroline Kew, Michael C. Purcell, Robert H. Kramvis, Anna TI Occult Hepatitis B Virus Infection in Chacma Baboons, South Africa SO EMERGING INFECTIOUS DISEASES LA English DT Article ID MOLECULAR EPIDEMIOLOGY; NONHUMAN-PRIMATES; GENOTYPE-A; CHIMPANZEES; DNA; RELATEDNESS; SUBGENOTYPE; VARIANTS; SUBTYPE; DONORS AB During previous studies of susceptibility to hepatitis B virus (HBV) infection, HBV DNA was detected in 2/6 wild-caught baboons. In the present study, HBV DNA was amplified from 15/69 wild-caught baboons. All animals were negative for HBV surface antigen and antibody against HBV core antigen. Liver tissue from 1 baboon was immunohistochemically negative for HBV surface antigen but positive for HBV core antigen. The complete HBV genome of an isolate from this liver clustered with subgenotype A2. Reverse transcription PCR of liver RNA amplified virus precore and surface protein genes, indicating replication of virus in baboon liver tissue. Four experimentally naive baboons were injected with serum from HBV DNA positive baboons. These 4 baboons showed transient seroconversion, and HBV DNA was amplified from serum at various times after infection. The presence of HBV DNA at relatively low levels and in the absence of serologic markers in the baboon, a nonhuman primate, indicates an occult infection. C1 [Dickens, Caroline] Univ Witwatersrand, Dept Internal Med, ZA-2193 Johannesburg, South Africa. [Kew, Michael C.; Kramvis, Anna] Univ Witwatersrand, ZA-2193 Johannesburg, South Africa. [Kew, Michael C.] Groote Schuur Hosp, ZA-7925 Cape Town, South Africa. [Purcell, Robert H.] NIH, Bethesda, MD 20892 USA. RP Kramvis, A (reprint author), Univ Witwatersrand, Sch Med, Dept Internal Med, Hepatitis Virus Divers Res Programme, 7 York Rd, ZA-2193 Johannesburg, South Africa. EM anna.kramvis@wits.ac.za FU National Research Foundation, South Africa [NRF2K421]; Polio Research Foundation, South Africa [00/52]; National Research Foundation; Polio Research Foundation; Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health FX M.C.K. was supported by a grant (NRF2K421) from the National Research Foundation, South Africa. A.K. was supported by a grant (00/52) from the Polio Research Foundation, South Africa. C.D. was supported by bursaries from the National Research Foundation and the Polio Research Foundation. R.H.P. was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. NR 40 TC 5 Z9 5 U1 0 U2 2 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD APR PY 2013 VL 19 IS 4 BP 598 EP 605 DI 10.3201/eid1904.121107 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 268LV UT WOS:000328173100010 PM 23631817 ER PT J AU Silverman, M Cassab, R Muniz, R Shavitt, RG Toledo, MC Cappi, C Thayer, J de Mathis, A Diniz, J Hoexter, M Alcante, CD Borcato, S Hounie, AG Whitfield, J Belyavskaya, E Sternberg, E Miguel, E Marques, A AF Silverman, M. Cassab, R. Muniz, R. Shavitt, R. G. Toledo, M. C. Cappi, C. Thayer, J. de Mathis, A. Diniz, J. Hoexter, M. Alcante, C. D. Borcato, S. Hounie, A. G. Whitfield, J. Belyavskaya, E. Sternberg, E. Miguel, E. Marques, A. TI Does inflammation play a role in Obsessive Compulsive Disorder? SO JOURNAL OF OBSESSIVE-COMPULSIVE AND RELATED DISORDERS LA English DT Meeting Abstract C1 [Silverman, M.; Whitfield, J.; Belyavskaya, E.; Sternberg, E.; Miguel, E.; Marques, A.] NIMH, Sect Neuroendocrine Immunol & Behav, NIH, Bethesda, MD 20892 USA. [Cassab, R.; Muniz, R.; Shavitt, R. G.; Toledo, M. C.; Cappi, C.; de Mathis, A.; Diniz, J.; Hoexter, M.; Alcante, C. D.; Borcato, S.; Hounie, A. G.; Marques, A.] Univ Sao Paulo, Obsess Compuls Spectrum Disorders Program, Dept & Inst Psychiat, Sch Med, BR-05508 Sao Paulo, Brazil. [Thayer, J.] Ohio State Univ, Dept Psychol, Columbus, OH 43210 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-3649 J9 J OBSESS-COMPULS REL JI J. Obsessive-Compuls. Relat. Disord. PD APR PY 2013 VL 2 IS 2 BP 229 EP 229 PG 1 WC Psychiatry SC Psychiatry GA 257VB UT WOS:000327414800041 ER PT J AU McCoy, ES Lea, WA Mott, BT Maloney, DJ Jadhav, A Simeonov, A Zylka, MJ AF McCoy, Eric S. Lea, Wendy A. Mott, Bryan T. Maloney, David J. Jadhav, Ajit Simeonov, Anton Zylka, Mark J. TI High-Throughput Screen Identifies Cyclic Nucleotide Analogs That Inhibit Prostatic Acid Phosphatase SO JOURNAL OF BIOMOLECULAR SCREENING LA English DT Article DE ectonucleotidase; prostatic acid phosphatase; ACPP; pain; nociception ID ECTONUCLEOTIDASE; ADENOSINE AB The secretory and transmembrane isoforms of prostatic acid phosphatase (PAP) can dephosphorylate extracellular adenosine 5'-monophosphate (AMP) to adenosine, classifying PAP as an ectonucleotidase. Currently, there are no compounds that inhibit PAP in living cells. To identify small-molecule modulators of PAP, we used a 1536-well-based quantitative high-throughput fluorogenic assay to screen the Library of Pharmacologically Active Compounds (LOPAC(1280)) arrayed as eight-concentration dilution series. This fluorogenic assay used difluoro-4-methylumbelliferyl phosphate as substrate and collected data in kinetic mode. Candidate hits were subsequently tested in an orthogonal absorbance-based biochemical assay that used AMP as substrate. From these initial screens, three inhibitors of secretory human (h) and mouse (m) PAP were identified: 8-(4-chlorophenylthio) cAMP (pCPT-cAMP), calmidazolium chloride, and nalidixic acid. These compounds did not inhibit recombinant alkaline phosphatase. Of these compounds, only pCPT-cAMP and a related cyclic nucleotide analog (8-[4-chlorophenylthio] cGMP; pCPT-cGMP) inhibited the ectonucleotidase activity of transmembrane PAP in a cell-based assay. These cyclic nucleotides are structurally similar to AMP but cannot be hydrolyzed by PAP. In summary, we identified two cyclic nucleotide analogs that inhibit secretory and transmembrane PAP in vitro and in live cells. C1 [McCoy, Eric S.; Zylka, Mark J.] Univ N Carolina, Chapel Hill, NC 27599 USA. [Lea, Wendy A.; Mott, Bryan T.; Maloney, David J.; Jadhav, Ajit; Simeonov, Anton] NIH, Bethesda, MD 20892 USA. RP Zylka, MJ (reprint author), Univ N Carolina, Dept Cell & Mol Physiol, Ctr Neurosci, 5109D NRB,CB 7545,115 Mason Farm Rd, Chapel Hill, NC 27599 USA. EM asimeono@mail.nih.gov; zylka@med.unc.edu FU Molecular Libraries Initiative of the NIH Roadmap for Medical Research [U54MH084681]; Intramural Research Program of NHGRI, NIH; NINDS [R01NS067688] FX The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by a grant to A.S. from the Molecular Libraries Initiative of the NIH Roadmap for Medical Research (U54MH084681), the Intramural Research Program of NHGRI, NIH, and by a grant to M.J.Z. from NINDS (R01NS067688). NR 15 TC 1 Z9 1 U1 0 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1087-0571 EI 1552-454X J9 J BIOMOL SCREEN JI J. Biomol. Screen PD APR PY 2013 VL 18 IS 4 BP 481 EP 489 DI 10.1177/1087057112468613 PG 9 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry GA 136HC UT WOS:000318352000012 PM 23190738 ER PT J AU Alonso, A Krijthe, BP Aspelund, T Stepas, KA Pencina, MJ Moser, CB Sinner, MF Sotoodehnia, N Fontes, JD Janssens, ACJW Kronmal, RA Magnani, JW Witteman, JC Chamberlain, AM Lubitz, SA Schnabel, RB Agarwal, SK McManus, DD Ellinor, PT Larson, MG Burke, GL Launer, LJ Hofman, A Levy, D Gottdiener, JS Kaab, S Couper, D Harris, TB Soliman, EZ Stricker, BHC Gudnason, V Heckbert, SR Benjamin, EJ AF Alonso, Alvaro Krijthe, Bouwe P. Aspelund, Thor Stepas, Katherine A. Pencina, Michael J. Moser, Carlee B. Sinner, Moritz F. Sotoodehnia, Nona Fontes, Joao D. Janssens, A. Cecile J. W. Kronmal, Richard A. Magnani, Jared W. Witteman, Jacqueline C. Chamberlain, Alanna M. Lubitz, Steven A. Schnabel, Renate B. Agarwal, Sunil K. McManus, David D. Ellinor, Patrick T. Larson, Martin G. Burke, Gregory L. Launer, Lenore J. Hofman, Albert Levy, Daniel Gottdiener, John S. Kaeaeb, Stefan Couper, David Harris, Tamara B. Soliman, Elsayed Z. Stricker, Bruno H. C. Gudnason, Vilmundur Heckbert, Susan R. Benjamin, Emelia J. TI Simple Risk Model Predicts Incidence of Atrial Fibrillation in a Racially and Geographically Diverse Population: the CHARGE-AF Consortium SO JOURNAL OF THE AMERICAN HEART ASSOCIATION LA English DT Article DE atrial fibrillation; epidemiology; risk factors ID ATHEROSCLEROSIS RISK; AFRICAN-AMERICANS; SURVIVAL ANALYSIS; HEART-FAILURE; COMMUNITIES; FLUTTER; DESIGN; STROKE; PREVALENCE; WHITES AB Background-Tools for the prediction of atrial fibrillation (AF) may identify high-risk individuals more likely to benefit from preventive interventions and serve as a benchmark to test novel putative risk factors. Methods and Results-Individual-level data from 3 large cohorts in the United States (Atherosclerosis Risk in Communities [ARIC] study, the Cardiovascular Health Study [CHS], and the Framingham Heart Study [FHS]), including 18 556 men and women aged 46 to 94 years (19% African Americans, 81% whites) were pooled to derive predictive models for AF using clinical variables. Validation of the derived models was performed in 7672 participants from the Age, Gene and Environment-Reykjavik study (AGES) and the Rotterdam Study (RS). The analysis included 1186 incident AF cases in the derivation cohorts and 585 in the validation cohorts. A simple 5-year predictive model including the variables age, race, height, weight, systolic and diastolic blood pressure, current smoking, use of antihypertensive medication, diabetes, and history of myocardial infarction and heart failure had good discrimination (C-statistic, 0.765; 95% CI, 0.748 to 0.781). Addition of variables from the electrocardiogram did not improve the overall model discrimination (C-statistic, 0.767; 95% CI, 0.750 to 0.783; categorical net reclassification improvement, -0.0032; 95% CI, -0.0178 to 0.0113). In the validation cohorts, discrimination was acceptable (AGES C-statistic, 0.664; 95% CI, 0.632 to 0.697 and RS C-statistic, 0.705; 95% CI, 0.664 to 0.747) and calibration was adequate. Conclusion-A risk model including variables readily available in primary care settings adequately predicted AF in diverse populations from the United States and Europe. C1 [Alonso, Alvaro] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55454 USA. [Krijthe, Bouwe P.; Janssens, A. Cecile J. W.; Witteman, Jacqueline C.; Hofman, Albert; Stricker, Bruno H. C.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Stricker, Bruno H. C.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Stricker, Bruno H. C.] Erasmus MC, Dept Med Informat, Rotterdam, Netherlands. [Aspelund, Thor; Gudnason, Vilmundur] Iceland Heart Assoc, Res Inst, Kopavogur, Iceland. [Aspelund, Thor; Gudnason, Vilmundur] Univ Iceland, Reykjavik, Iceland. [Stepas, Katherine A.; Pencina, Michael J.; Moser, Carlee B.] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [Benjamin, Emelia J.] Boston Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA USA. [Lubitz, Steven A.; Ellinor, Patrick T.] Massachusetts Gen Hosp, Cardiac Arrhythmia Serv, Boston, MA 02114 USA. [Sotoodehnia, Nona] Univ Washington, Dept Med, Div Cardiol, Seattle, WA USA. [Sotoodehnia, Nona] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Kronmal, Richard A.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Heckbert, Susan R.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Sinner, Moritz F.; Fontes, Joao D.; Magnani, Jared W.; McManus, David D.; Larson, Martin G.; Levy, Daniel; Benjamin, Emelia J.] NHLBI, Framingham, MA USA. [Sinner, Moritz F.; Fontes, Joao D.; Magnani, Jared W.; McManus, David D.; Larson, Martin G.; Levy, Daniel; Benjamin, Emelia J.] Boston Univ, Framingham Heart Study, Framingham, MA USA. [Magnani, Jared W.; Benjamin, Emelia J.] Boston Univ, Sch Med, Dept Med, Boston, MA 02118 USA. [Chamberlain, Alanna M.] Mayo Clin, Dept Hlth Sci Res, Rochester, MN USA. [Sinner, Moritz F.; Lubitz, Steven A.; Ellinor, Patrick T.] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Charlestown, MA USA. [Schnabel, Renate B.] Univ Heart Ctr Hamburg Eppendorf, Dept Gen & Intervent Cardiol, Eppendorf, Germany. [Agarwal, Sunil K.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA. [Couper, David] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Biostat, Chapel Hill, NC USA. [Burke, Gregory L.] Wake Forest Sch Med, Div Publ Hlth Sci, Winston Salem, NC USA. [Launer, Lenore J.; Harris, Tamara B.] NIA, Lab Epidemiol Demog & Biometry, NIH, Bethesda, MD 20892 USA. [Levy, Daniel] NHLBI, Ctr Populat Studies, Bethesda, MD 20892 USA. [Gottdiener, John S.] Univ Maryland, Med Ctr, Div Cardiol, Baltimore, MD 21201 USA. [Sinner, Moritz F.; Kaeaeb, Stefan] Univ Munich, Univ Hosp Munich, Dept Med 1, Munich, Germany. [Soliman, Elsayed Z.] Wake Forest Univ, Sch Med, Dept Epidemiol & Prevent, Epidemiol Cardiol Res Ctr EPICARE, Winston Salem, NC 27109 USA. [Stricker, Bruno H. C.] Inspectorate Hlth Care, The Hague, Netherlands. [Kaeaeb, Stefan] Munich Heart Alliance, Munich, Germany. [McManus, David D.] Univ Massachusetts, Dept Med, Worcester, MA 01605 USA. [McManus, David D.] Univ Massachusetts, Dept Quantitat Hlth Sci, Worcester, MA 01605 USA. [McManus, David D.] Worcester Polytech Inst, Dept Biomed Engn, Worcester, MA 01609 USA. RP Alonso, A (reprint author), Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, 1300 S 2nd St,Suite 300, Minneapolis, MN 55454 USA. EM alonso@umn.edu; emelia@bu.edu RI Schnabel, Renate/F-6527-2014; Aspelund, Thor/C-5983-2008; Gudnason, Vilmundur/K-6885-2015; Alonso, Alvaro/A-4917-2010; OI Aspelund, Thor/0000-0002-7998-5433; Gudnason, Vilmundur/0000-0001-5696-0084; Alonso, Alvaro/0000-0002-2225-8323; Larson, Martin/0000-0002-9631-1254; Janssens, A Cecile/0000-0002-6153-4976; Benjamin, Emelia/0000-0003-4076-2336 FU NIH [N01-AG-12100]; NIA Intramural Research Program, Hjartavernd (the Icelandic Heart Association); Althingi (the Icelandic Parliament); National Heart, Lung, and Blood Institute [HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, HHSN268201100012C]; NHLBI [RC1HL099452, RC1HL101056, N01-HC-85239, N01-HC-85079, N01-HC-85086, N01-HC-35129, N01 HC-15103, N01 HC-55222, N01-HC-75150, N01-HC-45133, HL080295, R01HL088456]; American Heart Association [09SDG2280087, 09FTF2190028]; NIA [AG-023629, AG-15928, AG-20098, AG-027058]; German Heart Foundation; Erasmus Medical Center; Erasmus University, Rotterdam, Netherlands Organization for the Health Research and Development (ZonMw); Research Institute for Diseases in the Elderly (RIDE); Ministry of Education, Culture and Science; Ministry for Health, Welfare and Sports; European Commission (DG XII); Municipality of Rotterdam FX AGES: The Age, Gene/Environment Susceptibility Reykjavik Study has been funded by NIH contract N01-AG-12100, the NIA Intramural Research Program, Hjartavernd (the Icelandic Heart Association), and the Althingi (the Icelandic Parliament). ARIC: The Atherosclerosis Risk in Communities Study is carried out as a collaborative study supported by National Heart, Lung, and Blood Institute contracts (HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, and HHSN268201100012C). This work was additionally funded by grants RC1HL099452 and RC1HL101056 from NHLBI and 09SDG2280087 from the American Heart Association. CHS: The Cardiovascular Health Study was supported by NHLBI contracts N01-HC-85239, N01-HC-85079 through N01-HC-85086; N01-HC-35129, N01 HC-15103, N01 HC-55222, N01-HC-75150, N01-HC-45133 and NHLBI grants HL080295 and R01HL088456, with additional contribution from NINDS. Additional support was provided through AG-023629, AG-15928, AG-20098, and AG-027058 from the NIA. See also http://www.chs-nhlbi.org/pi.htm. FHS: 6R01-NS 17950, N01-HC 25195, 1R01HL092577 (to Drs. Ellinor and Benjamin), 1RC1HL101056 (to Drs. Alonso and Benjamin), 1R01HL102214 (to Drs. Heckbert and Benjamin), 1R01AG028321 (to Dr. Benjamin), American Heart Association award 09FTF2190028 (to Dr. Magnani) and 1R21HL106092 (to Dr. Magnani). Dr. Sinner was supported by the German Heart Foundation. RS: The Rotterdam Study is funded by Erasmus Medical Center and Erasmus University, Rotterdam, Netherlands Organization for the Health Research and Development (ZonMw), the Research Institute for Diseases in the Elderly (RIDE), the Ministry of Education, Culture and Science, the Ministry for Health, Welfare and Sports, the European Commission (DG XII), and the Municipality of Rotterdam. NR 40 TC 115 Z9 116 U1 2 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-9980 J9 J AM HEART ASSOC JI J. Am. Heart Assoc. PD APR PY 2013 VL 2 IS 2 AR UNSP e000102 DI 10.1161/JAHA.112.000102 PG 20 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 243ST UT WOS:000326338400002 PM 23537808 ER PT J AU Morris, AA Patel, RS Binongo, JNG Poole, J al Mheid, I Ahmed, Y Stoyanova, N Vaccarino, V Din-Dzietham, R Gibbons, GH Quyyumi, A AF Morris, Alanna A. Patel, Riyaz S. Binongo, Jose Nilo G. Poole, Joseph al Mheid, Ibhar Ahmed, Yusuf Stoyanova, Neli Vaccarino, Viola Din-Dzietham, Rebecca Gibbons, Gary H. Quyyumi, Arshed TI Racial Differences in Arterial Stiffness and Microcirculatory Function Between Black and White Americans SO JOURNAL OF THE AMERICAN HEART ASSOCIATION LA English DT Article DE arterial stiffness; racial disparities; reactive hyperemia ID CARDIOVASCULAR RISK-FACTORS; PULSE-WAVE VELOCITY; ALL-CAUSE MORTALITY; NITRIC-OXIDE; ENDOTHELIAL FUNCTION; AUGMENTATION INDEX; AFRICAN-AMERICANS; BLOOD-PRESSURE; REACTIVE HYPEREMIA; VASCULAR FUNCTION AB Background-Compared with whites, black Americans suffer from a disproportionate burden of cardiovascular disease (CVD). We hypothesized that racial differences in the prevalence of CVD could be attributed, in part, to impaired vascular function in blacks after adjustment for differences in risk factor burden. Methods and Results-We assessed vascular function in 385 black and 470 white subjects (mean age, 48 +/- 11 years; 45% male). Using digital pulse amplitude tonometry (EndoPAT) we estimated the reactive hyperemia index (RHI), a measure of microvascular endothelial function, and peripheral augmentation index (PAT-AIx). Central augmentation index (C-AIx) and pulse-wave velocity (PWV) were measured as indices of wave reflections and arterial stiffness, respectively, using applanation tonometry (Sphygmocor). Compared with whites, blacks had lower RHI (2.1 +/- 0.6 versus 2.3 +/- 0.6, P<0.001), greater arterial wave reflections assessed as both PAT-AIx (20.4 +/- 21.5 versus 17.0 +/- 22.4, P=0.01) and CAIx (20.8 +/- 12.3 versus 17.5 +/- 13.3, P=0.001), and greater arterial stiffness, measured as PWV (7.4 +/- 1.6 versus 7.1 +/- 1.6 m/s, P=0.001). After adjustment for traditional CVD risk factors, black race remained a significant predictor of lower RHI and higher PAT-AIx and CAIx (all P<0.001) in all subjects and of higher PWV in men (P=0.01). Furthermore, these associations persisted in a subgroup analysis of "healthy" individuals free of CVD risk factors. Conclusion-Black race is associated with impaired microvascular vasodilatory function, and greater large arterial wave reflections and stiffness. Because impairment in these vascular indices may be associated with worse long-term outcomes, they may represent underlying mechanisms for the increased CVD risk in blacks. C1 [Morris, Alanna A.; Patel, Riyaz S.; Poole, Joseph; al Mheid, Ibhar; Ahmed, Yusuf; Vaccarino, Viola; Quyyumi, Arshed] Emory Univ, Sch Med, Div Cardiol, Atlanta, GA 30322 USA. [Stoyanova, Neli; Din-Dzietham, Rebecca] Morehouse Sch Med, Cardiovasc Res Inst, Atlanta, GA 30310 USA. [Vaccarino, Viola] Emory Rollins Sch Publ Hlth, Div Epidemiol, Atlanta, GA USA. [Binongo, Jose Nilo G.] Emory Rollins Sch Publ Hlth, Dept Biostat & Bioinformat, Atlanta, GA USA. [Gibbons, Gary H.] NHLBI, NIH, Bethesda, MD 20892 USA. RP Morris, AA (reprint author), 1364 Clifton Rd,Suite D403B, Atlanta, GA 30322 USA. EM aamorr3@emory.edu FU NIH/NHLBI [1 U01HL079156-01, 1 U01HL79214-01]; NIH, National Center for Research Resources (NCRR) [M01-RR00039]; NIH/NCRR [5P20RR11104, 5U54RR022814]; NIH [K24HL077506-06]; Woodruff Fund (Emory Predictive Health Initiative) FX This work was supported by funding from NIH/NHLBI 1 U01 HL079156-01 (Quyyumi) and 1 U01HL79214-01 (Gibbons); NIH, National Center for Research Resources (NCRR) Grant M01-RR00039 for the Emory Clinical Interaction Unit (ACTSI) and NIH/NCRR 5P20RR11104 for the Morehouse CRC; NIH K24HL077506-06 (Vaccarino); NIH/NCRR 5U54RR022814 (Din); and the Woodruff Fund (Emory Predictive Health Initiative). NR 41 TC 22 Z9 22 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-9980 J9 J AM HEART ASSOC JI J. Am. Heart Assoc. PD APR PY 2013 VL 2 IS 2 AR UNSP e002154 DI 10.1161/JAHA.112.002154 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 243ST UT WOS:000326338400021 PM 23568343 ER PT J AU Arora, M Senadhi, V Arora, D Weinstock, J Dubin, E Okolo, PI Dutta, SK AF Arora, Manish Senadhi, Viplove Arora, Deepika Weinstock, Joyce Dubin, Ethan Okolo, Patrick I., III Dutta, Sudhir K. TI A critical evaluation and a search for the ideal colonoscopic preparation SO CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY LA English DT Article ID ORAL SODIUM-PHOSPHATE; BOWEL PREPARATION; LAVAGE SOLUTION; MAGNESIUM CITRATE; HYPERPHOSPHATEMIA; REGIMEN; IMPACT AB Objectives: The aim of this study was to evaluate the efficacy of various bowel preparations in accomplishing colonic cleansing for optimal mucosal visualization during colonoscopy. Methods: The study included a cohort of 980 patients who underwent colonoscopy at our endoscopy center within the last 3 years. All of the study patients were subdivided into four groups. Each group included 245 patients, all receiving a different type of bowel preparation. The bowel preparations used in this study included: magnesium citrate (Group I), a combination of oral sodium phosphate (fleets) and powder PEG-3350 (Group II), powder polyethylene glycol-3350 (PEG-3350 powder for Group III), and oral sodium phosphate (fleets for Group IV). A Colon Prep Score (CPS) was devised to compare the quality of the different bowel preparations used. The colonoscopy results from all of these patients were tabulated and analyzed statistically and expressed as mean +/- 1 standard deviation. Statistical analysis was performed using a one way ANOVA with Holm-Sidak method for intergroup analysis. Results: Group I patients received magnesium citrate and had a mean CPS +/- 1SD of 3.11 +/- 0.91. Group II patients (fleets and powder PEG-3350 combination) achieved a CPS of 3.37 +/- 1.16. The patients in Group III (powder PEG-3350) actually showed the highest mean CPS of 3.44 +/- 1.12. Group IV patients who used oral sodium phosphate alone reached a mean CPS of 3.23 +/- 1.01. Group III patients (powder PEG-3350 only) demonstrated a statistically higher CPS (P < 0.0006) in colon cleansing as compared to Group I patients (magnesium citrate). Similarly, Group II patients (oral sodium phosphate and powder PEG-3350 combination) also showed improved colon cleansing statistically (P < 0.006) as compared to Group I patients (magnesium citrate). Conclusions: Overall, all four colon preparations achieved an average CPS greater than 3.0 indicating clinically adequate colonic cleansing. However, powder PEG-3350 alone and in combination with oral sodium phosphate was observed to be statistically superior to magnesium citrate, when used for colon preparation for colonoscopy. (C) 2012 Elsevier Masson SAS. All rights reserved. C1 [Arora, Manish; Dutta, Sudhir K.] Univ Maryland, Sch Med, Div Gastroenterol, Baltimore, MD 21201 USA. [Arora, Manish] NIH, Baltimore, MD USA. [Senadhi, Viplove] Indiana Univ Sch Med, Div Gastroenterol & Hepatol, Indiana Inst Personalized Med, Indianapolis, IN 46202 USA. [Arora, Deepika] Elmhurst Hosp, Mt Sinai Sch Med, New York, NY USA. [Weinstock, Joyce; Dubin, Ethan; Dutta, Sudhir K.] Johns Hopkins Univ, Div Gastroenterol, Sinai Hosp Baltimore, Baltimore, MD USA. [Okolo, Patrick I., III] Johns Hopkins Univ Hosp, Div Gastroenterol, Baltimore, MD 21287 USA. RP Senadhi, V (reprint author), Indiana Univ, Div Gastroenterol & Hepatol, Regenstrief Suite 4100,1050 Wishard Blvd, Indianapolis, IN 46204 USA. EM vsenadhi@hotmail.com NR 26 TC 0 Z9 1 U1 0 U2 6 PU ELSEVIER MASSON PI MILANO PA VIA PALEOCAPA 7, 20121 MILANO, ITALY SN 2210-7401 EI 2210-741X J9 CLIN RES HEPATOL GAS JI Clin. Res. Hepatol. Gastroenterol. PD APR PY 2013 VL 37 IS 2 BP 200 EP 206 DI 10.1016/j.clinre.2012.05.015 PG 7 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 234CR UT WOS:000325618200024 PM 23084462 ER PT J AU Fiorentino, NM Lin, JS Ridder, KB Guttman, MA McVeigh, ER Blemker, SS AF Fiorentino, Niccolo M. Lin, Jonathan S. Ridder, Kathryn B. Guttman, Michael A. McVeigh, Elliot R. Blemker, Silvia S. TI Rectus Femoris Knee Muscle Moment Arms Measured in Vivo During Dynamic Motion With Real-Time Magnetic Resonance Imaging SO JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME LA English DT Article DE quadriceps; joint motion; magnetic resonance imaging; musculoskeletal geometry; real-time measurement ID PATELLAR TENDON; LEG MUSCLES; LENGTH; SOFTWARE; JOINT; MRI AB Moment arms represent a muscle's ability to generate a moment about a joint for a given muscle force. The goal of this study was to develop a method to measure muscle moment arms in vivo over a large range of motion using real-time magnetic resonance (MR) imaging. Rectus femoris muscle-tendon lengths and knee joint angles of healthy subjects (N = 4) were measured during dynamic knee joint flexion and extension in a large-bore magnetic resonance imaging (MRI) scanner. Muscle-tendon moment arms were determined at the knee using the tendon-excursion method by differentiating measured muscle-tendon length with respect to joint angle. Rectus femoris moment arms were averaged across a group of healthy subjects and were found to vary similarly during knee joint flexion (mean: 3.0 (SD 0.5) cm, maximum: 3.5 cm) and extension (mean: 2.8 (SD 0.4) cm, maximum: 3.6 cm). These moment arms compare favorably with previously published dynamic tendon-excursion measurements in cadaveric specimens but were relatively smaller than moment arms from center-of-rotation studies. The method presented here provides a new approach to measure muscle-tendon moment arms in vivo and has the potential to be a powerful resource for characterizing musculoskeletal geometry during dynamic joint motion. C1 [Fiorentino, Niccolo M.; Ridder, Kathryn B.; Blemker, Silvia S.] Univ Virginia, Dept Mech & Aerosp Engn, Charlottesville, VA 22908 USA. [Fiorentino, Niccolo M.; Lin, Jonathan S.; Guttman, Michael A.; McVeigh, Elliot R.] NHLBI, Cardiac Energet Lab, Div Intramural Res, NIH,DHHS, Bethesda, MD 20892 USA. [McVeigh, Elliot R.] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA. [Blemker, Silvia S.] Univ Virginia, Dept Biomed Engn, Charlottesville, VA 22908 USA. RP Blemker, SS (reprint author), Univ Virginia, Dept Mech & Aerosp Engn, Charlottesville, VA 22908 USA. EM ssblemker@virginia.edu RI Fiorentino, Niccolo/B-3951-2014; Blemker, Silvia/A-1661-2009 OI Fiorentino, Niccolo/0000-0003-0263-6769; FU National Heart, Lung, and Blood Institute in the National Institutes of Health [NIH R01 AR056201, NIH Z01 HL4004608]; National Science Foundation FX The authors would like to acknowledge the funding support of the National Heart, Lung, and Blood Institute in the National Institutes of Health, NIH R01 AR056201, NIH Z01 HL4004608 (E. McVeigh PI), and the National Science Foundation's Graduate Research Fellowship Program. NR 23 TC 2 Z9 2 U1 0 U2 7 PU ASME PI NEW YORK PA TWO PARK AVE, NEW YORK, NY 10016-5990 USA SN 0148-0731 EI 1528-8951 J9 J BIOMECH ENG-T ASME JI J. Biomech. Eng.-Trans. ASME PD APR PY 2013 VL 135 IS 4 AR 044501 DI 10.1115/1.4023523 PG 5 WC Biophysics; Engineering, Biomedical SC Biophysics; Engineering GA 240GF UT WOS:000326082300009 PM 24231903 ER PT J AU Jessup, CM Balbus, JM Christian, C Haque, E Howe, SE Newton, SA Reid, BC Roberts, L Wilhelm, E Rosenthal, JP AF Jessup, Christine M. Balbus, John M. Christian, Carole Haque, Ehsanul Howe, Sally E. Newton, Sheila A. Reid, Britt C. Roberts, Luci Wilhelm, Erin Rosenthal, Joshua P. TI Climate Change, Human Health, and Biomedical Research: Analysis of the National Institutes of Health Research Portfolio SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article DE climate change; climate variability; health impacts; health research; research portfolio ID INFECTIOUS-DISEASES; PUBLIC-HEALTH; SCIENCE AB BACKGROUND: According to a wide variety of analyses and projections, the potential effects of global climate change on human health are large and diverse. The U.S. National Institutes of Health (NIH), through its basic, clinical, and population research portfolio of grants, has been increasing efforts to understand how the complex interrelationships among humans, ecosystems, climate, climate variability, and climate change affect domestic and global health. OBJECTIVES: In this commentary we present a systematic review and categorization of the fiscal year (FY) 2008 NIH climate and health research portfolio. METHODS: A list of candidate climate and health projects funded from FY 2008 budget appropriations were identified and characterized based on their relevance to climate change and health and based on climate pathway, health impact, study type, and objective. RESULTS: This analysis identified seven FY 2008 projects focused on climate change, 85 climate-related projects, and 706 projects that focused on disease areas associated with climate change but did not study those associations. Of the nearly 53,000 awards that NIH made in 2008, approximately 0.17% focused on or were related to climate. CONCLUSIONS: Given the nature and scale of the potential effects of climate change on human health and the degree of uncertainty that we have about these effects, we think that it is helpful for the NIH to engage in open discussions with science and policy communities about government-wide needs and opportunities in climate and health, and about how NIH's strengths in human health research can contribute to understanding the health implications of global climate change. This internal review has been used to inform more recent initiatives by the NIH in climate and health. C1 [Jessup, Christine M.; Wilhelm, Erin; Rosenthal, Joshua P.] NIH, Fogarty Int Ctr, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Balbus, John M.; Newton, Sheila A.] NIEHS, NIH, Dept Hlth & Human Serv, Bethesda, MD USA. [Christian, Carole; Haque, Ehsanul] NIH, Div Program Coordinat Planning & Strateg Initiat, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Howe, Sally E.] NIH, Natl Lib Med, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Reid, Britt C.] NCI, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Roberts, Luci] NIH, Off Extramural Res, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Jessup, CM (reprint author), NIH, Div Int Training & Res, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM christine.jessup@nih.gov FU AAAS Science & Technology Policy Fellowship FX C.M.J. was supported by an AAAS Science & Technology Policy Fellowship. NR 32 TC 5 Z9 6 U1 4 U2 30 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP 399 EP 404 DI 10.1289/ehp.1104518 PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100020 PM 23552460 ER PT J AU Schug, TT Johnson, AF Balshaw, DM Garantziotis, S Walker, NJ Weis, C Nadadur, SS Birnbaum, LS AF Schug, Thaddeus T. Johnson, Anne F. Balshaw, David M. Garantziotis, Stavros Walker, Nigel J. Weis, Christopher Nadadur, Srikanth S. Birnbaum, Linda S. TI ONE Nano: NIEHS's Strategic Initiative on the Health and Safety Effects of Engineered Nanomaterials SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article DE consortium-based research; health effects; nanoparticles; nanotechnology ID POTENTIAL INHALATION EXPOSURE; CERIUM DIOXIDE NANOPARTICLES; ASSESSMENT TECHNIQUE NEAT; CARBON NANOTUBES; SENSOR ARRAY; QUANTUM DOTS; PART; IDENTIFICATION; MONOCYTES; SYSTEMS AB BACKGROUND: The past decade has seen tremendous expansion in the production and application of engineered nanomaterials (ENMs). The unique properties that make ENMs useful in the market-place also make their interactions with biological systems difficult to anticipate and critically important to explore. Currently, little is known about the health effects of human exposure to these materials. OBJECTIVES: As part of its role in supporting the National Nanotechnology Initiative, the National Institute of Environmental Health Sciences (NIEHS) has developed an integrated, strategic research program-"ONE Nano"-to increase our fundamental understanding of how ENMs interact with living systems, to develop predictive models for quantifying ENM exposure and assessing ENM health impacts, and to guide the design of second-generation ENMs to minimize adverse health effects. DISCUSSION: The NIEHS's research investments in ENM health and safety include extramural grants and grantee consortia, intramural research activities, and toxicological studies being conducted by the National Toxicology Program (NTP). These efforts have enhanced collaboration within the nanotechnology research community and produced toxicological profiles for selected ENMs, as well as improved methods and protocols for conducting in vitro and in vivo studies to assess ENM health effects. CONCLUSION: By drawing upon the strengths of the NIEHS's intramural, extramural, and NTP programs and establishing productive partnerships with other institutes and agencies across the federal government, the NIEHS's strategic ONE Nano program is working toward new advances to improve our understanding of the health impacts of engineered nanomaterials and support the goals of the National Nanotechnology Initiative. C1 [Schug, Thaddeus T.; Nadadur, Srikanth S.] NIEHS, Cellular Organs & Syst Pathobiol Branch, Div Extramural Res & Training, NIH,DHHS, Res Triangle Pk, NC 27709 USA. [Johnson, Anne F.] MDB Inc, Res Triangle Pk, NC USA. [Balshaw, David M.] NIEHS, Ctr Risk & Integrated Sci, Div Extramural Res & Training, NIH,DHHS, Res Triangle Pk, NC 27709 USA. [Garantziotis, Stavros] NIEHS, Climat Res Unit, NIH, DHHS, Res Triangle Pk, NC 27709 USA. [Walker, Nigel J.] NIEHS, Natl Toxicol Program, NIH, DHHS, Res Triangle Pk, NC 27709 USA. [Weis, Christopher; Birnbaum, Linda S.] NIEHS, Off Director, NIH, DHHS, Res Triangle Pk, NC 27709 USA. RP Schug, TT (reprint author), Div Extramural Res, 530 Davis Dr,Room 3041 Mail Drop K3-15, Morrisville, NC 27560 USA. EM schugt@niehs.nih.gov RI Walker, Nigel/D-6583-2012; Garantziotis, Stavros/A-6903-2009 OI Walker, Nigel/0000-0002-9111-6855; Garantziotis, Stavros/0000-0003-4007-375X FU NIEHS [HHSN273201100086U] FX A.F.J. is employed by MDB Inc. under a contract with the NIEHS (HHSN273201100086U). The authors declare they have no actual or potential competing financial interests. NR 41 TC 4 Z9 5 U1 1 U2 20 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP 410 EP 414 DI 10.1289/ehp.1206091 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100022 PM 23407114 ER PT J AU Winuthayanon, W Piyachaturawat, P Suksamrarn, A Burns, KA Arao, Y Hewitt, SC Pedersen, LC Korach, KS AF Winuthayanon, Wipawee Piyachaturawat, Pawinee Suksamrarn, Apichart Burns, Katherine A. Arao, Yukitomo Hewitt, Sylvia C. Pedersen, Lars C. Korach, Kenneth S. TI The Natural Estrogenic Compound Diarylheptanoid (D3): In Vitro Mechanisms of Action and in Vivo Uterine Responses via Estrogen Receptor alpha SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article DE diarylheptanoid; ER-dependent; nuclear translocation; phytoestrogen; uterus ID CURCUMA-COMOSA; MOUSE UTERUS; TRANSCRIPTIONAL ACTIVATION; BINDING DOMAIN; PHYTOESTROGENS; EXPRESSION; ESTRADIOL; GROWTH; GENE; RATS AB BACKGROUND: Diarylheptanoid (D3) isolated from the medicinal plant, Curcuma comosa, has estrogenic activity. OBJECTIVE: We aimed to elucidate the mechanism(s) of D3 action and compare it with that of 17 beta-estradiol (E-2) using both in vitro and in vivo uterine models. METHODS: We used human uterine (Ishikawa) cells to determine the estrogenic action of D3 on the activation and nuclear translocation of estrogen receptor alpha (ER alpha). In addition, we further characterized the uterine response to D3 treatment in vivo. RESULTS: D3 activated an estrogen responsive element (ERE) luciferase reporter through ER alpha, and molecular modeling suggested that D3 could be accommodated in the ER alpha binding pocket. Using modified ER alpha to assay ligand-dependent nuclear translocation, we observed D3-dependent ER alpha interaction and translocation. In mouse uteri, early-and late-phase estrogen-regulated gene responses were increased in D3-treated ovariectomized wild-type animals, in a manner similar to that of E-2; no response was seen in ER alpha knockout animals. We observed a divergence in estrogen responses after D3 treatment: D3 induced robust DNA synthesis in uterine epithelial cells, linked to an increase in cell-cycle-related genes; however, no increase in uterine weight was observed 24 hr after treatment. D3 also affected uterine progesterone receptor expression patterns similar to E-2. When D3 and E-2 were administered together, we observed no additive or antagonistic effects of D3 on E-2. Our findings suggest that D3 is a weak estrogenic agonist compound. CONCLUSION: D3 is a weakly acting phyto-estrogen that mimics the mitogenic responses produced by E-2 in an ER alpha-dependent manner, but it is unable to increase uterine weight or enhance or antagonize the effects of estrogen. C1 [Winuthayanon, Wipawee; Burns, Katherine A.; Arao, Yukitomo; Hewitt, Sylvia C.; Korach, Kenneth S.] NIEHS, Reprod & Dev Toxicol Lab, NIH, DHHS, Res Triangle Pk, NC 27709 USA. [Winuthayanon, Wipawee; Piyachaturawat, Pawinee] Mahidol Univ, Fac Sci, Dept Physiol, Bangkok 10400, Thailand. [Suksamrarn, Apichart] Ramkhamhang Univ, Fac Sci, Dept Chem, Bangkok, Thailand. [Pedersen, Lars C.] NIEHS, Struct Biol Lab, NIH, DHHS, Res Triangle Pk, NC 27709 USA. RP Korach, KS (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. EM korach@niehs.nih.gov OI Korach, Kenneth/0000-0002-7765-418X FU NIEHS Division of Intramural Research [Z01-ES70065, Z01-ES102645]; Thailand Research Fund through the Royal Golden Jubilee PhD program [PHD/0255/2546]; Target Research Grant [DBG5180020]; Strategic Basic Research Grant [DBG5280013] FX Research support was provided by the NIEHS Division of Intramural Research (Z01-ES70065 to K. S. K. and Z01-ES102645 to L. C. P), the Thailand Research Fund through the Royal Golden Jubilee PhD program (PHD/0255/2546 to P. P. and W. W.), a Target Research Grant (DBG5180020 to P. P.), and a Strategic Basic Research Grant (DBG5280013 to A.S.). NR 31 TC 5 Z9 5 U1 2 U2 13 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP 433 EP 439 DI 10.1289/ehp.1206122 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100026 PM 23552522 ER PT J AU Li, Y Luh, CJ Burns, KA Arao, Y Jiang, ZL Teng, CT Tice, RR Korach, KS AF Li, Yin Luh, Colin J. Burns, Katherine A. Arao, Yukitomo Jiang, Zhongliang Teng, Christina T. Tice, Raymond R. Korach, Kenneth S. TI Endocrine-Disrupting Chemicals (EDCs): In Vitro Mechanism of Estrogenic Activation and Differential Effects on ER Target Genes SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article DE E-2; EDCs; ER alpha; ER beta; ERE; ER target genes ID BISPHENOL-A; RECEPTOR-ALPHA; BETA; PATHWAY; BINDING; AGONIST; GROWTH; CELLS; ANTIESTROGENS; ANTAGONIST AB BACKGROUND: Endocrine-disrupting chemicals (EDCs) influence the activity of estrogen receptors (ERs) and alter the function of the endocrine system. However, the diversity of EDC effects and mechanisms of action are poorly understood. OBJECTIVES: We examined the agonistic activity of EDCs through ER alpha and ER beta. We also investigated the effects of EDCs on ER-mediated target genes. METHODS: HepG2 and HeLa cells were used to determine the agonistic activity of EDCs on ER alpha and ER beta via the luciferase reporter assay. Ishikawa cells stably expressing ER alpha were used to determine changes in endogenous ER target gene expression by EDCs. RESULTS: Twelve EDCs were categorized into three groups on the basis of product class and similarity of chemical structure. As shown by luciferase reporter analysis, the EDCs act as ER agonists in a cell type- and promoter-specific manner. Bisphenol A, bisphenol AF, and 2-2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (group 1) strongly activated ER alpha estrogen responsive element (ERE)-mediated responses. Daidzein, genistein, kaempferol, and coumestrol (group 2) activated both ER alpha and ER beta ERE-mediated activities. Endosulfan and kepone (group 3) weakly activated ER alpha. Only a few EDCs significantly activated the "tethered" mechanism via ER alpha or ER beta. Results of real-time polymerase chain reaction indicated that bisphenol A and bisphenol AF consistently activated endogenous ER target genes, but the activities of other EDCs on changes of ER target gene expression were compound specific. CONCLUSION: Although EDCs with similar chemical structures (in the same group) tended to have comparable ER alpha and ER beta ERE-mediated activities, similar chemical structure did not correlate with previously reported ligand binding affinities of the EDCs. Using ER alpha-stable cells, we observed that EDCs differentially induced activity of endogenous ER target genes. C1 [Li, Yin; Luh, Colin J.; Burns, Katherine A.; Arao, Yukitomo; Jiang, Zhongliang; Korach, Kenneth S.] NIEHS, Receptor Biol Sect, Reprod & Dev Toxicol Lab, Div Intramural Res,NIH,DHHS, Res Triangle Pk, NC 27709 USA. [Teng, Christina T.; Tice, Raymond R.] NIEHS, Biomol Screening Branch, Div Natl Toxicol Program, NIH,DHHS, Res Triangle Pk, NC 27709 USA. RP Korach, KS (reprint author), NIEHS, Reprod & Dev Toxicol Lab, POB 12233,MD B3-02, Res Triangle Pk, NC 27709 USA. EM korach@niehs.nih.gov OI Korach, Kenneth/0000-0002-7765-418X FU Division of Intramural Research of the National Institute of Environmental Health Sciences [Z01 ES70065]; National Institutes of Health FX Research support was provided by the Division of Intramural Research of the National Institute of Environmental Health Sciences to K. S. K. through Z01 ES70065, and from the National Institutes of Health summer internship program for C.J.L. NR 46 TC 26 Z9 29 U1 1 U2 44 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP 459 EP 466 DI 10.1289/ehp.1205951 PG 8 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100030 PM 23384675 ER PT J AU Schecter, A Lorber, M Guo, Y Wu, Q Yun, SH Kannan, K Hommel, M Imran, N Hynan, LS Cheng, DL Colacino, JA Birnbaum, LS AF Schecter, Arnold Lorber, Matthew Guo, Ying Wu, Qian Yun, Se Hun Kannan, Kurunthachalam Hommel, Madeline Imran, Nadia Hynan, Linda S. Cheng, Dunlei Colacino, Justin A. Birnbaum, Linda S. TI Phthalate Concentrations and Dietary Exposure from Food Purchased in New York State SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article DE BBzP; DEHP; DEP; DiBP; market basket survey; phthalate exposure ID US FOOD; ESTERS; SAMPLES; PLASTICIZERS; FOODSTUFFS; PRODUCTS; INFANTS; ADIPATE; MARKET AB BACKGROUND: Phthalates have been found in many personal care and industrial products, but have not previously been reported in food purchased in the United States. Phthalates are ubiquitous synthetic compounds and therefore difficult to measure in foods containing trace levels. Phthalates have been associated with endocrine disruption and developmental alteration. OBJECTIVES: Our goals were to report concentrations of phthalates in U. S. food for the first time, specifically, nine phthalates in 72 individual food samples purchased in Albany, New York, and to compare these findings with other countries and estimate dietary phthalate intake. METHODS: A convenience sample of commonly consumed foods was purchased from New York supermarkets. Methods were developed to analyze these foods using gas chromatography-mass spectroscopy. Dietary intakes of phthalates were estimated as the product of the food consumption rate and concentration of phthalates in that food. RESULTS: The range of detection frequency of individual phthalates varied from 6% for dicyclohexyl phthalate (DCHP) to 74% for di-2-ethylhexyl phthalate (DEHP). DEHP concentrations were the highest of the phthalates measured in all foods except beef [where di-n-octyl phthalate (DnOP) was the highest phthalate found], with pork having the highest estimated mean concentration of any food group (mean 300 ng/g; maximum, 1,158 ng/g). Estimated mean adult intakes ranged from 0.004 mu g/kg/day for dimethyl phthalate (DMP) to 0.673 mu g/kg/day for DEHP. CONCLUSIONS: Phthalates are widely present in U. S. foods. While estimated intakes for individual phthalates in this study were more than an order of magnitude lower than U. S. Environmental Protection Agency reference doses, cumulative exposure to phthalates is of concern and a more representa-tive survey of U. S. foods is indicated. C1 [Schecter, Arnold; Hommel, Madeline; Imran, Nadia; Cheng, Dunlei] Univ Texas Sch Publ Hlth, Dallas, TX 75390 USA. [Lorber, Matthew] US EPA, Natl Ctr Environm Assessment, Off Res & Dev, Washington, DC 20460 USA. [Guo, Ying; Wu, Qian; Yun, Se Hun; Kannan, Kurunthachalam] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA. [Wu, Qian; Yun, Se Hun; Kannan, Kurunthachalam] SUNY Albany, Dept Environm Hlth Sci, Albany, NY 12222 USA. [Hynan, Linda S.] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA. [Colacino, Justin A.] Univ Michigan, Ann Arbor, MI 48109 USA. [Birnbaum, Linda S.] NCI, NIH, US Dept HHS, Res Triangle Pk, NC USA. [Birnbaum, Linda S.] NIEHS, NIH, US Dept HHS, Res Triangle Pk, NC 27709 USA. RP Schecter, A (reprint author), Univ Texas Sch Publ Hlth, 6011 Harry Hines Blvd,V8-122E, Dallas, TX 75390 USA. EM arnold.schecter@utsouthwestern.edu OI Hynan, Linda/0000-0002-4642-7769 FU Gustavus and Louise Pfeiffer Research Foundation; National Institutes of Health (NIH), National Cancer Institute (NCI); National Institute of Environmental Health Sciences (NIEHS), NIH [T32 ES007062]; National Human Genome Research Institute [T32 HG00040] FX This study was funded by the Gustavus and Louise Pfeiffer Research Foundation and and funded in part by the intramural research program of the National Institutes of Health (NIH), National Cancer Institute (NCI). Support for J.A.C. was provided by training grants from the National Institute of Environmental Health Sciences (NIEHS), NIH (T32 ES007062), and the National Human Genome Research Institute (T32 HG00040). NR 34 TC 71 Z9 76 U1 13 U2 87 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP 473 EP 479 DI 10.1289/ehp.1206367 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100032 PM 23461894 ER PT J AU Bergman, A Heindel, JJ Kasten, T Kidd, KA Jobling, S Neira, M Zoeller, RT Becher, G Bjerregaard, P Bornman, R Brandt, I Kortenkamp, A Muir, D Drisse, MNB Ochieng, R Skakkebaek, NE Bylehn, AS Iguchi, T Toppari, J Woodruff, TJ AF Bergman, Ake Heindel, Jerrold J. Kasten, Tim Kidd, Karen A. Jobling, Susan Neira, Maria Zoeller, R. Thomas Becher, Georg Bjerregaard, Poul Bornman, Riana Brandt, Ingvar Kortenkamp, Andreas Muir, Derek Drisse, Marie-Noel Brune Ochieng, Roseline Skakkebaek, Niels E. Bylehn, Agneta Sunden Iguchi, Taisen Toppari, Jorma Woodruff, Tracey J. TI The Impact of Endocrine Disruption: A Consensus Statement on the State of the Science SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Editorial Material C1 [Bergman, Ake] Stockholm Univ, Dept Mat & Environm Chem, SE-10691 Stockholm, Sweden. [Heindel, Jerrold J.] NIEHS, NIH, US Dept HHS, Res Triangle Pk, NC 27709 USA. [Kasten, Tim] UNEP, Geneva, Switzerland. [Kidd, Karen A.] Univ New Brunswick, Dept Biol, St John, NB E2L 4L5, Canada. [Kidd, Karen A.] Univ New Brunswick, Canadian Rivers Inst, St John, NB E2L 4L5, Canada. [Jobling, Susan; Kortenkamp, Andreas] Brunel Univ, Inst Environm, Uxbridge UB8 3PH, Middx, England. [Neira, Maria; Drisse, Marie-Noel Brune] WHO, Dept Publ Hlth & Environm, CH-1211 Geneva, Switzerland. [Zoeller, R. Thomas] Univ Massachusetts, Morrill Sci Ctr, Dept Biol, Amherst, MA 01003 USA. [Becher, Georg] Norwegian Inst Publ Hlth, Div Environm Med, Oslo, Norway. [Bjerregaard, Poul] Univ Southern Denmark, Inst Biol, Odense, Denmark. [Bornman, Riana] Univ Pretoria, Dept Urol, Steve Biko Acad Hosp, Sch Med, Pretoria, South Africa. [Brandt, Ingvar] Uppsala Univ, Dept Environm Toxicol, Uppsala, Sweden. [Muir, Derek] Environm Canada, Water Sci & Technol Directorate, Aquat Ecosyst Protect Res Div, Burlington, ON L7R 4A6, Canada. [Ochieng, Roseline] Aga Khan Univ Hosp, Dept Paediat & Child Hlth, Nairobi, Kenya. [Skakkebaek, Niels E.] Rigshosp, Univ Dept Growth & Reprod, DK-2100 Copenhagen, Denmark. [Iguchi, Taisen] Natl Inst Nat Sci, Natl Inst Basic Biol, Okazaki Inst Integrat Biosci, Dept Environm Sci, Okazaki, Aichi 4448585, Japan. [Toppari, Jorma] Univ Turku, Dept Physiol, Turku, Finland. [Toppari, Jorma] Univ Turku, Dept Paediat, Turku, Finland. [Woodruff, Tracey J.] Univ Calif San Francisco, Inst Hlth Policy Studies, Dept Obstet Gynecol & Reprod Sci, Oakland, CA USA. RP Bergman, A (reprint author), Stockholm Univ, Dept Mat & Environm Chem, SE-10691 Stockholm, Sweden. EM ake.bergman@mmk.su.se RI Bjerregaard, Poul/G-3621-2013 OI Brandt, Ingvar/0000-0002-5386-2400; Muir, Derek/0000-0001-6631-9776; Bergman, Ake/0000-0003-3403-093X; Kidd, Karen/0000-0002-5619-1358; Bjerregaard, Poul/0000-0002-2065-7232 NR 0 TC 60 Z9 62 U1 6 U2 62 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP A104 EP A106 DI 10.1289/ehp.1205448 PG 3 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100001 PM 23548368 ER PT J AU Birnbaum, LS Thayer, KA Bucher, JR Wolfe, MS AF Birnbaum, Linda S. Thayer, Kristina A. Bucher, John R. Wolfe, Mary S. TI Implementing Systematic Review at the National Toxicology Program: Status and Next Steps SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Editorial Material C1 [Birnbaum, Linda S.; Thayer, Kristina A.; Bucher, John R.; Wolfe, Mary S.] NIEHS, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA. RP Birnbaum, LS (reprint author), NIEHS, NIH, Dept Hlth & Human Serv, POB 12233, Res Triangle Pk, NC 27709 USA. EM bucher@niehs.nih.gov NR 12 TC 16 Z9 16 U1 0 U2 0 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP A108 EP A109 DI 10.1289/ehp.1306711 PG 2 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100003 PM 23548834 ER PT J AU Birnbaum, LS AF Birnbaum, Linda S. TI State of the Science of Endocrine Disruptors SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Editorial Material C1 [Birnbaum, Linda S.] NIEHS, Res Triangle Pk, NC 27709 USA. [Birnbaum, Linda S.] NIH, NTP, Dept Hlth & Human Serv, Res Triangle Pk, NC USA. RP Birnbaum, LS (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. EM birnbaumls@niehs.nih.gov NR 6 TC 15 Z9 15 U1 0 U2 10 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD APR PY 2013 VL 121 IS 4 BP A107 EP A107 DI 10.1289/ehp.1306695 PG 1 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA 208UM UT WOS:000323706100002 PM 23548815 ER PT J AU Shankavaram, U Fliedner, SMJ Elkahloun, AG Barb, JJ Munson, PJ Huynh, TT Matro, JC Turkova, H Linehan, WM Timmers, HJ Tischler, AS Powers, JF de Krijger, R Baysal, BE Takacova, M Pastorekova, S Gius, D Lehnert, H Camphausen, K Pacak, K AF Shankavaram, Uma Fliedner, Stephanie M. J. Elkahloun, Abdel G. Barb, Jenifer J. Munson, Peter J. Huynh, Thanh T. Matro, Joey C. Turkova, Hana Linehan, W. Marston Timmers, Henri J. Tischler, Arthur S. Powers, James F. de Krijger, Ronald Baysal, Bora E. Takacova, Martina Pastorekova, Silvia Gius, David Lehnert, Hendrik Camphausen, Kevin Pacak, Karel TI Genotype and Tumor Locus Determine Expression Profile of Pseudohypoxic Pheochromocytomas and Paragangliomas SO NEOPLASIA LA English DT Article ID HIPPEL-LINDAU-SYNDROME; CELL RENAL-CARCINOMA; FACTOR-KAPPA-B; CLINICAL CHARACTERISTICS; SDH MUTATIONS; GENE; SUPPRESSOR; CANCER; HEREDITARY; SURVIVAL AB Pheochromocytomas (PHEOs) and paragangliomas (PGLs) related to mutations in the mitochondrial succinate dehydrogenase (SDH) subunits A, B, C, and D, SDH complex assembly factor 2, and the von Hippel-Lindau (VHL) genes share a pseudohypoxic expression profile. However, genotype-specific differences in expression have been emerging. Development of effective new therapies for distinctive manifestations, e. g., a high rate of malignancy in SDHB- or predisposition to multifocal PGLs in SDHD patients, mandates improved stratification. To identify mutation/location-related characteristics among pseudohypoxic PHEOs/PGLs, we used comprehensive microarray profiling (SDHB: n = 18, SDHD-abdominal/thoracic (AT): n = 6, SDHD-head/neck (HN): n = 8, VHL: n = 13). To avoid location-specific bias, typical adrenal medulla genes were derived from matched normal medullas and cortices (n = 8) for data normalization. Unsupervised analysis identified two dominant clusters, separating SDHB and SDHD-AT PHEOs/PGLs (cluster A) from VHL PHEOs and SDHD-HN PGLs (cluster B). Supervised analysis yielded 6937 highly predictive genes (misclassification error rate of 0.175). Enrichment analysis revealed that energy metabolism and inflammation/fibrosis-related genes were most pronouncedly changed in clusters A and B, respectively. A minimum subset of 40 classifiers was validated by quantitative real-time polymerase chain reaction (quantitative real-time polymerase chain reaction vs. microarray: r = 0.87). Expression of several individual classifiers was identified as characteristic for VHL and SDHD-HN PHEOs and PGLs. In the present study, we show for the first time that SDHD-HN PGLs share more features with VHL PHEOs than with SDHD-AT PGLs. The presented data suggest novel subclassification of pseudohypoxic PHEOs/PGLs and implies cluster-specific pathogenic mechanisms and treatment strategies. C1 [Shankavaram, Uma; Gius, David; Camphausen, Kevin] NCI, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA. [Fliedner, Stephanie M. J.; Huynh, Thanh T.; Matro, Joey C.; Turkova, Hana; Pacak, Karel] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Med Neuroendocrinol, Program Reprod & Adult Endocrinol, NIH, Bethesda, MD 20892 USA. [Shankavaram, Uma; Gius, David; Camphausen, Kevin] Univ Hosp Schleswig Holstein, Dept Med 1, Lubeck, Germany. [Elkahloun, Abdel G.] NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. [Barb, Jenifer J.; Munson, Peter J.] NIH, Math & Stat Comp Lab, Ctr Informat Technol, Bethesda, MD 20892 USA. [Linehan, W. Marston] NCI, Urol Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Timmers, Henri J.] Radboud Univ Nijmegen, Dept Endocrinol, NL-6525 ED Nijmegen, Netherlands. [Tischler, Arthur S.; Powers, James F.] Tufts Med Ctr, Dept Pathol, Boston, MA USA. [de Krijger, Ronald] Erasmus MC Univ Med Ctr, Dept Pathol, Josephine Nefkens Inst, Rotterdam, Netherlands. [Baysal, Bora E.] Roswell Pk Canc Inst, Dept Pathol, Buffalo, NY 14263 USA. [Takacova, Martina; Pastorekova, Silvia] Slovak Acad Sci, Inst Virol, Bratislava, Slovakia. [Gius, David] Feinberg Northwestern Med Sch, Dept Radiat Oncol, Chicago, IL USA. RP Pacak, K (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Program Reprod & Adult Endocrinol, NIH, CRC, Bldg 10,1 East,Room 1-3140,10 Ctr Dr, Bethesda, MD 20892 USA. EM karel@mail.nih.gov FU Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Human Genome Research Institute, National Institutes of Health (Bethesda, MD) FX This study was funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the National Human Genome Research Institute, National Institutes of Health (Bethesda, MD). The authors have nothing to disclose. NR 55 TC 15 Z9 15 U1 0 U2 4 PU NEOPLASIA PRESS PI ANN ARBOR PA 1150 W MEDICAL CENTER DR, MSRB III, RM 9303, ANN ARBOR, MI 48109-0648 USA SN 1522-8002 J9 NEOPLASIA JI Neoplasia PD APR PY 2013 VL 15 IS 4 BP 435 EP + DI 10.1593/neo.122132 PG 16 WC Oncology SC Oncology GA 219DO UT WOS:000324486000008 PM 23555188 ER PT J AU Coleman, CN Blumenthal, DJ Casto, CA Alfant, M Simon, SL Remick, AL Gepford, HJ Bowman, T Telfer, JL Blumenthal, PM Noska, MA AF Coleman, C. Norman Blumenthal, Daniel J. Casto, Charles A. Alfant, Michael Simon, Steven L. Remick, Alan L. Gepford, Heather J. Bowman, Thomas Telfer, Jana L. Blumenthal, Pamela M. Noska, Michael A. TI Recovery and Resilience After a Nuclear Power Plant Disaster: A Medical Decision Model for Managing an Effective, Timely, and Balanced Response SO DISASTER MEDICINE AND PUBLIC HEALTH PREPAREDNESS LA English DT Article DE nuclear power plant; disaster resilience; disaster recovery; medical decision model ID CANCER-RISKS; HEALTH; JAPAN; FALLOUT AB Resilience after a nuclear power plant or other radiation emergency requires response and recovery activities that are appropriately safe, timely, effective, and well organized. Timely informed decisions must be made, and the logic behind them communicated during the evolution of the incident before the final outcome is known. Based on our experiences in Tokyo responding to the Fukushima Daiichi nuclear power plant crisis, we propose a real-time, medical decision model by which to make key health-related decisions that are central drivers to the overall incident management. Using this approach, on-site decision makers empowered to make interim decisions can act without undue delay using readily available and high-level scientific, medical, communication, and policy expertise. Ongoing assessment, consultation, and adaption to the changing conditions and additional information are additional key features. Given the central role of health and medical issues in all disasters, we propose that this medical decision model, which is compatible with the existing US National Response Framework structure, be considered for effective management of complex, large-scale, and large-consequence incidents. C1 [Coleman, C. Norman] NCI, Off Assistant Secretary Preparedness & Response, Radiat Res Program, Div Canc Treatment & Diag,NIH, Bethesda, MD 20892 USA. [Simon, Steven L.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Blumenthal, Daniel J.; Remick, Alan L.] Natl Nucl Secur Adm, Dept Energy, Washington, DC USA. [Casto, Charles A.; Gepford, Heather J.] Nucl Regulatory Commiss, Rockville, MD USA. [Alfant, Michael] Amer Chamber Commerce Japan, Tokyo, Japan. [Bowman, Thomas] Ctr Dis Control & Prevent, Div Strateg Natl Stockpile, Atlanta, GA USA. [Telfer, Jana L.] Ctr Dis Control & Prevent, Agcy Tox Subst & Dis Registry, Atlanta, GA USA. [Blumenthal, Pamela M.] Dept Housing & Urban Dev, Washington, DC USA. [Noska, Michael A.] US FDA, Silver Spring, MD USA. RP Coleman, CN (reprint author), 9609 Med Ctr Dr,Room 3W102, Rockville, MD 20850 USA. EM ccoleman@mail.nih.gov NR 33 TC 3 Z9 3 U1 0 U2 23 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1935-7893 J9 DISASTER MED PUBLIC JI Dis. Med. Public Health Prep. PD APR PY 2013 VL 7 IS 2 BP 136 EP 145 DI 10.1017/dmp.2013.5 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 200AF UT WOS:000323037100009 PM 24618164 ER PT J AU Yokoyama, S Perera, PY Waldmann, TA Hiroi, T Perera, LP AF Yokoyama, Seiji Perera, Pin-Yu Waldmann, Thomas A. Hiroi, Takachika Perera, Liyanage P. TI Tofacitinib, a Janus Kinase Inhibitor Demonstrates Efficacy in an IL-15 Transgenic Mouse Model that Recapitulates Pathologic Manifestations of Celiac Disease SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Article DE Celiac disease; IL-15; tofacitinib; JAK inhibitors AB Celiac disease (CD) is an immune-mediated, inflammatory disorder of the small intestines with a defined genetic etiological component associated with the expression of HLA-DQ2 and/or HLA-DQ8 haplotypes. The dietary consumption of gluten-rich cereals triggers a gluten-specific immune response in genetically susceptible individuals leading to a spectrum of clinical manifestations ranging from an inapparent subclinical disease, to overt enteropathy that can in some individuals progress to enteropathy-associated T cell lymphoma (EATL). The tissue-destructive pathologic process of CD is driven by activated NK-like intraepithelial CD8(+) lymphocytes and the proinflammatory cytokine IL-15 has emerged to be pivotal in orchestrating this perpetual tissue destruction and inflammation. Moreover, transgenic mice that over-express human IL-15 from an enterocyte-specific promoter (T3(b)-hIL-15 Tg) recapitulate many of the disease-defining T and B cell-mediated pathologic features of CD, further supporting the evolving consensus that IL-15 represents a valuable target in devising therapeutic interventions against the form of the disease that is especially refractory to gluten-free diet. In the present study, we evaluated the potential efficacy of tofacitinib, a pan-JAK inhibitor that abrogates IL-15 signaling, as a therapeutic modality against CD using T3(b)-hIL-15 Tg mice. We demonstrate that tofacitinib therapy leads to a lasting reversal of pathologic manifestations in the treated mice, thereby highlighting the potential value of tofacitininb as a therapeutic modality against refractory CD for which no effective therapy exists currently. Additionally, the visceral adiposity observed in the tofacitinib-treated mice underscores the importance of continued evaluation of the drug's impact on the lipid metabolism. C1 [Yokoyama, Seiji; Hiroi, Takachika] Tokyo Metropolitan Inst Med Sci, Dept Allergy & Immunol, Setagaya Ku, Tokyo 1568506, Japan. [Perera, Pin-Yu] Vet Affairs Med Ctr, Washington, DC 20422 USA. [Waldmann, Thomas A.; Perera, Liyanage P.] NCI, Metab Branch, Bethesda, MD 20892 USA. RP Hiroi, T (reprint author), Tokyo Metropolitan Inst Med Sci, Dept Allergy & Immunol, Setagaya Ku, 2-1-6 Kamikitazawa, Tokyo 1568506, Japan. EM hiroi-tk@igakuken.or.jp; pereral@mail.nih.gov FU MEXT in Japan from the Ministry of Education, Science, Sports, and Culture of Japan; Japan Society for the Promotion of Science; National Cancer Institute FX This research was supported in part by the intramural programs of the National Cancer Institute and a Grant-in-Aid for 2009 Multidisciplinary Research Project from MEXT in Japan from the Ministry of Education, Science, Sports, and Culture of Japan (T. Hiroi). L.P. Perera gratefully acknowledges the receipt of an invitational fellowship from the Japan Society for the Promotion of Science. NR 21 TC 18 Z9 18 U1 0 U2 4 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 586 EP 594 DI 10.1007/s10875-012-9849-y PG 9 WC Immunology SC Immunology GA 201EO UT WOS:000323123700010 PM 23269601 ER PT J AU Mizesko, M Banerjee, P Monaco-Shawver, L Mace, E Bernal, W Sawalle-Belohradsky, J Belohradsky, BH Heinz, V Freeman, AF Sullivan, KE Holland, SM Torgerson, T Al-Herz, W Chou, J Hanson, IC Albert, MH Geha, RS Renner, E Orange, J AF Mizesko, Melissa Banerjee, Pinaki Monaco-Shawver, Linda Mace, Emily Bernal, William Sawalle-Belohradsky, Julie Belohradsky, Bernd H. Heinz, Valerie Freeman, Alexandra F. Sullivan, Kathleen E. Holland, Steven M. Torgerson, Troy Al-Herz, Waleed Chou, Janet Hanson, Imelda Celine Albert, Michael H. Geha, Raif S. Renner, Ellen Orange, Jordan TI DOCK8 IS REQUIRED FOR ACTIN ACCUMULATION AT THE NK CELL LYTIC SYNAPSE AND NK CELL CYTOTOXIC FUNCTION SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Mizesko, Melissa; Banerjee, Pinaki; Hanson, Imelda Celine; Orange, Jordan] Texas Childrens Hosp, Houston, TX 77030 USA. [Mizesko, Melissa; Banerjee, Pinaki; Mace, Emily; Hanson, Imelda Celine; Orange, Jordan] Baylor Coll Med, Sect Immunol Allergy & Rheumatol, Houston, TX 77030 USA. [Monaco-Shawver, Linda; Bernal, William; Sullivan, Kathleen E.] Childrens Hosp Philadelphia, Res Inst, Philadelphia, PA USA. [Sawalle-Belohradsky, Julie; Belohradsky, Bernd H.; Heinz, Valerie; Albert, Michael H.; Renner, Ellen] Dr von Haunersches Kinderspital, Munich, Germany. [Freeman, Alexandra F.; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Torgerson, Troy] Univ Washington, Sch Med, Dept Pediat, Seattle, WA 98195 USA. [Torgerson, Troy] Seattle Childrens Hosp, Seattle, WA USA. [Al-Herz, Waleed] Kuwait Univ, Fac Med, Dept Pediat, Kuwait, Kuwait. [Chou, Janet; Geha, Raif S.] Harvard Univ, Childrens Hosp, Div Immunol, Sch Med, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 671 EP 671 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700021 ER PT J AU Spinner, MA Sanchez, LA Hsu, AP Calvo, K Cuellar-Rodriguez, J Hickstein, DD Zerbe, CS Holland, SM AF Spinner, Michael A. Sanchez, Lauren A. Hsu, Amy P. Calvo, Katherine Cuellar-Rodriguez, Jennifer Hickstein, Dennis D. Zerbe, Christa S. Holland, Steven M. TI GATA2 DEFICIENCY: EXTENDED CLINICAL PHENOTYPE IN 57 PATIENTS SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Spinner, Michael A.; Sanchez, Lauren A.; Cuellar-Rodriguez, Jennifer; Zerbe, Christa S.; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Hsu, Amy P.] NIAID, Lab Clin Infect Dis, Bethesda, MD 20892 USA. [Calvo, Katherine] NIH, Hematol Sect, Dept Lab Med, Ctr Clin, Bethesda, MD 20892 USA. [Hickstein, Dennis D.] NCI, Expt Transplantat & Immunol Branch, Div Basic Sci, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 672 EP 672 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700024 ER PT J AU Falcone, EL Marciano, BE Zerbe, CS Brinster, LR DeRavin, SS Malech, HL Heller, T Holland, SM AF Falcone, Emilia Liana Marciano, Beatriz E. Zerbe, Christa S. Brinster, Lauren R. DeRavin, Suk See Malech, Harry L. Heller, Theo Holland, Steven M. TI GASTROINTESTINAL MANIFESTATIONS IN AUTOSOMAL RECESSIVE CHRONIC GRANULOMATOUS DISEASE SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Falcone, Emilia Liana; Marciano, Beatriz E.; Zerbe, Christa S.; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Brinster, Lauren R.] NIH, Pathol Serv, Div Vet Resources, Bethesda, MD 20892 USA. [DeRavin, Suk See; Malech, Harry L.] NIAID, Host Def Lab, NIH, Bethesda, MD 20892 USA. [Heller, Theo] NIDDK, Liver Dis Branch, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 675 EP 675 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700030 ER PT J AU Sokolic, R Muul, L Brown, R Koh, C Manoli, E Garabedian, E Myles, J Venditti, C Heller, T Kleiner, D Hadigan, C Yanovski, J Candotti, F AF Sokolic, Robert Muul, Linda Brown, Rebecca Koh, Christopher Manoli, Eirini Garabedian, Elizabeth Myles, Jennifer Venditti, Charles Heller, Theo Kleiner, David, Jr. Hadigan, Colleen Yanovski, Jack Candotti, Fabio TI THE EXPANDING CLINICAL SPECTRUM OF ADENOSINE DEAMINASE (ADA) DEFICIENCY INCLUDES FEATURES OF METABOLIC SYNDROME SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Sokolic, Robert; Muul, Linda; Garabedian, Elizabeth; Candotti, Fabio] NHGRI, Disorders Immun Sect, Genet & Mol Biol Branch, Bethesda, MD 20892 USA. [Brown, Rebecca] NIDDK, Sect Pediat Diabet & Metab, Bethesda, MD USA. [Koh, Christopher] NIDDK, Gastroenterol Sect, Bethesda, MD USA. [Manoli, Eirini] NHGRI, Organ Acid Res Sect, Genet & Mol Biol Branch, Bethesda, MD 20892 USA. [Myles, Jennifer] NIH, Clin Nutr Serv, Warren Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA. [Venditti, Charles] NIH, Organ Acids Res Sect, Genet & Mol Biol Branch, Bethesda, MD 20892 USA. [Heller, Theo] NIDDK, Liver Dis Branch, Bethesda, MD USA. [Kleiner, David, Jr.] NCI, Pathol Lab, Bethesda, MD 20892 USA. [Hadigan, Colleen] NIAID, Clin Res Sect, Bethesda, MD 20892 USA. [Yanovski, Jack] NICHHD, Sect Growth & Obes, Program Dev Endocrinol & Genet, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 675 EP 676 PG 2 WC Immunology SC Immunology GA 201EO UT WOS:000323123700031 ER PT J AU Browne, SK Rosen, LB Freeman, AF Yang, L Jutivorakool, K Olivier, KN Angkasekwinai, N Suputtamongkol, Y Bennett, J Pyrgos, V Williamson, P Ding, L Holland, SM AF Browne, Sarah K. Rosen, Lindsey B. Freeman, Alexandra F. Yang, Lauren Jutivorakool, Kamonwan Olivier, Kenneth N. Angkasekwinai, Nasikarn Suputtamongkol, Yupin Bennett, John Pyrgos, Vasilios Williamson, Peter Ding, Li Holland, Steven M. TI ANTI-GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR AUTOANTIBODIES IN PATIENTS WITH CRYPTOCOCCAL MENINGITIS SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Browne, Sarah K.; Rosen, Lindsey B.; Freeman, Alexandra F.; Olivier, Kenneth N.; Ding, Li; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Yang, Lauren] Washington Univ, Sch Med, St Louis, MO 63130 USA. [Jutivorakool, Kamonwan; Bennett, John; Pyrgos, Vasilios; Williamson, Peter] NIAID, Bethesda, MD USA. [Jutivorakool, Kamonwan] Chulalongkorn Univ, Dept Med, Fac Med, Bangkok 10330, Thailand. [Angkasekwinai, Nasikarn; Suputtamongkol, Yupin] Mahidol Univ, Med 4Fac, Siriraj Hosp, Bangkok 10700, Thailand. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 677 EP 677 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700034 ER PT J AU Hanks, ME Kumar, N Davis, BC Van Wagoner, N Hsu, AP Merlin, JS La Hoz, R Chandrasekaran, P Zerbe, CS Holland, SM Sampaio, EP AF Hanks, Mary E. Kumar, Nilay Davis, Brian C. Van Wagoner, Nicholas Hsu, Amy P. Merlin, Jessica S. La Hoz, Ricardo Chandrasekaran, Prabha Zerbe, Christa S. Holland, Steven M. Sampaio, Elizabeth P. TI NOVEL GAIN OF FUNCTION MUTATION IN STAT1 ASSOCIATED WITH DISSEMINATED ZYGOMYCOSIS SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Hanks, Mary E.; Chandrasekaran, Prabha; Sampaio, Elizabeth P.] NIH, LCID, Bethesda, MD 20892 USA. [Hsu, Amy P.; Zerbe, Christa S.; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Kumar, Nilay; Davis, Brian C.] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA. [Van Wagoner, Nicholas; Merlin, Jessica S.; La Hoz, Ricardo] Univ Alabama Birmingham, Dept Med, Div Infect Dis, Birmingham, AL 35294 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 681 EP 681 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700045 ER PT J AU Wessel, AW Hsu, AP Zilberman-Rudenko, J Goldbach-Mansky, RT Uzel, G Hanson, EP AF Wessel, Alex W. Hsu, Amy P. Zilberman-Rudenko, Jevgenia Goldbach-Mansky, Raphaela T. Uzel, Gulbu Hanson, Eric P. TI A NOVEL MUTATION LEADS TO A NEMO SPLICE ISOFORM AND UNBALANCED NF-KB- AND IRF3-INDUCED GENE EXPRESSION SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Wessel, Alex W.; Zilberman-Rudenko, Jevgenia; Hanson, Eric P.] NIAMSD, Autoimmun Branch, Bethesda, MD 20892 USA. [Hsu, Amy P.; Uzel, Gulbu] NIAID, Lab Clin Infect Dis, Bethesda, MD 20892 USA. [Goldbach-Mansky, Raphaela T.] NIAMSD, Pediat Translat Res Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 687 EP 687 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700059 ER PT J AU Ray, M Wada, Y Diaz, M AF Ray, Madhumita Wada, Yoko Diaz, Marilyn TI DEVELOPMENT OF NOVEL MOUSE MODELS OF AUTOIMMUNE DISEASE AND FIBROSIS SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Ray, Madhumita; Wada, Yoko; Diaz, Marilyn] NIEHS, Somat Hypermutat Grp, Mol Genet Lab, NIH, Durham, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 689 EP 690 PG 2 WC Immunology SC Immunology GA 201EO UT WOS:000323123700067 ER PT J AU Chandrakasan, S Su, H Kumar, AR AF Chandrakasan, Shanmuganathan Su, Helen Kumar, Ashish R. TI DOCK-8 DEFICIENCY- A CASE OF COMBINED IMMUNODEFICIENCY, EOSINOPHILIA AND ATOPY WITHOUT ELEVATED SERUM IgE SO JOURNAL OF CLINICAL IMMUNOLOGY LA English DT Meeting Abstract C1 [Chandrakasan, Shanmuganathan] Cincinnati Childrens Hosp Med Ctr, Cincinnati, OH 45229 USA. [Su, Helen] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Kumar, Ashish R.] Cincinnati Childrens Hosp Med Ctr, Div Bone Marrow Transplantat & Immunodeficiency, Cincinnati, OH 45229 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0271-9142 J9 J CLIN IMMUNOL JI J. Clin. Immunol. PD APR PY 2013 VL 33 IS 3 BP 694 EP 694 PG 1 WC Immunology SC Immunology GA 201EO UT WOS:000323123700079 ER PT J AU Weintraub, DB Zaghloul, KA AF Weintraub, David B. Zaghloul, Kareem A. TI The role of the subthalamic nucleus in cognition SO REVIEWS IN THE NEUROSCIENCES LA English DT Article DE cognition; deep brain stimulation (DBS); subthalamic nucleus (STN) ID DEEP-BRAIN-STIMULATION; ADVANCED PARKINSON-DISEASE; HIGH-FREQUENCY STIMULATION; BASAL GANGLIA; NEURONAL-ACTIVITY; RESPONSE-INHIBITION; ACTION SELECTION; DECISION-MAKING; REACTION-TIME; OSCILLATORY ACTIVITY AB Because the complex functions of the basal ganglia have been increasingly studied over the past several decades, the understanding of the role of the subthalamic nucleus (STN) in motor and cognitive functions has evolved. The traditional role in motor function ascribed to the STN, based on its involvement in the cortico-striato-thalamo-cortical motor loops, the pathologic STN activity seen in Parkinson's disease, and the benefits in motor symptoms following STN lesions and deep brain stimulation, has been revised to include wider cognitive functions. The increased attention focused on such non-motor functions housed within the STN partially arose from the observed cognitive and affective side effects seen with STN deep brain stimulation. The multiple modalities of research have corroborated these findings and have provided converging evidence that the STN is critically involved in cognitive processes. In particular, numerous experiments have demonstrated the involvement of the STN in high-conflict decisions. The different STN functions appear to be related to activity in anatomically distinct subregions, with the ventral STN contributing to high-conflict decision-making through its role in the hyperdirect pathway involving the prefrontal cortex. C1 [Weintraub, David B.; Zaghloul, Kareem A.] NIH, Surg Neurol Branch, Bethesda, MD 20814 USA. RP Zaghloul, KA (reprint author), NIH, Surg Neurol Branch, 10 Ctr Dr,3D20, Bethesda, MD 20814 USA. EM kareem.zaghloul@nih.gov FU Intramural Research Program of the NIH, NINDS FX This research was supported by the Intramural Research Program of the NIH, NINDS. NR 84 TC 12 Z9 12 U1 5 U2 16 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0334-1763 J9 REV NEUROSCIENCE JI Rev. Neurosci. PD APR PY 2013 VL 24 IS 2 BP 125 EP 138 DI 10.1515/revneuro-2012-0075 PG 14 WC Neurosciences SC Neurosciences & Neurology GA 199CD UT WOS:000322971000002 PM 23327862 ER PT J AU Tian, F Zhan, F VanderKraats, ND Hiken, JF Edwards, JR Zhang, HM Zhao, KJ Song, JZ AF Tian, Fei Zhan, Fei VanderKraats, Nathan D. Hiken, Jeffrey F. Edwards, John R. Zhang, Huanmin Zhao, Keji Song, Jiuzhou TI DNMT gene expression and methylome in Marek's disease resistant and susceptible chickens prior to and following infection by MDV SO EPIGENETICS LA English DT Article DE epigenetics; DNA methylation; tumor; viral infection; Marek's disease; chicken ID DNA METHYLATION; TRANSPOSABLE ELEMENTS; EPIGENETIC REGULATION; CELL-LINES; CANCER; GENOME; VIRUS; METHYLTRANSFERASES; TRANSFORMATION; TRANSCRIPTION AB Marek's disease (MD) is characterized as a T cell lymphoma induced by a cell-associated alpha-herpesvirus, Marek's disease virus type 1 (MDV1). As with many viral infectious diseases, DNA methylation variations were observed in the progression of MD; these variations are thought to play an important role in host-virus interactions. We observed that DNA methyltransferase 3a (DNMT3a) and 3b (DNMT3b) were differentially expressed in chicken MD-resistant line 6(3) and MD-susceptible line 7(2) at 21 d after MDV infection. To better understand the role of methylation variation induced by MDV infection in both chicken lines, we mapped the genome-wide DNA methylation profiles in each line using Methyl-MAPS (methylation mapping analysis by paired-end sequencing). Collectively, the data sets collected in this study provide a more comprehensive picture of the chicken methylome. Overall, methylation levels were reduced in chickens from the resistant line 6(3) after MDV infection. We identified 11,512 infection-induced differential methylation regions (iDMRs). The number of iDMRs was larger in line 7(2) than in line 6(3), and most of iDMRs found in line 6(3) were overlapped with the iDMRs found in line 7(2). We further showed that in vitro methylation levels were associated with MDV replication, and found that MDV propagation in the infected cells was restricted by pharmacological inhibition of DNA methylation. Our results suggest that DNA methylation in the host may be associated with disease resistance or susceptibility. The methylation variations induced by viral infection may consequentially change the host transcriptome and result in diverse disease outcomes. C1 [Tian, Fei; Zhan, Fei; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [VanderKraats, Nathan D.; Hiken, Jeffrey F.; Edwards, John R.] Washington Univ, Sch Med, Dept Med, Ctr Pharmacogen, St Louis, MO 63110 USA. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI USA. [Zhang, Huanmin] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Zhao, Keji] NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. RP Edwards, JR (reprint author), Washington Univ, Sch Med, Dept Med, Ctr Pharmacogen, St Louis, MO 63110 USA. EM jedwards@dom.wustl.edu; songj88@umd.edu FU NLM NIH HHS [R21 LM011199] NR 40 TC 4 Z9 6 U1 1 U2 10 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1559-2294 J9 EPIGENETICS-US JI Epigenetics PD APR 1 PY 2013 VL 8 IS 4 BP 431 EP 444 DI 10.4161/epi.24361 PG 14 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 201XP UT WOS:000323176600011 PM 23538681 ER PT J AU Anstee, QM Darlay, R Leathart, J Liu, YL Tordjman, J Clement, K Aithal, G Valenti, L Van Gaal, L Stickel, F Alison, M Romero-Gomez, M Andrade, R Reeves, H Bedossa, P Pihlajamaki, J Kotronen, A Yki-Jarvinen, H Chalasani, N Kleiner, DE Rotter, J Burt, A Ratziu, V Cordell, HJ Daly, AK Day, CP AF Anstee, Q. M. Darlay, R. Leathart, J. Liu, Y. -L. Tordjman, J. Clement, K. Aithal, G. Valenti, L. Van Gaal, L. Stickel, F. Alison, M. Romero-Gomez, M. Andrade, R. Reeves, H. Bedossa, P. Pihlajamaki, J. Kotronen, A. Yki-Jarvinen, H. Chalasani, N. Kleiner, D. E. Rotter, J. Burt, A. Ratziu, V. Cordell, H. J. Daly, A. K. Day, C. P. CA FLIP Fatty Liver Inhibition NASH CRN Investigators TI A CANDIDATE-GENE APPROACH TO VALIDATION OF GENETIC MODIFIER ASSOCIATIONS USING A LARGE COHORT WITH HISTOLOGICALLY CHARACTERISED NON-ALCOHOLIC FATTY LIVER DISEASE SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Anstee, Q. M.; Leathart, J.; Liu, Y. -L.; Burt, A.; Daly, A. K.; Day, C. P.] Newcastle Univ, Inst Cellular Med, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Darlay, R.; Cordell, H. J.] Newcastle Univ, Inst Med Genet, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Tordjman, J.; Clement, K.] Hop La Pitie Salpetriere, ICAN Inst Cardiometab & Nutr, Paris, France. [Aithal, G.] NIHR BRU, Nottingham Digest Dis Ctr, Biomed Res Unit, Nottingham, England. [Valenti, L.] Univ Milan, Milan, Italy. [Van Gaal, L.] Univ Antwerp Hosp, Dept Endocrinol Diabetol & Metab, Antwerp, Belgium. [Stickel, F.] Univ Bern, Bern, Switzerland. [Alison, M.] Cambridge Univ Hosp, Cambridge, England. [Romero-Gomez, M.] Valme Univ Hosp, Seville, Spain. [Andrade, R.] Univ Virgen Victoria, Fac Med, Malaga, Spain. [Reeves, H.] Newcastle Univ, Northern Inst Canc Res, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Bedossa, P.] Beaujon Hosp, Paris, France. [Pihlajamaki, J.] Univ Eastern Finland, Kuopio, Finland. [Kotronen, A.; Yki-Jarvinen, H.] Univ Helsinki, Helsinki, Finland. [Chalasani, N.] Indiana Univ Sch Med, Indianapolis, IN 46202 USA. [Kleiner, D. E.] NCI, Bethesda, MD 20892 USA. [Rotter, J.] Cedars Sinai Genet Inst, Los Angeles, CA USA. [Ratziu, V.] Univ Paris 06, Paris, France. EM quentin.anstee@newcastle.ac.uk RI Romero Gomez, Manuel/L-8030-2014; Valenti, Luca/B-3695-2009 OI Valenti, Luca/0000-0001-8909-0345 NR 0 TC 6 Z9 6 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 104 BP S46 EP S46 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000105 ER PT J AU Bajaj, JS Sheikh, MY Chojkier, M Balart, L Sherker, AH Vemuru, R Sussman, NL Vierling, J Morelli, G Anderson, KE Harris, MS Mullen, KD AF Bajaj, J. S. Sheikh, M. Y. Chojkier, M. Balart, L. Sherker, A. H. Vemuru, R. Sussman, N. L. Vierling, J. Morelli, G. Anderson, K. E. Harris, M. S. Mullen, K. D. TI AST-120 (SPHERICAL CARBON ADSORBENT) IN COVERT HEPATIC ENCEPHALOPATHY: RESULTS OF THE ASTUTE TRIAL SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Bajaj, J. S.] McGuire DVAMC, Richmond, VA USA. [Sheikh, M. Y.] UCSF, Fresno Community Reg Med Ctr, Fresno, CA USA. [Chojkier, M.] Ctr Vet Med, San Diego, CA USA. [Balart, L.] Tulane Univ, Hlth Sci Ctr, New Orleans, LA 70118 USA. [Sherker, A. H.] NIDDK, NIH, Bethesda, MD USA. [Vemuru, R.] Permian Res Fdn, Odessa, TX USA. [Sussman, N. L.; Vierling, J.] Baylor Coll Med, Houston, TX 77030 USA. [Morelli, G.] Univ Florida, Gainesville, FL USA. [Anderson, K. E.] Ocera Therapeut Inc, San Diego, CA USA. [Harris, M. S.] Georgetown Univ, Sch Med, Georgetown, DC USA. [Mullen, K. D.] Case Western Reserve MetroHlth Med Ctr, Cleveland, OH USA. EM jasmohan.bajaj@va.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 190 BP S84 EP S84 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000191 ER PT J AU Barrett, L Shivasabesan, G Wang, C Osinusi, A Kohli, A Meissner, EG Heytens, L Nelson, A Polis, MA Masur, H Symonds, W McHutchinson, J Kottilil, S AF Barrett, L. Shivasabesan, G. Wang, C. Osinusi, A. Kohli, A. Meissner, E. G. Heytens, L. Nelson, A. Polis, M. A. Masur, H. Symonds, W. McHutchinson, J. Kottilil, S. TI ALTERED HCV SPECIFIC T CELL IMMUNITY VERY EARLY IN INTERFERON FREE HCV DAA THERAPY SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Barrett, L.; Shivasabesan, G.; Wang, C.; Osinusi, A.; Kohli, A.; Meissner, E. G.; Nelson, A.; Polis, M. A.; Kottilil, S.] NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. [Osinusi, A.; Kohli, A.] SAIC Frederick Inc, Frederick, MD USA. [Heytens, L.; Masur, H.] NIH, CCMD, CC, Bethesda, MD 20892 USA. [Symonds, W.; McHutchinson, J.] Gilead Sci Inc, Foster City, CA 94404 USA. EM barrettl@niaid.nih.gov NR 0 TC 4 Z9 4 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 1 BP S1 EP S1 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000002 ER PT J AU Bon, D Herrmann, E Shivakumar, B Lee, YJ Heytens, L Polis, M Masur, H Neumann, AU Symonds, WT McHutchison, J Kottilil, S Osinusi, A AF Bon, D. Herrmann, E. Shivakumar, B. Lee, Y. -J. Heytens, L. Polis, M. Masur, H. Neumann, A. U. Symonds, W. T. McHutchison, J. Kottilil, S. Osinusi, A. TI PK-PD MODELING IN A SUB-GROUP OF PATIENTS TREATED WITH GS-7977 AND RIBAVIRIN SHOWED A SIGNIFICANTLY SLOWER EARLY VIRAL KINETICS IN RELAPSERS SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Bon, D.; Herrmann, E.] Goethe Univ Frankfurt, Fac Med, Inst Biostat & Math Modelling, D-60054 Frankfurt, Germany. [Shivakumar, B.; Lee, Y. -J.; Heytens, L.; Polis, M.; Kottilil, S.; Osinusi, A.] NIAID, NIH, Bethesda, MD 20892 USA. [Masur, H.] NIH, CCMD, CC, Bethesda, MD 20892 USA. [Neumann, A. U.] Humboldt Univ, Inst Theoret Biol, D-10099 Berlin, Germany. [Neumann, A. U.] Bar Ilan Univ, Goodman Fac Life Sci, Bar Ilan, Israel. [Symonds, W. T.; McHutchison, J.] Gilead Sci Inc, Foster City, CA 94404 USA. [Osinusi, A.] SAIC Frederick Inc, Clin Res Directorate, CMRP, Frederick Natl Lab Canc Res, Frederick, MD USA. EM bon@med.uni-frankfurt.de NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 1187 BP S483 EP S483 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001447 ER PT J AU Dhanda, A Liu, B Lee, R Collins, P AF Dhanda, A. Liu, B. Lee, R. Collins, P. TI CD14(+) CD16(+) MONOCYTES ARE EXPANDED IN PATIENTS WITH SEVERE ALCOHOLIC HEPATITIS AND CHARACTERISED BY HIGH EXPRESSION OF INTERLEUKIN 10 SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Dhanda, A.; Lee, R.] Univ Bristol, Sch Clin Sci, Bristol, Avon, England. [Dhanda, A.; Collins, P.] Univ Hosp Bristol NHS Fdn Trust, Dept Liver Med, Bristol, Avon, England. [Liu, B.] NEI, NIH, Bethesda, MD 20892 USA. EM ashwin.dhanda@bristol.ac.uk NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 523 BP S214 EP S215 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000524 ER PT J AU Kapelusznik, L Majid, AM Cox, ER Funk, E Flores, Y Charon, B Myers, J Dickson, C Knodell, RG El-Kamary, S Talwani, R AF Kapelusznik, L. Majid, A. M. Cox, E. R. Funk, E. Flores, Y. Charon, B. Myers, J. Dickson, C. Knodell, R. G. El-Kamary, S. Talwani, R. TI BOCEPREVIR IS ASSOCIATED WITH DECLINE IN RENAL FUNCTION IN A COHORT OF HCV-TREATED PATIENTS SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Kapelusznik, L.; Majid, A. M.; Cox, E. R.; El-Kamary, S.; Talwani, R.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. [Funk, E.] NIAID, NIH, Bethesda, MD 20892 USA. [Flores, Y.; Charon, B.; Myers, J.; Dickson, C.; Knodell, R. G.] Baltimore Vet Affairs Med Ctr, Baltimore, MD USA. EM lkapelusznik@ihv.umaryland.edu NR 0 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 834 BP S342 EP S342 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001096 ER PT J AU Lepiller, Q Soulier, E Lambotin, M Barths, J Bachellier, P Stoll-Keller, F Baumert, TF Liang, TJ Barth, H AF Lepiller, Q. Soulier, E. Lambotin, M. Barths, J. Bachellier, P. Stoll-Keller, F. Baumert, T. F. Liang, T. J. Barth, H. TI HUMAN HEPATOCYTE INDOLEAMINE-2,3-DIOXYGENASE CONTRIBUTES TO ANTIVIRAL DEFENSE AND IMMUNE REGULATION IN HEPATITIS C VIRUS INFECTION SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Lepiller, Q.; Soulier, E.; Lambotin, M.; Barths, J.; Stoll-Keller, F.; Baumert, T. F.; Barth, H.] INSERM, U748, Strasbourg, France. [Lepiller, Q.; Soulier, E.; Lambotin, M.; Barths, J.; Stoll-Keller, F.; Baumert, T. F.; Barth, H.] Univ Strasbourg, Strasbourg, France. [Lepiller, Q.; Stoll-Keller, F.; Barth, H.] Hop Univ Strasbourg, Inst Virol, Strasbourg, France. [Bachellier, P.] Hop Univ Strasbourg, Pole Pathol Digest Hepat & Transplantat, Strasbourg, France. [Baumert, T. F.] Hop Univ Strasbourg, Pole Hepatodigestif, Strasbourg, France. [Liang, T. J.] NIDDK, Liver Dis Branch, NIH, Bethesda, MD USA. EM lepiller@unistra.fr NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 343 BP S143 EP S143 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000344 ER PT J AU Marquardt, JU Ploch, P Becker, D Andersen, JB Seidel, D Eickmeier, I Gamstatter, T Hansen, T Conner, LA Galle, PR Schott, E Thorgeirsson, SS Teufel, A AF Marquardt, J. U. Ploch, P. Becker, D. Andersen, J. B. Seidel, D. Eickmeier, I. Gamstaetter, T. Hansen, T. Conner, L. A. Galle, P. R. Schott, E. Thorgeirsson, S. S. Teufel, A. TI IDENTIFICATION OF TWO CLINICALLY DISTINCT SUBTYPES IN AUTOIMMUNE HEPATITIS BY COMPARATIVE FUNCTIONAL GENOMICS SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Marquardt, J. U.; Ploch, P.; Becker, D.; Gamstaetter, T.; Galle, P. R.; Teufel, A.] Johannes Gutenberg Univ Mainz, Dept Med 1, Mainz, Germany. [Marquardt, J. U.; Andersen, J. B.; Conner, L. A.; Thorgeirsson, S. S.] NCI, Expt Carcinogenesis Lab, CCR, NIH, Bethesda, MD 20892 USA. [Seidel, D.; Eickmeier, I.; Schott, E.] Charite, CVK, Dept Hepatol & Gastroenterol, D-13353 Berlin, Germany. [Hansen, T.] Johannes Gutenberg Univ Mainz, Inst Pathol, Mainz, Germany. EM marquarj@uni-mainz.de NR 0 TC 0 Z9 0 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 949 BP S391 EP S391 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001210 ER PT J AU Meissner, EG Bon, D Osinusi, A Tang, W Herrmann, E McHutchison, J Symonds, W Masur, H O'Brien, TR Prokunina-Olsson, L Kottilil, S AF Meissner, E. G. Bon, D. Osinusi, A. Tang, W. Herrmann, E. McHutchison, J. Symonds, W. Masur, H. O'Brien, T. R. Prokunina-Olsson, L. Kottilil, S. TI INVERSE CHANGES IN HEPATIC EXPRESSION OF INTERFERON LAMBDA AND ALPHA AND IFNL4 GENOTYPE ARE ASSOCIATED WITH TREATMENT RESPONSE IN SOFOSBUVIR/RIBAVIRIN TREATED HCV GENOTYPE-1 SUBJECTS SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Meissner, E. G.; Osinusi, A.; Kottilil, S.] NIAID, Lab Immunoregulat, NIH, Bethesda, MD 20892 USA. [Bon, D.; Herrmann, E.] Goethe Univ Frankfurt, Inst Biostat & Math Modeling, D-60054 Frankfurt, Germany. [Osinusi, A.] SAIC Frederick Inc, Clin Res Directorate CMRP, Frederick, MD USA. [Tang, W.; O'Brien, T. R.; Prokunina-Olsson, L.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [McHutchison, J.; Symonds, W.] Gilead Sci Inc, Foster City, CA 94404 USA. [Masur, H.] NIH, Dept Crit Care Med, Bethesda, MD 20892 USA. EM eric.meissner@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 1428 BP S575 EP S576 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001686 ER PT J AU O'Brien, T Pfeiffer, RM Kuniholm, MH Muchmore, B Aka, P Tang, W Chen, S Wang, A Astemborski, J Bonkovsky, HL Edlin, BR Howell, CD Morgan, TR Sharp, GB Strickler, HD Thomas, DL Prokunina-Olsson, L AF O'Brien, T. Pfeiffer, R. M. Kuniholm, M. H. Muchmore, B. Aka, P. Tang, W. Chen, S. Wang, A. Astemborski, J. Bonkovsky, H. L. Edlin, B. R. Howell, C. D. Morgan, T. R. Sharp, G. B. Strickler, H. D. Thomas, D. L. Prokunina-Olsson, L. TI A FRAME-SHIFT VARIANT IN THE NOVEL IFNL4 GENE IS ASSOCIATED WITH IMPAIRED HCV CLEARANCE SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [O'Brien, T.; Pfeiffer, R. M.; Muchmore, B.; Aka, P.; Tang, W.; Wang, A.; Prokunina-Olsson, L.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Kuniholm, M. H.; Strickler, H. D.] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10467 USA. [Chen, S.] IMS, Silver Spring, MD USA. [Astemborski, J.; Thomas, D. L.] Johns Hopkins Sch Hyg & Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Bonkovsky, H. L.] Carolinas Med Ctr, Dept Med, Charlotte, NC 28203 USA. [Edlin, B. R.] Natl Dev & Res Inst Inc, New York, NY USA. [Howell, C. D.] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Morgan, T. R.] VA Long Beach Healthcare Syst, Long Beach, CA USA. [Sharp, G. B.] NIAID, Div Aids, Bethesda, MD 20892 USA. [Thomas, D. L.] Johns Hopkins Univ, Div Infect Dis, Baltimore, MD USA. EM obrient@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 2 BP S1 EP S2 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983000003 ER PT J AU Parvez, MK Emerson, SU AF Parvez, M. K. Emerson, S. U. TI MOLECULAR CHARACTERIZATION OF HEPATITIS E VIRUS (HEV)-ORF1 SUPPORTS A PAPAIN-LIKE CYSTEINE PROTEASE (PCP) AND IDENTIFIES A PUTATIVE 'Gly-Gly-Gly' SUBSTRATE SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Parvez, M. K.] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh, Saudi Arabia. [Parvez, M. K.; Emerson, S. U.] NIAID, Mol Hepatitis Sect, LID, NIH, Bethesda, MD 20892 USA. EM khalid_parvez@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 1238 BP S502 EP S502 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001498 ER PT J AU Raggi, C Factor, VM Daekwan, S Matthew, GC Holczbauer, A Marquardt, JU Thorgeirsson, SS AF Raggi, C. Factor, V. M. Daekwan, S. Matthew, G. C. Holczbauer, A. Marquardt, J. U. Thorgeirsson, S. S. TI MECHANIC-BASED REPROGRAMMING OF HEPATIC SELF-RENEWING TUMOR-INITIATING CELLS THROUGH DNMT1-INHIBITION SO JOURNAL OF HEPATOLOGY LA English DT Meeting Abstract CT International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL) CY APR 24-28, 2013 CL Amsterdam, NETHERLANDS SP European Assoc Study Liver (EASL) C1 [Raggi, C.; Factor, V. M.; Daekwan, S.; Matthew, G. C.; Holczbauer, A.; Marquardt, J. U.; Thorgeirsson, S. S.] NCI, LEC, NIH, Bethesda, MD 20892 USA. EM chiara.raggi@humanitasresearch.it NR 0 TC 0 Z9 0 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 J9 J HEPATOL JI J. Hepatol. PD APR PY 2013 VL 58 SU 1 MA 1078 BP S441 EP S442 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 199GQ UT WOS:000322983001338 ER PT J AU Choi, BD Pastan, I Bigner, DD Sampson, JH AF Choi, Bryan D. Pastan, Ira Bigner, Darell D. Sampson, John H. TI A novel bispecific antibody recruits T cells to eradicate tumors in the "immunologically privileged" central nervous system SO ONCOIMMUNOLOGY LA English DT Article DE bispecific antibodies; central nervous system neoplasms; epidermal growth factor receptor; immunotherapy; T lymphocytes ID IMMUNOTHERAPY; GLIOMA AB Bispecific T-cell engagers (BiTEs) may break multiple barriers that currently limit the use of immunotherapy in glioblastoma patients. We have recently described a novel BiTE specific for a mutated form of the epidermal growth factor receptor, EGFRvIII, that exerts potent antineoplastic effects against established invasive tumors of the brain. C1 [Choi, Bryan D.; Bigner, Darell D.; Sampson, John H.] Duke Univ, Med Ctr, Dept Surg, Duke Brain Tumor Immunotherapy Program,Div Neuros, Durham, NC 27710 USA. [Choi, Bryan D.; Bigner, Darell D.; Sampson, John H.] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA. [Choi, Bryan D.; Bigner, Darell D.; Sampson, John H.] Duke Univ, Med Ctr, Preston Robert Tisch Brain Tumor Ctr Duke, Durham, NC USA. [Pastan, Ira] NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Sampson, JH (reprint author), Duke Univ, Med Ctr, Dept Surg, Duke Brain Tumor Immunotherapy Program,Div Neuros, Durham, NC 27710 USA. EM john.sampson@duke.edu NR 10 TC 7 Z9 7 U1 0 U2 5 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 2162-4011 J9 ONCOIMMUNOLOGY JI OncoImmunology PD APR 1 PY 2013 VL 2 IS 4 AR e23639 DI 10.4161/onci.23639 PG 2 WC Oncology; Immunology SC Oncology; Immunology GA 193NJ UT WOS:000322566200019 ER PT J AU Long, AH Haso, WM Orentas, RJ AF Long, Adrienne H. Haso, Waleed M. Orentas, Rimas J. TI Lessons learned from a highly-active CD22-specific chimeric antigen receptor SO ONCOIMMUNOLOGY LA English DT Article DE adoptive immunotherapy; CD22; leukemia; ALL; retroviral vectors ID IMMUNOTOXIN; INCREASE; CELLS; CD19 AB CD22 is an attractive target for the development of immunotherapeutic approaches for the therapy of B-cell malignancies. In particular, an m971 antibody-derived, second generation chimeric antigen receptor (CAR) that targets CD22 holds significant therapeutic promise. The key aspect for the development of such a highly-active CAR was its ability to target a membrane-proximal epitope of CD22. C1 [Long, Adrienne H.; Haso, Waleed M.; Orentas, Rimas J.] NCI, Pediat Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Orentas, RJ (reprint author), NCI, Pediat Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM Rimas.Orentas@nih.gov NR 10 TC 8 Z9 9 U1 0 U2 2 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 2162-4011 J9 ONCOIMMUNOLOGY JI OncoImmunology PD APR 1 PY 2013 VL 2 IS 4 AR e23621 DI 10.4161/onci.23621 PG 3 WC Oncology; Immunology SC Oncology; Immunology GA 193NJ UT WOS:000322566200033 ER PT J AU Chesebro, B Rangel, A Striebel, J Race, B AF Chesebro, Bruce Rangel, Alejandra Striebel, James Race, Brent TI Multiple effects of prion protein membrane anchoring on prion disease pathogenesis: Interaction of PrPres amyloid with the brain interstitial fluid (ISF) drainage pathway SO PRION LA English DT Meeting Abstract C1 [Chesebro, Bruce; Rangel, Alejandra; Striebel, James; Race, Brent] NIAID, Persistent Viral Dis Lab, Rocky Mt Labs, NIH, Hamilton, MT USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 6 EP 6 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500014 ER PT J AU Christiansen, JR Saijo, E Kim, S Kreeger, TJ Telling, GC AF Christiansen, Jeffrey R. Saijo, Eri Kim, Sehun Kreeger, Terry J. Telling, Glenn C. TI Moose chronic wasting disease is efficiently transmitted to animals expressing deer and elk prion protein SO PRION LA English DT Meeting Abstract C1 [Christiansen, Jeffrey R.; Kim, Sehun; Telling, Glenn C.] Colorado State Univ, Ft Collins, CO 80523 USA. [Saijo, Eri] NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, Hamilton, MT 59840 USA. [Kreeger, Terry J.] Wyoming Game & Fish Dept, Wheatland, WY USA. NR 4 TC 0 Z9 0 U1 0 U2 6 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 12 EP 12 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500028 ER PT J AU Henderson, DM Manca, M Haley, NJ Denkers, ND Nalls, AV Mathiason, CK Caughey, B Hoover, EA AF Henderson, Davin M. Manca, Matteo Haley, Nicholas J. Denkers, Nathaniel D. Nalls, Amy V. Mathiason, Candace K. Caughey, Byron Hoover, Edward A. TI Rapid and sensitive antemortem detection of CWD prions in saliva of deer by RT-QuIC SO PRION LA English DT Meeting Abstract C1 [Manca, Matteo; Caughey, Byron] NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, Hamilton, MT 59840 USA. [Henderson, Davin M.; Haley, Nicholas J.; Denkers, Nathaniel D.; Nalls, Amy V.; Mathiason, Candace K.; Hoover, Edward A.] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RI Mathiason, Candace/E-7843-2017 OI Mathiason, Candace/0000-0002-5920-4556 NR 0 TC 0 Z9 0 U1 0 U2 5 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 12 EP 13 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500029 ER PT J AU Elder, A Henderson, D Selariu, A Nalls, A Caughey, B Bessen, R Bartz, J Mathiason, C AF Elder, Alan Henderson, Davin Selariu, Anca Nalls, Amy Caughey, Byron Bessen, Richard Bartz, Jason Mathiason, Candace TI Detecting prions in the brain and blood of TSE-infected deer and hamsters SO PRION LA English DT Meeting Abstract C1 [Elder, Alan; Henderson, Davin; Selariu, Anca; Nalls, Amy; Bessen, Richard; Mathiason, Candace] Colorado State Univ, Ft Collins, CO 80523 USA. [Caughey, Byron] NIH, Rocky Mt Labs, Hamilton, MT USA. [Bartz, Jason] Creighton Univ, Omaha, NE 68178 USA. RI Mathiason, Candace/E-7843-2017 OI Mathiason, Candace/0000-0002-5920-4556 NR 0 TC 0 Z9 0 U1 0 U2 3 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 20 EP 20 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500045 ER PT J AU Hoon-Hanks, LL Haley, NJ Henderson, D Carver, S Caughey, B Hoover, EA AF Hoon-Hanks, Laura L. Haley, Nicholas J. Henderson, Davin Carver, Scott Caughey, Byron Hoover, Edward A. TI Analysis of cervid field samples byt RT-QuIC: Potential for improved sensitivity in surveillance and modeling SO PRION LA English DT Meeting Abstract C1 [Hoon-Hanks, Laura L.; Haley, Nicholas J.; Henderson, Davin; Hoover, Edward A.] Colorado State Univ, Ft Collins, CO 80523 USA. [Carver, Scott] Univ Tasmania, Hobart, Tas, Australia. [Caughey, Byron] NIH, Rocky Mt Labs, Hamilton, MT USA. RI Carver, Scott/J-7654-2014 OI Carver, Scott/0000-0002-3579-7588 NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 21 EP 21 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500048 ER PT J AU Kraus, A Race, B Phillips, K Groveman, B Kurnellas, M Rothbard, JB Steinman, L Caughey, B AF Kraus, Allison Race, Brent Phillips, Katie Groveman, Bradley Kurnellas, Michael Rothbard, Jonathan B. Steinman, Lawrence Caughey, Byron TI An investigation into the role of the prion protein in experimental autoimmune encephalomyelitis SO PRION LA English DT Meeting Abstract C1 [Kraus, Allison; Race, Brent; Phillips, Katie; Groveman, Bradley; Caughey, Byron] NIAID, Rocky Mt Labs, Hamilton, MT 59840 USA. Stanford, Palo Alto, CA USA. [Kurnellas, Michael; Rothbard, Jonathan B.; Steinman, Lawrence] Stanford Univ, Sch Med, Palo Alto, CA 94304 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 33 EP 33 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500074 ER PT J AU Hollister, JR Lee, KS Baron, GS AF Hollister, Jason R. Lee, Kil Sun Baron, Gerald S. TI Efficient uptake and dissemination of PrPres by astrocytes and fibroblasts derived from adult hamster brain SO PRION LA English DT Meeting Abstract C1 [Hollister, Jason R.; Lee, Kil Sun; Baron, Gerald S.] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 36 EP 36 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500080 ER PT J AU Carroll, JA Race, B Phillips, K Chesebro, B AF Carroll, James A. Race, Brent Phillips, Katie Chesebro, Bruce TI Temporal transcriptional analysis of genes mediating the inflammatory response in brain from scrapie-infected mice SO PRION LA English DT Meeting Abstract C1 [Carroll, James A.; Race, Brent; Phillips, Katie; Chesebro, Bruce] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 38 EP 38 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500084 ER PT J AU Choi, YP Priola, SA AF Choi, Young Pyo Priola, Suzette A. TI Uptake and disaggregation of scrapie prion protein in Prnp(-/-) neuronal cells SO PRION LA English DT Meeting Abstract C1 [Choi, Young Pyo; Priola, Suzette A.] NIAIA, Rocky Mt Labs, NIH, Hamilton, MT USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 38 EP 38 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500085 ER PT J AU Priola, SA Sturdevant, D Meade-White, K Chesebro, B Moore, RA AF Priola, Suzette A. Sturdevant, Dan Meade-White, Kimberly Chesebro, Bruce Moore, Roger A. TI Different cellular pathways are involved in the neuropathogenesis of amyloid and non-amyloid variants of mouse prion disease SO PRION LA English DT Meeting Abstract C1 [Priola, Suzette A.; Sturdevant, Dan; Meade-White, Kimberly; Chesebro, Bruce; Moore, Roger A.] NIAID, Rocky Mt Labs, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 45 EP 45 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500103 ER PT J AU Rangel, AH Race, B Chesebro, B Striebel, J AF Rangel, Alejandra H. Race, Brent Chesebro, Bruce Striebel, James TI Clearance of macromolecules from brain parenchyma differ in non-amyloid and amyloid prion diseases SO PRION LA English DT Meeting Abstract C1 [Rangel, Alejandra H.; Race, Brent; Chesebro, Bruce; Striebel, James] NIH, Rocky Mt Labs, Hamilton, MT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 46 EP 46 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500105 ER PT J AU Striebel, JF Race, B Pathmajeyan, M Rangel, A Chesebro, B AF Striebel, James F. Race, Brent Pathmajeyan, Melissa Rangel, Alejandra Chesebro, Bruce TI Minimal influence of prion protein gene expression on kainate-induced seizure susceptibility in mice SO PRION LA English DT Meeting Abstract C1 [Striebel, James F.; Race, Brent; Pathmajeyan, Melissa; Rangel, Alejandra; Chesebro, Bruce] NIAID, NIH, Rocky Mt Labs, Hamilton, MT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 47 EP 48 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500110 ER PT J AU Orru, CD Bongianni, M Tonoli, G Ferrari, S Hughson, A Pocchiari, M Monaco, S Caughey, B Zanusso, G AF Orru, Christina D. Bongianni, Matilde Tonoli, Giovanni Ferrari, Sergio Hughson, Andrew Pocchiari, Maurizio Monaco, Salvatore Caughey, Byron Zanusso, Gianluigi TI RT-QuIC of olfactory neuroepithelium brushings as a potential definitive intravital diagnosis of sporadic Creutzfeldt-Jakob disease SO PRION LA English DT Meeting Abstract C1 [Orru, Christina D.] Univ Cagliari, Monserrato, Italy. [Bongianni, Matilde; Tonoli, Giovanni; Ferrari, Sergio; Monaco, Salvatore; Zanusso, Gianluigi] Univ Verona, I-37100 Verona, Italy. [Orru, Christina D.; Bongianni, Matilde; Hughson, Andrew; Caughey, Byron] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. [Pocchiari, Maurizio] Ist Super Sanita, I-00161 Rome, Italy. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 56 EP 56 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500130 ER PT J AU Marshall, KE AF Marshall, Karen E. TI Transmembrane anchoring of the prion protein in a Prnp knockout cell line: The effects of plasma membrane location on propagation of misfolded PrP SO PRION LA English DT Meeting Abstract C1 [Marshall, Karen E.] NIAID, Rocky Mt Labs, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 86 EP 87 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500197 ER PT J AU Groveman, BR Caughey, B AF Groveman, Bradley R. Caughey, Byron TI Exploring the mechanics of prion conversion using RT-QuIC SO PRION LA English DT Meeting Abstract C1 [Groveman, Bradley R.; Caughey, Byron] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 96 EP 96 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500219 ER PT J AU Vieira, TC Caughey, B Silva, JL AF Vieira, Tuane C. Caughey, Byron Silva, Jerson L. TI Heparin modulation of prion seeding activity SO PRION LA English DT Meeting Abstract DE prion; gycosaminoglycan; aggregation; neurodegeneration C1 [Vieira, Tuane C.; Silva, Jerson L.] Univ Fed Rio de Janeiro, Rio De Janeiro, RJ, Brazil. [Caughey, Byron] NIH, Hamilton, MT USA. RI Silva, Jerson/J-8984-2014 OI Silva, Jerson/0000-0001-9523-9441 NR 1 TC 0 Z9 0 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-MAY PY 2013 VL 7 SU S BP 101 EP 101 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 202LL UT WOS:000323217500229 ER PT J AU Shiloh, S Wade, CH Roberts, JS Alford, SH Biesecker, BB AF Shiloh, Shoshana Wade, Christopher H. Roberts, J. Scott Alford, Sharon Hensley Biesecker, Barbara B. TI Associations between risk perceptions and worry about common diseases: A between- and within-subjects examination SO PSYCHOLOGY & HEALTH LA English DT Article DE risk perception; worry; severity; likelihood; within-subjects ID BREAST-CANCER SUSCEPTIBILITY; PERCEIVED RISK; PSYCHOLOGICAL ADJUSTMENT; ILLNESS REPRESENTATIONS; UNREALISTIC OPTIMISM; PROTECTIVE BEHAVIOR; PLANNED BEHAVIOR; FAMILY-HISTORY; UNITED-STATES; HEART-DISEASE AB The relationships between worry and perceptions of likelihood and severity were evaluated across eight common diseases. Individual and disease variability in worry and perceptions were examined. 294 participants were recruited through the Multiplex Initiative, in which a genetic susceptibility test for eight common diseases was offered to healthy adults. Participants completed a baseline telephone survey and web-based surveys without a commitment to be tested, and then made a choice on testing. Between- and within-subjects analyses yielded the following main findings: (1) worry is more closely related to likelihood perceptions than to severity perceptions; (2) severity perceptions add significantly to explained worry variances above and beyond likelihood perceptions; (3) risk perceptions and worries form two clusters: cancer diseases and cardiovascular-metabolic diseases; and (4) variance in risk perception and worry is explained by a combination of between- and within-subjects variances. Risk perception research should attend to severity perceptions, within-subjects variability and inter-disease differences, and to strategies for grouping conditions. C1 [Shiloh, Shoshana] Tel Aviv Univ, Sch Psychol Sci, IL-69978 Tel Aviv, Israel. [Wade, Christopher H.] Univ Washington, Dept Nursing, Bothell, WA USA. [Roberts, J. Scott] Univ Michigan, Sch Publ Hlth, Dept Hlth Behav & Hlth Educ, Ann Arbor, MI 48109 USA. [Alford, Sharon Hensley] Henry Ford Hlth Syst, Dept Biostat & Res Epidemiol, Detroit, MI USA. [Biesecker, Barbara B.] NHGRI, Social & Behav Res Branch, NIH, Bethesda, MD 20892 USA. RP Shiloh, S (reprint author), Tel Aviv Univ, Sch Psychol Sci, IL-69978 Tel Aviv, Israel. EM shoshi@freud.tau.ac.il FU Intramural NIH HHS [Z99 HG999999]; NCI NIH HHS [U19 CA079689, U19CA 079689] NR 73 TC 6 Z9 7 U1 0 U2 13 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0887-0446 J9 PSYCHOL HEALTH JI Psychol. Health PD APR 1 PY 2013 VL 28 IS 4 BP 434 EP 449 DI 10.1080/08870446.2012.737464 PG 16 WC Public, Environmental & Occupational Health; Psychology, Multidisciplinary SC Public, Environmental & Occupational Health; Psychology GA 194KN UT WOS:000322632100006 PM 23121110 ER PT J AU Celli, J Zahrt, TC AF Celli, Jean Zahrt, Thomas C. TI Mechanisms of Francisella tularensis Intracellular Pathogenesis SO COLD SPRING HARBOR PERSPECTIVES IN MEDICINE LA English DT Article ID LIVE VACCINE STRAIN; MURINE MACROPHAGES; REACTIVE NITROGEN; NADPH OXIDASE; IFN-GAMMA; SCHU S4; INTRAMACROPHAGE GROWTH; PHAGOSOMAL ESCAPE; RESPIRATORY BURST; INFLAMMASOME ACTIVATION AB Francisella tularensis is a zoonotic intracellular pathogen and the causative agent of the debilitating febrile illness tularemia. Although natural infections by F. tularensis are sporadic and generally localized, the low infectious dose, with the ability to be transmitted to humans via multiple routes and the potential to cause life-threatening infections, has led to concerns that this bacterium could be used as an agent of bioterror and released intentionally into the environment. Recent studies of F. tularensis and other closely related Francisella species have greatly increased our understanding of mechanisms used by this organism to infect and cause disease within the host. Here, we review the intracellular life cycle of Francisella and highlight key genetic determinants and/or pathways that contribute to the survival and proliferation of this bacterium within host cells. C1 [Celli, Jean] NIAID, Intracellular Parasites Lab, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. [Zahrt, Thomas C.] Med Coll Wisconsin, Ctr Infect Dis Res, Dept Microbiol & Mol Genet, Milwaukee, WI 53226 USA. RP Zahrt, TC (reprint author), Med Coll Wisconsin, Ctr Infect Dis Res, Dept Microbiol & Mol Genet, Milwaukee, WI 53226 USA. EM tzahrt@mcw.edu FU National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID) [1R21 AI097597-01A1]; NIAID's Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research Program; Region V Great Lakes Regional Center of Excellence (National Institutes of Health) [2-U54-AI-057153]; Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Diseases FX The authors apologize to those investigators in the field whose excellent work we were not able to reference. We are grateful to Anita Mora at the Rocky Mountain Laboratory for her assistance with graphic art. T.C.Z. acknowledges support from the National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIAID) (1R21 AI097597-01A1), and the NIAID's Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research Program. Membership within and support from the Region V Great Lakes Regional Center of Excellence (National Institutes of Health Award 2-U54-AI-057153) is also acknowledged. J.C. acknowledges support from the Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Diseases. NR 99 TC 34 Z9 35 U1 2 U2 17 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI COLD SPRING HARBOR PA 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA SN 2157-1422 J9 CSH PERSPECT MED JI Cold Spring Harb. Perspect. Med. PD APR PY 2013 VL 3 IS 4 AR a010314 DI 10.1101/cshperspect.a010314 PG 14 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 187ZK UT WOS:000322160300008 PM 23545572 ER PT J AU Moniuszko, M Lipinska, D Jeznach, M Kowal, K Grubczak, K Rusak, M McKinnon, K Vaccari, M Liyanage, NPM Fenizia, C Wawrusiewicz-Kurylonek, N Dabrowska, M Jablonska, E Kretowski, A Gorska, M Bodzenta-Lukaszyk, A AF Moniuszko, M. Lipinska, D. Jeznach, M. Kowal, K. Grubczak, K. Rusak, M. McKinnon, K. Vaccari, M. Liyanage, N. P. M. Fenizia, C. Wawrusiewicz-Kurylonek, N. Dabrowska, M. Jablonska, E. Kretowski, A. Gorska, M. Bodzenta-Lukaszyk, A. TI GLUCOCORTICOIDS UPREGULATE DECREASED IL-7 RECEPTOR EXPRESSION IN ASTHMATIC PATIENTS AND SIMIAN IMMUNODEFICIENCY VIRUS-INFECTED NON-HUMAN PRIMATES SO JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS LA English DT Article DE IL-7R (CD127); glucocorticoids; asthma; SIV infection; HIV infection ID CD8(+) T-CELLS; INTERLEUKIN-7 RECEPTOR; HIV-INFECTION; ANTIRETROVIRAL THERAPY; IL-7R-ALPHA EXPRESSION; IMMUNE ACTIVATION; CD4(+); CD127; INFLAMMATION; LYMPHOCYTES AB Signaling through interleukin-7 receptor (IL-7R) is essential for regulation of T-cell homeostasis and survival. Previously, we and others have found diminished IL-7R levels in simian immunodeficiency virus (SIV) - infected non-human primates and human immunodeficiency virus (HIV) - infected patients. To date, it remains unknown whether changes in IL-7R expression could also be linked to non-infectious inflammatory diseases such as asthma or anti-inflammatory drug use. Here, we investigated through flow cytometry the levels of IL-7R expression on CD4+ and CD4- T-cells in asthmatic patients in relation to disease severity, immune status and glucocorticoid (GC) use. In addition, we sought to evaluate the effects of in vivo and in vitro GC treatment on IL-7R expression in both asthmatic patients and SIV-infected non-human primates. We demonstrated that expression of IL-7R on peripheral blood CD4+ T-cells was significantly decreased in clinically stable GC-naive mild and moderate asthmatic patients. Accordingly, the development of asthmatic reaction following bronchial allergen challenge performed in sensitized subjects was associated with a significant drop in levels of IL-7R on circulating CD4+ T-cells. In contrast, CD4+ T-cells from both, mild and moderate, but not severe asthmatics, treated with inhaled GC displayed levels of IL-7R similar to that seen in healthy controls. We did not find significant differences with serum or sputum interleukin-7 levels among healthy controls and GC-naive and GC-treated asthmatic patients. Furthermore, both in vitro GC treatment and short-term oral GC administration to asthmatic patients resulted in a significant enhancement of IL-7R. Similarly, we demonstrated that GC-stimulated T-cells from SIV-infected non-human primates up-regulated IL-7R expression. Accordingly, experimental short-term systemic in vivo administration of GC to SIV-infected macaques led to enhancement of IL-7R expression on circulating T-cells. Our data indicate that GC bear potential to recover diminished IL-7R expression, as well in asthma as in lentiviral infection. C1 [Moniuszko, M.; Jeznach, M.; Kowal, K.; Bodzenta-Lukaszyk, A.] Med Univ Bialystok, Dept Allergol & Internal Med, PL-15276 Bialystok, Poland. [Moniuszko, M.] Med Univ Bialystok, Dept Regenerat Med & Immune Regulat, PL-15276 Bialystok, Poland. [Lipinska, D.; Wawrusiewicz-Kurylonek, N.; Kretowski, A.; Gorska, M.] Med Univ Bialystok, Dept Endocrinol Diabetol & Internal Med, PL-15276 Bialystok, Poland. [Grubczak, K.; Jablonska, E.] Med Univ Bialystok, Dept Immunol, PL-15276 Bialystok, Poland. [Rusak, M.; Dabrowska, M.] Med Univ Bialystok, Dept Hematol Diagnost, PL-15276 Bialystok, Poland. [McKinnon, K.] NCI, Vaccine Branch, FACS Core Facil, Bethesda, MD 20892 USA. [Vaccari, M.; Liyanage, N. P. M.; Fenizia, C.] NCI, Anim Models & Retroviral Vaccines Sect, Bethesda, MD 20892 USA. RP Moniuszko, M (reprint author), Med Univ Bialystok, Dept Allergol & Internal Med, Dept Regenerat Med & Immune Regulat, PL-15276 Bialystok, Poland. EM moniuszm@umb.edu.pl FU Polish National Science Center [NN401530240]; Medical University of Bialystok FX We thank Dr. Genoveffa Franchini (NCI, Bethesda, Md, USA) for generously allowing us the use of non-human primates in the study and helpful comments, discussions and critical evaluation of the manuscript. We also thank Dr Marjorie Robert-Guroff (NCI, Bethesda, Md, USA) for providing cells from SIV-infected macaques, Mrs. Teresa Michno for excellent laboratory assistance and Teresa Habina for editorial assistance. The work was supported by a grant from the Polish National Science Center (NN401530240) and grants from the Medical University of Bialystok. NR 30 TC 0 Z9 0 U1 0 U2 1 PU BIOLIFE SAS PI SILVA MARINA (TE) PA VIA S STEFANO 39 BIS, 64029 SILVA MARINA (TE), ITALY SN 0393-974X J9 J BIOL REG HOMEOS AG JI J. Biol. Regul. Homeost. Agents PD APR-JUN PY 2013 VL 27 IS 2 BP 427 EP 442 PG 16 WC Endocrinology & Metabolism; Immunology; Medicine, Research & Experimental; Physiology SC Endocrinology & Metabolism; Immunology; Research & Experimental Medicine; Physiology GA 189TH UT WOS:000322290700015 PM 23830393 ER PT J AU Batch, BC Ard, JD Vollmer, WM Funk, K Appel, LJ Stevens, VJ Samuel-Hodge, C Loria, CM Hollis, JF Svetkey, LP AF Batch, Bryan C. Ard, Jamy D. Vollmer, William M. Funk, Kristine Appel, Lawrence J. Stevens, Victor J. Samuel-Hodge, Carmen Loria, Catherine M. Hollis, Jack F. Svetkey, Laura P. TI Impact of Participant and Interventionist Race Concordance on Weight Loss Outcomes SO OBESITY LA English DT Article ID LOSS MAINTENANCE TRIAL; BLOOD-PRESSURE; CARDIOVASCULAR-DISEASE; NONPHARMACOLOGIC INTERVENTIONS; DIETARY-SODIUM; CLINICAL-TRIAL; PATIENT; HYPERTENSION; ADULTS; ACCELEROMETERS AB Objective: We have previously shown that racial composition of behavioral intervention groups does not affect achieved weight loss. However, it is unclear if the race of the interventionist affects intervention outcomes. The objective of this analysis is to estimate the impact of race concordance between participant and interventionist on weight change in the initial weight loss phase (phase I) of the Weight Loss Maintenance trial (WLM). Design and Methods: A total of 1,685 overweight or obese adults (BMI 25-45 kg/m(2)) who were taking medication for hypertension and/or dyslipidemia participated in phase I of the WLM trial. All participants received a 6-month intensive behavioral intervention in groups of 15-20 facilitated by a trained interventionist. The main outcome is change in weight at 6 months. Results: Participants were on average 55 years of age, 67% female and 44% African American (AA). Three of seventeen interventionists were AA, 14 were non-AA. Seventy-three percent of participants shared race concordance with the interventionist. There was a small but statistically significant difference in weight change of participants who were the same race as the interventionist (-5.84 kg, s.e. 0.17) as compared with those who were not race concordant (-5.04 kg, s.e. 0.33), a difference of 0.8 kg, (P = 0.04). The impact of concordance on weight change differed by race (i.e., interaction of race and concordance was significant, P = 0.02). Conclusions: In a post hoc analysis of a group-based behavioral intervention, race concordance for non-AA participants was associated with slightly greater weight loss. Race concordance was not associated with weight loss for AA participants. C1 [Batch, Bryan C.; Svetkey, Laura P.] Duke Univ, Med Ctr, Sarah W Stedman Nutr & Metab Ctr, Durham, NC 27710 USA. [Batch, Bryan C.] Duke Univ, Med Ctr, Dept Med, Div Endocrinol, Durham, NC 27710 USA. [Ard, Jamy D.] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA. [Ard, Jamy D.] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA. [Vollmer, William M.; Funk, Kristine; Stevens, Victor J.; Hollis, Jack F.] Ctr Hlth Res Kaiser Permanente Northwest, Hlth Sci Program, Portland, OR USA. [Appel, Lawrence J.] Johns Hopkins Med Inst, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD 21205 USA. [Samuel-Hodge, Carmen] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Nutr, Chapel Hill, NC USA. [Samuel-Hodge, Carmen] Univ N Carolina, Ctr Hlth Promot & Dis Prevent, Chapel Hill, NC USA. [Loria, Catherine M.] NHLBI, Bethesda, MD 20892 USA. [Svetkey, Laura P.] Duke Univ, Med Ctr, Duke Hypertens Ctr, Dept Med,Div Nephrol, Durham, NC 27710 USA. RP Batch, BC (reprint author), Duke Univ, Med Ctr, Sarah W Stedman Nutr & Metab Ctr, Durham, NC 27710 USA. EM bryan.batch@duke.edu FU National Heart, Lung, Blood Institute [5-U01 HL68734, 5-U01 HL68676, 5-U01 HL68790, 5-U01 HL68920, 5-HL68955] FX The Weight Loss Maintenance trial was sponsored by National Heart, Lung, Blood Institute grants 5-U01 HL68734, 5-U01 HL68676, 5-U01 HL68790, 5-U01 HL68920, and 5-HL68955. NR 36 TC 5 Z9 5 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 J9 OBESITY JI Obesity PD APR PY 2013 VL 21 IS 4 BP 712 EP 717 DI 10.1038/oby.2012.184 PG 6 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 187AM UT WOS:000322087800009 PM 23712973 ER PT J AU Fesinmeyer, MD North, KE Ritchie, MD Lim, U Franceschini, N Wilkens, LR Gross, MD Buzkova, P Glenn, K Quibrera, PM Fernandez-Rhodes, L Li, Q Fowke, JH Li, RL Carlson, CS Prentice, RL Kuller, LH Manson, JE Matise, TC Cole, SA Chen, CTL Howard, BV Kolonel, LN Henderson, BE Monroe, KR Crawford, DC Hindorff, LA Buyske, S Haiman, CA Le Marchand, L Peters, U AF Fesinmeyer, Megan D. North, Kari E. Ritchie, Marylyn D. Lim, Unhee Franceschini, Nora Wilkens, Lynne R. Gross, Myron D. Buzkova, Petra Glenn, Kimberly Quibrera, P. Miguel Fernandez-Rhodes, Lindsay Li, Qiong Fowke, Jay H. Li, Rongling Carlson, Christopher S. Prentice, Ross L. Kuller, Lewis H. Manson, JoAnn E. Matise, Tara C. Cole, Shelley A. Chen, Christina T. L. Howard, Barbara V. Kolonel, Laurence N. Henderson, Brian E. Monroe, Kristine R. Crawford, Dana C. Hindorff, Lucia A. Buyske, Steven Haiman, Christopher A. Le Marchand, Loic Peters, Ulrike TI Genetic Risk Factors for BMI and Obesity in an Ethnically Diverse Population: Results from the Population Architecture Using Genomics and Epidemiology (PAGE) Study SO OBESITY LA English DT Article ID BODY-MASS INDEX; WIDE ASSOCIATION; FTO GENE; CARDIOVASCULAR-DISEASE; AMERICAN-INDIANS; EXTREME OBESITY; ADULT OBESITY; EARLY-ONSET; FAT MASS; VARIANTS AB Objective: Several genome-wide association studies (GWAS) have demonstrated that common genetic variants contribute to obesity. However, studies of this complex trait have focused on ancestrally European populations, despite the high prevalence of obesity in some minority groups. Design and Methods: As part of the "Population Architecture using Genomics and Epidemiology (PAGE)" Consortium, we investigated the association between 13 GWAS-identified single-nucleotide polymorphisms (SNPs) and BMI and obesity in 69,775 subjects, including 6,149 American Indians, 15,415 African-Americans, 2,438 East Asians, 7,346 Hispanics, 604 Pacific Islanders, and 37,823 European Americans. For the BMI-increasing allele of each SNP, we calculated beta coefficients using linear regression (for BMI) and risk estimates using logistic regression (for obesity defined as BMI >= 30) followed by fixed-effects meta-analysis to combine results across PAGE sites. Analyses stratified by racial/ethnic group assumed an additive genetic model and were adjusted for age, sex, and current smoking. We defined "replicating SNPs" (in European Americans) and "generalizing SNPs" (in other racial/ethnic groups) as those associated with an allele frequency-specific increase in BMI. Results: By this definition, we replicated 9/13 SNP associations (5 out of 8 loci) in European Americans. We also generalized 8/13 SNP associations (5/8 loci) in East Asians, 7/13 (5/8 loci) in African Americans, 6/13 (4/8 loci) in Hispanics, 5/8 in Pacific Islanders (5/8 loci), and 5/9 (4/8 loci) in American Indians. Conclusion: Linkage disequilibrium patterns suggest that tagSNPs selected for European Americans may not adequately tag causal variants in other ancestry groups. Accordingly, fine-mapping in large samples is needed to comprehensively explore these loci in diverse populations. C1 [Fesinmeyer, Megan D.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Dept Biostat & Biomath, Seattle, WA 98104 USA. [North, Kari E.] Univ N Carolina, Sch Publ Hlth, Carolina Ctr Genome Sci, Chapel Hill, NC USA. [North, Kari E.; Franceschini, Nora; Quibrera, P. Miguel; Fernandez-Rhodes, Lindsay] Univ N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA. [Ritchie, Marylyn D.; Glenn, Kimberly; Li, Qiong; Crawford, Dana C.] Vanderbilt Univ, Med Ctr, Ctr Human Genet Res, Nashville, TN USA. [Lim, Unhee; Wilkens, Lynne R.; Kolonel, Laurence N.; Le Marchand, Loic] Univ Hawaii, Ctr Canc, Canc Epidemiol Program, Honolulu, HI 96822 USA. [Gross, Myron D.] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Buzkova, Petra] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Quibrera, P. Miguel] Univ N Carolina, Sch Med, Dept Med, Chapel Hill, NC USA. [Fowke, Jay H.] Vanderbilt Univ, Med Ctr, Vanderbilt Epidemiol Ctr, Nashville, TN USA. [Li, Rongling; Hindorff, Lucia A.] NHGRI, Off Populat Genom, NIH, Bethesda, MD 20892 USA. [Carlson, Christopher S.; Prentice, Ross L.; Chen, Christina T. L.; Peters, Ulrike] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Kuller, Lewis H.] Univ Pittsburgh, Dept Med, Pittsburgh, PA USA. [Manson, JoAnn E.] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Prevent Med,Dept Med, Boston, MA 02115 USA. [Manson, JoAnn E.] Harvard Univ, Brigham & Womens Hosp, Sch Publ Hlth, Sch Med,Dept Epidemiol, Boston, MA 02115 USA. [Matise, Tara C.; Buyske, Steven] Rutgers State Univ, Dept Stat & Biostat, Piscataway, NJ USA. [Cole, Shelley A.] SW Fdn Biomed Res, Dept Genet, San Antonio, TX USA. [Howard, Barbara V.] MedStar Hlth Res Inst, Hyattsville, MD USA. [Howard, Barbara V.] Georgetown Univ, Dept Med, Div Endocrinol & Metab, Washington, DC USA. [Henderson, Brian E.; Monroe, Kristine R.; Haiman, Christopher A.] Univ So Calif, Keck Sch Med, Kenneth Norris Jr Comprehens Canc Ctr, Dept Prevent Med, Los Angeles, CA 90033 USA. [Buyske, Steven] Rutgers State Univ, Dept Genet, Piscataway, NJ USA. RP Peters, U (reprint author), Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, 1124 Columbia St, Seattle, WA 98104 USA. EM upeters@fhcrc.org OI Glenn, Kimberly/0000-0002-6765-5592; Buyske, Steven/0000-0001-8539-5416 FU National Human Genome Research Institute (NHGRI); CAL-iCo [U01HG004803]; EAGLE [U01HG004798]; MEC [U01HG004802]; WHI [U01HG 004790]; Coordinating Center [U01HG004801]; NHGRI ARRA FX The Population Architecture Using Genomics and Epidemiology (PAGE) program is funded by the National Human Genome Research Institute (NHGRI), supported by U01HG004803 (CAL-iCo), U01HG004798 (EAGLE), U01HG004802 (MEC), U01HG 004790 (WHI), and U01HG004801 (Coordinating Center), and their respective NHGRI ARRA supplements. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The complete list of PAGE members can be found at http://www.pagestudy.org. NR 39 TC 21 Z9 21 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 J9 OBESITY JI Obesity PD APR PY 2013 VL 21 IS 4 BP 835 EP 846 DI 10.1038/oby.2012.158 PG 12 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 187AM UT WOS:000322087800026 PM 23712987 ER PT J AU Kim, HS Headey, SJ Yoga, YMK Scanlon, MJ Gorospe, M Wilce, MCJ Wilce, JA AF Kim, Henry S. Headey, Stephen J. Yoga, Yano M. K. Scanlon, Martin J. Gorospe, Myriam Wilce, Matthew C. J. Wilce, Jacqueline A. TI Distinct binding properties of TIAR RRMs and linker region SO RNA BIOLOGY LA English DT Article DE RNA-binding protein; TIAR; TIA-1; RRM; C-terminal extension; translational regulation; surface plasmon resonance (SPR); NMR ID RNA RECOGNITION MOTIFS; AU-RICH ELEMENTS; INTRACELLULAR ANTIGEN-1 TIA-1; MAMMALIAN STRESS GRANULES; PRE-MESSENGER-RNA; 5'-SPLICE SITES; PROTEIN TIAR; SPLICING REGULATION; GENE-EXPRESSION; HUR AB The RNA-binding protein TIAR is an mRNA-binding protein that acts as a translational repressor, particularly important under conditions of cellular stress. It binds to target mRNA and DNA via its RNA recognition motif (RRM) domains and is involved in both splicing regulation and translational repression via the formation of stress granules. TIAR has also been shown to bind ssDNA and play a role in the regulation of transcription. Here we show, using surface plasmon resonance and nuclear magnetic resonance spectroscopy, specific roles of individual TIAR domains for high-affinity binding to RNA and DNA targets. We confirm that RRM2 of TIAR is the major RNA- and DNA-binding domain. However, the strong nanomolar affinity binding to U-rich RNA and T-rich DNA depends on the presence of the six amino acid residues found in the linker region C-terminal to RRM2. On its own, RRM1 shows preferred binding to DNA over RNA. We further characterize the interaction between RRM2 with the C-terminal extension and an AU-rich target RNA sequence using NMR spectroscopy to identify the amino acid residues involved in binding. We demonstrate that TIAR RRM2, together with its C-terminal extension, is the major contributor for the high-affinity (nM) interactions of TIAR with target RNA sequences. C1 [Kim, Henry S.; Yoga, Yano M. K.; Wilce, Matthew C. J.; Wilce, Jacqueline A.] Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3004, Australia. [Headey, Stephen J.; Scanlon, Martin J.] Monash Univ, Monash Inst Pharmaceut Sci, Melbourne, Vic 3004, Australia. [Scanlon, Martin J.] Monash Univ, ARC Ctr Excellence Coherent Xray Sci, Melbourne, Vic 3004, Australia. [Gorospe, Myriam] NIA, Genet Lab, Intramural Res Program, NIH, Baltimore, MD 21224 USA. RP Wilce, MCJ (reprint author), Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3004, Australia. EM matthew.wilce@monash.edu; jackie.wilce@monash.edu OI Wilce, Jacqueline/0000-0002-8344-2626; Scanlon, Martin/0000-0002-9230-7506 FU Australian Research Council [DP110102056]; National Institute on Aging-Intramural Research Program, National Institutes of Health FX lThis work was supported by the Australian Research Council (DP110102056 awarded to M.C.J.W., J.A.W. and M.G.). M.C.J.W. is a fellow of the National Health and Medical Research Council of Australia. M.G. was supported by the National Institute on Aging-Intramural Research Program, National Institutes of Health. NR 50 TC 8 Z9 8 U1 0 U2 2 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1547-6286 J9 RNA BIOL JI RNA Biol. PD APR 1 PY 2013 VL 10 IS 4 BP 579 EP 589 DI 10.4161/rna.24341 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 181WU UT WOS:000321701600013 PM 23603827 ER PT J AU Larochelle, A Dunbar, CE AF Larochelle, Andre Dunbar, Cynthia E. TI Hematopoietic Stem Cell Gene Therapy: Assessing the Relevance of Preclinical Models SO SEMINARS IN HEMATOLOGY LA English DT Article ID CHRONIC GRANULOMATOUS-DISEASE; WISKOTT-ALDRICH-SYNDROME; SEVERE COMBINED IMMUNODEFICIENCY; BONE-MARROW-CELLS; HUMAN ADENOSINE-DEAMINASE; BLOOD CD34(+) CELLS; LONG-TERM ENGRAFTMENT; RETROVIRAL-MEDIATED TRANSFER; MURINE BETA-THALASSEMIA; EX-VIVO EXPANSION AB The modern laboratory mouse has become a central tool of for biomedical research with a notable influence in the field of hematopoiesis. Application of retroviral-based gene transfer approaches to mouse hematopoietic stem cells (HSCs) has led to a sophisticated understanding of the hematopoietic hierarchy in this model. However, the assumption that gene transfer methodologies developed in the mouse could be similarly applied to human HSCs for the treatment of human diseases left the field of gene therapy in a decade-long quandary. It is not until more relevant humanized xenograft mouse models and phylogenetically related large animal species were used to optimize gene transfer methodologies that unequivocal clinical successes were achieved. However, the subsequent reporting of severe adverse events in these clinical trials cast doubts on the predictive value of conventional preclinical testing, and encouraged the development of new assays for assessing the relative genotoxicity of various vector designs. C1 [Larochelle, Andre; Dunbar, Cynthia E.] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA. RP Larochelle, A (reprint author), NHLBI, NIH, Bldg 10CRC,Room 3-5256,9000 Rockville Pike, Bethesda, MD 20892 USA. EM larochea@nhlbi.nih.gov FU Intramural NIH HHS [Z99 HL999999] NR 306 TC 3 Z9 3 U1 0 U2 8 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 2013 VL 50 IS 2 BP 101 EP 130 DI 10.1053/j.seminhematol.2013.03.025 PG 30 WC Hematology SC Hematology GA 174NZ UT WOS:000321164000004 PM 24014892 ER PT J AU Scheinberg, P Chen, JC AF Scheinberg, Phillip Chen, Jichun TI Aplastic Anemia: What Have We Learned From Animal Models and From the Clinic SO SEMINARS IN HEMATOLOGY LA English DT Article ID BONE-MARROW FAILURE; PAROXYSMAL-NOCTURNAL HEMOGLOBINURIA; HEMATOPOIETIC STEM-CELLS; REGULATORY T-CELLS; BENZENE-INDUCED HEMATOTOXICITY; HIGH-DOSE CYCLOPHOSPHAMIDE; MOUSE MODEL; DYSKERATOSIS-CONGENITA; PIGA GENE; CHLORAMPHENICOL SUCCINATE AB Aplastic anemia (AA) is currently perceived as an immune-mediated disease in which aberrant effector cells recognize and destroy primitive marrow elements, resulting in pancytopenia. The immune hypothesis is based on clinical observations of responsiveness of AA to immunomodulatory agents such as anti-thymocyte globulin (ATG) and the requirement of cyclosporine to maintain response; evidence of an immune system in disarray provided by abnormal regulatory, TH1, TH17, and expanded CD8(+) T-cell populations, and animal models of immune-mediated marrow destruction, where many of the observed clinical and in vitro alterations can be confirmed and expanded. Murine models mimicking AA have used exposure to agents that result in marrow destruction through a direct toxic effect, but models that explore antigenic disparities between strains have resulted in immune-mediated destruction of the marrow, more closely modeling human AA. Many experiments in mice have helped confirm and elucidate specific mechanisms of marrow destruction. However, clinical development of regimens in AA has not relied on establishing their success in murine model. Instead, drugs and their combinations investigated in AA were those shown clinically to be active in AA, in other hematologic diseases, or in other specialties such as in rheumatology, and solid and bone marrow transplantation. In this review, the evolution of murine models and their clinical relevance in AA are discussed. Semin Hematol 50:156-164. (C) 2013 Published by Elsevier Inc. C1 [Scheinberg, Phillip; Chen, Jichun] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA. RP Scheinberg, P (reprint author), 10 Ctr Dr,Bldg 10 CRC,Room 3E-5140,MSC 1202, Bethesda, MD 20892 USA. EM scheinbp@mail.nih.gov OI Scheinberg, Phillip/0000-0002-9047-4538 FU NIH, National Heart, Lung, and Blood Institute FX The authors would like to thank Neal S. Young for his help in preparing the manuscript. This research was supported by the Intramural Research Program of the NIH, National Heart, Lung, and Blood Institute. NR 103 TC 7 Z9 9 U1 2 U2 14 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 2013 VL 50 IS 2 BP 156 EP 164 DI 10.1053/j.seminhematol.2013.03.028 PG 9 WC Hematology SC Hematology GA 174NZ UT WOS:000321164000007 PM 24216172 ER PT J AU Calado, RT Dumitriu, B AF Calado, Rodrigo T. Dumitriu, Bogdan TI Telomere Dynamics in Mice and Humans SO SEMINARS IN HEMATOLOGY LA English DT Article ID LINKED DYSKERATOSIS-CONGENITA; DIAMOND-BLACKFAN ANEMIA; TERT PROMOTER MUTATIONS; CORONARY-HEART-DISEASE; ACUTE MYELOID-LEUKEMIA; REVERSE-TRANSCRIPTASE; PULMONARY-FIBROSIS; APLASTIC-ANEMIA; CHROMOSOMAL INSTABILITY; MAMMALIAN TELOMERES AB Telomeres are ribonucleoprotein structures capping the end of every linear chromosome. In all vertebrates, they are composed of TTAGGG repeats coated with specific protecting proteins. Telomeres shorten with each mitotic cell division, but telomerase, a reverse transcriptase, elongate telomeres in very specific cells, such as embryonic and adult stem cells. Although telomere sequence is identical in mice and humans and telomeres serve the same role of protecting chromosomes and genetic information from damage and erosion in both species, abnormalities in telomere maintenance and in telomerase function do not coincide in phenotype in humans and mice. The telomeres of most laboratory mice are 5 to 10 times longer than in humans, but their lifespan is 30 times shorter. Complete absence of telomerase has little expression in phenotype over several generations in mice, whereas heterozygosity for telomerase mutations in humans is sufficient to result in organ regeneration defect and cancer development. Patients with telomerase deficiency and very short telomeres may develop aplastic anemia, pulmonary fibrosis, or cirrhosis, whereas telomerase-null murine models display only modest hematopoietic deficiency and develop emphysema when exposed to cigarette smoke. In summary, telomerase deficiency in both humans and mice accelerate telomere shortening, but its consequences in the different organs and in the organism diverge, mainly due to telomere length differences. Semin Hematol 50:165-174. (C) 2013 Elsevier Inc. All rights reserved. C1 [Calado, Rodrigo T.] Univ Sao Paulo, Dept Internal Med, Sch Med, BR-14049900 Ribeirao Preto, SP, Brazil. [Dumitriu, Bogdan] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA. RP Calado, RT (reprint author), Univ Sao Paulo, Dept Internal Med, Sch Med, HCRP Lab Hematol, Av Bandeirantes,3900,Bloco G, BR-14049900 Ribeirao Preto, SP, Brazil. EM rtcalado@fmrp.usp.br RI Calado, Rodrigo/G-2619-2011 FU FAPESP; NIH FX Supported in part by FAPESP (R.T.C.) and the NIH Intramural Research Program (B.D.). NR 90 TC 25 Z9 25 U1 0 U2 13 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 2013 VL 50 IS 2 BP 165 EP 174 DI 10.1053/j.seminhematol.2013.03.030 PG 10 WC Hematology SC Hematology GA 174NZ UT WOS:000321164000008 PM 23956466 ER PT J AU Lozier, JN Nichols, TC AF Lozier, Jay N. Nichols, Timothy C. TI Animal Models of Hemophilia and Related Bleeding Disorders SO SEMINARS IN HEMATOLOGY LA English DT Article ID VON-WILLEBRAND-FACTOR; FACTOR-VIII GENE; COAGULATION-FACTOR-IX; BONE-MARROW-TRANSPLANTATION; IN-VIVO CHARACTERIZATION; RECOMBINANT FACTOR-VIII; DEFICIENT MOUSE MODEL; WEIBEL-PALADE BODIES; LONG-TERM CORRECTION; OPEN-LABEL TRIAL AB Animal models of hemophilia and related diseases are important for the development of novel treatments and to understand the pathophysiology of bleeding disorders in humans. Testing in animals with the equivalent human disorder provides informed estimates of doses and measures of efficacy, which aids in design of human trials. Many models of hemophilia A, hemophilia B, and von Willebrand disease (VWD) have been developed from animals with spontaneous mutations (hemophilia A dogs, rats, sheep; hemophilia B dogs; and VWD pigs and dogs), or by targeted gene disruption in mice to create hemophilia A, B, or VWD models. Animal models have been used to generate new insights into the pathophysiology of each bleeding disorder and also to perform preclinical assessments of standard protein replacement therapies, as well as novel gene transfer technology. The differences both between species and in underlying causative mutations must be considered in choosing the best animal for a specific scientific study. Semin Hematol 50:175-184. Published by Elsevier Inc. C1 [Lozier, Jay N.] NIH, Ctr Clin, Dept Lab Med, Bethesda, MD 20892 USA. [Nichols, Timothy C.] Univ N Carolina, Dept Med, Chapel Hill, NC USA. [Nichols, Timothy C.] Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC USA. RP Lozier, JN (reprint author), NIH, Ctr Clin, Dept Lab Med, Bldg 10,Room 2C306,MSC 1508, Bethesda, MD 20892 USA. EM lozierjn@cc.nih.gov FU National Hemophilia Foundation; NIH Intramural Research Program [R24 HL63098] FX Supported in part by a Career Development Award from the National Hemophilia Foundation and the NIH Intramural Research Program (J.N.L.) and R24 HL63098 (T.C.N.). NR 93 TC 9 Z9 9 U1 1 U2 15 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 2013 VL 50 IS 2 BP 175 EP 184 DI 10.1053/j.seminhematol.2013.03.023 PG 10 WC Hematology SC Hematology GA 174NZ UT WOS:000321164000009 PM 23956467 ER PT J AU Wald, I Degnan, KA Gorodetsky, E Charney, DS Fox, NA Fruchter, E Goldman, D Lubin, G Pine, DS Bar-Haim, Y AF Wald, Ilan Degnan, Kathryn A. Gorodetsky, Elena Charney, Dennis S. Fox, Nathan A. Fruchter, Eyal Goldman, David Lubin, Gad Pine, Daniel S. Bar-Haim, Yair TI Attention to Threats and Combat-Related Posttraumatic Stress Symptoms Prospective Associations and Moderation by the Serotonin Transporter Gene SO JAMA PSYCHIATRY LA English DT Article ID BIASED ATTENTION; PROMOTER REGION; DISORDER; ANXIETY; POLYMORPHISM; PTSD; STIMULI; IRAQ; SUPPRESSION; PLASTICITY AB Importance: Combat places soldiers at risk for post-traumatic stress disorder (PTSD). The excessive rates of PTSD and other adjustment disorders in soldiers returning home make it imperative to identify risk and resilience factors that could be targeted by novel therapeutic treatments. Objective: To investigate the interplay among attention to threat, combat exposure, and other risk factors for PTSD symptoms in soldiers deployed to combat. Design and Setting: Longitudinal prospective study of Israeli Defense Force infantry soldiers carried out in 2008 through 2010. Repeated measurements during a 1-year period included baseline and predeployment data collected in training camps and deployment data collected in the combat theater. Participants: Infantry soldiers (1085 men; mean age, 18.8 years). Main Outcome Measures: Postcombat PTSD symptoms. Results: Soldiers developed threat vigilance during combat deployment, particularly when they were exposed to high-intensity combat, as indicated by faster response times to targets appearing at the location of threat relative to neutral stimuli (P < .001). Threat-related attention bias also interacted with combat exposure to predict risk for PTSD (P < .05). Bias toward threat at recruitment (P < .001) and bias away from threat just before deployment (P < .05) predicted postcombat PTSD symptoms. Moreover, these threat-related attention associations with PTSD were moderated by genetic and environmental factors, including serotonin transporter (5-HTTLPR) genotype. Conclusions and Relevance: Combat exposure interacts with threat-related attention to place soldiers at risk for PTSD, and interactions with other risk factors account for considerable variance in PTSD vulnerability. Understanding these associations informs research on novel attention bias modification techniques and prevention of PTSD. C1 [Wald, Ilan; Bar-Haim, Yair] Tel Aviv Univ, Sch Psychol Sci, IL-69978 Tel Aviv, Israel. [Wald, Ilan; Bar-Haim, Yair] Tel Aviv Univ, Sch Neurosci, IL-69978 Tel Aviv, Israel. [Degnan, Kathryn A.; Fox, Nathan A.] Univ Maryland, Dept Human Dev, College Pk, MD 20742 USA. [Gorodetsky, Elena] NIMH, Mood & Anxiety Disorders Program, Intramural Res Program, Bethesda, MD 20892 USA. [Pine, Daniel S.] NIMH, Sect Dev & Affect Neurosci, Emot & Dev Branch, Intramural Res Program, Bethesda, MD 20892 USA. [Charney, Dennis S.] Mt Sinai Sch Med, Dept Psychiat, New York, NY USA. [Charney, Dennis S.] Mt Sinai Sch Med, Dept Neurosci, New York, NY USA. [Charney, Dennis S.] Mt Sinai Sch Med, Dept Pharmacol & Syst Therapeut, New York, NY USA. [Fruchter, Eyal; Lubin, Gad] IDF Med Corps, Dept Mental Hlth, Tel Hashomer, Israel. [Goldman, David] NIAAA, Sect Human Neurogenet, NIH, Bethesda, MD USA. RP Bar-Haim, Y (reprint author), Tel Aviv Univ, Sch Psychol Sci, IL-69978 Tel Aviv, Israel. EM yair1@post.tau.ac.il RI Goldman, David/F-9772-2010 OI Goldman, David/0000-0002-1724-5405 FU Leo and Julia Forchheimer Foundation; National Institute of Mental Health; Mount Sinai School of Medicine FX This work was supported in part by The Leo and Julia Forchheimer Foundation in collaboration with the Mount Sinai School of Medicine and in part by the Intramural Research Program of the National Institute of Mental Health NR 41 TC 40 Z9 40 U1 4 U2 30 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-622X J9 JAMA PSYCHIAT JI JAMA Psychiatry PD APR PY 2013 VL 70 IS 4 BP 401 EP 409 DI 10.1001/2013.jamapsychiatry.188 PG 9 WC Psychiatry SC Psychiatry GA 170LW UT WOS:000320853400006 PM 23407816 ER PT J AU Hay, SI George, DB Moyes, CL Brownstein, JS AF Hay, Simon I. George, Dylan B. Moyes, Catherine L. Brownstein, John S. TI Big Data Opportunities for Global Infectious Disease Surveillance SO PLOS MEDICINE LA English DT Editorial Material ID HEALTH SURVEILLANCE; DENGUE; WEB; VECTORS; ECOLOGY; TRENDS; MODEL C1 [Hay, Simon I.; Moyes, Catherine L.] Univ Oxford, Dept Zool, Spatial Ecol & Epidemiol Grp, Oxford OX1 3PS, England. [Hay, Simon I.; George, Dylan B.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Brownstein, John S.] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA. [Brownstein, John S.] Boston Childrens Hosp, Childrens Hosp Informat Program, Boston, MA USA. RP Hay, SI (reprint author), Univ Oxford, Dept Zool, Spatial Ecol & Epidemiol Grp, S Parks Rd, Oxford OX1 3PS, England. EM simon.hay@zoo.ox.ac.uk RI Chiang, Vincent, Ming-Hsien/D-4312-2016; Hay, Simon/F-8967-2015; OI Chiang, Vincent, Ming-Hsien/0000-0002-2029-7863; Hay, Simon/0000-0002-0611-7272; Moyes, Catherine/0000-0002-8028-4079 FU NLM NIH HHS [G08 LM009776, R01 LM010812]; Wellcome Trust [091835, 095066] NR 33 TC 41 Z9 44 U1 8 U2 103 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1549-1676 J9 PLOS MED JI PLos Med. PD APR PY 2013 VL 10 IS 4 AR UNSP e1001413 DI 10.1371/journal.pmed.1001413 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA 154JA UT WOS:000319669600003 PM 23565065 ER PT J AU Kouwenhoven, A Minassian, VD Marsh, JW AF Kouwenhoven, Arlette Minassian, Violette Der Marsh, Jon W. TI HIV-1 Nef Mediates Pak Phosphorylation of Mek1 Serine298 and Elicits an Active Phospho-state of Pak2 SO CURRENT HIV RESEARCH LA English DT Article DE Nef; Pak; Mek; kinase; T cell; phosphorylation ID IMMUNODEFICIENCY-VIRUS TYPE-1; T-CELL-ACTIVATION; SMALL-MOLECULE INHIBITOR; NF-KAPPA-B; CROSS-CASCADE ACTIVATION; P21-ACTIVATED KINASE 2; LONG TERMINAL REPEAT; REGULATED KINASE; DOWN-REGULATION; GAMMA-PAK AB The HIV-1 Nef protein brings about increased T cell activity and viral titers through mechanisms that are poorly understood. Nef activity has been described as an enhancer, but not an inducer, of certain signaling pathways that lead to T cell activation and viral production, particularly from resting T cells. The protein has also been found to associate with and promote autophosphorylation of a serine kinase, Pak2, but the Nef-associated kinase level is very low and difficult to study. Here we demonstrate that Nef expression mediates phosphorylation of Mek1 serine298 in T cell lines as well as primary human T cells, thus directly affecting the Erk cascade. This phosphorylation is through a Pak and Rac activity. We also find that Pak2 in Nef expressing cells is phosphorylated on serine192/197, the first biochemical description of the Nef-mediated activation state for this kinase. C1 [Kouwenhoven, Arlette; Minassian, Violette Der; Marsh, Jon W.] NIMH, Lab Cellular & Mol Regulat, Sect Directed Gene Transfer, Bethesda, MD 20892 USA. RP Marsh, JW (reprint author), NIMH, Bldg 49,Room 5A72,49 Convent Dr,MSC 4483, Bethesda, MD 20892 USA. EM marshj@mail.nih.gov FU Intramural Research Program of the NIMH/NIH FX We thank Michael Nunn and Klaus Strebel for their comments and criticisms. We thank Lee Eiden for the VB cells; the NIH AIDS Research & Reference Reagent Program for providing H9 cells (Robert Gallo); and the Blood Services Section, Department of Transfusion Medicine, NIH, for providing elutriated lymphocytes. The Intramural Research Program of the NIMH/NIH supported this research. NR 103 TC 2 Z9 2 U1 1 U2 4 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1570-162X J9 CURR HIV RES JI Curr. HIV Res. PD APR PY 2013 VL 11 IS 3 BP 198 EP 209 PG 12 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA 171HT UT WOS:000320916800004 PM 23746211 ER PT J AU Merk, A Subramaniam, S AF Merk, Alan Subramaniam, Sriram TI HIV-1 envelope glycoprotein structure SO CURRENT OPINION IN STRUCTURAL BIOLOGY LA English DT Article ID VIRUS TYPE-1 GP41; CRYOELECTRON TOMOGRAPHY; MEMBRANE-FUSION; NEUTRALIZING ANTIBODIES; GP120 CORE; INTERHELICAL INTERACTIONS; POTENT NEUTRALIZATION; FUNCTIONAL-ANALYSIS; ATOMIC-STRUCTURE; RATIONAL DESIGN AB The trimeric envelope glycoprotein of HIV-1, composed of gp120 and gp41 subunits, remains a major target for vaccine development. The structures of the core regions of monomeric gp120 and gp41 have been determined previously by X-ray crystallography. New insights into the structure of trimeric HIV-1 envelope glycoproteins are now coming from cryo-electron tomographic studies of the gp120/gp41 trimer as displayed on intact viruses and from cryo-electron microscopic studies of purified, soluble versions of the ectodomain of the trimer. Here, we review recent developments in these fields as they relate to our understanding of the structure and function of HIV-1 envelope glycoproteins. C1 [Merk, Alan; Subramaniam, Sriram] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Subramaniam, S (reprint author), NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA. EM ss1@nih.gov FU National Cancer Institute, NIH, Bethesda, MD FX This work was supported by funds from the intramural program of the National Cancer Institute, NIH, Bethesda, MD. We thank Tom Goddard and Elaine Meng for advice with the use of UCSF Chimera for figure preparation, and Jacqueline Milne, Erin Tran, Lesley Earl, Joel Meyerson and Alberto Bartesaghi for helpful comments on the manuscript. NR 82 TC 29 Z9 29 U1 2 U2 48 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0959-440X J9 CURR OPIN STRUC BIOL JI Curr. Opin. Struct. Biol. PD APR PY 2013 VL 23 IS 2 BP 268 EP 276 DI 10.1016/j.sbi.2013.03.007 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 169AE UT WOS:000320748500016 PM 23602427 ER PT J AU Irwin, DJ Abrams, JY Schonberger, LB Leschek, EW Mills, JL Lee, VMY Trojanowski, JQ AF Irwin, David J. Abrams, Joseph Y. Schonberger, Lawrence B. Leschek, Ellen Werber Mills, James L. Lee, Virginia M. -Y. Trojanowski, John Q. TI Evaluation of Potential Infectivity of Alzheimer and Parkinson Disease Proteins in Recipients of Cadaver-Derived Human Growth Hormone SO JAMA NEUROLOGY LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; CEREBRAL BETA-AMYLOIDOSIS; ALPHA-SYNUCLEIN; NEURODEGENERATIVE DISEASES; SPONGIFORM ENCEPHALOPATHY; GRAFTED NEURONS; LONG-TERM; TRANSMISSION; PATHOLOGY; PITUITARY AB Importance: Growing evidence of cell-to-cell transmission of neurodegenerative disease (ND)-associated proteins (NDAPs) (ie, tau, A beta, and alpha-synuclein) suggests possible similarities to the infectious prion protein (PrPsc) in spongiform encephalopathies. There are limited data on the potential human-to-human transmission of NDAPs associated with Alzheimer disease (AD) and other non-PrPsc ND. Objective: To examine evidence for human-to-human transmission of AD, Parkinson disease (PD), and related NDAPs in cadaveric human growth hormone (c-hGH) recipients. Design: We conducted a detailed immunohistochemical analysis of pathological NDAPs other than PrPsc in human pituitary glands. We also searched for ND in recipients of pituitary-derived c-hGH by reviewing the National Hormone and Pituitary Program (NHPP) cohort database and medical literature. Setting: University-based academic center and agencies of the US Department of Health and Human Services. Participants: Thirty-four routine autopsy subjects (10 non-ND controls and 24 patients with ND) and a US cohort of c-hGH recipients in the NHPP. Main Outcome Measures: Detectable NDAPs in human pituitary sections and death certificate reports of non-PrPsc ND in the NHPP database. Results: We found mild amounts of pathological tau, A beta, and alpha-synuclein deposits in the adeno/neurohypophysis of patients with ND and control patients. No cases of AD or PD were identified, and 3 deaths attributed to amyotrophic lateral sclerosis (ALS) were found among US NHPP c-hGH recipients, including 2 of the 796 decedents in the originally confirmed NHPP c-hGH cohort database. Conclusions and Relevance: Despite the likely frequent exposure of c-hGH recipients to NDAPs, and their markedly elevated risk of PrPsc-related disease, this population of NHPP c-hGH recipients does not appear to be at increased risk of AD or PD. We discovered 3 ALS cases of unclear significance among US c-hGH recipients despite the absence of pathological deposits of ALS-associated proteins (TDP-43, FUS, and ubiquilin) in human pituitary glands. In this unique in vivo model of human-to-human transmission, we found no evidence to support concerns that NDAPs underlying AD and PD transmit disease in humans despite evidence of their cell-to-cell transmission in model systems of these disorders. Further monitoring is required to confirm these conclusions. C1 [Irwin, David J.; Lee, Virginia M. -Y.; Trojanowski, John Q.] Univ Penn, Perelman Sch Med, Ctr Neurodegenerat Dis Res, Dept Pathol & Lab Med,Inst Aging, Philadelphia, PA 19104 USA. [Irwin, David J.] Univ Penn, Perelman Sch Med, Dept Neurol, Philadelphia, PA 19104 USA. [Leschek, Ellen Werber] NIDDKD, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Mills, James L.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Dept Hlth & Human Serv, Bethesda, MD USA. [Abrams, Joseph Y.; Schonberger, Lawrence B.] Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA. RP Trojanowski, JQ (reprint author), Univ Penn, Sch Med, Ctr Neurodegenerat Dis Res, Maloney 3rd Floor,36th & Spruce St, Philadelphia, PA 19104 USA. EM trojanow@mail.med.upenn.edu FU AstraZeneca; Bristol-Myers Squibb; AD Core Centre grant; National Institute on Aging and Intramural Research Program [T32-AG000255]; National Institute of Child Health and Development, National Institutes of Health; [P30 AG010124-20] FX The authors report no financial disclosures related to the current study. Drs Lee and Trojanowski report single consulting services to Pfizer, Johnson & Johnson, MetLife, and Bristol-Myers Squibb; royalty payments through Penn licenses; and research support from AstraZeneca and Bristol-Myers Squibb.; This study was supported by grant P30 AG010124-20, an AD Core Centre grant, and grant T32-AG000255 from the National Institute on Aging and Intramural Research Program and the National Institute of Child Health and Development, National Institutes of Health. NR 46 TC 62 Z9 63 U1 1 U2 21 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-6149 J9 JAMA NEUROL JI JAMA Neurol. PD APR PY 2013 VL 70 IS 4 BP 462 EP 468 DI 10.1001/jamaneurol.2013.1933 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA 160NS UT WOS:000320127000006 PM 23380910 ER PT J AU Douglass, D Islam, N Baranowski, J Chen, TA Subar, AF Zimmerman, TP Baranowski, T AF Douglass, Deirdre Islam, Noemi Baranowski, Janice Chen, Tzu-An Subar, Amy F. Zimmerman, Thea Palmer Baranowski, Tom TI Simulated Adaptations to an Adult Dietary Self-Report Tool to Accommodate Children: Impact on Nutrient Estimates SO JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION LA English DT Article DE ASA24; children; dietary recall; self-administered; Automated Multiple Pass Method (AMPM); Automated Self-Administered 24-Hour Recall (ASA24); ASA24-Kids; Food and Nutrient Database for Dietary Studies (FNDDS) ID RECALL; ACCURACY; SYSTEM AB Objective: To simulate the effect of child-friendly (CF) adaptations of the National Cancer Institute's Automated Self-Administered 24-Hour Dietary Recall (ASA24) on estimates of nutrient intake. Method: One hundred twenty children, 8-13years old, entered their previous day's intake using the ASA24 and completed an interviewer-administered recall using the Nutrition Data System for Research (NDSR). Based on a hypothesis that proposed adaptations to the ASA24 will not significantly affect mean nutrient estimates, ASA24 data were manipulated postadministration to simulate a CF version in which 2 categories of data collection were removed: (1) foods not likely to be consumed by children (45%) based on previous analyses of national dietary data and (2) food detail questions (probes) to which children are unlikely to know the answers (46%), based on our experience. Results: Mean estimates of select nutrients between the beta version of ASA24 and the simulated CF recall showed no significant differences, indicating that the food and probe elimination did not significantly affect results. However, a comparison of total sugar and vitamin C assessments between the original ASA24, the CF version, and NDSR showed that the daily nutrient totals for both nutrients were significantly higher in the self-administered methods (both ASA24 and CF version) than in NDSR (interviewer-administered), which warrants a review of different methods for obtaining information about foods that are sources of these nutrients. Conclusion: The simulation of CF adaptations showed that it is feasible to implement, thereby reducing CF response burden without significantly affecting the results. C1 [Douglass, Deirdre; Islam, Noemi; Baranowski, Janice; Chen, Tzu-An; Baranowski, Tom] Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Subar, Amy F.] NCI, Div Canc Control & Populat Sci, Appl Res Program, Risk Factor Monitoring & Methods Branch, Bethesda, MD 20892 USA. [Zimmerman, Thea Palmer] WESTAT Corp, Rockville, MD 20850 USA. RP Douglass, D (reprint author), Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr, 1100 Bates St,Rm 6064, Houston, TX 77030 USA. EM d.douglass@earthlink.net OI Baranowski, Tom/0000-0002-0653-2222 FU National Cancer Institute [5 U01 CA130762]; U.S. Department of Agriculture, Agricultural Research Service [58-6250-6001] FX This research was funded by a grant from the National Cancer Institute (5 U01 CA130762) and institutional support from the U.S. Department of Agriculture, Agricultural Research Service (Cooperative Agreement No. 58-6250-6001). NR 17 TC 5 Z9 5 U1 0 U2 5 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0731-5724 J9 J AM COLL NUTR JI J. Am. Coll. Nutr. PD APR 1 PY 2013 VL 32 IS 2 BP 92 EP 97 DI 10.1080/07315724.2013.789339 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 167AH UT WOS:000320602000003 PM 24015716 ER PT J AU Byun, JS Gardner, K AF Byun, Jung S. Gardner, Kevin TI Wounds That Will Not Heal Pervasive Cellular Reprogramming in Cancer SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Review ID EPITHELIAL-MESENCHYMAL TRANSITION; TUMOR-ASSOCIATED MACROPHAGES; HUMAN BREAST-CANCER; E-CADHERIN EXPRESSION; MUSCLE ACTIN EXPRESSION; STEM-CELLS; GRANULATION-TISSUE; MYOFIBROBLAST DIFFERENTIATION; REPRODUCTIVE FACTORS; HUMAN FIBROBLASTS AB There has been an explosion of articles on epithelial-mesenchymal transition and other modes of cellular reprogramming that influence the tumor microenvironment. Many controversies exist and remain to be resolved. The interest of the pathologists in the molecular and functional parallels between wound healing and the developing tumor stroma has its earliest origin in the writings of Rudolph Virchow in the 19th century. Since then, most of the focus has been primarily on the dynamics of the extracellular matrix; however, new interest has been redirected toward deciphering and understanding the enigmatic, yet elegant, plasticity of the cellular components of the proliferating epithelia and stroma and how they are reciprocally influenced. Citing several examples from breast cancer research, we will trace how these perspectives have unfolded in the pages of The American Journal of Pathology and other investigative journals during the past century, their impact, and where the field is headed. C1 [Byun, Jung S.; Gardner, Kevin] NCI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. RP Gardner, K (reprint author), NCI, Genet Branch, Dept Hlth & Human Services, NIH,Ctr Canc Res, Bldg 41,Room D305, Bethesda, MD 20892 USA. EM gardnerk@mail.nih.gov FU National Cancer Institute; National Institute on Minority Health and Health Disparities FX Supported by the intramural program of the National Cancer Institutes and the National Institute on Minority Health and Health Disparities. NR 119 TC 16 Z9 16 U1 0 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD APR PY 2013 VL 182 IS 4 BP 1055 EP 1064 DI 10.1016/j.ajpath.2013.01.009 PG 10 WC Pathology SC Pathology GA 165NY UT WOS:000320491800003 PM 23438473 ER PT J AU Mossman, BT Shukla, A Heintz, NH Verschraegen, CF Thomas, A Hassan, R AF Mossman, Brooke T. Shukla, Arti Heintz, Nicholas H. Verschraegen, Claire F. Thomas, Anish Hassan, Raffit TI New Insights into Understanding the Mechanisms, Pathogenesis, and Management of Malignant Mesotheliomas SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Review ID GROWTH-FACTOR-RECEPTOR; HISTONE DEACETYLASE INHIBITOR; SIGNAL-REGULATED KINASE-1; TUMOR-SUPPRESSOR GENE; PLEURAL-MESOTHELIOMA; PHASE-II; EXTRAPLEURAL PNEUMONECTOMY; CROCIDOLITE ASBESTOS; PERITONEAL MESOTHELIOMA; LUNG-CANCER AB Malignant mesothetioma (MM) is a relatively rare but devastating tumor that is increasing worldwide. Yet, because of difficulties in early diagnosis and resistance to conventional therapies, MM remains a challenge for pathologists and clinicians to treat. In recent years, much has been revealed regarding the mechanisms of interactions of pathogenic fibers with mesothelial cells, crucial signaling pathways, and genetic and epigenetic events that may occur during the pathogenesis of these unusual, pleiomorphic tumors. These observations support a scenario whereby mesothelial cells undergo a series of chronic injury, inflammation, and proliferation in the Long latency period of MM development that may be perpetuated by durable fibers, the tumor microenvironment, and inflammatory stimuli. One culprit in sustained inflammation is the activated inflammasome, a component of macrophages or mesothelial cells that Leads to production of chemotactic, growth-promoting, and angiogenic cytokines. This information has been vital to designing novel therapeutic approaches for patients with MM that focus on immunotherapy, targeting growth factor receptors and pathways, overcoming resistance to apoptosis, and modifying epigenetic changes. C1 [Mossman, Brooke T.; Shukla, Arti; Heintz, Nicholas H.] Univ Vermont, Coll Med, Dept Pathol, Burlington, VT 05405 USA. [Verschraegen, Claire F.] Univ Vermont, Coll Med, Dept Hematol Oncol, Burlington, VT 05405 USA. [Thomas, Anish] NCI, Med Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Hassan, Raffit] NCI, Mol Biol Lab, Ctr Canc Res, Bethesda, MD 20892 USA. RP Mossman, BT (reprint author), Univ Vermont, Coll Med, Dept Pathol, 89 Beaumont Ave, Burlington, VT 05405 USA. EM brooke.mossman@uvm.edu OI Thomas, Anish/0000-0003-3293-3115 FU Mesothelioma Applied Research Foundation; National Institute of Environmental Health Scienc [T32 ES007122, R01 ES021110]; National Cancer Institute [P01 CA11407]; NTH National Cancer Institute, Center for Cancer Research FX Supported by grants from the Mesothelioma Applied Research Foundation (B.T.M., N.H.H., A.S.), National Institute of Environmental Health Sciences grants T32 ES007122 (B.T.M.) and R01 ES021110 (A.S.), National Cancer Institute grant P01 CA11407 (project 2, B.T.M.), and in part by the Intramural Research Program of the NTH National Cancer Institute, Center for Cancer Research (AT. and R.H.) NR 125 TC 31 Z9 32 U1 0 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD APR PY 2013 VL 182 IS 4 BP 1065 EP 1077 DI 10.1016/j.ajpath.2012.12.028 PG 13 WC Pathology SC Pathology GA 165NY UT WOS:000320491800004 PM 23395095 ER PT J AU Zhou, ST Nissao, E Jackson, IL Leong, W Dancy, L Cuttitta, F Vujaskovic, Z Sunday, ME AF Zhou, Shutang Nissao, Esther Jackson, Isabel L. Leong, Wei Dancy, Lindsay Cuttitta, Frank Vujaskovic, Zeljko Sunday, Mary E. TI Radiation-Induced Lung Injury Is Mitigated by Blockade of Gastrin-Releasing Peptide SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID BRONCHOPULMONARY DYSPLASIA; IN-VITRO; PULMONARY IRRADIATION; NEUROENDOCRINE CELLS; BOMBESIN STIMULATION; THORACIC IRRADIATION; OXIDATIVE STRESS; NEUROMEDIN-C; MOUSE LUNG; FIBROSIS AB Gastrin-releasing peptide (GRP), secreted by pulmonary neuroendocrine cells, mediates oxidant-induced Lung injury in animal models. Considering that GRP blockade abrogates pulmonary inflammation and fibrosis in hyperoxic baboons, we hypothesized that ionizing radiation triggers GRP secretion, contributing to inflammatory and fibrotic phases of radiation-induced lung injury (RiLI). Using C57BL/6 mouse model of pulmonary fibrosis developing >= 20 weeks after high-dose thoracic radiation (15 Gy), we injected small molecule 77427 i.p. approximately 1 hour after radiation then twice weekly for up to 20 weeks. Sham controls were anesthetized and placed in the irradiator without radiation. Lung paraffin sections were immunostained and quantitative image analyses performed. Mice exposed to radiation plus PBS had increased interstitial CD68(+) macrophages 4 weeks after radiation and pulmonary neuroendocrine cells hyperplasia 6 weeks after radiation. Ten weeks Later radiation plus PBS controls had significantly increased pSmad2/3(+) nuclei/cm(2). GRP blockade with 77427 treatment diminished CD68(+), GRP(+), and pSmad2/3(+) cells. Finally, interstitial fibrosis was evident 20 weeks after radiation by immunostaining for alpha-smooth muscle actin and collagen deposition. Treatment with 77427 abrogated interstitial alpha-smooth muscle actin and collagen. Sham mice given 77427 did not differ significantly from PBS controls. Our data are the first to show that GRP blockade decreases inflammatory and fibrotic responses to radiation in mice. GRP blockade is a novel radiation fibrosis mitigating agent that could be clinically useful in humans exposed to radiation therapeutically or unintentionally. C1 [Zhou, Shutang; Nissao, Esther; Leong, Wei; Dancy, Lindsay; Sunday, Mary E.] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA. [Jackson, Isabel L.; Vujaskovic, Zeljko] Duke Univ, Med Ctr, Dept Radiat Oncol, Durham, NC 27710 USA. [Cuttitta, Frank] NCI, Radiat Oncol Brach, Angiogenesis Core Facil, NIH, Gaithersburg, MD USA. RP Zhou, ST (reprint author), Duke Univ, Med Ctr, DUMC Box 3712, Durham, NC 27710 USA. EM shutang.zhou@duke.edu; mary.sunday@duke.edu RI Cuttitta, Frank/B-4758-2016 FU RadCCore Pilot grant [AI-067798-02] FX Supported by a RadCCore Pilot grant AI-067798-02 (M.E.S.) (program director: M. Dewhirst, Center for Medical Countermeasures Against Radiation Injury, University of Pittsburgh, Pittsburgh, PA, and Duke University, Durham, NC). NR 57 TC 4 Z9 5 U1 0 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9440 EI 1525-2191 J9 AM J PATHOL JI Am. J. Pathol. PD APR PY 2013 VL 182 IS 4 BP 1248 EP 1254 DI 10.1016/j.ajpath.2012.12.024 PG 7 WC Pathology SC Pathology GA 165NY UT WOS:000320491800024 PM 23395092 ER PT J AU Yan, CG Tang, HF Gao, HW Johnson, PF Ye, Y Wu, M AF Yan, Chunguang Tang, Huifang Gao, Hongwei Johnson, Peter F. Ye, Yan Wu, Min TI CCAAT/Enhancer-Binding Protein delta Multifaceted Regulator in Respiratory Disease Reply SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Letter ID ACUTE LUNG INJURY; HOST-DEFENSE; PNEUMONIA C1 [Yan, Chunguang; Tang, Huifang; Gao, Hongwei] Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA. [Johnson, Peter F.] NCI, Frederick, MD 21701 USA. [Ye, Yan; Wu, Min] Univ N Dakota, Grand Forks, ND 58201 USA. RP Yan, CG (reprint author), Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA. NR 6 TC 0 Z9 0 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD APR PY 2013 VL 182 IS 4 BP 1459 EP 1460 PG 2 WC Pathology SC Pathology GA 165NY UT WOS:000320491800044 PM 23614144 ER PT J AU Guertin, MJ Lis, JT AF Guertin, Michael J. Lis, John T. TI Mechanisms by which transcription factors gain access to target sequence elements in chromatin SO CURRENT OPINION IN GENETICS & DEVELOPMENT LA English DT Article ID GLUCOCORTICOID-RECEPTOR BINDING; PROTEIN-DNA INTERACTIONS; HISTONE ACETYLATION; CPG ISLANDS; PREFERENTIAL ACCESSIBILITY; NUCLEOSOME ORGANIZATION; REGULATORY ELEMENTS; GENE-REGULATION; HUMAN GENOME; HUMAN-CELLS AB Transcription factors (TF) bind DNA sequence motifs, but the presence of a consensus DNA element is not sufficient to direct TF binding to chromatin. Recent genomic data have revealed that accessibility, as measured by DNase sensitivity and the presence of active histone marks, is necessary for TF binding. DNA sequence provides the initial specification of the accessibility of DNA elements within chromatin that permits TF binding. In yeast, it is known that poly(dA-dT) tracts directly encode low-nucleosome occupancy at promoters. Recent evidence suggests that CpG islands in mammals are inherently refractory to higher-order chromatin structure and remain accessible, despite favoring nucleosome formation in vitro. Taken together, these studies support a model for how accessibility originates and then propagates throughout regulatory cascades and development. C1 [Guertin, Michael J.; Lis, John T.] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA. RP Guertin, MJ (reprint author), NCI, Lab Receptor Biol & Gene Express, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM GuertinMJ@gmail.com; johnlis@cornell.edu FU NIGMS NIH HHS [R01 GM025232] NR 69 TC 26 Z9 26 U1 1 U2 16 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0959-437X J9 CURR OPIN GENET DEV JI Curr. Opin. Genet. Dev. PD APR PY 2013 VL 23 IS 2 BP 116 EP 123 DI 10.1016/j.gde.2012.11.008 PG 8 WC Cell Biology; Genetics & Heredity SC Cell Biology; Genetics & Heredity GA 158GM UT WOS:000319957500007 PM 23266217 ER PT J AU Yohannan, J Munoz, B Mkocha, H Gaydos, CA Bailey, R Lietman, TA Quinn, T West, SK AF Yohannan, Jithin Munoz, Beatriz Mkocha, Harran Gaydos, Charlotte A. Bailey, Robin Lietman, Thomas A. Quinn, Thomas West, Sheila K. TI Can We Stop Mass Drug Administration Prior to 3 Annual Rounds in Communities With Low Prevalence of Trachoma? SO JAMA OPHTHALMOLOGY LA English DT Article ID CHLAMYDIA-TRACHOMATIS; ANTIBIOTIC-TREATMENT; RANDOMIZED-TRIAL; OCULAR CHLAMYDIA; AZITHROMYCIN; INFECTION; TANZANIA; HOUSEHOLDS; ETHIOPIA; VILLAGE AB Importance: The World Health Organization recommends at least 3 annual mass drug administrations (MDAs) of azithromycin in places where the prevalence of follicular trachoma (FT) is greater than 10%. However, stopping MDA prior to 3 rounds, if monitoring indicates an absence of infection with Chlamydia trachomatis even if FT persists, may be more cost-effective. Objective: To determine the prevalence of infection in communities randomized to 3 rounds of annual MDAs with azithromycin compared with communities randomized to a stopping rule, where MDA could cease if the infection rate was low. Design: A 1:1 community randomized trial comparing usual care with a cessation rule. The Partnership for the Rapid Elimination of Trachoma-Ziada Trial was conducted from February 1, 2010, through September 1, 2011. Setting: Sixteen communities in Tanzania with trachoma prevalence rates between 10% and 20%. Participants: A total of 100 children aged 5 years or younger randomly drawn from each community. Children had to reside in an eligible community, have no ocular condition that prevented trachoma grading or ocular specimen collection, and have a guardian who could provide consent for participation. Interventions: Cessation of MDA with azithromycin if the community had no infection in their sample at 6 months or 18 months. Main Outcome Measure: The prevalence of C trachomatis at 18 months. Results: None of the intervention communities met criteria to stop MDA based on the 6-month or 18-month survey; all, as well as the usual care communities, were scheduled for a third MDA round. There was no difference in infection (2.9% vs 4.7%; P=.25) between the usual care and cessation rule communities at 18 months. Conclusions and Relevance: In this setting, communities with low (10%-20%) initial prevalence of active trachoma did not have MDA stopped before 3 annual rounds on the basis of monitoring for infection. Infection with C trachomatis in communities with average trachoma rates at 12% to 13% cannot be eliminated before 3 rounds of MDA with azithromycin. C1 [Yohannan, Jithin; Munoz, Beatriz; West, Sheila K.] Johns Hopkins Univ, Dana Ctr Prevent Ophthalmol, Baltimore, MD USA. [Gaydos, Charlotte A.] Johns Hopkins Univ, Int Chlamydia Lab, Dept Infect Dis, Baltimore, MD USA. [Quinn, Thomas] NIAID, Bethesda, MD 20892 USA. [Mkocha, Harran] Kongwa Trachoma Project, Kongwa, Tanzania. [Bailey, Robin] London Sch Hyg & Trop Med, London WC1, England. [Lietman, Thomas A.] Univ Calif San Francisco, Proctor Fdn, San Francisco, CA 94143 USA. RP West, SK (reprint author), Wilmer Eye Inst, Rm 129,600 N Wolfe St, Baltimore, MD 21287 USA. EM shwest@jhmi.edu FU Bill and Melinda Gates Foundation FX This study was funded by a grant from the Bill and Melinda Gates Foundation. NR 20 TC 5 Z9 5 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-6165 J9 JAMA OPHTHALMOL JI JAMA Ophthalmol. PD APR PY 2013 VL 131 IS 4 BP 431 EP 436 DI 10.1001/jamaophthalmol.2013.2356 PG 6 WC Ophthalmology SC Ophthalmology GA 163JH UT WOS:000320331600002 PM 23392481 ER PT J AU Bogdanos, DP Gao, B Gershwin, ME AF Bogdanos, Dimitrios P. Gao, Bin Gershwin, M. Eric TI Liver Immunology SO COMPREHENSIVE PHYSIOLOGY LA English DT Article ID PRIMARY BILIARY-CIRRHOSIS; HEPATITIS-C-VIRUS; SINUSOIDAL-ENDOTHELIAL-CELLS; REGULATORY T-CELLS; PRIMARY SCLEROSING CHOLANGITIS; PYRUVATE-DEHYDROGENASE COMPLEX; CHRONIC ACTIVE HEPATITIS; GENOME-WIDE ASSOCIATION; INTERCELLULAR-ADHESION MOLECULE-1; LISTERIA-MONOCYTOGENES INFECTION AB The liver is the largest organ in the body and is generally regarded by nonimmunologists as having little or no lymphoid function. However, such is far from accurate. This review highlights the importance of the liver as a lymphoid organ. Firstly, we discuss experimental data surrounding the role of liver as a lymphoid organ. The liver facilitates tolerance rather than immunoreactivity, which protects the host from antigenic overload of dietary components and drugs derived from the gut and it is instrumental to fetal immune tolerance. Loss of liver tolerance leads to autoaggressive phenomena, which if not controlled by regulatory lymphoid populations, may lead to the induction of autoimmune liver diseases. Liver-related lymphoid subpopulations also act as critical antigen-presenting cells. The study of the immunological properties of liver and delineation of the microenvironment of the intrahepatic milieu in normal and diseased livers provides a platform to understand the hierarchy of a series of detrimental events that lead to immune-mediated destruction of the liver and the rejection of liver allografts. The majority of emphasis within this review will be on the normal mononuclear cell composition of the liver. However, within this context, we will discuss selected, but not all, immune-mediated liver disease and attempt to place these data in the context of human autoimmunity. (C) 2013 American Physiological Society. C1 [Bogdanos, Dimitrios P.] Kings Coll Hosp London, Kings Coll London Sch Med, Inst Liver Studies Transplantat Immunol & Mucosal, London, England. [Gao, Bin] NIAAA, Lab Liver Dis, NIH, Bethesda, MD USA. [Gershwin, M. Eric] Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA. RP Gershwin, ME (reprint author), Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA. EM megershwin@ucdavis.edu FU Higher Education Funding Council for England; National Institutes of Health [DK39588] FX D.P. Bogdanos is a recipient of a CLS award from the Higher Education Funding Council for England; the authors regret that several important original and review papers could not be cited due to reference number and space constraints. Financial support provided by National Institutes of Health grant DK39588. NR 439 TC 16 Z9 17 U1 0 U2 16 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 2040-4603 J9 COMPR PHYSIOL JI Compr. Physiol. PD APR PY 2013 VL 3 IS 2 BP 567 EP 598 DI 10.1002/cphy.c120011 PG 32 WC Physiology SC Physiology GA 157NY UT WOS:000319906100001 PM 23720323 ER PT J AU Abeliovich, H Zarei, M Rigbolt, KTG Youle, RJ Dengjel, J AF Abeliovich, Hagai Zarei, Mostafa Rigbolt, Kristoffer T. G. Youle, Richard J. Dengjel, Joern TI Mitophagy as a quality control mechanism in Saccharomyces cerevisiae SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Abeliovich, Hagai] Hebrew Univ Jerusalem, IL-76100 Rehovot, Israel. [Zarei, Mostafa; Rigbolt, Kristoffer T. G.; Dengjel, Joern] Freiburg Inst Adv Studies, Freiburg, Germany. [Youle, Richard J.] NINDS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 994.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500343 ER PT J AU Abeykoon, AH Noinaj, N Chao, CC Wang, GH Chock, PB Gucek, M Ching, WM Buchanan, SK Yang, DCH AF Abeykoon, Amila H. Noinaj, Nicholas Chao, Chien-Chung Wang, Guanghui Chock, P. Boon Gucek, Marjan Ching, Wei-Mei Buchanan, Susan K. Yang, David C. H. TI Structural Insights of Outer Membrane Protein Methyltransferases from Rickettsia SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Abeykoon, Amila H.; Yang, David C. H.] Georgetown Univ, Washington, DC USA. [Noinaj, Nicholas; Buchanan, Susan K.] NIDDK, NIH, Bethesda, MD USA. [Wang, Guanghui; Chock, P. Boon; Gucek, Marjan] NHLBI, NIH, Bethesda, MD 20892 USA. [Chao, Chien-Chung; Ching, Wei-Mei] USN, Viral & Rickettsial Dis Dept, Med Res Ctr, Silver Spring, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 561.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504021 ER PT J AU Ambs, S AF Ambs, Stefan TI Novel targets in advanced breast cancer in African American patients SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Ambs, Stefan] NCI, LHC, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 214.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502439 ER PT J AU Andrews, KW Roseland, JM Middleton, A Solomon, A Palachuvattil, J Dang, PT Holden, JM Pehrsson, PR Dwyer, JT Bailey, RL Betz, JM Costello, RB Saldanha, LG Hardy, CJ Gahche, JJ Emenaker, NJ Douglass, L AF Andrews, K. W. Roseland, J. M. Middleton, A. Solomon, A. Palachuvattil, J. Dang, P. T. Holden, J. M. Pehrsson, P. R. Dwyer, J. T. Bailey, R. L. Betz, J. M. Costello, R. B. Saldanha, L. G. Hardy, C. J. Gahche, J. J. Emenaker, N. J. Douglass, L. TI Chemical analysis of omega-3 (n-3) fatty acid supplements for the Dietary Supplement Ingredient Database (DSID) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Andrews, K. W.; Roseland, J. M.; Middleton, A.; Solomon, A.; Palachuvattil, J.; Dang, P. T.; Holden, J. M.; Pehrsson, P. R.] USDA, Nutrient Data Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Dwyer, J. T.; Bailey, R. L.; Betz, J. M.; Costello, R. B.; Saldanha, L. G.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Hardy, C. J.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD USA. [Gahche, J. J.] US Dept Hlth & Human Serv, Natl Ctr Hlth Stat, Ctr Dis Control, Hyattsville, MD USA. [Emenaker, N. J.] NCI, Nutr Sci Res Grp, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504129 ER PT J AU Ashour, FS Raiten, D Ross, C Meydani, S AF Ashour, Fayrouz Sakr Raiten, Daniel Ross, Catharine Meydani, Simin TI Inflammation and Nutritional Science for Programs/Policies: Interpretation of Research Evidence SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Ashour, Fayrouz Sakr; Raiten, Daniel] NICHD, NIH, Bethesda, MD USA. [Ross, Catharine] Penn State Univ, State Coll, PA USA. [Meydani, Simin] Tufts Univ, Human Nutr Res Ctr Aging, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Meydani, Simin] Tufts Univ, Sackler Grad Sch, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 846.16 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860501595 ER PT J AU Assumpcao, T Ma, DY Schwarz, A Reiter, K Santana, JM Andersen, JF Ribeiro, JMC Nardone, G Yu, LL Francischetti, IMB AF Assumpcao, Teresa Ma, Dongying Schwarz, Alexandra Reiter, Karine Santana, Jaime M. Andersen, John F. Ribeiro, Jose M. C. Nardone, Glenn Yu, Lee L. Francischetti, Ivo M. B. TI Salivary antigen-5/CAP family members are Cu2+-dependent antioxidant enzymes which negatively modulate platelet function SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Assumpcao, Teresa; Ma, Dongying; Andersen, John F.; Ribeiro, Jose M. C.; Francischetti, Ivo M. B.] NIAID, Lab Malaria & Vector Res, NIH, Bethesda, MD 20892 USA. [Schwarz, Alexandra] Acad Sci Czech Republic, Inst Parasitol, Ctr Biol, Lab Genom & Prote Dis Vectors, CR-37005 Ceske Budejovice, Czech Republic. [Reiter, Karine] NIAID, Malaria Vaccine Dev Branch, NIH, Rockville, MD USA. [Nardone, Glenn] NIAID, Res Technol Branch, NIH, Rockville, MD USA. [Santana, Jaime M.] Univ Brasilia, Pathogen Host Interface Lab, Dept Cell Biol, Brasilia, DF, Brazil. [Yu, Lee L.] NIST, Div Chem Sci, Gaithersburg, MD 20899 USA. RI ma, dongying/D-8623-2012; Ribeiro, Jose/J-7011-2015; Yu, Lee/N-7263-2015 OI Yu, Lee/0000-0002-8043-6853 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb173 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505593 ER PT J AU Bailey, RL Durazo-Arvizu, R Carmel, R Green, R Pfeiffer, CM Sempos, CT Yetley, EA AF Bailey, Regan L. Durazo-Arvizu, Ramon Carmel, Ralph Green, Ralph Pfeiffer, Christine M. Sempos, Christopher T. Yetley, Elizabeth A. TI Modeling an MMA-derived inflection point for serum vitamin B12 (SB12) in the US SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Bailey, Regan L.; Sempos, Christopher T.; Yetley, Elizabeth A.] NIH, ODS, Bethesda, MD 20892 USA. [Durazo-Arvizu, Ramon] Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USA. [Carmel, Ralph] NY Methodist Hosp, Brooklyn, NY USA. [Carmel, Ralph] Weill Cornell Med Coll, New York, NY USA. [Green, Ralph] UC Davis Med Ctr, Sacramento, CA USA. [Pfeiffer, Christine M.] CDC, NCEH, Atlanta, GA 30333 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 848.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505477 ER PT J AU Bailey, RL Gahche, JJ Thomas, PR Dwyer, JT AF Bailey, Regan L. Gahche, Jaime J. Thomas, Paul R. Dwyer, Johanna T. TI Why children use dietary supplements SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Bailey, Regan L.; Thomas, Paul R.; Dwyer, Johanna T.] NIH, ODS, Bethesda, MD 20892 USA. [Gahche, Jaime J.] CDC, NCHS, Hyattsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500098 ER PT J AU Balasubramanian, R Maruoka, H Jayasekara, SMP Gao, ZG Jacobson, KA AF Balasubramanian, Ramachandran Maruoka, Hiroshi Jayasekara, Suresh M. P. Gao, Zhanguo Jacobson, Kenneth A. TI AMP-ACTIVATED PROTEIN KINASE AS REGULATOR OF P2Y6 RECEPTOR-INDUCED INSULIN SECRETION IN MOUSE PANCREATIC BETA CELLS SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Balasubramanian, Ramachandran; Maruoka, Hiroshi; Jayasekara, Suresh M. P.; Gao, Zhanguo; Jacobson, Kenneth A.] NIDDK, LBC, MRS, NIH, Bethesda, MD USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1169.15 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503264 ER PT J AU Banerjee, A Abdelmegeed, MA Jang, S Song, BJ AF Banerjee, Atrayee Abdelmegeed, Mohamed A. Jang, Sehwan Song, Byoung Joon TI Oxidative stress and ER stress induce Zidovudine (AZT)-mediated hepatic lipid accumulation SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Banerjee, Atrayee; Abdelmegeed, Mohamed A.; Jang, Sehwan; Song, Byoung Joon] NIAAA, Lab Membrane Biochem & Biophys, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1144.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500427 ER PT J AU Best, KA Bone, D Gros, R Hammond, J AF Best, Keisha Arielle Bone, Derek Gros, Robert Hammond, James TI Characterization of the Vascular Phenotype of the Equilibrative Nucleoside Transporter 1 Knockout Mouse SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Best, Keisha Arielle; Gros, Robert] Western Univ, London, ON, Canada. [Bone, Derek] NIH, Bethesda, MD 20892 USA. [Hammond, James] Univ Alberta, Edmonton, AB, Canada. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 676.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500662 ER PT J AU Best, KA Bone, D Feng, QP Gros, R Hammond, J AF Best, Keisha Arielle Bone, Derek Feng, Qingping Gros, Robert Hammond, James TI Characterization of the Vascular Phenotype of the Equilibrative Nucleoside Transporter 1 Knockout Mouse SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Best, Keisha Arielle; Feng, Qingping; Gros, Robert] Univ Western Ontario, London, ON, Canada. [Bone, Derek] Natl Inst Diabet, Mol Signaling Sect, Bioorgan Chem Lab, Bethesda, MD USA. [Bone, Derek] NIH, Bethesda, MD 20892 USA. [Hammond, James] Univ Alberta, Edmonton, AB, Canada. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb594 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502067 ER PT J AU Beydoun, H Khanal, S Zonderman, AB Beydoun, M AF Beydoun, Hind Khanal, Suraj Zonderman, Alan B. Beydoun, May TI Is Exposure to Bisphenol-A and Phthalates Associated with Obesity, Metabolic Disturbances and Insulin Resistance among US adults? SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Beydoun, Hind; Khanal, Suraj] Eastern Virginia Med Sch, Norfolk, VA 23501 USA. [Zonderman, Alan B.; Beydoun, May] NIA, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 630.24 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505156 ER PT J AU Beydoun, MA Gamaldo, A Beydoun, HA Tanaka, T Tucker, KL Talegawkar, S Ferrucci, L Zonderman, AB AF Beydoun, May A. Gamaldo, Alyssa Beydoun, Hind A. Tanaka, Toshiko Tucker, Katherine L. Talegawkar, Sameera Ferrucci, Luigi Zonderman, Alan B. TI Caffeine, alcohol and overall nutrient adequacy are associated with longitudinal cognitive performance among US adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Beydoun, May A.; Gamaldo, Alyssa; Zonderman, Alan B.] NIA, Lab Populat Sci, NIH, IRP, Baltimore, MD 21224 USA. [Beydoun, Hind A.] Eastern Virginia Med Sch, Gradulate Program Publ Hlth, Norfolk, VA 23501 USA. [Tanaka, Toshiko; Ferrucci, Luigi] NIA, Translat Gerontol Branch, NIH, IRP, Baltimore, MD 21224 USA. [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Talegawkar, Sameera] Johns Hopkins Sch Publ Hlth, Dept Int Hlth, Ctr Human Nutr, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 346.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505444 ER PT J AU Beydoun, MA Beydoun, HA Kitner-Triolo, MH Kaufman, JS Evans, MK Zonderman, AB AF Beydoun, May A. Beydoun, Hind A. Kitner-Triolo, Melissa H. Kaufman, Jay S. Evans, Michele K. Zonderman, Alan B. TI Thyroid hormones and their associations with cognitive function: moderation by sex, race and depressive symptoms SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Beydoun, May A.; Evans, Michele K.; Zonderman, Alan B.] NIA, Lab Populat Sci, NIH, IRP, Baltimore, MD 21224 USA. [Kitner-Triolo, Melissa H.] NIA, Lab Behav Neurosci, NIH, IRP, Baltimore, MD 21224 USA. [Beydoun, Hind A.] Eastern Virginia Med Sch, Grad Program Publ Hlth, Norfolk, VA 23501 USA. [Kaufman, Jay S.] McGill Univ, Dept Epidemiol Biostat & Occupat Hlth, Montreal, PQ, Canada. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 840.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503558 ER PT J AU Blumberg, PM AF Blumberg, Peter M. TI Natural products as powerful tools for drug discovery: insights from the phorbol esters SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Blumberg, Peter M.] NCI, Lab Canc Biol & Genet, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 83.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500259 ER PT J AU Bolger, SJ Hurtado, PAG Hoffert, JD Saeed, F Pisitkun, T Knepper, MA AF Bolger, Steven J. Hurtado, Patricia A. Gonzales Hoffert, Jason D. Saeed, Fahad Pisitkun, Trairak Knepper, Mark A. TI Quantitative phosphoproteomics implicates clusters of proteins involved in cell-cell adhesion and transcriptional regulation in the vasopressin signaling network SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Bolger, Steven J.; Hurtado, Patricia A. Gonzales; Hoffert, Jason D.; Saeed, Fahad; Pisitkun, Trairak; Knepper, Mark A.] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 597.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500226 ER PT J AU Bowman, G Dodge, H Kaye, J Quinn, J AF Bowman, Gene Dodge, HIroko Kaye, Jeffrey Quinn, Joseph TI Omega 3 Fatty Acids and White Matter Mediated Cognitive Decline in Older Adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Bowman, Gene; Dodge, HIroko; Kaye, Jeffrey; Quinn, Joseph] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Bowman, Gene] NIA, OHSU, Layton Aging & Alzheimers Dis Ctr, Portland, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 346.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504234 ER PT J AU Briscione, M Serafine, K Merluzzi, A Rice, K Riley, A AF Briscione, Maria Serafine, Katherine Merluzzi, Andrew Rice, Kenner Riley, Anthony TI The effects of tropisetron on cocaine-induced conditioned taste aversions SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Briscione, Maria; Merluzzi, Andrew; Riley, Anthony] American Univ, Washington, DC 20016 USA. [Serafine, Katherine] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA. [Rice, Kenner] NIDA, Chem Biol Res Branch, Baltimore, MD USA. [Rice, Kenner] NIAAA, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1098.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502365 ER PT J AU Byun, JS Gardner, K AF Byun, Jung S. Gardner, Kevin TI CTBP regulates the early SEC assembly prior to onset of transcription elongation SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Byun, Jung S.; Gardner, Kevin] NCI, Genet Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 837.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502013 ER PT J AU Cho, HY Miller-DeGraff, L Perrow, L Yamamoto, M Kleeberger, SR AF Cho, Hye-Youn Miller-DeGraff, Laura Perrow, Ligon Yamamoto, Masayuki Kleeberger, Steven R. TI Effect of prenatal antioxidant sulforaphane on fetal transcriptomics in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Cho, Hye-Youn; Miller-DeGraff, Laura; Perrow, Ligon; Kleeberger, Steven R.] NIEHS, Lab Resp Biol, NIH, Res Triangle Pk, NC 27709 USA. [Yamamoto, Masayuki] Tohoku Univ, Grad Sch Med, Sendai, Miyagi 980, Japan. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1142.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504028 ER PT J AU Chou, CL Han, L Pisitkun, T Knepper, MA AF Chou, C. L. Han, L. Pisitkun, T. Knepper, M. A. TI Urea channel Slc14a2 interactome in rat inner medullary collecting duct SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Chou, C. L.; Han, L.; Pisitkun, T.; Knepper, M. A.] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1210.19 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505440 ER PT J AU Chun, LS Free, RB Doyle, TB Huang, XP Sibley, DR AF Chun, Lani Sunil Free, R. Benjamin Doyle, Trevor B. Huang, Xi-Ping Sibley, David R. TI Investigation of the D-1-D-2 dopamine receptor heteromer reveals a complex signaling mechanism not limited to G(q) protein activation SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Chun, Lani Sunil; Free, R. Benjamin; Doyle, Trevor B.; Sibley, David R.] NINDS, Mol Neuropharmacol Sect, NIH, Bethesda, MD 20892 USA. [Chun, Lani Sunil] Johns Hopkins Univ, CMDB Program, Baltimore, MD USA. [Huang, Xi-Ping] Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 881.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500610 ER PT J AU Ciappio, ED Rochman, M Horsch, M Beckers, J Krausz, KW Bonzo, JA Gonzalez, FJ Bustin, M AF Ciappio, Eric D. Rochman, Mark Horsch, Marion Beckers, Johannes Krausz, Kristopher W. Bonzo, Jessica A. Gonzalez, Frank J. Bustin, Michael TI Targeted disruption of the nucleosomal binding protein HMGN5 results in altered glutathione metabolism and mild hepatic dysfunction SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Ciappio, Eric D.; Rochman, Mark; Krausz, Kristopher W.; Bonzo, Jessica A.; Gonzalez, Frank J.; Bustin, Michael] NCI, Lab Metab, Bethesda, MD 20892 USA. [Horsch, Marion; Beckers, Johannes] German Res Ctr Environm Hlth, German Mouse Clin, Neuherberg, Germany. RI Bustin, Michael/G-6155-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1073.12 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502601 ER PT J AU Claxton, JS Liu, G Sandoval, PC Chou, CL Knepper, MA AF Claxton, J. S. Liu, G. Sandoval, P. C. Chou, C-L Knepper, M. A. TI Protein carbamylation is a physiological post-translational modification in the renal inner medulla SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Claxton, J. S.; Liu, G.; Sandoval, P. C.; Chou, C-L; Knepper, M. A.] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1111.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504586 ER PT J AU Conroy, JL Doyle, TB Sibley, DR AF Conroy, Jennie Lynn Doyle, Trevor B. Sibley, David R. TI Identification of substituted benzazepines as functionally selective ligands of the D-1 dopamine receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Conroy, Jennie Lynn; Doyle, Trevor B.; Sibley, David R.] NINDS, Mol Neuropharmacol Sect, NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 655.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860501229 ER PT J AU Costello, RB Dwyer, JT Bailey, RL French, S AF Costello, Rebecca B. Dwyer, Johanna T. Bailey, Regan L. French, Steven TI Use of Highly Fortified Foods Among US Adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Costello, Rebecca B.; Dwyer, Johanna T.; Bailey, Regan L.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [French, Steven] Nat Mkt Inst, Harleysville, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 848.9 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504545 ER PT J AU Davaasambuu, G Troisi, R AF Davaasambuu, Ganmaa Troisi, Rebecca TI Prevalence of Vitamin D Deficiency in Healthy, Reproductive Age Women Living in Mongolia SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Davaasambuu, Ganmaa] Harvard, Nutr, Boston, MA USA. [Troisi, Rebecca] NCI, NIH, Lebanon, NH USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 859.15 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504072 ER PT J AU DesJardin, JT Notari, L Becerra, SP Subramanian, P AF DesJardin, Jacqueline Talea Notari, Luigi Becerra, S. Patricia Subramanian, Preeti TI Analyses of Patatin-like Phospholipase Domain-containing 2 (PNPLA2) Transcripts: Discrepancies between Expressed Sequence Tag (EST) and Experimental Data SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [DesJardin, Jacqueline Talea; Notari, Luigi; Becerra, S. Patricia; Subramanian, Preeti] NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb191 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860506172 ER PT J AU Dhurandhar, NV Dhurandhar, EJ Ingram, DK de Cabo, R Mattison, JA AF Dhurandhar, Nikhil V. Dhurandhar, Emily J. Ingram, Donald K. de Cabo, Rafa Mattison, Julie A. TI Natural infection of human adenovirus 36 in rhesus monkeys is associated with gain in body fat, yet, reduction in fasting glucose SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Dhurandhar, Nikhil V.; Ingram, Donald K.] Pennington Biomed Res Ctr, Baton Rouge, LA USA. [Dhurandhar, Emily J.] Univ Alabama Birmingham, Birmingham, AL USA. [de Cabo, Rafa; Mattison, Julie A.] NIA, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 48.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504138 ER PT J AU Dmitrieva, NI Burg, MB AF Dmitrieva, Natalia I. Burg, Maurice B. TI Elevation of extracellular NaCl increases secretion of von Willebrand Factor from endothelial cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Dmitrieva, Natalia I.; Burg, Maurice B.] NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 686.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504393 ER PT J AU Duke, AN Green, HL Newman, AH Nader, MA AF Duke, Angela N. Green, Heather L. Newman, Amy H. Nader, Michael A. TI The role of dopamine D3 receptors in the discriminative stimulus effects of quinpirole, cocaine, and methamphetamine in rhesus monkeys SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Duke, Angela N.; Green, Heather L.; Nader, Michael A.] Wake Forest Univ, Bowman Gray Sch Med, Dept Physiol & Pharmacol, Winston Salem, NC 27103 USA. [Newman, Amy H.] NIDA IRP, Med Chem Sect, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 659.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503083 ER PT J AU Dwyer, J Andrews, KW Bailey, RL Betz, JM Burt, VL Costello, RB Emenaker, NJ Gahche, JJ Hardy, CJ Pehrsson, PR Roseland, JM Saldanha, LG AF Dwyer, Johanna Andrews, Karen W. Bailey, Regan L. Betz, Joseph M. Burt, Vicki L. Costello, Rebecca B. Emenaker, Nancy J. Gahche, Jaime J. Hardy, Constance J. Pehrsson, Pamela R. Roseland, Janet M. Saldanha, Leila G. TI Progress in the Development of Federal Resources to Assess Dietary Supplement (DS) Exposures SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Dwyer, Johanna; Bailey, Regan L.; Betz, Joseph M.; Costello, Rebecca B.; Saldanha, Leila G.] ODS, NIH, Bethesda, MD USA. [Emenaker, Nancy J.] NCI, NIH, Bethesda, MD 20892 USA. [Andrews, Karen W.; Pehrsson, Pamela R.; Roseland, Janet M.] ARS, USDA, Beltsville, MD USA. [Burt, Vicki L.; Gahche, Jaime J.] CDC, NCHS, Hyattsville, MD USA. [Hardy, Constance J.] CFSAN, FDA, College Pk, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503526 ER PT J AU Dwyer, JT Jacques, PF Rogers, GT Sempos, C Bailey, RL AF Dwyer, Johanna T. Jacques, Paul F. Rogers, Gail T. Sempos, Christopher Bailey, Regan L. TI Parent and child use of dietary supplements are associated SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Dwyer, Johanna T.; Bailey, Regan L.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Dwyer, Johanna T.; Jacques, Paul F.; Rogers, Gail T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, Paul F.] Friedman Sch Nutr Sci & Policy, Boston, MA USA. [Sempos, Christopher] NIH, Off Dietary Supplements, Betheda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500518 ER PT J AU Emery, AC Eiden, MV Mustafa, T Eiden, LE AF Emery, Andrew Clayton Eiden, Maribeth V. Mustafa, Tomris Eiden, Lee E. TI A new molecular sensor controlling cAMP activation of ERK SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Emery, Andrew Clayton; Mustafa, Tomris; Eiden, Lee E.] NIMH, SMN, IRP, Bethesda, MD 20892 USA. [Eiden, Maribeth V.] NIMH, SDGT, IRP, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb555 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504319 ER PT J AU Esser, L Zhou, F Yu, CA Xia, D AF Esser, Lothar Zhou, Fei Yu, Chang-An Xia, Di TI The motion control of the iron-sulfur-protein in complex III SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Esser, Lothar; Zhou, Fei; Xia, Di] NCI, NIH, Bethesda, MD 20892 USA. [Yu, Chang-An] Univ Oklahoma Stillwater, Stillwater, OK USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 803.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502513 ER PT J AU Fang, EF Scheibye-Knudsen, M Croteau, DL Bohr, VA AF Fang, Evandro Fei Scheibye-Knudsen, Morten Croteau, Deborah L. Bohr, Vilhelm A. TI Xeroderma pigmentosum group A protein modulates mitophagy through regulation of mitochondrial-associated proteins SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Fang, Evandro Fei; Scheibye-Knudsen, Morten; Croteau, Deborah L.; Bohr, Vilhelm A.] NIA, Lab Mol Gerontol, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb468 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503135 ER PT J AU Ford, DJ Jensen, GL Bailey, R Smiciklas-Wright, H Erickson, P Wood, C Still, C Coffman, D Hartman, TJ AF Ford, Dara J. Jensen, Gordon L. Bailey, Regan Smiciklas-Wright, Helen Erickson, Pennifer Wood, Craig Still, Christopher Coffman, Donna Hartman, Terryl J. TI The association between diet quality, BMI and health-related quality of life in the Geisinger Rural Aging Study (GRAS) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Ford, Dara J.; Jensen, Gordon L.; Smiciklas-Wright, Helen; Hartman, Terryl J.] Penn State Univ, University Pk, PA 16802 USA. [Bailey, Regan] NIH, Off Dietary Supplements, Rockville, MD USA. [Erickson, Pennifer] Penn State Hershey, Publ Hlth Sci, State Coll, PA USA. [Wood, Craig; Still, Christopher] Geisinger Hlth Syst, Geisinger Ctr Hlth Res, Danville, PA USA. [Wood, Craig; Still, Christopher] Geisinger Hlth Syst, Obes Inst, Danville, PA USA. [Coffman, Donna] Penn State Univ, Methodol Ctr, State Coll, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 245.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500493 ER PT J AU Free, RB Xiao, JB Doyle, T Conroy, J Titus, S Bryant-Genevier, M Southall, N Hu, X Ferrer, M Javitch, JA Marugan, JJ Sibley, DR AF Free, R. Benjamin Xiao, Jingbo Doyle, Trevor Conroy, Jennie Titus, Steve Bryant-Genevier, Melanie Southall, Noel Hu, Xin Ferrer, Marc Javitch, Jonathan A. Marugan, Juan J. Sibley, David R. TI Discovery, characterization, and optimization of highly selective D-2 dopaminergic antagonists SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Free, R. Benjamin; Doyle, Trevor; Conroy, Jennie; Sibley, David R.] NINDS, MNS, NIH, Bethesda, MD 20892 USA. [Xiao, Jingbo; Titus, Steve; Bryant-Genevier, Melanie; Southall, Noel; Hu, Xin; Ferrer, Marc; Marugan, Juan J.] NIH, Natl Ctr Adv Translat Sci, Rockville, MD USA. [Javitch, Jonathan A.] Columbia Univ Coll Phys & Surg, Ctr Mol Recognit, New York, NY 10032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 655.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500523 ER PT J AU Gahche, JJ Bailey, R Burt, V Dwyer, J AF Gahche, Jaime Jacqueline Bailey, Regan Burt, Vicki Dwyer, Johanna TI Dietary Supplement Use Among Older Adults in the United States, NHANES 2007-2010 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Gahche, Jaime Jacqueline; Burt, Vicki] Ctr Dis Control & Prevent, Div Natl Hlth & Nutr Examinat Surveys, Natl Ctr Hlth Stat, Hyattsville, MD USA. [Bailey, Regan; Dwyer, Johanna] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504007 ER PT J AU Gee, DL Bailey, RL AF Gee, David L. Bailey, Regan L. TI Prevalence of metabolic syndrome (MetS) and hyperglycemia in US adults: NHANES 2003-06 and 2007-10 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Gee, David L.] Cent Washington Univ, Ellensburg, WA USA. [Bailey, Regan L.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 622.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505348 ER PT J AU Gladwell, W Middleton, L Cook, D Kleeberger, SR Ciencewicki, J AF Gladwell, Wesley, II Middleton, Lance Cook, Donald Kleeberger, Steven R. Ciencewicki, Jon TI Hyperoxia enhances response to respiratory syncytial virus (RSV) infection SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Gladwell, Wesley, II; Cook, Donald; Kleeberger, Steven R.; Ciencewicki, Jon] NIEHS, Lab Resp Biol, NIH, Res Triangle Pk, NC USA. [Middleton, Lance] Stanford Univ, Sch Med, Stanford, CA 94305 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1212.12 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504224 ER PT J AU Gould, RW Nedelcovych, MT Grannan, MD Bubser, M Wood, MR Lindsley, CW Xiang, ZX Wess, J Conn, PJ Jones, CK AF Gould, Robert W. Nedelcovych, Michael T. Grannan, Michael D. Bubser, Michael Wood, Michael R. Lindsley, Craig W. Xiang, Zixiu Wess, Jurgen Conn, P. Jeffrey Jones, Carrie K. TI Effects of M1 and M4 muscarinic acetylcholine receptor positive allosteric modulators on sleep and cognition in rodents SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Gould, Robert W.; Nedelcovych, Michael T.; Grannan, Michael D.; Bubser, Michael; Wood, Michael R.; Lindsley, Craig W.; Xiang, Zixiu; Conn, P. Jeffrey; Jones, Carrie K.] Vanderbilt Univ, Vanderbilt Ctr Neurosci Drug Discovery, Nashville, TN USA. [Gould, Robert W.; Nedelcovych, Michael T.; Grannan, Michael D.; Bubser, Michael; Wood, Michael R.; Lindsley, Craig W.; Xiang, Zixiu; Conn, P. Jeffrey; Jones, Carrie K.] Vanderbilt Univ, Nashville, TN USA. [Wess, Jurgen] NIDDKD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 13 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 661.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503084 ER PT J AU Guenther, PM Kirkpatrick, SI Krebs-Smith, SM Reedy, J Buckman, DW Dodd, KW Carroll, RJ AF Guenther, Patricia M. Kirkpatrick, Sharon I. Krebs-Smith, Susan M. Reedy, Jill Buckman, Dennis W. Dodd, Kevin W. Carroll, Raymond J. TI Evaluation of the Healthy Eating Index-2010 ( HEI-2010) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Guenther, Patricia M.] USDA Ctr Nutr Policy & Promot, Alexandria, VA USA. [Kirkpatrick, Sharon I.; Krebs-Smith, Susan M.; Reedy, Jill; Dodd, Kevin W.] NCI, Bethesda, MD 20892 USA. [Buckman, Dennis W.] Information Management Serv Inc, Rockville, MD USA. [Carroll, Raymond J.] Texas A&M Univ, College Stn, TX USA. NR 0 TC 0 Z9 0 U1 1 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 230.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503290 ER PT J AU Guertin, KA Agler, AH LaBarre, J Parker, RS Kristal, AR Arnold, KB Hartline, J Goodman, PJ Tangen, CM Minasian, LM Lippman, SM Klein, E Cassano, PA AF Guertin, K. A. Agler, A. H. LaBarre, J. Parker, R. S. Kristal, A. R. Arnold, K. B. Hartline, J. Goodman, P. J. Tangen, C. M. Minasian, L. M. Lippman, S. M. Klein, E. Cassano, P. A. TI THE RESPONSE TO VITAMIN E SUPPLEMENTATION SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Guertin, K. A.; Agler, A. H.; LaBarre, J.; Parker, R. S.; Cassano, P. A.] Cornell Univ, Ithaca, NY USA. [Kristal, A. R.; Tangen, C. M.] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Arnold, K. B.; Hartline, J.; Goodman, P. J.] SWOG Stat Ctr, Seattle, WA USA. [Minasian, L. M.] NCI, Bethesda, MD 20892 USA. [Lippman, S. M.] Univ Calif San Diego, La Jolla, CA 92093 USA. [Klein, E.] Cleveland Clin, Cleveland, OH 44106 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 242.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503197 ER PT J AU Guertin, KA Moore, SC Sampson, JN Stolzenberg-Solomon, RZ Sinha, R Cross, AJ AF Guertin, K. A. Moore, S. C. Sampson, J. N. Stolzenberg-Solomon, R. Z. Sinha, R. Cross, A. J. TI Self-Reported Dietary Intake and the Human Metabolome SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Guertin, K. A.; Moore, S. C.; Sampson, J. N.; Stolzenberg-Solomon, R. Z.; Sinha, R.; Cross, A. J.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. RI Sinha, Rashmi/G-7446-2015 OI Sinha, Rashmi/0000-0002-2466-7462 NR 0 TC 0 Z9 0 U1 0 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb316 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501438 ER PT J AU Guo, XG Chen, HL AF Guo, Xuguang Chen, Honglei TI Sweetened-beverages, coffee, and tea in relation to depression among older US adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Guo, Xuguang] Westat Corp, Durham, NC USA. [Guo, Xuguang; Chen, Honglei] NIEHS, Epidemiol Branch, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 616.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504074 ER PT J AU Gutkind, JS AF Gutkind, J. Silvio TI Novel G protein- and GPCR-Regulated Oncogenic Signaling Circuitries SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Gutkind, J. Silvio] NIDCR, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 338.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500537 ER PT J AU Guzman-Hernandez, ML Potter, G Kim, YJ Kiss, JZ Balla, T AF Guzman-Hernandez, Maria Luisa Potter, Gael Kim, Yeun Ju Kiss, Jozsef Z. Balla, Tamas TI The secretion of VEGF165 involves a shedding step from the cell surface SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Guzman-Hernandez, Maria Luisa; Kim, Yeun Ju; Balla, Tamas] NICHD, Sect Mol Signal Transduct Program Dev Neurosci, NIH, Bethesda, MD USA. [Potter, Gael; Kiss, Jozsef Z.] Univ Geneva, Dept Neurosci, Geneva, Switzerland. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 591.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501282 ER PT J AU Hammond, GR Balla, T AF Hammond, Gerry R. Balla, Tamas TI A new role for plasma membrane phosphatidylinositol 4-phosphate (PI4P)? SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hammond, Gerry R.; Balla, Tamas] NICHD, Program Dev Neurosci, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb84 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505458 ER PT J AU Hansen, JG Hootman, K Tang, W Brannon, PM Kritchevsky, S Houston, D Harris, T Garcia, M Liu, Y Lohman, K Cassano, PA AF Hansen, J. G. Hootman, K. Tang, W. Brannon, P. M. Kritchevsky, S. Houston, D. Harris, T. Garcia, M. Liu, Y. Lohman, K. Cassano, P. A. TI Predictors of Serum 25(OH) D in Elderly African-Americans in the Health, Aging, and Body Composition (Health ABC) Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hansen, J. G.; Hootman, K.; Tang, W.; Brannon, P. M.; Cassano, P. A.] Cornell Univ, Ithaca, NY USA. [Kritchevsky, S.; Houston, D.; Liu, Y.; Lohman, K.] Wake Forest Sch Med, Winston Salem, NC USA. [Harris, T.; Garcia, M.] NIA, Bethesda, MD 20892 USA. [Cassano, P. A.] Weill Cornell Med Coll, New York, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 245.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502442 ER PT J AU Hewitt, SM Perry, CD Ylaya, K Morris, J Chung, JY AF Hewitt, Stephen Merrill Perry, Candice D. Ylaya, Kris Morris, Jennifer Chung, Joon-Yong TI The Impact Of Different Additives To Neutral Buffered Formalin SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hewitt, Stephen Merrill; Perry, Candice D.; Ylaya, Kris; Morris, Jennifer; Chung, Joon-Yong] NCI, Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 52.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503008 ER PT J AU Hibbeln, JR Malde, M Osei-Hyiaman, D Lin, YH Pawlosky, R Madsen, L Kristiansen, K Froyland, L Avelheim, A AF Hibbeln, Joseph R. Malde, Marian Osei-Hyiaman, Douglas Lin, Yu Hong Pawlosky, Robert Madsen, Lise Kristiansen, Karsten Froyland, Livar Avelheim, Anita TI Dietary Linoleic Acid Elevates Endogenous Endocannabinoids (2-AG and Anandamide) and Induces Obesity SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hibbeln, Joseph R.; Lin, Yu Hong; Pawlosky, Robert] NIAAA, Sect Nutr Neurosci, Rockville, MD 20852 USA. [Malde, Marian; Madsen, Lise; Froyland, Livar; Avelheim, Anita] Natl Inst Nutr & Seafood Res NIFES, Bergen, Norway. [Osei-Hyiaman, Douglas] Nippon Boehringer Ingelheim Co Ltd, Kobe Pharma Res Inst, Dept Mol & Cellular Biol, Kobe, Hyogo, Japan. [Kristiansen, Karsten] Univ Copenhagen, Dept Biol, Copenhagen, Denmark. NR 0 TC 1 Z9 1 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 48.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500167 ER PT J AU Hiranita, T Paul, SL Tanda, G Katz, JL AF Hiranita, Takato Paul, Soto L. Tanda, Gianluigi Katz, Jonathan L. TI Specificity of cocaine-induced dopamine-independent sigma agonist self-administration SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hiranita, Takato; Tanda, Gianluigi; Katz, Jonathan L.] NIDA, Psychobiol Sect, IRP, NIH, Baltimore, MD USA. [Paul, Soto L.] Johns Hopkins Univ, Sch Med, Behav Biol Res Ctr, Baltimore, MD USA. RI Tanda, Gianluigi/B-3318-2009; Hiranita, Takato/G-6567-2011 OI Tanda, Gianluigi/0000-0001-9526-9878; NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 659.11 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502207 ER PT J AU Hosokawa, H Dip, PV Merkulova, M Bakulina, A Zhuang, ZJ Khatri, A Jian, XY Randazzo, PA Ausiello, DA Gruber, G Marshansky, V AF Hosokawa, Hiroyuki Dip, Phat V. Merkulova, Maria Bakulina, Anastasia Zhuang, Zhenjie Khatri, Ashok Jian, Xiaoying Randazzo, Paul A. Ausiello, Dennis A. Grueber, Gerhard Marshansky, Vladimir TI V-ATPase is a novel evolutionarily conserved cytohesin-signaling receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hosokawa, Hiroyuki; Merkulova, Maria; Zhuang, Zhenjie; Khatri, Ashok; Ausiello, Dennis A.; Marshansky, Vladimir] Harvard Univ, Sch Med, MGH, CSB,PMB, Boston, MA USA. [Dip, Phat V.; Grueber, Gerhard] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore. [Bakulina, Anastasia] State Res Ctr Virol Biotech, Koltsov, Russia. [Jian, Xiaoying; Randazzo, Paul A.] NCI, Lab Cel Mol Biol, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1031.23 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501466 ER PT J AU Houston, DK Tooze, JA Hausman, DB Johnson, MA Cauley, JA Bauer, DC Tylavsky, FA Harris, TB Kritchevsky, SB AF Houston, Denise K. Tooze, Janet A. Hausman, Dorothy B. Johnson, Mary Ann Cauley, Jane A. Bauer, Douglas C. Tylavsky, Frances A. Harris, Tamara B. Kritchevsky, Stephen B. TI 25-hydroxyvitamin D and adiposity in older black and white adults: the Health ABC study SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Houston, Denise K.; Tooze, Janet A.; Kritchevsky, Stephen B.] Wake Forest Sch Med, Winston Salem, NC USA. [Hausman, Dorothy B.; Johnson, Mary Ann] Univ Georgia, Athens, GA 30602 USA. [Cauley, Jane A.] Univ Pittsburgh, Pittsburgh, PA USA. [Bauer, Douglas C.] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Tylavsky, Frances A.] Univ Tennessee, Hlth Sci Ctr, Memphis, TN USA. [Harris, Tamara B.] NIA, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 245.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860501197 ER PT J AU Howden, R Cooley, I Van Dodewaard, C Arthur, S Cividanes, S McCann, K Gladwell, W Martin, J Scott, G Ray, M Mishina, Y AF Howden, Reuben Cooley, Ian Van Dodewaard, Caitlin Arthur, Susan Cividanes, Samantha McCann, Kelly Gladwell, Wes Martin, Jessica Scott, Gregory Ray, Manas Mishina, Yuji TI Heart rate, PR-interval and QT-interval responses to 24hrs hyperoxia in Bmp2 and Bmp4 heterozygous mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Howden, Reuben; Cooley, Ian; Van Dodewaard, Caitlin; Arthur, Susan; Cividanes, Samantha] UNC Charlotte, Dept Kinesiol, Lab Syst Physiol, Charlotte, NC USA. [McCann, Kelly; Gladwell, Wes; Martin, Jessica] NIEHS, Lab Resp Biol, Durham, NC USA. [McCann, Kelly; Scott, Gregory; Ray, Manas; Mishina, Yuji] NIEHS, Knockout Core, Durham, NC USA. [Mishina, Yuji] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb664 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860506077 ER PT J AU Hruby, A O'Donnell, CJ Jacques, PF Meigs, JB Wood, RJ McKeown, NM AF Hruby, Adela O'Donnell, Christopher J. Jacques, Paul F. Meigs, James B. Wood, Richard J. McKeown, Nicola M. TI Associations of magnesium intake with coronary artery calcification in the Framingham Heart Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Hruby, Adela; Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [O'Donnell, Christopher J.] Massachusetts Gen Hosp, Dept Med, Div Cardiovasc, Boston, MA 02114 USA. [O'Donnell, Christopher J.; Meigs, James B.] Harvard Univ, Sch Med, Boston, MA USA. [Meigs, James B.] Massachusetts Gen Hosp, Dept Med, Div Gen Med, Boston, MA 02114 USA. [O'Donnell, Christopher J.] NHLBI, Framingham Heart Study, Framingham, MA USA. [Wood, Richard J.] Univ Massachusetts, Dept Nutr, Amherst, MA 01003 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 622.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502472 ER PT J AU Huang, J Liu, S Wang, YS Thazhathveetil, AK Wang, WD Seidman, MM AF Huang, Jing Liu, Shuo Wang, Yinsheng Thazhathveetil, Arun Kalliat Wang, Weidong Seidman, Michael M. TI Single Molecular Analysis of the Encounter of Replication Forks with DNA Interstrand Crosslinks SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Huang, Jing; Seidman, Michael M.] NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. [Wang, Weidong] NIA, Genet Lab, NIH, Baltimore, MD 21224 USA. [Liu, Shuo; Wang, Yinsheng] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA. [Thazhathveetil, Arun Kalliat] Northwestern Univ, Dept Chem, Evanston, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 538.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503393 ER PT J AU Izumi, Y Li, JX Burg, MB Ferraris, JD AF Izumi, Yuichiro Li Jinxi Burg, Mauice B. Ferraris, Joan D. TI Peptide affinity chromatography identifies proteins that bind to N-terminus of NFAT5 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Izumi, Yuichiro; Li Jinxi; Burg, Mauice B.; Ferraris, Joan D.] NIH, Syst Biol Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 728.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500234 ER PT J AU Jovic, M Kean, M Gingras, AC Brill, J Balla, T AF Jovic, Marko Kean, Michelle Gingras, Anne-Claude Brill, Julie Balla, Tamas TI Endosomal sorting of VAMP3 is regulated by Phosphatidylinositol 4-kinase IIa SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Jovic, Marko; Balla, Tamas] NICHD, NIH, Toronto, ON, Canada. [Kean, Michelle; Gingras, Anne-Claude] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada. [Brill, Julie] Hosp Sick Children, Program Dev & Stem Cell Biol, Toronto, ON M5G 1X8, Canada. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1017.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501116 ER PT J AU Kaiser, MC Rajendran, P Dashwood, MW Levine, MA Michaels, A Frei, B Dashwood, RH AF Kaiser, Matthew Carl Rajendran, Praveen Dashwood, Mohaiza W. Levine, Mark A. Michaels, Alexander Frei, Balz Dashwood, Roderick H. TI An epigenetic perspective on pharmacologic ascorbate in colon cancer SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kaiser, Matthew Carl] Oregon State Univ, Corvallis, OR 97331 USA. [Rajendran, Praveen; Dashwood, Mohaiza W.; Michaels, Alexander; Frei, Balz; Dashwood, Roderick H.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. [Levine, Mark A.] NIDDK, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 248.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883504240 ER PT J AU Kang, JH Tsai-Morris, CH Hassan, SA Dufau, ML AF Kang, J-H Tsai-Morris, C. H. Hassan, S. A. Dufau, M. L. TI Human Prolactin Receptor: Impact of subdomain D1 of the short form variant for its inhibitory action on prolactin induced function of the long form of the receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kang, J-H; Tsai-Morris, C. H.; Dufau, M. L.] NICHD, Sect Mol Endocrinol, PDEGEN, NIH, Bethesda, MD USA. [Hassan, S. A.] NIH, Ctr Mol Modeling, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 602.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500229 ER PT J AU Kant, A Graubard, BI AF Kant, Ashima Graubard, Barry I. TI Plain water and total moisture intakes and subsequent risk of all-cause mortality in a national cohort SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kant, Ashima] CUNY, Queens Coll, Flushing, NY USA. [Graubard, Barry I.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 847.24 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505321 ER PT J AU Khan, FA Park, SJ Chung, JH Manganiello, VC AF Khan, Faiyaz Ahmad Park, Sung-Jun Chung, Jay H. Manganiello, Vincent C. TI Effects of SRT1720 (SRT) and Resveratrol (RSV) on AMP-activated protein kinase (AMPK) and PKA-signaling in Adipocytes: Roles for Phosphodiesterase (PDE) (3 and 4) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Khan, Faiyaz Ahmad; Manganiello, Vincent C.] NHLBI, Cardiovasc Pulm Branch, NIH, Bethesda, MD 20892 USA. [Park, Sung-Jun; Chung, Jay H.] NHLBI, Lab Obes & Aging Res, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 604.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503555 ER PT J AU Kim, SJ Cheresh, P Tularisam, S Schumacker, P Weitzman, SA Bohr, V Kamp, DW AF Kim, Seok-Jo Cheresh, Paul Tularisam, Sandhya Schumacker, Paul Weitzman, Sigmund A. Bohr, Vilhelm Kamp, David W. TI Mitochondria-Targeted Ogg1 and Aco-2 Prevent Oxidant-Induced Mitochondrial DNA Damage SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kim, Seok-Jo; Cheresh, Paul; Tularisam, Sandhya; Schumacker, Paul; Weitzman, Sigmund A.; Kamp, David W.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. [Bohr, Vilhelm] NIA, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 722.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502504 ER PT J AU Kirby, T AF Kirby, Thomas TI NMR Studies of the DNA Polymerase beta L22P Variant SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kirby, Thomas] NIEHS, LSB, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb77 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502341 ER PT J AU Kirkpatrick, SI Reedy, J Krebs-Smith, SM AF Kirkpatrick, Sharon I. Reedy, Jill Krebs-Smith, Susan M. TI Sources of dietary guidance-based food groups and empty calories among the US population in relation to income and race/ethnicity SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kirkpatrick, Sharon I.; Reedy, Jill; Krebs-Smith, Susan M.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 232.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501192 ER PT J AU Kirkpatrick, SI Thompson, FE Subar, AF Douglass, D Zimmerman, TP Kahle, LL George, SM Potischman, N AF Kirkpatrick, Sharon I. Thompson, Frances E. Subar, Amy F. Douglass, Deirdre Zimmerman, Thea P. Kahle, Lisa L. George, Stephanie M. Potischman, Nancy TI Validity of the National Cancer Institute's Automated Self-Administered 24-hour Recall (ASA24): Results of a Feeding Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kirkpatrick, Sharon I.; Thompson, Frances E.; Subar, Amy F.; George, Stephanie M.; Potischman, Nancy] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Douglass, Deirdre; Zimmerman, Thea P.] WESTAT Corp, Rockville, MD 20850 USA. [Kahle, Lisa L.] Informat Management Serv Inc, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 230.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505066 ER PT J AU Kirkpatrick, SI Guenther, PM Dodd, KW AF Kirkpatrick, Sharon I. Guenther, Patricia M. Dodd, Kevin W. TI The effects of recall sequence/mode and day of week on the estimation of usual intake from 24-hour recall data: An analysis of the 2003-2004 National Health and Nutrition Examination Survey SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kirkpatrick, Sharon I.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Dodd, Kevin W.] NCI, Canc Prevent Div, Bethesda, MD 20892 USA. [Guenther, Patricia M.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 621.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504076 ER PT J AU Kochukov, MY Abramowitz, J Birnbaumer, L Marrelli, SP AF Kochukov, Mikhail Y. Abramowitz, Joel Birnbaumer, Lutz Marrelli, Sean P. TI Sustained ATP-mediated vasodilation of cerebral arteries requires Ca2+influx via TRPC3 channels to produce endothelial SKCa channel activation and hyperpolarization SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kochukov, Mikhail Y.; Marrelli, Sean P.] Baylor Coll Med, Houston, TX 77030 USA. [Abramowitz, Joel; Birnbaumer, Lutz] Inst Environm Hlth Sci, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 925.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860506105 ER PT J AU Kraft, ML Klitzing, HA Frisz, JF Kou, KY Weber, PK Zimmerberg, J AF Kraft, Mary L. Klitzing, Haley A. Frisz, Jessica F. Kou, Kaiyan Weber, Peter K. Zimmerberg, Joshua TI Mechanisms of non-random sphingolipid organization in the plasma membranes of fibroblast cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kraft, Mary L.; Klitzing, Haley A.; Frisz, Jessica F.; Kou, Kaiyan] Univ Illinois, Sch Chem Sci, Urbana, IL 61801 USA. [Weber, Peter K.] Lawrence Livermore Natl Lab, Livermore, CA USA. [Zimmerberg, Joshua] NICHHD, Program Phys Biol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1023.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501121 ER PT J AU Kranz, S Dodd, K Juan, WY Johnson, LK Jahns, L AF Kranz, Sibylle Dodd, Kevin Juan, WenYen Johnson, Luann K. Jahns, Lisa TI Essential steps in the analysis of NHANES dietary data SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kranz, Sibylle] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Dodd, Kevin] NCI, Bethesda, MD 20892 USA. [Juan, WenYen] US FDA, College Pk, MD USA. [Johnson, Luann K.; Jahns, Lisa] USDA, Grand Forks, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 848.20 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505363 ER PT J AU Kranz, S Dodd, KW Juan, WY Johnson, LK AF Kranz, Sibylle Dodd, Kevin W. Juan, WenYen Johnson, LuAnn K. TI Comparison of main contributors to dietary fiber and whole grain in Americans' Diet: NHANES 2003-2010 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kranz, Sibylle] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Dodd, Kevin W.] NCI, Rockville, MD USA. [Juan, WenYen] US FDA, College Pk, MD USA. [Johnson, LuAnn K.] USDA, Grand Forks, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1065.11 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502609 ER PT J AU Kris-Etherton, PM Akabas, S Pratt, C Saltzman, E Krebs, N Levy, M AF Kris-Etherton, Penny M. Akabas, Sharon Pratt, Charlotte Saltzman, Edward Krebs, Nancy Levy, Matthew TI Future Directions for Implementing Nutrition Across the Continuum of Medical and Health Professions Education and Training, and Research SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kris-Etherton, Penny M.] Penn State Univ, University Pk, PA 16802 USA. [Akabas, Sharon] Columbia Univ, Inst Human Nutr, New York, NY 10032 USA. [Pratt, Charlotte] NHLBI, Bethesda, MD 20892 USA. [Saltzman, Edward] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Krebs, Nancy] Univ Colorado, Aurora, CO USA. [Levy, Matthew] Medstar Georgetown Univ Hosp, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 47.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502425 ER PT J AU Kristal, B Hubbard, VS Starke-Reed, P Bird, SS Marur, VR Sniatynski, M Sheldon, D AF Kristal, Bruce Hubbard, Van S. Starke-Reed, Pamela Bird, Susan S. Marur, Vasant R. Sniatynski, Matt Sheldon, Diane TI Calorie intake and blood lipidomics SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Kristal, Bruce; Bird, Susan S.; Marur, Vasant R.; Sniatynski, Matt; Sheldon, Diane] Harvard Univ, Sch Med, Boston, MA USA. [Hubbard, Van S.; Starke-Reed, Pamela] NIH, Div Nutr Res Coordinat, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1073.9 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503502 ER PT J AU Lazrak, A Yu, ZH Komarova, S Doran, SF Garantziotis, S Matalon, S AF Lazrak, Ahmed Yu, Zhihong Komarova, Svetlana Doran, Stephen F. Garantziotis, Stavros Matalon, Sadis TI Molecular mechanism of chlorine induced lung hyperreactivity in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Lazrak, Ahmed; Yu, Zhihong; Komarova, Svetlana; Doran, Stephen F.; Matalon, Sadis] Univ Alabama Birmingham, Pulm Injury Repair Ctr, Birmingham, AL USA. [Garantziotis, Stavros] NIEHS, Res Triangle Pk, NC 27709 USA. RI Garantziotis, Stavros/A-6903-2009 OI Garantziotis, Stavros/0000-0003-4007-375X NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1216.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500116 ER PT J AU Li, CC Le, K Moss, J Vaughan, M AF Li, Chun-Chun Le, Kang Moss, Joel Vaughan, Martha TI Interactions between Arf guanine nucleotide-exchange factors, BIG1 and BIG2 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Li, Chun-Chun; Le, Kang; Moss, Joel; Vaughan, Martha] NHLBI, Cardiovasc & Pulm Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 591.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500619 ER PT J AU Li, QL Anderson, VE Brunengraber, H Zhang, GF AF Li, Qingling Anderson, Vernon E. Brunengraber, Henri Zhang, Guo-Fang TI Multiple cycles of acetyl-CoA enolization catalyzed by citrate synthase (CS) and ATP-citrate lyase (ACL) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Li, Qingling; Brunengraber, Henri; Zhang, Guo-Fang] Case Western Reserve Univ, Cleveland, OH 44106 USA. [Anderson, Vernon E.] NIGMS, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 794.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501662 ER PT J AU Lippincott-Schwartz, JA AF Lippincott-Schwartz, Jennifer Anne TI Molecular dynamics of endomembrane structure and function SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Lippincott-Schwartz, Jennifer Anne] Eugene Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 336.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502156 ER PT J AU Liu, G Claxton, JS Sandoval, PC Chou, CL Knepper, MA AF Liu, Gary Claxton, J'Neka S. Sandoval, Pablo C. Chou, Chung-Lin Knepper, Mark A. TI Mass spectrometry-based pseudo-western blots SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Liu, Gary; Claxton, J'Neka S.; Sandoval, Pablo C.; Chou, Chung-Lin; Knepper, Mark A.] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1217.27 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505605 ER PT J AU Liu, HL Joshi-Barve, S Barve, S McClain, C Ramsden, C Kirpich, I AF Liu, Huilin Joshi-Barve, Swati Barve, Shirish McClain, Craig Ramsden, Christopher Kirpich, Irina TI Effects of Ethanol and Oxidized Metabolites of Linoleic Acid on Caco-2 cell Model of Intestinal Epithelial Barrier SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Liu, Huilin; Joshi-Barve, Swati; Barve, Shirish; McClain, Craig; Kirpich, Irina] Univ Louisville, Louisville, KY 40292 USA. [McClain, Craig] Louisville VAMC, Louisville, KY USA. [Ramsden, Christopher] NIAAA, Bethesda, MD 90034 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 890.20 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505099 ER PT J AU Liu, TY Bian, X Sun, S Hu, XY Klemm, RW Prinz, WA Rapoport, TA Hu, JJ AF Liu, Tina Yu Bian, Xin Sun, Sha Hu, Xiaoyu Klemm, Robin W. Prinz, William A. Rapoport, Tom A. Hu, Junjie TI The role of the C-terminus and transmembrane segments in facilitating atlastin-mediated endoplasmic reticulum fusion SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Liu, Tina Yu; Klemm, Robin W.; Rapoport, Tom A.] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA USA. [Liu, Tina Yu; Klemm, Robin W.; Rapoport, Tom A.] Howard Hughes Med Inst, Boston, MA 02115 USA. [Bian, Xin; Sun, Sha; Hu, Xiaoyu; Hu, Junjie] Nankai Univ, Dept Genet & Cell Biol, Coll Life Sci, Tianjin 300071, Peoples R China. [Bian, Xin; Sun, Sha; Hu, Xiaoyu; Hu, Junjie] Tianjin Key Lab Prot Sci, Tianjin, Peoples R China. [Prinz, William A.] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1016.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500427 ER PT J AU Locatelli-Hoops, SC Gorshkova, I Gawrisch, K Yeliseev, AA AF Locatelli-Hoops, Silvia Cristina Gorshkova, Inna Gawrisch, Klaus Yeliseev, Alexei A. TI Purification and Analysis of Rho-1D4-tagged Peripheral Cannabinoid Receptor CB2 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Locatelli-Hoops, Silvia Cristina; Gawrisch, Klaus; Yeliseev, Alexei A.] NIAAA, NIH, Bethesda, MD 90034 USA. [Gorshkova, Inna] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 790.18 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501005 ER PT J AU Lyons, CC Yellayi, S Cann, J Hart, B Postnikova, E AF Lyons, Cassandra Corine Yellayi, Sri Cann, Jennifer Hart, Brit Postnikova, Elena TI Stabilization and Direct Detection of Green Fluorescent Proteins in Histological Preparations for Pathological Evaluation of Infectious Disease SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Lyons, Cassandra Corine; Yellayi, Sri; Cann, Jennifer; Hart, Brit; Postnikova, Elena] NIAID, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb469 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500403 ER PT J AU Ma, JT Sloan, M Fox, CS Hoffmann, U Jacques, PF McKeown, NM AF Ma, Jiantao Sloan, Matthew Fox, Caroline S. Hoffmann, Udo Jacques, Paul F. McKeown, Nicola M. TI Sugar-sweetened beverage consumption is associated with relative distribution of abdominal adipose tissue in the Framingham Heart Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Ma, Jiantao] Tufts Univ, Jean Mayer USDA HNRCA, Boston, MA 02111 USA. [Sloan, Matthew] Univ Massachusetts, Sch Med, Worcester, MA USA. [Fox, Caroline S.] NHLBI, Framingham, MA USA. [Fox, Caroline S.] Harvard Univ, Sch Med, Boston, MA USA. [Hoffmann, Udo] Massachusetts Gen Hosp, Dept Radiol, Boston, MA 02114 USA. [Jacques, Paul F.; McKeown, Nicola M.] Tutfs Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 126.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860500120 ER PT J AU Marshansky, V Hosokawa, H Zhuang, ZJ Randazzo, PA Gruber, G Ausiello, DA AF Marshansky, Vladimir Hosokawa, Hiroyuki Zhuang, Zhenjie Randazzo, Paul A. Grueber, Gerhard Ausiello, Dennis A. TI Novel role of cytohesin-2 in regulation of macropinocytosis pathway and cell proliferation SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Marshansky, Vladimir; Hosokawa, Hiroyuki; Zhuang, Zhenjie; Ausiello, Dennis A.] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Program Membrane Biol,Ctr Syst Biol, Boston, MA USA. [Randazzo, Paul A.] NCI, Lab Cel Mol Biol, Ctr Canc Res, Bethesda, MD 20892 USA. [Grueber, Gerhard] Nanyang Technol Univ, Sch Biol Sci, Div Struct Biol Biochem, Singapore, Singapore. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 591.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500220 ER PT J AU Marzec, JM Ciencewicki, JM Kleeberger, SR AF Marzec, Jacqui M. Ciencewicki, Jon M. Kleeberger, Steven R. TI Functional variants of MARCO modulate susceptibility to respiratory syncytial virus (RSV) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Marzec, Jacqui M.; Ciencewicki, Jon M.; Kleeberger, Steven R.] NIEHS, Lab Resp Biol, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 550.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502442 ER PT J AU Merluzzi, AP Hurwitz, Z Briscione, M Cobuzzi, J Wetzell, B Rice, K Riley, A AF Merluzzi, Andrew Paul Hurwitz, Zachary Briscione, Maria Cobuzzi, Jennifer Wetzell, Bradley Rice, Kenner Riley, Anthony TI Differential Expression of MDPV-Induced Taste Aversions and Thermoregulation in Adolescent and Adult Rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Merluzzi, Andrew Paul; Hurwitz, Zachary; Briscione, Maria; Cobuzzi, Jennifer; Wetzell, Bradley; Riley, Anthony] American Univ, Washington, DC 20016 USA. [Rice, Kenner] NIDA, Chem Biol Res Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1098.17 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504188 ER PT J AU Mikelis, CM Simaan, M Li, WL Szabo, R Martin, D Mukouyama, Y Bugge, TH Gutkind, SJ AF Mikelis, Constantinos Marios Simaan, May Li, Wenling Szabo, Roman Martin, Daniel Mukouyama, Yosuke Bugge, Thomas H. Gutkind, Silvio J. TI PDZ-RhoGEF and LARG are essential for embryo development, and provide a link between thrombin receptors and Rho activation SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Mikelis, Constantinos Marios; Simaan, May; Szabo, Roman; Martin, Daniel; Bugge, Thomas H.; Gutkind, Silvio J.] NIDCR, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD USA. [Li, Wenling; Mukouyama, Yosuke] NHLBI, Lab Stem Cell & Neurovasc Biol, Genet & Dev Biol Ctr, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1046.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883502360 ER PT J AU Miller, DS Cannon, RE Peart, J Campos, CR Smith, LK Wang, XQ AF Miller, David S. Cannon, Ronald E. Peart, John Campos, Christopher R. Smith, Lindsay K. Wang, Xueqian TI Sulfuraphane (SFN) activation of nuclear factor E2-related factor 2 (Nrf2) increases expression and transport activity of P-glycoprotein (P-gp) and breast cancer related protein (Bcrp) at the blood-brain barrier (BBB) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Miller, David S.; Cannon, Ronald E.; Peart, John; Campos, Christopher R.; Smith, Lindsay K.; Wang, Xueqian] NIEHS, Lab Toxicol & Pharmacol, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 891.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502010 ER PT J AU Miranda, CA Lee, JW Chou, CL Knepper, MA AF Miranda, C. A. Lee, J. W. Chou, C. L. Knepper, M. A. TI AQP2 poly-phosphorylation and water permeability in inner medullary collecting duct (IMCD) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Miranda, C. A.; Lee, J. W.; Chou, C. L.; Knepper, M. A.] NHLBI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1148.18 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503252 ER PT J AU Moaddel, R Singh, N Ravichandran, S Norton, DD Fugmann, S AF Moaddel, Ruin Singh, Nagendra Ravichandran, Sarangan Norton, Darrell D. Fugmann, Sebastian TI The identification of novel modulators for the SIRT6 protein SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Moaddel, Ruin; Singh, Nagendra] NIA, Clin Invest Lab, NIH, Baltimore, MD 21224 USA. [Norton, Darrell D.] NIA, LMBI, NIH, Baltimore, MD 21224 USA. [Ravichandran, Sarangan] FNLCR, Adv Biomed Comp Ctr, Frederick, MD USA. [Fugmann, Sebastian] LMBI, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 890.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505412 ER PT J AU Moritz, AE Free, RB Conroy, J Doyle, T Southall, N Ferrer, M Donthamsetti, P Javitch, JA Sibley, DR AF Moritz, Amy E. Free, R. Benjamin Conroy, Jennie Doyle, Trevor Southall, Noel Ferrer, Marc Donthamsetti, Prashant Javitch, Jonathan A. Sibley, David R. TI Identification of a novel dopaminergic agonist that selectively activates the D3 dopamine receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Moritz, Amy E.; Free, R. Benjamin; Conroy, Jennie; Doyle, Trevor; Sibley, David R.] NINDS, MNS, NIH, Bethesda, MD 20892 USA. [Southall, Noel; Ferrer, Marc] NIH, Natl Ctr Adv Translat Sci, Rockville, MD USA. [Donthamsetti, Prashant; Javitch, Jonathan A.] Columbia Univ, Coll Phys & Surg, Ctr Mol Recognit, New York, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 655.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503518 ER PT J AU Nader, S Porrino, L Miller, M Calhoun, T Martelle, S Banala, A Newman, A Nader, M AF Nader, Susan Porrino, Linda Miller, Mack Calhoun, Tonya Martelle, Susan Banala, Ashwini Newman, Amy Nader, Michael TI Effects of chronic treatment with the D3 receptor-selective compound PG619 on cocaine (COC) self-administration and FDG brain activity in rhesus monkeys SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Nader, Susan; Porrino, Linda; Miller, Mack; Calhoun, Tonya; Martelle, Susan; Nader, Michael] Wake Forest Sch Med, Winston Salem, NC USA. [Banala, Ashwini; Newman, Amy] NIDA IRP, Med Chem Sect, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 659.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502470 ER PT J AU Namaste, S Suchdev, P Aaron, G AF Namaste, Sorrel Suchdev, Parminder Aaron, Grant TI Biomarkers Reflecting Inflammation and Nutrition Determinants of Anemia (BRINDA): Project Overview SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Namaste, Sorrel] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Pediat Growth & Nutr Branch, NIH, Bethesda, MD USA. [Suchdev, Parminder] Ctr Dis Control & Prevent, Nutr Branch, Atlanta, GA USA. [Aaron, Grant] Global Alliance Improved Nutr, Geneva, Switzerland. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA lb270 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505565 ER PT J AU Narlikar, G Canzio, D Liao, MF Naber, N Pate, E Larson, A Wu, SP Cooke, R Schuck, P Cheng, YF AF Narlikar, Geeta Canzio, Daniele Liao, Maofu Naber, Nariman Pate, Ed Larson, Adam Wu, Shenping Cooke, Roger Schuck, Peter Cheng, Yifan TI Mechanistic Analysis of HP1 heterochromatin assembly SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Narlikar, Geeta; Canzio, Daniele; Liao, Maofu; Naber, Nariman; Pate, Ed; Larson, Adam; Wu, Shenping; Cooke, Roger; Cheng, Yifan] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Schuck, Peter] NIBIB, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 456.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500560 ER PT J AU Nayeem, MA Pradhan, I Mustafa, SJ Morisseau, C Falck, JR Zeldin, DC AF Nayeem, Mohammed A. Pradhan, Isha Mustafa, S. Jamal Morisseau, Christophe Falck, John R. Zeldin, Darryl C. TI Adenosine A2A receptor modulates vascular response in soluble epoxide hydrolase-null mice through cyp2j-epoxygenases and PPAR(gamma) SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Nayeem, Mohammed A.; Pradhan, Isha; Mustafa, S. Jamal] W Virginia Univ, CCRS, Morgantown, WV 26506 USA. [Morisseau, Christophe] Univ Calif Davis, UCD Canc Ctr, Davis, CA 95616 USA. [Falck, John R.] UTSWMC, Dallas, TX USA. [Zeldin, Darryl C.] NIEHS, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1090.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502613 ER PT J AU Nicastro, HL Mondul, AM Rohrmann, S Platz, EA AF Nicastro, Holly L. Mondul, Alison M. Rohrmann, Sabine Platz, Elizabeth A. TI Associations between urinary soy isoflavones and inflammatory markers in adults in the United States in 2005-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Nicastro, Holly L.; Mondul, Alison M.] NCI, Rockville, MD USA. [Rohrmann, Sabine] Univ Zurich, Zurich, Switzerland. [Platz, Elizabeth A.] Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1059.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501140 ER PT J AU Nicastro, HL Clevidence, BA Charron, CS Gebauer, SK Dawson, HD Cooke, MS Mistry, V Singh, R Milner, JA Novotny, JA AF Nicastro, Holly L. Clevidence, Beverly A. Charron, Craig S. Gebauer, Sarah K. Dawson, Harry D. Cooke, Marcus S. Mistry, Vilas Singh, Raj Milner, John A. Novotny, Janet A. TI Blackberries decrease DNA damage after 3 h, but not after 6 d, in healthy adult volunteers SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Nicastro, Holly L.] NCI, Nutr Sci Res Grp, Rockville, MD USA. [Clevidence, Beverly A.; Charron, Craig S.; Gebauer, Sarah K.; Dawson, Harry D.; Milner, John A.; Novotny, Janet A.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Cooke, Marcus S.; Mistry, Vilas; Singh, Raj] Univ Leicester, Dept Canc Studies, Leicester, Leics, England. RI Dawson, Harry/H-8242-2013 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 864.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501042 ER PT J AU Noinaj, N Kuszak, A Gumbart, JC Lukacik, P Chang, HS Easley, N Lithgow, T Buchanan, SK AF Noinaj, Nicholas Kuszak, Adam Gumbart, J. C. Lukacik, Petra Chang, Hoshing Easley, Nicole Lithgow, Trevor Buchanan, Susan K. TI Structural insights into the role of BamA in the biogenesis of beta-barrel membrane proteins in Gram-negative bacteria SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP Amer Soc Pharmacol & Expt Therapeut (ASPET), British Pharmacol Soc (BPS), Amer Assoc Anatomists (AAA), Amer Physiol Soc (APS), Amer Soc Biochem & Mol Biol (ASBMB), Amer Soc Investigat Pathol (ASIP), Amer Soc Nutr (ASN) C1 [Noinaj, Nicholas; Kuszak, Adam; Easley, Nicole; Buchanan, Susan K.] NIDDK, NIH, Bethesda, MD 20857 USA. [Gumbart, J. C.] Georgia Inst Technol, Atlanta, GA USA. [Lukacik, Petra] Diamond Light Source Ltd, Didcot, Oxon, England. [Chang, Hoshing] US FDA, Rockville, MD USA. [Lithgow, Trevor] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia. NR 0 TC 1 Z9 1 U1 0 U2 9 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1025.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500435 ER PT J AU Nostramo, R Tillinger, A Saavedra, JM Serova, L Kumar, A Pandey, V Kvetnansky, R Sabban, EL AF Nostramo, Regina Tillinger, Andrej Saavedra, Juan M. Serova, Lidia Kumar, Ashok Pandey, Varunkumar Kvetnansky, Richard Sabban, Esther L. TI Stress-triggered regulation of the adrenomedullary angiotensin II type 2 receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Nostramo, Regina; Tillinger, Andrej; Serova, Lidia; Kumar, Ashok; Pandey, Varunkumar; Sabban, Esther L.] New York Med Coll, Valhalla, NY 10595 USA. [Saavedra, Juan M.] NIH, Pharmacol Sect, Bethesda, MD 20892 USA. [Kumar, Ashok; Pandey, Varunkumar] Univ Toledo, Coll Med, Toledo, OH 43606 USA. [Kvetnansky, Richard] Slovak Acad Sci, Inst Expt Endocrinol, Bratislava, Slovakia. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 936.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860506008 ER PT J AU Oubrahim, H Shi, YL Chock, PB AF Oubrahim, Hammou Shi, Yilan Chock, P. Boon TI Pasteurella multocida toxin (PMT) activates mTOR and inhibits PP2A via G alpha q/11/PLC beta/PKC SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Oubrahim, Hammou] NHLBI, Biochem Lab, NIH, Bethesda, MD 20892 USA. [Shi, Yilan; Chock, P. Boon] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1038.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883503153 ER PT J AU Pacher, P Mukhopadhyay, P Rajesh, M Horvath, B Batkai, S Park, O Hasko, G Liaudet, L Wink, D Mechoulam, R AF Pacher, Pal Mukhopadhyay, Partha Rajesh, Mohanraj Horvath, Bela Batkai, Sandor Park, Ogyi Hasko, Gyoergy Liaudet, Lucas Wink, David Mechoulam, Raphael TI Cannabidiol protects against hepatic ischemia/reperfusion injury by attenuating inflammatory signaling and response, oxidative/nitrative stress, and cell death SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pacher, Pal; Mukhopadhyay, Partha; Rajesh, Mohanraj; Horvath, Bela; Batkai, Sandor; Park, Ogyi] NIAAA, NIH, Rockville, MD 20852 USA. [Hasko, Gyoergy] UMDNJ New Jersey Med Sch, Newark, NJ USA. [Liaudet, Lucas] Univ Lausanne Hosp, Lausanne, Switzerland. [Wink, David] NCI, NIH, Bethesda, MD 20892 USA. [Mechoulam, Raphael] Hebrew Univ Jerusalem, Jerusalem, Israel. RI Pacher, Pal/B-6378-2008; Horvath, Bela/A-7368-2009; Batkai, Sandor/H-7983-2014; Liaudet, Lucas/E-1322-2017 OI Pacher, Pal/0000-0001-7036-8108; Liaudet, Lucas/0000-0003-2670-4930 NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 890.17 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860505309 ER PT J AU Pacher, P Mukhopadhyay, P Horvath, B Zsengeller, Z Batkai, S Cao, ZX Kechrid, M Holovac, E Erdelyi, K Liaudet, L Stillman, IE Joseph, J Kalyanaraman, B AF Pacher, Pal Mukhopadhyay, Partha Horvath, Bela Zsengeller, Zsuzsanna Batkai, Sandor Cao, Zongxian Kechrid, Malek Holovac, Eileen Erdelyi, Katalin Liaudet, Lucas Stillman, Isaac E. Joseph, Joy Kalyanaraman, Balaraman TI Mitochondrial reactive oxygen species generation triggers inflammatory response and tissue injury associated with hepatic ischemia-reperfusion: Therapeutic potential of mitochondrially targeted antioxidants SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pacher, Pal; Mukhopadhyay, Partha; Horvath, Bela; Batkai, Sandor; Cao, Zongxian; Kechrid, Malek; Holovac, Eileen; Erdelyi, Katalin] NIAAA, NIH, Rockville, MD 20852 USA. [Zsengeller, Zsuzsanna; Stillman, Isaac E.] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA. [Liaudet, Lucas] Univ Lausanne Hosp, Lausanne, Switzerland. [Joseph, Joy; Kalyanaraman, Balaraman] Med Coll Wisconsin, Milwaukee, WI 53226 USA. RI Horvath, Bela/A-7368-2009; Batkai, Sandor/H-7983-2014; Liaudet, Lucas/E-1322-2017 OI Liaudet, Lucas/0000-0003-2670-4930 NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 650.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504615 ER PT J AU Pacher, P Mukhopadhyay, P Horvath, B Zsengeller, Z Zielonka, J Kechrid, M Stillman, IE Parikh, SM Joseph, J Kalyanaraman, B AF Pacher, Pal Mukhopadhyay, Partha Horvath, Bela Zsengeller, Zsuzsanna Zielonka, Jacek Kechrid, Malek Stillman, Isaac E. Parikh, Samir M. Joseph, Joy Kalyanaraman, Balaraman TI Mitochondrial-targeted antioxidants represent a promising approach for prevention of cisplatin-induced nephropathy SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pacher, Pal; Mukhopadhyay, Partha; Horvath, Bela; Kechrid, Malek] NIAAA, NIH, Rockville, MD 20852 USA. [Zsengeller, Zsuzsanna; Stillman, Isaac E.; Parikh, Samir M.] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA. [Zsengeller, Zsuzsanna; Stillman, Isaac E.; Parikh, Samir M.] Harvard Univ, Sch Med, Boston, MA 02215 USA. [Zielonka, Jacek; Joseph, Joy; Kalyanaraman, Balaraman] Med Coll Wisconsin, Milwaukee, WI 53226 USA. RI Horvath, Bela/A-7368-2009; Zielonka, Jacek/N-9546-2014 OI Zielonka, Jacek/0000-0002-2524-0145 NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 889.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504130 ER PT J AU Pacher, P Horvath, B Mukhopadhyay, P Wink, D Gertsch, J AF Pacher, Pal Horvath, Bela Mukhopadhyay, Partha Wink, David Gertsch, Juerg TI beta-Caryophyllene ameliorates cisplatin-induced nephrotoxicity in a cannabinoid 2 receptor-dependent manner SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pacher, Pal; Horvath, Bela; Mukhopadhyay, Partha] NIAAA, NIH, Rockville, MD 20852 USA. [Wink, David] NCI, NIH, Bethesda, MD 20892 USA. [Gertsch, Juerg] Univ Bern, Bern, Switzerland. RI Horvath, Bela/A-7368-2009 NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 704.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860503107 ER PT J AU Pang, ALY Title, AC Rennert, OM AF Pang, Alan L. Y. Title, Alexandra C. Rennert, Owen M. TI Expression of the mouse Lin28a gene is epigenetically regulated by histone modification SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pang, Alan L. Y.; Title, Alexandra C.; Rennert, Owen M.] NICHD, LCG, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 550.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883501255 ER PT J AU Patel, S Cobb, P Saydah, S Zhang, XP De Jesus, J Cogswell, M AF Patel, Sheena Cobb, Paul Saydah, Sharon Zhang, Xuanping De Jesus, Janet Cogswell, Mary TI A systematic review of the effects of sodium reduction on glucose levels SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Patel, Sheena; Cobb, Paul; Cogswell, Mary] Ctr Dis Control & Prevent, Div Heart Dis & Stroke Prevent, Atlanta, GA USA. [Zhang, Xuanping] Ctr Dis Control & Prevent, Div Diabet Translat, Atlanta, GA USA. [Patel, Sheena] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Saydah, Sharon] Ctr Dis Control & Prevent, Div Diabet Translat, Hyattsville, MD USA. [De Jesus, Janet] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 622.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883505502 ER PT J AU Pathan, AR Fields, B Birnbaumer, L Zheng, F Rusch, NJ AF Pathan, Asif Rahimkhan Fields, Brandi Birnbaumer, Lutz Zheng, Fang Rusch, Nancy J. TI Genetic deletion of the TRPC3 channel blunts the development of angiotensin II-induced hypertension in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pathan, Asif Rahimkhan; Fields, Brandi; Zheng, Fang; Rusch, Nancy J.] Univ Arkansas Med Sci, Little Rock, AR 72205 USA. [Birnbaumer, Lutz] NIEHS, Neurobiol Lab, Div Intramural Res, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 654.18 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860504248 ER PT J AU Popuri, V Huang, J Mahesh, R Tadokoro, T Deborah, CL Bohr, VA AF Popuri, Venkateswarlu Huang, Jing Mahesh, Ramamoorthy Tadokoro, Takashi Deborah, Croteau L. Bohr, Vilhelm A. TI RECQL5 plays both co-operative and complementary roles with WRN syndrome helicase SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Popuri, Venkateswarlu; Huang, Jing; Mahesh, Ramamoorthy; Tadokoro, Takashi; Deborah, Croteau L.; Bohr, Vilhelm A.] NIA, LMG, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 542.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 157GG UT WOS:000319883500174 ER PT J AU Pradhan, I Mustafa, JS Zeldin, DC Ledent, C Falck, JR Nayeem, MA AF Pradhan, Isha Mustafa, Jamal S. Zeldin, Darryl C. Ledent, Catherine Falck, John R. Nayeem, Mohammed A. TI Modulation of vascular response by high salt intake depends on the presence or absence of adenosine A2A receptor using A2A AR-null mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB) CY APR 20-24, 2013 CL Boston, MA SP ASPET, British Pharmacol Soc (BPS) C1 [Pradhan, Isha; Mustafa, Jamal S.; Nayeem, Mohammed A.] W Virginia Univ, Morgantown, WV 26506 USA. [Zeldin, Darryl C.] NIEHS, Div Intramural Res, NIH, Res Triangle Pk, NC 27709 USA. [Ledent, Catherine] Univ Libre Brussels, Brussels, Belgium. [Falck, John R.] Univ Texas South Western, Dallas, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2013 VL 27 MA 1092.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 156YK UT WOS:000319860502615 ER EF