TY - JOUR T1 - Carcinogenesis by nitrosohydroxyethylurea and nitrosomethoxyethylurea in F344 rats. AN - 78207217; 3130352 AB - Nitroso-2-hydroxyethylurea and its methyl ether, nitroso-2-methoxyethylurea, were administered to male and female rats by gavage, each at two dose rates. The highest dose rate of nitrosohydroxyethylurea was 14 mg twice a week for 18 weeks, which led to death of all animals by week 34 with a variety of neoplasms, which have been observed in earlier experiments at lower doses, and included those in lung, colon, thyroid, forestomach, tongue, duodenum, jejunum, Zymbal's gland, thymus and mammary gland adenocarcinomas. At a dose 10 times smaller, the animals survived much longer, but the distribution of tumors was similar, except that there were few of the duodenum and jejunum. At an equimolar dose of nitrosomethoxyethylurea (1.6 mg, twice a week), the pattern of tumors was similar to that seen with nitrosohydroxyethylurea, including the absence of tumors in the duodenum and jejunum, although there were tumors of the colon. The rats treated with the methyl ether died earlier than those given nitrosohydroxyethylurea, indicating a greater potency of the former. At a dose of 3.2 mg twice a week, nitrosomethoxyethylurea produced the same pattern of tumors as the lower dose, but the animals died earlier. In all groups, there were more lung tumors in males than in females, and this was true to a lesser extent of the colon. The main effect of methylation of the hydroxyl group in nitrosohydroxyethylurea was to increase the potency of the carcinogen, but there was no effect on the target organ specificity of the nitrosourea. JF - Japanese journal of cancer research : Gann AU - Lijinsky, W AU - Kovatch, R M AD - Laboratory of Chemical and Physical Carcinogenesis, NCI-Frederick Cancer Research Facility, MD 21701. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 181 EP - 186 VL - 79 IS - 2 SN - 0910-5050, 0910-5050 KW - Carcinogens KW - 0 KW - 1-(2-methyoxyethyl)-1-nitrosourea KW - 108278-70-2 KW - 1-(2-hydroxyethyl)-1-nitrosourea KW - 13743-07-2 KW - DNA KW - 9007-49-2 KW - Ethylnitrosourea KW - P8M1T4190R KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Stomach Neoplasms -- chemically induced KW - Sex Factors KW - DNA -- metabolism KW - Male KW - Female KW - Structure-Activity Relationship KW - Ethylnitrosourea -- toxicity KW - Ethylnitrosourea -- metabolism KW - Neoplasms, Experimental -- chemically induced KW - Ethylnitrosourea -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78207217?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Japanese+journal+of+cancer+research+%3A+Gann&rft.atitle=Carcinogenesis+by+nitrosohydroxyethylurea+and+nitrosomethoxyethylurea+in+F344+rats.&rft.au=Lijinsky%2C+W%3BKovatch%2C+R+M&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-02-01&rft.volume=79&rft.issue=2&rft.spage=181&rft.isbn=&rft.btitle=&rft.title=Japanese+journal+of+cancer+research+%3A+Gann&rft.issn=09105050&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-14 N1 - Date created - 1988-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - H-ras p21 and peanut lectin immunoreactivity of hyperplastic, preneoplastic and neoplastic urinary bladder lesions in rats. AN - 78203548; 3130347 AB - Hyperplastic, preneoplastic and neoplastic urinary bladder lesions induced by bladder carcinogens and toxins in the rat were evaluated for immunoreactivity with polyclonal or monoclonal antibodies to H-ras p21 or binding to peanut lectin with avidin-biotin immunocytochemistry. A low proportion (less than 20%) of hyperplastic and neoplastic bladder lesions induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine and fixed in Bouin's fixative only were immunoreactive on the cell membrane with the antibodies to H-ras p21. Lectin binding was found for these lesions, as well, even in formalin-fixed tissue and for lesions induced by other carcinogens, but not in regenerative bladder hyperplasias after cyclophosphamide exposure or in bladder exposed to bladder tumor promoters. The latter lesions were also not immunoreactive with antibodies to p21. Our results suggest that this relatively simple technique might be used for identification and screening of tumors for involvement of ras oncogenes and carcinogen initiation. JF - Japanese journal of cancer research : Gann AU - Ward, J M AU - Hagiwara, A AU - Tsuda, H AU - Tatematsu, M AU - Ito, N AD - Tumor Pathology and Pathogenesis Section, National Cancer Institute, Frederick, Maryland 21701-1013. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 152 EP - 155 VL - 79 IS - 2 SN - 0910-5050, 0910-5050 KW - Lectins KW - 0 KW - Peanut Agglutinin KW - Proto-Oncogene Proteins KW - Proto-Oncogene Proteins p21(ras) KW - EC 3.6.5.2 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Hyperplasia KW - Immunohistochemistry KW - Urinary Bladder -- pathology KW - Proto-Oncogene Proteins -- analysis KW - Urinary Bladder Neoplasms -- analysis KW - Proto-Oncogene Proteins -- immunology KW - Urinary Bladder -- analysis KW - Precancerous Conditions -- enzymology KW - Lectins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78203548?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Japanese+journal+of+cancer+research+%3A+Gann&rft.atitle=H-ras+p21+and+peanut+lectin+immunoreactivity+of+hyperplastic%2C+preneoplastic+and+neoplastic+urinary+bladder+lesions+in+rats.&rft.au=Ward%2C+J+M%3BHagiwara%2C+A%3BTsuda%2C+H%3BTatematsu%2C+M%3BIto%2C+N&rft.aulast=Ward&rft.aufirst=J&rft.date=1988-02-01&rft.volume=79&rft.issue=2&rft.spage=152&rft.isbn=&rft.btitle=&rft.title=Japanese+journal+of+cancer+research+%3A+Gann&rft.issn=09105050&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-14 N1 - Date created - 1988-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Voltage-activated sodium conductances in cultured normal and trisomy 16 dorsal root ganglion neurons from the fetal mouse. AN - 78176969; 2452000 AB - Current and voltage clamp recordings were made with a patch-clamp technique from large, light, dorsal root ganglia (DRG) neurons in tissue culture, derived from trisomy 16 and normal fetal mice. In a Na gradient of [52 mM]o/[28 mM]i, the action potential was accelerated, depolarization and repolarization were faster and the total Na conductance was higher in trisomic neurons. A tetrodotoxin (TTX)-sensitive, fast Na current was demonstrated, about 0.9 nA in trisomic and 0.3 nA in control neurons. The calculated mean specific membrane conductances were 0.74 mS/cm2 and 0.28 mS/cm2, respectively. A TTX-insensitive, slow Na conductance, 3-4 times the fast Na conductance and sensitive to Cd, also was demonstrated, with a 2-fold greater current density and conductance in trisomic as compared with control neurons, of 2.22 +/- 0.54 mS/cm2 and 1.26 +/- 0.09 mS/cm2, respectively. The voltage-dependence and kinetics of the TTX-insensitive, slow, Na current were similar in the two neuronal groups. The results indicate that depolarization during the action potential, in fetal mouse DRG neurons in culture, is mediated by this slow TTX-insensitive Na current. Further, acceleration of depolarization in trisomy 16 neurons is caused by a 2-fold increase in the density of the slow Na current. JF - Brain research AU - Orozco, C B AU - Epstein, C J AU - Rapoport, S I AD - National Institute on Aging, NIH, Bethesda, MD 20892. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 265 EP - 274 VL - 466 IS - 2 SN - 0006-8993, 0006-8993 KW - Ion Channels KW - 0 KW - Cobalt KW - 3G0H8C9362 KW - Tetrodotoxin KW - 4368-28-9 KW - Sodium KW - 9NEZ333N27 KW - Index Medicus KW - Animals KW - Cells, Cultured KW - Humans KW - Mice KW - Action Potentials -- drug effects KW - Cobalt -- pharmacology KW - Tetrodotoxin -- pharmacology KW - Chromosomes, Human, Pair 16 KW - Sodium -- physiology KW - Ganglia, Spinal -- physiology KW - Ion Channels -- drug effects KW - Trisomy KW - Ganglia, Spinal -- drug effects KW - Ion Channels -- physiology KW - Sodium -- metabolism KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78176969?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research&rft.atitle=Voltage-activated+sodium+conductances+in+cultured+normal+and+trisomy+16+dorsal+root+ganglion+neurons+from+the+fetal+mouse.&rft.au=Orozco%2C+C+B%3BEpstein%2C+C+J%3BRapoport%2C+S+I&rft.aulast=Orozco&rft.aufirst=C&rft.date=1988-02-01&rft.volume=466&rft.issue=2&rft.spage=265&rft.isbn=&rft.btitle=&rft.title=Brain+research&rft.issn=00068993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-27 N1 - Date created - 1988-05-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Tranylcypromine compared with L-deprenyl in Alzheimer's disease. AN - 78149680; 3127432 AB - The authors have previously reported mild improvement of behavior and cognition in a group of 17 nondepressed patients with dementia of the Alzheimer type (DAT) treated with two doses of L-deprenyl (10 and 40 mg/day). Seven of these patients subsequently received double-blind, placebo-controlled treatment with tranylcypromine. The patients experienced significant side effects, particularly orthostatic hypotension without compensatory pulse increase, during tranylcypromine treatment. These effects were more severe than those occurring during L-deprenyl treatment, and they occurred at substantially lower doses (mean dose, 16 mg/day). These data support the role of the inhibition of monoamine oxidase type A in the development of orthostatic hypotension in patients treated with monoamine oxidase inhibitors. They also raise the question of whether the observed sensitivity to monoamine oxidase inhibition is a function of age or disease. In either case, the greater toxicity of tranylcypromine makes inferences difficult regarding the relative efficacies of these drugs in treating patients with dementia of the Alzheimer type and may limit the potential usefulness of tranylcypromine in ameliorating some symptoms of this disease. JF - Journal of clinical psychopharmacology AU - Tariot, P N AU - Sunderland, T AU - Cohen, R M AU - Newhouse, P A AU - Mueller, E A AU - Murphy, D L AD - Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 23 EP - 27 VL - 8 IS - 1 SN - 0271-0749, 0271-0749 KW - Phenethylamines KW - 0 KW - Selegiline KW - 2K1V7GP655 KW - Tranylcypromine KW - 3E3V44J4Z9 KW - Index Medicus KW - Psychiatric Status Rating Scales KW - Humans KW - Aged KW - Middle Aged KW - Male KW - Female KW - Selegiline -- adverse effects KW - Selegiline -- therapeutic use KW - Alzheimer Disease -- drug therapy KW - Tranylcypromine -- therapeutic use KW - Alzheimer Disease -- psychology KW - Tranylcypromine -- adverse effects KW - Phenethylamines -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78149680?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+internal+medicine&rft.atitle=Factitious+hypoglycemia+due+to+surreptitious+administration+of+insulin.+Diagnosis%2C+treatment%2C+and+long-term+follow-up.&rft.au=Grunberger%2C+G%3BWeiner%2C+J+L%3BSilverman%2C+R%3BTaylor%2C+S%3BGorden%2C+P&rft.aulast=Grunberger&rft.aufirst=G&rft.date=1988-02-01&rft.volume=108&rft.issue=2&rft.spage=252&rft.isbn=&rft.btitle=&rft.title=Annals+of+internal+medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-03 N1 - Date created - 1988-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - CR1-receptor recycling in phorbol ester-activated polymorphonuclear leucocytes. AN - 78146829; 3162434 AB - Complement-receptor type 1, CR1, which recognizes the C3b cleavage fragment of C3, is present on the membranes of human phagocytic cells, but does not mediate phagocytosis or undergo internalization unless activated by one of a variety of stimuli. Among these stimuli low doses of phorbol esters have been shown to induce a consistently increased expression of CR1, despite apparently continuous receptor internalization. We have studied the fate of internalized receptor-ligand complexes in neutrophils activated with low concentrations of phorbol dibutyrate. In our studies, we followed CR1 with either 125I-C3b, the physiologic ligand, or with 125I-Fab fragments of a monoclonal anti-CR1 antibody. We observed rapid internalization of CR1-C3b complexes by PMN treated with 10 ng/ml (1.98 x 10(-8)M) PDBu, consistent in rate and extent with previously reported results using monoclonal antibodies. The fate of the internalized ligand was studied after elution of cell-surface C3b at 0 degrees. Intracellular ligand was externalized in a time- and temperature-dependent fashion, reaching a plateau at 10-15 min. Released C3b was totally TCA precipitable and structurally unaltered, as determined by SDS-PAGE, suggesting that recycling occurs via a prelysosomal predegradative compartment. Loading the cells with chloroquine did not affect this process. A monoclonal anti-CR1 Fab probe behaved in exactly the same manner, suggesting that the recycling of intact ligand-receptor complexes takes place. The possible physiological consequences of this finding are discussed. JF - Immunology AU - Malbran, A AU - Siwik, S AU - Frank, M M AU - Fries, L F AD - Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 325 EP - 330 VL - 63 IS - 2 SN - 0019-2805, 0019-2805 KW - Complement C1 KW - 0 KW - Phorbol Esters KW - Receptors, Complement KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Complement C3b KW - 80295-43-8 KW - Chloroquine KW - 886U3H6UFF KW - Index Medicus KW - Phorbol Esters -- pharmacology KW - Hot Temperature KW - Complement C3b -- immunology KW - Chloroquine -- pharmacology KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - In Vitro Techniques KW - Complement Activation -- drug effects KW - Phagocytosis KW - Time Factors KW - Neutrophils -- immunology KW - Receptors, Complement -- drug effects KW - Complement C1 -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78146829?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Immunology&rft.atitle=CR1-receptor+recycling+in+phorbol+ester-activated+polymorphonuclear+leucocytes.&rft.au=Malbran%2C+A%3BSiwik%2C+S%3BFrank%2C+M+M%3BFries%2C+L+F&rft.aulast=Malbran&rft.aufirst=A&rft.date=1988-02-01&rft.volume=63&rft.issue=2&rft.spage=325&rft.isbn=&rft.btitle=&rft.title=Immunology&rft.issn=00192805&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-29 N1 - Date created - 1988-04-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Arch Biochem Biophys. 1969 Nov;134(2):279-84 [4982185] J Immunol. 1985 Nov;135(5):3381-7 [2931483] J Immunol. 1979 Oct;123(4):1839-46 [573303] Biochem Biophys Res Commun. 1980 Mar 13;93(1):1-8 [7378072] J Immunol. 1980 Aug;125(2):844-9 [7391581] Methods Enzymol. 1980;70(A):142-50 [6775174] J Biol Chem. 1981 Apr 25;256(8):3995-4006 [6783652] J Exp Med. 1981 Jun 1;153(6):1615-28 [7252422] J Cell Biol. 1982 Feb;92(2):417-24 [6277962] J Immunol. 1983 Jan;130(1):370-5 [6847888] J Exp Med. 1982 Oct 1;156(4):1149-64 [7153708] J Exp Med. 1983 Jun 1;157(6):1844-54 [6854210] J Exp Med. 1983 Oct 1;158(4):1338-43 [6225825] J Clin Invest. 1983 Nov;72(5):1793-800 [6415117] J Biol Chem. 1984 Feb 10;259(3):1703-13 [6693431] J Exp Med. 1984 Jan 1;159(1):137-51 [6319529] J Cell Biol. 1984 Apr;98(4):1163-9 [6715403] Biochim Biophys Acta. 1984 Nov 13;805(3):268-76 [6148970] J Clin Invest. 1984 Nov;74(5):1566-71 [6209300] J Immunol. 1985 Mar;134(3):1851-8 [3155775] J Exp Med. 1985 May 1;161(5):912-23 [3157764] J Immunol. 1985 Aug;135(2):1325-30 [3159791] J Immunol. 1985 Oct;135(4):2673-9 [3161945] Proc Natl Acad Sci U S A. 1970 Jul;66(3):651-6 [4913206] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neuropsychological performance and treatment outcome in male alcoholics. AN - 78139855; 3279865 AB - Commonly used neuropsychological tests were administered to 91 detoxified alcoholics at the beginning of treatment. Statistically significant relationships were observed between test scores and post-treatment consumption determined 8 months after completing treatment for 72 patients. The results varied depending upon the particular measure of posttreatment consumption evaluated and the type of statistical analysis used. The most consistent relationships were often counter to the notion that increased neuropsychological performance is correlated with a more favorable treatment outcome. Neuropsychological evaluation is of limited clinical utility in predicting posttreatment alcohol consumption. JF - Alcoholism, clinical and experimental research AU - Eckardt, M J AU - Rawlings, R R AU - Graubard, B I AU - Faden, V AU - Martin, P R AU - Gottschalk, L A AD - Division of Biometry and Epidemiology, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 88 EP - 93 VL - 12 IS - 1 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Humans KW - Adult KW - Middle Aged KW - Follow-Up Studies KW - Psychometrics KW - Male KW - Alcoholism -- rehabilitation KW - Substance-Related Disorders -- rehabilitation KW - Substance-Related Disorders -- psychology KW - Neuropsychological Tests KW - Alcoholism -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78139855?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Neuropsychological+performance+and+treatment+outcome+in+male+alcoholics.&rft.au=Eckardt%2C+M+J%3BRawlings%2C+R+R%3BGraubard%2C+B+I%3BFaden%2C+V%3BMartin%2C+P+R%3BGottschalk%2C+L+A&rft.aulast=Eckardt&rft.aufirst=M&rft.date=1988-02-01&rft.volume=12&rft.issue=1&rft.spage=88&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-08 N1 - Date created - 1988-04-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Tolerance and the etiology of alcoholism: hypothesis and mechanism. AN - 78128533; 2964796 JF - Alcoholism, clinical and experimental research AU - Tabakoff, B AU - Hoffman, P L AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, NIH, Bethesda, MD 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 184 EP - 186 VL - 12 IS - 1 SN - 0145-6008, 0145-6008 KW - Neuropeptides KW - 0 KW - Receptors, Angiotensin KW - Receptors, Vasopressin KW - Arginine Vasopressin KW - 113-79-1 KW - Index Medicus KW - Neuropeptides -- physiology KW - Drug Tolerance KW - Animals KW - Receptors, Angiotensin -- physiology KW - Humans KW - Arginine Vasopressin -- physiology KW - Brain -- physiopathology KW - Alcoholism -- physiopathology KW - Alcohol Drinking -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78128533?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Tolerance+and+the+etiology+of+alcoholism%3A+hypothesis+and+mechanism.&rft.au=Tabakoff%2C+B%3BHoffman%2C+P+L&rft.aulast=Tabakoff&rft.aufirst=B&rft.date=1988-02-01&rft.volume=12&rft.issue=1&rft.spage=184&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-08 N1 - Date created - 1988-04-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Histopathological effects of intraoperative radiotherapy on pancreas and adjacent tissues: a postmortem analysis. AN - 78127838; 3343837 AB - Intraoperative radiotherapy (IORT) has been utilized in the treatment of resectable and unresectable pancreatic carcinoma at the National Cancer Institute. Detailed autopsy analyses of the radiation effects on the pancreas and adjacent tissues were performed on 13 patients dying at various times following therapy. IORT can induce a progressive retroperitoneal fibrosis and fibrosis of the porta hepatis in patients with resectable pancreatic carcinoma. In unresectable pancreatic carcinoma, the major expression of intraoperative irradiation with external beam irradiation is a progressive fibrosis of the pancreas with vascular sclerosis, nerve degeneration, atrophy of acinar cells, and atypical changes in the ducts of the pancreas, as well as degenerative changes of the pancreatic tumor. JF - Journal of surgical oncology AU - Hoekstra, H J AU - Restrepo, C AU - Kinsella, T J AU - Sindelar, W F AD - Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 104 EP - 108 VL - 37 IS - 2 SN - 0022-4790, 0022-4790 KW - Index Medicus KW - Adrenal Glands -- radiation effects KW - Liver -- pathology KW - Humans KW - Bile Ducts -- radiation effects KW - Aged KW - Blood Vessels -- pathology KW - Adrenal Glands -- pathology KW - Spinal Cord -- radiation effects KW - Retroperitoneal Space -- radiation effects KW - Radiotherapy Dosage KW - Intraoperative Care KW - Adult KW - Bile Ducts -- pathology KW - Blood Vessels -- radiation effects KW - Spinal Cord -- pathology KW - Retroperitoneal Space -- pathology KW - Middle Aged KW - Male KW - Liver -- radiation effects KW - Female KW - Pancreas -- pathology KW - Pancreatic Neoplasms -- radiotherapy KW - Pancreatic Neoplasms -- pathology KW - Pancreas -- radiation effects KW - Radiotherapy -- adverse effects KW - Pancreatic Neoplasms -- surgery UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78127838?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+surgical+oncology&rft.atitle=Histopathological+effects+of+intraoperative+radiotherapy+on+pancreas+and+adjacent+tissues%3A+a+postmortem+analysis.&rft.au=Hoekstra%2C+H+J%3BRestrepo%2C+C%3BKinsella%2C+T+J%3BSindelar%2C+W+F&rft.aulast=Hoekstra&rft.aufirst=H&rft.date=1988-02-01&rft.volume=37&rft.issue=2&rft.spage=104&rft.isbn=&rft.btitle=&rft.title=Journal+of+surgical+oncology&rft.issn=00224790&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-04 N1 - Date created - 1988-04-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Drug purpura due to surreptitious quinidine intake. AN - 78119299; 3341652 AB - Three patients had recurrent episodes of thrombocytopenia that resembled drug purpura, but the drug history in each case did not support the diagnosis. Although the patients specifically denied taking quinidine, serologic testing with this drug was done because the patients had access to it, and it is the commonest cause of drug purpura. Highly specific quinidine-dependent antiplatelet antibodies were found in the sera of all three patients. After being informed of the laboratory findings, the patients have had no recurrences of purpura. Serologic tests for quinidine- or quinine-dependent antibodies can help elucidate some obscure cases of purpura that may be self-induced. JF - Annals of internal medicine AU - Reid, D M AU - Shulman, N R AD - National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 206 EP - 208 VL - 108 IS - 2 SN - 0003-4819, 0003-4819 KW - Antibodies KW - 0 KW - Quinidine KW - ITX08688JL KW - Abridged Index Medicus KW - Index Medicus KW - Antibody Specificity KW - Complement Fixation Tests KW - Blood Platelets -- immunology KW - Humans KW - Aged KW - Middle Aged KW - Antibodies -- analysis KW - Male KW - Female KW - Quinidine -- immunology KW - Purpura, Thrombocytopenic -- chemically induced KW - Quinidine -- poisoning KW - Factitious Disorders -- diagnosis KW - Quinidine -- blood KW - Purpura, Thrombocytopenic -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78119299?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+internal+medicine&rft.atitle=Drug+purpura+due+to+surreptitious+quinidine+intake.&rft.au=Reid%2C+D+M%3BShulman%2C+N+R&rft.aulast=Reid&rft.aufirst=D&rft.date=1988-02-01&rft.volume=108&rft.issue=2&rft.spage=206&rft.isbn=&rft.btitle=&rft.title=Annals+of+internal+medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-11 N1 - Date created - 1988-03-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Factitious hypoglycemia due to surreptitious administration of insulin. Diagnosis, treatment, and long-term follow-up. AN - 78109607; 3277509 AB - Ten patients had factitious hypoglycemia due to surreptitious insulin injections diagnosed and were followed for up to 15 years (median, 5 years; range, 2 months to 15 years). When available, demonstration of anti-insulin antibodies was the most helpful diagnostic test. Decreased plasma C-peptide levels corroborated the diagnosis. Young women (nine of ten) with knowledge of the medical profession or relatives with diabetes mellitus predominated in the sample. Five of the patients had a history of insulin-requiring diabetes mellitus. Two patients eventually committed suicide despite the best efforts at therapy. Only three of ten patients made a successful transition into productive life after the diagnosis of factitious hypoglycemia was established. Factitious hypoglycemia remains a difficult diagnosis to make, and the long-term outcome after the diagnosis is established is unpredictable. All efforts have to be made to confirm the diagnosis before the patients are approached. The confrontation is to be made by an experienced team of health care professionals who have gained the patient's confidence through an understanding but firm manner. Long-term therapy must be planned and initiated before the patient's discharge. JF - Annals of internal medicine AU - Grunberger, G AU - Weiner, J L AU - Silverman, R AU - Taylor, S AU - Gorden, P AD - Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 252 EP - 257 VL - 108 IS - 2 SN - 0003-4819, 0003-4819 KW - C-Peptide KW - 0 KW - Insulin KW - Insulin Antibodies KW - Abridged Index Medicus KW - Index Medicus KW - Insulin Antibodies -- analysis KW - C-Peptide -- blood KW - Humans KW - Adult KW - Follow-Up Studies KW - Male KW - Female KW - Factitious Disorders -- therapy KW - Insulin -- blood KW - Factitious Disorders -- diagnosis KW - Insulin -- administration & dosage KW - Hypoglycemia -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78109607?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+internal+medicine&rft.atitle=Factitious+hypoglycemia+due+to+surreptitious+administration+of+insulin.+Diagnosis%2C+treatment%2C+and+long-term+follow-up.&rft.au=Grunberger%2C+G%3BWeiner%2C+J+L%3BSilverman%2C+R%3BTaylor%2C+S%3BGorden%2C+P&rft.aulast=Grunberger&rft.aufirst=G&rft.date=1988-02-01&rft.volume=108&rft.issue=2&rft.spage=252&rft.isbn=&rft.btitle=&rft.title=Annals+of+internal+medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-11 N1 - Date created - 1988-03-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - IgE immunotoxins. Effect of an IgE-ricin A chain conjugate on rat skin histamine content. AN - 78096391; 2448377 AB - Immunotoxins--toxins covalently conjugated to specific antibodies--have been studied as possible agents in the treatment of cancer. The avid binding of IgE antibodies to FcR on mast cells and basophils suggested the possible use of an IgE-immunotoxin in the treatment of malignant mastocytosis or as a method to generate mast cell-depleted animals for study. To this end, the effect of a covalent conjugate of rat myeloma IgE and ricin A chain on rat cutaneous mast cells was examined in vivo. IgE-ricin A chain was capable of binding to and sensitizing cutaneous mast cells in vivo as indicated by a bluing response to intracutaneous anti-ricin A chain. IgE-ricin A chain, given either as a single dose or, even more effectively, as two split doses, significantly reduced cutaneous histamine content for 6 to 8 days. Neither a mixture of IgE and ricin A chain that were not conjugated nor the induction of cutaneous mast cell degranulation with anti-IgE affected cutaneous histamine levels. Therefore, IgE-ricin A chain produces a prolonged depletion of cutaneous histamine levels. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Slater, J E AU - Boltansky, H AU - Kaliner, M AD - Allergic Diseases Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 807 EP - 811 VL - 140 IS - 3 SN - 0022-1767, 0022-1767 KW - Immunotoxins KW - 0 KW - Immunoglobulin E KW - 37341-29-0 KW - Ricin KW - 9009-86-3 KW - Abridged Index Medicus KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Protein Biosynthesis KW - Animals KW - Mast Cells -- immunology KW - Leukemia, Experimental -- immunology KW - Electrophoresis, Polyacrylamide Gel KW - Dose-Response Relationship, Immunologic KW - Basophils -- immunology KW - Immunoglobulin E -- physiology KW - Skin -- immunology KW - Immunotoxins -- chemical synthesis KW - Immunotoxins -- isolation & purification KW - Immunotoxins -- pharmacology KW - Ricin -- pharmacology KW - Histamine Release -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78096391?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=IgE+immunotoxins.+Effect+of+an+IgE-ricin+A+chain+conjugate+on+rat+skin+histamine+content.&rft.au=Slater%2C+J+E%3BBoltansky%2C+H%3BKaliner%2C+M&rft.aulast=Slater&rft.aufirst=J&rft.date=1988-02-01&rft.volume=140&rft.issue=3&rft.spage=807&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-15 N1 - Date created - 1988-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cytogenetic studies in patients with secondary leukemia/dysmyelopoietic syndrome after different treatment modalities. AN - 78096347; 3337904 AB - Cytogenetic studies of 68 patients who developed secondary leukemia (SL)/dysmyelopoietic syndrome (DMS) after extensive chemotherapy and/or radiation therapy as well as patients who developed SL/DMS without such treatment showed that those patients who received radiation alone or with chemotherapy had more extensive numerical and structural abnormalities than those who received only chemotherapy. In terms of the specific chromosomal abnormalities, there are no differences between the various treatment groups. Hypodiploidy is the most common form of aneuploidy in these patients, with the most common numerical abnormality being the loss of chromosome 7. The most common structural abnormalities involved chromosomes 3 and 5. When compared with patients with de novo leukemia and DMS, the chromosomal abnormalities in these patients are more complex and extensive. Serial studies revealed that cytogenetic abnormalities do not precede the development of hematologic changes by significant time periods. JF - Blood AU - Whang-Peng, J AU - Young, R C AU - Lee, E C AU - Longo, D L AU - Schechter, G P AU - DeVita, V T AD - Medicine Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 403 EP - 414 VL - 71 IS - 2 SN - 0006-4971, 0006-4971 KW - Antineoplastic Agents KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - Lymphoma, Non-Hodgkin -- therapy KW - Chromosome Banding KW - Humans KW - Chromosome Disorders KW - Time Factors KW - Hodgkin Disease -- therapy KW - Chromosomes, Human, Pair 7 KW - Leukemia -- chemically induced KW - Chromosome Aberrations -- etiology KW - Myeloproliferative Disorders -- genetics KW - Leukemia, Radiation-Induced -- genetics KW - Myeloproliferative Disorders -- chemically induced KW - Leukemia -- genetics KW - Antineoplastic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78096347?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Cytogenetic+studies+in+patients+with+secondary+leukemia%2Fdysmyelopoietic+syndrome+after+different+treatment+modalities.&rft.au=Whang-Peng%2C+J%3BYoung%2C+R+C%3BLee%2C+E+C%3BLongo%2C+D+L%3BSchechter%2C+G+P%3BDeVita%2C+V+T&rft.aulast=Whang-Peng&rft.aufirst=J&rft.date=1988-02-01&rft.volume=71&rft.issue=2&rft.spage=403&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-04 N1 - Date created - 1988-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Combination chemotherapy with mastectomy or radiotherapy for stage III breast carcinoma: a Cancer and Leukemia Group B study. AN - 78095693; 3276824 AB - One hundred thirteen evaluable patients with previously untreated stage III breast carcinoma were treated with three monthly cycles of cyclophosphamide (CYC), doxorubicin (DOX), 5-fluorouracil (5-FU), vincristine (VCR), and prednisone (PRED) (CAFVP). Subsequently, 91 (81%) were deemed operable. Patients were then randomized to receive surgery or radiotherapy (RT) to determine which of these modalities afforded better local tumor control. All patients also received 2 additional years of CAFVP in a further attempt to eradicate local disease and systemic micrometastases. Forty-one of the randomized patients have relapsed. Approximately half of the initial relapses in each arm were local. The overall duration of disease control was similar following either modality, with a median of 29.2 months for surgery patients and 24.4 months for RT patients. Similarly, there was no major difference in survival related to randomized treatment with an overall median of 39 months (median follow-up 37 months). Pre- or perimenopausal status and inflammatory disease were associated with shorter disease control and survival. Treatment was generally well tolerated and toxicity was acceptable. This study demonstrates that prolonged control of stage III breast carcinoma can be achieved with combined modality therapy in which cytotoxic chemotherapy precedes and follows treatment directly primarily at the breast tumor, using either surgery or RT. Nevertheless, new regimens must be designed if significant advances that may lead to the cure of this disease are to be achieved. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Perloff, M AU - Lesnick, G J AU - Korzun, A AU - Chu, F AU - Holland, J F AU - Thirlwell, M P AU - Ellison, R R AU - Carey, R W AU - Leone, L AU - Weinberg, V AD - Cancer and Leukemia Group B, National Institutes of Health, Bethesda, MD 20892-4200. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 261 EP - 269 VL - 6 IS - 2 SN - 0732-183X, 0732-183X KW - Index Medicus KW - Neoplasm Staging KW - Random Allocation KW - Combined Modality Therapy KW - Humans KW - Adult KW - Clinical Trials as Topic KW - Aged KW - Middle Aged KW - Neoplasm Recurrence, Local KW - Female KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- pathology KW - Mastectomy KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Breast Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78095693?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Combination+chemotherapy+with+mastectomy+or+radiotherapy+for+stage+III+breast+carcinoma%3A+a+Cancer+and+Leukemia+Group+B+study.&rft.au=Perloff%2C+M%3BLesnick%2C+G+J%3BKorzun%2C+A%3BChu%2C+F%3BHolland%2C+J+F%3BThirlwell%2C+M+P%3BEllison%2C+R+R%3BCarey%2C+R+W%3BLeone%2C+L%3BWeinberg%2C+V&rft.aulast=Perloff&rft.aufirst=M&rft.date=1988-02-01&rft.volume=6&rft.issue=2&rft.spage=261&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-24 N1 - Date created - 1988-03-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Molecular complexes of thyroid hormone tyrosyl rings with aromatic donors. Possible relationship to receptor protein interactions. AN - 78095109; 2828621 AB - Several lines of evidence have indicated that thyroid hormones share common molecular properties (accessible planar face and lateral halogenation) with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds of environmental importance and can modulate their toxicity. Binding of dioxin to a soluble intracellular protein (dioxin or Ah receptor) appears to be the initial step in their mechanism of toxicity and a stacking interaction model has been proposed at the molecular level. It has also been recognized that the Ah receptor and the triiodothyronine nuclear receptor share certain physical and chemical properties important in their binding interactions. In this work, we examined the possibility that thyroid hormones might also be able to bind by a stacking complexation mechanism. By use of methods based on nuclear magnetic resonance spectroscopy, selected, structurally distinct thyroid hormone analogues with widely different hormonal activities were shown to function as electron acceptors in molecular complexes with aromatic donors involving the nonphenolic or tyrosyl ring. Binding free energies for these complexes correlated well with those previously reported for the triiodothyronine (L-T3) nuclear receptor binding interaction with the same compounds. This included preference for L-T3 over thyroxine (L-T4), very favorable binding of 3,5,3'-triiodothyroacetic acid (Triac), and marked preference for L-T3 over D-T3. These results suggest that a considerable part of the structural specificity in thyroid hormone action may be mediated by the tyrosyl ring interaction. Binding ligands for the triiodothyronine nuclear receptor and the Ah receptor may share common molecular parameters in the expression of their binding activities. JF - Journal of medicinal chemistry AU - Chae, K AU - McKinney, J D AD - Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 357 EP - 362 VL - 31 IS - 2 SN - 0022-2623, 0022-2623 KW - Receptors, Aryl Hydrocarbon KW - 0 KW - Receptors, Drug KW - Receptors, Thyroid Hormone KW - Thyroid Hormones KW - Tyrosine KW - 42HK56048U KW - Index Medicus KW - Receptors, Drug -- metabolism KW - Molecular Conformation KW - Structure-Activity Relationship KW - Magnetic Resonance Spectroscopy KW - Thyroid Hormones -- pharmacology KW - Receptors, Thyroid Hormone -- metabolism KW - Tyrosine -- metabolism KW - Thyroid Hormones -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78095109?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medicinal+chemistry&rft.atitle=Molecular+complexes+of+thyroid+hormone+tyrosyl+rings+with+aromatic+donors.+Possible+relationship+to+receptor+protein+interactions.&rft.au=Chae%2C+K%3BMcKinney%2C+J+D&rft.aulast=Chae&rft.aufirst=K&rft.date=1988-02-01&rft.volume=31&rft.issue=2&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Journal+of+medicinal+chemistry&rft.issn=00222623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-16 N1 - Date created - 1988-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alpha 1-adrenergic potentiation of vasoactive intestinal peptide stimulation of rat pinealocyte adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate: evidence for a role of calcium and protein kinase-C. AN - 78094835; 2892667 AB - alpha 1-Adrenergic agonists have recently been found to potentiate vasoactive intestinal peptide (VIP) stimulation of rat pinealocyte cAMP and cGMP. alpha 1-Adrenergic agonists also elevate pineal intracellular Ca2+ [( Ca2+]i) and activate protein kinase-C. In the present study, the possible involvement of Ca2+ and protein kinase-C in the alpha 1-adrenergic potentiation of VIP-stimulated cAMP and cGMP accumulation was examined with agents that alter [Ca2+]i or activate protein kinase-C. It was found that treatment with a Ca2+ chelator or with inorganic Ca2+ channel blockers inhibited alpha 1-adrenergic potentiation of VIP-stimulated cAMP and cGMP responses. Increasing [Ca2+]i by treatment with A23187, ouabain, or K+ potentiated VIP stimulation of cAMP and cGMP response. These observations indicate that Ca2+ mediates the alpha 1-adrenergic potentiation of VIP-stimulated cAMP and cGMP accumulation, as is true for the alpha 1-adrenergic potentiation of beta-adrenergic stimulated cAMP and cGMP accumulation. Activators of protein kinase-C mimicked the large effect alpha 1-adrenergic agonists have on cAMP accumulation in VIP-treated pinealocytes and had a small effect on cGMP accumulation in VIP-treated cells. These effects were not blocked by the Ca2+ chelator EGTA. However, the effects of a protein kinase-C activator on the cGMP response in VIP-stimulated cells were amplified by K+ (15 mM) or ouabain (1 microM), presumably through an action causing an increase in [Ca2+]i. These results suggest protein kinase-C is involved in the alpha 1-adrenergic potentiation of VIP-stimulated cAMP accumulation, as is the case for the alpha 1-adrenergic potentiation of beta-adrenergic stimulated cAMP. Protein kinase-C is also involved in cGMP accumulation, provided that there is a modest increase in [Ca2+]i. JF - Endocrinology AU - Chik, C L AU - Ho, A K AU - Klein, D C AD - Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 702 EP - 708 VL - 122 IS - 2 SN - 0013-7227, 0013-7227 KW - Adrenergic alpha-Agonists KW - 0 KW - Vasoactive Intestinal Peptide KW - 37221-79-7 KW - Calcimycin KW - 37H9VM9WZL KW - Ouabain KW - 5ACL011P69 KW - Cyclic AMP KW - E0399OZS9N KW - Protein Kinase C KW - EC 2.7.11.13 KW - Cyclic GMP KW - H2D2X058MU KW - Nifedipine KW - I9ZF7L6G2L KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Potassium KW - RWP5GA015D KW - Calcium KW - SY7Q814VUP KW - Norepinephrine KW - X4W3ENH1CV KW - Abridged Index Medicus KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Nifedipine -- pharmacology KW - Animals KW - Norepinephrine -- pharmacology KW - Dose-Response Relationship, Drug KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Potassium -- pharmacology KW - Calcimycin -- pharmacology KW - Drug Synergism KW - Ouabain -- pharmacology KW - Female KW - Vasoactive Intestinal Peptide -- pharmacology KW - Protein Kinase C -- metabolism KW - Calcium -- metabolism KW - Pineal Gland -- metabolism KW - Cyclic GMP -- metabolism KW - Adrenergic alpha-Agonists -- pharmacology KW - Cyclic AMP -- metabolism KW - Pineal Gland -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78094835?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Detection+of+activated+proto-oncogenes+in+N-nitrosodiethylamine-induced+liver+tumors%3A+a+comparison+between+B6C3F1+mice+and+Fischer+344+rats.&rft.au=Stowers%2C+S+J%3BWiseman%2C+R+W%3BWard%2C+J+M%3BMiller%2C+E+C%3BMiller%2C+J+A%3BAnderson%2C+M+W%3BEva%2C+A&rft.aulast=Stowers&rft.aufirst=S&rft.date=1988-02-01&rft.volume=9&rft.issue=2&rft.spage=271&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-03 N1 - Date created - 1988-03-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Selective effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and corticosteroid on in vitro lymphocyte maturation. AN - 78092617; 3257509 AB - The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the corticosteroid dexamethasone have potent effects on lymphocyte function, although the effects of the former have not been well characterized. In the present studies murine B cell maturation was used as a model system to examine and compare the effects of TCDD and dexamethasone on cell function. Immunosuppression by TCDD and dexamethasone is mediated by binding to specific intracellular R referred to as the Ah and glucocorticoid R, respectively. Although both compounds were comparable in their ability to inhibit antibody responses to the T-independent antigen TNP-LPS, the events responsible for suppression were found to be distinct. Dexamethasone, although affecting multiple stages of B cell maturation, had its primary effect very early, manifested by inhibition of the phosphoinositide signal transduction pathway. This was evidenced by a decrease in accumulation of inositol phosphate and surface Ia antigen expression as well as an inability to enter the cell cycle after stimulation with anti-Ig. In contrast, neither early signaling events nor proliferation were affected in B cells treated with TCDD. However, TCDD inhibited Ig secretion after stimulation of B cells with T cell-replacing factor, suggesting that TCDD modulates the differentiation of B cells into plasma cells. These differential results were confirmed by monitoring the expression of surface antigens that occur on B cells, including Ia, 7D4, and PC.2, during this maturational process. Whereas dexamethasone inhibited the expression of surface antigens that occur early in maturation (Ia and 7D4), TCDD blocked only the expression of the plasma cell marker PC.2. Although TCDD altered later stages of the B cell cycle, the presence of TCDD was required at the time of initial activation to be effective, suggesting that TCDD may interfere with early cell programming. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Luster, M I AU - Germolec, D R AU - Clark, G AU - Wiegand, G AU - Rosenthal, G J AD - Immunotoxicology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 928 EP - 935 VL - 140 IS - 3 SN - 0022-1767, 0022-1767 KW - Antigens, Differentiation, B-Lymphocyte KW - 0 KW - Antigens, T-Independent KW - Dioxins KW - Immunoglobulin M KW - Immunosuppressive Agents KW - Lipopolysaccharides KW - Phospholipids KW - Polychlorinated Dibenzodioxins KW - trinitrophenyl-lipopolysaccharide KW - Dexamethasone KW - 7S5I7G3JQL KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Phospholipids -- metabolism KW - Mice KW - Antigens, Differentiation, B-Lymphocyte -- analysis KW - Mice, Inbred DBA KW - Immunosuppressive Agents -- pharmacology KW - Cell Survival -- drug effects KW - Lipopolysaccharides -- immunology KW - Mice, Inbred C57BL KW - Immunoglobulin M -- biosynthesis KW - Antigens, T-Independent -- immunology KW - Antibody Formation -- drug effects KW - Cell Cycle -- drug effects KW - Female KW - Lymphocyte Activation -- drug effects KW - B-Lymphocytes -- drug effects KW - Dexamethasone -- pharmacology KW - Polychlorinated Dibenzodioxins -- pharmacology KW - B-Lymphocytes -- immunology KW - B-Lymphocytes -- metabolism KW - Dioxins -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78092617?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Selective+effects+of+2%2C3%2C7%2C8-tetrachlorodibenzo-p-dioxin+and+corticosteroid+on+in+vitro+lymphocyte+maturation.&rft.au=Luster%2C+M+I%3BGermolec%2C+D+R%3BClark%2C+G%3BWiegand%2C+G%3BRosenthal%2C+G+J&rft.aulast=Luster&rft.aufirst=M&rft.date=1988-02-01&rft.volume=140&rft.issue=3&rft.spage=928&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-15 N1 - Date created - 1988-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Bupropion in depression. II. The role of metabolites in clinical outcome. AN - 78089362; 3122699 AB - We studied the steady-state pharmacokinetics of bupropion hydrochloride, a unicyclic aminoketone antidepressant, in depressed patients. The metabolites hydroxybupropion (HB), threohydrobupropion, and erythrohydrobupropion predominated over the parent compound in plasma and cerebrospinal fluid at steady state. Plasma concentrations of each metabolite correlated with cerebrospinal fluid concentrations. Higher plasma metabolite concentrations were associated with poor clinical outcome. This relationship was most striking with HB; plasma HB levels were greater than 1250 ng/mL in all five nonresponders and less than 1200 ng/mL in all seven responders. Plasma HB levels correlated with postreatment plasma homovanillic acid levels. High levels of bupropion metabolites may be associated with poor clinical outcome due to toxic effects involving dopaminergic systems. Alternatively, a curvilinear dose-response relationship may exist for bupropion metabolites. Future studies should explore the clinical utility of plasma metabolite measurements in enhancing the efficacy of treatment with bupropion. JF - Archives of general psychiatry AU - Golden, R N AU - De Vane, C L AU - Laizure, S C AU - Rudorfer, M V AU - Sherer, M A AU - Potter, W Z AD - Section on Clinical Pharmacology, National Institute of Mental Health, Bethesda, MD. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 145 EP - 149 VL - 45 IS - 2 SN - 0003-990X, 0003-990X KW - Propiophenones KW - 0 KW - hydroxybupropion KW - Bupropion KW - 01ZG3TPX31 KW - Abridged Index Medicus KW - Index Medicus KW - Half-Life KW - Double-Blind Method KW - Humans KW - Adult KW - Outcome and Process Assessment (Health Care) KW - Clinical Trials as Topic KW - Aged KW - Middle Aged KW - Male KW - Female KW - Propiophenones -- metabolism KW - Bupropion -- therapeutic use KW - Propiophenones -- therapeutic use KW - Propiophenones -- pharmacokinetics KW - Depressive Disorder -- drug therapy KW - Bupropion -- metabolism KW - Bupropion -- analogs & derivatives KW - Depressive Disorder -- metabolism KW - Bupropion -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78089362?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+general+psychiatry&rft.atitle=Bupropion+in+depression.+II.+The+role+of+metabolites+in+clinical+outcome.&rft.au=Golden%2C+R+N%3BDe+Vane%2C+C+L%3BLaizure%2C+S+C%3BRudorfer%2C+M+V%3BSherer%2C+M+A%3BPotter%2C+W+Z&rft.aulast=Malbran&rft.aufirst=A&rft.date=1988-02-01&rft.volume=63&rft.issue=2&rft.spage=325&rft.isbn=&rft.btitle=&rft.title=Immunology&rft.issn=00192805&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-24 N1 - Date created - 1988-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Early superoxide dismutase-sensitive event promotes neoplastic transformation in mouse epidermal JB6 cells. AN - 78086600; 2827903 AB - Evidence has been obtained that implicates the generation of reactive oxygen species as an early and critical event in the promotion of neoplastic transformation in mouse JB6 cells. The time courses for specific inhibition by CuZn-superoxide dismutase (CuZn-SOD) of the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced promotion of neoplastic transformation in JB6 cells and for changes in antioxidant enzyme activities associated with TPA-exposure were examined. The antipromoting effect of CuZn-SOD was found to be critically dependent on the time of addition of CuZn-SOD relative to the start of a 14-day exposure of cells to TPA. Treatment of JB6 P+ Clone 22 and Clone 41 cells with CuZn-SOD for 18 h before, simultaneously with or up to 1 h after exposure to TPA, all inhibited promotion of transformation maximally. Delay of addition of CuZn-SOD by 2 h or more after the start of TPA treatment resulted in a marked decrease in the promotion inhibitory effect. CuZn-SOD added 24 or 48 h after TPA had no effect on promotion of transformation. Exposure of JB6 cells to 0.2- (superoxide anion radical) generated exogenously by the aerobic xanthine oxidase reaction resulted in promotion of neoplastic transformation that was prevented by concurrent addition of CuZn-SOD. Taken together these studies provide evidence that increased superoxide anion generation within the first 2 h following TPA exposure is an essential event in promotion of transformation in JB6 cells. Upon TPA exposure, JB6 Clone 41 cells exhibited time-specific activity changes in the cellular SOD, glutathione peroxidase (GSH-Px), and catalase. SOD and GSH-Px activities were reduced to 54% and 26% respectively of basal levels within 2 h of TPA treatment. GSH-Px activity recovered to basal levels within 4 h and CuZn-SOD within 48 h. Catalase activity was maximally reduced to 50% of basal within 1 h after TPA treatment and rebounded to greater than basal levels within 4 h. It is postulated that a c-kinase-dependent event induces rapid elevation of superoxide anion following TPA exposure and that this leads to reduced activity of antioxidant enzymes. Since antipromotion by exogenous CuZn-SOD is effective only during the first 2 h following TPA exposure, this suggests that the promotion-relevant 0.2- elevation is transient. JF - Carcinogenesis AU - Nakamura, Y AU - Gindhart, T D AU - Winterstein, D AU - Tomita, I AU - Seed, J L AU - Colburn, N H AD - Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research Facility, MD 21701-1013. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 203 EP - 207 VL - 9 IS - 2 SN - 0143-3334, 0143-3334 KW - Carcinogens KW - 0 KW - Superoxides KW - 11062-77-4 KW - Glutathione Peroxidase KW - EC 1.11.1.9 KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Animals KW - Mice KW - Glutathione Peroxidase -- antagonists & inhibitors KW - Cell Line KW - Superoxide Dismutase -- antagonists & inhibitors KW - Cell Transformation, Neoplastic -- drug effects KW - Cell Transformation, Neoplastic -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78086600?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Japanese+journal+of+cancer+research+%3A+Gann&rft.atitle=H-ras+p21+and+peanut+lectin+immunoreactivity+of+hyperplastic%2C+preneoplastic+and+neoplastic+urinary+bladder+lesions+in+rats.&rft.au=Ward%2C+J+M%3BHagiwara%2C+A%3BTsuda%2C+H%3BTatematsu%2C+M%3BIto%2C+N&rft.aulast=Ward&rft.aufirst=J&rft.date=1988-02-01&rft.volume=79&rft.issue=2&rft.spage=152&rft.isbn=&rft.btitle=&rft.title=Japanese+journal+of+cancer+research+%3A+Gann&rft.issn=09105050&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-02 N1 - Date created - 1988-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Detection of activated proto-oncogenes in N-nitrosodiethylamine-induced liver tumors: a comparison between B6C3F1 mice and Fischer 344 rats. AN - 78081994; 2827904 AB - DNA from B6C3F1 mouse and Fischer 344 rat liver tumors induced by N-nitrosodiethylamine (DEN) were examined for the ability to induce morphological transformation of NIH3T3 cells. DNAs from 14 of 33 of the mouse liver tumors induced by a single injection of DEN at 12 or 15 days of age were positive in this assay while DNA from only one of 28 DEN-induced rat liver tumors was active. Southern blot analysis of the NIH3T3 transformants derived from the mouse liver tumors revealed amplified and/or rearranged restriction fragments homologous to the H-ras proto-oncogene. DNA from two independent foci induced by the rat tumor DNA did not hybridize to probes for members of the ras gene family or c-raf. Activating mutations in the H-ras genes from the DEN-induced mouse liver tumors were characterized by selective oligonucleotide hybridization and the detection of a new XbaI restriction site by Southern blot analysis. In activated H-ras genes from the DEN-induced mouse liver tumor DNA, seven of 14 had a CG----AT transversion at the first base of the 61st codon, three of 14 had an AT----GC transition and four of 14 had the AT----TA transversion at the second base of codon 61. This spectrum of mutations is very similar to that recently observed in activated H-ras genes found in spontaneously occurring B6C3F1 mouse liver tumors. Taken together, the data suggest that the DEN-induced rat and mouse liver carcinogenesis may involve genetic targets other than or in addition to the H-ras gene. JF - Carcinogenesis AU - Stowers, S J AU - Wiseman, R W AU - Ward, J M AU - Miller, E C AU - Miller, J A AU - Anderson, M W AU - Eva, A AD - National Institute of Environmental Health Sciences, Laboratory of Biochemical Risk Analysis, Research Triangle Park, NC 27709. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 271 EP - 276 VL - 9 IS - 2 SN - 0143-3334, 0143-3334 KW - Codon KW - 0 KW - DNA, Neoplasm KW - Diethylnitrosamine KW - 3IQ78TTX1A KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Index Medicus KW - Rats KW - Mice, Inbred Strains KW - Animals KW - Rats, Inbred F344 KW - Mice KW - Nucleic Acid Hybridization KW - Mutation KW - Male KW - Cell Transformation, Neoplastic KW - Liver Neoplasms, Experimental -- genetics KW - Transfection KW - Liver Neoplasms, Experimental -- chemically induced KW - DNA, Neoplasm -- genetics KW - Proto-Oncogenes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78081994?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Detection+of+activated+proto-oncogenes+in+N-nitrosodiethylamine-induced+liver+tumors%3A+a+comparison+between+B6C3F1+mice+and+Fischer+344+rats.&rft.au=Stowers%2C+S+J%3BWiseman%2C+R+W%3BWard%2C+J+M%3BMiller%2C+E+C%3BMiller%2C+J+A%3BAnderson%2C+M+W%3BEva%2C+A&rft.aulast=Stowers&rft.aufirst=S&rft.date=1988-02-01&rft.volume=9&rft.issue=2&rft.spage=271&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-02 N1 - Date created - 1988-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Circulating glycosaminoglycan anticoagulants associated with suramin treatment. AN - 78080404; 3337895 AB - A complex coagulopathy appeared in three women receiving suramin as treatment for metastatic adrenocortical carcinoma. Although hepatocellular dysfunction accounted for some of the abnormality, a unique feature of the coagulopathy was the presence of an inhibitor of the thrombin clotting time. The potency of this circulating anticoagulant increased markedly during exacerbations of hepatic injury. The anticoagulant was removed from plasma samples from two of the patients by passage over a column of diethylaminoethyl (DEAE)-Sephacel. It eluted from the DEAE at salt concentrations that removed "high-charge" glycosaminoglycans. Elimination of the purified anticoagulant activity in vitro required a combination of heparitinase and chondroitinase ABC, suggesting that the activity was mediated by both heparan sulfate and dermatan sulfate. Suramin is hypothesized to inhibit enzymes that normally degrade glycosaminoglycans, resulting in accumulation of these substances, which are released from the liver into the circulation during periods of hepatic injury. JF - Blood AU - Horne, M K AU - Stein, C A AU - LaRocca, R V AU - Myers, C E AD - Clinical Pathology Department, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/02// PY - 1988 DA - February 1988 SP - 273 EP - 279 VL - 71 IS - 2 SN - 0006-4971, 0006-4971 KW - Anticoagulants KW - 0 KW - Glycosaminoglycans KW - Suramin KW - 6032D45BEM KW - Abridged Index Medicus KW - Index Medicus KW - Liver Diseases -- blood KW - Electrophoresis KW - Chromatography, DEAE-Cellulose KW - Humans KW - Blood Coagulation -- drug effects KW - In Vitro Techniques KW - Adult KW - Female KW - Glycosaminoglycans -- pharmacology KW - Blood Coagulation Disorders -- chemically induced KW - Suramin -- adverse effects KW - Anticoagulants -- blood KW - Glycosaminoglycans -- blood KW - Suramin -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78080404?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Circulating+glycosaminoglycan+anticoagulants+associated+with+suramin+treatment.&rft.au=Horne%2C+M+K%3BStein%2C+C+A%3BLaRocca%2C+R+V%3BMyers%2C+C+E&rft.aulast=Horne&rft.aufirst=M&rft.date=1988-02-01&rft.volume=71&rft.issue=2&rft.spage=273&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-04 N1 - Date created - 1988-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Reduction of doxorubicin cytotoxicity by ouabain: correlation with topoisomerase-induced DNA strand breakage in human and hamster cells. AN - 78034422; 2825982 AB - The cardiac glycoside ouabain, which is a specific inhibitor of the Na+,K+-pump, confers dramatic protection from the cytotoxic effects of doxorubicin (Adriamycin). This effect was documented in cultured A549 cells (human lung adenocarcinoma). CCL210 cells (human fibroblasts), and V79 cells (hamster fibroblasts). Maximum protection from doxorubicin cytotoxicity was achieved using 1 microM ouabain for A549 and CCL210 cells and 300 microM ouabain for V79 cells. These concentrations correlated well with the concentrations of ouabain required to induce Na+,K+-pump blockade, which was assessed using the K+ analogue 86Rb+. This suggests that protection is mediated by pump blockade. Addition of ouabain at the same time as doxorubicin was just as protective as preincubation with ouabain for an hour, demonstrating that the ouabain acts rapidly. Ouabain treatment affected neither influx nor efflux of doxorubicin. Ouabain also had no effect on verapamil-induced inhibition of doxorubicin efflux. However, ouabain partially blocked the verapamil-induced potentiation of the cytotoxic effects of doxorubicin. Therefore, ouabain does not protect by affecting intracellular doxorubicin levels. Fluorescence microscopy showed that the ability of doxorubicin to reach the nucleus was not influenced by ouabain. Alkaline elution studies demonstrated that ouabain greatly decreased doxorubicin-induced DNA strand breakage. Protection from cytotoxicity correlated closely with this decrease in strand breakage. These studies suggest that the stabilization of DNA-topoisomerase II complexes is closely linked to the mechanism of doxorubicin cytotoxicity and that this stabilization is influenced by the intracellular ionic milieu. JF - Cancer research AU - Lawrence, T S AD - Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 725 EP - 730 VL - 48 IS - 3 SN - 0008-5472, 0008-5472 KW - Ouabain KW - 5ACL011P69 KW - Doxorubicin KW - 80168379AG KW - DNA KW - 9007-49-2 KW - DNA Topoisomerases, Type II KW - EC 5.99.1.3 KW - Index Medicus KW - Animals KW - Tumor Cells, Cultured KW - Cell Survival -- drug effects KW - Humans KW - DNA -- metabolism KW - Cell Line KW - Cricetinae KW - Biological Transport -- drug effects KW - DNA Damage KW - Doxorubicin -- antagonists & inhibitors KW - DNA Topoisomerases, Type II -- metabolism KW - Doxorubicin -- toxicity KW - Ouabain -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78034422?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Reduction+of+doxorubicin+cytotoxicity+by+ouabain%3A+correlation+with+topoisomerase-induced+DNA+strand+breakage+in+human+and+hamster+cells.&rft.au=Lawrence%2C+T+S&rft.aulast=Lawrence&rft.aufirst=T&rft.date=1988-02-01&rft.volume=48&rft.issue=3&rft.spage=725&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cytogenetic changes in rat tracheal epithelial cells during early stages of carcinogen-induced neoplastic progression. AN - 78033358; 3335032 AB - The cytogenetic changes in enhanced growth (EG) variants of rat tracheal epithelial cells in culture were examined. These variants which are detectable at 35 days after carcinogen exposure are the first phenotypic alteration in the multistep neoplastic process studied in this model system. Karyotypic analysis of N-methyl-N'-nitro-N-nitrosoguanidine-induced EG variants at Day 35 was made possible by the development of an in situ method of cytogenetic analysis on intact colonies containing too few cells for conventional chromosome preparation methods. Of the transformed EG variant colonies in both control and N-methyl-N'-nitro-N-nitrosoguanidine-treated groups, 62-78% had abnormal karyotypes which included numerical and structural changes. There were no specific chromosome changes, although aberrations of chromosomes 3 and 4 were recurrently observed. However, some colonies of even the most morphologically transformed EG variants were composed of only diploid cells. To confirm this finding 10 EG variant colonies were bisected and half of the clone was prepared for chromosome analysis and the other half was subcultured to measure the clonogenicity and karyotypes of the cells. Cells from 3 colonies plated very poorly on 3T3 feeders and therefore no karyotypic analysis of the colony-forming cells was possible; the cells of the 3 parental colonies were diploid. Three other parental colonies were predominantly diploid (80-90%) but upon replating the resultant daughter colonies had progressively smaller fractions of diploid cells indicating a selection for cells with abnormal karyotypes. When more selective conditions were used (i.e., growth after removal of the feeder cells), the percentage of abnormal cells increased even further. In one case the parental cells had a karyotypic alteration in the long arm of chromosome 4 and this karyotypic alteration was accentuated in the daughter colonies. Thus, selection of cells with increased growth ability upon subculturing or growth in the absence of feeder cells (properties associated with the acquisition of immortality) resulted in concomitant selection for cells with abnormal karyotypes. Since some of the carcinogen-induced rat tracheal epithelial cells expressing the EG variant phenotype were diploid, it is possible that the first step in this transformation process is an epigenetic change. However, most of the diploid cells became terminal. The aneuploid subpopulations present in these colonies have a selective growth advantage and comprise the cell compartment that expresses continued growth, immortality, and ultimately tumorigenicity. JF - Cancer research AU - Oshimura, M AU - Fitzgerald, D J AU - Kitamura, H AU - Nettesheim, P AU - Barrett, J C AD - Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 702 EP - 708 VL - 48 IS - 3 SN - 0008-5472, 0008-5472 KW - Methylnitronitrosoguanidine KW - 12H3O2UGSF KW - Index Medicus KW - Rats KW - Karyotyping KW - Animals KW - Aneuploidy KW - Chromosome Banding KW - Neoplastic Stem Cells -- pathology KW - In Vitro Techniques KW - Cell Transformation, Neoplastic -- drug effects KW - Epithelium -- pathology KW - Time Factors KW - Trachea -- pathology KW - Chromosome Aberrations KW - Tumor Cells, Cultured -- pathology KW - Precancerous Conditions -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78033358?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Cytogenetic+changes+in+rat+tracheal+epithelial+cells+during+early+stages+of+carcinogen-induced+neoplastic+progression.&rft.au=Oshimura%2C+M%3BFitzgerald%2C+D+J%3BKitamura%2C+H%3BNettesheim%2C+P%3BBarrett%2C+J+C&rft.aulast=Oshimura&rft.aufirst=M&rft.date=1988-02-01&rft.volume=48&rft.issue=3&rft.spage=702&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Decontamination and disposal of nitrosoureas and related N-nitroso compounds. AN - 78021504; 3335019 AB - An improved procedure for chemically decontaminating residues of nitrosoureas and related N-nitroso compounds ("nitrosamides") commonly used in the cancer research laboratory is proposed. Treatment of accumulated wastes with aluminum:nickel alloy powder while progressively increasing the basicity of the medium consistently led to at least 99.98% destruction of each nitrosamide tested. Hazardous diazoalkanes were never detected in yields of greater than 0.1%. The mutagenicity of the completed reaction mixtures was never more than 3 times background except when the N-nitroso compound contained a 2-chloroethyl group. In most cases, the completeness of reaction could be determined chromatographically, not only to demonstrate the disappearance of the starting N-nitroso compound, but also to follow production of identifiable products in sufficient abundance to account for the starting material destroyed; none of the organic products observed was mutagenic in any of the four tester strains used. The procedure described herein proved reliable in two checker laboratories besides our own when applied to mixtures of seven N-nitroso compounds: N-methyl-N-nitroso-p-toluene-sulfonamide; N-methyl-N-nitrosourethane; N-methyl-N-nitrosourea; N-methyl-N'-nitro-N-nitrosoguanidine; N-ethyl-N-nitrosourea; N-ethyl-N'-nitro-N-nitrosoguanidine; and N-ethyl-N-nitrosourethane. All of the other procedures investigated for destruction of nitrosamides, including the widely used approach of dissolving the nitrosamides in alkali, were associated with important disadvantages. JF - Cancer research AU - Lunn, G AU - Sansone, E B AU - Andrews, A W AU - Keefer, L K AD - Environmental Control and Research Program, National Cancer Institute, Frederick Cancer Research Facility, Maryland 21701. Y1 - 1988/02/01/ PY - 1988 DA - 1988 Feb 01 SP - 522 EP - 526 VL - 48 IS - 3 SN - 0008-5472, 0008-5472 KW - Hazardous Waste KW - 0 KW - Nitroso Compounds KW - Nitrosourea Compounds KW - Index Medicus KW - Environmental Pollution -- prevention & control KW - Chemistry KW - Chemical Phenomena UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78021504?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Decontamination+and+disposal+of+nitrosoureas+and+related+N-nitroso+compounds.&rft.au=Lunn%2C+G%3BSansone%2C+E+B%3BAndrews%2C+A+W%3BKeefer%2C+L+K&rft.aulast=Lunn&rft.aufirst=G&rft.date=1988-02-01&rft.volume=48&rft.issue=3&rft.spage=522&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Human immunodeficiency virus infection among patients attending clinics for sexually transmitted diseases. AN - 78076266; 3336411 AB - To assess the prevalence and associated risk factors for human immunodeficiency virus (HIV) infection in patients attending inner-city clinics for sexually transmitted diseases in Baltimore, we screened 4028 patients anonymously, of whom 209 (5.2 percent) were seropositive for HIV. HIV-seropositivity rates were higher among men (6.3 percent) than women (3.0 percent) (P less than 0.001) and among blacks (5.0 percent) than whites (1.2 percent) (P less than 0.02). Among men, but not women, HIV seroprevalence increased markedly and steadily up to the age of 40. In men, HIV seropositivity was independently associated with increased age, black race, a history of homosexual contact, and the use of parenteral drugs. In women, a history of parenteral drug use or of being a sexual partner of a bisexual man or parenteral drug user were independently predictive of HIV seropositivity. In men, HIV seropositivity was also associated with a history of syphilis or a reactive serologic test for syphilis, and in women, with a history of genital warts. Since these associations were independent of the type and number of reported sexual partners, they raise the possibility that sexually transmitted diseases that disrupt epithelial surfaces may be important in the transmissibility of HIV. In addition, on a self-administered questionnaire, one third of HIV-infected men and one half of infected women did not acknowledge previous high-risk behavior for HIV exposure. These data suggest that patients at clinics for sexually transmitted diseases represent a group at high risk for HIV infection, and that screening, counseling, and intensive education should be offered to all patients attending such clinics. JF - The New England journal of medicine AU - Quinn, T C AU - Glasser, D AU - Cannon, R O AU - Matuszak, D L AU - Dunning, R W AU - Kline, R L AU - Campbell, C H AU - Israel, E AU - Fauci, A S AU - Hook, E W AD - Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Md. Y1 - 1988/01/28/ PY - 1988 DA - 1988 Jan 28 SP - 197 EP - 203 VL - 318 IS - 4 SN - 0028-4793, 0028-4793 KW - Abridged Index Medicus KW - Index Medicus KW - Population KW - AIDS/HIV KW - United States KW - Measurement KW - Laboratory Examinations And Diagnoses KW - Programs KW - Research Methodology KW - Sex Behavior KW - Organization And Administration KW - Health KW - Delivery Of Health Care KW - Correlation Studies KW - Developed Countries KW - Population At Risk KW - Hiv Infections KW - Population Characteristics KW - Acquired Immunodeficiency Syndrome--prevention and control KW - Demographic Factors KW - Maryland KW - Diseases KW - Data Analysis KW - Infections KW - Health Facilities KW - Reproductive Tract Infections KW - North America KW - Americas KW - Statistical Studies KW - Research Report KW - Studies KW - Program Activities KW - Outpatient Clinic KW - Physical Examinations And Diagnoses KW - Northern America KW - Behavior KW - Bacterial And Fungal Diseases KW - Viral Diseases KW - Risk Factors KW - Sexually Transmitted Diseases KW - Incidence KW - Examinations And Diagnoses KW - Developing Countries KW - Clinic Activities KW - Prevalence KW - Biology KW - Sex Factors KW - Humans KW - African Americans KW - Aged KW - Child KW - Homosexuality KW - Sexual Behavior KW - Acquired Immunodeficiency Syndrome -- transmission KW - Adult KW - Substance-Related Disorders KW - Middle Aged KW - Adolescent KW - Female KW - Male KW - Sexually Transmitted Diseases -- complications KW - HIV Seropositivity -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78076266?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Human+immunodeficiency+virus+infection+among+patients+attending+clinics+for+sexually+transmitted+diseases.&rft.au=Quinn%2C+T+C%3BGlasser%2C+D%3BCannon%2C+R+O%3BMatuszak%2C+D+L%3BDunning%2C+R+W%3BKline%2C+R+L%3BCampbell%2C+C+H%3BIsrael%2C+E%3BFauci%2C+A+S%3BHook%2C+E+W&rft.aulast=Quinn&rft.aufirst=T&rft.date=1988-01-28&rft.volume=318&rft.issue=4&rft.spage=197&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-20 N1 - Date created - 1988-02-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The MAK11 protein is essential for cell growth and replication of M double-stranded RNA and is apparently a membrane-associated protein. AN - 78048179; 2826479 AB - MAK11 is a gene necessary for the maintenance of killer M1 double-stranded RNA, but not for other cellular double-stranded RNAs (L-A, L-BC, T, W). The DNA sequence of this gene revealed a 1407-base pair open reading frame, which corresponds to a 54-kDa protein. The C-terminal region is lysine-rich and is necessary for mak11-complementing activity. The N-terminal 24 amino acids of the open reading frame include 16 hydrophobic amino acids, 4 basic residues, and 4 neutral amino acids; this sequence could span a membrane. We constructed a MAK11-lacZ fusion that includes the entire MAK11 protein and complements the mak11-1 mutation. The fusion protein was localized in a membrane fraction as shown by centrifugation in Percoll gradients. The fusion protein could be released from the membrane fraction by salt washing. Western blotting of protein, isolated from the membrane fraction and purified by p-aminophenyl-beta-D-thiogalactoside-agarose column chromatography, revealed a fusion protein monomer of 170 kDa which agrees with the predicted molecular weight. While the mak11-1 mutation results in specific loss of M1 double-stranded RNA without any apparent growth defect, replacing a 792-base pair internal EcoRV fragment of MAK11 with the URA3 gene (gene disruption) resulted in a lethal mutation. JF - The Journal of biological chemistry AU - Icho, T AU - Wickner, R B AD - Section on Genetics of Simple Eukaryotes, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892. Y1 - 1988/01/25/ PY - 1988 DA - 1988 Jan 25 SP - 1467 EP - 1475 VL - 263 IS - 3 SN - 0021-9258, 0021-9258 KW - Fungal Proteins KW - 0 KW - KHR1 protein, S cerevisiae KW - Killer Factors, Yeast KW - Membrane Glycoproteins KW - Mycotoxins KW - RNA, Double-Stranded KW - RNA, Fungal KW - Recombinant Fusion Proteins KW - Saccharomyces cerevisiae Proteins KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Index Medicus KW - Saccharomyces cerevisiae -- genetics KW - Recombinant Fusion Proteins -- analysis KW - Base Sequence KW - DNA Restriction Enzymes -- metabolism KW - RNA, Fungal -- biosynthesis KW - Recombinant Fusion Proteins -- isolation & purification KW - RNA, Double-Stranded -- biosynthesis KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Cloning, Molecular KW - Mycotoxins -- genetics KW - Membrane Glycoproteins -- analysis KW - Fungal Proteins -- genetics KW - Fungal Proteins -- analysis KW - Mycotoxins -- analysis KW - Membrane Glycoproteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78048179?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=The+MAK11+protein+is+essential+for+cell+growth+and+replication+of+M+double-stranded+RNA+and+is+apparently+a+membrane-associated+protein.&rft.au=Icho%2C+T%3BWickner%2C+R+B&rft.aulast=Icho&rft.aufirst=T&rft.date=1988-01-25&rft.volume=263&rft.issue=3&rft.spage=1467&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-25 N1 - Date created - 1988-02-25 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - J03506; GENBANK N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Deletional analysis of the promoter region of the human transferrin receptor gene. AN - 19626128; 8740168 AB - Fragments of human genomic DNA corresponding to the promoter region of the gene for the transferrin receptor have been cloned upstream of the bacterial gene for chloramphenicol acetyltransferase and these constructs used to assess promoter activity following transfection into a human rhabdomyosarcoma cell line. Progressive 5' deletions as well as internal linker-substitution constructs support a critical role in gene expression of a sequence element approximately 70 bp upstream of the mRNA start site. In this region, the receptor gene was found to contain 11bp that are identical to a segment of the enhancers of polyoma virus and adenovirus. A fragment encompassing this element was shown to increase gene expression when the fragment was placed in either orientation upstream of the remainder of the transferrin receptor promoter but the same fragment did not activate an enhancer-less SV40 promoter. Removal from within the receptor promoter of three potential binding sites for the transcription factor Sp1 did not decrease the promoter's activity. Images JF - Nucleic Acids Research AU - Casey, J L AU - Di Jeso, B AU - Rao, K K AU - Rouault, T A AU - Klausner, R D AU - Harford, J B AD - Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892. Y1 - 1988/01/25/ PY - 1988 DA - 1988 Jan 25 SP - 629 EP - 646 PB - Oxford University Press, Oxford Journals, Great Clarendon Street VL - 16 IS - 2 SN - 0305-1048, 0305-1048 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts; Virology & AIDS Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Adenovirus KW - Polyomavirus KW - Gene expression KW - Promoters KW - Sp1 protein KW - Enhancers KW - Chloramphenicol O-acetyltransferase KW - Gene deletion KW - Transferrin receptors KW - Transfection KW - Transcription factors KW - Simian virus 40 KW - DNA KW - genomics KW - Rhabdomyosarcoma KW - J 02310:Genetics & Taxonomy KW - N 14830:RNA KW - V 22310:Genetics, Taxonomy & Structure KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19626128?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=Deletional+analysis+of+the+promoter+region+of+the+human+transferrin+receptor+gene.&rft.au=Casey%2C+J+L%3BDi+Jeso%2C+B%3BRao%2C+K+K%3BRouault%2C+T+A%3BKlausner%2C+R+D%3BHarford%2C+J+B&rft.aulast=Casey&rft.aufirst=J&rft.date=1988-01-25&rft.volume=16&rft.issue=2&rft.spage=629&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Gene expression; Enhancers; Sp1 protein; Promoters; Gene deletion; Chloramphenicol O-acetyltransferase; Transferrin receptors; Transfection; Transcription factors; DNA; genomics; Rhabdomyosarcoma; Simian virus 40; Adenovirus; Polyomavirus ER - TY - JOUR T1 - Differences in platelet enzyme activity between alcoholics and nonalcoholics. AN - 78085462; 3336400 AB - Blood platelets are an accessible tissue that reflects the activity of many enzymes found in the brain. To investigate the possible effect on such enzymes of long-term consumption of large quantities of ethanol, we assayed the activities of two enzymes, monoamine oxidase and adenylate cyclase, in platelet membranes of men with alcoholism and controls matched for sex and age. We also compared these two groups in terms of the inhibition of platelet monoamine oxidase activity by ethanol in vitro (400 mM), and in terms of the stimulation of adenylate cyclase activity by various agents. There was no significant difference in monoamine oxidase activity between the alcoholics and the controls. However, the inhibition of monoamine oxidase by ethanol was significantly higher in the platelets of alcoholics. The basal activity of adenylate cyclase was the same in platelets from the alcoholics and the controls, but the platelet adenylate cyclase activity after stimulation with guanine nucleotide, cesium fluoride, or prostaglandin E1 was significantly lower in alcoholics. These differences were not associated with age, race, smoking, or illicit drug use, and there was no significant correlation with the duration of problems with alcohol. The changes were long-lasting; cesium fluoride-stimulated adenylate cyclase activity was lower in alcoholic subjects who had abstained from alcohol for one to four years. Discriminant analysis showed that the use of values for the inhibition of monoamine oxidase activity by ethanol and cesium fluoride-stimulated adenylate cyclase activity correctly classified 75 percent of the alcoholics and 73 percent of the controls. These measures may be of value either as indexes of excessive alcohol consumption or as an indication of a predisposition to alcoholism. JF - The New England journal of medicine AU - Tabakoff, B AU - Hoffman, P L AU - Lee, J M AU - Saito, T AU - Willard, B AU - De Leon-Jones, F AD - National Institute on Alcohol Abuse and Alcoholism, Bethesda, Md 20892. Y1 - 1988/01/21/ PY - 1988 DA - 1988 Jan 21 SP - 134 EP - 139 VL - 318 IS - 3 SN - 0028-4793, 0028-4793 KW - Adenylyl Cyclase Inhibitors KW - 0 KW - Monoamine Oxidase Inhibitors KW - Cesium KW - 1KSV9V4Y4I KW - Ethanol KW - 3K9958V90M KW - Monoamine Oxidase KW - EC 1.4.3.4 KW - Adenylyl Cyclases KW - EC 4.6.1.1 KW - Fluorides KW - Q80VPU408O KW - cesium fluoride KW - T76A371HJR KW - Abridged Index Medicus KW - Index Medicus KW - Cesium -- pharmacology KW - Smoking -- blood KW - Cell Membrane -- enzymology KW - Ethanol -- pharmacology KW - Humans KW - Continental Population Groups KW - Adult KW - Time Factors KW - Monoamine Oxidase Inhibitors -- pharmacology KW - Male KW - Alcoholism -- enzymology KW - Blood Platelets -- enzymology KW - Adenylyl Cyclases -- blood KW - Monoamine Oxidase -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78085462?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Differences+in+platelet+enzyme+activity+between+alcoholics+and+nonalcoholics.&rft.au=Tabakoff%2C+B%3BHoffman%2C+P+L%3BLee%2C+J+M%3BSaito%2C+T%3BWillard%2C+B%3BDe+Leon-Jones%2C+F&rft.aulast=Tabakoff&rft.aufirst=B&rft.date=1988-01-21&rft.volume=318&rft.issue=3&rft.spage=134&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-04 N1 - Date created - 1988-02-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Phase I studies of 2',3'-dideoxycytidine in severe human immunodeficiency virus infection as a single agent and alternating with zidovudine (AZT). AN - 78079476; 2891981 AB - Five dose regimens of 2',3'-dideoxycytidine (ddC) were administered, intravenously for 2 weeks then orally for 4 or more weeks, to 20 patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC). ddC was well absorbed from the gut and crossed the blood-brain barrier. 10 of the 15 patients who received 0.03-0.09 mg/kg every 4 h had increases in their absolute number of T4+ T cells at week 2 (p less than 0.05), though in many these rises were not sustained. 11 of 13 evaluable patients had a fall in their serum human immunodeficiency virus (HIV)p24 antigen by week 2 of therapy (p less than 0.01); in 4 patients the p24 antigen subsequently rose to baseline while in others the decline was sustained. Dose-related toxic effects included cutaneous eruptions, fever, mouth sores, thrombocytopenia, and neutropenia. A reversible painful peripheral neuropathy developed in 10 patients after 6-14 weeks' treatment. These results suggest that ddC has activity against HIV in vivo and has a different toxicity profile from that of zidovudine (AZT). 6 patients with AIDS or ARC were given an alternating regimen of oral AZT (200 mg every 4 h for 7 days) and oral ddC (0.03 mg/kg every 4 h for 7 days). The regimen was well tolerated, and the 5 patients who completed 9 or more weeks of treatment had sustained rises in their T4+ T cells and/or falls in p24 antigen. JF - Lancet (London, England) AU - Yarchoan, R AU - Perno, C F AU - Thomas, R V AU - Klecker, R W AU - Allain, J P AU - Wills, R J AU - McAtee, N AU - Fischl, M A AU - Dubinsky, R AU - McNeely, M C AD - National Cancer Institute, Bethesda, Maryland. Y1 - 1988/01/16/ PY - 1988 DA - 1988 Jan 16 SP - 76 EP - 81 VL - 1 IS - 8577 SN - 0140-6736, 0140-6736 KW - Deoxycytidine KW - 0W860991D6 KW - Zidovudine KW - 4B9XT59T7S KW - Zalcitabine KW - 6L3XT8CB3I KW - Thymidine KW - VC2W18DGKR KW - Abridged Index Medicus KW - Index Medicus KW - AIDS/HIV KW - Drug Therapy, Combination KW - HIV -- drug effects KW - Drug Evaluation KW - Drug Administration Schedule KW - Humans KW - Adult KW - Middle Aged KW - Male KW - Thymidine -- administration & dosage KW - Deoxycytidine -- analogs & derivatives KW - AIDS-Related Complex -- drug therapy KW - Deoxycytidine -- therapeutic use KW - Deoxycytidine -- administration & dosage KW - Acquired Immunodeficiency Syndrome -- drug therapy KW - Thymidine -- therapeutic use KW - Thymidine -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78079476?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medicinal+chemistry&rft.atitle=Molecular+complexes+of+thyroid+hormone+tyrosyl+rings+with+aromatic+donors.+Possible+relationship+to+receptor+protein+interactions.&rft.au=Chae%2C+K%3BMcKinney%2C+J+D&rft.aulast=Chae&rft.aufirst=K&rft.date=1988-02-01&rft.volume=31&rft.issue=2&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Journal+of+medicinal+chemistry&rft.issn=00222623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-17 N1 - Date created - 1988-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Failure to synthesize the T cell CD3-zeta chain: structure and function of a partial T cell receptor complex. AN - 78116913; 3278811 AB - The T cell antigen receptor is composed of two variable chains (alpha and beta, termed Ti), which confer ligand specificity, and five constant chains (gamma, delta, epsilon, zeta, and p21, collectively termed CD3) whose functions are poorly understood. To explore the roles of the individual CD3 components, an antigen-specific murine T cell hybridoma was chemically mutagenized and antigen-induced growth inhibition was used to select CD3/Ti expression variants. One variant produced all CD3/Ti components except CD3-zeta and was able to express small amounts of surface CD3/Ti. This variant failed to respond normally to either antigen or a mitogenic anti-Thy-1 antibody. Surprisingly, in the absence of CD3-zeta, direct cross-linking of the partial receptor induced both phosphatidylinositol hydrolysis and interleukin 2 production. These data indicate that CD3-zeta determines the normal intracellular fate of the T cell antigen receptor and is likely to play an important role in physiologically relevant transmembrane signaling. JF - Cell AU - Sussman, J J AU - Bonifacino, J S AU - Lippincott-Schwartz, J AU - Weissman, A M AU - Saito, T AU - Klausner, R D AU - Ashwell, J D AD - Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/15/ PY - 1988 DA - 1988 Jan 15 SP - 85 EP - 95 VL - 52 IS - 1 SN - 0092-8674, 0092-8674 KW - Antibodies, Monoclonal KW - 0 KW - Interleukin-2 KW - Phosphatidylinositols KW - Receptors, Antigen, T-Cell KW - Index Medicus KW - Phosphatidylinositols -- metabolism KW - Animals KW - Hybridomas KW - Interleukin-2 -- biosynthesis KW - Mice KW - Cell Membrane -- metabolism KW - Hydrolysis KW - Fluorescent Antibody Technique KW - Immunoassay KW - Antibodies, Monoclonal -- immunology KW - T-Lymphocytes -- physiology KW - Receptors, Antigen, T-Cell -- biosynthesis KW - Receptors, Antigen, T-Cell -- analysis KW - T-Lymphocytes -- immunology KW - Receptors, Antigen, T-Cell -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78116913?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell&rft.atitle=Failure+to+synthesize+the+T+cell+CD3-zeta+chain%3A+structure+and+function+of+a+partial+T+cell+receptor+complex.&rft.au=Sussman%2C+J+J%3BBonifacino%2C+J+S%3BLippincott-Schwartz%2C+J%3BWeissman%2C+A+M%3BSaito%2C+T%3BKlausner%2C+R+D%3BAshwell%2C+J+D&rft.aulast=Sussman&rft.aufirst=J&rft.date=1988-01-15&rft.volume=52&rft.issue=1&rft.spage=85&rft.isbn=&rft.btitle=&rft.title=Cell&rft.issn=00928674&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-14 N1 - Date created - 1988-04-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Role of metabolism and DNA adduct formation in the induction of sister chromatid exchanges in human lymphocytes by diethylstilbestrol. AN - 78039015; 3335009 AB - Recent studies have shown that diethylstilbestrol (DES) induces sister chromatid exchanges (SCEs) in lymphocytes from pregnant and premenopausal women but has only a slight effect on lymphocytes from post-menopausal women or men. In this study blood specimens from premenopausal women were used to define the role of different metabolic pathways on DES-induced formation of SCEs and to determine whether conditions resulting in induction of SCEs also induced detectable levels of DNA adducts. Exposure of lymphocytes in vitro to 0-40 microM DES induced a concentration-dependent increase in SCEs. Addition of indomethacin to the cultures partially abolished DES-induced SCEs, suggesting involvement of prostaglandin synthetases in the formation of specific DES metabolites that cause SCEs. alpha-Naphthoflavone, an inhibitor of cytochrome P-450 monooxygenases, had no effect on DES-induced SCEs. Cells exposed to DES at doses sufficient to cause large increases in SCE induction did not have adducts detectable by a 32P-postlabeling assay capable of revealing adducts at a level of 1 adduct/10(9) normal nucleotides. JF - Cancer research AU - Lundgren, K AU - Randerath, K AU - Everson, R B AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/01/15/ PY - 1988 DA - 1988 Jan 15 SP - 335 EP - 338 VL - 48 IS - 2 SN - 0008-5472, 0008-5472 KW - Benzoflavones KW - 0 KW - alpha-naphthoflavone KW - 604-59-1 KW - Diethylstilbestrol KW - 731DCA35BT KW - DNA KW - 9007-49-2 KW - Indomethacin KW - XXE1CET956 KW - Index Medicus KW - Dose-Response Relationship, Drug KW - Humans KW - Lymphocytes -- ultrastructure KW - Lymphocytes -- drug effects KW - Female KW - Indomethacin -- pharmacology KW - Benzoflavones -- pharmacology KW - Diethylstilbestrol -- metabolism KW - Diethylstilbestrol -- toxicity KW - DNA -- metabolism KW - Sister Chromatid Exchange -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78039015?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Role+of+metabolism+and+DNA+adduct+formation+in+the+induction+of+sister+chromatid+exchanges+in+human+lymphocytes+by+diethylstilbestrol.&rft.au=Lundgren%2C+K%3BRanderath%2C+K%3BEverson%2C+R+B&rft.aulast=Lundgren&rft.aufirst=K&rft.date=1988-01-15&rft.volume=48&rft.issue=2&rft.spage=335&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Contrasting duration of inhibition of cell-cell communication in primary mouse epidermal cells by phorbol 12,13-dibutyrate and by bryostatin 1. AN - 78034214; 3422054 AB - The bryostatins, macrocyclic lactones isolated on the basis of their antineoplastic activity, activate protein kinase C in vitro and inhibit phorbol ester binding to the enzyme. In intact cells, the bryostatins induce some phorbol ester responses, such as neutrophil activation, but paradoxically they not only fail to induce other responses, e.g., differentiation in HL-60 promyelocytic leukemia cells, but actually block response to the phorbol esters. We compare here bryostatin I and phorbol 12,13-dibutyrate as inhibitors of cell-cell communication in cultured primary mouse epidermal cells. Like phorbol 12,13-dibutyrate, bryostatin I at nanomolar concentrations markedly inhibited cell coupling. It differed from the phorbol esters, however, in that its action was more transient. By 4 h of incubation bryostatin 1 caused little inhibition of coupling. Moreover, coincubation of bryostatin 1 and phorbol 12,13-dibutyrate gave no greater response at this time than that found for bryostatin 1 alone. Time-dependent inhibition of the protein kinase C pathway could account for many of the observed differences between the actions of the phorbol esters and bryostatin 1. JF - Cancer research AU - Pasti, G AU - Rivedal, E AU - Yuspa, S H AU - Herald, C L AU - Pettit, G R AU - Blumberg, P M AD - Molecular Mechanisms of Tumor Promotion Section, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/15/ PY - 1988 DA - 1988 Jan 15 SP - 447 EP - 451 VL - 48 IS - 2 SN - 0008-5472, 0008-5472 KW - Bryostatins KW - 0 KW - Lactones KW - Macrolides KW - Phorbol Esters KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - bryostatin 1 KW - 37O2X55Y9E KW - Protein Kinase C KW - EC 2.7.11.13 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Skin -- drug effects KW - Dose-Response Relationship, Drug KW - Cells, Cultured KW - Calcium -- physiology KW - Mice KW - Protein Kinase C -- physiology KW - Mice, Inbred BALB C KW - Time Factors KW - Phorbol Esters -- pharmacology KW - Cell Communication -- drug effects KW - Lactones -- metabolism KW - Lactones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78034214?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Contrasting+duration+of+inhibition+of+cell-cell+communication+in+primary+mouse+epidermal+cells+by+phorbol+12%2C13-dibutyrate+and+by+bryostatin+1.&rft.au=Pasti%2C+G%3BRivedal%2C+E%3BYuspa%2C+S+H%3BHerald%2C+C+L%3BPettit%2C+G+R%3BBlumberg%2C+P+M&rft.aulast=Pasti&rft.aufirst=G&rft.date=1988-01-15&rft.volume=48&rft.issue=2&rft.spage=447&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Risk of second cancers after treatment for Hodgkin's disease. AN - 78079555; 3336397 AB - We estimated the risk of second cancers among 1507 patients with Hodgkin's disease treated at Stanford University Medical Center since 1968. Eight-three second cancers occurred more than one year after diagnosis, as compared with 15.9 expected on the basis of rates in the general population (relative risk, 5.2; 95 percent confidence interval, 4.2 to 6.5). The mean (+/- SE) 15-year actuarial risk of all second cancers was 17.6 +/- 3.1 percent, of which 13.2 +/- 3.1 percent was due to solid tumors. The risk of leukemia appeared to reach a plateau level of 3.3 +/- 0.6 percent at 10 years, whereas non-Hodgkin's lymphoma continued to increase, to 1.6 +/- 0.7 percent by the end of the follow-up period. The risk of solid tumors did not vary significantly according to treatment category, with the array of neoplasms resembling that previously observed in populations exposed to radiation and in immunosuppressed groups. The risk of leukemia, although elevated after radiation therapy alone (relative risk, 11; 95 percent confidence interval, 1.2 to 38), was much higher after either adjuvant chemotherapy (relative risk, 117; 95 percent confidence interval, 69 to 185) or chemotherapy alone (relative risk, 130; 95 percent confidence interval, 26 to 380). These data suggest that the risk of solid tumors after therapy for Hodgkin's disease continues to increase with time. JF - The New England journal of medicine AU - Tucker, M A AU - Coleman, C N AU - Cox, R S AU - Varghese, A AU - Rosenberg, S A AD - Environmental Epidemiology Branch, National Cancer Institute, Bethesda, MD. 20892. Y1 - 1988/01/14/ PY - 1988 DA - 1988 Jan 14 SP - 76 EP - 81 VL - 318 IS - 2 SN - 0028-4793, 0028-4793 KW - Antineoplastic Agents KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - Leukemia -- chemically induced KW - Neoplasms, Radiation-Induced -- etiology KW - Humans KW - Leukemia, Radiation-Induced -- etiology KW - Aged KW - Child KW - Radiotherapy -- adverse effects KW - Antineoplastic Agents -- adverse effects KW - Child, Preschool KW - Risk Factors KW - Neoplasms -- chemically induced KW - Adult KW - Lymphoma, Non-Hodgkin -- etiology KW - Middle Aged KW - Adolescent KW - Female KW - Neoplasms, Multiple Primary KW - Hodgkin Disease -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78079555?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Risk+of+second+cancers+after+treatment+for+Hodgkin%27s+disease.&rft.au=Tucker%2C+M+A%3BColeman%2C+C+N%3BCox%2C+R+S%3BVarghese%2C+A%3BRosenberg%2C+S+A&rft.aulast=Tucker&rft.aufirst=M&rft.date=1988-01-14&rft.volume=318&rft.issue=2&rft.spage=76&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-02 N1 - Date created - 1988-02-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Modulation of neurotransmitter metabolism by dihydropyridine calcium channel ligands in mouse brain. AN - 78116026; 2449929 AB - The regional concentrations of dopamine, serotonin, dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindole acetic acid were measured in mouse brain following administration of the dihydropyridine calcium channel activator BAY K 8644, and antagonist, nifedipine. BAY K 8644 (1-8 mg/kg) produced dose- and time-dependent increases in dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid concentrations in the caudate, without altering dopamine and serotonin levels. No changes in 5-hydroxyindoleacetic acid concentration were observed in the raphe nuclei, hypothalamus, hippocampus and frontal cortex. Nifedipine (4 mg/kg) blocked BAY K 8644- (2 mg/kg) elicited increases in dihydroxyphenylacetic acid in the caudate. Furthermore, a higher dose of nifedipine (8 mg/kg) decreased dihydroxyphenylacetic acid and homovanillic acid, but did not affect dopamine, serotonin or 5-hydroxyindoleacetic acid concentrations, while a lower dose of nifedipine (2 mg/kg) significantly increased serotonin, 5-hydroxyindoleacetic acid and homovanillic acid, but did not affect dopamine and dihydroxyphenylacetic acid concentrations. The findings that both BAY K 8644 and nifedipine affect neurotransmitter metabolism in vivo in a dose-, time- and brain region-dependent manner, suggest that high-affinity dihydropyridine calcium channel binding sites play an important role in regulating neurotransmitter turnover in the central nervous system. JF - Brain research AU - Bolger, G T AU - Lesieur, P AU - Basile, A S AU - Skolnick, P AD - Laboratory of Neuroscience, NIDDK, Bethesda, MD 20892. Y1 - 1988/01/12/ PY - 1988 DA - 1988 Jan 12 SP - 101 EP - 107 VL - 438 IS - 1-2 SN - 0006-8993, 0006-8993 KW - Dihydropyridines KW - 0 KW - Ion Channels KW - Neurotransmitter Agents KW - 3,4-Dihydroxyphenylacetic Acid KW - 102-32-9 KW - Hydroxyindoleacetic Acid KW - 54-16-0 KW - 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester KW - 71145-03-4 KW - Nifedipine KW - I9ZF7L6G2L KW - Dopamine KW - VTD58H1Z2X KW - Homovanillic Acid KW - X77S6GMS36 KW - Index Medicus KW - Animals KW - Drug Interactions KW - 3,4-Dihydroxyphenylacetic Acid -- metabolism KW - Hydroxyindoleacetic Acid -- metabolism KW - Dopamine -- metabolism KW - Mice KW - Homovanillic Acid -- metabolism KW - Male KW - Nifedipine -- pharmacology KW - Dihydropyridines -- pharmacology KW - Neurotransmitter Agents -- metabolism KW - 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester -- metabolism KW - Brain -- drug effects KW - Ion Channels -- drug effects KW - 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester -- pharmacology KW - Dihydropyridines -- metabolism KW - Nifedipine -- metabolism KW - Brain -- metabolism KW - Ion Channels -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78116026?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research&rft.atitle=Modulation+of+neurotransmitter+metabolism+by+dihydropyridine+calcium+channel+ligands+in+mouse+brain.&rft.au=Bolger%2C+G+T%3BLesieur%2C+P%3BBasile%2C+A+S%3BSkolnick%2C+P&rft.aulast=Bolger&rft.aufirst=G&rft.date=1988-01-12&rft.volume=438&rft.issue=1-2&rft.spage=101&rft.isbn=&rft.btitle=&rft.title=Brain+research&rft.issn=00068993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-18 N1 - Date created - 1988-04-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Monoclonal antibodies against type II rat brain protein kinase C. AN - 78047469; 3422075 AB - Monoclonal antibodies (8/1, 10/10, and 25/3) against rat brain type II protein kinase C were used for the immunochemical characterization of this kinase. These antibodies immunoprecipitated the type II protein kinase C in a dose-dependent manner but did neither to the type I nor III isozyme. Immunoblot analysis of the tryptic fragments from protein kinase C revealed that all three antibodies recognized the 27-38-kDa fragments, the phospholipid/phorbol ester-binding domain, but not the 45-48-kDa fragments, the kinase catalytic domain. The immune complexes of the kinase and the antibodies retained 70-80% of the kinase activity which was dependent on Ca2+ and phosphatidylserine and further activated by diacylglycerol or tumor-promoting phorbol ester. With antibody 8/1, the kinetic parameters with respect to Km for ATP and histone and K alpha for phosphatidylserine and phorbol 12,13-dibutyrate were not significantly influenced. However, the antibody causes variable effects on the K alpha for Ca2+ under different assay conditions. When determined in the presence of phosphatidylserine, the K alpha for Ca2+ was reduced by an order of magnitude (37 +/- 8 to 2.0 +/- 1.8 microM); in the presence of phosphatidylserine and phorbol 12,13-dibutyrate, the K alpha for Ca2+ was not significantly altered; and in the presence of phosphatidylserine and dioleoylglycerol, the kinase became an apparently Ca2+-independent enzyme. The effects of antibody 8/1 on the kinetic parameters of the enzyme for phorbol ester binding were different from those for kinase activity. This antibody causes a 20-30% reduction in phorbol ester binding and a 2-fold increase (1.9 +/- 0.2 to 3.9 +/- 0.3 micrograms/ml) in the concentration of phosphatidylserine required for half-maximal binding, but is without significant influence on those parameters for Ca2+ and phorbol 12,13-dibutyrate. The differential effects of antibody 8/1 on kinase activity and phorbol ester binding with respect to the kinetic parameter of phosphatidylserine suggest that the roles of this phospholipid in supporting phorbol ester binding and kinase activation are different. In the presence of the antibody, the autophosphorylations of the phospholipid/phorbol ester-binding domain and the kinase domain were reduced; the reduction was more pronounced for the former than for the latter. These results suggest that the epitope for antibody 8/1 is localized within the phospholipid/phorbol ester-binding domain at the region adjacent to the kinase domain so that the autophosphorylations of both domains are affected. JF - The Journal of biological chemistry AU - Nakabayashi, H AU - Huang, K P AD - Section on Metabolic Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892. Y1 - 1988/01/05/ PY - 1988 DA - 1988 Jan 05 SP - 298 EP - 304 VL - 263 IS - 1 SN - 0021-9258, 0021-9258 KW - Antibodies, Monoclonal KW - 0 KW - Antigen-Antibody Complex KW - Carcinogens KW - Isoenzymes KW - Peptide Fragments KW - Phorbol Esters KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Rats KW - Phorbol Esters -- pharmacology KW - Carcinogens -- pharmacology KW - Animals KW - Phosphorylation KW - Kinetics KW - Peptide Fragments -- analysis KW - Calcium -- pharmacology KW - Protein Kinase C -- analysis KW - Isoenzymes -- analysis KW - Brain -- enzymology KW - Isoenzymes -- immunology KW - Protein Kinase C -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78047469?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Monoclonal+antibodies+against+type+II+rat+brain+protein+kinase+C.&rft.au=Nakabayashi%2C+H%3BHuang%2C+K+P&rft.aulast=Nakabayashi&rft.aufirst=H&rft.date=1988-01-05&rft.volume=263&rft.issue=1&rft.spage=298&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-17 N1 - Date created - 1988-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Reverse transformation of Harvey murine sarcoma virus-transformed NIH/3T3 cells by site-selective cyclic AMP analogs. AN - 78029291; 2826444 AB - Eighteen site-selective cAMP analogs modified at either the C-8 position or the C-6 position were tested for their growth regulatory effects on the Harvey murine sarcoma virus-transformed NIH/3T3 clone 13-3B-4 cells grown in a serum-free defined medium. All 18 analogs, when tested individually, exhibited an appreciable growth inhibitory effect at micromolar concentrations. The most potent growth inhibitory analogs contained a thio moiety at the C-8 position. In general, C-6 analogs required 5-10-fold greater concentrations than C-8 analogs to produce the same degree of growth inhibition. The growth inhibition induced by these analogs was accompanied by a change in cell morphology; cells treated with the analogs exhibited the morphology characteristic of untransformed fibroblasts, while untreated cells retained a transformed phenotype. The regulatory subunit of cAMP-dependent protein kinase, the cAMP receptor protein, has two different intrachain cAMP binding sites, and cAMP analogs modified at the C-8 position (C-8 analogs) are generally selective for Site 1, while analogs modified at the C-6 position (C-6 analogs) are generally selective for Site 2. Thus, C-8 and C-6 analogs were tested in combination to enhance the growth regulatory effect. Both growth inhibition and morphological change were enhanced synergistically by a combination of the C-6 and C-8 analogs. Two C-6 analogs or two C-8 analogs added together did not cause synergism. For both growth inhibition and phenotypic change, C-8 thio analogs acted far more synergistically than C-8 amino analogs when cells were treated in combination with C-6 analogs, suggesting a response of the RII rather than the RI cAMP receptor protein. DEAE-cellulose chromatography revealed that the growth inhibition, in fact, correlates with an increase of the RII cAMP receptor protein and a decrease of the RI receptor protein. The growth inhibitory effect of the site-selective analogs was not due to the cytotoxic effect of adenosine metabolites as shown by the different behavior of 8-Cl-cAMP compared with 8-Cl-adenosine in 1) cell cycle effects and 2) release from growth inhibition. It is concluded that the observed growth inhibition and phenotypic reversion of 13-3B-4 cells is most likely mediated through the cellular effector, the RII cAMP receptor protein. JF - The Journal of biological chemistry AU - Tagliaferri, P AU - Katsaros, D AU - Clair, T AU - Neckers, L AU - Robins, R K AU - Cho-Chung, Y S AD - Cellular Biochemistry Section, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/05/ PY - 1988 DA - 1988 Jan 05 SP - 409 EP - 416 VL - 263 IS - 1 SN - 0021-9258, 0021-9258 KW - Cyclic AMP KW - E0399OZS9N KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Cells, Cultured KW - Kinetics KW - Mice KW - Drug Synergism KW - Structure-Activity Relationship KW - Cyclic AMP -- pharmacology KW - Cyclic AMP -- analogs & derivatives KW - Harvey murine sarcoma virus -- genetics KW - Cell Transformation, Neoplastic -- drug effects KW - Sarcoma Viruses, Murine -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78029291?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Reverse+transformation+of+Harvey+murine+sarcoma+virus-transformed+NIH%2F3T3+cells+by+site-selective+cyclic+AMP+analogs.&rft.au=Tagliaferri%2C+P%3BKatsaros%2C+D%3BClair%2C+T%3BNeckers%2C+L%3BRobins%2C+R+K%3BCho-Chung%2C+Y+S&rft.aulast=Tagliaferri&rft.aufirst=P&rft.date=1988-01-05&rft.volume=263&rft.issue=1&rft.spage=409&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-17 N1 - Date created - 1988-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neuropsychiatric consequences of AIDS. AN - 85281074; pmid-3348597 AB - As part of a study in progress, neuropsychological tests have been administered to 13 patients with clinical acquired immunodeficiency syndrome, 9 human immunodeficiency virus-seropositive patients, 4 seropositive patients with chronic active hepatitis, 5 seronegative patients with chronic active hepatitis, and 6 healthy controls. Consistent with findings in earlier patient and control groups, the acquired immunodeficiency syndrome patients had substantially lower scores on a variety of cognitive tests. Although the acquired immunodeficiency syndrome group is not education-matched at present, the results are nonetheless consistent with impairments of language function and timed, self-paced performance. Test results obtained may reflect focal and global cognitive impairment as well as motivational decrements in patients with the acquired immunodeficiency syndrome relative to seropositive patients or controls. JF - Annals of Neurology AU - Rubinow, D R AU - Berrettini, C H AU - Brouwers, P AU - Lane, H C AD - Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, MD 20892. PY - 1988 SP - S24 EP - S26 VL - 23 Suppl SN - 0364-5134, 0364-5134 KW - Analysis of Variance KW - Reference Values KW - HIV Seropositivity KW - Mental Disorders KW - Human KW - Acquired Immunodeficiency Syndrome KW - Brain Diseases KW - Neuropsychological Tests KW - Male UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85281074?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+Neurology&rft.atitle=Neuropsychiatric+consequences+of+AIDS.&rft.au=Rubinow%2C+D+R%3BBerrettini%2C+C+H%3BBrouwers%2C+P%3BLane%2C+H+C&rft.aulast=Rubinow&rft.aufirst=D&rft.date=1988-01-01&rft.volume=23+Suppl&rft.issue=&rft.spage=S24&rft.isbn=&rft.btitle=&rft.title=Annals+of+Neurology&rft.issn=03645134&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - The relationship between genetic deafness and fear-related behaviors in nervous pointer dogs. AN - 85261966; pmid-3174842 AB - Nervous pointer dogs have been extensively characterized as an animal model for some human pathological anxiety states. During our work with these animals suspicion developed that some of these dogs had a hearing deficit. We decided to systematically evaluate this observation and to study the relation between hearing status and fear-related behaviors in the nervous pointer dogs. Our results revealed that a majority of the nervous dogs in our colony (75%) suffer from bilateral deafness as demonstrated by complete absence of brain stem auditory evoked response. Furthermore, behavioral ratings revealed that hearing and deaf dogs do not differ in their pathological response to the characteristic fear-provoking stimuli (e.g., human interaction) and that both hearing and deaf nervous dogs markedly differ from normal dogs in that respect. The relation between these abnormalities and their implications for research involving breeding of animals for selected traits are further discussed in this report. JF - Physiology & Behavior AU - Klein, E AU - Steinberg, S A AU - Weiss, S R AU - Matthews, D M AU - Uhde, T W AD - Unit on Anxiety and Affective Disorders, NIMH, Bethesda, MD 20892. PY - 1988 SP - 307 EP - 312 VL - 43 IS - 3 SN - 0031-9384, 0031-9384 KW - Dog Diseases KW - Arousal KW - Fear KW - Animal KW - Disease Models, Animal KW - Behavior, Animal KW - Deafness KW - Brain Stem KW - Dogs KW - Evoked Potentials, Auditory KW - Support, Non-U.S. Gov't KW - Male KW - Female UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85261966?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Physiology+%26+Behavior&rft.atitle=The+relationship+between+genetic+deafness+and+fear-related+behaviors+in+nervous+pointer+dogs.&rft.au=Klein%2C+E%3BSteinberg%2C+S+A%3BWeiss%2C+S+R%3BMatthews%2C+D+M%3BUhde%2C+T+W&rft.aulast=Klein&rft.aufirst=E&rft.date=1988-01-01&rft.volume=43&rft.issue=3&rft.spage=307&rft.isbn=&rft.btitle=&rft.title=Physiology+%26+Behavior&rft.issn=00319384&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - The National Toxicology Program chemical nomination selection and testing process. AN - 78821065; 2980357 AB - The NTP is an interagency program of the Federal Government which coordinates toxicological programs at the NIH (NIEHS), FDA (NCTR), and CDC (NIOSH) with input from NCI, NIH, OSHA, CPSC, EPA, and ATSDR. The NTP has the capability to completely characterize the toxicologic profile of a chemical, including studies of chemical disposition, genetic toxicity, immunotoxicity, teratology, reproductive toxicity, carcinogenicity, neurotoxicity, and specific organ toxicity. The NTP encourages nominations of chemicals of human health concern from all sectors of the public, including industry, labor, and the general public. The specific process of nomination, evaluation, and selection of chemicals for testing by the NTP is described. It is a multicomponent system with several evaluations and a public peer review step to assure adequate consideration of all nominated chemicals. The results of NTP studies are all peer reviewed and available to the general public as well as to the scientific community. JF - Reproductive toxicology (Elmsford, N.Y.) AU - Heindel, J J AD - National Institute of Environmental Health Sciences, National Toxicology Program, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 273 EP - 279 VL - 2 IS - 3-4 SN - 0890-6238, 0890-6238 KW - Hazardous Substances KW - 0 KW - Index Medicus KW - United States KW - Animals KW - Reproduction -- drug effects KW - Hazardous Substances -- toxicity KW - Government Agencies -- organization & administration KW - Toxicology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78821065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.atitle=The+National+Toxicology+Program+chemical+nomination+selection+and+testing+process.&rft.au=Heindel%2C+J+J&rft.aulast=Heindel&rft.aufirst=J&rft.date=1988-01-01&rft.volume=2&rft.issue=3-4&rft.spage=273&rft.isbn=&rft.btitle=&rft.title=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.issn=08906238&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1992-05-07 N1 - Date created - 1992-05-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The relationship between ethanol intake and DSM-III alcohol use disorders: a cross-perspective analysis. AN - 78817942; 2980873 AB - The purpose of this investigation was to quantify the relationship between ethanol consumption and DSM-III alcohol use disorders using mathematical modeling techniques that allowed for the simultaneous control of several extraneous factors and the assessment of potential interaction. Although gender, education, ethnicity, and marital status were not identified as actual confounders in the logistic regression model, the ethanol intake-dependence association was found to be stronger among younger adults than in the later stages of life. Age was an influential confounder of the ethanol intake-abuse relationship, but the magnitude of the association was generally weaker for abuse than for dependence. Separate analyses in which the abuse and dependence criteria served as outcome measures qualified the interpretation of the overall ethanol intake-disorder associations. Implications of these results are discussed in terms of age differences in exposure and context of consumption, differential interpretation of withdrawal symptoms, and the relationship between abuse and dependence. The need for future research to refine our descriptions of risk of alcohol use disorders in relation to levels of intake is highlighted. JF - Journal of substance abuse AU - Grant, B F AU - Harford, T C AD - National Institute on Alcohol Abuse and Alcoholism, Division of Biometry and Epidemiology, Rockville, MD 20857. PY - 1988 SP - 231 EP - 252 VL - 1 IS - 2 SN - 0899-3289, 0899-3289 KW - Index Medicus KW - Age Factors KW - Risk Factors KW - Humans KW - Adult KW - Middle Aged KW - Adolescent KW - Psychometrics KW - Male KW - Female KW - Alcoholism -- diagnosis KW - Alcoholism -- classification KW - Alcohol Drinking -- psychology KW - Psychiatric Status Rating Scales -- statistics & numerical data KW - Alcoholism -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78817942?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+substance+abuse&rft.atitle=The+relationship+between+ethanol+intake+and+DSM-III+alcohol+use+disorders%3A+a+cross-perspective+analysis.&rft.au=Grant%2C+B+F%3BHarford%2C+T+C&rft.aulast=Grant&rft.aufirst=B&rft.date=1988-01-01&rft.volume=1&rft.issue=2&rft.spage=231&rft.isbn=&rft.btitle=&rft.title=Journal+of+substance+abuse&rft.issn=08993289&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1992-09-23 N1 - Date created - 1992-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The nephropathy of systemic lupus erythematosus. AN - 78807918; 2979809 AB - Lupus nephritis may be considered the prototypic autoimmune disease which is initiated by immune complex deposition. Several patterns of localization of immunoreactants are observed in lupus nephritis, but few data are available to elucidate the corresponding immunopathogenetic mechanisms. Corticosteroids form the mainstay of therapy for lupus nephritis, provided that they achieve a prompt clinical remission which can be sustained with alternate day therapy. Cyclophosphamide may have fewer risks than extended high-dose prednisone and it has been shown to be more efficacious than corticosteroids in preventing end stage renal failure. JF - In vivo (Athens, Greece) AU - Balow, J E AD - Kidney Disease Section, National Institutes of Health, Bethesda, Maryland 20892. PY - 1988 SP - 111 EP - 114 VL - 2 IS - 1 SN - 0258-851X, 0258-851X KW - Immunosuppressive Agents KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Azathioprine KW - MRK240IY2L KW - Index Medicus KW - Cyclophosphamide -- therapeutic use KW - Humans KW - Azathioprine -- therapeutic use KW - Lupus Nephritis -- drug therapy KW - Lupus Nephritis -- immunology KW - Immunosuppressive Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78807918?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=In+vivo+%28Athens%2C+Greece%29&rft.atitle=The+nephropathy+of+systemic+lupus+erythematosus.&rft.au=Balow%2C+J+E&rft.aulast=Balow&rft.aufirst=J&rft.date=1988-01-01&rft.volume=2&rft.issue=1&rft.spage=111&rft.isbn=&rft.btitle=&rft.title=In+vivo+%28Athens%2C+Greece%29&rft.issn=0258851X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1992-03-09 N1 - Date created - 1992-03-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Using time-to-pregnancy data to study occupational exposures: methodology. AN - 78807052; 2980347 AB - Occupational and environmental influences on fertility are for the most part unstudied, partly because sensitive methods for studying them have not been developed. We are developing a measure of fecundability, the monthly probability of pregnancy, by studying time to pregnancy, the number of noncontracepting menstrual cycles each couple requires to conceive. The relationship of this measure of reproductive impairment with others, such as spontaneous abortion, is not known. Preliminary data from two sources suggest that reduced fertility is not highly correlated with increased risk of spontaneous abortion, despite predictions to the contrary from the toxicology literature. A current study of occupational exposures of dental assistants will address questions of data quality by providing comparisons of brief responses from mail questionnaires and telephone interviews with very detailed data from telephone interviews. This study also will allow estimation of the magnitude of two potential selection biases: selection of only planned pregnancies (time-to-pregnancy data for accidental pregnancies are not meaningful), and selection against highly infertile and sterile couples (when studying currently or previously pregnant women, sterile couples are not represented at all and highly infertile couples are underrepresented). JF - Reproductive toxicology (Elmsford, N.Y.) AU - Baird, D D AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 205 EP - 207 VL - 2 IS - 3-4 SN - 0890-6238, 0890-6238 KW - Index Medicus KW - Humans KW - Time Factors KW - Female KW - Occupational Exposure KW - Pregnancy -- statistics & numerical data KW - Fertility -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78807052?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.atitle=Using+time-to-pregnancy+data+to+study+occupational+exposures%3A+methodology.&rft.au=Baird%2C+D+D&rft.aulast=Baird&rft.aufirst=D&rft.date=1988-01-01&rft.volume=2&rft.issue=3-4&rft.spage=205&rft.isbn=&rft.btitle=&rft.title=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.issn=08906238&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1992-05-07 N1 - Date created - 1992-05-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Teratogenicity of benzoic acid derivatives of retinoic acid in cultured mouse embryos. AN - 78806842; 2980411 AB - Isotretinoin (13-cis-RA) is a human teratogen and mouse embryos exposed to 13-cis-RA in vivo exhibit many of the same defects as humans. Early postimplantation mouse embryos exposed to 13-cis-RA in culture exhibit developmental alterations of the visceral arches, similar to those seen after in vivo exposure. Certain benzoic acid derivatives of retinoic acid have been shown to possess activity equal to or greater than retinoic acid in several in vitro systems. This study examines the teratogenic effects of some of these retinoids on mouse embryos in vitro. Day 8 CD-1 mouse embryos were cultured for 48 hours in the presence of these benzoic acid derivatives. With the exception of Ro-15-0778, all compounds produced visceral arch malformations similar to those seen in embryos exposed to 13-cis-RA, but at dramatically different effective concentrations. Extremely low concentrations of the retinoic acid-related compounds tested appear to have detrimental effects on embryonic development and these compounds may be poor candidates for therapeutic use. JF - Reproductive toxicology (Elmsford, N.Y.) AU - Goulding, E H AU - Jetten, A M AU - Abbott, B D AU - Pratt, R M AD - Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 91 EP - 98 VL - 2 IS - 2 SN - 0890-6238, 0890-6238 KW - Benzoates KW - 0 KW - Teratogens KW - Tretinoin KW - 5688UTC01R KW - Index Medicus KW - Molecular Structure KW - Animals KW - Culture Techniques KW - Mice -- embryology KW - Male KW - Female KW - Structure-Activity Relationship KW - Tretinoin -- analogs & derivatives KW - Tretinoin -- toxicity KW - Teratogens -- toxicity KW - Embryo, Mammalian -- drug effects KW - Benzoates -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78806842?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.atitle=Teratogenicity+of+benzoic+acid+derivatives+of+retinoic+acid+in+cultured+mouse+embryos.&rft.au=Goulding%2C+E+H%3BJetten%2C+A+M%3BAbbott%2C+B+D%3BPratt%2C+R+M&rft.aulast=Goulding&rft.aufirst=E&rft.date=1988-01-01&rft.volume=2&rft.issue=2&rft.spage=91&rft.isbn=&rft.btitle=&rft.title=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.issn=08906238&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1992-05-12 N1 - Date created - 1992-05-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Immunologic effects of drugs of abuse. AN - 78803177; 3151938 JF - NIDA research monograph AU - Weber, R AD - Section on Drug Design and Synthesis, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 99 EP - 104 VL - 90 SN - 1046-9516, 1046-9516 KW - Immunosuppressive Agents KW - 0 KW - Narcotics KW - Street Drugs KW - Index Medicus KW - Animals KW - Humans KW - Narcotics -- pharmacology KW - Immune System -- drug effects KW - Street Drugs -- pharmacology KW - Substance-Related Disorders -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78803177?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Immunologic+effects+of+drugs+of+abuse.&rft.au=Weber%2C+R&rft.aulast=Weber&rft.aufirst=R&rft.date=1988-01-01&rft.volume=90&rft.issue=&rft.spage=99&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-12-05 N1 - Date created - 1989-12-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - AIDS and intravenous drug abuse. AN - 78802339; 3151917 JF - NIDA research monograph AU - Schuster, C AU - Pickens, R AD - National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 13 VL - 90 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - AIDS/HIV KW - United States Public Health Service KW - Humans KW - Adult KW - Child KW - Adolescent KW - United States -- epidemiology KW - Male KW - Female KW - Acquired Immunodeficiency Syndrome -- epidemiology KW - Acquired Immunodeficiency Syndrome -- transmission KW - Substance Abuse, Intravenous -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78802339?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=AIDS+and+intravenous+drug+abuse.&rft.au=Schuster%2C+C%3BPickens%2C+R&rft.aulast=Schuster&rft.aufirst=C&rft.date=1988-01-01&rft.volume=90&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-12-05 N1 - Date created - 1989-12-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - TGF alpha stimulates growth of skin papillomas by autocrine and paracrine mechanisms but does not cause neoplastic progression. AN - 78790194; 2475136 AB - To investigate the role of transforming growth factor alpha (TGF alpha) in tumor development, we introduced the human TGF alpha (hTGF alpha) cDNA into cultured primary mouse epidermal cells or papilloma cells using a replication-defective retroviral vector and analyzed skin grafts constructed with such cells. Expression of the exogenous gene was confirmed by detection of hTGF alpha mRNA by northern RNA blot analysis, and the secreted hTGF alpha was measured by ELISA of culture supernatants. Tumor cells expressing hTGF alpha produced benign tumors (papillomas), which were 1.5- to 2-fold larger than tumors of parental cells when tested as skin grafts on nude mice. Grafts of normal cells that expressed hTGF alpha produced normal skin. When mixtures of parental tumor cells and normal mouse keratinocytes were grafted to nude mice, papilloma formation was suppressed and tumors that did form were small. Grafts of hTGF alpha-producing papilloma cells combined with either normal epidermal cells or hTGF alpha-producing epidermal cells yielded large tumors. Mixed grafts containing keratinocytes expressing hTGF alpha and parental papilloma cells also produced large tumors. While the tumor size was substantially increased by hTGF alpha expression, the tumors that developed in all groups were histologically benign and reached a stable size 4-5 wk after grafting. These results indicate that expression of hTGF alpha by either tumor cells (autocrine) or adjoining normal cells (paracrine) can stimulate tumor growth, particularly when tumor growth is suppressed by normal tissue. However, expression of this growth factor did not appear to influence tumor progression directly. JF - Molecular carcinogenesis AU - Finzi, E AU - Kilkenny, A AU - Strickland, J E AU - Balaschak, M AU - Bringman, T AU - Derynck, R AU - Aaronson, S AU - Yuspa, S H AD - Laboratories of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 7 EP - 12 VL - 1 IS - 1 SN - 0899-1987, 0899-1987 KW - RNA, Messenger KW - 0 KW - Keratins KW - 68238-35-7 KW - Transforming Growth Factors KW - 76057-06-2 KW - Index Medicus KW - Keratins -- genetics KW - Animals KW - Blotting, Northern KW - Skin -- metabolism KW - Disease Models, Animal KW - Mice KW - Mice, Nude KW - RNA, Messenger -- genetics KW - Keratins -- biosynthesis KW - Neoplasm Transplantation KW - Transfection KW - Blotting, Southern KW - Cell Line KW - Skin Neoplasms -- genetics KW - Papilloma -- pathology KW - Skin Neoplasms -- pathology KW - Papilloma -- genetics KW - Transforming Growth Factors -- physiology KW - Transforming Growth Factors -- biosynthesis KW - Transforming Growth Factors -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78790194?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=TGF+alpha+stimulates+growth+of+skin+papillomas+by+autocrine+and+paracrine+mechanisms+but+does+not+cause+neoplastic+progression.&rft.au=Finzi%2C+E%3BKilkenny%2C+A%3BStrickland%2C+J+E%3BBalaschak%2C+M%3BBringman%2C+T%3BDerynck%2C+R%3BAaronson%2C+S%3BYuspa%2C+S+H&rft.aulast=Finzi&rft.aufirst=E&rft.date=1988-01-01&rft.volume=1&rft.issue=1&rft.spage=7&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-09-28 N1 - Date created - 1989-09-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Thymic T-cell lymphoma with the CD8+ (OX-8), CD4+ (W3/25) phenotype, induced in F344/NCr rats by nitroso-2-hydroxypropylurea. AN - 78787624; 3267160 AB - Thymic T-cell lymphomas were induced by administration of nitroso-2-hydroxypropylurea (NHPU) at a dose of 1.6 mg by gavage twice weekly for each of 20 weeks in F344/NCr rats. Lymphomas began to appear 5-10 weeks after the first intubation. Primary and transplantable tumors were subjected to morphological, histogenetic and immunological characterization. Lymphomas composed of medium-sized lymphocytes (prolymphocytes) arose within individual thymic lobules, often in atrophic thymic lobes and metastasized slowly to the T-cell zones of the splenic white pulp. The tissue fixative was important for tumor morphology and classification, with Bouin's fluid markedly superior to formalin. Transplantable tumors were similar morphologically except for one lymphoblastic lymphoma, but all transplants metastasized quickly to spleen, liver, thymus, and other tissues. Fluorescence-activated cell sorter (FACS) analysis of primary and transplantable lymphomas revealed cells of the T-cell lineage, with tumor cells expressing both OX-8 (CD8) and W3/25 (CD4) antigens. Immunoperoxidase studies of spleen showed infiltration of OX-8+ tumor cells first into T-cell dependent areas of the splenic white pulp. JF - Thymus AU - Konishi, N AU - Ward, J M AU - Reynolds, C W AU - Lijinsky, W AD - Tumor Pathology and Pathogenesis Section, Division of Cancer Etiology, National Cancer Institute, Frederick, MD 21701. PY - 1988 SP - 225 EP - 237 VL - 12 IS - 4 SN - 0165-6090, 0165-6090 KW - Nitroso Compounds KW - 0 KW - Index Medicus KW - Rats KW - Phenotype KW - Neoplasm Transplantation KW - Animals KW - Rats, Inbred F344 KW - Male KW - Lymphoma -- genetics KW - Thymus Neoplasms -- genetics KW - Thymus Neoplasms -- chemically induced KW - Lymphoma -- chemically induced KW - Nitroso Compounds -- toxicity KW - Lymphoma -- pathology KW - Thymus Neoplasms -- pathology KW - T-Lymphocytes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78787624?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Thymus&rft.atitle=Thymic+T-cell+lymphoma+with+the+CD8%2B+%28OX-8%29%2C+CD4%2B+%28W3%2F25%29+phenotype%2C+induced+in+F344%2FNCr+rats+by+nitroso-2-hydroxypropylurea.&rft.au=Konishi%2C+N%3BWard%2C+J+M%3BReynolds%2C+C+W%3BLijinsky%2C+W&rft.aulast=Konishi&rft.aufirst=N&rft.date=1988-01-01&rft.volume=12&rft.issue=4&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Thymus&rft.issn=01656090&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-10-12 N1 - Date created - 1989-10-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Comparison of P+-active and -inactive pro-1 homologues from human nasopharyngeal carcinoma cells. AN - 78785641; 3255389 AB - Eleven pro-1 homologous clones from human nasopharyngeal carcinoma cell line CNE2 were studied with respect to their ability to transfer sensitivity to tumor promoter-induced neoplastic transformation (P+ activity), their molecular structure, and their homology to both mouse pro-1 and each other. Restriction mapping and Southern analysis of CNE2 pro-1 homologous clones revealed three structural classes, all of which showed P+ activity. The approximate limits of mouse pro-1 hybridizing sequences within CNE2 clones from each structural class were identified, and some of these minimum homologous sequences were tested for P+ activity by transfection into P- cells. For all classes, a strong association between P+ activity and pro-1 homology was observed. This finding implies that, for CNE2 clones to be P+-active, structural homology to mouse pro-1 is required. The minimum P+-active sequence thus far identified was a 2.6-kbp EcoRI-SstI fragment. One clone was inactive, even though it was indistinguishable from active clones of the same class by restriction mapping, Southern analysis, and electron microscope examination of heteroduplexes formed by an active and an inactive clone. This raises the possibility that discrete changes, involving a few nucleotides rather than gross rearrangement, may determine the P+ activation of these pro-1-homologous sequences. JF - Molecular carcinogenesis AU - Dowjat, W K AU - Ya, C AU - Nagashima, K AU - Sakai, A AU - Colburn, N H AD - Cell Biology Section, Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 33 EP - 40 VL - 1 IS - 1 SN - 0899-1987, 0899-1987 KW - DNA, Neoplasm KW - 0 KW - Nucleic Acid Heteroduplexes KW - Index Medicus KW - DNA, Neoplasm -- ultrastructure KW - Clone Cells KW - Sequence Homology, Nucleic Acid KW - Blotting, Southern KW - Nucleic Acid Heteroduplexes -- ultrastructure KW - Humans KW - Restriction Mapping KW - DNA, Neoplasm -- genetics KW - Mutation KW - Cell Line KW - Structure-Activity Relationship KW - Cloning, Molecular KW - Gene Expression Regulation KW - Nasopharyngeal Neoplasms -- genetics KW - Cell Transformation, Neoplastic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78785641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Comparison+of+P%2B-active+and+-inactive+pro-1+homologues+from+human+nasopharyngeal+carcinoma+cells.&rft.au=Dowjat%2C+W+K%3BYa%2C+C%3BNagashima%2C+K%3BSakai%2C+A%3BColburn%2C+N+H&rft.aulast=Dowjat&rft.aufirst=W&rft.date=1988-01-01&rft.volume=1&rft.issue=1&rft.spage=33&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-09-28 N1 - Date created - 1989-09-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Malignant conversion of murine squamous papilloma cell lines by transfection with the fos oncogene. AN - 78784545; 2475137 AB - Murine papilloma cell lines 308 and SP-1 have been used as recipients for transfected oncogenes to investigate malignant conversion. These cell lines express an activated c-rasHa gene with a codon 61 mutation and produce squamous papillomas when transplanted as skin grafts onto nude mice. They are not tumorigenic by subcutaneous injection. Both papilloma cell lines were stably transfected with plasmid DNA containing either a rearranged murine plasmacytoma-derived c-myc (minus exon 1), adenovirus 5 E1A, FBJ v-fos or a human c-fos/FBJ v-fos chimera, using cotransfection with the neomycin resistance gene contained in pSV2neo to select for transformants. Southern and northern blotting analysis confirmed the uptake and expression of exogenous DNA in both G418-selected cell lines and in the derived tumors. Unlike the E1A- and myc-containing plasmids, both fos constructs caused malignant conversion in either cell line, as defined by the squamous cell carcinoma histology of tumors from grafted cells and the development of carcinomas after subcutaneous injection into athymic nude mice. Immunofluorescence analysis for specific keratin gene expression indicated that tumors derived by introduction of either of the fos oncogenes were devoid of staining for K1, a 67 kDa epidermal keratin that is expressed in papillomas but not in squamous carcinomas. Tumors from E1A, myc, or pSV2neo transfectants expressed K1, although in a focal distribution. The malignant phenotype induced by the fos oncogene constructs was not associated with the ability to form agar colonies in vitro or to express gamma-glutamyl transpeptidase in the tumors. Since both 308 and SP-1 were sensitive to the fos oncogene for malignant conversion and insensitive to E1A or myc, it is possible that fos may cooperate with the endogenous-activated c-rasHa gene to convert these cells to malignancy. However, since gamma-glutamyl transpeptidase activity is found in the majority of chemically induced mouse skin carcinomas that possess an activated c-rasHa gene, fos activation may not be a common pathway for spontaneous malignant conversion. JF - Molecular carcinogenesis AU - Greenhalgh, D A AU - Yuspa, S H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 134 EP - 143 VL - 1 IS - 2 SN - 0899-1987, 0899-1987 KW - Biomarkers, Tumor KW - 0 KW - Proto-Oncogene Proteins KW - Proto-Oncogene Proteins c-fos KW - Keratins KW - 68238-35-7 KW - gamma-Glutamyltransferase KW - EC 2.3.2.2 KW - Proto-Oncogene Proteins p21(ras) KW - EC 3.6.5.2 KW - Index Medicus KW - Animals KW - Biomarkers, Tumor -- genetics KW - Immunoblotting KW - Papilloma -- pathology KW - gamma-Glutamyltransferase -- metabolism KW - Mice KW - Plasmids KW - Mice, Inbred BALB C KW - Papilloma -- genetics KW - Keratins -- biosynthesis KW - Neoplasm Transplantation KW - Proto-Oncogene Proteins -- genetics KW - Fluorescent Antibody Technique KW - Cell Line KW - Female KW - Cell Division KW - Oncogenes KW - Transfection KW - Cell Transformation, Neoplastic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78784545?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Malignant+conversion+of+murine+squamous+papilloma+cell+lines+by+transfection+with+the+fos+oncogene.&rft.au=Greenhalgh%2C+D+A%3BYuspa%2C+S+H&rft.aulast=Greenhalgh&rft.aufirst=D&rft.date=1988-01-01&rft.volume=1&rft.issue=2&rft.spage=134&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-09-29 N1 - Date created - 1989-09-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - raf family serine/threonine protein kinases in mitogen signal transduction. AN - 78779121; 2978288 JF - Cold Spring Harbor symposia on quantitative biology AU - Rapp, U R AU - Heidecker, G AU - Huleihel, M AU - Cleveland, J L AU - Choi, W C AU - Pawson, T AU - Ihle, J N AU - Anderson, W B AD - Laboratory of Viral Carcinogenesis, National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 173 EP - 184 VL - 53 Pt 1 SN - 0091-7451, 0091-7451 KW - Platelet-Derived Growth Factor KW - 0 KW - Proto-Oncogene Proteins KW - Protein Kinases KW - EC 2.7.- KW - Protein-Serine-Threonine Kinases KW - EC 2.7.11.1 KW - Proto-Oncogene Proteins c-raf KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Cells, Cultured KW - Humans KW - Genetic Vectors KW - Molecular Sequence Data KW - Mice KW - Amino Acid Sequence KW - Oncogenes -- drug effects KW - Signal Transduction -- drug effects KW - Platelet-Derived Growth Factor -- pharmacology KW - Protein Kinases -- genetics KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Gene Expression Regulation -- drug effects KW - Proto-Oncogene Proteins -- genetics KW - Proto-Oncogenes -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78779121?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cold+Spring+Harbor+symposia+on+quantitative+biology&rft.atitle=raf+family+serine%2Fthreonine+protein+kinases+in+mitogen+signal+transduction.&rft.au=Rapp%2C+U+R%3BHeidecker%2C+G%3BHuleihel%2C+M%3BCleveland%2C+J+L%3BChoi%2C+W+C%3BPawson%2C+T%3BIhle%2C+J+N%3BAnderson%2C+W+B&rft.aulast=Rapp&rft.aufirst=U&rft.date=1988-01-01&rft.volume=53+Pt+1&rft.issue=&rft.spage=173&rft.isbn=&rft.btitle=&rft.title=Cold+Spring+Harbor+symposia+on+quantitative+biology&rft.issn=00917451&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-09-15 N1 - Date created - 1989-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Signal transduction for proliferation and differentiation in keratinocytes. AN - 78764011; 2470295 AB - In mouse and human epidermis, the Ca2+ environment of the basal cell layer is substantially below serum Ca2+, while that of the granular cell layer is unusually high. Reduction of extracellular Ca2+ concentration (Cao) in the medium of keratinocyte cultures maintains a basal cell phenotype while serum Ca2+ concentrations induce terminal differentiation. Measurements of intracellular Ca2+ (Cai) by the use of Fura 2 and digital imaging technology reveal that Cai increases 10-20-fold in response to an increase in Cao and remains elevated. Concomitant with the rise in Cai is an increase in the metabolism of phosphatidylinositol (PI) to yield inositol phosphates and diacylglycerol. PI metabolism is also stimulated by calcium ionophores suggesting that a rise in Cai is directly responsible. The consequent increase in diacylglycerol and Cai would activate protein kinase C, an event known to trigger epidermal differentiation. Specific Cao and Cai determine the expression of individual markers of keratinocyte differentiation in vitro. These findings may account for the importance of the Ca2+ gradient for maintaining regulated growth and differentiation of the epidermis in vivo. JF - Annals of the New York Academy of Sciences AU - Yuspa, S H AU - Hennings, H AU - Tucker, R W AU - Jaken, S AU - Kilkenny, A E AU - Roop, D R AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 191 EP - 196 VL - 548 SN - 0077-8923, 0077-8923 KW - Antigens, Differentiation KW - 0 KW - Keratins KW - 68238-35-7 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Cells, Cultured KW - Humans KW - Antigens, Differentiation -- immunology KW - Calcium -- pharmacology KW - Cell Differentiation -- drug effects KW - Cell Division KW - Epidermis -- immunology KW - Epidermis -- cytology KW - Signal Transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78764011?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Signal+transduction+for+proliferation+and+differentiation+in+keratinocytes.&rft.au=Yuspa%2C+S+H%3BHennings%2C+H%3BTucker%2C+R+W%3BJaken%2C+S%3BKilkenny%2C+A+E%3BRoop%2C+D+R&rft.aulast=Yuspa&rft.aufirst=S&rft.date=1988-01-01&rft.volume=548&rft.issue=&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-19 N1 - Date created - 1989-06-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Characterization of activated proto-oncogenes in chemically transformed Syrian hamster embryo cells. AN - 78760195; 3074813 AB - The Syrian hamster embryo (SHE) cell transformation model has been used by many investigators to study the multistep process of neoplastic transformation induced by chemical carcinogens. In this study we have attempted to determine if activated proto-oncogenes are present in the transformed cells induced by a variety of chemical carcinogens. Twelve carcinogen-induced hamster cell lines, established by treatment of normal SHE cells with benzo[a]pyrene, diethylstilbestrol, or asbestos, were examined. One spontaneously transformed cell line (BHK-A) was also studied. Some of the cell lines were also tested for oncogene activation at the preneoplastic stage, before they acquired tumorigenic potential. DNAs from normal, preneoplastic, and neoplastic cells were tested by transfection into mouse NIH 3T3 cells, and morphologically transformed foci were scored on the contact-inhibited monolayer of 3T3 cells. The frequency of focus formation for normal SHE cell DNA was less than 0.0008 foci/microgram DNA, while approximately 40% (5 of 12) of the DNAs from carcinogen-induced, tumorigenic hamster cell lines induced foci at a frequency of greater than or equal to 0.012 foci/microgram DNA. The other seven carcinogen-induced cell lines and the BHK-A cells were negative (less than 0.002 foci/microgram DNA). When the DNAs from transformed foci induced by the five positive cell lines were retransfected into NIH 3T3 cells, the frequency of secondary foci of 3T3 cells was as much as 50-fold higher (1.34 foci/microgram DNA) than with the primary transfectants. DNAs from transformed foci or tumors derived from transformed foci were screened by Southern blot analyses with known oncogenes and with a hamster repetitive DNA probe for the presence of transfected hamster oncogenes.(ABSTRACT TRUNCATED AT 250 WORDS) JF - Molecular carcinogenesis AU - Gilmer, T M AU - Annab, L A AU - Barrett, J C AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 180 EP - 188 VL - 1 IS - 3 SN - 0899-1987, 0899-1987 KW - Asbestos KW - 1332-21-4 KW - Benzo(a)pyrene KW - 3417WMA06D KW - Diethylstilbestrol KW - 731DCA35BT KW - Index Medicus KW - Genes, ras KW - Animals KW - Oncogenes KW - Transfection KW - Embryo, Mammalian -- cytology KW - Mesocricetus KW - Cell Line, Transformed KW - Cricetinae KW - Cell Transformation, Neoplastic -- chemically induced KW - Gene Expression Regulation KW - Proto-Oncogenes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78760195?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Characterization+of+activated+proto-oncogenes+in+chemically+transformed+Syrian+hamster+embryo+cells.&rft.au=Gilmer%2C+T+M%3BAnnab%2C+L+A%3BBarrett%2C+J+C&rft.aulast=Gilmer&rft.aufirst=T&rft.date=1988-01-01&rft.volume=1&rft.issue=3&rft.spage=180&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-07-27 N1 - Date created - 1989-07-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus-associated oncogenes. AN - 78759494; 3074814 AB - Tumors produced by a chemically transformed rat liver epithelial (RLE) cell line and its single cell-derived clonal subpopulations demonstrate wide-ranging morphological presentations including carcinomas, sarcomas, "mixed epithelial-mesenchymal" tumors, and undifferentiated tumors [Am J Pathol 127:168-181, 1987]. To address the question of heterogeneity of tumors derived from transformed RLE cells, we have used recombinant retroviruses containing the following transforming oncogenes: v-raf (3611-MSV), v-raflv-myc (J2), v-myc (J5), and v-Ha-ras (pRNR16). All of the oncogenes, with the exception of v-myc (J5), were efficient transforming agents in the RLE cells. Tumors derived from the v-raf- and, to a lesser extent, those from v-Ha-ras-transformed RLE cells showed mixed epithelial-mesenchymal morphology, whereas the combination of v-raflv-myc (J2) consistently produced differentiated trabecular carcinomas. These data suggest that the lineage commitment of the RLE cells can be perturbed by a single transforming oncogene and that different tumor types derived from these cells may reflect the expression of a selective oncogene or a combination of oncogenes. JF - Molecular carcinogenesis AU - Garfield, S AU - Huber, B E AU - Nagy, P AU - Cordingley, M G AU - Thorgeirsson, S S AD - Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 189 EP - 195 VL - 1 IS - 3 SN - 0899-1987, 0899-1987 KW - Index Medicus KW - Rats KW - Genes, ras KW - Animals KW - Liver -- cytology KW - Epithelial Cells KW - Tumor Cells, Cultured -- pathology KW - Gene Expression Regulation KW - Cell Transformation, Viral KW - Oncogenes KW - Genes, Switch KW - Cell Transformation, Neoplastic -- genetics KW - Genes, Regulator UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78759494?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Neoplastic+transformation+and+lineage+switching+of+rat+liver+epithelial+cells+by+retrovirus-associated+oncogenes.&rft.au=Garfield%2C+S%3BHuber%2C+B+E%3BNagy%2C+P%3BCordingley%2C+M+G%3BThorgeirsson%2C+S+S&rft.aulast=Garfield&rft.aufirst=S&rft.date=1988-01-01&rft.volume=1&rft.issue=3&rft.spage=189&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-07-27 N1 - Date created - 1989-07-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The treatment of metastatic breast cancer with 5-fluorouracil and leucovorin. AN - 78756682; 3266821 JF - Advances in experimental medicine and biology AU - Allegra, C J AU - Egan, G F AU - Drake, J C AU - Steinberg, S M AU - Swain, S M AD - Clinical Pharmacology Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 107 EP - 112 VL - 244 SN - 0065-2598, 0065-2598 KW - Thymidylate Synthase KW - EC 2.1.1.45 KW - Leucovorin KW - Q573I9DVLP KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Fluorouracil -- administration & dosage KW - Thymidylate Synthase -- antagonists & inhibitors KW - Drug Evaluation KW - Drug Administration Schedule KW - Leucovorin -- administration & dosage KW - Humans KW - Neoplasm Metastasis KW - Female KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- pathology KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78756682?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+experimental+medicine+and+biology&rft.atitle=The+treatment+of+metastatic+breast+cancer+with+5-fluorouracil+and+leucovorin.&rft.au=Allegra%2C+C+J%3BEgan%2C+G+F%3BDrake%2C+J+C%3BSteinberg%2C+S+M%3BSwain%2C+S+M&rft.aulast=Allegra&rft.aufirst=C&rft.date=1988-01-01&rft.volume=244&rft.issue=&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Advances+in+experimental+medicine+and+biology&rft.issn=00652598&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-14 N1 - Date created - 1989-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Molecular cloning of mouse epidermal cystatin A and detection of regulated expression in differentiation and tumorigenesis. AN - 78753150; 2471537 AB - A gamma gt 11 cDNA expression library representing mouse epidermis mRNA was screened with polyclonal rabbit antiserum directed against 10-13 kDa epidermal antigens that had previously been shown to be regulated during epidermal differentiation. A cDNA clone was detected and isolated and its identity as the coding sequence for one of the antigens was confirmed by translation of hybrid-selected mRNA from mouse epidermis. The cDNA sequence predicted a peptide homologous to the reported sequence of rat epidermis cystatin A, a thiol proteinase inhibitor. This identification was confirmed by cross-reactivity of the gamma clone fusion protein with authentic antiserum to rat epidermis cystatin A. Southern gel analysis showed that mouse DNA may contain several closely related genes homologous to the cystatin probe. An mRNA of about 0.6 kb from epidermis and cultured mouse epidermal cells hybridized with the cystatin probe on northern analysis. The abundance of the message was high in cultured basal cells and was selectively diminished by inducing terminal differentiation in culture with an elevated Ca2+ concentration in the medium. Cystatin message was abundant in chemically induced mouse skin papillomas but reduced in carcinomas. In epidermis, mRNA was localized to the less differentiated basal and lower spinous layers by in situ hybridization. Regulation of expression of cystatin A in epidermis and tumors suggests that it may be important in the control of normal keratinocyte proliferation and differentiation and in malignant conversion. JF - Molecular carcinogenesis AU - Hawley-Nelson, P AU - Roop, D R AU - Cheng, C K AU - Krieg, T M AU - Yuspa, S H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 202 EP - 211 VL - 1 IS - 3 SN - 0899-1987, 0899-1987 KW - Cysteine Proteinase Inhibitors KW - 0 KW - Protease Inhibitors KW - RNA, Messenger KW - Keratins KW - 68238-35-7 KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Keratins -- physiology KW - Base Sequence KW - Blotting, Southern KW - Epidermis -- cytology KW - Cell Differentiation KW - Mice KW - Gene Expression Regulation KW - Amino Acid Sequence KW - Nucleic Acid Hybridization KW - RNA, Messenger -- isolation & purification KW - Cross Reactions KW - Skin Neoplasms -- genetics KW - Protease Inhibitors -- genetics KW - Cloning, Molecular UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78753150?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Molecular+cloning+of+mouse+epidermal+cystatin+A+and+detection+of+regulated+expression+in+differentiation+and+tumorigenesis.&rft.au=Hawley-Nelson%2C+P%3BRoop%2C+D+R%3BCheng%2C+C+K%3BKrieg%2C+T+M%3BYuspa%2C+S+H&rft.aulast=Hawley-Nelson&rft.aufirst=P&rft.date=1988-01-01&rft.volume=1&rft.issue=3&rft.spage=202&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-07-27 N1 - Date created - 1989-07-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Implication of protein oxidation in protein turnover, aging, and oxygen toxicity. AN - 78752432; 3150666 AB - It is evident from the results summarized here that a variety of MFO systems catalyze the oxidation inactivation of enzymes. This likely involves site-directed Fenton-chemistry in which Fe(II) bound to metal binding sites on the protein undergoes peroxidation to form active oxygen species that convert proximal amino acid residues to carbonyl derivatives. Such oxidation is likely involved in the accumulation of altered enzymes during aging, in premature aging diseases, in the killing of bacteria by neutrophils and in protein turnover. In view of these results, the possibility that protein oxidation is implicated in various diseases, viz, arthritis, pulmonary dysfunction, and carcinogenesis deserves consideration. JF - Basic life sciences AU - Stadtman, E R AU - Oliver, C N AU - Levine, R L AU - Fucci, L AU - Rivett, A J AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 331 EP - 339 VL - 49 SN - 0090-5542, 0090-5542 KW - Enzymes KW - 0 KW - Peptides KW - Edetic Acid KW - 9G34HU7RV0 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Space life sciences KW - Animals KW - Enzyme Stability KW - Aging KW - Edetic Acid -- pharmacology KW - Oxygen -- toxicity KW - Oxygen -- pharmacology KW - Enzymes -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78752432?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Basic+life+sciences&rft.atitle=Implication+of+protein+oxidation+in+protein+turnover%2C+aging%2C+and+oxygen+toxicity.&rft.au=Stadtman%2C+E+R%3BOliver%2C+C+N%3BLevine%2C+R+L%3BFucci%2C+L%3BRivett%2C+A+J&rft.aulast=Stadtman&rft.aufirst=E&rft.date=1988-01-01&rft.volume=49&rft.issue=&rft.spage=331&rft.isbn=&rft.btitle=&rft.title=Basic+life+sciences&rft.issn=00905542&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-23 N1 - Date created - 1989-06-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neoplastic transformation of a human bronchial epithelial cell line by a recombinant retrovirus encoding viral Harvey ras. AN - 78751973; 2855021 AB - Activated ras oncogenes have previously been implicated in the pathogenesis of human lung carcinomas. A v-Ha-ras-containing retrovirus, Zip-ras, was generated by inserting the coding region of the v-Ha-ras oncogene into the Zip-NeoSV(X) [Cepko et al., Cell 37:1053-1062, 1984] retroviral vector. Amphotrophic Zip-ras retrovirus was used to infect an SV40 large T antigen-positive immortalized cell line, BEAS-2B, derived from normal bronchial epithelial cells, the predominant progenitor cells of human lung carcinomas. Zip-ras-infected BEAS-2B cells selected for G418 resistance formed anaplastic carcinomas in 12 of 15 athymic nude mice (latency 3 wk), whereas Zip-NeoSV(X)-infected BEAS-2B control cultures inoculated into 12 nude mice formed no tumors after a minimum of 7 mo. Tumor cell lines were established and demonstrated to be of human epithelial origin and to express v-Ha-ras p21 protein. A common feature of the tumor cell lines was an increase in ploidy. The increased efficiency of neoplastic transformation by v-Ha-ras of cell lines as compared with our previous results with normal bronchial epithelial cells [Yoakum et al., Science 227:1174-1179, 1985] is consistent with the hypothesis that the "immortalization" step is rate-limiting in in vitro human epithelial cell carcinogenesis. JF - Molecular carcinogenesis AU - Amstad, P AU - Reddel, R R AU - Pfeifer, A AU - Malan-Shibley, L AU - Mark, G E AU - Harris, C C AD - Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 151 EP - 160 VL - 1 IS - 3 SN - 0899-1987, 0899-1987 KW - Isoenzymes KW - 0 KW - Index Medicus KW - Animals KW - Humans KW - Simian virus 40 -- genetics KW - Mice KW - Mice, Nude KW - Bronchial Neoplasms -- pathology KW - Epithelial Cells KW - Recombination, Genetic KW - Carcinogenicity Tests KW - Tumor Cells, Cultured -- pathology KW - Cell Line KW - Cell Transformation, Viral KW - Genes, ras KW - Bronchi -- cytology KW - Cell Transformation, Neoplastic -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78751973?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+carcinogenesis&rft.atitle=Neoplastic+transformation+of+a+human+bronchial+epithelial+cell+line+by+a+recombinant+retrovirus+encoding+viral+Harvey+ras.&rft.au=Amstad%2C+P%3BReddel%2C+R+R%3BPfeifer%2C+A%3BMalan-Shibley%2C+L%3BMark%2C+G+E%3BHarris%2C+C+C&rft.aulast=Amstad&rft.aufirst=P&rft.date=1988-01-01&rft.volume=1&rft.issue=3&rft.spage=151&rft.isbn=&rft.btitle=&rft.title=Molecular+carcinogenesis&rft.issn=08991987&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-07-27 N1 - Date created - 1989-07-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Oxidative modification of enzymes during aging and acute oxidative stress. AN - 78751430; 2907971 JF - Basic life sciences AU - Starke-Reed, P E AU - Oliver, C N AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 537 EP - 540 VL - 49 SN - 0090-5542, 0090-5542 KW - Enzymes KW - 0 KW - Mixed Function Oxygenases KW - EC 1.- KW - Glucosephosphate Dehydrogenase KW - EC 1.1.1.49 KW - Glutamate-Ammonia Ligase KW - EC 6.3.1.2 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Rats KW - Oxidation-Reduction KW - Animals KW - Glutamate-Ammonia Ligase -- metabolism KW - Kinetics KW - Aging KW - Glucosephosphate Dehydrogenase -- metabolism KW - Liver -- enzymology KW - Mixed Function Oxygenases -- metabolism KW - Oxygen -- toxicity KW - Liver -- growth & development KW - Liver -- drug effects KW - Enzymes -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78751430?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Basic+life+sciences&rft.atitle=Oxidative+modification+of+enzymes+during+aging+and+acute+oxidative+stress.&rft.au=Starke-Reed%2C+P+E%3BOliver%2C+C+N&rft.aulast=Starke-Reed&rft.aufirst=P&rft.date=1988-01-01&rft.volume=49&rft.issue=&rft.spage=537&rft.isbn=&rft.btitle=&rft.title=Basic+life+sciences&rft.issn=00905542&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-23 N1 - Date created - 1989-06-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The medical and metabolic consequences of administration of sodium acetate. AN - 78750531; 2854950 AB - 1. The standard total parenteral nutrition, peritoneal dialysis, hemodialysis and many surgical fluids in use today contain 36 to 45 mM D,L-lactate or 2 to 140 mM acetate whereas the normal blood level of D-lactate is 0.02 mM L-lactate 0.5 to 5 mM and acetate 0.1 nM. The reasons for the continued use in patients of such unphysiological concentrations of these anions appear to be historic. 2. Administration of similar concentrations of these anions to the rat causes widespread metabolic disturbances which mimic many of the untoward complications associated with current parenteral and dialysis therapy. Understanding of the mechanisms attendant upon the metabolism of these anions may serve as a guide for designing improved parenteral fluids for human patients. 3. Elevation of blood D-lactate to 5 mM is associated with cerebral dysfunction in human patients. 4. Acetate stimulates the release of the inflammatory leukokine, interleukin-1 from human monocytes. Use of 35 to 45 mM acetate in peritoneal dialysis fluids led to peritoneal fibrosis. Patients exposed to acetate containing hemodialysis fluids have 12-fold elevation in their plasma interleukin-1 levels. 5. Administration of 20 mM sodium acetate to rats leads to a number of metabolic disturbances similar to those seen in human dialysis patients: (a) Acetate elevates blood glucose in the rat and may contribute to the exacerbation of the carbohydrate intolerance seen in uremic patients. (b) Acetate increases the levels of hepatic malonyl CoA, the rate controlling substrate of fatty acid synthesis and may exacerbate the hypertriglyceridemia characteristic of dialysis patients. (c) Acetate administration in the rat leads to a decrease in the cytosolic phosphorylation potential, reduction of the redox state of the free cytosolic NAD couple and paradoxical oxidation of the mitochondrial NAD couple in a pattern analogous to that produced by uncouplers of oxidative phosphorylation and may account in part for the elevation of temperature reported in patients undergoing hemodialysis with acetate. (d) Acetate administration in the rat leads to an increase in intracellular phosphorylated intermediates, adenine nucleotides, inorganic phosphate, inorganic pyrophosphate, calcium and magnesium. On cessation of acetate metabolism, the inorganic phosphate and calcium accumulated intracellularly leave the intracellular space. In patients undergoing hemodialysis, the blood phosphate returns to predialysis levels, within 6 hr after the completion of treatment, leaving significant numbers of patients with chronic hyperphosphatemia and the multiple complications attendant to that state.(ABSTRACT TRUNCATED AT 400 WORDS) JF - Advances in enzyme regulation AU - Veech, R L AU - Gitomer, W L AD - Laboratory of Metabolism and Molecular Biology, NIAAA, Rockville, Maryland 20852. Y1 - 1988 PY - 1988 DA - 1988 SP - 313 EP - 343 VL - 27 SN - 0065-2571, 0065-2571 KW - Acetates KW - 0 KW - Dialysis Solutions KW - Diphosphates KW - Magnesium Compounds KW - Phosphates KW - magnesium pyrophosphate KW - AL34Y660JV KW - Magnesium KW - I38ZP9992A KW - Glucose KW - IY9XDZ35W2 KW - Acetic Acid KW - Q40Q9N063P KW - Coenzyme A KW - SAA04E81UX KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Phosphates -- blood KW - Coenzyme A -- metabolism KW - Diphosphates -- metabolism KW - Glucose -- metabolism KW - Humans KW - Liver -- metabolism KW - Homeostasis -- drug effects KW - Rats, Inbred Strains KW - Rats KW - Oxidation-Reduction KW - Calcium -- metabolism KW - Magnesium -- metabolism KW - Mitochondria -- metabolism KW - Male KW - Dialysis Solutions -- adverse effects KW - Parenteral Nutrition -- adverse effects KW - Acetates -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78750531?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+enzyme+regulation&rft.atitle=The+medical+and+metabolic+consequences+of+administration+of+sodium+acetate.&rft.au=Veech%2C+R+L%3BGitomer%2C+W+L&rft.aulast=Veech&rft.aufirst=R&rft.date=1988-01-01&rft.volume=27&rft.issue=&rft.spage=313&rft.isbn=&rft.btitle=&rft.title=Advances+in+enzyme+regulation&rft.issn=00652571&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-07-03 N1 - Date created - 1989-07-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The National Cancer Institute experience with early and late effects of combined modality therapy in small cell lung cancer. AN - 78749742; 2854442 JF - Antibiotics and chemotherapy AU - Johnson, B E AU - Glatstein, E AU - Ihde, D C AD - NCI-Navy Medical Oncology Branch, Bethesda, Md. Y1 - 1988 PY - 1988 DA - 1988 SP - 210 EP - 212 VL - 41 SN - 0066-4758, 0066-4758 KW - Index Medicus KW - United States KW - Combined Modality Therapy KW - Humans KW - National Institutes of Health (U.S.) KW - Clinical Trials as Topic KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Time Factors KW - Radiation Injuries KW - Male KW - Female KW - Carcinoma, Small Cell -- therapy KW - Lung Neoplasms -- radiotherapy KW - Carcinoma, Small Cell -- mortality KW - Lung Neoplasms -- drug therapy KW - Carcinoma, Small Cell -- radiotherapy KW - Lung Neoplasms -- therapy KW - Lung Neoplasms -- mortality KW - Carcinoma, Small Cell -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78749742?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antibiotics+and+chemotherapy&rft.atitle=The+National+Cancer+Institute+experience+with+early+and+late+effects+of+combined+modality+therapy+in+small+cell+lung+cancer.&rft.au=Johnson%2C+B+E%3BGlatstein%2C+E%3BIhde%2C+D+C&rft.aulast=Johnson&rft.aufirst=B&rft.date=1988-01-01&rft.volume=41&rft.issue=&rft.spage=210&rft.isbn=&rft.btitle=&rft.title=Antibiotics+and+chemotherapy&rft.issn=00664758&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-26 N1 - Date created - 1989-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - A Ca2+-dependent signal transduction system participates in coupling expression of some cAMP-dependent prespore genes to the cell surface receptor. AN - 78748649; 2854023 AB - Elevated levels of cAMP are essential for the expression of many postaggregation prespore and prestalk mRNA species and for the suppression of some growth phase mRNAs. Here we review evidence that this regulation is mediated by cAMP interacting at the cell surface receptor. These effects of cAMP on gene expression can occur under conditions where the receptor-associated adenylate cyclase is inactivated and in concentrations that are consistent with receptor-binding. A number of differences are noted in the mechanism by which cAMP regulates prespore and prestalk genes. Finally, evidence is reviewed for the role of a Ca2+-dependent signal transduction system in coupling the expression of some of the prespore mRNAs to the cAMP receptor. This signal transduction system does not appear to be involved in the expression of the cAMP-dependent prestalk gene. JF - Developmental genetics AU - Blumberg, D D AU - Comer, J F AU - Higinbotham, K G AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick, Maryland. Y1 - 1988 PY - 1988 DA - 1988 SP - 359 EP - 369 VL - 9 IS - 4-5 SN - 0192-253X, 0192-253X KW - Receptors, Cyclic AMP KW - 0 KW - Cyclic AMP KW - E0399OZS9N KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Spores, Fungal -- physiology KW - Dictyostelium -- genetics KW - Receptors, Cyclic AMP -- physiology KW - Genes, Fungal KW - Calcium -- physiology KW - Dictyostelium -- physiology KW - Transcription, Genetic KW - Gene Expression Regulation KW - Cyclic AMP -- physiology KW - Signal Transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78748649?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+genetics&rft.atitle=A+Ca2%2B-dependent+signal+transduction+system+participates+in+coupling+expression+of+some+cAMP-dependent+prespore+genes+to+the+cell+surface+receptor.&rft.au=Blumberg%2C+D+D%3BComer%2C+J+F%3BHiginbotham%2C+K+G&rft.aulast=Blumberg&rft.aufirst=D&rft.date=1988-01-01&rft.volume=9&rft.issue=4-5&rft.spage=359&rft.isbn=&rft.btitle=&rft.title=Developmental+genetics&rft.issn=0192253X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-26 N1 - Date created - 1989-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Oxidative modification of enzymes by stimulated neutrophils. AN - 78744757; 2907973 JF - Basic life sciences AU - Oliver, C N AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 839 EP - 844 VL - 49 SN - 0090-5542, 0090-5542 KW - Glutamate-Ammonia Ligase KW - EC 6.3.1.2 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Oxidation-Reduction KW - Humans KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Cell Differentiation KW - Cell Line KW - Neutrophils -- drug effects KW - Glutamate-Ammonia Ligase -- antagonists & inhibitors KW - Neutrophils -- physiology KW - Escherichia coli -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78744757?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Basic+life+sciences&rft.atitle=Oxidative+modification+of+enzymes+by+stimulated+neutrophils.&rft.au=Oliver%2C+C+N&rft.aulast=Oliver&rft.aufirst=C&rft.date=1988-01-01&rft.volume=49&rft.issue=&rft.spage=839&rft.isbn=&rft.btitle=&rft.title=Basic+life+sciences&rft.issn=00905542&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-23 N1 - Date created - 1989-06-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Reduction of nitroheterocyclic drugs by ascorbate and catecholamines: a possible mechanism for the neurotoxicity of nitroheterocyclic drugs. AN - 78744697; 3250533 JF - Basic life sciences AU - Rao, D N AU - Mason, R P AD - Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 787 EP - 794 VL - 49 SN - 0090-5542, 0090-5542 KW - Catecholamines KW - 0 KW - Free Radicals KW - Neurotoxins KW - Metronidazole KW - 140QMO216E KW - Misonidazole KW - 8FE7LTN8XE KW - Nitrofurantoin KW - 927AH8112L KW - Catalase KW - EC 1.11.1.6 KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Ascorbic Acid KW - PQ6CK8PD0R KW - Index Medicus KW - Rats KW - Oxidation-Reduction KW - Catalase -- metabolism KW - Animals KW - Oxygen Consumption KW - Biotransformation KW - Kinetics KW - Superoxide Dismutase -- metabolism KW - Male KW - Nitrofurantoin -- metabolism KW - Catecholamines -- metabolism KW - Metronidazole -- metabolism KW - Liver -- metabolism KW - Misonidazole -- metabolism KW - Ascorbic Acid -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78744697?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Basic+life+sciences&rft.atitle=Reduction+of+nitroheterocyclic+drugs+by+ascorbate+and+catecholamines%3A+a+possible+mechanism+for+the+neurotoxicity+of+nitroheterocyclic+drugs.&rft.au=Rao%2C+D+N%3BMason%2C+R+P&rft.aulast=Rao&rft.aufirst=D&rft.date=1988-01-01&rft.volume=49&rft.issue=&rft.spage=787&rft.isbn=&rft.btitle=&rft.title=Basic+life+sciences&rft.issn=00905542&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-23 N1 - Date created - 1989-06-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Toxicity and carcinogenicity studies of phenylephrine hydrochloride in F344/N rats and B6C3F1 mice. AN - 78737559; 3243185 AB - Phenylephrine HCl was incorporated into feed given to male and female F344/N rats and B6C3F1 mice in studies of 14 days, 12 weeks, and 2 years duration. In 12-week studies, body weight gains decreased with dose, and deaths of male rats and mice occurred at concentrations of 5,000 ppm and above; however, no organ-specific toxicity was evident. During 2-year studies, body weights of rats receiving diets at 620 and 1,250 ppm and mice at 1,250 and 2,500 ppm ranged up to 16% less than control. Survival of high dose male rats was substantially greater than controls. Survivals of other dose groups of rats and mice were similar to controls. Chronic focal inflammation of the liver, and inflammation of the prostate were increased in dosed rats. No increases in neoplasms were observed in rats or mice consuming diets containing phenylephrine HCl for 2 years. The incidences of mononuclear cell leukemia and pheochromocytomas of the adrenal gland were decreased in dosed male rats. Approximate time weighted average doses ranged up to 54 mg/kg/day for rats and 280 mg/kg/day for mice during the 2-year studies. JF - Drug and chemical toxicology AU - Bucher, J R AU - Huff, J AU - Haseman, J K AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 355 EP - 370 VL - 11 IS - 4 SN - 0148-0545, 0148-0545 KW - Phenylephrine KW - 1WS297W6MV KW - Index Medicus KW - Rats KW - Prostate -- drug effects KW - Mice, Inbred Strains KW - Animals KW - Rats, Inbred F344 KW - Sex Factors KW - Liver -- drug effects KW - Body Weight -- drug effects KW - Mice KW - Hematopoietic System -- drug effects KW - Adrenal Glands -- drug effects KW - Male KW - Female KW - Phenylephrine -- toxicity KW - Carcinogenicity Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78737559?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+and+chemical+toxicology&rft.atitle=Toxicity+and+carcinogenicity+studies+of+phenylephrine+hydrochloride+in+F344%2FN+rats+and+B6C3F1+mice.&rft.au=Bucher%2C+J+R%3BHuff%2C+J%3BHaseman%2C+J+K&rft.aulast=Bucher&rft.aufirst=J&rft.date=1988-01-01&rft.volume=11&rft.issue=4&rft.spage=355&rft.isbn=&rft.btitle=&rft.title=Drug+and+chemical+toxicology&rft.issn=01480545&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-26 N1 - Date created - 1989-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alcohol intoxication reduces visual sustained attention. AN - 78729092; 3149763 AB - Effects of alcohol intoxication on visual sustained attention were studied using a vigilance task entailing detection of degraded target stimuli. Data were obtained in separate sessions under four ethanol doses, ranging from 0 (placebo) to 1.05 g/kg lean body weight, with periodic maintenance dosing of 0.12 g/kg. Intoxication lowered the overall level of detection performance, and in addition produced dose-related increases in the rate of performance decrement over time. Analysis of performance data using techniques derived from Signal Detection Theory indicated that the decrements were due specifically to alterations in perceptual sensitivity. Examination of eye movements and blinks indicated that the effects of ethanol were not mediated peripherally. Rather, alcohol appears to have deleterious effects on central processing capacity and the availability of capacity over time. The alcohol-related failure of sustained attention may contribute to increased accident risk in tasks requiring continuous performance. JF - Psychopharmacology AU - Rohrbaugh, J W AU - Stapleton, J M AU - Parasuraman, R AU - Frowein, H W AU - Adinoff, B AU - Varner, J L AU - Zubovic, E A AU - Lane, E A AU - Eckardt, M J AU - Linnoila, M AD - Laboratory of Clinical Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 442 EP - 446 VL - 96 IS - 4 SN - 0033-3158, 0033-3158 KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - Reaction Time -- drug effects KW - Eye Movements -- drug effects KW - Ethanol -- blood KW - Psychomotor Performance -- drug effects KW - Humans KW - Adult KW - Male KW - Alcoholic Intoxication -- psychology KW - Attention -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78729092?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Alcohol+intoxication+reduces+visual+sustained+attention.&rft.au=Rohrbaugh%2C+J+W%3BStapleton%2C+J+M%3BParasuraman%2C+R%3BFrowein%2C+H+W%3BAdinoff%2C+B%3BVarner%2C+J+L%3BZubovic%2C+E+A%3BLane%2C+E+A%3BEckardt%2C+M+J%3BLinnoila%2C+M&rft.aulast=Rohrbaugh&rft.aufirst=J&rft.date=1988-01-01&rft.volume=96&rft.issue=4&rft.spage=442&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-06-02 N1 - Date created - 1989-06-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Acceptance of HIV-antibody testing by persons seeking outpatient treatment for cocaine abuse. AN - 78718470; 3236388 AB - One hundred of 101 applicants for outpatient treatment for cocaine abuse consented to voluntary HIV-antibody testing when the testing was offered as an option within the medical assessment at intake. Twelve applicants tested HIV-antibody positive; eight of these had injected drugs parenterally with syringes and needles used by other addicts and four had never taken drugs intravenously. There were no significant differences between HIV-antibody positive and negative applicants regarding the percentages who completed the evaluation, began treatment, and completed four weeks of treatment. A subgroup of 48 patients were interviewed regarding their knowledge of HIV infection, AIDS, and risk factors associated with transmission of HIV. All 48 patients had heard of HIV, AIDS, and recommendations that they use condoms as well as clean syringes and needles. None of the 48 patients reported that they used condoms; 10 reported reduced sexual activity and number of sexual partners; and none of those who shared needles reported that they had discontinued sharing other addicts' drug paraphernalia. The authors conclude that on-site, voluntary HIV-antibody testing for drug abusing patients entering treatment appears feasible and is not a deterrent to persons entering and continuing in treatment for drug abuse. The finding that persons at risk for HIV infection have knowledge of risk factors and have not changed risk-taking behaviors associated with HIV contagion points out the urgent need for further education and counseling. JF - Journal of substance abuse treatment AU - Weddington, W W AU - Brown, B S AD - Addiction Research Center/National Institute on Drug Abuse, Baltimore, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 145 EP - 149 VL - 5 IS - 3 SN - 0740-5472, 0740-5472 KW - HIV Antibodies KW - 0 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - AIDS/HIV KW - Risk Factors KW - Humans KW - HIV Seropositivity -- diagnosis KW - Adult KW - Middle Aged KW - Ambulatory Care Facilities KW - Male KW - Female KW - Acquired Immunodeficiency Syndrome -- diagnosis KW - Substance-Related Disorders -- therapy KW - Patient Acceptance of Health Care KW - HIV Antibodies -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78718470?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+substance+abuse+treatment&rft.atitle=Acceptance+of+HIV-antibody+testing+by+persons+seeking+outpatient+treatment+for+cocaine+abuse.&rft.au=Weddington%2C+W+W%3BBrown%2C+B+S&rft.aulast=Weddington&rft.aufirst=W&rft.date=1988-01-01&rft.volume=5&rft.issue=3&rft.spage=145&rft.isbn=&rft.btitle=&rft.title=Journal+of+substance+abuse+treatment&rft.issn=07405472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-08 N1 - Date created - 1989-05-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Mammalian genome organization: an evolutionary view. AN - 78715849; 3071253 JF - Annual review of genetics AU - O'Brien, S J AU - Seuánez, H N AU - Womack, J E AD - Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland 21701-1013. Y1 - 1988 PY - 1988 DA - 1988 SP - 323 EP - 351 VL - 22 SN - 0066-4197, 0066-4197 KW - Index Medicus KW - Animals KW - Humans KW - Chromosomes, Human KW - Genes KW - Biological Evolution KW - Mammals -- genetics KW - Chromosome Mapping UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78715849?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annual+review+of+genetics&rft.atitle=Mammalian+genome+organization%3A+an+evolutionary+view.&rft.au=O%27Brien%2C+S+J%3BSeu%C3%A1nez%2C+H+N%3BWomack%2C+J+E&rft.aulast=O%27Brien&rft.aufirst=S&rft.date=1988-01-01&rft.volume=22&rft.issue=&rft.spage=323&rft.isbn=&rft.btitle=&rft.title=Annual+review+of+genetics&rft.issn=00664197&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-10 N1 - Date created - 1989-05-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Aberrations of growth and differentiation pathways during neoplastic transformation of human epithelial cells. AN - 78715646; 3069724 JF - IARC scientific publications AU - Harris, C C AU - Brash, D E AU - Lechner, J F AU - Mark, G AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 139 EP - 148 IS - 92 SN - 0300-5038, 0300-5038 KW - Index Medicus KW - Phenotype KW - Animals KW - Humans KW - Cell Differentiation KW - Gene Expression Regulation KW - Cell Transformation, Neoplastic -- pathology KW - Epithelium -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78715646?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=IARC+scientific+publications&rft.atitle=Aberrations+of+growth+and+differentiation+pathways+during+neoplastic+transformation+of+human+epithelial+cells.&rft.au=Harris%2C+C+C%3BBrash%2C+D+E%3BLechner%2C+J+F%3BMark%2C+G&rft.aulast=Harris&rft.aufirst=C&rft.date=1988-01-01&rft.volume=&rft.issue=92&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=IARC+scientific+publications&rft.issn=03005038&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-04 N1 - Date created - 1989-05-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Aberrant differentiation in mouse skin carcinogenesis. AN - 78713415; 2466787 JF - IARC scientific publications AU - Yuspa, S H AU - Kilkenny, A AU - Roop, D R AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 3 EP - 10 IS - 92 SN - 0300-5038, 0300-5038 KW - Keratins KW - 68238-35-7 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Keratins -- metabolism KW - Calcium -- metabolism KW - Animals KW - Cell Transformation, Neoplastic -- pathology KW - Neoplastic Stem Cells -- pathology KW - Cell Differentiation KW - Mice KW - Skin Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78713415?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=IARC+scientific+publications&rft.atitle=Aberrant+differentiation+in+mouse+skin+carcinogenesis.&rft.au=Yuspa%2C+S+H%3BKilkenny%2C+A%3BRoop%2C+D+R&rft.aulast=Yuspa&rft.aufirst=S&rft.date=1988-01-01&rft.volume=&rft.issue=92&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=IARC+scientific+publications&rft.issn=03005038&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-05-04 N1 - Date created - 1989-05-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Lung cancer chemoprevention with retinol palmitate. Preliminary data from a randomized trial on stage Ia non small-cell lung cancer. AN - 78699729; 2852945 AB - On the basis of epidemiologic and experimental evidence of an anticancer activity of vitamin A, a randomized clinical trial was activated in Milan with the aim of evaluating if retinol palmitate administration (per os, 300,000 I.U. daily) after complete resection of stage Ia non small-cell lung cancer could reduce the occurrence of cancer relapses (within 3 years) and/or the occurrence of new primary tumors (beyond 3 years). By September 1987, 181 patients had entered the trial: 87 in the treatment arm and 94 in the control arm. After a median follow-up of 14 months, the interim analysis was focused on the evaluation of toxicity, compliance, and early recurrences. Although the large majority of patients were affected by skin and mucous membrane desquamation and dryness during treatment, these symptoms were generally mild and well tolerated, and never induced the patient to stop the treatment. Other side effects like headache, hair loss, itching, or dyspepsia were detected at a much lower frequency. Only in 3 patients the treatment was interrupted, because of signs or symptoms potentially related to vitamin A administration. At the time of the analysis, a total of 42 (23%) patients had relapsed; 16 (18%) in the treated arm, and 26 (28%) in the control arm. The largest difference between treated patients and controls was observed for bone metastases (2 vs. 7) and brain metastases (3 vs. 6), and for squamous histology (6 vs. 11). Only 2 cases of new primary cancer were detected, both in the control arm. These results are promising both in terms of tolerance and efficacy of treatment, but given the short median follow-up they must be very cautiously interpreted. A longer follow-up is necessary to establish whether a significant proportion of early recurrences could be prevented, or only delayed, by vitamin A administration. JF - Acta oncologica (Stockholm, Sweden) AU - Pastorino, U AU - Soresi, E AU - Clerici, M AU - Chiesa, G AU - Belloni, P A AU - Ongari, M AU - Valente, M AU - Ravasi, G AD - Department of Chest Surgery, National Cancer Institute, Milan, Italy. Y1 - 1988 PY - 1988 DA - 1988 SP - 773 EP - 782 VL - 27 IS - 6b SN - 0284-186X, 0284-186X KW - Vitamin A KW - 11103-57-4 KW - Index Medicus KW - Infant KW - Neoplasm Staging KW - Random Allocation KW - Humans KW - Adult KW - Infant, Newborn KW - Clinical Trials as Topic KW - Aged KW - Middle Aged KW - Child KW - Neoplasm Recurrence, Local KW - Adolescent KW - Male KW - Female KW - Child, Preschool KW - Vitamin A -- therapeutic use KW - Carcinoma, Non-Small-Cell Lung -- prevention & control KW - Vitamin A -- adverse effects KW - Lung Neoplasms -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78699729?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.atitle=Lung+cancer+chemoprevention+with+retinol+palmitate.+Preliminary+data+from+a+randomized+trial+on+stage+Ia+non+small-cell+lung+cancer.&rft.au=Pastorino%2C+U%3BSoresi%2C+E%3BClerici%2C+M%3BChiesa%2C+G%3BBelloni%2C+P+A%3BOngari%2C+M%3BValente%2C+M%3BRavasi%2C+G&rft.aulast=Pastorino&rft.aufirst=U&rft.date=1988-01-01&rft.volume=27&rft.issue=6b&rft.spage=773&rft.isbn=&rft.btitle=&rft.title=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.issn=0284186X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-04-26 N1 - Date created - 1989-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Rat liver cytosolic protein changes after ethanol exposure studied by two-dimensional electrophoresis. AN - 78696148; 3234333 AB - Rats were fed liquid food containing ethanol in concentrations ranging from 1-5% for 13 weeks. Livers were removed for histopathology and the liver cytosolic protein fraction was prepared and used for two-dimensional gel electrophoresis (2D-PAGE). Polypeptides were visualized by silver staining. Scanning was made for estimation of the relative abundance of protein in each polypeptide spot in the gels and for comparison between rats. Visual inspection and scanning of gels with the stained polypeptide spots obtained after equilibrium isoelectric focusing and non-equilibrium pH gradient electrophoresis revealed that: 1) within the control rat and ethanol-treated rat livers the numbers of polypeptide spots detected using isoelectric focusing in the first dimension were approximately 500 and for non-equilibrium pH gradient electrophoresis 400; 2) in the control group the variation in the estimated amount of protein in each spot was remarkably small; 3) pronounced differences in the relative abundance of protein in several of the spots was observed in the ethanol-exposed rats as compared to controls. Dose-response relations and possible causes for the effects of ethanol are discussed. JF - Electrophoresis AU - Wirth, P J AU - Vesterberg, O AD - Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, MD. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 47 EP - 53 VL - 9 IS - 1 SN - 0173-0835, 0173-0835 KW - Proteins KW - 0 KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Electrophoresis, Gel, Two-Dimensional KW - Image Processing, Computer-Assisted KW - Male KW - Cytosol -- metabolism KW - Liver -- drug effects KW - Ethanol -- pharmacology KW - Cytosol -- drug effects KW - Liver -- metabolism KW - Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78696148?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Electrophoresis&rft.atitle=Rat+liver+cytosolic+protein+changes+after+ethanol+exposure+studied+by+two-dimensional+electrophoresis.&rft.au=Wirth%2C+P+J%3BVesterberg%2C+O&rft.aulast=Wirth&rft.aufirst=P&rft.date=1988-01-01&rft.volume=9&rft.issue=1&rft.spage=47&rft.isbn=&rft.btitle=&rft.title=Electrophoresis&rft.issn=01730835&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-04-27 N1 - Date created - 1989-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Acute respiratory failure following pharmacologically induced hyperventilation: an experimental animal study. AN - 78686120; 3230208 AB - The pulmonary effects of hyperventilation following infusion of sodium salicylate into the cisterna magna was studied in 16 spontaneously breathing adult sheep. We found a fall in PaO2, a decrease in the static compliance of the respiratory system, abnormal chest roentgenographic films, and grossly abnormal lungs following 3.5 to 13 h of hyperventilation. A control group of 15 sheep (10 sheep similarly injected with sodium salicylate, but then sedated and paralyzed and ventilated at normal tidal volume and respiratory rate on a mechanical ventilator, and 5 sheep infused with saline alone and breathing spontaneously) showed no pulmonary or arterial blood gas abnormalities. We conclude that prolonged hyperventilation under the conditions of this experiment precipitated events that resulted in acute lung injury. JF - Intensive care medicine AU - Mascheroni, D AU - Kolobow, T AU - Fumagalli, R AU - Moretti, M P AU - Chen, V AU - Buckhold, D AD - National Institutes of Health, Laboratory of Technical Development, Bethesda, Maryland. Y1 - 1988 PY - 1988 DA - 1988 SP - 8 EP - 14 VL - 15 IS - 1 SN - 0342-4642, 0342-4642 KW - Sodium Salicylate KW - WIQ1H85SYP KW - Index Medicus KW - Animals KW - Sodium Salicylate -- adverse effects KW - Sheep KW - Respiration, Artificial KW - Lung Volume Measurements KW - Hyperventilation -- chemically induced KW - Hyperventilation -- complications KW - Respiratory Insufficiency -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78686120?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Intensive+care+medicine&rft.atitle=Acute+respiratory+failure+following+pharmacologically+induced+hyperventilation%3A+an+experimental+animal+study.&rft.au=Mascheroni%2C+D%3BKolobow%2C+T%3BFumagalli%2C+R%3BMoretti%2C+M+P%3BChen%2C+V%3BBuckhold%2C+D&rft.aulast=Mascheroni&rft.aufirst=D&rft.date=1988-01-01&rft.volume=15&rft.issue=1&rft.spage=8&rft.isbn=&rft.btitle=&rft.title=Intensive+care+medicine&rft.issn=03424642&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-04-17 N1 - Date created - 1989-04-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Human bronchial epithelial cells neoplastically transformed by v-Ki-ras: altered response to inducers of terminal squamous differentiation. AN - 78679115; 3067190 AB - Many human bronchial adenocarcinomas have been shown to contain an activated Ki-ras oncogene (Rodenhuis et al., N. Engl. J. Med. 317 929-935, 1987). To test the hypothesis that activated Ki-ras may be causally related to human bronchial carcinogenesis, v-Ki-ras oncogene was transferred into an established human bronchial epithelial cell line, BEAS-2B, by infection with Kirsten murine sarcoma virus (Ki-MSV) or by transfection with a plasmid containing the transforming region of Ki-MSV. These cells formed poorly differentiated adenocarcinomas in athymic nude mice. Cell lines established from these tumors expressed v-Ki-ras p21 protein and were highly tumorigenic. Whereas serum or transforming growth factor beta 1 induced the BEAS-2B cells at clonal density to undergo growth arrest and squamous differentiation, BEAS-2B cells containing activated ras genes were unaffected by transforming growth factor beta 1 and were mitogenically stimulated by serum. JF - Oncogene research AU - Reddel, R R AU - Ke, Y AU - Kaighn, M E AU - Malan-Shibley, L AU - Lechner, J F AU - Rhim, J S AU - Harris, C C AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 401 EP - 408 VL - 3 IS - 4 SN - 0890-6467, 0890-6467 KW - Growth Substances KW - 0 KW - Transforming Growth Factors KW - 76057-06-2 KW - Index Medicus KW - Animals KW - Tumor Cells, Cultured KW - Epithelial Cells KW - Humans KW - Cell Division -- drug effects KW - Growth Substances -- blood KW - Bronchial Neoplasms -- genetics KW - Mice KW - Gene Expression Regulation KW - Transforming Growth Factors -- pharmacology KW - Bronchial Neoplasms -- pathology KW - Genes, ras KW - Cell Transformation, Neoplastic -- pathology KW - Bronchi -- cytology KW - Neoplasms, Experimental -- genetics KW - Neoplasms, Experimental -- pathology KW - Cell Differentiation -- drug effects KW - Cell Transformation, Neoplastic -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78679115?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene+research&rft.atitle=Human+bronchial+epithelial+cells+neoplastically+transformed+by+v-Ki-ras%3A+altered+response+to+inducers+of+terminal+squamous+differentiation.&rft.au=Reddel%2C+R+R%3BKe%2C+Y%3BKaighn%2C+M+E%3BMalan-Shibley%2C+L%3BLechner%2C+J+F%3BRhim%2C+J+S%3BHarris%2C+C+C&rft.aulast=Reddel&rft.aufirst=R&rft.date=1988-01-01&rft.volume=3&rft.issue=4&rft.spage=401&rft.isbn=&rft.btitle=&rft.title=Oncogene+research&rft.issn=08906467&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-29 N1 - Date created - 1989-03-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Negative regulation of c-myc transcription involves myc family proteins. AN - 78679052; 2976141 AB - Expression of the c-myc gene is suppressed in NIH 3T3 mouse fibroblast cells infected with recombinant retroviruses expressing high levels of v-myc (10-fold greater than those of c-myc). Suppression of steady state levels of c-myc mRNA occurred at least in part at the level of transcription from c-myc promoters P1 and P2, and involved v-myc protein since cells infected with constructs containing frameshifts and deletions in v-myc had normal levels of c-myc mRNA and protein. Suppression of c-myc expression was also observed in fibroblasts transfected with a N-myc expression vector and in fibroblasts infected with a c-myc retrovirus. These findings establish that v-myc protein is involved either directly or indirectly in a regulatory circuit which represses c-myc proto-oncogene transcription. Feedback regulation of c-myc transcription may be relevant in establishing the lineage specific expression of myc family proto-oncogenes. Reduced steady state levels of c-myc mRNA were also observed in NIH 3T3 cells infected with 12S and 13S EIA recombinant retroviruses suggesting that the exogenous oncogene of adenovirus, EIA, can alleviate the requirement of myc for cell growth and may also share transcriptional target genes. JF - Oncogene research AU - Cleveland, J L AU - Huleihel, M AU - Bressler, P AU - Siebenlist, U AU - Akiyama, L AU - Eisenman, R N AU - Rapp, U R AD - Laboratory of Viral Carcinogenesis, National Cancer Institute, FCRF, Frederick, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 357 EP - 375 VL - 3 IS - 4 SN - 0890-6467, 0890-6467 KW - Adenovirus Early Proteins KW - 0 KW - Oncogene Proteins, Viral KW - Proto-Oncogene Proteins KW - Proto-Oncogene Proteins c-myc KW - RNA, Messenger KW - Transcription Factors KW - Index Medicus KW - Animals KW - Blotting, Northern KW - DNA Mutational Analysis KW - Transcription, Genetic KW - Mice KW - RNA, Messenger -- genetics KW - Cell Line KW - Transcription Factors -- physiology KW - Oncogenes KW - Oncogene Proteins, Viral -- genetics KW - Oncogene Proteins, Viral -- physiology KW - Gene Expression Regulation KW - Proto-Oncogene Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78679052?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene+research&rft.atitle=Negative+regulation+of+c-myc+transcription+involves+myc+family+proteins.&rft.au=Cleveland%2C+J+L%3BHuleihel%2C+M%3BBressler%2C+P%3BSiebenlist%2C+U%3BAkiyama%2C+L%3BEisenman%2C+R+N%3BRapp%2C+U+R&rft.aulast=Cleveland&rft.aufirst=J&rft.date=1988-01-01&rft.volume=3&rft.issue=4&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Oncogene+research&rft.issn=08906467&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-29 N1 - Date created - 1989-03-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The twin method in the study of vulnerability to drug abuse. AN - 78676590; 3147397 JF - NIDA research monograph AU - Pickens, R W AU - Svikis, D S AD - Division of Clinica Research, National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 41 EP - 51 VL - 89 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - Disease Susceptibility KW - Twins, Monozygotic KW - Humans KW - Adult KW - Twins, Dizygotic KW - Male KW - Female KW - Diseases in Twins KW - Alcoholism -- genetics KW - Substance-Related Disorders -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78676590?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=The+twin+method+in+the+study+of+vulnerability+to+drug+abuse.&rft.au=Pickens%2C+R+W%3BSvikis%2C+D+S&rft.aulast=Pickens&rft.aufirst=R&rft.date=1988-01-01&rft.volume=89&rft.issue=&rft.spage=41&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-31 N1 - Date created - 1989-03-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The use of second-order schedules to study the influence of environmental stimuli on drug-seeking behavior. AN - 78676300; 3147382 JF - NIDA research monograph AU - Schindler, C W AU - Katz, J L AU - Goldberg, S R AD - Preclinical Pharmacology Branch, National Institute on Drug Abuse, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 180 EP - 195 VL - 84 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - Animals KW - Adaptation, Psychological KW - Humans KW - Disease Models, Animal KW - Substance-Related Disorders -- therapy KW - Conditioning (Psychology) KW - Substance-Related Disorders -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78676300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=The+use+of+second-order+schedules+to+study+the+influence+of+environmental+stimuli+on+drug-seeking+behavior.&rft.au=Schindler%2C+C+W%3BKatz%2C+J+L%3BGoldberg%2C+S+R&rft.aulast=Schindler&rft.aufirst=C&rft.date=1988-01-01&rft.volume=84&rft.issue=&rft.spage=180&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-31 N1 - Date created - 1989-03-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Potential interactions between GABAb and cholinergic systems: baclofen augments scopolamine-induced performance deficits in the eight-arm radial maze. AN - 78672472; 3147471 AB - Sixteen male Fischer-344N rats were trained on a eight-arm radial maze task for food reinforcement. The effects of various doses of baclofen (1.25 or 2.50 mg/kg) and scopolamine (0.188, 0.375, and 0.750 mg/kg) were determined alone and in combination. Relative to vehicle controls, baclofen alone did not affect performance in the radial arm maze (number correct in the first eight responses, total errors) or the time required to complete the maze. Scopolamine alone decreased the number of correct responses in the first eight arm choices, while increasing both the number of errors and the time necessary to complete the maze. When the two drugs were co-administered, baclofen had no effect on the number of errors or time required to complete the maze in the presence of scopolamine; however, in combination with the high dose of scopolamine, it significantly increased the number of errors made during the first eight choices. Baclofen thus can exacerbate some radial arm maze dificits produced by an anticholinergic drug. In a subsequent experiment to test the interaction between scopolamine and baclofen using a nonlearned behavior, baclofen (1.25 and 2.5 mg/kg) did not affect motor activity, whereas all doses of scopolamine (0.188-0.75 mg/kg) increased activity. The higher dose of baclofen attenuated scopolamine-induced hypermotility by 50%, but the lower dose of baclofen was not effective. These data demonstrate pharmacological interactions between baclofen, a drug used clinically for spaticity, and a drug having anticholinergic properties. JF - Psychopharmacology AU - Sidel, E S AU - Tilson, H A AU - McLamb, R L AU - Wilson, W A AU - Swartzwelder, H S AD - Laboratory of Molecular and Integrative Neuroscience, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 116 EP - 120 VL - 96 IS - 1 SN - 0033-3158, 0033-3158 KW - Scopolamine Hydrobromide KW - 451IFR0GXB KW - gamma-Aminobutyric Acid KW - 56-12-2 KW - Baclofen KW - H789N3FKE8 KW - Index Medicus KW - Rats KW - Conditioning, Operant -- drug effects KW - Animals KW - Rats, Inbred F344 KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Motor Activity -- drug effects KW - Male KW - Scopolamine Hydrobromide -- pharmacology KW - Psychomotor Performance -- drug effects KW - Parasympathetic Nervous System -- drug effects KW - gamma-Aminobutyric Acid -- physiology KW - Baclofen -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78672472?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Potential+interactions+between+GABAb+and+cholinergic+systems%3A+baclofen+augments+scopolamine-induced+performance+deficits+in+the+eight-arm+radial+maze.&rft.au=Sidel%2C+E+S%3BTilson%2C+H+A%3BMcLamb%2C+R+L%3BWilson%2C+W+A%3BSwartzwelder%2C+H+S&rft.aulast=Sidel&rft.aufirst=E&rft.date=1988-01-01&rft.volume=96&rft.issue=1&rft.spage=116&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-04-05 N1 - Date created - 1989-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Rearing paradigm in a nonhuman primate affects response to beta-CCE challenge. AN - 78671805; 3147480 AB - Two groups of socially housed rhesus monkeys were raised under conditions differing in the degree of control or mastery over appetitive stimuli (food, water, and treats) in the 1st year of life. At age 18-24 months, a benzodiazepine receptor inverse agonist, beta-carboline-3-carboxylic acid ethyl ester (beta-CCE), was administered to both social groups to investigate the effects of differential rearing on the response to a putative anxiogenic drug. In both groups beta-CCE was followed by behavioral activation with a profile suggesting increases in aggression rather than fear or anxiety. A group by drug analysis revealed that the increase in aggression was observed predominantly in the subjects reared with mastery during the 1st year. Plasma cortisol increased in both groups, but its increase was greater in the yoked subjects. These results suggest that early experience with controllability may have long term consequences which can be revealed by challenge with beta-CCE. JF - Psychopharmacology AU - Insel, T R AU - Scanlan, J AU - Champoux, M AU - Suomi, S J AD - Laboratory of Clinical Science, NIMH, Poolesville, MD 20837. Y1 - 1988 PY - 1988 DA - 1988 SP - 81 EP - 86 VL - 96 IS - 1 SN - 0033-3158, 0033-3158 KW - Carbolines KW - 0 KW - beta-carboline-3-carboxylic acid ethyl ester KW - 74214-62-3 KW - Hydrocortisone KW - WI4X0X7BPJ KW - Index Medicus KW - Conditioning, Operant -- drug effects KW - Animals KW - Aggression -- drug effects KW - Drug Interactions KW - Reinforcement Schedule KW - Fear -- drug effects KW - Motor Activity -- drug effects KW - Macaca mulatta KW - Male KW - Hydrocortisone -- blood KW - Female KW - Behavior, Animal -- drug effects KW - Carbolines -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78671805?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Rearing+paradigm+in+a+nonhuman+primate+affects+response+to+beta-CCE+challenge.&rft.au=Insel%2C+T+R%3BScanlan%2C+J%3BChampoux%2C+M%3BSuomi%2C+S+J&rft.aulast=Insel&rft.aufirst=T&rft.date=1988-01-01&rft.volume=96&rft.issue=1&rft.spage=81&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-04-05 N1 - Date created - 1989-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The use of oligonucleotide-directed mutagenesis to probe molecular mechanisms of mutagenesis. AN - 78670876; 3226920 JF - Nucleic acids symposium series AU - Kunkel, T A AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 43 IS - 19 SN - 0261-3166, 0261-3166 KW - Oligonucleotide Probes KW - 0 KW - Index Medicus KW - Genetic Engineering -- methods KW - Mutation KW - DNA Replication UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78670876?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+symposium+series&rft.atitle=The+use+of+oligonucleotide-directed+mutagenesis+to+probe+molecular+mechanisms+of+mutagenesis.&rft.au=Kunkel%2C+T+A&rft.aulast=Kunkel&rft.aufirst=T&rft.date=1988-01-01&rft.volume=&rft.issue=19&rft.spage=43&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+symposium+series&rft.issn=02613166&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-29 N1 - Date created - 1989-03-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Genetic vulnerability to drug abuse. AN - 78667500; 3147389 JF - NIDA research monograph AU - Pickens, R W AU - Svikis, D S AD - Division of Clinical Research, National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 8 VL - 89 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - Disease Susceptibility KW - Humans KW - Substance-Related Disorders -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78667500?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Genetic+vulnerability+to+drug+abuse.&rft.au=Pickens%2C+R+W%3BSvikis%2C+D+S&rft.aulast=Pickens&rft.aufirst=R&rft.date=1988-01-01&rft.volume=89&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-31 N1 - Date created - 1989-03-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Current approaches to the adoptive immunotherapy of cancer. AN - 78666972; 3265581 AB - Interleukin 2 has provided the means of generating activated lymphocytes from the tumor-bearing host which show in vitro anti-tumor activity. These cells can be derived either from a ubiquitous null cell precursor population, exhibiting promiscuous lysis of nearly all tumors after culture in IL2 (ie. the LAK cell) or from a T cell precursor population infiltrating tumors and showing some relative specificity for lysis of the autologous tumor following activation (ie. TIL). When utilized in murine cellular adoptive immunotherapy models, both LAK cells and TIL are able to display marked anti-tumor effects against established micrometastatic tumor. LAK cells have been subsequently employed in adoptive immunotherapy against metastatic human tumors and in a limited number of cases have been able to mediate the regression of advanced cancers of differing histologies. The role of adoptive immunotherapy as standard therapy of human tumors will depend on refinement of the methodology and progress toward increased efficacy and reduced toxicity. TIL may possess theoretical advantages in these areas and efforts are currently proceeding to evaluate these new anti-tumor reagents in the the treatment of patients with cancer. JF - Advances in experimental medicine and biology AU - Yang, J C AU - Rosenberg, S A AD - Surgery Branch, National Cancer Institute Bethesda, Md. Y1 - 1988 PY - 1988 DA - 1988 SP - 459 EP - 467 VL - 233 SN - 0065-2598, 0065-2598 KW - Interleukin-2 KW - 0 KW - Index Medicus KW - Lymphocyte Activation KW - Cytotoxicity, Immunologic KW - Animals KW - Liver Neoplasms -- therapy KW - Lung Neoplasms -- secondary KW - Humans KW - Neoplasm Metastasis KW - Lung Neoplasms -- therapy KW - Mice KW - Liver Neoplasms -- secondary KW - Neoplasms, Experimental -- immunology KW - Neoplasms, Experimental -- therapy KW - Interleukin-2 -- therapeutic use KW - Immunization, Passive KW - Neoplasms, Experimental -- pathology KW - Melanoma -- immunology KW - Killer Cells, Natural -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78666972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+experimental+medicine+and+biology&rft.atitle=Current+approaches+to+the+adoptive+immunotherapy+of+cancer.&rft.au=Yang%2C+J+C%3BRosenberg%2C+S+A&rft.aulast=Yang&rft.aufirst=J&rft.date=1988-01-01&rft.volume=233&rft.issue=&rft.spage=459&rft.isbn=&rft.btitle=&rft.title=Advances+in+experimental+medicine+and+biology&rft.issn=00652598&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-22 N1 - Date created - 1989-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The measurement of D,L-2,3-butanediol in controls and patients with alcoholic cirrhosis. AN - 78666448; 3223433 AB - Plasma D,L-2,3-butanediol was measured in 53 controls and 50 patients with alcoholic cirrhosis, none of whom had measurable amounts of blood ethanol. Thirteen of 50 samples from patients with alcoholic cirrhosis had measurable D,L-2,3-butanediol. (range less than 5-154 microM). In one patient with alcoholic cirrhosis who had been abstinent from ethanol for over 5 years plasma levels of D,L-2,3-butanediol ranged between 154 and 211 microM over a one-year period. Only one of the 53 control subjects had detectable levels of D,L-2,3-butanediol. Although it has previously been reported that 2,3-butanediol is present in alcoholics consuming distilled spirits (Rutstein et al. (1983) Lancet ii, 534), this is the first report of the persistent presence of these compounds in alcoholics in the absence of ethanol. Clearly in abstinent alcoholics the presence of 2,3-butanediol is not due to the ingestion of undistilled spirits nor is it likely to arise directly from the metabolic products of ethanol. The presence of D,L-2,3-butanediol in patients with alcoholic cirrhosis and its absence in control subjects suggests that this compound may be a marker of some forms for alcoholism. JF - Advances in alcohol & substance abuse AU - Casazza, J P AU - Freitas, J AU - Stambuk, D AU - Morgan, M Y AU - Veech, R L AD - Laboratory of Metabolism and Molecular Biology, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852. Y1 - 1988 PY - 1988 DA - 1988 SP - 33 EP - 35 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Biomarkers KW - 0 KW - Butylene Glycols KW - 2,3-butylene glycol KW - 45427ZB5IJ KW - Index Medicus KW - Humans KW - Butylene Glycols -- blood KW - Liver Cirrhosis, Alcoholic -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78666448?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=The+measurement+of+D%2CL-2%2C3-butanediol+in+controls+and+patients+with+alcoholic+cirrhosis.&rft.au=Casazza%2C+J+P%3BFreitas%2C+J%3BStambuk%2C+D%3BMorgan%2C+M+Y%3BVeech%2C+R+L&rft.aulast=Casazza&rft.aufirst=J&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=33&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Acute effects of ethanol on motor performance and movement-related brain potentials. AN - 78666134; 3223435 AB - The acute effects of ethanol on skilled motor functions were examined in male social drinkers, under four doses ranging from 0 (placebo) to 1.05 g/kg lean body weight. The movement entailed a forewarned choice transitive motion of the arm and hand, aimed at a flanking target. Performance measures disclosed only small effects of ethanol on speed and accuracy of movement. The simultaneously-recorded movement-related brain potentials disclosed decreased involvement of frontal and posterior brain areas, suggesting that ethanol disrupted the planning and regulation of movement despite the overall preservation of reaction speed. JF - Advances in alcohol & substance abuse AU - Rohrbaugh, J W AU - Stapleton, J M AU - Frowein, H W AU - Adinoff, B AU - Varner, J L AU - Lane, E A AU - Eckardt, M J AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 53 EP - 57 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - Reaction Time -- drug effects KW - Humans KW - Male KW - Movement -- drug effects KW - Ethanol -- adverse effects KW - Psychomotor Performance -- drug effects KW - Electrophysiology -- drug effects KW - Contingent Negative Variation -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78666134?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Acute+effects+of+ethanol+on+motor+performance+and+movement-related+brain+potentials.&rft.au=Rohrbaugh%2C+J+W%3BStapleton%2C+J+M%3BFrowein%2C+H+W%3BAdinoff%2C+B%3BVarner%2C+J+L%3BLane%2C+E+A%3BEckardt%2C+M+J%3BLinnoila%2C+M&rft.aulast=Rohrbaugh&rft.aufirst=J&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=53&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Treatment of alcoholic organic brain syndrome with the serotonin reuptake inhibitor fluvoxamine: a preliminary study. AN - 78666007; 2464912 AB - The chronic effects of fluvoxamine (200 mg per day for 4 weeks) were studied in ten alcoholic organic brain syndrome patients in a double-blind cross-over design. Complete neuropsychological evaluation was performed as well as measurement of neurochemical changes in CSF. Fluvoxamine produced a small but significant improvement in memory performance. An analysis of fluvoxamine minus placebo difference scores showed a significant correlation between memory functioning and CSF 5HIAA levels. Alcohol amnestic syndrome patients who had the highest blood levels of fluvoxamine demonstrated the largest changes in CSF 5HIAA and improvement in memory performance under fluvoxamine. These findings implicate a role of serotonergic mechanisms in alcoholic organic brain syndrome and suggest that with individual titration of the drug dose, fluvoxamine might be a clinically useful agent in the treatment of this syndrome. JF - Advances in alcohol & substance abuse AU - Stapleton, J M AU - Eckardt, M J AU - Martin, P AU - Adinoff, B AU - Roehrich, L AU - Bone, G AU - Rubinow, D AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 47 EP - 51 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Oximes KW - 0 KW - Ethanol KW - 3K9958V90M KW - Hydroxyindoleacetic Acid KW - 54-16-0 KW - Fluvoxamine KW - O4L1XPO44W KW - Index Medicus KW - Double-Blind Method KW - Humans KW - Hydroxyindoleacetic Acid -- cerebrospinal fluid KW - Aged KW - Middle Aged KW - Dementia -- chemically induced KW - Dementia -- drug therapy KW - Alcohol Amnestic Disorder -- drug therapy KW - Ethanol -- adverse effects KW - Substance-Related Disorders -- drug therapy KW - Oximes -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78666007?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Treatment+of+alcoholic+organic+brain+syndrome+with+the+serotonin+reuptake+inhibitor+fluvoxamine%3A+a+preliminary+study.&rft.au=Stapleton%2C+J+M%3BEckardt%2C+M+J%3BMartin%2C+P%3BAdinoff%2C+B%3BRoehrich%2C+L%3BBone%2C+G%3BRubinow%2C+D%3BLinnoila%2C+M&rft.aulast=Stapleton&rft.aufirst=J&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=47&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Circadian rhythms of cortisol during alcohol withdrawal. AN - 78665951; 3223434 AB - The authors have investigated the function of the hypothalamic-pituitary-adrenocortical (HPA) axis during and after withdrawal from alcohol. 24 hour rhythms of cortisol were abnormal in that elevated levels were seen throughout the day in patients with moderate to severe, but not mild, withdrawal. This abnormality of circadian secretion of cortisol, which is similar to that seen in Cushing's syndrome and post-operative trauma, returned to normal after a period of one week of abstinence on their in-patient ward. Such excessive secretion of cortisol may explain some of the complications of chronic alcoholism. JF - Advances in alcohol & substance abuse AU - Risher-Flowers, D AU - Adinoff, B AU - Ravitz, B AU - Bone, G H AU - Martin, P R AU - Nutt, D AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 37 EP - 41 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Ethanol KW - 3K9958V90M KW - Hydrocortisone KW - WI4X0X7BPJ KW - Index Medicus KW - Humans KW - Adult KW - Middle Aged KW - Time Factors KW - Male KW - Ethanol -- adverse effects KW - Circadian Rhythm KW - Substance Withdrawal Syndrome -- blood KW - Hydrocortisone -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78665951?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Circadian+rhythms+of+cortisol+during+alcohol+withdrawal.&rft.au=Risher-Flowers%2C+D%3BAdinoff%2C+B%3BRavitz%2C+B%3BBone%2C+G+H%3BMartin%2C+P+R%3BNutt%2C+D%3BLinnoila%2C+M&rft.aulast=Risher-Flowers&rft.aufirst=D&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=37&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - RO 15-4513 and its interaction with ethanol. AN - 78664328; 2851930 AB - It has recently been claimed that RO 15-4513 selectively opposes some of the behavioral actions of ethanol. Our studies on the intrinsic effects of this compound have shown it to be proconvulsant and to reduce exploratory behavior in mice. In these respects RO 15-4513 resembles a benzodiazepine receptor partial inverse agonist. Such intrinsic actions may well explain its alcohol-antagonizing properties, and argue against its potential in humans. In addition to partially reversing the effects of ethanol, RO 15-4513 also partially reverses the behavioral effect of a barbiturate and completely reverses the effects of a benzodiazepine. JF - Advances in alcohol & substance abuse AU - Lister, R G AU - Nutt, D J AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 119 EP - 123 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Azides KW - 0 KW - Receptors, GABA-A KW - Benzodiazepines KW - 12794-10-4 KW - Ethanol KW - 3K9958V90M KW - Ro 15-4513 KW - 91917-65-6 KW - Pentobarbital KW - I4744080IR KW - Index Medicus KW - Rats KW - Saimiri KW - Animals KW - Drug Interactions KW - Receptors, GABA-A -- drug effects KW - Mice KW - Pentobarbital -- pharmacology KW - Benzodiazepines -- antagonists & inhibitors KW - Ethanol -- pharmacology KW - Azides -- pharmacology KW - Benzodiazepines -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78664328?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=RO+15-4513+and+its+interaction+with+ethanol.&rft.au=Lister%2C+R+G%3BNutt%2C+D+J&rft.aulast=Lister&rft.aufirst=R&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=119&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - GC/MS assay of prostaglandins in cerebrospinal fluid from humans and monkeys. AN - 78664245; 3223427 AB - The objective of this project has been to develop a sensitive and specific assay for prostaglandins in human cerebrospinal fluid (CSF) from patients with alcoholism and appropriate controls using gas chromatography/mass spectrometry. This study was initiated because numerous literature reports strongly suggest that a relationship exists between ethanol's central nervous system effects and the central production of prostaglandins. In both human and animal studies, administration of prostaglandin synthesis inhibitors prior to administration of ethanol attenuated central nervous system effects of ethanol. Samples from alcoholics after a three week period of abstinence and normals contained none of the measured prostaglandins (PGE2, PGE1, PGF1a, PGF2a, 6-keto-PGF1a) at a concentration more than twice the limit of quantification (3 pg/mL CSF). Comparison of GC/MS and radioimmunoassay methods provided further validation for these results. Literature reports of much higher levels of prostaglandins in normal controls, i.e., tens to hundreds of pg/mL CSF, appear to be incorrect. Examination of monkey CSF provided a positive control, since several of the prostaglandins were easily quantifiable in these samples. JF - Advances in alcohol & substance abuse AU - Yergey, J A AU - Salem, N AU - Karanian, J W AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 125 EP - 129 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Prostaglandins KW - 0 KW - 6-Ketoprostaglandin F1 alpha KW - 58962-34-8 KW - Dinoprost KW - B7IN85G1HY KW - Dinoprostone KW - K7Q1JQR04M KW - Index Medicus KW - Animals KW - Humans KW - Dinoprostone -- cerebrospinal fluid KW - Gas Chromatography-Mass Spectrometry KW - 6-Ketoprostaglandin F1 alpha -- cerebrospinal fluid KW - Macaca mulatta KW - Dinoprost -- cerebrospinal fluid KW - Radioimmunoassay KW - Prostaglandins -- cerebrospinal fluid KW - Alcoholism -- cerebrospinal fluid UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78664245?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=GC%2FMS+assay+of+prostaglandins+in+cerebrospinal+fluid+from+humans+and+monkeys.&rft.au=Yergey%2C+J+A%3BSalem%2C+N%3BKaranian%2C+J+W%3BLinnoila%2C+M&rft.aulast=Yergey&rft.aufirst=J&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=125&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alpha-2-adrenoceptor function in alcoholics. AN - 78664153; 2851932 AB - Alpha-2-adrenoceptor function has been assessed using the iv clonidine challenge test. During withdrawal clonidine effects on blood pressure, sedation and body temperature were blunted compared with the abstinent state and healthy controls. In contrast the growth hormone response was blunted in both the withdrawing and abstinent alcoholics. These findings suggest that a deficit of alpha-2-inhibitory control is a feature of withdrawal and, in the case of the endocrine response, may persist for some time. JF - Advances in alcohol & substance abuse AU - Nutt, D J AU - Glue, P AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 43 EP - 46 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Receptors, Adrenergic, alpha KW - 0 KW - Ethanol KW - 3K9958V90M KW - Growth Hormone KW - 9002-72-6 KW - Clonidine KW - MN3L5RMN02 KW - Index Medicus KW - Body Temperature -- drug effects KW - Growth Hormone -- physiology KW - Humans KW - Blood Pressure -- drug effects KW - Ethanol -- adverse effects KW - Substance Withdrawal Syndrome -- physiopathology KW - Receptors, Adrenergic, alpha -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78664153?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Alpha-2-adrenoceptor+function+in+alcoholics.&rft.au=Nutt%2C+D+J%3BGlue%2C+P&rft.aulast=Nutt&rft.aufirst=D&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=43&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neurotransmitters and alcoholism: methodological issues. AN - 78664052; 2464911 AB - This short review examines recent findings on neurochemical differences between alcoholics and various control populations. Particular emphasis is given to clinical variables which affect concentrations of neurotransmitter metabolites in the cerebrospinal fluid, and which have to be controlled in order to make meaningful comparisons between various diagnostic groups. The review focuses on two of the major monoamine transmitters, serotonin and norepinephrine, and excludes neurotransmitters and modulators such as dopamine, acetylcholine, peptides, prostaglandins, amino acids and purines, since their significance to alcoholism is currently less well understood. JF - Advances in alcohol & substance abuse AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 17 EP - 24 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Serotonin KW - 333DO1RDJY KW - Hydroxyindoleacetic Acid KW - 54-16-0 KW - Norepinephrine KW - X4W3ENH1CV KW - Index Medicus KW - Humans KW - Hydroxyindoleacetic Acid -- cerebrospinal fluid KW - Norepinephrine -- physiology KW - Serotonin -- physiology KW - Alcoholism -- physiopathology KW - Alcoholism -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78664052?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Neurotransmitters+and+alcoholism%3A+methodological+issues.&rft.au=Linnoila%2C+M&rft.aulast=Linnoila&rft.aufirst=M&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Brain imaging in alcoholic patients. AN - 78663504; 3066195 AB - Imaging in vivo aspects of brain structure and function hold great promise for the study of alcoholism. Computerized axial tomography and magnetic resonance imaging have been used successfully to demonstrate structural abnormalities in alcoholic patients. Positron emission tomography and topographic images of electrical and magnetic activity are useful measures of brain function that could be applied more rigorously to the study of alcoholism. Interrelating various types of imaging data is an important area that is still in the developmental stage. JF - Advances in alcohol & substance abuse AU - Eckardt, M J AU - Rohrbaugh, J W AU - Rio, D AU - Rawlings, R R AU - Coppola, R AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 59 EP - 71 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Index Medicus KW - Magnetic Resonance Imaging KW - Magnetoencephalography KW - Humans KW - Electroencephalography KW - Tomography, X-Ray Computed KW - Tomography, Emission-Computed KW - Cerebrovascular Circulation KW - Alcoholism -- diagnosis KW - Brain -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78663504?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Brain+imaging+in+alcoholic+patients.&rft.au=Eckardt%2C+M+J%3BRohrbaugh%2C+J+W%3BRio%2C+D%3BRawlings%2C+R+R%3BCoppola%2C+R&rft.aulast=Eckardt&rft.aufirst=M&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=59&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The aminopyrine breath test for the evaluation of liver function in alcoholic patients: drug pharmacokinetics and environmental factors. AN - 78663440; 3146921 AB - Drug pharmacokinetics and environmental factors contribute to the selection of an ideal drug substrate for the determination of liver function via the carbon dioxide breath test. An ideal drug should be rapidly absorbed, and have an hepatic extraction ratio between 0.2 and 0.5. Its metabolism should not be induced by ethanol or be affected by cigarette smoking. The relative promise of caffeine and methacetin are compared to aminopyrine. JF - Advances in alcohol & substance abuse AU - Lane, E A AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 25 EP - 32 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Acetamides KW - 0 KW - Aminopyrine KW - 01704YP3MO KW - methacetin KW - 13E468TFHP KW - Carbon Dioxide KW - 142M471B3J KW - Caffeine KW - 3G6A5W338E KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - Smoking -- physiopathology KW - Carbon Dioxide -- analysis KW - Caffeine -- pharmacokinetics KW - Ethanol -- pharmacology KW - Humans KW - Acetamides -- pharmacokinetics KW - Aminopyrine -- pharmacokinetics KW - Liver Function Tests KW - Liver Diseases, Alcoholic -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78663440?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=The+aminopyrine+breath+test+for+the+evaluation+of+liver+function+in+alcoholic+patients%3A+drug+pharmacokinetics+and+environmental+factors.&rft.au=Lane%2C+E+A&rft.aulast=Lane&rft.aufirst=E&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Interactions of 5HT reuptake inhibitors and ethanol in tests of exploration and anxiety. AN - 78663375; 3146920 AB - Treatment with 5HT reuptake inhibitors has been shown to attenuate ethanol consumption in both animals and humans. These experiments investigate in mice the interactions of the 5HT reuptake inhibitors fluoxetine, citalopram and fluvoxamine and the NA uptake inhibitor desipramine with ethanol in the holeboard test and the elevated plusmaze test of anxiety. Ethanol (2.4 g/kg) increased activity both in the holeboard and on the plusmaze, decreased both the number and duration of head-dips in the holeboard, and increased both the percentage time and percentage entries on to the open-arm of the plusmaze (reflecting its anxiolytic properties). On their own, the selective 5HT uptake inhibitors fluoxetine, fluvoxamine, and citalopram and the NA uptake inhibitor desipramine (10-20 mg/kg) did not significantly alter any of the behavioral measures. The only consistent interaction was seen with fluoxetine which reduced ethanol's anxiolytic effects at the 20 mg/kg dose without altering ethanol's effects on exploration or locomotion. The results suggest that the attenuation of ethanol's anxiolytic properties by fluoxetine may not be serotonin related since other 5HT reuptake inhibitors did not show this effect at the doses used. JF - Advances in alcohol & substance abuse AU - Durcan, M J AU - Lister, R G AU - Eckardt, M J AU - Linnoila, M AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 113 EP - 117 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Oximes KW - 0 KW - Serotonin Antagonists KW - Fluoxetine KW - 01K63SUP8D KW - Citalopram KW - 0DHU5B8D6V KW - Ethanol KW - 3K9958V90M KW - Fluvoxamine KW - O4L1XPO44W KW - Desipramine KW - TG537D343B KW - Index Medicus KW - Oximes -- pharmacology KW - Mice, Inbred Strains KW - Animals KW - Fluoxetine -- pharmacology KW - Drug Interactions KW - Desipramine -- pharmacology KW - Exploratory Behavior -- drug effects KW - Citalopram -- pharmacology KW - Anxiety -- physiopathology KW - Motor Activity -- drug effects KW - Mice KW - Serotonin Antagonists -- pharmacology KW - Ethanol -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78663375?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Interactions+of+5HT+reuptake+inhibitors+and+ethanol+in+tests+of+exploration+and+anxiety.&rft.au=Durcan%2C+M+J%3BLister%2C+R+G%3BEckardt%2C+M+J%3BLinnoila%2C+M&rft.aulast=Durcan&rft.aufirst=M&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of chronic ethanol ingestion on mouse brain beta-adrenergic receptors (BAR) and adenylate cyclase. AN - 78663090; 2851934 AB - Previous work showed that chronic ethanol ingestion by C57BL mice resulted in reduced stimulation of cerebral cortical adenylate cyclase (AC) activity by isoproterenol (ISO) and guanine nucleotides (GN). To investigate the mechanism of this change we have assessed the effect of chronic ethanol ingestion on agonist and antagonist binding to BAR in cerebral cortex (mainly beta 1-AR) and cerebellum (mainly beta 2-AR). C57BL mice were fed ethanol in a liquid diet for seven days and were withdrawn for various intervals. Agonist (ISO) binding data were best fit by a two-site model (high and low affinity states) in cortical membranes of control mice. GN induced conversion to a one site model (low affinity state). At the time of withdrawal, ISO binding data in cortical membranes were best fit by a one-site model even in the absence of GN. Antagonist binding was not affected. These results resemble those seen after heterologous desensitization, indicating "uncoupling" of receptor and AC. Control cerebellar ISO binding data were similar to cortical data. Chronic ethanol ingestion, however, did not produce data fit by a one site model in cerebellum. The affinity for ISO of the high affinity state of the BAR was significantly decreased at the time of withdrawal. ISO-stimulated AC-activity in cerebellar membranes was not affected by chronic ethanol ingestion, indicating that, in contrast to cerebral cortex, the cerebellar BAR was not uncoupled from AC. JF - Advances in alcohol & substance abuse AU - Valverius, P AU - Hoffman, P L AU - Tabakoff, B AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852. Y1 - 1988 PY - 1988 DA - 1988 SP - 99 EP - 101 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Guanine Nucleotides KW - 0 KW - Receptors, Adrenergic, beta KW - Adenylyl Cyclases KW - EC 4.6.1.1 KW - Isoproterenol KW - L628TT009W KW - Index Medicus KW - Animals KW - Mice, Inbred C57BL KW - Disease Models, Animal KW - Mice KW - Guanine Nucleotides -- pharmacology KW - Isoproterenol -- pharmacology KW - Receptors, Adrenergic, beta -- metabolism KW - Cerebral Cortex -- metabolism KW - Adenylyl Cyclases -- metabolism KW - Alcoholism -- metabolism KW - Cerebellum -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78663090?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Effects+of+chronic+ethanol+ingestion+on+mouse+brain+beta-adrenergic+receptors+%28BAR%29+and+adenylate+cyclase.&rft.au=Valverius%2C+P%3BHoffman%2C+P+L%3BTabakoff%2C+B&rft.aulast=Valverius&rft.aufirst=P&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=99&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Study design in chronic lymphocytic leukemia. AN - 78662857; 3065736 AB - Recent enthusiasm for clinical trials in chronic lymphocytic leukemia (CLL) has resulted from both an increase in our understanding of the biology of CLL, and the availability of new and active cytotoxic drugs (e.g. 2'-deoxycoformycin [DCF]; fludarabine monophosphate [FAMP]) and the development of interesting biologic agents (e.g. IL-2). To best identify regimens worth pursuing in large scale clinical trials, uniform eligibility, response and toxicity criteria are essential. Standardization will facilitate comparison of results, ensure homogeneity of patient groups on clinical trials, and minimize the arbitrary nature of dose modifications. In 1987, an NCI-sponsored Working Group (NCI-WG) developed Guidelines for US clinical trials for CLL which included specific eligibility, response and toxicity criteria. These will be modified over time as more is learned about the disease. Trials are currently restricted to patients with active B-CLL. Although complete and partial response are carefully defined, alternative systems (e.g. stage shift for partial response) may be tested concurrently and definitions revised if warranted. Similarly, use of the modified Rai staging is encouraged, although other systems may also be studied and compared for clinical relevance. The U.S. Cooperative Oncology Groups and Cancer Centers are participating in a National CLL treatment program following the NCI-WG Guidelines. Phase I and II pilot trials of DCF and FAMP combined with each other or with conventional agents (e.g. FAMP + chlorambucil [CLB] + prednisone [P]; DCF + CLB + P; FAMP + P) are being conducted in previously treated patients. A collaborative phase III trial will compared the most promising regimens with "standard" chemotherapy in untreated patients.(ABSTRACT TRUNCATED AT 250 WORDS) JF - Nouvelle revue francaise d'hematologie AU - Cheson, B D AD - Cancer Therapy Evaluation Program, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 407 EP - 410 VL - 30 IS - 5-6 KW - Index Medicus KW - United States KW - Humans KW - National Institutes of Health (U.S.) KW - Leukemia, Lymphocytic, Chronic, B-Cell -- drug therapy KW - Clinical Trials as Topic -- trends UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78662857?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nouvelle+revue+francaise+d%27hematologie&rft.atitle=Study+design+in+chronic+lymphocytic+leukemia.&rft.au=Cheson%2C+B+D&rft.aulast=Cheson&rft.aufirst=B&rft.date=1988-01-01&rft.volume=30&rft.issue=5-6&rft.spage=407&rft.isbn=&rft.btitle=&rft.title=Nouvelle+revue+francaise+d%27hematologie&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-16 N1 - Date created - 1989-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Adoptive immunotherapy: novel applications of blood cell separators. AN - 78656291; 3065331 AB - Animal models have demonstrated that syngeneic lymphocytes activated ex vivo and infused into animals with experimentally induced tumors can mediate tumor regression. This "adoptive immunotherapy" has been applied to patients with end-stage malignancy refractory to standard therapy. Lymphocytes are collected with the blood cell separator, expanded in culture under the influence of cytokines such as interleukin-2 (IL-2), and reinfused into the patient under conditions similar to those used in the animal models. Studies from several centers using lymphokine-activated killer (LAK) cells, involving more than 300 patients, have shown an overall response rate of greater than 15% and a complete response rate of approximately 10%. Renal cell carcinoma, melanoma, and lymphoma appear to be the cell types that respond best to such therapy. Toxicity in these phase 1 studies has been substantial, related primarily to high doses of intravenous IL-2, and treatment-related deaths have been reported. Adoptive immunotherapy using lymphocytes derived from surgically excised tumors, tumor-infiltrating lymphocytes (TIL), is in the early stages of clinical trials, but this appears to offer a potentially more potent and specific approach than does LAK cell therapy. TIL have been shown to traffic to tumor sites and mediate tumor regression. The mechanisms of adoptive immunotherapy are poorly understood, but blood cell separators and storage technology are playing a critical role in the collection and processing of cells for these research applications. JF - Journal of clinical apheresis AU - Klein, H G AD - Department of Transfusion Medicine, National Institutes of Health, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 198 EP - 202 VL - 4 IS - 4 SN - 0733-2459, 0733-2459 KW - Index Medicus KW - T-Lymphocytes, Cytotoxic -- transplantation KW - Killer Cells, Natural -- transplantation KW - Humans KW - Blood Component Removal -- methods KW - Immunization, Passive -- instrumentation KW - Cell Separation -- instrumentation KW - Immunization, Passive -- methods KW - Blood Component Removal -- instrumentation KW - Cell Separation -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78656291?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+apheresis&rft.atitle=Adoptive+immunotherapy%3A+novel+applications+of+blood+cell+separators.&rft.au=Klein%2C+H+G&rft.aulast=Klein&rft.aufirst=H&rft.date=1988-01-01&rft.volume=4&rft.issue=4&rft.spage=198&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+apheresis&rft.issn=07332459&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-14 N1 - Date created - 1989-03-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cholera toxin-induced ADP-ribosylation of a 46 kDa protein is decreased in brains of ethanol-fed mice. AN - 78654016; 3146919 AB - The acute in vitro effects of ethanol on cerebral cortical adenylate cyclase activity and beta-adrenergic receptor characteristics suggested a site of action of ethanol at Gs, the stimulatory guanine nucleotide binding protein. After chronic ethanol ingestion, the beta-adrenergic receptor appeared to be uncoupled (i.e., the form of the receptor with high affinity for agonist was undetectable), and stimulation of adenylate cyclase activity by isoproterenol or guanine nucleotides was reduced, suggesting an alteration in the properties of Gs. To further characterize this change, cholera and pertussis toxin-mediated 32P-ADP-ribosylation of mouse cortical membranes was assessed in mice that had chronically ingested ethanol in a liquid diet. 32P-labeled proteins were separated by SDS-PAGE and quantitated by autoradiography. There was a selective 30-50% decrease in cholera toxin-induced labeling of 46 kDa protein band in membranes of ethanol-fed mice, with no apparent change in pertussis toxin-induced labeling. The 46 kDa protein has a molecular weight similar to that of the alpha subunit of Gs, suggesting a reduced amount of this protein or a change in its characteristics as a substrate for cholera toxin-induced ADP-ribosylation in cortical membranes of ethanol-fed mice. JF - Advances in alcohol & substance abuse AU - Nhamburo, P T AU - Hoffman, P L AU - Tabakoff, B AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852. Y1 - 1988 PY - 1988 DA - 1988 SP - 103 EP - 105 VL - 7 IS - 3-4 SN - 0270-3106, 0270-3106 KW - Adenylate Cyclase Toxin KW - 0 KW - Phosphorus Radioisotopes KW - Virulence Factors, Bordetella KW - Ethanol KW - 3K9958V90M KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - Cholera Toxin KW - 9012-63-9 KW - Pertussis Toxin KW - EC 2.4.2.31 KW - GTP-Binding Proteins KW - EC 3.6.1.- KW - Index Medicus KW - Virulence Factors, Bordetella -- pharmacology KW - Animals KW - GTP-Binding Proteins -- metabolism KW - Mice, Inbred C57BL KW - Cholera Toxin -- pharmacology KW - Mice KW - Cerebral Cortex -- metabolism KW - Ethanol -- pharmacology KW - Adenosine Diphosphate -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78654016?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+alcohol+%26+substance+abuse&rft.atitle=Cholera+toxin-induced+ADP-ribosylation+of+a+46+kDa+protein+is+decreased+in+brains+of+ethanol-fed+mice.&rft.au=Nhamburo%2C+P+T%3BHoffman%2C+P+L%3BTabakoff%2C+B&rft.aulast=Nhamburo&rft.aufirst=P&rft.date=1988-01-01&rft.volume=7&rft.issue=3-4&rft.spage=103&rft.isbn=&rft.btitle=&rft.title=Advances+in+alcohol+%26+substance+abuse&rft.issn=02703106&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-17 N1 - Date created - 1989-03-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The acquired immunodeficiency syndrome and intravenous drug abuse. AN - 78642043; 3064841 AB - Intravenous drug abusers constitute 25 per cent of the cases of the acquired immunodeficiency syndrome (AIDS) in adults in the United States of America and 21 per cent of such cases in Europe. The potential for the rapid spread of the human immunodeficiency virus (HIV) among intravenous drug abusers exists because such drug abusers commonly share drug injection equipment. The heterosexual and perinatal spread of AIDS is also largely associated with intravenous drug abusers, and drug abusers have been identified as a major vector for the spread of the AIDS epidemic as it is associated with intravenous drug abuse. As long as intravenous drug abusers are addicted, they will continue to be at risk of contracting AIDS. Thus, the primary AIDS prevention strategy must be to help addicts to stop using drugs. It is suggested that drug abuse treatment resources should be expanded and outreach programmes developed to encourage more intravenous drug abusers to enter treatment. AIDS risk-reduction counseling must also be provided to intravenous drug abusers who continue injecting drugs, and to addicts and their sexual partners to prevent the sexual spread of HIV. Vigorous AIDS prevention initiatives must be undertaken now, using the most promising intervention strategies, while simultaneously evaluating and refining these strategies. JF - Bulletin on narcotics AU - Battjes, R J AU - Leukefeld, C G AU - Pickens, R W AU - Haverkos, H W AD - National Institute on Drug Abuse, Rockville, Maryland. Y1 - 1988 PY - 1988 DA - 1988 SP - 21 EP - 34 VL - 40 IS - 1 SN - 0007-523X, 0007-523X KW - Street Drugs KW - 0 KW - Index Medicus KW - AIDS/HIV KW - HIV Seropositivity KW - Risk Factors KW - Humans KW - Male KW - Female KW - Injections, Intravenous -- instrumentation KW - Acquired Immunodeficiency Syndrome -- prevention & control KW - Substance-Related Disorders -- therapy KW - Acquired Immunodeficiency Syndrome -- transmission UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78642043?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bulletin+on+narcotics&rft.atitle=The+acquired+immunodeficiency+syndrome+and+intravenous+drug+abuse.&rft.au=Battjes%2C+R+J%3BLeukefeld%2C+C+G%3BPickens%2C+R+W%3BHaverkos%2C+H+W&rft.aulast=Battjes&rft.aufirst=R&rft.date=1988-01-01&rft.volume=40&rft.issue=1&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Bulletin+on+narcotics&rft.issn=0007523X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-22 N1 - Date created - 1989-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - HLA antigen frequencies in HIV-1 seropositive disease-free individuals and patients with AIDS. AN - 78633131; 3216293 AB - HLA-A, -B, -C, -DR, and -DQ antigen phenotypes were determined in 266 Caucasian homosexual men, 90 of whom were HIV-1 seronegative, 94 HIV-1 seropositive AIDS-free, and 82 with a diagnosis of AIDS [36 with Kaposi's sarcoma (KS), 34 with opportunistic infection (OI), and 12 with KS and OI]. No significant differences in HLA-A or -B antigen frequencies were found in any comparisons of these groups. However, in comparisons of seropositive AIDS-free men with the AIDS groups, HLA-Cw7 was increased in frequency in OI and HLA-DR1, -DRw14, and -DQw1 in KS. HLA-DR3 and -DQw3 frequencies were decreased in KS, and DRw53 was decreased in OI. In a cohort of 102 HIV seropositive individuals that were followed for a mean of 43 months, AIDS developed in HLA-DR1 positive men more frequently than in individuals with other HLA-DR phenotypes (p = 0.02). These results demonstrate probable genetic differences between individuals developing KS and OI and indicate that the HLA-DR1 phenotype is a risk factor in disease progression in human immunodeficiency virus (HIV)-infected individuals. JF - Journal of acquired immune deficiency syndromes AU - Mann, D L AU - Murray, C AU - Yarchoan, R AU - Blattner, W A AU - Goedert, J J AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 13 EP - 17 VL - 1 IS - 1 SN - 0894-9255, 0894-9255 KW - HLA Antigens KW - 0 KW - HLA-A Antigens KW - HLA-B Antigens KW - HLA-C Antigens KW - HLA-DQ Antigens KW - HLA-DR Antigens KW - Index Medicus KW - AIDS/HIV KW - HLA-DR Antigens -- analysis KW - HLA-A Antigens -- analysis KW - HLA-C Antigens -- analysis KW - Humans KW - Opportunistic Infections -- immunology KW - HLA-B Antigens -- analysis KW - HLA-DQ Antigens -- analysis KW - Sarcoma, Kaposi -- immunology KW - Opportunistic Infections -- complications KW - Male KW - Sarcoma, Kaposi -- etiology KW - Acquired Immunodeficiency Syndrome -- complications KW - Acquired Immunodeficiency Syndrome -- immunology KW - HLA Antigens -- analysis KW - HIV Seropositivity -- immunology KW - HIV-1 UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78633131?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+acquired+immune+deficiency+syndromes&rft.atitle=HLA+antigen+frequencies+in+HIV-1+seropositive+disease-free+individuals+and+patients+with+AIDS.&rft.au=Mann%2C+D+L%3BMurray%2C+C%3BYarchoan%2C+R%3BBlattner%2C+W+A%3BGoedert%2C+J+J&rft.aulast=Mann&rft.aufirst=D&rft.date=1988-01-01&rft.volume=1&rft.issue=1&rft.spage=13&rft.isbn=&rft.btitle=&rft.title=Journal+of+acquired+immune+deficiency+syndromes&rft.issn=08949255&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-09 N1 - Date created - 1989-03-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Detection and characterization of the protein encoded by the chicken c-rel protooncogene. AN - 78632612; 2851122 AB - We have identified the protein encoded by chicken c-rel, the cellular homolog of the v-rel oncogene carried by reticuloendotheliosis virus. The protein has been detected in two avian lymphoid cell lines and in chick embryo fibroblasts. It has a molecular weight of 68,000, suggesting that the viral gene (which encodes a protein of 59,000 molecular weight) is a truncated form of its cellular homolog. The c-rel protein is found in the soluble cytoplasmic fraction of chicken lymphoid cells and is not associated in any stable way with other cellular proteins. Unlike the viral protein, p68c-rel is not detectably phosphorylated. JF - Oncogene research AU - Simek, S AU - Rice, N R AD - Laboratory of Molecular Virology and Carcinogenesis, BRI-Basic Research Program, NCI-Frederick Cancer Research Facility, MD 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 103 EP - 119 VL - 2 IS - 2 SN - 0890-6467, 0890-6467 KW - Oncogene Proteins v-rel KW - 0 KW - Proto-Oncogene Proteins KW - Proto-Oncogene Proteins c-rel KW - Retroviridae Proteins KW - Acetylglucosaminidase KW - EC 3.2.1.52 KW - Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase KW - EC 3.2.1.96 KW - Index Medicus KW - Animals KW - Chickens KW - Blotting, Northern KW - Phosphorylation KW - Peptide Mapping KW - Reticuloendotheliosis virus -- genetics KW - Genes, Viral KW - Cell Line KW - Fibroblasts KW - Retroviridae Proteins -- genetics KW - Retroviridae Proteins -- analysis KW - Proto-Oncogene Proteins -- analysis KW - Proto-Oncogenes KW - Proto-Oncogene Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78632612?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene+research&rft.atitle=Detection+and+characterization+of+the+protein+encoded+by+the+chicken+c-rel+protooncogene.&rft.au=Simek%2C+S%3BRice%2C+N+R&rft.aulast=Simek&rft.aufirst=S&rft.date=1988-01-01&rft.volume=2&rft.issue=2&rft.spage=103&rft.isbn=&rft.btitle=&rft.title=Oncogene+research&rft.issn=08906467&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-01 N1 - Date created - 1989-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Nitrite inhalants: patterns of abuse in Baltimore and Washington, D.C. AN - 78630455; 3265028 AB - Nitrite inhalants, as drugs of abuse, have received a new prominence in the literature since their use has been associated with Kaposi's Sarcoma and possibly other manifestations of acquired immunodeficiency syndrome (AIDS). Changes in patterns and prevalence of use have not been investigated since the onset of the AIDS epidemic. We have examined the abuse patterns of nitrite inhalants (poppers) in several different groups. The use of poppers among drug abusers in the Baltimore-Washington, D.C. metropolitan area has remained constant over the past 5 years, with the prevalence of use being approximately 11% for recreational drug users and 22% for heavy abusers. Self-reported use by a homosexual group had decreased over the same time period. Sixty-nine percent of the homosexual sample had experience with nitrities, but only 21% had used them in the 6 months prior to being surveyed. The mean interval since last use was 25 months, and since peak use, 4.1 years. Among substance abusers, nitrites appear to be a drug whose use starts late, with the mean age of first use being 25.6 years compared to 14.6 years for glue, 17.6 years for marijuana, and 18.5 years for heroin. We found both heterosexual and homosexual groups utilize nitrites primarily to "get high," but homosexuals more often use them during overt sexual activity. Experience with amyl nitrite was much more prevalent than that with the butyl derivative in both populations. We conclude that the prevalence of nitrite abuse among drug users has not changed as a result of the AIDS epidemic, but such use appears to have decreased within the homosexual community. JF - The American journal of drug and alcohol abuse AU - Lange, W R AU - Haertzen, C A AU - Hickey, J E AU - Snyder, F R AU - Dax, E M AU - Jaffe, J H AD - Addiction Research Center, National Institute on Drug Abuse, Baltimore, Maryland 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 29 EP - 39 VL - 14 IS - 1 SN - 0095-2990, 0095-2990 KW - Nitrites KW - 0 KW - Index Medicus KW - AIDS/HIV KW - District of Columbia KW - Cross-Sectional Studies KW - Humans KW - Adult KW - Acquired Immunodeficiency Syndrome -- psychology KW - Maryland KW - Homosexuality KW - Administration, Inhalation KW - Male KW - Female KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78630455?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+drug+and+alcohol+abuse&rft.atitle=Nitrite+inhalants%3A+patterns+of+abuse+in+Baltimore+and+Washington%2C+D.C.&rft.au=Lange%2C+W+R%3BHaertzen%2C+C+A%3BHickey%2C+J+E%3BSnyder%2C+F+R%3BDax%2C+E+M%3BJaffe%2C+J+H&rft.aulast=Lange&rft.aufirst=W&rft.date=1988-01-01&rft.volume=14&rft.issue=1&rft.spage=29&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+drug+and+alcohol+abuse&rft.issn=00952990&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-10 N1 - Date created - 1989-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Biochemical events associated with inhibition of B-cell proliferation by phorbol diesters. AN - 78630262; 3265131 AB - Phorbol myristate acelate (PMA), a potent tumor promoter, has a variety of effects on cells of the immune system resulting in altered patterns of cell proliferation and differentiation. Although PMA is mitogenic or co-mitogenic for human lymphocytes and murine T-cells, it inhibits proliferation of murine B-cells stimulated by LPS or anti-Ig. PMA, however, does not inhibit the ability of LPS or anti-Ig to activate B-cells, as evidenced by increased Ia antigen expression and RNA synthesis. In the present studies it was shown that inhibition of DNA synthesis by PMA coincided with qualitative and quantitative changes in phosphorylated proteins. In particular, PMA treatment resulted in a unique profile of phosphoproteins independent of LPS or anti-Ig treatment. Inhibition of DNA synthesis occurred over a wide range of PMA concentrations. At concentrations up to 10(-9) M, inhibition of proliferation correlated with decreased phosphatidylinositol turnover and decreased intracellular Ca2+ levels, suggesting that PMA affects the phosphoinositide signal transduction pathway. However, at PMA concentrations less than 10(-10) M, inhibition of anti-Ig- and LPS-mediated proliferation occurred without inhibition of the phosphoinositide transduction signal. At these concentrations, PMA-induced inhibition of DNA synthesis was highly sensitive to recombinant IL-2. These data suggest that the antiproliferative effects of PMA on B-cells stimulated by LPS or anti-Ig may be mediated by two mechanisms. At high concentrations, PMA causes a feedback regulation of the phosphoinositide-dependent messenger system, while at lower concentrations, PMA alters the response to specific growth factors. Since PMA induces unique phosphoproteins and both of these events can be regulated by protein phosphorylation, it is possible that these unique phosphoproteins are responsible for the antiproliferative effects of PMA. JF - International journal of immunopharmacology AU - Germolec, D R AU - Clark, G C AU - Blank, J A AU - Wiegand, G AU - Luster, M I AD - Systemic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 953 EP - 965 VL - 10 IS - 8 SN - 0192-0561, 0192-0561 KW - Antibodies, Anti-Idiotypic KW - 0 KW - Growth Substances KW - Lipopolysaccharides KW - Phosphatidylinositols KW - Phosphoproteins KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Calcium -- metabolism KW - Phosphatidylinositols -- metabolism KW - Animals KW - Lipopolysaccharides -- pharmacology KW - Growth Substances -- pharmacology KW - Antibodies, Anti-Idiotypic -- administration & dosage KW - In Vitro Techniques KW - Mice KW - Phosphoproteins -- metabolism KW - Lymphocyte Activation -- drug effects KW - B-Lymphocytes -- drug effects KW - Tetradecanoylphorbol Acetate -- pharmacology KW - B-Lymphocytes -- immunology KW - B-Lymphocytes -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78630262?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+immunopharmacology&rft.atitle=Biochemical+events+associated+with+inhibition+of+B-cell+proliferation+by+phorbol+diesters.&rft.au=Germolec%2C+D+R%3BClark%2C+G+C%3BBlank%2C+J+A%3BWiegand%2C+G%3BLuster%2C+M+I&rft.aulast=Germolec&rft.aufirst=D&rft.date=1988-01-01&rft.volume=10&rft.issue=8&rft.spage=953&rft.isbn=&rft.btitle=&rft.title=International+journal+of+immunopharmacology&rft.issn=01920561&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-03-06 N1 - Date created - 1989-03-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The activation of pyruvate dehydrogenase by glucagon in hepatocytes is diminished by phorbol myristate acetate: a role for cytoplasmic calcium in dehydrogenase regulation. AN - 78621387; 3213687 JF - Advances in experimental medicine and biology AU - Staddon, J M AU - Hansford, R G AD - National Institute on Aging, Gerontology Research Center, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 245 EP - 247 VL - 232 SN - 0065-2598, 0065-2598 KW - Pyruvate Dehydrogenase Complex KW - 0 KW - Glucagon KW - 9007-92-5 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Rats KW - Cytosol -- metabolism KW - Animals KW - Enzyme Activation KW - Cells, Cultured KW - Kinetics KW - Homeostasis KW - Liver -- enzymology KW - Glucagon -- pharmacology KW - Calcium -- physiology KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Pyruvate Dehydrogenase Complex -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78621387?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+experimental+medicine+and+biology&rft.atitle=The+activation+of+pyruvate+dehydrogenase+by+glucagon+in+hepatocytes+is+diminished+by+phorbol+myristate+acetate%3A+a+role+for+cytoplasmic+calcium+in+dehydrogenase+regulation.&rft.au=Staddon%2C+J+M%3BHansford%2C+R+G&rft.aulast=Staddon&rft.aufirst=J&rft.date=1988-01-01&rft.volume=232&rft.issue=&rft.spage=245&rft.isbn=&rft.btitle=&rft.title=Advances+in+experimental+medicine+and+biology&rft.issn=00652598&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-16 N1 - Date created - 1989-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of sodium azide on sea urchin embryos and gametes. AN - 78611075; 2905547 AB - Sodium azide (SA) was tested on sea urchin embryos and gametes (Paracentrotus lividus). Developing embryos were exposed to SA (10(-6) to 10(-3) M) up to pluteus larval stage, or for shorter intervals before or after hatching. Developmental defects in SA-exposed embryos consisted mainly of gut abnormalities, without any detectable differences between pre- or post-hatch-exposed embryos. SA-induced damage to gut was exerted during gastrulation, as evident by lectin binding of extracellular matrix. No mitotic damage was observed in SA-exposed embryos, nor could pH-related variations be detected in SA-induced embryotoxicity at pH's ranging from 8 to 6. Concurrently, no effect ensued in the exposure of unfertilized eggs to SA (10(-5) to 10(-2) M) both in terms of fertilization success and of offspring quality. When sperm were suspended in filtered seawater at pH's ranging from 8 to 6, and SA levels ranging from 10(-5) to 10(-2) M, fertilization success of SA-exposed sperm appeared to be modulated by pH, by displaying three distinct dose-response trends at pH 8, 7, or 6. The consequences of sperm pretreatment on offspring quality failed to show any significant SA-induced changes on larval malformations or mortality, while confirming the previously reported pH-induced increase of developmental defects in the offspring of acid-exposed sperm (Pagano et al.: Teratogenesis Carcinogen Mutagen 5:113-121, 1985). JF - Teratogenesis, carcinogenesis, and mutagenesis AU - Pagano, G AU - Cipollaro, M AU - Corsale, G AU - Esposito, A AU - Mineo, A AU - Ragucci, E AU - Giordano, G G AU - Kobayashi, N AU - Trieff, N M AD - National Cancer Institute, Fondazione G. Pascale, Naples, Italy. Y1 - 1988 PY - 1988 DA - 1988 SP - 363 EP - 376 VL - 8 IS - 6 SN - 0270-3211, 0270-3211 KW - Azides KW - 0 KW - Sodium Azide KW - 968JJ8C9DV KW - Index Medicus KW - Intestines -- embryology KW - Animals KW - Drug Administration Schedule KW - Dose-Response Relationship, Drug KW - Hydrogen-Ion Concentration KW - Mitosis -- drug effects KW - Fertilization -- drug effects KW - Male KW - Spermatozoa -- drug effects KW - Sea Urchins -- embryology KW - Azides -- toxicity KW - Ovum -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78611075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Teratogenesis%2C+carcinogenesis%2C+and+mutagenesis&rft.atitle=Effects+of+sodium+azide+on+sea+urchin+embryos+and+gametes.&rft.au=Pagano%2C+G%3BCipollaro%2C+M%3BCorsale%2C+G%3BEsposito%2C+A%3BMineo%2C+A%3BRagucci%2C+E%3BGiordano%2C+G+G%3BKobayashi%2C+N%3BTrieff%2C+N+M&rft.aulast=Pagano&rft.aufirst=G&rft.date=1988-01-01&rft.volume=8&rft.issue=6&rft.spage=363&rft.isbn=&rft.btitle=&rft.title=Teratogenesis%2C+carcinogenesis%2C+and+mutagenesis&rft.issn=02703211&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-16 N1 - Date created - 1989-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alterations in craniofacial growth induced by isotretinoin (13-cis-retinoic acid) in mouse whole embryo and primary mesenchymal cell culture. AN - 78606190; 3209676 AB - Recent evidence has demonstrated that 13-cis-retinoic acid (13-cis-RA, or isotretinoin) is responsible for various craniofacial malformations in the rodent and human embryo. Our studies have been directed toward understanding this effect using mouse whole embryo and primary cell cultures. In whole embryo culture, 13-cis-RA caused significant overall embryonic growth retardation, especially in the primary and secondary palatal processes. In embryos explanted on day 10 of gestation and exposed for 24 or 48 hr, the mesenchyme beneath the epithelium of the nasal and maxillary processes contained pyknotic nuclei as well as a dramatically reduced number of nuclei incorporating 3H-thymidine. The secondary palatal processes and the roof of the oral-nasal cavity had fewer mesenchymal cells than control embryos. The incorporation of 3H-thymidine into TCA-insoluble macromolecules was 30% less in the retinoid-treated heads. In primary cell cultures from day-12 mouse secondary palatal mesenchyme, subsequent cell growth was decreased at concentrations of 13-cis-RA greater than 1 X 10(-5) M. After a 40-hr treatment period, labeling indices in retinoid-treated cells were significantly lower than control values (25% compared with 40%). Retinoic acid also caused a significant, concentration-dependent decrease in 3H-thymidine incorporation. The inhibitory effect of 13-cis-RA on proliferation of oral-nasal mesenchymal cells appears to be related to the production of craniofacial malformations. JF - Journal of craniofacial genetics and developmental biology AU - Watanabe, T AU - Goulding, E H AU - Pratt, R M AD - Experimental Teratogenesis Section, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 21 EP - 33 VL - 8 IS - 1 SN - 0270-4145, 0270-4145 KW - Teratogens KW - 0 KW - Isotretinoin KW - EH28UP18IF KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Reference Values KW - Cells, Cultured KW - Mice KW - Organ Culture Techniques KW - Female KW - DNA Replication -- drug effects KW - Pregnancy KW - Skull -- embryology KW - Palate -- drug effects KW - Facial Bones -- embryology KW - Facial Bones -- abnormalities KW - Skull -- abnormalities KW - Facial Bones -- drug effects KW - Skull -- drug effects KW - Isotretinoin -- toxicity KW - Embryo, Mammalian -- drug effects KW - Palate -- embryology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78606190?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+craniofacial+genetics+and+developmental+biology&rft.atitle=Alterations+in+craniofacial+growth+induced+by+isotretinoin+%2813-cis-retinoic+acid%29+in+mouse+whole+embryo+and+primary+mesenchymal+cell+culture.&rft.au=Watanabe%2C+T%3BGoulding%2C+E+H%3BPratt%2C+R+M&rft.aulast=Watanabe&rft.aufirst=T&rft.date=1988-01-01&rft.volume=8&rft.issue=1&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Journal+of+craniofacial+genetics+and+developmental+biology&rft.issn=02704145&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-23 N1 - Date created - 1989-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Novel phosphorylation of c-ras p21 by protein kinases. AN - 78605961; 2849744 AB - Novel p21 phosphorylation was found in cells expressing high levels of this product of c-H- and c-K-ras genes. Phorbol 12,13-dibutyrate, a protein kinase C (PKC) activator, and permeable c-AMP derivatives, which activate protein kinase A (PKA), stimulated phosphorylation of K-ras(4B) p21 in 416B cells 3 to 5 fold. By tryptic peptide mapping, it was found that both PKC and PKA phosphorylated in vitro the K-ras p21 at the same site as was found in p21 from cells labeled with [32P]orthophosphate in vivo. A common site of H-ras p21 was also phosphorylated by both PKC and PKA, although phosphopeptides of H-ras p21 were distinct from those of K-ras p21. The construction of a mutant by site-directed mutagenesis allowed the identification of serine-177 as the phosphorylation site of H-ras p21. This novel phosphorylation site lies in the hypervariable region, which links the globular catalytic domain of p21 to the membrane-anchoring site at the C-terminus, a location suggesting that this phosphorylation plays a role in modulating transmembrane signaling. JF - Oncogene research AU - Saikumar, P AU - Ulsh, L S AU - Clanton, D J AU - Huang, K P AU - Shih, T Y AD - Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 213 EP - 222 VL - 3 IS - 3 SN - 0890-6467, 0890-6467 KW - Protein Kinase Inhibitors KW - 0 KW - Proto-Oncogene Proteins KW - Recombinant Fusion Proteins KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Cyclic AMP KW - E0399OZS9N KW - Protein Kinases KW - EC 2.7.- KW - Protein-Serine-Threonine Kinases KW - EC 2.7.11.1 KW - Proto-Oncogene Proteins p21(ras) KW - EC 3.6.5.2 KW - Index Medicus KW - Animals KW - Peptide Mapping KW - Protein Processing, Post-Translational KW - Amino Acid Sequence KW - Phorbol 12,13-Dibutyrate -- pharmacology KW - Recombinant Fusion Proteins -- metabolism KW - Rats KW - Phosphorylation KW - Cyclic AMP -- metabolism KW - Molecular Sequence Data KW - Protein Conformation KW - Protein Kinases -- metabolism KW - Proto-Oncogene Proteins -- metabolism KW - Proto-Oncogene Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78605961?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene+research&rft.atitle=Novel+phosphorylation+of+c-ras+p21+by+protein+kinases.&rft.au=Saikumar%2C+P%3BUlsh%2C+L+S%3BClanton%2C+D+J%3BHuang%2C+K+P%3BShih%2C+T+Y&rft.aulast=Saikumar&rft.aufirst=P&rft.date=1988-01-01&rft.volume=3&rft.issue=3&rft.spage=213&rft.isbn=&rft.btitle=&rft.title=Oncogene+research&rft.issn=08906467&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-06 N1 - Date created - 1989-02-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Inhalant overview. AN - 78602239; 3145433 JF - NIDA research monograph AU - Crider, R A AU - Rouse, B A AD - Division of Epidemiology and Statistical Analysis, National Institute on Drug Abuse. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 7 VL - 85 SN - 1046-9516, 1046-9516 KW - Aerosols KW - 0 KW - Solvents KW - Index Medicus KW - Humans KW - Administration, Inhalation KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78602239?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Inhalant+overview.&rft.au=Crider%2C+R+A%3BRouse%2C+B+A&rft.aulast=Crider&rft.aufirst=R&rft.date=1988-01-01&rft.volume=85&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-22 N1 - Date created - 1989-02-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Treatment services for adolescent drug abusers: introduction and overview. AN - 78601873; 3145423 JF - NIDA research monograph AU - Rahdert, E R AD - Division of Clinical Research, National Institute on Drug Abuse, Rockville, Maryland 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 3 VL - 77 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - Humans KW - Michigan KW - Adolescent KW - Male KW - Female KW - Substance-Related Disorders -- therapy KW - Health Services -- economics KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78601873?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Treatment+services+for+adolescent+drug+abusers%3A+introduction+and+overview.&rft.au=Rahdert%2C+E+R&rft.aulast=Rahdert&rft.aufirst=E&rft.date=1988-01-01&rft.volume=77&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-13 N1 - Date created - 1989-02-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Mortality among agricultural extension agents. AN - 78597132; 3207102 AB - The mortality experience of agricultural extension agents in the Cooperative Extension Service (CES) of the U.S. Department of Agriculture who died during the period January 1, 1970-December 31, 1979 (n = 1,495 white males) was evaluated in proportionate-mortality and case-control studies. The proportionate-mortality analysis was used to identify cancers that might be elevated in this occupational group compared with the U.S. white male population. All cancers with a significantly elevated proportionate-mortality ratio were more thoroughly evaluated in the case-control study, where there is presumably less of a selection bias in the comparison. In the case-control study, leukemia demonstrated a statistically significant linear trend with duration of employment as an extension agent. Smaller, but nonsignificant, trends were seen for non-Hodgkin's lymphoma, multiple myeloma, and brain cancer. The odds ratio for Hodgkin's disease and cancers of the colon, prostate, and kidney did not vary with the number of years on the job. These patterns resemble cancer risks seen among farmers, suggesting that agricultural factors may also play a role in the origin of these tumors among extension agents. JF - American journal of industrial medicine AU - Alavanja, M C AU - Blair, A AU - Merkle, S AU - Teske, J AU - Eaton, B AD - Division of Cancer Etiology, National Cancer Institute, Bethesda, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 167 EP - 176 VL - 14 IS - 2 SN - 0271-3586, 0271-3586 KW - Index Medicus KW - United States KW - Leukemia -- mortality KW - Neoplasms -- mortality KW - Aged, 80 and over KW - Risk Factors KW - Humans KW - Adult KW - Environmental Exposure KW - Aged KW - Middle Aged KW - Male KW - Agricultural Workers' Diseases -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78597132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Mortality+among+agricultural+extension+agents.&rft.au=Alavanja%2C+M+C%3BBlair%2C+A%3BMerkle%2C+S%3BTeske%2C+J%3BEaton%2C+B&rft.aulast=Alavanja&rft.aufirst=M&rft.date=1988-01-01&rft.volume=14&rft.issue=2&rft.spage=167&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-02-09 N1 - Date created - 1989-02-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Amino acid sequence and physiological characterization of toxins from the venom of the scorpion Centruroides limpidus tecomanus Hoffmann. AN - 78581826; 2849217 AB - The complete amino acid sequence of the major toxic component (II.20.3.4), named toxin 1, from the venom of the Mexican scorpion C. l. tecomanus is reported. The sequence (66 amino acids) was obtained by direct Edman degradation of reduced and alkylated toxin, followed by sequence determination of selected peptides separated after enzymatic cleavage with S. aureus V8 protease. In cultured chick dorsal root ganglion cells, 0.5 microM toxin 1 slowed down specifically the time course of Na+ current inactivation, while Ca2+ currents from the same preparation were little affected. In neonatal rat ventricular heart cells, toxin 1, at concentrations between 0.1 and 0.5 microM, reduced Na+ currents without changing the kinetics and Ca2+ currents were unaffected. Comparative analysis of the primary structure of this toxin with other scorpion toxins shows a high degree of similarity with the north American scorpion toxins. This analysis suggests that the 'fine tuning' of the molecular mechanism of action of these toxins is related to variations in the primary structure as well as to the type of membrane under study (tissue specificity). JF - Toxicon : official journal of the International Society on Toxinology AU - Martin, B M AU - Carbone, E AU - Yatani, A AU - Brown, A M AU - Ramírez, A N AU - Gurrola, G B AU - Possani, L D AD - National Institute of Mental Health, Molecular Neurogenetics Unit, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 785 EP - 794 VL - 26 IS - 9 SN - 0041-0101, 0041-0101 KW - Scorpion Venoms KW - 0 KW - Sodium Channels KW - Index Medicus KW - Rats KW - Animals KW - Chick Embryo KW - Molecular Sequence Data KW - Heart -- drug effects KW - Amino Acid Sequence KW - Action Potentials -- drug effects KW - Ganglia, Spinal -- drug effects KW - Sodium Channels -- drug effects KW - Scorpion Venoms -- analysis KW - Scorpion Venoms -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78581826?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicon+%3A+official+journal+of+the+International+Society+on+Toxinology&rft.atitle=Amino+acid+sequence+and+physiological+characterization+of+toxins+from+the+venom+of+the+scorpion+Centruroides+limpidus+tecomanus+Hoffmann.&rft.au=Martin%2C+B+M%3BCarbone%2C+E%3BYatani%2C+A%3BBrown%2C+A+M%3BRam%C3%ADrez%2C+A+N%3BGurrola%2C+G+B%3BPossani%2C+L+D&rft.aulast=Martin&rft.aufirst=B&rft.date=1988-01-01&rft.volume=26&rft.issue=9&rft.spage=785&rft.isbn=&rft.btitle=&rft.title=Toxicon+%3A+official+journal+of+the+International+Society+on+Toxinology&rft.issn=00410101&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-26 N1 - Date created - 1989-01-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cocaine-induced behavioral sensitization and kindling: implications for the emergence of psychopathology and seizures. AN - 78581731; 2904782 JF - Annals of the New York Academy of Sciences AU - Post, R M AU - Weiss, S R AU - Pert, A AD - Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 292 EP - 308 VL - 537 SN - 0077-8923, 0077-8923 KW - Antipsychotic Agents KW - 0 KW - Amphetamine KW - CK833KGX7E KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Drug Tolerance KW - Animals KW - Nucleus Accumbens -- drug effects KW - Nucleus Accumbens -- physiology KW - Antipsychotic Agents -- pharmacology KW - Conditioning (Psychology) -- physiology KW - Caudate Nucleus -- drug effects KW - Amygdala -- physiology KW - Amphetamine -- pharmacology KW - Caudate Nucleus -- physiology KW - Seizures -- chemically induced KW - Behavior, Animal -- drug effects KW - Kindling, Neurologic -- drug effects KW - Mental Disorders -- chemically induced KW - Cocaine -- toxicity KW - Cocaine -- pharmacology KW - Cocaine -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78581731?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Cocaine-induced+behavioral+sensitization+and+kindling%3A+implications+for+the+emergence+of+psychopathology+and+seizures.&rft.au=Post%2C+R+M%3BWeiss%2C+S+R%3BPert%2C+A&rft.aulast=Post&rft.aufirst=R&rft.date=1988-01-01&rft.volume=537&rft.issue=&rft.spage=292&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-23 N1 - Date created - 1989-01-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Intradentate colchicine disrupts the acquisition and performance of a working memory task in the radial arm maze. AN - 78578600; 3200514 AB - Rats were given bilateral injections of colchicine into the dorsal and ventral hippocampus to study the role of the dentate gyrus granule cells in the acquisition and performance of a spatial, working memory task in the radial arm maze. Three weeks after intradentate injections, rats were trained in a task in which all eight arms were baited prior to each daily trail. For up to 20 days of training, colchicine-treated rats were significantly impaired in the performance of the task. In another study, rats received 20 days of training and then were given intradentate colchicine. Three weeks later, the performance of the colchicine-treated rats was impaired for up to 20 days of testing. A third experiment tested the ability of colchicine-treated rats to learn a task in which the same four arms of the maze were baited, while the remaining arms were never baited. Colchicine-treated rats were significantly impaired in their ability to perform this version of the task. Histological verification indicated that colchicine resulted in a relatively select loss of granule cells, while sparing pyramidal cells in the hippocampus. These data suggest that the hippocampus plays an integral role in the performance of the place tasks used in these experiments. JF - Neurotoxicology AU - McLamb, R L AU - Mundy, W R AU - Tilson, H A AD - Laboratory of Molecular and Integrative Neuroscience, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 521 EP - 528 VL - 9 IS - 3 SN - 0161-813X, 0161-813X KW - Colchicine KW - SML2Y3J35T KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Retention (Psychology) -- drug effects KW - Hippocampus -- pathology KW - Male KW - Hippocampus -- drug effects KW - Colchicine -- toxicity KW - Memory -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78578600?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurotoxicology&rft.atitle=Intradentate+colchicine+disrupts+the+acquisition+and+performance+of+a+working+memory+task+in+the+radial+arm+maze.&rft.au=McLamb%2C+R+L%3BMundy%2C+W+R%3BTilson%2C+H+A&rft.aulast=McLamb&rft.aufirst=R&rft.date=1988-01-01&rft.volume=9&rft.issue=3&rft.spage=521&rft.isbn=&rft.btitle=&rft.title=Neurotoxicology&rft.issn=0161813X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-19 N1 - Date created - 1989-01-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The therapeutic latency of neuroleptic drugs and nonspecific postjunctional supersensitivity. AN - 78574575; 2904695 AB - It is suggested that the antidopaminergic effects of neuroleptics are not directly responsible for the antipsychotic effect but, rather, that the antipsychotic effect is related to secondary changes in the efficacy of transmission at corticostriatal excitatory synapses. Arguments are presented in support of the following: (1) acute dopaminergic antagonism produces a relatively nonspecific sedation or deactivation, but most of the amelioration of psychosis develops slowly; (2) the development of dopaminergic supersensitivity is responsible for tolerance to the sedative effects of neuroleptics; and (3) excitatory synapses of the corticostriatal pathway, mediated by glutamic acid, are located on the same dendritic spines as the striatal dopaminergic synapses. Concomitant with the development of dopaminergic supersensitivity, these glutamate synapses become subsensitive. The glutamatergic subsensitivity is a result of the nonspecific nature of postsynaptic denervation supersensitivity. It is suggested that subsensitivity of striatal glutamate-mediated synapses is directly responsible for the antipsychotic effect of neuroleptic drugs. In support of this hypothesis, chronic neuroleptic administration was found to decrease the behavioral responsivity of mice to the glutamate agonist, quisqualic acid, and to the the antagonist, glutamic acid diethyl ester. JF - Schizophrenia bulletin AU - Freed, W J AD - Neuropsychiatry Branch, NIMH, Neuropsychiatric Research Hospital at Saint Elizabeths, Washington, DC 20032. Y1 - 1988 PY - 1988 DA - 1988 SP - 269 EP - 277 VL - 14 IS - 2 SN - 0586-7614, 0586-7614 KW - Antipsychotic Agents KW - 0 KW - Receptors, Dopamine KW - Receptors, Glutamate KW - Receptors, Neurotransmitter KW - Index Medicus KW - Animals KW - Humans KW - Receptors, Neurotransmitter -- drug effects KW - Mice KW - Receptors, Dopamine -- drug effects KW - Brain -- drug effects KW - Antipsychotic Agents -- therapeutic use KW - Schizophrenic Psychology KW - Schizophrenia -- drug therapy KW - Antipsychotic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78574575?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Schizophrenia+bulletin&rft.atitle=The+therapeutic+latency+of+neuroleptic+drugs+and+nonspecific+postjunctional+supersensitivity.&rft.au=Freed%2C+W+J&rft.aulast=Freed&rft.aufirst=W&rft.date=1988-01-01&rft.volume=14&rft.issue=2&rft.spage=269&rft.isbn=&rft.btitle=&rft.title=Schizophrenia+bulletin&rft.issn=05867614&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-19 N1 - Date created - 1989-01-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Behavioral impairment in the rat after colchicine lesions of the hippocampus and nucleus basalis. AN - 78574463; 3200513 AB - Rats were given bilateral injections of colchicine into the nucleus basalis (NBM; 1.0 or 2.0 micrograms/site), hippocampus (HPC; 1.25 or 2.5 micrograms/site), or both areas (COM; 1.0 NBM, 1.25 HPC) and examined for changes in locomotor activity, passive avoidance behavior, and spatial navigation in a water maze task. Colchicine injected into the HPC caused a dose-related increase in locomotor activity 7 days after treatment which declined with repeated testing. Motor activity in NBM-lesioned rats was not significantly different from control. Rats with the COM lesion were more active than controls 7 days after treatment and remained hyperactive over the 3 week testing period. Retention of a step-through passive avoidance task was examined 18 days after surgery. HPC lesions had no apparent effect on passive avoidance behavior. NBM lesions causes a dose-dependent decrease in step-through latencies, while latencies in the combined group were comparable to the low dose NBM group. In the spatial navigation task, HPC and COM lesions impaired acquisition, with little indication of learning in the combined group. NBM lesions had no effect in the water maze. These data suggest that combined lesions of the NBM and HPC cause lasting behavioral impairments and may be useful as a model for neurodegenerative disorders such as Alzheimer's disease. JF - Neurotoxicology AU - Mundy, W R AU - Tilson, H A AD - Laboratory of Behavioral and Neurological Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 511 EP - 519 VL - 9 IS - 3 SN - 0161-813X, 0161-813X KW - Colchicine KW - SML2Y3J35T KW - Index Medicus KW - Rats KW - Animals KW - Swimming KW - Rats, Inbred F344 KW - Motor Activity -- drug effects KW - Avoidance Learning -- drug effects KW - Male KW - Colchicine -- toxicity KW - Behavior, Animal -- drug effects KW - Basal Ganglia -- drug effects KW - Hippocampus -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78574463?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurotoxicology&rft.atitle=Behavioral+impairment+in+the+rat+after+colchicine+lesions+of+the+hippocampus+and+nucleus+basalis.&rft.au=Mundy%2C+W+R%3BTilson%2C+H+A&rft.aulast=Mundy&rft.aufirst=W&rft.date=1988-01-01&rft.volume=9&rft.issue=3&rft.spage=511&rft.isbn=&rft.btitle=&rft.title=Neurotoxicology&rft.issn=0161813X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-19 N1 - Date created - 1989-01-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Fatty acylation of proteins. AN - 78569273; 3058168 JF - Annual review of cell biology AU - Schultz, A M AU - Henderson, L E AU - Oroszlan, S AD - Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research Facility, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 611 EP - 647 VL - 4 SN - 0743-4634, 0743-4634 KW - Amides KW - 0 KW - Esters KW - Fatty Acids KW - Myelin Proteins KW - Phosphatidylinositols KW - Proteins KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Animals KW - Solubility KW - Myelin Proteins -- physiology KW - Acylation KW - Structure-Activity Relationship KW - Protein Processing, Post-Translational KW - Proteins -- metabolism KW - Fatty Acids -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78569273?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annual+review+of+cell+biology&rft.atitle=Fatty+acylation+of+proteins.&rft.au=Schultz%2C+A+M%3BHenderson%2C+L+E%3BOroszlan%2C+S&rft.aulast=Schultz&rft.aufirst=A&rft.date=1988-01-01&rft.volume=4&rft.issue=&rft.spage=611&rft.isbn=&rft.btitle=&rft.title=Annual+review+of+cell+biology&rft.issn=07434634&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-18 N1 - Date created - 1989-01-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Dosimeters of human exposure to carcinogens: polycyclic aromatic hydrocarbon-macromolecular adducts. AN - 78568164; 3143667 AB - The metabolic activation of polycyclic aromatic hydrocarbons (PAH), for example benzo[a]pyrene, leads to the formation of carcinogen-macromolecular adducts. Methods that make it possible to detect low levels of these adducts in human peripheral blood samples should be useful in the dosimetry of human exposure to carcinogens. We demonstrated previously the usefulness of enzyme immunoassays and of synchronous fluorescence spectroscopy (SFS) for detecting and characterizing low levels of PAH-macromolecular adducts present in synthetic adduct mixtures. These methods have now been refined and applied to the analysis of samples of peripheral blood collected from occupationally exposed individuals (coke-oven workers) and from people attending smoking cessation clinics. The results of both immunoassays and SFS show the presence of benzo[a]pyrene diol epoxide (BPDE)-DNA, BPDE-haemoglobin and other putative PAH-macromolecular adducts in peripheral blood samples from certain individuals. JF - IARC scientific publications AU - Weston, A AU - Willey, J C AU - Manchester, D K AU - Wilson, V L AU - Brooks, B R AU - Choi, J S AU - Poirier, M C AU - Trivers, G E AU - Newman, M J AU - Mann, D L AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 181 EP - 189 IS - 89 SN - 0300-5038, 0300-5038 KW - Carcinogens, Environmental KW - 0 KW - DNA Adducts KW - Hemoglobins KW - Phosphorus Radioisotopes KW - Polycyclic Compounds KW - benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA KW - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide KW - 55097-80-8 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide -- immunology KW - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide -- analysis KW - Spectrometry, Fluorescence KW - Hemoglobins -- metabolism KW - Humans KW - Immunoassay KW - DNA -- metabolism KW - DNA -- analysis KW - Carcinogens, Environmental -- metabolism KW - DNA -- immunology KW - Polycyclic Compounds -- metabolism KW - Environmental Monitoring -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78568164?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=IARC+scientific+publications&rft.atitle=Dosimeters+of+human+exposure+to+carcinogens%3A+polycyclic+aromatic+hydrocarbon-macromolecular+adducts.&rft.au=Weston%2C+A%3BWilley%2C+J+C%3BManchester%2C+D+K%3BWilson%2C+V+L%3BBrooks%2C+B+R%3BChoi%2C+J+S%3BPoirier%2C+M+C%3BTrivers%2C+G+E%3BNewman%2C+M+J%3BMann%2C+D+L&rft.aulast=Weston&rft.aufirst=A&rft.date=1988-01-01&rft.volume=&rft.issue=89&rft.spage=181&rft.isbn=&rft.btitle=&rft.title=IARC+scientific+publications&rft.issn=03005038&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-18 N1 - Date created - 1989-01-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Role of oncogenes in chemical carcinogenesis: extrapolation from rodents to humans. AN - 78567683; 3058603 AB - Proto-oncogenes are cellular genes that are expressed during normal growth and development processes. These genes can be activated to cancer-causing oncogenes by point mutations or by gross DNA rearrangement, such as chromosomal translocation or gene amplification. Activated versions of proto-oncogenes have been observed in various human and rodent tumours. Examples will be discussed, as will the activation of proto-oncogenes by chemical carcinogens. Most chemicals are classified as potentially hazardous to humans on the basis of long-term carcinogenesis studies in rodents. Oncogene analysis of tumours of spontaneous origin and from carcinogenesis studies may aid in risk analyses on the basis of rodent carcinogenesis studies. JF - IARC scientific publications AU - Anderson, M W AU - Maronpot, R R AU - Reynolds, S H AD - Laboratory of Biochemical Risk Analysis, National Institute of Environmental Health Sciences, Research Triangle Park, NC. Y1 - 1988 PY - 1988 DA - 1988 SP - 477 EP - 485 IS - 89 SN - 0300-5038, 0300-5038 KW - Carcinogens KW - 0 KW - Index Medicus KW - Rats KW - Carcinogens -- pharmacology KW - Animals KW - Transfection KW - Humans KW - Mice KW - Translocation, Genetic KW - Gene Amplification KW - Neoplasms -- chemically induced KW - Proto-Oncogenes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78567683?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=IARC+scientific+publications&rft.atitle=Role+of+oncogenes+in+chemical+carcinogenesis%3A+extrapolation+from+rodents+to+humans.&rft.au=Anderson%2C+M+W%3BMaronpot%2C+R+R%3BReynolds%2C+S+H&rft.aulast=Anderson&rft.aufirst=M&rft.date=1988-01-01&rft.volume=&rft.issue=89&rft.spage=477&rft.isbn=&rft.btitle=&rft.title=IARC+scientific+publications&rft.issn=03005038&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-18 N1 - Date created - 1989-01-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Behavioral symptomatology in dementia of the Alzheimer type. AN - 78567455; 3196470 JF - Alzheimer disease and associated disorders AU - Kumar, A AU - Koss, E AU - Metzler, D AU - Moore, A AU - Friedland, R P AD - Laboratory of Neurosciences, National Institute on Aging, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 363 EP - 365 VL - 2 IS - 4 SN - 0893-0341, 0893-0341 KW - Index Medicus KW - Humans KW - Mental Disorders -- diagnosis KW - Depression -- etiology KW - Alzheimer Disease -- complications KW - Mental Disorders -- etiology KW - Depression -- diagnosis KW - Paranoid Disorders -- etiology KW - Paranoid Disorders -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78567455?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alzheimer+disease+and+associated+disorders&rft.atitle=Behavioral+symptomatology+in+dementia+of+the+Alzheimer+type.&rft.au=Kumar%2C+A%3BKoss%2C+E%3BMetzler%2C+D%3BMoore%2C+A%3BFriedland%2C+R+P&rft.aulast=Kumar&rft.aufirst=A&rft.date=1988-01-01&rft.volume=2&rft.issue=4&rft.spage=363&rft.isbn=&rft.btitle=&rft.title=Alzheimer+disease+and+associated+disorders&rft.issn=08930341&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-26 N1 - Date created - 1989-01-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - DNA restriction fragment length polymorphism analysis of human bronchogenic carcinoma. AN - 78565196; 2904414 AB - DNA restriction fragment length polymorphism (RFLP) analysis has been successfully applied both to evaluate possible human susceptibility factors as well as to identify genes involved in malignant transformation of human cells. In this report we review previous applications of RFLP analysis to evaluation of human malignancies and discuss our RFLP studies of human bronchogenic carcinoma, which are in progress. Preliminary results of our analysis of the RFLP associated with the cHa ras variable tandem repeat (VTR) indicate that rare alleles of this VTR are more frequent in patients with bronchogenic carcinoma than in controls. JF - IARC scientific publications AU - Willey, J C AU - Weston, A AU - Haugen, A AU - Krontiris, T AU - Resau, J AU - McDowell, E AU - Trump, B AU - Harris, C C AD - Laboratory of Human Carcinogenesis, National Institutes of Health, Bethesda, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 439 EP - 450 IS - 89 SN - 0300-5038, 0300-5038 KW - Index Medicus KW - Genes KW - Humans KW - Mutation KW - Carcinoma, Bronchogenic -- etiology KW - Carcinoma, Bronchogenic -- genetics KW - Polymorphism, Restriction Fragment Length KW - Polymorphism, Genetic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78565196?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=IARC+scientific+publications&rft.atitle=DNA+restriction+fragment+length+polymorphism+analysis+of+human+bronchogenic+carcinoma.&rft.au=Willey%2C+J+C%3BWeston%2C+A%3BHaugen%2C+A%3BKrontiris%2C+T%3BResau%2C+J%3BMcDowell%2C+E%3BTrump%2C+B%3BHarris%2C+C+C&rft.aulast=Willey&rft.aufirst=J&rft.date=1988-01-01&rft.volume=&rft.issue=89&rft.spage=439&rft.isbn=&rft.btitle=&rft.title=IARC+scientific+publications&rft.issn=03005038&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-18 N1 - Date created - 1989-01-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Uterine receptivity for blastocyst implantation. AN - 78563476; 3057996 AB - The receptivity for blastocyst implantation is controlled by progesterone and in some species by the synergistic action of progesterone and estrogen. The duration of the receptive phase, the so-called "window," is short in rodents (less than 24 hours) and may be three days in the primate. Once the uterus becomes receptive, it automatically becomes refractory at the end of the receptive phase. The uterus in the refractory phase can be toxic to the blastocyst in small laboratory animals. The endometrium of the receptive uterus may be characterized by the following parameters: (1) Formation of bulbous protrusions on the apical surface of the luminal epithelium; (2) Secretion of the stage-specific glycoproteins by the luminal epithelium; (3) Readiness of stromal cells to decidualize when appropriate stimulation is applied; and (4) Reorganization and changes of stromal extracellular matrix components so that stromal cells are conditioned for decidualization, and after decidualization the appearance of basement membrane components in the matrix. JF - Annals of the New York Academy of Sciences AU - Yoshinaga, K AD - Reproductive Sciences Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 424 EP - 431 VL - 541 SN - 0077-8923, 0077-8923 KW - Index Medicus KW - Embryonic and Fetal Development KW - Humans KW - Embryo Transfer KW - Female KW - Pregnancy KW - Embryo Implantation KW - Uterus -- physiology KW - Blastocyst -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78563476?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Uterine+receptivity+for+blastocyst+implantation.&rft.au=Yoshinaga%2C+K&rft.aulast=Yoshinaga&rft.aufirst=K&rft.date=1988-01-01&rft.volume=541&rft.issue=&rft.spage=424&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-06 N1 - Date created - 1989-01-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Radon-222 concentration in groundwater and cancer mortality in North Carolina. AN - 78563083; 3198278 AB - In a geographic correlation study, we explored the possibility that residential exposure to radon in groundwater may be related to cancers other than lung cancer. Measurements of radon in groundwater and 1978-1982 cancer mortality data from North Carolina, USA were used to investigate this relationship. Counties were categorized in two levels of radon exposure according to measured radon concentration and geology. In the lower exposure group (unexposed) county mean radon concentrations ranged from 0-228 pCi/l (0-8436 Bq/m3), and in the upper group (potentially exposed) the range of county average concentrations was 229-10892 pCi/l (8473-403004 Bq/m3) (median 1375 pCi/l (50875 Bq/m3)). Adjusted mortality ratios and 95% confidence intervals were calculated for selected cancers, including leukemias, gastro-intestinal tract cancers, and respiratory tract cancers excluding lung cancer. In contrast to other ecologic studies, we found no consistent association between radon level and cancer mortality. JF - International archives of occupational and environmental health AU - Collman, G W AU - Loomis, D P AU - Sandler, D P AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 13 EP - 18 VL - 61 IS - 1-2 SN - 0340-0131, 0340-0131 KW - Water Pollutants KW - 0 KW - Water Pollutants, Radioactive KW - Radon KW - Q74S4N8N1G KW - Index Medicus KW - Sex Factors KW - Risk Factors KW - Humans KW - North Carolina KW - Male KW - Female KW - Water Pollutants -- analysis KW - Neoplasms -- mortality KW - Radon -- analysis KW - Water Pollutants, Radioactive -- analysis KW - Neoplasms, Radiation-Induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78563083?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+archives+of+occupational+and+environmental+health&rft.atitle=Radon-222+concentration+in+groundwater+and+cancer+mortality+in+North+Carolina.&rft.au=Collman%2C+G+W%3BLoomis%2C+D+P%3BSandler%2C+D+P&rft.aulast=Collman&rft.aufirst=G&rft.date=1988-01-01&rft.volume=61&rft.issue=1-2&rft.spage=13&rft.isbn=&rft.btitle=&rft.title=International+archives+of+occupational+and+environmental+health&rft.issn=03400131&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1989-01-25 N1 - Date created - 1989-01-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Magnetic resonance imaging (MRI): a new tool in experimental toxicologic pathology. AN - 78542251; 3194661 AB - Magnetic Resonance Imaging (MRI) is a noninvasive imaging technique that provides multidimensional images of the soft tissues of the body. This imaging technique has proven to be an excellent diagnostic and experimental tool for the detection of pathologic alterations in soft tissues, as well as an adjunct screening method for following the genesis, progression, or regression of chemically induced lesions in the same live animal. Future applications of MRI technology in small animals include MRI microscopy, mapping of vascular or circulatory alterations, measurement of perfusion and diffusion rates of body fluids, and acquisition of cell metabolic states in combination with Nuclear Magnetic Resonance (NMR) spectroscopy, all of which will contribute immensely to the advancement of toxicologic and biomolecular research. JF - Toxicologic pathology AU - Dixon, D AU - Johnson, G A AU - Cofer, G P AU - Hedlund, L W AU - Maronpot, R R AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 386 EP - 391 VL - 16 IS - 3 SN - 0192-6233, 0192-6233 KW - Index Medicus KW - Rats KW - Animals KW - Magnetic Resonance Imaging KW - Pathology KW - Toxicology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78542251?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+pathology&rft.atitle=Magnetic+resonance+imaging+%28MRI%29%3A+a+new+tool+in+experimental+toxicologic+pathology.&rft.au=Dixon%2C+D%3BJohnson%2C+G+A%3BCofer%2C+G+P%3BHedlund%2C+L+W%3BMaronpot%2C+R+R&rft.aulast=Dixon&rft.aufirst=D&rft.date=1988-01-01&rft.volume=16&rft.issue=3&rft.spage=386&rft.isbn=&rft.btitle=&rft.title=Toxicologic+pathology&rft.issn=01926233&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-28 N1 - Date created - 1988-12-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Leukemias and occupation in Sweden: a registry-based analysis. AN - 78535865; 3189348 AB - A linked-registry was used to examine systematically, on a national basis, the leukemia incidence in Swedish men by industry and occupation. New associations were observed for chronic lymphocytic leukemia among cloth and pattern cutters and for chronic myelocytic leukemia among brewery workers and motor mechanics. A number of additional findings were consistent with previous observations in other countries. Although etiologic inferences are limited when using linked-registry data, this hypothesis-generating study may provide new clues to the occupational determinants of specific forms of leukemia. JF - American journal of industrial medicine AU - Linet, M S AU - Malker, H S AU - McLaughlin, J K AU - Weiner, J A AU - Stone, B J AU - Blot, W J AU - Ericsson, J L AU - Fraumeni, J F AD - Epidemiology and Biostatistics Program, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 319 EP - 330 VL - 14 IS - 3 SN - 0271-3586, 0271-3586 KW - Index Medicus KW - Registries KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma -- epidemiology KW - Leukemia, Lymphocytic, Chronic, B-Cell -- epidemiology KW - Leukemia, Myeloid, Acute -- epidemiology KW - Humans KW - Leukemia, Myeloid, Acute -- etiology KW - Leukemia, Lymphocytic, Chronic, B-Cell -- etiology KW - Occupations KW - Male KW - Sweden KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma -- etiology KW - Industry KW - Leukemia -- epidemiology KW - Occupational Diseases -- etiology KW - Occupational Diseases -- epidemiology KW - Leukemia -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78535865?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Leukemias+and+occupation+in+Sweden%3A+a+registry-based+analysis.&rft.au=Linet%2C+M+S%3BMalker%2C+H+S%3BMcLaughlin%2C+J+K%3BWeiner%2C+J+A%3BStone%2C+B+J%3BBlot%2C+W+J%3BEricsson%2C+J+L%3BFraumeni%2C+J+F&rft.aulast=Linet&rft.aufirst=M&rft.date=1988-01-01&rft.volume=14&rft.issue=3&rft.spage=319&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-02 N1 - Date created - 1988-12-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Intestinal cancer induced by N-nitroso compounds. AN - 78517367; 3055229 AB - Several N-nitroso compounds induce tumors of the colon, and some induce tumors in other parts of the intestinal tract as well. The nitrosamines that induce colon tumors are beta oxidized n-propyl-nitrosamines. These require metabolic activation, as do 1,2-dimethylhydrazine, azomethane, and azoxymethane, another group of colon carcinogens. Several nitrosoalkylureas induce tumors in rat colons after oral administration, although the monoalkylnitrosoureas are fairly unstable and might not be expected to reach the colon. However, monoalkylnitrosoureas are equally effective with the much more stable dialkylnitrosoureas. Although nitrosomethylurea did not induce colon tumors under these conditions, nitrosoethylurea did, together with nitrosodiethylurea and other nitrosoethylalkylureas. Nitroso-n-butyl-, n-amyl-, n-hexyl-urea, and nitrosohydroxyethylurea also induced colon tumors, but the last, like nitrosoethylurea, also induced tumors of the duodenum and ileum. In most of these experiments male rats were more susceptible to induction of intestinal tumors than female rats. An explanation for the differences between these compounds of similar structure might be found in variations in their ability to alkylate DNA in intestinal cells, or in differences in stability of the alkylated product between the compounds. The physical properties of the compounds might also modulate the process of carcinogenesis, however. JF - Toxicologic pathology AU - Lijinsky, W AD - NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 198 EP - 204 VL - 16 IS - 2 SN - 0192-6233, 0192-6233 KW - Carcinogens KW - 0 KW - Nitroso Compounds KW - Nitrosourea Compounds KW - Index Medicus KW - Rats KW - Animals KW - Intestinal Neoplasms -- pathology KW - Intestinal Neoplasms -- chemically induced KW - Nitrosourea Compounds -- toxicity KW - Organ Specificity KW - Male KW - Female KW - Cricetinae KW - Alkylation KW - Nitroso Compounds -- toxicity KW - Colonic Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78517367?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+pathology&rft.atitle=Intestinal+cancer+induced+by+N-nitroso+compounds.&rft.au=Lijinsky%2C+W&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=16&rft.issue=2&rft.spage=198&rft.isbn=&rft.btitle=&rft.title=Toxicologic+pathology&rft.issn=01926233&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-15 N1 - Date created - 1988-12-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Human immunodeficiency virus as a possible cofactor in the development of fulminant hepatitis B in intravenous drug abusers. AN - 78510976; 3183532 AB - Fulminant hepatitis B virus (HBV) infection is often attributable to co-infection with the delta agent. However, delta produces a variable pattern of illness in HBsAg-positive addicts. It is often not detectable in fulminant cases of hepatitis B, and other cofactors have been conjectured regarding the pathogenesis of fulminant HBV disease. We present the case of an intravenous drug abuser with severe hepatitis B and concomitant infection with human immunodeficiency virus. We conclude that dual infection with these two viruses may be a factor in the development of fulminant hepatitis B in i.v. drug abusers, and might account for the atypical clinical course often observed. JF - Journal of medicine AU - Lange, W R AU - Moore, J D AU - Cibull, M L AU - Brutsche, R L AD - Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 203 EP - 214 VL - 19 IS - 3-4 SN - 0025-7850, 0025-7850 KW - Index Medicus KW - AIDS/HIV KW - Injections, Intravenous KW - Humans KW - Adult KW - Female KW - Hepatitis B -- microbiology KW - HIV -- physiology KW - HIV Seropositivity KW - Substance-Related Disorders KW - Hepatitis B -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78510976?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medicine&rft.atitle=Human+immunodeficiency+virus+as+a+possible+cofactor+in+the+development+of+fulminant+hepatitis+B+in+intravenous+drug+abusers.&rft.au=Lange%2C+W+R%3BMoore%2C+J+D%3BCibull%2C+M+L%3BBrutsche%2C+R+L&rft.aulast=Lange&rft.aufirst=W&rft.date=1988-01-01&rft.volume=19&rft.issue=3-4&rft.spage=203&rft.isbn=&rft.btitle=&rft.title=Journal+of+medicine&rft.issn=00257850&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-12 N1 - Date created - 1988-12-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Magnesium counteracts nickel-induced suppression of T lymphocyte response to concanavalin A. AN - 78506423; 3263555 AB - Effects of nickel and magnesium on the responsiveness of BALB/c mouse spleen lymphocytes to a mitogen, concanavalin A (Con A), were studied using the in vitro 3H-thymidine (TdR) incorporation test. Nickel chloride (NiCl2) and nickel subsulfide (Ni3S2) were found to suppress this response. The control level of TdR incorporation from a magnesium-free medium containing 0.625 microgram Con A/ml into trichloroacetic-acid-precipitable nucleoprotein was depressed by both nickel compounds in a dose-dependent manner to 3% of its original value by 0.25 mumol NiCl2/ml or 0.33 mumol Ni3S2/ml (5 micron particles). Magnesium stimulated TdR incorporation up to a maximum of 200% of the control level at concentrations greater than 2.0 mumol MgCl2/ml. Also, gradual increase of magnesium concentration in the culture medium up to 2.0 mumol/ml attenuated the effects of nickel, restoring the lymphocyte response to Con A to 43% of the control level at 0.25 mumol NiCl2/ml or to 30% at 0.33 mumol Ni3S2/ml. Higher concentrations of magnesium did not further enhance this responsiveness. These data suggest that the effect of magnesium upon early cellular response to nickel observed in vivo [Kasprzak et al.: Carcinogenesis 6: 1161-1166, 1985], which eventually results in a decreased tumor incidence, may be due in part to antagonism by magnesium of nickel suppression of the activity of T lymphocytes. JF - Magnesium AU - Kasprzak, K S AU - Kiser, R F AU - Weislow, O S AD - Inorganic Carcinogenesis Section, National Cancer Institute, Frederick, Md. Y1 - 1988 PY - 1988 DA - 1988 SP - 166 EP - 172 VL - 7 IS - 3 SN - 0252-1156, 0252-1156 KW - magnesium carbonate KW - 0E53J927NA KW - Concanavalin A KW - 11028-71-0 KW - nickel subsulfide KW - 12035-72-2 KW - nickel chloride KW - 696BNE976J KW - Nickel KW - 7OV03QG267 KW - DNA KW - 9007-49-2 KW - Magnesium KW - I38ZP9992A KW - Index Medicus KW - Animals KW - Drug Interactions KW - Spleen -- cytology KW - Mice KW - Mice, Inbred BALB C KW - DNA -- biosynthesis KW - Female KW - T-Lymphocytes -- metabolism KW - Nickel -- pharmacology KW - Magnesium -- pharmacology KW - T-Lymphocytes -- drug effects KW - Concanavalin A -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78506423?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnesium&rft.atitle=Magnesium+counteracts+nickel-induced+suppression+of+T+lymphocyte+response+to+concanavalin+A.&rft.au=Kasprzak%2C+K+S%3BKiser%2C+R+F%3BWeislow%2C+O+S&rft.aulast=Kasprzak&rft.aufirst=K&rft.date=1988-01-01&rft.volume=7&rft.issue=3&rft.spage=166&rft.isbn=&rft.btitle=&rft.title=Magnesium&rft.issn=02521156&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-22 N1 - Date created - 1988-12-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Detection of antibodies to Herpesvirus saimiri late antigens in human sera. AN - 78502400; 2846466 AB - One hundred fifty sera from handlers of squirrel monkeys and 100 sera from individuals who had never handled monkeys were tested by immunofluorescence for antibodies reactive to structural proteins of Herpesvirus saimiri (HVS). Eleven (7.3%) of the occupationally exposed group and 4 (4%) of the noncontact group were seropositive for HVS by immunofluorescence assay, and 10 of these 15 (6.7 and 2%, respectively) were also seropositive for either the major glycoprotein (140 kD) or the major capsid protein (160 kD) of HVS by radioimmunoprecipitation assay. Two sera from handlers of squirrel monkeys, however, recognized many different HVS structural antigens by immunoprecipitation, and it seems unlikely that they could also be cross-reactive antibodies. Since these two sera did not contain antibodies to HVS early antigens or to the nonstructural antigens present in infected owl monkey kidney cells, and follow-up sera collected from the same individuals several months later were negative for antibodies to HVS, these individuals do not appear to have been infected by the virus. The risk that HVS poses to humans appears to be very low. JF - Intervirology AU - Ablashi, D V AU - Dahlberg, J E AU - Cannon, G B AU - Fischetti, G AU - Loeb, W AU - Hinds, W AU - Schatte, C AU - Levine, P H AD - Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Md 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 217 EP - 226 VL - 29 IS - 4 SN - 0300-5526, 0300-5526 KW - Antibodies, Viral KW - 0 KW - Antigens, Viral KW - Viral Proteins KW - Viral Structural Proteins KW - Index Medicus KW - Viral Proteins -- immunology KW - Animals KW - Humans KW - Herpesviridae Infections -- etiology KW - Occupational Diseases -- etiology KW - Animal Husbandry KW - Precipitin Tests KW - Lymphocytes -- microbiology KW - Fluorescent Antibody Technique KW - Saimiri -- microbiology KW - Antibodies, Viral -- isolation & purification KW - Herpesvirus 2, Saimiriine -- isolation & purification KW - Herpesvirus 2, Saimiriine -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78502400?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Intervirology&rft.atitle=Detection+of+antibodies+to+Herpesvirus+saimiri+late+antigens+in+human+sera.&rft.au=Ablashi%2C+D+V%3BDahlberg%2C+J+E%3BCannon%2C+G+B%3BFischetti%2C+G%3BLoeb%2C+W%3BHinds%2C+W%3BSchatte%2C+C%3BLevine%2C+P+H&rft.aulast=Ablashi&rft.aufirst=D&rft.date=1988-01-01&rft.volume=29&rft.issue=4&rft.spage=217&rft.isbn=&rft.btitle=&rft.title=Intervirology&rft.issn=03005526&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-20 N1 - Date created - 1988-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Studies of cancer among the Japanese A-bomb survivors. AN - 78501193; 3179774 AB - Studies of cancer among the Japanese survivors of the A-bombs dropped on Nagasaki and Hiroshima are the major source of information on radiation carcinogenesis in humans. They have already made important contributions to the estimation of the risk of radiation-induced cancer and to our understanding of key factors influencing risk, especially tissue sensitivity, age at exposure, and the temporal distribution of radiogenic cancers. The size of the exposed population still surviving virtually guarantees the continued productivity of the research conducted in Hiroshima and Nagasaki. It should not be supposed, however, that these studies will ever provide all the information needed for radiation protection standards or risk estimation. The experience of the A-bomb survivors simply does not include all important aspects of radiation exposure for which information is needed. Moreover, despite the size of the sample remaining under study, it is most unlikely that direct, empirical estimates can be made that will remove the necessity for dependence upon mathematical models to derive estimates of risk in the low-dose region. JF - Cancer investigation AU - Beebe, G W AD - Clinical Epidemiology Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 417 EP - 426 VL - 6 IS - 4 SN - 0735-7907, 0735-7907 KW - Index Medicus KW - Infant KW - Risk KW - Radiation Dosage KW - Age Factors KW - Humans KW - Adult KW - Infant, Newborn KW - Middle Aged KW - Child KW - Adolescent KW - Time Factors KW - Japan KW - Child, Preschool KW - Nuclear Warfare KW - Neoplasms, Radiation-Induced -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78501193?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+investigation&rft.atitle=Studies+of+cancer+among+the+Japanese+A-bomb+survivors.&rft.au=Beebe%2C+G+W&rft.aulast=Beebe&rft.aufirst=G&rft.date=1988-01-01&rft.volume=6&rft.issue=4&rft.spage=417&rft.isbn=&rft.btitle=&rft.title=Cancer+investigation&rft.issn=07357907&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-25 N1 - Date created - 1988-11-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Toxicity and carcinogenicity of rotenone given in the feed to F344/N rats and B6C3F1 mice for up to two years. AN - 78496696; 3181037 AB - Toxicity and carcinogenicity studies of rotenone were conducted in F344/N rats and B6C3F1 mice. Groups of 50 rats and 50 mice of each sex were given rotenone in their diet for up to 103 weeks. The doses were 0, 38, and 75 ppm for rats and 0, 600, and 1,200 ppm for mice. Reduction in body weight gain occurred in male and female mice given rotenone. No effects on survival were observed for rats of either sex or female mice. Survival of male mice at 1,200 ppm was significantly greater than that of controls (47/50 vs. 29/50). There were no observed nonneoplastic effects due to rotenone, and for male and female mice no neoplasms were induced by rotenone. Parathyroid adenomas occurred at a higher incidence (4/44) in male rats at 75 ppm than in the controls (1/41). Because these tumors are rare (historical rate in NTP studies is 0.3%), the increase in the incidence of these benign tumors may have been related to rotenone administration. Hepatocellular neoplasms were reduced (p less than 0.01) in males receiving 1,200 ppm 1/50 relative to controls 12/47. Because this low rate of liver tumors is unusual in male B6C3F1 mice, this decrease was considered to be related to rotenone administration. JF - Drug and chemical toxicology AU - Abdo, K M AU - Eustis, S L AU - Haseman, J AU - Huff, J E AU - Peters, A AU - Persing, R AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 225 EP - 235 VL - 11 IS - 3 SN - 0148-0545, 0148-0545 KW - Carcinogens KW - 0 KW - Rotenone KW - 03L9OT429T KW - Index Medicus KW - Eating -- drug effects KW - Animals KW - Soft Tissue Neoplasms -- pathology KW - Sex Factors KW - Mice KW - Parathyroid Neoplasms -- pathology KW - Rats KW - Mice, Inbred Strains KW - Rats, Inbred F344 KW - Soft Tissue Neoplasms -- chemically induced KW - Body Weight -- drug effects KW - Parathyroid Neoplasms -- chemically induced KW - Diet KW - Time Factors KW - Female KW - Male KW - Rotenone -- toxicity KW - Rotenone -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78496696?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+and+chemical+toxicology&rft.atitle=Toxicity+and+carcinogenicity+of+rotenone+given+in+the+feed+to+F344%2FN+rats+and+B6C3F1+mice+for+up+to+two+years.&rft.au=Abdo%2C+K+M%3BEustis%2C+S+L%3BHaseman%2C+J%3BHuff%2C+J+E%3BPeters%2C+A%3BPersing%2C+R&rft.aulast=Abdo&rft.aufirst=K&rft.date=1988-01-01&rft.volume=11&rft.issue=3&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Drug+and+chemical+toxicology&rft.issn=01480545&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-02 N1 - Date created - 1988-12-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Mechanisms of metal-induced renal cell injury: roles of high-affinity metal-binding proteins. AN - 78487940; 3053040 JF - Contributions to nephrology AU - Fowler, B A AD - National Institute of Environmental Health Sciences, Research Triangle Park, N.C. Y1 - 1988 PY - 1988 DA - 1988 SP - 83 EP - 92 VL - 64 SN - 0302-5144, 0302-5144 KW - Carrier Proteins KW - 0 KW - Metals KW - lead-binding proteins KW - Metallothionein KW - 9038-94-2 KW - Index Medicus KW - Animals KW - Carrier Proteins -- metabolism KW - Humans KW - Microscopy, Electron KW - Metallothionein -- metabolism KW - Kidney -- metabolism KW - Metals -- adverse effects KW - Kidney -- drug effects KW - Metals -- metabolism KW - Kidney -- ultrastructure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78487940?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Contributions+to+nephrology&rft.atitle=Mechanisms+of+metal-induced+renal+cell+injury%3A+roles+of+high-affinity+metal-binding+proteins.&rft.au=Fowler%2C+B+A&rft.aulast=Fowler&rft.aufirst=B&rft.date=1988-01-01&rft.volume=64&rft.issue=&rft.spage=83&rft.isbn=&rft.btitle=&rft.title=Contributions+to+nephrology&rft.issn=03025144&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-12-12 N1 - Date created - 1988-12-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Adolescent alcohol and drug abuse and its consequences--an overview. AN - 78476041; 3052038 JF - The American journal of drug and alcohol abuse AU - Czechowicz, D AD - Office of Science, National Institute on Drug Abuse, Rockville, Maryland 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 189 EP - 197 VL - 14 IS - 2 SN - 0095-2990, 0095-2990 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - United States KW - Risk Factors KW - Humans KW - Accidents -- mortality KW - Marijuana Abuse -- epidemiology KW - Child KW - Adolescent KW - Adolescent Behavior KW - Alcoholism -- epidemiology KW - Substance-Related Disorders -- complications KW - Alcoholism -- complications KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78476041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+drug+and+alcohol+abuse&rft.atitle=Adolescent+alcohol+and+drug+abuse+and+its+consequences--an+overview.&rft.au=Czechowicz%2C+D&rft.aulast=Czechowicz&rft.aufirst=D&rft.date=1988-01-01&rft.volume=14&rft.issue=2&rft.spage=189&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+drug+and+alcohol+abuse&rft.issn=00952990&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-03 N1 - Date created - 1988-11-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Smoking as an issue in alcohol and drug abuse treatment. AN - 78474299; 3051913 AB - Little attention has been given to the role of tobacco dependence within alcohol and drug abuse treatment. Yet, smoking behavior appears to be interrelated with the use of alcohol and other drugs. This interrelationship is explored, and the role of smoking cessation within alcohol and drug abuse treatment is considered. Areas for future research on this topic are identified. Addictive disorders are generally thought to include alcohol abuse, drug abuse, smoking, overeating, and, sometimes, gambling and caffeine dependence. While some attention has been paid to the common etiological roots of various addictive disorders, relatively little systematic attention has been paid to commonalities in their treatment and especially to the treatment of multiple disorders in the same individuals. The one significant exception is alcohol abuse and drug abuse. Of the other addictive disorders, tobacco dependence has been most closely interrelated with alcohol and drug abuse. Yet, little attention has been given to tobacco dependence within alcohol and drug abuse treatment. This paper will focus on smoking in relationship with alcohol and drug abuse, and will consider the possible role of smoking cessation treatment within the context of alcohol and drug abuse treatment. First, background regarding the interrelationship of alcohol and drug abuse is explored. Then, the relationship of smoking with other substance use is considered, followed by a review of special concerns related to smoking among alcohol and drug abuse clients. Next, the current status of smoking cessation within alcohol and drug abuse treatment is addressed. Finally, implications are considered. JF - Addictive behaviors AU - Battjes, R J AD - National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 225 EP - 230 VL - 13 IS - 3 SN - 0306-4603, 0306-4603 KW - Index Medicus KW - Combined Modality Therapy KW - Humans KW - Alcoholism -- rehabilitation KW - Smoking -- therapy KW - Smoking -- psychology KW - Substance-Related Disorders -- rehabilitation KW - Substance-Related Disorders -- psychology KW - Alcoholism -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78474299?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addictive+behaviors&rft.atitle=Smoking+as+an+issue+in+alcohol+and+drug+abuse+treatment.&rft.au=Battjes%2C+R+J&rft.aulast=Battjes&rft.aufirst=R&rft.date=1988-01-01&rft.volume=13&rft.issue=3&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Addictive+behaviors&rft.issn=03064603&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-16 N1 - Date created - 1988-11-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Benzodiazepine, barbiturate, ethanol and hypnotic steroid hormone modulation of GABA-mediated chloride ion transport in rat brain synaptoneurosomes. AN - 78472075; 2902744 JF - Advances in biochemical psychopharmacology AU - Morrow, A L AU - Suzdak, P D AU - Paul, S M AD - Section on Molecular Pharmacology, National Institute of Mental Health, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 247 EP - 261 VL - 45 SN - 0065-2229, 0065-2229 KW - Anti-Anxiety Agents KW - 0 KW - Barbiturates KW - Chlorides KW - Hypnotics and Sedatives KW - Receptors, GABA-A KW - Steroids KW - Benzodiazepines KW - 12794-10-4 KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Synaptosomes -- drug effects KW - Brain -- drug effects KW - In Vitro Techniques KW - Steroids -- pharmacology KW - Brain -- metabolism KW - Drug Synergism KW - Synaptosomes -- metabolism KW - Male KW - Anti-Anxiety Agents -- pharmacology KW - Receptors, GABA-A -- physiology KW - Ethanol -- pharmacology KW - Barbiturates -- pharmacology KW - Receptors, GABA-A -- drug effects KW - Chlorides -- metabolism KW - Hypnotics and Sedatives -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78472075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+biochemical+psychopharmacology&rft.atitle=Benzodiazepine%2C+barbiturate%2C+ethanol+and+hypnotic+steroid+hormone+modulation+of+GABA-mediated+chloride+ion+transport+in+rat+brain+synaptoneurosomes.&rft.au=Morrow%2C+A+L%3BSuzdak%2C+P+D%3BPaul%2C+S+M&rft.aulast=Morrow&rft.aufirst=A&rft.date=1988-01-01&rft.volume=45&rft.issue=&rft.spage=247&rft.isbn=&rft.btitle=&rft.title=Advances+in+biochemical+psychopharmacology&rft.issn=00652229&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-23 N1 - Date created - 1988-11-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Rapid induction of lorazepam dependence and reversal with flumazenil. AN - 78461869; 3139949 AB - Benzodiazepine tolerance, dependence and withdrawal are well established clinical entities although the pharmacological basis for these are still unclear. Recent data suggest that the primary event may be a change in efficacy at the benzodiazepine receptor. The present study demonstrates the rapid development of tolerance and dependence to lorazepam, defines its pharmacology in more detail, and shows that it may be rapidly reversed by treatment with the benzodiazepine antagonist flumazenil. These observations argue in favour of a receptor efficacy change underlying benzodiazepine tolerance and withdrawal and suggest a potential pharmacological treatment for this common and disabling clinical problem. JF - Life sciences AU - Nutt, D J AU - Costello, M J AD - Laboratory of Clinical Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 1045 EP - 1053 VL - 43 IS - 13 SN - 0024-3205, 0024-3205 KW - Carbolines KW - 0 KW - Flumazenil KW - 40P7XK9392 KW - FG 7142 KW - 60PO70N1BP KW - beta-carboline-3-carboxylic acid methyl ester KW - I2A008F6YL KW - Lorazepam KW - O26FZP769L KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Seizures -- physiopathology KW - Mice KW - Carbolines -- pharmacology KW - Male KW - Substance-Related Disorders -- physiopathology KW - Flumazenil -- therapeutic use KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78461869?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Rapid+induction+of+lorazepam+dependence+and+reversal+with+flumazenil.&rft.au=Nutt%2C+D+J%3BCostello%2C+M+J&rft.aulast=Nutt&rft.aufirst=D&rft.date=1988-01-01&rft.volume=43&rft.issue=13&rft.spage=1045&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-09 N1 - Date created - 1988-11-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - An introduction to compulsory treatment for drug abuse: clinical practice and research. AN - 78458651; 3140026 JF - NIDA research monograph AU - Leukefeld, C G AU - Tims, F M AD - National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 7 VL - 86 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - AIDS/HIV KW - United States KW - Injections, Intravenous KW - Humans KW - Acquired Immunodeficiency Syndrome -- transmission KW - Substance-Related Disorders -- therapy KW - Commitment of Mentally Ill UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78458651?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=An+introduction+to+compulsory+treatment+for+drug+abuse%3A+clinical+practice+and+research.&rft.au=Leukefeld%2C+C+G%3BTims%2C+F+M&rft.aulast=Leukefeld&rft.aufirst=C&rft.date=1988-01-01&rft.volume=86&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-08 N1 - Date created - 1988-11-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Range of toxicity of topical cysteamine in rabbit eyes. AN - 78458136; 3171336 AB - Cysteamine eye drops in concentrations from 0.1% to 10% were tested in albino rabbits in both short and long-term experiments. Toxicity, consisting of an inflammatory response, was proportional to the cysteamine concentration and absent at concentrations at or below 0.5%. JF - Journal of ocular pharmacology AU - Jain, S AU - Kuwabara, T AU - Gahl, W A AU - Kaiser-Kupfer, M I AD - Clinical Branch, National Eye Institute, Bethesda, Maryland. Y1 - 1988 PY - 1988 DA - 1988 SP - 127 EP - 131 VL - 4 IS - 2 SN - 8756-3320, 8756-3320 KW - Ophthalmic Solutions KW - 0 KW - Cysteamine KW - 5UX2SD1KE2 KW - Index Medicus KW - Animals KW - Rabbits KW - Male KW - Female KW - Cysteamine -- toxicity KW - Cysteamine -- administration & dosage KW - Eye -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78458136?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+ocular+pharmacology&rft.atitle=Range+of+toxicity+of+topical+cysteamine+in+rabbit+eyes.&rft.au=Jain%2C+S%3BKuwabara%2C+T%3BGahl%2C+W+A%3BKaiser-Kupfer%2C+M+I&rft.aulast=Jain&rft.aufirst=S&rft.date=1988-01-01&rft.volume=4&rft.issue=2&rft.spage=127&rft.isbn=&rft.btitle=&rft.title=Journal+of+ocular+pharmacology&rft.issn=87563320&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-04 N1 - Date created - 1988-11-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Hormone therapy for prostate cancer: results of the Veterans Administration Cooperative Urological Research Group studies. AN - 78458109; 3050535 AB - Between 1960 and 1975, the Veterans Administration Cooperative Urological Research Group conducted a consecutive series of 3 major randomized clinical trials comparing various endocrine treatments for newly diagnosed prostate cancer patients. Six major conclusions concerning hormonal treatment emerged from these studies: 1) increased hazard of cardiovascular death after therapy with 5 mg diethylstilbestrol (DES); 2) orchiectomy plus DES no better than orchiectomy or DES alone; 3) equivalent effect of 1.0 and 5.0 mg DES on cancer; 4) reduced cardiovascular hazard from therapy with 1.0 mg DES; 5) Premarin and Provera no better than 1.0 mg DES at doses studied; 6) decisions about hormone treatment at diagnosis dependent on patient characteristics, mainly age and Gleason grade. In this paper, these studies are reviewed briefly and data are presented to support these conclusions. Some tentative treatment recommendations are proposed. JF - NCI monographs : a publication of the National Cancer Institute AU - Byar, D P AU - Corle, D K AD - Division of Cancer Prevention and Control, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 165 EP - 170 IS - 7 SN - 0893-2751, 0893-2751 KW - Diethylstilbestrol KW - 731DCA35BT KW - Index Medicus KW - Humans KW - Clinical Trials as Topic KW - Cardiovascular Diseases -- chemically induced KW - Male KW - Orchiectomy KW - Prostatic Neoplasms -- mortality KW - Diethylstilbestrol -- therapeutic use KW - Diethylstilbestrol -- adverse effects KW - Prostatic Neoplasms -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78458109?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.atitle=Hormone+therapy+for+prostate+cancer%3A+results+of+the+Veterans+Administration+Cooperative+Urological+Research+Group+studies.&rft.au=Byar%2C+D+P%3BCorle%2C+D+K&rft.aulast=Byar&rft.aufirst=D&rft.date=1988-01-01&rft.volume=&rft.issue=7&rft.spage=165&rft.isbn=&rft.btitle=&rft.title=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.issn=08932751&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-03 N1 - Date created - 1988-11-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Basic issues pertaining to the effectiveness of methadone maintenance treatment. AN - 78455786; 3140030 JF - NIDA research monograph AU - Ball, J C AU - Corty, E AD - National Institute on Drug Abuse, Addiction Research Center, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 178 EP - 191 VL - 86 SN - 1046-9516, 1046-9516 KW - Methadone KW - UC6VBE7V1Z KW - Index Medicus KW - United States KW - Humans KW - Criminal Law KW - Counseling KW - Male KW - Methadone -- therapeutic use KW - Heroin Dependence -- rehabilitation KW - Heroin Dependence -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78455786?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Basic+issues+pertaining+to+the+effectiveness+of+methadone+maintenance+treatment.&rft.au=Ball%2C+J+C%3BCorty%2C+E&rft.aulast=Ball&rft.aufirst=J&rft.date=1988-01-01&rft.volume=86&rft.issue=&rft.spage=178&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-08 N1 - Date created - 1988-11-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Epidemiologic studies--Kaposi's sarcoma vs. opportunistic infections among homosexual men with AIDS. AN - 78455738; 3140024 JF - NIDA research monograph AU - Haverkos, H W AD - Clinical Medicine Branch, National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 96 EP - 105 VL - 83 SN - 1046-9516, 1046-9516 KW - Nitrites KW - 0 KW - Index Medicus KW - AIDS/HIV KW - United States KW - Epidemiologic Methods KW - Nitrites -- adverse effects KW - Humans KW - Surveys and Questionnaires KW - Male KW - Substance-Related Disorders -- epidemiology KW - Opportunistic Infections -- epidemiology KW - Acquired Immunodeficiency Syndrome -- complications KW - Opportunistic Infections -- etiology KW - Acquired Immunodeficiency Syndrome -- epidemiology KW - Sarcoma, Kaposi -- epidemiology KW - Homosexuality KW - Sarcoma, Kaposi -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78455738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Epidemiologic+studies--Kaposi%27s+sarcoma+vs.+opportunistic+infections+among+homosexual+men+with+AIDS.&rft.au=Haverkos%2C+H+W&rft.aulast=Haverkos&rft.aufirst=H&rft.date=1988-01-01&rft.volume=83&rft.issue=&rft.spage=96&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-09 N1 - Date created - 1988-11-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - 3-Ethoxy-beta-carboline: a high affinity benzodiazepine receptor ligand with partial inverse agonist properties. AN - 78455736; 2845211 AB - 3-Ethoxy-beta-carboline binds with high affinity to benzodiazepine receptors in the central nervous system (Ki approximately equal to 10.1, 15.3, and 25.3 nM in rat cerebellum, cerebral cortex, and hippocampus, respectively). This compound has pharmacological actions reminiscent of benzodiazepine receptor partial inverse agonists such as FG 7142 and 3-carboethoxy-beta-carboline. Thus, while not a convulsant, 3-ethoxy-beta-carboline potentiated the convulsant actions of pentylenetetrazole in mice. Furthermore, this compound reduced both the time spent and the total entries in the open arms of an elevated plus maze and also inhibited stress-induced ulcer formation, effects that are also observed with benzodiazepine receptor inverse agonists. These findings suggest that 3-ethoxy-beta-carboline is a partial inverse agonist at benzodiazepine receptors which may prove useful for in vivo studies since it has a higher affinity for benzodiazepine receptors and better solubility than the commonly used partial inverse agonist FG 7142. Furthermore, 3-ethoxy-beta-carboline appears to be less vulnerable to metabolic degradation than ester analogs with a similar pharmacological profile such as 3-carboethoxy-beta-carboline. JF - Life sciences AU - Trullas, R AU - Ginter, H AU - Jackson, B AU - Skolnick, P AU - Allen, M S AU - Hagen, T J AU - Cook, J M AD - Laboratory of Neuroscience, NIDDK, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 1189 EP - 1197 VL - 43 IS - 15 SN - 0024-3205, 0024-3205 KW - Carbolines KW - 0 KW - Convulsants KW - Ligands KW - Receptors, GABA-A KW - Flumazenil KW - 40P7XK9392 KW - FG 7142 KW - 60PO70N1BP KW - 3-ethoxy-beta-carboline KW - 91985-81-8 KW - Index Medicus KW - Seizures -- chemically induced KW - Animals KW - Solubility KW - Flumazenil -- metabolism KW - Cerebral Cortex -- metabolism KW - Stress, Psychological -- physiopathology KW - Convulsants -- pharmacology KW - Hippocampus -- metabolism KW - Peptic Ulcer -- physiopathology KW - Cerebellum -- metabolism KW - Rats KW - Binding, Competitive KW - In Vitro Techniques KW - Drug Synergism KW - Carbolines -- physiology KW - Receptors, GABA-A -- metabolism KW - Brain -- metabolism KW - Carbolines -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78455736?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=3-Ethoxy-beta-carboline%3A+a+high+affinity+benzodiazepine+receptor+ligand+with+partial+inverse+agonist+properties.&rft.au=Trullas%2C+R%3BGinter%2C+H%3BJackson%2C+B%3BSkolnick%2C+P%3BAllen%2C+M+S%3BHagen%2C+T+J%3BCook%2C+J+M&rft.aulast=Trullas&rft.aufirst=R&rft.date=1988-01-01&rft.volume=43&rft.issue=15&rft.spage=1189&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-17 N1 - Date created - 1988-11-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Nitrite inhalants: contemporary patterns of abuse. AN - 78452812; 3140023 JF - NIDA research monograph AU - Lange, W R AU - Dax, E M AU - Haertzen, C A AU - Snyder, F R AU - Jaffe, J H AD - Addiction Research Center, National Institute on Drug Abuse, Francis Scott Key Medical Center, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 86 EP - 95 VL - 83 SN - 1046-9516, 1046-9516 KW - Nitrites KW - 0 KW - Index Medicus KW - United States KW - Baltimore KW - Humans KW - Adult KW - Homosexuality KW - Adolescent KW - Administration, Inhalation KW - Male KW - Female KW - Nitrites -- administration & dosage KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78452812?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Nitrite+inhalants%3A+contemporary+patterns+of+abuse.&rft.au=Lange%2C+W+R%3BDax%2C+E+M%3BHaertzen%2C+C+A%3BSnyder%2C+F+R%3BJaffe%2C+J+H&rft.aulast=Lange&rft.aufirst=W&rft.date=1988-01-01&rft.volume=83&rft.issue=&rft.spage=86&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-09 N1 - Date created - 1988-11-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Compulsory treatment: a review of findings. AN - 78451439; 3140033 JF - NIDA research monograph AU - Leukefeld, C G AU - Tims, F M AD - National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 236 EP - 251 VL - 86 SN - 1046-9516, 1046-9516 KW - Methadone KW - UC6VBE7V1Z KW - Index Medicus KW - United States KW - Methadone -- therapeutic use KW - Length of Stay KW - Humans KW - Cost-Benefit Analysis KW - Adult KW - Male KW - Substance-Related Disorders -- therapy KW - Jurisprudence KW - Criminal Law KW - Substance-Related Disorders -- economics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78451439?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Compulsory+treatment%3A+a+review+of+findings.&rft.au=Leukefeld%2C+C+G%3BTims%2C+F+M&rft.aulast=Leukefeld&rft.aufirst=C&rft.date=1988-01-01&rft.volume=86&rft.issue=&rft.spage=236&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-11-08 N1 - Date created - 1988-11-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: quality-control guidelines and response categories. AN - 78425752; 3416839 AB - A data-based approach to formulating quality-control criteria for the mouse lymphoma cell forward mutation assay is described. Quality-control guidelines for solvent controls, positive controls, and compound-treated cultures were developed based on analysis of over 800 experiments. Frequency distributions of experimental parameters of control cultures, such as mutant frequencies, cloning efficiencies, and suspension growths, were examined. Cloning efficiency and relative total growth affected the variability only when the test chemical was highly toxic. This information was used to generate the quality-control criteria, which were applied to an experiment before it was evaluated for a response. The response categories for classifying the effect of test chemicals on the assay system are defined in terms of (1) the statistically significant differences in average mutant frequency between solvent control cultures and cultures exposed to a chemical and (2) the trend of the dose-related responses. JF - Environmental and molecular mutagenesis AU - Caspary, W J AU - Lee, Y J AU - Poulton, S AU - Myhr, B C AU - Mitchell, A D AU - Rudd, C J AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. Y1 - 1988 PY - 1988 DA - 1988 SP - 19 EP - 36 VL - 12 Suppl 13 SN - 0893-6692, 0893-6692 KW - Mutagens KW - 0 KW - Solvents KW - Index Medicus KW - Evaluation Studies as Topic KW - Animals KW - Tumor Cells, Cultured KW - Solvents -- standards KW - Mice KW - Leukemia L5178 KW - Quality Control KW - Mutation KW - Mutagenicity Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78425752?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+and+molecular+mutagenesis&rft.atitle=Evaluation+of+the+L5178Y+mouse+lymphoma+cell+mutagenesis+assay%3A+quality-control+guidelines+and+response+categories.&rft.au=Caspary%2C+W+J%3BLee%2C+Y+J%3BPoulton%2C+S%3BMyhr%2C+B+C%3BMitchell%2C+A+D%3BRudd%2C+C+J&rft.aulast=Caspary&rft.aufirst=W&rft.date=1988-01-01&rft.volume=12+Suppl+13&rft.issue=&rft.spage=19&rft.isbn=&rft.btitle=&rft.title=Environmental+and+molecular+mutagenesis&rft.issn=08936692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-21 N1 - Date created - 1988-10-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: interlaboratory reproducibility and assessment. AN - 78421086; 3416840 AB - The L5178Y mouse lymphoma cell mutagenesis assay is used to detect the mutagenic activity of chemicals in a mammalian cell system. To evaluate this assay we compared the results of assays performed independently on 63 chemicals by laboratories at SRI International and Litton Bionetics, Inc. The two laboratories used similar protocols. The solvent and positive control mutant frequencies and cloning efficiencies obtained by the two laboratories were similar, which justified the use of the same quality-control criteria and analytical procedures for analyzing the results from both laboratories. The rate of concordance between the two laboratories was 92% for tests in the absence of S9 activation and 95% for tests in its presence. The results of the assays agreed for 57 of the 63 chemicals; three chemicals could not be compared because there were questionable calls in at least one of the laboratories; the results disagreed for the three remaining chemicals. The concordance rate for these overall assay evaluations was 95%. The interlaboratory concordance rates were similar to concordance rates for replicate experiments within the laboratories (96% at LBI, 94% at SRI). The mouse lymphoma cell mutagenicity results are concordant with the rodent chronic assay results in 78% of 50 chemicals and with the Salmonella assay results in 79% of 56 chemicals. Fifteen carcinogens were examined for genotoxic effects in mouse lymphoma, Salmonella, Chinese hamster ovary (CHO) chromosomal aberration, and CHO sister chromatid exchange assay. Eight of these were positive in all four assays. Of the seven noncarcinogens that were tested in these four assays, none was negative in all four. The main conclusion to be drawn from this study is that the mouse lymphoma cell forward mutation assay, as performed and evaluated in this study, detects chemical mutagenicity in a manner that is highly consistent with other genetic endpoints as well as rodent carcinogenicity studies. Thus the assay quality control and response criteria established in this study led not only to a high degree of reproducibility but also to an apparently reliable detection of mutagenic activity. JF - Environmental and molecular mutagenesis AU - Caspary, W J AU - Daston, D S AU - Myhr, B C AU - Mitchell, A D AU - Rudd, C J AU - Lee, P S AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. Y1 - 1988 PY - 1988 DA - 1988 SP - 195 EP - 229 VL - 12 Suppl 13 SN - 0893-6692, 0893-6692 KW - Mutagens KW - 0 KW - Index Medicus KW - Evaluation Studies as Topic KW - Clone Cells KW - Animals KW - Tumor Cells, Cultured KW - Cell Count KW - Dose-Response Relationship, Drug KW - Chemical Precipitation KW - Predictive Value of Tests KW - Mice KW - Leukemia L5178 KW - Quality Control KW - Mutation KW - Mutagenicity Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78421086?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+and+molecular+mutagenesis&rft.atitle=Evaluation+of+the+L5178Y+mouse+lymphoma+cell+mutagenesis+assay%3A+interlaboratory+reproducibility+and+assessment.&rft.au=Caspary%2C+W+J%3BDaston%2C+D+S%3BMyhr%2C+B+C%3BMitchell%2C+A+D%3BRudd%2C+C+J%3BLee%2C+P+S&rft.aulast=Caspary&rft.aufirst=W&rft.date=1988-01-01&rft.volume=12+Suppl+13&rft.issue=&rft.spage=195&rft.isbn=&rft.btitle=&rft.title=Environmental+and+molecular+mutagenesis&rft.issn=08936692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-21 N1 - Date created - 1988-10-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Lack of a relationship between immune function and chemically induced hepatocarcinogenesis in B6C3F1 mice. AN - 78406695; 3262012 AB - The relationship between immune function and chemically induced hepatocarcinogenesis was studied employing an in vivo murine model. Neonatal B6C3F1 mice were given a single carcinogenic dose of diethylnitrosamine (DEN) and the time-response kinetics for the early (foci of alteration) and late (adenomas/carcinomas) phases of hepatocellular carcinogenesis were compared to changes in hematopoiesis and immune functions associated with immune surveillance and natural resistance. Increases in hematopoiesis occurred just prior to or concurrent with the appearance of hepatocellular carcinomas, while increased macrophage and natural killer cell cytotoxicity and suppression of cell-mediated immunity occurred following tumor appearance and progressed with increasing tumor burden. Neither immunological nor hematopoietic changes were associated with early phases of hepatocarcinogenesis, as monitored by the appearance of altered hepatocellular foci. Although changes in hematopoiesis may represent an early indicator for hepatocarcinogenesis in the mouse tumor model, the data suggest that altered immune surveillance and natural resistance are not factors in the development of chemically induced hepatocellular tumors, and the changes in immune function are probably secondary to tumor development. JF - Cancer immunology, immunotherapy : CII AU - Germolec, D R AU - Maronpot, R R AU - Ackermann, M F AU - Vore, S J AU - Dittrich, K AU - Rosenthal, G J AU - Luster, M I AD - Systemic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 121 EP - 127 VL - 27 IS - 2 SN - 0340-7004, 0340-7004 KW - Diethylnitrosamine KW - 3IQ78TTX1A KW - Index Medicus KW - Animals KW - Mice, Inbred C57BL KW - Mice, Inbred C3H KW - Mice KW - Hematopoiesis KW - T-Lymphocytes -- immunology KW - Male KW - Killer Cells, Natural -- immunology KW - Female KW - Liver Neoplasms, Experimental -- immunology KW - Liver Neoplasms, Experimental -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78406695?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+immunology%2C+immunotherapy+%3A+CII&rft.atitle=Lack+of+a+relationship+between+immune+function+and+chemically+induced+hepatocarcinogenesis+in+B6C3F1+mice.&rft.au=Germolec%2C+D+R%3BMaronpot%2C+R+R%3BAckermann%2C+M+F%3BVore%2C+S+J%3BDittrich%2C+K%3BRosenthal%2C+G+J%3BLuster%2C+M+I&rft.aulast=Germolec&rft.aufirst=D&rft.date=1988-01-01&rft.volume=27&rft.issue=2&rft.spage=121&rft.isbn=&rft.btitle=&rft.title=Cancer+immunology%2C+immunotherapy+%3A+CII&rft.issn=03407004&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-24 N1 - Date created - 1988-10-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Calcium agonist (BayK 8644) augments voltage-sensitive calcium currents but not synaptic transmission in cultured mouse spinal cord neurons. AN - 78403207; 2458275 AB - The effects of the calcium agonist, BayK 8644, and other agents upon voltage-dependent calcium conductance (VSCC) and evoked synaptic activity were studied in cultured mouse spinal cord and dorsal root ganglion neurons. As expected, BayK 8644 increased the VSCC corresponding to L channels. It had relatively little effect on evoked synaptic activity; the small but statistically significant effect that was noted was a decrease. Nitrendipine had either no effect or an increase with no statistically significant effect being seen with regard to synaptic activity over the population sampled. An increased extracellular Ca++ concentration increased both VSCC and synaptic activity. We conclude that VSCC with L channel properties are probably not involved in transmitter release produced by action potentials in the central synapses occurring in the dissociated mouse spinal cord cell culture system. JF - Experimental brain research AU - Yu, C AU - Jia, M AU - Litzinger, M AU - Nelson, P G AD - Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 467 EP - 474 VL - 71 IS - 3 SN - 0014-4819, 0014-4819 KW - Tetraethylammonium Compounds KW - 0 KW - Tetrodotoxin KW - 4368-28-9 KW - 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester KW - 71145-03-4 KW - Nitrendipine KW - 9B627AW319 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Nitrendipine -- pharmacology KW - Animals KW - Cells, Cultured KW - Tetraethylammonium Compounds -- pharmacology KW - Mice KW - Membrane Potentials -- drug effects KW - Action Potentials -- drug effects KW - Tetrodotoxin -- pharmacology KW - Electric Stimulation KW - Ganglia, Spinal -- cytology KW - Ganglia, Spinal -- physiology KW - Synaptic Transmission -- drug effects KW - Calcium -- physiology KW - 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester -- pharmacology KW - Ganglia, Spinal -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78403207?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+brain+research&rft.atitle=Calcium+agonist+%28BayK+8644%29+augments+voltage-sensitive+calcium+currents+but+not+synaptic+transmission+in+cultured+mouse+spinal+cord+neurons.&rft.au=Yu%2C+C%3BJia%2C+M%3BLitzinger%2C+M%3BNelson%2C+P+G&rft.aulast=Yu&rft.aufirst=C&rft.date=1988-01-01&rft.volume=71&rft.issue=3&rft.spage=467&rft.isbn=&rft.btitle=&rft.title=Experimental+brain+research&rft.issn=00144819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-27 N1 - Date created - 1988-10-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Fenfluramine-induced suppression of food intake and locomotor activity is differentially altered by the selective type A monoamine oxidase inhibitor clorgyline. AN - 78401817; 3137616 AB - Administration of fenfluramine to rats produced decreases in 1-h food intake and locomotor activity. Short-term (2-6 days) or long-term (21-25 days) treatment with the monoamine oxidase (MAO) type A inhibiting antidepressant clorgyline potentiated fenfluramine-induced suppression of food intake but did not affect fenfluramine-induced suppression of locomotor activity. Although daily (4 h) food intake was not significantly less in clorgyline-treated animals relative to saline-treated controls, body weight gain was significantly less in clorgyline-treated animals relative to controls. These findings demonstrate a differential effect of clorgyline treatment on fenfluramine-induced suppression of food intake and locomotor activity. JF - Psychopharmacology AU - Aulakh, C S AU - Hill, J L AU - Wozniak, K M AU - Murphy, D L AD - Clinical Neuropharmacology Branch, National Institute of Mental Health, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 313 EP - 317 VL - 95 IS - 3 SN - 0033-3158, 0033-3158 KW - Propylamines KW - 0 KW - Fenfluramine KW - 2DS058H2CF KW - Clorgyline KW - LYJ16FZU9Q KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Drug Interactions KW - Body Weight -- drug effects KW - Time Factors KW - Male KW - Eating -- drug effects KW - Propylamines -- pharmacology KW - Motor Activity -- drug effects KW - Clorgyline -- pharmacology KW - Fenfluramine -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78401817?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Fenfluramine-induced+suppression+of+food+intake+and+locomotor+activity+is+differentially+altered+by+the+selective+type+A+monoamine+oxidase+inhibitor+clorgyline.&rft.au=Aulakh%2C+C+S%3BHill%2C+J+L%3BWozniak%2C+K+M%3BMurphy%2C+D+L&rft.aulast=Aulakh&rft.aufirst=C&rft.date=1988-01-01&rft.volume=95&rft.issue=3&rft.spage=313&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-11 N1 - Date created - 1988-10-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Estramustine phosphate (estracyt) following androgens in men with refractory stage D2 prostate cancer. AN - 78396767; 3409448 AB - Twenty-two orchiectomized men with progressive stage D2 prostate cancer were treated with a 3-week cycle of estramustine phosphate (EMP: from day 3 to day 21) and androgen priming (from day 1 to day 4). A partial response according to the NPCP-USA criteria was shown in 4 of 20 evaluable patients. Median progression-free survival of all patients was 24 weeks (range, 4-48) and median survival, 42 weeks (range, 4-112). Although in two cases treatment had to be stopped due to a marked increase in bone pain, no life-threatening side effects were observed. The androgen sensitivity of tumors was supported by the occurrence of increase in prostatic phosphatase and in bone pain in most patients. In this group of patients, androgen priming did not seem to potentiate the effectiveness of EMP, our results being comparable to those previously reported using EMP alone. JF - Cancer chemotherapy and pharmacology AU - Boccardo, F AU - Decensi, A AU - Guarneri, D AU - Martorana, G AU - Giberti, C AU - Giuliani, L AD - Department of Clinical Oncology, National Cancer Institute, Genova, Italy. Y1 - 1988 PY - 1988 DA - 1988 SP - 172 EP - 174 VL - 22 IS - 2 SN - 0344-5704, 0344-5704 KW - Nitrogen Mustard Compounds KW - 0 KW - Estramustine KW - 35LT29625A KW - Fluoxymesterone KW - 9JU12S4YFY KW - Index Medicus KW - Drug Interactions KW - Neoplasm Staging KW - Humans KW - Aged KW - Middle Aged KW - Male KW - Orchiectomy KW - Prostatic Neoplasms -- pathology KW - Nitrogen Mustard Compounds -- therapeutic use KW - Prostatic Neoplasms -- drug therapy KW - Fluoxymesterone -- therapeutic use KW - Estramustine -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78396767?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+chemotherapy+and+pharmacology&rft.atitle=Estramustine+phosphate+%28estracyt%29+following+androgens+in+men+with+refractory+stage+D2+prostate+cancer.&rft.au=Boccardo%2C+F%3BDecensi%2C+A%3BGuarneri%2C+D%3BMartorana%2C+G%3BGiberti%2C+C%3BGiuliani%2C+L&rft.aulast=Boccardo&rft.aufirst=F&rft.date=1988-01-01&rft.volume=22&rft.issue=2&rft.spage=172&rft.isbn=&rft.btitle=&rft.title=Cancer+chemotherapy+and+pharmacology&rft.issn=03445704&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-29 N1 - Date created - 1988-09-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Modulation of induced resistance to adriamycin in two human breast cancer cell lines with tamoxifen or perhexiline maleate. AN - 78396716; 3409446 AB - The clinical utility of adriamycin in the treatment of patients with metastatic breast cancer is often-limited by the development of drug resistance. It has been recognized that in addition to the development of primary resistance against adriamycin, malignant cells can simultaneously develop cross-resistance to other agents. An adriamycin-resistant human breast cancer cell line (MCF 7Ad) was developed by exposing the parent line (MCF 7) to gradually increasing concentrations of adriamycin while the cells were being grown in monolayer. Using these lines in a clonogenic assay, the relative drug sensitivities to adriamycin, vinblastine, melphalan, 5-fluorouracil and methotrexate were studied. MCF 7Ad was 12.5-fold more resistant to adriamycin than MCF 7 and 500-fold cross-resistant to vinblastine. There was no cross-resistance to melphalan, 5-fluorouracil or methotrexate. The resistance of MCF 7Ad was decreased by simultaneous exposure to tamoxifen (by a factor of 3.33) or perhexiline maleate (by a factor of 7.50). This decreased resistance was evidenced by a shift to the left of the sensitivity curves. However, there was no consistent change in the sensitivity curves of MCF 7. At the selected concentration of tamoxifen and perhexiline maleate, the cloning efficiency of MCF 7 and MCF 7Ad was 80%-90% of control values in medium without tamoxifen, perhexiline maleate or cytotoxic drugs. The resistance of MCF 7Ad to adriamycin was associated with a lower accumulation of [14C]adriamycin than exhibited by the sensitive MCF 7 line. There was no consistent change in [14C]adriamycin accumulation in MCF 7 or MCF 7Ad when tamoxifen was added, but when perhexiline maleate was added the [14C] accumulation increased. These results suggest that the tamoxifen-induced change in MCF 7Ad adriamycin resistance was not due to an increase in the amount of cell-associated adriamycin, but rather to some other mechanism that increased the cytotoxicity of the adriamycin. JF - Cancer chemotherapy and pharmacology AU - Foster, B J AU - Grotzinger, K R AU - McKoy, W M AU - Rubinstein, L V AU - Hamilton, T C AD - Investigational Drug Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 147 EP - 152 VL - 22 IS - 2 SN - 0344-5704, 0344-5704 KW - Tamoxifen KW - 094ZI81Y45 KW - Vinblastine KW - 5V9KLZ54CY KW - Doxorubicin KW - 80168379AG KW - perhexiline maleate KW - K7V8Y90G0H KW - Perhexiline KW - KU65374X44 KW - Melphalan KW - Q41OR9510P KW - Fluorouracil KW - U3P01618RT KW - Methotrexate KW - YL5FZ2Y5U1 KW - Index Medicus KW - Vinblastine -- therapeutic use KW - Fluorouracil -- therapeutic use KW - Drug Interactions KW - Humans KW - Cell Line -- drug effects KW - Methotrexate -- therapeutic use KW - Drug Resistance KW - Melphalan -- therapeutic use KW - Tumor Stem Cell Assay KW - Breast Neoplasms -- drug therapy KW - Tamoxifen -- pharmacology KW - Doxorubicin -- pharmacokinetics KW - Perhexiline -- pharmacology KW - Perhexiline -- analogs & derivatives KW - Doxorubicin -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78396716?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+chemotherapy+and+pharmacology&rft.atitle=Modulation+of+induced+resistance+to+adriamycin+in+two+human+breast+cancer+cell+lines+with+tamoxifen+or+perhexiline+maleate.&rft.au=Foster%2C+B+J%3BGrotzinger%2C+K+R%3BMcKoy%2C+W+M%3BRubinstein%2C+L+V%3BHamilton%2C+T+C&rft.aulast=Foster&rft.aufirst=B&rft.date=1988-01-01&rft.volume=22&rft.issue=2&rft.spage=147&rft.isbn=&rft.btitle=&rft.title=Cancer+chemotherapy+and+pharmacology&rft.issn=03445704&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-29 N1 - Date created - 1988-09-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Drug solubilizers to aid pharmacologists: amorphous cyclodextrin derivatives. AN - 78370445; 2841549 AB - Conversion of crystalline alpha-, beta-, or gamma-cyclodextrins into amorphous mixtures of water soluble derivatives yields non-toxic solubilizers which dissolve drugs through the formation of inclusion complexes. From these types of compounds 2-hydroxypropyl ethers of cyclodextrins have presently been investigated and the ranges for the safe use in working with (a) receptor binding assays on membrane preparations, (b) cells in vitro, and (c) parenteral use in mice were established for these compounds. The drugs which were investigated were dissolved in amounts linearly proportionate to the concentration of the solubilizers used and did not precipitate upon dilution by aqueous media. These solubilizers may considerably facilitate pharmacological evaluation of new, water insoluble potential drugs. JF - Life sciences AU - Pitha, J AU - Irie, T AU - Sklar, P B AU - Nye, J S AD - National Institute on Aging/GRC, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 493 EP - 502 VL - 43 IS - 6 SN - 0024-3205, 0024-3205 KW - Cyclodextrins KW - 0 KW - Dextrins KW - Receptors, Adrenergic, beta KW - Starch KW - 9005-25-8 KW - Pindolol KW - BJ4HF6IU1D KW - Adenylyl Cyclases KW - EC 4.6.1.1 KW - Index Medicus KW - Rats KW - Receptors, Adrenergic, beta -- metabolism KW - Pindolol -- metabolism KW - Fibroblasts -- drug effects KW - Animals KW - Olfactory Mucosa -- enzymology KW - Solubility KW - Chemistry, Pharmaceutical KW - Humans KW - Anura KW - Adenylyl Cyclases -- metabolism KW - Receptors, Adrenergic, beta -- drug effects KW - Mice KW - Dextrins -- pharmacology KW - Cyclodextrins -- pharmacology KW - Starch -- pharmacology KW - Starch -- toxicity KW - Cyclodextrins -- toxicity KW - Dextrins -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78370445?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Drug+solubilizers+to+aid+pharmacologists%3A+amorphous+cyclodextrin+derivatives.&rft.au=Pitha%2C+J%3BIrie%2C+T%3BSklar%2C+P+B%3BNye%2C+J+S&rft.aulast=Pitha&rft.aufirst=J&rft.date=1988-01-01&rft.volume=43&rft.issue=6&rft.spage=493&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-12 N1 - Date created - 1988-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Needle sharing among intravenous drug abusers: an overview. AN - 78362686; 3136335 JF - NIDA research monograph AU - Battjes, R J AU - Pickens, R W AD - Division of Clinical Research, National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 6 VL - 80 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - AIDS/HIV KW - Injections, Intravenous KW - Humans KW - Needles KW - Acquired Immunodeficiency Syndrome -- transmission KW - Substance-Related Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78362686?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Needle+sharing+among+intravenous+drug+abusers%3A+an+overview.&rft.au=Battjes%2C+R+J%3BPickens%2C+R+W&rft.aulast=Battjes&rft.aufirst=R&rft.date=1988-01-01&rft.volume=80&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-15 N1 - Date created - 1988-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Biological evaluation of compounds for their physical dependence potential and abuse liability. XI. Drug testing program of the Committee on Problems of Drug Dependence, Inc. (1987). AN - 78354224; 2900468 JF - NIDA research monograph AU - Jacobson, A AD - Drug Design and Synthesis Section, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 466 EP - 484 VL - 81 SN - 1046-9516, 1046-9516 KW - Analgesics KW - 0 KW - Central Nervous System Depressants KW - Central Nervous System Stimulants KW - Index Medicus KW - Animals KW - Central Nervous System Depressants -- adverse effects KW - Humans KW - Central Nervous System Stimulants -- adverse effects KW - Analgesics -- adverse effects KW - Research Design -- methods KW - Substance-Related Disorders -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78354224?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Biological+evaluation+of+compounds+for+their+physical+dependence+potential+and+abuse+liability.+XI.+Drug+testing+program+of+the+Committee+on+Problems+of+Drug+Dependence%2C+Inc.+%281987%29.&rft.au=Jacobson%2C+A&rft.aulast=Jacobson&rft.aufirst=A&rft.date=1988-01-01&rft.volume=81&rft.issue=&rft.spage=466&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-15 N1 - Date created - 1988-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Initiatives at the National Institute on Drug Abuse. AN - 78353276; 3136350 JF - NIDA research monograph AU - Schuster, C AD - National Institute on Drug Abuse, Rockville, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 5 VL - 81 SN - 1046-9516, 1046-9516 KW - Nitrosamines KW - 0 KW - N-nitrosoiminodiacetic acid KW - 25081-31-6 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - United States KW - Humans KW - Adult KW - Substance-Related Disorders UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78353276?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Initiatives+at+the+National+Institute+on+Drug+Abuse.&rft.au=Schuster%2C+C&rft.aulast=Schuster&rft.aufirst=C&rft.date=1988-01-01&rft.volume=81&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-15 N1 - Date created - 1988-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - HIV infection among intravenous drug abusers in the United States and Europe. AN - 78352329; 3136347 AB - AIDS is a serious public health problem for IVDAs in the United States and Europe. Although AIDS and HIV infection are concentrated in New York and New Jersey in the United States, and in Italy and Spain in Europe, AIDS has been diagnosed and reported among IVDAs in all 50 States of the United States and 16 countries in Europe. It is quite apparent that, once HIV is introduced into a group of IVDAs, it can spread readily between IVDAs, to their sexual partners, and to their children in utero. Unfortunately, one can only expect HIV seroprevalence rates and AIDS cases to continue to increase among IVDAs worldwide for at least the next several years. Concerted efforts to develop, implement, and evaluate potential prevention strategies among IVDAs are urgently needed. JF - NIDA research monograph AU - Haverkos, H W AD - Division of Clinical Research, National Institute on Drug Abuse, Rockville, MD 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 7 EP - 17 VL - 80 SN - 1046-9516, 1046-9516 KW - Index Medicus KW - AIDS/HIV KW - United States KW - Injections, Intravenous KW - Humans KW - Europe KW - Needles KW - Acquired Immunodeficiency Syndrome -- transmission KW - Substance-Related Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78352329?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=HIV+infection+among+intravenous+drug+abusers+in+the+United+States+and+Europe.&rft.au=Haverkos%2C+H+W&rft.aulast=Haverkos&rft.aufirst=H&rft.date=1988-01-01&rft.volume=80&rft.issue=&rft.spage=7&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-15 N1 - Date created - 1988-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Signals controlling alternative splicing of major histocompatibility complex H-2 class I pre-mRNA. AN - 78340248; 3397132 AB - The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein: the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 bp from the splice junction and is contained in the sequence AGTGATGG. D-region genes, which use only the downstream splice site, have the sequence AGTGGTGG. We have used in vitro mutagenesis to change this A of the H-2Kb gene to G and have made the reciprocal change in H-2Dd. Elimination of this adenosine in H-2Kb alters the pattern of pre-mRNA splicing and results in a predominance of the Kb molecules with short exon 8 encoded sequences. However, the addition of an adenosine in H-2Dd is not sufficient to direct splicing to the upstream site. JF - Immunogenetics AU - Handy, D E AU - McCluskey, J AU - Lew, A M AU - Coligan, J E AU - Margulies, D H AD - Biological Resources Branch, National Institutes of Health, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 81 EP - 90 VL - 28 IS - 2 SN - 0093-7711, 0093-7711 KW - H-2 Antigens KW - 0 KW - Nucleic Acid Precursors KW - RNA, Messenger KW - Index Medicus KW - L Cells (Cell Line) KW - Animals KW - Base Sequence KW - Cell Membrane -- immunology KW - Transfection KW - DNA Mutational Analysis KW - Introns KW - Mice KW - Gene Expression Regulation KW - Nucleic Acid Precursors -- genetics KW - Genes, MHC Class I KW - H-2 Antigens -- genetics KW - RNA Splicing KW - RNA, Messenger -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78340248?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Association+for+Vascular+Access&rft.atitle=Defining+the+Specialty+of+Vascular+Access+through+Consensus%3A+Shaping+the+Future+of+Vascular+Access&rft.au=Davis%2C+Lois%3BOwens%2C+Andrea+K%3BThompson%2C+Judy&rft.aulast=Davis&rft.aufirst=Lois&rft.date=2016-09-01&rft.volume=21&rft.issue=3&rft.spage=125&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Association+for+Vascular+Access&rft.issn=15528855&rft_id=info:doi/10.1016%2Fj.java.2016.06.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-07 N1 - Date created - 1988-09-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Increased metallothionein gene expression in 5-aza-2'-deoxycytidine-induced resistance to cadmium cytotoxicity. AN - 78339854; 2456160 AB - The pyrimidine analog, 5-azacytidine (AZA-CR), has been shown to increase the expression of the metallothionein (MT) gene and to induce tolerance to cadmium toxicity. Since incorporation into DNA of AZA-CR appears to be required for this effect, the deoxynucleoside of AZA-CR should also be effective. Therefore, this study was undertaken to assess the effect of 5-aza-2'-deoxycytidine (AZA-CdR) pretreatment on cadmium-induced cytotoxicity and MT expression in cultured cells. TRL 1215 cells in log phase of growth were exposed to AZA-CdR (0.4, 0.8, 4.0, 8.0 microM) followed 48 h later by the addition of cadmium (10 microM). MT concentrations were measured 24 h after the addition of cadmium. AZA-CdR alone caused modest, dose-related increases in MT levels (2.3-fold maximum), while cadmium alone resulted in a 9.5-fold increase. Pretreatment with AZA-CdR in combination with cadmium caused a 19--24-fold increase in cellular MT at all doses of AZA-CdR. Addition of the DNA synthesis inhibitor, hydroxyurea (HU), to the incubation medium during AZA-CdR exposure prevented the enhancing effect of the analog on cadmium induction of MT accumulation. Time course studies revealed that AZA-CdR pretreatment reduced the time required for cadmium to induce MT levels from 4--8 h to 0--2 h. AZA-CdR pretreated cells placed in suspension with cadmium (125 microM) showed a marked reduction in cadmium-induced cytotoxicity as reflected by reduced glutamic-oxaloacetic transaminase (GOT) loss. Uptake studies showed that AZA-CdR pretreatment had no effect on cadmium transport during the initial phases of exposure, indicating that an alteration in the toxicokinetics of the metal did not account for the reduction in toxicity. AZA-CdR did, however, cause hypomethylation of the MT-I gene. These results suggest that AZA-CdR pretreatment induces tolerance to cadmium toxicity by increasing the genetic expression of MT possibly through hypomethylation of the MT gene. JF - Chemico-biological interactions AU - Waalkes, M P AU - Miller, M S AU - Wilson, M J AU - Bare, R M AU - McDowell, A E AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick, MD 21701-1013. Y1 - 1988 PY - 1988 DA - 1988 SP - 189 EP - 204 VL - 66 IS - 3-4 SN - 0009-2797, 0009-2797 KW - Cadmium KW - 00BH33GNGH KW - decitabine KW - 776B62CQ27 KW - DNA KW - 9007-49-2 KW - Metallothionein KW - 9038-94-2 KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Azacitidine KW - M801H13NRU KW - Hydroxyurea KW - X6Q56QN5QC KW - Index Medicus KW - Animals KW - Azacitidine -- pharmacology KW - Dose-Response Relationship, Drug KW - Azacitidine -- analogs & derivatives KW - Drug Resistance KW - Nucleic Acid Hybridization KW - Rats KW - Rats, Inbred F344 KW - Cell Survival -- drug effects KW - Kinetics KW - DNA -- genetics KW - Methylation KW - Cell Line KW - Liver -- drug effects KW - Cadmium -- toxicity KW - Metallothionein -- genetics KW - Liver -- metabolism KW - Gene Expression Regulation -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78339854?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Increased+metallothionein+gene+expression+in+5-aza-2%27-deoxycytidine-induced+resistance+to+cadmium+cytotoxicity.&rft.au=Waalkes%2C+M+P%3BMiller%2C+M+S%3BWilson%2C+M+J%3BBare%2C+R+M%3BMcDowell%2C+A+E&rft.aulast=Waalkes&rft.aufirst=M&rft.date=1988-01-01&rft.volume=66&rft.issue=3-4&rft.spage=189&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-02 N1 - Date created - 1988-09-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Comparison of the effects of carbon tetrachloride and of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the disposition of linoleic acid in rat liver in vitro. AN - 78336882; 3135123 AB - Both 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and carbon tetrachloride (CCl4) have conspicuous effects on lipid metabolism in rat liver. Although it is generally accepted that CCl4 administration leads to hepatic lipid peroxidation in vivo, conflicting reports from different laboratories make it unclear whether or not lipid peroxidation is involved in the mechanism of toxicity of TCDD. The present study involved pretreating F344 rats with CCl4 or TCDD, then at predetermined times thereafter, giving [U-14C]linoleic acid. A variety of compound classes were monitored in extracts of liver taken 30 min after the label was given. A previously unreported effect of CCl4 was a conspicuous increase in turnover of 1,2-diglycerides. That CCl4 did cause lipid peroxidation was evident from the presence of allylic hydroxyacids not seen in vehicle-treated controls, greatly increased radioactivity in protein-bound material, and decreased levels of arachidonate without decreased synthesis from linolate. Where effects of TCDD pretreatment could be seen, they were much less than the corresponding effects of CCl4. No allylic hydroxyacids were detected in livers of TCDD-treated rats. The concentration of arachidonate was not reduced, and elongation of linolate was not stimulated, indicating that TCDD did not cause extensive-but-repaired peroxidation. It is concluded that while TCDD may slightly increase hepatic lipid peroxidation in rats in vivo, the extent of such stimulation appears to be too slight to account for the toxicity of TCDD. JF - Chemico-biological interactions AU - Albro, P W AU - Corbett, J T AU - Schroeder, J L AU - Harvan, D AD - Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 267 EP - 285 VL - 66 IS - 3-4 SN - 0009-2797, 0009-2797 KW - Aldehydes KW - 0 KW - Arachidonic Acids KW - Carbon Radioisotopes KW - Dioxins KW - Fatty Acids KW - Linoleic Acids KW - Lipid Peroxides KW - Polychlorinated Dibenzodioxins KW - Arachidonic Acid KW - 27YG812J1I KW - Linoleic Acid KW - 9KJL21T0QJ KW - Carbon Tetrachloride KW - CL2T97X0V0 KW - 4-hydroxy-2-nonenal KW - K1CVM13F96 KW - Index Medicus KW - Animals KW - Arachidonic Acids -- metabolism KW - Fatty Acids -- metabolism KW - Rats KW - Oxidation-Reduction KW - Rats, Inbred F344 KW - Kinetics KW - Aldehydes -- metabolism KW - Gas Chromatography-Mass Spectrometry KW - Chromatography, Thin Layer KW - Lipid Peroxides -- metabolism KW - Female KW - Carbon Tetrachloride -- pharmacology KW - Liver -- drug effects KW - Polychlorinated Dibenzodioxins -- pharmacology KW - Linoleic Acids -- metabolism KW - Liver -- metabolism KW - Dioxins -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78336882?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Comparison+of+the+effects+of+carbon+tetrachloride+and+of+2%2C3%2C7%2C8-tetrachlorodibenzo-p-dioxin+on+the+disposition+of+linoleic+acid+in+rat+liver+in+vitro.&rft.au=Albro%2C+P+W%3BCorbett%2C+J+T%3BSchroeder%2C+J+L%3BHarvan%2C+D&rft.aulast=Albro&rft.aufirst=P&rft.date=1988-01-01&rft.volume=21&rft.issue=3&rft.spage=140&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Association+for+Vascular+Access&rft.issn=15528855&rft_id=info:doi/10.1016%2Fj.java.2016.06.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-02 N1 - Date created - 1988-09-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Ex vivo activation of killer monocytes (AKM) and their application to the treatment of human cancer. AN - 78336451; 3135291 AB - Human blood monocytes activated by gamma interferon have been shown to be highly tumoricidal against colon cancer cells in vitro. Monocytes from patients with peritoneal colorectal carcinomatosis (PCC) were purified by a combination of cytapheresis and elutriation procedures, followed by in vitro incubation with gamma interferon for 18 hours. After debulking surgery, activated killer monocytes (AKM) were reinfused into patients' peritoneal cavities weekly for 16 weeks. To date, six patients have completed the entire protocol and three have completed maintenance therapy. All have tolerated the therapy well with acceptable toxicity. Midway through the protocol, we analyzed the trafficking pattern of the AKM by prelabeling them with 111In. Distribution was relatively homogeneous throughout the peritoneum; at the second staging celiotomy, three of the seven PCC patients were found to have very small amounts of recurrent disease in places to which the AKM were felt to have had limited access (other areas remained disease-free); these areas of recurrent disease were surgically resectable. AKM have also been infused systemically into five cancer patients. First, the patients were infused with unactivated 111In-labeled monocytes; 1 month later each patient received 111In-labeled gamma interferon-activated AKM. Trafficking studies indicated that both forms of monocytes migrated to sites in the reticuloendothelial system. We have seen virtually no complications from intravenous infusions of either unactivated or gamma interferon-activated AKM.(ABSTRACT TRUNCATED AT 250 WORDS) JF - Journal of clinical apheresis AU - Stevenson, H C AU - Lacerna, L V AU - Sugarbaker, P H AD - Biological Response Modifiers Program, NCI-Frederick Cancer Research Facility, MD 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 118 EP - 121 VL - 4 IS - 2-3 SN - 0733-2459, 0733-2459 KW - Interferon-gamma KW - 82115-62-6 KW - Index Medicus KW - Humans KW - Adult KW - Clinical Trials as Topic KW - Monocytes -- drug effects KW - Interferon-gamma -- pharmacology KW - Middle Aged KW - Male KW - Female KW - Lymphocyte Activation KW - Peritoneal Neoplasms -- secondary KW - Immunization, Passive KW - Leukocytes, Mononuclear -- immunology KW - Leukocytes, Mononuclear -- drug effects KW - Peritoneal Neoplasms -- therapy KW - Killer Cells, Natural -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78336451?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+apheresis&rft.atitle=Ex+vivo+activation+of+killer+monocytes+%28AKM%29+and+their+application+to+the+treatment+of+human+cancer.&rft.au=Stevenson%2C+H+C%3BLacerna%2C+L+V%3BSugarbaker%2C+P+H&rft.aulast=Stevenson&rft.aufirst=H&rft.date=1988-01-01&rft.volume=4&rft.issue=2-3&rft.spage=118&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+apheresis&rft.issn=07332459&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-02 N1 - Date created - 1988-09-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: J Clin Apheresis 1989;5(1):59 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Seizure-induced alterations in the metabolism of hippocampal opioid peptides suggest opioid modulation of seizure-related behaviors. AN - 78335094; 2899842 AB - The evidence accumulated so far indicates that seizure activity exerts profound changes on the metabolism of opioid peptides in the hippocampus. Our data consistently show a large transient decrease in dynorphin and a modest decrease in enkephalin in the hippocampus following either a single ECS or KA injection. These initial reductions, which are indicative of increased release, may trigger the biosynthetic process of hippocampal opioids and result in an overproduction of the peptides seen in the rebound phase. However, the amount and timing of the rebound in enkephalin and dynorphin levels in response to repeated ECS, amygdaloid kindling, or KA differ drastically: a rapid and sustained increase in ME-LI follows all three treatments, in contrast to a slow recovery after a large and sustained decrease in DN-LI induced by repeated ECS and amygdaloid kindling. These results, which are unique to the hippocampus, suggest that differential mechanisms are operative in regulating the metabolism of these two opioid peptides in the hippocampus. It is likely that a well-coordinated regulation of hippocampal function can be achieved through the differential release of enkephalin and dynorphin and their subsequent interactions at different subtypes of opioid receptors following seizure activities. From a functional point of view, our data provide a neurochemical correlate of previous reports that brain opioid peptides may mediate ECS-induced behavioral alterations, such as changes in seizure threshold, postictal depression, and retrograde amnesia. The robust changes in the levels of opioid peptides in kindled rats, plus shortening of the kindling process by pretreatment with mu opioid antagonists, strongly suggest the involvement of brain opioid peptides in the development of kindling. Finally, these studies show clear evidence that enkephalin in the hippocampus is important in KA-induced WDS, a component of the opiate withdrawal syndrome in rodents (Isaacson and Lanthorn 1981). Further studies should help distinguish the regulatory mechanisms responsible for changes in opioid peptide metabolism during states of hyperexcitability in the hippocampal formation. JF - NIDA research monograph AU - Hong, J S AU - McGinty, J F AU - Grimes, L AU - Kanamatsu, T AU - Obie, J AU - Mitchell, C L AD - Laboratory of Behavioral and Neurological Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 48 EP - 66 VL - 82 SN - 1046-9516, 1046-9516 KW - Endorphins KW - 0 KW - Enkephalin, Methionine KW - 58569-55-4 KW - Dynorphins KW - 74913-18-1 KW - Kainic Acid KW - SIV03811UC KW - Colchicine KW - SML2Y3J35T KW - Index Medicus KW - Animals KW - Amygdala -- metabolism KW - Kindling, Neurologic KW - Enkephalin, Methionine -- metabolism KW - Electroshock KW - Dynorphins -- metabolism KW - Seizures -- chemically induced KW - Endorphins -- metabolism KW - Hippocampus -- metabolism KW - Seizures -- metabolism KW - Behavior, Animal UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78335094?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NIDA+research+monograph&rft.atitle=Seizure-induced+alterations+in+the+metabolism+of+hippocampal+opioid+peptides+suggest+opioid+modulation+of+seizure-related+behaviors.&rft.au=Hong%2C+J+S%3BMcGinty%2C+J+F%3BGrimes%2C+L%3BKanamatsu%2C+T%3BObie%2C+J%3BMitchell%2C+C+L&rft.aulast=Hong&rft.aufirst=J&rft.date=1988-01-01&rft.volume=82&rft.issue=&rft.spage=48&rft.isbn=&rft.btitle=&rft.title=NIDA+research+monograph&rft.issn=10469516&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-02 N1 - Date created - 1988-09-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Human myeloperoxidase and thyroid peroxidase, two enzymes with separate and distinct physiological functions, are evolutionarily related members of the same gene family. AN - 78333508; 2840655 AB - Human myeloperoxidase and human thyroid peroxidase nucleotide and amino acid sequences were compared. The global similarities of the nucleotide and amino acid sequences are 46% and 44%, respectively. These similarities are most evident within the coding sequence, especially that encoding the myeloperoxidase functional subunits. These results clearly indicate that myeloperoxidase and thyroid peroxidase are members of the same gene family and diverged from a common ancestral gene. The residues at 416 in myeloperoxidase and 407 in thyroid peroxidase were estimated as possible candidates for the proximal histidine residues that link to the iron centers of the enzymes. The primary structures around these histidine residues were compared with those of other known peroxidases. The similarity in this region between the two animal peroxidases (amino acid 396-418 in thyroid peroxidase and 405-427 in myeloperoxidase) is 74%; however, those between the animal peroxidases and other yeast and plant peroxidases are not significantly high, although several conserved features have been observed. The possible location of the distal histidine residues in myeloperoxidase and thyroid peroxidase amino acid sequences are also discussed. JF - Proteins AU - Kimura, S AU - Ikeda-Saito, M AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 113 EP - 120 VL - 3 IS - 2 SN - 0887-3585, 0887-3585 KW - Peroxidase KW - EC 1.11.1.7 KW - Iodide Peroxidase KW - EC 1.11.1.8 KW - Index Medicus KW - Base Sequence KW - Sequence Homology, Nucleic Acid KW - Humans KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Genes KW - Peroxidase -- genetics KW - Biological Evolution KW - Iodide Peroxidase -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78333508?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proteins&rft.atitle=Human+myeloperoxidase+and+thyroid+peroxidase%2C+two+enzymes+with+separate+and+distinct+physiological+functions%2C+are+evolutionarily+related+members+of+the+same+gene+family.&rft.au=Kimura%2C+S%3BIkeda-Saito%2C+M&rft.aulast=Kimura&rft.aufirst=S&rft.date=1988-01-01&rft.volume=3&rft.issue=2&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Proteins&rft.issn=08873585&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-26 N1 - Date created - 1988-08-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Kaposi's sarcoma and nitrite inhalants. AN - 78332187; 2899948 JF - Advances in biochemical psychopharmacology AU - Haverkos, H W AD - Clinical Medicine Branch, National Institute on Drug Abuse, Alcohol, Drug Abuse, Rockville, Maryland 20857. Y1 - 1988 PY - 1988 DA - 1988 SP - 165 EP - 172 VL - 44 SN - 0065-2229, 0065-2229 KW - Amyl Nitrite KW - 8017-89-8 KW - Index Medicus KW - AIDS/HIV KW - Risk Factors KW - Humans KW - Homosexuality KW - Administration, Inhalation KW - Male KW - Acquired Immunodeficiency Syndrome -- complications KW - Amyl Nitrite -- adverse effects KW - Sarcoma, Kaposi -- chemically induced KW - Skin Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78332187?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+biochemical+psychopharmacology&rft.atitle=Kaposi%27s+sarcoma+and+nitrite+inhalants.&rft.au=Haverkos%2C+H+W&rft.aulast=Haverkos&rft.aufirst=H&rft.date=1988-01-01&rft.volume=19&rft.issue=1&rft.spage=35&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Association+for+Vascular+Access&rft.issn=15528855&rft_id=info:doi/10.1016%2Fj.java.2013.11.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-08 N1 - Date created - 1988-09-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - raf protooncogene expression in neural and immune tissues. AN - 78328285; 3041752 JF - Advances in biochemical psychopharmacology AU - Mark, G E AU - Pfeifer, A AU - Mann, D L AU - Harris, C C AU - Berman, R AU - Pert, C B AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 45 EP - 55 VL - 44 SN - 0065-2229, 0065-2229 KW - Proto-Oncogene Proteins KW - 0 KW - Proto-Oncogene Proteins c-raf KW - EC 2.7.11.1 KW - Index Medicus KW - Animals KW - Brain Neoplasms -- genetics KW - Humans KW - B-Lymphocytes -- immunology KW - T-Lymphocytes -- immunology KW - Cloning, Molecular KW - Gene Expression Regulation KW - Proto-Oncogenes KW - Proto-Oncogene Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78328285?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+biochemical+psychopharmacology&rft.atitle=raf+protooncogene+expression+in+neural+and+immune+tissues.&rft.au=Mark%2C+G+E%3BPfeifer%2C+A%3BMann%2C+D+L%3BHarris%2C+C+C%3BBerman%2C+R%3BPert%2C+C+B&rft.aulast=Mark&rft.aufirst=G&rft.date=1988-01-01&rft.volume=44&rft.issue=&rft.spage=45&rft.isbn=&rft.btitle=&rft.title=Advances+in+biochemical+psychopharmacology&rft.issn=00652229&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-09-08 N1 - Date created - 1988-09-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Inhalation toxicity and carcinogenesis studies of methylene chloride (dichloromethane) in F344/N rats and B6C3F1 mice. AN - 78300992; 3389664 JF - Annals of the New York Academy of Sciences AU - Mennear, J H AU - McConnell, E E AU - Huff, J E AU - Renne, R A AU - Giddens, E AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 343 EP - 351 VL - 534 SN - 0077-8923, 0077-8923 KW - Hydrocarbons, Chlorinated KW - 0 KW - Methylene Chloride KW - 588X2YUY0A KW - Index Medicus KW - Rats KW - Mammary Neoplasms, Experimental -- chemically induced KW - Animals KW - Rats, Inbred F344 KW - Mice KW - Administration, Inhalation KW - Male KW - Female KW - Hydrocarbons, Chlorinated -- toxicity KW - Methylene Chloride -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300992?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Inhalation+toxicity+and+carcinogenesis+studies+of+methylene+chloride+%28dichloromethane%29+in+F344%2FN+rats+and+B6C3F1+mice.&rft.au=Mennear%2C+J+H%3BMcConnell%2C+E+E%3BHuff%2C+J+E%3BRenne%2C+R+A%3BGiddens%2C+E&rft.aulast=Mennear&rft.aufirst=J&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=343&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Laboratory animal toxicity and carcinogenesis testing. Underlying concepts, advantages and constraints. AN - 78300834; 3291727 JF - Annals of the New York Academy of Sciences AU - Rall, D P AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 78 EP - 83 VL - 534 SN - 0077-8923, 0077-8923 KW - Index Medicus KW - Animals KW - Mutagenicity Tests KW - Humans KW - Disease Models, Animal KW - Animals, Laboratory KW - Neoplasms, Experimental -- chemically induced KW - Toxicology -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Association+for+Vascular+Access&rft.atitle=Development+and+Testing+of+a+Short+Peripheral+Intravenous+Catheter+Insertion+Skills+Checklist&rft.au=Schuster%2C+Catherine%3BStahl%2C+Brian%3BMurray%2C+Connie%3BKeleekai%2C+Nowai+L%3BGlover%2C+Kevin&rft.aulast=Schuster&rft.aufirst=Catherine&rft.date=2016-12-01&rft.volume=21&rft.issue=4&rft.spage=196&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Association+for+Vascular+Access&rft.issn=15528855&rft_id=info:doi/10.1016%2Fj.java.2016.08.003 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Chronic studies in rodents of vinyl acetate and compounds related to acrolein. AN - 78300709; 3389658 JF - Annals of the New York Academy of Sciences AU - Lijinsky, W AD - NCI-Frederick Cancer Research Facility, LBI-Basic Research Program, Maryland 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 246 EP - 254 VL - 534 SN - 0077-8923, 0077-8923 KW - Aldehydes KW - 0 KW - Propanols KW - Vinyl Compounds KW - allyl alcohol KW - 3W678R12M0 KW - Acrolein KW - 7864XYD3JJ KW - 1-Propanol KW - 96F264O9SV KW - vinyl acetate KW - L9MK238N77 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Biotransformation KW - 1-Propanol -- toxicity KW - Lethal Dose 50 KW - Vinyl Compounds -- toxicity KW - Acrolein -- analogs & derivatives KW - Acrolein -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300709?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nephron&rft.atitle=Renal+Association+Clinical+Practice+Guideline+on+Vascular+Access+for+Haemodialysis&rft.au=Fluck%2C+Richard%3BKumwenda%2C+Mick&rft.aulast=Fluck&rft.aufirst=Richard&rft.date=2011-05-01&rft.volume=118&rft.issue=&rft.spage=c225&rft.isbn=&rft.btitle=&rft.title=Nephron&rft.issn=00282766&rft_id=info:doi/10.1159%2F000328071 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Relationships between in vitro genetic toxicity and carcinogenicity studies in animals. AN - 78300661; 3291706 AB - In vitro genetic toxicity assays currently in use cannot be used to unambiguously define all potential carcinogens. The relationship between the various in vitro endpoints (mutation, cytogenetic effects, transformation) and the patterns of tumorigenicity is quite complex. Additional data, particularly for noncarcinogens, are needed to better understand the inconsistencies and to define the limits of predictability. For example, we must empirically define the concordance and discordance between in vivo and in vitro systems related to chemical classes or structures. At this time, since there are no nontrivial generalizations and no general predictivity is possible, we must use judgment in applying short-term test results. There is a clear need to identify as soon as possible the minimum number of in vitro systems that can be used, nonredundantly, to discriminate between carcinogens and noncarcinogens. However, we must continue to address the sources of discordance in order to understand the ways in which short-term test results can and cannot be used. Many trans-sex/trans-species carcinogens show the capacity to induce multiple endpoints of genetic toxicity and this class of rodent carcinogens may have the greatest implications for potential human health effects. A much more selective use of short-term test results to identify potential carcinogens such as these will achieve many of the goals for which these tests were developed. JF - Annals of the New York Academy of Sciences AU - Tennant, R W AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 127 EP - 132 VL - 534 SN - 0077-8923, 0077-8923 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Animals KW - In Vitro Techniques KW - Mutagenicity Tests -- methods KW - DNA -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300661?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Relationships+between+in+vitro+genetic+toxicity+and+carcinogenicity+studies+in+animals.&rft.au=Tennant%2C+R+W&rft.aulast=Tennant&rft.aufirst=R&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=127&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Chronic toxicity results and ongoing studies of 1,3-butadiene by the National Toxicology Program. AN - 78300610; 3291722 JF - Annals of the New York Academy of Sciences AU - Melnick, R L AU - Huff, J E AU - Haseman, J K AU - McConnell, E E AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 648 EP - 662 VL - 534 SN - 0077-8923, 0077-8923 KW - Butadienes KW - 0 KW - 1,3-butadiene KW - JSD5FGP5VD KW - Index Medicus KW - Animals KW - Hemangiosarcoma -- chemically induced KW - Mice KW - Rats, Inbred Strains KW - Body Weight KW - Rats KW - Heart Neoplasms -- chemically induced KW - Heart Neoplasms -- pathology KW - Hemangiosarcoma -- pathology KW - Follow-Up Studies KW - Administration, Inhalation KW - Time Factors KW - Female KW - Male KW - Butadienes -- toxicity KW - Butadienes -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300610?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Chronic+toxicity+results+and+ongoing+studies+of+1%2C3-butadiene+by+the+National+Toxicology+Program.&rft.au=Melnick%2C+R+L%3BHuff%2C+J+E%3BHaseman%2C+J+K%3BMcConnell%2C+E+E&rft.aulast=Melnick&rft.aufirst=R&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=648&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Carcinogenesis studies: results of 398 experiments on 104 chemicals from the U.S. National Toxicology Program. AN - 78300484; 3291703 JF - Annals of the New York Academy of Sciences AU - Huff, J E AU - McConnell, E E AU - Haseman, J K AU - Boorman, G A AU - Eustis, S L AU - Schwetz, B A AU - Rao, G N AU - Jameson, C W AU - Hart, L G AU - Rall, D P AD - National Institute of Environmental Health Sciences, National Toxicology Program, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 30 VL - 534 SN - 0077-8923, 0077-8923 KW - Carcinogens KW - 0 KW - Index Medicus KW - United States KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Mutagenicity Tests KW - Environmental Exposure KW - Toxicology KW - National Health Programs KW - Male KW - Female KW - Neoplasms, Experimental -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300484?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Carcinogenesis+studies%3A+results+of+398+experiments+on+104+chemicals+from+the+U.S.+National+Toxicology+Program.&rft.au=Huff%2C+J+E%3BMcConnell%2C+E+E%3BHaseman%2C+J+K%3BBoorman%2C+G+A%3BEustis%2C+S+L%3BSchwetz%2C+B+A%3BRao%2C+G+N%3BJameson%2C+C+W%3BHart%2C+L+G%3BRall%2C+D+P&rft.aulast=Huff&rft.aufirst=J&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Epidemiologic approaches to evaluation. Discussion paper. AN - 78300183; 3389668 JF - Annals of the New York Academy of Sciences AU - Rogan, W J AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 394 VL - 534 SN - 0077-8923, 0077-8923 KW - Carcinogens KW - 0 KW - Index Medicus KW - Humans KW - Environmental Exposure KW - Epidemiologic Methods KW - Carcinogens -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78300183?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Epidemiologic+approaches+to+evaluation.+Discussion+paper.&rft.au=Rogan%2C+W+J&rft.aulast=Rogan&rft.aufirst=W&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=394&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Carcinogenesis studies of benzene, methyl benzene, and dimethyl benzenes. AN - 78299850; 3389672 JF - Annals of the New York Academy of Sciences AU - Huff, J E AU - Eastin, W AU - Roycroft, J AU - Eustis, S L AU - Haseman, J K AD - National Institute of Environmental Health Sciences, National Toxicology Program, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 427 EP - 440 VL - 534 SN - 0077-8923, 0077-8923 KW - Xylenes KW - 0 KW - Toluene KW - 3FPU23BG52 KW - Benzene KW - J64922108F KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Mutagenicity Tests KW - Male KW - Female KW - Xylenes -- toxicity KW - Benzene -- toxicity KW - Toluene -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78299850?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Carcinogenesis+studies+of+benzene%2C+methyl+benzene%2C+and+dimethyl+benzenes.&rft.au=Huff%2C+J+E%3BEastin%2C+W%3BRoycroft%2C+J%3BEustis%2C+S+L%3BHaseman%2C+J+K&rft.aulast=Huff&rft.aufirst=J&rft.date=1988-01-01&rft.volume=534&rft.issue=&rft.spage=427&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-04 N1 - Date created - 1988-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Viruses, oncogenes, and cancer. AN - 78298748; 3292041 AB - Our current theories of virus-induced cellular transformation have changed with the emerging recognition that all normal cells contain proto-oncogenes which convert to oncogenes and induce transformation when activated and/or amplified. Cellular oncogenes have been identified by homology to the transforming genes of acute retroviruses and by the transforming activity of tumor cell DNA in transfection assays. More than two dozen cellular oncogenes identified to date constitute a heterogeneous group of genes which are remarkably conserved among highly diverse species. Expression of proto-oncogenes is linked to normal growth and development; whereas their expression as oncogenes due to gene mutation, rearrangement, amplification or other processes leading to altered or overexpression is associated with the development of tumors. Functions of oncogene proteins are being identified. These include unique protein kinase activity, growth factor/growth factor receptor properties, and the presence of DNA-binding polypeptides. It also appears that cooperation between several activated cellular oncogenes may be required in the multistep process of oncogenesis. Our recent in vitro experimental evidence supports that human cell carcinogenesis is indeed a multistep process. In addition, the involvement of the activated cellular transforming genes met and H-ras in chemically induced human cell carcinogenesis has been shown. Advancement in molecular biology of oncogenes and their products is likely to result in improvements in cancer diagnosis and cancer therapy. JF - Cancer detection and prevention AU - Rhim, J S AD - Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 139 EP - 149 VL - 11 IS - 3-6 SN - 0361-090X, 0361-090X KW - Index Medicus KW - Humans KW - Gene Expression Regulation KW - Oncogenes KW - Retroviridae -- genetics KW - Neoplasms -- etiology KW - Cell Transformation, Neoplastic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78298748?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+detection+and+prevention&rft.atitle=Viruses%2C+oncogenes%2C+and+cancer.&rft.au=Rhim%2C+J+S&rft.aulast=Rhim&rft.aufirst=J&rft.date=1988-01-01&rft.volume=11&rft.issue=3-6&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=Cancer+detection+and+prevention&rft.issn=0361090X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-24 N1 - Date created - 1988-08-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Lack of cardiovascular tolerance during intravenous cocaine infusions in human volunteers. AN - 78288894; 3386392 AB - Acute tolerance to the cardiovascular effects of cocaine has been hypothesized from experiments in which the plasma concentrations of cocaine were rapidly changing. We studied the cardiovascular responses of 8 male human subjects for 4 hours following intravenous bolus doses of cocaine, and compared these to responses in the same subjects after intravenous bolus doses of cocaine followed by continuous intravenous infusions of cocaine designed to maintain steady state plasma levels of cocaine. We found little evidence of tolerance to the tachycardia and hypertensive effects of cocaine during a four hour exposure. Lack of tolerance to the cardiovascular effects of cocaine may be a factor in some types of cocaine related toxicity among cocaine abusers. JF - Life sciences AU - Kumor, K AU - Sherer, M AU - Thompson, L AU - Cone, E AU - Mahaffey, J AU - Jaffe, J H AD - National Institute on Drug Abuse, Addiction Research Center, Baltimore, MD 21224. Y1 - 1988 PY - 1988 DA - 1988 SP - 2063 EP - 2071 VL - 42 IS - 21 SN - 0024-3205, 0024-3205 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Heart Rate -- drug effects KW - Infusions, Intravenous KW - Double-Blind Method KW - Random Allocation KW - Humans KW - Adult KW - Blood Pressure -- drug effects KW - Male KW - Cocaine -- pharmacokinetics KW - Cardiovascular System -- drug effects KW - Cocaine -- blood KW - Cocaine -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78288894?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Lack+of+cardiovascular+tolerance+during+intravenous+cocaine+infusions+in+human+volunteers.&rft.au=Kumor%2C+K%3BSherer%2C+M%3BThompson%2C+L%3BCone%2C+E%3BMahaffey%2C+J%3BJaffe%2C+J+H&rft.aulast=Kumor&rft.aufirst=K&rft.date=1988-01-01&rft.volume=42&rft.issue=21&rft.spage=2063&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-07-29 N1 - Date created - 1988-07-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neoplasms of the skin and other organs observed in Swiss mice treated with nitrosoalkylureas. AN - 78281762; 3384841 AB - A number of nitrosoalkylureas, nitrosoalkylcarbamates, and chlorinated nitrosotrialkylureas were painted twice a week on the skin of female Swiss mice at a concentration of 40 mM. Of the 29 compounds, 16 induced skin tumors in 4 or more of 20 mice; 9 compounds produced tumors in 10 or more mice. Most of the skin tumors were squamous cell or basal cell carcinomas, and some sarcomas. These carcinomas and sarcomas of the skin were large, invasive, and in several animals there were multiple large metastases to the lungs and lymph nodes. Treatment with several of the compounds was associated with poor survival. The median survival in many other groups was reduced considerably below the 2-year survival of acetone-treated controls. Many of the treatments led to development of tumors of internal organs, including mammary carcinomas, adenocarcinomas and squamous cell carcinomas of the lung, and tumors of the stomach. The stomach tumors might have arisen through exposure to the compound licked from the skin. It appears that several of the compounds were absorbed through the skin of the mice and exerted their effect systemically. JF - Journal of cancer research and clinical oncology AU - Lijinsky, W AU - Reuber, M D AD - NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, MD 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 245 EP - 249 VL - 114 IS - 3 SN - 0171-5216, 0171-5216 KW - Methylnitrosourea KW - 684-93-5 KW - Index Medicus KW - Animals KW - Mice KW - Lung Neoplasms -- chemically induced KW - Female KW - Administration, Topical KW - Alkylation KW - Neoplasms, Experimental -- chemically induced KW - Skin Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78281762?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cancer+research+and+clinical+oncology&rft.atitle=Neoplasms+of+the+skin+and+other+organs+observed+in+Swiss+mice+treated+with+nitrosoalkylureas.&rft.au=Lijinsky%2C+W%3BReuber%2C+M+D&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=114&rft.issue=3&rft.spage=245&rft.isbn=&rft.btitle=&rft.title=Journal+of+cancer+research+and+clinical+oncology&rft.issn=01715216&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-02 N1 - Date created - 1988-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of feminization of male F344 rats on induction of tumors and on nucleic acid alkylation by nitrosobis-(2-oxopropyl)amine. AN - 78264909; 3383283 AB - Groups of male and female F344 rats were treated twice weekly by gavage with 2.5 mg of nitrosobis-(2-oxopropyl)amine (BOP) for 35 weeks. Additional groups given the same treatment were male rats castrated at birth, male rats bearing an implant of a pellet containing estradiol and castrated male rats bearing an estradiol pellet. Most rats died with tumors related to the treatment; intact male rats survived the least well of the five groups. Most rats in all groups had alveolar/bronchiolar neoplasms of the lung. Many of the male rats also had follicular cell neoplasms of the thyroid and transitional cell neoplasms of the urinary bladder and kidney pelvis; there were no liver tumors in intact male rats. Almost all female rats and castrated male rats had liver neoplasms, including hepatocellular, cholangiocellular and hemangiosarcomatous neoplasms, but few neoplasms of the thyroid, kidney or bladder. The male rats feminized with estradiol, intact or castrated, had liver neoplasms, mainly cholangiocellular, and also neoplasms of the thyroid. Two rats of each of the five groups were treated at 20 weeks of age with [14C]BOP. As measured by respiration of 14CO2, metabolism of BOP was faster in the two groups of male rats with the estradiol implant than in the other groups. DNA and RNA of the liver were isolated 6 h after treatment. The extent of methylation of liver DNA as 7-methylguanine and O6-methylguanine was higher in the females and in the feminized males than in the intact male rats, but when normalized to the dose of nitrosamine per unit body weight there was little difference among the five groups. JF - Chemico-biological interactions AU - Lijinsky, W AU - Thomas, B J AU - Kovatch, R M AD - NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, MD 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 111 EP - 119 VL - 66 IS - 1-2 SN - 0009-2797, 0009-2797 KW - Nitrosamines KW - 0 KW - Estradiol KW - 4TI98Z838E KW - nitrosobis(2-oxopropyl)amine KW - 60599-38-4 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Estradiol -- pharmacology KW - Liver Neoplasms -- chemically induced KW - Lung Neoplasms -- chemically induced KW - Male KW - Female KW - Alkylation KW - Nitrosamines -- pharmacology KW - Neoplasms, Experimental -- chemically induced KW - DNA -- metabolism KW - Feminization -- complications KW - Feminization -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78264909?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Effects+of+feminization+of+male+F344+rats+on+induction+of+tumors+and+on+nucleic+acid+alkylation+by+nitrosobis-%282-oxopropyl%29amine.&rft.au=Lijinsky%2C+W%3BThomas%2C+B+J%3BKovatch%2C+R+M&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=66&rft.issue=1-2&rft.spage=111&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-05 N1 - Date created - 1988-08-05 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: Chem Biol Interact 1988;67(1-2):169 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Carcinogenesis and nucleic acid alkylation by some oxygenated nitrosamines in rats and hamsters. AN - 78264567; 3383287 AB - A comparison has been made of the carcinogenic effects of nitroso-2,6-dimethylmorpholine and several hydroxylated acyclic nitrosodialkylamines derived from it or related to it in rats and Syrian hamsters. In rats nitrosodimethylmorpholine was the most potent, inducing mainly esophageal tumors. Nitrosodiethanolamine was the weakest of the five nitrosamines in both rats and hamsters. Tumors of the pancreas ducts were induced by four of the five compounds, but only in hamsters, and esophageal tumors appeared only in rats. Most of the nitrosamines induced tumors of liver and lung in both rats and hamsters. A study of alkylation of nucleic acids of the liver following treatment of rats and hamsters with the radiolabeled nitrosamines showed that nitrosodiethanolamine alkylated liver nucleic acids in rats to only a very small extent. The other four nitrosamines all gave rise to 7-methylation and O6-methylation of guanine residues in DNA of hamster liver and all but nitrosodimethylmorpholine in rat liver DNA, which corresponded quite well with the induction of liver tumors in the two species. Quantitatively, however, there was not a good correlation between liver DNA alkylation and the potency of the nitrosamine in inducing tumors. JF - Chemico-biological interactions AU - Lijinsky, W AU - Saavedra, J E AU - Kovatch, R M AD - NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, MD 21701. Y1 - 1988 PY - 1988 DA - 1988 SP - 37 EP - 47 VL - 66 IS - 1-2 SN - 0009-2797, 0009-2797 KW - Nitrosamines KW - 0 KW - N-nitroso-2,6-dimethylmorpholine KW - 1456-28-6 KW - N-nitrosodiethanolamine KW - 30YI1289VY KW - Diethylnitrosamine KW - 3IQ78TTX1A KW - diisopropanolnitrosamine KW - 4J072HB2ND KW - nitrosobis(2-oxopropyl)amine KW - 60599-38-4 KW - N-nitroso(2-hydroxypropyl)(2-oxopropyl)amine KW - 61499-28-3 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Rats KW - Diethylnitrosamine -- analogs & derivatives KW - Administration, Oral KW - Animals KW - Rats, Inbred F344 KW - Diethylnitrosamine -- administration & dosage KW - Liver Neoplasms -- chemically induced KW - Species Specificity KW - Methylation KW - Stomach KW - Cricetinae KW - Alkylation KW - Nitrosamines -- administration & dosage KW - Neoplasms, Experimental -- chemically induced KW - DNA -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78264567?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Carcinogenesis+and+nucleic+acid+alkylation+by+some+oxygenated+nitrosamines+in+rats+and+hamsters.&rft.au=Lijinsky%2C+W%3BSaavedra%2C+J+E%3BKovatch%2C+R+M&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=66&rft.issue=1-2&rft.spage=37&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-08-05 N1 - Date created - 1988-08-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Modulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in F344 rats by di(2-ethylhexyl)phthalate. AN - 78258578; 3378278 AB - The effects of cotreatment with a hyperlipidemic chemical, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and a hypolipidemic agent, di(2-ethylhexyl)-phthalate (DEHP), on lipid metabolism and toxicologic responses were studied in F344 rats. Treatment with TCDD alone (160 micrograms/kg) caused an increase in serum triglycerides and cholesterol while treatment with DEHP alone (2 g/kg/day) caused a decrease in triglycerides and cholesterol versus untreated controls. When administered before or after TCDD, DEHP caused a decrease in TCDD-induced hyperlipidemia. This change was attributed to enhanced hepatic peroxisomal beta-oxidation and decreased hepatic lipid synthesis resulting from treatment with DEHP. TCDD treatment produced a fatty liver, as determined by gravimetric analysis of extracted lipid and microscopic examination of liver sections which revealed extensive cytoplasmic vacuolization that stained positive with Oil Red 0, but did not induce peroxisomal beta-oxidation. Thus, an increase in hepatic or serum lipid levels is not sufficient for induction of peroxisome proliferation. Neither TCDD nor DEHP treatment affected mitochondrial beta-oxidation. Pretreatment of rats with DEHP, followed by daily exposure to this hypolipidemic agent after treatment with TCDD, had a partial protective effect against TCDD-induced fatty liver, body weight loss and mortality. Microscopic examination of liver sections confirmed the suppression of TCDD-induced fatty liver by pretreatment with DEHP. When DEHP treatment was initiated after the TCDD dose, there was less protection against the above parameters of TCDD toxicity. This study demonstrates that TCDD-induced fatty liver, hyperlipidemia and mortality can be antagonized by treatment with a hypolipidemic agent such as DEHP. JF - Chemico-biological interactions AU - Tomaszewski, K E AU - Montgomery, C A AU - Melnick, R L AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 205 EP - 222 VL - 65 IS - 3 SN - 0009-2797, 0009-2797 KW - Dioxins KW - 0 KW - Fatty Acids KW - Phthalic Acids KW - Polychlorinated Dibenzodioxins KW - Triglycerides KW - Cholesterol KW - 97C5T2UQ7J KW - Diethylhexyl Phthalate KW - C42K0PH13C KW - Oxidoreductases KW - EC 1.- KW - Acyl-CoA Oxidase KW - EC 1.3.3.6 KW - Index Medicus KW - Triglycerides -- blood KW - Microbodies -- enzymology KW - Animals KW - Liver -- pathology KW - Drug Interactions KW - Fatty Liver -- chemically induced KW - Vacuoles -- pathology KW - Liver -- metabolism KW - Fatty Acids -- metabolism KW - Rats KW - Oxidation-Reduction KW - Cholesterol -- blood KW - Rats, Inbred F344 KW - Oxidoreductases -- metabolism KW - Liver -- drug effects KW - Kinetics KW - Body Weight -- drug effects KW - Lethal Dose 50 KW - Fatty Liver -- pathology KW - Male KW - Lipid Metabolism KW - Phthalic Acids -- pharmacology KW - Polychlorinated Dibenzodioxins -- pharmacology KW - Diethylhexyl Phthalate -- toxicity KW - Polychlorinated Dibenzodioxins -- toxicity KW - Diethylhexyl Phthalate -- pharmacology KW - Dioxins -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78258578?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Canadian+Journal+of+Anesthesia&rft.atitle=Removing+nonessential+central+venous+catheters%3A+evaluation+of+a+quality+improvement+intervention&rft.au=Ilan%2C+Roy%2C+MD%3BDoan%2C+John%2C+MD%3BCload%2C+Bruce%2C+MD%2C+PhD%3BSquires%2C+Mae%2C+PhD%3BDay%2C+Andrew%2C+MSc&rft.aulast=Ilan&rft.aufirst=Roy&rft.date=2012-12-01&rft.volume=59&rft.issue=12&rft.spage=1102&rft.isbn=&rft.btitle=&rft.title=Canadian+Journal+of+Anesthesia&rft.issn=0832610X&rft_id=info:doi/10.1007%2Fs12630-012-9794-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-07-22 N1 - Date created - 1988-07-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Chemical carcinogenesis: from animal models to molecular models in one decade. AN - 78250109; 3287845 AB - During the last decade, progress in chemical carcinogenesis research has been substantial, and understanding the cellular changes and molecular causes of initiation, promotion, and malignant conversion appears to be within reach. Cancer begins as a carcinogen-induced genetic change in a single cell. The interaction of a particular carcinogen with specific genetic sites results, in part, from selectivity of metabolically activated carcinogens for particular nucleosides or gene sequences. In turn, modification of the molecular structure at specific genetic loci will have tissue-specific and species-specific consequences dependent on the expression of a particular gene, its sequence, and the function of the gene product in the target cell. It is likely that inactivation of regulatory regions, genomic rearrangements, and point mutations in coding sequences all can result in an altered cell phenotype. The rasH gene (and perhaps other members of the ras gene family) appears to be a common target for coding sequence mutations in the initiation of carcinogenesis in several organ sites and species by specific carcinogens. Whatever genetic mechanisms are involved, an initiated cell phenotype common to many epithelial cell types is observed. Initiated cells have an altered program of terminal differentiation, are resistant to cytotoxic substances or show altered requirements for specific growth factors or nutrients. These cells would have a selective growth advantage in cytostatic or cytotoxic situations or under conditions favoring terminal differentiation. Tumor promoters, some acting through specific cellular receptors, produce a tissue environment conductive to the selective clonal outgrowth of the initiated cell population resulting in a clinically evident premalignant lesion. The tissue specificity for most promoters depends on the ability of a particular agent to produce the selective conditions required for the initiated phenotype of that organ. At the molecular level, phorbol ester tumor promoters bind to and activate protein kinase C and transduce signals through this second-messenger pathway. Heterogeneity in the species of protein kinase C molecule expressed by normal and initiated epidermal cells could account for the differential response pattern observed in these cell types during skin tumor promotion. Malignant conversion of benign tumors requires further genetic changes in the tumor cell. Such changes could result from inherent instability in the genome of initiated cells, from spontaneous mutations more likely to occur in the expanding population of proliferating benign tumor cells, or by additional exposure to exogenous genotoxic agents.(ABSTRACT TRUNCATED AT 400 WORDS) JF - Advances in cancer research AU - Yuspa, S H AU - Poirier, M C AD - Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 25 EP - 70 VL - 50 SN - 0065-230X, 0065-230X KW - Carcinogens KW - 0 KW - Index Medicus KW - Rats KW - Animals KW - Oncogenes KW - DNA Repair KW - Carcinogens -- metabolism KW - Models, Molecular KW - Guinea Pigs KW - Humans KW - In Vitro Techniques KW - Dogs KW - Mice KW - Cricetinae KW - Neoplasms, Experimental -- chemically induced KW - Neoplasms, Experimental -- metabolism KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78250109?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+cancer+research&rft.atitle=Chemical+carcinogenesis%3A+from+animal+models+to+molecular+models+in+one+decade.&rft.au=Yuspa%2C+S+H%3BPoirier%2C+M+C&rft.aulast=Yuspa&rft.aufirst=S&rft.date=1988-01-01&rft.volume=50&rft.issue=&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Advances+in+cancer+research&rft.issn=0065230X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-07-05 N1 - Date created - 1988-07-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Primary cardiac hemangiosarcomas induced by 1,3-butadiene in B6C3F1 hybrid mice. AN - 78243253; 3375747 AB - Proliferative vascular lesions of the heart were found in mice exposed chronically to 1,3-butadiene by inhalation with an overall incidence of 30% in males and 43% in females. Based on histological criteria, the lesions were subclassified as endothelial hyperplasia with an incidence of 7% in males and 13% in females and hemangiosarcoma with an incidence of 23% and 30%, respectively. A dose-relationship for both lesions was observed in females, but not in males. The absence of a dose response in males was most likely due to the lower survival rate for high-dose animals (14%) when compared to the lower-dose animals (22%). Endothelial hyperplasia was characterized by widened vascular spaces lined by a single layer of plump endothelial cells. When cellular pleomorphism and piling up of endothelial nuclei were observed, the lesion was diagnosed as hemangiosarcoma. Ultrastructural examination of hemangiosarcomas revealed lumen formation, intercellular junctions and cytoplasmic filaments. Pinocytotic vesicles which are 1 of the characteristics of endothelial cells could not be identified with certainty. Weibel-Palade bodies were not detected in the neoplastic endothelium. Metastatic lesions were observed in liver, lung and kidney. To date, 1,3-butadiene is the only carcinogen reported that induces proliferative vascular lesions in the heart of mice. JF - Toxicologic pathology AU - Solleveld, H A AU - Miller, R A AU - Banas, D A AU - Boorman, G A AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 46 EP - 52 VL - 16 IS - 1 SN - 0192-6233, 0192-6233 KW - Butadienes KW - 0 KW - 1,3-butadiene KW - JSD5FGP5VD KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Hyperplasia KW - Sex Factors KW - Microscopy, Electron KW - Mice KW - Male KW - Female KW - Heart Neoplasms -- chemically induced KW - Butadienes -- toxicity KW - Heart Neoplasms -- pathology KW - Hemangiosarcoma -- ultrastructure KW - Hemangiosarcoma -- pathology KW - Hemangiosarcoma -- chemically induced KW - Heart Neoplasms -- ultrastructure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78243253?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+pathology&rft.atitle=Primary+cardiac+hemangiosarcomas+induced+by+1%2C3-butadiene+in+B6C3F1+hybrid+mice.&rft.au=Solleveld%2C+H+A%3BMiller%2C+R+A%3BBanas%2C+D+A%3BBoorman%2C+G+A&rft.aulast=Solleveld&rft.aufirst=H&rft.date=1988-01-01&rft.volume=16&rft.issue=1&rft.spage=46&rft.isbn=&rft.btitle=&rft.title=Toxicologic+pathology&rft.issn=01926233&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-07-14 N1 - Date created - 1988-07-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The role of the multichain IL-2 receptor complex in the control of normal and malignant T-cell proliferation. AN - 78239575; 2897696 AB - Antigen-induced activation of resting T cells induces the synthesis of interleukin-2 (IL-2), as well as the expression of specific cell surface receptors for this lymphokine. There are at least two forms of the cellular receptors for IL-2, one with a very high affinity and the other with a lower affinity. We have identified two IL-2 binding peptides, a 55-kd peptide reactive with the anti-Tac monoclonal antibody and a 75-kd non-Tac IL-2 binding peptide. Cell lines bearing either the p55, Tac, or the p75 peptide alone manifested low-affinity IL-2 binding, whereas cell lines bearing both peptides manifested both high- and low-affinity receptors. Fusion of cell membranes from low-affinity IL-2 binding cells bearing the Tac peptide alone with membranes from a cell line bearing the p75 peptide alone generated hybrid membranes bearing high-affinity receptors. We propose a multichain model for the high-affinity IL-2 receptor in which both the p55 Tac and the p75 IL-2 binding peptides are associated in a receptor complex. The p75 peptide is the receptor for IL-2 on large granular lymphocytes and is sufficient for the IL-2 activation of these cells. In contrast to resting T cells, human T-cell lymphotropic virus I-associated adult T-cell leukemia cells constitutively express large numbers of IL-2 receptors. Because IL-2 receptors are present on the malignant T cells but not on normal resting cells, clinical trials have been initiated in which patients with adult T-cell leukemia are being treated with either unmodified or toxin-conjugated forms of anti-Tac monoclonal antibody directed toward this growth factor receptor. JF - Progress in clinical and biological research AU - Waldmann, T A AU - Tsudo, M AD - Metabolism Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 283 EP - 293 VL - 262 SN - 0361-7742, 0361-7742 KW - Antibodies, Monoclonal KW - 0 KW - Antigens, CD27 KW - Antigens, Surface KW - Exotoxins KW - Immunotoxins KW - Receptors, Antigen, T-Cell KW - Receptors, Immunologic KW - Receptors, Interleukin-2 KW - Index Medicus KW - AIDS/HIV KW - Humans KW - Immunotoxins -- therapeutic use KW - Receptors, Antigen, T-Cell -- immunology KW - Exotoxins -- therapeutic use KW - Pseudomonas -- metabolism KW - Cloning, Molecular KW - Antibodies, Monoclonal -- therapeutic use KW - Antibodies, Monoclonal -- immunology KW - Receptors, Immunologic -- genetics KW - Deltaretrovirus Infections -- therapy KW - Deltaretrovirus Infections -- pathology KW - Deltaretrovirus Infections -- immunology KW - T-Lymphocytes -- metabolism KW - Receptors, Immunologic -- metabolism KW - Deltaretrovirus Infections -- metabolism KW - T-Lymphocytes -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78239575?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+clinical+and+biological+research&rft.atitle=The+role+of+the+multichain+IL-2+receptor+complex+in+the+control+of+normal+and+malignant+T-cell+proliferation.&rft.au=Waldmann%2C+T+A%3BTsudo%2C+M&rft.aulast=Waldmann&rft.aufirst=T&rft.date=1988-01-01&rft.volume=262&rft.issue=&rft.spage=283&rft.isbn=&rft.btitle=&rft.title=Progress+in+clinical+and+biological+research&rft.issn=03617742&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-30 N1 - Date created - 1988-06-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Molecular characterization and genetic mapping of class I and class II MHC genes of the domestic cat. AN - 78226681; 2897330 AB - The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from man and mouse. The cat possesses a minimum of 20 class I loci and 5 class II genes per haploid genome. Class I genes of the domestic cat expressed limited restriction fragment length polymorphism. The average percent difference of the size of DNA fragments between individual cats was 9.0%, a value five times lower than the value for mice, but comparable to the human DNA polymorphism level. Class I and class II genes were both genetically mapped to feline chromosome B2 using a panel of rodent x cat somatic cell hybrids. Since feline chromosome B2 is syntenically homologous to human chromosome 6 and mouse chromosome 17, these results affirm the linkage conservation of the MHC-containing linkage group in the three mammalian orders. JF - Immunogenetics AU - Yuhki, N AU - O'Brien, S J AD - Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21701-1013. Y1 - 1988 PY - 1988 DA - 1988 SP - 414 EP - 425 VL - 27 IS - 6 SN - 0093-7711, 0093-7711 KW - Index Medicus KW - Genetic Linkage KW - Mammals -- immunology KW - Animals KW - Polymorphism, Restriction Fragment Length KW - Hybrid Cells KW - Mammals -- genetics KW - Species Specificity KW - Chromosome Mapping KW - Cats -- genetics KW - Cats -- immunology KW - Major Histocompatibility Complex UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78226681?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Immunogenetics&rft.atitle=Molecular+characterization+and+genetic+mapping+of+class+I+and+class+II+MHC+genes+of+the+domestic+cat.&rft.au=Yuhki%2C+N%3BO%27Brien%2C+S+J&rft.aulast=Yuhki&rft.aufirst=N&rft.date=1988-01-01&rft.volume=27&rft.issue=6&rft.spage=414&rft.isbn=&rft.btitle=&rft.title=Immunogenetics&rft.issn=00937711&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-29 N1 - Date created - 1988-06-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Pulmonary elastic fiber degradation in paraquat toxicity. An electron microscopic immunohistochemical study. AN - 78225722; 3370614 AB - To study the morphologic alterations of pulmonary elastic fibers in cynomolgus monkeys with paraquat toxicity, peroxidase- and ferritin-labeled antielastin antibodies were used for the light and electron microscopic localization of elastin. One week after paraquat, alveolitis, tissue damage and alveolar dilatation were present; elastic fibers were frayed and more diffusely and intensely stained than those of control animals. In the latter, staining was localized in peripheral regions of the amorphous components and, to a lesser extent, in some microfibrils of elastic fibers. At 3 to 4 weeks, diffuse staining was evident in damaged interstitial elastic fibers and in newly formed elastic fibers in areas of intraalveolar fibrosis. At 8 weeks, the interstitium contained many elastic fibers which showed staining only in peripheral regions of the amorphous components. These observations suggest that: 1) preembedding immunohistochemical staining for elastin is localized in peripheral regions of normal elastic fibers because the antielastin antibody can penetrate into mature and undamaged amorphous components only to a very limited extent; 2) in early stages of paraquat toxicity this staining is more diffuse and intense because elastase from inflammatory cells partially degrades the elastic fibers and permits greater penetration of the antibody into the amorphous materials; 3) in later stages the staining pattern returns to normal as inflammation subsides and elastic fibers are repaired; however, newly formed elastic fibers in areas of intraalveolar fibrosis stain diffusely, reflecting increased penetration of the antibody because of immaturity and incomplete cross-linking, and 4) degeneration of elastic fibers of alveolar walls in paraquat lung may lead to alveolar dilatation, which is associated with irregular fibrosis and constitutes one of the processes of pulmonary structural remodeling in paraquat lung.(ABSTRACT TRUNCATED AT 250 WORDS) JF - Journal of submicroscopic cytology and pathology AU - Fukuda, Y AU - Ferrans, V J AD - Pathology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 15 EP - 23 VL - 20 IS - 1 SN - 1122-9497, 1122-9497 KW - Elastin KW - 9007-58-3 KW - Paraquat KW - PLG39H7695 KW - Index Medicus KW - Animals KW - Macaca fascicularis KW - Pulmonary Fibrosis -- pathology KW - Pulmonary Fibrosis -- chemically induced KW - Pulmonary Fibrosis -- physiopathology KW - Disease Models, Animal KW - Microscopy, Electron KW - Immunohistochemistry KW - Paraquat -- pharmacology KW - Paraquat -- administration & dosage KW - Elastin -- physiology KW - Lung -- drug effects KW - Lung -- pathology KW - Elastin -- immunology KW - Paraquat -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78225722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+submicroscopic+cytology+and+pathology&rft.atitle=Pulmonary+elastic+fiber+degradation+in+paraquat+toxicity.+An+electron+microscopic+immunohistochemical+study.&rft.au=Fukuda%2C+Y%3BFerrans%2C+V+J&rft.aulast=Fukuda&rft.aufirst=Y&rft.date=1988-01-01&rft.volume=20&rft.issue=1&rft.spage=15&rft.isbn=&rft.btitle=&rft.title=Journal+of+submicroscopic+cytology+and+pathology&rft.issn=11229497&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-24 N1 - Date created - 1988-06-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Commentary on the status of short-term tests for chemical carcinogens. AN - 78221339; 3286246 JF - Environmental and molecular mutagenesis AU - Shelby, M D AU - Zeiger, E AU - Tennant, R W AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 437 EP - 441 VL - 11 IS - 4 SN - 0893-6692, 0893-6692 KW - Carcinogens KW - 0 KW - Mutagens KW - Index Medicus KW - Rats KW - Animals KW - Predictive Value of Tests KW - Mice KW - Mutagens -- pharmacology KW - Carcinogens -- pharmacology KW - Mutagenicity Tests -- methods KW - Carcinogens -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78221339?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+and+molecular+mutagenesis&rft.atitle=Commentary+on+the+status+of+short-term+tests+for+chemical+carcinogens.&rft.au=Shelby%2C+M+D%3BZeiger%2C+E%3BTennant%2C+R+W&rft.aulast=Shelby&rft.aufirst=M&rft.date=1988-01-01&rft.volume=11&rft.issue=4&rft.spage=437&rft.isbn=&rft.btitle=&rft.title=Environmental+and+molecular+mutagenesis&rft.issn=08936692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-28 N1 - Date created - 1988-06-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Generation of superoxide anion and hydrogen peroxide during redox cycling of 5-(4-nitrophenyl)-penta-2,4-dienal by mammalian microsomes and enzymes. AN - 78206947; 2835186 AB - 5-(4-Nitrophenyl)penta-2,4-dienal (NPPD) stimulated NADPH-supported oxygen consumption by rat liver microsomes in a concentration-dependent manner. The NPPD stimulation of O2 uptake was not inhibited by metyrapone and was decreased in the presence of NADP+ and p-hydroxymercuribenzoate. These observations suggest that the NPPD initial reduction step is mediated by NADPH-cytochrome P-450 reductase and not by cytochrome P-450. Spin-trapping studies using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) revealed the formation of superoxide anion upon incubation of NPPD, NADPH, DMPO and rat liver microsomes. Hydrogen peroxide generation was also detected in these incubations, thus confirming redox cycling of NPPD under aerobic conditions. NPPD stimulated oxygen consumption, superoxide anion formation and hydrogen peroxide generation by rat kidney, testes and brain microsomes. Other enzymes capable of nitroreduction (NADH dehydrogenase, xanthine oxidase, glutathione reductase, and NADP+ ferredoxin oxidoreductase) were also found to stimulate redox cycling of NPPD. The ability of NPPD to induce superoxide anion and hydrogen peroxide formation might play a role in its reported mutagenicity. JF - Chemico-biological interactions AU - Docampo, R AU - Moreno, S N AU - Mason, R P AD - Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, N.C. 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 123 EP - 131 VL - 65 IS - 2 SN - 0009-2797, 0009-2797 KW - Nitrobenzenes KW - 0 KW - Superoxides KW - 11062-77-4 KW - 5-(4-nitrophenyl)-2,4-pentadienal KW - 2608-48-2 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Index Medicus KW - Rats KW - Oxidation-Reduction KW - Animals KW - Oxygen Consumption -- drug effects KW - Kidney -- metabolism KW - Testis -- metabolism KW - Biotransformation KW - Microsomes, Liver -- metabolism KW - Kinetics KW - In Vitro Techniques KW - Brain -- metabolism KW - Lung -- metabolism KW - Male KW - Superoxides -- metabolism KW - Microsomes -- metabolism KW - Hydrogen Peroxide -- metabolism KW - Nitrobenzenes -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78206947?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Generation+of+superoxide+anion+and+hydrogen+peroxide+during+redox+cycling+of+5-%284-nitrophenyl%29-penta-2%2C4-dienal+by+mammalian+microsomes+and+enzymes.&rft.au=Docampo%2C+R%3BMoreno%2C+S+N%3BMason%2C+R+P&rft.aulast=Docampo&rft.aufirst=R&rft.date=1988-01-01&rft.volume=65&rft.issue=2&rft.spage=123&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-14 N1 - Date created - 1988-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Dopamine in the extrapyramidal motor function. A study based upon the MPTP-induced primate model of parkinsonism. AN - 78201856; 3259118 JF - Annals of the New York Academy of Sciences AU - Chiueh, C C AD - Clinical Brain Imaging Section, National Institute of Mental Health, Bethesda, Maryland 20892-1000. Y1 - 1988 PY - 1988 DA - 1988 SP - 226 EP - 238 VL - 515 SN - 0077-8923, 0077-8923 KW - Pyridines KW - 0 KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - 9P21XSP91P KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Animals KW - Macaca KW - Brain -- metabolism KW - Mice KW - Autoradiography KW - Brain -- diagnostic imaging KW - Substantia Nigra -- physiopathology KW - Corpus Striatum -- physiopathology KW - Substantia Nigra -- pathology KW - Neurons -- physiology KW - Mice, Inbred C57BL KW - Tomography, Emission-Computed KW - Corpus Striatum -- pathology KW - Female KW - Male KW - Parkinson Disease, Secondary -- physiopathology KW - Parkinson Disease, Secondary -- chemically induced KW - Parkinson Disease, Secondary -- pathology KW - Extrapyramidal Tracts -- physiology KW - Dopamine -- physiology KW - Dopamine -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78201856?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Dopamine+in+the+extrapyramidal+motor+function.+A+study+based+upon+the+MPTP-induced+primate+model+of+parkinsonism.&rft.au=Chiueh%2C+C+C&rft.aulast=Chiueh&rft.aufirst=C&rft.date=1988-01-01&rft.volume=515&rft.issue=&rft.spage=226&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Antitumor effect and cardiotoxicity of a doxorubicin-lecithin association. AN - 78186840; 3358634 AB - A doxorubicin-lecithin preparation was tested in vitro on B16 melanoma and Lewis lung carcinoma cells, and in vivo on C57BL/6 mice inoculated with 3LL cells. Results obtained demonstrated that the preparation possesses the same antitumor activity as doxorubicin. The cardiotoxicity of doxorubicin and of the doxorubicin-lecithin association were studied for 120 days after the end of the treatments in Wistar rats inoculated once a week for 5 weeks with equivalent doses of the drugs. Myocardial lesions were observed in both the groups of animals, but their severity and extent were reduced in rats treated with the doxorubicin-lecithin association, and their onset was also delayed. JF - Anticancer research AU - Bellelli, A AU - Giomini, M AU - Giuliani, A M AU - Giustini, M AU - Lorenzon, I AU - Rusconi, V AU - Sezzi, M L AU - Trotta, E AU - Belleli, L AD - Regina Elena National Cancer Institute, Roma, Italy. PY - 1988 SP - 177 EP - 186 VL - 8 IS - 1 SN - 0250-7005, 0250-7005 KW - Phosphatidylcholines KW - 0 KW - Doxorubicin KW - 80168379AG KW - Index Medicus KW - Rats KW - Animals KW - Tumor Cells, Cultured -- drug effects KW - Electrocardiography KW - Myocardium -- ultrastructure KW - Mice KW - Cardiomyopathies -- pathology KW - Carcinoma -- drug therapy KW - Melanoma, Experimental -- drug therapy KW - Doxorubicin -- administration & dosage KW - Cardiomyopathies -- chemically induced KW - Phosphatidylcholines -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78186840?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+research&rft.atitle=Antitumor+effect+and+cardiotoxicity+of+a+doxorubicin-lecithin+association.&rft.au=Bellelli%2C+A%3BGiomini%2C+M%3BGiuliani%2C+A+M%3BGiustini%2C+M%3BLorenzon%2C+I%3BRusconi%2C+V%3BSezzi%2C+M+L%3BTrotta%2C+E%3BBelleli%2C+L&rft.aulast=Bellelli&rft.aufirst=A&rft.date=1988-01-01&rft.volume=8&rft.issue=1&rft.spage=177&rft.isbn=&rft.btitle=&rft.title=Anticancer+research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-16 N1 - Date created - 1988-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Substrates for protein kinase C in a cell free preparation of rat aorta smooth muscles. AN - 78158552; 2832673 AB - Protein phosphorylation has been studied in a cell free system of rat aorta smooth muscles. Addition of Ca2+ caused phosphorylation of several proteins. The addition of phosphatidylserine or calmodulin together with Ca2+ further increased the phosphorylation of proteins with apparent molecular weights of 20 and 92.5 kilodaltons. The activators of protein kinase C, 12-0-tetradecanoylphorbol-13-acetate and 1,2-diolein, increased phosphorylation of the protein bands of similar molecular weight to those increased by phosphatidylserine in the presence of Ca2+, whereas the biologically inactive phorbol ester, 4 alpha-phorbol-12,13 didecanoate (4 alpha PDD) failed to change the pattern of protein phosphorylation. These results show that proteins present in smooth muscle of rat aorta with molecular weights of 20 and 92.5 kilodaltons are substrates for protein kinase C. JF - Life sciences AU - Nakaki, T AU - Wise, B C AU - Chuang, D M AD - Laboratory of Preclinical Pharmacology, National Institute of Mental Health, St. Elizabeths Hospital, Washington, D.C. 20032. Y1 - 1988 PY - 1988 DA - 1988 SP - 1315 EP - 1321 VL - 42 IS - 13 SN - 0024-3205, 0024-3205 KW - Calmodulin KW - 0 KW - Diglycerides KW - Phosphatidylserines KW - Phosphoproteins KW - Cyclic AMP KW - E0399OZS9N KW - Protein Kinase C KW - EC 2.7.11.13 KW - Cyclic GMP KW - H2D2X058MU KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Calcium KW - SY7Q814VUP KW - diolein KW - Z3MP1W91CW KW - Index Medicus KW - Animals KW - Cyclic GMP -- pharmacology KW - Calcium -- pharmacology KW - Molecular Weight KW - Rats, Inbred Strains KW - Rats KW - Diglycerides -- pharmacology KW - Phosphorylation KW - Phosphatidylserines -- pharmacology KW - Cyclic AMP -- pharmacology KW - Aorta -- enzymology KW - Enzyme Activation -- drug effects KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Calmodulin -- pharmacology KW - Male KW - Phosphoproteins -- metabolism KW - Cell-Free System KW - Protein Kinase C -- metabolism KW - Muscle, Smooth -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78158552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Substrates+for+protein+kinase+C+in+a+cell+free+preparation+of+rat+aorta+smooth+muscles.&rft.au=Nakaki%2C+T%3BWise%2C+B+C%3BChuang%2C+D+M&rft.aulast=Nakaki&rft.aufirst=T&rft.date=1988-01-01&rft.volume=42&rft.issue=13&rft.spage=1315&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-22 N1 - Date created - 1988-04-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Urinary porphyrins in children exposed transplacentally to polyhalogenated aromatics in Taiwan. AN - 78157203; 3128188 AB - In 1979, there was a large (greater than 2,000 cases) outbreak of poisoning due to contaminated rice oil in central Taiwan. The causal agent was a mixture of thermally degraded polychlorinated biphenyls (PCBs), polychlorinated quaterphenyls, and polychlorinated dibenzofurans, which had become mixed with the oil during processing. Patients remained symptomatic for several years afterward, and the chemicals persisted in their tissue. Women who became pregnant had children with high perinatal mortality and a dysmorphic syndrome. We examined urines from 75 children born to exposed mothers after the oil was confiscated, 74 controls, and 12 sibs of the exposed children. Four of the transplacentally exposed children, 2 controls, and 1 sib had a type B hepatic porphyria (i.e., uroporphyrin greater than coproporphyrin); total porphyrin excretion was elevated in the exposed children as a group (95 vs. 81 micrograms/L); and 8 of the 75 exposed children and 2 controls had total urinary porphyrin concentrations of greater than 200 micrograms/L. JF - Archives of environmental health AU - Gladen, B C AU - Rogan, W J AU - Ragan, N B AU - Spierto, F W AD - Statistics and Biomathematics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. PY - 1988 SP - 54 EP - 58 VL - 43 IS - 1 SN - 0003-9896, 0003-9896 KW - Porphyrins KW - 0 KW - Creatinine KW - AYI8EX34EU KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Abridged Index Medicus KW - Index Medicus KW - Skin Diseases -- urine KW - Taiwan KW - Albuminuria -- urine KW - Creatinine -- urine KW - Humans KW - Chemical and Drug Induced Liver Injury KW - Child KW - Skin Diseases -- chemically induced KW - Male KW - Female KW - Pregnancy KW - Child, Preschool KW - Porphyrins -- urine KW - Porphyrias -- chemically induced KW - Polychlorinated Biphenyls -- poisoning KW - Liver Diseases -- urine KW - Porphyrias -- urine KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78157203?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+environmental+health&rft.atitle=Urinary+porphyrins+in+children+exposed+transplacentally+to+polyhalogenated+aromatics+in+Taiwan.&rft.au=Gladen%2C+B+C%3BRogan%2C+W+J%3BRagan%2C+N+B%3BSpierto%2C+F+W&rft.aulast=Gladen&rft.aufirst=B&rft.date=1988-01-01&rft.volume=43&rft.issue=1&rft.spage=54&rft.isbn=&rft.btitle=&rft.title=Archives+of+environmental+health&rft.issn=00039896&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-03 N1 - Date created - 1988-05-03 N1 - Date revised - 2017-01-14 N1 - SuppNotes - Erratum In: Arch Environ Health 1988 Sep-Oct;43(5):348 N1 - Last updated - 2017-01-19 ER - TY - JOUR T1 - Treatment of high-risk sarcomas in children and young adults: analysis of local control using intensive combined modality therapy. AN - 78156829; 3352773 AB - Although combination chemotherapy and local irradiation are quite effective treatment for some children and young adults with small round cell sarcomas, high-risk patient groups, including patients with localized disease of the trunk and proximal extremity and those who present with metastases, continue to fare poorly with standard combined modality therapy. In an attempt to improve the local and systemic response of these tumors, an intensive treatment protocol was designed that integrates five cycles of chemotherapy with vincristine, doxorubicin, and cyclophosphamide (VADRIAC) plus radiation therapy to the primary tumor (55-60 Gy), bone, and soft tissue metastases (45-50 Gy). Patients achieving complete response to this induction therapy receive intensification treatment with total body irradiation (8.0 Gy), a cycle of VADRIAC, and autologous bone marrow transplantation. All treatment is completed within 6-7 months. From January 1983 to February 1986, 76 consecutive, previously untreated patients were entered in this study; 75 patients are evaluable. Twenty-five patients were diagnosed with rhabdomyosarcoma, 23 with Ewing's sarcoma, 15 with primitive neuroepithelioma, 12 with primitive sarcoma, and 1 patient with metastatic neuroblastoma. Forty-three patients (57%) had metastases at presentation. Overall, 68 of 75 patients (91%) achieved complete response. Fifty-eight of 61 patients with measurable soft tissue masses at the primary site had greater than or equal to 50% tumor reduction with two cycles of chemotherapy prior to local irradiation. Seven patients failed to have complete response at the primary site following five cycles of chemotherapy and local external beam irradiation, although 3 were subsequently rendered locally disease free by intraoperative radiotherapy (2 patients) or surgery (1 patient).(ABSTRACT TRUNCATED AT 250 WORDS) JF - NCI monographs : a publication of the National Cancer Institute AU - Kinsella, T J AU - Miser, J S AU - Triche, T J AU - Horvath, K AU - Glatstein, E AD - Radiation Oncology Branch, National Cancer Institute, Bethesda, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 291 EP - 296 IS - 6 SN - 0893-2751, 0893-2751 KW - Vincristine KW - 5J49Q6B70F KW - Doxorubicin KW - 80168379AG KW - Cyclophosphamide KW - 8N3DW7272P KW - Index Medicus KW - Doxorubicin -- adverse effects KW - Vincristine -- adverse effects KW - Combined Modality Therapy KW - Risk Factors KW - Humans KW - Adult KW - Child KW - Adolescent KW - Male KW - Female KW - Radiotherapy -- adverse effects KW - Cyclophosphamide -- adverse effects KW - Child, Preschool KW - Antineoplastic Combined Chemotherapy Protocols KW - Sarcoma -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78156829?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.atitle=Treatment+of+high-risk+sarcomas+in+children+and+young+adults%3A+analysis+of+local+control+using+intensive+combined+modality+therapy.&rft.au=Kinsella%2C+T+J%3BMiser%2C+J+S%3BTriche%2C+T+J%3BHorvath%2C+K%3BGlatstein%2C+E&rft.aulast=Kinsella&rft.aufirst=T&rft.date=1988-01-01&rft.volume=&rft.issue=6&rft.spage=291&rft.isbn=&rft.btitle=&rft.title=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.issn=08932751&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-03 N1 - Date created - 1988-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Malignant melanoma in the printing industry. AN - 78153760; 3354581 AB - To evaluate a recent report of malignant melanoma among printers, we used the Cancer-Environment Registry of Sweden, which links national cancer incidence and employment data. Employees in the newspaper printing industry had almost twice the incidence of melanoma than expected (p less than 0.01). Elevated risks were found not only among typographers and machine repairers, but also among journalists, editors, and business executives in the printing industry. Further studies are needed to clarify this relationship and identify specific exposures that may be responsible. JF - American journal of industrial medicine AU - McLaughlin, J K AU - Malker, H S AU - Blot, W J AU - Ericsson, J L AU - Gemne, G AU - Fraumeni, J F AD - Epidemiology and Biostatistics Program, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 301 EP - 304 VL - 13 IS - 2 SN - 0271-3586, 0271-3586 KW - Index Medicus KW - Registries KW - Risk Factors KW - Humans KW - Sweden KW - Printing KW - Skin Neoplasms -- epidemiology KW - Occupational Diseases -- epidemiology KW - Melanoma -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78153760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Malignant+melanoma+in+the+printing+industry.&rft.au=McLaughlin%2C+J+K%3BMalker%2C+H+S%3BBlot%2C+W+J%3BEricsson%2C+J+L%3BGemne%2C+G%3BFraumeni%2C+J+F&rft.aulast=McLaughlin&rft.aufirst=J&rft.date=1988-01-01&rft.volume=13&rft.issue=2&rft.spage=301&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-22 N1 - Date created - 1988-04-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - ADP-ribosylation of guanyl nucleotide-binding regulatory proteins by bacterial toxins. AN - 78152266; 3128060 JF - Advances in enzymology and related areas of molecular biology AU - Moss, J AU - Vaughan, M AD - Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 303 EP - 379 VL - 61 SN - 0065-258X, 0065-258X KW - Bacterial Toxins KW - 0 KW - Enterotoxins KW - Escherichia coli Proteins KW - Virulence Factors, Bordetella KW - heat-labile enterotoxin, E coli KW - Adenosine Diphosphate Ribose KW - 20762-30-5 KW - Cholera Toxin KW - 9012-63-9 KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - Pentosyltransferases KW - Pertussis Toxin KW - EC 2.4.2.31 KW - GTP Phosphohydrolases KW - EC 3.6.1.- KW - GTP-Binding Proteins KW - Adenylyl Cyclases KW - EC 4.6.1.1 KW - Index Medicus KW - Neutrophils -- drug effects KW - Animals KW - Adenylyl Cyclases -- analysis KW - Humans KW - GTP Phosphohydrolases -- analysis KW - Enterotoxins -- pharmacology KW - Bacterial Toxins -- pharmacology KW - Pentosyltransferases -- analysis KW - Virulence Factors, Bordetella -- pharmacology KW - GTP-Binding Proteins -- metabolism KW - Cholera Toxin -- pharmacology KW - Adenosine Diphosphate Ribose -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78152266?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+enzymology+and+related+areas+of+molecular+biology&rft.atitle=ADP-ribosylation+of+guanyl+nucleotide-binding+regulatory+proteins+by+bacterial+toxins.&rft.au=Moss%2C+J%3BVaughan%2C+M&rft.aulast=Moss&rft.aufirst=J&rft.date=1988-01-01&rft.volume=61&rft.issue=&rft.spage=303&rft.isbn=&rft.btitle=&rft.title=Advances+in+enzymology+and+related+areas+of+molecular+biology&rft.issn=0065258X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-29 N1 - Date created - 1988-04-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Comparative toxicity and renal distribution of the platinum analogs tetraplatin, CHIP, and cisplatin at equimolar doses in the Fischer 344 rat. AN - 78151602; 3350229 AB - Tetraplatin [tetrachloro(dl-trans)1,2-diaminocyclohexane platinum(IV), NSC-363812] is a new anticancer platinum drug analog targeted for clinical development because of its effectiveness against cisplatin-resistant tumor cell lines and its improved formulation. The toxicity of tetraplatin was compared at equimolar doses to that of cisplatin [cis-diamminedichlorophatinum(II)] and CHIP [cis-dichloro,trans-dihydroxybis-isopropylamine platinum(IV), NSC-256927]. Adult male Fischer 344 rats received an iv bolus injection of 6.7, 13.3, 26.7, or 53.3 mumol/kg of one of these drugs in saline and were killed on Day 1, 3, 5, 8, or 15 postinjection for assessment of toxicity with emphasis on evaluation of nephrotoxicity. Rats to be killed on Day 15 were housed in metabolism cages for daily urine collection. Tetraplatin was less nephrotoxic than cisplatin at equimolar doses; CHIP was not nephrotoxic at these doses. Renal platinum contents were similar after all three drugs and did not appear to be related directly to the nephrotoxicity. Nephrotoxicity was detected 4-5 days after 6.7 mumol/kg cisplatin, was localized to the corticomedullary junction, and progressed with time and dose. Tetraplatin-induced alterations of renal function were first observed after 13.4 mumol/kg on Day 4 as an elevation of urine volume (up to 10-fold) and a smaller elevation of urinary glucose excretion. Tetraplatin lesions were localized in the mid- and outer cortex and, even at the highest dose, were less severe than those observed with cisplatin. There were other prominent toxic effects of tetraplatin, such as gastrointestinal toxicity and myelosuppression, which indicate that factors other than comparative nephrotoxicity may impact the clinical potential of this new agent. JF - Fundamental and applied toxicology : official journal of the Society of Toxicology AU - Smith, J H AU - Smith, M A AU - Litterst, C L AU - Copley, M P AU - Uozumi, J AU - Boyd, M R AD - Laboratory of Experimental Therapeutics and Metabolism, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 45 EP - 61 VL - 10 IS - 1 SN - 0272-0590, 0272-0590 KW - Antineoplastic Agents KW - 0 KW - Organoplatinum Compounds KW - iproplatin KW - 5R9F9NE9Z2 KW - Cisplatin KW - Q20Q21Q62J KW - ormaplatin KW - SFK1SGY8V1 KW - Index Medicus KW - Rats KW - Kidney Diseases -- pathology KW - Animals KW - Rats, Inbred F344 KW - Digestive System -- drug effects KW - Body Weight -- drug effects KW - Male KW - Kidney Diseases -- chemically induced KW - Cisplatin -- pharmacokinetics KW - Organoplatinum Compounds -- pharmacokinetics KW - Kidney -- metabolism KW - Kidney -- pathology KW - Cisplatin -- toxicity KW - Antineoplastic Agents -- pharmacokinetics KW - Antineoplastic Agents -- toxicity KW - Organoplatinum Compounds -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78151602?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.atitle=Comparative+toxicity+and+renal+distribution+of+the+platinum+analogs+tetraplatin%2C+CHIP%2C+and+cisplatin+at+equimolar+doses+in+the+Fischer+344+rat.&rft.au=Smith%2C+J+H%3BSmith%2C+M+A%3BLitterst%2C+C+L%3BCopley%2C+M+P%3BUozumi%2C+J%3BBoyd%2C+M+R&rft.aulast=Smith&rft.aufirst=J&rft.date=1988-01-01&rft.volume=10&rft.issue=1&rft.spage=45&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-26 N1 - Date created - 1988-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Lack of cage effects on liver tumor incidence in B6C3F1 mice. AN - 78145744; 3350226 AB - Twenty-three chemicals evaluated as hepatocarcinogens in B6C3F1 mice by the National Toxicology Program (NTP) and nine showing equivocal liver tumor effects were examined to determine if the occurrence of liver neoplasms was associated with caging, i.e., if there were significant differences in tumor incidence among cages. A total of 79 dosed groups showing increased liver tumor incidence were evaluated. The analysis showed that the number of instances in which liver tumors showed significant cage effects agreed closely with chance expectation. Experimental design protocols now utilized by the NTP (including random assignment of columns of cages to dosed and control groups, periodic rotation of cage location, and individual caging of mice) further reduce the likelihood that factors associated with the housing of the animals could influence tumor incidence. JF - Fundamental and applied toxicology : official journal of the Society of Toxicology AU - Haseman, J K AD - Division of Biometry and Risk Assessment, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 179 EP - 187 VL - 10 IS - 1 SN - 0272-0590, 0272-0590 KW - Carcinogens KW - 0 KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Mice KW - Male KW - Female KW - Liver Neoplasms, Experimental -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78145744?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.atitle=Lack+of+cage+effects+on+liver+tumor+incidence+in+B6C3F1+mice.&rft.au=Haseman%2C+J+K&rft.aulast=Haseman&rft.aufirst=J&rft.date=1988-01-01&rft.volume=10&rft.issue=1&rft.spage=179&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-26 N1 - Date created - 1988-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Phase III trial of irradiation plus chemotherapy for patients with hepatic metastases and hepatoma: experience of the Northern California Oncology Group. AN - 78144980; 2832763 AB - The effects of iv or intra-arterial chemotherapy added to hepatic irradiation were evaluated in a 3-arm randomized trial. Patients with predominantly hepatic metastases or with hepatoma were eligible. They were randomized to receive 2,100 cGy in seven fractions alone or with 5-fluorouracil given either intra-arterially or by iv infusion; doxorubicin and mitomycin were given by bolus simultaneously with the radiation in a single course. A total of 166 patients were entered in the study. Toxicity was acceptable, with no sign of enhanced radiation damage. Response was evaluated 4-6 weeks after treatment. No complete responses were seen, but partial responses greater than or equal to 50% were observed in the groups treated with radiation only (17%), radiation plus drug given iv (25%), and radiation plus drug given intra-arterially (20%) (P greater than .3). Disease progression occurred in a larger number of patients who received radiation only (29%) at 6 weeks than in the other 2 groups (7% and 18%, respectively; P less than .03). Thus, in terms of local response duration, the addition of chemotherapy enhanced the effect of the radiation. Survival was not different among the 3 groups. JF - NCI monographs : a publication of the National Cancer Institute AU - Friedman, M A AU - Phillips, T L AU - Hannigan, J F AU - Carter, S K AD - Division of Cancer Treatment, National Cancer Institute, Bethesda, MD. Y1 - 1988 PY - 1988 DA - 1988 SP - 259 EP - 264 IS - 6 SN - 0893-2751, 0893-2751 KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Fluorouracil -- administration & dosage KW - Combined Modality Therapy KW - Radiotherapy Dosage KW - Humans KW - Adult KW - Clinical Trials as Topic KW - Aged KW - Middle Aged KW - Male KW - Female KW - Liver Neoplasms -- therapy KW - Carcinoma, Hepatocellular -- secondary KW - Liver Neoplasms -- mortality KW - Carcinoma, Hepatocellular -- therapy KW - Liver Neoplasms -- secondary KW - Carcinoma, Hepatocellular -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78144980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.atitle=Phase+III+trial+of+irradiation+plus+chemotherapy+for+patients+with+hepatic+metastases+and+hepatoma%3A+experience+of+the+Northern+California+Oncology+Group.&rft.au=Friedman%2C+M+A%3BPhillips%2C+T+L%3BHannigan%2C+J+F%3BCarter%2C+S+K&rft.aulast=Friedman&rft.aufirst=M&rft.date=1988-01-01&rft.volume=&rft.issue=6&rft.spage=259&rft.isbn=&rft.btitle=&rft.title=NCI+monographs+%3A+a+publication+of+the+National+Cancer+Institute&rft.issn=08932751&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-03 N1 - Date created - 1988-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Behavioral interactions between ethanol and imidazodiazepines with high affinities for benzodiazepine receptors. AN - 78144479; 2832675 AB - The intrinsic effects of two imidazodiazepines RO 15-3505 and RO 17-1812 on the behavior of mice in a holeboard test were investigated. The interactions of these two drugs with ethanol were also studied. RO 15-3505 (0.75-6.0 mg/kg) failed to significantly alter either exploratory head-dipping or locomotor activity when administered alone but doses of 0.75 and 1.5 mg/kg reversed the reduction in the number of head-dips caused by ethanol (2 g/kg) and partially reversed ethanol's locomotor stimulant action. In contrast, RO 17-1812 (0.75-6.0 mg/kg) increased locomotor activity when administered alone, and enhanced the reduction in exploration caused by ethanol. Neither RO 15-3505 nor RO 17-1812 altered blood alcohol concentrations suggesting a pharmacodynamic basis for these interactions. The results suggest that in the holeboard test the interactions of imidazodiazepines with ethanol are related to the nature of their interaction with benzodiazepine receptors, inverse agonists antagonising and agonists enhancing ethanol's effects on exploration. JF - Life sciences AU - Lister, R G AD - Laboratory of Clinical Studies, NIAAA, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 1385 EP - 1393 VL - 42 IS - 14 SN - 0024-3205, 0024-3205 KW - Benzodiazepinones KW - 0 KW - Receptors, GABA-A KW - Benzodiazepines KW - 12794-10-4 KW - Ethanol KW - 3K9958V90M KW - Ro 15-3505 KW - 78756-33-9 KW - Ro 17-1812 KW - 90450-01-4 KW - Index Medicus KW - Animals KW - Drug Interactions KW - Receptors, GABA-A -- physiology KW - Benzodiazepines -- antagonists & inhibitors KW - Receptors, GABA-A -- drug effects KW - Motor Activity -- drug effects KW - Mice KW - Male KW - Ethanol -- blood KW - Behavior, Animal -- drug effects KW - Ethanol -- pharmacology KW - Benzodiazepinones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78144479?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Behavioral+interactions+between+ethanol+and+imidazodiazepines+with+high+affinities+for+benzodiazepine+receptors.&rft.au=Lister%2C+R+G&rft.aulast=Lister&rft.aufirst=R&rft.date=1988-01-01&rft.volume=42&rft.issue=14&rft.spage=1385&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-25 N1 - Date created - 1988-04-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - In vivo biochemical indices of nephrotoxicity of platinum analogs tetraplatin, CHIP, and cisplatin in the Fischer 344 rat. AN - 78138984; 3350230 AB - In vivo biochemical indices of nephrotoxicity were investigated in Fischer 344 rats treated with a new platinum analog, tetraplatin [tetrachloro(dl-trans)1,2-diaminocyclohexane platinum(IV), NSC-363812], in comparison with rats receiving equimolar dosages of cisplatin and CHIP [cis-dichloro,trans-dihydroxybis-isopropylamine platinum(IV), NSC-256927]. The goals of this study were to assess the comparative nephrotoxicities and to determine which battery of tests might be useful for the assessment of platinum analog-induced nephrotoxicity in future clinical investigations of these drugs. An iv bolus injection of 6.7, 13.3, 26.7, and 53.3 mumol/kg of each drug in saline was administered and assessment of biochemical parameters was conducted for 15 days postinjection. A combination of urinary enzyme and protein excretion rates along with blood urea nitrogen (BUN) determinations was used to assess the nephrotoxicity of these compounds. At equimolar dosages, tetraplatin appeared to be less nephrotoxic than cisplatin, and CHIP was not nephrotoxic. At all dosages tested, cisplatin increased the rate of urinary excretion of protein, lactate dehydrogenase (LDH), and N-acetylglucosaminidase (NAG) between Days 1 and 5. Tetraplatin did not affect these parameters until the 13.3 mumol/kg dosage. Cisplatin had little effect on the excretion rates of the brush border enzymes alkaline phosphatase and maltase, whereas tetraplatin caused an initial elevation with delayed onset of peak excretion rates at 8 days postinjection. Changes in BUN were not evident until after the 13.3 mumol/kg dosage of cisplatin and the 26.7 mumol/kg dosage of tetraplatin. BUN was useful for ranking the relative toxicities of the three compounds tested, but was not as sensitive in detecting the onset of injury that correlated with early histopathological changes. Tetraplatin appeared to be less nephrotoxic than cisplatin on an equimolar basis and the specific manifestations of its toxicity were different from those observed with cisplatin. Urinary excretion rates for LDH, NAG, and protein proved to be sensitive indicators of platinum analog-induced nephrotoxicity. These indices, combined with BUN determinations and functional assessments, facilitated comparisons of the nephrotoxicity induced by cisplatin and tetraplatin in rats. JF - Fundamental and applied toxicology : official journal of the Society of Toxicology AU - Smith, M A AU - Smith, J H AU - Litterst, C L AU - Copley, M P AU - Uozumi, J AU - Boyd, M R AD - Laboratory of Experimental Therapeutics and Metabolism, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 62 EP - 72 VL - 10 IS - 1 SN - 0272-0590, 0272-0590 KW - Antineoplastic Agents KW - 0 KW - Electrolytes KW - Organoplatinum Compounds KW - iproplatin KW - 5R9F9NE9Z2 KW - Cisplatin KW - Q20Q21Q62J KW - ormaplatin KW - SFK1SGY8V1 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Proteinuria -- urine KW - Blood Urea Nitrogen KW - Electrolytes -- urine KW - Male KW - Proteinuria -- chemically induced KW - Kidney Diseases -- pathology KW - Cisplatin -- toxicity KW - Antineoplastic Agents -- toxicity KW - Organoplatinum Compounds -- toxicity KW - Kidney Diseases -- enzymology KW - Kidney Diseases -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78138984?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.atitle=In+vivo+biochemical+indices+of+nephrotoxicity+of+platinum+analogs+tetraplatin%2C+CHIP%2C+and+cisplatin+in+the+Fischer+344+rat.&rft.au=Smith%2C+M+A%3BSmith%2C+J+H%3BLitterst%2C+C+L%3BCopley%2C+M+P%3BUozumi%2C+J%3BBoyd%2C+M+R&rft.aulast=Smith&rft.aufirst=M&rft.date=1988-01-01&rft.volume=10&rft.issue=1&rft.spage=62&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-26 N1 - Date created - 1988-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Control of parathyroid (PTH) secretion is mediated through calcium channels. AN - 78135963; 2450376 JF - Progress in clinical and biological research AU - Fitzpatrick, L A AU - Aurbach, G D AD - Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 47 EP - 52 VL - 252 SN - 0361-7742, 0361-7742 KW - Calcium Channel Agonists KW - 0 KW - Calcium Channel Blockers KW - Ion Channels KW - Marine Toxins KW - Nicotinic Acids KW - Oxadiazoles KW - Oxocins KW - Parathyroid Hormone KW - PN 202-791 KW - 101342-80-7 KW - maitotoxin KW - 9P59GES78D KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Marine Toxins -- pharmacology KW - Animals KW - Nicotinic Acids -- pharmacology KW - Cattle KW - Calcium Channel Blockers -- pharmacology KW - Parathyroid Glands -- metabolism KW - In Vitro Techniques KW - Parathyroid Glands -- drug effects KW - Parathyroid Glands -- secretion KW - Calcium Channel Agonists -- pharmacology KW - Calcium -- metabolism KW - Parathyroid Hormone -- secretion KW - Ion Channels -- drug effects KW - Ion Channels -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78135963?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+clinical+and+biological+research&rft.atitle=Control+of+parathyroid+%28PTH%29+secretion+is+mediated+through+calcium+channels.&rft.au=Fitzpatrick%2C+L+A%3BAurbach%2C+G+D&rft.aulast=Fitzpatrick&rft.aufirst=L&rft.date=1988-01-01&rft.volume=252&rft.issue=&rft.spage=47&rft.isbn=&rft.btitle=&rft.title=Progress+in+clinical+and+biological+research&rft.issn=03617742&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-08 N1 - Date created - 1988-04-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Pathological and biochemical effects of dimethyl hydrogen phosphite in Fischer 344 rats. AN - 78135935; 2832231 AB - In a chronic study by the National Toxicology Program (NTP), dimethyl hydrogen phosphite (DMHP) caused neoplastic and nonneoplastic changes in the lungs and forestomach of F344/N rats following gavage administration for 2 years. The current investigation was designed to study the effect of a short-term exposure on a series of biochemical systems in target and nontarget tissues which may be involved in the metabolism and/or the manifestation of DMHP toxicity. Rats were treated daily with a dose similar to that used in the NTP study (200 mg/kg) for 4, 5, or 6 weeks. Two groups of animals were also treated for 4 weeks and then treatment was discontinued and the rats were allowed to recover for 1 or 2 weeks. An equal number of animals was treated similarly with the vehicle and used as control. The microsomal and soluble fractions were separated from liver, lungs, kidneys, forestomach, and glandular stomach from the 6-week treatment group. Another group of rats treated for 6 weeks was prepared for pathology examination of the lungs, forestomach, and glandular stomach. There was a significant increase in the weight of the forestomach of rats treated for 4, 5, or 6 weeks relative to control animals, while no significant difference was observed in the weight of liver, lungs, kidneys, and glandular stomach. The forestomach weight of rats treated for 4 weeks returned to the control value after 1 week of recovery. Microscopic examination of the forestomach of rats treated for 6 weeks revealed a thickened stratified squamous epithelium characterized by hyperplasia, hyperkeratosis, and subepithelial inflammation and edema. There were no microscopic changes in the lungs or glandular stomach of animals treated for 6 weeks. The activity of angiotensin converting enzyme in the serum of rats treated for 4, 5, or 6 weeks was significantly increased over that of control animals. The activity of this enzyme returned to near levels seen in the control animals after 1 week of recovery following 4 weeks of treatment. No treatment-related effect was observed in the activities of the microsomal p-nitroanisole demethylase, soluble glutathione S-transferase, and soluble superoxide dismutase in the five tissues studied. There was a significant increase in the level of nonprotein soluble sulfhydryls in the forestomach but in no other tissue of rats treated for 6 weeks. Also the activity of soluble carboxylesterase was significantly reduced in the lungs and forestomach, but not in any other tissue of the 6-week-treated rats.(ABSTRACT TRUNCATED AT 400 WORDS) JF - Fundamental and applied toxicology : official journal of the Society of Toxicology AU - Nomeir, A A AU - Uraih, L C AD - Toxicology Research and Testing Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 114 EP - 124 VL - 10 IS - 1 SN - 0272-0590, 0272-0590 KW - Carcinogens KW - 0 KW - Organophosphonates KW - Organophosphorus Compounds KW - Phosphites KW - Sulfhydryl Compounds KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Nitroanisole O-Demethylase KW - EC 1.- KW - Carboxylic Ester Hydrolases KW - EC 3.1.1.- KW - Peptidyl-Dipeptidase A KW - EC 3.4.15.1 KW - dimethyl hydrogen phosphite KW - ST4TBO000H KW - Index Medicus KW - Animals KW - Carboxylic Ester Hydrolases -- metabolism KW - Sulfhydryl Compounds -- metabolism KW - Cytochrome P-450 Enzyme System -- metabolism KW - Rats KW - Rats, Inbred F344 KW - Nitroanisole O-Demethylase -- metabolism KW - Stomach -- metabolism KW - Peptidyl-Dipeptidase A -- metabolism KW - Male KW - Stomach -- enzymology KW - Organ Size -- drug effects KW - Organophosphorus Compounds -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78135935?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.atitle=Pathological+and+biochemical+effects+of+dimethyl+hydrogen+phosphite+in+Fischer+344+rats.&rft.au=Nomeir%2C+A+A%3BUraih%2C+L+C&rft.aulast=Nomeir&rft.aufirst=A&rft.date=1988-01-01&rft.volume=10&rft.issue=1&rft.spage=114&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-26 N1 - Date created - 1988-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Potential metabolic basis for enflurane hepatitis and the apparent cross-sensitization between enflurane and halothane. AN - 78133819; 2894942 AB - Clinical case reports of unexplained hepatic dysfunction following enflurane and isoflurane anesthesia led to the hypothesis that oxidative metabolism of these drugs by cytochromes P-450 produces immunoreactive, covalently bound acylated protein adducts similar to those implicated in the genesis of halothane-induced hepatic necrosis. Microsomal adducts were detected by enzyme-linked immunosorbent assay and immunoblotting techniques utilizing specific anti-trifluoroacetyl (TFA) IgG hapten antibodies in rat liver following enflurane, isoflurane, or halothane administration. Preincubation of the antibodies with microsomes from halothane-pretreated rats or with 500 microM TFA-lysine, markedly inhibited adduct recognition, while preincubation with 500 microM acetyllysine had no effect. The relative amounts of immunoreactive protein adducts formed were halothane much greater than enflurane much greater than isoflurane and correlates directly with the relative extents of metabolism of these agents. These results support the view that acyl metabolites of the volatile anesthetics may become covalently bound to hepatic proteins, thus serving as antigens, and thereby account for the apparent cross-sensitization and idiosyncratic hepatotoxicity reported for these drugs. JF - Drug metabolism and disposition: the biological fate of chemicals AU - Christ, D D AU - Satoh, H AU - Kenna, J G AU - Pohl, L R AD - Laboratory of Chemical Pharmacology, National Heart, Lung, and Blood Institute, Bethesda, MD 20892. PY - 1988 SP - 135 EP - 140 VL - 16 IS - 1 SN - 0090-9556, 0090-9556 KW - Immunoglobulin G KW - 0 KW - Sodium Dodecyl Sulfate KW - 368GB5141J KW - Enflurane KW - 91I69L5AY5 KW - Isoflurane KW - CYS9AKD70P KW - Halothane KW - UQT9G45D1P KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Immunoglobulin G -- analysis KW - Isoflurane -- metabolism KW - Electrophoresis, Polyacrylamide Gel KW - Biotransformation KW - Microsomes, Liver -- metabolism KW - Enzyme-Linked Immunosorbent Assay KW - Acylation KW - Male KW - Enflurane -- metabolism KW - Chemical and Drug Induced Liver Injury -- etiology KW - Halothane -- toxicity KW - Enflurane -- toxicity KW - Chemical and Drug Induced Liver Injury -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78133819?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.atitle=Potential+metabolic+basis+for+enflurane+hepatitis+and+the+apparent+cross-sensitization+between+enflurane+and+halothane.&rft.au=Christ%2C+D+D%3BSatoh%2C+H%3BKenna%2C+J+G%3BPohl%2C+L+R&rft.aulast=Christ&rft.aufirst=D&rft.date=1988-01-01&rft.volume=16&rft.issue=1&rft.spage=135&rft.isbn=&rft.btitle=&rft.title=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-27 N1 - Date created - 1988-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Development of a testing battery to assess chemical-induced immunotoxicity: National Toxicology Program's guidelines for immunotoxicity evaluation in mice. AN - 78132010; 3280374 JF - Fundamental and applied toxicology : official journal of the Society of Toxicology AU - Luster, M I AU - Munson, A E AU - Thomas, P T AU - Holsapple, M P AU - Fenters, J D AU - White, K L AU - Lauer, L D AU - Germolec, D R AU - Rosenthal, G J AU - Dean, J H AD - Systemic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 2 EP - 19 VL - 10 IS - 1 SN - 0272-0590, 0272-0590 KW - Index Medicus KW - Animals KW - Toxicology -- standards KW - Reference Standards KW - Mice KW - Immunity -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78132010?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.atitle=Development+of+a+testing+battery+to+assess+chemical-induced+immunotoxicity%3A+National+Toxicology+Program%27s+guidelines+for+immunotoxicity+evaluation+in+mice.&rft.au=Luster%2C+M+I%3BMunson%2C+A+E%3BThomas%2C+P+T%3BHolsapple%2C+M+P%3BFenters%2C+J+D%3BWhite%2C+K+L%3BLauer%2C+L+D%3BGermolec%2C+D+R%3BRosenthal%2C+G+J%3BDean%2C+J+H&rft.aulast=Luster&rft.aufirst=M&rft.date=1988-01-01&rft.volume=10&rft.issue=1&rft.spage=2&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+applied+toxicology+%3A+official+journal+of+the+Society+of+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-26 N1 - Date created - 1988-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Interactions of the imidazodiazepine Ro 15-4513 with chemical convulsants. AN - 78126904; 3349229 AB - 1. The proconvulsant effects of the imidazodiazepine Ro 15-4513, were investigated in mice by use of intravenous infusion of a variety of convulsant drugs. 2. Dose-response and time course studies of Ro 15-4513 against gamma-aminobutyric acid (GABA) antagonists were performed. On the basis of these studies a maximally effective dose of 5 mg kg-1 was administered 5 min before the determination of seizure thresholds in subsequent experiments. 3. Ro 15-4513 (5 mg kg-1) significantly lowered seizure thresholds to pentylenetetrazole, bicuculline and the convulsant benzodiazepine Ro 5-3663, but failed to alter seizure thresholds to picrotoxin, strychnine, caffeine and quipazine. 4. Ro 15-4513 significantly raised seizure threshold to the benzodiazepine receptor inverse agonist methyl 6,7-dimethoxy-4 ethyl-beta-carboline-3-carboxylate (DMCM). 5. These results are discussed in relation to other studies investigating the proconvulsant and alcohol-antagonizing effects of Ro 15-4513. JF - British journal of pharmacology AU - Lister, R G AU - Nutt, D J AD - Laboratory of Clinical Studies, DICBR, NIAAA, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 210 EP - 214 VL - 93 IS - 1 SN - 0007-1188, 0007-1188 KW - Azides KW - 0 KW - Benzodiazepinones KW - Carbolines KW - Convulsants KW - Picrotoxin KW - 124-87-8 KW - Benzodiazepines KW - 12794-10-4 KW - methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate KW - 1309288N1J KW - Ro 5-3663 KW - 70656-87-0 KW - Ro 15-4513 KW - 91917-65-6 KW - Pentylenetetrazole KW - WM5Z385K7T KW - Bicuculline KW - Y37615DVKC KW - Index Medicus KW - Seizures -- chemically induced KW - Animals KW - Drug Interactions KW - Benzodiazepinones -- antagonists & inhibitors KW - Picrotoxin -- antagonists & inhibitors KW - Seizures -- physiopathology KW - Mice KW - Bicuculline -- antagonists & inhibitors KW - Pentylenetetrazole -- antagonists & inhibitors KW - Male KW - Carbolines -- antagonists & inhibitors KW - Convulsants -- pharmacology KW - Azides -- pharmacology KW - Benzodiazepines -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78126904?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+journal+of+pharmacology&rft.atitle=Interactions+of+the+imidazodiazepine+Ro+15-4513+with+chemical+convulsants.&rft.au=Lister%2C+R+G%3BNutt%2C+D+J&rft.aulast=Lister&rft.aufirst=R&rft.date=1988-01-01&rft.volume=93&rft.issue=1&rft.spage=210&rft.isbn=&rft.btitle=&rft.title=British+journal+of+pharmacology&rft.issn=00071188&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-28 N1 - Date created - 1988-04-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 1974 Jun;71(6):2246-50 [4152296] Science. 1986 Dec 5;234(4781):1243-7 [3022383] Neuropharmacology. 1980 Oct;19(10):1017-23 [7422073] Brain Res. 1981 Jul 20;216(2):399-408 [6113878] Eur J Pharmacol. 1981 May 8;71(2-3):287-95 [6113969] J Neurochem. 1981 Jul;37(1):1-13 [6265597] Proc Natl Acad Sci U S A. 1981 May;78(5):3260-4 [6265942] Eur J Pharmacol. 1982 Jun 4;80(4):347-58 [6286323] Psychopharmacology (Berl). 1982;78(3):293-5 [6296905] Eur J Pharmacol. 1983 Jan 28;87(1):155-8 [6301850] J Neurochem. 1984 Jan;42(1):221-9 [6689688] Brain Res. 1984 Jan 2;290(1):183-6 [6419985] Eur J Pharmacol. 1984 Jan 27;97(3-4):289-93 [6423394] Eur J Pharmacol. 1984 Aug 17;103(3-4):359-62 [6092108] Br J Pharmacol. 1984 Dec;83(4):951-8 [6097329] Br J Pharmacol. 1985 Oct;86(2):465-73 [2932195] Eur J Pharmacol. 1986 Mar 18;122(2):161-5 [2872063] J Pharm Pharmacol. 1986 Sep;38(9):697-8 [2877073] Neuropharmacology. 1976 Mar;15(3):173-9 [132619] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Control of smoking in occupational epidemiologic studies: methods and needs. AN - 78124197; 3344754 JF - American journal of industrial medicine AU - Blair, A AU - Steenland, K AU - Shy, C AU - O'Berg, M AU - Halperin, W AU - Thomas, T AD - Occupational Studies Section, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 3 EP - 4 VL - 13 IS - 1 SN - 0271-3586, 0271-3586 KW - Index Medicus KW - Risk Factors KW - Humans KW - Epidemiologic Methods KW - Occupational Diseases -- etiology KW - Smoking -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78124197?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Control+of+smoking+in+occupational+epidemiologic+studies%3A+methods+and+needs.&rft.au=Blair%2C+A%3BSteenland%2C+K%3BShy%2C+C%3BO%27Berg%2C+M%3BHalperin%2C+W%3BThomas%2C+T&rft.aulast=Blair&rft.aufirst=A&rft.date=1988-01-01&rft.volume=13&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-25 N1 - Date created - 1988-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Indirect corrections for confounding under multiplicative and additive risk models. AN - 78124158; 3344751 AB - We define a multiplicative model and an additive model for the hazards associated jointly with exposure and with the presence of a confounder like smoking. Under the multiplicative model, the crude relative risk may be adjusted indirectly, by means of a factor proposed by Axelson [1978], and implicitly by Cornfield et al. [1959] and Schlesselman [1978]. We present corresponding indirect correction formulas under the additive risk model for the risk difference and for the excess relative risk. Conditions are established under which these corrections may be applied to age-adjusted rates from composite study populations. We demonstrate that indirect corrections may be no better than crude measures of risk if one assumes the wrong model for the joint action of the exposure and confounding factors. These results are illustrated on an example of occupational exposure to vermiculite. The limitations of the techniques are discussed. JF - American journal of industrial medicine AU - Gail, M H AU - Wacholder, S AU - Lubin, J H AD - Epidemiologic Methods Section, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 119 EP - 130 VL - 13 IS - 1 SN - 0271-3586, 0271-3586 KW - Index Medicus KW - Risk Factors KW - Humans KW - Lung Neoplasms -- mortality KW - Models, Theoretical KW - Epidemiologic Methods KW - Occupational Diseases -- etiology KW - Smoking -- epidemiology KW - Mathematics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78124158?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Indirect+corrections+for+confounding+under+multiplicative+and+additive+risk+models.&rft.au=Gail%2C+M+H%3BWacholder%2C+S%3BLubin%2C+J+H&rft.aulast=Gail&rft.aufirst=M&rft.date=1988-01-01&rft.volume=13&rft.issue=1&rft.spage=119&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-25 N1 - Date created - 1988-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Relative risk models for assessing the joint effects of multiple factors. AN - 78123298; 3344753 AB - A goal of analyses of occupational cohort data is the specification of how covariate information relates to age-specific disease risks. In describing this relationship, certain assumptions or models must be defined. For example, the usual standardized mortality ratio assumes a constant multiplicative increase in the age and calendar period disease rates of an exposed cohort over rates in a unexposed referent group. For analyzing several exposures, some of which may be continuous, such as cumulative dose, dose rate, duration of employment, and smoking patterns, or for analyzing complex associations between disease rate and covariates, flexible regression procedures are required. Using a crossclassification of the data and a Poisson probability model, relative risk regression methods are outlined. Breslow and Storer [1985], Guerrero and Johnson [1982], and Thomas [1981] propose general models for the relative risk as alternatives to, but which include, the usual exponential form. We review these models, discuss some limitations (in particular when there is more than one covariate) and present alternatives. Methods and models are illustrated by examining the joint effects of radon exposure and tobacco use on lung cancer mortality among a group of uranium miners. JF - American journal of industrial medicine AU - Lubin, J H AU - Gaffey, W AD - Biostatics Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 149 EP - 167 VL - 13 IS - 1 SN - 0271-3586, 0271-3586 KW - Uranium KW - 4OC371KSTK KW - Radon KW - Q74S4N8N1G KW - Index Medicus KW - Age Factors KW - Humans KW - Aged KW - Radon -- adverse effects KW - Smoking -- epidemiology KW - Lung Neoplasms -- etiology KW - Aged, 80 and over KW - Risk Factors KW - Adult KW - Neoplasms, Radiation-Induced KW - Middle Aged KW - Mining KW - Models, Theoretical KW - Epidemiologic Methods KW - Statistics as Topic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78123298?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+industrial+medicine&rft.atitle=Relative+risk+models+for+assessing+the+joint+effects+of+multiple+factors.&rft.au=Lubin%2C+J+H%3BGaffey%2C+W&rft.aulast=Lubin&rft.aufirst=J&rft.date=1988-01-01&rft.volume=13&rft.issue=1&rft.spage=149&rft.isbn=&rft.btitle=&rft.title=American+journal+of+industrial+medicine&rft.issn=02713586&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-25 N1 - Date created - 1988-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Salmonella mutagenicity tests: IV. Results from the testing of 300 chemicals. AN - 78120865; 3277844 AB - Three hundred chemicals were tested for mutagenicity, under code, in Salmonella typhimurium, using a preincubation protocol. All tests were performed in the absence of exogenous metabolic activation, and in the presence of liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters. The results and data from these tests are presented. JF - Environmental and molecular mutagenesis AU - Zeiger, E AU - Anderson, B AU - Haworth, S AU - Lawlor, T AU - Mortelmans, K AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 157 VL - 11 Suppl 12 SN - 0893-6692, 0893-6692 KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Chemistry KW - Biotransformation KW - Microsomes, Liver -- metabolism KW - Chemical Phenomena KW - Mesocricetus KW - Male KW - Cricetinae KW - Mutagenicity Tests KW - Salmonella typhimurium -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78120865?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+and+molecular+mutagenesis&rft.atitle=Salmonella+mutagenicity+tests%3A+IV.+Results+from+the+testing+of+300+chemicals.&rft.au=Zeiger%2C+E%3BAnderson%2C+B%3BHaworth%2C+S%3BLawlor%2C+T%3BMortelmans%2C+K&rft.aulast=Zeiger&rft.aufirst=E&rft.date=1988-01-01&rft.volume=11+Suppl+12&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Environmental+and+molecular+mutagenesis&rft.issn=08936692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-28 N1 - Date created - 1988-03-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Pulmonary macrophages are attracted to inhaled particles through complement activation. AN - 78120748; 2830106 AB - Pulmonary macrophages play a central role in clearing inhaled particles from the lung. Previously, we showed that inhaled asbestos fibers activate complement-dependent chemotactic factors on alveolar surfaces to facilitate macrophage recruitment to sites of fiber deposition. In the studies presented here, we have tested a variety of inorganic particles for complement activation in vitro and correlated these data with results on particle-induced macrophage accumulation in vivo. We found that significant chemotactic activity was activated in rat serum and concentrated lavaged proteins by chrysotile and crocidolite asbestos, iron-coated chrysotile asbestos, fiberglass, and wollastonite fibers, as well as by carbonyl iron and zymosan particles. Ash from the Mt. St. Helens volcano did not induce chemotactic activity in either the serum or lavaged proteins. Rats were exposed to brief aerosols of each of the particles listed above (except zymosan). All the particle types studied were deposited primarily at first alveolar duct bifurcations. In addition, all of the particles, except Mt. St. Helens ash, induced at 48 h postexposure significant accumulations of macrophages at these sites. Time-course studies of carbonyl iron particle exposure demonstrated that iron induced a rapid macrophage response, but both particles and phagocytic macrophages were cleared from alveolar surfaces within 8 days after exposure. The Mt. St. Helens ash induced no macrophage accumulation at any time postexposure. We conclude that particles with a wide variety of physical characteristics are capable of activating complement and consequently attracting macrophages, both in vitro and in vivo. We suggest that complement activation is a mechanism through which pulmonary macrophages can detect inhaled particles on alveolar surfaces. JF - Experimental lung research AU - Warheit, D B AU - Overby, L H AU - George, G AU - Brody, A R AD - Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988 PY - 1988 DA - 1988 SP - 51 EP - 66 VL - 14 IS - 1 SN - 0190-2148, 0190-2148 KW - Asbestos, Serpentine KW - 0 KW - Calcium Compounds KW - Chemotactic Factors KW - Dust KW - Silicates KW - fiberglass KW - Asbestos, Crocidolite KW - 12001-28-4 KW - Asbestos KW - 1332-21-4 KW - Silicic Acid KW - 1343-98-2 KW - calcium silicate KW - S4255P4G5M KW - Index Medicus KW - Animals KW - Bronchial Provocation Tests KW - Asbestos -- pharmacology KW - Chemotaxis KW - Glass -- pharmacology KW - Silicic Acid -- metabolism KW - Rats KW - Silicic Acid -- pharmacology KW - Glass -- metabolism KW - Phagocytosis KW - Asbestos -- metabolism KW - Male KW - Macrophages -- cytology KW - Chemotactic Factors -- physiology KW - Pulmonary Alveoli -- cytology KW - Macrophages -- physiology KW - Complement Activation -- drug effects KW - Macrophages -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78120748?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+lung+research&rft.atitle=Pulmonary+macrophages+are+attracted+to+inhaled+particles+through+complement+activation.&rft.au=Warheit%2C+D+B%3BOverby%2C+L+H%3BGeorge%2C+G%3BBrody%2C+A+R&rft.aulast=Warheit&rft.aufirst=D&rft.date=1988-01-01&rft.volume=14&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Experimental+lung+research&rft.issn=01902148&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-07 N1 - Date created - 1988-04-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Carcinogenesis in rats by cyclic N-nitrosamines containing sulphur. AN - 78117363; 3345967 AB - The effects of chronic exposure to three sulphur-containing heterocyclic N-nitrosamines were determined after repeated oral administration to female Fischer 344 rats. Nitrosothiazolidine did not significantly affect the survival of the rats or the incidence of tumours at a total dose of 3.5 mmol. Nitrosodithiazine, an analogue of nitrosothiazolidine which contains an extra sulphur atom inserted between the carbons of its CH2-CH2 moiety, produced only three tumours (two of the nasal mucosa) in a group of 20 rats at a total dose of 1.75 mmol/rat. Nitrosothialdine, the all-cis 2,4,6-trimethyl analogue of nitrosodithiazine, was a potent carcinogen that significantly shortened the lifespan and produced oesophageal tumours in 70% of treated rats as well as numerous tumours of the tongue and liver; this outcome was unexpected because alpha-methyl substitution in other heterocyclic nitrosamines usually reduces or eliminates tumorigenicity. The results extend the data base on the carcinogenic activity of molecules containing both divalent sulphur and the nitrosamino function. The lack of significant carcinogenicity of nitrosothiazolidine in this study suggests that its presence in the human food supply presents a relatively minor risk. JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association AU - Lijinsky, W AU - Kovatch, R M AU - Keefer, L K AU - Saavedra, J E AU - Hansen, T J AU - Miller, A J AU - Fiddler, W AD - NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, MD 21701. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 3 EP - 7 VL - 26 IS - 1 SN - 0278-6915, 0278-6915 KW - Heterocyclic Compounds KW - 0 KW - Nitrosamines KW - Nitroso Compounds KW - Thiazoles KW - N-nitrosodithiazine KW - 114282-83-6 KW - N-nitrosothiazolidine KW - 73870-33-4 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Mutagenicity Tests KW - Chemistry KW - Chemical Phenomena KW - Female KW - Structure-Activity Relationship UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78117363?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Food+and+chemical+toxicology+%3A+an+international+journal+published+for+the+British+Industrial+Biological+Research+Association&rft.atitle=Carcinogenesis+in+rats+by+cyclic+N-nitrosamines+containing+sulphur.&rft.au=Lijinsky%2C+W%3BKovatch%2C+R+M%3BKeefer%2C+L+K%3BSaavedra%2C+J+E%3BHansen%2C+T+J%3BMiller%2C+A+J%3BFiddler%2C+W&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=26&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Food+and+chemical+toxicology+%3A+an+international+journal+published+for+the+British+Industrial+Biological+Research+Association&rft.issn=02786915&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-13 N1 - Date created - 1988-04-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cocaine produces fine structural nuclear alterations in cultured neuroglioblastoma cells. AN - 78117146; 3342342 AB - NG 108-15 neuroglioblastoma cells were grown in culture medium containing either 10(-3), 10(-6) or 10(-9) M cocaine for 1-3 days. Some cultures were assessed for viability while others were processed for electron microscopy. Following 1-3 days of cocaine, no cytoplasmic alterations were observed compared to control; however, numerous dense bodies were present in some cells cultured with the highest doses. After 2 days of treatment with 10(-3) and 10(-6) M cocaine, nuclear invaginations were found filled with vesicles, and the nuclear membrane surrounding the vesicles was disrupted. Following 3 days of treatment with 10(-3) and 10(-6) M cocaine, patches of vesicles and tubules were also seen in the nucleus, but without surrounding membranes. The vesicles ranged in size from 0.05 to 0.8 micron. Cell counts revealed a significant slowing in the rate of cell division after two days of exposure to 10(-3) M cocaine. All concentrations of cocaine caused a significant decrease in cell viability by the third day of treatment. These results suggest that cocaine may interfere with cell replication and also may have a neurotoxic effect. JF - Brain research bulletin AU - Johnson, J E AU - Weissman, A D AD - Neuropharmacology Laboratory, National Institute on Drug Abuse, Baltimore, MD 21224. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 39 EP - 47 VL - 20 IS - 1 SN - 0361-9230, 0361-9230 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Animals KW - Microscopy, Electron KW - Glioma KW - Neuroblastoma KW - Cell Line KW - Cell Nucleus -- ultrastructure KW - Neurons -- drug effects KW - Cell Nucleus -- drug effects KW - Cocaine -- pharmacology KW - Neurons -- ultrastructure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78117146?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research+bulletin&rft.atitle=Cocaine+produces+fine+structural+nuclear+alterations+in+cultured+neuroglioblastoma+cells.&rft.au=Johnson%2C+J+E%3BWeissman%2C+A+D&rft.aulast=Johnson&rft.aufirst=J&rft.date=1988-01-01&rft.volume=20&rft.issue=1&rft.spage=39&rft.isbn=&rft.btitle=&rft.title=Brain+research+bulletin&rft.issn=03619230&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-05 N1 - Date created - 1988-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - In vivo generation of lymphokine activated killer cell activity by ABPP and interleukin-2 and their antitumor effects against immunogenic and nonimmunogenic tumors in murine tumor models. AN - 78116500; 3257900 AB - The capacity of the interferon inducer ABPP and recombinant interleukin-2 (IL-2) to generate lymphokine activated killer (LAK) cell activity in vivo was examined and compared to the cytolysis of fresh tumor cells by in vitro generated LAK cells. Various tumors differing in histology and immunogenicity were used in vitro and in vivo experiments. The i.p. administration of ABPP or IL-2 generated much higher levels of LAK cell activity in the peritoneal exudate than in the spleen. Administration of 2 injections of ABPP was as effective as a 3-day course of moderate doses of IL-2. Generation of LAK cell activity by IL-2 was dose dependent. ABPP had significant antitumor activity in vivo in both the i.p. tumor model and the pulmonary metastasis model when administered early (24-48 h after tumor inoculation), but was ineffective against established (day 3) tumor or advanced grossly visible i.p. (day 8) tumor. Treatment of established tumor with IL-2 and LAK cells was not more effective when ABPP was given concurrently. In contrast when ABPP preceded IL-2 and LAK treatment an additional antitumor effect was seen. Immunogenic tumors were more sensitive to treatment with ABPP than nonimmunogenic tumors. Only a marginal difference in lysability in vitro existed. The antitumor effects of ABPP in vivo may therefore be mediated by mechanisms other than cytolysis by activated killer cells alone. These data taken together suggest that ABPP and IL-2 induce discernable levels of LAK cell activity, but do not synergize when combined. JF - Cancer immunology, immunotherapy : CII AU - Eggermont, A M AU - Sugarbaker, P H AU - Marquet, R L AU - Jeekel, J AD - Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 23 EP - 30 VL - 26 IS - 1 SN - 0340-7004, 0340-7004 KW - Interleukin-2 KW - 0 KW - Cytosine KW - 8J337D1HZY KW - bropirimine KW - J57CTF25XJ KW - Index Medicus KW - Ascitic Fluid -- cytology KW - Animals KW - Spleen -- cytology KW - Ascitic Fluid -- immunology KW - Mice, Inbred C57BL KW - Spleen -- immunology KW - Mice KW - Drug Synergism KW - Female KW - Interleukin-2 -- pharmacology KW - Killer Cells, Natural -- transplantation KW - Neoplasms, Experimental -- immunology KW - Cytosine -- pharmacology KW - Neoplasms, Experimental -- therapy KW - Cytosine -- analogs & derivatives KW - Killer Cells, Natural -- immunology KW - Killer Cells, Natural -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78116500?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+immunology%2C+immunotherapy+%3A+CII&rft.atitle=In+vivo+generation+of+lymphokine+activated+killer+cell+activity+by+ABPP+and+interleukin-2+and+their+antitumor+effects+against+immunogenic+and+nonimmunogenic+tumors+in+murine+tumor+models.&rft.au=Eggermont%2C+A+M%3BSugarbaker%2C+P+H%3BMarquet%2C+R+L%3BJeekel%2C+J&rft.aulast=Eggermont&rft.aufirst=A&rft.date=1988-01-01&rft.volume=26&rft.issue=1&rft.spage=23&rft.isbn=&rft.btitle=&rft.title=Cancer+immunology%2C+immunotherapy+%3A+CII&rft.issn=03407004&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-15 N1 - Date created - 1988-04-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - An endo-exonuclease activity of yeast that requires a functional RAD52 gene. AN - 78112664; 2830467 AB - Extracts of Rad+ and radiation-sensitive (rad) mutants of the yeast Saccharomyces cerevisiae were examined for total Mg2+-dependent alkaline deoxyribonuclease activity and the presence of a nuclease that crossreacts immunologically with an antiserum raised against an endo-exonuclease from Neurospora crassa, an enzyme exhibiting both deoxyribo- and ribonuclease activities. No significant differences were observed in total deoxyribonuclease activity between Rad+ and rad mutants. The antibody precipitable activity, however, was found to be 30%-40% of the total alkaline deoxyribonuclease activity in logarithmically growing Rad+ cells. Extracts of stationary phase cells were lacking in antibody precipitable activity. Using immunoblot methods, a 72 kDa crossreacting protein was identified from logarithmically growing cells that was absent from stationary phase cells. In all radiation-sensitive mutants examined, except rad52, at least 20% of total activity was precipitable. Extracts from logarithmically growing rad52 mutants, including a rad52::LEU2 insertion mutant, exhibited less than 10% of the Rad+ precipitable activity; however, some crossreacting material was detected. Although, the level of endo-exonuclease activity is influenced by the RAD52 gene, it is not the product of this gene. The total deoxyribonuclease and the antibody precipitable endo-exonuclease activities were also followed during meiosis. Unlike the Rad+ strain which had previously been shown to have increased levels of total and immunoprecipitable endo-exonuclease as cells underwent meiosis, the rad52 mutant exhibited no increases in either category of nuclease activity. Given the importance of the RAD52 gene in repair, recombination and mutagenesis, the endo-exonuclease may be a significant component of these processes. JF - Molecular & general genetics : MGG AU - Chow, T Y AU - Resnick, M A AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 41 EP - 48 VL - 211 IS - 1 SN - 0026-8925, 0026-8925 KW - Fungal Proteins KW - 0 KW - Endonucleases KW - EC 3.1.- KW - Exonucleases KW - endoexonuclease KW - EC 3.1.4.- KW - Index Medicus KW - Genes, Fungal KW - Meiosis KW - Haploidy KW - Recombination, Genetic KW - Immunologic Techniques KW - Fungal Proteins -- genetics KW - Diploidy KW - DNA Repair KW - Exonucleases -- genetics KW - Saccharomyces cerevisiae -- enzymology KW - Endonucleases -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78112664?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+%26+general+genetics+%3A+MGG&rft.atitle=An+endo-exonuclease+activity+of+yeast+that+requires+a+functional+RAD52+gene.&rft.au=Chow%2C+T+Y%3BResnick%2C+M+A&rft.aulast=Chow&rft.aufirst=T&rft.date=1988-01-01&rft.volume=211&rft.issue=1&rft.spage=41&rft.isbn=&rft.btitle=&rft.title=Molecular+%26+general+genetics+%3A+MGG&rft.issn=00268925&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-05 N1 - Date created - 1988-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Metabolic mapping of the oculomotor system in MPTP-induced parkinsonian monkeys. AN - 78111119; 3257855 AB - The quantitative autoradiographic 2-[14C]deoxyglucose method was used to map the distribution of alterations of local cerebral metabolic rate for glucose (lCMRGlc) in the oculomotor system of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-(MPTP)-induced parkinsonian monkeys. The lCMRGlc was decreased in the frontal eye fields and in the paralamellar mediodorsal thalamus in the parkinsonian monkeys as compared to normal controls. No changes in lCMRGlc were observed in other areas of the oculomotor system. L-Dopa therapy reversed the oculomotor symptoms in these monkeys, as well as the decrease of lCMRGlc in the frontal eye fields and the paralamellar mediodorsal thalamus. Because the frontal eye fields are known to be involved in the initiation of voluntary saccades, these findings suggest a functional anatomical basis for the oculomotor deficits in Parkinson's disease. JF - Annals of neurology AU - Ho, V W AU - Porrino, L J AU - Crane, A M AU - Burns, R S AU - Kopin, I J AU - Sokoloff, L AD - Howard Hughes Medical Institute, National Institutes of Health Research Scholar Program, Bethesda, MD. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 86 EP - 89 VL - 23 IS - 1 SN - 0364-5134, 0364-5134 KW - Pyridines KW - 0 KW - Levodopa KW - 46627O600J KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - 9P21XSP91P KW - Glucose KW - IY9XDZ35W2 KW - Index Medicus KW - Eye Movements -- drug effects KW - Animals KW - Levodopa -- therapeutic use KW - Macaca mulatta KW - Male KW - Female KW - Parkinson Disease, Secondary -- physiopathology KW - Parkinson Disease, Secondary -- metabolism KW - Parkinson Disease, Secondary -- chemically induced KW - Oculomotor Muscles -- physiopathology KW - Glucose -- metabolism KW - Brain -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78111119?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+neurology&rft.atitle=Metabolic+mapping+of+the+oculomotor+system+in+MPTP-induced+parkinsonian+monkeys.&rft.au=Ho%2C+V+W%3BPorrino%2C+L+J%3BCrane%2C+A+M%3BBurns%2C+R+S%3BKopin%2C+I+J%3BSokoloff%2C+L&rft.aulast=Ho&rft.aufirst=V&rft.date=1988-01-01&rft.volume=23&rft.issue=1&rft.spage=86&rft.isbn=&rft.btitle=&rft.title=Annals+of+neurology&rft.issn=03645134&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-25 N1 - Date created - 1988-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The genetic toxicity of human carcinogens and its implications. AN - 78106840; 3277048 AB - 23 chemicals and chemical combinations have been designated by the International Agency for Research on Cancer (IARC) as causally associated with cancer in humans. The literature was searched for reports of their activity in the Salmonella mutagenicity assay and for evidence of their ability to induce chromosome aberrations or micronuclei in the bone marrow of mice or rats. In addition, the chemical structures of these carcinogens were assessed for the presence of electrophilic substituents that might be associated with their mutagenicity and carcinogenicity. The purpose of this study was to determine which human carcinogens exhibit genetic toxicity in vitro and in vivo and to what extent they can be detected using these two widely employed short-term tests for genetic toxicity. The results of this study revealed 20 of the 23 carcinogens to be active in one or both short-term tests. Treosulphan, for which short-term test results are not available, is predicted to be active based on its structure. The remaining two agents, asbestos and conjugated estrogens, are not mutagenic to Salmonella; asbestos is not likely to induce cytogenetic effects in the bone marrow and the potential activity of conjugated estrogens in the bone marrow is difficult to anticipate. These findings show that genetic toxicity is characteristic of the majority of IARC Group 1 human carcinogens. If these chemicals are considered representative of human carcinogens, then two short-term tests may serve as an effective primary screen for chemicals that present a carcinogenic hazard to humans. JF - Mutation research AU - Shelby, M D AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 3 EP - 15 VL - 204 IS - 1 SN - 0027-5107, 0027-5107 KW - Carcinogens KW - 0 KW - Mutagens KW - Index Medicus KW - Animals KW - Risk Factors KW - Humans KW - Structure-Activity Relationship KW - Mutagenicity Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78106840?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+research&rft.atitle=The+genetic+toxicity+of+human+carcinogens+and+its+implications.&rft.au=Shelby%2C+M+D&rft.aulast=Shelby&rft.aufirst=M&rft.date=1988-01-01&rft.volume=204&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Mutation+research&rft.issn=00275107&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-21 N1 - Date created - 1988-03-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Chordomas in Fischer 344 rats. AN - 78106317; 3344569 AB - Tissue sections and records of 56 rats with chordoma, identified in the National Toxicology Program's (NTP) data base of approximately 115,000 rats, were examined to determine morphological characteristics, incidence, and aspects of biological behavior. Chordomas occurred in aged rats, originated predominantly in lumbosacral vertebrae, were highly malignant, occurred three times more often in male versus female rats, and commonly produced bilateral posterior paresis, paralysis, and/or distention of the colon and rectum. JF - Veterinary pathology AU - Stefanski, S A AU - Elwell, M R AU - Mitsumori, K AU - Yoshitomi, K AU - Dittrich, K AU - Giles, H D AD - Chemical Pathology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 42 EP - 47 VL - 25 IS - 1 SN - 0300-9858, 0300-9858 KW - Index Medicus KW - Rats KW - Animals KW - Microscopy, Electron KW - Immunohistochemistry KW - Sacrum KW - Male KW - Female KW - Lumbar Vertebrae KW - Rats, Inbred Strains KW - Chordoma -- pathology KW - Rats, Inbred F344 KW - Skull Neoplasms -- pathology KW - Chordoma -- ultrastructure KW - Spinal Neoplasms -- ultrastructure KW - Spinal Neoplasms -- pathology KW - Chordoma -- veterinary KW - Skull Neoplasms -- veterinary KW - Spinal Neoplasms -- veterinary KW - Skull Neoplasms -- ultrastructure KW - Rodent Diseases -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78106317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Veterinary+pathology&rft.atitle=Chordomas+in+Fischer+344+rats.&rft.au=Stefanski%2C+S+A%3BElwell%2C+M+R%3BMitsumori%2C+K%3BYoshitomi%2C+K%3BDittrich%2C+K%3BGiles%2C+H+D&rft.aulast=Stefanski&rft.aufirst=S&rft.date=1988-01-01&rft.volume=25&rft.issue=1&rft.spage=42&rft.isbn=&rft.btitle=&rft.title=Veterinary+pathology&rft.issn=03009858&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-28 N1 - Date created - 1988-03-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Phorbol esters induce multidrug resistance in human breast cancer cells. AN - 78105437; 3422442 AB - Mechanisms responsible for broad-based resistance to antitumor drugs derived from natural products (multidrug resistance) are incompletely understood. Agents known to reverse the multidrug-resistant phenotype (verapamil and trifluoperazine) can also inhibit the activity of protein kinase C. When we assayed human breast cancer cell lines for protein kinase C activity, we found that enzyme activity was 7-fold higher in the multidrug-resistant cancer cells compared with the control, sensitive parent cells. Exposure of drug-sensitive cells to the phorbol ester phorbol 12,13-dibutyrate [P(BtO)2] led to an increase in protein kinase C activity and induced a drug-resistance phenotype, whereas exposure of drug-resistant cells to P(BtO)2 further increased drug resistance. In sensitive cells, this increased resistance was accompanied by a 3.5-fold increased phosphorylation of a 20-kDa particulate protein and a 35-40% decreased intracellular accumulation of doxorubicin and vincristine. P(BtO)2 induced resistance to agents involved in the multidrug-resistant phenotype (doxorubicin and vincristine) but did not affect sensitivity to an unrelated alkylating agent (melphalan). The increased resistance was partially or fully reversible by the calcium channel blocker verapamil and by the calmodulin-antagonist trifluoperazine. These data suggest that stimulation of protein kinase C plays a role in the drug-transport changes in multidrug-resistant cells. This may occur through modulation of an efflux pump by protein phosphorylation. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Fine, R L AU - Patel, J AU - Chabner, B A AD - Clinical Pharmacology Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 582 EP - 586 VL - 85 IS - 2 SN - 0027-8424, 0027-8424 KW - Antineoplastic Agents KW - 0 KW - Carcinogens KW - Phorbol Esters KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Index Medicus KW - Protein Kinase C -- metabolism KW - Clone Cells KW - Phosphorylation KW - Humans KW - Drug Resistance KW - Female KW - Cell Line KW - Phorbol Esters -- pharmacology KW - Carcinogens -- pharmacology KW - Breast Neoplasms -- pathology KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78105437?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Phorbol+esters+induce+multidrug+resistance+in+human+breast+cancer+cells.&rft.au=Fine%2C+R+L%3BPatel%2C+J%3BChabner%2C+B+A&rft.aulast=Fine&rft.aufirst=R&rft.date=1988-01-01&rft.volume=85&rft.issue=2&rft.spage=582&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-03 N1 - Date created - 1988-03-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nature. 1970 Aug 15;227(5259):680-5 [5432063] Proc Natl Acad Sci U S A. 1986 Dec;83(24):9328-32 [3540935] Biochim Biophys Acta. 1977 Apr 25;491(2):387-97 [857903] J Biol Chem. 1980 Sep 25;255(18):8378-80 [7410368] Biochem Biophys Res Commun. 1981 Mar 16;99(1):228-35 [7236262] J Biol Chem. 1982 Jul 25;257(14):8489-95 [7085679] Cancer Res. 1983 May;43(5):2267-72 [6831450] Cancer Res. 1983 Jun;43(6):2905-10 [6850602] Proc Natl Acad Sci U S A. 1983 Dec;80(23):7244-8 [6316349] Cancer Res. 1984 Jan;44(1):144-8 [6690032] Science. 1984 Jun 1;224(4652):994-6 [6372095] Cancer Res. 1985 Jun;45(6):2462-5 [3157445] J Biol Chem. 1985 Jul 5;260(13):8026-31 [2989273] Leuk Res. 1985;9(7):885-95 [2991669] Biochem Pharmacol. 1986 Feb 1;35(3):467-72 [3947382] Biochem Biophys Res Commun. 1986 Mar 13;135(2):397-402 [3457562] Science. 1986 Jul 18;233(4761):305-12 [3014651] Biochem Pharmacol. 1986 Aug 15;35(16):2683-6 [3461788] Leuk Res. 1986;10(9):1063-9 [3762216] J Biol Chem. 1986 Nov 25;261(33):15544-9 [3782078] Cancer Res. 1987 Jan 15;47(2):433-41 [3791232] Anal Biochem. 1976 May 7;72:248-54 [942051] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Relationship between in vitro tumor stem cell assay and in vivo antitumor activity using the P388 leukemia. AN - 78103391; 3339266 AB - The relationship between in vitro tumor stem cell sensitivity and in vivo antitumor efficacy using 13 active antineoplastic agents was examined by means of the intraperitoneal P388 mouse leukemia system. The doses, which produced a 50% increase in life span after one, five and nine days of treatment, were all significantly correlated with the in vitro concentration producing a 70% reduction in colony formation during a seven-day continuous drug exposure. The five-day correlation was the best, followed by nine days and one day. Correlations were not improved by corrections for either in vitro drug stability or toxicity (LD50). The highly significant correlations observed in this simple retrospective analysis provide a basis for the development of more sophisticated models for the prediction of in vivo results from in vitro data. JF - International journal of cell cloning AU - Marsh, J C AU - Shoemaker, R H AU - Salmon, S E AU - Kern, D H AU - Venditti, J M AD - Developmental Therapeutics Program, National Cancer Institute, Bethesda, Maryland. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 60 EP - 68 VL - 6 IS - 1 SN - 0737-1454, 0737-1454 KW - Antineoplastic Agents KW - 0 KW - Index Medicus KW - Injections, Intraperitoneal KW - Animals KW - Drug Administration Schedule KW - Humans KW - Disease Models, Animal KW - Mice KW - Leukemia P388 -- drug therapy KW - Antineoplastic Agents -- administration & dosage KW - Leukemia, Experimental -- drug therapy KW - Leukemia P388 -- pathology KW - Colony-Forming Units Assay KW - Tumor Stem Cell Assay KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78103391?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+cell+cloning&rft.atitle=Relationship+between+in+vitro+tumor+stem+cell+assay+and+in+vivo+antitumor+activity+using+the+P388+leukemia.&rft.au=Marsh%2C+J+C%3BShoemaker%2C+R+H%3BSalmon%2C+S+E%3BKern%2C+D+H%3BVenditti%2C+J+M&rft.aulast=Marsh&rft.aufirst=J&rft.date=1988-01-01&rft.volume=6&rft.issue=1&rft.spage=60&rft.isbn=&rft.btitle=&rft.title=International+journal+of+cell+cloning&rft.issn=07371454&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-02 N1 - Date created - 1988-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Forskolin promotes the development of ethanol tolerance in 6-hydroxydopamine-treated mice. AN - 78099521; 3123833 AB - Partial depletion of brain norepinephrine by 6-hydroxydopamine prevents the development of functional tolerance to ethanol in mice. This blockade of tolerance development was overcome by daily intracerebroventricular injections of forskolin. These results suggest that interaction of norepinephrine with post-synaptic beta-adrenergic receptors, and activation of adenylate cyclase, is important for the development of ethanol tolerance. Interaction of norepinephrine with alpha 1-adrenergic receptors may be less crucial, since treatment with a phorbol ester activator of protein kinase C did not restore the development of tolerance in mice treated with 6-hydroxydopamine. The importance of the beta-adrenergic receptor-coupled adenylate cyclase system for development of ethanol tolerance, in addition to its previously-reported role in long-term potentiation, suggests that this system may influence neuroadaptive processes in general. JF - Life sciences AU - Szabó, G AU - Hoffman, P L AU - Tabakoff, B AD - Section on Receptor Mechanisms, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1988 PY - 1988 DA - 1988 SP - 615 EP - 621 VL - 42 IS - 6 SN - 0024-3205, 0024-3205 KW - Hydroxydopamines KW - 0 KW - Colforsin KW - 1F7A44V6OU KW - Ethanol KW - 3K9958V90M KW - Oxidopamine KW - 8HW4YBZ748 KW - Index Medicus KW - Drug Tolerance KW - Animals KW - Reference Values KW - Mice, Inbred C57BL KW - Mice KW - Male KW - Injections, Intraventricular KW - Colforsin -- pharmacology KW - Ethanol -- pharmacology KW - Cerebral Ventricles -- physiology KW - Hydroxydopamines -- toxicity KW - Colforsin -- administration & dosage KW - Cerebral Ventricles -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78099521?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Forskolin+promotes+the+development+of+ethanol+tolerance+in+6-hydroxydopamine-treated+mice.&rft.au=Szab%C3%B3%2C+G%3BHoffman%2C+P+L%3BTabakoff%2C+B&rft.aulast=Szab%C3%B3&rft.aufirst=G&rft.date=1988-01-01&rft.volume=42&rft.issue=6&rft.spage=615&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-22 N1 - Date created - 1988-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cytogenetic and chemical detection of human exposure to polyhalogenated aromatic hydrocarbons. AN - 78095944; 3338440 AB - Peripheral lymphocytes from Taiwanese women (n = 35) exposed to polychlorinated aromatic hydrocarbons and from matched controls (n = 24) were assessed for the levels of sister chromatid exchanges (SCEs) after a 72-hour incubation of whole blood in the presence or absence of alpha-naphthoflavone (ANF) and for chromosome aberrations after 48 hours of incubation. Serum levels of polychlorinated biphenyl (PCB) congeners were measured for all individuals, and serum levels of several polychlorinated dibenzofurans (PCDFs) were measured for 12 exposed individuals by gas chromatography-mass spectometry. Blood concentrations of total PCBs in the exposed population averaged approximately 15 ppb, whereas mean PCDF values were 14 ppt. Major PCB congeners detected were 2,2' 4,4', 5,5'-hexa CB and 2,2'3,4,4',5-hexa CB. PCDFs detected were primarily 1,2,3,4,7,8,-hexachlorodibenzofuran (10.8 ppt) and 2,3,4,7,8-pentachlorodibenzofuran (2.7 ppt). Average SCE frequencies were 7.61 for controls and 7.30 for exposed individuals when assays were conducted in the absence of ANF, whereas respective values were 8.85 and 10.75 in the presence of ANF. Differences in the level of ANF-induced SCEs between the two populations were highly significant (P less than .001). Moreover, the ANF-induced SCEs were highly correlated with the serum concentrations of total PCBs and of several PCB congeners (P less than .001). Increases in ANF-induced SCEs appeared to be linear up to a PCB concentration of approximately 30 ppb. Chromosome aberration frequencies were similar in control and exposed populations. These studies demonstrate that in vivo exposure to PCBs and PCDFs result in an enhanced sensitivity of lymphocytes to the SCE-causing actions of ANF. JF - Environmental and molecular mutagenesis AU - Lundgren, K AU - Collman, G W AU - Wang-Wuu, S AU - Tiernan, T AU - Taylor, M AU - Thompson, C L AU - Lucier, G W AD - Laboratory of Biochemical Risk Analysis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. Y1 - 1988 PY - 1988 DA - 1988 SP - 1 EP - 11 VL - 11 IS - 1 SN - 0893-6692, 0893-6692 KW - Benzoflavones KW - 0 KW - Polycyclic Compounds KW - alpha-naphthoflavone KW - 604-59-1 KW - Index Medicus KW - Humans KW - In Vitro Techniques KW - Environmental Exposure KW - Lymphocytes KW - Sister Chromatid Exchange KW - DNA Damage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78095944?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+and+molecular+mutagenesis&rft.atitle=Cytogenetic+and+chemical+detection+of+human+exposure+to+polyhalogenated+aromatic+hydrocarbons.&rft.au=Lundgren%2C+K%3BCollman%2C+G+W%3BWang-Wuu%2C+S%3BTiernan%2C+T%3BTaylor%2C+M%3BThompson%2C+C+L%3BLucier%2C+G+W&rft.aulast=Lundgren&rft.aufirst=K&rft.date=1988-01-01&rft.volume=11&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Environmental+and+molecular+mutagenesis&rft.issn=08936692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-26 N1 - Date created - 1988-02-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Chirality and cytochrome P-450: a perspective. AN - 78094280; 3337733 JF - Biochemical pharmacology AU - Gelboin, H V AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 103 VL - 37 IS - 1 SN - 0006-2952, 0006-2952 KW - Pharmaceutical Preparations KW - 0 KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Index Medicus KW - Animals KW - Pharmaceutical Preparations -- metabolism KW - Humans KW - Molecular Conformation KW - Cytochrome P-450 Enzyme System -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78094280?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+pharmacology&rft.atitle=Chirality+and+cytochrome+P-450%3A+a+perspective.&rft.au=Gelboin%2C+H+V&rft.aulast=Gelboin&rft.aufirst=H&rft.date=1988-01-01&rft.volume=37&rft.issue=1&rft.spage=103&rft.isbn=&rft.btitle=&rft.title=Biochemical+pharmacology&rft.issn=00062952&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-10 N1 - Date created - 1988-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Estrogen receptor-binding activity of polychlorinated hydroxybiphenyls: conformationally restricted structural probes. AN - 78088229; 3122017 AB - A series of polychlorinated hydroxybiphenyls (PCBs) has been tested for their binding activity to soluble uterine estrogen receptor protein. Competitive binding analysis was performed on 0-40% ammonium sulfate-enriched uterine cytosol receptor preparations which improved the binding activity for the PCB compounds by a factor of 10-40, by decreasing the nonspecific binding. The binding activities have been correlated to molecular properties supported by molecular modeling studies which emphasize the importance of conformational restriction. The estrogen receptor bound 4-hydroxy-2',4',6'-trichlorobiphenyl (4H2',4',6'TCB) with the greatest affinity, with the concentration of unlabeled inhibitor yielding half-maximal specific binding relative to estradiol (C50) being approximately 42 compared to estradiol, C50 approximately 1.0. PCB compounds that demonstrated appreciable receptor-binding activity were also active in vivo in stimulating uterine weight increases, whereas weak binders were inactive. The 4H2',4',6'TCB compound represents a high degree of conformational restriction around the interring bond due to the presence of two ortho-chlorine atoms. The other PCBs in this series, which show lower receptor-binding activity, vary in position of chlorine substituents and can assume multiple low energy conformations as a result of less hindrance to rotation around the interring bond. JF - Molecular pharmacology AU - Korach, K S AU - Sarver, P AU - Chae, K AU - McLachlan, J A AU - McKinney, J D AD - Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 120 EP - 126 VL - 33 IS - 1 SN - 0026-895X, 0026-895X KW - Receptors, Estrogen KW - 0 KW - Estradiol KW - 4TI98Z838E KW - Diethylstilbestrol KW - 731DCA35BT KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Animals KW - Uterus KW - Stereoisomerism KW - Diethylstilbestrol -- metabolism KW - Humans KW - Binding, Competitive KW - Cytosol KW - Mice KW - Molecular Conformation KW - Female KW - Structure-Activity Relationship KW - Estradiol -- metabolism KW - Polychlorinated Biphenyls -- metabolism KW - Receptors, Estrogen -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78088229?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+pharmacology&rft.atitle=Estrogen+receptor-binding+activity+of+polychlorinated+hydroxybiphenyls%3A+conformationally+restricted+structural+probes.&rft.au=Korach%2C+K+S%3BSarver%2C+P%3BChae%2C+K%3BMcLachlan%2C+J+A%3BMcKinney%2C+J+D&rft.aulast=Korach&rft.aufirst=K&rft.date=1988-01-01&rft.volume=33&rft.issue=1&rft.spage=120&rft.isbn=&rft.btitle=&rft.title=Molecular+pharmacology&rft.issn=0026895X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-20 N1 - Date created - 1988-02-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Marked disparity in incidence of bacterial infections in patients with the acquired immunodeficiency syndrome receiving interleukin-2 or interferon-gamma. AN - 78082305; 3122616 AB - To compare the infectious complications that occurred during trials of immunomodulatory agents in patients with the acquired immunodeficiency syndrome (AIDS). A survey of two cohorts of patients with AIDS who participated in nonrandomized, unblinded, non-placebo-controlled investigations of the toxicity and efficacy of interleukin-2 and interferon-gamma. Clinical research unit in a tertiary care center. Consecutive samples of 52 patients given interleukin-2 and 22 patients given interferon-gamma. Selection criteria for referred patients included a diagnosis of AIDS, hemoglobin level of greater than 100 g/L (2.0 mg/dL), creatinine level of less than 176.8 mumol/L, bilirubin level of less than 25.65 mumol/L (1.5 mg/dL), the absence of active infection, and the absence of other drug therapy for 2 weeks before entry. Four patients given interleukin-2 failed to complete the study. Intravenous infusion of natural-product or recombinant human interleukin-2, 250 to 10,000,000 U/day for 23.4 +/- 1.5 (SE) days, or recombinant human interferon-gamma, 0.001 to 1.0 mg/m2.d for 17.7 +/- 4.8 days. Twenty nonopportunistic bacterial infections occurred in 17 of 52 patients given interleukin-2, whereas non occurred in 22 patients given interferon-gamma (P less than 0.05). Bacteremia accounted for 12 of the infections. Staphylococcus aureus and gram-negative bacilli accounted for 16 of the isolates. Opportunistic infections occurred in 6 patients during interleukin-2 infusion and in 1 patient during interferon-gamma infusion (P greater than 0.5). Clinical and immunologic variables and methods of management of intravenous catheters were similar in the two groups. A marked disparity in infection with nonopportunistic bacteria, but not with opportunistic organisms, occurred in patients with AIDS who were treated with interleukin-2 as compared with those who were treated with interferon-gamma. A high incidence of bacteremia and localized bacterial infection should be anticipated in patients with AIDS who receive interleukin-2. JF - Annals of internal medicine AU - Murphy, P M AU - Lane, H C AU - Gallin, J I AU - Fauci, A S AD - Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 36 EP - 41 VL - 108 IS - 1 SN - 0003-4819, 0003-4819 KW - Interleukin-2 KW - 0 KW - Interferon-gamma KW - 82115-62-6 KW - Abridged Index Medicus KW - Index Medicus KW - AIDS/HIV KW - Opportunistic Infections -- etiology KW - Immunity, Innate -- drug effects KW - Sepsis -- epidemiology KW - Humans KW - Adult KW - Middle Aged KW - Male KW - Female KW - Acquired Immunodeficiency Syndrome -- therapy KW - Acquired Immunodeficiency Syndrome -- complications KW - Interleukin-2 -- adverse effects KW - Bacterial Infections -- epidemiology KW - Interferon-gamma -- therapeutic use KW - Interleukin-2 -- therapeutic use KW - Bacterial Infections -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78082305?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+internal+medicine&rft.atitle=Marked+disparity+in+incidence+of+bacterial+infections+in+patients+with+the+acquired+immunodeficiency+syndrome+receiving+interleukin-2+or+interferon-gamma.&rft.au=Murphy%2C+P+M%3BLane%2C+H+C%3BGallin%2C+J+I%3BFauci%2C+A+S&rft.aulast=Murphy&rft.aufirst=P&rft.date=1988-01-01&rft.volume=108&rft.issue=1&rft.spage=36&rft.isbn=&rft.btitle=&rft.title=Annals+of+internal+medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-18 N1 - Date created - 1988-02-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Human serum sickness: a prospective analysis of 35 patients treated with equine anti-thymocyte globulin for bone marrow failure. AN - 78081641; 3257288 AB - We have prospectively evaluated the clinical and immunological features of serum sickness in 35 patients treated for bone marrow failure with anti-thymocyte globulin (ATG 15 mg/kg/day) and methylprednisolone (1 to 1.5 mg/kg/day). Twenty-one patients were treated for 10 days and 14 were treated for 28 days. Clinical evidence of serum sickness developed in 30 patients (86%) and included fever and malaise (100%), cutaneous eruptions (93%), arthralgias (67%), gastrointestinal complaints (67%), cephalgia (57%), blurring of vision (37%), arthritis, (30%) and lymphadenopathy (13%). Clinical serum sickness began on day 7 +/- 1 (X +/- S.E.M.) and lasted for 10 +/- 2 days in the 18 affected patients receiving the shorter course of ATG. In the 12 affected patients receiving the longer course of ATG, serum sickness began on day 9 +/- 1. The earliest manifestations of serum sickness were fever, malaise, and cutaneous eruptions. Cutaneous findings consisted of morbilliform eruptions (n = 19) and urticaria (n = 1) or a combination (n = 8) that lasted 10 to 14 days. Twenty-one patients (75%) developed a highly characteristic serpiginous band of erythema and purpura along the sides of the fingers, toes, palms and soles 12 to 48 hours before other symptoms of serum sickness. Biopsies of lesional skin during the course of serum sickness revealed immune deposits (IgM, IgE, IgA and C3) in dermal vasculature in 7 of 9 patients. Immunological changes that occurred during the course of serum sickness included increased serum levels of IgG, IgM, IgA, and IgE. Circulating immune complexes, as measured by the C1q-binding assay, increased from a mean value of 12% to 45% on day 13 +/- 1. Complement levels (C3, C4, and CH50) decreased 50 to 80% from their baseline levels on day 10 +/- 2. Acute phase reactants increased: erythrocyte sedimentation rate, C-reactive protein and beta-2 microglobulin. Abnormal urinalysis developed in 17 patients (57%) over the course of serum sickness and included proteinuria, hematuria and hemoglobinuria on day 10 +/- 3. Hematopoietic response occurred in 43%. All 5 patients who did not develop serum sickness recovered from bone marrow failure. Our data document the clinical and immunopathological findings in human serum sickness and suggest that the principles of antigen-antibody interaction, complement activation, and resultant inflammatory response as seen in the previous animal studies are directly applicable to studies of patients with serum sickness. JF - Medicine AU - Bielory, L AU - Gascon, P AU - Lawley, T J AU - Young, N S AU - Frank, M M AD - Clinical Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 40 EP - 57 VL - 67 IS - 1 SN - 0025-7974, 0025-7974 KW - Antigen-Antibody Complex KW - 0 KW - Antilymphocyte Serum KW - Immunoglobulins KW - Abridged Index Medicus KW - Index Medicus KW - Immunoglobulins -- analysis KW - Prospective Studies KW - Random Allocation KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Child KW - Antigen-Antibody Complex -- analysis KW - Adolescent KW - Hematopoiesis KW - Male KW - Female KW - Serum Sickness -- immunology KW - Antilymphocyte Serum -- adverse effects KW - Anemia, Aplastic -- etiology KW - Bone Marrow -- physiopathology KW - Serum Sickness -- complications KW - T-Lymphocytes -- immunology KW - Serum Sickness -- physiopathology KW - Antilymphocyte Serum -- therapeutic use KW - Anemia, Aplastic -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78081641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Medicine&rft.atitle=Human+serum+sickness%3A+a+prospective+analysis+of+35+patients+treated+with+equine+anti-thymocyte+globulin+for+bone+marrow+failure.&rft.au=Bielory%2C+L%3BGascon%2C+P%3BLawley%2C+T+J%3BYoung%2C+N+S%3BFrank%2C+M+M&rft.aulast=Bielory&rft.aufirst=L&rft.date=1988-01-01&rft.volume=67&rft.issue=1&rft.spage=40&rft.isbn=&rft.btitle=&rft.title=Medicine&rft.issn=00257974&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-10 N1 - Date created - 1988-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - DNA recombinant and monoclonal antibody directed methods for determining cytochrome P-450 specificity. AN - 78079434; 3276324 JF - Biochemical pharmacology AU - Gelboin, H V AU - Park, S S AU - Battula, N AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 98 EP - 102 VL - 37 IS - 1 SN - 0006-2952, 0006-2952 KW - Antibodies, Monoclonal KW - 0 KW - DNA, Recombinant KW - Isoenzymes KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Index Medicus KW - Phenotype KW - Vaccinia virus -- genetics KW - Animals KW - Stereoisomerism KW - Liver -- enzymology KW - Substrate Specificity KW - Immunologic Techniques KW - Species Specificity KW - Cytochrome P-450 Enzyme System -- analysis KW - Isoenzymes -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78079434?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+pharmacology&rft.atitle=DNA+recombinant+and+monoclonal+antibody+directed+methods+for+determining+cytochrome+P-450+specificity.&rft.au=Gelboin%2C+H+V%3BPark%2C+S+S%3BBattula%2C+N&rft.aulast=Gelboin&rft.aufirst=H&rft.date=1988-01-01&rft.volume=37&rft.issue=1&rft.spage=98&rft.isbn=&rft.btitle=&rft.title=Biochemical+pharmacology&rft.issn=00062952&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-10 N1 - Date created - 1988-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Colchicine suppresses the release of fibroblast growth factors from alveolar macrophages in vitro. The basis of a possible therapeutic approach ot the fibrotic disorders. AN - 78077372; 3337460 AB - Fibrosis is the accumulation of fibroblasts and the connective tissue products secreted by these cells, usually subsequent to tissue injury. While fibrosis can be useful in preserving the general structural integrity of a tissue, it often alters cell-cell and cell-connective tissue interactions, which leads to loss of tissue function. On the basis of the concept that mononuclear phagocytes can direct the development of fibrosis through the release of specific mediators that stimulate fibroblast proliferation, we propose a therapeutic strategy to prevent fibrosis by preventing the release of these specific mediators. The present study demonstrated that colchicine, a widely used and well-tolerated drug, can block alveolar macrophage release of 2 mediators associated with the development of fibrosis in interstitial lung diseases, fibronectin, and the alveolar-macrophage-derived growth factor (AMDGF). Colchicine blocked the spontaneous release of fibronectin by alveolar macrophages obtained from patients with fibrotic lung disease by 23 +/- 4% after 24 h and by greater than 90% after 72 h. AMDGF release was blocked by 68 +/- 10% after 4 h (p less than 0.01, all comparisons). The effect of colchicine was not due to nonspecific toxicity since [14C]proline tracer studies demonstrated that macrophages treated with colchicine were capable of de novo protein synthesis and the secretion of several protein products, despite the fact that fibronectin and AMDGF release were suppressed. The effect of colchicine on the spontaneous release of both fibronectin and AMDGF could be observed at concentrations less than 10 ng/ml, levels that can be achieved in vivo.(ABSTRACT TRUNCATED AT 250 WORDS) JF - The American review of respiratory disease AU - Rennard, S I AU - Bitterman, P B AU - Ozaki, T AU - Rom, W N AU - Crystal, R G AD - Pulmonary Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 181 EP - 185 VL - 137 IS - 1 SN - 0003-0805, 0003-0805 KW - Fibronectins KW - 0 KW - Growth Substances KW - Peptides KW - alveolar macrophage growth factor KW - Colchicine KW - SML2Y3J35T KW - Abridged Index Medicus KW - Index Medicus KW - Pulmonary Alveoli -- pathology KW - Dose-Response Relationship, Drug KW - In Vitro Techniques KW - Fibronectins -- secretion KW - Male KW - Female KW - Macrophages -- secretion KW - Colchicine -- pharmacology KW - Growth Substances -- secretion KW - Pulmonary Fibrosis -- drug therapy KW - Colchicine -- therapeutic use KW - Colchicine -- pharmacokinetics KW - Pulmonary Fibrosis -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78077372?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+review+of+respiratory+disease&rft.atitle=Colchicine+suppresses+the+release+of+fibroblast+growth+factors+from+alveolar+macrophages+in+vitro.+The+basis+of+a+possible+therapeutic+approach+ot+the+fibrotic+disorders.&rft.au=Rennard%2C+S+I%3BBitterman%2C+P+B%3BOzaki%2C+T%3BRom%2C+W+N%3BCrystal%2C+R+G&rft.aulast=Rennard&rft.aufirst=S&rft.date=1988-01-01&rft.volume=137&rft.issue=1&rft.spage=181&rft.isbn=&rft.btitle=&rft.title=The+American+review+of+respiratory+disease&rft.issn=00030805&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-10 N1 - Date created - 1988-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Activators of protein kinase C act at a postreceptor site to amplify cyclic AMP production in rat pinealocytes. AN - 78040235; 2447232 AB - Activation of alpha 1-adrenoceptors appears to amplify beta-adrenergic stimulation of cyclic AMP (cAMP) accumulation in rat pinealocytes severalfold by a mechanism involving activation of a Ca2+-, phospholipid-dependent protein kinase (protein kinase C). The mechanism of action of protein kinase C was investigated in this report using intact cells. Activation of protein kinase C with 4 beta-phorbol 12-myristate 13-acetate (PMA; 10(-7) M) or the alpha 1-adrenergic agonist phenylephrine (PE; 10(-6) M) did not inhibit cAMP efflux in beta-adrenergically stimulated cells. The amplification of the beta-adrenergic cAMP response by these agents also occurred in the presence of isobutylmethylxanthine (10(-3) M) and Ro 20-1724 (10(-4) M), an observation suggesting that inhibition of cAMP phosphodiesterase activity is not the mechanism of action. Furthermore, although PMA (10(-7) M) caused a sixfold increase in the magnitude of the cAMP response to isoproterenol, it did not alter the EC50 of the response (1.7 X 10(-8) M), a result indicating that protein kinase C activation does not alter beta-adrenoceptor sensitivity. The cAMP response following cholera toxin pretreatment (60-120 min) was rapidly and markedly enhanced by alpha 1-adrenergic agonists (cirazoline greater than PE greater than methoxamine), by phorbol esters (PMA greater than 4 beta-phorbol 12,13,-dibutyrate much greater than 4 alpha-phorbol 12,13-didecanoate), and by synthetic diacylglycerols (1,2-dioctanoylglycerol greater than 1-oleoyl 2-acetylglycerol much greater than diolein). The cAMP response to forskolin (10(-5)-10(-3) M) was also increased by PE (3 X 10(-6) M) and PMA (10(-7) M).(ABSTRACT TRUNCATED AT 250 WORDS) JF - Journal of neurochemistry AU - Sugden, D AU - Klein, D C AD - Section on Neuroendocrinology, National Institute of Child Health and Human Development, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 149 EP - 155 VL - 50 IS - 1 SN - 0022-3042, 0022-3042 KW - Adrenergic alpha-Agonists KW - 0 KW - Phorbol Esters KW - Receptors, Adrenergic, alpha KW - Receptors, Adrenergic, beta KW - Colforsin KW - 1F7A44V6OU KW - Phenylephrine KW - 1WS297W6MV KW - 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone KW - 29925-17-5 KW - Cholera Toxin KW - 9012-63-9 KW - Cyclic AMP KW - E0399OZS9N KW - Protein Kinase C KW - EC 2.7.11.13 KW - 3',5'-Cyclic-AMP Phosphodiesterases KW - EC 3.1.4.17 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - 1-Methyl-3-isobutylxanthine KW - TBT296U68M KW - Index Medicus KW - Animals KW - Adrenergic alpha-Agonists -- pharmacology KW - Cholera Toxin -- pharmacology KW - 3',5'-Cyclic-AMP Phosphodiesterases -- antagonists & inhibitors KW - 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone -- pharmacology KW - 3',5'-Cyclic-AMP Phosphodiesterases -- metabolism KW - Rats, Inbred Strains KW - Rats KW - Phorbol Esters -- pharmacology KW - Colforsin -- pharmacology KW - Enzyme Activation -- drug effects KW - Tetradecanoylphorbol Acetate -- pharmacology KW - 1-Methyl-3-isobutylxanthine -- pharmacology KW - Female KW - Phenylephrine -- pharmacology KW - Protein Kinase C -- metabolism KW - Cyclic AMP -- biosynthesis KW - Pineal Gland -- metabolism KW - Receptors, Adrenergic, beta -- physiology KW - Pineal Gland -- drug effects KW - Receptors, Adrenergic, alpha -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78040235?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurochemistry&rft.atitle=Activators+of+protein+kinase+C+act+at+a+postreceptor+site+to+amplify+cyclic+AMP+production+in+rat+pinealocytes.&rft.au=Sugden%2C+D%3BKlein%2C+D+C&rft.aulast=Sugden&rft.aufirst=D&rft.date=1988-01-01&rft.volume=50&rft.issue=1&rft.spage=149&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-26 N1 - Date created - 1988-01-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Response of normal canine ureter to photodynamic therapy using a cylindrical fiber. AN - 78039251; 3336096 AB - Photodynamic therapy (PDT) was performed utilizing a cylindrical optical fiber to determine feasibility of distal ureteral treatment on 10 study and two control NIH foxhounds. The study animals were administered three mg./kg. dihematoporphyrin ether (Photofrin II) intravenously followed 48 hours later by open cystotomy. One ureter was irradiated with 42 joules of 630 nm. light delivered by a 660 micron diameter optical fiber modified for cylindrical light distribution. Intravenous urography was performed both at three days and six weeks post PDT. Hydroureteronephrosis was revealed in one treated ureter and one untreated ureter. Mild dilatation of the ureter was noted by urography in another treated ureter and in one ureter that did not undergo light irradiation; no distal obstruction was revealed in either case by proximal infusion of saline or by histopathology. Nine of the 10 treated ureters were found to have either no abnormal pathology or only minimal lymphocytic infiltration. In this study, the normal ureter was shown to tolerate photodynamic therapy at energy densities equivalent to those used to effect tumor regression and the feasibility of using a cylindrical optical fiber for treatment of ureteral malignancies was confirmed. JF - The Journal of urology AU - Manyak, M J AU - Matthews, D M AU - Smith, P D AU - Nochomovitz, L E AU - Glatstein, E AU - Russo, A AD - Radiation Oncology, Branche National Institutes of Health, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 199 EP - 203 VL - 139 IS - 1 SN - 0022-5347, 0022-5347 KW - Abridged Index Medicus KW - Index Medicus KW - Carcinoma, Transitional Cell -- therapy KW - Animals KW - Hydronephrosis -- etiology KW - Ureteral Diseases -- etiology KW - Humans KW - Urography KW - Dogs KW - Urinary Bladder Neoplasms -- therapy KW - Ureteral Diseases -- diagnostic imaging KW - Models, Biological KW - Male KW - Hydronephrosis -- diagnostic imaging KW - Optical Fibers KW - Ureter -- pathology KW - Photochemotherapy -- adverse effects KW - Fiber Optic Technology -- instrumentation KW - Ureter -- diagnostic imaging KW - Photochemotherapy -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78039251?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+urology&rft.atitle=Response+of+normal+canine+ureter+to+photodynamic+therapy+using+a+cylindrical+fiber.&rft.au=Manyak%2C+M+J%3BMatthews%2C+D+M%3BSmith%2C+P+D%3BNochomovitz%2C+L+E%3BGlatstein%2C+E%3BRusso%2C+A&rft.aulast=Manyak&rft.aufirst=M&rft.date=1988-01-01&rft.volume=139&rft.issue=1&rft.spage=199&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+urology&rft.issn=00225347&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-29 N1 - Date created - 1988-01-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Thrombolysis in Myocardial Infarction (TIMI) Trial--phase I: hemorrhagic manifestations and changes in plasma fibrinogen and the fibrinolytic system in patients treated with recombinant tissue plasminogen activator and streptokinase. AN - 78035289; 3121710 AB - Two hundred ninety patients with acute myocardial infarction were treated according to random assignment with an intravenous infusion of either 80 mg of recombinant tissue plasminogen activator (rt-PA) over 3 h or 1.5 million units of streptokinase over 1 h. Patients received an intravenous bolus of heparin (5,000 U [USP]) before pretreatment coronary angiography and a continuous infusion (1,000 U/h) starting 3 h later. The frequency of major and minor hemorrhagic events (33% rt-PA, 31% streptokinase) and associated transfusions (22% rt-PA, 20% streptokinase) were comparable in both groups. More than 70% of bleeding episodes in each group occurred at catheterization or vascular puncture sites. Precipitable fibrinogen levels, measured in plasma samples collected in the presence of a protease inhibitor (aprotinin), declined in rt-PA and streptokinase groups by averages of 26 and 57% at 3 h and by 33 and 58% at 5 h, respectively (rt-PA versus streptokinase, p less than 0.001). At 5 h the plasma plasminogen declined by 57% (rt-PA) and 82% (streptokinase) (p less than 0.001); plasma fibrin(ogen) degradation products were higher in streptokinase-treated patients (244 +/- 12 micrograms/ml, mean +/- SE) than in rt-PA-treated patients (97 +/- 9 micrograms/ml, p less than 0.001). At 27 h, plasma fibrinogen and plasminogen levels were lower and fibrin(ogen) degradation products higher than pretreatment levels in both groups. The frequency of hemorrhagic events was higher in patients with greater changes in plasma factors at 5 h; within treatment groups the levels of fibrin(ogen) degradation products correlated with bleeding complications (p less than 0.005). Thus, in the doses administered, rt-PA induces systemic fibrinogenolysis that is substantially less intense than that induced by streptokinase. The high frequency of bleeding encountered is related to the protocol used, including vigorous anticoagulation, arterial punctures and thrombolytic therapy. These findings emphasize the need for avoidance of invasive procedures and for meticulous care in the selection and management of patients subjected to thrombolytic therapy. JF - Journal of the American College of Cardiology AU - Rao, A K AU - Pratt, C AU - Berke, A AU - Jaffe, A AU - Ockene, I AU - Schreiber, T L AU - Bell, W R AU - Knatterud, G AU - Robertson, T L AU - Terrin, M L AD - Division of Heart and Vascular Diseases, National Heart, Lung, and Blood Institute, Bethesda, Maryland. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 1 EP - 11 VL - 11 IS - 1 SN - 0735-1097, 0735-1097 KW - Recombinant Proteins KW - 0 KW - Fibrinogen KW - 9001-32-5 KW - Streptokinase KW - EC 3.4.- KW - Tissue Plasminogen Activator KW - EC 3.4.21.68 KW - Abridged Index Medicus KW - Index Medicus KW - Random Allocation KW - Humans KW - Clinical Trials as Topic KW - Thrombocytopenia -- chemically induced KW - Fibrinogen -- analysis KW - Tissue Plasminogen Activator -- therapeutic use KW - Streptokinase -- adverse effects KW - Hemorrhage -- chemically induced KW - Tissue Plasminogen Activator -- adverse effects KW - Streptokinase -- therapeutic use KW - Recombinant Proteins -- adverse effects KW - Fibrinolysis KW - Recombinant Proteins -- therapeutic use KW - Myocardial Infarction -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78035289?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+College+of+Cardiology&rft.atitle=Thrombolysis+in+Myocardial+Infarction+%28TIMI%29+Trial--phase+I%3A+hemorrhagic+manifestations+and+changes+in+plasma+fibrinogen+and+the+fibrinolytic+system+in+patients+treated+with+recombinant+tissue+plasminogen+activator+and+streptokinase.&rft.au=Rao%2C+A+K%3BPratt%2C+C%3BBerke%2C+A%3BJaffe%2C+A%3BOckene%2C+I%3BSchreiber%2C+T+L%3BBell%2C+W+R%3BKnatterud%2C+G%3BRobertson%2C+T+L%3BTerrin%2C+M+L&rft.aulast=Rao&rft.aufirst=A&rft.date=1988-01-01&rft.volume=11&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+College+of+Cardiology&rft.issn=07351097&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-02 N1 - Date created - 1988-02-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Modulation of tissue and epidermal transglutaminases in mouse epidermal cells after treatment with 12-O-tetradecanoylphorbol-13-acetate and/or retinoic acid in vivo and in culture. AN - 78035128; 2891434 AB - Retinoic acid (RA) induces tissue transglutaminase (TGASE) and inhibits terminal differentiation induced either by calcium ion or by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in primary mouse epidermal cells in culture. The relevance of these effects on cultured cells to the antipromoting action of RA was investigated in female BALB/c and CD-1 mice in vivo. Tissue TGASE was distinguished from epidermal TGASE on the basis of different thermolability at pH 9 or elution from the anion exchanger Mono Q. After topical application of 3 to 5 micrograms (10 to 17 nmol) of RA to the shaved back skin, the specific activity of tissue TGASE increased up to 30-fold primarily in the basal cell fraction of Percoll-separated epidermal cells. Enzyme activity returned to basal levels by 7 days. Treatment with TPA (10 micrograms or 17 nmol/mouse) induced an increase in epidermal TGASE which reached a maximum at 12 h after application, primarily in suprabasal cells. RA applied 1 h before TPA caused no reduction of TPA-induced epidermal TGASE, but the increase in tissue TGASE due to RA was markedly inhibited by TPA. The effects of TPA and RA on TGASE activities in primary epidermal cells in culture were similar to those in vivo except that RA reduced the induction of epidermal TGASE by TPA. In culture the induction of epidermal TGASE by TPA was independent of Ca2+ concentration in the medium above 0.03 mM, but cornified envelope formation was markedly enhanced by Ca2+ above the level required for maintaining a basal cell population (0.03 to 0.05 mM). The TPA-induced formation of cornified envelope in the presence of elevated Ca2+ was completely inhibited by RA if cells were pretreated with RA for 24 h. Our results are consistent with RA causing a reprogramming of epidermal cells that alters their response to differentiation stimuli. JF - Cancer research AU - Lichti, U AU - Yuspa, S H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 74 EP - 81 VL - 48 IS - 1 SN - 0008-5472, 0008-5472 KW - Tretinoin KW - 5688UTC01R KW - Transglutaminases KW - EC 2.3.2.13 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Cells, Cultured KW - Enzyme Induction KW - Mice KW - Calcium -- pharmacology KW - Mice, Inbred BALB C KW - Cell Differentiation -- drug effects KW - Time Factors KW - Tretinoin -- pharmacology KW - Skin -- enzymology KW - Skin -- drug effects KW - Transglutaminases -- biosynthesis KW - Tetradecanoylphorbol Acetate -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78035128?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Modulation+of+tissue+and+epidermal+transglutaminases+in+mouse+epidermal+cells+after+treatment+with+12-O-tetradecanoylphorbol-13-acetate+and%2For+retinoic+acid+in+vivo+and+in+culture.&rft.au=Lichti%2C+U%3BYuspa%2C+S+H&rft.aulast=Lichti&rft.aufirst=U&rft.date=1988-01-01&rft.volume=48&rft.issue=1&rft.spage=74&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-08 N1 - Date created - 1988-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Synergistic antiproliferative effects of glucocorticoids and interferon-alpha on some lymphoid cell lines. AN - 78034744; 3335582 AB - The Daudi B lymphoblastoid cell line was previously demonstrated to be highly sensitive to the antiproliferative effect of recombinant interferon-alpha A (rIFN-alpha A). In the present study, glucocorticoid hormones were shown to act synergistically with rIFN-alpha A to further increase the sensitivity of Daudi cells to rIFN-alpha A. At 10(-6) M, dexamethasone, prednisolone, or hydrocortisone alone had little effect on Daudi cell growth, but they greatly potentiated the antiproliferative activity of rIFN-alpha A. The synergy between rIFN-alpha A and glucocorticoids on Daudi cells was not related to the inhibitory effects of glucocorticoids on prostaglandin or leukotriene synthesis, since no synergy was observed between rIFN-alpha A and indomethacin or nordihydroguaiaretic acid. Glucocorticoids and rIFN-alpha A also had appreciable synergistic antiproliferative effects on two out of five other IFN-sensitive lymphoid cell lines. When Raji B lymphoblastoid cells, which were quite resistant to the antiproliferative effect of rIFN-alpha A, were treated with the combination of glucocorticoids and rIFN-alpha A, no significant synergistic effects were observed. The synergistic antiproliferative effects of glucocorticoids and rIFN-alpha A observed with some IFN-sensitive lymphoid cell lines in this in vitro study may have clinical relevance in the treatment of certain lymphoid malignancies that are sensitive to rIFN-alpha A therapy. JF - Journal of cellular physiology AU - Elliott, K R AU - Princler, G L AU - Urba, W J AU - Faltynek, C R AD - Laboratory of Biochemical Physiology, National Cancer Institute, Frederick Cancer Research Facility, Maryland 21701. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 85 EP - 92 VL - 134 IS - 1 SN - 0021-9541, 0021-9541 KW - Glucocorticoids KW - 0 KW - Interferon Type I KW - Recombinant Proteins KW - Masoprocol KW - 7BO8G1BYQU KW - Thymidine KW - VC2W18DGKR KW - Indomethacin KW - XXE1CET956 KW - Index Medicus KW - Thymidine -- metabolism KW - Cell Division -- drug effects KW - Drug Synergism KW - Lymphoma, Non-Hodgkin -- pathology KW - Cell Line KW - Masoprocol -- pharmacology KW - Indomethacin -- pharmacology KW - Lymphoid Tissue -- cytology KW - Interferon Type I -- pharmacology KW - Glucocorticoids -- pharmacology KW - Lymphoid Tissue -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78034744?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cellular+physiology&rft.atitle=Synergistic+antiproliferative+effects+of+glucocorticoids+and+interferon-alpha+on+some+lymphoid+cell+lines.&rft.au=Elliott%2C+K+R%3BPrincler%2C+G+L%3BUrba%2C+W+J%3BFaltynek%2C+C+R&rft.aulast=Elliott&rft.aufirst=K&rft.date=1988-01-01&rft.volume=134&rft.issue=1&rft.spage=85&rft.isbn=&rft.btitle=&rft.title=Journal+of+cellular+physiology&rft.issn=00219541&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Development of murine epidermal cell lines which contain an activated rasHa oncogene and form papillomas in skin grafts on athymic nude mouse hosts. AN - 78034153; 3121168 AB - We have developed four murine epidermal cell lines which form squamous papillomas when grafted to athymic nude mice in a reconstituted skin. Two of the lines, SP-1 and BP-4, were derived from pools of papillomas produced on SENCAR and BALB/c mouse skin, respectively, by initiation with 7,12-dimethylbenz(a)anthracene and promotion with 12-O-tetradecanoylphorbol-13-acetate. Line 308 was derived from BALB/c mouse skin initiated in vivo with 7,12-dimethylbenz(a)anthracene, culture of the epidermal cells, and selection of cells resistant to Ca2+-induced terminal differentiation. Line LC14 was derived from untreated, cultured newborn BALB/c mouse primary epidermal cells which spontaneously developed resistance to Ca2+-induced terminal differentiation. Each line has an activated rasHa gene with a mutation within codon 61. Cells from all four lines, in contrast to normal primary epidermal cells, survive in medium with Ca2+ levels greater than 0.1 mM. Clonal growth studies in culture showed a unique growth pattern for each of the four lines in medium with 1.4 mM and 0.05 mM Ca2+, with or without 12-O-tetradecanoylphorbol-13-acetate. Early passage cells of these lines should provide a valuable resource for detecting genes or genetic alterations which complement an activated ras gene to cause malignant conversion and for studying the biology of tumor promotion. JF - Cancer research AU - Strickland, J E AU - Greenhalgh, D A AU - Koceva-Chyla, A AU - Hennings, H AU - Restrepo, C AU - Balaschak, M AU - Yuspa, S H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 165 EP - 169 VL - 48 IS - 1 SN - 0008-5472, 0008-5472 KW - 9,10-Dimethyl-1,2-benzanthracene KW - 57-97-6 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Neoplasm Transplantation KW - Animals KW - Alleles KW - Transfection KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Mice, Nude KW - Mice KW - Calcium -- pharmacology KW - Mice, Inbred BALB C KW - Cell Line KW - Oncogenes KW - Papilloma -- pathology KW - Papilloma -- etiology KW - Skin Neoplasms -- etiology KW - Skin Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78034153?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Development+of+murine+epidermal+cell+lines+which+contain+an+activated+rasHa+oncogene+and+form+papillomas+in+skin+grafts+on+athymic+nude+mouse+hosts.&rft.au=Strickland%2C+J+E%3BGreenhalgh%2C+D+A%3BKoceva-Chyla%2C+A%3BHennings%2C+H%3BRestrepo%2C+C%3BBalaschak%2C+M%3BYuspa%2C+S+H&rft.aulast=Strickland&rft.aufirst=J&rft.date=1988-01-01&rft.volume=48&rft.issue=1&rft.spage=165&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-08 N1 - Date created - 1988-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Preliminary results of a randomized study of adjuvant radiation therapy in resectable adult retroperitoneal soft tissue sarcomas. AN - 78032912; 3275748 AB - Between January 1980 and September 1985, 35 adult patients with resectable retroperitoneal soft tissue sarcomas were entered on a randomized trial comparing two forms of adjuvant radiation therapy. Fifteen patients received the experimental therapy consisting of intraoperative radiotherapy (IORT) to 20 Gy using high-energy electrons followed by low-dose (35 to 40 Gy) postoperative external beam irradiation. Twenty patients received standard therapy consisting of high-dose (50 to 55 Gy) postoperative external beam irradiation. With a minimum follow-up of 15 months, there is no significant difference in the actuarial disease-free survival (DFS) and overall survival (OS) comparing the two groups (median DFS, 34 months; median OS, 38 months). At 5 years follow-up, approximately 40% of patients are alive and 20% of patients remain disease-free. Although there is a trend towards an improvement in in-field local control in the experimental arm, the predominant pattern of failure in both groups was locoregional within the retroperitoneum and/or peritoneal cavity. Acute and late radiation enteritis were significantly reduced in the experimental group. However, four experimental patients developed late (greater than 6 months following treatment) peripheral neuropathy believed related to the use of IORT; all four recovered. We conclude that there is no difference in the therapeutic effectiveness of the combination of IORT and low-dose external beam radiation compared with conventional high-dose radiation as adjuvant treatment in retroperitoneal sarcomas, although the former appears to be less toxic. Newer combined modality treatment strategies are discussed to improve the prognosis in these patients. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Kinsella, T J AU - Sindelar, W F AU - Lack, E AU - Glatstein, E AU - Rosenberg, S A AD - Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 18 EP - 25 VL - 6 IS - 1 SN - 0732-183X, 0732-183X KW - Index Medicus KW - Postoperative Care KW - Random Allocation KW - Combined Modality Therapy KW - Radiotherapy Dosage KW - Intraoperative Care KW - Humans KW - Adult KW - Clinical Trials as Topic KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Retroperitoneal Neoplasms -- radiotherapy KW - Sarcoma -- radiotherapy KW - Retroperitoneal Neoplasms -- mortality KW - Soft Tissue Neoplasms -- radiotherapy KW - Soft Tissue Neoplasms -- mortality KW - Sarcoma -- mortality KW - Retroperitoneal Neoplasms -- therapy KW - Soft Tissue Neoplasms -- therapy KW - Sarcoma -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78032912?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Preliminary+results+of+a+randomized+study+of+adjuvant+radiation+therapy+in+resectable+adult+retroperitoneal+soft+tissue+sarcomas.&rft.au=Kinsella%2C+T+J%3BSindelar%2C+W+F%3BLack%2C+E%3BGlatstein%2C+E%3BRosenberg%2C+S+A&rft.aulast=Kinsella&rft.aufirst=T&rft.date=1988-01-01&rft.volume=6&rft.issue=1&rft.spage=18&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effect of antibody dose on the imaging and biodistribution of indium-111 9.2.27 anti-melanoma monoclonal antibody. AN - 78032318; 3335926 AB - Eleven patients with metastatic melanoma underwent serial gamma camera imaging and biodistribution measurements after i.v. injection of escalating doses of [111In]9.2.27, an antimelanoma murine monoclonal antibody. Patients received a fixed dose of 1 mg of [111In]9.2.27, with no additional 9.2.27 (five patients), or co-infused with 49 mg (five patients) or 99 mg (one patient) of unlabeled, unconjugated 9.2.27. Higher doses resulted in prolonged blood-pool retention, less uptake in spleen and bone marrow, and appeared to have a positive effect in improving tumor imaging. A dose of 1 mg of 9.2.27 permitted detection of tumors in two of five patients and two of ten lesions, while with greater than or equal to 50 mg, tumors were detected in all patients and in 24 of 32 lesions. Human gamma globulin injected prior to administration of [111In]9.2.27 failed to block the prominent liver, spleen, and bone marrow uptake. No toxicity was observed. These results indicate the feasibility of imaging metastatic melanoma with [111In]9.2.27 and suggest that antibody dose may be a critical determinant of biodistribution and tumor uptake. JF - Journal of nuclear medicine : official publication, Society of Nuclear Medicine AU - Carrasquillo, J A AU - Abrams, P G AU - Schroff, R W AU - Reynolds, J C AU - Woodhouse, C S AU - Morgan, A C AU - Keenan, A M AU - Foon, K A AU - Perentesis, P AU - Marshall, S AD - Nuclear Medicine Department, National Institutes of Health, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 39 EP - 47 VL - 29 IS - 1 SN - 0161-5505, 0161-5505 KW - Antibodies, Monoclonal KW - 0 KW - Antibodies, Neoplasm KW - Indium Radioisotopes KW - Index Medicus KW - Humans KW - Neoplasm Metastasis KW - Middle Aged KW - Tissue Distribution KW - Male KW - Female KW - Radionuclide Imaging KW - Melanoma -- diagnostic imaging KW - Antibodies, Neoplasm -- administration & dosage KW - Melanoma -- immunology KW - Antibodies, Monoclonal -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78032318?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+nuclear+medicine+%3A+official+publication%2C+Society+of+Nuclear+Medicine&rft.atitle=Effect+of+antibody+dose+on+the+imaging+and+biodistribution+of+indium-111+9.2.27+anti-melanoma+monoclonal+antibody.&rft.au=Carrasquillo%2C+J+A%3BAbrams%2C+P+G%3BSchroff%2C+R+W%3BReynolds%2C+J+C%3BWoodhouse%2C+C+S%3BMorgan%2C+A+C%3BKeenan%2C+A+M%3BFoon%2C+K+A%3BPerentesis%2C+P%3BMarshall%2C+S&rft.aulast=Carrasquillo&rft.aufirst=J&rft.date=1988-01-01&rft.volume=29&rft.issue=1&rft.spage=39&rft.isbn=&rft.btitle=&rft.title=Journal+of+nuclear+medicine+%3A+official+publication%2C+Society+of+Nuclear+Medicine&rft.issn=01615505&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-08 N1 - Date created - 1988-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Benzodiazepine enhancement of gamma-aminobutyric acid-mediated chloride ion flux in rat brain synaptoneurosomes. AN - 78029399; 3335847 AB - Benzodiazepine agonists such as Ro 11-6896 [B10(+)], diazepam, clonazepam, and flurazepam were found to enhance muscimol-stimulated 36Cl- uptake into rat cerebral cortical synaptoneurosomes. The rank order of potentiation was B10(+) greater than diazepam greater than clonazepam greater than flurazepam. These benzodiazepines had no effect on 36Cl-uptake in the absence of muscimol. Further, the inactive enantiomer, Ro 11-6893 [B10(-)], and the peripheral benzodiazepine receptor ligand Ro 5-4864 did not potentiate muscimol-stimulated 36Cl- uptake at concentrations up to 10 microM. In contrast, the benzodiazepine receptor inverse agonists ethyl-beta-carboline-3-carboxylate and 6,7-dimethoxy-4-ethyl-beta- carboline-3-carboxylic acid methyl ester inhibited muscimol stimulated 36Cl- uptake. Benzodiazepines and beta-carbolines altered the apparent K0.5 of muscimol-stimulated 36Cl- uptake, without affecting the Vmax. The effects of both benzodiazepine receptor agonists and inverse agonists were reversed by the benzodiazepine antagonists Ro 15-1788 and CGS-8216. These data further confirm that central benzodiazepine receptors modulate the capacity of gamma-aminobutyric acid receptor agonists to enhance chloride transport and provide a biochemical technique for studying benzodiazepine receptor function in vitro. JF - Journal of neurochemistry AU - Morrow, A L AU - Paul, S M AD - Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 302 EP - 306 VL - 50 IS - 1 SN - 0022-3042, 0022-3042 KW - Benzodiazepinones KW - 0 KW - Carbolines KW - Chlorides KW - Benzodiazepines KW - 12794-10-4 KW - methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate KW - 1309288N1J KW - Muscimol KW - 2763-96-4 KW - gamma-Aminobutyric Acid KW - 56-12-2 KW - Clonazepam KW - 5PE9FDE8GB KW - Ro 11-6893 KW - 66921-17-3 KW - beta-carboline-3-carboxylic acid ethyl ester KW - 74214-62-3 KW - Flurazepam KW - IHP475989U KW - Diazepam KW - Q3JTX2Q7TU KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Flurazepam -- pharmacology KW - Animals KW - Diazepam -- pharmacology KW - Carbolines -- pharmacology KW - Drug Synergism KW - Benzodiazepinones -- pharmacology KW - Muscimol -- pharmacology KW - Male KW - Clonazepam -- pharmacology KW - Synaptosomes -- drug effects KW - gamma-Aminobutyric Acid -- pharmacology KW - Brain -- drug effects KW - Chlorides -- metabolism KW - Brain -- metabolism KW - Benzodiazepines -- pharmacology KW - Synaptosomes -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78029399?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurochemistry&rft.atitle=Benzodiazepine+enhancement+of+gamma-aminobutyric+acid-mediated+chloride+ion+flux+in+rat+brain+synaptoneurosomes.&rft.au=Morrow%2C+A+L%3BPaul%2C+S+M&rft.aulast=Morrow&rft.aufirst=A&rft.date=1988-01-01&rft.volume=50&rft.issue=1&rft.spage=302&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-26 N1 - Date created - 1988-01-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Flow cytometric DNA ploidy in colorectal adenomas and family history of colorectal cancer. AN - 78029299; 3334937 AB - Flow cytometric DNA ploidy of colorectal adenomas resected from 34 patients and the corresponding patient family history in first-degree relatives were evaluated. The samples with at least two separate G0-G1 peaks were defined as DNA aneuploid. The correlation between DNA ploidy and family history was evaluated using two-by-two contingency tables. This correlation was highly statistically significant: seven of nine patients (78%) with positive family histories, and five of 25 (20%) with negative family history had adenomas with DNA aneuploid stemlines (P = 0.0068). The overall DNA aneuploidy incidence was 12 in 34 cases (35.2%). The combined information of DNA aneuploidy and positive family history of colorectal cancer in patients with colorectal adenomas may help to better understand the process of colon carcinogenesis and to identify patients who have a higher risk for developing a malignancy. JF - Cancer AU - Sciallero, S AU - Bruno, S AU - Di Vinci, A AU - Geido, E AU - Aste, H AU - Giaretti, W AD - Endoscopic Service, National Cancer Institute (IST), Genoa, Italy. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 114 EP - 120 VL - 61 IS - 1 SN - 0008-543X, 0008-543X KW - DNA, Neoplasm KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Adult KW - Colorectal Neoplasms, Hereditary Nonpolyposis -- analysis KW - Aged KW - Middle Aged KW - Ploidies KW - Male KW - Female KW - Rectal Neoplasms -- genetics KW - Colonic Neoplasms -- genetics KW - Colonic Neoplasms -- analysis KW - Flow Cytometry KW - DNA, Neoplasm -- analysis KW - Rectal Neoplasms -- analysis KW - Adenoma -- genetics KW - Adenoma -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78029299?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer&rft.atitle=Flow+cytometric+DNA+ploidy+in+colorectal+adenomas+and+family+history+of+colorectal+cancer.&rft.au=Sciallero%2C+S%3BBruno%2C+S%3BDi+Vinci%2C+A%3BGeido%2C+E%3BAste%2C+H%3BGiaretti%2C+W&rft.aulast=Sciallero&rft.aufirst=S&rft.date=1988-01-01&rft.volume=61&rft.issue=1&rft.spage=114&rft.isbn=&rft.btitle=&rft.title=Cancer&rft.issn=0008543X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-16 N1 - Date created - 1988-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. AN - 78028741; 3257102 AB - Site-specific mutagenesis was used to introduce amino acid substitutions at the asparagine codons of four conserved potential N-linked glycosylation sites within the gp120 envelope protein of human immunodeficiency virus (HIV). One of these alterations resulted in the production of noninfectious virus particles. The amino acid substitution did not interfere with the synthesis, processing, and stability of the env gene polypeptides gp120 and gp41 or the binding of gp120 to its cellular receptor, the CD4 (T4) molecule. Vaccinia virus recombinants containing wild-type or mutant HIV env genes readily induced syncytia in CD4+ HeLa cells. These results suggest that alterations involving the second conserved domain of the HIV gp120 may interfere with an essential early step in the virus replication cycle other than binding to the CD4 receptor. In long-term cocultures of a T4+ lymphocyte cell line and colon carcinoma cells producing the mutant virus, revertant infectious virions were detected. Molecular characterization of two revertant proviral clones revealed the presence of the original mutation as well as a compensatory amino acid change in another region of HIV gp120. JF - Journal of virology AU - Willey, R L AU - Smith, D H AU - Lasky, L A AU - Theodore, T S AU - Earl, P L AU - Moss, B AU - Capon, D J AU - Martin, M A AD - Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 139 EP - 147 VL - 62 IS - 1 SN - 0022-538X, 0022-538X KW - Antigens, Differentiation, T-Lymphocyte KW - 0 KW - Receptors, Virus KW - Viral Envelope Proteins KW - Index Medicus KW - AIDS/HIV KW - Virus Replication KW - Receptors, Virus -- physiology KW - Cell Fusion KW - T-Lymphocytes -- microbiology KW - DNA Mutational Analysis KW - Antigens, Differentiation, T-Lymphocyte -- physiology KW - Morphogenesis KW - Virion -- physiology KW - Viral Envelope Proteins -- physiology KW - Genes, Viral KW - HIV -- genetics KW - HIV -- pathogenicity KW - Viral Envelope Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78028741?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=In+vitro+mutagenesis+identifies+a+region+within+the+envelope+gene+of+the+human+immunodeficiency+virus+that+is+critical+for+infectivity.&rft.au=Willey%2C+R+L%3BSmith%2C+D+H%3BLasky%2C+L+A%3BTheodore%2C+T+S%3BEarl%2C+P+L%3BMoss%2C+B%3BCapon%2C+D+J%3BMartin%2C+M+A&rft.aulast=Willey&rft.aufirst=R&rft.date=1988-01-01&rft.volume=62&rft.issue=1&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-21 N1 - Date created - 1988-01-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Mol Biol. 1967 Jun 14;26(2):365-9 [4291934] J Virol. 1987 Aug;61(8):2639-41 [2439707] J Virol. 1973 Dec;12(6):1457-65 [4357516] Virology. 1974 Feb;57(2):475-90 [4361457] Science. 1977 Apr 8;196(4286):175-7 [322278] Science. 1977 Apr 8;196(4286):180-2 [322279] Cell. 1987 Sep 11;50(6):975-85 [2441877] Rev Infect Dis. 1980 Jan-Feb;2(1):40-61 [6994202] J Virol. 1981 Apr;38(1):239-48 [6165830] Science. 1983 May 20;220(4599):868-71 [6189183] J Virol. 1984 Mar;49(3):857-64 [6321770] Virology. 1984 Feb;133(1):65-76 [6199893] Gene. 1977 May;1(3-4):255-80 [338419] Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 [271968] Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 [286319] Science. 1984 May 4;224(4648):500-3 [6200936] Science. 1984 Aug 24;225(4664):840-2 [6206563] DNA. 1984 Aug;3(4):339-43 [6489095] Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5 [6438633] DNA. 1984 Dec;3(6):479-88 [6096101] Science. 1985 Feb 1;227(4686):484-92 [2578227] Cell. 1985 Jan;40(1):9-17 [2981635] Proc Natl Acad Sci U S A. 1985 Jul;82(13):4539-43 [2989831] J Immunol. 1985 Nov;135(5):3151-62 [2995487] Annu Rev Immunol. 1985;3:477-500 [2415141] Science. 1986 Jan 24;231(4736):382-5 [3001934] Anal Biochem. 1986 Apr;154(1):353-60 [3010776] Cell. 1986 Jun 6;45(5):637-48 [2423250] Cell. 1986 Jul 4;46(1):63-74 [2424612] Proc Natl Acad Sci U S A. 1986 Jul;83(14):5038-42 [3014529] J Virol. 1986 Aug;59(2):284-91 [3016298] Nature. 1986 Jul 31-Aug 6;322(6078):470-4 [3016552] Mol Cell Biol. 1985 Dec;5(12):3403-9 [3939316] J Immunol. 1986 Nov 1;137(9):2937-44 [2428879] Cell. 1986 Nov 7;47(3):333-48 [3094962] Proc Natl Acad Sci U S A. 1986 Nov;83(21):8380-4 [3490666] J Virol. 1987 Jan;61(1):209-13 [3640832] Cell. 1987 Feb 27;48(4):691-701 [3643816] Cell. 1987 Jun 5;49(5):659-68 [3107838] Nature. 1970 Aug 15;227(5259):680-5 [5432063] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Combined effects of chemotherapy and interleukin 2 in the therapy of mice with advanced pulmonary tumors. AN - 78026287; 3257159 AB - We have evaluated the effects of chemotherapeutic agents on the toxicity and antitumor benefit of therapy of established murine tumors by high-dose interleukin 2 (IL-2). Cyclophosphamide (Cy), doxorubicin, and bischloroethylnitrosourea were given to normal mice prior to IL-2 administration to test the effects of these agents on IL-2-induced toxicity. Cy at doses of 100 mg/kg and 150 mg/kg completely protected mice from a 100% lethal dose of IL-2, and doses of 50 mg/kg and 150 mg/kg allowed the administration of a median of 4.5 and 10.0 more doses of IL-2, respectively, before death from IL-2 toxicity occurred. Doxorubicin at 8 mg/kg and bischloroethylnitrosourea at 20 mg/kg did not impact on toxicity in IL-2-treated mice. In mice bearing pulmonary metastases of the weakly immunogenic MCA-105 sarcoma, IL-2 increased median survival time from 33 (no IL-2) to greater than 60 days for all doses of IL-2 tested when combined with a single injection of Cy at 75 mg/kg (P less than 0.002). Increasing doses of either Cy or IL-2 produced increasing benefits on survival which were always greater than either treatment alone. These effects of Cy and IL-2 were also seen in mice bearing the nonimmunogenic MCA-101 sarcoma and a murine adenocarcinoma (MCA-38). Doxorubicin and bischloroethylnitrosourea did not consistently enhance the effects of IL-2 treatment. Cy appears to reduce the yield of in vivo generated lymphokine-activated killer cells, but these lymphokine-activated killer cells are still lytic for fresh tumor targets in vitro. Thus, the mechanism of this synergy does not appear to involve stimulation of lymphokine-activated killer cell activity, but may in part involve reduction of tumor burden by the chemotherapeutic agent, an increase in susceptibility of tumor to cellular immune lysis, and/or a decrease in suppressor cell activity mediated by the chemotherapy. JF - Cancer research AU - Papa, M Z AU - Yang, J C AU - Vetto, J T AU - Shiloni, E AU - Eisenthal, A AU - Rosenberg, S A AD - Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01/01/ PY - 1988 DA - 1988 Jan 01 SP - 122 EP - 129 VL - 48 IS - 1 SN - 0008-5472, 0008-5472 KW - Interleukin-2 KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Animals KW - Lymphocytes -- immunology KW - Dose-Response Relationship, Drug KW - Mice, Inbred C57BL KW - Mice KW - Killer Cells, Natural -- immunology KW - Killer Cells, Natural -- drug effects KW - Interleukin-2 -- administration & dosage KW - Lung Neoplasms -- drug therapy KW - Interleukin-2 -- toxicity KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78026287?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Combined+effects+of+chemotherapy+and+interleukin+2+in+the+therapy+of+mice+with+advanced+pulmonary+tumors.&rft.au=Papa%2C+M+Z%3BYang%2C+J+C%3BVetto%2C+J+T%3BShiloni%2C+E%3BEisenthal%2C+A%3BRosenberg%2C+S+A&rft.aulast=Papa&rft.aufirst=M&rft.date=1988-01-01&rft.volume=48&rft.issue=1&rft.spage=122&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-08 N1 - Date created - 1988-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Tamoxifen inhibits estrogen-induced hepatic injury in hamsters. AN - 78024597; 3335202 AB - Estrogens have an unusual toxic effect on the liver of two hamster species, the Armenian and the Chinese hamster. The hepatotoxicity was detectable clinically by hyperbilirubinemia and confirmed histologically by the presence of hepatic degenerative-regenerative changes. Administration of tamoxifen with estrogen [either ethynyl estradiol or diethylstilbestrol (DES)] completely abrogated the hepatotoxic effects, suggesting that estrogen receptor (ER) was necessary for estrogen to damage liver. In Armenian hamsters, estrogens decreased hepatic synthesis of female protein (FP); tamoxifen also abolished this DES effect and resulted in a net increase in serum FP levels. DES administration produced higher serum bilirubin levels and lower serum FP levels in females than in males. Paradoxically, tamoxifen blocked these DES effects more effectively and efficiently in females than in males. Estrogens did not injure uteri of Armenian and Chinese hamsters and were nontoxic to livers of other hamsters species, such as Syrian and Turkish. This model provides another perspective of the acute cellular derangement that can be effected by estrogen-ER complex and may indicate a yet unknown mode of ER action. JF - Endocrinology AU - Coe, J E AU - Ross, M J AD - Department of Health and Human Services, National Institutes of Health, Hamilton, Montana 59840. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 137 EP - 144 VL - 122 IS - 1 SN - 0013-7227, 0013-7227 KW - Tamoxifen KW - 094ZI81Y45 KW - Diethylstilbestrol KW - 731DCA35BT KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Sex Factors KW - Cricetulus KW - Time Factors KW - Species Specificity KW - Male KW - Female KW - Cricetinae KW - Tamoxifen -- pharmacology KW - Liver -- pathology KW - Liver -- drug effects KW - Diethylstilbestrol -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78024597?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Endocrinology&rft.atitle=Tamoxifen+inhibits+estrogen-induced+hepatic+injury+in+hamsters.&rft.au=Coe%2C+J+E%3BRoss%2C+M+J&rft.aulast=Coe&rft.aufirst=J&rft.date=1988-01-01&rft.volume=122&rft.issue=1&rft.spage=137&rft.isbn=&rft.btitle=&rft.title=Endocrinology&rft.issn=00137227&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-03 N1 - Date created - 1988-02-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Site-selective cAMP analogs at micromolar concentrations induce growth arrest and differentiation of acute promyelocytic, chronic myelocytic, and acute lymphocytic human leukemia cell lines. AN - 78024267; 2825845 AB - Cyclic AMP (cAMP)-dependent protein kinase may play a role in the functional and morphological differentiation of leukemic cells. In this study, we showed that the cAMP analogs, potent activators of protein kinase recently shown to be selective for either site 1 or site 2 cAMP binding sites of protein kinase, demonstrate potent growth inhibition of acute promyelocytic, chronic myelocytic, and acute lymphocytic leukemic cell lines with no sign of toxicity. The growth inhibition accompanied monocytic differentiation in HL-60 cells and a loss of nuclear terminal deoxynucleotidyl transferase activity in Molt-4 leukemic cells. The growth inhibition also paralleled a decrease in c-myc protein and RI cAMP receptor protein. Thus, cAMP analogs selective for either site 1 or site 2 of the protein kinase appear to restore a coupling of proliferation and maturation in leukemic cells. JF - Blood AU - Tortora, G AU - Tagliaferri, P AU - Clair, T AU - Colamonici, O AU - Neckers, L M AU - Robins, R K AU - Cho-Chung, Y S AD - Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 230 EP - 233 VL - 71 IS - 1 SN - 0006-4971, 0006-4971 KW - Neoplasm Proteins KW - 0 KW - Cyclic AMP KW - E0399OZS9N KW - Protein Kinases KW - EC 2.7.- KW - Abridged Index Medicus KW - Index Medicus KW - Protein Kinases -- metabolism KW - Humans KW - Enzyme Activation -- drug effects KW - Cell Division -- drug effects KW - Cell Differentiation -- drug effects KW - Neoplasm Proteins -- metabolism KW - Tumor Cells, Cultured -- drug effects KW - Cyclic AMP -- pharmacology KW - Leukemia, Lymphoid -- pathology KW - Cyclic AMP -- analogs & derivatives KW - Leukemia, Myeloid, Acute -- pathology KW - Leukemia, Myeloid -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78024267?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Site-selective+cAMP+analogs+at+micromolar+concentrations+induce+growth+arrest+and+differentiation+of+acute+promyelocytic%2C+chronic+myelocytic%2C+and+acute+lymphocytic+human+leukemia+cell+lines.&rft.au=Tortora%2C+G%3BTagliaferri%2C+P%3BClair%2C+T%3BColamonici%2C+O%3BNeckers%2C+L+M%3BRobins%2C+R+K%3BCho-Chung%2C+Y+S&rft.aulast=Tortora&rft.aufirst=G&rft.date=1988-01-01&rft.volume=71&rft.issue=1&rft.spage=230&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-20 N1 - Date created - 1988-02-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Experimental chemotherapy-induced skin necrosis in swine. Mechanistic studies of anthracycline antibiotic toxicity and protection with a radical dimer compound. AN - 78019913; 3121673 AB - The reactivity of antitumor anthracycline and mitomycin C antibiotics with the oxomorpholinyl radical dimers, bi(3,5,5-trimethyl-2-oxomorpholin-3-yl) (TM3) and bi(3,5-dimethyl-5-hydroxymethyl-2-oxomorpholin-3-yl) (DHM3), was studied in vitro. The oxomorpholinyl radical reduced daunorubicin to a quinone methide intermediate that reacted with solvent to form 7-deoxydaunorubicinone. The solvolysis reaction followed first order kinetics, and the reactivity rate constants (k2) measured for seven anthracycline analogues ranged from 2 X 10(-2) s-1 to 8.0 X 10(-4) s-1. The chemical reactivity of each anthracycline quinone methide correlated with the total skin toxicity caused by the respective parent anthracycline following injection into swine skin. Microscopic examination of experimental lesions in swine skin resemble those observed in humans after inadvertant chemotherapy extravasation. Hydrocortisone sodium succinate was not effective for the treatment of doxorubicin-induced skin necrosis, whereas DHM3 was effective for the treatment of skin necrosis caused by all seven anthracyclines and by the quinone containing antibiotic, mitomycin C. JF - The Journal of clinical investigation AU - Averbuch, S D AU - Boldt, M AU - Gaudiano, G AU - Stern, J B AU - Koch, T H AU - Bachur, N R AD - Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 142 EP - 148 VL - 81 IS - 1 SN - 0021-9738, 0021-9738 KW - Antibiotics, Antineoplastic KW - 0 KW - Free Radicals KW - Mitomycins KW - Morpholines KW - Mitomycin KW - 50SG953SK6 KW - Doxorubicin KW - 80168379AG KW - bis(3,5-dimethyl-5-hydroxymethyl-2-oxomorpholin-3-yl) KW - 99634-12-5 KW - Abridged Index Medicus KW - Index Medicus KW - Swine KW - Animals KW - Necrosis KW - Drug Interactions KW - Doxorubicin -- toxicity KW - Mitomycins -- pharmacology KW - Swine, Miniature KW - Female KW - Skin Diseases -- drug therapy KW - Morpholines -- therapeutic use KW - Morpholines -- pharmacology KW - Skin Diseases -- pathology KW - Skin Diseases -- chemically induced KW - Antibiotics, Antineoplastic -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78019913?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+clinical+investigation&rft.atitle=Experimental+chemotherapy-induced+skin+necrosis+in+swine.+Mechanistic+studies+of+anthracycline+antibiotic+toxicity+and+protection+with+a+radical+dimer+compound.&rft.au=Averbuch%2C+S+D%3BBoldt%2C+M%3BGaudiano%2C+G%3BStern%2C+J+B%3BKoch%2C+T+H%3BBachur%2C+N+R&rft.aulast=Averbuch&rft.aufirst=S&rft.date=1988-01-01&rft.volume=81&rft.issue=1&rft.spage=142&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+clinical+investigation&rft.issn=00219738&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-09 N1 - Date created - 1988-02-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer. 1971 Oct;28(4):837-43 [4329505] J Invest Dermatol. 1972 Mar;58(3):114-23 [4622425] Cancer. 1976 Sep;38(3):1087-94 [953958] Transplantation. 1976 Dec;22(6):559-67 [137560] Science. 1977 Aug 5;197(4303):527-32 [877572] Am J Nurs. 1979 Jan;79(1):94-6 [252885] Inflammation. 1980 Jun;4(2):233-47 [6446523] Cancer Treat Rev. 1980 Mar;7(1):17-27 [7397706] Cancer Chemother Pharmacol. 1980;5(1):17-20 [7460191] J Biol Chem. 1981 May 25;256(10):4747-56 [6262301] Cancer Res. 1982 Mar;42(3):1078-81 [6277472] Biochem Pharmacol. 1982 Feb 15;31(4):575-81 [6279110] Cancer. 1982 May 1;49(9):1796-9 [7042076] Cancer Res. 1982 Sep;42(9):3574-82 [6809311] Cancer. 1983 Mar 15;51(6):1080-2 [6401597] J Hand Surg Am. 1983 Jan;8(1):32-8 [6827049] Cancer Chemother Pharmacol. 1983;11(2):91-3 [6627601] Cancer Treat Rep. 1984 Jul-Aug;68(7-8):939-45 [6378380] Plast Reconstr Surg. 1985 Mar;75(3):397-405 [3883378] Biochemistry. 1985 Jul 2;24(14):3562-71 [3862429] Cancer Res. 1985 Dec;45(12 Pt 1):6200-4 [4063971] J Clin Oncol. 1986 Jan;4(1):88-94 [3941333] Environ Health Perspect. 1985 Dec;64:4-18 [3913602] Cancer Treat Rep. 1986 Apr;70(4):503-7 [3009011] J Hand Surg Am. 1986 May;11(3):388-96 [3711613] Mol Pharmacol. 1986 Jun;29(6):622-8 [3086708] J Invest Dermatol. 1964 Jul;42:11-21 [14209446] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Ram seminal vesicle microsome-catalyzed activation of benzidine and related compounds: dissociation of mutagenesis from peroxidase-catalyzed formation of DNA-reactive material. AN - 78019898; 3335047 AB - Ram seminal vesicle (RSV) microsomal preparations activate benzidine and other arylamines to mutagenic species in a modified Ames assay. We have examined the mechanism of this activation process in more detail. The mutagenic effect was neither arachidonic acid-dependent nor indomethacin inhibitable. The mutagenic species was stable for at least 30 min in experiments in which addition of bacteria was delayed. Acetylbenzidine was a much more potent mutagen than benzidine in this system. Substitution of the acetylase-deficient tester strain TA98/1,8-DNP6 for strain TA98 markedly reduced the mutagenicity of acetylbenzidine and completely eliminated the mutagenicity of benzidine. Benzidine analogues 3,3'-dimethoxybenzidine (o-dianisidine), o-tolidine and 3,3',-5,5'-tetramethylbenzidine were not mutagenic in the RSV activation system. RSV-dependent activation of all radiolabeled congeners examined resulted in covalent binding to calfthymus DNA. The rank order of binding was: 3,3'-dichlorobenzidine greater than benzidine greater than o-dianisidine greater than acetylbenzidine greater than tetramethylbenzidine. This binding required active enzyme and arachidonic acid or hydrogen peroxide. The reactive species was short-lived: delayed addition of DNA reduced the level of binding nearly to zero. Binding was inhibitable by indomethacin, but this inhibition was incomplete in the cases of dichlorobenzidine and acetylbenizidine. We conclude that the extracellular generation of peroxidase-catalyzed oxidation products does not explain the RSV microsome-dependent mutagenicity observed with these compounds. JF - Carcinogenesis AU - Petry, T W AU - Eling, T E AU - Chiu, A L AU - Josephy, P D AD - Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1988/01// PY - 1988 DA - January 1988 SP - 51 EP - 57 VL - 9 IS - 1 SN - 0143-3334, 0143-3334 KW - Benzidines KW - 0 KW - Phenols KW - Phenol KW - 339NCG44TV KW - DNA KW - 9007-49-2 KW - Peroxidases KW - EC 1.11.1.- KW - Glutathione KW - GAN16C9B8O KW - Ascorbic Acid KW - PQ6CK8PD0R KW - Indomethacin KW - XXE1CET956 KW - Index Medicus KW - Animals KW - Mutagenicity Tests KW - Biotransformation KW - Sheep KW - Phenols -- metabolism KW - Ascorbic Acid -- pharmacology KW - Mutation KW - Glutathione -- pharmacology KW - Male KW - Peroxidases -- metabolism KW - Indomethacin -- pharmacology KW - Benzidines -- metabolism KW - Seminal Vesicles -- metabolism KW - Microsomes -- metabolism KW - DNA -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78019898?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Ram+seminal+vesicle+microsome-catalyzed+activation+of+benzidine+and+related+compounds%3A+dissociation+of+mutagenesis+from+peroxidase-catalyzed+formation+of+DNA-reactive+material.&rft.au=Petry%2C+T+W%3BEling%2C+T+E%3BChiu%2C+A+L%3BJosephy%2C+P+D&rft.aulast=Petry&rft.aufirst=T&rft.date=1988-01-01&rft.volume=9&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-18 N1 - Date created - 1988-02-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - 4-chloro-3-hydroxyanthranilate inhibits brain 3-hydroxyanthranate oxidase. AN - 733575790; 19651098 AB - Quinolinic acid is synthesized from 3-hydroxyanthranilic acid via 3-hydroxyanthranilic acid oxidase. In liver, 4-chloro-3-hydroxyanthranilic acid inhibits 3-hydroxyanthranilic acid oxidase. To determine whether 4-chloro-3-hydroxyanthranilic acid also inhibits 3-hydroxyanthranilic acid oxidase in brain, 3-hydroxyanthranilic acid was injected into the cisterna magna of rats either with or without 4-chloro-3 hydroxyanthranilic acid. 3-Hydroxyanthranilic acid increased quinolinic acid concentrations throughout the brain. 4-Chloro-3-hydroxyanthranilic acid attenuated increases in brain quinolinic acid. These observations indicate that 4-chloro-3-hydroxyanthranilic acid inhibits 3-hydroxyanthranilic acid oxidase in brain. Quinolinic acid is a well established systemic metabolite of l-tryptophan which has been shown to be present in brain (Wolfensberger et al., 1983; Heyes and Markey, 1988a). QUIN has proved to be a convulsant (Lapin, 1982), neurotoxin (Schwarcz et al., 1983) and agonist of N-methyl-D-aspartate receptors (Perkins and Stone, 1983) when injected directly into the central nervous system of experimental animals. Therefore increased concentrations of QUIN in brain may have neoropathologic consequences. l-Tryptophan is converted to QUIN via the kynurenine pathway. The precursor of QUIN, 2-amino-3-carboxymuconic semialdehyde is synthesized from 3-hydroxyanthranilic acid (3-HAA) by the action of 3-hydroxyanthranilic acid oxidase (3-HAA/OX) in liver and brain (Foster et al., 1986; Okuno et al., 1987). QUIN is then formed from 2-amino-3-carboxymuconic semialdehyde by a spontaneous, non-enzymatic reaction. In liver, 3-HAA/OX is inhibited by 4-chloro-3-hydroxyantranilic acid (CL-HAA; Parli et al., 1980). In the present study, rats were given an intracisternal injection of 3-HAA and the resultant increases in regional brain QUIN concentrations quantified by gas chromatography/mass spectrometry (Heyes and Markey, 1988a,b). To determine whether CL-HAA inhibit 3-hydroxyanthranilic acid oxidase in brain, CL-HAA was co-administered with 3-HAA to see whether increases in QUIN were attenuated. JF - Neurochemistry international AU - Heyes, M P AU - Hutto, B AU - Markey, S P AD - Laboratory of Neurophysiology, Building 10, Room 3D40 National Institute of Mental Health, Bethesda, MD 20892 USA. Y1 - 1988 PY - 1988 DA - 1988 SP - 405 EP - 408 VL - 13 IS - 3 SN - 0197-0186, 0197-0186 UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/733575790?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurochemistry+international&rft.atitle=4-chloro-3-hydroxyanthranilate+inhibits+brain+3-hydroxyanthranate+oxidase.&rft.au=Heyes%2C+M+P%3BHutto%2C+B%3BMarkey%2C+S+P&rft.aulast=Heyes&rft.aufirst=M&rft.date=1988-01-01&rft.volume=13&rft.issue=3&rft.spage=405&rft.isbn=&rft.btitle=&rft.title=Neurochemistry+international&rft.issn=01970186&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2009-12-14 N1 - Date created - 2009-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The Structural Pattern Theory of Modern Society AN - 61019687; 90V1785 AB - A general trend in modern society is identified as the transformation of a strategy of unitary economic growth into a strategy of overall social development that includes attention to the relations among society, economy, & scientific technology. The historical materialist perspective of Karl Marx has reached a new stage in history, because, unlike in Marx's time, it need not emphasize criticism of the idealist conception of history. A summary of the structural pattern theory of modern society is offered in which society is seen -- as Marx conceived it -- as a unity of humans & nature. Three main stratifications form its overall structure: the ecological system between humans & nature; the major subsystems of economy, science, culture, etc; & the nucleus of productive force, the individual. 2 Figures. Modified AA JF - Revue Internationale de Sociologie/International Review of Sociology (nouvelle serie/new series) AU - Shuwei, Song AD - Chinese Academy Social Sciences, 5 Jianguomen Nei Da Jie 5 Hao Beijing Y1 - 1988///0, PY - 1988 DA - 0, 1988 SP - 7 EP - 23 IS - 3 SN - 0390-6701, 0390-6701 KW - modern society, structural pattern theory KW - Marxist Sociology KW - Social Development KW - Social Structure KW - Modern Society KW - article KW - 0715: social change and economic development; social change & economic development UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/61019687?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Asocabs&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Revue+Internationale+de+Sociologie%2FInternational+Review+of+Sociology+%28nouvelle+serie%2Fnew+series%29&rft.atitle=The+Structural+Pattern+Theory+of+Modern+Society&rft.au=Shuwei%2C+Song&rft.aulast=Shuwei&rft.aufirst=Song&rft.date=1988-01-01&rft.volume=&rft.issue=3&rft.spage=7&rft.isbn=&rft.btitle=&rft.title=Revue+Internationale+de+Sociologie%2FInternational+Review+of+Sociology+%28nouvelle+serie%2Fnew+series%29&rft.issn=03906701&rft_id=info:doi/ LA - English DB - Sociological Abstracts N1 - Date revised - 2007-04-01 N1 - Last updated - 2016-09-28 N1 - CODEN - RISOD6 N1 - SubjectsTermNotLitGenreText - Modern Society; Social Development; Marxist Sociology; Social Structure ER - TY - JOUR T1 - A report of geological observations on northern segment (Baotou to Mondula) of the geoscience transect from Xiangshui, Jiangsu, to Mandula, Nei Monggol AN - 50780771; 1989-062032 JF - Dizhen Dizhi = Seismology and Geology AU - Ma, Xingyuan AU - Wang, Ji AU - Li, Shuangqing AU - Liu, De-jian AU - Bai, Yunhong AU - Zhou, Chun-ping AU - Huang, Guo-hua Y1 - 1988 PY - 1988 DA - 1988 SP - 45 EP - 50 PB - Dizhen Chubanshe, Beijing VL - 10 IS - 4 SN - 0253-4967, 0253-4967 KW - Inner Mongolia China KW - Far East KW - upper Precambrian KW - Precambrian KW - Mandala KW - Chartai Aulacogen KW - Paleozoic KW - Northern China KW - Jiangsu China KW - Variscan Orogeny KW - basement KW - aulacogens KW - Proterozoic KW - Bayan Obo Geocline KW - Mesoproterozoic KW - Caledonian Orogeny KW - structural geology KW - folds KW - tectonics KW - Asia KW - China KW - 16:Structural geology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/50780771?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ageorefmodule&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Dizhen+Dizhi+%3D+Seismology+and+Geology&rft.atitle=A+report+of+geological+observations+on+northern+segment+%28Baotou+to+Mondula%29+of+the+geoscience+transect+from+Xiangshui%2C+Jiangsu%2C+to+Mandula%2C+Nei+Monggol&rft.au=Ma%2C+Xingyuan%3BWang%2C+Ji%3BLi%2C+Shuangqing%3BLiu%2C+De-jian%3BBai%2C+Yunhong%3BZhou%2C+Chun-ping%3BHuang%2C+Guo-hua&rft.aulast=Ma&rft.aufirst=Xingyuan&rft.date=1988-01-01&rft.volume=10&rft.issue=4&rft.spage=45&rft.isbn=&rft.btitle=&rft.title=Dizhen+Dizhi+%3D+Seismology+and+Geology&rft.issn=02534967&rft_id=info:doi/ LA - Chinese DB - GeoRef N1 - Copyright - GeoRef, Copyright 2014, American Geosciences Institute. N1 - Date revised - 1989-01-01 N1 - Number of references - 5 N1 - Document feature - illus. incl. sects., geol. sketch map N1 - Last updated - 2014-03-14 N1 - SubjectsTermNotLitGenreText - Asia; aulacogens; basement; Bayan Obo Geocline; Caledonian Orogeny; Chartai Aulacogen; China; Far East; folds; Inner Mongolia China; Jiangsu China; Mandala; Mesoproterozoic; Northern China; Paleozoic; Precambrian; Proterozoic; structural geology; tectonics; upper Precambrian; Variscan Orogeny ER - TY - JOUR T1 - The Silurian biostratigraphy of Inner Mongolia AN - 50682048; 1990-042401 JF - Diceng Gushengwu Lunwen Ji = Professional Papers of Stratigraphy and Palaeontology AU - Li, Wenguo Y1 - 1988 PY - 1988 DA - 1988 SP - 180 EP - 192 PB - Ti Chih Chu Pan She, Beijing VL - 21 SN - 0254-1351, 0254-1351 KW - stratigraphy KW - Inner Mongolia China KW - Far East KW - biostratigraphy KW - Barendel Formation KW - Paleozoic KW - Silurian KW - marine environment KW - Brachiopoda KW - Invertebrata KW - Graptolithina KW - Asia KW - China KW - 12:Stratigraphy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/50682048?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ageorefmodule&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diceng+Gushengwu+Lunwen+Ji+%3D+Professional+Papers+of+Stratigraphy+and+Palaeontology&rft.atitle=The+Silurian+biostratigraphy+of+Inner+Mongolia&rft.au=Li%2C+Wenguo&rft.aulast=Li&rft.aufirst=Wenguo&rft.date=1988-01-01&rft.volume=21&rft.issue=&rft.spage=180&rft.isbn=&rft.btitle=&rft.title=Diceng+Gushengwu+Lunwen+Ji+%3D+Professional+Papers+of+Stratigraphy+and+Palaeontology&rft.issn=02541351&rft_id=info:doi/ LA - Chinese DB - GeoRef N1 - Copyright - GeoRef, Copyright 2012, American Geosciences Institute. N1 - Date revised - 1990-01-01 N1 - Number of references - 9 N1 - Document feature - illus. incl. 4 tables N1 - Last updated - 2012-06-07 N1 - SubjectsTermNotLitGenreText - Asia; Barendel Formation; biostratigraphy; Brachiopoda; China; Far East; Graptolithina; Inner Mongolia China; Invertebrata; marine environment; Paleozoic; Silurian; stratigraphy ER - TY - JOUR T1 - Amorphous Soluble Cyclodextrins: Pharmaceutical and Therapeutic Uses AN - 21203560; 11612576 AB - Amorphous water soluble derivatives of cyclodextrins are potent, nontoxic solubilizers of drugs and lipids. Their use as excipients which enable effective oral administration of sex hormones is described. Furthermore, these com pounds were used intravenously as an active drug to assist an organism in unloading a toxic lipophile. JF - Journal of Bioactive and Compatible Polymers AU - Pitha, Josef AD - National Institutes of Health, National Institute on Aging Gerontology Research Center, Macromolecular Chemistry Section Baltimore, MD 21224, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 157 EP - 163 PB - Sage Publications Ltd., 6 Bonhill St. London EC2A 4PU UK VL - 3 IS - 2 SN - 0883-9115, 0883-9115 KW - Biotechnology and Bioengineering Abstracts KW - Unloading KW - cyclodextrin KW - Lipids KW - Oral administration KW - Pharmaceuticals KW - Drugs KW - Sex hormones KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21203560?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bioactive+and+Compatible+Polymers&rft.atitle=Amorphous+Soluble+Cyclodextrins%3A+Pharmaceutical+and+Therapeutic+Uses&rft.au=Pitha%2C+Josef&rft.aulast=Pitha&rft.aufirst=Josef&rft.date=1988-01-01&rft.volume=3&rft.issue=2&rft.spage=157&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bioactive+and+Compatible+Polymers&rft.issn=08839115&rft_id=info:doi/10.1177%2F088391158800300206 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-01-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Unloading; cyclodextrin; Lipids; Oral administration; Pharmaceuticals; Drugs; Sex hormones DO - http://dx.doi.org/10.1177/088391158800300206 ER - TY - JOUR T1 - Carcinogenesis by nitroso-2-hydroxyethylurea in splenectomized hamsters. AN - 15473057; 2200481 AB - Several nitrosoalkylureas tested for carcinogenic activity in Syrian hamsters have as their main effect the induction of hemangiosarcomas of the spleen, many of which appear to metastasize to the liver. To investigate whether any of these lesions in the liver might not be metastases, a group of female Syrian hamsters was surgically splenectomized and treated with nitroso-2-hydroxythylurea (NHEU) dissolved in corn oil/ethyl acetate once a week for 22 weeks. The animals survived much longer (median 45 weeks) than a comparable group of unsplenectomized female hamsters given identical treatment (median 24 weeks). The absence of hemangiosarcomas of the liver in the splenectomized hamsters showed that the presumed metastases seen in intact animals were, in fact, just that. The compound did not produce a concomitant increase in tumors of other types. JF - Cancer Letters AU - Lijinsky, W AU - Kovatch, R M AU - Thomas, B J AD - NCI-Frederick Cancer Res. Facil., BRI-Basic Res. Program, Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 199 EP - 202 VL - 41 IS - 2 SN - 0304-3835, 0304-3835 KW - nitroso-2-hydroxyethylurea KW - hamsters KW - Toxicology Abstracts KW - carcinogenesis KW - X 24200:Nitrosamines & related compounds UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15473057?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Letters&rft.atitle=Carcinogenesis+by+nitroso-2-hydroxyethylurea+in+splenectomized+hamsters.&rft.au=Lijinsky%2C+W%3BKovatch%2C+R+M%3BThomas%2C+B+J&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=41&rft.issue=2&rft.spage=199&rft.isbn=&rft.btitle=&rft.title=Cancer+Letters&rft.issn=03043835&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - carcinogenesis ER - TY - JOUR T1 - Carcinogenesis by nitrosobis-(2-oxopropyl)amine labeled with deuterium and by nitroso-2-hydroxypropyl-2-oxopropylamine in rats and hamsters. AN - 15444832; 2179259 AB - The effects of the labeling with deuterium of the alpha -methylene groups of the carcinogen nitrosobis-(2-oxopropyl)amine (NBOP) on its carcinogenic effectiveness in rats and hamsters have been studied. The greater strength of the C-D bond compared with the C-H bond often leads to slower metabolism and lesser carcinogenic activity. When NBOP and NBOP-d sub(4) were given to male and female rats in drinking water at equimolar doses, the mortality rate from tumors was lower in the rats given the deuterium-labeled compound, although the results were statistically significant only in males. The incidences of tumors of several groups was similar for NBOP and NBOP-d sub(4), but there was a marked difference between males and females, females having a high incidence of liver tumors, and males very few. JF - Cancer Letters AU - Lijinsky, W AU - Saavedra, JE AU - Kovatch, R M AD - NCI-Frederick Cancer Res. Fac., BRI-Basic Res. Program, Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 37 EP - 41 VL - 42 IS - 1-2 SN - 0304-3835, 0304-3835 KW - N-nitrosobis(2-oxopropyl)amine KW - nitroso-2-hydroxypropyl-2-oxopropylamine KW - rats KW - hamsters KW - deuterium KW - Toxicology Abstracts KW - carcinogenesis KW - X 24200:Nitrosamines & related compounds UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15444832?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Letters&rft.atitle=Carcinogenesis+by+nitrosobis-%282-oxopropyl%29amine+labeled+with+deuterium+and+by+nitroso-2-hydroxypropyl-2-oxopropylamine+in+rats+and+hamsters.&rft.au=Lijinsky%2C+W%3BSaavedra%2C+JE%3BKovatch%2C+R+M&rft.aulast=Lijinsky&rft.aufirst=W&rft.date=1988-01-01&rft.volume=42&rft.issue=1-2&rft.spage=37&rft.isbn=&rft.btitle=&rft.title=Cancer+Letters&rft.issn=03043835&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - carcinogenesis ER - TY - JOUR T1 - Inactivity of fecapentaene-12 as a rodent carcinogen or tumor initiator. AN - 15443891; 2179165 AB - The possible carcinogenic activity of synthetic fecapentaene-12 (FP-12) was studied in several mammalian test systems: for carcinogenicity by intrarectal instillation in male F344/NCr rats as well as by intrarectal and subcutaneous application in male B6C3F1 mice; for initiation by skin painting in female SENCAR mice followed by repeated applications of 12-O-tetradecanoylphorbol-13-acetate (TPA), with 7,12-dimethylbenz(a)anthracene (DMBA) followed by TPA as positive control; in a rat subcutaneous granuloma pouch assay in which mutagenicity was measured by induction of 6-thioguanine (6-TG) resistance and carcinogenicity was determined by induction of subcutaneous tumors in the pouch. There was no significant increase in tumor incidence after 72-78 weeks, although 2 rats receiving FP-12 intrarectally developed colon polyps. JF - Cancer Letters AU - Ward, J M AU - Anjo, T AU - Ohannesian, L AU - Keefer, L K AU - Devor, DE AU - Donavan, P J AU - Smith, G T AU - Henneman, J R AU - Streeter, A J AD - NCI-FCRF, Build. 538, Frederick, MD 21701-1013, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 49 EP - 59 VL - 42 IS - 1-2 SN - 0304-3835, 0304-3835 KW - fecapentaene-12 KW - rats KW - mice KW - Toxicology Abstracts KW - carcinogenicity KW - X 24120:Food, additives & contaminants UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15443891?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Letters&rft.atitle=Inactivity+of+fecapentaene-12+as+a+rodent+carcinogen+or+tumor+initiator.&rft.au=Ward%2C+J+M%3BAnjo%2C+T%3BOhannesian%2C+L%3BKeefer%2C+L+K%3BDevor%2C+DE%3BDonavan%2C+P+J%3BSmith%2C+G+T%3BHenneman%2C+J+R%3BStreeter%2C+A+J&rft.aulast=Ward&rft.aufirst=J&rft.date=1988-01-01&rft.volume=42&rft.issue=1-2&rft.spage=49&rft.isbn=&rft.btitle=&rft.title=Cancer+Letters&rft.issn=03043835&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - carcinogenicity ER - TY - JOUR T1 - Genetics and biological markers of risk for alcoholism. AN - 15286446; 2044003 AB - Substantial scientific evidence has accumulated that both genetic and environmental factors predispose the development of alcoholism in certain individuals. Evidence has accumulated to indicate that alcoholism is a heterogeneous entity arising from multiple etiologies. The demonstrated role of genetics in increasing the risk of alcoholism has promoted the search for biological markers that could objectively identify individuals who are genetically predisposed to alcoholism. Identifying such markers could allow for early diagnosis, focused prevention, and differential and type-specific treatment of alcoholism. Promising markers have been provided by research in electrophysiology, endocrinology, and biochemistry. JF - Public Health Reports AU - Tabakoff, B AU - Hoffman, P L AD - NIAAA Intramural Res. Program, NIH Clin. Cent., Rm. 10/3c-103, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 690 EP - 698 VL - 103 IS - 6 SN - 0033-3549, 0033-3549 KW - biology KW - alcoholism KW - aetiology KW - Health & Safety Science Abstracts KW - genetics KW - public health KW - H SM10.20:ALCOHOLISM KW - H SM5.8.4:DRUGS AND ALCOHOL UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15286446?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Public+Health+Reports&rft.atitle=Genetics+and+biological+markers+of+risk+for+alcoholism.&rft.au=Tabakoff%2C+B%3BHoffman%2C+P+L&rft.aulast=Tabakoff&rft.aufirst=B&rft.date=1988-01-01&rft.volume=103&rft.issue=6&rft.spage=690&rft.isbn=&rft.btitle=&rft.title=Public+Health+Reports&rft.issn=00333549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - genetics; public health ER - TY - JOUR T1 - Preliminary crystallographic study of a retroviral protease. AN - 15260404; 2009853 AB - Crystals of Rous sarcoma virus protease, suitable for X-ray structural analysis, were obtained by the method of vapor diffusion using ammonium sulfate as the precipitant. The protease crystallized in the space group P3 sub(1)21 (a = b = 88.8 angstrom, c = 78.8 angstrom) with two molecules in the asymmetric unit. Native data have been collected to 2 multiplied by 5 resolution and the search for heavy-atom derivatives is in progress. JF - Journal of Molecular Biology AU - Miller, M AU - Leis, J AU - Wlodawer, A AD - Crystallogr. Lab., NCI-FCRF, BRI-Basic Res. Program, P.O. Box B, Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 211 EP - 212 VL - 204 IS - 1 SN - 0022-2836, 0022-2836 KW - Rous sarcoma virus KW - X-ray crystallography KW - proteinase KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - V 22189:Miscellaneous topics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15260404?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Molecular+Biology&rft.atitle=Preliminary+crystallographic+study+of+a+retroviral+protease.&rft.au=Miller%2C+M%3BLeis%2C+J%3BWlodawer%2C+A&rft.aulast=Miller&rft.aufirst=M&rft.date=1988-01-01&rft.volume=204&rft.issue=1&rft.spage=211&rft.isbn=&rft.btitle=&rft.title=Journal+of+Molecular+Biology&rft.issn=00222836&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Primary structure of rat chromogranin A and distribution of its mRNA. AN - 15257138; 2014861 AB - The primary structure of rat chromogranin A has been deduced from a rat adrenal cDNA clone. A comparison of rat and bovine chromogranin A reveals several similar features: clusters of polyglutamic acid, similar amino acid composition, position of seven of 10 pairs of basic amino acids, identical placement of the only two cysteine residues, a highly conserved N- and C-terminus, and a sequence homologous to porcine pancreastatin 1-49. Unique features of rat chromogranin A are an eicosaglutamine sequence and two potential N-linked glycosylation sites. JF - FEBS Letters AU - Iacangelo, A AU - Okayama, H AU - Eiden, LE AD - Lab. Cell Biol., Build. 36, Rm. 3A-17, NIMH, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 115 EP - 121 VL - 227 IS - 2 SN - 0014-5793, 0014-5793 KW - adrenal medulla KW - amino acid sequence KW - chromogranin A KW - predictions KW - rats KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15257138?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=Primary+structure+of+rat+chromogranin+A+and+distribution+of+its+mRNA.&rft.au=Iacangelo%2C+A%3BOkayama%2C+H%3BEiden%2C+LE&rft.aulast=Iacangelo&rft.aufirst=A&rft.date=1988-01-01&rft.volume=227&rft.issue=2&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - The importance of the phenyl-tropholone "aS" configuration in colchicine's binding to tubulin. AN - 15255893; 2014388 AB - Measuring ellipticities of ( plus or minus )-colchicine and ( plus or minus )-deacetamidocolchicine in the presence of tubulin afforded net positive CD bands with maxima at 340 nm resulting from reduction of the negative ellipticities upon binding of (-) enantiomers to the protein. Results of optical studies together with earlier NMR conformational analysis of these molecules substantiate the hypothesis that colchicinoids bind to tubulin with the phenyl-tropolone moiety in the "aS" configuration. Natural colchicine which binds to tubulin, therefore, should be referred to as (-)-(aS,7S)-colchicine. JF - FEBS Letters AU - Yeh, HJC AU - Chranowska, M AU - Brossi, A AD - Lab. Anal. Chem., NIDDK, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 82 EP - 86 VL - 229 IS - 1 SN - 0014-5793, 0014-5793 KW - C.D. KW - albumin KW - binding KW - colchicine KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15255893?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=The+importance+of+the+phenyl-tropholone+%22aS%22+configuration+in+colchicine%27s+binding+to+tubulin.&rft.au=Yeh%2C+HJC%3BChranowska%2C+M%3BBrossi%2C+A&rft.aulast=Yeh&rft.aufirst=HJC&rft.date=1988-01-01&rft.volume=229&rft.issue=1&rft.spage=82&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Recognition of the P1 plasmid centromere analog involves binding of the ParB protein and is modified by a specific host factor. AN - 15231590; 1996397 AB - The P1 plasmid partition system is responsible for segregation of daughter plasmids during division of the Escherichia coli host cell. The P1-encoded elements consists of two essential proteins, ParA and ParB, and the cis-acting inc B region. The inc B region determines partition-mediated incompatibility and contains the centromere-like site par S. The authors have isolated and purified the two proteins. ParB binds specifically to the inc B region in vitro. Information for host-factor binding lies in the region determining the specificity of plasmid incompatibility. The roles of parB and the host factor in partition and the specificity of plasmid incompatibility are discussed. JF - EMBO Journal AU - Davis, MA AU - Austin, S J AD - Lab. Chromosome Biol., BRI-Basic Res. Program, NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 1881 EP - 1888 VL - 7 IS - 6 SN - 0261-4189, 0261-4189 KW - P1 plasmid KW - partition KW - systems KW - centromeres KW - Escherichia coli KW - Biochemistry Abstracts 2: Nucleic Acids; Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - DNA-binding protein KW - plasmids KW - N 14930:Transcription factors KW - J 02760:Plasmids KW - G 07200:P PLASMIDS UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15231590?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=EMBO+Journal&rft.atitle=Recognition+of+the+P1+plasmid+centromere+analog+involves+binding+of+the+ParB+protein+and+is+modified+by+a+specific+host+factor.&rft.au=Davis%2C+MA%3BAustin%2C+S+J&rft.aulast=Davis&rft.aufirst=MA&rft.date=1988-01-01&rft.volume=7&rft.issue=6&rft.spage=1881&rft.isbn=&rft.btitle=&rft.title=EMBO+Journal&rft.issn=02614189&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - DNA-binding protein; plasmids ER - TY - JOUR T1 - Retinal degeneration in the mouse induced transplacentally by N-methyl-N-nitrosourea: Effects of constant illumination or total darkness. AN - 15230296; 1987108 AB - The DNA alkylating agent, N-methyl-N-nitrosourea (MNU), when administered prenatally (on day 16 of gestation) provokes a progressive retinal degeneration in CD-1 albino mice reared in standard fluorescent lighting conditions (12 L : 12 D). This degeneration begins at about 4 weeks postnatally and worsens with age. To determine whether light was essential to the development of this lesion, animals were maintained in either constant light or constant darkness. Systematic measurement of the inner and outer segment lengths, the number of rows of photoreceptor cells and the thickness of the outer nuclear layer and whole retina were made to quantify degenerative changes in animals at 2-, 4-, 6-, 8-, 12-, and 16 weeks of age. The constant light caused a drastic reduction in thickness of the retinas of MNU-treated and control mice. The MNU-exposed animals reared in the dark did not demonstrate this reduction in retinal thickness, at least up to 16 weeks of age. JF - Experimental Cell Research AU - Smith, S B AU - Hashimi, W AU - Yielding, K L AD - Build. 6, Rm. B1-A04, NEI, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 347 EP - 359 VL - 47 IS - 3 SN - 0014-4827, 0014-4827 KW - N-methyl-N-nitrosourea KW - degeneration KW - mice KW - Toxicology Abstracts KW - prenatal experience KW - light KW - retina KW - X 24200:Nitrosamines & related compounds UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15230296?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+Cell+Research&rft.atitle=Retinal+degeneration+in+the+mouse+induced+transplacentally+by+N-methyl-N-nitrosourea%3A+Effects+of+constant+illumination+or+total+darkness.&rft.au=Smith%2C+S+B%3BHashimi%2C+W%3BYielding%2C+K+L&rft.aulast=Smith&rft.aufirst=S&rft.date=1988-01-01&rft.volume=47&rft.issue=3&rft.spage=347&rft.isbn=&rft.btitle=&rft.title=Experimental+Cell+Research&rft.issn=00144827&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - retina; prenatal experience; light ER - TY - JOUR T1 - Synthesis and anticholinesterase activity of (-)-N super(1)-Norphysostigmine, (-)-eseramine, and other N(1)-substituted analogues of (-)-physostigmine. AN - 15226247; 1989625 AB - (-)-N super(1)-Benzylnorphysostigmine, prepared from synthetic (-)-O-methyl-N super(1)-noreseroline by N-benzylation, ether cleavage, and reaction of (-)-N super(1)-benzylnoreseroline with methyl isocyanate, was the intermediate used to prepare the title compounds. JF - Journal of Medicinal Chemistry AU - Yu, Qian-Sheng AU - Atack, J R AU - Rapoport, SI AU - Brossi, A AD - Med. Chem. Sect., Lab. Anal. Chem., NIDDK, Lab. Neurosci., NIA, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 2297 EP - 2300 VL - 31 IS - 12 SN - 0022-2623, 0022-2623 KW - activity KW - analogs KW - cholinesterase KW - inhibitors KW - physostigmine KW - synthesis KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15226247?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Synthesis+and+anticholinesterase+activity+of+%28-%29-N+super%281%29-Norphysostigmine%2C+%28-%29-eseramine%2C+and+other+N%281%29-substituted+analogues+of+%28-%29-physostigmine.&rft.au=Yu%2C+Qian-Sheng%3BAtack%2C+J+R%3BRapoport%2C+SI%3BBrossi%2C+A&rft.aulast=Yu&rft.aufirst=Qian-Sheng&rft.date=1988-01-01&rft.volume=31&rft.issue=12&rft.spage=2297&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Oligomeric structure of p21 ras proteins as determined by radiation inactivation. AN - 15199645; 1968954 AB - Using radiation inactivation the authors determined that p21 ras proteins exhibit an oligomeric target size when assayed both structurally and functionally. Similar target sizes of p21 in ras-transformed cells and in purified preparations of the protein suggested that its structure is homo-oligomeric. p21 monomers were destroyed by radiation with the same target size as the GTP binding activity, indicating the occurrence of a tight association allowing energy transfer between the monomers. JF - Journal of Biological Chemistry AU - Santos, E AU - Nebreda, A R AU - Bryan, T AU - Kempner, E S AD - Lab. Mol. Microbiol., NIAID, NIH, Build. 5, Rm. B1-26, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 9853 EP - 9858 VL - 263 IS - 20 SN - 0021-9258, 0021-9258 KW - genes KW - inactivation KW - mice KW - oligomeric KW - oncogenes KW - protein p21 KW - radiation KW - ras gene KW - structure KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - G 07398:GENERAL UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15199645?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Oligomeric+structure+of+p21+ras+proteins+as+determined+by+radiation+inactivation.&rft.au=Santos%2C+E%3BNebreda%2C+A+R%3BBryan%2C+T%3BKempner%2C+E+S&rft.aulast=Santos&rft.aufirst=E&rft.date=1988-01-01&rft.volume=263&rft.issue=20&rft.spage=9853&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - radiation; genes ER - TY - JOUR T1 - Neurotoxic damage to the nigrostriatal system in rats following intranigral administration of MPDP super(+) and MPP super(+). AN - 15195374; 1975842 AB - Unilateral intranigral administration of the oxidative metabolites of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenyl-dihydropyridine (MPDP super(+)) or 1-methyl-4-phenylpyridine (MPP super(+)) produced dose-dependently a depletion of dopamine in the ipsilateral striatum of rats two weeks following treatment. MPP super(+) increased the super(45)Ca accumulation into cells at the site of injection and produced "nonspecific" cell membrane and/or cytotoxic damage seen by histological procedures. The results indicate that MPDP super(+) and MPP super(+) produced localized cytotoxic damage to nigrostriatal neurons, caused a decrease in striatal dopamine, and disrupted the nigrostriatal system's functioning following intranigral administration to rats. JF - Journal of Neural Transmission AU - Sun, C J AU - Johannessen, J N AU - Gessner, W AU - Namura, I AU - Singhaniyom, W AU - Brossi, A AU - Chiueh, C C AD - Lab. Cerebral Metab., NIMH, NIH, Build. 10, Rm. 2 D-52, Bethesda, MD 20892-1000, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 75 EP - 86 VL - 74 IS - 2 SN - 0300-9564, 0300-9564 KW - MPP super(+) KW - administration KW - effects on KW - rats KW - comparison KW - MPDP super(+) KW - Toxicology Abstracts; CSA Neurosciences Abstracts KW - neurotoxicity KW - substantia nigra KW - neostriatum KW - N3 11104:Mammals (except primates) KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15195374?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aabiglobal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=Political+Science+Quarterly&rft.atitle=The+Political+and+Economic+Forces+Shaping+Concentrated+Poverty&rft.au=Wilson%2C+William+Julius&rft.aulast=Wilson&rft.aufirst=William&rft.date=2008-12-01&rft.volume=123&rft.issue=4&rft.spage=555&rft.isbn=&rft.btitle=&rft.title=Political+Science+Quarterly&rft.issn=00323195&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - substantia nigra; neurotoxicity; neostriatum ER - TY - JOUR T1 - A perspective on science and public health policy. AN - 15189364; 1966579 JF - Public Health Reports AU - Gordis, E AD - NIAAA, Rm. 16-105 Parklawn Build., 5600 Fishers Lane, Rockville, MD 20857, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 575 EP - 577 VL - 103 IS - 6 SN - 0033-3549, 0033-3549 KW - public health KW - research and development KW - alcoholism KW - Health & Safety Science Abstracts KW - training KW - federal policies KW - H SM3.5:STANDARDS, LAWS, REGULATIONS, AND POLICY UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15189364?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Public+Health+Reports&rft.atitle=A+perspective+on+science+and+public+health+policy.&rft.au=Gordis%2C+E&rft.aulast=Gordis&rft.aufirst=E&rft.date=1988-01-01&rft.volume=103&rft.issue=6&rft.spage=575&rft.isbn=&rft.btitle=&rft.title=Public+Health+Reports&rft.issn=00333549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - training; federal policies ER - TY - JOUR T1 - Screening for alcoholism: Techniques and issues. AN - 15189170; 1966550 AB - Alcoholism is an often overlooked health problem because alcoholics usually do not seek treatment for their drinking problems. They do, however, seek general medical care for other health reasons, and a number of screening techniques have proven useful for identifying alcoholics. The advantages and disadvantages of self-report, as well as biochemical techniques that have been found effective in screening for alcoholism, are discussed. JF - Public Health Reports AU - Allen, J P AU - Eckardt, MJ AU - Wallen, J AD - NIAAA, Treat. Res. Branch, Parklawn 16-C03, Rockville, MD 20857, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 586 EP - 592 VL - 103 IS - 6 SN - 0033-3549, 0033-3549 KW - alcoholism KW - screening KW - research and development KW - Health & Safety Science Abstracts KW - H SM10.20:ALCOHOLISM UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15189170?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Public+Health+Reports&rft.atitle=Screening+for+alcoholism%3A+Techniques+and+issues.&rft.au=Allen%2C+J+P%3BEckardt%2C+MJ%3BWallen%2C+J&rft.aulast=Allen&rft.aufirst=J&rft.date=1988-01-01&rft.volume=103&rft.issue=6&rft.spage=586&rft.isbn=&rft.btitle=&rft.title=Public+Health+Reports&rft.issn=00333549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 ER - TY - JOUR T1 - Alcoholism treatment service systems: A health services research perspective. AN - 15186537; 1966526 AB - This article examines the role of health services research in alcoholism treatment. Alcoholism services research has only recently emerged as a self-defined discipline. Alcoholism services research can be grouped into five classifications: a) descriptive studies of resources for alcoholism treatment and of the use or cost of these services, b) estimates of the need or demand for alcohol services in the population or in particular subpopulations, c) studies of the costs or cost-effectiveness of alcoholism treatment or of alternative treatments, d) studies of the possible "cost-offsets" of treating alcoholism, and e) studies that examine strategies for financing and reimbursement for alcoholism treatment. JF - Public Health Reports AU - Wallen, J AD - NIAAA, Parklawn Build., Rm. 16C-05, Rockville, MD 20852, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 605 EP - 611 VL - 103 IS - 6 SN - 0033-3549, 0033-3549 KW - alcoholism KW - cost benefit analysis KW - Health & Safety Science Abstracts KW - economics KW - public health KW - H SM10.20:ALCOHOLISM UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15186537?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Public+Health+Reports&rft.atitle=Alcoholism+treatment+service+systems%3A+A+health+services+research+perspective.&rft.au=Wallen%2C+J&rft.aulast=Wallen&rft.aufirst=J&rft.date=1988-01-01&rft.volume=103&rft.issue=6&rft.spage=605&rft.isbn=&rft.btitle=&rft.title=Public+Health+Reports&rft.issn=00333549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - economics; public health ER - TY - JOUR T1 - Cortisol response to clonidine in panic disorder: Comparison with depressed patients and normal controls. AN - 15186499; 1967122 AB - Abnormalities in regulation of noradrenergic function have been proposed as part of the pathology of depressive and panic anxiety disorders. However, abnormalities in hypothalamic-pituitary-adrenal (HPA) axis function have largely been limited to patients with depressive disorders. Using the cortisol response to clonidine, an alpha sub(2)-adrenergic receptor agonist, this study examined the relationship between the noradrenergic system and the HPA axis in 10 patients with major depression (4 unipolar, 6 bipolar), 10 patients with panic disorder, and 10 normal controls. Baseline cortisol was significantly elevated in depressed as compared with panic patients, but not with controls. JF - Biological Psychiatry AU - Stein, M B AU - Uhde, T W AD - Unit Anxiety and Affective Disord., BPB, NIMH, Build. 10, Rm 3S239, 9000 Rockville Pike, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 322 EP - 330 VL - 24 IS - 3 SN - 0006-3223, 0006-3223 KW - panic disorders KW - cortisol KW - clonidine KW - Health & Safety Science Abstracts KW - psychology KW - H SM9.7:HUMAN FACTORS UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15186499?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biological+Psychiatry&rft.atitle=Cortisol+response+to+clonidine+in+panic+disorder%3A+Comparison+with+depressed+patients+and+normal+controls.&rft.au=Stein%2C+M+B%3BUhde%2C+T+W&rft.aulast=Stein&rft.aufirst=M&rft.date=1988-01-01&rft.volume=24&rft.issue=3&rft.spage=322&rft.isbn=&rft.btitle=&rft.title=Biological+Psychiatry&rft.issn=00063223&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - psychology ER - TY - JOUR T1 - A heat-shock-inducible eukaryotic expression vector. AN - 15178571; 1956899 AB - The authors describe a construct, pHS/Cla, containing the Drosophila melanogaster hsp70 promoter which serves as an inducible expression vector in mammalian cells. The construct was made in the plasmid pAT153, a derivative of pBR322. In transient transfections of human H9 T-cells, the transactivator of transcription protein of the human AIDS virus HIV-1 was functionally expressed in response to heat shock. The promoter is very tightly regulated in that no expression can be detected at 37 degree C. The construct contains a unique ClaI site for cloning and expressing potentially any gene. JF - Gene AU - Schweinfest, C W AU - Jorcyk, CL AU - Fujiwara, S AU - Papas, T S AD - Lab. Mol. Oncol., NCI, Frederick, MD 21701-1013, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 207 EP - 210 VL - 71 IS - 1 SN - 0378-1119, 0378-1119 KW - hsp70 gene KW - AIDS KW - immunodeficiency virus 1 (human) KW - pHS/Cla plasmid KW - derivatives KW - pAT153 plasmid KW - pBR322 plasmid KW - transactivator protein KW - expression KW - cloning vectors KW - genes KW - plasmids KW - promoters KW - Biotechnology and Bioengineering Abstracts; Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - W 30114:Cloning vectors KW - N 14682:Cloning vectors KW - G 07200:P PLASMIDS UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15178571?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene&rft.atitle=A+heat-shock-inducible+eukaryotic+expression+vector.&rft.au=Schweinfest%2C+C+W%3BJorcyk%2C+CL%3BFujiwara%2C+S%3BPapas%2C+T+S&rft.aulast=Schweinfest&rft.aufirst=C&rft.date=1988-01-01&rft.volume=71&rft.issue=1&rft.spage=207&rft.isbn=&rft.btitle=&rft.title=Gene&rft.issn=03781119&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - genes; promoters; plasmids; cloning vectors ER - TY - JOUR T1 - Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity. AN - 15146692; 1920562 AB - Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity. JF - FEBS Letters AU - Shai, Y AU - Fox, J AU - Caratsch, C AU - Shih, Yu-Liang AU - Edwards, C AU - Lazarovici, P AD - Lab. Cell Biol. and Genet., NIDDK, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 161 EP - 166 VL - 242 IS - 1 SN - 0014-5793, 0014-5793 KW - Pardachirus marmoratus KW - amino acid sequence KW - neurotoxins KW - new products KW - pardaxin KW - peptide synthesis KW - polypeptides KW - synthetic pardaxin KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology; ASFA 1: Biological Sciences & Living Resources KW - Marine KW - Q1:08524 KW - Q1 08625:Non-edible products UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15146692?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=Sequencing+and+synthesis+of+pardaxin%2C+a+polypeptide+from+the+Red+Sea+Moses+sole+with+ionophore+activity.&rft.au=Shai%2C+Y%3BFox%2C+J%3BCaratsch%2C+C%3BShih%2C+Yu-Liang%3BEdwards%2C+C%3BLazarovici%2C+P&rft.aulast=Shai&rft.aufirst=Y&rft.date=1988-01-01&rft.volume=242&rft.issue=1&rft.spage=161&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - neurotoxins; polypeptides; new products; Marine ER - TY - JOUR T1 - The P1 plasmid-partition system synthesizes two essential proteins from an autoregulated operon. AN - 15145429; 1934578 AB - The P1 partition region contains two large open reading frames that encode the proteins ParA and ParB. It was previously shown that ParA is essential for partition activity. Using a novel assay, the authors show that ParB protein is also an absolute requirement for partition and that it is active in trans to the partitioning plasmid. Development of complementation tests for parA and parB allow them to assign a number of partition-defective point mutants of a P1 miniplasmid to the parA and parB cistrons. Using gene fusion techniques, it was shown that parA and parB constitute and operon controlled from a promoter proximal to the star of parA . JF - Plasmid AU - Friedman, SA AU - Austin, S J AD - Lab. Chromosome Biol., BRI-Basic Res. Program, NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 103 EP - 112 VL - 19 IS - 2 SN - 0147-619X, 0147-619X KW - plasmid P1 KW - genes KW - ParB protein KW - requirements KW - partioning KW - ParA protein KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - plasmids KW - J 02760:Plasmids KW - G 07200:P PLASMIDS KW - N 14662:Gene regulation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15145429?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Plasmid&rft.atitle=The+P1+plasmid-partition+system+synthesizes+two+essential+proteins+from+an+autoregulated+operon.&rft.au=Friedman%2C+SA%3BAustin%2C+S+J&rft.aulast=Friedman&rft.aufirst=SA&rft.date=1988-01-01&rft.volume=19&rft.issue=2&rft.spage=103&rft.isbn=&rft.btitle=&rft.title=Plasmid&rft.issn=0147619X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - plasmids ER - TY - JOUR T1 - Receptors with V sub(1) characteristics mediate the maintenance of ethanol tolerance by vasopressin. AN - 15137870; 1928053 AB - The neurohypohyseal hormone arginine vasopressin (AVP) acts in the central nervous system (CNS) to maintain functional tolerance to several effects of ethanol. The ability of exogenous vasopressin (administered i.c.v.) to maintain tolerance to the hypnotic effect was blocked more effectively by antagonists acting at V sub(1) receptors than by a V sub(2)-selective antagonist. JF - Journal of Pharmacology and Experimental Therapeutics AU - Szabo, G AU - Tabakoff, B AU - Hoffman, P L AD - NIAAA/LPPS, 12501 Washington Ave., Rockville, MD 20852, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 536 EP - 541 VL - 247 IS - 2 SN - 0022-3565, 0022-3565 KW - vasopressin V1 KW - receptors KW - mediation KW - laboratory animals KW - ethanol KW - Toxicology Abstracts; CSA Neurosciences Abstracts KW - brain KW - drug tolerance KW - N3 11104:Mammals (except primates) KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15137870?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Pharmacology+and+Experimental+Therapeutics&rft.atitle=Receptors+with+V+sub%281%29+characteristics+mediate+the+maintenance+of+ethanol+tolerance+by+vasopressin.&rft.au=Szabo%2C+G%3BTabakoff%2C+B%3BHoffman%2C+P+L&rft.aulast=Szabo&rft.aufirst=G&rft.date=1988-01-01&rft.volume=247&rft.issue=2&rft.spage=536&rft.isbn=&rft.btitle=&rft.title=Journal+of+Pharmacology+and+Experimental+Therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - drug tolerance; brain ER - TY - JOUR T1 - Toxic agent and radiation control: Progress toward objectives for the nation for the year 1990. AN - 15135006; 1918527 AB - In 1980, the Department of Health and Human Services set national prevention objectives for 1990 in 15 health priority areas, 1 of which is the control of toxic agents and radiation. Ten objectives related to this area are priorities for the national control effort. progress is reviewed on those priorities within the responsibilities of the Public Health Service. JF - Public Health Reports AU - Rall, D P AD - Off. Program Plann. and Eval., NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 342 EP - 347 VL - 103 IS - 4 SN - 0033-3549, 0033-3549 KW - Health & Safety Science Abstracts; Pollution Abstracts KW - radiation KW - toxic materials KW - environmental protection KW - public health KW - pollution control KW - H SM3.1:BASIC APPROACHES, CONCEPTS, AND THEORY KW - P 9000:ENVIRONMENTAL ACTION UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15135006?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Public+Health+Reports&rft.atitle=Toxic+agent+and+radiation+control%3A+Progress+toward+objectives+for+the+nation+for+the+year+1990.&rft.au=Rall%2C+D+P&rft.aulast=Rall&rft.aufirst=D&rft.date=1988-01-01&rft.volume=103&rft.issue=4&rft.spage=342&rft.isbn=&rft.btitle=&rft.title=Public+Health+Reports&rft.issn=00333549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - toxic materials; radiation; pollution control; public health; environmental protection ER - TY - JOUR T1 - Inhibitory effect of zinc on nickel subsulfide carcinogenesis in Fischer rats. AN - 15128845; 1917967 AB - The effects of zinc oxide (ZnO) and zinc acetate (ZnAcet) administered i.m. together with nickel subsulfide (Ni sub(3)S sub(2)), a potent muscle carcinogen, were observed over 66 weeks in male F344/NCr rats. In rats given Ni sub(3)S sub(2) alone the incidence of local tumors reached 100% in 40 weeks. In rats treated locally with Ni sub(3)S sub(2) + ZnO or znAcet, the tumor incidence at week 40 was only 40-60%; it reached 85-100% in 66 weeks, with no significant differences among the treatments. Treatment with i.m. Ni sub(3)S sub(2) + s.c. ZnO resulted in 100% muscle tumors at week 58. One local tumor was found in rats given ZnO alone and none in the water injected animals. Statistical analysis revealed highly significant differences in the tumor occurrence rates between rats treated with Ni sub(3)S sub(2) alone and rats treated with Ni sub(3)S sub(2) combined with ZnO or ZnAcet, whereas the final tumor incidences at week 66 were not different. The first tumors were found at weeks 24-31 regardless of the treatment. Hence, administration of zinc slows the carcinogenic process induced by nickel. JF - Toxicology AU - Kasprzak, K S AU - Kovatch, R M AU - Poirier, LA AD - Bldg. 538, Rm. 205, NCI-FCRF, Frederick, MD 21701-1013, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 253 EP - 262 VL - 52 IS - 3 SN - 0300-483X, 0300-483X KW - nickel subsulfide KW - zinc KW - rats KW - heavy metals KW - Health & Safety Science Abstracts; Pollution Abstracts; Toxicology Abstracts KW - carcinogenesis KW - H SE4.20:POISONS AND POISONING KW - X 24162:Chronic exposure KW - P 6000:TOXICOLOGY AND HEALTH UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15128845?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=Inhibitory+effect+of+zinc+on+nickel+subsulfide+carcinogenesis+in+Fischer+rats.&rft.au=Kasprzak%2C+K+S%3BKovatch%2C+R+M%3BPoirier%2C+LA&rft.aulast=Kasprzak&rft.aufirst=K&rft.date=1988-01-01&rft.volume=52&rft.issue=3&rft.spage=253&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - zinc; carcinogenesis; rats; heavy metals ER - TY - JOUR T1 - Stimulation of phosphoribosyl pyrophosphate and purine nucleotide production by pyrroline 5-carboxylate in human erythrocytes. AN - 15125734; 1915964 AB - Recent studies have shown that pyrroline 5-carboxylate, the intermediate in the interconversions of proline, ornithine, and glutamate, can regulate the metabolism of erythrocytes. The authors report that the formation of 5-phosphoribosyl 1-pyrophosphate (PP-Rib-P) was markedly stimulated by pyrroline 5-carboxylate in intact red cells. The production of PP-Rib-P is an important point of regulation in nucleotide metabolism. The authors now report that pyrroline 5-carboxylate markedly stimulated the net synthesis of inosine monophosphate from hypoxanthine in intact human red cells so that the pool of inosine monophosphate became 20-30% of the total pool of purine nucleotides. JF - Journal of Biological Chemistry AU - Yeh, Grace Chao AU - Phang, James M AD - Build. 10, Rm. 4N117, NCI, NIH, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 13083 EP - 13089 VL - 263 IS - 26 SN - 0021-9258, 0021-9258 KW - biosynthesis KW - erythrocytes KW - man KW - phosphoribosyl pyrophosphate KW - purine nucleotides KW - pyrroline 5-carboxylate KW - stimulation KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15125734?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Stimulation+of+phosphoribosyl+pyrophosphate+and+purine+nucleotide+production+by+pyrroline+5-carboxylate+in+human+erythrocytes.&rft.au=Yeh%2C+Grace+Chao%3BPhang%2C+James+M&rft.aulast=Yeh&rft.aufirst=Grace&rft.date=1988-01-01&rft.volume=263&rft.issue=26&rft.spage=13083&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Basal ppGpp level adjustment shown by new spoT mutants affect steady state growth rates and rrnA ribosomal promoter regulation in Escherichia coli . AN - 15124323; 1913379 AB - This work describes an approach towards analyzing the regulatory effects of variation of guanosine 3',5'-bispyrophosphate (ppGpp) basal levels in Escherichia coli during steady state growth. A series of strains was derived by mutating the spoT gene (which encodes the major cellular ppGppase) so as to obtain systematic increments in ppGpp basal levels. Measurements of ppGpp revealed a ten-fold range of basal levels during growth on minimal medium. The empirical relationship between ppGpp concentration and growth rate is a simple linear inverse correlation. We conclude that there are systematic regulatory consequences associated with small changes in ppGpp basal levels during steady state growth that probably are part of a continuum with more dramatic effects observed during the stringent response to amino acid deprivation. JF - Molecular and General Genetics AU - Sarubbi, E AU - Rudd, KE AU - Cashel, M AD - Build. 6, Rm. 335, NICHD, NIH, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 214 EP - 222 VL - 213 IS - 2-3 SN - 0026-8925, 0026-8925 KW - growth rate KW - spoT gene KW - regulation KW - rrnA gene KW - promoters KW - mutants KW - effects on KW - levels KW - guanosine tetraphosphate KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - genes KW - Escherichia coli KW - G 07320:Bacterial genetics KW - N 14662:Gene regulation KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15124323?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+General+Genetics&rft.atitle=Basal+ppGpp+level+adjustment+shown+by+new+spoT+mutants+affect+steady+state+growth+rates+and+rrnA+ribosomal+promoter+regulation+in+Escherichia+coli+.&rft.au=Sarubbi%2C+E%3BRudd%2C+KE%3BCashel%2C+M&rft.aulast=Sarubbi&rft.aufirst=E&rft.date=1988-01-01&rft.volume=213&rft.issue=2-3&rft.spage=214&rft.isbn=&rft.btitle=&rft.title=Molecular+and+General+Genetics&rft.issn=00268925&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - Escherichia coli; genes ER - TY - JOUR T1 - Rapid development of hepatocellular neoplasms in aging male C3H/HeNCr mice given phenobarbital. AN - 15118533; 1903275 AB - One hundred and nineteen male C3H/HeNCr mice, 12 months of age, with spontaneous preneoplastic and neoplastic hepatocellular lesions were given phenobarbital (PB) at 500 ppm in drinking water. In aging mice, PB exposure significantly increased the number of gross tumors or microscopic foci, adenomas or carcinomas per liver at all time periods, especially unique eosinophilic proliferative lesions, while young mice did not develop any focal proliferative lesions by 36 weeks. The findings suggest that in aging mice a fraction of the hepatocyte population (normal, spontaneously-initiated or preneoplastic) is more highly susceptible to phenobarbital "carcinogenesis" than are hepatocytes of younger mice. JF - Cancer Letters AU - Ward, J M AU - Lynch, P AU - Riggs, C AD - Tumor Pathol. and Pathogenesis Sect., Lab. Comp. Carcinogenesis, Natl. Cancer Inst., NCI-Frederick Cancer Res. Facil., Frederick, MD 21701-1013, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 9 EP - 18 VL - 39 IS - 1 SN - 0304-3835, 0304-3835 KW - phenobarbital KW - mice KW - Toxicology Abstracts KW - age KW - liver KW - neoplasia KW - X 24112:Chronic exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15118533?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Letters&rft.atitle=Rapid+development+of+hepatocellular+neoplasms+in+aging+male+C3H%2FHeNCr+mice+given+phenobarbital.&rft.au=Ward%2C+J+M%3BLynch%2C+P%3BRiggs%2C+C&rft.aulast=Ward&rft.aufirst=J&rft.date=1988-01-01&rft.volume=39&rft.issue=1&rft.spage=9&rft.isbn=&rft.btitle=&rft.title=Cancer+Letters&rft.issn=03043835&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - liver; neoplasia; age ER - TY - JOUR T1 - Fetal hippocampal cell suspensions ameliorate behavioral effects of intradentate colchicine in the rat. AN - 15085283; 1881613 AB - Colchicine, a neurotoxin that preferentially destroys dentate gyrus granule cells and mossy fibers, was injected into the hippocampus of adult rats. Three weeks later, the rats were tested for colchicine-induced hypermotility after which they received fetal hippocampal explants. Locomotor activity was retested three weeks later, after which the rats were trained over a period of four weeks on a food-reinforced, spatial, working memory task in an 8-arm radial maze. Fetal hippocampal explants were found to attenuate significantly the colchicine-induced hypermotility and spatial learning deficits. Histological observations showed the presence of surviving hippocampal explants in both the lesioned and the control rat brains, suggesting that the presence of viable implants facilitates the recovery of behavioral function in rats with spatial memory deficits. JF - Brain Research AU - Tandon, P AU - McLamb, R L AU - Novicki, D AU - Shuey, D L AU - Tilson, HA AD - LMIN, NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 241 EP - 248 VL - 473 IS - 21 SN - 0006-8993, 0006-8993 KW - injection KW - pretreatment KW - effects on KW - rats KW - colchicine KW - Toxicology Abstracts; Animal Behavior Abstracts; CSA Neurosciences Abstracts KW - dentate gyrus KW - transplants KW - granule cells KW - hippocampus KW - locomotion KW - Y 25767:Mammals (excluding primates) KW - X 24115:Pathology KW - N3 11132:Anatomical and surgical correlates UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15085283?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+Research&rft.atitle=Fetal+hippocampal+cell+suspensions+ameliorate+behavioral+effects+of+intradentate+colchicine+in+the+rat.&rft.au=Tandon%2C+P%3BMcLamb%2C+R+L%3BNovicki%2C+D%3BShuey%2C+D+L%3BTilson%2C+HA&rft.aulast=Tandon&rft.aufirst=P&rft.date=1988-01-01&rft.volume=473&rft.issue=21&rft.spage=241&rft.isbn=&rft.btitle=&rft.title=Brain+Research&rft.issn=00068993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - granule cells; dentate gyrus; hippocampus; transplants; locomotion ER - TY - JOUR T1 - A metabolite of the carcinogen 7,12-dimethylbenz(a)anthracene that reacts predominantly with adenine residues in DNA. AN - 15081796; 1883617 AB - Four 7,12-dimethylbenz(a)anthracene-deoxyribonucleoside adducts formed in mouse epidermis in vivo arise from the syn dihydrodiol epoxide metabolite of this carcinogen. With the synthetic syn dihydrodiol epoxide it was possible to identify three of these as deoxyadenosine adducts and to establish their structures. These three adducts account for the large majority of DNA adduct arising from this metabolite in vivo. The in vivo metabolite is unusual, therefore, in that it reacts almost exclusively with adenine residues in DNA while most carcinogen metabolites react preferentially with guanine residues. JF - Carcinogenesis AU - Cheng, S C AU - Prakash, A S AU - Pigott, MA AU - Hilton, B D AU - Lee, H AU - Harvey, R G AU - Dipple, A AD - BRI-Basic Res. Program, NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 1721 EP - 1723 VL - 9 IS - 9 SN - 0143-3334, 0143-3334 KW - metabolites KW - interactions KW - 9,10-dimethyl-1,2-benzanthracene KW - adenine KW - Toxicology Abstracts KW - DNA KW - X 24190:Polycyclic hydrocarbons UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15081796?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=A+metabolite+of+the+carcinogen+7%2C12-dimethylbenz%28a%29anthracene+that+reacts+predominantly+with+adenine+residues+in+DNA.&rft.au=Cheng%2C+S+C%3BPrakash%2C+A+S%3BPigott%2C+MA%3BHilton%2C+B+D%3BLee%2C+H%3BHarvey%2C+R+G%3BDipple%2C+A&rft.aulast=Cheng&rft.aufirst=S&rft.date=1988-01-01&rft.volume=9&rft.issue=9&rft.spage=1721&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - DNA ER - TY - JOUR T1 - Genetic locus, primary structure, and chemical synthesis of human immunodeficiency virus protease. AN - 15073968; 1878858 AB - The genetic locus and primary structure of the human immunodeficiency virus (HIV) protease was determined by comparing the data of protein analyses with the published data of the gene analysis. The complete sequence of HIV-1 and HIV-2 protease was synthesized by solid-phase peptide synthesis. The synthetic protease was capable of accurately cleaving synthetic peptide substrates corresponding to know cleavage sites in gag polyproteins of HIV-1, HIV-2, and murine leukemia virus. The chemical synthesis of protease confirms the DNA sequence and provides a means of rapidly producing active protease in substantial quantities for biochemical and physical studies. JF - GENE ANAL. TECH. AU - Copeland, T D AU - Oroszlan, S AD - Lab. Mol. Virol. and Carcinogenesis, BRI-Basic Res. Program, NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 109 EP - 115 VL - 5 IS - 6 KW - activity KW - amino acid sequence KW - correlation KW - genes KW - human immunodeficiency virus 1 KW - human immunodeficiency virus 2 KW - nucleotide sequence KW - peptide synthesis KW - proteinase KW - proteolysis KW - solid phase methods KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - V 22002:AIDS: Molecular and in vitro aspects KW - V 22032:Viral proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15073968?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=GENE+ANAL.+TECH.&rft.atitle=Genetic+locus%2C+primary+structure%2C+and+chemical+synthesis+of+human+immunodeficiency+virus+protease.&rft.au=Copeland%2C+T+D%3BOroszlan%2C+S&rft.aulast=Copeland&rft.aufirst=T&rft.date=1988-01-01&rft.volume=5&rft.issue=6&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=GENE+ANAL.+TECH.&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - nucleotide sequence; peptide synthesis; genes; amino acid sequence; activity ER - TY - JOUR T1 - Chemical and immunological characterization of the 21-kDa ADP-ribosylation factor of adenylate cyclase. AN - 15057192; 1848285 AB - The ADP-ribosylation factor (ARF) is a 21-kDa GTP-binding protein cofactor in the cholera toxin-catalyzed ADP-ribosylation of the stimulatory regulatory subunit of adenylate cyclase. Purified bovine brain ARF was digested with cyanogen bromide, and peptides were purified and sequenced. Approximately 25-30% of the protein was sequenced in this manner. Peptides contained consensus sequences for GTP-binding proteins but were distinct from any of the previously published GTP-binding proteins. JF - Journal of Biological Chemistry AU - Kahn, R A AU - Goddard, C AU - Newkirk, M AD - Build. 37, Rm. 5D-02, NCI, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 8282 EP - 8287 VL - 263 IS - 17 SN - 0021-9258, 0021-9258 KW - ADP-ribosylation factor KW - adenylate cyclase KW - amino acid sequence KW - brain KW - cattle KW - characterization KW - cholera toxin KW - immunochemistry KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15057192?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Chemical+and+immunological+characterization+of+the+21-kDa+ADP-ribosylation+factor+of+adenylate+cyclase.&rft.au=Kahn%2C+R+A%3BGoddard%2C+C%3BNewkirk%2C+M&rft.aulast=Kahn&rft.aufirst=R&rft.date=1988-01-01&rft.volume=263&rft.issue=17&rft.spage=8282&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Menopause and ovarian cancer. AN - 15056007; 1845129 AB - Using data from a study of 296 patients diagnosed in greater Washington, D.C., from 1978 to 1981 with primary epithelial ovarian cancer and 343 patients hospitalized for other conditions, the authors estimated the rate ratios according to various characteristics of the menopause. Menopause induced by hysterectomy with preservation of both ovaries was associated with a 30 per cent reduction in risk of later development of ovarian cancer. Age at natural menopause was not consistently related to risk. Women who used menopausal estrogens showed a 40 per cent decreased risk. JF - American Journal of Epidemiology AU - Hartge, P AU - Hoover, R AU - McGowan, L AU - Lesher, L AU - Norris, HJ AD - NCI, Landow Build., Rm. 3C06, NIH, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 990 EP - 998 VL - 127 IS - 5 SN - 0002-9262, 0002-9262 KW - menopause KW - estrogens KW - Health & Safety Science Abstracts KW - risk assessment KW - epidemiology KW - cancer KW - H SM10.21:CANCER UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15056007?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Epidemiology&rft.atitle=Menopause+and+ovarian+cancer.&rft.au=Hartge%2C+P%3BHoover%2C+R%3BMcGowan%2C+L%3BLesher%2C+L%3BNorris%2C+HJ&rft.aulast=Hartge&rft.aufirst=P&rft.date=1988-01-01&rft.volume=127&rft.issue=5&rft.spage=990&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - cancer; epidemiology; risk assessment ER - TY - JOUR T1 - Dense metaphyseal bands and growth arrest associated with isotretinoin therapy. AN - 15052883; 1856647 AB - A 9-year-old boy, treated with high-dose isotretinoin therapy for fibrodysplasia ossificans progressiva, developed dense metaphyseal bands and growth arrest. Discontinuance of isotretinoin therapy was followed by gradual decrease of metaphyseal bands and resumption of clinical growth. The dense metaphyseal bands may be related to the known action of retinoids as modulators of chondrocyte phenotype and gene expression. JF - AM. J. DIS. CHILD. AU - Marini, J C AU - Hill, S AU - Zasloff, MA AD - Hum. Genet. Branch/NICHD, Build. 10, Rm. 8C-429, 9000 Rockville Pike, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 316 EP - 318 VL - 142 IS - 3 SN - 0002-922X, 0002-922X KW - growth KW - case reports KW - isotretinoin KW - Toxicology Abstracts KW - side effects KW - man KW - dermatological agents KW - X 24113:Side effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15052883?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=AM.+J.+DIS.+CHILD.&rft.atitle=Dense+metaphyseal+bands+and+growth+arrest+associated+with+isotretinoin+therapy.&rft.au=Marini%2C+J+C%3BHill%2C+S%3BZasloff%2C+MA&rft.aulast=Marini&rft.aufirst=J&rft.date=1988-01-01&rft.volume=142&rft.issue=3&rft.spage=316&rft.isbn=&rft.btitle=&rft.title=AM.+J.+DIS.+CHILD.&rft.issn=0002922X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - side effects; man; dermatological agents ER - TY - JOUR T1 - Drug purpura due to surreptitious quinidine intake. AN - 15049057; 1856910 AB - Three patients had recurrent episodes of thrombocytopenia that resembled drug purpura, but the drug history in each case did not support the diagnosis. Although the patients specifically denied taking quinidine, serologic testing with this drug was done because the patients had access to it, and it is the commonest cause of drug purpura. Highly specific quinidine-dependent antiplatelet antibodies were found in the sera of all three patients. After being informed of the laboratory findings, the patients have had no recurrences of purpura. Serologic tests for quinidine- or quinine-dependent antibodies can help elucidate some obscure cases of purpura that may be self-induced. JF - Annals of Internal Medicine AU - Reid, D M AU - Shulman, N R AD - Clin. Hematol. Branch, NIDDK, NIH, Build. 10, 8C101, 9000 Rockville Pike, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 206 EP - 208 VL - 108 IS - 2 SN - 0003-4819, 0003-4819 KW - case reports KW - quinidine KW - Toxicology Abstracts KW - side effects KW - antiarrhythmic agents KW - purpura KW - man KW - X 24113:Side effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15049057?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+Internal+Medicine&rft.atitle=Drug+purpura+due+to+surreptitious+quinidine+intake.&rft.au=Reid%2C+D+M%3BShulman%2C+N+R&rft.aulast=Reid&rft.aufirst=D&rft.date=1988-01-01&rft.volume=108&rft.issue=2&rft.spage=206&rft.isbn=&rft.btitle=&rft.title=Annals+of+Internal+Medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - side effects; purpura; man; antiarrhythmic agents ER - TY - JOUR T1 - Factitious hypoglycemia due to surreptitious administration of insulin: Diagnosis, treatment, and long-term follow-up. AN - 15048090; 1856678 AB - Ten patients had factitious hypoglycemia due to surreptitious insulin injections diagnosed and were followed for up to 15 years (median, 5 years; range, 2 months to 15 years). When available, demonstration of anti-insulin antibodies was the most helpful diagnostic test. Decreased plasma C-peptide levels corroborated the diagnosis. Young women (nine of ten) with knowledge of the medical profession or relative with diabetes mellitus predominated in the sample. Five of the patients had a history of insulin-requiring diabetes mellitus. Two patients eventually committed suicide. JF - Annals of Internal Medicine AU - Grunberger, G AU - Weiner, J L AU - Silverman, R AU - Taylor, S AU - Gorden, P AD - DPB/DEMD/NIDDK, Westwood 626, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 252 EP - 257 VL - 108 IS - 2 SN - 0003-4819, 0003-4819 KW - case reports KW - surreptitious injections KW - insulin KW - Toxicology Abstracts KW - side effects KW - reviews KW - hypoglycemia KW - man KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15048090?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+Internal+Medicine&rft.atitle=Factitious+hypoglycemia+due+to+surreptitious+administration+of+insulin%3A+Diagnosis%2C+treatment%2C+and+long-term+follow-up.&rft.au=Grunberger%2C+G%3BWeiner%2C+J+L%3BSilverman%2C+R%3BTaylor%2C+S%3BGorden%2C+P&rft.aulast=Grunberger&rft.aufirst=G&rft.date=1988-01-01&rft.volume=108&rft.issue=2&rft.spage=252&rft.isbn=&rft.btitle=&rft.title=Annals+of+Internal+Medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - side effects; hypoglycemia; man; reviews ER - TY - JOUR T1 - Induction of rat cytochrome P-450 3 and its mRNA by 3,4,5,3',4',5'-hexachlorobiphenyl. AN - 15029080; 1836888 AB - Rat cytochrome P-450 3 is a constitutive hepatic steroid hormone 7 alpha -hydroxylase relatively unresponsive to a number of monooxygenase-inducing agents. The present study demonstrates that a polyhalogenated aromatic hydrocarbon inducer, 3,4,5,3',4',5'-hexachlorobiphenyl (HCB), induces P-450 3 in livers of adult male rats, and the increase is the result of an increase in the mRNA for this enzyme. Cytochrome P-450 3 and its mRNA were increased more slowly than cytochrome P-450c and its mRNA, indicating these enzymes are not regulated coordinately in liver. The maximum increase in P-450 3 and P-450 3-dependent androstenedione 7 alpha -hydroxylase activity (2- to 3-fold) occurred 7 days after administration of HCB. JF - Molecular Pharmacology AU - Yeowell, H N AU - Waxman, D J AU - LeBlanc, G A AU - Linko, P AU - Goldstein, JA AD - (B3-02), NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 272 EP - 278 VL - 33 IS - 3 SN - 0026-895X, 0026-895X KW - induction KW - 3,4,5,3',4',5'-hexachlorobiphenyl KW - cytochrome P450 KW - rats KW - Toxicology Abstracts KW - liver KW - X 24190:Polycyclic hydrocarbons UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15029080?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Pharmacology&rft.atitle=Induction+of+rat+cytochrome+P-450+3+and+its+mRNA+by+3%2C4%2C5%2C3%27%2C4%27%2C5%27-hexachlorobiphenyl.&rft.au=Yeowell%2C+H+N%3BWaxman%2C+D+J%3BLeBlanc%2C+G+A%3BLinko%2C+P%3BGoldstein%2C+JA&rft.aulast=Yeowell&rft.aufirst=H&rft.date=1988-01-01&rft.volume=33&rft.issue=3&rft.spage=272&rft.isbn=&rft.btitle=&rft.title=Molecular+Pharmacology&rft.issn=0026895X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - liver ER - TY - JOUR T1 - Model-building study of the combining sites of two antibodies to alpha (1 arrow right 6)dextran. AN - 15027255; 1838015 AB - Models of the Fv portion (containing the variable regions of the heavy and light chains) of two monoclonal anti- alpha (1 arrow right 6)dextran antibodies, W3129 and 19.1.2, were constructed from amino acid sequences and the known three-dimensional structures of the Fv portions of McPC603 and J539. The modeled combining site of W3129 has a protrusion on one side, formed by the long complementarity-determining region 1 of the light chain and the long complementarity-determining region 3 of the heavy chain, and has a cavity accommodating a glucose moiety. The model of the 19.1.2 site is basically flat with a shallow groove that can accommodate several internal glucose units. These results support the earlier conclusions, from ligand binding data, that W3129 has a cavity-type site, involving the terminal nonreducing glucose residue (endbinder), whereas 19.1.2 has a groove-type. JF - Proceedings of the National Academy of Sciences, USA AU - Padlan, E A AU - Kabat, E A AD - Lab. Mol. Biol., NIDDK, Natl. Inst. Health, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 6885 EP - 6889 VL - 85 IS - 18 SN - 0027-8424, 0027-8424 KW - amino acid sequence KW - antibodies KW - binding sites KW - structural model KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - F 06074:Antigen-antibody interactions UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15027255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Model-building+study+of+the+combining+sites+of+two+antibodies+to+alpha+%281+arrow+right+6%29dextran.&rft.au=Padlan%2C+E+A%3BKabat%2C+E+A&rft.aulast=Padlan&rft.aufirst=E&rft.date=1988-01-01&rft.volume=85&rft.issue=18&rft.spage=6885&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - amino acid sequence; antibodies ER - TY - JOUR T1 - Carbamate analogues of (-)-physostigmine: In vitro inhibition of acetyl- and butyrylcholinesterase. AN - 15005581; 1813722 AB - Reaction of (-)-eseroline with alkyl, aryl and aralkylisocyanates afforded a series of carbamate analogues of (-)-physostigmine which were assayed for inhibition of acetyl- and butyrylcholinesterase (AChE and BChE, respectively) in vitro. JF - FEBS Letters AU - Yu, Qian-Sheng AU - Atack, J R AU - Rapoport, ST AU - Brossi, A AD - Med. Chem. Sect., Lab. Anal. Chem., NIDDK, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 127 EP - 130 VL - 234 IS - 1 SN - 0014-5793, 0014-5793 KW - acetylcholinesterase KW - activity KW - analogs KW - butyrylcholinesterase KW - inhibition KW - physostigmine KW - Biochemistry Abstracts 3: Amino Acids, Peptides & Proteins (till 1993); Microbiology Abstracts B: Bacteriology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/15005581?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=Carbamate+analogues+of+%28-%29-physostigmine%3A+In+vitro+inhibition+of+acetyl-+and+butyrylcholinesterase.&rft.au=Yu%2C+Qian-Sheng%3BAtack%2C+J+R%3BRapoport%2C+ST%3BBrossi%2C+A&rft.aulast=Yu&rft.aufirst=Qian-Sheng&rft.date=1988-01-01&rft.volume=234&rft.issue=1&rft.spage=127&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 ER - TY - JOUR T1 - Demonstration of residual bone marrow effect in mice exposed to ethylene glycol monomethyl ether. AN - 14998570; 1808243 AB - Ethylene glycol monomethyl ether (EGMME) has been reported to cause hematopoietic abnormalities in man. The authors have shown that mice exposed to EGMME post-natally have suppressed bone marrow cellularity and progenitor cells 8 weeks post-exposure which returns to normal values by 16 weeks. Studies were designed to determine whether EGMME exposed mice that recovered had evidence of residual marrow stem cell injury. The results indicate that EGMME can cause persistent residual damage of bone marrow progenitor cells in mice, an effect that would not be apparent with routine hematological techniques. JF - Toxicology AU - Hong, H L AU - Silver, M AU - Boorman, G A AD - NIEHS (MD C2-02), P.O. Box 12233, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 107 EP - 115 VL - 50 IS - 1 SN - 0300-483X, 0300-483X KW - effects on KW - methyl cellosolve KW - hematopoiesis KW - hematopoietic pathology KW - mice KW - Health & Safety Science Abstracts; Pollution Abstracts; Toxicology Abstracts KW - toxicology KW - bone marrow KW - H SE4.20:POISONS AND POISONING KW - P 6000:TOXICOLOGY AND HEALTH KW - X 24152:Chronic exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14998570?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=Demonstration+of+residual+bone+marrow+effect+in+mice+exposed+to+ethylene+glycol+monomethyl+ether.&rft.au=Hong%2C+H+L%3BSilver%2C+M%3BBoorman%2C+G+A&rft.aulast=Hong&rft.aufirst=H&rft.date=1988-01-01&rft.volume=50&rft.issue=1&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - bone marrow; mice; toxicology ER - TY - JOUR T1 - The application of immunoassays and fluorometry to the detection of polycyclic hydrocarbon-macromolecular adducts and anti-adduct antibodies in humans. AN - 14966523; 1793824 AB - The metabolic activation of polycyclic aromatic hydrocarbons (PAH) to chemical species that form covalent adducts with cellular macromolecules (DNA and protein) is central to theories of carcinogenesis. Assays are currently being developed that will accurately reflect human macromolecular exposure to these carcinogens. Immunoassays are capable of detecting low levels of PAH-DNA adducts and antibodies directed against these adducts in humans and HPLC/spectrophotofluorimetry allows the detection of carcinogen-DNA or carcinogen-protein adducts in human peripheral blood. Both types of method have inherent advantages and disadvantages, and the use of more than one type of corroborative assay is a feature in this work. JF - International Archives of Occupational and Environmental Health AU - Weston, A AU - Rowe, M AU - Poirier, M AU - Trivers, G AU - Vahakangas, K AU - Newman, M AU - Haugen, A AU - Manchester, D AU - Mann, D AU - Harris, C AD - Lab. Human Carcinog., Div. Cancer Etiol., NCI, Bethesda, MD, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 157 EP - 162 VL - 60 IS - 3 SN - 0340-0131, 0340-0131 KW - pollutant detection KW - radioimmunoassay KW - Health & Safety Science Abstracts; Pollution Abstracts KW - occupational health KW - carcinogenesis KW - polycyclic aromatic hydrocarbons KW - H SI0.8.2:CHEMICALS (CORROSION) KW - P 6000:TOXICOLOGY AND HEALTH UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14966523?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Archives+of+Occupational+and+Environmental+Health&rft.atitle=The+application+of+immunoassays+and+fluorometry+to+the+detection+of+polycyclic+hydrocarbon-macromolecular+adducts+and+anti-adduct+antibodies+in+humans.&rft.au=Weston%2C+A%3BRowe%2C+M%3BPoirier%2C+M%3BTrivers%2C+G%3BVahakangas%2C+K%3BNewman%2C+M%3BHaugen%2C+A%3BManchester%2C+D%3BMann%2C+D%3BHarris%2C+C&rft.aulast=Weston&rft.aufirst=A&rft.date=1988-01-01&rft.volume=60&rft.issue=3&rft.spage=157&rft.isbn=&rft.btitle=&rft.title=International+Archives+of+Occupational+and+Environmental+Health&rft.issn=03400131&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - polycyclic aromatic hydrocarbons; carcinogenesis; occupational health ER - TY - JOUR T1 - Molecular characterization of gag proteins from simian immunodeficiency virus (SIV sub(Mne)). AN - 14960484; 1780802 AB - The authors report the results of amino acid sequence analysis of several purified proteins from SIV sub(Mne) and also homologous proteins from SIV sub(Mac). These protein sequences are readily aligned with each other and with the predicted amino acid sequences of proteins from SIV sub(Mac) and HIV-2. The results show the genomic origin of the SIV proteins and also reveal some of the proteolytic processing that takes place during viral maturation and thus help to better define the substrate specificities of the viral proteases. In addition, these data can readily be used to predict some cleavage sites in HIV-2 precursor polyproteins. JF - Journal of Virology AU - Henderson, LE AU - Benveniste, R E AU - Sowder, R AU - Copeland, T D AU - Schultz, A M AU - Oroszlan, S AD - Lab. Mol. Virol. and Carcinog., Bionetics Research, Inc., Basic Res. Program, NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 2587 EP - 2595 VL - 62 IS - 8 SN - 0022-538X, 0022-538X KW - amino acid sequence KW - comparison KW - gag gene KW - gene products KW - predictions KW - purification KW - simian immunodeficiency virus KW - structural proteins KW - Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - V 22002:AIDS: Molecular and in vitro aspects KW - V 22032:Viral proteins KW - J:20320 UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14960484?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Molecular+characterization+of+gag+proteins+from+simian+immunodeficiency+virus+%28SIV+sub%28Mne%29%29.&rft.au=Henderson%2C+LE%3BBenveniste%2C+R+E%3BSowder%2C+R%3BCopeland%2C+T+D%3BSchultz%2C+A+M%3BOroszlan%2C+S&rft.aulast=Henderson&rft.aufirst=LE&rft.date=1988-01-01&rft.volume=62&rft.issue=8&rft.spage=2587&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - structural proteins; amino acid sequence; gene products ER - TY - JOUR T1 - The effects of mono-(2-ethylhexyl)-phthalate on rat sertoli cell-enriched primary cultures. AN - 14947116; 1776604 AB - There is evidence from in vivo studies that the Sertoli cell is an initial target cell for the actions of phthalates in the rodent testis. Because this metabolically active cell type plays a central role in spermatogenesis, the authors examined the effects of a toxic phthalate, mono-(2-ethylhexyl)-phthalate (MEHP), on the secretory and synthetic activities of primary testicular cell cultures from 18-day-old rats. These cultures were 78-84% Sertoli cells. Exposure to MEHP decreased cellular ATP by 20%, decreased production of radiolabeled super(14)CO sub(2) from acetate, and decreased media levels of pyruvate, while it increased media levels of lactate and intracellular lipid. Protein synthesis, was not affected by MEHP. Mitochondrial succinate dehydrogenase activity was decreased. Michaelis-Menton kinetic analysis indicated this was a mixed inhibition. There was no change in mitochondrial Rhodamine 123 uptake. JF - Toxicology and Applied Pharmacology AU - Chapin, R E AU - Gray, TJB AU - Phelps, J L AU - Dutton, S L AD - NIEHS, P.O. Box 12233, Mail Drop E2-01, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 467 EP - 479 VL - 92 IS - 3 SN - 0041-008X, 0041-008X KW - effects on KW - mono-(2-ethylhexyl)-phthalate KW - rats KW - Toxicology Abstracts KW - Sertoli cells KW - X 24151:Acute exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14947116?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=The+effects+of+mono-%282-ethylhexyl%29-phthalate+on+rat+sertoli+cell-enriched+primary+cultures.&rft.au=Chapin%2C+R+E%3BGray%2C+TJB%3BPhelps%2C+J+L%3BDutton%2C+S+L&rft.aulast=Chapin&rft.aufirst=R&rft.date=1988-01-01&rft.volume=92&rft.issue=3&rft.spage=467&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - Sertoli cells ER - TY - JOUR T1 - Alcohols stimulate gamma -aminobutyric acid receptor-mediated chloride uptake in brain vesicles: Correlation with intoxication potency. AN - 14929701; 1765210 AB - A series of short-chain alcohols, including ethanol, were examined for their abilities to stimulate gamma -aminobutyric acid (GABA) receptor-mediated chloride uptake into isolated brain vesicles. All of the alcohols tested stimulated super(36)chloride uptake, at concentrations that occur during acute intoxication, and their potencies in stimulating GABA receptor-mediated chloride uptake were highly correlated with both their intoxication potencies in rats and their membrane/buffer partition coefficients. Thus, the activity of alcohols at the GABA receptor-coupled chloride ion channel appears to be related to their ability to enter hydrophobic regions of the neuronal membrane. The data suggest that the anxiolytic, sedative/hypnotic and intoxicating properties of ethanol may, in part, be mediated via an action at central GABA receptors. JF - Brain Research AU - Suzdak, P D AU - Schwartz, R D AU - Skolnick, P AU - Paul, S M AD - Sect. Mol. Pharmacol., Clin. Neurosci. Branch, NIMH, Build. 10, Rm. 4N214, 9000 Rockville Pike, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 340 EP - 345 VL - 444 IS - 2 SN - 0006-8993, 0006-8993 KW - mediation KW - uptake KW - stimulation KW - gamma -aminobutyric acid KW - chloride KW - alcohols KW - brain KW - membrane vesicles KW - Toxicology Abstracts; Biochemistry Abstracts 1: Biological Membranes (till 1993); CSA Neurosciences Abstracts KW - N3 11094:Central nervous system KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14929701?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+Research&rft.atitle=Alcohols+stimulate+gamma+-aminobutyric+acid+receptor-mediated+chloride+uptake+in+brain+vesicles%3A+Correlation+with+intoxication+potency.&rft.au=Suzdak%2C+P+D%3BSchwartz%2C+R+D%3BSkolnick%2C+P%3BPaul%2C+S+M&rft.aulast=Suzdak&rft.aufirst=P&rft.date=1988-01-01&rft.volume=444&rft.issue=2&rft.spage=340&rft.isbn=&rft.btitle=&rft.title=Brain+Research&rft.issn=00068993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - membrane vesicles; brain ER - TY - JOUR T1 - Reproductive toxicity of tricresyl phosphate in a continuous breeding protocol in Swiss (CD-1) mice. AN - 14927762; 1753757 AB - The effects of a mixture of tricresyl phosphate isomers on reproductive performance in Swiss (CD-1) mice were evaluated using a continuous breeding protocol. Tricresyl phosphate (TCP) was mixed into the feed at 0, 0.05, 0.1, and 0.2% by weight. Although the fertility index was not changed in the animals consuming the high-concentration feed, the number of litters per pair decreased in a dose-related fashion, and the proportion of pups born alive, and their weight, was significantly decreased in the high-dose group. A crossover mating trial found impaired fertility in both males and females exposed to 0.2% TCP, with a greater effect in females. Histopathology of the F sub(0) pairs revealed dose-related seminiferous tubule atrophy, and decreased testis and epididymal weights in the high-dose males, while the female reproductive tract showed no histopathologic changes. There were dose-related changes in the adrenals of both sexes, and body weight was depressed in both sexes at the highest concentration. JF - Fundamental and Applied Toxicology AU - Chapin, R E AU - George, J D AU - Lamb, JC IV AD - NIEHS, P.O. Box 12233, Mail Drop E2-01, Research Triangle Park, NC 27709, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 344 EP - 354 VL - 10 IS - 2 SN - 0272-0590, 0272-0590 KW - effects on KW - tritolyl phosphate KW - mice KW - Toxicology Abstracts KW - reproduction KW - teratogenicity KW - X 24152:Chronic exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14927762?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Fundamental+and+Applied+Toxicology&rft.atitle=Reproductive+toxicity+of+tricresyl+phosphate+in+a+continuous+breeding+protocol+in+Swiss+%28CD-1%29+mice.&rft.au=Chapin%2C+R+E%3BGeorge%2C+J+D%3BLamb%2C+JC+IV&rft.aulast=Chapin&rft.aufirst=R&rft.date=1988-01-01&rft.volume=10&rft.issue=2&rft.spage=344&rft.isbn=&rft.btitle=&rft.title=Fundamental+and+Applied+Toxicology&rft.issn=02720590&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - reproduction; teratogenicity ER - TY - JOUR T1 - The sequence of porcine chromogranin A messenger RNA demonstrates chromogranin A can serve as the precursor for the biologically active hormone, pancreastatin. AN - 14902227; 1754090 AB - Specific oligonucleotide priming of double-stranded DNA has been employed to sequence a porcine chromogranin A adrenomedullary cDNA. Porcine chromogranin A can serve as the precursor for pancreastatin, a polypeptide capable of inhibiting insulin release from the endocrine pancreas and acid secretion from parietal cells of the gut. JF - Endocrinology AU - Iacangelo, AL AU - Fischer-Colbrie, R AU - Koller, K J AU - Brownstein, MJ AU - Eiden, LE AD - Unit. Mol. and Cell. Neurobiol., NIMH, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 2339 EP - 2341 VL - 122 IS - 5 SN - 0013-7227, 0013-7227 KW - amino acid sequence KW - chromogranin A KW - pancreastatin KW - pigs KW - precursors KW - Microbiology Abstracts B: Bacteriology; CSA Neurosciences Abstracts KW - J:20320 KW - N3 11370:GASTRIN AND OTHER GASTROINTESTINAL PEPTIDES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14902227?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Endocrinology&rft.atitle=The+sequence+of+porcine+chromogranin+A+messenger+RNA+demonstrates+chromogranin+A+can+serve+as+the+precursor+for+the+biologically+active+hormone%2C+pancreastatin.&rft.au=Iacangelo%2C+AL%3BFischer-Colbrie%2C+R%3BKoller%2C+K+J%3BBrownstein%2C+MJ%3BEiden%2C+LE&rft.aulast=Iacangelo&rft.aufirst=AL&rft.date=1988-01-01&rft.volume=122&rft.issue=5&rft.spage=2339&rft.isbn=&rft.btitle=&rft.title=Endocrinology&rft.issn=00137227&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - amino acid sequence ER - TY - JOUR T1 - Microinjection and expression of an infectious proviral clone and subgenomic envelope construct of a human immunodeficiency virus. AN - 14876781; 1716223 AB - An infectious proviral clone of the human immunodeficiency virus (HIV) was microinjected into the cell nucleus in six cell lines derived from caprine, ovine, bovine, or human solid tissue to study the utility of this method in effecting viral gene expression in nonlymphoid cells. Immunofluorescence assays for HIV demonstrated viral gene expression in only 5% of cells 24-48 h after microinjection; however, no reverse transcriptase activity was detectable, presumably due to a low level of virus release in this limited number of cell. To indirectly assess infectious virus releases, microinjected cells were cocultured with human T4 antigen-positive lymphocytes (H9) sensitive to HIV infection. Syncytia formation, electron microscopy, reverse transcriptase activity, and radioimmunoassay for HIV p24 were used to monitor viral gene expression in cocultures. HIV was efficiently recovered by cocultivating H9 with microinjected cells 48 h after microinjection, regardless of the tissue type or species of origin. JF - AIDS Research and Human Retroviruses AU - Boyd, AL AU - Wood, T G AU - Buckley, A AU - Fischinger, P J AU - Gilden, R V AU - Gonda, MA AD - Lab. Cell and Mol. Struct., Program Resour., Inc., NCI-Frederick Cancer Res. Facil., Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 31 EP - 41 VL - 4 IS - 1 SN - 0889-2229, 0889-2229 KW - man KW - envelopes KW - gene expression KW - genes KW - helper cells KW - human immunodeficiency virus KW - microinjection KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Virology & AIDS Abstracts KW - V 22050:Viral genetics including virus reactivation KW - V 22002:AIDS: Molecular and in vitro aspects KW - G 07120:Recombinant DNA/Genetic engineering KW - W 30129:Others KW - N 14676:Microinjection UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14876781?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=AIDS+Research+and+Human+Retroviruses&rft.atitle=Microinjection+and+expression+of+an+infectious+proviral+clone+and+subgenomic+envelope+construct+of+a+human+immunodeficiency+virus.&rft.au=Boyd%2C+AL%3BWood%2C+T+G%3BBuckley%2C+A%3BFischinger%2C+P+J%3BGilden%2C+R+V%3BGonda%2C+MA&rft.aulast=Boyd&rft.aufirst=AL&rft.date=1988-01-01&rft.volume=4&rft.issue=1&rft.spage=31&rft.isbn=&rft.btitle=&rft.title=AIDS+Research+and+Human+Retroviruses&rft.issn=08892229&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - human immunodeficiency virus; envelopes; genes; microinjection; helper cells; gene expression ER - TY - JOUR T1 - Morphological and neurochemical effects of diazepam and phenobarbital on selective culture of neurons from fetal rat brain. AN - 14864009; 1712864 AB - The responses to diazepam (DZ) and phenobarbital (PhB) were studied in enriched neuronal primary cultures from rat embryo hemispheres. It was concluded that both DZ and PhB might affect, at least transiently, the normal growth of neurons in culture. JF - Journal of Neurochemistry AU - Daval, J-L AU - De Vasconcelos, AP AU - Lartaud, I AD - Unit Neurochem., BPB, NIMH, Build. 10, Rm. 3C-210, Bethesda, MD 20892, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 665 EP - 672 VL - 50 IS - 3 SN - 0022-3042, 0022-3042 KW - disruption KW - in vitro KW - diazepam KW - phenobarbital KW - rats KW - Toxicology Abstracts; CSA Neurosciences Abstracts KW - neurons KW - brain KW - growth KW - N3 11104:Mammals (except primates) KW - X 24117:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14864009?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neurochemistry&rft.atitle=Morphological+and+neurochemical+effects+of+diazepam+and+phenobarbital+on+selective+culture+of+neurons+from+fetal+rat+brain.&rft.au=Daval%2C+J-L%3BDe+Vasconcelos%2C+AP%3BLartaud%2C+I&rft.aulast=Daval&rft.aufirst=J-L&rft.date=1988-01-01&rft.volume=50&rft.issue=3&rft.spage=665&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - growth; neurons; brain ER - TY - JOUR T1 - The 4-6-8 method of sequence analysis. AN - 14832417; 1693645 AB - A new method to obtain more sequence data from a single gel run is described. This method allows the reading of over 500 bases of sequence data from a single gel by taking advantage of the differential migration of specific sized dideoxy terminated chain lengths in sequencing gels containing variable percentages of acrylamide. JF - Biotechniques AU - Isfort, R J AU - Ihle, J N AD - NCI - Frederick Cancer Res. Facil., BRI - Basic Res. Program, P.O. Box B, Frederick, MD 21701, USA Y1 - 1988 PY - 1988 DA - 1988 SP - 138 EP - 131 VL - 6 IS - 2 SN - 0736-6205, 0736-6205 KW - determination KW - methodology KW - 4-6-8 method KW - DNA KW - nucleotide sequence KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - N 14640:Structure & sequence KW - W 30119:Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/14832417?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biotechniques&rft.atitle=The+4-6-8+method+of+sequence+analysis.&rft.au=Isfort%2C+R+J%3BIhle%2C+J+N&rft.aulast=Isfort&rft.aufirst=R&rft.date=1988-01-01&rft.volume=6&rft.issue=2&rft.spage=138&rft.isbn=&rft.btitle=&rft.title=Biotechniques&rft.issn=07366205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-12 N1 - SubjectsTermNotLitGenreText - DNA; nucleotide sequence ER - TY - JOUR T1 - Waterborne non-A, non-B hepatitis AN - 13788759; 198802266 AB - The incidence of waterborne non-A, non-B hepatitis had been surveyed. The disease was usually mild, except in pregnant women, among whom there was a high fatality rate. Faecal contamination of drinking water was normally implicated in outbreaks of the disease. Hepatitis A and B virus infection could be serologically excluded, and liver biopsy specimens had characteristic histological features. The disease was widespread in India, South-East Asia, and Africa and might occur in Latin America. Clean water supplies and safer excreta disposal were the main control measures. JF - Lancet AU - Ramalingaswami, V AU - Purcell, R H AD - National Institutes of Health, Bethesda, Md. Y1 - 1988 PY - 1988 DA - 1988 SP - 571 EP - 573 IS - 8585 SN - 0099-5355, 0099-5355 KW - Diseases (see also individual groups below) KW - Waterborne KW - Viruses (-general-) (see also individ grps below) KW - Aqualine Abstracts KW - AQ 00002:Water Quality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/13788759?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aaqualine&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lancet&rft.atitle=Waterborne+non-A%2C+non-B+hepatitis&rft.au=Ramalingaswami%2C+V%3BPurcell%2C+R+H&rft.aulast=Ramalingaswami&rft.aufirst=V&rft.date=1988-01-01&rft.volume=&rft.issue=8585&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Lancet&rft.issn=00995355&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2000-09-01 N1 - SuppNotes - Publication focus: General. N1 - Last updated - 2011-12-12 ER - TY - JOUR T1 - Ultrastructural and biochemical studies of intracellular metal-binding patterns in kidney tubule cells of the scallop Placopecten magellanicus following prolonged exposure to cadmium or copper AN - 13766849; S198929770 AB - Scallops in early gametogenesis were exposed to cadmium or copper (20 ug per litre) for 7 weeks. Cadmium had no effect on kidney ultrastructure, but kidneys from copper exposed scallops contained few concentrations and showed marked signs of cellular degeneration characterized by loss of membrane integrity. Ultrastructural changes were associated with reductions in renal isocitrate dehydrogenase activity. Analysis of kidney concentration fractions from cadmium treated scallops showed a 6-fold increase in cadmium concentrations of copper, zinc and manganese, respectively, compared with controls. Analysis of kidney cytosolic metal-binding protein fractions showed 7-fold and 5-fold increases in cadmium and zinc and 50 per cent reductions in copper treated scallops, there was a 5-fold increase in copper and 99, 95 and 76 per cent reductions in cadmium, zinc and manganese. These findings supported the hypothesis that metal induced disruption of the normal homeostatic mechanisms controlling divalent metal cation bioavailability may be an important factor in mediating cell injury. JF - Marine Biology AU - Fowler, BA AU - Gould, E AD - National Institute of Environmental Health Sciences, Research Triangle Park, N.C. Y1 - 1988 PY - 1988 DA - 1988 SP - 207 EP - 216 VL - 97 IS - 2 SN - 0025-3162, 0025-3162 KW - Homeostatically KW - Tubule KW - Aqualine Abstracts KW - AQ 00008:Effects of Pollution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/13766849?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aaqualine&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Marine+Biology&rft.atitle=Ultrastructural+and+biochemical+studies+of+intracellular+metal-binding+patterns+in+kidney+tubule+cells+of+the+scallop+Placopecten+magellanicus+following+prolonged+exposure+to+cadmium+or+copper&rft.au=Fowler%2C+BA%3BGould%2C+E&rft.aulast=Fowler&rft.aufirst=BA&rft.date=1988-01-01&rft.volume=97&rft.issue=2&rft.spage=207&rft.isbn=&rft.btitle=&rft.title=Marine+Biology&rft.issn=00253162&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2000-09-01 N1 - SuppNotes - Publication focus: Experimental. N1 - Last updated - 2011-12-12 ER - TY - JOUR T1 - Tetanus toxin binds with high affinity to neuroblastoma x glioma hybrid cells NG 108-15 and impairs their stimulated acetylcholine release. AN - 77883033; 2826418 AB - Differentiated neuroblastoma x glioma hybrid cells NG 108-15 express on their surface specific binding sites for tetanus toxin. 450 sites/cell with a KD of 2 x 10(-11) M were found under "physiological" conditions of pH and salt concentrations. A Hill coefficient of 1.1 indicated noncooperative binding. Specific binding of 125I-toxin to its sites could be prevented either by preincubation of the toxin with a neutralizing monoclonal antibody or by pretreatment of the cells with neuraminidase (Vibrio cholerae). To quantify the action of tetanus toxin on the stimulated release of 14C activity from differentiated cells preincubated with [14C]choline, a new type of perfusion device was designed which could be filled with cells growing in monolayers on Cytodex-3 microbeads. Tetanus toxin inhibited the stimulated 14C release in a time- and dose-dependent manner. A greater than 50% inhibition was found after 2 h of incubation with 10(-12) M toxin. The inhibitory action of tetanus toxin could be prevented with a monoclonal antibody to the toxin or with neuraminidase treatment of the cells. These results suggest that the neuraminidase-sensitive 2 x 10(-11) KD receptors are the productive receptors for tetanus intoxication in differentiated NG 108-15 cells. The possible chemical composition of these receptors is discussed. Differentiated NG 108-15 cells provide a useful model in which picomolar tetanus concentrations produce both measurable saturable binding and inhibition of potassium-evoked, acetylcholine release under physiological conditions of pH and salt concentrations. JF - The Journal of biological chemistry AU - Wellhöner, H H AU - Neville, D M AD - Section on Biophysical Chemistry, National Institute of Mental Health, Bethesda, Maryland 20892. Y1 - 1987/12/25/ PY - 1987 DA - 1987 Dec 25 SP - 17374 EP - 17378 VL - 262 IS - 36 SN - 0021-9258, 0021-9258 KW - Tetanus Toxin KW - 0 KW - Bucladesine KW - 63X7MBT2LQ KW - Neuraminidase KW - EC 3.2.1.18 KW - Acetylcholine KW - N9YNS0M02X KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Neuraminidase -- metabolism KW - Glioma -- metabolism KW - Bucladesine -- pharmacology KW - Time Factors KW - Neuroblastoma -- metabolism KW - Hybridomas -- metabolism KW - Acetylcholine -- metabolism KW - Tumor Cells, Cultured -- metabolism KW - Tetanus Toxin -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77883033?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Tetanus+toxin+binds+with+high+affinity+to+neuroblastoma+x+glioma+hybrid+cells+NG+108-15+and+impairs+their+stimulated+acetylcholine+release.&rft.au=Wellh%C3%B6ner%2C+H+H%3BNeville%2C+D+M&rft.aulast=Wellh%C3%B6ner&rft.aufirst=H&rft.date=1987-12-25&rft.volume=262&rft.issue=36&rft.spage=17374&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-29 N1 - Date created - 1988-01-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alternative internal splicing in c-myb RNAs occurs commonly in normal and tumor cells. AN - 77974464; 2832149 AB - Activation of the c-myb gene by viral transduction or proviral insertional mutagenesis that is likely to result in the production of structurally altered myb proteins has been shown to be predominantly associated with myelomonocytic tumors. An alternative splicing event in which a portion of the intron bounded by the vE6 and vE7 exons with v-myb homology is included as an additional 363-nucleotide coding exon has recently been identified in a mouse tumor that carries a provirus-activated myb gene. This alternative splicing was hypothesized to be a tumor-specific aberrant form of 3'-myb RNA processing as a consequence of the disruption of upstream 5'-sequences by proviral insertion. However, RNA blot analyses and RNase mapping studies presented here show that a significant portion (approximately 10%) of all myb transcripts examined, whether in normal or in clonal tumor cells, contains the additional exon. Hence the alternative splicing is a hitherto unrecognized common normal event that potentially increases the diversity of the myb proteins expressed in normal tissues including thymus and spleen, as well as in tumor cells with either normal or 5'-rearranged myb alleles. The lack of change in the ratio of the two spliced products expressed from either the normal or the 5'-rearranged myb further indicates that the insertion of the unique 121 amino acids in the larger myb transcripts is not a consequence of tumor-specific activation of the mouse myb oncogene. JF - The EMBO journal AU - Shen-Ong, G L AD - Laboratory of Genetics, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12/20/ PY - 1987 DA - 1987 Dec 20 SP - 4035 EP - 4039 VL - 6 IS - 13 SN - 0261-4189, 0261-4189 KW - RNA, Neoplasm KW - 0 KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Index Medicus KW - Animals KW - Reference Values KW - Organ Specificity KW - Mice KW - Gene Expression Regulation KW - Mice, Inbred BALB C KW - Leukemia, Experimental -- genetics KW - Cell Line KW - Cloning, Molecular KW - RNA Splicing KW - Transcription, Genetic KW - RNA, Neoplasm -- genetics KW - Proto-Oncogenes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77974464?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+EMBO+journal&rft.atitle=Alternative+internal+splicing+in+c-myb+RNAs+occurs+commonly+in+normal+and+tumor+cells.&rft.au=Shen-Ong%2C+G+L&rft.aulast=Shen-Ong&rft.aufirst=G&rft.date=1987-12-20&rft.volume=6&rft.issue=13&rft.spage=4035&rft.isbn=&rft.btitle=&rft.title=The+EMBO+journal&rft.issn=02614189&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-05-04 N1 - Date created - 1988-05-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Cell Biol. 1982 Jun;2(6):617-24 [14582157] Genes Dev. 1987 May;1(3):287-96 [2445628] Science. 1982 Jun 25;216(4553):1421-3 [6283631] Proc Natl Acad Sci U S A. 1982 Apr;79(7):2194-8 [6954533] Cell. 1982 Dec;31(2 Pt 1):453-63 [6297766] Science. 1983 May 20;220(4599):795-8 [6687762] Nature. 1983 Nov 24-30;306(5941):391-5 [6316155] Nature. 1983 Nov 24-30;306(5941):395-7 [6316156] Science. 1984 Nov 30;226(4678):1077-80 [6093260] Proc Natl Acad Sci U S A. 1985 Oct;82(19):6687-91 [2995979] EMBO J. 1985 Aug;4(8):2003-8 [2998780] Proc Natl Acad Sci U S A. 1986 May;83(10):3204-8 [3010282] Proc Natl Acad Sci U S A. 1986 Jul;83(13):4685-9 [3088565] Annu Rev Biochem. 1986;55:1091-117 [3017190] Annu Rev Biochem. 1986;55:1119-50 [2943217] Mol Cell Biol. 1986 Feb;6(2):380-92 [3023843] Proc Natl Acad Sci U S A. 1987 Jan;84(1):199-203 [3025854] Cell. 1987 Jan 30;48(2):177-8 [2948653] Annu Rev Genet. 1986;20:361-84 [3028245] Proc Natl Acad Sci U S A. 1987 May;84(10):3171-5 [3033638] J Virol. 1987 Jul;61(7):2339-43 [2884332] Science. 1987 Jul 24;237(4813):411-5 [2440106] EMBO J. 1987 Jun;6(6):1643-51 [3608990] EMBO J. 1987 Jul;6(7):2027-35 [2958276] EMBO J. 1987 Jul;6(7):2037-44 [2958277] Science. 1973 Nov 9;182(4112):592-4 [4355680] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Modulation by palmitoylcarnitine of protein kinase C activation. AN - 81108580; 3479247 AB - Palmitoylcarnitine, a reported protein kinase C inhibitor, enhanced the phorbol ester dependency of the enzyme, augmenting protein kinase C activity in the presence of phorbol esters such as phorbol 12,13-dibutyrate while inhibiting the basal activity measured in the presence of calcium plus phosphatidylserine. Weakly potent phorbol esters such as phorbol 12,13-diacetate and 4-O-methylphorbol 12-myristate 13-acetate were full agonists like phorbol 12,13-dibutyrate for activation of protein kinase C in the presence of palmitoylcarnitine. On the other hand, 1,2-diacylglycerols such as 1,2-diolein were only partially stimulatory. Palmitoylcarnitine did not interfere with the association of protein kinase C with phosphatidylserine, suggesting that its action was on protein kinase C activation per se rather than on priming. A long fatty acid ester, quaternary amine, and anionic charge were needed for the palmitoylcarnitine-like action. Phosphatidylcholine, which possesses these features, partially mimicked the action of palmitoylcarnitine. Palmitoylcarnitine thus appears to be a lipophilic modulator of protein kinase C rather than a simple inhibitor. The results raise the possibility that differences in response between phorbol esters and diacylglycerols may reflect differential ability to activate protein kinase C in the appropriate lipid environment rather than the existence of unique targets for one or the other compound. JF - Cancer research AU - Nakadate, T AU - Blumberg, P M AD - Molecular Mechanisms of Tumor Promotion Section, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12/15/ PY - 1987 DA - 1987 Dec 15 SP - 6537 EP - 6542 VL - 47 IS - 24 Pt 1 SN - 0008-5472, 0008-5472 KW - Diglycerides KW - 0 KW - Histones KW - Phorbol Esters KW - Phospholipids KW - Palmitoylcarnitine KW - 1935-18-8 KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Carnitine KW - S7UI8SM58A KW - Calcium KW - SY7Q814VUP KW - diolein KW - Z3MP1W91CW KW - Index Medicus KW - Calcium -- metabolism KW - Phorbol Esters -- pharmacology KW - Animals KW - Diglycerides -- pharmacology KW - Histones -- metabolism KW - Phospholipids -- metabolism KW - Enzyme Activation -- drug effects KW - Mice KW - Protein Kinase C -- metabolism KW - Palmitoylcarnitine -- pharmacology KW - Carnitine -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81108580?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Modulation+by+palmitoylcarnitine+of+protein+kinase+C+activation.&rft.au=Nakadate%2C+T%3BBlumberg%2C+P+M&rft.aulast=Nakadate&rft.aufirst=T&rft.date=1987-12-15&rft.volume=47&rft.issue=24+Pt+1&rft.spage=6537&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-21 N1 - Date created - 1988-01-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Heterogeneous DNA damage and repair in the mammalian genome. AN - 81106274; 3315187 JF - Cancer research AU - Bohr, V A AU - Phillips, D H AU - Hanawalt, P C AD - Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12/15/ PY - 1987 DA - 1987 Dec 15 SP - 6426 EP - 6436 VL - 47 IS - 24 Pt 1 SN - 0008-5472, 0008-5472 KW - Alkylating Agents KW - 0 KW - Carcinogens KW - Chromatin KW - DNA, Mitochondrial KW - Tetrahydrofolate Dehydrogenase KW - EC 1.5.1.3 KW - Index Medicus KW - Carcinogens -- pharmacology KW - Animals KW - DNA, Mitochondrial -- drug effects KW - Oncogenes KW - Humans KW - Alkylating Agents -- pharmacology KW - Chromatin -- drug effects KW - Transcription, Genetic KW - Repetitive Sequences, Nucleic Acid -- drug effects KW - Proto-Oncogenes KW - Nucleic Acid Conformation KW - Tetrahydrofolate Dehydrogenase -- genetics KW - Alkylation KW - DNA Repair KW - DNA Damage KW - Mammals -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81106274?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Heterogeneous+DNA+damage+and+repair+in+the+mammalian+genome.&rft.au=Bohr%2C+V+A%3BPhillips%2C+D+H%3BHanawalt%2C+P+C&rft.aulast=Bohr&rft.aufirst=V&rft.date=1987-12-15&rft.volume=47&rft.issue=24+Pt+1&rft.spage=6426&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-21 N1 - Date created - 1988-01-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: Cancer Res 1988 Mar 1;48(5):1377 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - A Taq I polymorphism in the human P450IIE1 gene on chromosome 10 (CYP2E). AN - 77895502; 2892165 JF - Nucleic acids research AU - McBride, O W AU - Umeno, M AU - Gelboin, H V AU - Gonzalez, F J AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12/10/ PY - 1987 DA - 1987 Dec 10 SP - 10071 VL - 15 IS - 23 SN - 0305-1048, 0305-1048 KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Deoxyribonucleases, Type II Site-Specific KW - EC 3.1.21.4 KW - TCGA-specific type II deoxyribonucleases KW - Index Medicus KW - Genes KW - Humans KW - Polymorphism, Restriction Fragment Length KW - Polymorphism, Genetic KW - Chromosomes, Human, Pair 10 KW - Chromosome Mapping UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77895502?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+research&rft.atitle=A+Taq+I+polymorphism+in+the+human+P450IIE1+gene+on+chromosome+10+%28CYP2E%29.&rft.au=McBride%2C+O+W%3BUmeno%2C+M%3BGelboin%2C+H+V%3BGonzalez%2C+F+J&rft.aulast=McBride&rft.aufirst=O&rft.date=1987-12-10&rft.volume=15&rft.issue=23&rft.spage=10071&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1986 Dec 15;261(35):16689-97 [3782137] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - cDNA and amino acid sequences of two members of the human P450IIC gene subfamily. AN - 77882360; 3697070 JF - Nucleic acids research AU - Kimura, S AU - Pastewka, J AU - Gelboin, H V AU - Gonzalez, F J AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12/10/ PY - 1987 DA - 1987 Dec 10 SP - 10053 EP - 10054 VL - 15 IS - 23 SN - 0305-1048, 0305-1048 KW - DNA KW - 9007-49-2 KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Index Medicus KW - Base Sequence KW - Humans KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Cloning, Molecular KW - DNA -- isolation & purification KW - Cytochrome P-450 Enzyme System -- genetics KW - Cytochrome P-450 Enzyme System -- classification UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77882360?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+research&rft.atitle=cDNA+and+amino+acid+sequences+of+two+members+of+the+human+P450IIC+gene+subfamily.&rft.au=Kimura%2C+S%3BPastewka%2C+J%3BGelboin%2C+H+V%3BGonzalez%2C+F+J&rft.aulast=Kimura&rft.aufirst=S&rft.date=1987-12-10&rft.volume=15&rft.issue=23&rft.spage=10053&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-23 N1 - Date created - 1988-02-23 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 [271968] Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 [6219389] Biochemistry. 1987 Feb 24;26(4):1094-9 [3032244] J Biol Chem. 1986 Aug 15;261(23):10667-72 [3015936] DNA. 1987 Feb;6(1):1-11 [3829886] Anal Biochem. 1983 Feb 15;129(1):216-23 [6305233] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Temporal separation of protein toxin translocation from processing events. AN - 77823647; 3680260 AB - Intoxication of Vero cells by ricin, modeccin, diphtheria toxin (DT), and Pseudomonas exotoxin A requires: 1) binding to cell surface receptors; 2) transport to the cytoplasm; and 3) enzymatic inactivation of a component of the protein synthetic machinery. The kinetic profiles of all four toxins consist of a lag followed by the apparent first-order decrease in protein synthesis. Autoradiographic analysis of DT-intoxicated cell populations has demonstrated that two subpopulations of cells exist during the period of decreasing protein synthesis: one population synthesizing at control levels and the other synthesizing little or no protein (Hudson, T. H., and Neville, D. M., Jr. (1985) J. Biol. Chem. 260, 2675-2680). The present study correlates the autoradiographic data with the rates of protein synthesis decline in cells intoxicated with modeccin, ricin, Pseudomonas exotoxin A, as well DT. In all cases, the first time point which exhibits a decrease in protein synthetic activity also exhibits two subpopulations of cells, one synthesizing protein at control rates and the other synthesizing little or no protein. As the intoxication progresses, cells leave the control population by the rapid cessation of all protein synthesis. These experiments demonstrate that transport of all four toxins to the cytosol is the rate-limiting step during the pseudo first-order decline in protein synthesis. Furthermore, the final step in the transport process (translocation) must result in the release to the cytoplasm of a quantity of toxin sufficient to rapidly inactivate all protein synthesis in that cell. The probability of a translocation event occurring in any cell of the population is established during the lag and remains constant throughout the first-order decrease in protein synthesis. The requirement for acidification during the intoxication by DT, Pseudomonas exotoxin A, or modeccin is restricted to the lag period. Acidification is therefore necessary to establish the probability of translocation, but it is not directly involved in the actual translocation of these toxins. The pseudo first-order passage of DT intoxications through antitoxin and NH4Cl- or monensin-sensitive stages are shown to have the same cellular basis as the pseudo first-order decrease in protein synthesis. A kinetic model is presented which defines the DT intoxication process from one of its earliest events (endocytosis) to its penultimate event (translocation of toxin to the cytosol).(ABSTRACT TRUNCATED AT 400 WORDS) JF - The Journal of biological chemistry AU - Hudson, T H AU - Neville, D M AD - Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892. Y1 - 1987/12/05/ PY - 1987 DA - 1987 Dec 05 SP - 16484 EP - 16494 VL - 262 IS - 34 SN - 0021-9258, 0021-9258 KW - Bacterial Toxins KW - 0 KW - Diphtheria Toxin KW - Exotoxins KW - Lectins KW - Plant Lectins KW - Ribosome Inactivating Proteins, Type 2 KW - Virulence Factors KW - Ammonium Chloride KW - 01Q9PC255D KW - modeccin KW - 65988-88-7 KW - Ricin KW - 9009-86-3 KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - toxA protein, Pseudomonas aeruginosa KW - EC 2.4.2.31 KW - Index Medicus KW - Cytosol -- metabolism KW - Animals KW - Kinetics KW - Hydrogen-Ion Concentration KW - Cercopithecus aethiops KW - Biological Transport, Active KW - Ammonium Chloride -- pharmacology KW - Time Factors KW - Cell Line KW - Lectins -- pharmacokinetics KW - Ricin -- pharmacokinetics KW - Diphtheria Toxin -- pharmacokinetics KW - Exotoxins -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77823647?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Temporal+separation+of+protein+toxin+translocation+from+processing+events.&rft.au=Hudson%2C+T+H%3BNeville%2C+D+M&rft.aulast=Hudson&rft.aufirst=T&rft.date=1987-12-05&rft.volume=262&rft.issue=34&rft.spage=16484&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-12 N1 - Date created - 1988-01-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. AN - 77843994; 3500513 AB - The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype. JF - Science (New York, N.Y.) AU - Velu, T J AU - Beguinot, L AU - Vass, W C AU - Willingham, M C AU - Merlino, G T AU - Pastan, I AU - Lowy, D R AD - Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12/04/ PY - 1987 DA - 1987 Dec 04 SP - 1408 EP - 1410 VL - 238 IS - 4832 SN - 0036-8075, 0036-8075 KW - DNA, Recombinant KW - 0 KW - Recombinant Proteins KW - Epidermal Growth Factor KW - 62229-50-9 KW - Receptor, Epidermal Growth Factor KW - EC 2.7.10.1 KW - Index Medicus KW - Animals KW - Fibroblasts -- pathology KW - Neoplasms, Experimental -- etiology KW - Cells, Cultured KW - Humans KW - Genetic Vectors KW - Harvey murine sarcoma virus -- genetics KW - Mice, Nude KW - Mice KW - Recombinant Proteins -- genetics KW - Male KW - Receptor, Epidermal Growth Factor -- drug effects KW - Receptor, Epidermal Growth Factor -- genetics KW - Cell Transformation, Neoplastic -- chemically induced KW - Epidermal Growth Factor -- pharmacology KW - Proto-Oncogenes KW - Cell Transformation, Neoplastic -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77843994?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Science+%28New+York%2C+N.Y.%29&rft.atitle=Epidermal-growth-factor-dependent+transformation+by+a+human+EGF+receptor+proto-oncogene.&rft.au=Velu%2C+T+J%3BBeguinot%2C+L%3BVass%2C+W+C%3BWillingham%2C+M+C%3BMerlino%2C+G+T%3BPastan%2C+I%3BLowy%2C+D+R&rft.aulast=Velu&rft.aufirst=T&rft.date=1987-12-04&rft.volume=238&rft.issue=4832&rft.spage=1408&rft.isbn=&rft.btitle=&rft.title=Science+%28New+York%2C+N.Y.%29&rft.issn=00368075&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-06 N1 - Date created - 1988-01-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Alterations in local cerebral glucose utilization induced by phencyclidine. AN - 85231058; pmid-3427457 AB - The effects of phencyclidine (PCP; 0.5, 1,5, 10 mg/kg, i.v.) on local cerebral glucose utilization (LCGU) in the rat were studied with the 2-deoxy-D-[1-14C]glucose method. Significant findings were obtained in 41 of 87 brain regions of PCP-treated rats (25-270% of control). Rates of LCGU increased throughout the limbic system, except the habenula. Although LCGU increased in most sensory structures, it decreased in specific layers of the somatosensory and auditory cortices and the inferior colliculus. Evidence was seen for dissociation between LCGU responses of specific thalamic relay areas and their terminal fields in the cortex. Increases in LCGU occurred throughout the motor system, manifesting a striking pattern of columnar activity in the motor cortex. However, LCGU was reduced in the frontal cortical pole. Elevated LCGU was observed in the pontine nuclei and the nuclei and the nucleus solitarius. Effects of 5 mg/kg PCP diminished with time although 8 regions maintained a metabolic alteration at 180 min. PCP induced several behaviors, including stereotypies, which varied with the dose and time after drug administration. The results demonstrate a PCP-induced activation of various functional circuits in the brain, especially the limbic system, and may provide a physiological basis for PCP's psychotomimetic properties. JF - Brain Research AU - Weissman, A D AU - Dam, M AU - London, E D AD - Addiction Research Center, National Institute on Drug Abuse, Baltimore, MD 21224. PY - 1987 SP - 29 EP - 40 VL - 435 IS - 1-2 SN - 0006-8993, 0006-8993 KW - Carbon Radioisotopes KW - Blood Pressure KW - Animal KW - Brain KW - Organ Specificity KW - Deoxy Sugars KW - Autoradiography KW - Rats KW - Rats, Inbred F344 KW - Phencyclidine KW - Kinetics KW - Deoxyglucose KW - Pulse KW - Male UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85231058?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+Research&rft.atitle=Alterations+in+local+cerebral+glucose+utilization+induced+by+phencyclidine.&rft.au=Weissman%2C+A+D%3BDam%2C+M%3BLondon%2C+E+D&rft.aulast=Weissman&rft.aufirst=A&rft.date=1987-12-01&rft.volume=435&rft.issue=1-2&rft.spage=29&rft.isbn=&rft.btitle=&rft.title=Brain+Research&rft.issn=00068993&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - Mouse keratinocytes derived from initiated skin or papillomas are resistant to DNA strand breakage by benzoyl peroxide: a possible mechanism for tumor promotion mediated by benzoyl peroxide. AN - 81113529; 2445498 AB - Alkaline elution was used to examine DNA single-strand breaks in cultured normal and carcinogen-altered mouse keratinocytes exposed to 12-O-tetradecanoyl phorbol-13-acetate and benzoyl peroxide. Seven cell lines derived from carcinogen-induced mouse skin papillomas and three cell lines derived from N-methyl-N'-nitro-N-nitrosoguanidine-treated non-tumor bearing mouse skin were resistant to phorbol ester-mediated DNA strand breaks after 6 or 24 h. Normal keratinocytes sustained strand breaks after 24 h but not after 6 h. Benzoyl peroxide induced extensive strand breaks in normal keratinocytes at both 6 and 24 h, and this was associated with marked cytotoxicity. In contrast, 9 of 10 cell lines showed complete or partial resistance to strand breaks following benzoyl peroxide exposure. It is proposed that differential resistance to DNA strand breaks and cytotoxicity among normal and carcinogen-altered keratinocytes provides the biological basis for the promoting action of benzoyl peroxide. Furthermore, sublethal DNA damage in preneoplastic or neoplastic keratinocytes may account for the potency of benzoyl peroxide in causing malignant conversion. JF - Carcinogenesis AU - Hartley, J A AU - Gibson, N W AU - Kilkenny, A AU - Yuspa, S H AD - Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1827 EP - 1830 VL - 8 IS - 12 SN - 0143-3334, 0143-3334 KW - Peroxides KW - 0 KW - Methylnitronitrosoguanidine KW - 12H3O2UGSF KW - Keratins KW - 68238-35-7 KW - Benzoyl Peroxide KW - W9WZN9A0GM KW - Index Medicus KW - Animals KW - Mice KW - Mice, Inbred BALB C KW - Skin Neoplasms -- genetics KW - Skin -- drug effects KW - Skin Neoplasms -- chemically induced KW - Skin -- cytology KW - Papilloma -- genetics KW - Papilloma -- chemically induced KW - DNA Damage -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81113529?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Mouse+keratinocytes+derived+from+initiated+skin+or+papillomas+are+resistant+to+DNA+strand+breakage+by+benzoyl+peroxide%3A+a+possible+mechanism+for+tumor+promotion+mediated+by+benzoyl+peroxide.&rft.au=Hartley%2C+J+A%3BGibson%2C+N+W%3BKilkenny%2C+A%3BYuspa%2C+S+H&rft.aulast=Hartley&rft.aufirst=J&rft.date=1987-12-01&rft.volume=8&rft.issue=12&rft.spage=1827&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Increased fragile sites and sister chromatid exchanges in bone marrow and peripheral blood of young cigarette smokers. AN - 81111055; 3677077 AB - Cigarette smoking is considered to be the single most important acquired cause of cancer mortality. Studies of chromosome aberrations, sister chromatid exchanges, and fragile sites in peripheral blood or bone marrow are useful methods to detect the effects of the environmental mutagens or carcinogens found in cigarette smoke. The effects of smoking on the immature cells in the bone marrow have not been studied. Here, we examine the peripheral blood and bone marrow in 18 smokers (15 females and 3 males) with a median age of 25 years (range, 21-40) and an average cigarette use corresponding to 6 pack years. In both bone marrow cells and peripheral blood lymphocytes, we were able to show a significantly increased frequency of sister chromatid exchanges in smokers with a 5 or more cigarette pack year history, but not in those who smoked less than 5 pack years. We also found a higher frequency of sister chromatid exchanges in peripheral blood lymphocytes than in bone marrow cells. In addition, the peripheral lymphocytes of smokers demonstrated (a) a significantly higher frequency of fragile sites, (b) an increased number of metaphases with extensive breakage; and (c) elevated expression of fragile sites at the cancer breakpoints 3p14.2, 11q13.3, 22q12.2, and 11p13-p14.2 and at the oncogene sites bcl 1, erb B, erb A, and sis. Our results suggest that chromosomal DNA of peripheral blood lymphocytes is sensitive to cigarette smoking. Studies of the chromosomal changes in these cells provide an index of the mutagenic damage caused by these exogenous agents in individual patients and the ability of individuals to repair that damage, and might predict susceptibility to malignant events. JF - Cancer research AU - Kao-Shan, C S AU - Fine, R L AU - Whang-Peng, J AU - Lee, E C AU - Chabner, B A AD - Medicine Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12/01/ PY - 1987 DA - 1987 Dec 01 SP - 6278 EP - 6282 VL - 47 IS - 23 SN - 0008-5472, 0008-5472 KW - Index Medicus KW - Age Factors KW - Humans KW - Adult KW - Chromosome Aberrations KW - Male KW - Female KW - Bone Marrow Cells KW - Smoking KW - Sister Chromatid Exchange KW - Blood Cells -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81111055?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Increased+fragile+sites+and+sister+chromatid+exchanges+in+bone+marrow+and+peripheral+blood+of+young+cigarette+smokers.&rft.au=Kao-Shan%2C+C+S%3BFine%2C+R+L%3BWhang-Peng%2C+J%3BLee%2C+E+C%3BChabner%2C+B+A&rft.aulast=Kao-Shan&rft.aufirst=C&rft.date=1987-12-01&rft.volume=47&rft.issue=23&rft.spage=6278&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Differential regulation of c-Ha-ras and c-Ki-ras gene expression in rat mammary gland. AN - 81110424; 2824087 AB - Steady-state levels of c-Has-ras and c-Ki-ras RNA in gestating and lactating rat mammary gland were measured. The c-Ha-ras-specific RNA level increased during gestation and declined with the onset of lactation. On the contrary, the level of c-Ki-ras RNA remained unchanged both during gestation and lactation. There was no change in the level of ras-transcripts in either growing or regressing 7,12-dimethylbenz[a]anthracene-induced mammary tumors. Intravenous infusion of prolactin or implantation of pituitary-derived tumor, which secretes mammotropic hormone, in virgin female rats resulted in decreased levels of c-Ha-ras RNA and no change in c-Ki-ras RNA levels. Our results suggest the transcription of c-Ha-ras and c-Ki-ras genes is differentially regulated. JF - Carcinogenesis AU - Nieto, A AU - Qasba, P K AU - Nakhasi, H L AU - Dhar, R AD - Laboratory of Molecular Virology, NCI. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1955 EP - 1958 VL - 8 IS - 12 SN - 0143-3334, 0143-3334 KW - RNA, Neoplasm KW - 0 KW - 9,10-Dimethyl-1,2-benzanthracene KW - 57-97-6 KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - Deoxyribonuclease HindIII KW - Index Medicus KW - Rats KW - Animals KW - DNA Restriction Enzymes -- metabolism KW - Mammary Neoplasms, Experimental -- genetics KW - RNA, Neoplasm -- analysis KW - Female KW - Pregnancy KW - Lactation KW - Mammary Glands, Animal -- analysis KW - Oncogenes KW - Gene Expression Regulation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81110424?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Differential+regulation+of+c-Ha-ras+and+c-Ki-ras+gene+expression+in+rat+mammary+gland.&rft.au=Nieto%2C+A%3BQasba%2C+P+K%3BNakhasi%2C+H+L%3BDhar%2C+R&rft.aulast=Nieto&rft.aufirst=A&rft.date=1987-12-01&rft.volume=8&rft.issue=12&rft.spage=1955&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Analysis of the rasH oncogene and its p21 product in chemically induced skin tumors and tumor-derived cell lines. AN - 81109734; 2824083 AB - Mouse skin papillomas and squamous cell carcinomas induced by initiation with 7,12-dimethylbenz[a]anthracene and promotion with phorbol esters, such as 12-O-tetradecanoyl phorbol-13-acetate, frequently contain an activated Harvey ras gene. Six murine epidermal cells lines established from pooled skin papillomas previously tested negative in the NIH-3T3 assay, but have an altered differentiation program by a variety of criteria. The Harvey ras gene and its p21 protein product from these cell lines have been analyzed for alterations responsible for their altered growth and differentiation properties that were undetectable by 3T3 transfection assays. In comparison with primary papillomas and carcinomas, shown to have a point mutation in codon 61 of the Harvey ras gene, resulting in a p21 product with the diagnostic alteration in SDS-PAGE, the papilloma cell lines exhibited neither the codon 61 mutation, nor p21 product with altered migration in SDS-PAGE. These findings suggest that these papilloma cell lines contain a genetic lesion(s), other than Harvey ras activation, that may be responsible for their altered epithelial differentiation patterns and thus may serve as a useful model for identifying lesions involved in malignant conversion. JF - Carcinogenesis AU - Harper, J R AU - Reynolds, S H AU - Greenhalgh, D A AU - Strickland, J E AU - Lacal, J C AU - Yuspa, S H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, MD. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1821 EP - 1825 VL - 8 IS - 12 SN - 0143-3334, 0143-3334 KW - DNA, Neoplasm KW - 0 KW - Proto-Oncogene Proteins KW - 9,10-Dimethyl-1,2-benzanthracene KW - 57-97-6 KW - DNA Restriction Enzymes KW - EC 3.1.21.- KW - endodeoxyribonuclease XBAI KW - Deoxyribonucleases, Type II Site-Specific KW - EC 3.1.21.4 KW - Proto-Oncogene Proteins p21(ras) KW - EC 3.6.5.2 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Animals KW - Papilloma -- analysis KW - Polymorphism, Genetic KW - Carcinoma, Squamous Cell -- chemically induced KW - Mice KW - Papilloma -- genetics KW - Carcinoma, Squamous Cell -- analysis KW - DNA Restriction Enzymes -- metabolism KW - Transfection KW - Carcinoma, Squamous Cell -- genetics KW - Papilloma -- chemically induced KW - Cell Line KW - Female KW - Skin Neoplasms -- genetics KW - Oncogenes KW - Proto-Oncogene Proteins -- analysis KW - Skin Neoplasms -- chemically induced KW - Skin Neoplasms -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81109734?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Analysis+of+the+rasH+oncogene+and+its+p21+product+in+chemically+induced+skin+tumors+and+tumor-derived+cell+lines.&rft.au=Harper%2C+J+R%3BReynolds%2C+S+H%3BGreenhalgh%2C+D+A%3BStrickland%2C+J+E%3BLacal%2C+J+C%3BYuspa%2C+S+H&rft.aulast=Harper&rft.aufirst=J&rft.date=1987-12-01&rft.volume=8&rft.issue=12&rft.spage=1821&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Comparison of production of transforming growth factor-beta and platelet-derived growth factor by normal human mesothelial cells and mesothelioma cell lines. AN - 81107670; 3479241 AB - Steady state mRNA levels for transforming growth factor beta, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor beta was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor beta than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A- and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma. JF - Cancer research AU - Gerwin, B I AU - Lechner, J F AU - Reddel, R R AU - Roberts, A B AU - Robbins, K C AU - Gabrielson, E W AU - Harris, C C AD - Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12/01/ PY - 1987 DA - 1987 Dec 01 SP - 6180 EP - 6184 VL - 47 IS - 23 SN - 0008-5472, 0008-5472 KW - Culture Media KW - 0 KW - Peptides KW - Platelet-Derived Growth Factor KW - RNA, Messenger KW - Transforming Growth Factors KW - 76057-06-2 KW - Index Medicus KW - RNA, Messenger -- metabolism KW - Mitosis KW - Humans KW - Gene Expression Regulation KW - Epithelium -- metabolism KW - Peptides -- genetics KW - Cell Line KW - Peptide Biosynthesis KW - Mesothelioma -- metabolism KW - Platelet-Derived Growth Factor -- genetics KW - Pleura -- metabolism KW - Platelet-Derived Growth Factor -- biosynthesis KW - Pleura -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81107670?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Comparison+of+production+of+transforming+growth+factor-beta+and+platelet-derived+growth+factor+by+normal+human+mesothelial+cells+and+mesothelioma+cell+lines.&rft.au=Gerwin%2C+B+I%3BLechner%2C+J+F%3BReddel%2C+R+R%3BRoberts%2C+A+B%3BRobbins%2C+K+C%3BGabrielson%2C+E+W%3BHarris%2C+C+C&rft.aulast=Gerwin&rft.aufirst=B&rft.date=1987-12-01&rft.volume=47&rft.issue=23&rft.spage=6180&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Plasma hyperosmolality increases G protein and 3',5'-cyclic adenosine monophosphate synthesis in the paraventricular and supraoptic nuclei. AN - 78067310; 2856404 AB - Hyperosmotic stimuli produce profound changes in cellular morphology and biosynthetic activities within the hypothalamic paraventricular and supraoptic nuclei (SON) of the rat. The mechanisms by which osmoreceptive signals are transduced within these nuclei are poorly understood. We examined several components of the cAMP-associated second messenger system after giving rats 2% saline to drink for one week, a strong hyperosmotic stimulus. We found that mRNA levels for both the stimulatory and inhibitory guanine-nucleotide binding protein alpha-subunits were increased in the paraventricular nucleus and SON. In the SON, these changes were accompanied by increased basal cAMP levels, cholera toxin-stimulated adenylate cyclase, and Gs alpha. Our results suggest that Gs alpha levels are not saturated with respect to adenylate cyclase coupling and that osmoreception activates the cAMP second messenger system. JF - Molecular endocrinology (Baltimore, Md.) AU - Young, W S AU - Shepard, E A AU - Burch, R M AD - Laboratory of Cell Biology, National Institute of Mental Health Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 884 EP - 888 VL - 1 IS - 12 SN - 0888-8809, 0888-8809 KW - Oligonucleotide Probes KW - 0 KW - RNA, Messenger KW - Adenosine Diphosphate Ribose KW - 20762-30-5 KW - Cholera Toxin KW - 9012-63-9 KW - Cyclic AMP KW - E0399OZS9N KW - GTP-Binding Proteins KW - EC 3.6.1.- KW - Adenylyl Cyclases KW - EC 4.6.1.1 KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Osmolar Concentration KW - Animals KW - Cholera Toxin -- pharmacology KW - Adenylyl Cyclases -- metabolism KW - Nucleic Acid Hybridization KW - RNA, Messenger -- genetics KW - Adenosine Diphosphate Ribose -- metabolism KW - Male KW - Catalysis KW - Plasma -- physiology KW - Paraventricular Hypothalamic Nucleus -- metabolism KW - Cyclic AMP -- biosynthesis KW - GTP-Binding Proteins -- biosynthesis KW - Supraoptic Nucleus -- metabolism KW - Cyclic AMP -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78067310?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+endocrinology+%28Baltimore%2C+Md.%29&rft.atitle=Plasma+hyperosmolality+increases+G+protein+and+3%27%2C5%27-cyclic+adenosine+monophosphate+synthesis+in+the+paraventricular+and+supraoptic+nuclei.&rft.au=Young%2C+W+S%3BShepard%2C+E+A%3BBurch%2C+R+M&rft.aulast=Young&rft.aufirst=W&rft.date=1987-12-01&rft.volume=1&rft.issue=12&rft.spage=884&rft.isbn=&rft.btitle=&rft.title=Molecular+endocrinology+%28Baltimore%2C+Md.%29&rft.issn=08888809&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1990-12-05 N1 - Date created - 1990-12-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Testicular cancer in young men: the search for causes of the epidemic increase in the United States. AN - 78045176; 2901454 AB - A case-control study of 271 men with testicular cancer and 259 controls was conducted in the Washington, DC area to evaluate whether suggested risk factors could be responsible for the epidemic increases in testicular cancer in young men. No substantial risks were associated with a history of groin hernia operation, the common childhood diseases, allergies, x rays below the waist, venereal disease, vasectomy, or external means of elevating the temperature of the testis. Excess risks were associated with a history of undescended testis (RR = 3.7, CI = 1.5-9.5), testicular trauma (RR = 2.6, CI = 1.6-4.2), and mumps orchitis (RR = 5.8, CI = 0.7-129.7). It is unlikely, however, that any of these conditions has increased sufficiently over time to markedly affect the testicular cancer incidence patterns. Therefore, while the risk factors identified in this paper are of epidemiological interest, they do not account for the increase in testicular cancer in young men. JF - Journal of epidemiology and community health AU - Brown, L M AU - Pottern, L M AU - Hoover, R N AD - Epidemiology and Biostatics Program, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 349 EP - 354 VL - 41 IS - 4 SN - 0143-005X, 0143-005X KW - Index Medicus KW - District of Columbia KW - Cryptorchidism -- complications KW - Testis -- injuries KW - Risk Factors KW - Humans KW - Testis -- pathology KW - Adult KW - Smoking -- adverse effects KW - Clothing -- adverse effects KW - Maryland KW - Adolescent KW - Male KW - Testicular Neoplasms -- pathology KW - Testicular Neoplasms -- etiology KW - Testicular Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78045176?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+epidemiology+and+community+health&rft.atitle=Testicular+cancer+in+young+men%3A+the+search+for+causes+of+the+epidemic+increase+in+the+United+States.&rft.au=Brown%2C+L+M%3BPottern%2C+L+M%3BHoover%2C+R+N&rft.aulast=Brown&rft.aufirst=L&rft.date=1987-12-01&rft.volume=41&rft.issue=4&rft.spage=349&rft.isbn=&rft.btitle=&rft.title=Journal+of+epidemiology+and+community+health&rft.issn=0143005X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-10-27 N1 - Date created - 1988-10-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Epidemiol. 1986 Jun;15(2):164-70 [3721677] J Natl Cancer Inst. 1985 Feb;74(2):377-81 [2857796] Int J Epidemiol. 1984 Sep;13(3):311-8 [6149198] J Natl Cancer Inst. 1983 Dec;71(6):1151-5 [6140323] Br J Cancer. 1982 Nov;46(5):749-56 [6128995] J Natl Cancer Inst. 1980 Nov;65(5):1055-61 [6933238] Cancer Res. 1986 Sep;46(9):4812-6 [3731127] Br J Urol. 1963 Mar;35:67-9 [14031378] N Engl J Med. 1980 Jul 10;303(2):112-3 [6104291] Int J Cancer. 1979 May 15;23(5):598-602 [37169] Am J Epidemiol. 1978 Jul;108(1):78-9 [685980] Mayo Clin Proc. 1977 Jan;52(1):3-7 [609284] Eur J Pediatr. 1977 Aug 23;126(1-2):77-84 [20308] Br Med J. 1977 Jul 16;2(6080):191 [871846] J Natl Cancer Inst. 1977 May;58(5):1255-61 [192897] Am J Epidemiol. 1980 Aug;112(2):232-46 [6106385] J Natl Cancer Inst. 1976 Apr;56(4):731-3 [3662] Br J Cancer. 1974 Feb;29(2):176-8 [4830140] Cancer. 1969 May;23(5):1119-21 [5781507] Am J Epidemiol. 1976 Nov;104(5):511-6 [988743] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Issues in biochemical applications to risk assessment: when can lymphocytes be used as surrogate markers? AN - 78020471; 2834193 JF - Environmental health perspectives AU - Lucier, G W AU - Thompson, C L AD - Laboratory of Biochemical Risk Analysis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 187 EP - 191 VL - 76 SN - 0091-6765, 0091-6765 KW - Biomarkers, Tumor KW - 0 KW - Carcinogens KW - Mutagens KW - Proteins KW - Receptors, Cell Surface KW - Index Medicus KW - Risk KW - Mutagens -- analysis KW - DNA Damage KW - Humans KW - Proteins -- metabolism KW - Mutation KW - DNA Repair -- drug effects KW - Receptors, Cell Surface -- drug effects KW - Cytogenetics KW - Carcinogens -- toxicity KW - Biomarkers, Tumor -- analysis KW - Lymphocytes -- metabolism KW - Lymphocytes -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78020471?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Issues+in+biochemical+applications+to+risk+assessment%3A+when+can+lymphocytes+be+used+as+surrogate+markers%3F&rft.au=Lucier%2C+G+W%3BThompson%2C+C+L&rft.aulast=Lucier&rft.aufirst=G&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Carcinog Compr Surv. 1985;10:511-28 [4064016] J Natl Cancer Inst. 1987 May;78(5):887-98 [3471998] Environ Health Perspect. 1987 Dec;76:141-5 [3447891] Environ Health Perspect. 1987 Dec;76:79-87 [2834196] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Issues in biochemical applications to risk assessment: how do we evaluate individual components of multistage models? AN - 78011543; 3447895 JF - Environmental health perspectives AU - Anderson, M W AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 175 EP - 179 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Index Medicus KW - Risk KW - Animals KW - Mutagenicity Tests KW - DNA Damage KW - Mutation KW - Cell Transformation, Neoplastic KW - Carcinogens -- administration & dosage KW - Neoplasms, Experimental -- chemically induced KW - Carcinogens -- toxicity KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78011543?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Issues+in+biochemical+applications+to+risk+assessment%3A+how+do+we+evaluate+individual+components+of+multistage+models%3F&rft.au=Anderson%2C+M+W&rft.aulast=Anderson&rft.aufirst=M&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=175&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 1987 Dec;76:57-63 [3447904] Environ Health Perspect. 1987 Dec;76:163-7 [3447893] Nature. 1983 Jul 7-13;304(5921):67-9 [6866091] Environ Health Perspect. 1987 Dec;76:49-56 [2896122] Science. 1987 Sep 11;237(4820):1309-16 [3629242] Environ Health Perspect. 1987 Dec;76:9-14 [3447906] Environ Health Perspect. 1985 Oct;62:89-94 [4085451] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Implications of pharmacokinetic modeling in risk assessment analysis. AN - 78003716; 3447907 AB - Physiologic pharmacokinetic models are a useful interface between exposure models and risk assessment models by providing a means to estimate tissue concentrations of reactive chemical species at the site of action. The models utilize numerous parameters that can be characterized as anatomical, such as body size or tissue volume; physiological, such as tissue blood perfusion rates, clearances, and metabolism; thermodynamic, such as partition coefficients; and transport, such as membrane permeabilities. The models provide a format to investigate how these parameters can influence the disposition of chemicals throughout the body, which is an important consideration in interpreting toxicity studies. Physiologic models can take into account nonlinear effects related to clearance, metabolism, or transport. They allow for extrapolation of tissue concentration from high dose to low dose experiments and from species to species and can account for temporal variations in dose. JF - Environmental health perspectives AU - Lutz, R J AU - Dedrick, R L AD - Chemical Engineering Section, National Institutes of Health, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 97 EP - 106 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Index Medicus KW - Risk KW - Animals KW - Thermodynamics KW - Dose-Response Relationship, Drug KW - Humans KW - Metabolic Clearance Rate KW - Body Composition KW - Biological Transport, Active KW - Carcinogens -- administration & dosage KW - Carcinogens -- pharmacokinetics KW - Carcinogens -- toxicity KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78003716?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Implications+of+pharmacokinetic+modeling+in+risk+assessment+analysis.&rft.au=Lutz%2C+R+J%3BDedrick%2C+R+L&rft.aulast=Lutz&rft.aufirst=R&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=97&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Drug Metab Dispos. 1977 Jul-Aug;5(4):386-96 [19218] Cancer Chemother Rep. 1975 Jul-Aug;59(4):795-804 [1236771] Toxicol Appl Pharmacol. 1978 Jun;44(3):581-91 [567390] J Environ Pathol Toxicol. 1978 Sep-Oct;1(1):163-79 [722184] Fed Proc. 1980 Jan;39(1):54-9 [6766113] Cancer Treat Rep. 1979 Nov-Dec;63(11-12):1939-47 [118803] Toxicol Appl Pharmacol. 1980 Aug;55(1):154-61 [7423500] J Pharmacol Exp Ther. 1981 Dec;219(3):669-74 [7299690] J Pharmacokinet Biopharm. 1982 Feb;10(1):53-75 [7069578] J Pharm Pharmacol. 1982 Sep;34(9):597-600 [6127390] Science. 1983 Mar 4;219(4588):1032-7 [6823565] J Pharmacokinet Biopharm. 1982 Dec;10(6):637-47 [7182459] Drug Metab Dispos. 1984 Sep-Oct;12(5):527-35 [6149901] Biochem Pharmacol. 1973 Oct 1;22(19):2405-17 [4200888] Biochem Pharmacol. 1972 Jan;21(1):1-16 [4500983] Science. 1949 Jun 10;109(2841):579-85 [17835379] J Pharmacokinet Biopharm. 1973 Oct;1(5):435-61 [4787619] Arch Environ Contam Toxicol. 1974 Mar;2(1):9-42 [4828556] J Pharmacol Exp Ther. 1974 Jun;189(3):585-92 [4843162] Cancer Res. 1977 Oct;37(10):3475-83 [908002] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Statistical properties of a two-stage model of carcinogenesis. AN - 78003566; 3447890 AB - Some of the statistical properties of a simple two-stage model of carcinogenesis are explored. The implications of additive treatment effects versus independent treatment effects on the shape of the dose-response curve are considered. Response that is low-dose linear results in the cases where the mutation rates are affected by dose or in the cases where treatment changes the birth rate/death rate of initiated cells in an additive fashion. Independent treatment effects lead to non-low-dose linear response when the survival of initiated cells is affected by treatment. A computer simulation experiment was performed that examined the ability of animal carcinogenesis data to differentiate between various forms of this simple two-stage model. It is shown that animal carcinogenicity experiments do not contain enough data to adequately describe the difference between these two types of effects. JF - Environmental health perspectives AU - Portier, C J AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 125 EP - 131 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Index Medicus KW - Risk KW - Animals KW - Carcinogens -- administration & dosage KW - Dose-Response Relationship, Drug KW - Carcinogens -- toxicity KW - Clone Cells -- drug effects KW - Cocarcinogenesis KW - Neoplasms, Experimental -- chemically induced KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78003566?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Statistical+properties+of+a+two-stage+model+of+carcinogenesis.&rft.au=Portier%2C+C+J&rft.aulast=Portier&rft.aufirst=C&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=125&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Natl Cancer Inst. 1980 Sep;65(3):559-69 [6931935] Yale J Biol Med. 1980 Sep-Oct;53(5):367-84 [7013284] J Natl Cancer Inst. 1981 Jun;66(6):1037-52 [6941039] Br J Cancer. 1981 Jul;44(1):1-14 [6789853] Cancer Res. 1978 Mar;38(3):865-6 [626986] J Natl Cancer Inst. 1979 Mar;62(3):493-501 [283278] Am J Hum Genet. 1979 Nov;31(6):704-10 [517520] Biometrics. 1981 Jun;37(2):353-66 [7023560] Environ Health Perspect. 1983 Apr;50:285-91 [6873019] Cancer Res. 1985 Apr;45(4):1437-43 [2983882] Cancer Res. 1986 Sep;46(9):4372-8 [3731095] Risk Anal. 1987 Mar;7(1):109-19 [3615992] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of time-variant exposure on toxic substance response. AN - 78000555; 3329094 AB - Sources of time-variant exposure to toxic substances are identified and examined for their effects on the estimation of response. It is shown that only time-averaged target tissue concentrations are required to obtain rigorous risk estimates from the one-hit and multihit models. In contrast, detailed concentration histories need to be retained throughout analyses involving two-event models with intermediate-stage clonal growth advantage (clonal two-stage) and multistage models. Cumulative incidence ratios, based on the exact to time-averaged treatment of concentration time dependencies, are evaluated for substances whose toxic responses exhibit moderate (arsenic) and strong (ethylene dibromide) dependence on time of actual exposure. These ratios reveal that time-averaged dose approximations may lead to several orders of magnitude error in both the multistage and clonal two-stage models if exposure periods are short, and that 3.4-fold (arsenic) and 8-fold (ethylene dibromide) errors still exist even when an actual two-thirds lifetime exposure is averaged over a full lifetime. Finally, the effects of time-variant exposure on risk estimation due to migration and birth-death in an epidemiological setting are examined. A residence time distribution calculation shows that, if these effects are ignored for a population orally exposed to arsenic and characterized by an out-migration rate in excess of 5%/yr, response errors will exceed an order of magnitude. JF - Environmental health perspectives AU - Morrison, P F AD - Biomedical Engineering and Instrumentation Branch, National Institutes of Health, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 133 EP - 139 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Ethylene Dibromide KW - 1N41638RNO KW - Arsenic KW - N712M78A8G KW - Index Medicus KW - Animals KW - Arsenic -- toxicity KW - Ethylene Dibromide -- administration & dosage KW - Epidemiologic Methods KW - Humans KW - Ethylene Dibromide -- toxicity KW - Arsenic -- administration & dosage KW - Time Factors KW - Models, Biological KW - Carcinogens -- administration & dosage KW - Carcinogens -- pharmacokinetics KW - Neoplasms -- chemically induced KW - Neoplasms -- epidemiology KW - Carcinogens -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/78000555?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Effects+of+time-variant+exposure+on+toxic+substance+response.&rft.au=Morrison%2C+P+F&rft.aulast=Morrison&rft.aufirst=P&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=133&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Natl Cancer Inst. 1980 Apr;64(4):977-89 [6929006] Biometrics. 1977 Mar;33(1):1-30 [843567] J Natl Cancer Inst. 1980 Sep;65(3):559-69 [6931935] Drug Metab Dispos. 1984 Sep-Oct;12(5):527-35 [6149901] Environ Health Perspect. 1977 Aug;19:109-19 [908285] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Placental markers of human exposure to polychlorinated biphenyls and polychlorinated dibenzofurans. AN - 77997106; 2834196 AB - Our studies have evaluated biochemical changes in placentae from humans exposed to rice oil contaminated with polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) in Taiwan. Placentae were obtained from nonsmoking women 4 to 5 years after the exposure had occurred. The exposed individuals ingested approximately 1 to 3 g PCBs and 5 mg PCDFs, and many exhibited symptoms characteristic of PCB poisoning. This disease was termed "Yu-Cheng" in Chinese. Based on data from experimental animal models, we examined a number of parameters in placentae from control and exposed women, including arylhydrocarbon hydroxylase (AHH) activity, cytochrome P-450 isozymes, epidermal growth factor (EGF) receptor binding properties and actions, and Ah receptor. We also quantified concentrations of various PCB and PCDF congeners known to be present in the contaminated rice oil. Our results revealed a dramatic elevation in placental AHH activity in samples from PCB/PCDF-exposed women. This increase in enzyme activity was associated with a parallel increase in placental microsomal protein immunochemically related to cytochrome P-450 form 6 [derived from 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced rabbit lung]. No other cytochrome P-450 isozyme was detected in placental preparations, and the form 6 homolog was found only in placentae from exposed women. EGF receptor-mediated autophosphorylation capacity was significantly diminished in PCB/PCDF placentae, but this effect was not associated with changes in plasma membrane EGF receptor binding properties (Kd and Bmax). The EGF receptor autophosphorylation effect correlated well with the decrease in birthweight observed in offspring of exposed women, suggesting that this biochemical event might provide a good marker of effect for the toxic halogenated aromatics. JF - Environmental health perspectives AU - Lucier, G W AU - Nelson, K G AU - Everson, R B AU - Wong, T K AU - Philpot, R M AU - Tiernan, T AU - Taylor, M AU - Sunahara, G I AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 79 EP - 87 VL - 76 SN - 0091-6765, 0091-6765 KW - Benzofurans KW - 0 KW - Dibenzofurans, Polychlorinated KW - Isoenzymes KW - Plant Oils KW - Receptors, Aryl Hydrocarbon KW - Receptors, Drug KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Receptor, Epidermal Growth Factor KW - EC 2.7.10.1 KW - Index Medicus KW - Receptor, Epidermal Growth Factor -- drug effects KW - Receptors, Drug -- metabolism KW - Receptor, Epidermal Growth Factor -- metabolism KW - Receptors, Drug -- drug effects KW - Humans KW - Adult KW - Cytochrome P-450 Enzyme System -- metabolism KW - Adolescent KW - Isoenzymes -- metabolism KW - Female KW - Pregnancy KW - Food Contamination KW - Polychlorinated Biphenyls -- poisoning KW - Placenta -- drug effects KW - Oryza -- poisoning KW - Benzofurans -- poisoning KW - Placenta -- metabolism KW - Plant Oils -- poisoning UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77997106?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Placental+markers+of+human+exposure+to+polychlorinated+biphenyls+and+polychlorinated+dibenzofurans.&rft.au=Lucier%2C+G+W%3BNelson%2C+K+G%3BEverson%2C+R+B%3BWong%2C+T+K%3BPhilpot%2C+R+M%3BTiernan%2C+T%3BTaylor%2C+M%3BSunahara%2C+G+I&rft.aulast=Lucier&rft.aufirst=G&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=79&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Cell Endocrinol. 1983 Jul;31(1):1-19 [6309581] J Biol Chem. 1978 Dec 10;253(23):8640-7 [101550] Am J Ind Med. 1984;5(1-2):71-9 [6230931] Cancer Res. 1984 Sep;44(9):3916-23 [6611202] Mol Pharmacol. 1984 Jul;26(1):90-8 [6087120] Toxicology. 1984 Aug;32(2):131-44 [6464025] Proc Natl Acad Sci U S A. 1984 Dec;81(23):7407-11 [6095293] Lancet. 1985 Mar 30;1(8431):721-4 [2857997] Biochem Biophys Res Commun. 1977 Jun 6;76(3):644-51 [409392] Mol Pharmacol. 1987 Nov;32(5):572-8 [3119985] Drug Metab Dispos. 1975 Sep-Oct;3(5):371-80 [241618] J Biol Chem. 1976 Aug 25;251(16):4936-46 [956169] Science. 1968 May 3;160(3827):541-2 [5644060] CRC Crit Rev Biochem. 1979;6(4):401-37 [378536] Proc Natl Acad Sci U S A. 1979 Oct;76(10):5168-72 [315558] Med Biol. 1979 Oct;57(5):306-12 [522518] Arch Biochem Biophys. 1980 Apr 1;200(2):513-23 [7436419] Bull Environ Contam Toxicol. 1981 Apr;26(4):489-95 [6786399] Arch Environ Health. 1981 Nov-Dec;36(6):321-6 [6797353] Biochem Biophys Res Commun. 1981 Dec 31;103(4):1310-7 [7332594] Dev Pharmacol Ther. 1982;5(3-4):162-72 [7151648] Teratology. 1982 Dec;26(3):259-61 [6819644] Science. 1985 Mar 22;227(4693):1499-502 [3856321] Toxicol Appl Pharmacol. 1985 Feb;77(2):251-9 [2579474] Environ Health Perspect. 1985 Feb;59:121-8 [2985378] Environ Health Perspect. 1985 Feb;59:17-29 [3921359] Environ Health Perspect. 1985 Feb;59:59-65 [3921366] Cancer Res. 1986 Feb;46(2):999-1004 [3079671] J Biol Chem. 1986 Jun 25;261(18):8295-7 [3013845] Toxicol Appl Pharmacol. 1986 Aug;85(1):60-8 [3088776] J Biol Chem. 1984 Jan 25;259(2):980-5 [6319394] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Issues in biochemical applications to risk assessment: are short-term tests predictive of in vivo tumorigenicity? AN - 77994277; 3447893 JF - Environmental health perspectives AU - Tennant, R W AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 163 EP - 167 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Mutagens KW - Index Medicus KW - Salmonella -- drug effects KW - Risk KW - Animals KW - Mutagenicity Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77994277?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Issues+in+biochemical+applications+to+risk+assessment%3A+are+short-term+tests+predictive+of+in+vivo+tumorigenicity%3F&rft.au=Tennant%2C+R+W&rft.aulast=Tennant&rft.aufirst=R&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=163&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Science. 1987 May 22;236(4804):933-41 [3554512] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Issues concerning the estimation of the TD50. AN - 77993555; 3444931 AB - The TD50 (or tumorigenic dose rate 50) is a generally accepted measure of the carcinogenic potency of a chemical in a particular strain of animal. This paper discusses error in the estimation of the TD50 caused by intercurrent mortality and error resulting from consideration of only significant TD50's. Using computer simulations, we found that treatment-related toxicity had only a small effect on estimating the TD50, with errors seldom exceeding 5%. The TD50 is sensitive to changes in tumor lethality with errors ranging to as high as 50%. Many of these errors were significantly different from zero and the results suggest that potency estimation could be improved by basing the estimates upon the tumor incidence rate rather than upon the tumor death rate when an estimate of tumor lethality is obtainable. JF - Risk analysis : an official publication of the Society for Risk Analysis AU - Portier, C J AU - Hoel, D G AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 437 EP - 447 VL - 7 IS - 4 SN - 0272-4332, 0272-4332 KW - Carcinogens KW - 0 KW - Index Medicus KW - Rats KW - Animals KW - Dose-Response Relationship, Drug KW - Mice KW - Computer Simulation KW - Neoplasms, Experimental -- chemically induced KW - Carcinogens -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77993555?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Risk+analysis+%3A+an+official+publication+of+the+Society+for+Risk+Analysis&rft.atitle=Issues+concerning+the+estimation+of+the+TD50.&rft.au=Portier%2C+C+J%3BHoel%2C+D+G&rft.aulast=Portier&rft.aufirst=C&rft.date=1987-12-01&rft.volume=7&rft.issue=4&rft.spage=437&rft.isbn=&rft.btitle=&rft.title=Risk+analysis+%3A+an+official+publication+of+the+Society+for+Risk+Analysis&rft.issn=02724332&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-27 N1 - Date created - 1988-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - DNA adducts as a dosimeter for risk estimation. AN - 77992805; 3447903 AB - The dose response for O6-methylguanine (O6MG) formation and cytotoxicity was determined in lung and nasal mucosa from Fischer 344 rats during multiple dose administration of the tobacco-specific nitrosamine-4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK). O6MG accumulated in the lung following treatment for 12 days with doses of NNK from 0.3 to 100 mg/kg/day. The dose response for NNK was nonlinear; the O6MG-to-dose ratio, an index of alkylation efficiency, increased dramatically as the dose of carcinogen decreased. These data suggest that low- and high-Km pathways may exist for activation of NNK to a methylating agent. Marked differences in O6MG concentration were observed in specific lung cell populations. The Clara cell, one of the suggested progenitor cells for nitrosamine-induced neoplasia, was found to possess the greatest concentration of O6MG. Moreover, as the dose of NNK was decreased from 100 to 0.3 mg/kg, the alkylation efficiency in this cell population increased 38-fold. The presence of a high-affinity pathway in the Clara cell for activation of NNK may contribute to the potent carcinogenicity observed following low-dose exposure to this tobacco-specific carcinogen. The dose response for O6MG formation differed considerably between the respiratory and olfactory mucosa from the nasal passages of the rat. The dose response was nonlinear in respiratory mucosa but linear in olfactory mucosa. The alkylation efficiency increased dramatically only in the respiratory mucosa as the dose of NNK was decreased. These studies suggest that a low Km pathway for NNK activation is also present in the nose and that this pathway is localized predominantly in the respiratory region.(ABSTRACT TRUNCATED AT 250 WORDS) JF - Environmental health perspectives AU - Belinsky, S A AU - White, C M AU - Devereux, T R AU - Anderson, M W AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 3 EP - 8 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Nitrosamines KW - Guanine KW - 5Z93L87A1R KW - 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone KW - 7S395EDO61 KW - DNA KW - 9007-49-2 KW - O-(6)-methylguanine KW - 9B710FV2AE KW - Index Medicus KW - Animals KW - Nitrosamines -- toxicity KW - Dose-Response Relationship, Drug KW - Lung -- metabolism KW - Nasal Mucosa -- drug effects KW - Nasal Mucosa -- metabolism KW - Guanine -- metabolism KW - Alkylation KW - Nitrosamines -- administration & dosage KW - Rats KW - Risk KW - Respiratory Tract Neoplasms -- chemically induced KW - Rats, Inbred F344 KW - Lung -- drug effects KW - Guanine -- analogs & derivatives KW - Carcinogens -- administration & dosage KW - DNA Damage KW - DNA -- metabolism KW - DNA -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77992805?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=DNA+adducts+as+a+dosimeter+for+risk+estimation.&rft.au=Belinsky%2C+S+A%3BWhite%2C+C+M%3BDevereux%2C+T+R%3BAnderson%2C+M+W&rft.aulast=Belinsky&rft.aufirst=S&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Cancer Res Clin Oncol. 1984;108(1):81-6 [6746721] Cancer Res. 1986 Mar;46(3):1280-4 [3943097] Cancer Res. 1987 Feb 15;47(4):1143-8 [3802095] Cancer Res. 1987 Mar 15;47(6):1577-81 [3815358] Cancer Res. 1980 Nov;40(11):4144-50 [7471055] Science. 1983 Mar 4;219(4588):1032-7 [6823565] Proc Natl Acad Sci U S A. 1984 Mar;81(6):1692-5 [6584902] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. AN - 77992790; 2452255 AB - The antigenic properties of the highly purified US reference standard endotoxin (RSE) exposed to varying doses of ionizing radiation were studied with double immunodiffusion, immunoelectrophoresis and immunoblotting. Rabbit RSE antisera identified 2 distinct major antigenic components for untreated RSE: one related to the O-polysaccharide side chain ("O-antigenic specificity"), the other to the R-core. Based on a serologic cross-reactivity of R-core of RSE (Escherichia coli 0113) with the R-core of the lipopolysaccharide from E. coli 0111, the core type of E. coli 0113 was identified as coli R3. Increasing exposure of RSE to ionizing radiation progressively destroyed all antigenic reactivities: at lower doses of radiation the rate of elimination differed for the 2 antigen classes. The O-polysaccharide was more sensitive to gamma-radiation than the R-core and the O-antigenicity was lost before that of the R-core. Endotoxin molecules containing incomplete R-core (radiation-induced or mutant) did not react with the RSE antiserum. JF - Journal of clinical & laboratory immunology AU - Csako, G AU - Suba, E A AU - Tsai, C M AU - Mocca, L F AU - Elin, R J AD - Clinical Pathology Department, National Institutes of Health, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 193 EP - 198 VL - 24 IS - 4 SN - 0141-2760, 0141-2760 KW - Antigens, Bacterial KW - 0 KW - Endotoxins KW - Epitopes KW - Lipopolysaccharides KW - Index Medicus KW - Animals KW - Lipopolysaccharides -- radiation effects KW - Lipopolysaccharides -- immunology KW - Immunoelectrophoresis KW - Gamma Rays KW - Immunodiffusion KW - Epitopes -- radiation effects KW - Electrophoresis, Polyacrylamide Gel KW - Rabbits KW - Dose-Response Relationship, Radiation KW - Epitopes -- immunology KW - Antigens, Bacterial -- radiation effects KW - Endotoxins -- radiation effects KW - Endotoxins -- standards KW - Antigens, Bacterial -- immunology KW - Endotoxins -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77992790?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+%26+laboratory+immunology&rft.atitle=Dose-dependent+changes+in+the+antigenicity+of+bacterial+endotoxin+exposed+to+ionizing+radiation.&rft.au=Csako%2C+G%3BSuba%2C+E+A%3BTsai%2C+C+M%3BMocca%2C+L+F%3BElin%2C+R+J&rft.aulast=Csako&rft.aufirst=G&rft.date=1987-12-01&rft.volume=24&rft.issue=4&rft.spage=193&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+%26+laboratory+immunology&rft.issn=01412760&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-02 N1 - Date created - 1988-06-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cellular and molecular mechanisms of multistep carcinogenesis: relevance to carcinogen risk assessment. AN - 77992599; 3447905 AB - Carcinogenesis is a multistep process involving alterations in at least two distinct classes of genes. Protooncogenes are activated qualitatively or quantitatively in certain tumors, and they appear to act as positive proliferative signals for neoplastic growth. In contrast, tumor suppressor genes are normal genes that must be inactivated or lost for tumor development. When active, tumor suppressor genes control neoplastic growth in a negative manner. Chemicals may influence the carcinogenic process by mutational activation of protooncogenes and/or inactivation of tumor suppressor genes. The types of genetic alterations involved in these mutational events are diverse, and their dose-response curves may be varied. In addition, chemical carcinogens may act on nonmutational processes such as the clonal expansion of premalignant cells. The carcinogenic risk of a specific chemical is a composite of its effects on multiple genetic and epigenetic processes. JF - Environmental health perspectives AU - Barrett, J C AU - Wiseman, R W AD - National Institute of Environmental Health Sciences, Research Traingle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 65 EP - 70 VL - 76 SN - 0091-6765, 0091-6765 KW - Carcinogens KW - 0 KW - Index Medicus KW - Risk KW - Animals KW - Dose-Response Relationship, Drug KW - Humans KW - Suppression, Genetic -- drug effects KW - Models, Biological KW - Proto-Oncogenes -- drug effects KW - Cocarcinogenesis KW - Carcinogens -- administration & dosage KW - Neoplasms -- chemically induced KW - Carcinogens -- toxicity KW - Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77992599?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Cellular+and+molecular+mechanisms+of+multistep+carcinogenesis%3A+relevance+to+carcinogen+risk+assessment.&rft.au=Barrett%2C+J+C%3BWiseman%2C+R+W&rft.aulast=Barrett&rft.aufirst=J&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=65&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer Res. 1985 Apr;45(4):1437-43 [2983882] J Supramol Struct Cell Biochem. 1981;17(2):133-46 [7033553] Nature. 1985 May 30-Jun 5;315(6018):382-5 [3923365] Nature. 1985 Aug 15-21;316(6029):636-9 [2993900] Carcinogenesis. 1985 Nov;6(11):1607-10 [2414025] Science. 1985 Nov 15;230(4727):770-6 [2997917] Cancer Res. 1986 Apr;46(4 Pt 1):1573-80 [2418950] Cancer Res. 1986 Jun;46(6):2863-5 [3084079] Virchows Arch B Cell Pathol Incl Mol Pathol. 1980;33(3):303-4 [6110278] CRC Crit Rev Toxicol. 1974 Jan;2(4):419-43 [4822436] Carcinogenesis. 1981;2(12):1375-9 [7326837] Carcinogenesis. 1982;3(8):895-8 [6812976] Nature. 1976 Mar 4;260(5546):17-20 [1264187] Science. 1978 Jun 30;200(4349):1448-59 [96525] Cancer Res. 1980 Apr;40(4):1194-203 [7357549] Cancer Res. 1983 May;43(5):2034-41 [6403231] J Natl Cancer Inst. 1983 Mar;70(3):455-63 [6572736] Nature. 1983 Jul 7-13;304(5921):67-9 [6866091] Environ Health Perspect. 1983 Apr;50:309-20 [6873021] Nature. 1983 Oct 27-Nov 2;305(5937):779-84 [6633649] Cancer Lett. 1983 Dec;21(2):141-7 [6652618] Science. 1984 Mar 9;223(4640):1028-33 [6320372] Science. 1984 Jun 8;224(4653):1121-4 [6719137] Environ Res. 1984 Aug;34(2):227-41 [6086305] Proc Natl Acad Sci U S A. 1984 Aug;81(15):4940-4 [6589638] Nature. 1986 Jul 3-9;322(6074):78-80 [3014349] Proc Natl Acad Sci U S A. 1986 Aug;83(16):5825-9 [3016723] Proc Natl Acad Sci U S A. 1986 Aug;83(16):5992-6 [3461473] Carcinogenesis. 1986 Nov;7(11):1845-8 [3769132] Science. 1987 Jan 9;235(4785):177-82 [3798106] Science. 1987 Jan 16;235(4786):305-11 [3541204] Proc Natl Acad Sci U S A. 1987 Apr;84(7):2029-32 [3104907] Science. 1987 Sep 11;237(4820):1309-16 [3629242] Cancer Res. 1950 Nov;10(11):713-7 [14783769] Arch Environ Health. 1963 Dec;7:668-74 [14077213] J Natl Cancer Inst. 1985 Apr;74(4):735-40 [3921746] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cancer risk models for ionizing radiation. AN - 77987210; 3447889 AB - Risk estimation in radiation carcinogenesis depends primarily on epidemiological data and hazard rate models. The A-bomb survivors follow-up provides information on the complexity of this process. Several hazard rate models are briefly discussed and illustrated using the A-bomb experience. JF - Environmental health perspectives AU - Hoel, D G AD - National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 121 EP - 124 VL - 76 SN - 0091-6765, 0091-6765 KW - Index Medicus KW - Risk KW - Epidemiologic Methods KW - Nuclear Warfare KW - Humans KW - Leukemia, Radiation-Induced -- etiology KW - Leukemia, Radiation-Induced -- mortality KW - Dose-Response Relationship, Radiation KW - Male KW - Japan KW - Female KW - Neoplasms, Radiation-Induced -- etiology KW - Neoplasms, Radiation-Induced -- mortality KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77987210?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Cancer+risk+models+for+ionizing+radiation.&rft.au=Hoel%2C+D+G&rft.aulast=Hoel&rft.aufirst=D&rft.date=1987-12-01&rft.volume=76&rft.issue=&rft.spage=121&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-06-07 N1 - Date created - 1988-06-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Am J Epidemiol. 1977 Nov;106(5):418-32 [920729] J Natl Cancer Inst. 1980 Aug;65(2):353-76 [6931253] J Epidemiol Community Health. 1984 Jun;38(2):108-12 [6747507] Health Phys. 1987 Jan;52(1):55-63 [3804743] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Failure of diterpenes from Jatropha curcas to induce mutation in Salmonella typhimurium TA98 and TA100. AN - 77959741; 3326080 AB - No mutagenicity of five diterpenes isolated from the roots of Jatropha curcas was detected by Ame's test using both TA98 and TA100 tester strains with or without S-9 fraction from livers of rats treated with polychlorinated biphenyl. JF - Research communications in chemical pathology and pharmacology AU - Rojanapo, W AU - Pimbua, J AU - Glinsukon, T AU - Naengchomnong, W AU - Thebtaranonth, Y AD - Research Division, National Cancer Institute, Bangkok, Thailand. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 397 EP - 400 VL - 58 IS - 3 SN - 0034-5164, 0034-5164 KW - Diterpenes KW - 0 KW - Mutagens KW - Index Medicus KW - Rats KW - Animals KW - Mutagenicity Tests KW - Biotransformation KW - Microsomes, Liver -- metabolism KW - In Vitro Techniques KW - Salmonella typhimurium -- genetics KW - Diterpenes -- pharmacokinetics KW - Plants, Medicinal -- analysis KW - Diterpenes -- toxicity KW - Mutagens -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77959741?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Research+communications+in+chemical+pathology+and+pharmacology&rft.atitle=Failure+of+diterpenes+from+Jatropha+curcas+to+induce+mutation+in+Salmonella+typhimurium+TA98+and+TA100.&rft.au=Rojanapo%2C+W%3BPimbua%2C+J%3BGlinsukon%2C+T%3BNaengchomnong%2C+W%3BThebtaranonth%2C+Y&rft.aulast=Rojanapo&rft.aufirst=W&rft.date=1987-12-01&rft.volume=58&rft.issue=3&rft.spage=397&rft.isbn=&rft.btitle=&rft.title=Research+communications+in+chemical+pathology+and+pharmacology&rft.issn=00345164&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-07 N1 - Date created - 1988-04-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Characterization of a hepatic proliferation inhibitor (HPI): effect of HPI on the growth of normal liver cells--comparison with transforming growth factor beta. AN - 77959694; 3126196 AB - Improvements in the purification of a hepatic proliferation inhibitor (HPI) from adult rat liver have yielded a product that has an inhibitory activity 1,000-fold greater than previously reported. The growth inhibitory activity, which could be eluted from SDS-PAGE at 17-19 kilodaltons (kD), was compared to that of transforming growth factor beta (TGF-beta). The ID50 of the HPI preparation in Fischer rat liver epithelial cells was 50 pg/ml (2.5 pM) compared to a value of 260 pg/ml (10.4 pM) obtained for pure human TGF-beta. Both inhibitors also modulated the stimulation of DNA synthesis in primary hepatocytes by either epidermal growth factor or a growth stimulatory activity prepared from serum of hepatectomized rats. The ID50s of HPI and TGF-beta in these cells were 250 pg/ml and 40 pg/ml, respectively. In contrast to TGF-beta the growth inhibitory activity of HPI was unaltered in the presence of an antibody raised against TGF-beta. The possible mechanism of action of HPI is discussed. JF - Journal of cellular biochemistry AU - Huggett, A C AU - Krutzsch, H C AU - Thorgeirsson, S S AD - Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 305 EP - 314 VL - 35 IS - 4 SN - 0730-2312, 0730-2312 KW - Growth Inhibitors KW - 0 KW - Peptides KW - Proteins KW - Transforming Growth Factors KW - 76057-06-2 KW - DNA KW - 9007-49-2 KW - Arginase KW - EC 3.5.3.1 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Electrophoresis, Polyacrylamide Gel KW - Cells, Cultured KW - Cell Division -- drug effects KW - DNA -- biosynthesis KW - Male KW - Proteins -- pharmacology KW - Liver -- cytology KW - Liver -- growth & development KW - Liver -- drug effects KW - Growth Inhibitors -- pharmacology KW - Peptides -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77959694?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cellular+biochemistry&rft.atitle=Characterization+of+a+hepatic+proliferation+inhibitor+%28HPI%29%3A+effect+of+HPI+on+the+growth+of+normal+liver+cells--comparison+with+transforming+growth+factor+beta.&rft.au=Huggett%2C+A+C%3BKrutzsch%2C+H+C%3BThorgeirsson%2C+S+S&rft.aulast=Huggett&rft.aufirst=A&rft.date=1987-12-01&rft.volume=35&rft.issue=4&rft.spage=305&rft.isbn=&rft.btitle=&rft.title=Journal+of+cellular+biochemistry&rft.issn=07302312&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-19 N1 - Date created - 1988-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Arabinosyl-5-azacytosine: a novel nucleoside entering clinical trials. AN - 77959214; 2449402 AB - Arabinosyl-5-azacytosine is a new compound which has been selected by the Division of Cancer Treatment, National Cancer Institute for clinical development as an antineoplastic agent based on its high degree of activity against a broad range of tumor types in preclinical studies. Therapeutic activity has been observed against murine and human leukemias, transplantable murine solid tumors, and human tumor xenografts. Arabinosyl-5-azacytosine exhibited a broader spectrum of activity against human solid tumors than cytosine arabinoside. Arabinosyl-5-azacytosine is phosphorylated to the nucleotide level by deoxycytidine kinase. Upon further anabolism to the triphosphate level, it can be incorporated into DNA. The mechanism of cytotoxicity is thought to be related to inhibition of DNA synthesis. Leukemic and solid tumor cell lines that are resistant to cytosine arabinoside due to deletion of deoxycytidine kinase activity are cross-resistant to arabinosyl-5-azacytosine. Unlike cytosine arabinoside, arabinosyl-5-azacytosine does not readily undergo deamination. Schedule dependence has been demonstrated in mice bearing L1210 leukemia, with superior activity seen with multiple doses administered on each treatment day compared to administration of larger but less frequently administered doses. From preliminary data in solid tumor models, however, antitumor activity did not appear to be superior with continuous infusion compared to that observed on a bolus schedule. Preclinical toxicology studies indicated that the bone marrow and gastrointestinal tract were the main target organs. A single large dose of arabinosyl-5-azacytosine could be tolerated by both mice and dogs. When administered as a continuous infusion, the toxicity was related to both the dose and duration of exposure, suggesting that toxicity resulted from a critical time above a threshold concentration as opposed to the total area under the concentration-time curve. Phase I clinical trials have been initiated to determine the maximum tolerated dose on a low dose continuous infusion schedule for 72 hours and also on a high dose short infusion daily times five schedule. JF - Investigational new drugs AU - Grem, J L AU - Shoemaker, D D AU - Hoth, D F AU - King, S A AU - Plowman, J AU - Zaharko, D AU - Grieshaber, C K AU - Harrison, S D AU - Cradock, J C AU - Leyland-Jones, B AD - Cancer Therapy Evaluation Program, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 315 EP - 328 VL - 5 IS - 4 SN - 0167-6997, 0167-6997 KW - Antiviral Agents KW - 0 KW - fazarabine KW - 5V71D8JOKK KW - Azacitidine KW - M801H13NRU KW - Index Medicus KW - Humans KW - Antiviral Agents -- therapeutic use KW - Azacitidine -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77959214?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Investigational+new+drugs&rft.atitle=Arabinosyl-5-azacytosine%3A+a+novel+nucleoside+entering+clinical+trials.&rft.au=Grem%2C+J+L%3BShoemaker%2C+D+D%3BHoth%2C+D+F%3BKing%2C+S+A%3BPlowman%2C+J%3BZaharko%2C+D%3BGrieshaber%2C+C+K%3BHarrison%2C+S+D%3BCradock%2C+J+C%3BLeyland-Jones%2C+B&rft.aulast=Grem&rft.aufirst=J&rft.date=1987-12-01&rft.volume=5&rft.issue=4&rft.spage=315&rft.isbn=&rft.btitle=&rft.title=Investigational+new+drugs&rft.issn=01676997&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-07 N1 - Date created - 1988-04-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Adrenocortical function in type II hyperlipoproteinemic patients treated with lovastatin (mevinolin). AN - 77957194; 2831127 AB - Lovastatin (Mevinolin), a competitive inhibitor of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase, has been used effectively as a hypocholesterolemic agent in man. As an inhibitor of endogenous cholesterol synthesis, a potentially serious side effect of therapy with this drug is interference with adrenocortical function. The effect of lovastatin on adrenal function was evaluated in a 6-month, randomized, double blinded, placebo-controlled, crossover study involving 24 type II hyperlipoproteinemic patients. Despite significant lowering of total and low density lipoprotein (LDL) cholesterol by lovastatin, no statistically or clinically significant differences were seen in free cortisol excretion or in plasma cortisol response to intravenous ACTH infusion between baseline, placebo, and lovastatin-treated patients. We conclude that lovastatin does not adversely affect adrenocortical reserve in patients with heterozygous familial hypercholesterolemia (FH) or non-FH type II hyperlipoproteinemia. JF - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme AU - Fojo, S S AU - Hoegg, J M AU - Lackner, K J AU - Anchors, J M AU - Bailey, K R AU - Brewer, H B AD - Molecular Disease Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 648 EP - 652 VL - 19 IS - 12 SN - 0018-5043, 0018-5043 KW - Adrenocorticotropic Hormone KW - 9002-60-2 KW - Lovastatin KW - 9LHU78OQFD KW - Hydrocortisone KW - WI4X0X7BPJ KW - Index Medicus KW - Humans KW - Heterozygote KW - Adrenocorticotropic Hormone -- pharmacology KW - Hydrocortisone -- blood KW - Lovastatin -- adverse effects KW - Hyperlipoproteinemia Type II -- blood KW - Hyperlipoproteinemia Type II -- physiopathology KW - Lovastatin -- therapeutic use KW - Hyperlipoproteinemia Type II -- drug therapy KW - Adrenal Cortex -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77957194?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Hormone+and+metabolic+research+%3D+Hormon-+und+Stoffwechselforschung+%3D+Hormones+et+metabolisme&rft.atitle=Adrenocortical+function+in+type+II+hyperlipoproteinemic+patients+treated+with+lovastatin+%28mevinolin%29.&rft.au=Fojo%2C+S+S%3BHoegg%2C+J+M%3BLackner%2C+K+J%3BAnchors%2C+J+M%3BBailey%2C+K+R%3BBrewer%2C+H+B&rft.aulast=Fojo&rft.aufirst=S&rft.date=1987-12-01&rft.volume=19&rft.issue=12&rft.spage=648&rft.isbn=&rft.btitle=&rft.title=Hormone+and+metabolic+research+%3D+Hormon-+und+Stoffwechselforschung+%3D+Hormones+et+metabolisme&rft.issn=00185043&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-14 N1 - Date created - 1988-04-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Induction of glucagon sensitivity in a transformed kidney cell line by prostaglandin E2 and its inhibition by epidermal growth factor. AN - 77945543; 2830489 AB - A model system using a transformed dog kidney cell line (Madin-Darby canine kidney), has been established for studying the process of differentiation. Glucagon responsiveness can be restored to these transformed cells by various differentiation inducers, including prostaglandin E2. Glucagon response was measured in terms of the ability of glucagon to stimulate cAMP production. Induction of glucagon sensitivity seems to be mediated by cAMP. The ability of various prostaglandin analogs to elevate the cAMP level correlates closely with their ability to induce glucagon sensitivity. In fact, 8-Br-cAMP is also a potent inducer. To define the nature of this cAMP-mediated process, we identified several inhibitors of this induction process. These differentiation inhibitors include serum, phorbol ester, and epidermal growth factor. These inhibitors do not have a direct effect on cAMP production by cells in the presence or absence of hormones. Furthermore, induction by 8-Br-cAMP is also inhibited by these agents. Therefore, the site of inhibition is located beyond the point of cAMP production. Possible interaction between cAMP- and epidermal growth factor-dependent phosphorylations is discussed. JF - Molecular and cellular biology AU - Lin, M C AU - Darfler, F J AU - Beckner, S K AD - Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 4324 EP - 4328 VL - 7 IS - 12 SN - 0270-7306, 0270-7306 KW - Prostaglandins KW - 0 KW - Prostaglandins E KW - 8-Bromo Cyclic Adenosine Monophosphate KW - 23583-48-4 KW - Epidermal Growth Factor KW - 62229-50-9 KW - Glucagon KW - 9007-92-5 KW - Cyclic AMP KW - E0399OZS9N KW - Dinoprostone KW - K7Q1JQR04M KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Blood KW - Animals KW - Kidney KW - Dogs KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Cell Line, Transformed KW - Cell Differentiation -- drug effects KW - Prostaglandins -- pharmacology KW - 8-Bromo Cyclic Adenosine Monophosphate -- pharmacology KW - Cyclic AMP -- biosynthesis KW - Prostaglandins E -- pharmacology KW - Glucagon -- pharmacology KW - Epidermal Growth Factor -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77945543?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biology&rft.atitle=Induction+of+glucagon+sensitivity+in+a+transformed+kidney+cell+line+by+prostaglandin+E2+and+its+inhibition+by+epidermal+growth+factor.&rft.au=Lin%2C+M+C%3BDarfler%2C+F+J%3BBeckner%2C+S+K&rft.aulast=Lin&rft.aufirst=M&rft.date=1987-12-01&rft.volume=7&rft.issue=12&rft.spage=4324&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biology&rft.issn=02707306&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-04-01 N1 - Date created - 1988-04-01 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1977 May 25;252(10):3554-7 [193837] Methods Enzymol. 1985;109:356-60 [2985924] Science. 1985 Jul 19;229(4710):267-9 [2990047] Proc Natl Acad Sci U S A. 1986 Jan;83(2):236-40 [3079907] Biochem Biophys Res Commun. 1977 Jun 20;76(4):1181-7 [302703] Nature. 1978 Nov 16;276(5685):274-5 [714157] Nature. 1979 May 24;279(5711):336-8 [221826] J Biol Chem. 1979 Sep 25;254(18):9317-23 [225326] Proc Natl Acad Sci U S A. 1979 Jul;76(7):3338-42 [291007] J Biol Chem. 1980 May 25;255(10):4834-42 [6246084] J Biol Chem. 1981 Feb 10;256(3):1396-403 [6256395] Endocrinology. 1982 Oct;111(4):1192-200 [6180889] Exp Cell Res. 1982 Nov;142(1):181-9 [6291963] Proc Natl Acad Sci U S A. 1983 Jan;80(1):36-40 [6296873] J Biol Chem. 1983 Mar 10;258(5):2789-94 [6298205] J Biol Chem. 1983 Jun 25;258(12):7386-94 [6134722] Nature. 1983 Dec 1-7;306(5942):487-90 [6196643] Exp Cell Res. 1984 May;152(1):31-7 [6325222] Mol Cell Endocrinol. 1984 Feb;34(2):113-9 [6325268] Methods Enzymol. 1985;109:360-5 [2985925] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Patch-clamp study of the calcium-dependent chloride current in AtT-20 pituitary cells. AN - 77940852; 2449518 AB - 1. Voltage-clamp recordings were made from cultured AtT-20 pituitary cells using the whole-cell patch-clamp technique. Cells were perfused internally with Cs+ to block K+ currents and bathed externally with either 1 microM tetrodotoxin or with tetraethylammonium (TEA) as a Na+ substitute to block voltage-activated Na+ currents. 2. Depolarizing voltage steps from a holding potential of -80 mV to potentials positive to -30 mV evoked two currents: a fast inward current that activated between -30 and +70 mV and a slowly activating current (designated "slow step current") that was inward between -30 and near 0 mV (the Cl- equilibrium potential) and outward positive to about 0 mV. Repolarization to -80 mV revealed a slowly decaying, inward tail current, whose magnitude with respect to step potential closely matched the current-voltage relationship of the voltage-activated Ca2+ current. 3. Activation of the fast inward current, slow step current, and tail current, was prevented by extracellular application of Cd2+ or removal of extracellular Ca2+. Replacement of extracellular Ca2+ with Ba2+ potentiated the fast inward current but blocked the slow step and tail currents. Intracellular perfusion with greater than 1 mM of the Ca2+ chelators ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA) or [1,2-bis(2)aminophenoxy]ethane N,N,N',N'-tetraacetic acid (BAPTA) prevented activation of the slow step and tail currents, but not the fast inward current. 4. The reversal potential of the slow inward current was sensitive to changes in the Cl- equilibrium potential but not to substitution of TEA for Na+. The slow step current, but not the fast inward current, was partially blocked by the Cl- channel blocker, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid. 5. These data indicate that both the slow inward tail current and the slowly activating, reversible step current were a Ca2+-dependent Cl- current, similar to that described in other neuronal and nonneuronal cell types. The fast inward current was a voltage-activated Ca2+ current, described previously in these and other cells. 6. In the absence of intracellular EGTA, the tail current decayed with complex kinetics, its time course apparently dependent on the magnitude of the voltage-activated Ca2+ current. In the presence of 200 microM intracellular EGTA, the tail current decayed significantly faster and often decayed exponentially. JF - Journal of neurophysiology AU - Korn, S J AU - Weight, F F AD - Section on Electrophysiology, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1431 EP - 1451 VL - 58 IS - 6 SN - 0022-3077, 0022-3077 KW - Chlorides KW - 0 KW - Ion Channels KW - Tetraethylammonium Compounds KW - Cesium KW - 1KSV9V4Y4I KW - Barium KW - 24GP945V5T KW - Tetrodotoxin KW - 4368-28-9 KW - Tetraethylammonium KW - 66-40-0 KW - Sodium KW - 9NEZ333N27 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Cesium -- pharmacology KW - Animals KW - Tetraethylammonium Compounds -- pharmacology KW - Chlorides -- metabolism KW - Pituitary Neoplasms KW - Sodium -- pharmacology KW - Kinetics KW - Membrane Potentials -- drug effects KW - Tetrodotoxin -- pharmacology KW - Barium -- pharmacology KW - Cell Line KW - Chlorides -- pharmacology KW - Ion Channels -- drug effects KW - Calcium -- pharmacology KW - Ion Channels -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77940852?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Patch-clamp+study+of+the+calcium-dependent+chloride+current+in+AtT-20+pituitary+cells.&rft.au=Korn%2C+S+J%3BWeight%2C+F+F&rft.aulast=Korn&rft.aufirst=S&rft.date=1987-12-01&rft.volume=58&rft.issue=6&rft.spage=1431&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-25 N1 - Date created - 1988-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Transfer of di(2-ethylhexyl) phthalate through rat milk and effects on milk composition and the mammary gland. AN - 77912004; 2892284 AB - Five daily oral doses of di(2-ethylhexyl) phthalate (DEHP) (2 g/kg) given to rats on Days 2-6, 6-10, or 14-18 of lactation caused significant decreases in body weight and increases in hepatic peroxisomal enzymes palmitoyl CoA oxidase and carnitine acetyltransferase in the dams and their suckling pups. Plasma cholesterol and triglyceride levels were decreased in the lactating dams. Decreased food consumption, as indicated by pair-fed rats, accounted for the decreased body weight in the pups but not the increases in enzyme activities. To determine whether DEHP and mono(2-ethylhexyl) phthalate (MEHP) were transferred through the milk, milk and plasma were collected from lactating rats 6 hr after the third dose of DEHP. The milk contained 216 +/- 23 micrograms/ml DEHP and 25 +/- 6 micrograms/ml MEHP (mean +/- SE), while the plasma contained less than 0.5 micrograms/ml DEHP and 75 +/- 12 micrograms/ml MEHP. The high milk/plasma ratio for DEHP (greater than 200) indicates efficient extraction of DEHP from the plasma into the milk. DEHP dosing during lactation also caused a decrease in mammary gland weight and a decrease in mammary gland RNA content which reflects synthetic activity. The water content of the milk was reduced, which probably accounted for the increase in lipid in the milk. Milk lactose was decreased in DEHP-treated and pair-fed rats, consistent with the decrease in milk production. The results show that exposure to high doses of DEHP during lactation in rats can result in changes in milk quality and quantity and can lead to DEHP and MEHP exposure in the suckling rat pups. JF - Toxicology and applied pharmacology AU - Dostal, L A AU - Weaver, R P AU - Schwetz, B A AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 315 EP - 325 VL - 91 IS - 3 SN - 0041-008X, 0041-008X KW - Lipids KW - 0 KW - Phthalic Acids KW - Diethylhexyl Phthalate KW - C42K0PH13C KW - Carnitine O-Acetyltransferase KW - EC 2.3.1.7 KW - Palmitoyl-CoA Hydrolase KW - EC 3.1.2.2 KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Lipids -- blood KW - Animals KW - Drug Administration Schedule KW - Animals, Suckling -- metabolism KW - Palmitoyl-CoA Hydrolase -- metabolism KW - Body Weight -- drug effects KW - Carnitine O-Acetyltransferase -- metabolism KW - Female KW - Pregnancy KW - Lactation -- drug effects KW - Milk -- metabolism KW - Mammary Glands, Animal -- drug effects KW - Milk -- drug effects KW - Diethylhexyl Phthalate -- toxicity KW - Diethylhexyl Phthalate -- pharmacokinetics KW - Diethylhexyl Phthalate -- analogs & derivatives KW - Phthalic Acids -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77912004?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Transfer+of+di%282-ethylhexyl%29+phthalate+through+rat+milk+and+effects+on+milk+composition+and+the+mammary+gland.&rft.au=Dostal%2C+L+A%3BWeaver%2C+R+P%3BSchwetz%2C+B+A&rft.aulast=Dostal&rft.aufirst=L&rft.date=1987-12-01&rft.volume=91&rft.issue=3&rft.spage=315&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=0041008X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-02 N1 - Date created - 1988-02-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Epidemiology of acute myelogenous leukemia. AN - 77898392; 3321440 AB - The epidemiologic literature suggests that environmental exposures, familial susceptibility, and cytogenetic changes affect AML risk in childhood and adulthood. Unfortunately, many studies are limited by inadequate sample sizes, imprecise case definition, or inadequate exposure measurement. Few studies have singled out AML alone, either because of insufficient numbers or because methods of case ascertainment made it difficult to distinguish specific cell types. Studies of total leukemia or all acute leukemias offer insights into potential risk factors for AML, but may also be misleading in instances when few AML patients were actually included. Future studies should include adequate numbers of patients with AML. At the same time, further refinement of case definition through parameters such as specific cytogenetic changes may make risk factor identification in epidemiologic studies more likely. JF - Seminars in oncology AU - Sandler, D P AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 359 EP - 364 VL - 14 IS - 4 SN - 0093-7754, 0093-7754 KW - Index Medicus KW - United States KW - Humans KW - Adult KW - Environmental Exposure KW - Child KW - Leukemia, Myeloid, Acute -- epidemiology KW - Leukemia, Myeloid, Acute -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77898392?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Seminars+in+oncology&rft.atitle=Epidemiology+of+acute+myelogenous+leukemia.&rft.au=Sandler%2C+D+P&rft.aulast=Sandler&rft.aufirst=D&rft.date=1987-12-01&rft.volume=14&rft.issue=4&rft.spage=359&rft.isbn=&rft.btitle=&rft.title=Seminars+in+oncology&rft.issn=00937754&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-25 N1 - Date created - 1988-01-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Organic cation secretion in flounder renal tissue. AN - 77894687; 2962510 AB - In the winter flounder, Pseudopleuronectes americanus, renal clearance experiments showed that the model organic cations, tetraethylammonium (TEA) and N'-methylnicotinamide (NMN), were strongly secreted; organic cation-to-polyethylene glycol (glomerular filtration rate marker) clearance ratios averaged 130 and 30, respectively. TEA uptake by isolated renal tubular masses was concentrative and saturable. Transport was inhibited by competitor organic cations and reduced by exposure to NaCN,2,4-dinitrophenol, ouabain, and HgCl2. Organic anions did not reduce TEA uptake. NMN was the poorest inhibitor of TEA uptake of all the organic cations tested. In addition, the rate of NMN uptake was slower than that of TEA, and the steady-state tissue-to-medium ratio was lower (5 for NMN vs. 10 for TEA; both at 25 microM). The data show the presence of an organic cation secretory system in flounder tissue that resembles the mammalian systems in several respects. JF - The American journal of physiology AU - Miller, D S AU - Holohan, P D AD - Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - R861 EP - R867 VL - 253 IS - 6 Pt 2 SN - 0002-9513, 0002-9513 KW - Cations KW - 0 KW - Dinitrophenols KW - Tetraethylammonium Compounds KW - Niacinamide KW - 25X51I8RD4 KW - Mercuric Chloride KW - 53GH7MZT1R KW - Ouabain KW - 5ACL011P69 KW - Sodium Cyanide KW - O5DDB9Z95G KW - 2,4-Dinitrophenol KW - Q13SKS21MN KW - N(1)-methylnicotinamide KW - UM47085BXC KW - Index Medicus KW - Niacinamide -- secretion KW - Sodium Cyanide -- pharmacology KW - Animals KW - Reference Values KW - Glomerular Filtration Rate KW - Niacinamide -- analogs & derivatives KW - Dinitrophenols -- pharmacology KW - Mercuric Chloride -- pharmacology KW - Biological Transport, Active KW - Ouabain -- pharmacology KW - Tetraethylammonium Compounds -- secretion KW - Kidney -- secretion KW - Flatfishes -- physiology KW - Flounder -- physiology KW - Cations -- secretion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77894687?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+physiology&rft.atitle=Organic+cation+secretion+in+flounder+renal+tissue.&rft.au=Miller%2C+D+S%3BHolohan%2C+P+D&rft.aulast=Miller&rft.aufirst=D&rft.date=1987-12-01&rft.volume=253&rft.issue=6+Pt+2&rft.spage=R861&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+physiology&rft.issn=00029513&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-28 N1 - Date created - 1988-01-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Bladder cancer, drinking water source, and tap water consumption: a case-control study. AN - 77891249; 3480378 AB - Data from a population-based case-control interview study of incident bladder cancer in 10 areas of the United States were used to estimate relative risks among white men (2,116 cases, 3,892 controls) and women (689 cases, 1,366 controls) according to beverage intake level and type of water source. Individual year-by-year profiles of water source and treatment were developed by linking lifetime residential information with historical water utility data from an ancillary survey. Risk of bladder cancer increased with intake level of beverages made with tap water. The odds ratio (OR) for the highest vs. lowest quintile of tap water consumption was 1.43 [95% confidence interval (CI) = 1.23, 1.67; chi 2 for trend = 26.3, P less than .001]. The risk gradient with intake was restricted to persons with at least a 40-year exposure to chlorinated surface water and was not found among long-term users of nonchlorinated ground water. The ORs for the highest vs. lowest quintiles of tap water intake were 1.7 and 2.0, respectively, among subjects with 40-59 and greater than or equal to 60 years' exposure. Duration of exposure to chlorinated surface water was associated with bladder cancer risk among women and nonsmokers of both sexes. Among non-smoking respondents with tap water consumption above the population median, the OR increased with exposure duration to a level of 3.1 (CI = 1.3, 7.3; chi 2 for trend = 6.3, P = .01) for greater than or equal to 60 years of residence at places served by chlorinated surface water (vs. non-chlorinated ground water users). These results extend findings of earlier epidemiologic studies and are consistent with environmental chemistry and toxicologic data demonstrating the presence of genotoxic by-products of chlorine disinfection in treated surface waters. JF - Journal of the National Cancer Institute AU - Cantor, K P AU - Hoover, R AU - Hartge, P AU - Mason, T J AU - Silverman, D T AU - Altman, R AU - Austin, D F AU - Child, M A AU - Key, C R AU - Marrett, L D AD - Environmental Epidemiology Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1269 EP - 1279 VL - 79 IS - 6 SN - 0027-8874, 0027-8874 KW - Chlorine KW - 4R7X1O2820 KW - Index Medicus KW - United States KW - Sex Factors KW - Risk Factors KW - Humans KW - Geography KW - Male KW - Female KW - Drinking KW - Urinary Bladder Neoplasms -- etiology KW - Urinary Bladder Neoplasms -- epidemiology KW - Water Supply UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77891249?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Bladder+cancer%2C+drinking+water+source%2C+and+tap+water+consumption%3A+a+case-control+study.&rft.au=Cantor%2C+K+P%3BHoover%2C+R%3BHartge%2C+P%3BMason%2C+T+J%3BSilverman%2C+D+T%3BAltman%2C+R%3BAustin%2C+D+F%3BChild%2C+M+A%3BKey%2C+C+R%3BMarrett%2C+L+D&rft.aulast=Cantor&rft.aufirst=K&rft.date=1987-12-01&rft.volume=79&rft.issue=6&rft.spage=1269&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-17 N1 - Date created - 1988-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - NIH conference. Alcohol withdrawal and noradrenergic function. AN - 77880919; 2825572 AB - Alcohol withdrawal syndrome is characterized by signs of overactivity of the sympathetic nervous system. Biochemical studies indicate that increased release of norepinephrine is associated with certain symptoms of alcohol withdrawal, and the severity of the withdrawal symptoms correlates positively with the amount of norepinephrine released. In the rat, the brain epinephrine concentration is reduced by alcohol, a phenomenon probably associated with both the intoxicating and rewarding effects of alcohol intake. Furthermore, intoxicating effects of alcohol can be reversed by inhibiting epinephrine synthesis in the rat brain. In this species, alcohol withdrawal is associated with profound depletion of epinephrine in the hypothalamus. When clonidine, a norepinephrine alpha-2-receptor agonist, was infused in alcoholics, these receptors were found to be subsensitive during alcohol withdrawal, and this subsensitivity may contribute to the syndrome. Repeated withdrawals may lead to "kindling" and thus further enhancement of noradrenergic overactivity. Pituitary responsiveness to corticotropin-releasing hormone, which is a central regulator of stress responses and increases the firing rate of brain noradrenergic neurons, is altered during alcohol withdrawal. JF - Annals of internal medicine AU - Linnoila, M AU - Mefford, I AU - Nutt, D AU - Adinoff, B AD - National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 875 EP - 889 VL - 107 IS - 6 SN - 0003-4819, 0003-4819 KW - Receptors, Adrenergic, alpha KW - 0 KW - Corticotropin-Releasing Hormone KW - 9015-71-8 KW - Clonidine KW - MN3L5RMN02 KW - Norepinephrine KW - X4W3ENH1CV KW - Epinephrine KW - YKH834O4BH KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Kindling, Neurologic KW - Hypothalamo-Hypophyseal System -- physiopathology KW - Humans KW - Brain Chemistry -- drug effects KW - Pituitary-Adrenal System -- physiopathology KW - Receptors, Adrenergic, alpha -- physiology KW - Epinephrine -- metabolism KW - Epinephrine -- physiology KW - Norepinephrine -- physiology KW - Substance Withdrawal Syndrome -- physiopathology KW - Substance Withdrawal Syndrome -- metabolism KW - Norepinephrine -- metabolism KW - Sympathetic Nervous System -- physiopathology KW - Alcoholism -- metabolism KW - Alcoholism -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77880919?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+internal+medicine&rft.atitle=NIH+conference.+Alcohol+withdrawal+and+noradrenergic+function.&rft.au=Linnoila%2C+M%3BMefford%2C+I%3BNutt%2C+D%3BAdinoff%2C+B&rft.aulast=Linnoila&rft.aufirst=M&rft.date=1987-12-01&rft.volume=107&rft.issue=6&rft.spage=875&rft.isbn=&rft.btitle=&rft.title=Annals+of+internal+medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-07 N1 - Date created - 1988-01-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Characterization of the lower respiratory tract inflammation of nonsmoking individuals with interstitial lung disease associated with chronic inhalation of inorganic dusts. AN - 77880621; 2825569 AB - The pneumoconioses, interstitial lung disorders resulting from the inhalation of inorganic dusts, are associated with chronic inflammatory processes in the lower respiratory tract. To characterize these inflammatory processes in relation to the pathogenesis of these disorders, we studied 39 nonsmoking individuals with long-term occupational exposures to inorganic dust and functional evidence of interstitial disease (asbestosis, n = 18; coal workers' pneumoconiosis, n = 15; silicosis, n = 6). In all 3 disorders, the inflammation was dominated by alveolar macrophages. Because a common feature of these interstitial lung diseases is concurrent injury and fibrosis of alveolar walls, we assessed whether these alveolar macrophages were spontaneously releasing mediators capable of giving rise to these changes. Alveolar macrophages from the study population were spontaneously releasing increased amounts of superoxide anion and hydrogen peroxide (both p less than 0.01 compared to normals), oxidants capable of injuring lung parenchymal cells. The alveolar macrophages were also spontaneously releasing significantly increased amounts of fibronectin and alveolar macrophage-derived growth factor (both p less than 0.01 compared to normals), mediators that act synergistically to signal fibroblast replication. Taken together, these findings define a major role for the alveolar macrophage in mediating the alveolar wall injury and fibrosis that characterize the common pneumoconioses and suggest that the alveolar macrophage is an important "target" for developing strategies designed to prevent loss of lung function in these individuals. JF - The American review of respiratory disease AU - Rom, W N AU - Bitterman, P B AU - Rennard, S I AU - Cantin, A AU - Crystal, R G AD - Pulmonary Branch. National Heart, Lung, and Blood Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1429 EP - 1434 VL - 136 IS - 6 SN - 0003-0805, 0003-0805 KW - Fibronectins KW - 0 KW - Gallium Radioisotopes KW - Growth Substances KW - Peptides KW - alveolar macrophage growth factor KW - Superoxides KW - 11062-77-4 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Abridged Index Medicus KW - Index Medicus KW - Silicosis -- metabolism KW - Silicosis -- diagnostic imaging KW - Lung -- diagnostic imaging KW - Humans KW - Hydrogen Peroxide -- metabolism KW - Fibronectins -- metabolism KW - Asbestosis -- metabolism KW - Growth Substances -- metabolism KW - Asbestosis -- etiology KW - Radionuclide Imaging KW - Asbestosis -- diagnostic imaging KW - Bronchoalveolar Lavage Fluid -- metabolism KW - Superoxides -- metabolism KW - Pulmonary Alveoli -- metabolism KW - Adult KW - Middle Aged KW - Bronchoalveolar Lavage Fluid -- cytology KW - Silicosis -- etiology KW - Male KW - Female KW - Macrophages -- metabolism KW - Smoking KW - Pneumoconiosis -- diagnostic imaging KW - Pulmonary Fibrosis -- etiology KW - Pulmonary Fibrosis -- diagnostic imaging KW - Pneumoconiosis -- etiology KW - Pneumoconiosis -- metabolism KW - Pulmonary Fibrosis -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77880621?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+review+of+respiratory+disease&rft.atitle=Characterization+of+the+lower+respiratory+tract+inflammation+of+nonsmoking+individuals+with+interstitial+lung+disease+associated+with+chronic+inhalation+of+inorganic+dusts.&rft.au=Rom%2C+W+N%3BBitterman%2C+P+B%3BRennard%2C+S+I%3BCantin%2C+A%3BCrystal%2C+R+G&rft.aulast=Rom&rft.aufirst=W&rft.date=1987-12-01&rft.volume=136&rft.issue=6&rft.spage=1429&rft.isbn=&rft.btitle=&rft.title=The+American+review+of+respiratory+disease&rft.issn=00030805&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-05 N1 - Date created - 1988-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Role of transforming growth factor-beta in the development of the mouse embryo. AN - 77879474; 3320058 AB - Using immunohistochemical methods, we have investigated the role of transforming growth factor-beta (TGF-beta) in the development of the mouse embryo. For detection of TGF-beta in 11-18-d-old embryos, we have used a polyclonal antibody specific for TGF-beta type 1 and the peroxidase-antiperoxidase technique. Staining of TGF-beta is closely associated with mesenchyme per se or with tissues derived from mesenchyme, such as connective tissue, cartilage, and bone. TGF-beta is conspicuous in tissues derived from neural crest mesenchyme, such as the palate, larynx, facial mesenchyme, nasal sinuses, meninges, and teeth. Staining of all of these tissues is greatest during periods of morphogenesis. In many instances, intense staining is seen in mesenchyme when critical interactions with adjacent epithelium occur, as in the development of hair follicles, teeth, and the submandibular gland. Marked staining is also seen when remodeling of mesenchyme or mesoderm occurs, as during formation of digits from limb buds, formation of the palate, and formation of the heart valves. The presence of TGF-beta is often coupled with pronounced angiogenic activity. The histochemical results are discussed in terms of the known biochemical actions of TGF-beta, especially its ability to control both synthesis and degradation of both structural and adhesion molecules of the extracellular matrix. JF - The Journal of cell biology AU - Heine, U AU - Munoz, E F AU - Flanders, K C AU - Ellingsworth, L R AU - Lam, H Y AU - Thompson, N L AU - Roberts, A B AU - Sporn, M B AD - Laboratory of Comparative Carcinogenesis and Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 2861 EP - 2876 VL - 105 IS - 6 Pt 2 SN - 0021-9525, 0021-9525 KW - Fixatives KW - 0 KW - Peptides KW - RNA, Messenger KW - Transforming Growth Factors KW - 76057-06-2 KW - Index Medicus KW - Animals KW - RNA, Messenger -- metabolism KW - Connective Tissue -- metabolism KW - Meninges -- metabolism KW - Heart -- embryology KW - Mesoderm -- metabolism KW - Bone and Bones -- metabolism KW - Myocardium -- metabolism KW - Immunoenzyme Techniques KW - Meninges -- embryology KW - Bone and Bones -- embryology KW - Mice -- embryology KW - Peptides -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77879474?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+cell+biology&rft.atitle=Role+of+transforming+growth+factor-beta+in+the+development+of+the+mouse+embryo.&rft.au=Heine%2C+U%3BMunoz%2C+E+F%3BFlanders%2C+K+C%3BEllingsworth%2C+L+R%3BLam%2C+H+Y%3BThompson%2C+N+L%3BRoberts%2C+A+B%3BSporn%2C+M+B&rft.aulast=Heine&rft.aufirst=U&rft.date=1987-12-01&rft.volume=105&rft.issue=6+Pt+2&rft.spage=2861&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+cell+biology&rft.issn=00219525&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-20 N1 - Date created - 1988-02-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Natl Cancer Inst Monogr. 1967 Sep;26:279-99 [4864107] Mol Cell Biol. 1985 Dec;5(12):3644-6 [3870134] Eur J Biochem. 1980 Jun;107(2):303-14 [6772444] Cell Tissue Res. 1980;211(2):269-91 [6998561] Science. 1981 Jul 31;213(4507):563-5 [7017936] Ann N Y Acad Sci. 1981 Feb 27;359:200-17 [6266308] EMBO J. 1986 Aug;5(8):1831-7 [3758028] Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10 [3022285] J Biol Chem. 1986 Dec 15;261(35):16509-13 [3465726] J Cell Biol. 1986 Nov;103(5):1799-805 [3465734] J Cell Biol. 1986 Dec;103(6 Pt 1):2403-10 [3491081] J Exp Med. 1987 Jan 1;165(1):251-6 [3491869] Nature. 1987 Jan 1-7;325(6099):81-4 [3467201] Cell. 1987 Feb 13;48(3):409-15 [2879635] Cell Biol Int Rep. 1986 Dec;10(12):915-22 [3467860] J Biol Chem. 1987 Feb 15;262(5):1946-9 [3469199] J Biol Chem. 1987 Feb 25;262(6):2869-74 [3469200] J Biol Chem. 1987 Mar 15;262(8):3897-902 [3493244] Nucleic Acids Res. 1987 Apr 10;15(7):3187 [3470708] Proc Natl Acad Sci U S A. 1987 Apr;84(7):2024-8 [3494250] Cell. 1987 May 22;49(4):437-8 [3471351] J Biol Chem. 1987 May 15;262(14):6443-6 [3471760] J Cell Biol. 1987 May;104(5):1361-74 [3553211] Nature. 1987 Jun 18-24;327(6123):625-8 [3600758] Biochemistry. 1987 May 5;26(9):2406-10 [3475130] EMBO J. 1987 May;6(5):1281-6 [3111844] EMBO J. 1987 Jun;6(6):1633-6 [3497030] J Cell Biol. 1987 Jul;105(1):457-63 [3475276] J Cell Biol. 1987 Jul;105(1):473-82 [2440898] Proc Natl Acad Sci U S A. 1987 Aug;84(16):5788-92 [2886992] EMBO J. 1987 Jul;6(7):1899-904 [2820711] J Cell Biol. 1987 Sep;105(3):1039-45 [3308901] Cell. 1987 Oct 23;51(2):189-97 [3499229] Mol Endocrinol. 1987 Oct;1(10):693-8 [3153459] Cell. 1981 Oct;26(1 Pt 1):99-105 [7034958] J Cell Physiol. 1982 Feb;110(2):169-74 [6279682] Dev Biol. 1982 Aug;92(2):315-29 [7117690] Science. 1983 Mar 18;219(4590):1329-31 [6572416] J Cell Biol. 1983 Feb;96(2):462-73 [6833366] Cancer Res. 1983 Jul;43(7):3034-40 [6189589] J Biol Chem. 1983 Jun 10;258(11):7155-60 [6602130] J Cell Biol. 1983 Jul;97(1):153-65 [6190818] Cell. 1984 Jan;36(1):35-41 [6319010] Dev Biol. 1984 Feb;101(2):336-45 [6363163] Cancer Res. 1984 Apr;44(4):1635-41 [6322983] Proc Natl Acad Sci U S A. 1985 Apr;82(8):2267-71 [3857579] Nature. 1985 Aug 22-28;316(6030):701-5 [3861940] Adv Cancer Res. 1985;44:139-266 [2930999] J Histochem Cytochem. 1986 Jan;34(1):85-91 [2416801] J Biol Chem. 1986 Mar 25;261(9):4337-45 [3456347] J Biol Chem. 1986 Apr 5;261(10):4377-9 [3007454] J Biol Chem. 1986 May 5;261(13):5693-5 [3754555] J Cell Biol. 1986 May;102(5):1917-30 [2422181] J Embryol Exp Morphol. 1985 Dec;90:437-55 [3834038] Proc Natl Acad Sci U S A. 1986 Jun;83(12):4167-71 [2424019] Science. 1986 Aug 1;233(4763):532-4 [3487831] J Biol Chem. 1986 Aug 15;261(23):10478-81 [3488314] Biochem Biophys Res Commun. 1986 Jul 31;138(2):974-80 [3461787] J Biol Chem. 1986 Sep 15;261(26):12362-7 [3528157] Cell. 1976 Oct;9(2):231-40 [975245] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Neuroleptic responsivity of negative and positive symptoms in schizophrenia. AN - 77874369; 3688278 AB - The authors prospectively examined the effects of double-blind, placebo-controlled neuroleptic withdrawal and administration on ratings of negative and positive symptoms in 19 young patients with chronic schizophrenia. Negative symptoms were significantly reduced by neuroleptic treatment, and negative and positive symptoms demonstrated similar patterns of reduction and exacerbation during neuroleptic treatment and withdrawal, respectively. The changes in negative and positive symptoms induced by neuroleptic treatment and withdrawal were not significantly correlated, however. The negative and positive symptom profiles of individual patients were significantly altered by neuroleptic treatment, indicating limitations to the cross-sectional classification of patients on the basis of predominance of one or the other symptom group. The authors discuss implications for the neurobiological underpinnings of negative and positive symptoms. JF - The American journal of psychiatry AU - Breier, A AU - Wolkowitz, O M AU - Doran, A R AU - Roy, A AU - Boronow, J AU - Hommer, D W AU - Pickar, D AD - Section on Clinical Studies, NIMH, Bethesda, Md. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1549 EP - 1555 VL - 144 IS - 12 SN - 0002-953X, 0002-953X KW - Placebos KW - 0 KW - Fluphenazine KW - S79426A41Z KW - Dopamine KW - VTD58H1Z2X KW - Abridged Index Medicus KW - Index Medicus KW - Double-Blind Method KW - Humans KW - Dopamine -- physiology KW - Brain -- physiopathology KW - Prospective Studies KW - Psychiatric Status Rating Scales KW - Substance Withdrawal Syndrome -- etiology KW - Adult KW - Substance Withdrawal Syndrome -- psychology KW - Chronic Disease KW - Female KW - Male KW - Fluphenazine -- adverse effects KW - Schizophrenia -- diagnosis KW - Fluphenazine -- therapeutic use KW - Schizophrenic Psychology KW - Schizophrenia -- drug therapy KW - Fluphenazine -- administration & dosage KW - Schizophrenia -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77874369?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Neuroleptic+responsivity+of+negative+and+positive+symptoms+in+schizophrenia.&rft.au=Breier%2C+A%3BWolkowitz%2C+O+M%3BDoran%2C+A+R%3BRoy%2C+A%3BBoronow%2C+J%3BHommer%2C+D+W%3BPickar%2C+D&rft.aulast=Breier&rft.aufirst=A&rft.date=1987-12-01&rft.volume=144&rft.issue=12&rft.spage=1549&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-30 N1 - Date created - 1987-12-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Obsessive-compulsive symptoms in panic disorder. AN - 77873249; 3688281 AB - Previous reports have noted an increased prevalence of obsessive-compulsive symptoms in patients with panic disorder. The authors found a prevalence of obsessive-compulsive symptoms in 19 (27%) of 70 patients with panic disorder. Compared to a subgroup of 25 patients with classic features of panic disorder and no obsessive-compulsive symptoms, the subgroup with obsessive-compulsive symptoms had an earlier onset of illness, were more likely to have personal and family histories of major depression and substance abuse, and showed a poorer outcome after treatment. JF - The American journal of psychiatry AU - Mellman, T A AU - Uhde, T W AD - Unit on Anxiety and Affective Disorders, NIMH, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1573 EP - 1576 VL - 144 IS - 12 SN - 0002-953X, 0002-953X KW - Abridged Index Medicus KW - Index Medicus KW - Substance-Related Disorders -- diagnosis KW - Psychiatric Status Rating Scales KW - Alcoholism -- diagnosis KW - Humans KW - Adult KW - Outcome and Process Assessment (Health Care) KW - Depressive Disorder -- diagnosis KW - Depressive Disorder -- genetics KW - Alcoholism -- genetics KW - Substance-Related Disorders -- genetics KW - Male KW - Female KW - Obsessive-Compulsive Disorder -- diagnosis KW - Fear KW - Anxiety Disorders -- psychology KW - Obsessive-Compulsive Disorder -- psychology KW - Obsessive-Compulsive Disorder -- complications KW - Anxiety Disorders -- diagnosis KW - Panic KW - Anxiety Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77873249?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Obsessive-compulsive+symptoms+in+panic+disorder.&rft.au=Mellman%2C+T+A%3BUhde%2C+T+W&rft.aulast=Mellman&rft.aufirst=T&rft.date=1987-12-01&rft.volume=144&rft.issue=12&rft.spage=1573&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-30 N1 - Date created - 1987-12-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Radiation dose and leukemia risk in patients treated for cancer of the cervix. AN - 77870107; 3480381 AB - To quantify the risk of radiation-induced leukemia and provide further information on the nature of the relationship between dose and response, a case-control study was undertaken in a cohort of over 150,000 women with invasive cancer of the uterine cervix. The cases either were reported to one of 17 population-based cancer registries or were treated in any of 16 oncologic clinics in Canada, Europe, and the United States. Four controls were individually matched to each of 195 cases of leukemia on the basis of age and calendar year when diagnosed with cervical cancer and survival time. Leukemia diagnoses were verified by one hematologist. Radiation dose to active bone marrow was estimated by medical physicists on the basis of the original radiotherapy records of study subjects. The risk of chronic lymphocytic leukemia, one of the few malignancies without evidence for an association with ionizing radiation, was not increased [relative risk (RR) = 1.03; n = 52]. However, for all other forms of leukemia taken together (n = 143), a twofold risk was evident (RR = 2.0; 90% confidence interval = 1.0-4.2). Risk increased with increasing radiation dose until average doses of about 400 rad (4 Gy) were reached and then decreased at higher doses. This pattern is consistent with experimental data for which the down-turn in risk at high doses has been interpreted as due to killing of potentially leukemic cells. The dose-response information was modeled with various RR functions, accounting for the nonhomogeneous distribution of radiation dose during radiotherapy. The local radiation doses to each of 14 bone marrow compartments for each patient were incorporated in the models, and the corresponding risks were summed. A good fit to the observed data was obtained with a linear-exponential function, which included a positive linear induction term and a negative exponential term. The estimate of the excess RR per rad was 0.9%, and the estimated RR at 100 rad (1 Gy) was 1.7. The model proposed in this study of risk proportional to mass exposed and of risk to an individual given by the sum of incremental risks to anatomic sites appears to be applicable to a wide range of dose distributions. Furthermore, the pattern of leukemia incidence associated with different levels of radiation dose is consistent with a model postulating increasing risk with increasing exposure, modified at high doses by increased frequency of cell death, which reduces risk. JF - Journal of the National Cancer Institute AU - Boice, J D AU - Blettner, M AU - Kleinerman, R A AU - Stovall, M AU - Moloney, W C AU - Engholm, G AU - Austin, D F AU - Bosch, A AU - Cookfair, D L AU - Krementz, E T AU - Latourette, H B AU - Peters, L J AU - Schulz, M D AU - Lundell, M AU - Pettersson, F AU - Storm, H H AU - Bell, C M AU - Coleman, M P AU - Fraser, P AU - Palmer, M AU - Prior, P AU - Choi, N W AU - Hislop, T G AU - Koch, M AU - Robb, D AU - Robson, D AU - Spengler, R F AU - von Fournier, D AU - Frischkorn, R AU - Lochmüller, H AU - Pompe-Kirn, V AU - Rimpela, A AU - Kjørstad, K AU - Pejovic, M H AU - Sigurdsson, K AU - Pisani, P AU - Kucera, H AU - Hutchison, G B AD - Radiation Epidemiology Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1295 EP - 1311 VL - 79 IS - 6 SN - 0027-8874, 0027-8874 KW - Index Medicus KW - United States KW - Age Factors KW - Brachytherapy -- adverse effects KW - Humans KW - Aged KW - Europe KW - Registries KW - Radiotherapy Dosage KW - Risk Factors KW - Adult KW - Middle Aged KW - Bone Marrow -- radiation effects KW - Female KW - Leukemia, Radiation-Induced -- etiology KW - Uterine Cervical Neoplasms -- radiotherapy KW - Radiotherapy -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77870107?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Radiation+dose+and+leukemia+risk+in+patients+treated+for+cancer+of+the+cervix.&rft.au=Boice%2C+J+D%3BBlettner%2C+M%3BKleinerman%2C+R+A%3BStovall%2C+M%3BMoloney%2C+W+C%3BEngholm%2C+G%3BAustin%2C+D+F%3BBosch%2C+A%3BCookfair%2C+D+L%3BKrementz%2C+E+T%3BLatourette%2C+H+B%3BPeters%2C+L+J%3BSchulz%2C+M+D%3BLundell%2C+M%3BPettersson%2C+F%3BStorm%2C+H+H%3BBell%2C+C+M%3BColeman%2C+M+P%3BFraser%2C+P%3BPalmer%2C+M%3BPrior%2C+P%3BChoi%2C+N+W%3BHislop%2C+T+G%3BKoch%2C+M%3BRobb%2C+D%3BRobson%2C+D%3BSpengler%2C+R+F%3Bvon+Fournier%2C+D%3BFrischkorn%2C+R%3BLochm%C3%BCller%2C+H%3BPompe-Kirn%2C+V%3BRimpela%2C+A%3BKj%C3%B8rstad%2C+K%3BPejovic%2C+M+H%3BSigurdsson%2C+K%3BPisani%2C+P%3BKucera%2C+H%3BHutchison%2C+G+B&rft.aulast=Boice&rft.aufirst=J&rft.date=1987-12-01&rft.volume=79&rft.issue=6&rft.spage=1295&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-17 N1 - Date created - 1988-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The neostriatal mosaic: III. Biochemical and developmental dissociation of patch-matrix mesostriatal systems. AN - 77866567; 2891800 AB - In the previous paper (Gerfen et al., 1987) mesostriatal dopaminergic neurons were shown to be subdivided into dorsal and ventral tiers that project to the striatal matrix and patch compartments, respectively. The present study provides experimental evidence that these patch-matrix mesostriatal dopaminergic systems are biochemically and developmentally distinct. A 28 kDa calcium-binding protein (CaBP, or calbindin-D28 kDa) is expressed in dorsal tier mesostriatal dopaminergic neurons. The distribution of such neurons, located in the ventral tegmental area, dorsal tier of the substantia nigra pars compacta, and retrorubral area, matches that of dopaminergic neurons that project to the striatal matrix. Dopaminergic neurons that do not express CaBP--those in the ventral tier of the pars compacta and in the pars reticulata--are distributed in a pattern that matches the origin of the dopaminergic projection to the striatal patches. During development, dopaminergic afferents to the striatal patch compartment are in place prior to the development of those to the matrix. Injections of the neurotoxin 6-hydroxydopamine (6-OHDA) into the striatum of newborn rats result in a selective and long-lasting depletion of dopaminergic afferents in the striatal patches. The later-developing matrix projection is relatively spared by such lesions. The distribution of surviving dopaminergic neurons, labeled with tyrosine hydroxylase (TH) immunoreactivity, matches the pattern of dorsal tier neurons previously shown to provide inputs to the matrix. Surviving neurons also express CaBP immunoreactivity and have dendrites that spread mediolaterally, in the plane of the pars compacta. On the other hand, those neurons that project to the patches are selectively lesioned by the neonatal 6-OHDA striatal injections, do not express CaBP, and have dendrites that are directed ventrally into the pars reticulata. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Gerfen, C R AU - Baimbridge, K G AU - Thibault, J AD - Laboratory of Neurophysiology, National Institute of Mental Health, Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 3935 EP - 3944 VL - 7 IS - 12 SN - 0270-6474, 0270-6474 KW - Calbindins KW - 0 KW - Hydroxydopamines KW - S100 Calcium Binding Protein G KW - Oxidopamine KW - 8HW4YBZ748 KW - Tyrosine 3-Monooxygenase KW - EC 1.14.16.2 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Tyrosine 3-Monooxygenase -- analysis KW - Animals KW - Neural Pathways -- analysis KW - Brain Diseases -- physiopathology KW - Neural Pathways -- cytology KW - Corpus Striatum -- cytology KW - Mesencephalon -- analysis KW - Dopamine -- analysis KW - Mesencephalon -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77866567?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=The+neostriatal+mosaic%3A+III.+Biochemical+and+developmental+dissociation+of+patch-matrix+mesostriatal+systems.&rft.au=Gerfen%2C+C+R%3BBaimbridge%2C+K+G%3BThibault%2C+J&rft.aulast=Gerfen&rft.aufirst=C&rft.date=1987-12-01&rft.volume=7&rft.issue=12&rft.spage=3935&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-28 N1 - Date created - 1988-01-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - A prospective randomized trial of regional versus systemic continuous 5-fluorodeoxyuridine chemotherapy in the treatment of colorectal liver metastases. AN - 77866507; 2961314 AB - Sixty-four patients were entered into a randomized trial that evaluated intra-arterial (I.A.) versus intravenous (I.V.) 5-fluorodeoxyuridine (FUDR) for colorectal liver metastases. There was a significant improved response rate for I.A. (62%) compared with I.V. (17%) treatment (p less than 0.003). However, the improved response rate for patients in whom I.A. therapy was used did not translate to a significantly improved survival rate. The 2-year actuarial survival rates for the groups for which I.A. and I.V. therapy was used were 22% and 15% respectively, with the survival curves not differing significantly (p = 0.27). These results may have been due to the inclusion of patients with tumor in draining hepatic lymph nodes. The presence of tumor in hepatic lymph nodes was associated with a poorer prognosis. Analysis of a subgroup of patients with negative hepatic lymph nodes suggested an improved actuarial survival rate in patients for whom I.A. versus I.V. therapy was used (p less than 0.03). The toxicity of I.A. FUDR was considerable, and side effects included chemical hepatitis (79%), biliary sclerosis (21%), peptic ulcers (17%), and gastritis/duodenitis (21%). The only major effect of toxicity of I.V. FUDR was severe diarrhea (59%). Regional I.A. FUDR allowed more drug delivery to liver tumors, which resulted in increased tumor responses when compared with use of systemic therapy. However, the small gain in survival seen in a select subgroup of patients with negative hepatic nodes appeared to be offset by the toxicity of I.A. FUDR. JF - Annals of surgery AU - Chang, A E AU - Schneider, P D AU - Sugarbaker, P H AU - Simpson, C AU - Culnane, M AU - Steinberg, S M AD - Surgery Branch, National Cancer Institute, Bethesda, Maryland. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 685 EP - 693 VL - 206 IS - 6 SN - 0003-4932, 0003-4932 KW - Floxuridine KW - 039LU44I5M KW - Abridged Index Medicus KW - Index Medicus KW - Prospective Studies KW - Infusions, Intravenous KW - Random Allocation KW - Lymphatic Metastasis KW - Humans KW - Adult KW - Tomography, X-Ray Computed KW - Prognosis KW - Aged KW - Middle Aged KW - Male KW - Female KW - Floxuridine -- administration & dosage KW - Liver Neoplasms -- pathology KW - Infusions, Intra-Arterial KW - Rectal Neoplasms KW - Liver Neoplasms -- drug therapy KW - Liver Neoplasms -- secondary KW - Colonic Neoplasms KW - Floxuridine -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77866507?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+surgery&rft.atitle=A+prospective+randomized+trial+of+regional+versus+systemic+continuous+5-fluorodeoxyuridine+chemotherapy+in+the+treatment+of+colorectal+liver+metastases.&rft.au=Chang%2C+A+E%3BSchneider%2C+P+D%3BSugarbaker%2C+P+H%3BSimpson%2C+C%3BCulnane%2C+M%3BSteinberg%2C+S+M&rft.aulast=Chang&rft.aufirst=A&rft.date=1987-12-01&rft.volume=206&rft.issue=6&rft.spage=685&rft.isbn=&rft.btitle=&rft.title=Annals+of+surgery&rft.issn=00034932&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-04 N1 - Date created - 1988-01-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer Chemother Rep. 1966 Mar;50(3):163-70 [5910392] N Engl J Med. 1964 Feb 13;270:321-7 [14082260] Surg Gynecol Obstet. 1972 Jan;134(1):51-6 [5007175] Cancer Res. 1978 Nov;38(11 Pt 1):3784-92 [151583] Surgery. 1979 Oct;86(4):550-5 [384574] Cancer. 1980 Mar 1;45(5):866-9 [7260838] Cancer. 1982 Sep 1;50(5):850-62 [6212114] Am J Surg. 1983 Apr;145(4):529-33 [6220618] Semin Oncol. 1983 Jun;10(2):135-47 [6306833] Ann Surg. 1983 Nov;198(5):567-73 [6227295] Cancer. 1984 Mar 15;53(6):1336-43 [6229326] Cancer Invest. 1983;1(3):237-57 [6365269] J Clin Oncol. 1983 May;1(5):337-44 [6199474] Ann Surg. 1984 Mar;199(3):317-24 [6703793] J Clin Oncol. 1984 May;2(5):498-504 [6547166] J Clin Oncol. 1984 Jun;2(6):595-600 [6233400] J Clin Oncol. 1985 Jan;3(1):98-102 [3155548] J Clin Oncol. 1985 Feb;3(2):161-9 [3155793] Cancer. 1985 Apr 1;55(7):1490-4 [3978541] Ann Surg. 1985 Aug;202(2):176-81 [3160313] Int J Radiat Oncol Biol Phys. 1985 Nov;11(11):1941-6 [2997090] Recent Results Cancer Res. 1986;100:171-8 [2942993] J Clin Oncol. 1986 Sep;4(9):1356-64 [2943876] Surgery. 1987 Jul;102(1):79-87 [3589978] Am J Pathol. 1954 Sep-Oct;30(5):969-77 [13197542] Surgery. 1970 Aug;68(2):334-40 [5450714] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Cytogenetic and environmental factors in the etiology of the acute leukemias in adults. AN - 77858242; 3318409 JF - American journal of epidemiology AU - Sandler, D P AU - Collman, G W AD - Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1017 EP - 1032 VL - 126 IS - 6 SN - 0002-9262, 0002-9262 KW - Index Medicus KW - Acute Disease KW - Risk Factors KW - Humans KW - Adult KW - Environmental Exposure KW - Chromosome Aberrations KW - Leukemia -- epidemiology KW - Leukemia -- etiology KW - Leukemia -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77858242?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+epidemiology&rft.atitle=Cytogenetic+and+environmental+factors+in+the+etiology+of+the+acute+leukemias+in+adults.&rft.au=Sandler%2C+D+P%3BCollman%2C+G+W&rft.aulast=Sandler&rft.aufirst=D&rft.date=1987-12-01&rft.volume=126&rft.issue=6&rft.spage=1017&rft.isbn=&rft.btitle=&rft.title=American+journal+of+epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-07 N1 - Date created - 1988-01-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Heterogeneity of intercellular adhesion in rat liver cells in culture. AN - 77853150; 3691677 AB - The intercellular homotypic adhesive properties of 14 clones derived from a nontumorigenic rat liver epithelial cell line (LEC), derived from neonatal Fischer rats, were examined and compared to those of the hepatoma H4-II-E cell line. Each clone was assayed also for the degree of chromosomal aneuploidy and the ability to grow in soft agar. Over 100-fold differences in adhesive properties were observed among the clones, but no correlation was observed between the degree of aneuploidy in the clones and intercellular adhesive properties. The parent LEC cell line and the clones derived from it were unable to grow in soft agar. The H4-II-E cells showed negligible capacity to reaggregate after dissociation into single cells and these cells readily formed colonies in soft agar. Many of the LEC clones were similar to the H4-II-E cells in their adhesive properties, which suggests that reduced cell-to-cell adhesiveness per se is not a necessary prerequisite of epithelial cells to be able to grow independent of anchorage. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of concanavalin A (Con A)-binding glycoproteins in the "most adhesive" clone 67 and the "least adhesive" clone 201 showed markedly elevated amounts of acidic 105 and 67-kDa glycoproteins in clone 67. Proteins with similar migration patterns in 2D-PAGE have previously been reported to participate in specific homotypic intercellular adhesion of liver cells. The Con A-binding glycoprotein pattern in H4-II-E cells was markedly different from that of LEC cells with a set of six proteins missing and nine proteins appearing new in the H4-II-E cells. It is suggested that, in addition to identifying known epithelial cell polypeptides, systematic screening of cell surface-associated glycoproteins in normal and transformed epithelial cells in vitro and in vivo may lead to identification of novel polypeptides intimately associated with the transformed phenotype. JF - Experimental cell research AU - Raunio, H AU - Konno, R AU - Linnainmaa, K AU - Wirth, P J AU - Thorgeirsson, S S AD - Laboratory of Experimental Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 596 EP - 605 VL - 173 IS - 2 SN - 0014-4827, 0014-4827 KW - Glycoproteins KW - 0 KW - Receptors, Concanavalin A KW - concanavalin A-binding glycoproteins KW - Index Medicus KW - Animals KW - Receptors, Concanavalin A -- classification KW - Aneuploidy KW - Glycoproteins -- analysis KW - Electrophoresis, Polyacrylamide Gel KW - Glycoproteins -- classification KW - Clone Cells -- classification KW - Rats KW - Rats, Inbred F344 KW - Chromosomes KW - Clone Cells -- physiology KW - Receptors, Concanavalin A -- analysis KW - Cell Aggregation KW - Cell Line KW - Male KW - Cell Division KW - Liver -- cytology KW - Cell Communication KW - Cell Adhesion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77853150?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+cell+research&rft.atitle=Heterogeneity+of+intercellular+adhesion+in+rat+liver+cells+in+culture.&rft.au=Raunio%2C+H%3BKonno%2C+R%3BLinnainmaa%2C+K%3BWirth%2C+P+J%3BThorgeirsson%2C+S+S&rft.aulast=Raunio&rft.aufirst=H&rft.date=1987-12-01&rft.volume=173&rft.issue=2&rft.spage=596&rft.isbn=&rft.btitle=&rft.title=Experimental+cell+research&rft.issn=00144827&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-02 N1 - Date created - 1988-02-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Idiopathic dilated cardiomyopathy: analysis of 152 necropsy patients. AN - 77851571; 3687784 AB - Certain clinical and cardiac necropsy findings are described in 152 patients aged 16 to 78 years (mean 45) with idiopathic dilated cardiomyopathy: 109 (72%) were men and 43 (28%) were women. Compared with the women, the men had a significantly (p less than 0.05) shorter mean duration of chronic congestive heart failure (CHF) (43 vs 69 months), a higher percentage of habitual alcoholism (40 vs 24%) and a higher mean heart weight (632 vs 551 g). The male to female ratio among the 58 known alcoholics was 7.3:1 and among the 70 known nonalcoholics, 1.5:1 (p less than 0.05). The mean duration of clinical evidence of CHF was similar among the known alcoholics and the known non-alcoholics (each 50 months). Of the 152 patients, 148 (97%) had clinical evidence of chronic CHF; in 114 patients it was the initial manifestation of idiopathic dilated cardiomyopathy, and in most it became intractable and caused death. The interval from onset of chronic CHF to death (known in 120 patients) ranged from 1 to 264 months (mean 54). Comparison of the 27 patients surviving greater than 72 months after onset of chronic CHF to the 64 patients surviving less than or equal to 36 months disclosed a significantly higher frequency in the longer survival group of older patients, of women, of habitual alcoholics, of patients with chest pain syndromes, diabetes mellitus, pulmonary emboli, of patients treated with warfarin and of patients with larger hearts at necropsy. Each of the 4 patients without chronic CHF died suddenly and sudden death was the initial manifestation of idiopathic dilated cardiomyopathy in them. An additional 33 patients also died suddenly, but each of them previously had had chronic CHF. Of the 79 patients (of the 131 for whom information was available) with either pulmonary or systemic emboli or both, 67 (85%) had either right- or left-sided thrombi or mural endocardial plaques or both, whereas of the 52 patients without emboli, 36 (69%) had intracardiac thrombi or plaques (p less than (0.05). Electrocardiograms in the last 6 months of life in 101 patients disclosed atrial fibrillation in 25; complete left (41 patients) or right (6 patients) bundle branch block or indeterminate intraventricular conduction delay (4 patients) in 51 patients; QRS voltage indicative of ventricular hypertrophy in 44 patients (left ventricular in 39 patients.(ABSTRACT TRUNCATED AT 400 WORDS) JF - The American journal of cardiology AU - Roberts, W C AU - Siegel, R J AU - McManus, B M AD - Pathology Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892. Y1 - 1987/12/01/ PY - 1987 DA - 1987 Dec 01 SP - 1340 EP - 1355 VL - 60 IS - 16 SN - 0002-9149, 0002-9149 KW - Abridged Index Medicus KW - Index Medicus KW - Heart Failure -- etiology KW - Pregnancy Complications KW - Embolism -- complications KW - Myocardium -- pathology KW - Humans KW - Hemodynamics KW - Aged KW - Heart Diseases -- complications KW - Organ Size KW - Pregnancy KW - Death, Sudden -- etiology KW - Electrocardiography KW - Adult KW - Heart Diseases -- genetics KW - Middle Aged KW - Adolescent KW - Alcoholism -- complications KW - Male KW - Diabetes Complications KW - Female KW - Cardiomyopathy, Dilated -- physiopathology KW - Cardiomyopathy, Dilated -- complications KW - Cardiomyopathy, Dilated -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77851571?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+cardiology&rft.atitle=Idiopathic+dilated+cardiomyopathy%3A+analysis+of+152+necropsy+patients.&rft.au=Roberts%2C+W+C%3BSiegel%2C+R+J%3BMcManus%2C+B+M&rft.aulast=Roberts&rft.aufirst=W&rft.date=1987-12-01&rft.volume=60&rft.issue=16&rft.spage=1340&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+cardiology&rft.issn=00029149&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-20 N1 - Date created - 1988-01-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Overview of current status and future direction of clinical trials with 5-fluorouracil in combination with folinic acid. AN - 77850570; 3319136 JF - Cancer treatment reports AU - Grem, J L AU - Hoth, D F AU - Hamilton, J M AU - King, S A AU - Leyland-Jones, B AD - Investigational Drug Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1249 EP - 1264 VL - 71 IS - 12 SN - 0361-5960, 0361-5960 KW - Leucovorin KW - Q573I9DVLP KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Rectal Neoplasms -- drug therapy KW - Humans KW - Colonic Neoplasms -- drug therapy KW - Clinical Trials as Topic KW - Forecasting KW - Fluorouracil -- administration & dosage KW - Fluorouracil -- adverse effects KW - Leucovorin -- administration & dosage KW - Leucovorin -- adverse effects KW - Leucovorin -- pharmacology KW - Antineoplastic Combined Chemotherapy Protocols -- administration & dosage KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Leucovorin -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77850570?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+treatment+reports&rft.atitle=Overview+of+current+status+and+future+direction+of+clinical+trials+with+5-fluorouracil+in+combination+with+folinic+acid.&rft.au=Grem%2C+J+L%3BHoth%2C+D+F%3BHamilton%2C+J+M%3BKing%2C+S+A%3BLeyland-Jones%2C+B&rft.aulast=Grem&rft.aufirst=J&rft.date=1987-12-01&rft.volume=71&rft.issue=12&rft.spage=1249&rft.isbn=&rft.btitle=&rft.title=Cancer+treatment+reports&rft.issn=03615960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-03 N1 - Date created - 1988-02-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Toxicological studies of chemical mixtures of environmental concern at the National Toxicology Program: health effects of groundwater contaminants. AN - 77849026; 3686528 AB - In cooperation with the Agency for Toxic Substances and Disease Registry, the National Toxicology Program is participating in a Public Health Service activity related to the Comprehensive Environmental Response, Compensation and Liability Act (Superfund Act) by conducting toxicology studies on chemicals found in high-priority hazardous waste sites and for which adequate toxicological data are not available. As part of this effort, a project on the toxicology of chemical mixtures of groundwater contaminants was initiated. The first study, centered on the health effects of groundwater contaminants, is at the contractual stage. Nineteen organic and six inorganic chemicals, selected from more than 1000 known groundwater contaminants, will be given in drinking water to Fischer 344 rats and B6C3F1 mice for 3 or 6 months. Controls and five dose levels, based on average concentrations (i.e., baseline level) of individual component chemicals, or 0.1-, 10-, or 1000-fold of the baseline level, will be used. Toxicological end points include mortality, clinical signs, water and food consumption, body and organ weights, clinical pathology analytes (e.g., hematology, clinical chemistry, and urinalysis), gross and histopathology, neurobehavioral tests, sperm morphology and vaginal cytology evaluations (SMVCE), and cytogenetics. This paper summarizes the rationale behind our experimental design and the factors one must consider when designing studies of complex chemical mixtures. JF - Toxicology AU - Yang, R S AU - Rauckman, E J AD - National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/12/01/ PY - 1987 DA - 1987 Dec 01 SP - 15 EP - 34 VL - 47 IS - 1-2 SN - 0300-483X, 0300-483X KW - Water Pollutants KW - 0 KW - Water Pollutants, Chemical KW - Index Medicus KW - United States KW - Rats KW - Mice, Inbred Strains KW - Animals KW - Rats, Inbred F344 KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Humans KW - Nervous System -- drug effects KW - Water Supply KW - Mice KW - Male KW - Female KW - Water Pollutants -- adverse effects KW - Water Pollutants, Chemical -- adverse effects KW - National Institutes of Health (U.S.) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77849026?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=Toxicological+studies+of+chemical+mixtures+of+environmental+concern+at+the+National+Toxicology+Program%3A+health+effects+of+groundwater+contaminants.&rft.au=Yang%2C+R+S%3BRauckman%2C+E+J&rft.aulast=Yang&rft.aufirst=R&rft.date=1987-12-01&rft.volume=47&rft.issue=1-2&rft.spage=15&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-12 N1 - Date created - 1988-01-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Treatment of pelvic sarcomas in adolescents and young adults with intensive combined modality therapy. AN - 77834903; 3679916 AB - Adolescent and young adult patients with pelvic sarcomas continue to have a poor prognosis with standard combination chemotherapy and local irradiation. In addition to a significant risk of local failure, these patients are at high risk for systemic relapse. Twenty-three consecutive patients with Ewing's sarcoma, alveolar rhabdomyosarcoma, undifferentiated sarcoma, or malignant peripheral neuroepithelioma originating in the pelvis were treated with short, intensive combined modality therapy. This approach integrates 5 cycles of VADRIAC chemotherapy (Vincristine, Adriamycin, Cyclophosphamide) with high dose irradiation to the primary lesion (55-60 Gy) and sites of gross metastatic disease (45-50 Gy). Following achievement of a complete response, intensification therapy consisting of total body irradiation (TBI) (8.0 Gy), high dose VADRIAC chemotherapy, and autologous bone marow transplantation is given. All therapy is completed within 6-7 months. No maintenance chemotherapy is given; no surgery is intended. Of the twenty-three patients with pelvic sarcomas treated on this combined modality protocol, 22 achieved a complete remission. Local control was achieved and maintained in all twenty-three patients. With a median follow-up of 21 months since initiation of treatment, there have been nine relapses (all systemic). Seven relapses occurred among the thirteen patients who presented with overt metastatic disease and the other two relapses were among the ten patients with localized disease at presentation. All seven metastatic patients who relapsed have died, whereas both of the relapsed localized patients remain alive. Acute and late toxicities have been acceptable using this aggressive combined modality approach. Induction chemotherapy had a significant impact on reduction of the typically large (greater than 10 cm diameter) soft tissue mass associated with these pelvic tumors, thus facilitating achievement of local control by high dose irradiation. Of 18 patients with measureable soft tissue tumor, all experienced a partial response (greater than 50% reduction in size) following the initial two cycles of chemotherapy given prior to local irradiation. In conclusion, this short, intensive chemoradiotherapeutic regimen is highly effective in controlling the primary lesion (100% local control) and inducing a complete response in a high proportion (96%) of these high risk pediatric and young adult patients with pelvic sarcomas. The role of TBI as "systemic" adjuvant therapy to control micrometastatic disease is discussed as still under investigation. JF - International journal of radiation oncology, biology, physics AU - Stea, B AU - Kinsella, T J AU - Triche, T J AU - Horvath, K AU - Glatstein, E AU - Miser, J S AD - Radiation Oncology, Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1797 EP - 1805 VL - 13 IS - 12 SN - 0360-3016, 0360-3016 KW - Index Medicus KW - Combined Modality Therapy KW - Humans KW - Adult KW - Neoplasm Metastasis KW - Adolescent KW - Sarcoma, Ewing -- therapy KW - Rhabdomyosarcoma -- therapy KW - Male KW - Female KW - Radiotherapy -- adverse effects KW - Sarcoma -- radiotherapy KW - Pelvic Neoplasms -- therapy KW - Antineoplastic Combined Chemotherapy Protocols -- administration & dosage KW - Sarcoma -- drug therapy KW - Pelvic Neoplasms -- radiotherapy KW - Pelvic Neoplasms -- drug therapy KW - Sarcoma -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77834903?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.atitle=Treatment+of+pelvic+sarcomas+in+adolescents+and+young+adults+with+intensive+combined+modality+therapy.&rft.au=Stea%2C+B%3BKinsella%2C+T+J%3BTriche%2C+T+J%3BHorvath%2C+K%3BGlatstein%2C+E%3BMiser%2C+J+S&rft.aulast=Stea&rft.aufirst=B&rft.date=1987-12-01&rft.volume=13&rft.issue=12&rft.spage=1797&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.issn=03603016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-12 N1 - Date created - 1988-01-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Testicular tumors in mice exposed in utero to diethylstilbestrol. AN - 77818321; 3682076 AB - Treatment of pregnant women with diethylstilbestrol (DES) is associated with the subsequent development of reproductive tract abnormalities such as epididymal cysts, retained hypotrophic testes and sperm abnormalities in their male offspring. It recently has been suggested that prenatal DES exposure is associated with development of testicular seminoma in humans. Studies of in utero exposure of laboratory animals to DES are few, but previous reports from our laboratory have described several abnormalities in the reproductive tract of the mouse following prenatal DES exposure. To study the possible association of testicular tumors and prenatal DES exposure in mice, pregnant outbred CD-1 mice were injected subcutaneously with daily doses of DES (100 micrograms./kg.) on days nine through 16 of gestation. DES-exposed and age-matched control male mice were sacrificed at 10 to 18 months of age and examined for testicular lesions. In addition to the nonmalignant abnormalities reported in previous studies such as 91% cryptorchidism and degenerative changes, interstitial cell tumors were observed in nine mice among 277 mice treated prenatally with DES. Two of these lesions were benign tumors and five were interstitial cell carcinomas. Rete testis adenocarcinoma was seen also in 5% of these DES-treated animals and is described in another report. The overall incidence of testicular tumors is 8% in DES-exposed male mice. No comparable lesions were seen in 122 control male mice. These results suggest that the testicular lesions that can occur following prenatal DES exposure include neoplasia. The combined prevalence of DES-induced tumors of the corpus testis and rete testis in mice suggests the male offspring may be more at risk for developing carcinoma of the reproductive tract than the female offspring. JF - The Journal of urology AU - Newbold, R R AU - Bullock, B C AU - McLachlan, J A AD - Developmental Endocrinology and Pharmacology Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1446 EP - 1450 VL - 138 IS - 6 SN - 0022-5347, 0022-5347 KW - Diethylstilbestrol KW - 731DCA35BT KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Testis -- drug effects KW - Testis -- pathology KW - Mice KW - Time Factors KW - Male KW - Female KW - Pregnancy KW - Testicular Neoplasms -- pathology KW - Diethylstilbestrol -- toxicity KW - Leydig Cell Tumor -- pathology KW - Testicular Neoplasms -- chemically induced KW - Leydig Cell Tumor -- chemically induced KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77818321?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+urology&rft.atitle=Testicular+tumors+in+mice+exposed+in+utero+to+diethylstilbestrol.&rft.au=Newbold%2C+R+R%3BBullock%2C+B+C%3BMcLachlan%2C+J+A&rft.aulast=Newbold&rft.aufirst=R&rft.date=1987-12-01&rft.volume=138&rft.issue=6&rft.spage=1446&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+urology&rft.issn=00225347&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-30 N1 - Date created - 1987-12-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Abnormal ultraviolet mutagenic spectrum in plasmid DNA replicated in cultured fibroblasts from a patient with the skin cancer-prone disease, xeroderma pigmentosum. AN - 77812541; 3680516 AB - A shuttle vector plasmid, pZ189, was utilized to assess the types of mutations that cells from a patient with xeroderma pigmentosum, complementation group D, introduce into ultraviolet (UV) damaged, replicating DNA. Patients with xeroderma pigmentosum have clinical and cellular UV hypersensitivity, increased frequency of sun-induced skin cancer, and deficient DNA repair. In comparison to UV-treated pZ189 replicated in DNA repair-proficient cells, there were fewer surviving plasmids, a higher frequency of plasmids with mutations, fewer plasmids with two or more mutations in the marker gene, and a new mutagenic hotspot. The major type of base substitution mutation was the G:C to A:T transition with both cell lines. These results, together with similar findings published earlier with cells from a xeroderma pigmentosum patient in complementation group A, suggest that isolated G:C to A:T somatic mutations may be particularly important in generation of human skin cancer by UV radiation. JF - The Journal of clinical investigation AU - Seetharam, S AU - Protić-Sabljić, M AU - Seidman, M M AU - Kraemer, K H AD - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 1613 EP - 1617 VL - 80 IS - 6 SN - 0021-9738, 0021-9738 KW - DNA KW - 9007-49-2 KW - Abridged Index Medicus KW - Index Medicus KW - DNA Damage KW - Humans KW - Radiation Genetics KW - Female KW - Cell Line KW - Ultraviolet Rays KW - Xeroderma Pigmentosum -- genetics KW - Plasmids -- radiation effects KW - DNA -- radiation effects KW - Mutation KW - DNA Replication UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77812541?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+clinical+investigation&rft.atitle=Abnormal+ultraviolet+mutagenic+spectrum+in+plasmid+DNA+replicated+in+cultured+fibroblasts+from+a+patient+with+the+skin+cancer-prone+disease%2C+xeroderma+pigmentosum.&rft.au=Seetharam%2C+S%3BProti%C4%87-Sablji%C4%87%2C+M%3BSeidman%2C+M+M%3BKraemer%2C+K+H&rft.aulast=Seetharam&rft.aufirst=S&rft.date=1987-12-01&rft.volume=80&rft.issue=6&rft.spage=1613&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+clinical+investigation&rft.issn=00219738&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-12 N1 - Date created - 1988-01-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Ann Intern Med. 1974 Feb;80(2):221-48 [4811796] J Mol Biol. 1964 Aug;9:372-5 [14202273] Proc Natl Acad Sci U S A. 1979 Aug;76(8):3982-6 [291058] Cell. 1982 Jun;29(2):451-8 [7116446] Biochimie. 1982 Aug-Sep;64(8-9):829-38 [6215955] Proc Natl Acad Sci U S A. 1983 Jan;80(2):487-91 [6300848] Carcinogenesis. 1984 Apr;5(4):511-4 [6705149] J Cell Physiol Suppl. 1984;3:45-62 [6378938] Lancet. 1984 Nov 17;2(8412):1138-42 [6150191] Proc Natl Acad Sci U S A. 1985 Oct;82(19):6622-6 [2995975] Clin Dermatol. 1985 Jan-Mar;3(1):33-69 [3833325] Biochemistry. 1986 May 6;25(9):2656-64 [3521740] Proc Natl Acad Sci U S A. 1986 Nov;83(21):8273-7 [3464953] Mol Cell Biol. 1986 Jan;6(1):277-85 [3537686] Mutat Res. 1986 Nov;166(3):287-94 [3023995] Mol Cell Biol. 1986 Oct;6(10):3349-56 [3540589] Arch Dermatol. 1987 Feb;123(2):241-50 [3545087] Proc Natl Acad Sci U S A. 1987 Jun;84(11):3782-6 [3473483] Proc Natl Acad Sci U S A. 1987 Jun;84(11):3787-91 [3108878] Proc Natl Acad Sci U S A. 1987 Jul;84(14):4944-8 [3474635] Mutat Res. 1979 Feb;59(2):273-83 [375075] N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Inhibition of NK and ADCC activity by antibodies against purified cytoplasmic granules from rat LGL tumors. AN - 77811097; 3316462 AB - Highly purified preparations of cytoplasmic granules from transplantable rat large granular lymphocyte (LGL) tumor lines (rat natural killer (RNK) tumors) were used to immunize rabbits. Antibodies from these animals gave two precipitin lines with granule extracts in Ouchterlony experiments. They reacted with at least four different bands on nitrocellulose blots of SDS gels of LGL granule proteins. By immunofluorescence, specifically adsorbed antigranule antibodies did not recognize LGL or T cell surface antigens but reacted with the cytoplasmic granules in permeabilized RNK tumor cells as well as with normal rat LGL. These same antisera showed little or no reactivity with a panel of other cells, including peripheral blood T cells, thymocytes, macrophages, and EL-4 tumor cells. F(ab')2 preparations of these antigranule antibodies completely blocked granule-mediated lysis of both SRBC and nucleated targets, while control F(ab')2 preparations from rabbits immunized with EL-4 granules or TNP-KLH showed no significant inhibition of this cytolytic activity at the same antibody concentration. Anti-granule F(ab')2 preparations specifically inhibited (greater than 75%) rat natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) activities in a dose-dependent manner but did not effect cytotoxic T cell activity. Pretreatment of either effectors or targets by these antibodies had no effect. Anti-granule F(ab')2 preparations, at concentrations showing strong inhibition of lysis, did not inhibit the binding of LGL to YAC-1 or Ab-coated P815 targets. These results demonstrate that a granule component(s) is necessary for the lytic activity of LGL in both NK and ADCC and provide the first direct evidence that a secretory event involving these granules is part of the lytic process. JF - Journal of leukocyte biology AU - Reynolds, C W AU - Reichardt, D AU - Henkart, M AU - Millard, P AU - Henkart, P AD - Cellular and Molecular Immunology Section, National Cancer Institute, Frederick Cancer Research Facility, Maryland. Y1 - 1987/12// PY - 1987 DA - December 1987 SP - 642 EP - 652 VL - 42 IS - 6 SN - 0741-5400, 0741-5400 KW - Antibodies KW - 0 KW - Immunoglobulin Fab Fragments KW - Neoplasm Proteins KW - Index Medicus KW - Rats KW - Animals KW - Neoplasm Proteins -- isolation & purification KW - Neoplasm Proteins -- immunology KW - Immunoglobulin Fab Fragments -- immunology KW - Cytotoxicity Tests, Immunologic KW - T-Lymphocytes, Cytotoxic -- immunology KW - Rabbits KW - Fluorescent Antibody Technique KW - Antibodies -- immunology KW - Lymphocytes -- immunology KW - Antibody-Dependent Cell Cytotoxicity KW - Cytoplasmic Granules -- immunology KW - Lymphocytes -- ultrastructure KW - Neoplasms, Experimental -- pathology KW - Killer Cells, Natural -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77811097?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+leukocyte+biology&rft.atitle=Inhibition+of+NK+and+ADCC+activity+by+antibodies+against+purified+cytoplasmic+granules+from+rat+LGL+tumors.&rft.au=Reynolds%2C+C+W%3BReichardt%2C+D%3BHenkart%2C+M%3BMillard%2C+P%3BHenkart%2C+P&rft.aulast=Reynolds&rft.aufirst=C&rft.date=1987-12-01&rft.volume=42&rft.issue=6&rft.spage=642&rft.isbn=&rft.btitle=&rft.title=Journal+of+leukocyte+biology&rft.issn=07415400&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-04 N1 - Date created - 1988-01-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - A novel cytotoxic T lymphocyte activation assay. Optimized conditions for antigen receptor triggered granule enzyme secretion. AN - 77844441; 3500234 AB - A method is described for the quantitative studies of cytotoxic T lymphocyte (CTL) activation. This functional assay is based on the measurements of secreted granule-associated enzymatic activity (BLT esterase (BLT-E) ) after incubation of CTL with activating stimuli. Immobilized mAb against CTL's antigen receptor (anti-TcR mAb), concanavalin A or a combination of PMA and ionophore A23187, were able to trigger the secretion of enzyme in the absence of target cells. Soluble anti-TcR mAb alone did not activate CTL, but using their conjugate with immobilized rabbit anti-mouse Ig antibody (RAMIg) TcR-mediated secretion of BLT-E was detected. Use of non-ionic detergents Nonidet P-40 or Triton X-100 (0.0125-0.2%) did not affect measurements of BLT-E activity. The efficiency of CTL exocytosis triggering by anti-TcR mAb which were immobilized on the surface of different plasticware is compared and conditions for studies of small and large numbers of CTL are described. The intensity of CTL response varies markedly with changes in buffer system, culture medium, additions of proteins. The optimal conditions for TcR complex triggered activation of murine CTL are described. Intensity of secretion can be easily manipulated by changing the surface density of immobilized anti-TcR mAb, thereby providing the possibility to screen inhibiting or activating agents (drugs or mAb) at selected sub-optimal levels of CTL activation. The potential for the use of described assay in screening of hybridoma supernatants for the presence of activating or inhibitory mAb against CTL's surface proteins is discussed. Since BLT-E secretion reflects exocytosis of granules from CTL, the conditions described here could be used for the detection of secretion of other markers of granules in future modifications of granule exocytosis assay. JF - Journal of immunological methods AU - Takayama, H AU - Trenn, G AU - Sitkovsky, M V AD - Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. Y1 - 1987/11/23/ PY - 1987 DA - 1987 Nov 23 SP - 183 EP - 190 VL - 104 IS - 1-2 SN - 0022-1759, 0022-1759 KW - Antibodies, Monoclonal KW - 0 KW - Detergents KW - Plastics KW - Receptors, Antigen, T-Cell KW - Concanavalin A KW - 11028-71-0 KW - Esterases KW - EC 3.1.- KW - Granzymes KW - EC 3.4.21.- KW - Serine Endopeptidases KW - Index Medicus KW - Lymphocyte Activation KW - Cytoplasmic Granules -- enzymology KW - Animals KW - Kinetics KW - Exocytosis KW - Mice KW - Concanavalin A -- pharmacology KW - Cell Line KW - Cytotoxicity Tests, Immunologic -- methods KW - Serine Endopeptidases -- genetics KW - T-Lymphocytes, Cytotoxic -- immunology KW - Receptors, Antigen, T-Cell -- immunology KW - Esterases -- secretion KW - T-Lymphocytes, Cytotoxic -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77844441?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunological+methods&rft.atitle=A+novel+cytotoxic+T+lymphocyte+activation+assay.+Optimized+conditions+for+antigen+receptor+triggered+granule+enzyme+secretion.&rft.au=Takayama%2C+H%3BTrenn%2C+G%3BSitkovsky%2C+M+V&rft.aulast=Takayama&rft.aufirst=H&rft.date=1987-11-23&rft.volume=104&rft.issue=1-2&rft.spage=183&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunological+methods&rft.issn=00221759&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-04 N1 - Date created - 1988-01-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Mechanisms of ultraviolet-induced mutation. Mutational spectra in the Escherichia coli lacI gene for a wild-type and an excision-repair-deficient strain. AN - 77915662; 2828636 AB - We have analyzed the DNA sequence changes in a total of 409 ultraviolet light-induced mutations in the lacI gene of Escherichia coli: 227 in a Uvr+ and 182 in a UvrB- strain. Both differences and similarities were observed. In both strains the mutations were predominantly (60 to 75%) base substitutions, followed by smaller contributions of single-base frameshifts, deletions and frameshift hotspot mutations. The base substitutions proved largely similar in the two strains but differences were observed among the single-base frameshifts, the deletions and the hotspot mutations. Among the base substitutions, both transitions (72.5%) and transversions (27.5%) were observed. The largest single group was G.C----A.T (60% of all base substitutions). The sites where G.C----A.T changes occurred were strongly correlated (97.5%) with sequences of adjacent pyrimidines, indicating mutation targeted ultraviolet photoproducts. Comparable amounts of mutation occurred at cytosine/cytosine and (mixed) cytosine/thymine sites. From an analysis of the prevalence of mutation at either the 5' or 3' side of a dipyrimidine, we conclude that both cyclobutane dimers and (6-4) lesions may contribute to mutation. Despite the general similarity of the base-substitution spectra between the wild-type and excision-defective strains, a number of sites were uniquely mutable in the UvrB- strain. Analysis of their surrounding DNA sequences suggested that, in addition to damage directly at the site of mutation, the potential for nearby opposite-strand damage may be important in determining the mutability of a site. The ultraviolet light-induced frameshift mutations were largely single-base losses. Inspection of the DNA sequences at which the frameshifts occurred suggested that they resulted from targeted mutagenesis, probably at cyclobutane pyrimidine dimers. The prevalence of frameshift mutations at homodimers (TT or CC) suggests that their formation involves local misalignment (slippage) and that base-pairing properties are partially retained in cyclobutane dimers. While the frameshift mutations in the Uvr+ strain were distributed over many different sites, more than half in the UvrB- strain were concentrated at a single site. Ultraviolet light-induced deletions as well as frameshift hotspot mutations (+/- TGGC at positions 620 to 632) are considered to be examples of untargeted or semitargeted mutagenesis. Hotspot mutations in the Uvr+ strain showed an increased contribution by (-)TGGC relative to (+)TGGC, indicating that ultraviolet light may specifically promote the loss of the four bases.(ABSTRACT TRUNCATED AT 400 WORDS) JF - Journal of molecular biology AU - Schaaper, R M AU - Dunn, R L AU - Glickman, B W AD - Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/11/20/ PY - 1987 DA - 1987 Nov 20 SP - 187 EP - 202 VL - 198 IS - 2 SN - 0022-2836, 0022-2836 KW - DNA Transposable Elements KW - 0 KW - DNA, Bacterial KW - Index Medicus KW - Base Sequence -- radiation effects KW - DNA Repair KW - DNA, Bacterial -- radiation effects KW - Ultraviolet Rays KW - Genes, Bacterial -- radiation effects KW - Escherichia coli -- genetics KW - Mutation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77915662?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+molecular+biology&rft.atitle=Mechanisms+of+ultraviolet-induced+mutation.+Mutational+spectra+in+the+Escherichia+coli+lacI+gene+for+a+wild-type+and+an+excision-repair-deficient+strain.&rft.au=Schaaper%2C+R+M%3BDunn%2C+R+L%3BGlickman%2C+B+W&rft.aulast=Schaaper&rft.aufirst=R&rft.date=1987-11-20&rft.volume=198&rft.issue=2&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Journal+of+molecular+biology&rft.issn=00222836&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-03-02 N1 - Date created - 1988-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of treatment on fertility in long-term survivors of childhood or adolescent cancer. AN - 77837749; 3683460 AB - In a retrospective cohort study of survivors of cancer and of controls, we estimated the risk of infertility after treatment for cancer during childhood or adolescence. We interviewed 2283 long-term survivors of childhood or adolescent cancer diagnosed in the period from 1945 through 1975, who were identified at five cancer centers in the United States. Requirements for admission to the study were diagnosis before the age of 20, survival for at least five years, and attainment of the age of 21. In addition, 3270 controls selected from among the survivors' siblings were interviewed. Cox regression analysis showed that cancer survivors who married and were presumed to be at risk of pregnancy were less likely than their sibling controls to have ever begun a pregnancy (relative fertility, 0.85; 95 percent confidence interval, 0.78 to 0.92). Radiation therapy directed below the diaphragm depressed fertility in both sexes by about 25 percent. Chemotherapy with alkylating agents, with or without radiation to sites below the diaphragm, was associated with a fertility deficit of about 60 percent in the men. Among the women, there was no apparent effect of alkylating-agent therapy administered alone (relative fertility, 1.02) and only a moderate fertility deficit when alkylating-agent therapy was combined with radiation below the diaphragm (relative fertility, 0.81). Relative fertility in the survivors varied considerably according to sex, site of cancer, and type of treatment; these factors should be taken into consideration in counseling survivors about the long-term consequences of disease. JF - The New England journal of medicine AU - Byrne, J AU - Mulvihill, J J AU - Myers, M H AU - Connelly, R R AU - Naughton, M D AU - Krauss, M R AU - Steinhorn, S C AU - Hassinger, D D AU - Austin, D F AU - Bragg, K AD - Clinical Epidemiology Branch, National Cancer Institute, Bethesda, MD 20892. Y1 - 1987/11/19/ PY - 1987 DA - 1987 Nov 19 SP - 1315 EP - 1321 VL - 317 IS - 21 SN - 0028-4793, 0028-4793 KW - Alkylating Agents KW - 0 KW - Antineoplastic Agents KW - Abridged Index Medicus KW - Index Medicus KW - Population KW - Fertility KW - Age Factors KW - Research Methodology KW - Population Dynamics KW - Physiology KW - Retrospective Studies KW - Child KW - Urogenital Effects KW - Treatment KW - Infertility--men KW - Infertility--women KW - Population Characteristics KW - Adult KW - Demographic Factors KW - Data Collection KW - Diseases KW - Data Analysis KW - Time Factors KW - Youth KW - Adolescents KW - Studies KW - Longterm Effects KW - Cancer KW - Neoplasms KW - Urogenital System KW - Reproduction KW - Biology KW - Regression Analysis KW - Infertility, Male -- etiology KW - Humans KW - Infertility, Female -- etiology KW - Combined Modality Therapy -- adverse effects KW - Adolescent KW - Male KW - Female KW - Pregnancy KW - Fertility -- radiation effects KW - Neoplasms -- therapy KW - Alkylating Agents -- adverse effects KW - Radiotherapy -- adverse effects KW - Fertility -- drug effects KW - Antineoplastic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77837749?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Effects+of+treatment+on+fertility+in+long-term+survivors+of+childhood+or+adolescent+cancer.&rft.au=Byrne%2C+J%3BMulvihill%2C+J+J%3BMyers%2C+M+H%3BConnelly%2C+R+R%3BNaughton%2C+M+D%3BKrauss%2C+M+R%3BSteinhorn%2C+S+C%3BHassinger%2C+D+D%3BAustin%2C+D+F%3BBragg%2C+K&rft.aulast=Byrne&rft.aufirst=J&rft.date=1987-11-19&rft.volume=317&rft.issue=21&rft.spage=1315&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-18 N1 - Date created - 1987-12-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Oxytocin and cholecystokinin induce grooming behavior in the ventral tegmentum of the rat. AN - 77852558; 3690307 AB - Oxytocin (OXY) and cholecystokinin (CCK) coexist in neurons of the supraoptic nucleus and the paraventricular nucleus of the hypothalamus of the rat (Cell Tissue Res., 221 (1981) 227-231). Behavioral analysis of one possible terminal field of the OXY-CCK coexistence, the caudal region of the mesencephalic ventral tegmentum, was undertaken to investigate the functional significance of this coexistence. Both OXY and CCK were found to induce grooming behaviors when microinjected into the ventral tegmental area (VTA) of awake rats. Combinations of one low and one higher dose of OXY and CCK yielded grooming scores which were not significantly different from grooming scores induced by each peptide alone. In this case of putative coexistence of two peptides without a 'classical' neurotransmitter, each peptide appears to have a behavioral function, and the interaction between the two peptides may be competitive. JF - Brain research AU - Kaltwasser, M T AU - Crawley, J N AD - Unit on Behavioral Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892. Y1 - 1987/11/17/ PY - 1987 DA - 1987 Nov 17 SP - 1 EP - 7 VL - 426 IS - 1 SN - 0006-8993, 0006-8993 KW - Oxytocin KW - 50-56-6 KW - Cholecystokinin KW - 9011-97-6 KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Animals KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Microinjections KW - Female KW - Oxytocin -- administration & dosage KW - Cholecystokinin -- administration & dosage KW - Tegmentum Mesencephali -- physiology KW - Grooming -- drug effects KW - Tegmentum Mesencephali -- drug effects KW - Oxytocin -- pharmacology KW - Cholecystokinin -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77852558?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research&rft.atitle=Oxytocin+and+cholecystokinin+induce+grooming+behavior+in+the+ventral+tegmentum+of+the+rat.&rft.au=Kaltwasser%2C+M+T%3BCrawley%2C+J+N&rft.aulast=Kaltwasser&rft.aufirst=M&rft.date=1987-11-17&rft.volume=426&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Brain+research&rft.issn=00068993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-01-25 N1 - Date created - 1988-01-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Immunohistochemical determination of inducibility phenotype with a monoclonal antibody to a methylcholanthrene-inducible isozyme of cytochrome P-450. AN - 81067232; 3664509 AB - A monoclonal antibody (MAb) to a methylcholanthrene (MC)-induced cytochrome P-450, designated MAb 1-7-1, was used for immunohistochemical staining of formalin-fixed tissues from oil- and MC-treated C57BL/6, DBA/2, and [(C57BL/6 X DBA/2) F1 X DBA/2] F2 mice. An avidin-biotin-peroxidase complex immunohistochemical technique was used. For controls, the tissues were also exposed to MAbs 1-48-5 and HyHel-9 (to egg white lysozyme). In liver, MAb 1-7-1 specifically stained the cytoplasm of centrilobular hepatocytes of C57BL/6 mice treated with MC (80 mg/kg) 48 h before kill; staining was not observed with vehicle-treated C57BL/6 mice, with oil- or MC-treated DBA/2 mice, or with comparable antibody concentrations of control MAbs 1-48-5 or HyHel-9. In the F2 mice, about 50% were expected to be MC inducible (AhbAhd). Inducibility phenotype was determined by measuring the conversion of [14C]MC to oxidized and conjugated products by liver homogenates. In freshly fixed material from MC-treated mice, those livers shown by the determination of phenotype to be inducible also stained with MAb 1-7-1, whereas those not induced were immunohistochemically negative. Furthermore, there was a significant positive correlation between degree of staining and the level of MC-metabolizing activity measured biochemically. The immunohistochemical procedure was also accurate in determination of inducibility phenotype of livers that had been in paraffin blocks for up to 2 yr if more concentrated antibody was used. In lung, MAb 1-7-1 stained specifically the alveolar walls and endothelium of blood vessels in MC-induced C57BL/6 mice only; the control MAbs and other mice gave negative results. Similarly, in kidney MAb 1-7-1 stained only glomeruli and interstitial tissue of MC-induced C57BL/6 mice and only endothelium of blood vessels in the colons of these mice. These observations are consistent with induction of the cytochrome P-450 recognized by MAb 1-7-1 in the endothelial cells of extrahepatic tissue. Immunohistochemical staining with MAb thus shows great promise for highly specific localization of particular species of cytochromes P-450 in tissues, for in situ quantification of these enzymes, and for determination of inducibility phenotype with fixed material. JF - Cancer research AU - Anderson, L M AU - Ward, J M AU - Park, S S AU - Jones, A B AU - Junker, J L AU - Gelboin, H V AU - Rice, J M AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick, Maryland 21701-1013. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 6079 EP - 6085 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Antibodies, Monoclonal KW - 0 KW - Isoenzymes KW - Methylcholanthrene KW - 56-49-5 KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Index Medicus KW - Animals KW - Mice KW - Mice, Inbred DBA KW - Phenotype KW - Biotransformation KW - Mice, Inbred C57BL KW - Crosses, Genetic KW - Enzyme Induction KW - Species Specificity KW - Immunohistochemistry KW - Female KW - Male KW - Intestinal Mucosa -- cytology KW - Liver -- cytology KW - Liver -- enzymology KW - Cytochrome P-450 Enzyme System -- genetics KW - Intestinal Mucosa -- enzymology KW - Lung -- cytology KW - Isoenzymes -- immunology KW - Kidney -- enzymology KW - Cytochrome P-450 Enzyme System -- biosynthesis KW - Methylcholanthrene -- metabolism KW - Isoenzymes -- genetics KW - Methylcholanthrene -- pharmacology KW - Isoenzymes -- biosynthesis KW - Cytochrome P-450 Enzyme System -- immunology KW - Kidney -- cytology KW - Lung -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81067232?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Immunohistochemical+determination+of+inducibility+phenotype+with+a+monoclonal+antibody+to+a+methylcholanthrene-inducible+isozyme+of+cytochrome+P-450.&rft.au=Anderson%2C+L+M%3BWard%2C+J+M%3BPark%2C+S+S%3BJones%2C+A+B%3BJunker%2C+J+L%3BGelboin%2C+H+V%3BRice%2C+J+M&rft.aulast=Anderson&rft.aufirst=L&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=6079&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Molecular dosimetry of DNA adduct formation and cell toxicity in rat nasal mucosa following exposure to the tobacco specific nitrosamine 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and their relationship to induction of neoplasia. AN - 81066913; 3664508 AB - The molecular dosimetry of O6-methylguanine (O6MG) formation in DNA and cytotoxicity in respiratory and olfactory mucosa was determined during administration of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) to male Fischer 344 rats. The dose response for O6MG formation differed considerably between respiratory and olfactory mucosa. The dose response was nonlinear in respiratory mucosa where the slope of the curve was very large for doses of NNK ranging from 0.3 to 3.0 mg/kg but much smaller in the dose range of 10 to 100 mg/kg. In contract, the dose response in the olfactory mucosa did not demonstrate such a large change in slope over the same dose range. The concentration of O6MG formed to dose of NNK ratio, an index of efficiency of alkylation, increased dramatically only in the respiratory mucosa as the dose of NNK was decreased from 100 to 0.3 mg/kg. The concentration of O6MG was four times greater in respiratory than olfactory mucosa after treatment of rats with 1 mg/kg NNK. Alkylation in the two regions of the nose became similar as the dose of NNK was increased. In rats treated for up to 12 days with NNK (10 mg/kg/day), the concentration of O6MG was 60 to 90% greater in respiratory than olfactory mucosa throughout treatment. Regional differences in the amount of O6MG formed may stem from the presence of a low Km pathway for biotransformation of NNK in the cells of the respiratory mucosa. This conclusion is supported by autoradiographic studies. Four h after treatment with 1 mg/kg [3H]NNK, silver grains were more heavily concentrated in respiratory than olfactory epithelium. Histopathological examination of the nasal passages revealed dose related, cell specific differences in toxicity following treatment of rats with 10, 30, or 100 mg/kg NNK for 12 days. No toxicity was observed in the nose when 1 mg/kg NNK was administered. Bowman's glands underlying the olfactory mucosa and Steno's glands were the most sensitive sites for toxicity, exhibiting necrosis after as little as 2 days of treatment with 10 mg/kg NNK. Damage to these glands progressed in a dose- and time-dependent manner. Respiratory epithelium exhibited only mild toxicity while basal cell metaplasia was evident in olfactory epithelium. Rats treated with NNK for 20 weeks (50 mg/kg, three times a week) had a 45% incidence of carcinomas in the olfactory region. These neoplasms appeared to arise from Bowman's glands. In contrast, there was only a 5% incidence of malignant neoplasia and a 29% incidence of benign neoplasia in the respiratory region.(ABSTRACT TRUNCATED AT 400 WORDS) JF - Cancer research AU - Belinsky, S A AU - Walker, V E AU - Maronpot, R R AU - Swenberg, J A AU - Anderson, M W AD - Laboratory of Biochemical Risk Analysis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 6058 EP - 6065 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Carcinogens KW - 0 KW - Nitrosamines KW - 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone KW - 7S395EDO61 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Necrosis KW - Methylation KW - Male KW - Plants, Toxic KW - Respiratory Tract Neoplasms -- chemically induced KW - Nitrosamines -- toxicity KW - Nasal Mucosa -- pathology KW - DNA -- metabolism KW - Nitrosamines -- metabolism KW - Tobacco KW - Nasal Mucosa -- drug effects KW - Respiratory Tract Neoplasms -- pathology KW - DNA -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81066913?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Molecular+dosimetry+of+DNA+adduct+formation+and+cell+toxicity+in+rat+nasal+mucosa+following+exposure+to+the+tobacco+specific+nitrosamine+4-%28N-methyl-N-nitrosamino%29-1-%283-pyridyl%29-1-butanone+and+their+relationship+to+induction+of+neoplasia.&rft.au=Belinsky%2C+S+A%3BWalker%2C+V+E%3BMaronpot%2C+R+R%3BSwenberg%2C+J+A%3BAnderson%2C+M+W&rft.aulast=Belinsky&rft.aufirst=S&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=6058&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Intrathecal administration of 4-hydroperoxycyclophosphamide in rhesus monkeys. AN - 81063064; 3664493 AB - The preactivated cyclophosphamide analogue, 4-HC, does not require activation by hepatic microsomal enzymes to express its cytotoxic activity and therefore, unlike cyclophosphamide, may be useful for the regional therapy of cancer. In the present study, the pharmacokinetics and toxicology of 4-HC were studied following intraventricular administration of 0.4 mg to rhesus monkeys with chronic indwelling Ommaya reservoirs. 4-HC was measured in cerebrospinal fluid (CSF) and plasma with a high-performance liquid chromatography assay utilizing a fluorometric detector following derivatization with m-aminophenol. The mean peak level of 4-HC in ventricular CSF was 100 microM 5 min after administration. The drug was cleared rapidly and the elimination was monoexponential with a mean half-life of 22 min. The mean clearance from CSF (0.33 ml/min) was 10-fold higher than CSF bulk flow. The drug was distributed throughout the subarachnoid space with lumbar levels approaching ventricular levels by 60 min. Neither acute nor chronic neurotoxicity or systemic toxicity was observed during the 6-wk observation period. Concentrations of 4-HC demonstrated to be cytocidal in vitro against human breast cancer, lymphoid leukemia, and rhabdomyosarcoma were readily achieved in CSF following intraventricular administration. This study demonstrates that intraventricular therapy with 4-HC is feasible and suggests that further study of this approach in the clinical setting should be considered. JF - Cancer research AU - Arndt, C A AU - Colvin, O M AU - Balis, F M AU - Lester, C M AU - Johnson, G AU - Poplack, D G AD - Pediatric Branch, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 5932 EP - 5934 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Antineoplastic Agents KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - perfosfamide KW - U880A4FUDA KW - Index Medicus KW - Drug Screening Assays, Antitumor KW - Animals KW - Cell Survival -- drug effects KW - Injections, Spinal KW - Macaca mulatta KW - Male KW - Cell Line KW - Cyclophosphamide -- administration & dosage KW - Cyclophosphamide -- analogs & derivatives KW - Antineoplastic Agents -- administration & dosage KW - Cyclophosphamide -- toxicity KW - Cyclophosphamide -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81063064?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Intrathecal+administration+of+4-hydroperoxycyclophosphamide+in+rhesus+monkeys.&rft.au=Arndt%2C+C+A%3BColvin%2C+O+M%3BBalis%2C+F+M%3BLester%2C+C+M%3BJohnson%2C+G%3BPoplack%2C+D+G&rft.aulast=Arndt&rft.aufirst=C&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=5932&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Inhibition by bryostatin 1 of the phorbol ester-induced blockage of differentiation in hexamethylene bisacetamide-treated Friend erythroleukemia cells. AN - 81055615; 3478136 AB - Phorbol esters inhibit chemically induced differentiation in Friend erythroleukemia cells. This study examines the effect of the macrocyclic lactone bryostatin 1 on phorbol ester responses in a Friend erythroleukemia cell clone, PS 7. In several biological systems, bryostatin 1 was reported to mimic phorbol ester action, including activation of protein kinase C, but in HL-60 cells it blocked phorbol ester-induced differentiation. We report here that bryostatin 1 blocks phorbol ester action in Friend cells (clone PS 7), a second differentiating system. In this system, in contrast to HL-60 cells, the phorbol esters inhibit rather than induce differentiation. Bryostatin 1 restores the differentiation response [50% effective dose, 15 +/- 3.5 nM (SEM)] as well as blocks a second phorbol ester effect, induction of cellular adherence. The inhibition of erythroid differentiation by dexamethasone, a nonphorbol compound whose action presumably is not protein kinase C mediated, is unaffected by bryostatin 1. Although bryostatin 1 inhibits [3H]phorbol 12,13-dibutyrate binding in intact Friend erythroleukemia cell clone PS 7, the mechanism for the antagonism of phorbol ester action by bryostatin 1 in Friend cells cannot be explained by simple competition at the binding site. JF - Cancer research AU - Dell'Aquila, M L AU - Nguyen, H T AU - Herald, C L AU - Pettit, G R AU - Blumberg, P M AD - Molecular Mechanisms of Tumor Promotion Section, National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 6006 EP - 6009 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Acetamides KW - 0 KW - Antineoplastic Agents KW - Bryostatins KW - Carcinogens KW - Lactones KW - Macrolides KW - Phorbol Esters KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - bryostatin 1 KW - 37O2X55Y9E KW - hexamethylene bisacetamide KW - LA133J59VU KW - Index Medicus KW - Animals KW - Kinetics KW - Humans KW - Leukemia, Erythroblastic, Acute -- pathology KW - Mice KW - Cell Differentiation -- drug effects KW - Cell Line KW - Phorbol Esters -- pharmacology KW - Carcinogens -- pharmacology KW - Acetamides -- pharmacology KW - Phorbol Esters -- antagonists & inhibitors KW - Antineoplastic Agents -- pharmacology KW - Leukemia, Experimental -- pathology KW - Lactones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81055615?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Inhibition+by+bryostatin+1+of+the+phorbol+ester-induced+blockage+of+differentiation+in+hexamethylene+bisacetamide-treated+Friend+erythroleukemia+cells.&rft.au=Dell%27Aquila%2C+M+L%3BNguyen%2C+H+T%3BHerald%2C+C+L%3BPettit%2C+G+R%3BBlumberg%2C+P+M&rft.aulast=Dell%27Aquila&rft.aufirst=M&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=6006&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Comparison of human and rat hepatocyte metabolism and mutagenic activation of 2-acetylaminofluorene. AN - 81055500; 2822235 AB - A method has been developed to assess the metabolism and mutagenic activation of carcinogens using human and rodent hepatocytes in vitro. A slicing technique which was especially useful for nonperfusable biopsy and resected surgical human liver tissue was used to prepare the hepatocytes. Metabolites of the model carcinogen 2-acetylaminofluorene (AAF) produced by human and rat hepatocytes were similar and consisted primarily of 2-aminofluorene with ring hydroxylated products at the 1-, 3-, 5/9-, 7-, and 8-positions produced in addition to N-hydroxy-AAF. Sulphate and glucuronide conjugates of ring-hydroxylated metabolites and 2-aminofluorene were detected. Metabolism and cell-mediated Salmonella mutagenicity illustrated interindividual variation with human hepatocytes. Levels of metabolism and mutagenesis were generally higher with human hepatocytes compared to rat hepatocyte results. The increased levels of metabolism and mutagenesis of AAF by human hepatocytes compared to rat hepatocytes probably indicates a different sensitivity to hepatocarcinogenic effects of AAF on humans as compared to rats. Understanding differences and similarities between human and rodent carcinogen activation capabilities should be useful in the extrapolation of rodent carcinogenesis data to humans. JF - Cancer research AU - Rudo, K AU - Meyers, W C AU - Dauterman, W AU - Langenbach, R AD - Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 5861 EP - 5867 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Mutagens KW - 0 KW - 2-Acetylaminofluorene KW - 9M98QLJ2DL KW - Index Medicus KW - Rats KW - Animals KW - Mutagenicity Tests KW - Biotransformation KW - Humans KW - In Vitro Techniques KW - Salmonella typhimurium -- drug effects KW - Biopsy KW - Species Specificity KW - Male KW - Female KW - Liver Neoplasms -- pathology KW - Liver Neoplasms -- metabolism KW - Liver -- pathology KW - Carcinoma, Hepatocellular -- metabolism KW - 2-Acetylaminofluorene -- pharmacology KW - 2-Acetylaminofluorene -- metabolism KW - Carcinoma, Hepatocellular -- pathology KW - Liver -- metabolism KW - Liver Neoplasms -- secondary KW - Mutation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81055500?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Comparison+of+human+and+rat+hepatocyte+metabolism+and+mutagenic+activation+of+2-acetylaminofluorene.&rft.au=Rudo%2C+K%3BMeyers%2C+W+C%3BDauterman%2C+W%3BLangenbach%2C+R&rft.aulast=Rudo&rft.aufirst=K&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=5861&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - The chemotherapy of lymphomas: looking back, moving forward--the Richard and Hinda Rosenthal Foundation award lecture. AN - 81055038; 3311358 JF - Cancer research AU - De Vita, V T AU - Hubbard, S M AU - Longo, D L AD - National Cancer Institute, Bethesda, Maryland 20892. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 5810 EP - 5824 VL - 47 IS - 22 SN - 0008-5472, 0008-5472 KW - Procarbazine KW - 35S93Y190K KW - Mechlorethamine KW - 50D9XSG0VR KW - Vincristine KW - 5J49Q6B70F KW - Prednisone KW - VB0R961HZT KW - Index Medicus KW - Animals KW - Procarbazine -- administration & dosage KW - Mechlorethamine -- administration & dosage KW - Humans KW - Vincristine -- administration & dosage KW - Prednisone -- administration & dosage KW - Hodgkin Disease -- drug therapy KW - Lymphoma -- drug therapy KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/81055038?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=The+chemotherapy+of+lymphomas%3A+looking+back%2C+moving+forward--the+Richard+and+Hinda+Rosenthal+Foundation+award+lecture.&rft.au=De+Vita%2C+V+T%3BHubbard%2C+S+M%3BLongo%2C+D+L&rft.aulast=De+Vita&rft.aufirst=V&rft.date=1987-11-15&rft.volume=47&rft.issue=22&rft.spage=5810&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1987-12-17 N1 - Date created - 1987-12-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Control of animal pain and distress in cancer and toxicologic research. AN - 77867968; 3692969 JF - Journal of the American Veterinary Medical Association AU - Montgomery, C A AD - Toxicologic Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709. Y1 - 1987/11/15/ PY - 1987 DA - 1987 Nov 15 SP - 1277 EP - 1281 VL - 191 IS - 10 SN - 0003-1488, 0003-1488 KW - Index Medicus KW - Rats KW - Animals KW - Guinea Pigs KW - Mice KW - Research Design KW - Toxicology KW - Neoplasms, Experimental KW - Cricetinae KW - Pain -- prevention & control KW - Animal Welfare KW - Pain -- veterinary KW - Stress, Physiological -- prevention & control KW - Stress, Physiological -- veterinary UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/77867968?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Veterinary+Medical+Association&rft.atitle=Control+of+animal+pain+and+distress+in+cancer+and+toxicologic+research.&rft.au=Montgomery%2C+C+A&rft.aulast=Montgomery&rft.aufirst=C&rft.date=1987-11-15&rft.volume=191&rft.issue=10&rft.spage=1277&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Veterinary+Medical+Association&rft.issn=00031488&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 1988-02-02 N1 - Date created - 1988-02-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-17 ER - TY - JOUR T1 - Effects of ouabain on NIH/3T3 cells transformed with retroviral oncogenes and on human tumor cell lines. AN - 77833691; 3679592 AB - Both murine and human cell lines transformed by the v-Ki-ras gene have been shown to be much more sensitive to the toxic effects of the cardiac glycoside ouabain than their respective controls. This differential toxicity has previously been used in the isolation of flat revertant clones from populations of Kirsten murine sarcoma virus transformed NIH/3T3 cells. Here, we have undertaken a further characterization of this phenomenon in murine and human tumor cells. Two different techniques, a 51Cr-release assay and a quantitative Crystal violet elution assay, have been employed to compare the sensitivities to ouabain of normal and v-Ki-ras-transformed NIH/3T3 cells. In each assay, ras-transformed NIH/3T3 cell lines displayed an increased sensitivity to ouabain as compared to the parental NIH/3T3 cell line, both in dose-response and in time-course experiments. In a separate study, ouabain was also able to inhibit the growth in semi-solid medium of 2 v-Ki-ras-transformed NIH/3T3 cell lines (DT and K-NIH) in a dose-dependent fashion. The same concentrations of ouabain were effective in both the 51Cr-release and Crystal violet assays. To address the question of whether increased sensitivity to ouabain is a specific resul