TY - CPAPER T1 - Fully Automatic Masking of Displacement Encoded (DENSE) Images T2 - 22nd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2005) AN - 40107065; 4000918 JF - 22nd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2005) AU - Wen, H Y1 - 2005/09/15/ PY - 2005 DA - 2005 Sep 15 KW - Automation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=22nd+Annual+Scientific+Meeting+of+the+European+Society+for+Magnetic+Resonance+in+Medicine+and+Biology+%28ESMRMB+2005%29&rft.atitle=Fully+Automatic+Masking+of+Displacement+Encoded+%28DENSE%29+Images&rft.au=Wen%2C+H&rft.aulast=Wen&rft.aufirst=H&rft.date=2005-09-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=22nd+Annual+Scientific+Meeting+of+the+European+Society+for+Magnetic+Resonance+in+Medicine+and+Biology+%28ESMRMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.esmrmb.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Thrombospondin-1 inhibits endothelial cell responses to nitric oxide in a cGMP-dependent manner. AN - 68585170; 16150726 AB - Redox signaling plays an important role in the positive regulation of angiogenesis by vascular endothelial growth factor, but its role in signal transduction by angiogenesis inhibitors is less clear. Using muscle explants in 3D culture, we found that explants from mice lacking the angiogenesis inhibitor thrombospondin-1 (TSP1) exhibit exaggerated angiogenic responses to an exogenous NO donor, which could be reversed by providing exogenous TSP1. To define the basis for inhibition by TSP1, we examined the effects of TSP1 on several proangiogenic responses of endothelial cells to NO. NO has a biphasic effect on endothelial cell proliferation. The positive effect at low doses of NO is sensitive to inhibition of cGMP signaling and picomolar concentrations of TSP1. NO stimulates both directed (chemotactic) and random (chemokinetic) motility of endothelial cells in a cGMP-dependent manner. TSP1 potently inhibits chemotaxis stimulated by NO. Low doses of NO also stimulate adhesion of endothelial cells on type I collagen in a cGMP-dependent manner. TSP1 potently inhibits this response both upstream and downstream of cGMP. NO-stimulated endothelial cell responses are inhibited by recombinant type 1 repeats of TSP1 and a CD36 agonist antibody but not by the N-terminal portion of TSP1, suggesting that CD36 or a related receptor mediates these effects. These results demonstrate a potent antagonism between TSP1 and proangiogenic signaling downstream of NO. Further elucidation of this inhibitory signaling pathway may identify new molecular targets to regulate pathological angiogenesis. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Isenberg, Jeff S AU - Ridnour, Lisa A AU - Perruccio, Elizabeth M AU - Espey, Michael G AU - Wink, David A AU - Roberts, David D AD - Laboratory of Pathology and Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 SP - 13141 EP - 13146 VL - 102 IS - 37 SN - 0027-8424, 0027-8424 KW - Nitric Oxide Donors KW - 0 KW - Thrombospondin 1 KW - Nitric Oxide KW - 31C4KY9ESH KW - Cyclic GMP KW - H2D2X058MU KW - Index Medicus KW - Cell Movement KW - Animals KW - Neovascularization, Physiologic KW - Umbilical Veins -- cytology KW - Mice KW - Cell Proliferation KW - Mice, Knockout KW - Nitric Oxide Donors -- pharmacology KW - Pectoralis Muscles -- cytology KW - Cells, Cultured KW - Mice, Inbred C57BL KW - Drug Antagonism KW - Cell Adhesion KW - Cyclic GMP -- analysis KW - Thrombospondin 1 -- physiology KW - Endothelium, Vascular -- drug effects KW - Endothelium, Vascular -- cytology KW - Cyclic GMP -- physiology KW - Nitric Oxide -- pharmacology KW - Thrombospondin 1 -- deficiency KW - Thrombospondin 1 -- pharmacology KW - Endothelium, Vascular -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68585170?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Thrombospondin-1+inhibits+endothelial+cell+responses+to+nitric+oxide+in+a+cGMP-dependent+manner.&rft.au=Isenberg%2C+Jeff+S%3BRidnour%2C+Lisa+A%3BPerruccio%2C+Elizabeth+M%3BEspey%2C+Michael+G%3BWink%2C+David+A%3BRoberts%2C+David+D&rft.aulast=Isenberg&rft.aufirst=Jeff&rft.date=2005-09-13&rft.volume=102&rft.issue=37&rft.spage=13141&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-09 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nat Med. 2000 Jan;6(1):41-8 [10613822] Matrix Biol. 2005 Apr;24(2):110-23 [15890262] Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2604-9 [11226286] J Pharmacol Exp Ther. 2001 Dec;299(3):818-24 [11714864] FASEB J. 2002 May;16(7):706-8 [11978735] Methods Enzymol. 2002;352:280-95 [12125354] Am J Physiol Lung Cell Mol Physiol. 2002 Oct;283(4):L671-7 [12225941] Mol Pharmacol. 2002 Oct;62(4):927-35 [12237340] Circulation. 2001 Apr 24;103(16):2102-7 [11319202] Antioxid Redox Signal. 2002 Oct;4(5):783-4 [12470505] Am J Physiol Heart Circ Physiol. 2003 Jan;284(1):H92-H100 [12388327] Matrix Biol. 2003 Mar;22(1):63-71 [12714043] Mol Cell Biol. 2003 Aug;23(16):5726-37 [12897144] Free Radic Biol Med. 2003 Aug 15;35(4):381-96 [12899940] Clin Cancer Res. 2003 Nov 1;9(14):5358-69 [14614021] Int J Biochem Cell Biol. 2004 Mar;36(3):460-71 [14687924] J Biol Chem. 2004 Jan 23;279(4):2550-8 [14600153] Circ Res. 2004 Mar 5;94(4):462-70 [14699013] Biochem Biophys Res Commun. 2004 May 28;318(2):520-8 [15120632] Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8894-9 [15178764] J Biol Chem. 2004 Aug 13;279(33):34311-22 [15184388] Circulation. 2004 Aug 31;110(9):1128-33 [15313948] J Cell Biol. 1989 Sep;109(3):1309-19 [2768342] J Cell Biol. 1990 Aug;111(2):765-72 [1696271] J Cell Biol. 1993 Jul;122(2):497-511 [7686555] J Cell Biochem. 1993 Sep;53(1):74-84 [8227183] J Cell Physiol. 1996 Jul;168(1):217-27 [8647918] J Cell Sci. 1996 Oct;109 ( Pt 10):2499-508 [8923211] J Cell Biol. 1997 Aug 11;138(3):707-17 [9245797] J Pept Res. 1997 Sep;50(3):210-21 [9309585] J Clin Invest. 1997 Dec 15;100(12):3131-9 [9399960] Circ Res. 1998 Feb 9;82(2):139-46 [9468184] Cancer. 1998 Feb 15;82(4):632-8 [9477093] J Clin Invest. 1998 Mar 1;101(5):982-92 [9486968] Proc Natl Acad Sci U S A. 1998 May 26;95(11):6343-8 [9600967] J Clin Invest. 1998 Jun 1;101(11):2567-78 [9616228] Cancer Res. 1998 Jul 15;58(14):3154-62 [9679984] Cancer Res. 1998 Aug 15;58(16):3751-6 [9721889] J Biol Chem. 1998 Sep 4;273(36):23504-8 [9722588] J Lab Clin Med. 1998 Dec;132(6):519-29 [9851743] Nature. 1999 Jun 10;399(6736):597-601 [10376602] Nature. 1999 Jun 10;399(6736):601-5 [10376603] Atherosclerosis. 1999 May;144(1):49-57 [10381277] Curr Pharm Des. 2005;11(7):849-66 [15777239] Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13147-52 [16141331] J Biol Chem. 2000 Aug 18;275(33):25723-32 [10851246] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Deletion of XPC leads to lung tumors in mice and is associated with early events in human lung carcinogenesis. AN - 68585081; 16141330 AB - Chromosome 3p and 1p deletions are among the most frequent genetic changes in human lung cancer and although candidate tumor suppressor genes have been identified in these regions, no causative correlations have been drawn between deletion or mutation of these and lung carcinogenesis. We identify XPC and Gadd45a as genes within each of these regions involved in lung tumor initiation and progression, respectively. One hundred percent of XPC-/- mice develop multiple spontaneous lung tumors with a minority progressing to non-small cell lung adenocarcinoma, occasionally with metastasis to adjacent lymph nodes. Deletion of Gadd45a alone does not lead to increased lung tumors in mice, but coupled with an XPC deletion, it results in lung tumor progression. Analysis of published data indicated allelic loss of XPC in most human lung tumors and allelic loss of Gadd45a in some human lung and other cancer types. Because DNA repair capacity is compromised in XPC+/- cells, it is possible that the loss of a single XPC allele in the human lung might confer a mutator phenotype. Coupled with cigarette carcinogens, decreased DNA repair would lead to additional mutations in genes such as p53 that are frequent targets in lung cancer. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Hollander, M Christine AU - Philburn, Robyn T AU - Patterson, Andrew D AU - Velasco-Miguel, Susana AU - Friedberg, Errol C AU - Linnoila, R Ilona AU - Fornace, Albert J AD - National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. ch96b@nih.gov Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 SP - 13200 EP - 13205 VL - 102 IS - 37 SN - 0027-8424, 0027-8424 KW - Cell Cycle Proteins KW - 0 KW - DNA-Binding Proteins KW - GADD45A protein, human KW - Gadd45a protein, mouse KW - Nuclear Proteins KW - Xpc protein, mouse KW - XPC protein, human KW - 156533-34-5 KW - Index Medicus KW - Chromosomes, Human, Pair 1 KW - Animals KW - Chromosomes, Human, Pair 3 KW - Alleles KW - DNA Damage KW - Humans KW - Mice KW - Chromosome Mapping KW - Male KW - Female KW - Mice, Knockout KW - Carcinoma, Non-Small-Cell Lung -- pathology KW - Cell Cycle Proteins -- physiology KW - Lung Neoplasms -- etiology KW - Nuclear Proteins -- genetics KW - Cell Cycle Proteins -- genetics KW - DNA-Binding Proteins -- genetics KW - Lung Neoplasms -- genetics KW - DNA-Binding Proteins -- physiology KW - Nuclear Proteins -- physiology KW - Lung Neoplasms -- pathology KW - Gene Deletion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68585081?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Deletion+of+XPC+leads+to+lung+tumors+in+mice+and+is+associated+with+early+events+in+human+lung+carcinogenesis.&rft.au=Hollander%2C+M+Christine%3BPhilburn%2C+Robyn+T%3BPatterson%2C+Andrew+D%3BVelasco-Miguel%2C+Susana%3BFriedberg%2C+Errol+C%3BLinnoila%2C+R+Ilona%3BFornace%2C+Albert+J&rft.aulast=Hollander&rft.aufirst=M&rft.date=2005-09-13&rft.volume=102&rft.issue=37&rft.spage=13200&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-09 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mutat Res. 2000 Mar 20;459(2):99-108 [10725660] Nucleic Acids Res. 2002 Aug 15;30(16):3624-31 [12177305] Int J Cancer. 2000 Jun 1;86(5):690-4 [10797292] Cancer Res. 2000 Sep 1;60(17):4894-906 [10987304] Biol Chem. 2002 Jun;383(6):907-14 [12222680] Oncogene. 2002 Oct 7;21(45):6915-35 [12362274] Cancer Biol Ther. 2002 May-Jun;1(3):232-6 [12432269] Cancer Res. 2002 Dec 15;62(24):7305-15 [12499274] Lung Cancer. 2003 Mar;39(3):273-7 [12609565] Cancer Res. 2003 Mar 1;63(5):902-5 [12615700] Mol Biotechnol. 2003 Jun;24(2):141-56 [12746555] Cancer Cell. 2003 Sep;4(3):181-9 [14522252] Mutat Res. 2003 Nov;544(2-3):107-14 [14644313] Annu Rev Physiol. 2004;66:647-63 [14977417] Cancer Res. 2004 Apr 1;64(7):2307-16 [15059877] Cancer Res. 2004 May 1;64(9):3096-102 [15126346] Am J Physiol Lung Cell Mol Physiol. 2004 Oct;287(4):L685-703 [15355860] Cancer Res. 2004 Sep 15;64(18):6432-7 [15374951] Lab Anim. 1971 Oct;5(2):179-92 [5166568] JAMA. 1995 Feb 15;273(7):558-63 [7837389] Curr Biol. 1996 Dec 1;6(12):1691-4 [8994835] Mutat Res. 1997 Mar 4;374(1):1-9 [9067411] Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9463-8 [9256505] Carcinogenesis. 1998 Mar;19(3):501-7 [9525286] Exp Lung Res. 1998 Jul-Aug;24(4):481-97 [9659579] Oncogene. 1998 Jun 11;16(23):3083-6 [9662341] Cancer Res. 1999 Feb 15;59(4):771-5 [10029060] Br J Cancer. 1999 Mar;79(9-10):1468-74 [10188892] Prog Exp Tumor Res. 1999;35:37-52 [10377750] Oncogene. 1999 Sep 20;18(38):5318-24 [10498884] Nat Genet. 1999 Oct;23(2):176-84 [10508513] Cancer Epidemiol Biomarkers Prev. 2004 Nov;13(11 Pt 1):1788-93 [15533908] Cancer Res. 2005 Jan 1;65(1):92-8 [15665283] Mol Carcinog. 2005 Feb;42(2):65-92 [15682379] Genes Dev. 2005 Mar 15;19(6):643-64 [15769940] Int J Cancer. 2005 Jun 20;115(3):478-83 [15700316] Int J Cancer. 2005 Jul 10;115(5):807-13 [15729698] Oncogene. 2000 Oct 12;19(43):5034-7 [11042691] Int J Cancer. 2000 Dec 15;88(6):932-7 [11093817] Cancer Res. 2001 Mar 15;61(6):2487-91 [11289119] Cancer Res. 2001 Apr 15;61(8):3321-5 [11309287] Photodermatol Photoimmunol Photomed. 2001 Apr;17(2):47-54 [11338401] Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10250-5 [11517343] Toxicol Pathol. 2001 Nov-Dec;29(6):653-61 [11794381] Oncogene. 2002 May 2;21(19):3020-8 [12082532] Mol Cell Biol. 2000 May;20(10):3705-14 [10779360] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Cancer Incidence Among Pesticide Applicators Exposed to Metolachlor in the Agricultural Health Study T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39793705; 4025841 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Rusieck, J A AU - Hou, L AU - Lee, W J AU - Blair, A AU - Dosemeci, M AU - Lubin, J AU - Bonner, M AU - Samanic, C AU - Hoppin, J A AU - Sandler, D P AU - Alavanja, M.C.R. Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - Pesticides KW - Agriculture KW - Metolachlor KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39793705?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Cancer+Incidence+Among+Pesticide+Applicators+Exposed+to+Metolachlor+in+the+Agricultural+Health+Study&rft.au=Rusieck%2C+J+A%3BHou%2C+L%3BLee%2C+W+J%3BBlair%2C+A%3BDosemeci%2C+M%3BLubin%2C+J%3BBonner%2C+M%3BSamanic%2C+C%3BHoppin%2C+J+A%3BSandler%2C+D+P%3BAlavanja%2C+M.C.R.&rft.aulast=Rusieck&rft.aufirst=J&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Review of Human Data on Effects of DDT, and Some New Results T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39791633; 4025673 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Longnecker, M P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - DDT KW - Reviews KW - Insecticides KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39791633?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Review+of+Human+Data+on+Effects+of+DDT%2C+and+Some+New+Results&rft.au=Longnecker%2C+M+P&rft.aulast=Longnecker&rft.aufirst=M&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Epidemiologic Evaluation of Measurement Data in the Presence of Detection Limits T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39728252; 4025690 DE: JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Lubin, J H AU - Colt, J S AU - Camann, D AU - Davis, S AU - Cerhan, J R AU - Severson, R K AU - Bernstein, L AU - Hartge, P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39728252?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Epidemiologic+Evaluation+of+Measurement+Data+in+the+Presence+of+Detection+Limits&rft.au=Lubin%2C+J+H%3BColt%2C+J+S%3BCamann%2C+D%3BDavis%2C+S%3BCerhan%2C+J+R%3BSeverson%2C+R+K%3BBernstein%2C+L%3BHartge%2C+P&rft.aulast=Lubin&rft.aufirst=J&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - An Overview of DDT World Policy T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39648183; 4025672 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Longnecker, M P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - DDT KW - Reviews KW - Insecticides KW - Policies KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39648183?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=An+Overview+of+DDT+World+Policy&rft.au=Longnecker%2C+M+P&rft.aulast=Longnecker&rft.aufirst=M&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Strengthening Institutional Capacity for Effective Research Stewardship T2 - 9th Global Forum for Health Research AN - 39762092; 4045914 DE: JF - 9th Global Forum for Health Research AU - Krotoski, Danuta Y1 - 2005/09/12/ PY - 2005 DA - 2005 Sep 12 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39762092?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=9th+Global+Forum+for+Health+Research&rft.atitle=Strengthening+Institutional+Capacity+for+Effective+Research+Stewardship&rft.au=Krotoski%2C+Danuta&rft.aulast=Krotoski&rft.aufirst=Danuta&rft.date=2005-09-12&rft.volume=122&rft.issue=1-3&rft.spage=741&rft.isbn=&rft.btitle=&rft.title=Applied+Biochemistry+and+Biotechnology&rft.issn=02732289&rft_id=info:doi/10.1385%2FABAB%3A122%3A1-3%3A0741 L2 - http://www.globalforumhealth.org/filesupld/forum9/Edited%20programme%20Fina l.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Role of Stem Cells in Hepatocarcinogenesis T2 - 42nd Congress of the European Societies of Toxicology (EUROTOX 2005) AN - 40055777; 3975930 JF - 42nd Congress of the European Societies of Toxicology (EUROTOX 2005) AU - Thorgeirsson, S S Y1 - 2005/09/11/ PY - 2005 DA - 2005 Sep 11 KW - Stem cells KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40055777?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=42nd+Congress+of+the+European+Societies+of+Toxicology+%28EUROTOX+2005%29&rft.atitle=The+Role+of+Stem+Cells+in+Hepatocarcinogenesis&rft.au=Thorgeirsson%2C+S+S&rft.aulast=Thorgeirsson&rft.aufirst=S&rft.date=2005-09-11&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=42nd+Congress+of+the+European+Societies+of+Toxicology+%28EUROTOX+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eurotox2005.org/eurotox/index.jsp?place=Menu01&news_cat_id=-1&la yout=0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - The common A467T mutation in the human mitochondrial DNA polymerase (POLG) compromises catalytic efficiency and interaction with the accessory subunit. AN - 68571983; 16024923 AB - Among the nearly 50 disease mutations in the gene for the catalytic subunit of human DNA polymerase gamma, POLG, the A467T substitution is the most common and has been found in 0.6% of the Belgian population. The A467T mutation is associated with a wide range of mitochondrial disorders, including Alpers syndrome, juvenile spinocerebellar ataxia-epilepsy syndrome, and progressive external ophthalmoplegia, each with vastly different clinical presentations, tissue specificities, and ages of onset. The A467T mutant enzyme possesses only 4% of wild-type DNA polymerase activity, and the catalytic defect is manifest primarily through a 6-fold reduction in kcat with minimal effect on exonuclease function. Human DNA polymerase gamma (pol gamma) requires association of a 55-kDa accessory subunit for enhanced DNA binding and highly processive DNA synthesis. However, the A467T mutant enzyme failed to interact with and was not stimulated by the accessory subunit, as judged by processivity, heat inactivation, and N-ethylmaleimide protection assays in vitro. Thermolysin digestion and immunoprecipitation experiments further indicate weak association of the subunits for A467T pol gamma. This is the first example of a mutation in POLG that disrupts physical association of the pol gamma subunits. We propose that reduced polymerase activity and loss of accessory subunit interaction are responsible for the depletion and deletion of mitochondrial DNA observed in patients with this POLG mutation. JF - The Journal of biological chemistry AU - Chan, Sherine S L AU - Longley, Matthew J AU - Copeland, William C AD - Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31341 EP - 31346 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - DNA, Mitochondrial KW - 0 KW - Nucleic Acid Synthesis Inhibitors KW - Threonine KW - 2ZD004190S KW - POLG protein, human KW - EC 2.7.7.- KW - DNA-Directed DNA Polymerase KW - EC 2.7.7.7 KW - Thermolysin KW - EC 3.4.24.27 KW - Ethylmaleimide KW - O3C74ACM9V KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Animals KW - Threonine -- genetics KW - Spodoptera KW - Humans KW - Thermolysin -- metabolism KW - Protein Structure, Quaternary KW - Mutagenesis, Site-Directed KW - Hot Temperature KW - Amino Acid Substitution -- genetics KW - Point Mutation KW - Alanine -- genetics KW - Ethylmaleimide -- pharmacology KW - Cell Line KW - DNA, Mitochondrial -- metabolism KW - Catalytic Domain -- genetics KW - DNA-Directed DNA Polymerase -- genetics KW - DNA-Directed DNA Polymerase -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571983?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahnpnewyorktimes&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Television+This+Week%3A+OF+SPECIAL+INTEREST+Today+Monday+Tuesday+Wednesday+Thursday+Saturday+Channel+Information+TODAY--SUNDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+6+Morning+Afternoon+Evening+MONDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+7+Morning+Afternoon+Evening+TUESDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+8+Morning+Afternoon+Evening+WEDNESDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+9+Morning+Afternoon+Evening+THURSDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+10+Morning+Afternoon+Evening+FRIDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+11+Morning+Afternoon+Evening+SATURDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+12+Morning+Afternoon+Evening&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1977-11-06&rft.volume=&rft.issue=&rft.spage=D37&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - 15S-Lipoxygenase-2 mediates arachidonic acid-stimulated adhesion of human breast carcinoma cells through the activation of TAK1, MKK6, and p38 MAPK. AN - 68571132; 16000313 AB - The dietary cis-polyunsaturated fatty acid, arachidonic acid, stimulates adhesion of metastatic human breast carcinoma cells (MDA-MB-435) to the extracellular matrix, but the molecular mechanisms by which fatty acids modify the behavior of these cells are unclear. Exposure to arachidonic acid activates multiple signaling pathways. Activation of p38 mitogen-activated protein kinase (p38 MAPK) is required for increased cell adhesion to type IV collagen, and this activation is sensitive to inhibitors of lipoxygenases, suggesting a requirement for arachidonic acid metabolism. The goals of the current study were to identify the one or more key metabolites of arachidonic acid that are responsible for activation of p38 MAPK and to elucidate the upstream kinases that lead to p38 MAPK activation. High performance liquid chromatographic analysis revealed that MDA-MB-435 cells metabolize exogenous arachidonic acid predominantly to 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). Immunoblot analysis with antibodies specific to 15(S)-lipoxygenase-1 (LOX-1) and 15(S)-lipoxygenase-2 (LOX-2) demonstrated the expression of 15-LOX-2, but not 15-LOX-1, in these tumor cells. A LOX inhibitor, nordihydroguaiaretic acid, attenuated production of 15(S)-HETE and inhibited the phosphorylation of p38 MAPK following exposure to arachidonic acid. In contrast, overexpression of LOX-2 sensitized the cells to the addition of arachidonic acid, leading to increased activation of p38 MAPK. Addition of exogenous 15(S)-HETE to MDA-MB-435 cells stimulated cell adhesion to type IV collagen and activated the p38 MAPK pathway, including the upstream kinases transforming growth factor-beta1-activated protein kinase-1 (TAK1) and MAPK kinase 6. Transfection of these cells with a dominant negative form of TAK1 blocked arachidonic acid-stimulated p38 MAPK phosphorylation. These data demonstrate that 15(S)-LOX-2 generation of 15(S)-HETE activates specific growth factor receptor-related signaling pathways, thereby initiating signal transduction events leading to increased cell adhesion to the extracellular matrix. JF - The Journal of biological chemistry AU - Nony, Paul A AU - Kennett, Sarah B AU - Glasgow, Wayne C AU - Olden, Kenneth AU - Roberts, John D AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31413 EP - 31419 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - Hydroxyeicosatetraenoic Acids KW - 0 KW - Lipoxygenase Inhibitors KW - Arachidonic Acid KW - 27YG812J1I KW - Masoprocol KW - 7BO8G1BYQU KW - Arachidonate 15-Lipoxygenase KW - EC 1.13.11.33 KW - p38 Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - MAP Kinase Kinase Kinase 5 KW - EC 2.7.11.25 KW - MAP Kinase Kinase Kinases KW - MAP kinase kinase kinase 7 KW - MAP3K5 protein, human KW - MAP Kinase Kinase 6 KW - EC 2.7.12.2 KW - MAP2K6 protein, human KW - Index Medicus KW - Phosphorylation KW - Cell Adhesion -- physiology KW - Humans KW - MAP Kinase Kinase Kinase 5 -- metabolism KW - Enzyme Activation -- physiology KW - Carcinoma -- enzymology KW - Cell Line, Tumor KW - Hydroxyeicosatetraenoic Acids -- metabolism KW - Female KW - Chromatography, High Pressure Liquid KW - Masoprocol -- pharmacology KW - MAP Kinase Kinase Kinases -- metabolism KW - Arachidonic Acid -- physiology KW - Breast Neoplasms -- enzymology KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Arachidonic Acid -- metabolism KW - MAP Kinase Kinase 6 -- metabolism KW - Arachidonate 15-Lipoxygenase -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=15S-Lipoxygenase-2+mediates+arachidonic+acid-stimulated+adhesion+of+human+breast+carcinoma+cells+through+the+activation+of+TAK1%2C+MKK6%2C+and+p38+MAPK.&rft.au=Nony%2C+Paul+A%3BKennett%2C+Sarah+B%3BGlasgow%2C+Wayne+C%3BOlden%2C+Kenneth%3BRoberts%2C+John+D&rft.aulast=Nony&rft.aufirst=Paul&rft.date=2005-09-09&rft.volume=280&rft.issue=36&rft.spage=31413&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. AN - 68571020; 16002405 AB - DNA polymerase lambda (pol lambda) is a member of the X family of DNA polymerases that has been implicated in both base excision repair and non-homologous end joining through in vitro studies. However, to date, no phenotype has been associated with cells deficient in this DNA polymerase. Here we show that pol lambda null mouse fibroblasts are hypersensitive to oxidative DNA damaging agents, suggesting a role of pol lambda in protection of cells against the cytotoxic effects of oxidized DNA. Additionally, pol lambda co-immunoprecipitates with an oxidized base DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1), and localizes to oxidative DNA lesions in situ. From these data, we conclude that pol lambda protects cells against oxidative stress and suggest that it participates in oxidative DNA damage base excision repair. JF - The Journal of biological chemistry AU - Braithwaite, Elena K AU - Kedar, Padmini S AU - Lan, Li AU - Polosina, Yaroslava Y AU - Asagoshi, Kenjiro AU - Poltoratsky, Vladimir P AU - Horton, Julie K AU - Miller, Holly AU - Teebor, George W AU - Yasui, Akira AU - Wilson, Samuel H AD - Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31641 EP - 31647 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - Oxidants KW - 0 KW - 5-hydroxymethyluracil KW - 4433-40-3 KW - Pentoxyl KW - 7LCS1FW4JV KW - DNA Polymerase beta KW - EC 2.7.7.- KW - DNA polymerase beta2 KW - DNA Glycosylases KW - EC 3.2.2.- KW - SMUG1 protein, human KW - Uracil-DNA Glycosidase KW - Index Medicus KW - Oxidation-Reduction KW - Animals KW - Pentoxyl -- pharmacology KW - Pentoxyl -- analogs & derivatives KW - HeLa Cells KW - Humans KW - DNA Glycosylases -- metabolism KW - Oxidants -- chemistry KW - Mice KW - Cell Line KW - DNA Repair -- genetics KW - DNA Repair -- physiology KW - DNA Polymerase beta -- deficiency KW - DNA Damage -- physiology KW - DNA Polymerase beta -- genetics KW - DNA Polymerase beta -- physiology KW - DNA Damage -- genetics KW - Fibroblasts -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571020?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=DNA+polymerase+lambda+protects+mouse+fibroblasts+against+oxidative+DNA+damage+and+is+recruited+to+sites+of+DNA+damage%2Frepair.&rft.au=Braithwaite%2C+Elena+K%3BKedar%2C+Padmini+S%3BLan%2C+Li%3BPolosina%2C+Yaroslava+Y%3BAsagoshi%2C+Kenjiro%3BPoltoratsky%2C+Vladimir+P%3BHorton%2C+Julie+K%3BMiller%2C+Holly%3BTeebor%2C+George+W%3BYasui%2C+Akira%3BWilson%2C+Samuel+H&rft.aulast=Braithwaite&rft.aufirst=Elena&rft.date=2005-09-09&rft.volume=280&rft.issue=36&rft.spage=31641&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Neonatal Disconnection of the Hippocampus Alters Prefrontal Cortical Function: Implications for Schizophrenia T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39663745; 3974591 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Lipska, Barbara Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Mental disorders KW - Schizophrenia KW - Neonates KW - Hippocampus KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39663745?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Neonatal+Disconnection+of+the+Hippocampus+Alters+Prefrontal+Cortical+Function%3A+Implications+for+Schizophrenia&rft.au=Lipska%2C+Barbara&rft.aulast=Lipska&rft.aufirst=Barbara&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Neuropeptide Y in Anxiety and Alcoholism T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39663668; 3974582 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Heilig, Markus Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Alcoholism KW - Drug abuse KW - Neuropeptide Y KW - Anxiety KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39663668?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Neuropeptide+Y+in+Anxiety+and+Alcoholism&rft.au=Heilig%2C+Markus&rft.aulast=Heilig&rft.aufirst=Markus&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Galanin; a Novel Therapeutic Target for Stress-Related Disorders? T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39658626; 3974583 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Holmes, Andrew Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Galanin KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39658626?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Galanin%3B+a+Novel+Therapeutic+Target+for+Stress-Related+Disorders%3F&rft.au=Holmes%2C+Andrew&rft.aulast=Holmes&rft.aufirst=Andrew&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Synthesis and Structure-Activity Relationship of a Novel Series of Aminoalkylindoles with Potential for Imaging the Neuronal Cannabinoid Receptor by Positron Emission Tomography AN - 19390738; 7158832 AB - A new series of CB sub(1) ligands with high binding affinity (K sub(i) = 0.7-100 nM) and moderate lipophilicity (cLogD sub(7.4)) in the range of 2.1-4.5 has been synthesized. A structure-activity relationship study demonstrated that for the studied set of aminoalkylindoles, the molecular dipole of the ground state conformation within the series was inversely related to the affinity. The racemic ligand with highest affinity (0.7 nM), 3-(4-fluoronaphthoyl) -1-(N-methylpiperidin-2-ylmethyl) indole, was radiolabeled with super(18)F. This radioligand specifically labeled CB sub(1) receptors in mouse brain and accumulated in regions of high versus low CB sub(1) receptor density in a ratio of 1.6. The displaceable radioactivity of one enantiomer in the brains of mice determined in a pretreatment study using the CB sub(1) antagonist N-(piperidinyl)-5-(4-chlorophenyl) -1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (SR141716) was nearly double that of the racemate for the same determination; therefore, the active enantiomer is a candidate for PET studies in animals. A pretreatement study for the other enantiomer found no displaceable radioactivity in the same group of mice; this result suggested the enantiomer was inactive. JF - Journal of Medicinal Chemistry AU - Willis, P G AU - Pavlova, O A AU - Chefer, SI AU - Vaupel, D B AU - Mukhin, A G AU - Horti, A G AD - Neuroimaging Research Branch, Intramural Research Program, National Institute on Drug Abuse, NIH, DHHS, 5500 Nathan Shock Driver, Baltimore, Mayland 21224, USA Y1 - 2005/09/08/ PY - 2005 DA - 2005 Sep 08 SP - 5813 EP - 5822 VL - 48 IS - 18 SN - 0022-2623, 0022-2623 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Brain KW - Enantiomers KW - Indole KW - Receptor density KW - Positron emission tomography KW - Radioactivity KW - Cannabinoid CB1 receptors KW - Structure-activity relationships KW - Conformation KW - W 30910:Imaging KW - N3 11008:Neurochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19390738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Synthesis+and+Structure-Activity+Relationship+of+a+Novel+Series+of+Aminoalkylindoles+with+Potential+for+Imaging+the+Neuronal+Cannabinoid+Receptor+by+Positron+Emission+Tomography&rft.au=Willis%2C+P+G%3BPavlova%2C+O+A%3BChefer%2C+SI%3BVaupel%2C+D+B%3BMukhin%2C+A+G%3BHorti%2C+A+G&rft.aulast=Willis&rft.aufirst=P&rft.date=2005-09-08&rft.volume=48&rft.issue=18&rft.spage=5813&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm0502743 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Enantiomers; Cannabinoid CB1 receptors; Brain; Radioactivity; Structure-activity relationships; Positron emission tomography; Neuroimaging; Conformation; Receptor density; Indole DO - http://dx.doi.org/10.1021/jm0502743 ER - TY - JOUR T1 - Reduced Cocaine Self-Administration in Muscarinic M sub(5) Acetylcholine Receptor-Deficient Mice AN - 17404556; 6529455 AB - The reinforcing effects of cocaine have been related to increased extracellular concentrations of dopamine in the ventral striatum. Several studies suggest that M sub(5) muscarinic receptors facilitate striatal dopamine release. We tested the hypothesis that the reinforcing effects of cocaine are decreased in M sub(5) receptor-deficient mice using chronic intravenous cocaine self-administration in extensively backcrossed mice. We also assessed whether operant performance generally, rather than cocaine self-administration specifically, was altered in the mutant mice. To this end, we evaluated both food-maintained operant behavior and cocaine self-administration under a fixed ratio 1 and a progressive ratio (PR) schedule of reinforcement. We also evaluated acquisition of self-administration in experimentally naive mice using several doses of cocaine. M sub(5) receptor deletion decreased self-administration of low to moderate doses of cocaine under a PR schedule of reinforcement and diminished acquisition of self-administration of a low dose in experimentally naive mice. We found no differences between genotypes in food-maintained behavior. The present study extends our previous findings using backcrossed mice and covering various experimental conditions. Our results indicate that M sub(5) receptor deletion diminished the reinforcing effects of low doses of cocaine and identified specific conditions under which this may be observed. JF - Journal of Neuroscience AU - Thomsen, Morgane AU - Woldbye, David PD AU - Woertwein, Gitta AU - Fink-Jensen, Anders AU - Wess, Juergen AU - Caine, SBarak AD - Alcohol and Drug Abuse Research Center, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, Laboratory of Neuropsychiatry, Rigshospitalet University Hospital and Department of Pharmacology, University of Copenhagen, DK-2100 Copenhagen, Denmark, and Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/09/07/ PY - 2005 DA - 2005 Sep 07 SP - 8141 EP - 8149 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 36 SN - 0270-6474, 0270-6474 KW - CSA Neurosciences Abstracts; Toxicology Abstracts KW - Dopamine KW - Neostriatum KW - Acetylcholine receptors (muscarinic) KW - Reinforcement KW - Genotypes KW - Cocaine KW - Drug addiction KW - Drug self-administration KW - X 24180:Social poisons & drug abuse KW - N3 11139:Toxicological and psychoactive drug correlates UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17404556?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Reduced+Cocaine+Self-Administration+in+Muscarinic+M+sub%285%29+Acetylcholine+Receptor-Deficient+Mice&rft.au=Thomsen%2C+Morgane%3BWoldbye%2C+David+PD%3BWoertwein%2C+Gitta%3BFink-Jensen%2C+Anders%3BWess%2C+Juergen%3BCaine%2C+SBarak&rft.aulast=Thomsen&rft.aufirst=Morgane&rft.date=2005-09-07&rft.volume=25&rft.issue=36&rft.spage=8141&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Dopamine; Acetylcholine receptors (muscarinic); Neostriatum; Reinforcement; Genotypes; Drug addiction; Cocaine; Drug self-administration ER - TY - JOUR T1 - Inhibiting farnesylation of progerin prevents the characteristic nuclear blebbing of Hutchinson-Gilford progeria syndrome. AN - 68565457; 16129833 AB - Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is characterized by dramatic premature aging and accelerated cardiovascular disease. HGPS is almost always caused by a de novo point mutation in the lamin A gene (LMNA) that activates a cryptic splice donor site, producing a truncated mutant protein termed "progerin." WT prelamin A is anchored to the nuclear envelope by a farnesyl isoprenoid lipid. Cleavage of the terminal 15 aa and the farnesyl group releases mature lamin A from this tether. In contrast, this cleavage site is deleted in progerin. We hypothesized that retention of the farnesyl group causes progerin to become permanently anchored in the nuclear membrane, disrupting proper nuclear scaffolding and causing the characteristic nuclear blebbing seen in HGPS cells. Also, we hypothesized that blocking farnesylation would decrease progerin toxicity. To test this hypothesis, the terminal CSIM sequence in progerin was mutated to SSIM, a sequence that cannot be farnesylated. SSIM progerin relocalized from the nuclear periphery into nucleoplasmic aggregates and produced no nuclear blebbing. Also, blocking farnesylation of authentic progerin in transiently transfected HeLa, HEK 293, and NIH 3T3 cells with farnesyltransferase inhibitors (FTIs) restored normal nuclear architecture. Last, treatment of both early- and late-passage human HGPS fibroblasts with FTIs resulted in significant reductions in nuclear blebbing. Our results suggest that treatment with FTIs represents a potential therapy for patients with HGPS. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Capell, Brian C AU - Erdos, Michael R AU - Madigan, James P AU - Fiordalisi, James J AU - Varga, Renee AU - Conneely, Karen N AU - Gordon, Leslie B AU - Der, Channing J AU - Cox, Adrienne D AU - Collins, Francis S AD - Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, 50 South Drive, MSC 8004, Bethesda, MD 20892-8004, USA. Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 12879 EP - 12884 VL - 102 IS - 36 SN - 0027-8424, 0027-8424 KW - Enzyme Inhibitors KW - 0 KW - Lamin Type A KW - Alkyl and Aryl Transferases KW - EC 2.5.- KW - Farnesyltranstransferase KW - EC 2.5.1.29 KW - Index Medicus KW - Protein Prenylation -- drug effects KW - Animals KW - Humans KW - Mutation -- genetics KW - Enzyme Inhibitors -- pharmacology KW - Alkyl and Aryl Transferases -- antagonists & inhibitors KW - Mice KW - Cell Line KW - Alkyl and Aryl Transferases -- metabolism KW - Lamin Type A -- chemistry KW - Cell Nucleus -- pathology KW - Progeria -- pathology KW - Progeria -- metabolism KW - Cell Nucleus -- metabolism KW - Lamin Type A -- genetics KW - Lamin Type A -- metabolism KW - Progeria -- drug therapy KW - Cell Nucleus -- drug effects KW - Progeria -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68565457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Inhibiting+farnesylation+of+progerin+prevents+the+characteristic+nuclear+blebbing+of+Hutchinson-Gilford+progeria+syndrome.&rft.au=Capell%2C+Brian+C%3BErdos%2C+Michael+R%3BMadigan%2C+James+P%3BFiordalisi%2C+James+J%3BVarga%2C+Renee%3BConneely%2C+Karen+N%3BGordon%2C+Leslie+B%3BDer%2C+Channing+J%3BCox%2C+Adrienne+D%3BCollins%2C+Francis+S&rft.aulast=Capell&rft.aufirst=Brian&rft.date=2005-09-06&rft.volume=102&rft.issue=36&rft.spage=12879&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-09-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Pathol. 2004 Nov;204(4):478-88 [15495262] Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8963-8 [15184648] Arch Pathol Lab Med. 1981 Jul;105(7):384-6 [6894691] Development. 1989 Feb;105(2):365-78 [2680424] J Cell Biol. 1990 May;110(5):1489-99 [2335559] Cell. 1990 Jul 13;62(1):81-8 [2194674] J Cell Biol. 1993 Nov;123(3):501-12 [8227121] J Cell Sci. 1994 Apr;107 ( Pt 4):1019-29 [8056827] J Biol Chem. 1995 Dec 22;270(51):30611-8 [8530497] Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4454-8 [8633088] J Biol Chem. 1997 Apr 11;272(15):10232-9 [9092572] J Biol Chem. 1997 May 30;272(22):14093-7 [9162034] J Biol Chem. 1997 May 30;272(22):14459-64 [9162087] J Biol Chem. 1998 Jun 12;273(24):15030-4 [9614111] Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18111-6 [15608054] Nat Rev Mol Cell Biol. 2005 Jan;6(1):21-31 [15688064] Nat Med. 2005 Apr;11(4):440-5 [15750600] Curr Opin Drug Discov Devel. 2004 Jul;7(4):478-86 [15338957] Hum Mol Genet. 2004 Oct 15;13(20):2493-503 [15317753] J Cell Biol. 1999 Nov 29;147(5):913-20 [10579712] Postgrad Med J. 2001 May;77(907):312-7 [11320273] Curr Med Chem. 2001 Oct;8(12):1419-36 [11562275] Genes Dev. 2002 Mar 1;16(5):533-47 [11877373] Nat Genet. 2002 May;31(1):94-9 [11923874] Curr Opin Pharmacol. 2002 Aug;2(4):388-93 [12127871] Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13049-54 [12235369] Nature. 2003 May 15;423(6937):293-8 [12714972] Curr Top Med Chem. 2003;3(10):1103-14 [12769711] Curr Opin Genet Dev. 2003 Jun;13(3):223-30 [12787783] Science. 2003 Jun 27;300(5628):2055 [12702809] Hum Mol Genet. 2003 Aug 15;12(16):1995-2001 [12913070] Nat Rev Cancer. 2003 Dec;3(12):945-51 [14737124] J Pediatr. 1972 Apr;80(4):697-724 [4552697] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Clusters of mutations from transient hypermutability. AN - 68564476; 16118275 AB - Collections of mutants usually contain more mutants bearing multiple mutations than expected from the mutant frequency and a random distribution of mutations. This excess is seen in a variety of organisms and also after DNA synthesis in vitro. The excess is unlikely to originate in mutator mutants but rather from transient hypermutability resulting from a perturbation of one of the many transactions that maintain genetic fidelity. The multiple mutations are sometimes clustered and sometimes randomly distributed. We model some spectra as populations comprising a majority with a low mutation frequency and a minority with a high mutation frequency. In the case of mutants produced in vitro by a bacteriophage RB69 mutator DNA polymerase, mutants with two mutations are in approximately 10-fold excess and mutants with three mutations are in even greater excess. However, phenotypically undetectable mutations seen only as hitchhikers with detectable mutations are approximately 5-fold more frequent than mutants bearing detectable mutations, indicating that they arose in a subpopulation with a higher mutation frequency. Excess multiple mutations may contribute critically to carcinogenesis and to adaptive mutation, including the adaptations of pathogens as they move from host to host. In the case of the rapidly mutating riboviruses, the viral population appears to be composed of a majority with a mutation frequency substantially lower than the average and a minority with a huge mutational load. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Drake, John W AU - Bebenek, Anna AU - Kissling, Grace E AU - Peddada, Shyamal AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. drake@niehs.nih.gov Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 12849 EP - 12854 VL - 102 IS - 36 SN - 0027-8424, 0027-8424 KW - RNA KW - 63231-63-0 KW - DNA-Directed DNA Polymerase KW - EC 2.7.7.7 KW - Index Medicus KW - Bacteriophages -- enzymology KW - Animals KW - Humans KW - DNA Mutational Analysis KW - Genome KW - Neoplasms -- genetics KW - RNA -- genetics KW - DNA-Directed DNA Polymerase -- metabolism KW - Mutation -- genetics KW - Mutagenesis -- genetics KW - Evolution, Molecular UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68564476?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Clusters+of+mutations+from+transient+hypermutability.&rft.au=Drake%2C+John+W%3BBebenek%2C+Anna%3BKissling%2C+Grace+E%3BPeddada%2C+Shyamal&rft.aulast=Drake&rft.aufirst=John&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=40&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-09-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Gen Genet. 1984;198(2):177-8 [6394963] Genetics. 2005 Apr;169(4):1815-24 [15695359] Proc Natl Acad Sci U S A. 1987 May;84(10):3354-8 [3554239] Proc Natl Acad Sci U S A. 1988 Nov;85(21):8126-30 [3054881] J Biol Chem. 1989 Oct 5;264(28):16948-56 [2476448] J Mol Biol. 1989 Sep 20;209(2):195-204 [2685319] J Bacteriol. 1990 Jun;172(6):3009-14 [2160935] J Virol. 1990 Aug;64(8):3960-2 [1695258] Genetics. 1991 Nov;129(3):957-62 [1752431] J Mol Biol. 1991 Dec 20;222(4):925-36 [1762158] Mol Gen Genet. 1992 Nov;235(2-3):173-8 [1465091] Genetics. 1993 Aug;134(4):1031-8 [8375646] Nucleic Acids Res. 1993 Nov 11;21(22):5212-20 [7504813] Genetics. 1994 Oct;138(2):263-70 [7828810] Methods Enzymol. 1995;262:217-32 [8594349] Science. 1996 Nov 15;274(5290):1208-11 [8895473] J Bacteriol. 1997 Jan;179(2):417-22 [8990293] J Biol Chem. 1997 Mar 14;272(11):7345-51 [9054433] Nature. 1997 Jun 12;387(6634):700-2 [9192893] EMBO J. 1997 Jun 2;16(11):3303-11 [9214645] Science. 1997 Sep 5;277(5331):1523-6 [9278518] Environ Mol Mutagen. 1997;30(3):273-86 [9366905] Biochemistry. 1998 Feb 24;37(8):2111-9 [9485358] J Mol Biol. 1998 Apr 24;278(1):135-46 [9571039] Genetics. 1998 Apr;148(4):1483-90 [9560368] Genetics. 1998 Apr;148(4):1619-26 [9560381] Genetics. 1998 Apr;148(4):1667-86 [9560386] Mutat Res. 1998 May 25;400(1-2):89-97 [9685594] Mutagenesis. 1998 Sep;13(5):487-97 [9800194] Science. 1999 Jan 29;283(5402):641 [9988656] Environ Mol Mutagen. 1999;33(2):132-43 [10217067] Genetics. 1999 Jun;152(2):485-93 [10353893] Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13910-3 [10570172] Genetics. 2000 Mar;154(3):959-70 [10757746] Science. 2000 Apr 21;288(5465):514-7 [10775110] Mutat Res. 2000 Sep 18;452(2):219-29 [11024481] Mutat Res. 2000 Dec 20;457(1-2):93-104 [11106801] J Biol Chem. 2001 Mar 30;276(13):10387-97 [11133987] Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7928-33 [11427720] Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1437-42 [11818556] J Virol. 2002 Jun;76(11):5822-8 [11992012] J Biol Chem. 2002 Sep 20;277(38):35550-60 [12121998] Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14878-83 [12403824] Genetics. 2002 Nov;162(3):1003-18 [12454051] Mutat Res. 2002 Dec 29;510(1-2):153-68 [12459451] Genetics. 2002 Dec;162(4):1505-11 [12524327] J Virol. 2003 Mar;77(5):2946-55 [12584319] Cold Spring Harb Symp Quant Biol. 2000;65:21-9 [12760017] Mutat Res. 2004 Oct 4;554(1-2):223-40 [15450421] Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6862-7 [10359804] J Virol. 1999 Jul;73(7):5326-32 [10364279] Acta Pathol Microbiol Scand. 1960;48:113-20 [14408281] J Gen Microbiol. 1959 Dec;21:530-49 [14440412] J Biol Chem. 1985 May 10;260(9):5787-96 [3988773] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Roles of Drosophila DJ-1 in survival of dopaminergic neurons and oxidative stress. AN - 68552430; 16139214 AB - The loss of dopaminergic neurons in the substantia nigra is the pathological hallmark of Parkinson's disease (PD). While the etiology of sporadic PD remains elusive, an inherited form of early-onset familial PD is linked to mutations of DJ-1. To understand the biological function of DJ-1 and its relevance to the pathogenesis of PD, we investigated the function of DJ-1 using Drosophila. Drosophila possesses two homologs of human DJ-1: DJ-1alpha and DJ-1beta. We found that DJ-1alpha is expressed predominantly in the testis, while DJ-1beta is ubiquitously present in most tissues, resembling the expression pattern of human DJ-1. Loss-of-function DJ-1beta mutants demonstrated an extended survival of dopaminergic neurons and resistance to paraquat stress, but showed acute sensitivity to hydrogen peroxide treatment. We showed a compensatory upregulation of DJ-1alpha expression in the brain of the DJ-1beta mutant and demonstrated that overexpression of DJ-1alpha in dopaminergic neurons is sufficient to confer protection against paraquat insult. These results suggest that Drosophila homologs of DJ-1 play critical roles in the survival of dopaminergic neurons and response to oxidative stress. JF - Current biology : CB AU - Menzies, Fiona M AU - Yenisetti, Sarat C AU - Min, Kyung-Tai AD - Neurogenetics Branch MSC 3705, Building 35, Room 2A 1002 NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 1578 EP - 1582 VL - 15 IS - 17 SN - 0960-9822, 0960-9822 KW - DJ-1alpha protein, Drosophila KW - 0 KW - DJ-1beta protein, Drosophila KW - Drosophila Proteins KW - Nerve Tissue Proteins KW - Hydrogen Peroxide KW - BBX060AN9V KW - Paraquat KW - PLG39H7695 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Hydrogen Peroxide -- toxicity KW - Gene Expression Profiling KW - Animals KW - Blotting, Western KW - Sequence Alignment KW - Molecular Sequence Data KW - Mutation -- genetics KW - Dopamine -- metabolism KW - Brain -- metabolism KW - Amino Acid Sequence KW - Immunohistochemistry KW - Paraquat -- toxicity KW - Neurons -- metabolism KW - Neurons -- drug effects KW - Oxidative Stress -- drug effects KW - Drosophila -- metabolism KW - Nerve Tissue Proteins -- metabolism KW - Oxidative Stress -- genetics KW - Gene Expression Regulation -- drug effects KW - Drosophila Proteins -- genetics KW - Nerve Tissue Proteins -- genetics KW - Drosophila -- genetics KW - Drosophila Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68552430?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+biology+%3A+CB&rft.atitle=Roles+of+Drosophila+DJ-1+in+survival+of+dopaminergic+neurons+and+oxidative+stress.&rft.au=Menzies%2C+Fiona+M%3BYenisetti%2C+Sarat+C%3BMin%2C+Kyung-Tai&rft.aulast=Menzies&rft.aufirst=Fiona&rft.date=2005-09-06&rft.volume=15&rft.issue=17&rft.spage=1578&rft.isbn=&rft.btitle=&rft.title=Current+biology+%3A+CB&rft.issn=09609822&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-23 N1 - Date created - 2005-09-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Pancreatic Cancer and Neural System Tumors (NSTS) in Melanoma-Prone Families with CDKN2A Mutations T2 - 6th World Congress on Melanoma AN - 40116180; 3994356 JF - 6th World Congress on Melanoma AU - Goldstein, A Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Mutation KW - Pancreatic cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40116180?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Pancreatic+Cancer+and+Neural+System+Tumors+%28NSTS%29+in+Melanoma-Prone+Families+with+CDKN2A+Mutations&rft.au=Goldstein%2C+A&rft.aulast=Goldstein&rft.aufirst=A&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Surgical Treatment in Horizontal Growth Phase Melanoma. Results of a Prospective Study at National Cancer Institute, Milan T2 - 6th World Congress on Melanoma AN - 40115246; 3994088 JF - 6th World Congress on Melanoma AU - Moglia, D AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Maurichi, A AU - Patuzzo, R AU - Pennacchioli, E AU - Santoro, N AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40115246?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Surgical+Treatment+in+Horizontal+Growth+Phase+Melanoma.+Results+of+a+Prospective+Study+at+National+Cancer+Institute%2C+Milan&rft.au=Moglia%2C+D%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BLiborio%2C+F%3BMaurichi%2C+A%3BPatuzzo%2C+R%3BPennacchioli%2C+E%3BSantoro%2C+N%3BSantinami%2C+M&rft.aulast=Moglia&rft.aufirst=D&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Radiofrequency Thermal Ablation of Liver Melanoma Metastases. A Study of 19 Cases T2 - 6th World Congress on Melanoma AN - 40082041; 3994193 JF - 6th World Congress on Melanoma AU - Maurichi, A AU - Bono, A AU - Bartoli, C AU - Liborio, F AU - Moglia, D AU - Patuzzo, R AU - Pennacchioli, E AU - Rebuffoni, G AU - Santinami, M AU - Garbagnati, F Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Ablation KW - Metastases KW - Melanoma KW - Liver KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40082041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Legal+Notice+2+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2000-11-29&rft.volume=&rft.issue=&rft.spage=G7&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Interplay of MC1R, ASIP, and DNA Repair in Sporadic and Familial Melanoma Risk in a Mediterranean population T2 - 6th World Congress on Melanoma AN - 40081760; 3994142 JF - 6th World Congress on Melanoma AU - Landi, M T AU - Kanetsky, P A AU - Tsang, S AU - Gold, B AU - Rebbeck, T AU - Hedayati, M AU - Grossman, L AU - Goldstein, A M AU - Calista, D AU - Pfeiffer, R M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Mediterranean KW - Melanoma KW - Mediterranean region KW - DNA repair KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40081760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Interplay+of+MC1R%2C+ASIP%2C+and+DNA+Repair+in+Sporadic+and+Familial+Melanoma+Risk+in+a+Mediterranean+population&rft.au=Landi%2C+M+T%3BKanetsky%2C+P+A%3BTsang%2C+S%3BGold%2C+B%3BRebbeck%2C+T%3BHedayati%2C+M%3BGrossman%2C+L%3BGoldstein%2C+A+M%3BCalista%2C+D%3BPfeiffer%2C+R+M&rft.aulast=Landi&rft.aufirst=M&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Predicting Absolute Risk of Melanoma: A Management Model for Use by Primary Care Providers T2 - 6th World Congress on Melanoma AN - 40078679; 3994451 JF - 6th World Congress on Melanoma AU - Fears, T R AU - Guerry AU - Pfeiffer, R M AU - Sagebiel, R W AU - Elder, D E AU - Halpern, A AU - Holly, E A AU - Hartge, P Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Models KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40078679?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+308+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2002-11-10&rft.volume=&rft.issue=&rft.spage=K12&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Radical Dissectione of Groin Basin After Positive SNB: Overtreatment or Standard Surgery? T2 - 6th World Congress on Melanoma AN - 40068300; 3994468 JF - 6th World Congress on Melanoma AU - Pennacchioli, E AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Ruggeri, R AU - Tragni, G AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Surgery KW - Basins KW - Groynes KW - Radicals KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40068300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Radical+Dissectione+of+Groin+Basin+After+Positive+SNB%3A+Overtreatment+or+Standard+Surgery%3F&rft.au=Pennacchioli%2C+E%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BMaurichi%2C+A%3BMoglia%2C+D%3BPatuzzo%2C+R%3BRuggeri%2C+R%3BTragni%2C+G%3BSantinami%2C+M&rft.aulast=Pennacchioli&rft.aufirst=E&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - KIR 2DS4 Genotypes in Sporadic and Familial Melanoma from Italy T2 - 6th World Congress on Melanoma AN - 40041699; 3994442 JF - 6th World Congress on Melanoma AU - Landi, M T AU - Hulley, B AU - Martin, P AU - Pfeiffer, R AU - Goldstein, A AU - Landi, G AU - Carrington, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Italy KW - Melanoma KW - Genotypes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041699?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+67+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1997-05-11&rft.volume=&rft.issue=&rft.spage=H47&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Etiologic and Other Factors Predicting Nevus-Associated Cutaneous Malignant Melanoma T2 - 6th World Congress on Melanoma AN - 40041638; 3994431 JF - 6th World Congress on Melanoma AU - Purdue, M AU - From, L AU - Armstrong, B AU - Kricker, A AU - Gallagher, R AU - McLaughlin, J AU - Klar, N AU - Marrett, L Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041638?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Etiologic+and+Other+Factors+Predicting+Nevus-Associated+Cutaneous+Malignant+Melanoma&rft.au=Poggi%2C+Sarah+H%3BPark%2C+Jane%3BToso%2C+Laura%3BAbebe%2C+Daniel%3BRoberson%2C+Robin%3BWoodard%2C+Jade+E%3BSpong%2C+Catherine+Y&rft.aulast=Poggi&rft.aufirst=Sarah&rft.date=2005-03-01&rft.volume=192&rft.issue=3&rft.spage=727&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=00029378&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Morphologic Evaluation of the Sentinel Node Does Not Correlate with Survival of Melanoma Patients T2 - 6th World Congress on Melanoma AN - 40039922; 3994275 JF - 6th World Congress on Melanoma AU - Santinami, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Roberto, M AU - Tragni, G AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Nodes KW - Survival KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40039922?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+81+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1988-09-30&rft.volume=&rft.issue=&rft.spage=D16&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Cutaneous Desmoplastic Melanoma T2 - 6th World Congress on Melanoma AN - 40037399; 3994087 JF - 6th World Congress on Melanoma AU - Maurichi, A AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Moglia, D AU - Patuzzo, R AU - Pennacchioli, E AU - Tragni, G AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40037399?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Cutaneous+Desmoplastic+Melanoma&rft.au=Maurichi%2C+A%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BLiborio%2C+F%3BMoglia%2C+D%3BPatuzzo%2C+R%3BPennacchioli%2C+E%3BTragni%2C+G%3BSantinami%2C+M&rft.aulast=Maurichi&rft.aufirst=A&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Regional Perfusion for Limbs' in Transit Metastases from Melanoma: Results in a Single Institution T2 - 6th World Congress on Melanoma AN - 40034500; 3994198 JF - 6th World Congress on Melanoma AU - Patuzzo, R AU - Bartoli, C AU - Bono, A AU - Deraco, M AU - Liborio, F AU - Maurichi, A AU - Moglia, D AU - Roberto, M AU - Santinami, M AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - Limbs KW - Perfusion KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40034500?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=DEATHS&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2008-11-12&rft.volume=&rft.issue=&rft.spage=B19&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Melanoma in Colombia T2 - 6th World Congress on Melanoma AN - 40033972; 3994124 JF - 6th World Congress on Melanoma AU - Acosta, A Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Colombia KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40033972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahnpnewyorktimes&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281857-1922%29&rft.atitle=Display+Ad+15+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1902-11-25&rft.volume=&rft.issue=&rft.spage=13&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281857-1922%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Sentinel Node Biopsy: Standard Treatment for Melanoma? Results at the National Cancer Institute of Milan T2 - 6th World Congress on Melanoma AN - 40033767; 3994091 JF - 6th World Congress on Melanoma AU - Santinami, M AU - Bartoli, C AU - Bono, A AU - Costanzo, P AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Santoro, N AU - Tragni, G AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Cancer KW - Biopsy KW - Nodes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40033767?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Sentinel+Node+Biopsy%3A+Standard+Treatment+for+Melanoma%3F+Results+at+the+National+Cancer+Institute+of+Milan&rft.au=Santinami%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BCostanzo%2C+P%3BMaurichi%2C+A%3BMoglia%2C+D%3BPatuzzo%2C+R%3BSantoro%2C+N%3BTragni%2C+G%3BPennacchioli%2C+E&rft.aulast=Santinami&rft.aufirst=M&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Adoptive Cell Transfer Therapy Following Lymphodepleting Chemotherapy for the Treatment of Patients with Refractory Metastatic Melanoma T2 - 6th World Congress on Melanoma AN - 40032078; 3994338 JF - 6th World Congress on Melanoma AU - Powell Jr, D AU - Rosenberg, S Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - Chemotherapy KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40032078?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Adoptive+Cell+Transfer+Therapy+Following+Lymphodepleting+Chemotherapy+for+the+Treatment+of+Patients+with+Refractory+Metastatic+Melanoma&rft.au=Powell+Jr%2C+D%3BRosenberg%2C+S&rft.aulast=Powell+Jr&rft.aufirst=D&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Temozolomide for the Treatment of Brain and Systemic Disease in Patients with Metastatic Melanoma. National Cancer Institute Slovakia-The First T2 - 6th World Congress on Melanoma AN - 40012962; 3994202 JF - 6th World Congress on Melanoma AU - Salek, T Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - temozolomide KW - Brain KW - Cancer KW - Disease control KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40012962?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Temozolomide+for+the+Treatment+of+Brain+and+Systemic+Disease+in+Patients+with+Metastatic+Melanoma.+National+Cancer+Institute+Slovakia-The+First&rft.au=Salek%2C+T&rft.aulast=Salek&rft.aufirst=T&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - 'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system AN - 17393636; 6513060 AB - DNA damage recognition represents a long-standing problem in the field of protein-DNA interactions. This article reviews our current knowledge of how damage recognition is achieved in bacterial nucleotide excision repair through the concerted action of the UvrA, UvrB, and UvrC proteins. JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis AU - Van Houten, B AU - Croteau, D L AU - DellaVecchia, MJ AU - Wang, H AU - Kisker, C AD - National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD D3-01, Research Triangle Park, NC 27709, USA, vanhout1@niehs.nih.gov Y1 - 2005/09/04/ PY - 2005 DA - 2005 Sep 04 SP - 92 EP - 117 VL - 577 IS - 1-2 SN - 0027-5107, 0027-5107 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - DNA damage KW - Molecular modelling KW - Nucleotide excision repair KW - Reviews KW - Nuclease KW - Mutagenesis KW - J 02310:Genetics & Taxonomy KW - N 14100:Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17393636?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.atitle=%27Close-fitting+sleeves%27%3A+DNA+damage+recognition+by+the+UvrABC+nuclease+system&rft.au=Van+Houten%2C+B%3BCroteau%2C+D+L%3BDellaVecchia%2C+MJ%3BWang%2C+H%3BKisker%2C+C&rft.aulast=Van+Houten&rft.aufirst=B&rft.date=2005-09-04&rft.volume=577&rft.issue=1-2&rft.spage=92&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.issn=00275107&rft_id=info:doi/10.1016%2Fj.mrfmmm.2005.03.013 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Molecular modelling; DNA damage; Nucleotide excision repair; Reviews; Nuclease; Mutagenesis DO - http://dx.doi.org/10.1016/j.mrfmmm.2005.03.013 ER - TY - JOUR T1 - Health risks and benefits of bis(4-chlorophenyl)-1,1,1-trichloroethane (DDT) AN - 17660363; 6499904 AB - DDT (bis[4-chlorophenyl]-1,1,1-trichloroethane) is a persistent insecticide that was used worldwide from the mid-1940s until its ban in the USA and other countries in the 1970s. When a global ban on DDT was proposed in 2001, several countries in sub-Saharan Africa claimed that DDT was still needed as a cheap and effective means for vector control. Although DDT is generally not toxic to human beings and was banned mainly for ecological reasons, subsequent research has shown that exposure to DDT at amounts that would be needed in malaria control might cause preterm birth and early weaning, abrogating the benefit of reducing infant mortality from malaria. Historically, DDT has had mixed success in Africa; only the countries that are able to find and devote substantial resources towards malaria control have made major advances. DDT might be useful in controlling malaria, but the evidence of its adverse effects on human health needs appropriate research on whether it achieves a favourable balance of risk versus benefit. JF - Lancet AU - Rogan, W J AU - Chen, A AD - Epidemiology Branch, US National Institute of Environmental Health Sciences, P O Box 12233, Research Triangle Park, NC 27709, USA, rogan@niehs.nih.gov Y1 - 2005/09/02/ PY - 2005 DA - 2005 Sep 02 SP - 763 EP - 773 VL - 366 IS - 9487 SN - 0099-5355, 0099-5355 KW - Risk Abstracts; Toxicology Abstracts; Health & Safety Science Abstracts KW - R2 23060:Medical and environmental health KW - H 12000:Epidemiology and Public Health KW - X 24136:Environmental impact UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17660363?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Thr105Ile%2C+a+functional+polymorphism+of+histamine+N-methyltransferase%2C+is+associated+with+alcoholism+in+two+independent+populations.&rft.au=Oroszi%2C+Gabor%3BEnoch%2C+Mary-Anne%3BChun%2C+Jeffrey%3BVirkkunen%2C+Matti%3BGoldman%2C+David&rft.aulast=Oroszi&rft.aufirst=Gabor&rft.date=2005-03-01&rft.volume=29&rft.issue=3&rft.spage=303&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Transcriptional Profiling Identifies Altered Intracellular Labile Iron Homeostasis as a Contributing Factor to the Toxicity of Adaphostin: Decreased Vascular Endothelial Growth Factor Secretion Is Independent of Hypoxia-Inducible Factor-1 Regulation AN - 745637228; 6529992 AB - PURPOSE: Adaphostin was developed as an inhibitor of the p210 super(bcr-abl) tyrosine kinase, but as its activity is not limited to tumor cell lines containing this translocation, transcriptional profiling was used as a tool to elucidate additional mechanisms responsible for adaphostin cytotoxicity. Experimental design: Profiles of drug-induced transcriptional changes were measured in three hematopoietic cell lines following 1 and 10 kmol/L adaphostin for 2 to 6 hours and then confirmed with real-time reverse transcription-PCR (2-24 hours). These data indicated altered iron homeostasis, and this was confirmed experimentally. Alteration of vascular endothelial growth factor (VEGF) secretion through hypoxia-inducible factor-1 (HIF-1) regulation was also investigated. RESULTS: Drug-induced genes included heat shock proteins and ubiquitins, but an intriguing response was the induction of ferritins. Measurement of the labile iron pool showed release of chelatable iron immediately after treatment with adaphostin and was quenched with the addition of an iron chelator. Pretreatment of cells with desferrioxamine and N-acetyl-cysteine reduced but did not ablate the sensitivity of the cells to adaphostin, and desferrioxamine was able to modulate adaphostin-induced activation of p38 and inactivation of AKT. VEGF secretion was shown to be reduced in cell lines after the addition of adaphostin but was not dependent on HIF-1. CONCLUSIONS: Adaphostin-induced cytotoxicity is caused in part by a rapid release of free iron, leading to redox perturbations and cell death. Despite this, reduced VEGF secretion was found to be independent of regulation by the redox responsive transcription factor HIF-1. Thus, adaphostin remains an interesting agent with the ability to kill tumor cells directly and modulate angiogenesis. JF - Clinical Cancer Research AU - Hose, Curtis AU - Kaur, Gurmeet AU - Sausville, Edward A AU - Monks, Anne AD - Authors' Affiliations: SAIC Frederick, Inc., Screening Technologies Branch, Laboratory of Functional Genomics, National Cancer Institute Frederick, Frederick, Maryland and Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, Maryland Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 6370 EP - 6381 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 17 SN - 1078-0432, 1078-0432 KW - Toxicology Abstracts KW - Vascular endothelial growth factor KW - Heat shock proteins KW - Data processing KW - Secretion KW - Angiogenesis KW - Transcription KW - Toxicity KW - Homeostasis KW - Chelating agents KW - Hypoxia-inducible factor 1 KW - Cytotoxicity KW - Tumor cell lines KW - Cell death KW - Transcription factors KW - Protein-tyrosine kinase KW - AKT protein KW - Hemopoiesis KW - Ferritin KW - Iron KW - Translocation KW - Deferoxamine KW - Ubiquitin KW - X 24360:Metals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/745637228?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Transcriptional+Profiling+Identifies+Altered+Intracellular+Labile+Iron+Homeostasis+as+a+Contributing+Factor+to+the+Toxicity+of+Adaphostin%3A+Decreased+Vascular+Endothelial+Growth+Factor+Secretion+Is+Independent+of+Hypoxia-Inducible+Factor-1+Regulation&rft.au=Hose%2C+Curtis%3BKaur%2C+Gurmeet%3BSausville%2C+Edward+A%3BMonks%2C+Anne&rft.aulast=Hose&rft.aufirst=Curtis&rft.date=2005-09-01&rft.volume=11&rft.issue=17&rft.spage=6370&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-07-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Vascular endothelial growth factor; Heat shock proteins; Data processing; Secretion; Angiogenesis; Transcription; Homeostasis; Toxicity; Chelating agents; Hypoxia-inducible factor 1; Cell death; Tumor cell lines; Cytotoxicity; Transcription factors; Protein-tyrosine kinase; AKT protein; Hemopoiesis; Ferritin; Translocation; Iron; Deferoxamine; Ubiquitin ER - TY - JOUR T1 - How to write an oncology manuscript. AN - 70175859; 16892095 AB - Publications may represent accomplishment in academic medicine, primary documentation of research data, evidence of expertise through writing an authoritative review paper or book chapter or a major determinant in achieving academic promotion and career development. Editors and reviewers appreciate receiving manuscripts that are easy to read and edit. Much of the information in journals instructions to authors is designed to accomplish that goal in ways that meet each journal's particular editorial needs. The CONsolidated Standards of Reporting Trial (CONSORT) statement is an important research tool that takes an evidence-based approach to improve the quality of reports of randomized trials. The guidance that follows provides a general background and rationale for preparing oncology manuscripts for any journal. Many of these guidelines are based on feedback provided by actual peer reviewers. Even before you start writing, it is good practice to review the typical sections of a manuscript The text of observational and experimental articles is usually (but not necessarily) divided into sections with the headings Introduction, Methods, Results, and Discussion. This so-called "IMRAD". The abstract of the manuscript is usually divided into background, purpose, patients and methods, results and conclusion. The section on patients and methods of an oncology manuscript should include the eligibility criteria for the patients, study design, treatment plan, baseline and treatment assessments and statistical analysis. The results include data on patient characteristics, tumor response, time to event measures, toxicity and dose administration. The conclusion must address the primary objective of the study. Authors will be able to address up front many issues regarding content, organization, presentation, and formatting, thereby increasing the likelihood of successful publication of their papers in peer-reviewed journals. JF - Journal of the Egyptian National Cancer Institute AU - Gaafar, Rabab AD - The Department of Medical Oncology, National Cancer Institute, Cairo University. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 132 EP - 138 VL - 17 IS - 3 SN - 1110-0362, 1110-0362 KW - Index Medicus KW - Humans KW - Writing -- standards KW - Publishing -- standards KW - Periodicals as Topic -- standards KW - Medical Oncology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70175859?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=Recovery+from+DSM-IV+alcohol+dependence%3A+United+States%2C+2001-2002.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BStinson%2C+Frederick+S%3BChou%2C+Patricia+S%3BHuang%2C+Boji%3BRuan%2C+W+June&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-03-01&rft.volume=100&rft.issue=3&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-14 N1 - Date created - 2006-08-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - [Mortality among workers in a cigarette factory in Lucca (Tuscany)]. TT - Studio di mortalità degli addetti alla manifattura del tabacco di Lucca. AN - 70160239; 16669164 AB - Aim of the present cohort study was to evaluate the mortality pattern among workers in a cigar and cigarette factory in Lucca, Italy. The study followed 2341 workers (1585 women and 756 men) registered in the company payrolls and employed for at least six months from 1 January 1960 through 1 January 1994. Follow-up was between the start of employment in the factory and 1 June 2002 (totally 74363,5 person-years). For both sexes, all-causes mortality was lower than expected (men: SMR= 0.8; CI95% 0.7-0.9; 158 deaths; women: SMR= 0.9; CI95% 0.8-1.0; 584 deaths) and no excess of mortality was reported for all malignant neoplasms. Among female workers, the frequency of deaths from pleural cancer was elevated at a statistically significant level (SMR= 6.0; CI95% 2.4-12.6; 5 deaths). One death for pleural cancer also occurred among men versus 0.4 expected. All women deceased from pleural cancer had been working in tobacco manufacturing for at least 30 years. The excess of pleural neoplasms reported in this study suggests the opportunity to evaluate the risk due to asbestos use in many manifacturing industries, especially where steam was used for extractive or warming purpose. JF - Epidemiologia e prevenzione AU - Parducci, Dino Ausilio AU - Puccetti, Monica AU - Bianchi Martini, Laura AU - Roselli, Maria Grazia AU - Vaghetti, Edoardo AU - Settimi, Laura AU - Orsi, Daniela AU - Battista, Giuseppe AD - UO Igiene e salute nei luoghi di lavoro, USL 2, Lucca. PY - 2005 SP - 271 EP - 277 VL - 29 IS - 5-6 SN - 1120-9763, 1120-9763 KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Sex Factors KW - Risk Factors KW - Humans KW - Cohort Studies KW - Adult KW - Middle Aged KW - Follow-Up Studies KW - Adolescent KW - Time Factors KW - Male KW - Italy KW - Female KW - Cause of Death KW - Pleural Neoplasms -- mortality KW - Mesothelioma -- etiology KW - Occupational Diseases -- etiology KW - Mesothelioma -- mortality KW - Occupational Exposure -- adverse effects KW - Asbestos -- adverse effects KW - Tobacco Industry KW - Pleural Neoplasms -- etiology KW - Occupational Diseases -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70160239?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiologia+e+prevenzione&rft.atitle=%5BMortality+among+workers+in+a+cigarette+factory+in+Lucca+%28Tuscany%29%5D.&rft.au=Parducci%2C+Dino+Ausilio%3BPuccetti%2C+Monica%3BBianchi+Martini%2C+Laura%3BRoselli%2C+Maria+Grazia%3BVaghetti%2C+Edoardo%3BSettimi%2C+Laura%3BOrsi%2C+Daniela%3BBattista%2C+Giuseppe&rft.aulast=Parducci&rft.aufirst=Dino&rft.date=2005-09-01&rft.volume=29&rft.issue=5-6&rft.spage=271&rft.isbn=&rft.btitle=&rft.title=Epidemiologia+e+prevenzione&rft.issn=11209763&rft_id=info:doi/ LA - Italian DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-31 N1 - Date created - 2006-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Metabolic effects of IGF-I deficiency: lessons from mouse models. AN - 68916750; 16369209 AB - Insulin and insulin-like growth factors (IGFs) belong to the most biologically characterized family of peptides involved in metabolism, growth and development. The cellular responses to the IGFs are mediated primarily by the IGF-I receptor. The IGF-I receptor is a member of the family of tyrosine kinase growth factor receptors, and is highly homologous (70%) to the insulin receptor, especially in the tyrosine kinase domain (84%) ADDIN. Upon ligand binding to the extracellular region, the intrinsic tyrosine kinase domain of the receptor is activated. In the past it was believed that insulin activates primarily metabolic processes while IGFs promote cell growth and differentiation. However, in the last two decades many animal models of IGFI deficiency and excess revealed the importance of IGF-I in carbohydrate and lipid metabolism and now it is clear that these peptide hormones together with growth hormone (GH) work in a coordinate and interdependent manner. In the circulation, IGFs are bound in a binary complex with a family of high affinity IGF-binding proteins (IGFBPs) ADDIN. However, most of the circulating IGF-I associates with a high molecular weight complex approximately 150 KDa consisting of IGFBP-3 and the acid labile subunit (ALS) ADDIN. Once the ternary complex dissociates, the binary complexes of IGFBP-IGFs are removed from the circulation and by crossing the endothelium to reach the target tissues and to interact with cell surface receptors. In the present review we will summarize the role of GH and IGF in somatic growth and focus on the metabolic effects of IGF-I deficiency as assessed in various mouse models. JF - Pediatric endocrinology reviews : PER AU - Yakar, Shoshana AU - Sun, Hui AU - Zhao, Hong AU - Pennisi, Patricia AU - Toyoshima, Yuka AU - Setser, Jennifer AU - Stannard, Bethel AU - Scavo, Louis AU - Leroith, Derek AD - Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. ShoshanaY@intra.niddk.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 11 EP - 19 VL - 3 IS - 1 SN - 1565-4753, 1565-4753 KW - Insulin KW - 0 KW - Human Growth Hormone KW - 12629-01-5 KW - Insulin-Like Growth Factor I KW - 67763-96-6 KW - Index Medicus KW - Animals KW - Drug Interactions KW - Liver -- metabolism KW - Mice KW - Insulin -- pharmacology KW - Mice, Transgenic KW - Metabolism KW - Mice, Knockout KW - Human Growth Hormone -- physiology KW - Human Growth Hormone -- pharmacology KW - Insulin-Like Growth Factor I -- genetics KW - Insulin-Like Growth Factor I -- physiology KW - Insulin-Like Growth Factor I -- deficiency KW - Disease Models, Animal UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68916750?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatric+endocrinology+reviews+%3A+PER&rft.atitle=Metabolic+effects+of+IGF-I+deficiency%3A+lessons+from+mouse+models.&rft.au=Yakar%2C+Shoshana%3BSun%2C+Hui%3BZhao%2C+Hong%3BPennisi%2C+Patricia%3BToyoshima%2C+Yuka%3BSetser%2C+Jennifer%3BStannard%2C+Bethel%3BScavo%2C+Louis%3BLeroith%2C+Derek&rft.aulast=Yakar&rft.aufirst=Shoshana&rft.date=2005-09-01&rft.volume=3&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Pediatric+endocrinology+reviews+%3A+PER&rft.issn=15654753&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-12 N1 - Date created - 2005-12-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The effects of the going places program on early adolescent substance use and antisocial behavior. AN - 68907519; 16091854 AB - This study evaluated the effects of a school-based intervention on growth trajectories of smoking, drinking, and antisocial behavior among early adolescents. Seven middle schools were randomized to intervention or comparison conditions and students in two successive cohorts (n = 1484) provided five waves of data from sixth to ninth grade. The Going Places Program, included classroom curricula, parent education, and school environment components. Latent growth curve analyses demonstrated significant treatment group effects, including reducing increases in friends who smoke, outcome expectations for smoking, and smoking progression, but had non-significant effects on drinking or antisocial behavior. The Going Places Program was effective in preventing increases in smoking progression, but its efficacy as a more cross-cutting problem behavior preventive intervention was not confirmed. JF - Prevention science : the official journal of the Society for Prevention Research AU - Simons-Morton, Bruce AU - Haynie, Denise AU - Saylor, Keith AU - Crump, Aria Davis AU - Chen, Rusan AD - Prevention Research Branch DESPR, NICHD, NIH, Rockville, Maryland 20852-7510, USA. Mortonb@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 187 EP - 197 VL - 6 IS - 3 SN - 1389-4986, 1389-4986 KW - Index Medicus KW - Humans KW - Curriculum KW - Maryland KW - Adolescent KW - Male KW - Female KW - Schools KW - Antisocial Personality Disorder -- prevention & control KW - Substance-Related Disorders -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68907519?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prevention+science+%3A+the+official+journal+of+the+Society+for+Prevention+Research&rft.atitle=The+effects+of+the+going+places+program+on+early+adolescent+substance+use+and+antisocial+behavior.&rft.au=Simons-Morton%2C+Bruce%3BHaynie%2C+Denise%3BSaylor%2C+Keith%3BCrump%2C+Aria+Davis%3BChen%2C+Rusan&rft.aulast=Simons-Morton&rft.aufirst=Bruce&rft.date=2005-09-01&rft.volume=6&rft.issue=3&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Prevention+science+%3A+the+official+journal+of+the+Society+for+Prevention+Research&rft.issn=13894986&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-14 N1 - Date created - 2005-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Enhancement of hidden structures of early skin fibrosis using polarization degree patterns and Pearson correlation analysis. AN - 68811346; 16292958 AB - The skin of athymic nude mice is irradiated with a single dose of x-ray irradiation that initiated fibrosis. Digital photographs of the irradiated mice are taken by illuminating the mouse skin with linearly polarized probe light of 650 nm. The specific pattern of the surface distribution of the degree of polarization enables the detection of initial skin fibrosis structures that were not visually apparent. Data processing of the raw spatial distributions of the degree of polarization based on Fourier filtering of the high-frequency noise improves subjective perception of the revealed structure in the images. In addition, Pearson correlation analysis provides information about skin structural size and directionality. JF - Journal of biomedical optics AU - Sviridov, Alexander P AU - Chernomordik, Victor AU - Hassan, Moinuddin AU - Boccara, Albert C AU - Russo, Angelo AU - Smith, Paul AU - Gandjbakhche, Amir AD - National Institutes of Health, Bethesda, Maryland 20892, USA. PY - 2005 SP - 051706 VL - 10 IS - 5 SN - 1083-3668, 1083-3668 KW - Index Medicus KW - Sensitivity and Specificity KW - Animals KW - X-Rays KW - Reproducibility of Results KW - Fibrosis KW - Mice, Nude KW - Mice KW - Statistics as Topic KW - Fourier Analysis KW - Image Interpretation, Computer-Assisted -- methods KW - Signal Processing, Computer-Assisted KW - Radiodermatitis -- pathology KW - Algorithms KW - Image Enhancement -- methods KW - Microscopy, Polarization -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68811346?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+biomedical+optics&rft.atitle=Enhancement+of+hidden+structures+of+early+skin+fibrosis+using+polarization+degree+patterns+and+Pearson+correlation+analysis.&rft.au=Sviridov%2C+Alexander+P%3BChernomordik%2C+Victor%3BHassan%2C+Moinuddin%3BBoccara%2C+Albert+C%3BRusso%2C+Angelo%3BSmith%2C+Paul%3BGandjbakhche%2C+Amir&rft.aulast=Sviridov&rft.aufirst=Alexander&rft.date=2005-09-01&rft.volume=10&rft.issue=5&rft.spage=051706&rft.isbn=&rft.btitle=&rft.title=Journal+of+biomedical+optics&rft.issn=10833668&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-04-04 N1 - Date created - 2005-11-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Overexpression of 15-lipoxygenase-1 induces growth arrest through phosphorylation of p53 in human colorectal cancer cells. AN - 68616244; 16179498 AB - To investigate the function of 15-lipoxygenase-1 (15-LOX-1) in human colorectal cancer, we overexpressed 15-LOX-1 in HCT-116 human colorectal cancer cells. Clones expressing the highest levels of 15-LOX-1 displayed reduced viability compared with the HCT-116-Vector control cells. Further, by cell cycle gene array analyses, the cyclin-dependent kinase inhibitor p21WAF1/CIP1 and MDM2 genes were up-regulated in 15-LOX-1-overexpressing cells. The induction of p21(WAF1/CIP1) and MDM2 were linked to activation of p53 by 15-LOX-1, as there was a dramatic induction of phosphorylated p53 (Ser15) in 15-LOX-1-overesxpressing cells. However, the 15-LOX-1 metabolites 13(S)-hydroxyoctadecadienoic acid and 15(S)-hydroxyeicosatetraenoic acid failed to induce phosphorylation of p53 at Ser15, and the 15-LOX-1 inhibitor PD146176 did not inhibit the phosphorylation of p53 at Ser15 in 15-LOX-1-overexpressing cells. Nonetheless, the growth-inhibitory effects of 15-LOX-1 were p53 dependent, as 15-LOX-1 overexpression had no effect on cell growth in p53 (-/-) HCT-116 cells. Finally, treatment of HCT-116-15-LOX-1 cells with different kinase inhibitors suggested that the effects of 15-LOX-1 on p53 phosphorylation and activation were due to effects on DNA-dependent protein kinase. Collectively, these findings suggest a new mechanism to explain the biological activity of 15-LOX-1, where 15-LOX plays a stoichiometric role in activating a DNA-dependent protein kinase-dependent pathway that leads to p53-dependent growth arrest. JF - Molecular cancer research : MCR AU - Kim, Jong-Sik AU - Baek, Seung Joon AU - Bottone, Frank G AU - Sali, Tina AU - Eling, Thomas E AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, MD, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 511 EP - 517 VL - 3 IS - 9 SN - 1541-7786, 1541-7786 KW - CDKN1A protein, human KW - 0 KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - DNA-Binding Proteins KW - Linoleic Acids KW - Lipoxygenase Inhibitors KW - Nuclear Proteins KW - Proto-Oncogene Proteins KW - Tumor Suppressor Protein p53 KW - 13-hydroxy-9,11-octadecadienoic acid KW - 5204-88-6 KW - 15-hydroxy-5,8,11,13,17-eicosapentaenoic acid KW - 97850-14-1 KW - Eicosapentaenoic Acid KW - AAN7QOV9EA KW - Arachidonate 15-Lipoxygenase KW - EC 1.13.11.33 KW - MDM2 protein, human KW - EC 2.3.2.27 KW - Proto-Oncogene Proteins c-mdm2 KW - DNA-Activated Protein Kinase KW - EC 2.7.11.1 KW - PRKDC protein, human KW - Protein-Serine-Threonine Kinases KW - Index Medicus KW - Humans KW - DNA-Binding Proteins -- pharmacology KW - Proto-Oncogene Proteins -- metabolism KW - Eicosapentaenoic Acid -- analogs & derivatives KW - HCT116 Cells KW - Cell Cycle Proteins -- metabolism KW - Gene Expression Regulation, Neoplastic KW - Protein-Serine-Threonine Kinases -- pharmacology KW - Tumor Cells, Cultured KW - Phosphorylation KW - Lipoxygenase Inhibitors -- pharmacology KW - Linoleic Acids -- metabolism KW - Nuclear Proteins -- metabolism KW - Eicosapentaenoic Acid -- metabolism KW - Cell Division KW - Arachidonate 15-Lipoxygenase -- metabolism KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68616244?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+research+%3A+MCR&rft.atitle=Overexpression+of+15-lipoxygenase-1+induces+growth+arrest+through+phosphorylation+of+p53+in+human+colorectal+cancer+cells.&rft.au=Kim%2C+Jong-Sik%3BBaek%2C+Seung+Joon%3BBottone%2C+Frank+G%3BSali%2C+Tina%3BEling%2C+Thomas+E&rft.aulast=Kim&rft.aufirst=Jong-Sik&rft.date=2005-09-01&rft.volume=3&rft.issue=9&rft.spage=511&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+research+%3A+MCR&rft.issn=15417786&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-10 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In islet-specific glucose-6-phosphatase-related protein, the beta cell antigenic sequence that is targeted in diabetes is not responsible for the loss of phosphohydrolase activity. AN - 68586139; 16012821 AB - There are three members of the glucose-6-phosphatase (G6Pase) family: (1) the liver/kidney/intestine G6Pase-alpha (encoded by G6PC), which is a key enzyme in glucose homeostasis; (2) the ubiquitous G6Pase-beta (encoded by G6PC3); and (3) the islet-specific G6Pase-related protein (IGRP, encoded by /G6PC2). While G6Pase-alpha and G6Pase-beta are functional glucose-6-phosphate hydrolases, IGRP possesses almost no hydrolase activity. This was unexpected since G6Pase-alpha is more closely related to IGRP than G6Pase-beta. Recently, amino acids 206-214 in IGRP were identified as a beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes, suggesting that this peptide confers functional specificity to IGRP. We therefore investigated the molecular events that inactivate IGRP activity and the effects of the beta cell antigen sequence on the stability and enzymatic activity of G6Pase-alpha. Studies were performed using site-directed mutagenesis and transient expression assays. Protein stability was evaluated by Western blotting, proteasome inhibitor studies and in vitro transcription-translation. We showed that the residues responsible for G6Pase activity are more extensive than previously recognised. Introducing the IGRP antigenic motif into G6Pase-alpha does not completely destroy activity, although it does destabilise the protein. The low hydrolytic activity in IGRP is due to the combination of multiple independent mutations. The loss of catalytic activity in IGRP arises from the sum of many sequence differences. G6Pase-alpha mutants containing the beta cell antigen sequence are preferentially degraded in cells, which prevents targeting by pathogenic CD8+ T cells. It is possible that IGRP levels in beta cells could dictate susceptibilities to diabetes. JF - Diabetologia AU - Shieh, J-J AU - Pan, C-J AU - Mansfield, B C AU - Chou, J Y AD - Section on Cellular Differentiation, Heritable Disorders Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1830, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1851 EP - 1859 VL - 48 IS - 9 SN - 0012-186X, 0012-186X KW - DNA Primers KW - 0 KW - Phosphoric Monoester Hydrolases KW - EC 3.1.3.2 KW - Glucose-6-Phosphatase KW - EC 3.1.3.9 KW - G6PC2 protein, human KW - EC 3.1.3.9. KW - Index Medicus KW - Animals KW - COS Cells KW - Humans KW - Amino Acid Sequence KW - Mice KW - Cloning, Molecular KW - Rats KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Sequence Alignment KW - Conserved Sequence KW - Cercopithecus aethiops KW - Molecular Sequence Data KW - Dogs KW - Sequence Homology, Amino Acid KW - Amino Acid Substitution KW - Glucose-6-Phosphatase -- chemistry KW - Glucose-6-Phosphatase -- physiology KW - Islets of Langerhans -- physiology KW - Islets of Langerhans -- enzymology KW - Phosphoric Monoester Hydrolases -- metabolism KW - Glucose-6-Phosphatase -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68586139?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diabetologia&rft.atitle=In+islet-specific+glucose-6-phosphatase-related+protein%2C+the+beta+cell+antigenic+sequence+that+is+targeted+in+diabetes+is+not+responsible+for+the+loss+of+phosphohydrolase+activity.&rft.au=Shieh%2C+J-J%3BPan%2C+C-J%3BMansfield%2C+B+C%3BChou%2C+J+Y&rft.aulast=Shieh&rft.aufirst=J-J&rft.date=2005-09-01&rft.volume=48&rft.issue=9&rft.spage=1851&rft.isbn=&rft.btitle=&rft.title=Diabetologia&rft.issn=0012186X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-03 N1 - Date created - 2005-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevention of alcohol-induced developmental delays and learning abnormalities in a model of fetal alcohol syndrome. AN - 68581133; 16157106 AB - Prenatal alcohol exposure results in fetal death and neurobehavioral complications including learning impairment. Previously synthetic peptides derived from activity-dependent neurotrophic factor have been shown to prevent aspects of alcohol-induced damage in pregnancy. The objective of this work was to evaluate whether activity-dependent neurotrophic factor-12 could prevent alcohol-induced damage in a model of fetal alcohol syndrome. Using a well-characterized model, C57Bl6/J mice on gestational day 8 were treated with placebo, alcohol (30% volume/volume alcohol 0.03 mL/kg), alcohol plus activity-dependent neurotrophic factor-12 30 minutes prior to alcohol, or activity-dependent neurotrophic factor-12 alone. Fetal death was assessed on gestational day 18 (25 litters were evaluated: alcohol, n = 5; placebo, n = 9; alcohol plus activity-dependent neurotrophic factor-12, n = 11). Neonatal behavior tests were performed on postnatal days 1 through 21 with the offspring of 12 dams (alcohol, n = 16; placebo, n = 46; alcohol plus activity-dependent neurotrophic factor-12, n = 23; and activity-dependent neurotrophic factor-12, n = 35). Adult males were tested in the Morris water maze for learning assessment and with the hole punch activity test for exploratory activity. Statistical analysis included Kruskal-Wallis and analysis of variance. Fetal death was greater in alcohol (67% +/- 13%) vs placebo (8.4% +/- 3%, P .5) and significantly better than alcohol on days 4, 6, and 7 (all P < .05). Alcohol exposure resulted in significantly less time in hole punch activity (P < .02) than control. Activity-dependent neurotrophic factor-12 pretreatment prevented the alcohol-induced decline, with levels the same as control (P = .1). The novel peptide activity-dependent neurotrophic factor-12 prevents alcohol-induced fetal death and developmental and learning abnormalities in a model of fetal alcohol syndrome. This demonstrates that a single treatment with a peptide is efficacious and may be of value in the prevention of alcohol-induced damage. JF - American journal of obstetrics and gynecology AU - Endres, M AU - Toso, L AU - Roberson, R AU - Park, J AU - Abebe, D AU - Poggi, S AU - Spong, C Y AD - Unit on Perinatal and Developmental Neurobiology, National Insitute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-0925, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1028 EP - 1034 VL - 193 IS - 3 Pt 2 SN - 0002-9378, 0002-9378 KW - Nerve Tissue Proteins KW - 0 KW - Ethanol KW - 3K9958V90M KW - Abridged Index Medicus KW - Index Medicus KW - Mice, Inbred Strains KW - Fetal Death -- prevention & control KW - Animals KW - Cognition -- drug effects KW - Motor Activity KW - Learning -- drug effects KW - Disease Models, Animal KW - Mice KW - Fetal Growth Retardation -- prevention & control KW - Female KW - Pregnancy KW - Maze Learning KW - Fetal Alcohol Spectrum Disorders -- physiopathology KW - Ethanol -- administration & dosage KW - Nerve Tissue Proteins -- therapeutic use KW - Learning Disorders -- prevention & control KW - Learning Disorders -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68581133?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+obstetrics+and+gynecology&rft.atitle=Prevention+of+alcohol-induced+developmental+delays+and+learning+abnormalities+in+a+model+of+fetal+alcohol+syndrome.&rft.au=Endres%2C+M%3BToso%2C+L%3BRoberson%2C+R%3BPark%2C+J%3BAbebe%2C+D%3BPoggi%2C+S%3BSpong%2C+C+Y&rft.aulast=Endres&rft.aufirst=M&rft.date=2005-09-01&rft.volume=193&rft.issue=3+Pt+2&rft.spage=1028&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=00029378&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Interdisciplinary neurotoxicity inhalation studies: carbon disulfide and carbonyl sulfide research in F344 rats. AN - 68576298; 16002115 AB - Inhalation studies were conducted on the hazardous air pollutants, carbon disulfide, which targets the central nervous system (spinal cord) and peripheral nervous system (distal portions of long myelinated axons), and carbonyl sulfide, which targets the central nervous system (brain). The objectives were to investigate the neurotoxicity of these compounds by a comprehensive evaluation of function, structure, and mechanisms of disease. Through interdisciplinary research, the major finding in the carbon disulfide inhalation studies was that carbon disulfide produced intra- and intermolecular protein cross-linking in vivo. The observation of dose-dependent covalent cross-linking in neurofilament proteins prior to the onset of lesions is consistent with this process contributing to the development of the neurofilamentous axonal swellings characteristic of carbon disulfide neurotoxicity. Of significance is that valine-lysine thiourea cross-linking on rat globin and lysine-lysine thiourea cross-linking on erythrocyte spectrin reflect cross-linking events occurring within the axon and could potentially serve as biomarkers of carbon disulfide exposure and effect. In the carbonyl sulfide studies, using magnetic resonance microscopy (MRM), we determined that carbonyl sulfide targets the auditory pathway in the brain. MRM allowed the examination of 200 brain slices and made it possible to identify the most vulnerable sites of neurotoxicity, which would have been missed in our traditional neuropathology evaluations. Electrophysiological studies were focused on the auditory system and demonstrated decreases in auditory brain stem evoked responses. Similarly, mechanistic studies focused on evaluating cytochrome oxidase activity in the posterior colliculus and parietal cortex. A decrease in cytochrome oxidase activity was considered to be a contributing factor to the pathogenesis of carbonyl sulfide neurotoxicity. JF - Toxicology and applied pharmacology AU - Sills, Robert C AU - Harry, G Jean AU - Valentine, William M AU - Morgan, Daniel L AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, 111 Alexander Drive, South Campus, MD B3-08, PO Box 12233, Research Triangle Park, NC 27709, USA. sills@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 245 EP - 250 VL - 207 IS - 2 Suppl KW - Sulfur Oxides KW - 0 KW - carbonyl sulfide KW - 871UI0ET21 KW - Carbon Disulfide KW - S54S8B99E8 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Rats, Inbred F344 KW - Administration, Inhalation KW - Carbon Disulfide -- toxicity KW - Nervous System -- drug effects KW - Sulfur Oxides -- administration & dosage KW - Sulfur Oxides -- toxicity KW - Carbon Disulfide -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68576298?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Interdisciplinary+neurotoxicity+inhalation+studies%3A+carbon+disulfide+and+carbonyl+sulfide+research+in+F344+rats.&rft.au=Sills%2C+Robert+C%3BHarry%2C+G+Jean%3BValentine%2C+William+M%3BMorgan%2C+Daniel+L&rft.aulast=Sills&rft.aufirst=Robert&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=245&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Complementary gene and protein expression studies and integrative approaches in toxicogenomics. AN - 68576161; 15992845 AB - Parallel transcript and protein profiling is a strategy to gain further insight into the mechanisms of toxicity and disease. The technologies used to measure expression at the transcript and protein levels each convey different information and have different technical capabilities that can complement each other when combined. In this review, over twenty studies are considered for the use of -Omics platform, the chemical or disease being profiled, tissues, the number of genes and proteins found by each platform and common expression products. A strategy is suggested for toxicant expression profiling that combines the transcriptomics and proteomics of both the target tissue and blood/serum that could provide a more complete characterization of toxicity as well as synergize expression technologies toward biomarker discovery. JF - Toxicology and applied pharmacology AU - Merrick, B Alex AU - Madenspacher, Jennifer H AD - National Center for Toxicogenomics, National Institute of Environmental Health Sciences, D2-04, P.O. Box 12233, Research Triangle Park, NC 27709, USA. merrick@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 189 EP - 194 VL - 207 IS - 2 Suppl KW - Index Medicus KW - Animals KW - Humans KW - Gene Expression Profiling KW - Proteomics KW - Toxicology KW - Genomics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68576161?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Complementary+gene+and+protein+expression+studies+and+integrative+approaches+in+toxicogenomics.&rft.au=Merrick%2C+B+Alex%3BMadenspacher%2C+Jennifer+H&rft.aulast=Merrick&rft.aufirst=B&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=189&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene-particulate matter-health interactions. AN - 68575289; 15979667 AB - Inter-individual variation in human responses to air pollutants suggests that some subpopulations are at increased risk to the detrimental effects of pollutant exposure. Extrinsic factors such as previous exposure and nutritional status may influence individual susceptibility. Intrinsic (host) factors that determine susceptibility include age, gender, and pre-existing disease (e.g., asthma), and it is becoming clear that genetic background also contributes to individual susceptibility. Environmental exposures to particulates and genetic factors associated with disease risk likely interact in a complex fashion that varies from one population and one individual to another. The relationships between genetic background and disease risk and severity are often evaluated through traditional family-based linkage studies and positional cloning techniques. However, case-control studies based on association of disease or disease subphenotypes with candidate genes have advantages over family pedigree studies for complex disease phenotypes. This is based in part on continued development of quantitative analysis and the discovery and availability of simple sequence repeats and single nucleotide polymorphisms. Linkage analyses with genetically standardized animal models also provide a useful tool to identify genetic determinants of responses to environmental pollutants. These approaches have identified significant susceptibility quantitative trait loci on mouse chromosomes 1, 6, 11, and 17. Physical mapping and comparative mapping between human and mouse genomes will yield candidate susceptibility genes that may be tested by association studies in human subjects. Human studies and mouse modeling will provide important insight to understanding genetic factors that contribute to differential susceptibility to air pollutants. JF - Toxicology and applied pharmacology AU - Kleeberger, Steven R AU - Ohtsuka, Yoshinori AD - Laboratory of Respiratory Biology, Environmental Genetics Group, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Drive, Research Triangle Park, NC 27705, USA. kleeber1@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 276 EP - 281 VL - 207 IS - 2 Suppl KW - Air Pollutants KW - 0 KW - Index Medicus KW - Genetic Linkage KW - Animals KW - Polymorphism, Single Nucleotide KW - Particle Size KW - Humans KW - Case-Control Studies KW - Repetitive Sequences, Nucleic Acid KW - Air Pollutants -- toxicity KW - Genetics, Medical UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68575289?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Gene-particulate+matter-health+interactions.&rft.au=Kleeberger%2C+Steven+R%3BOhtsuka%2C+Yoshinori&rft.aulast=Kleeberger&rft.aufirst=Steven&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=276&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dustborne Alternaria alternata antigens in US homes: results from the National Survey of Lead and Allergens in Housing. AN - 68573555; 16159634 AB - Alternaria alternata is one of the most common fungi associated with allergic disease. However, Alternaria exposure in indoor environments is not well characterized. The primary goals of this study were to examine the prevalence of Alternaria exposure and identify independent predictors of Alternaria antigen concentrations in US homes. Data for this cross-sectional study were obtained from the National Survey of Lead and Allergens in Housing. A nationally representative sample of 831 housing units in 75 different locations throughout the United States completed the survey. Information on housing and household characteristics was obtained by questionnaire and environmental assessments. Concentrations of A alternata antigens in dust collected from various indoor sites were assessed with a polyclonal anti-Alternaria antibody assay. Alternaria antigens were detected in most (95% to 99%) of the dust samples. The geometric mean concentration, reflecting the average Alternaria concentration in homes, was 4.88 microg/g (SEM, 0.13 microg/g). In the multivariable linear regression analysis, the age of the housing unit, geographic region, urbanization, poverty, family race, observed mold and moisture problems, use of de-humidifier, and presence of cats and dogs were independent predictors of Alternaria antigen concentrations. Less frequent cleaning and smoking indoors also contributed to higher Alternaria antigen levels in homes. Exposure to A alternata antigens in US homes is common. Antigen levels in homes are influenced not only by regional factors but also by residential characteristics. Preventing mold and moisture problems, avoiding smoking indoors, and regular household cleaning may help reduce exposure to Alternaria antigens indoors. JF - The Journal of allergy and clinical immunology AU - Salo, Päivi M AU - Yin, Ming AU - Arbes, Samuel J AU - Cohn, Richard D AU - Sever, Michelle AU - Muilenberg, Michael AU - Burge, Harriet A AU - London, Stephanie J AU - Zeldin, Darryl C AD - National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 623 EP - 629 VL - 116 IS - 3 SN - 0091-6749, 0091-6749 KW - Antigens, Fungal KW - 0 KW - Dust KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Cross-Sectional Studies KW - Animals KW - Housing KW - Humans KW - Humidity KW - Prevalence KW - Antigens, Fungal -- immunology KW - Air Pollution, Indoor KW - Dust -- immunology KW - Environmental Exposure KW - Alternaria -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68573555?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+allergy+and+clinical+immunology&rft.atitle=Dustborne+Alternaria+alternata+antigens+in+US+homes%3A+results+from+the+National+Survey+of+Lead+and+Allergens+in+Housing.&rft.au=Salo%2C+P%C3%A4ivi+M%3BYin%2C+Ming%3BArbes%2C+Samuel+J%3BCohn%2C+Richard+D%3BSever%2C+Michelle%3BMuilenberg%2C+Michael%3BBurge%2C+Harriet+A%3BLondon%2C+Stephanie+J%3BZeldin%2C+Darryl+C&rft.aulast=Salo&rft.aufirst=P%C3%A4ivi&rft.date=2005-09-01&rft.volume=116&rft.issue=3&rft.spage=623&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+allergy+and+clinical+immunology&rft.issn=00916749&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-15 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Allergy Clin Immunol. 2003 Feb;111(2):285-9 [12589346] Pediatr Allergy Immunol. 2003 Apr;14(2):100-5 [12675755] J Allergy Clin Immunol. 2004 Feb;113(2):227-34 [14767434] J Allergy Clin Immunol. 2004 Sep;114(3):599-606 [15356564] Ann Allergy. 1981 Jan;46(1):30-3 [7192959] J Allergy Clin Immunol. 1985 Dec;76(6):819-25 [4067131] Ann Allergy. 1990 Aug;65(2):109-14 [2382872] N Engl J Med. 1991 Feb 7;324(6):359-63 [1987459] J Allergy Clin Immunol. 1992 Mar;89(3):752-9 [1545096] J Allergy Clin Immunol. 1993 Mar;91(3):773-82 [8454800] Clin Microbiol Rev. 1995 Apr;8(2):161-79 [7621398] Am J Respir Crit Care Med. 1997 Apr;155(4):1356-61 [9105079] Clin Exp Allergy. 1998 Apr;28(4):459-67 [9641573] J Allergy Clin Immunol. 1998 Oct;102(4 Pt 1):563-70 [9802363] J Allergy Clin Immunol. 1999 Mar;103(3 Pt 1):494-500 [10069885] J Allergy Clin Immunol. 1999 Apr;103(4):709-11 [10200024] J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):665-71 [10482844] Can J Public Health. 1999 Jul-Aug;90(4):244-9 [10489721] Clin Exp Allergy. 1999 Nov;29(11):1481-9 [10520075] Acta Allergol. 1962;17:130-59 [14492419] J Expo Anal Environ Epidemiol. 1999 Nov-Dec;9(6):560-8 [10638841] Ann Allergy Asthma Immunol. 2000 Jan;84(1):47-54 [10674565] Environ Health Perspect. 2000 Aug;108 Suppl 4:653-9 [10931783] Clin Exp Allergy. 2000 Dec;30(12):1733-9 [11122211] J Allergy Clin Immunol. 2001 Feb;107(2):388-90 [11174210] J Allergy Clin Immunol. 2001 Mar;107(3 Suppl):S430-40 [11242604] J Allergy Clin Immunol. 2001 Apr;107(4):641-6 [11295652] Ann Allergy Asthma Immunol. 2001 May;86(5):517-23 [11379802] Am J Respir Crit Care Med. 2001 Aug 1;164(3):455-9 [11500349] Appl Environ Microbiol. 2002 Apr;68(4):1743-53 [11916692] Environ Health Perspect. 2002 May;110(5):527-32 [12003758] BMJ. 2002 Aug 24;325(7361):411-4 [12193354] J Expo Anal Environ Epidemiol. 2002 Nov;12(6):427-32 [12415491] Allergy. 2003 Jan;58(1):13-20 [12580801] Environ Health Perspect. 1997 Jun;105(6):622-35 [9288497] Mycopathologia. 1997;139(1):23-33 [9511234] Ann Allergy Asthma Immunol. 1998 Mar;80(3):279-85 [9532979] J Allergy Clin Immunol. 1998 May;101(5):626-32 [9600499] Comment In: J Allergy Clin Immunol. 2005 Sep;116(3):620-2 [16159633] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Novel peptides prevent alcohol-induced spatial learning deficits and proinflammatory cytokine release in a mouse model of fetal alcohol syndrome. AN - 68567818; 16150281 AB - Previously, the novel peptides NAPVSIPQ and SALLRSIPA were shown to prevent alcohol-induced fetal death and growth abnormalities in a mouse model of fetal alcohol syndrome. This study evaluated whether these peptides could prevent long-term alcohol-induced learning abnormalities. In addition, because specific cytokines are known to effect long-term potentiation, a model of learning at the molecular level, we studied the effect of these novel peptides on tumor necrosis factor-alpha, interleukin-6, and interferon-gamma levels. We used a well-characterized mouse model of fetal alcohol syndrome. Pregnant mice were injected on day 8 with alcohol (0.03 mL/kg) or placebo. Pretreatment with NAPVSIPQ+SALLRSIPA (20 mug) or placebo was given 30 minutes before alcohol. Embryos were removed after 6 hours, at which time cytokine, tumor necrosis factor-alpha, interleukin-6, and interferon-gamma levels were measured with enzyme-linked immunoassays. To test spatial learning, adult offspring from litters that were treated with alcohol, control, NAPVSIPQ+SALLRSIPA then alcohol, or NAPVSIPQ+SALLRSIPA alone were evaluated for latency to find a hidden platform in the Morris water maze. Alcohol treatment increased tumor necrosis factor-alpha levels versus control levels (50.0 +/- 3.5 pg/mL vs 32.7 +/- 2.4 pg/mL; P < .001). NAPVSIPQ+SALLRSIPA pretreatment prevented this increase (39.9 9 +/- 2.8 pg/mL; P or = 60% with no prior history of autoimmunity. Twenty-nine patients received 3 mg/kg anti-CTLA-4 every 3 weeks, whereas 27 received 3 mg/kg as their initial dose with subsequent doses reduced to 1 mg/kg every 3 weeks. In both cohorts patients received concomitant vaccination with two modified HLA-A*0201-restricted peptides from the gp100 melanoma-associated antigen, gp100:209-217(210M) and gp100:280-288(288V). Two patients achieved a complete response (ongoing at 30 and 31 months, respectively) and five patients achieved a partial response (durations of 4, 6, 25+, 26+, and 34+ months, respectively), for an overall objective response rate of 13%. Tumor regression was seen in lung, liver, brain, lymph nodes, and subcutaneous sites. Of 14 patients with grade 3/4 autoimmune toxicity, five (36%) experienced a clinical response compared with only two responses in the 42 patients (5%) with no autoimmune toxicity (P = .008). There were no significant differences in response rate or toxicity between the two dose schedules. Administration of anti-CTLA-4 monoclonal antibody plus peptide vaccination can cause durable objective responses, which correlate with the induction of autoimmunity, in patients with metastatic melanoma. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Attia, Peter AU - Phan, Giao Q AU - Maker, Ajay V AU - Robinson, Michael R AU - Quezado, Martha M AU - Yang, James C AU - Sherry, Richard M AU - Topalian, Suzanne L AU - Kammula, Udai S AU - Royal, Richard E AU - Restifo, Nicholas P AU - Haworth, Leah R AU - Levy, Catherine AU - Mavroukakis, Sharon A AU - Nichol, Geoff AU - Yellin, Michael J AU - Rosenberg, Steven A AD - Surgery Branch, National Cancer Institute, National Institutes of Health, CRC, Room 3W-3940, 10 Center Dr, Bethesda, MD 20892-1201, USA. Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 6043 EP - 6053 VL - 23 IS - 25 SN - 0732-183X, 0732-183X KW - Antigens, CD KW - 0 KW - Antigens, Differentiation KW - CTLA-4 Antigen KW - CTLA4 protein, human KW - Cancer Vaccines KW - Immunosuppressive Agents KW - Index Medicus KW - Drug Administration Schedule KW - Injections, Intravenous KW - Humans KW - Adult KW - Treatment Outcome KW - Prognosis KW - Aged KW - Middle Aged KW - Male KW - Female KW - Skin Neoplasms -- drug therapy KW - Cancer Vaccines -- immunology KW - Skin Neoplasms -- immunology KW - Antigens, Differentiation -- immunology KW - Melanoma -- drug therapy KW - Cancer Vaccines -- therapeutic use KW - Immunosuppressive Agents -- immunology KW - Autoimmunity KW - Antigens, Differentiation -- administration & dosage KW - Melanoma -- immunology KW - Immunosuppressive Agents -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68544804?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Autoimmunity+correlates+with+tumor+regression+in+patients+with+metastatic+melanoma+treated+with+anti-cytotoxic+T-lymphocyte+antigen-4.&rft.au=Attia%2C+Peter%3BPhan%2C+Giao+Q%3BMaker%2C+Ajay+V%3BRobinson%2C+Michael+R%3BQuezado%2C+Martha+M%3BYang%2C+James+C%3BSherry%2C+Richard+M%3BTopalian%2C+Suzanne+L%3BKammula%2C+Udai+S%3BRoyal%2C+Richard+E%3BRestifo%2C+Nicholas+P%3BHaworth%2C+Leah+R%3BLevy%2C+Catherine%3BMavroukakis%2C+Sharon+A%3BNichol%2C+Geoff%3BYellin%2C+Michael+J%3BRosenberg%2C+Steven+A&rft.aulast=Attia&rft.aufirst=Peter&rft.date=2005-09-01&rft.volume=23&rft.issue=25&rft.spage=6043&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-09-01 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Immunol. 1993 Oct 1;151(7):3489-99 [8397258] Immunity. 1994 Aug;1(5):405-13 [7882171] Immunity. 1994 Dec;1(9):793-801 [7534620] Science. 1995 Nov 10;270(5238):985-8 [7481803] Nature. 1987 Jul 16-22;328(6127):267-70 [3496540] Science. 1970 Sep 11;169(3950):1042-9 [4194660] J Immunol. 2004 Aug 15;173(4):2866-76 [15295006] J Immunol. 2003 Dec 1;171(11):5673-7 [14634073] Nat Rev Cancer. 2003 Sep;3(9):666-75 [12951585] J Immunol. 2002 Aug 15;169(4):1852-8 [12165509] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8372-7 [12826605] J Exp Med. 1996 Jun 1;183(6):2533-40 [8676074] J Exp Med. 1996 Jun 1;183(6):2541-50 [8676075] Immunity. 1997 Dec;7(6):885-95 [9430233] Nat Med. 1998 Mar;4(3):321-7 [9500606] Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10067-71 [9707601] Cancer J Sci Am. 1998 Sep-Oct;4(5):316-23 [9815296] Diabetes. 1999 Mar;48(3):652-7 [10078573] J Immunol. 1999 May 15;162(10):5813-20 [10229815] J Exp Med. 1999 Aug 2;190(3):355-66 [10430624] J Immunother. 2004 Nov-Dec;27(6):478-9 [15534492] J Clin Oncol. 2005 Feb 1;23(4):741-50 [15613700] Science. 1996 Mar 22;271(5256):1734-6 [8596936] J Natl Cancer Inst. 2000 Feb 2;92(3):205-16 [10655437] J Exp Med. 2000 Jul 17;192(2):303-10 [10899917] Genes Immun. 2000 Feb;1(3):170-84 [11196709] Annu Rev Immunol. 2001;19:565-94 [11244047] J Exp Med. 2001 Jun 4;193(11):1311-8 [11390438] Eur J Immunol. 2002 Feb;32(2):366-73 [11807776] Nat Rev Immunol. 2001 Dec;1(3):220-8 [11905831] Curr Opin Immunol. 2002 Jun;14(3):391-6 [11973140] Nat Immunol. 2002 Jul;3(7):611-8 [12087419] Nat Rev Immunol. 2002 Jun;2(6):389-400 [12093005] J Immunother. 2003 Jul-Aug;26(4):349-56 [12843797] Nat Immunol. 2003 Apr;4(4):337-42 [12612581] Science. 2003 Feb 14;299(5609):1057-61 [12522256] Science. 1990 Jun 15;248(4961):1349-56 [2113314] J Exp Med. 1991 Mar 1;173(3):759-62 [1847724] J Exp Med. 1991 Mar 1;173(3):721-30 [1847722] J Immunol. 1988 May 15;140(10):3324-30 [2834436] J Exp Med. 1991 Sep 1;174(3):561-9 [1714933] J Immunol. 1992 Jul 15;149(2):380-8 [1320641] Comment In: J Clin Oncol. 2005 Sep 1;23(25):5875-7 [16087939] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Maternal levels of polychlorinated biphenyls in relation to preterm and small-for-gestational-age birth. AN - 68544801; 16135940 AB - In developed countries, polychlorinated biphenyls (PCBs) are ubiquitous contaminants of the environment, including foods. Within the range of the resulting low-level exposure, associations of PCBs with lower birth weight have been observed in several studies. To examine further the association of PCBs with birth outcomes, we measured serum levels in 1034 pregnant women who were enrolled in the U.S. Collaborative Perinatal Project in 1959 to 1965 before PCB manufacturing was banned. The multivariate-adjusted odds ratio for preterm birth among those with PCB levels of >/=4 microg/L of total PCBs, compared with those with <2 microg/L, was 1.1 (95% confidence interval = 0.6-2.2); for the same exposure contrast, the odds ratio for delivering an infant who was small-for-gestational-age at birth was 1.6 (0.7-3.7). Birth weight and length of gestation were essentially unrelated to PCB level. In these data, maternal levels of PCBs during pregnancy were essentially unrelated to preterm birth, birth weight, or length of gestation. An association of PCBs with small-for-gestational-age birth was observed, but the results were inconclusive and occurred in the absence of an overall decrease in birth weight. JF - Epidemiology (Cambridge, Mass.) AU - Longnecker, Matthew P AU - Klebanoff, Mark A AU - Brock, John W AU - Guo, Xuguang AD - Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC, USA. longnecker@niehs.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 641 EP - 647 VL - 16 IS - 5 SN - 1044-3983, 1044-3983 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Prospective Studies KW - Risk Factors KW - Humans KW - Confounding Factors (Epidemiology) KW - Adult KW - Infant, Newborn KW - Infant, Premature KW - United States -- epidemiology KW - Male KW - Female KW - Prenatal Exposure Delayed Effects KW - Pregnancy Outcome KW - Pregnancy KW - Polychlorinated Biphenyls -- blood KW - Environmental Exposure -- adverse effects KW - Infant, Small for Gestational Age KW - Premature Birth UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68544801?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiology+%28Cambridge%2C+Mass.%29&rft.atitle=Maternal+levels+of+polychlorinated+biphenyls+in+relation+to+preterm+and+small-for-gestational-age+birth.&rft.au=Longnecker%2C+Matthew+P%3BKlebanoff%2C+Mark+A%3BBrock%2C+John+W%3BGuo%2C+Xuguang&rft.aulast=Longnecker&rft.aufirst=Matthew&rft.date=2005-09-01&rft.volume=16&rft.issue=5&rft.spage=641&rft.isbn=&rft.btitle=&rft.title=Epidemiology+%28Cambridge%2C+Mass.%29&rft.issn=10443983&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-09-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hair dyes and risk of glioma among Nebraska women. AN - 68535033; 16132796 AB - The etiology of brain cancer is not well understood. We conducted a population-based case-control study among 112 white women in Nebraska who were newly diagnosed with glioma between July 1988 and June 1993, and 215 controls, to identify risk factors for this disease. A 1.7-fold increased risk of glioma was observed for women who ever used hair coloring products (95% confidence interval (CI) = 1.0-2.9, 62 cases), and a 2.4-fold risk for those who used permanent hair coloring products (odds ratio (OR) = 2.4, 95% CI = 1.3-4.5, 39 cases). For women with the most aggressive form of glioma, glioblastoma multiforme, risk increased with duration of exposure to 4.9 (95% CI = 1.6-15.7, 10 cases) after 21 or more years of permanent hair coloring use. Higher risks were observed with earlier age at first use, but we did not see an exposure-response pattern with frequency of use of permanent dyes. No association was observed with use of non-permanent (sometimes called temporary or semi-permanent) hair coloring products. These suggestive findings need confirmation in future studies with larger sample sizes, fewer proxy respondents, and the ability to evaluate the effect of changes in formulations over time. JF - Cancer causes & control : CCC AU - Heineman, Ellen F AU - Ward, Mary H AU - McComb, Rodney D AU - Weisenburger, Dennis D AU - Zahm, Shelia Hoar AD - Division of Cancer Epidemiology and Genetics, NIH, DHHS, National Cancer Institute, Bethesda, MD 20892-7242, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 857 EP - 864 VL - 16 IS - 7 SN - 0957-5243, 0957-5243 KW - Hair Dyes KW - 0 KW - Index Medicus KW - Women's Health KW - Glioblastoma -- etiology KW - Risk Factors KW - Humans KW - Adult KW - Case-Control Studies KW - Middle Aged KW - Female KW - Survival Analysis KW - Risk Assessment KW - Nebraska -- epidemiology KW - Brain Neoplasms -- epidemiology KW - Glioma -- etiology KW - Glioma -- epidemiology KW - Hair Dyes -- adverse effects KW - Brain Neoplasms -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68535033?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+causes+%26+control+%3A+CCC&rft.atitle=Hair+dyes+and+risk+of+glioma+among+Nebraska+women.&rft.au=Heineman%2C+Ellen+F%3BWard%2C+Mary+H%3BMcComb%2C+Rodney+D%3BWeisenburger%2C+Dennis+D%3BZahm%2C+Shelia+Hoar&rft.aulast=Heineman&rft.aufirst=Ellen&rft.date=2005-09-01&rft.volume=16&rft.issue=7&rft.spage=857&rft.isbn=&rft.btitle=&rft.title=Cancer+causes+%26+control+%3A+CCC&rft.issn=09575243&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-07-07 N1 - Date created - 2005-08-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chemotherapy-induced neutropenia and treatment efficacy in advanced non-small-cell lung cancer: a pooled analysis of three randomised trials. AN - 68533293; 16129367 AB - Chemotherapy is the standard treatment for advanced non-small-cell lung cancer, and myelosuppression is a common side-effect. We aimed to assess whether haematological toxic effects could be a biological measure of drug activity and a marker of efficacy. We analysed data for 1265 patients who received chemotherapy (vinorelbine, gemcitabine, gemcitabine and vinorelbine, cisplatin and vinorelbine, or cisplatin and gemcitabine) within three randomised trials. Primary landmark analyses were restricted to 436 patients who received all six planned chemotherapy cycles and who were alive 180 days after randomisation. Neutropenia was categorised on the basis of worst WHO grade during chemotherapy: absent (grade 0), mild (grade 1-2), or severe (grade 3-4). All statistical analyses were stratified by treatment allocation. Analyses were repeated in the out-of-landmark group (829 patients), stratifying by treatment allocation and number of chemotherapy cycles. The primary endpoint was overall survival. In the landmark group, hazard ratios of death were 0.65 (0.46-0.93) for patients with severe neutropenia and 0.74 (0.56-0.98) for those with mild neutropenia. Median survival after the landmark time of 180 days was 31.4 weeks (95% CI 25.7-39.6) for patients without neutropenia compared with 42.0 weeks (32.7-59.7) for patients with severe neutropenia, and with 43.7 weeks (36.6-66.0) for those with mild neutropenia (severe vs mild vs no neutropenia p=0.0118). Findings were much the same for the out-of-landmark group. Neutropenia during chemotherapy is associated with increased survival of patients with advanced non-small-cell lung cancer, and its absence might be a result of underdosing. Prospective trials are needed to assess whether drug dosing guided by the occurrence of toxic effects could improve efficacy of standard regimens. JF - The Lancet. Oncology AU - Di Maio, Massimo AU - Gridelli, Cesare AU - Gallo, Ciro AU - Shepherd, Frances AU - Piantedosi, Franco Vito AU - Cigolari, Silvio AU - Manzione, Luigi AU - Illiano, Alfonso AU - Barbera, Santi AU - Robbiati, Sergio Federico AU - Frontini, Luciano AU - Piazza, Elena AU - Ianniello, Giovanni Pietro AU - Veltri, Enzo AU - Castiglione, Federico AU - Rosetti, Francesco AU - Gebbia, Vittorio AU - Seymour, Lesley AU - Chiodini, Paolo AU - Perrone, Francesco AD - National Cancer Institute, Naples, Italy. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 669 EP - 677 VL - 6 IS - 9 SN - 1470-2045, 1470-2045 KW - Antineoplastic Agents KW - 0 KW - Biomarkers KW - Index Medicus KW - Severity of Illness Index KW - Randomized Controlled Trials as Topic KW - Survival Rate KW - Dose-Response Relationship, Drug KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Italy -- epidemiology KW - Male KW - Female KW - Proportional Hazards Models KW - Neutropenia -- blood KW - Carcinoma, Non-Small-Cell Lung -- mortality KW - Antineoplastic Agents -- administration & dosage KW - Neutropenia -- epidemiology KW - Lung Neoplasms -- drug therapy KW - Drug Monitoring KW - Neutropenia -- chemically induced KW - Lung Neoplasms -- mortality KW - Neutropenia -- diagnosis KW - Carcinoma, Non-Small-Cell Lung -- drug therapy KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68533293?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Lancet.+Oncology&rft.atitle=Chemotherapy-induced+neutropenia+and+treatment+efficacy+in+advanced+non-small-cell+lung+cancer%3A+a+pooled+analysis+of+three+randomised+trials.&rft.au=Di+Maio%2C+Massimo%3BGridelli%2C+Cesare%3BGallo%2C+Ciro%3BShepherd%2C+Frances%3BPiantedosi%2C+Franco+Vito%3BCigolari%2C+Silvio%3BManzione%2C+Luigi%3BIlliano%2C+Alfonso%3BBarbera%2C+Santi%3BRobbiati%2C+Sergio+Federico%3BFrontini%2C+Luciano%3BPiazza%2C+Elena%3BIanniello%2C+Giovanni+Pietro%3BVeltri%2C+Enzo%3BCastiglione%2C+Federico%3BRosetti%2C+Francesco%3BGebbia%2C+Vittorio%3BSeymour%2C+Lesley%3BChiodini%2C+Paolo%3BPerrone%2C+Francesco&rft.aulast=Di+Maio&rft.aufirst=Massimo&rft.date=2005-09-01&rft.volume=6&rft.issue=9&rft.spage=669&rft.isbn=&rft.btitle=&rft.title=The+Lancet.+Oncology&rft.issn=14702045&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-08-30 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Lancet Oncol. 2005 Sep;6(9):637-8 [16129364] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Caveolin-1 is expressed on multipotent cells of hair follicles and might be involved in their resistance to chemotherapy. AN - 68516613; 16120134 AB - Caveolin-1 is the principal protein that composes caveolae, which are vesicular invaginations present on the plasma membrane of different cell types. Caveolae are involved in a variety of cellular functions including regulation of proliferation rate and resistance to chemotherapeutic drugs. Chemotherapy frequently induces alopecia which is reversible most probably due to the low proliferative rate of hair follicle stem cells and due to the expression of proteins which confer resistance. Using a specific animal model and immunohistochemistry, we analysed the expression of both caveolin-1 and the cell proliferation marker beta-catenin, at different stages of the hair follicle cycle, both before and after doxorubicin (DXR) -induced alopecia. Seven-week-old C57BL/6 mice were depilated in order to synchronize hair follicle cycle in the anagen phase. Chemotherapy with DXR 15 mg kg(-1) was used to induce alopecia. Control and treated mice were then sacrificed at precise time points and caveolin-1 expression in hairs at different stages of the cycle were analysed by immunohistochemistry. By double immunofluorescence, colocalization of caveolin-1 and cytokeratin-15 was confirmed in the bulge region. The state of proliferation of cells composing hair follicle was assessed by beta-catenin immunohistochemistry. Caveolin-1 was expressed by the cells of the bulge area, the multipotent compartment of the hair follicle, during all phases of growth (anagen), regression (catagen) and resting (telogen). During the anagen phases, nuclear beta-catenin labelling was not observed in bulge cells, but rather in the deeper portion of the follicle. Damaged hair follicles from DXR-treated mice presented bulge cells which still expressed caveolin-1, suggesting that this protein might play a role in their drug resistance. As expected, no beta-catenin nuclear staining was detectable in DXR-treated hair follicles, indicating the complete lack of proliferative processes. The differential localization of caveolin-1 and beta-catenin suggests that the mutually exclusive expression of these proteins is useful for correct hair regrowth, whether during the physiological cycle or after chemotherapy-induced alopecia. Expression of caveolin-1 within the multipotent cell compartment of the hair follicle can explain the resistance of bulge cells to many chemotherapeutics, suggested by the reversibility of chemotherapy-induced alopecia. JF - The British journal of dermatology AU - Selleri, S AU - Arnaboldi, F AU - Palazzo, M AU - Hussein, U AU - Balsari, A AU - Rumio, C AD - Department of Human Morphology, National Cancer Institute, Università degli Studi di Milano, Via Mangiagalli, 31, 20133 Milan, Italy. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 506 EP - 513 VL - 153 IS - 3 SN - 0007-0963, 0007-0963 KW - Antineoplastic Agents KW - 0 KW - Biomarkers KW - CTNNB1 protein, mouse KW - Cav1 protein, mouse KW - Caveolin 1 KW - Caveolins KW - Cytoskeletal Proteins KW - Trans-Activators KW - beta Catenin KW - Doxorubicin KW - 80168379AG KW - Index Medicus KW - Models, Animal KW - Animals KW - Doxorubicin -- adverse effects KW - Trans-Activators -- analysis KW - Drug Resistance KW - Immunohistochemistry -- methods KW - Mice KW - Cytoskeletal Proteins -- analysis KW - Cell Proliferation KW - Antineoplastic Agents -- adverse effects KW - Biomarkers -- analysis KW - Mice, Inbred C57BL KW - Hair Removal KW - Alopecia -- metabolism KW - Hair Follicle -- pathology KW - Caveolins -- metabolism KW - Multipotent Stem Cells -- metabolism KW - Multipotent Stem Cells -- drug effects KW - Caveolins -- analysis KW - Alopecia -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68516613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+British+journal+of+dermatology&rft.atitle=Caveolin-1+is+expressed+on+multipotent+cells+of+hair+follicles+and+might+be+involved+in+their+resistance+to+chemotherapy.&rft.au=Selleri%2C+S%3BArnaboldi%2C+F%3BPalazzo%2C+M%3BHussein%2C+U%3BBalsari%2C+A%3BRumio%2C+C&rft.aulast=Selleri&rft.aufirst=S&rft.date=2005-09-01&rft.volume=153&rft.issue=3&rft.spage=506&rft.isbn=&rft.btitle=&rft.title=The+British+journal+of+dermatology&rft.issn=00070963&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-14 N1 - Date created - 2005-08-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Exposure to chronic high glucose induces beta-cell apoptosis through decreased interaction of glucokinase with mitochondria: downregulation of glucokinase in pancreatic beta-cells. AN - 68513668; 16123348 AB - Chronic hyperglycemia is toxic to pancreatic beta-cells, impairing cellular functioning as observed in type 2 diabetes; however, the mechanism underlying beta-cell dysfunction and the resulting apoptosis via glucose toxicity are not fully characterized. Here, using MIN6N8 cells, a mouse pancreatic beta-cell line, we show that chronic exposure to high glucose increases cell death mediated by Bax oligomerization, cytochrome C release, and caspase-3 activation. During apoptosis, glucokinase (GCK) expression decreases in high-glucose-treated cells, concomitant with a decrease in cellular ATP production and insulin secretion. Moreover, exposure to a chronically high dose of glucose decreases interactions between GCK and mitochondria with an increase in Bax binding to mitochondria and cytochrome C release. These events are prevented by GCK overexpression, and phosphorylation of proapoptotic Bad proteins in GCK-overexpressing cells is prolonged compared with Neo-transfected cells. Similar results are obtained using primary islet cells. Collectively, these data demonstrate that beta-cell apoptosis from exposure to chronic high glucose occurs in relation to lowered GCK expression and reduced association with mitochondria. Our results show that this may be one mechanism by which glucose is toxic to beta-cells and suggests a novel approach to prevent and treat diabetes by manipulating Bax- and GCK-controlled signaling to promote apoptosis or proliferation. JF - Diabetes AU - Kim, Won-Ho AU - Lee, June Woo AU - Suh, Young Ho AU - Hong, Shin Hee AU - Choi, Joo Sun AU - Lim, Joo Hyun AU - Song, Ji Hyun AU - Gao, Bin AU - Jung, Myeong Ho AD - Division of Metabolic Disease, Department of Biomedical Science, National Institutes of Health, #5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 2602 EP - 2611 VL - 54 IS - 9 SN - 0012-1797, 0012-1797 KW - Cytochromes c KW - 9007-43-6 KW - Glucokinase KW - EC 2.7.1.2 KW - Glucose KW - IY9XDZ35W2 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Down-Regulation KW - Cytochromes c -- physiology KW - Cell Line, Tumor KW - Time Factors KW - Mitochondria -- physiology KW - Glucose -- physiology KW - Apoptosis -- drug effects KW - Glucokinase -- metabolism KW - Islets of Langerhans -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68513668?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diabetes&rft.atitle=Exposure+to+chronic+high+glucose+induces+beta-cell+apoptosis+through+decreased+interaction+of+glucokinase+with+mitochondria%3A+downregulation+of+glucokinase+in+pancreatic+beta-cells.&rft.au=Kim%2C+Won-Ho%3BLee%2C+June+Woo%3BSuh%2C+Young+Ho%3BHong%2C+Shin+Hee%3BChoi%2C+Joo+Sun%3BLim%2C+Joo+Hyun%3BSong%2C+Ji+Hyun%3BGao%2C+Bin%3BJung%2C+Myeong+Ho&rft.aulast=Kim&rft.aufirst=Won-Ho&rft.date=2005-09-01&rft.volume=54&rft.issue=9&rft.spage=2602&rft.isbn=&rft.btitle=&rft.title=Diabetes&rft.issn=00121797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-21 N1 - Date created - 2005-08-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lineage-specific engraftment and outcomes after T-cell-depleted peripheral blood stem cell transplant with Flu/Cy/TBI conditioning. AN - 68510274; 16115130 AB - Sixty patients with haematological malignancies received a myeloablative regimen of total body irradiation, cyclophosphamide and fludarabine followed by a T-cell-depleted peripheral blood stem cell transplant from a human leucocyte antigen identical sibling. To improve donor immune function, 1 x 10(7) CD3+ cells/kg were added-back between d 45 and 100. T-cell and myeloid chimaerism were monitored regularly to evaluate the effect of T-cell chimaerism on outcome. The major factor affecting outcome was disease risk, with significantly lower relapse and higher survival in 29 standard risk (SR) patients compared with 31 patients at high risk (HR) for treatment failure (relapse 4.8 +/- 5% vs. 59 +/- 11%, P or = 95% within 14 d of transplant, but in the first several months, donor T-cell chimaerism was frequently mixed. Full T-cell chimaerism was significantly more frequent in HR vs. SR patients. Landmark analysis at days 30 and 90 in HR patients with mixed versus full T-cell chimaerism, showed relapse probabilities of 50.5 +/- 14% vs. 70 +/- 16% (P = 0.62) and 34.4 +/- 20% vs. 58.8 +/- 15% (P = 0.32) respectively. Early full T-cell engraftment correlated with development of severe acute graft-versus-host disease (GVHD). However, mixed T-cell chimaerism was favourable for reducing GVHD, and did not affect relapse in this small series. JF - British journal of haematology AU - Montero, Aldemar AU - Savani, Bipin N AU - Kurlander, Roger AU - Read, Elizabeth J AU - Leitman, Susan F AU - Childs, Richard AU - Solomon, Scott R AU - Barrett, A John AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1202, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 733 EP - 739 VL - 130 IS - 5 SN - 0007-1048, 0007-1048 KW - Immunosuppressive Agents KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Vidarabine KW - FA2DM6879K KW - fludarabine KW - P2K93U8740 KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Vidarabine -- analogs & derivatives KW - Transplantation Conditioning KW - Humans KW - Transplantation Chimera KW - Child KW - Vidarabine -- administration & dosage KW - Survival Rate KW - Lymphocyte Transfusion KW - Adult KW - Graft vs Host Disease KW - Follow-Up Studies KW - Adolescent KW - Female KW - Male KW - Proportional Hazards Models KW - Hematologic Neoplasms -- therapy KW - Whole-Body Irradiation KW - Peripheral Blood Stem Cell Transplantation KW - Lymphocyte Depletion KW - Hematologic Neoplasms -- immunology KW - Immunosuppressive Agents -- therapeutic use KW - T-Lymphocytes KW - Hematologic Neoplasms -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68510274?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+journal+of+haematology&rft.atitle=Lineage-specific+engraftment+and+outcomes+after+T-cell-depleted+peripheral+blood+stem+cell+transplant+with+Flu%2FCy%2FTBI+conditioning.&rft.au=Montero%2C+Aldemar%3BSavani%2C+Bipin+N%3BKurlander%2C+Roger%3BRead%2C+Elizabeth+J%3BLeitman%2C+Susan+F%3BChilds%2C+Richard%3BSolomon%2C+Scott+R%3BBarrett%2C+A+John&rft.aulast=Montero&rft.aufirst=Aldemar&rft.date=2005-09-01&rft.volume=130&rft.issue=5&rft.spage=733&rft.isbn=&rft.btitle=&rft.title=British+journal+of+haematology&rft.issn=00071048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-08-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - (+/-)-3,4-Methylenedioxymethamphetamine administration to rats does not decrease levels of the serotonin transporter protein or alter its distribution between endosomes and the plasma membrane. AN - 68503741; 15937150 AB - We showed that the serotonin (5-HT) neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) reduces brain tissue 5-HT, decreases expression of 5-HT transporter (SERT) protein, and increases expression of glial fibrillary acidic protein (GFAP). In contrast, doses of (+/-)-3,4-methylenedioxymethamphetamine (MDMA) that decrease brain tissue 5-HT fail to alter expression of SERT or GFAP. Using a new and highly sensitive anti-SERT antibody, we determined whether MDMA alters the subcellular distribution of SERT protein by measuring SERT expression in endosomes and plasma membranes 2 weeks after MDMA administration. Rat brain tissues (caudate, cortex, and hippocampus) were collected 3 days and 2 weeks after MDMA (7.5 mg/kg i.p., every 2 h x 3 doses) or 5,7-DHT (150 microg/rat i.c.v.) administration. Representative results from cortex are as follows. At both 3 days and 2 weeks postinjection, MDMA decreased tissue 5-HT (65%) and had no effect on GFAP expression. MDMA increased heat shock protein 32 (HSP32; a marker for microglial activation) expression (30%) at 3 days, but not 2 weeks. MDMA did not alter SERT expression at either time point and did not alter SERT levels in either endosomes or plasma membranes (2 weeks). 5,7-DHT decreased tissue 5-HT (80%), increased HSP32 expression at both time points (about 50%), and increased GFAP expression at 2 weeks (40%). 5,7-DHT decreased SERT expression (33%) at 2 weeks, but not at 3 days. These findings indicate that a dosing regimen of MDMA that depletes brain 5-HT does not alter SERT protein expression or the distribution of SERT between endosomes and the plasma membrane and does not produce detectable evidence for neurotoxicity. JF - The Journal of pharmacology and experimental therapeutics AU - Wang, Xiaoying AU - Baumann, Michael H AU - Xu, Heng AU - Morales, Marisela AU - Rothman, Richard B AD - Clinical Psychopharmacology Section, Intramural Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1002 EP - 1012 VL - 314 IS - 3 SN - 0022-3565, 0022-3565 KW - Glial Fibrillary Acidic Protein KW - 0 KW - Membrane Glycoproteins KW - Membrane Transport Proteins KW - Nerve Tissue Proteins KW - Serotonin Plasma Membrane Transport Proteins KW - Slc6a4 protein, rat KW - 5,7-Dihydroxytryptamine KW - 31363-74-3 KW - Serotonin KW - 333DO1RDJY KW - Heme Oxygenase (Decyclizing) KW - EC 1.14.14.18 KW - Hmox1 protein, rat KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Index Medicus KW - Rats KW - Heme Oxygenase (Decyclizing) -- analysis KW - Animals KW - Rats, Sprague-Dawley KW - Serotonin -- analysis KW - Neurons -- chemistry KW - Neuroglia -- chemistry KW - Glial Fibrillary Acidic Protein -- analysis KW - 5,7-Dihydroxytryptamine -- pharmacology KW - Male KW - Nerve Tissue Proteins -- analysis KW - Nerve Tissue Proteins -- drug effects KW - Membrane Glycoproteins -- drug effects KW - Nerve Tissue Proteins -- metabolism KW - Membrane Transport Proteins -- drug effects KW - Membrane Glycoproteins -- analysis KW - Endosomes -- chemistry KW - Cell Membrane -- chemistry KW - N-Methyl-3,4-methylenedioxyamphetamine -- pharmacology KW - Membrane Transport Proteins -- metabolism KW - Membrane Transport Proteins -- analysis KW - Membrane Glycoproteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68503741?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Analyses+of+Recombinant+Vaccinia+and+Fowlpox+Vaccine+Vectors+Expressing+Transgenes+for+Two+Human+Tumor+Antigens+and+Three+Human+Costimulatory+Molecules&rft.au=Tsang%2C+Kwong+Y%3BPalena%2C+Claudia%3BYokokawa%2C+Junko%3BArlen%2C+Philip+M%3BGulley%2C+James+L%3BMazzara%2C+Gail+P%3BGritz%2C+Linda%3BGomez+Yafal%2C+Alicia%3BOgueta%2C+Sandra%3BGreenhalgh%2C+Patricia%3BManson%2C+Kelledy%3BPanicali%2C+Dennis%3BSchlom%2C+Jeffrey&rft.aulast=Tsang&rft.aufirst=Kwong&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1597&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-21 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The dopamine D3 receptor and drug dependence: effects on reward or beyond? AN - 68501929; 15963538 AB - Abused drugs (alcohol, heroin, cocaine, tetrahydrocannabinol and nicotine) elicit a variety of chronically relapsing disorders by interacting with brain reward systems. All of these drugs increase dopamine levels in the shell of nucleus accumbens, a structure that has been involved in their hedonic and reinforcing properties. Dopamine D(3) receptors (DRD3) are predominantly expressed in the nucleus accumbens, but also in the ventral tegmental area and in the amygdala, brain structures implicated in drug dependence. Moreover, converging pharmacological, human post-mortem and genetic studies have suggested the involvement of the DRD3 in drug dependence. Based on early studies using non-selective DRD3 ligands, the DRD3 was proposed as having a direct role in the rewarding effects of psychostimulants. However, recent studies using highly selective DRD3 ligands and the DRD3-deficient mice have revealed that the DRD3 is not implicated in the direct reinforcing effects of drugs of abuse. In contrast, the DRD3 appears to be implicated in the motivation to self-administer drugs under schedules where the response requirements are high. This is consistent with a behavioral economic analysis, with the effects of DRD3 ligands revealed only in situations with high prices for drug. Drug-self administration and relapse are strongly controlled by environmental stimuli. The DRD3 strongly modulates the influence of these environmental stimuli on drug-seeking behavior. DRD3 blockade disrupts the reactivity to drug-associated stimuli in various paradigms, such as second-order schedules of drug-self administration, conditioned place preference and Pavlovian conditioning procedures. In several paradigms, the involvement of the DRD3 has been confirmed by using DRD3-deficient mice. On the contrary, reactivity to stimuli associated with natural reinforcers, such as food, appears unaffected by modulation of the DRD3. All these findings suggest that DRD3 ligands may represent a useful strategy for decreasing relapse in abstinent drug-abusers. JF - Neuropharmacology AU - Le Foll, Bernard AU - Goldberg, Steven R AU - Sokoloff, Pierre AD - Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, National Institutes of Health/DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. blefoll@intra.nida.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 525 EP - 541 VL - 49 IS - 4 SN - 0028-3908, 0028-3908 KW - Dopamine Agents KW - 0 KW - Receptors, Dopamine D3 KW - Index Medicus KW - Dopamine Agents -- therapeutic use KW - Animals KW - Humans KW - Dopamine Agents -- pharmacology KW - Brain -- anatomy & histology KW - Gene Expression Regulation -- drug effects KW - Brain -- metabolism KW - Substance-Related Disorders -- physiopathology KW - Receptors, Dopamine D3 -- physiology KW - Reward KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68501929?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropharmacology&rft.atitle=The+dopamine+D3+receptor+and+drug+dependence%3A+effects+on+reward+or+beyond%3F&rft.au=Le+Foll%2C+Bernard%3BGoldberg%2C+Steven+R%3BSokoloff%2C+Pierre&rft.aulast=Le+Foll&rft.aufirst=Bernard&rft.date=2005-09-01&rft.volume=49&rft.issue=4&rft.spage=525&rft.isbn=&rft.btitle=&rft.title=Neuropharmacology&rft.issn=00283908&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hormonal correlates of clozapine-induced weight gain in psychotic children: an exploratory study. AN - 68496232; 16113621 AB - Weight gain is a serious side effect of atypical antipsychotics, especially in childhood. In this study, the authors examined six weight gain-related hormones in patients with childhood-onset schizophrenia (COS) after 6 weeks of clozapine treatment. Fasting serum samples for 24 patients with COS and 21 matched healthy controls (HC) were obtained. Levels of leptin, insulin, adiponectin, amylin, ghrelin, and tumor necrosis factor alpha were measured and compared between the groups. For 23 patients with COS, hormonal levels were measured at background and week 6 of clozapine treatment. Change in body mass index was correlated with levels of clozapine and changes in hormonal levels and clinical ratings. At baseline, COS did not differ significantly from HC on any hormonal measure. Clozapine treatment was associated with significant (7.9% +/- 8.5%) increase in mean body mass index. Only leptin levels increased significantly from baseline to week 6 on clozapine (p = .003). Body mass index increase was significantly correlated with decrease in ghrelin and adiponectin and was positively correlated with clinical improvement. This is the first study of weight gain-related hormones in children on clozapine. Hormonal changes are correlated with weight gain. How effectiveness of clozapine is linked to weight gain remains uncertain. JF - Journal of the American Academy of Child and Adolescent Psychiatry AU - Sporn, Alexandra L AU - Bobb, Aaron J AU - Gogtay, Nitin AU - Stevens, Hanna AU - Greenstein, Deanna K AU - Clasen, Liv S AU - Tossell, Julia W AU - Nugent, Thomas AU - Gochman, Peter A AU - Sharp, Wendy S AU - Mattai, Anand AU - Lenane, Marge C AU - Yanovski, Jack A AU - Rapoport, Judith L AD - Child Psychiatry Branch, NIMH, NIH, Bethesda, MD, USA. sporna@intra.nimh.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 925 EP - 933 VL - 44 IS - 9 SN - 0890-8567, 0890-8567 KW - Antipsychotic Agents KW - 0 KW - Hormones KW - Clozapine KW - J60AR2IKIC KW - Index Medicus KW - Humans KW - Hormones -- blood KW - Schizophrenic Psychology KW - Child KW - Body Mass Index KW - Adolescent KW - Male KW - Female KW - Weight Gain -- drug effects KW - Schizophrenia -- blood KW - Schizophrenia -- drug therapy KW - Clozapine -- adverse effects KW - Antipsychotic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68496232?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=HA22+%28R490A%29+Is+a+Recombinant+Immunotoxin+with+Increased+Antitumor+Activity+without+an+Increase+in+Animal+Toxicity&rft.au=Bang%2C+Sookhee%3BNagata%2C+Satoshi%3BOnda%2C+Masanori%3BKreitman%2C+Robert+J%3BPastan%2C+Ira&rft.aulast=Bang&rft.aufirst=Sookhee&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1545&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-09 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Smoky coal exposure, NBS1 polymorphisms, p53 protein accumulation, and lung cancer risk in Xuan Wei, China. AN - 68484145; 15921821 AB - Lung cancer rates in Xuan Wei County are among the highest in China and have been associated with exposure to indoor smoky coal emissions that contain high levels of polycyclic aromatic hydrocarbons (PAHs). The NBS1 gene product participates in DNA double-strand break repair and DNA damage-induced checkpoint activation, which are critical for maintaining genomic integrity. The p53 tumor suppressor gene is known to play key roles both in the maintenance of genomic stability in mammalian cells and in DNA damage surveillance. We examined the association between two common NBS1 polymorphisms (Leu34Leu, Gln185Glu) and lung cancer risk in a population-based case-control study in Xuan Wei, China. Individuals homozygous for the NBS1 34Leu or NBS1 185Glu variants were found to have an increased risk of lung cancer (odds ratio [OR] 2.15, 95% confidence interval [CI]: 0.91-5.10 and OR 2.53, 95% CI: 1.05-6.08, respectively). A haplotype containing the variant alleles from both NBS1 SNPs was associated with increased risk of lung cancer compared with the most common haplotype. Further, the associations were particularly pronounced among cases with over expression of p53 protein. These results suggest that NBS1 could be important in the pathogenesis of lung cancer in this population. However, additional studies in other populations with substantial environmental exposures to PAHs are needed to confirm our findings. JF - Lung cancer (Amsterdam, Netherlands) AU - Lan, Qing AU - Shen, Min AU - Berndt, Sonja I AU - Bonner, Matthew R AU - He, Xingzhou AU - Yeager, Meredith AU - Welch, Robert AU - Keohavong, Phouthone AU - Donahue, Mark AU - Hainaut, Pierre AU - Chanock, Stephen AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, MSC 7240, 6120 Executive Blvd., EPS 8109, Bethesda, MD 20892-7240, USA. qingl@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 317 EP - 323 VL - 49 IS - 3 SN - 0169-5002, 0169-5002 KW - Cell Cycle Proteins KW - 0 KW - Coal KW - NBN protein, human KW - Nuclear Proteins KW - Polycyclic Aromatic Hydrocarbons KW - Tumor Suppressor Protein p53 KW - Index Medicus KW - Genetic Variation KW - Odds Ratio KW - Homozygote KW - DNA Repair KW - Genome, Human KW - DNA Damage KW - Humans KW - Aged KW - Polycyclic Aromatic Hydrocarbons -- adverse effects KW - Genotype KW - Risk KW - Alleles KW - Haplotypes KW - Adult KW - Case-Control Studies KW - Environmental Exposure KW - Middle Aged KW - Male KW - Female KW - Tumor Suppressor Protein p53 -- biosynthesis KW - Lung Neoplasms -- diagnosis KW - Nuclear Proteins -- genetics KW - Coal -- adverse effects KW - Cell Cycle Proteins -- genetics KW - Polymorphism, Genetic KW - Lung Neoplasms -- genetics KW - Tumor Suppressor Protein p53 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68484145?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.atitle=Smoky+coal+exposure%2C+NBS1+polymorphisms%2C+p53+protein+accumulation%2C+and+lung+cancer+risk+in+Xuan+Wei%2C+China.&rft.au=Lan%2C+Qing%3BShen%2C+Min%3BBerndt%2C+Sonja+I%3BBonner%2C+Matthew+R%3BHe%2C+Xingzhou%3BYeager%2C+Meredith%3BWelch%2C+Robert%3BKeohavong%2C+Phouthone%3BDonahue%2C+Mark%3BHainaut%2C+Pierre%3BChanock%2C+Stephen&rft.aulast=Lan&rft.aufirst=Qing&rft.date=2005-09-01&rft.volume=49&rft.issue=3&rft.spage=317&rft.isbn=&rft.btitle=&rft.title=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.issn=01695002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-23 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inactivation of FGF8 in early mesoderm reveals an essential role in kidney development. AN - 68479696; 16049111 AB - To bypass the essential gastrulation function of Fgf8 and study its role in lineages of the primitive streak, we have used a new mouse line, T-Cre, to generate mouse embryos with pan-mesodermal loss of Fgf8 expression. Surprisingly, despite previous models in which Fgf8 has been assigned a pivotal role in segmentation/somite differentiation, Fgf8 is not required for these processes. However, mutant neonates display severe renal hypoplasia with deficient nephron formation. In mutant kidneys, aberrant cell death occurs within the metanephric mesenchyme (MM), particularly in the cortical nephrogenic zone, which provides the progenitors for recurring rounds of nephron formation. Prior to mutant morphological changes, Wnt4 and Lim1 expression, which is essential for nephrogenesis, is absent in MM. Furthermore, comparative analysis of Wnt4-null homozygotes reveals concomitant downregulation of Lim1 and diminished tubule formation. Our data support a model whereby FGF8 and WNT4 function in concert to induce the expression of Lim1 for MM survival and tubulogenesis. JF - Development (Cambridge, England) AU - Perantoni, Alan O AU - Timofeeva, Olga AU - Naillat, Florence AU - Richman, Charmaine AU - Pajni-Underwood, Sangeeta AU - Wilson, Catherine AU - Vainio, Seppo AU - Dove, Lee F AU - Lewandoski, Mark AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute, NCI-Frederick, Frederick, MD 21702, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 3859 EP - 3871 VL - 132 IS - 17 SN - 0950-1991, 0950-1991 KW - Fgf8 protein, mouse KW - 0 KW - Homeodomain Proteins KW - LIM-Homeodomain Proteins KW - Lhx1 protein, mouse KW - Proto-Oncogene Proteins KW - Transcription Factors KW - Wnt Proteins KW - Wnt4 Protein KW - Wnt4 protein, mouse KW - Fibroblast Growth Factor 8 KW - 148997-75-5 KW - Fibroblast Growth Factors KW - 62031-54-3 KW - Index Medicus KW - Animals KW - Limb Buds -- cytology KW - Limb Buds -- metabolism KW - Cell Lineage KW - Proto-Oncogene Proteins -- metabolism KW - Cell Differentiation KW - Mice KW - Limb Buds -- embryology KW - Mice, Transgenic KW - Gene Deletion KW - Alleles KW - Apoptosis -- genetics KW - Proto-Oncogene Proteins -- deficiency KW - Homeodomain Proteins -- metabolism KW - Mutation -- genetics KW - Proto-Oncogene Proteins -- genetics KW - Time Factors KW - Signal Transduction KW - Gene Expression Regulation, Developmental KW - Kidney -- metabolism KW - Kidney -- embryology KW - Kidney -- cytology KW - Mesoderm -- cytology KW - Mesoderm -- metabolism KW - Fibroblast Growth Factors -- metabolism KW - Fibroblast Growth Factors -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479696?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Development+%28Cambridge%2C+England%29&rft.atitle=Inactivation+of+FGF8+in+early+mesoderm+reveals+an+essential+role+in+kidney+development.&rft.au=Perantoni%2C+Alan+O%3BTimofeeva%2C+Olga%3BNaillat%2C+Florence%3BRichman%2C+Charmaine%3BPajni-Underwood%2C+Sangeeta%3BWilson%2C+Catherine%3BVainio%2C+Seppo%3BDove%2C+Lee+F%3BLewandoski%2C+Mark&rft.aulast=Perantoni&rft.aufirst=Alan&rft.date=2005-09-01&rft.volume=132&rft.issue=17&rft.spage=3859&rft.isbn=&rft.btitle=&rft.title=Development+%28Cambridge%2C+England%29&rft.issn=09501991&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Differential splicing of E6 within human papillomavirus type 18 variants and functional consequences. AN - 68479210; 16099904 AB - Persistent infections of the uterine cervix with 'high-risk' human papillomavirus (HPV) are now recognized as necessary for the development of cervical cancer. Among them, HPV types 16 and 18 exhibit numerous variants associated with different risks for cervical cancer development. In this study, the questions of whether different HPV type 18 variants exhibit changes in early gene transcription and the molecular mechanisms underlying these differences were investigated. It was shown that, indeed, type 18 variants exhibited singular differences in E6 transcripts in vivo. Higher levels of the E6*I transcript were detected regularly in clones harbouring the African variant, as opposed to low levels of this transcript detected in clones containing the reference clone (Asian-Amerindian), where significantly higher levels of full-length E6 transcript were usually observed. As a direct consequence, higher levels of p53 protein were found in the presence of African E6, as opposed to the low levels of p53 observed with the Asian-Amerindian E6. These variations in consequence affected the levels of cellular proteins regulated by p53, such as Bax. Similar changes in the relative levels of E6 transcripts were observed when tumours containing type 18 E6 variants were analysed. The different ability of cells containing variant E6 genes to form tumours in nude mice was suggested by the fact that tumour volumes were considerably higher when cells expressed the Asian-Amerindian E6. Mutagenesis analysis of the reference clone showed that a C491A change reverts the phenotype. These results suggest that different splicing patterns of E6 within HPV type 18 variants may possibly have biological implications in viral tumorigenesis. JF - The Journal of general virology AU - De la Cruz-Hernández, Erick AU - García-Carrancá, Alejandro AU - Mohar-Betancourt, Alejandro AU - Dueñas-González, Alfonso AU - Contreras-Paredes, Adriana AU - Pérez-Cardenas, Enrique AU - Herrera-Goepfert, Roberto AU - Lizano-Soberón, Marcela AD - Unit of Biomedical Research in Cancer, National Cancer Institute/Biomedical Research Institute, National Autonomous University of Mexico, Av. San Fernando No. 22, Col. Sección 16, Tlalpan, 14080 Mexico City, Mexico. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 2459 EP - 2468 VL - 86 SN - 0022-1317, 0022-1317 KW - DNA-Binding Proteins KW - 0 KW - E6 protein, Human papillomavirus type 18 KW - Oncogene Proteins, Viral KW - Index Medicus KW - Genetic Variation KW - Animals KW - HeLa Cells KW - Humans KW - Mice, Nude KW - Mice KW - Mice, Inbred BALB C KW - NIH 3T3 Cells KW - Female KW - Cell Line KW - Papillomavirus Infections -- physiopathology KW - Papillomaviridae -- pathogenicity KW - Papillomaviridae -- classification KW - RNA Splicing KW - Papillomavirus Infections -- virology KW - DNA-Binding Proteins -- genetics KW - Uterine Cervical Neoplasms -- physiopathology KW - Oncogene Proteins, Viral -- genetics KW - Papillomaviridae -- genetics KW - Oncogene Proteins, Viral -- metabolism KW - Uterine Cervical Neoplasms -- virology KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479210?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+general+virology&rft.atitle=Differential+splicing+of+E6+within+human+papillomavirus+type+18+variants+and+functional+consequences.&rft.au=De+la+Cruz-Hern%C3%A1ndez%2C+Erick%3BGarc%C3%ADa-Carranc%C3%A1%2C+Alejandro%3BMohar-Betancourt%2C+Alejandro%3BDue%C3%B1as-Gonz%C3%A1lez%2C+Alfonso%3BContreras-Paredes%2C+Adriana%3BP%C3%A9rez-Cardenas%2C+Enrique%3BHerrera-Goepfert%2C+Roberto%3BLizano-Sober%C3%B3n%2C+Marcela&rft.aulast=De+la+Cruz-Hern%C3%A1ndez&rft.aufirst=Erick&rft.date=2005-09-01&rft.volume=86&rft.issue=&rft.spage=2459&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+general+virology&rft.issn=00221317&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chromosomal aberrations in cell lines derived from thyroid tumors spontaneously developed in TRbetaPV/PV mice. AN - 68478155; 16102579 AB - The etiology and genetic alterations of follicular thyroid carcinoma are not well understood. By targeting a mutation (PV) into the thyroid hormone receptor beta gene (TRbetaPV mouse), we created a knock-in mutant TRbeta(PV/PV) mouse that spontaneously develop follicular thyroid carcinoma with progression to metastasis similar to human follicular thyroid carcinoma. This mouse model provides a valuable tool to ascertain the nature and the extent of genomic rearrangements that occur during carcinogenesis of the thyroid. Spectral karyotyping analysis (SKY) of seven cell lines derived from thyroid tumors developed in TRbeta(PV/PV) mice showed that all of them had abnormal karyotypes, with chromosome number ranging from near-diploid (39-42 chromosomes) to hypotetraploid (63-79 chromosomes). These seven cell lines also exhibited a variety of structural chromosomal aberrations, including common recurrent translocations and deletions. This SKY analysis shows that the development and progression of follicular thyroid carcinoma in knock-in TRbeta(PV/PV) mutant mice comprise recurrent structural and numerical genomic changes, some of which mimic those described in human thyroid cancer. JF - Cancer genetics and cytogenetics AU - Zimonjic, Drazen B AU - Kato, Yasuhito AU - Ying, Hao AU - Popescu, Nicholas C AU - Cheng, Sheue-Yann AD - Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4262, Building 37/Room 4128C, Bethesda, MD 20892-4262, USA. drazen_zimonjic@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 104 EP - 109 VL - 161 IS - 2 SN - 0165-4608, 0165-4608 KW - Thyroid Hormone Receptors beta KW - 0 KW - Thyroglobulin KW - 9010-34-8 KW - Index Medicus KW - Animals KW - Mice, Mutant Strains KW - Thyroglobulin -- analysis KW - Mice KW - Cell Line, Tumor KW - Mutation KW - Spectral Karyotyping KW - Thyroid Neoplasms -- genetics KW - Adenocarcinoma, Follicular -- metabolism KW - Thyroid Neoplasms -- metabolism KW - Adenocarcinoma, Follicular -- genetics KW - Chromosome Aberrations KW - Thyroid Hormone Receptors beta -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68478155?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+genetics+and+cytogenetics&rft.atitle=Chromosomal+aberrations+in+cell+lines+derived+from+thyroid+tumors+spontaneously+developed+in+TRbetaPV%2FPV+mice.&rft.au=Zimonjic%2C+Drazen+B%3BKato%2C+Yasuhito%3BYing%2C+Hao%3BPopescu%2C+Nicholas+C%3BCheng%2C+Sheue-Yann&rft.aulast=Zimonjic&rft.aufirst=Drazen&rft.date=2005-09-01&rft.volume=161&rft.issue=2&rft.spage=104&rft.isbn=&rft.btitle=&rft.title=Cancer+genetics+and+cytogenetics&rft.issn=01654608&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-06 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted lipidomics: signaling lipids and drugs of abuse. AN - 68476258; 16099407 JF - Prostaglandins & other lipid mediators AU - Rapaka, Rao S AU - Piomelli, Daniele AU - Spiegel, Sarah AU - Bazan, Nicolas AU - Dennis, Edward A AD - Chemistry and Physiological Systems Research Branch, Division of Basic Neuroscience and Behavioral Research, National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD 20892, USA. r82u@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 223 EP - 234 VL - 77 IS - 1-4 SN - 1098-8823, 1098-8823 KW - Pharmaceutical Preparations KW - 0 KW - Index Medicus KW - Central Nervous System -- metabolism KW - Animals KW - Pharmaceutical Preparations -- metabolism KW - Humans KW - Brain -- metabolism KW - Models, Biological KW - Substance-Related Disorders -- therapy KW - Substance-Related Disorders -- metabolism KW - Lipid Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68476258?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prostaglandins+%26+other+lipid+mediators&rft.atitle=Targeted+lipidomics%3A+signaling+lipids+and+drugs+of+abuse.&rft.au=Rapaka%2C+Rao+S%3BPiomelli%2C+Daniele%3BSpiegel%2C+Sarah%3BBazan%2C+Nicolas%3BDennis%2C+Edward+A&rft.aulast=Rapaka&rft.aufirst=Rao&rft.date=2005-09-01&rft.volume=77&rft.issue=1-4&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=Prostaglandins+%26+other+lipid+mediators&rft.issn=10988823&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-15 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):1-3 [16099385] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted lipidomics and drug abuse research. AN - 68475216; 16099406 AB - Contemporaneously with genomics and proteomics technologies, lipidomics may be recognized as the next important emerging technology. The emergence of this important area of "omics" could very well mark the beginning of "the decade of the lipids." A workshop on lipidomics was held in Washington, DC, by the National Institute on Drug Abuse (NIDA) in April 2004. The goal of the workshop was to bring together scientists working at the frontier of lipid research, to discuss their findings in this area and to promote "lipidomics," in general, but also with a special focus on its application to drug abuse research and development of therapies to treat addiction. JF - Prostaglandins & other lipid mediators AU - Rapaka, Rao S AD - Chemistry & Physiological Systems Research Branch, Division of Basic Neuroscience & Behavioral Research, National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD 20892, USA. r82u@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 219 EP - 222 VL - 77 IS - 1-4 SN - 1098-8823, 1098-8823 KW - Lipids KW - 0 KW - Index Medicus KW - Central Nervous System -- metabolism KW - Animals KW - Drug Industry -- trends KW - Humans KW - Models, Biological KW - Drug Design KW - Lipid Metabolism KW - Substance-Related Disorders -- therapy KW - Lipids -- chemistry KW - Substance-Related Disorders -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68475216?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prostaglandins+%26+other+lipid+mediators&rft.atitle=Targeted+lipidomics+and+drug+abuse+research.&rft.au=Rapaka%2C+Rao+S&rft.aulast=Rapaka&rft.aufirst=Rao&rft.date=2005-09-01&rft.volume=77&rft.issue=1-4&rft.spage=219&rft.isbn=&rft.btitle=&rft.title=Prostaglandins+%26+other+lipid+mediators&rft.issn=10988823&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-15 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):1-3 [16099385] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thermal stability of hepatitis E virus. AN - 68459855; 16088844 AB - The thermal stability of virulent hepatitis E virus (HEV) and hepatitis A virus (HAV) was compared. Fecal suspensions of virus were heated to temperatures between 45 degrees C and 70 degrees C, and residual infectivity was determined in a cell culture system that was permissive for both viruses. Although HEV was less stable than was HAV, some HEV would most likely survive the internal temperatures of rare-cooked meat. JF - The Journal of infectious diseases AU - Emerson, Suzanne U AU - Arankalle, Vidya A AU - Purcell, Robert H AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8009, USA. semerson@niaid.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 930 EP - 933 VL - 192 IS - 5 SN - 0022-1899, 0022-1899 KW - Abridged Index Medicus KW - Index Medicus KW - Microscopy, Fluorescence KW - Hepatitis E -- transmission KW - Hot Temperature KW - Hepatitis A -- virology KW - Food Microbiology KW - Humans KW - Feces -- virology KW - Hepatitis A -- transmission KW - Hepatitis E -- virology KW - Cell Line KW - Hepatitis A virus -- growth & development KW - Hepatitis E virus -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68459855?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+infectious+diseases&rft.atitle=Thermal+stability+of+hepatitis+E+virus.&rft.au=Emerson%2C+Suzanne+U%3BArankalle%2C+Vidya+A%3BPurcell%2C+Robert+H&rft.aulast=Emerson&rft.aufirst=Suzanne&rft.date=2005-09-01&rft.volume=192&rft.issue=5&rft.spage=930&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+infectious+diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-08-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effect of tumor necrosis factor antagonism on allergen-mediated asthmatic airway inflammation. AN - 68455009; 16085220 AB - To assess whether tumor necrosis factor (TNF) antagonism can attenuate eosinophilic airway inflammation in patients with mild-to-moderate allergic asthma. Randomized, double-blind, placebo-controlled trial. National Institutes of Health (NIH) Clinical Center. Twenty-six patients with mild-to-moderate allergic asthma, receiving only inhaled beta-2-agonists, who demonstrated both an early and late phase response to inhalational allergen challenge. Injection of a soluble TNF receptor (TNFR:Fc, etanercept, Enbrel) or placebo, 25mg subcutaneously, twice weekly for 2 weeks, followed by a bronchoscopic segmental allergen challenge. The primary outcome measure was whether TNFR:Fc can access the lung and inhibit TNF bioactivity. Secondary outcome measures included pulmonary eosinophilia, Th2-type cytokines, and airway hyperresponsiveness. Anti-TNF therapy was associated with transient hemiplegia in one patient, which resulted in suspension of the study. Data from the 21 participants who completed the study were analyzed. Following treatment, patients receiving anti-TNF therapy had significantly increased TNFR2 levels in epithelial lining fluid (ELF) (P<0.001), consistent with delivery of TNFR:Fc to the lung. TNF antagonism did not attenuate pulmonary eosinophilia and was associated with an increase in ELF IL-4 levels (P=0.033) at 24h following segmental allergen challenge. TNF antagonism was not associated with a change in airway hyperresponsiveness to methacholine. TNF antagonism may not be effective for preventing allergen-mediated eosinophilic airway inflammation in mild-to-moderate asthmatics. Transient hemiplegia, which may mimic an evolving stroke, may be a potential toxicity of anti-TNF therapy. JF - Respiratory medicine AU - Rouhani, Farshid N AU - Meitin, Catherine A AU - Kaler, Maryann AU - Miskinis-Hilligoss, Dianne AU - Stylianou, Mario AU - Levine, Stewart J AD - Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 6D03, MSC 1590, Bethesda, MD 20892-1590, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1175 EP - 1182 VL - 99 IS - 9 SN - 0954-6111, 0954-6111 KW - Allergens KW - 0 KW - Anti-Inflammatory Agents, Non-Steroidal KW - Cytokines KW - Immunoglobulin G KW - Receptors, Tumor Necrosis Factor KW - Tumor Necrosis Factor-alpha KW - Etanercept KW - OP401G7OJC KW - Index Medicus KW - Hemiplegia -- chemically induced KW - Anti-Inflammatory Agents, Non-Steroidal -- therapeutic use KW - Double-Blind Method KW - Cytokines -- biosynthesis KW - Humans KW - Leukocyte Count KW - Bronchial Hyperreactivity -- drug therapy KW - Airway Obstruction -- drug therapy KW - Anti-Inflammatory Agents, Non-Steroidal -- adverse effects KW - Adult KW - Middle Aged KW - Th2 Cells -- immunology KW - Bronchoalveolar Lavage Fluid -- cytology KW - Male KW - Female KW - Allergens -- immunology KW - Pulmonary Eosinophilia -- drug therapy KW - Immunoglobulin G -- adverse effects KW - Asthma -- drug therapy KW - Receptors, Tumor Necrosis Factor -- therapeutic use KW - Tumor Necrosis Factor-alpha -- antagonists & inhibitors KW - Immunoglobulin G -- therapeutic use KW - Asthma -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68455009?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Respiratory+medicine&rft.atitle=Effect+of+tumor+necrosis+factor+antagonism+on+allergen-mediated+asthmatic+airway+inflammation.&rft.au=Rouhani%2C+Farshid+N%3BMeitin%2C+Catherine+A%3BKaler%2C+Maryann%3BMiskinis-Hilligoss%2C+Dianne%3BStylianou%2C+Mario%3BLevine%2C+Stewart+J&rft.aulast=Rouhani&rft.aufirst=Farshid&rft.date=2005-09-01&rft.volume=99&rft.issue=9&rft.spage=1175&rft.isbn=&rft.btitle=&rft.title=Respiratory+medicine&rft.issn=09546111&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-07 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Localization of mesothelin in epithelial ovarian cancer. AN - 68450907; 16082249 AB - Mesothelin is a cell surface glycoprotein that is present on normal mesothelial cells and overexpressed in several cancers. On immunohistochemical examination of a limited number of ovarian tumors, increased mesothelin expression has been previously noted. The authors evaluated mesothelin expression in 48 patients with ovarian cancer who were screened for participation in phase 1 studies of a recombinant immunotoxin targeting mesothelin. Eligibility criteria for participation in the studies included mesothelin expression by more than 30% of accessible tumor cells. Sections of formalin-fixed paraffin-embedded tumor specimens were evaluated for mesothelin expression by immunohistochemistry using the anti-mesothelin monoclonal antibody K1. Between September 2000 and January 2003, 48 ovarian tumors were analyzed for mesothelin positivity. Mesothelin positivity was noted in 34 of the 48 cases evaluated (71%). These results show that mesothelin is expressed in most epithelial ovarian cancers and that mesothelin expression in ovarian cancers can be evaluated in archival material. Patients whose tumors express mesothelin could be eligible for participation in clinical trials of novel agents targeting mesothelin. JF - Applied immunohistochemistry & molecular morphology : AIMM AU - Hassan, Raffit AU - Kreitman, Robert J AU - Pastan, Ira AU - Willingham, Mark C AD - Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4264, USA. hassanr@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 243 EP - 247 VL - 13 IS - 3 SN - 1541-2016, 1541-2016 KW - Antibodies, Monoclonal KW - 0 KW - GPI-Linked Proteins KW - Immunotoxins KW - Membrane Glycoproteins KW - mesothelin KW - Index Medicus KW - Humans KW - Biopsy KW - Tissue Distribution KW - Patient Selection KW - Immunohistochemistry KW - Female KW - Antibodies, Monoclonal -- therapeutic use KW - Epithelial Cells -- pathology KW - Ovarian Neoplasms -- pathology KW - Membrane Glycoproteins -- analysis KW - Ovarian Neoplasms -- chemistry KW - Membrane Glycoproteins -- immunology KW - Ovarian Neoplasms -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68450907?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+immunohistochemistry+%26+molecular+morphology+%3A+AIMM&rft.atitle=Localization+of+mesothelin+in+epithelial+ovarian+cancer.&rft.au=Hassan%2C+Raffit%3BKreitman%2C+Robert+J%3BPastan%2C+Ira%3BWillingham%2C+Mark+C&rft.aulast=Hassan&rft.aufirst=Raffit&rft.date=2005-09-01&rft.volume=13&rft.issue=3&rft.spage=243&rft.isbn=&rft.btitle=&rft.title=Applied+immunohistochemistry+%26+molecular+morphology+%3A+AIMM&rft.issn=15412016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-10 N1 - Date created - 2005-08-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Introduction to the Special Section on Dissemination -- Dissemination Research and Research Dissemination: How can We Close the Gap? AN - 57166801; 200605137 AB - One of the greatest challenges facing health promotion & disease prevention is translating research findings into evidence-based public health & clinical practices that are actively disseminated & widely adopted. Despite the tremendous strides made in developing effective disease prevention & control programs, there has been little study of effective dissemination of evidence-based programs to & adoption by community, public health, & clinical practice settings. This special section provides a venue in which to highlight exemplary dissemination research efforts while also identifying limitations in research to date & framing important future research questions. This issue establishes a resource for investigators interested in dissemination research, with relevance to health psychology. In this sense, it can serve as a benchmark by which to examine subsequent progress. The 6 articles reflect the state of the science in dissemination research for the promotion & adoption of health behavior change interventions. 17 References. [Copyright 2005 The American Psychological Association.] JF - Health Psychology AU - Kerner, Jon AU - Rimer, Barbara AU - Emmons, Karen AD - Division Cancer Control & Population Sciences, National Cancer Instit, National Instits Health, Bethesda, MD kernerj@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 443 EP - 446 PB - American Psychological Association, Washington DC VL - 24 IS - 5 SN - 0278-6133, 0278-6133 KW - dissemination research, knowledge translation KW - Health psychology KW - Clinical practice KW - Research transfer KW - Public health KW - Dissemination KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57166801?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+Psychology&rft.atitle=Introduction+to+the+Special+Section+on+Dissemination+--+Dissemination+Research+and+Research+Dissemination%3A+How+can+We+Close+the+Gap%3F&rft.au=Kerner%2C+Jon%3BRimer%2C+Barbara%3BEmmons%2C+Karen&rft.aulast=Kerner&rft.aufirst=Jon&rft.date=2005-09-01&rft.volume=24&rft.issue=5&rft.spage=443&rft.isbn=&rft.btitle=&rft.title=Health+Psychology&rft.issn=02786133&rft_id=info:doi/10.1037%2F0278-6133.24.5.443 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-05-02 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Dissemination; Research transfer; Health psychology; Public health; Clinical practice DO - http://dx.doi.org/10.1037/0278-6133.24.5.443 ER - TY - JOUR T1 - Dental Visits among Smoking and Nonsmoking US Adults in 2000 AN - 57117950; 200602706 AB - Objective: To examine dental visits among smoking and nonsmoking adults in a nationally representative sample. Methods: Logistic regression analysis was performed, using a sample of 15,250 US adults from the Medical Expenditure Panel Survey Household Component 2000. Results: Current smokers were less likely to report dental visits (32.9%) than were nonsmokers (45.0%) during 2000. Differences were statistically significant even after accounting for other predictors of dental care use. Conclusions: Efforts to optimize the oral health of smokers and reduce serious oral diseases may benefit from addressing this lower use of dental services among smokers. 4 Tables, 23 References. Adapted from the source document. JF - American Journal of Health Behavior AU - Drilea, Susan K AU - Reid, Britt C AU - Li, Chien-Hsun AU - Hyman, Jeffrey J AU - Manski, Richard J AD - NIDCR/CDC Data Resource Center, Rockville, MD susan.drilea@ngc.com Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 462 EP - 471 VL - 29 IS - 5 SN - 1087-3244, 1087-3244 KW - dental visits, smoking, oral diseases, dental care, health education KW - Dental treatment KW - North American people KW - Dental care KW - Health education KW - Smokers KW - Nonsmokers KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57117950?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Health+Behavior&rft.atitle=Dental+Visits+among+Smoking+and+Nonsmoking+US+Adults+in+2000&rft.au=Drilea%2C+Susan+K%3BReid%2C+Britt+C%3BLi%2C+Chien-Hsun%3BHyman%2C+Jeffrey+J%3BManski%2C+Richard+J&rft.aulast=Drilea&rft.aufirst=Susan&rft.date=2005-09-01&rft.volume=48&rft.issue=3&rft.spage=839&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm049465g LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - AJHBF6 N1 - SubjectsTermNotLitGenreText - North American people; Smokers; Nonsmokers; Health education; Dental care; Dental treatment ER - TY - JOUR T1 - Response Reversal and Children with Psychopathic Tendencies: Success Is a Function of Salience of Contingency Change AN - 57092637; 200600111 AB - Background: Previous work has inconsistently reported difficulties with response reversal/extinction in children with psychopathic tendencies. Method: We tested the hypothesis that the degree of impairment seen in children with psychopathic tendencies is a function of the salience of contingency change. We investigated the performance of children with psychopathic tendencies on a novel probabilistic response reversal task involving four conditions with gradated reward-punishment contingencies (100-0, 90-10, 80-20 and 70-30; i.e., for the 100-0 contingency, responding to one object is always rewarded while responding to the other is always punished). Results: In line with predictions, the impairment seen in the children with psychopathic tendencies was an inverse function of the salience of the contingency change. Conclusions: We suggest that this data is consistent with suggestions of subtle orbital frontal cortex impairment in children with psychopathic tendencies. Illustrations, Tables, References. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Budhani, S AU - Blair, R J R AD - Unit Affective Cognitive Neuroscience, National Instit Mental Health, Bethesda, MD Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 972 EP - 981 VL - 46 IS - 9 SN - 0021-9630, 0021-9630 KW - ADHD KW - aggression KW - antisocial behavior KW - orbitofrontal cortex KW - psychopathy KW - response reversal KW - Mentally ill children KW - Psychopathy KW - Attention deficit hyperactivity disorder KW - Brain KW - Antisocial behaviour KW - Social aggression KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57092637?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Response+Reversal+and+Children+with+Psychopathic+Tendencies%3A+Success+Is+a+Function+of+Salience+of+Contingency+Change&rft.au=Budhani%2C+S%3BBlair%2C+R+J+R&rft.aulast=Budhani&rft.aufirst=S&rft.date=2005-09-01&rft.volume=46&rft.issue=9&rft.spage=972&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2004.00398.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Antisocial behaviour; Psychopathy; Mentally ill children; Attention deficit hyperactivity disorder; Social aggression; Brain DO - http://dx.doi.org/10.1111/j.1469-7610.2004.00398.x ER - TY - JOUR T1 - Hierarchical Linear Modeling Analyses of the NEO-PI-R Scales in the Baltimore Longitudinal Study of Aging AN - 57080490; 200619148 AB - The authors examined age trends in the 5 factors & 30 facets assessed by the Revised NEO Personality Inventory in Baltimore Longitudinal Study of Aging data (N = 1,944; 5,027 assessments) collected between 1989 & 2004. Consistent with cross-sectional results, hierarchical linear modeling analyses showed gradual personality changes in adulthood: a decline in Neuroticism up to age 80, stability & then decline in Extraversion, decline in Openness, increase in Agreeableness, & increase in Conscientiousness up to age 70. Some facets showed different curves from the factor they define. Birth cohort effects were modest, & there were no consistent Gender * Age interactions. Significant nonnormative changes were found for all 5 factors; they were not explained by attrition but might be due to genetic factors, disease, or life experience. Tables, Figures, Appendixes, References. [Copyright 2005 American Psychological Association.] JF - Psychology and Aging AU - Terracciano, Antonio AU - McCrae, Robert R AU - Brant, Larry J AU - Costa, Paul T, Jr AD - Gerontology Research Center, Baltimore, MD terraccianoa@grc.nia.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 493 EP - 506 PB - American Psychological Association, Washington DC VL - 20 IS - 3 SN - 0882-7974, 0882-7974 KW - five-factor model, personality change, aging, longitudinal study, HLM KW - Ageing KW - Five factor model KW - Neuroticism KW - Personality KW - Extraversion KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57080490?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychology+and+Aging&rft.atitle=Hierarchical+Linear+Modeling+Analyses+of+the+NEO-PI-R+Scales+in+the+Baltimore+Longitudinal+Study+of+Aging&rft.au=Terracciano%2C+Antonio%3BMcCrae%2C+Robert+R%3BBrant%2C+Larry+J%3BCosta%2C+Paul+T%2C+Jr&rft.aulast=Terracciano&rft.aufirst=Antonio&rft.date=2005-09-01&rft.volume=48&rft.issue=3&rft.spage=764&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm0492709 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-11-29 N1 - Last updated - 2016-09-27 N1 - CODEN - PAGIEL N1 - SubjectsTermNotLitGenreText - Personality; Ageing; Neuroticism; Extraversion; Five factor model DO - http://dx.doi.org/10.1037/0882-7974.20.3.493 ER - TY - JOUR T1 - Personality profiles of cultures: aggregate personality traits AN - 38208558; 2987636 AB - Personality profiles of cultures can be operationalized as the mean trait levels of culture members. College students from 51 cultures rated an individual from their country whom they knew well (N = 12,156). Aggregate scores on Revised NEO Personality Inventory (NEO-PI-R) scales generalized across age and sex groups, approximated the individual-level 5-factor model, and correlated with aggregate self-report personality scores and other culture-level variables. Results were not attributable to national differences in economic development or to acquiescence. Geographical differences in scale variances and mean levels were replicated, with Europeans and Americans generally scoring higher in Extraversion than Asians and Africans. Findings support the rough scalar equivalence of NEO-PI-R factors and facets across cultures and suggest that aggregate personality profiles provide insight into cultural differences. Reprinted by permission of the American Psychological Association JF - Journal of personality and social psychology AU - McCrae, Robert R AU - Terracciano, Antonio AD - National Institutes of Health Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 407 EP - 425 VL - 89 IS - 3 SN - 0022-3514, 0022-3514 KW - Sociology KW - Social psychology KW - Personality traits KW - Cross-cultural analysis KW - Regression analysis KW - Statistical analysis KW - Cultural differences KW - Empirical research KW - Aggregate analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38208558?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+personality+and+social+psychology&rft.atitle=Personality+profiles+of+cultures%3A+aggregate+personality+traits&rft.au=McCrae%2C+Robert+R%3BTerracciano%2C+Antonio&rft.aulast=McCrae&rft.aufirst=Robert&rft.date=2005-09-01&rft.volume=89&rft.issue=3&rft.spage=407&rft.isbn=&rft.btitle=&rft.title=Journal+of+personality+and+social+psychology&rft.issn=00223514&rft_id=info:doi/10.1037%2F0022-3514.89.3.407 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 11901 10404; 9429 9416 2153; 3058 971; 4200 10902; 12224 971; 10739 12228 10919; 3121 3198 3549 2688 2449 10404; 658 971 DO - http://dx.doi.org/10.1037/0022-3514.89.3.407 ER - TY - JOUR T1 - Cocaine exposure is associated with subtle compromises of infants' and mothers' social emotional behavior and dyadic features of their interaction in the face-to-face still-face paradigm AN - 38196877; 2980357 AB - Prenatal cocaine and opiate exposure are thought to subtly compromise social and emotional development. The authors observed a large sample of 236 cocaine-exposed and 459 nonexposed infants (49 were opiate exposed and 646 nonexposed) with their mothers in the face-to-face still-face paradigm. Infant and maternal behaviors were microanalytically coded. No opiate-exposure effects were detected. However, mothers of cocaine-exposed infants showed more negative engagement than other mothers. The cocaine-exposed dyads also showed higher overall levels of mismatched engagement states than other dyads, including more negative engagement when the infants were in states of neutral engagement. Infants exposed to heavier levels of cocaine showed more passive-withdrawn negative engagement and engaged in more negative affective matching with their mothers than other infants. Although effect sizes were small, cocaine exposure, especially heavy cocaine exposure, was associated with subtly negative interchanges, which may have a cumulative impact on infants' later development and their relationships with their mothers. Reprinted by permission of the American Psychological Association JF - Developmental psychology AU - Tronick, E Z AU - Messinger, D S AU - Weinberg, M K AU - Lester, B M AU - LaGasse, L AU - Seifer, R AU - Bauer, C R AU - Shankaran, S AU - Bada, H AU - Wright, L L AU - Poole, K AU - Liu, J AD - Harvard University ; University of Miami ; Brown Medical School ; Wayne State University ; University of Tennessee ; National Institute of Child Health and Human Development ; Research Triangle Institute Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 711 EP - 722 VL - 41 IS - 5 SN - 0012-1649, 0012-1649 KW - Sociology KW - Emotions KW - Opiates KW - Personal contact KW - Communication KW - Engagement KW - Child development KW - Regression analysis KW - Personality development KW - Cocaine KW - Drugs KW - Developmental psychology KW - Motherhood KW - Infants UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38196877?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+psychology&rft.atitle=Cocaine+exposure+is+associated+with+subtle+compromises+of+infants%27+and+mothers%27+social+emotional+behavior+and+dyadic+features+of+their+interaction+in+the+face-to-face+still-face+paradigm&rft.au=Tronick%2C+E+Z%3BMessinger%2C+D+S%3BWeinberg%2C+M+K%3BLester%2C+B+M%3BLaGasse%2C+L%3BSeifer%2C+R%3BBauer%2C+C+R%3BShankaran%2C+S%3BBada%2C+H%3BWright%2C+L+L%3BPoole%2C+K%3BLiu%2C+J&rft.aulast=Tronick&rft.aufirst=E&rft.date=2005-09-01&rft.volume=41&rft.issue=5&rft.spage=711&rft.isbn=&rft.btitle=&rft.title=Developmental+psychology&rft.issn=00121649&rft_id=info:doi/10.1037%2F0012-1649.41.5.711 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 3518 10404; 3755; 2197 2212 6075 3483; 9407 6823; 9421 9416 2153; 2435 3755; 8953 3755; 10739 12228 10919; 4278; 6495 2212; 8316; 4196; 2572 DO - http://dx.doi.org/10.1037/0012-1649.41.5.711 ER - TY - JOUR T1 - Mothers' and fathers' behaviors toward their 3- to 4-month-old infants in lower, middle, and upper socioeconomic African American families AN - 38196613; 2980358 AB - African American mothers' and fathers' availability, caregiving, and social behaviors toward their infants in and around their homes were examined. Twenty lower, 21 middle, and 21 upper socioeconomic families and their 3- to 4-month-old infants were observed for 4 3-hr blocks between 8:00 a.m. and 8:00 p.m. on 4 different weekdays. With increasing economic resources, children's exposure to multiple caregivers and nonresident fathers declined. Mothers were more available to infants than fathers were, regardless of socioeconomic status. Mothers fed infants more than fathers did, whereas fathers vocalized more and displayed more affection to infants than mothers did when they were examined in proportion to caregiver presence. Mothers and fathers interacted with male and female infants quite similarly, although, in the upper socioeconomic families, fathers of daughters were more available than fathers of sons. Fathers and mothers in the different socioeconomic groups held, displayed affection to, and soothed their infants differently. Reprinted by permission of the American Psychological Association JF - Developmental psychology AU - Roopnarine, Jaipaul L AU - Fouts, Hillary N AU - Lamb, Michael E AU - Lewis-Elligan, Tracey Y AD - Syracuse University ; National Institute of Child Health and Human Development ; University of Cambridge ; DePaul University Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 723 EP - 732 VL - 41 IS - 5 SN - 0012-1649, 0012-1649 KW - Sociology KW - Socioeconomic status KW - Behavioural psychology KW - Gender differentiation KW - U.S.A. KW - Child care KW - Family studies KW - Fatherhood KW - African-Americans KW - Egalitarianism KW - Developmental psychology KW - Infants KW - Motherhood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38196613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+psychology&rft.atitle=Mothers%27+and+fathers%27+behaviors+toward+their+3-+to+4-month-old+infants+in+lower%2C+middle%2C+and+upper+socioeconomic+African+American+families&rft.au=Roopnarine%2C+Jaipaul+L%3BFouts%2C+Hillary+N%3BLamb%2C+Michael+E%3BLewis-Elligan%2C+Tracey+Y&rft.aulast=Roopnarine&rft.aufirst=Jaipaul&rft.date=2005-09-01&rft.volume=41&rft.issue=5&rft.spage=723&rft.isbn=&rft.btitle=&rft.title=Developmental+psychology&rft.issn=00121649&rft_id=info:doi/10.1037%2F0012-1649.41.5.723 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 3518 10404; 5423 3549 2688 2449 10404; 4783; 8316; 4827 9182; 11988 4011 3974 9390 11932 2328 11935 5837 2360 2688 2449 10404 11936; 2192; 4112 4368 9705 9713 6203; 1540 1543 10404; 635 1656 10555 6091 961 636 4424; 6495 2212; 433 293 14 DO - http://dx.doi.org/10.1037/0012-1649.41.5.723 ER - TY - JOUR T1 - delta -Aminolevulinic Acid Dehydratase Polymorphism and Risk of Brain Tumors in Adults AN - 20715995; 6524915 AB - The enzyme delta -aminolevulinic acid dehydratase (ALAD), which catalyzes the second step of heme synthesis, can be inhibited by several chemicals, including lead, a potential risk factor for brain tumors, particularly meningioma. In this study we examined whether the ALAD G177C polymorphism in the gene coding for ALAD is associated with risk of intracranial tumors of the brain and nervous system. We use data from a case-control study with 782 incident brain tumor cases and 799 controls frequency matched on hospital, age, sex, race/ethnicity, and residential proximity to the hospital. Blood samples were drawn and DNA subsequently sent for genotyping for 73% of subjects. ALAD genotype was determined for 94% of these samples (355 glioma, 151 meningioma, 67 acoustic neuroma, and 505 controls). Having one or more copy of the ALAD2 allele was associated with increased risk for meningioma [odds ratio (OR) = 1.6; 95% confidence interval (CI), 1.0-2.6], with the association appearing stronger in males (OR = 3.5; 95% CI, 1.3-9.2) than in females (OR = 1.2; 95% CI, 0.7-2.2). No increased risk associated with the ALAD2 variant was observed for glioma or acoustic neuroma. These findings suggest that the ALAD2 allele may increase genetic susceptibility to meningioma. JF - Environmental Health Perspectives AU - Rajaraman, P AU - Schwartz, B S AU - Rothman, N AU - Yeager, M AU - Fine, HA AU - Shapiro, W R AU - Selker, R G AU - Black, P M AU - Inskip, P D AD - National Cancer Institute, NIH, DHHS, 6120 Executive Blvd., EPS Room 7085, Bethesda, MD 20892-7238, USA, rajarama@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1209 EP - 1211 VL - 113 IS - 9 SN - 0091-6765, 0091-6765 KW - Risk Abstracts; CSA Neurosciences Abstracts KW - Chemicals KW - Age KW - Heme KW - Gene polymorphism KW - tumors KW - Aminolevulinic acid KW - Genotypes KW - Lead KW - Neoplasia KW - glioma KW - Nervous system KW - Risk factors KW - Glioma KW - brain tumors KW - Races KW - Ethnic groups KW - Sex KW - Coding KW - Data processing KW - Genotyping KW - Brain KW - Enzymes KW - Brain tumors KW - DNA KW - meningioma KW - Hospitals KW - N3 11028:Neuropharmacology & toxicology KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20715995?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=delta+-Aminolevulinic+Acid+Dehydratase+Polymorphism+and+Risk+of+Brain+Tumors+in+Adults&rft.au=Rajaraman%2C+P%3BSchwartz%2C+B+S%3BRothman%2C+N%3BYeager%2C+M%3BFine%2C+HA%3BShapiro%2C+W+R%3BSelker%2C+R+G%3BBlack%2C+P+M%3BInskip%2C+P+D&rft.aulast=Rajaraman&rft.aufirst=P&rft.date=2005-09-01&rft.volume=113&rft.issue=9&rft.spage=1209&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7986 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Coding; Data processing; Heme; Genotyping; Gene polymorphism; Enzymes; Aminolevulinic acid; Neoplasia; Lead; Brain tumors; Nervous system; Risk factors; Glioma; meningioma; Ethnic groups; Races; Sex; Hospitals; Chemicals; glioma; Age; DNA; Brain; tumors; Genotypes; brain tumors DO - http://dx.doi.org/10.1289/ehp.7986 ER - TY - JOUR T1 - Reconstruction of the Conserved beta -Bulge in Mammalian Defensins Using D-Amino Acids AN - 20099445; 6527775 AB - Defensins are cationic antimicrobial mini-proteins that play important roles in the innate immune defense against microbial infection. Six invariant Cys residues in each defensin form three structurally indispensable intramolecular disulfide bridges. The only other residue invariant in all known mammalian defensins is a Gly. Structural studies indicate that the invariant Gly residue is located in an atypical, classic-type beta -bulge with the backbone torsion angles ({phi}, psi ) disallowed for L-amino acids but permissible for D-enantiomers. We replaced the invariant Gly super(17) residue in human neutrophil alpha -defensin 2 (HNP2) by L-Ala or one of the D-amino acids Ala, Glu, Phe, Arg, Thr, Val, or Tyr. Although L-Ala super(17)-HNP2 could not be folded, resulting in massive aggregation, all of the D-amino acid-substituted analogs folded with high efficiency. The high resolution x-ray crystal structures of dimeric D-Ala super(17)-HNP2 were determined in three different crystal forms, showing a well preserved beta -bulge identical to those found in other defensins. The seven D-analogs of HNP2 exhibited highly variable bactericidal activity against Gram-positive and Gram-negative test strains, consistent with the premise that interplay between charge and hydrophobicity dictates how amphiphilic defensins kill. Further, the bactericidal activity of these D-amino acid analogs of HNP2 correlated well with their ability to induce leakage from large unilamellar vesicles, supporting membrane permeabilization as the lethal event in microbial killing by HNP2. Our findings identify a conformational prerequisite in the beta -bulge of defensins essential for correct folding and native structure, thereby explaining the molecular basis of the Gly-Xaa-Cys motif conserved in all mammalian defensins. JF - Journal of Biological Chemistry AU - Xie, Cao AU - Prahl, Adam AU - Ericksen, Bryan AU - Wu, Zhibin AU - Zeng, Pengyun AU - Li, Xiangqun AU - Lu, Wei-Yue AU - Lubkowski, Jacek AU - Lu, Wuyuan AD - Institute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, Macromolecular Assembly Structure and Cell Signaling Section, NCI, National Institutes of Health, Frederick, Maryland 21702, and Fudan-PharmCo Drug Target Research Center, School of Pharmacy, Fudan University, Shanghai 200032, China Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 32921 EP - 32929 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 38 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology KW - Leakage KW - Leukocytes (neutrophilic) KW - Hydrophobicity KW - Crystals KW - Infection KW - Antimicrobial agents KW - Defensins KW - Ionizing radiation KW - Membrane vesicles KW - Crystal structure KW - D-Amino acids KW - Bactericidal activity KW - A 01340:Antibiotics & Antimicrobials KW - J 02340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20099445?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=In+the+quest+for+stable+rescuing+mutants+of+p53%3A+computational+mutagenesis+of+flexible+loop+L1.&rft.au=Pan%2C+Yongping%3BMa%2C+Buyong%3BVenkataraghavan%2C+R+Babu%3BLevine%2C+Arnold+J%3BNussinov%2C+Ruth&rft.aulast=Pan&rft.aufirst=Yongping&rft.date=2005-02-08&rft.volume=44&rft.issue=5&rft.spage=1423&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Defensins; Leakage; Ionizing radiation; Leukocytes (neutrophilic); Crystal structure; Membrane vesicles; Hydrophobicity; D-Amino acids; Crystals; Infection; Bactericidal activity; Antimicrobial agents ER - TY - JOUR T1 - A Decline in C sub(6) Antibody Titer Occurs in Successfully Treated Patients with Culture-Confirmed Early Localized or Early Disseminated Lyme Borreliosis AN - 20097863; 6529921 AB - C sub(6), a Borrelia burgdorferi-derived peptide, is used as the antigen in the C sub(6)-Lyme disease diagnostic test. We assessed retrospectively whether a fourfold decrease or a decrease to a negative value in anti-C sub(6) antibody titer is positively correlated with a positive response to treatment in a sample of culture-confirmed patients with either early localized (single erythema migrans [EM]; n = 93) or early disseminated (multiple EM; n = 27) disease. All of these patients had been treated with antibiotics and were free of disease within 6 to 12 months of follow-up. Results show that a serum specimen taken at this time was either C sub(6) negative or had a greater than or equal to 4-fold decrease in C sub(6) antibody titer with respect to a specimen taken at baseline (or during the early convalescent period if the baseline specimen was C sub(6) negative) for all of the multiple-EM patients (P < 0.0001) and in 89% of the single-EM patients (P < 0.0001). These results indicate that a decline in anti-C sub(6) antibody titer coincides with effective antimicrobial therapy in patients with early localized or early disseminated Lyme borreliosis. JF - Clinical and Diagnostic Laboratory Immunology AU - Philipp, Mario T AU - Wormser, Gary P AU - Marques, Adriana R AU - Bittker, Susan AU - Martin, Dale S AU - Nowakowski, John AU - Dally, Leonard G AD - Division of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana. Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla, New York. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland. The EMMES Corporation, Rockville, Maryland Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1069 EP - 1074 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 12 IS - 9 SN - 1071-412X, 1071-412X KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Antibodies KW - Erythema KW - Borrelia burgdorferi KW - Borreliosis KW - Borrelia KW - Antibiotics KW - Lyme disease KW - Antimicrobial agents KW - A 01340:Antibiotics & Antimicrobials KW - J 02833:Immune response and immune mechanisms KW - F 06106:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20097863?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+and+Diagnostic+Laboratory+Immunology&rft.atitle=A+Decline+in+C+sub%286%29+Antibody+Titer+Occurs+in+Successfully+Treated+Patients+with+Culture-Confirmed+Early+Localized+or+Early+Disseminated+Lyme+Borreliosis&rft.au=Philipp%2C+Mario+T%3BWormser%2C+Gary+P%3BMarques%2C+Adriana+R%3BBittker%2C+Susan%3BMartin%2C+Dale+S%3BNowakowski%2C+John%3BDally%2C+Leonard+G&rft.aulast=Philipp&rft.aufirst=Mario&rft.date=2005-09-01&rft.volume=12&rft.issue=9&rft.spage=1069&rft.isbn=&rft.btitle=&rft.title=Clinical+and+Diagnostic+Laboratory+Immunology&rft.issn=1071412X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Erythema; Antibodies; Borreliosis; Antibiotics; Antimicrobial agents; Lyme disease; Borrelia burgdorferi; Borrelia ER - TY - JOUR T1 - A Combined Proteome and Microarray Investigation of Inorganic Phosphate-induced Pre-osteoblast Cells AN - 20087560; 6530163 AB - Inorganic phosphate, which is generated during osteoblast differentiation and mineralization, has recently been identified as an important signaling molecule capable of altering signal transduction pathways and gene expression. A large scale quantitative proteomic investigation of pre-osteoblasts stimulated with inorganic phosphate for 24 h resulted in the identification of 2501 proteins, of which 410 (16%) had an altered abundance ratio of greater than or equal to 1.75-fold, either up or down, revealing both novel and previously defined osteoblast-regulated proteins. A pathway/function analysis of these proteins revealed an increase in cell cycle and proliferation that was subsequently verified by conventional biochemical means. To further analyze the mechanisms by which inorganic phosphate regulates cellular protein levels, we undertook a mRNA microarray analysis of pre-osteoblast cells at 18, 21, and 24 h after inorganic phosphate exposure. Comparison of the mRNA microarray data with the 24-hour quantitative proteomic data resulted in a generally weak overall correlation; the 21-hour RNA sample showed the highest correlation to the proteomic data. However, an analysis of osteoblast relevant proteins revealed a much higher correlation at all time points. A comparison of the microarray and proteomic datasets allowed for the identification of a number of candidate proteins that are post-transcriptionally regulated by elevated inorganic phosphate, including Fra-1, a member of the activator protein-1 family of transcription factors. The analysis of the data presented here not only sheds new light on the important roles of inorganic phosphate in osteoblast function but also begins to address the contribution of post-transcriptional and post-translational regulation to a cell's expressed proteome. The ability to accurately measure changes in both protein abundance and mRNA levels on a system-wide scale represents a novel means to extract data from previously one-dimensional datasets. JF - Molecular and Cellular Proteomics AU - Conrads, Kelly A AU - Yi, Ming AU - Simpson, Kerri A AU - Lucas, David A AU - Camalier, Corinne E AU - Yu, Li-Rong AU - Veenstra, Timothy D AU - Stephens, Robert M AU - Conrads, Thomas P AU - Beck, George RJr AD - Laboratory of Cancer Prevention, Center for Cancer Research, Advanced Biomedical Computing Center, SAIC-Frederick, Inc., and Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., National Cancer Institute, Frederick, Maryland 21702 Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1284 EP - 1296 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.asbmb.org/] VL - 4 IS - 9 SN - 1535-9476, 1535-9476 KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Osteoblastogenesis KW - Fra1 protein KW - Data processing KW - Cell cycle KW - Activator protein 1 KW - Mineralization KW - DNA microarrays KW - Gene expression KW - Osteoblasts KW - Post-translation KW - Phosphate KW - Transcription factors KW - proteomics KW - Post-transcription KW - Signal transduction KW - N 14045:Transcriptional regulation KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20087560?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+Cellular+Proteomics&rft.atitle=A+Combined+Proteome+and+Microarray+Investigation+of+Inorganic+Phosphate-induced+Pre-osteoblast+Cells&rft.au=Conrads%2C+Kelly+A%3BYi%2C+Ming%3BSimpson%2C+Kerri+A%3BLucas%2C+David+A%3BCamalier%2C+Corinne+E%3BYu%2C+Li-Rong%3BVeenstra%2C+Timothy+D%3BStephens%2C+Robert+M%3BConrads%2C+Thomas+P%3BBeck%2C+George+RJr&rft.aulast=Conrads&rft.aufirst=Kelly&rft.date=2005-09-01&rft.volume=4&rft.issue=9&rft.spage=1284&rft.isbn=&rft.btitle=&rft.title=Molecular+and+Cellular+Proteomics&rft.issn=15359476&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Osteoblastogenesis; Fra1 protein; Data processing; Activator protein 1; Cell cycle; Mineralization; DNA microarrays; Gene expression; Osteoblasts; Phosphate; Post-translation; Transcription factors; proteomics; Post-transcription; Signal transduction ER - TY - JOUR T1 - Single nucleotide polymorphism in transcriptional regulatory regions and expression of environmentally responsive genes AN - 20022293; 8252197 AB - Single nucleotide polymorphisms (SNPs) in the human genome are DNA sequence variations that can alter an individual's response to environmental exposure. SNPs in gene coding regions can lead to changes in the biological properties of the encoded protein. In contrast, SNPs in non-coding gene regulatory regions may affect gene expression levels in an allele-specific manner, and these functional polymorphisms represent an important but relatively unexplored class of genetic variation. The main challenge in analyzing these SNPs is a lack of robust computational and experimental methods. Here, we first outline mechanisms by which genetic variation can impact gene regulation, and review recent findings in this area; then, we describe a methodology for bioinformatic discovery and functional analysis of regulatory SNPs in cis-regulatory regions using the assembled human genome sequence and databases on sequence polymorphism and gene expression. Our method integrates SNP and gene databases and uses a set of computer programs that allow us to: (1) select SNPs, from among the >9 million human SNPs in the NCBI dbSNP database, that are similar to cis-regulatory element (RE) consensus sequences; (2) map the selected dbSNP entries to the human genome assembly in order to identify polymorphic REs near gene start sites; (3) prioritize the candidate polymorphic RE containing genes by searching the existing genotype and gene expression data sets. The applicability of this system has been demonstrated through studies on p53 responsive elements and is being extended to additional pathways and environmentally responsive genes. . JF - Toxicology and Applied Pharmacology AU - Wang, X AU - Tomso, D J AU - Liu, X AU - Bell, DA AD - Laboratory of Computational Biology and Risk Analysis, National Institute of Environmental Health Sciences, Mail Drop: C3-03, PO Box 12233, Research Triangle Park, NC 27709, USA, BELL1@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 84 EP - 90 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 207 IS - 2 SN - 0041-008X, 0041-008X KW - Genetics Abstracts; Toxicology Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Genomes KW - Data processing KW - Gene polymorphism KW - Nucleotide sequence KW - Regulatory sequences KW - Genetic diversity KW - Transcription KW - Computer applications KW - p53 protein KW - Computer programs KW - Databases KW - Single-nucleotide polymorphism KW - Reviews KW - Gene regulation KW - Bioinformatics KW - X 24310:Pharmaceuticals KW - N 14815:Nucleotide Sequence KW - G 07780:Fungi UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20022293?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Single+nucleotide+polymorphism+in+transcriptional+regulatory+regions+and+expression+of+environmentally+responsive+genes&rft.au=Wang%2C+X%3BTomso%2C+D+J%3BLiu%2C+X%3BBell%2C+DA&rft.aulast=Wang&rft.aufirst=X&rft.date=2005-09-01&rft.volume=207&rft.issue=2&rft.spage=84&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.09.024 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Genomes; Data processing; Regulatory sequences; Nucleotide sequence; Gene polymorphism; Transcription; Genetic diversity; Computer applications; p53 protein; Databases; Computer programs; Single-nucleotide polymorphism; Gene regulation; Reviews; Bioinformatics DO - http://dx.doi.org/10.1016/j.taap.2004.09.024 ER - TY - JOUR T1 - Serotonergic-like progenitor cells propagated from neural stem cells in vitro: survival with SERT protein expression following implantation into brains of mice lacking SERT AN - 19975698; 7327526 AB - During mammalian brain development serotonin (5-HT) contributes to neuronal proliferation, migration and differentiation in cortex and other brain regions. In adulthood the brain serotonergic system thus participates in the coordination of complex sensory and motor functions that are associated with different behavioral states and are implicated in multiple neuropsychiatric disorders. The aim of the present study was to propagate serotonergic-like progenitor cells from mouse neural stem cells, to evaluate their properties [e.g., serotonin transporter (SERT)-mediated reuptake activity and receptor-mediated electrophysiological responses], then to implant these progenitor cells into the brains of mice with a targeted disruption of SERT, to investigate whether these implanted progenitors would survive, maintain the same phenotype and express SERT in the adult brain of SERT knockout mice. JF - FASEB Journal AU - Ren-Patterson, R F AU - Kim, D-K AU - Zheng, X AU - Sherrill, S AU - Huang, S-J AU - Tolliver, T AU - Murphy, D L AD - Laboratory of Clinical Science, National Institute of Mental Health, Building 10, Room 3D41, Bethesda, MD 20892-1264, USA, renpatr@intra.nimh.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1537 EP - 1539 VL - 19 IS - 11 SN - 0892-6638, 0892-6638 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Cell survival KW - Differentiation KW - Cortex (motor) KW - Mental disorders KW - Cortex KW - Brain KW - Cell migration KW - Serotonin transporter KW - Neural stem cells KW - Serotonin KW - Cortex (somatosensory) KW - N3 11003:Developmental neuroscience KW - W 30955:Biosensors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19975698?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+Journal&rft.atitle=Serotonergic-like+progenitor+cells+propagated+from+neural+stem+cells+in+vitro%3A+survival+with+SERT+protein+expression+following+implantation+into+brains+of+mice+lacking+SERT&rft.au=Ren-Patterson%2C+R+F%3BKim%2C+D-K%3BZheng%2C+X%3BSherrill%2C+S%3BHuang%2C+S-J%3BTolliver%2C+T%3BMurphy%2C+D+L&rft.aulast=Ren-Patterson&rft.aufirst=R&rft.date=2005-09-01&rft.volume=19&rft.issue=11&rft.spage=1537&rft.isbn=&rft.btitle=&rft.title=FASEB+Journal&rft.issn=08926638&rft_id=info:doi/10.1096%2Ffj.04-3657fje LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Cell survival; Differentiation; Mental disorders; Cortex (motor); Cortex; Brain; Cell migration; Serotonin transporter; Neural stem cells; Cortex (somatosensory); Serotonin DO - http://dx.doi.org/10.1096/fj.04-3657fje ER - TY - JOUR T1 - Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray AN - 19944806; 6518231 AB - The possibility of generating neural cells from human bone-marrow-derived mesenchymal stem cells (hMSCs) by simple in vitro treatments is appealing both conceptually and practically. However, whether phenotypic modulations observed after chemical manipulation of such stem cells truly represent a genuine trans-lineage differentiation remains to be established. We have re-evaluated the effects of a frequently reported biochemical approach, based on treatment with butylated hydroxyanisole and dimethylsulphoxide, to bring about such phenotypic conversion by monitoring the morphological changes induced by the treatment in real time, by analysing the expression of phenotype-specific protein markers and by assessing the modulation of transcriptome. Video time-lapse microscopy showed that conversion of mesenchymal stem cells to a neuron-like morphology could be reproduced in normal primary fibroblasts as well as mimicked by addition of drugs eliciting cytoskeletal collapse and disruption of focal adhesion contacts. Analysis of markers revealed that mesenchymal stem cells constitutively expressed multi-lineage traits, including several pertaining to the neural one. However, the applied `neural induction' protocol neither significantly modulated the expression of such markers, nor induced de novo translation of other neural-specific proteins. Similarly, global expression profiling of over 21,000 genes demonstrated that gene transcription was poorly affected. Most strikingly, we found that the set of genes whose expression was altered by the inductive treatment did not match those sets of genes differentially expressed when comparing untreated mesenchymal stem cells and immature neural tissues. Conversely, by comparing these gene expression profiles with that obtained from comparisons between the same cells and an unrelated non-neural organ, such as liver, we found that the adopted neural induction protocol was no more effective in redirecting human mesenchymal stem cells toward a neural phenotype than toward an endodermal hepatic pathway. JF - Journal of Cell Science AU - Bertani, Nicoletta AU - Malatesta, Paolo AU - Volpi, Giorgia AU - Sonego, Paolo AU - Perris, Roberto AD - Department of Evolutionary and Functional Biology, University of Parma, Viale delle Scienze 11/a, 43100 Parma, Italy. Division for Experimental Oncology 2, The National Cancer Institute, CRO-IRCCS, Via Pedemontana Occidentale 1, Aviano 33081, Italy Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 3925 EP - 3936 PB - Company of Biologists, 140 Cowley Road Cambridge CB4 0DL UK, [URL:http://www.biologists.com/web/index.html] VL - 118 IS - 17 SN - 0021-9533, 0021-9533 KW - focal adhesion KW - man KW - Biotechnology and Bioengineering Abstracts; Genetics Abstracts; CSA Neurosciences Abstracts KW - Translation KW - Transcription KW - DNA microarrays KW - Cell adhesion KW - Fibroblasts KW - Cytoskeleton KW - Gene expression KW - Differentiation KW - Neurogenesis KW - Neuromodulation KW - Stem cells KW - Butylated hydroxyanisole KW - Microscopy KW - Dimethyl sulfoxide KW - Liver KW - Cell fate KW - Mesenchyme KW - Drugs KW - G 07433:Miscellaneous KW - N3 11070:Neurochemistry and cellular biology KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19944806?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Cell+Science&rft.atitle=Neurogenic+potential+of+human+mesenchymal+stem+cells+revisited%3A+analysis+by+immunostaining%2C+time-lapse+video+and+microarray&rft.au=Bertani%2C+Nicoletta%3BMalatesta%2C+Paolo%3BVolpi%2C+Giorgia%3BSonego%2C+Paolo%3BPerris%2C+Roberto&rft.aulast=Bertani&rft.aufirst=Nicoletta&rft.date=2005-09-01&rft.volume=118&rft.issue=17&rft.spage=3925&rft.isbn=&rft.btitle=&rft.title=Journal+of+Cell+Science&rft.issn=00219533&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-06-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Translation; Transcription; DNA microarrays; Fibroblasts; Cell adhesion; Gene expression; Cytoskeleton; Differentiation; Stem cells; Neuromodulation; Neurogenesis; Butylated hydroxyanisole; Microscopy; Liver; Dimethyl sulfoxide; Cell fate; Mesenchyme; Drugs ER - TY - JOUR T1 - Retroviral Integration Sites Correlate with Expressed Genes in Hematopoietic Stem Cells AN - 19935335; 6529896 AB - In this study, we analyzed whether retroviral integration sites in repopulating hematopoietic cells correlate with genes expressed in fractions enriched in hematopoietic stem cells (HSCs). We have previously described microarray studies of two populations enriched in HSCs: CD34 super(+)/CD38 super(-) and the slow dividing fraction of CD34 super(+)/CD38 super(-) cells (SDF). Furthermore, we demonstrated that oncoretroviral integrations in severe combined immunodeficient repopulating cells are preferentially located near the transcription start. Here, we have identified 117 corresponding cDNA clones on our micro-array representing genes with retroviral integration sites. These genes revealed a higher mean signal intensity in comparison with either all genes on the array or a subset of control genes with retroviral integrations in HeLa cells. Furthermore, these genes demonstrated a higher expression in CD34 super(+)/CD38 super(-) cells and SDF. The association of gene expression and retrovirally targeted genes observed here will help to elucidate the molecular characteristics of primitive repopulating hematopoietic cells. JF - Stem Cells AU - Wagner, Wolfgang AU - Laufs, Stephanie AU - Blake, Jonathon AU - Schwager, Christian AU - Wu, Xiaolin AU - Zeller, Jens W AU - Ho, Anthony D AU - Fruehauf, Stefan AD - Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany. German Cancer Research Center (DKFZ), Heidelberg, Germany. Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany. Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1050 EP - 1058 PB - AlphaMed Press, Inc., One Prestige Pl, Ste 290 Miamisburg OH 45342-3758 USA VL - 23 IS - 8 SN - 1066-5099, 1066-5099 KW - Genetics Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Expression vectors KW - Gene expression KW - Integration KW - Stem cells KW - Gene therapy KW - Immunodeficiency KW - Hemopoiesis KW - Transcription KW - Population studies KW - CD34 antigen KW - DNA microarrays KW - G 07720:Immunogenetics KW - W3 33181:Gene therapy vectors KW - W 30965:Miscellaneous, Reviews KW - V 22310:Genetics, Taxonomy & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19935335?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Stem+Cells&rft.atitle=Retroviral+Integration+Sites+Correlate+with+Expressed+Genes+in+Hematopoietic+Stem+Cells&rft.au=Wagner%2C+Wolfgang%3BLaufs%2C+Stephanie%3BBlake%2C+Jonathon%3BSchwager%2C+Christian%3BWu%2C+Xiaolin%3BZeller%2C+Jens+W%3BHo%2C+Anthony+D%3BFruehauf%2C+Stefan&rft.aulast=Wagner&rft.aufirst=Wolfgang&rft.date=2005-09-01&rft.volume=23&rft.issue=8&rft.spage=1050&rft.isbn=&rft.btitle=&rft.title=Stem+Cells&rft.issn=10665099&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Gene expression; Expression vectors; Integration; Stem cells; Gene therapy; Immunodeficiency; Population studies; Transcription; Hemopoiesis; CD34 antigen; DNA microarrays ER - TY - JOUR T1 - Green Tea Epigallocatechin-3-Gallate (EGCG) Modulates Amyloid Precursor Protein Cleavage and Reduces Cerebral Amyloidosis in Alzheimer Transgenic Mice AN - 19833184; 6529532 AB - Alzheimer's disease (AD) is a progressive neurodegenerative disorder pathologically characterized by deposition of beta -amyloid (A beta ) peptides as senile plaques in the brain. Recent studies suggest that green tea flavonoids may be used for the prevention and treatment of a variety of neurodegenerative diseases. Here, we report that (-)-epigallocatechin-3-gallate (EGCG), the main polyphenolic constituent of green tea, reduces A beta generation in both murine neuron-like cells (N2a) transfected with the human "Swedish" mutant amyloid precursor protein (APP) and in primary neurons derived from Swedish mutant APP-overexpressing mice (Tg APP sub(sw) line 2576). In concert with these observations, we find that EGCG markedly promotes cleavage of the alpha -C-terminal fragment of APP and elevates the N-terminal APP cleavage product, soluble APP- alpha . These cleavage events are associated with elevated alpha -secretase activity and enhanced hydrolysis of tumor necrosis factor alpha -converting enzyme, a primary candidate alpha -secretase. As a validation of these findings in vivo, we treated Tg APP sub(sw) transgenic mice overproducing A beta with EGCG and found decreased A beta levels and plaques associated with promotion of the nonamyloidogenic alpha -secretase proteolytic pathway. These data raise the possibility that EGCG dietary supplementation may provide effective prophylaxis for AD. JF - Journal of Neuroscience AU - Rezai-Zadeh, Kavon AU - Shytle, Doug AU - Sun, Nan AU - Mori, Takashi AU - Hou, Huayan AU - Jeanniton, Deborah AU - Ehrhart, Jared AU - Townsend, Kirk AU - Zeng, Jin AU - Morgan, David AU - Hardy, John AU - Town, Terrence AU - Tan, Jun AD - Neuroimmunology Laboratory, Silver Child Development Center, Department of Psychiatry and Behavioral Medicine, Center for Excellence in Aging and Brain Repair, Department of Neurosurgery, Alzheimer's Disease Research Laboratory, Department of Pharmacology, University of South Florida, Tampa, Florida 33613, Institute of Medical Science, Saitama Medical School, Saitama 350-8550, Japan, Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, and Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06519 Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 8807 EP - 8814 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 38 SN - 0270-6474, 0270-6474 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Proteolysis KW - epigallocatechin-3-gallate KW - Flavonoids KW - Data processing KW - green tea KW - Tumor necrosis factor KW - Alzheimer's disease KW - Brain KW - Enzymes KW - Transgenic mice KW - Hydrolysis KW - Amyloid precursor protein KW - Neurodegenerative diseases KW - Nervous system KW - Amyloidosis KW - Dietary supplements KW - Neurons KW - Prophylaxis KW - Secretase KW - beta -Amyloid KW - Senile plaques KW - N3 11126:Alzheimer's disease and other dementias KW - W 30935:Food Biotechnology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19833184?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Green+Tea+Epigallocatechin-3-Gallate+%28EGCG%29+Modulates+Amyloid+Precursor+Protein+Cleavage+and+Reduces+Cerebral+Amyloidosis+in+Alzheimer+Transgenic+Mice&rft.au=Rezai-Zadeh%2C+Kavon%3BShytle%2C+Doug%3BSun%2C+Nan%3BMori%2C+Takashi%3BHou%2C+Huayan%3BJeanniton%2C+Deborah%3BEhrhart%2C+Jared%3BTownsend%2C+Kirk%3BZeng%2C+Jin%3BMorgan%2C+David%3BHardy%2C+John%3BTown%2C+Terrence%3BTan%2C+Jun&rft.aulast=Rezai-Zadeh&rft.aufirst=Kavon&rft.date=2005-09-01&rft.volume=25&rft.issue=38&rft.spage=8807&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Proteolysis; epigallocatechin-3-gallate; Flavonoids; Data processing; green tea; Tumor necrosis factor; Alzheimer's disease; Brain; Enzymes; Transgenic mice; Hydrolysis; Amyloid precursor protein; Neurodegenerative diseases; Amyloidosis; Nervous system; Neurons; Dietary supplements; Prophylaxis; beta -Amyloid; Secretase; Senile plaques ER - TY - JOUR T1 - Expression and Targeting of Interleukin-4 Receptor for Primary and Advanced Ovarian Cancer Therapy AN - 19829078; 6527213 AB - Because the most characteristic property of ovarian cancer is i.p. spread, the majority of patients are diagnosed at an advanced stage, leading to limited availability of options for curative therapies. With an intent to identify targeted therapeutic approaches, we have observed that similar to 60% of 21 ovarian cancer tissue samples express a high density of interleukin-4 receptor (IL-4R), whereas normal ovarian tissues tested (n = 7) expressed no or low levels of IL-4R. To target IL-4R, we have developed IL-4 cytotoxin, in which circular-permuted IL-4 is fused to a mutated form of Pseudomonas exotoxin. This cytotoxin is specifically and highly cytotoxic to PA-1, IGROV-1, and SK-OV3 ovarian carcinoma cell lines in vitro. In addition, it shows remarkable antitumor activities against established s.c. ovarian tumors in immunodeficient animals. i.p. administration of IL-4 cytotoxin in mice with orthotopically implanted ovarian tumors caused regression of established tumors and prevented these animals from tumor metastasis. Continuous i.p. infusion of IL-4 cytotoxin prolonged survival of tumor-bearing mice even with bulky disease. These results indicate that IL-4R-targeted cytotoxin may be a useful agent for the management of patients with ovarian cancer, and further studies need to be done to evaluate its safety, tolerability, and efficacy. JF - Cancer Research AU - Kioi, Mitomu AU - Takahashi, Satoru AU - Kawakami, Mariko AU - Kawakami, Koji AU - Kreitman, Robert J AU - Puri, Raj K AD - Laboratory of Molecular Tumor Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biosystems and Cancer, Center for Cancer Research, and Laboratory of Molecular Biology, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 8388 EP - 8396 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 18 SN - 0008-5472, 0008-5472 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Interleukin 4 KW - Immunotherapy KW - Cytotoxins KW - Immunodeficiency KW - Pseudomonas KW - Tumors KW - Interleukin 4 receptors KW - Exotoxins KW - Metastases KW - Cytotoxicity KW - Tumor cell lines KW - Receptor density KW - Ovarian carcinoma KW - Antitumor activity KW - F 06152:Tumor Immunology KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19829078?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=Expression+and+Targeting+of+Interleukin-4+Receptor+for+Primary+and+Advanced+Ovarian+Cancer+Therapy&rft.au=Kioi%2C+Mitomu%3BTakahashi%2C+Satoru%3BKawakami%2C+Mariko%3BKawakami%2C+Koji%3BKreitman%2C+Robert+J%3BPuri%2C+Raj+K&rft.aulast=Kioi&rft.aufirst=Mitomu&rft.date=2005-09-01&rft.volume=65&rft.issue=18&rft.spage=8388&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-06-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Interleukin 4; Cytotoxins; Immunotherapy; Immunodeficiency; Tumors; Interleukin 4 receptors; Exotoxins; Metastases; Tumor cell lines; Cytotoxicity; Receptor density; Ovarian carcinoma; Antitumor activity; Pseudomonas ER - TY - JOUR T1 - Large-scale Expression and Purification of a G-protein-coupled Receptor for Structure Determination - An Overview AN - 19773601; 6873211 AB - Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands. JF - Journal of Structural and Functional Genomics AU - Grisshammer, Reinhard AU - White, Jim F AU - Trinh, Loc B AU - Shiloach, Joseph AD - National Institutes of Health, Bethesda, Maryland, 20892, USA, rkgriss@helix.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 159 EP - 163 PB - Springer-Verlag (Heidelberg), Tiergartenstrasse 17 Heidelberg 69121 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 6 IS - 2-3 SN - 1345-711X, 1345-711X KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Crystallization KW - Neurotensin KW - double prime G protein-coupled receptors KW - Structure-function relationships KW - Reviews KW - Escherichia coli KW - N.M.R. KW - J 02310:Genetics & Taxonomy KW - W 30945:Fermentation & Cell Culture KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19773601?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Structural+and+Functional+Genomics&rft.atitle=Large-scale+Expression+and+Purification+of+a+G-protein-coupled+Receptor+for+Structure+Determination+-+An+Overview&rft.au=Grisshammer%2C+Reinhard%3BWhite%2C+Jim+F%3BTrinh%2C+Loc+B%3BShiloach%2C+Joseph&rft.aulast=Grisshammer&rft.aufirst=Reinhard&rft.date=2005-09-01&rft.volume=6&rft.issue=2-3&rft.spage=159&rft.isbn=&rft.btitle=&rft.title=Journal+of+Structural+and+Functional+Genomics&rft.issn=1345711X&rft_id=info:doi/10.1007%2Fs10969-005-1917-6 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Crystallization; Neurotensin; Structure-function relationships; double prime G protein-coupled receptors; Reviews; N.M.R.; Escherichia coli DO - http://dx.doi.org/10.1007/s10969-005-1917-6 ER - TY - JOUR T1 - Cardiac mitochondrial compromise in 1-yr-old Erythrocebus patas monkeys perinatally-exposed to nucleoside reverse transcriptase inhibitors AN - 19537510; 8380879 AB - Hearts from 1-yr-old Erythrocebus patas monkeys were examined after in utero and 6-wk-postbirth exposure to antiretroviral nucleoside reverse transcriptase inhibitors (NRTIs). Protocols were modeled on those given to human immunodeficiency virus (HIV)-1-infected pregnant women. NRTIs were administered daily to the dams for the last 20% or 50% of gestation, and to the infants for 6 wk after birth. Exposures included: no drug (n=4); Zidovudine, 3'-azido-3'-deoxythymidine (AZT; n=4); AZT/Lamivudine, (-)- beta -L-2', 3'-Dideoxy-3'-thiacytidine (Epivir, 3TC) (n=4); AZT/Didanosine (Videx, ddI) (n=4); and Stavudine (Zerit, d4T)/3TC (n=4). Echocardiograms and clinical chemistry showed no drug-related changes, but the d4T/3TC-exposed fetuses at 6 and 12 mo had increased white cell counts (p<0.05). At 1 yr of age, oxidative phosphorylation (OXPHOS) enzyme activities were similar in heart mitochondria from all groups. Mitochondrial pathology, that included clones of damaged mitochondria (p<0.05), was found in hearts of all 1-yr drug-exposed infants. Levels of mtDNA were elevated (p<0.05) in hearts of all NRTI-exposed monkeys in the following order: control < d4T/3TC20% per 10 years in the 70s and beyond. The rate of decline for each decade was larger in men than in women from the 40s onward. Similar longitudinal rates of decline prevailed when peak {Vdot}O sub(2) was indexed per kilogram of body weight or per kilogram of fat-free mass and in all quartiles of self-reported leisure-time physical activity. When the components of peak {Vdot}O sub(2) were examined, the rate of longitudinal decline of the oxygen pulse (ie, the O sub(2) utilization per heart beat) mirrored that of peak {Vdot}O sub(2), whereas the longitudinal rate of heart rate decline averaged only 4% to 6% per 10 years, and accelerated only minimally with age. CONCLUSIONS: The longitudinal rate of decline in peak {Vdot}O sub(2) in healthy adults is not constant across the age span in healthy persons, as assumed by cross-sectional studies, but accelerates markedly with each successive age decade, especially in men, regardless of physical activity habits. The accelerated rate of decline of peak aerobic capacity has substantial implications with regard to functional independence and quality of life, not only in healthy older persons, but particularly when disease-related deficits are superimposed. JF - Circulation AU - Fleg, Jerome L AU - Morrell, Christopher H AU - Bos, Angelo G AU - Brant, Larry J AU - Talbot, Laura A AU - Wright, Jeanette G AU - Lakatta, Edward G AD - Laboratory of Cardiovascular Science (J.L.F., C.H.M., J.G.W., E.G.L.) and Laboratory of Clinical Investigation (A.G.B.) and Research Resources Branch (L.J.B.), Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Md Y1 - 2005/08/02/ PY - 2005 DA - 2005 Aug 02 SP - 674 EP - 682 PB - Lippincott Williams & Wilkins, Inc, 530 Walnut Street Philadelphia PA 19106-3621 USA, [URL:http://www.lww.com/] VL - 112 IS - 5 SN - 0009-7322, 0009-7322 KW - Physical Education Index KW - Oxygen consumption KW - Longitudinal studies KW - Measurement KW - Men KW - Women KW - Heart rate KW - Gerontology KW - Health KW - Adults KW - Exercise KW - Lifestyle KW - Strength KW - Weight KW - Gender KW - Activities KW - Aerobic capacity KW - Treadmill ergometry KW - Heart diseases KW - Sex KW - Circulatory system KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17400337?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Circulation&rft.atitle=Accelerated+Longitudinal+Decline+of+Aerobic+Capacity+in+Healthy+Older+Adults&rft.au=Fleg%2C+Jerome+L%3BMorrell%2C+Christopher+H%3BBos%2C+Angelo+G%3BBrant%2C+Larry+J%3BTalbot%2C+Laura+A%3BWright%2C+Jeanette+G%3BLakatta%2C+Edward+G&rft.aulast=Fleg&rft.aufirst=Jerome&rft.date=2005-08-02&rft.volume=112&rft.issue=5&rft.spage=674&rft.isbn=&rft.btitle=&rft.title=Circulation&rft.issn=00097322&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2006-12-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Longitudinal studies; Oxygen consumption; Measurement; Men; Heart rate; Women; Gerontology; Health; Exercise; Adults; Lifestyle; Strength; Weight; Gender; Aerobic capacity; Activities; Treadmill ergometry; Circulatory system; Sex; Heart diseases ER - TY - JOUR T1 - Parkinson's disease: a broken nosology. AN - 85390835; pmid-16092073 AB - Parkinson's disease (PD) is a clinical diagnosis. We argue here that if we are to make progress in understanding its underlying pathogenesis, there is a need to have a pathological definition of disease that includes the presence of Lewy bodies and nigral loss in the ventrolateral tier of the pars compacta of the substantia nigra. Using such a definition, there is only one certain and known cause: mutations in the alpha-synuclein gene. However, the phenotype of this one known cause is broader than PD and encompasses Lewy body dementia.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Hardy, John AU - Lees, Andrew J AD - Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA. hardyj@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - S2 EP - S4 VL - 20 Suppl 12 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Humans KW - Lewy Bodies: pathology KW - Nerve Tissue Proteins: genetics KW - Nuclear Proteins KW - Parkinson Disease: genetics KW - *Parkinson Disease: pathology KW - Parkinson Disease: physiopathology KW - Repressor Proteins KW - Substantia Nigra: pathology KW - Ubiquitin-Protein Ligases: genetics KW - alpha-Synuclein: genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85390835?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Parkinson%27s+disease%3A+a+broken+nosology.&rft.au=Hardy%2C+John%3BLees%2C+Andrew+J&rft.aulast=Hardy&rft.aufirst=John&rft.date=2005-08-01&rft.volume=20+Suppl+12&rft.issue=&rft.spage=S2&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Effects of serotonin 5-HT1A agonist in advanced Parkinson's disease. AN - 85390177; pmid-15791634 AB - Intermittent stimulation of striatal dopaminergic receptors seems to contribute to motor dysfunction in advanced Parkinson's disease (PD). With severe dopaminergic denervation, exogenous levodopa is largely decarboxylated to dopamine in serotonergic terminals. If 5-HT1A autoreceptors regulate dopamine as well as serotonin release, in parkinsonian patients inhibition of striatal serotonergic neuron firing might help maintain more physiological intrasynaptic dopamine concentrations and thus ameliorate motor fluctuations and dyskinesias. To evaluate this hypothesis, effects of a selective 5-HT1A agonist, sarizotan, given orally at 2 and 5 mg twice daily to 18 relatively advanced parkinsonian patients, were compared with baseline placebo function during a 3-week, double-blind, placebo-controlled, proof-of-concept study. Sarizotan alone or with intravenous levodopa had no effect on parkinsonian severity. But at safe and tolerable doses, sarizotan coadministration reduced levodopa-induced dyskinesias and prolonged its antiparkinsonian response (P < or = 0.05). Under the conditions of this study, our findings suggest that 5-HT1A receptor stimulation in levodopa-treated parkinsonian patients can modulate striatal dopaminergic function and that 5-HT1A agonists may be useful as levodopa adjuvants in the treatment of PD.Copyright 2005 Movement Disorder Society JF - Movement disorders : official journal of the Movement Disorder Society AU - Bara-Jimenez, William AU - Bibbiani, Francesco AU - Morris, Michael J AU - Dimitrova, Tzvetelina AU - Sherzai, Abdullah AU - Mouradian, Maral M AU - Chase, Thomas N AD - Experimental Therapeutics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA. baraw@ninds.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 932 EP - 936 VL - 20 IS - 8 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Aged KW - *Antiparkinson Agents: therapeutic use KW - Dose-Response Relationship, Drug KW - Double-Blind Method KW - Drug Evaluation: methods KW - Female KW - Humans KW - Male KW - Middle Aged KW - Organic Chemicals KW - *Parkinson Disease: drug therapy KW - *Serotonin 5-HT1 Receptor Agonists KW - Severity of Illness Index KW - Treatment Outcome UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85390177?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Effects+of+serotonin+5-HT1A+agonist+in+advanced+Parkinson%27s+disease.&rft.au=Bara-Jimenez%2C+William%3BBibbiani%2C+Francesco%3BMorris%2C+Michael+J%3BDimitrova%2C+Tzvetelina%3BSherzai%2C+Abdullah%3BMouradian%2C+Maral+M%3BChase%2C+Thomas+N&rft.aulast=Bara-Jimenez&rft.aufirst=William&rft.date=2005-08-01&rft.volume=20&rft.issue=8&rft.spage=932&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Identification of OATP1B3 as a high-affinity hepatocellular transporter of paclitaxel. AN - 70166901; 16210916 AB - Interindividual variability in paclitaxel and docetaxel pharmacokinetics, toxicity and response is extensive, and largely unexplained. We hypothesized that this is due to affinity of taxanes for an uptake transporter that indirectly regulates elimination pathways. Here, we studied accumulation of [3H]docetaxel and [3H]paclitaxel in Xenopus laevis oocytes injected with cRNA of the liver-specific organic anion transporting polypeptide (OATP) family members OATP1B1 (OATP2) or OATP1B3 (OATP8). Taxane transport by OATP1B1 expressing oocytes was not significantly different from that by water-injected controls, whereas uptake by OATP1B3 was 2.2-fold higher for docetaxel (p = 0.0007) and 3.3-fold higher for paclitaxel (p < 0.0001). OATP1B3-mediated paclitaxel transport was saturable (Michaelis-Menten constant, 6.79 microM), time-dependent, and highly sensitive to chemical inhibition. Paclitaxel uptake was not inhibited by ketoconazole or tariquidar. However, uptake was inhibited by the formulation excipient Cremophor (74.4% inhibition, p < 0.0001), cyclosporin A (25.2%, p = 0.005), glycyrrhizic acid (24.6%, p = 0.012), and hyperforin (28.4%, p = 0.003). Consistent with this finding, Cremophor was found to significantly affect the hepatic uptake of paclitaxel in mice. These data suggest that OATP1B3 is a key regulator of hepatic uptake, and may therefore play a role in the variable response to treatment with taxanes. JF - Cancer biology & therapy AU - Smith, Nicola F AU - Acharya, Milin R AU - Desai, Neil AU - Figg, William D AU - Sparreboom, Alex AD - Molecular Pharmacology Section, Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 815 EP - 818 VL - 4 IS - 8 SN - 1538-4047, 1538-4047 KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Organic Anion Transporters KW - Organic Anion Transporters, Sodium-Independent KW - Quinolines KW - SLCO1B1 protein, human KW - SLCO1B3 protein, human KW - Solute Carrier Organic Anion Transporter Family Member 1b1 KW - tariquidar KW - J58862DTVD KW - Paclitaxel KW - P88XT4IS4D KW - Ketoconazole KW - R9400W927I KW - Index Medicus KW - Animals KW - Hepatocytes -- drug effects KW - Humans KW - Mice KW - Xenopus laevis KW - Mice, Inbred Strains KW - Organic Anion Transporters -- genetics KW - Quinolines -- pharmacology KW - Oocytes -- metabolism KW - Organic Anion Transporters -- physiology KW - Ketoconazole -- pharmacology KW - Female KW - Hepatocytes -- metabolism KW - Organic Anion Transporters, Sodium-Independent -- genetics KW - Paclitaxel -- metabolism KW - Liver -- cytology KW - Organic Anion Transporters, Sodium-Independent -- antagonists & inhibitors KW - Liver -- drug effects KW - Organic Anion Transporters, Sodium-Independent -- physiology KW - Liver -- metabolism KW - Antineoplastic Agents, Phytogenic -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70166901?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+biology+%26+therapy&rft.atitle=Identification+of+OATP1B3+as+a+high-affinity+hepatocellular+transporter+of+paclitaxel.&rft.au=Smith%2C+Nicola+F%3BAcharya%2C+Milin+R%3BDesai%2C+Neil%3BFigg%2C+William+D%3BSparreboom%2C+Alex&rft.aulast=Smith&rft.aufirst=Nicola&rft.date=2005-08-01&rft.volume=4&rft.issue=8&rft.spage=815&rft.isbn=&rft.btitle=&rft.title=Cancer+biology+%26+therapy&rft.issn=15384047&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-07-05 N1 - Date created - 2006-05-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Cancer Biol Ther. 2005 Sep;4(9):1030-2 [16177563] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Elevated plasma concentrations of protease inhibitors and nonnucleoside reverse transcriptase inhibitors in patients coinfected with human immunodeficiency virus and hepatitis B or C: case series and literature review. AN - 68661687; 16207097 AB - To evaluate antiretroviral pharmacokinetics in patients who are coinfected with human immunodeficiency virus (HIV) and hepatitis B and/or C virus. Specifically, we sought to determine whether coinfection results in higher than expected concentrations of protease inhibitors and nonnucleoside reverse transcriptase inhibitors (NNRTIs). Case series. Human immunodeficiency virus clinic. Twenty-six patients infected with HIV and hepatitis B and/or C virus. Patients' plasma trough concentrations (Cmeasured) of protease inhibitors and NNRTIs were compared with population average trough concentrations reported in the literature (Cpredicted). Trough concentrations were obtained irrespective of the patients' liver function. A concentration ratio of Cmeasured: Cpredicted was determined for each patient. The mean concentration ratio of the 26 patients was 1.43 (95% confidence interval 1.08-1.78). For the six patients taking nelfinavir, the ratio of nelfinavir's active metabolite (M8): parent drug was calculated. The median M8:nelfinavir ratio for these six patients was 69% lower than what has been reported in a general HIV population. These preliminary findings suggest that trough concentrations of protease inhibitors and NNRTIs may be elevated in patients with HIV infection who are coinfected with hepatitis B and/or C, compared with a general population of patients with HIV infection. Until further investigation defines the relationship between coinfection, metabolic dysfunction, and increased antiretroviral exposure, therapeutic drug monitoring may be helpful to identify coinfected patients at risk for antiretroviral toxicity secondary to elevated plasma drug concentrations. JF - Pharmacotherapy AU - Robertson, Sarah M AU - Scarsi, Kimberly K AU - Postelnick, Michael J AU - Lynch, Patrick AD - Clinical Pharmacokinetics Research Laboratory, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. robertsonsa@cc.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1068 EP - 1072 VL - 25 IS - 8 SN - 0277-0008, 0277-0008 KW - HIV Protease Inhibitors KW - 0 KW - Reverse Transcriptase Inhibitors KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - Index Medicus KW - Liver Cirrhosis -- pathology KW - Half-Life KW - Humans KW - HIV Reverse Transcriptase -- antagonists & inhibitors KW - Retrospective Studies KW - Liver Function Tests KW - Chromatography, High Pressure Liquid KW - Hepatitis B -- complications KW - Reverse Transcriptase Inhibitors -- pharmacokinetics KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - Hepatitis B -- metabolism KW - HIV Protease Inhibitors -- blood KW - HIV Infections -- metabolism KW - Reverse Transcriptase Inhibitors -- blood KW - HIV Protease Inhibitors -- pharmacokinetics KW - HIV Protease Inhibitors -- therapeutic use KW - Reverse Transcriptase Inhibitors -- therapeutic use KW - Hepatitis C -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68661687?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacotherapy&rft.atitle=Elevated+plasma+concentrations+of+protease+inhibitors+and+nonnucleoside+reverse+transcriptase+inhibitors+in+patients+coinfected+with+human+immunodeficiency+virus+and+hepatitis+B+or+C%3A+case+series+and+literature+review.&rft.au=Robertson%2C+Sarah+M%3BScarsi%2C+Kimberly+K%3BPostelnick%2C+Michael+J%3BLynch%2C+Patrick&rft.aulast=Robertson&rft.aufirst=Sarah&rft.date=2005-08-01&rft.volume=25&rft.issue=8&rft.spage=1068&rft.isbn=&rft.btitle=&rft.title=Pharmacotherapy&rft.issn=02770008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-10-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Reversibility of experimental rabbit liver cirrhosis by portal collagenase administration. AN - 68657800; 15965490 AB - The regression of cirrhosis is associated with increased intrahepatic collagenolytic enzyme activity. We investigated whether collagenase supplementation via portal vein infusion can retard cirrhosis development and/or reverse cirrhosis. In all, 35 rabbits were initially assigned to study. However, because of high surgical mortality and infection, only 15 animals completed study. Four normal controls (group I) received olive oil subcutaneously (SC) for 12 weeks followed by normal saline portal perfusion for 12 weeks. Four (group II) received CCl(4) SC for 6 weeks followed by portal vein collagenase, 6 mg twice weekly, plus SC CCl(4) for 6 additional weeks and then killed. Four rabbits (group III) received CCl(4) SC for 12 weeks and then 6 mg of collagenase portally for 12 weeks, while three control rabbits (group IV) received CCl(4) for 12 weeks followed by saline for 12 weeks. After 12 weeks of CCl(4), liver hydroxyproline content of collagenase-treated group II (361.1+/-106.6 microg/g) was significantly reduced compared with group III+IV that had not yet received collagenase (589.0+/-162.9 microg/g; P<0.05). In the main comparison, hydroxyproline content of collagenase-treated group III (177.5+/-35.6 microg/g) was significantly decreased compared with saline-treated controls (446.3+/-150.1 microg/g; P<0.01). Further, liver histology showed complete regression of cirrhosis in the collagenase-treated animals. No toxicity of liver, kidney, lung, brain or heart was observed histologically. Anaphylaxis occurred in 2/35 original animals (one fatal). In conclusion, this study provides 'proof of principle' that collagenase portal administration can retard cirrhosis development and speed regression of established cirrhosis in the rabbit CCl(4) model. Potential application to humans is premature, but feasible, if these findings are confirmed in additional animal studies. JF - Laboratory investigation; a journal of technical methods and pathology AU - Jin, Bo AU - Alter, Harvey J AU - Zhang, Zhi-Cheng AU - Shih, J Wai-Kuo AU - Esteban, Juan M AU - Sun, Tao AU - Yang, Yun-Sheng AU - Qiu, Qi AU - Liu, Xiao-Lin AU - Yao, Lin AU - Wang, Hai-Dong AU - Cheng, Liu-Fang AD - Department of Digestive Diseases, Military Medical Graduate School, Beijing, China. bjin@cc.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 992 EP - 1002 VL - 85 IS - 8 SN - 0023-6837, 0023-6837 KW - Collagen KW - 9007-34-5 KW - Carbon Tetrachloride KW - CL2T97X0V0 KW - Collagenases KW - EC 3.4.24.- KW - Index Medicus KW - Animals KW - Infusions, Intravenous KW - Collagen -- metabolism KW - Portal Vein KW - Liver -- metabolism KW - Rabbits KW - Carbon Tetrachloride -- toxicity KW - Male KW - Liver Cirrhosis -- chemically induced KW - Liver Cirrhosis -- drug therapy KW - Liver Cirrhosis -- metabolism KW - Collagenases -- administration & dosage KW - Collagenases -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68657800?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Laboratory+investigation%3B+a+journal+of+technical+methods+and+pathology&rft.atitle=Reversibility+of+experimental+rabbit+liver+cirrhosis+by+portal+collagenase+administration.&rft.au=Jin%2C+Bo%3BAlter%2C+Harvey+J%3BZhang%2C+Zhi-Cheng%3BShih%2C+J+Wai-Kuo%3BEsteban%2C+Juan+M%3BSun%2C+Tao%3BYang%2C+Yun-Sheng%3BQiu%2C+Qi%3BLiu%2C+Xiao-Lin%3BYao%2C+Lin%3BWang%2C+Hai-Dong%3BCheng%2C+Liu-Fang&rft.aulast=Jin&rft.aufirst=Bo&rft.date=2005-08-01&rft.volume=85&rft.issue=8&rft.spage=992&rft.isbn=&rft.btitle=&rft.title=Laboratory+investigation%3B+a+journal+of+technical+methods+and+pathology&rft.issn=00236837&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-31 N1 - Date created - 2005-10-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neuropsychiatric alterations in MDMA users: preliminary findings. AN - 68611315; 16179502 AB - The use of marijuana is rampant among 3,4-methylenedioxymethamphetamine (MDMA) users. The co-occurrence of abuse of these two drugs has made it difficult to assess the specific residual effects of MDMA alone. As a first step toward identifying the effects of long-term MDMA use, we studied 8 MDMA abusers, 8 marijuana/MDMA abusers, 15 marijuana abusers (matched in marijuana use without MDMA use), and 17 control subjects. EEG, cerebral blood velocity by pulsed transcranial Doppler (TCD), and psychological measures were collected. Three-minute resting eyes-closed EEG recordings were obtained from 16 electrodes. The EEG was converted to 6 frequency bands (delta, theta, alpha-1, alpha-2, beta-1, and beta-2) using a fast Fourier transformation. Blood flow velocity was determined using a temporal window for the right and left middle cerebral arteries using TCD. Absolute log delta power in the EEG of MDMA abusers at central electrode sites was significantly higher than that of the MDMA/marijuana, marijuana abusers, and control subjects. There were also increases in alpha-2 EEG power observed only in marijuana abusers. The blood flow measure, diastolic velocity, was increased in MDMA abusers whether they used marijuana or not. Because increases in delta power and perfusion deficits are associated with some chronic disorders, our findings in these ecstasy abusers suggest that MDMA use may be associated with a drug-induced neuropathological state. More research is necessary to test these ideas. JF - Annals of the New York Academy of Sciences AU - Herning, Ronald I AU - Better, Warren AU - Tate, Kimberly AU - Cadet, Jean L AD - Molecular Neuropsychiatry Branch, National Institute on Drug Abuse, Intramural Research Program, 5500 Nathan Shock Drive, Baltimore, Maryland 21224. rherning@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 20 EP - 27 VL - 1053 SN - 0077-8923, 0077-8923 KW - Hallucinogens KW - 0 KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Index Medicus KW - Humans KW - Cerebrovascular Circulation -- drug effects KW - Electroencephalography KW - Adult KW - Ultrasonography, Doppler, Transcranial KW - Marijuana Abuse -- psychology KW - Neuropsychological Tests KW - Marijuana Abuse -- physiopathology KW - Male KW - Female KW - Psychological Tests KW - Mental Disorders -- chemically induced KW - Mental Disorders -- psychology KW - Mental Disorders -- etiology KW - Substance-Related Disorders -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68611315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Neuropsychiatric+alterations+in+MDMA+users%3A+preliminary+findings.&rft.au=Herning%2C+Ronald+I%3BBetter%2C+Warren%3BTate%2C+Kimberly%3BCadet%2C+Jean+L&rft.aulast=Herning&rft.aufirst=Ronald&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=20&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microglial NADPH oxidase mediates leucine enkephalin dopaminergic neuroprotection. AN - 68611124; 16179514 AB - Here, we report that leucine enkephalin (LE) is neuroprotective to dopaminergic (DA) neurons at femtomolar concentrations through anti-inflammatory properties. Mesencephalic neuron-glia cultures pretreated with femtomolar concentrations of LE (10(-15)-10(-13) M) protected DA neurons from lipopolysaccharide (LPS)-induced DA neurotoxicity, as determined by DA uptake assay and tyrosine hydroxylase (TH) immunocytochemistry (ICC). However, des-tyrosine leucine enkephalin (DTLE), an LE analogue that is missing the tyrosine residue required for binding to the kappa opioid receptor, was also neuroprotective (10(-15)-10(-13) M), as determined by DA uptake assay and TH ICC. Both LE and DTLE (10(-15)-10(-13) M) reduced LPS-induced superoxide production from microglia-enriched cultures. Further, both LE and DTLE (10(-14), 10(-13) M) reduced the LPS-induced tumor necrosis factor-alpha (TNFalpha) mRNA and TNFalpha protein from PHOX+/+ microglia, as determined by quantitative real-time RT-PCR and ELISA analysis in mesencephalic neuron-glia cultures, respectively. However, both peptides failed to inhibit TNFalpha expression in PHOX-/- cultures, which are unable to produce extracellular superoxide in response to LPS. Additionally, LE and DTLE (10(-14), 10(-13) M) failed to show any neuroprotection against LPS in PHOX-/- cultures. Together, these data indicate that LE and DTLE are neuroprotective at femtomolar concentrations through the inhibition of oxidative insult associated with microglial NADPH oxidase and the attenuation of the ROS-mediated amplification of TNFalpha gene expression in microglia. JF - Annals of the New York Academy of Sciences AU - Qin, Liya AU - Liu, Yuxin AU - Qian, Xun AU - Hong, Jau-Shyong AU - Block, Michelle L AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 107 EP - 120 VL - 1053 SN - 0077-8923, 0077-8923 KW - Anti-Inflammatory Agents KW - 0 KW - Indicators and Reagents KW - Lipopolysaccharides KW - Neuroprotective Agents KW - Oligopeptides KW - Tumor Necrosis Factor-alpha KW - Superoxides KW - 11062-77-4 KW - Enkephalin, Leucine KW - 58822-25-6 KW - deltakephalin KW - 85286-38-0 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Animals KW - Neurons -- drug effects KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - Pregnancy KW - Rats KW - Superoxides -- metabolism KW - Rats, Inbred F344 KW - Cells, Cultured KW - Enzyme-Linked Immunosorbent Assay KW - Lipopolysaccharides -- toxicity KW - Neurons -- enzymology KW - Oligopeptides -- pharmacology KW - Tumor Necrosis Factor-alpha -- metabolism KW - Immunohistochemistry KW - Female KW - Microglia -- enzymology KW - Dopamine -- physiology KW - NADPH Oxidase -- physiology KW - NADPH Oxidase -- genetics KW - Microglia -- drug effects KW - Enkephalin, Leucine -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68611124?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Microglial+NADPH+oxidase+mediates+leucine+enkephalin+dopaminergic+neuroprotection.&rft.au=Qin%2C+Liya%3BLiu%2C+Yuxin%3BQian%2C+Xun%3BHong%2C+Jau-Shyong%3BBlock%2C+Michelle+L&rft.aulast=Qin&rft.aufirst=Liya&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The antiapoptotic actions of mood stabilizers: molecular mechanisms and therapeutic potentials. AN - 68610580; 16179524 AB - Two primary drugs used to treat bipolar mood disorder are lithium and valproate. Emerging evidence supports the notion that both mood stabilizers have neuroprotective effects. In primary cultures of rat cerebellar granule cells and cortical neurons, lithium and valproate robustly and potently protect against glutamate-induced, N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. The neuroprotective mechanisms involve inactivation of NMDA receptors through inhibition of NR2B tyrosine phosphorylation, activation of cell survival factors such as the PI 3-kinase/Akt signaling pathway, and induction of neurotrophic/neuroprotective proteins, including brain-derived neurotrophic factor, heat-shock protein (HSP), and Bcl-2. Both drugs are also effective against other forms of insults such as ER stress in neurally related cell types. The molecular targets likely involve glycogen synthase kinase-3 (GSK-3) and histone deacetylase (HDAC) for lithium and valproate, respectively. In a rat cerebral artery occlusion model of stroke, postinsult treatment with lithium or valproate reduces ischemia-induced brain infarction, caspase-3 activation, and neurological deficits, and these neuroprotective effects are associated with HSP70 upregulation and, in the case of valproate, HDAC inhibition. In a rat excitotoxic model of Huntington's disease in which an excitotoxin is infused into the striatum to activate NMDA receptors, short-term lithium pretreatment is sufficient to protect against DNA damage, caspase activation, and apoptosis of striatal neurons, and this neuroprotection is concurrent with Bcl-2 induction. Moreover, lithium treatment increases cell proliferation near the site of striatal injury, and some newborn cells have phenotypes of neurons and astroglia. Thus, lithium and valproate are potential drugs for treating some forms of neurodegenerative diseases. JF - Annals of the New York Academy of Sciences AU - Chuang, De-Maw AD - Molecular Neurobiology Section, Biological Psychiatry Branch, Mood and Anxiety Disorders Program, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1363, USA. chuang@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 195 EP - 204 VL - 1053 SN - 0077-8923, 0077-8923 KW - Antimanic Agents KW - 0 KW - Excitatory Amino Acid Agonists KW - Valproic Acid KW - 614OI1Z5WI KW - N-Methylaspartate KW - 6384-92-5 KW - Lithium KW - 9FN79X2M3F KW - Index Medicus KW - Valproic Acid -- pharmacology KW - Rats KW - Stroke -- drug therapy KW - Animals KW - Stroke -- pathology KW - Humans KW - N-Methylaspartate -- toxicity KW - N-Methylaspartate -- antagonists & inhibitors KW - Lithium -- therapeutic use KW - Valproic Acid -- therapeutic use KW - Excitatory Amino Acid Agonists -- toxicity KW - Lithium -- pharmacology KW - Antimanic Agents -- pharmacology KW - Affect -- drug effects KW - Apoptosis -- drug effects KW - Antimanic Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68610580?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=The+antiapoptotic+actions+of+mood+stabilizers%3A+molecular+mechanisms+and+therapeutic+potentials.&rft.au=Chuang%2C+De-Maw&rft.aulast=Chuang&rft.aufirst=De-Maw&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=195&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - D2 but not D1 dopamine receptor stimulation augments brain signaling involving arachidonic acid in unanesthetized rats. AN - 68586535; 16163535 AB - Signal transduction involving the activation of phospholipase A2 (PLA2) to release arachidonic acid (AA) from membrane phospholipids, when coupled to dopamine D1- and D2-type receptors, can be imaged in rats having a chronic unilateral lesion of the substantia nigra. It is not known, however, if the signaling responses occur in the absence of a lesion. To determine this, we used our in vivo fatty acid method to measure signaling in response to D1 and D2 receptor agonists given acutely to unanesthetized rats. [1-(14)C]AA was injected intravenously in unanesthetized rats, and incorporation coefficients k* for AA (brain radioactivity/integrated plasma radioactivity) were measured using quantitative autoradiography in 61 brain regions. The animals were administered i.v. the D2 receptor agonist, quinpirole (1 mg kg(-1), i.v.), the D1 receptor agonist SKF-38393 (5 mg kg(-1), i.v.), or vehicle/saline. Quinpirole increased k* significantly in multiple brain regions rich in D2-type receptors, whereas SKF-38393 did not change k* significantly in any of the 61 regions examined. In the intact rat brain, D2 but not D1 receptors are coupled to the activation of PLA2 and the release of AA. JF - Psychopharmacology AU - Bhattacharjee, Abesh Kumar AU - Chang, Lisa AU - Lee, Ho-Joo AU - Bazinet, Richard P AU - Seemann, Ruth AU - Rapoport, Stanley I AD - Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bldg. 9, Room 1S128, Bethesda, MD 20892, USA. abeshb@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 735 EP - 742 VL - 180 IS - 4 SN - 0033-3158, 0033-3158 KW - Carbon Isotopes KW - 0 KW - Dopamine Agonists KW - Receptors, Dopamine D1 KW - Receptors, Dopamine D2 KW - Quinpirole KW - 20OP60125T KW - Arachidonic Acid KW - 27YG812J1I KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine KW - 67287-49-4 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Quinpirole -- pharmacology KW - Drug Interactions KW - Dopamine Agonists -- pharmacology KW - Carbon Isotopes -- pharmacokinetics KW - Autoradiography -- methods KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine -- pharmacology KW - Wakefulness KW - Male KW - Drug Administration Routes KW - Signal Transduction -- physiology KW - Brain -- drug effects KW - Arachidonic Acid -- pharmacokinetics KW - Signal Transduction -- drug effects KW - Brain -- physiology KW - Receptors, Dopamine D1 -- metabolism KW - Arachidonic Acid -- metabolism KW - Receptors, Dopamine D2 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68586535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=D2+but+not+D1+dopamine+receptor+stimulation+augments+brain+signaling+involving+arachidonic+acid+in+unanesthetized+rats.&rft.au=Bhattacharjee%2C+Abesh+Kumar%3BChang%2C+Lisa%3BLee%2C+Ho-Joo%3BBazinet%2C+Richard+P%3BSeemann%2C+Ruth%3BRapoport%2C+Stanley+I&rft.aulast=Bhattacharjee&rft.aufirst=Abesh&rft.date=2005-08-01&rft.volume=180&rft.issue=4&rft.spage=735&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-17 N1 - Date created - 2005-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Therapy insight: AIDS-related malignancies--the influence of antiviral therapy on pathogenesis and management. AN - 68535738; 16130937 AB - Patients with HIV infection are at an increased risk of a number of malignancies, including Kaposi's sarcoma (KS) and certain B-cell lymphomas. Most of these tumors are caused by oncogenic DNA viruses, including KS-associated herpesvirus and Epstein-Barr virus. HIV contributes to the development of these tumors through several mechanisms, including immunodeficiency, immunodysregulation, and the effects of HIV proteins such as Tat. The development of highly active antiretroviral therapy (HAART) has reduced the incidence of many HIV-associated tumors and has generally improved their responsiveness to therapy. However, the number of people living with AIDS is increasing, and it is possible that the number of AIDS-associated malignancies will rise and the pattern of tumors will change as more people live longer with HIV infection. The goal of KS therapy is long-term tumor control with minimal toxicity. HAART is an important component of this therapy, and some patients do not require other KS-specific therapies. By contrast, the goal of AIDS-related lymphoma therapy in most cases is the attainment of a complete response with curative intent, and the benefits of administering HAART during therapy must be weighed against possible disadvantages. The past decade has seen substantial improvements in the treatment of AIDS-related lymphoma, which is attributed partially to a shift in tumor type and more effective regimens. There is currently an interest in developing new therapies for HIV-associated malignancies, based on viral, vascular or other pathogenesis-based targets. JF - Nature clinical practice. Oncology AU - Yarchoan, Robert AU - Tosato, Giovanna AU - Little, Richard F AD - HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1868, USA. yarchoan@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 406 EP - 15; quiz 423 VL - 2 IS - 8 SN - 1743-4254, 1743-4254 KW - Antineoplastic Agents KW - 0 KW - Index Medicus KW - Sarcoma, Kaposi -- drug therapy KW - Sarcoma, Kaposi -- physiopathology KW - Humans KW - Lymphoma, AIDS-Related -- physiopathology KW - Lymphoma, AIDS-Related -- drug therapy KW - Sarcoma, Kaposi -- virology KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Neoplasms -- drug therapy KW - Neoplasms -- virology KW - HIV Infections -- complications KW - Antineoplastic Agents -- administration & dosage KW - Neoplasms -- physiopathology KW - Antiretroviral Therapy, Highly Active KW - Antineoplastic Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68535738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+clinical+practice.+Oncology&rft.atitle=Therapy+insight%3A+AIDS-related+malignancies--the+influence+of+antiviral+therapy+on+pathogenesis+and+management.&rft.au=Yarchoan%2C+Robert%3BTosato%2C+Giovanna%3BLittle%2C+Richard+F&rft.aulast=Yarchoan&rft.aufirst=Robert&rft.date=2005-08-01&rft.volume=2&rft.issue=8&rft.spage=406&rft.isbn=&rft.btitle=&rft.title=Nature+clinical+practice.+Oncology&rft.issn=17434254&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-08-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Agricultural antibiotics and human health. AN - 68517902; 15984910 JF - PLoS medicine AU - Smith, David L AU - Dushoff, Jonathan AU - Morris, J Glenn AD - Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA. smitdave@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1 VL - 2 IS - 8 KW - Anti-Bacterial Agents KW - 0 KW - Food Additives KW - Glycopeptides KW - Vancomycin KW - 6Q205EH1VU KW - avoparcin KW - WJ13O9MNTI KW - Index Medicus KW - Animals KW - Vancomycin -- adverse effects KW - Animal Feed KW - Humans KW - Enterococcus -- drug effects KW - Glycopeptides -- adverse effects KW - Animals, Domestic -- growth & development KW - Vancomycin Resistance KW - Animals, Domestic -- microbiology KW - Community-Acquired Infections -- transmission KW - Agriculture -- methods KW - Public Health KW - Food Additives -- adverse effects KW - Anti-Bacterial Agents -- adverse effects KW - Community-Acquired Infections -- microbiology KW - Zoonoses -- microbiology KW - Disease Transmission, Infectious KW - Zoonoses -- transmission UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68517902?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=PLoS+medicine&rft.atitle=Agricultural+antibiotics+and+human+health.&rft.au=Smith%2C+David+L%3BDushoff%2C+Jonathan%3BMorris%2C+J+Glenn&rft.aulast=Smith&rft.aufirst=David&rft.date=2005-08-01&rft.volume=2&rft.issue=8&rft.spage=e232&rft.isbn=&rft.btitle=&rft.title=PLoS+medicine&rft.issn=1549-1676&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-08-18 N1 - Date created - 2005-08-25 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Acta Vet Scand Suppl. 1999;92:51-7 [10783717] Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):3153-8 [15677330] J Antimicrob Chemother. 2000 May;45(5):677-80 [10797092] J Infect Dis. 2000 Sep;182(3):816-23 [10950776] Clin Microbiol Rev. 2000 Oct;13(4):686-707 [11023964] Eur J Clin Microbiol Infect Dis. 2000 Nov;19(11):816-22 [11152305] Appl Environ Microbiol. 2001 Apr;67(4):1494-502 [11282596] Emerg Infect Dis. 2001 Mar-Apr;7(2):183-7 [11294702] Nature. 2001 Jan 4;409(6816):37-8 [11343104] Antimicrob Agents Chemother. 2001 Jul;45(7):2054-9 [11408222] Vet Res. 2001 May-Aug;32(3-4):311-21 [11432422] Philos Trans R Soc Lond B Biol Sci. 2001 Jul 29;356(1411):983-9 [11516376] N Engl J Med. 2001 Oct 18;345(16):1161-6 [11642232] Clin Infect Dis. 2001 Nov 15;33(10):1739-46 [11595995] Clin Microbiol Infect. 2001;7 Suppl 4:16-33 [11688531] Lancet Infect Dis. 2001 Dec;1(5):314-25 [11871804] Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6434-9 [11972035] Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5752-4 [11983874] Int J Food Microbiol. 2002 Jun 5;76(1-2):143-50 [12038571] Antimicrob Agents Chemother. 2002 Sep;46(9):2779-83 [12183228] Lancet Infect Dis. 2003 Apr;3(4):241-9 [12679267] Int J Food Microbiol. 2003 Aug 1;84(3):273-84 [12810291] Int J Food Microbiol. 2003 Aug 25;85(3):213-26 [12878380] APMIS. 2003 Jun;111(6):669-72 [12969023] Emerg Infect Dis. 2003 Sep;9(9):1108-15 [14519248] Environ Health Perspect. 2003 Oct;111(13):1590-4 [14527837] Antimicrob Agents Chemother. 2003 Dec;47(12):3825-30 [14638490] J Antimicrob Chemother. 2004 Jan;53(1):28-52 [14657094] Environ Microbiol. 2004 Jan;6(1):55-9 [14686941] Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3709-14 [14985511] Clin Infect Dis. 2004 Apr 15;38(8):1108-15 [15095215] J Antimicrob Chemother. 2004 Jul;54(1):274-5; author reply 276-8 [15140862] Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10223-8 [15220470] J Antimicrob Chemother. 2004 Aug;54(2):311-20 [15215223] Int J Antimicrob Agents. 2004 Sep;24(3):205-12 [15325422] Ann Intern Med. 1995 Aug 15;123(4):250-9 [7611590] J Clin Microbiol. 1995 Nov;33(11):2842-6 [8576330] J Infect Dis. 1996 May;173(5):1129-36 [8627064] Lancet. 1997 Apr 26;349(9060):1258 [9130981] Lancet. 1997 Jul 12;350(9071):146-7 [9228991] J Antimicrob Chemother. 1997 Sep;40(3):454-6 [9338505] J Clin Microbiol. 1997 Dec;35(12):3026-31 [9399488] J Infect Dis. 1998 Feb;177(2):378-82 [9466524] Antimicrob Agents Chemother. 1998 May;42(5):1303-4 [9593175] J Clin Microbiol. 1998 Jul;36(7):1927-32 [9650938] Infect Control Hosp Epidemiol. 1998 Aug;19(8):539-45 [9758052] Emerg Infect Dis. 1999 May-Jun;5(3):329-35 [10341169] Antimicrob Agents Chemother. 1999 Sep;43(9):2215-21 [10471567] Nature. 1999 Sep 16;401(6750):233-4 [10499578] Comment In: PLoS Med. 2005 Nov;2(11):e383 [16288558] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Herbs and cytotoxic drugs: recognizing and communicating potentially relevant interactions. AN - 68508918; 16117219 JF - Clinical journal of oncology nursing AU - Lee, Colleen O AD - Office of Complementary and Alternative Medicine, National Cancer Institute, Bethesda, MD, USA. leeco@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 481 EP - 487 VL - 9 IS - 4 SN - 1092-1095, 1092-1095 KW - Antineoplastic Agents KW - 0 KW - Nursing KW - Nursing Assessment KW - Humans KW - Neoplasms -- drug therapy KW - Antineoplastic Agents -- administration & dosage KW - Herb-Drug Interactions KW - Antineoplastic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68508918?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+journal+of+oncology+nursing&rft.atitle=Herbs+and+cytotoxic+drugs%3A+recognizing+and+communicating+potentially+relevant+interactions.&rft.au=Lee%2C+Colleen+O&rft.aulast=Lee&rft.aufirst=Colleen&rft.date=2005-08-01&rft.volume=9&rft.issue=4&rft.spage=481&rft.isbn=&rft.btitle=&rft.title=Clinical+journal+of+oncology+nursing&rft.issn=10921095&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-08-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dopamine-dependent and dopamine-independent actions of cocaine as revealed by brain thermorecording in freely moving rats. AN - 68504823; 16115216 AB - Brain temperature fluctuates biphasically in response to repeated, intravenous (i.v.) cocaine injections, perhaps reflecting cocaine's inhibiting effect on both dopamine (DA) transporters and Na+ channels. By using a DA receptor blockade, one could separate these actions and determine the role of DA-dependent and DA-independent mechanisms in mediating this temperature fluctuation. Rats were chronically implanted with thermocouple probes in the brain, a non-locomotor head muscle and subcutaneously. Temperature fluctuations associated with ten repeated i.v. cocaine injections (1 mg/kg with 8-min inter-injection intervals) were examined after a combined, systemic administration of selective D1-like and D2-like receptor blockers (SCH-23390 and eticlopride) at doses that effectively inhibit DA transmission. In contrast to the initial temperature increases and subsequent biphasic fluctuations (decreases followed by increases) seen with repeated cocaine injections in saline-treated control, brain and muscle temperatures during DA receptor blockade decreased with each repeated cocaine injection. DA receptor blockade had no effects on skin temperature, which tonically decreased and biphasically fluctuated (decreases followed by increases) during repeated cocaine injections in both conditions. DA receptor blockade by itself slightly increased brain and muscle temperatures, with no evident effect on skin temperature. DA antagonists also strongly decreased spontaneous movement activity and completely blocked the locomotor activation normally induced by repeated cocaine injections. Although our data confirm that cocaine's inhibitory action on presynaptic DA uptake is essential for its ability to induce metabolic and behavioral activation, they also suggest that the physiological effects of this drug cannot be explained through this system alone. The continued hypothermic effect of cocaine points to its action on other central systems (particularly blockade of Na+ channels) that may be important for the development of cocaine abuse and adverse effects of this drug. JF - The European journal of neuroscience AU - Kiyatkin, Eugene A AU - Brown, P Leon AD - Cellular Neurobiology Branch, National Institute on Drug Abuse-Intramural Research Program, National Institutes of Health, DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. ekiyatki@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 930 EP - 938 VL - 22 IS - 4 SN - 0953-816X, 0953-816X KW - Benzazepines KW - 0 KW - Dopamine Antagonists KW - Dopamine Uptake Inhibitors KW - Salicylamides KW - Cocaine KW - I5Y540LHVR KW - eticlopride KW - J8M468HBH4 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats KW - Animals KW - Drug Interactions KW - Benzazepines -- pharmacology KW - Rats, Long-Evans KW - Dopamine Antagonists -- pharmacology KW - Salicylamides -- pharmacology KW - Motor Activity -- drug effects KW - Time Factors KW - Dopamine -- pharmacology KW - Body Temperature -- drug effects KW - Nucleus Accumbens -- drug effects KW - Nucleus Accumbens -- physiology KW - Wakefulness -- physiology KW - Body Temperature -- physiology KW - Cocaine -- pharmacology KW - Dopamine Uptake Inhibitors -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68504823?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+European+journal+of+neuroscience&rft.atitle=Dopamine-dependent+and+dopamine-independent+actions+of+cocaine+as+revealed+by+brain+thermorecording+in+freely+moving+rats.&rft.au=Kiyatkin%2C+Eugene+A%3BBrown%2C+P+Leon&rft.aulast=Kiyatkin&rft.aufirst=Eugene&rft.date=2005-08-01&rft.volume=22&rft.issue=4&rft.spage=930&rft.isbn=&rft.btitle=&rft.title=The+European+journal+of+neuroscience&rft.issn=0953816X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-18 N1 - Date created - 2005-08-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inflammation and cancer: when NF-kappaB amalgamates the perilous partnership. AN - 68479663; 16101381 AB - Chronic inflammation has long been suggested to constitute a risk factor for a variety of epithelial cancers such as malignancies of prostate, cervix, esophagus, stomach, liver, colon, pancreas, and bladder. An inflammatory response is typically accompanied by generation of free radicals, stimulation of cytokines, chemokines, growth and angiogenic factors. Free radicals, capable of both directly damaging DNA and affecting the DNA repair machinery, enhance genetic instability of affected cells, thus contributing to the first stage of neoplastic transformation also known as "initiation". Cytokines and growth factors can further promote tumor growth by stimulating cell proliferation, adhesion, vascularization, and metastatic potential of later stage tumors. Nuclear factor kappa B (NF-kappaB) is a family of ubiquitously expressed transcription factors that are widely believed to trigger both the onset and the resolution of inflammation. NF-kappaB also governs the expression of genes encoding proteins essential in control of stress response, maintenance of intercellular communications, and regulation of cellular proliferation and apoptosis. Recent data have expanded the concept of inflammation as a critical component in carcinogenesis suggesting new anti-inflammatory therapies for a complementary approach in treating a variety of tumor types. These observations highlighted the NF-kappaB pathway as an attractive avenue for drug discovery and development. The present review will outline recent advances in our understanding of NF-kappaB function in the inflammatory processes and its input in tumor initiation/promotion, as well as summarize the development of animal and cell culture models for validating drug candidates with NF-kappaB-modulating activities, and applications of the latter in cancer therapy. JF - Current cancer drug targets AU - Dobrovolskaia, Marina A AU - Kozlov, Serguei V AD - National Cancer Institute at Frederick, Boyles St., P.O. Box B, Frederick, MD 21702, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 325 EP - 344 VL - 5 IS - 5 SN - 1568-0096, 1568-0096 KW - Antineoplastic Agents KW - 0 KW - Cytokines KW - Free Radicals KW - Membrane Glycoproteins KW - NF-kappa B KW - Receptors, Cell Surface KW - Receptors, Tumor Necrosis Factor, Type II KW - Toll-Like Receptors KW - Tumor Necrosis Factor-alpha KW - Index Medicus KW - Receptors, Cell Surface -- metabolism KW - Animals KW - Humans KW - Signal Transduction -- drug effects KW - Gene Expression Regulation KW - Cytokines -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Free Radicals -- metabolism KW - Drug Design KW - Receptors, Tumor Necrosis Factor, Type II -- metabolism KW - Membrane Glycoproteins -- metabolism KW - Neoplasms -- drug therapy KW - Inflammation -- physiopathology KW - Neoplasms -- pathology KW - Inflammation -- etiology KW - Inflammation -- metabolism KW - Antineoplastic Agents -- therapeutic use KW - Antineoplastic Agents -- pharmacology KW - Neoplasms -- etiology KW - NF-kappa B -- genetics KW - NF-kappa B -- metabolism KW - NF-kappa B -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479663?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+cancer+drug+targets&rft.atitle=Inflammation+and+cancer%3A+when+NF-kappaB+amalgamates+the+perilous+partnership.&rft.au=Dobrovolskaia%2C+Marina+A%3BKozlov%2C+Serguei+V&rft.aulast=Dobrovolskaia&rft.aufirst=Marina&rft.date=2005-08-01&rft.volume=5&rft.issue=5&rft.spage=325&rft.isbn=&rft.btitle=&rft.title=Current+cancer+drug+targets&rft.issn=15680096&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - First- and second-generation antipsychotic medication and cognitive processing in schizophrenia. AN - 68478870; 16098285 AB - Schizophrenia has been consistently characterized by deficits in the cognitive domains of executive function, working memory, attention, and episodic memory. Although some cognitive abnormalities, such as motor slowing, may be associated with antipsychotic medication administration, generally the cognitive deficits shown by patients with schizophrenia can be attributed at least in part to the disease process. Modulation of the dopamine neurotransmitter system, notably through D2 receptor blockade, has been associated with psychotic symptom reduction and cognitive performance improvements in patients with schizophrenia. Although first-generation antipsychotic medication treatment initially was thought not to result in cognitive improvement, recent studies comparing second-generation antipsychotics to low doses of first-generation antipsychotic medication showed cognitive benefits for first-generation drugs, although perhaps not as great as that found after treatment with second-generation medication. Cognitive improvement associated with administration of antipsychotic medication may be a manifestation of improvement in general cortical information processing. Recent work has shown that specific genetic polymorphisms may interact with antipsychotic medication treatment to influence the degree to which cognitive abilities display improvement after treatment. In particular, the catechol-O-methyltransferase val108/158met polymorphism has been shown to predict working memory improvement after administration of antipsychotic medication to patients with schizophrenia. JF - Current psychiatry reports AU - Weickert, Thomas W AU - Goldberg, Terry E AD - Clinical Brain Disorders Branch, Genes, Cognition, and Psychosis Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 304 EP - 310 VL - 7 IS - 4 SN - 1523-3812, 1523-3812 KW - Antipsychotic Agents KW - 0 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Genotype KW - Psychomotor Performance -- drug effects KW - Polymorphism, Genetic -- genetics KW - Humans KW - Dopamine -- metabolism KW - Reaction Time -- drug effects KW - Cognition -- drug effects KW - Antipsychotic Agents -- therapeutic use KW - Antipsychotic Agents -- pharmacology KW - Cognition Disorders -- genetics KW - Schizophrenia -- drug therapy KW - Schizophrenia -- genetics KW - Antipsychotic Agents -- adverse effects KW - Schizophrenia -- complications KW - Cognition Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68478870?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+psychiatry+reports&rft.atitle=First-+and+second-generation+antipsychotic+medication+and+cognitive+processing+in+schizophrenia.&rft.au=Weickert%2C+Thomas+W%3BGoldberg%2C+Terry+E&rft.aulast=Weickert&rft.aufirst=Thomas&rft.date=2005-08-01&rft.volume=7&rft.issue=4&rft.spage=304&rft.isbn=&rft.btitle=&rft.title=Current+psychiatry+reports&rft.issn=15233812&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-12 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Bladder cancer screening and monitoring of 4,4'-methylenebis(2-chloroaniline) exposure among workers in Taiwan. AN - 68475838; 16098360 AB - Transitional cell carcinoma of the urinary bladder is associated with occupational exposure to 4,4'-methylenebis(2-chloroaniline) (MBOCA). A program to monitor MBOCA levels in the work environment and to screen for bladder cancer was performed at four MBOCA manufacturing factories. The U.S. Occupational Safety and Health Administration analytic method No. 24 was adopted in this study to measure air MBOCA concentrations. A total of 70 MBOCA-exposed workers and another 92 nonexposed workers were recruited for screening. Urine occult blood tests, urine cytology, tests for the urine tumor marker nuclear matrix protein, and abdominal ultrasonography were performed in all patients. Intravenous urography and cystoscopy were used to confirm the presence of bladder cancer. The air concentration of MBOCA was greatest in the purification area (0.23 to 0.41 mg/m3), followed by the washing area (less than 0.02 to 0.08 mg/m3) and neutralization area (less than 0.05 to 0.06 mg/m3). This study identified a current worker with proved bladder cancer. In addition, we also identified 1 worker with suspected malignant cells on urine cytology and 1 worker with atypical cytology combined with gross hematuria. Although the prevalence of atypical urinary cells and the nuclear matrix protein 22 tumor marker was not significantly different between the MBOCA-exposed workers and nonexposed workers as a whole or when grouped by sex, the prevalence of positive occult blood was marginally significantly (P = 0.055) greater in male exposed workers (18%) than in male nonexposed workers (7%). The findings of this study support the conclusions from other studies that MBOCA is potentially carcinogenic to humans. Control measures are needed to prevent overexposure from inhalation and skin absorption. JF - Urology AU - Chen, Hong-I AU - Liou, Saou-Hsing AU - Loh, Ching-Hui AU - Uang, Shi-Nian AU - Yu, Yi-Chun AU - Shih, Tung-Sheng AD - Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Nei-Hu, Taipei, Taiwan, Republic of China. hong_i@ndmctsgh.edu.tw Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 305 EP - 310 VL - 66 IS - 2 KW - Methylenebis(chloroaniline) KW - 3L2W5VTT2A KW - Index Medicus KW - Mass Screening KW - Taiwan KW - Humans KW - Adult KW - Male KW - Female KW - Occupational Exposure KW - Occupational Diseases -- diagnosis KW - Urinary Bladder Neoplasms -- diagnosis KW - Carcinoma, Transitional Cell -- diagnosis KW - Carcinoma, Transitional Cell -- chemically induced KW - Occupational Diseases -- chemically induced KW - Urinary Bladder Neoplasms -- chemically induced KW - Methylenebis(chloroaniline) -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68475838?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Urology&rft.atitle=Bladder+cancer+screening+and+monitoring+of+4%2C4%27-methylenebis%282-chloroaniline%29+exposure+among+workers+in+Taiwan.&rft.au=Chen%2C+Hong-I%3BLiou%2C+Saou-Hsing%3BLoh%2C+Ching-Hui%3BUang%2C+Shi-Nian%3BYu%2C+Yi-Chun%3BShih%2C+Tung-Sheng&rft.aulast=Chen&rft.aufirst=Hong-I&rft.date=2005-08-01&rft.volume=66&rft.issue=2&rft.spage=305&rft.isbn=&rft.btitle=&rft.title=Urology&rft.issn=1527-9995&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-28 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Diagnosis and management of primary intraocular lymphoma. AN - 68461233; 16083834 AB - Primary intraocular lymphoma (PIOL) is a subset of primary central nervous system lymphoma. The incidence of PIOL has increased in the past 20 years. PIOL often presents as chronic uveitis that is resistant to corticosteroid therapy. Diagnosing PIOL can be challenging and requires an expert pathologist. The treatment of PIOL is difficult because of its high recurrence rate and refractory nature. The objective for the future is to improve diagnostic techniques and therapeutic success while minimizing ocular toxicities. JF - Hematology/oncology clinics of North America AU - Levy-Clarke, Grace A AU - Chan, Chi-Chao AU - Nussenblatt, Robert B AD - Laboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 10N112, Bethesda, MD 20892, USA. clarkeg@nei.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 739 EP - 49, viii VL - 19 IS - 4 SN - 0889-8588, 0889-8588 KW - Index Medicus KW - Humans KW - Lymphoma -- therapy KW - Eye Neoplasms -- therapy KW - Eye Neoplasms -- diagnosis KW - Lymphoma -- diagnosis KW - Central Nervous System Neoplasms -- therapy KW - Lymphoma -- genetics KW - Central Nervous System Neoplasms -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68461233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Hematology%2Foncology+clinics+of+North+America&rft.atitle=Diagnosis+and+management+of+primary+intraocular+lymphoma.&rft.au=Levy-Clarke%2C+Grace+A%3BChan%2C+Chi-Chao%3BNussenblatt%2C+Robert+B&rft.aulast=Levy-Clarke&rft.aufirst=Grace&rft.date=2005-08-01&rft.volume=19&rft.issue=4&rft.spage=739&rft.isbn=&rft.btitle=&rft.title=Hematology%2Foncology+clinics+of+North+America&rft.issn=08898588&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate. AN - 68456002; 16084394 AB - DNA polymerases occasionally insert the wrong nucleotide. For this error to become a mutation, the mispair must be extended. We report a structure of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of the polymerase active site. The structure of this complex indicates that the templating adenine of the mispair stacks with the primer terminus adenine while the templating (coding) cytosine is flipped out of the DNA helix. Soaking the crystals of the binary complex with dGTP resulted in crystals of a ternary substrate complex. In this case, the templating cytosine is observed within the DNA helix and forms Watson-Crick hydrogen bonds with the incoming dGTP. The adenine at the primer terminus has rotated into a syn-conformation to interact with the opposite adenine in a planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out of position for efficient nucleotide insertion. JF - Structure (London, England : 1993) AU - Batra, Vinod K AU - Beard, William A AU - Shock, David D AU - Pedersen, Lars C AU - Wilson, Samuel H AD - Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1225 EP - 1233 VL - 13 IS - 8 SN - 0969-2126, 0969-2126 KW - Nucleotides KW - 0 KW - DNA KW - 9007-49-2 KW - DNA Polymerase beta KW - EC 2.7.7.- KW - Index Medicus KW - Humans KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Mutation KW - Nucleotides -- metabolism KW - DNA -- metabolism KW - DNA Polymerase beta -- chemistry KW - DNA Polymerase beta -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68456002?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Structure+%28London%2C+England+%3A+1993%29&rft.atitle=Nucleotide-induced+DNA+polymerase+active+site+motions+accommodating+a+mutagenic+DNA+intermediate.&rft.au=Batra%2C+Vinod+K%3BBeard%2C+William+A%3BShock%2C+David+D%3BPedersen%2C+Lars+C%3BWilson%2C+Samuel+H&rft.aulast=Batra&rft.aufirst=Vinod&rft.date=2005-08-01&rft.volume=13&rft.issue=8&rft.spage=1225&rft.isbn=&rft.btitle=&rft.title=Structure+%28London%2C+England+%3A+1993%29&rft.issn=09692126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-19 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1ZJM; PDB; 1ZJN N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long-term effects of neonatal exposure to hydroxylated polychlorinated biphenyls in the BALB/cCrgl mouse. AN - 68446905; 16079073 AB - The neonatal mouse model has been a valuable tool in determining the long-term effects of early exposure to estrogenic agents in mammals. Using this model, we compared the effects of 2',4',6'-trichloro-4-biphenylol (OH-PCB-30) and 2',3',4',5'-tetrachloro-4-biphenylol (OH-PCB-61) as prototype estrogenic hydroxylated PCBs (OH-PCBs) because they are reported to exhibit relatively high estrogenic activity both in vivo and in vitro. The purpose of this study was to examine the relationship between estrogenicity and carcinogenicity of OH-PCB congeners. The OH-PCBs were tested individually and in combination to determine whether effects of combined OH-PCBs differed from those of these OH-PCBs alone. We evaluated the long-term effects of neonatal exposure to OH-PCBs with treatment doses that were based on the reported binding affinity of specific OH-PCB congeners to estrogen receptor alpha. BALB/cCrgl female mice were treated within 16 hr after birth by subcutaneous injections every 24 hr, for 5 days. The mice treated with OH-PCB-30 (200 microg/day) or 17beta-estradiol (5 microg/day) showed similar increased incidences of cervicovaginal (CV) tract carcinomas (43% and 47%, respectively). In addition, when mice were treated with OH-PCBs as a mixture, a change in the type of CV tract tumor was observed, shifting from predominantly squamous cell carcinomas to adenosquamous cell carcinoma. From our results, we conclude that the individual OH-PCBs tested were estrogenic and tumorigenic in mice when exposed during development of the reproductive tract. These data support the hypothesis that mixtures may act differently and unexpectedly than do individual compounds. JF - Environmental health perspectives AU - Martinez, Jeanelle M AU - Stephens, L Clifton AU - Jones, Lovell A AD - Department of Gynecologic Oncology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA. martine2@niehs.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1022 EP - 1026 VL - 113 IS - 8 SN - 0091-6765, 0091-6765 KW - Environmental Pollutants KW - 0 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Animals, Newborn KW - Animals KW - Environmental Pollutants -- toxicity KW - Mice KW - Mice, Inbred BALB C KW - Female KW - Hydroxylation KW - Vaginal Neoplasms -- chemically induced KW - Polychlorinated Biphenyls -- toxicity KW - Carcinoma, Squamous Cell -- chemically induced KW - Carcinoma, Adenosquamous -- chemically induced KW - Uterine Cervical Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68446905?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Long-term+effects+of+neonatal+exposure+to+hydroxylated+polychlorinated+biphenyls+in+the+BALB%2FcCrgl+mouse.&rft.au=Martinez%2C+Jeanelle+M%3BStephens%2C+L+Clifton%3BJones%2C+Lovell+A&rft.aulast=Martinez&rft.aufirst=Jeanelle&rft.date=2005-08-01&rft.volume=113&rft.issue=8&rft.spage=1022&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-17 N1 - Date created - 2005-08-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 1999 Jan;107(1):45-51 [9872716] Chemosphere. 1998 Oct-Nov;37(9-12):1845-53 [9828313] Toxicol Lett. 1998 Dec 28;102-103:331-5 [10022274] Environ Health Perspect. 1999 Aug;107 Suppl 4:639-49 [10421775] Sci Total Environ. 1999 Aug 15;233(1-3):141-61 [10492903] J Natl Cancer Inst. 1963 Aug;31:425-55 [14046632] Toxicology. 2005 Mar 30;208(3):377-87 [15695023] Toxicol Sci. 2005 Mar;84(1):49-62 [15601674] J Natl Cancer Inst. 1976 Nov;57(5):1057-62 [187788] J Natl Cancer Inst. 1976 Nov;57(5):1185-9 [187796] Arch Pathol Lab Med. 1977 Jan;101(1):1-5 [576195] Environ Res. 1978 Jul;16(1-3):123-30 [98321] Arch Toxicol. 1978 Dec 28;41(3):179-86 [104695] Cancer Res. 1979 Jul;39(7 Pt 1):2560-7 [445458] J Pediatr. 1984 Aug;105(2):315-20 [6431068] Arch Environ Contam Toxicol. 1985 Jan;14(1):51-7 [3919656] Environ Health Perspect. 1985 May;60:35-9 [2992924] IARC Sci Publ. 1985;(65):107-17 [4086078] Environ Res. 1986 Oct;41(1):14-22 [3093216] Am J Pathol. 2000 Apr;156(4):1289-98 [10751354] Int J Cancer. 2001 Feb 15;91(4):568-74 [11251983] Environ Toxicol Chem. 2001 Feb;20(2):351-8 [11351435] Environ Health Perspect. 2001 Jul;109(7):739-47 [11485874] Arch Environ Contam Toxicol. 2002 Jan;42(1):105-17 [11706375] Cancer Epidemiol Biomarkers Prev. 2002 Dec;11(12):1560-5 [12496044] Environ Sci Technol. 2003 Mar 1;37(5):832-9 [12666909] Environ Health Perspect. 2003 Apr;111(4):389-94 [12676588] Cancer. 2003 May 1;97(9):2196-202 [12712471] Environ Health Perspect. 2004 Aug;112(11):1208-12 [15289169] N Engl J Med. 1971 Apr 15;284(15):878-81 [5549830] Res Commun Chem Pathol Pharmacol. 1974 Sep;9(1):85-95 [4216060] J Steroid Biochem. 1975 May;6(5):673-6 [1186250] Cancer Res. 1977 Jan;37(1):67-75 [830422] Chem Biol Interact. 1986 Sep;59(2):173-84 [3769051] Mol Pharmacol. 1988 Jan;33(1):120-6 [3122017] Arch Environ Health. 1987 Nov-Dec;42(6):333-9 [3125795] Am J Epidemiol. 1989 Feb;129(2):395-406 [2492144] Cancer Res. 1990 Dec 1;50(23):7677-81 [2174729] J Natl Cancer Inst. 1994 Apr 20;86(8):589-99 [8145274] J Cardiovasc Surg (Torino). 1994 Dec;35(6):543-7 [7698972] Early Hum Dev. 1995 Apr 14;41(2):111-27 [7601016] Chemosphere. 1995 Aug;31(4):3017-23 [7552046] Arch Environ Contam Toxicol. 1995 Oct;29(3):334-43 [7487157] Environ Health Perspect. 1994 May;102(5):464-9 [8593850] Environ Health Perspect. 1995 Sep;103 Suppl 6:117-22 [8549457] Cancer Causes Control. 1995 Nov;6(6):551-66 [8580305] Environ Health Perspect. 1995 Oct;103 Suppl 7:141-5 [8593861] Biol Reprod. 1997 May;56(5):1147-57 [9160713] Environ Health Perspect. 1997 Apr;105 Suppl 3:577-81 [9167998] Toxicol Appl Pharmacol. 1997 Jul;145(1):111-23 [9221830] Environ Health Perspect. 1997 Oct;105(10):1030-2 [9349835] Toxicol Appl Pharmacol. 1997 Nov;147(1):93-100 [9356311] Ecotoxicol Environ Saf. 1997 Dec;38(3):281-5 [9469881] Toxicol Sci. 1998 Jan;41(1):62-76 [9520342] Chemosphere. 1997 Feb;34(4):835-48 [9569946] Am J Ind Med. 1998 Jul;34(1):6-14 [9617382] J Pediatr. 1999 Jan;134(1):33-41 [9880446] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Strategic planning: establishing need and clarifying motivation. AN - 68444424; 16079048 JF - Environmental health perspectives AU - Wilson, Samuel H AU - Schwartz, David A AD - wilson5@niehs.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1 VL - 113 IS - 8 SN - 0091-6765, 0091-6765 KW - Index Medicus KW - United States KW - Environmental Exposure KW - Organizational Objectives KW - Research KW - Planning Techniques KW - Environmental Health KW - National Institutes of Health (U.S.) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68444424?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Strategic+planning%3A+establishing+need+and+clarifying+motivation.&rft.au=Wilson%2C+Samuel+H%3BSchwartz%2C+David+A&rft.aulast=Wilson&rft.aufirst=Samuel&rft.date=2005-08-01&rft.volume=113&rft.issue=8&rft.spage=A506&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-17 N1 - Date created - 2005-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis. AN - 68441695; 16061934 AB - Zebrafish has become a favorite model organism not only in genetics and developmental biology, but also for the study of cancer, neuroscience and metabolism. However, strategies for reverse genetics in zebrafish are mostly limited to the use of antisense oligonucleotides, and therefore the development of other targeting methods is highly desirable. Here, we report an approach to gene targeting in this system in which single-stranded oligonucleotides and zebrafish Rad52 protein are employed. It has been proposed that a single-stranded oligonucleotide containing a mutation can be incorporated into the genome by annealing to the single-stranded region of the lagging strand of the replication fork. Rad52 is expected to accelerate the annealing step. In vitro experiments using purified truncated Rad52 proteins and replication protein A (RPA) showed that annealing of oligonucleotides is accelerated by Rad52 in the presence of RPA. We developed a simple and sensitive PCR-based method to detect point mutations in the genome. In exploratory experiments, we found that microinjection of single-stranded oligonucleotide targeted to a specific gene together with truncated Rad52 into zebrafish embryos resulted in a low level of recombinant copies in 3 of the 80 embryos tested under these conditions. JF - Nucleic acids research AU - Takahashi, Nobuhiro AU - Dawid, Igor B AD - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD 20892-2790, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1 VL - 33 IS - 13 KW - DNA-Binding Proteins KW - 0 KW - Nuclear Localization Signals KW - Oligonucleotides KW - Rad52 DNA Repair and Recombination Protein KW - Rad52 protein, zebrafish KW - Replication Protein A KW - Zebrafish Proteins KW - Index Medicus KW - Polymerase Chain Reaction KW - Animals KW - Sequence Alignment KW - Cells, Cultured KW - Point Mutation KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Microinjections KW - Cloning, Molecular KW - Mutagenesis, Site-Directed KW - Zebrafish Proteins -- isolation & purification KW - Oligonucleotides -- chemistry KW - DNA-Binding Proteins -- genetics KW - Zebrafish Proteins -- genetics KW - DNA-Binding Proteins -- isolation & purification KW - Zebrafish -- genetics KW - Zebrafish -- embryology KW - Oligonucleotides -- metabolism KW - Oligonucleotides -- administration & dosage KW - Zebrafish Proteins -- metabolism KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68441695?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+research&rft.atitle=Characterization+of+zebrafish+Rad52+and+replication+protein+A+for+oligonucleotide-mediated+mutagenesis.&rft.au=Takahashi%2C+Nobuhiro%3BDawid%2C+Igor+B&rft.aulast=Takahashi&rft.aufirst=Nobuhiro&rft.date=2005-08-01&rft.volume=33&rft.issue=13&rft.spage=e120&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+research&rft.issn=1362-4962&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - DQ021478; GENBANK; DQ021477 N1 - SuppNotes - Cited By: Mol Cell. 2002 Aug;10(2):359-71 [12191481] Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13492-7 [12370410] J Mol Med (Berl). 2002 Dec;80(12):770-81 [12483462] Mar Biotechnol (NY). 2003 Mar-Apr;5(2):174-84 [12876654] Nucleic Acids Res. 2003 Nov 15;31(22):6674-87 [14602928] Nucleic Acids Res. 2004;32(7):2093-101 [15087488] Curr Mol Med. 2004 Aug;4(5):445-63 [15267219] Mol Cell Biol. 1987 Jul;7(7):2329-34 [3302673] Proc Natl Acad Sci U S A. 1988 Jan;85(2):524-8 [2829192] Nucleic Acids Res. 1990 Feb 25;18(4):999-1005 [2179874] Mol Cell Biol. 1992 Jul;12(7):3050-9 [1320195] Genetics. 1992 Aug;131(4):811-9 [1325385] J Mol Biol. 1992 Sep 5;227(1):54-71 [1522601] Yeast. 1992 Nov;8(11):935-48 [1336288] J Biol Chem. 1994 Apr 15;269(15):11121-32 [8157639] Dev Dyn. 1995 Jul;203(3):253-310 [8589427] Nature. 1997 Jan 9;385(6612):176-81 [8990123] Development. 1996 Dec;123:1-36 [9007226] Proc Natl Acad Sci U S A. 1998 May 26;95(11):6049-54 [9600915] Genes Cells. 1998 Mar;3(3):145-56 [9619627] Annu Rev Biochem. 1998;67:721-51 [9759502] Genes Dev. 2004 Nov 15;18(22):2764-73 [15520275] Methods Cell Biol. 2004;76:151-60 [15602876] Methods Cell Biol. 2004;76:593-612 [15602894] Methods Cell Biol. 2004;77:69-90 [15602906] Methods Cell Biol. 2004;77:91-112 [15602907] Methods Cell Biol. 2004;77:113-9 [15602908] Methods Cell Biol. 2004;77:121-36 [15602909] Mech Dev. 2000 May;93(1-2):205-9 [10781958] Cell. 2000 Oct 27;103(3):449-56 [11081631] Gene Ther. 2001 Mar;8(5):391-9 [11313816] Nucleic Acids Res. 2001 Oct 15;29(20):4238-50 [11600713] Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14298-303 [11724925] Sci STKE. 2001 Mar 13;2001(73):pl1 [11752645] Mol Cell Biol. 2002 Jun;22(11):3852-63 [11997519] Methods Cell Biol. 2004;77:137-58 [15602910] Methods Cell Biol. 2004;77:403-11 [15602924] Nat Methods. 2004 Nov;1(2):141-7 [15782177] Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6742-6 [11381128] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long-latency afferent inhibition during selective finger movement. AN - 68439280; 15843479 AB - Stimulation of a peripheral nerve of a hand at rest modulates excitability in the motor cortex and, in particular, leads to inhibition when applied at an interval of approximately 200 ms (long-latency afferent inhibition; LAI). Surround inhibition (SI) is the process that inhibits neighboring muscles not involved in a particular task. The neuronal mechanisms of SI are not known, and it is possible that LAI might contribute to it. Using transcranial magnetic stimulation (TMS) with and without movement of the index finger, the motor-evoked potentials (MEPs) were measured of two functionally distinct target muscles of the hand (abductor digiti minimi muscle = ADM, 1st dorsal interosseus muscle = FDI). Electrical stimulation was applied 180 ms before TMS to either the fifth finger or the index finger. Both homotopic and heterotopic finger stimulation resulted in LAI without movement. With index finger movement, motor output further decreased with homo- and heterotopic stimulation in the ADM. In the moving FDI, however, there was no change with either homo- or heterotopic stimulation. Additionally, in the unstimulated movement trials, LAI increased with the amount of unintentional co-activation that occurred despite attempts to maintain the ADM at rest. However, with finger stimulation added, there were almost no increased MEPs despite co-activation. These findings suggest that LAI increases during movement and can enhance SI. JF - Journal of neurophysiology AU - Voller, Bernhard AU - St Clair Gibson, Alan AU - Lomarev, Mikhail AU - Kanchana, Sulada AU - Dambrosia, James AU - Dang, Nguyet AU - Hallett, Mark AD - Human Motor Control Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1115 EP - 1119 VL - 94 IS - 2 SN - 0022-3077, 0022-3077 KW - Index Medicus KW - Electric Stimulation -- methods KW - Analysis of Variance KW - Magnetics KW - Evoked Potentials -- radiation effects KW - Humans KW - Electromyography -- methods KW - Adult KW - Evoked Potentials -- physiology KW - Middle Aged KW - Dose-Response Relationship, Radiation KW - Time Factors KW - Male KW - Female KW - Reaction Time -- radiation effects KW - Afferent Pathways -- radiation effects KW - Neural Inhibition -- radiation effects KW - Movement -- physiology KW - Fingers -- physiology KW - Afferent Pathways -- physiology KW - Neural Inhibition -- physiology KW - Reaction Time -- physiology KW - Movement -- radiation effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68439280?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Long-latency+afferent+inhibition+during+selective+finger+movement.&rft.au=Voller%2C+Bernhard%3BSt+Clair+Gibson%2C+Alan%3BLomarev%2C+Mikhail%3BKanchana%2C+Sulada%3BDambrosia%2C+James%3BDang%2C+Nguyet%3BHallett%2C+Mark&rft.aulast=Voller&rft.aufirst=Bernhard&rft.date=2005-08-01&rft.volume=94&rft.issue=2&rft.spage=1115&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Presynaptic angiotensin II AT1 receptors enhance inhibitory and excitatory synaptic neurotransmission to motoneurons and other ventral horn neurons in neonatal rat spinal cord. AN - 68438130; 16061493 AB - In neonatal spinal cord, we previously reported that exogenous angiotensin II (ANG II) acts at postsynaptic AT1 receptors to depolarize neonatal rat spinal ventral horn neurons in vitro. This study evaluated an associated increase in synaptic activity. Patch clamp recordings revealed that 38/81 thoracolumbar (T7-L5) motoneurons responded to bath applied ANG II (0.3-1 microM; 30 s) with a prolonged (5-10 min) and reversible increase in spontaneous postsynaptic activity, selectively blockable with Losartan (n = 5) but not PD123319 (n = 5). ANG-II-induced events included both spontaneous inhibitory (IPSCs; n = 6) and excitatory postsynaptic currents (EPSCs; n = 5). While most ANG induced events were tetrodotoxin-sensitive, ANG induced a significant tetrodotoxin-resistant increase in frequency but not amplitude of miniature IPSCs (n = 7/13 cells) and EPSCs (n = 2/7 cells). In 35/77 unidentified neurons, ANG II also induced a tetrodotoxin-sensitive and prolonged increase in their spontaneous synaptic activity that featured both IPSCs (n = 5) and EPSCs (n = 4) when tested in the presence of selective amino acid receptor antagonists. When tested in the presence of tetrodotoxin, ANG II was noted to induce a significant increase in the frequency but not the amplitude of mIPSCs (n = 9) and mEPSCs (n = 8). ANG also increased spontaneous motor activity from isolated mouse lumbar ventral rootlets. Collectively, these observations support the existence of a wide pre- and postsynaptic distribution of ANG II AT1 receptors in neonatal ventral spinal cord that are capable of influencing both inhibitory and excitatory neurotransmission. JF - Journal of neurophysiology AU - Oz, Murat AU - Yang, Keun-Hang AU - O'donovan, Michael J AU - Renaud, Leo P AD - National Institute on Drug Abuse, Cellular Neurobiology Branch, National Institutes of Health, Baltimore, MD 21224, USA. moz@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1405 EP - 1412 VL - 94 IS - 2 SN - 0022-3077, 0022-3077 KW - Excitatory Amino Acid Antagonists KW - 0 KW - GABA Antagonists KW - Quinoxalines KW - Receptor, Angiotensin, Type 1 KW - Angiotensin II KW - 11128-99-7 KW - 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline KW - 118876-58-7 KW - Tetrodotoxin KW - 4368-28-9 KW - 2-Amino-5-phosphonovalerate KW - 76726-92-6 KW - Strychnine KW - H9Y79VD43J KW - Bicuculline KW - Y37615DVKC KW - Index Medicus KW - Patch-Clamp Techniques -- methods KW - Bicuculline -- pharmacology KW - Animals KW - Drug Interactions KW - Electric Stimulation -- methods KW - Dose-Response Relationship, Drug KW - GABA Antagonists -- pharmacology KW - Angiotensin II -- pharmacology KW - Excitatory Amino Acid Antagonists -- pharmacology KW - Rats KW - Animals, Newborn KW - Rats, Sprague-Dawley KW - Laminectomy -- methods KW - 2-Amino-5-phosphonovalerate -- pharmacology KW - In Vitro Techniques KW - Strychnine -- pharmacology KW - Tetrodotoxin -- pharmacology KW - Quinoxalines -- pharmacology KW - Male KW - Female KW - Motor Neurons -- physiology KW - Excitatory Postsynaptic Potentials -- drug effects KW - Motor Neurons -- classification KW - Receptor, Angiotensin, Type 1 -- physiology KW - Motor Neurons -- cytology KW - Neural Inhibition -- drug effects KW - Synaptic Transmission -- physiology KW - Neural Inhibition -- physiology KW - Excitatory Postsynaptic Potentials -- physiology KW - Spinal Cord -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68438130?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Presynaptic+angiotensin+II+AT1+receptors+enhance+inhibitory+and+excitatory+synaptic+neurotransmission+to+motoneurons+and+other+ventral+horn+neurons+in+neonatal+rat+spinal+cord.&rft.au=Oz%2C+Murat%3BYang%2C+Keun-Hang%3BO%27donovan%2C+Michael+J%3BRenaud%2C+Leo+P&rft.aulast=Oz&rft.aufirst=Murat&rft.date=2005-08-01&rft.volume=94&rft.issue=2&rft.spage=1405&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. AN - 68437118; 16041368 AB - Centrosome duplication is tightly controlled during faithful cell division, and unnecessary reduplication can lead to supernumerary centrosomes and multipolar spindles that are associated with most human cancer cells. In addition to nucleocytoplasmic transport, the Ran-Crm1 network is involved in regulating centrosome duplication to ensure the formation of a bipolar spindle. Here, we discover that nucleophosmin (NPM) may be a Ran-Crm1 substrate that controls centrosome duplication. NPM contains a functional nuclear export signal (NES) that is responsible for both its nucleocytoplasmic shuttling and its association with centrosomes, which are Ran-Crm1-dependent as they are sensitive to Crm1-specific nuclear export inhibition, either by leptomycin B (LMB) or by the expression of a Ran-binding protein, RanBP1. Notably, LMB treatment induces premature centrosome duplication in quiescent cells, which coincides with NPM dissociation from centrosomes. Moreover, deficiency of NPM by RNA interference results in supernumerary centrosomes, which can be reversed by reintroducing wild-type but not NES-mutated NPM. Mutation of a potential proline-dependent kinase phosphorylation site at residue 95, from threonine to aspartic acid (T95D) within the NES motif, abolishes NPM association and inhibition of centrosome duplication. Our results are consistent with the hypothesis that the Ran-Crm1 complex may promote a local enrichment of NPM on centrosomes, thereby preventing centrosome reduplication. JF - Nature cell biology AU - Wang, Wei AU - Budhu, Anuradha AU - Forgues, Marshonna AU - Wang, Xin Wei AD - Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute/NIH, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 823 EP - 830 VL - 7 IS - 8 SN - 1465-7392, 1465-7392 KW - Fatty Acids, Unsaturated KW - 0 KW - Karyopherins KW - Nuclear Proteins KW - RNA, Small Interfering KW - Receptors, Cytoplasmic and Nuclear KW - Trans-Activators KW - exportin 1 protein KW - hepatitis B virus X protein KW - ran-binding protein 1 KW - nucleophosmin KW - 117896-08-9 KW - Threonine KW - 2ZD004190S KW - ran GTP-Binding Protein KW - EC 3.6.5.2 KW - leptomycin B KW - Y031I2N1EO KW - Index Medicus KW - Animals KW - Threonine -- metabolism KW - Threonine -- genetics KW - Cell Nucleus -- metabolism KW - Humans KW - Interphase -- physiology KW - Protein Binding -- physiology KW - Phosphorylation KW - Trans-Activators -- genetics KW - Molecular Sequence Data KW - Binding Sites -- genetics KW - Sequence Homology, Amino Acid KW - Protein Transport -- physiology KW - HeLa Cells KW - Active Transport, Cell Nucleus -- drug effects KW - Protein Transport -- drug effects KW - Amino Acid Sequence KW - Mice KW - RNA, Small Interfering -- genetics KW - Binding Sites -- physiology KW - Gene Expression -- genetics KW - Cell Fusion KW - Transfection KW - Active Transport, Cell Nucleus -- physiology KW - Mutation -- genetics KW - Fatty Acids, Unsaturated -- pharmacology KW - Cell Line KW - Receptors, Cytoplasmic and Nuclear -- physiology KW - Nuclear Proteins -- genetics KW - Karyopherins -- physiology KW - ran GTP-Binding Protein -- genetics KW - ran GTP-Binding Protein -- physiology KW - Nuclear Proteins -- metabolism KW - Nuclear Proteins -- physiology KW - Centrosome -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68437118?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+cell+biology&rft.atitle=Temporal+and+spatial+control+of+nucleophosmin+by+the+Ran-Crm1+complex+in+centrosome+duplication.&rft.au=Wang%2C+Wei%3BBudhu%2C+Anuradha%3BForgues%2C+Marshonna%3BWang%2C+Xin+Wei&rft.aulast=Wang&rft.aufirst=Wei&rft.date=2005-08-01&rft.volume=7&rft.issue=8&rft.spage=823&rft.isbn=&rft.btitle=&rft.title=Nature+cell+biology&rft.issn=14657392&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A new direction for gene therapy: intrathymic T cell-specific lentiviral gene transfer. AN - 68436682; 16075048 AB - Reports of neoplasia related to insertional activation of protooncogenes by retroviral vectors have raised serious safety concerns in the field of gene therapy. Modification of current approaches is urgently required to minimize the deleterious consequences of insertional mutagenesis. In this issue of the JCI, Adjali and colleagues report on their treatment of SCID mice lacking the 70-kDa protein tyrosine kinase, ZAP-70, with direct intrathymic injection of a ZAP-70-expressing T cell-specific lentiviral vector, which resulted in T cell reconstitution. Using lentiviral vectors and in situ gene transfer may represent a safer approach than using retroviral vectors for ex vivo gene transfer into HSCs, avoiding 3 factors potentially linked to leukemogenesis, namely HSC targets, ex vivo transduction and expansion, and standard Moloney leukemia virus-based retroviral vectors. JF - The Journal of clinical investigation AU - Seggewiss, Ruth AU - Dunbar, Cynthia E AD - National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 2064 EP - 2067 VL - 115 IS - 8 SN - 0021-9738, 0021-9738 KW - Protein-Tyrosine Kinases KW - EC 2.7.10.1 KW - ZAP-70 Protein-Tyrosine Kinase KW - EC 2.7.10.2 KW - ZAP70 protein, human KW - Zap70 protein, mouse KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Humans KW - Mice KW - Mice, SCID KW - Neoplasms -- genetics KW - Protein-Tyrosine Kinases -- genetics KW - Severe Combined Immunodeficiency -- enzymology KW - Genetic Therapy -- adverse effects KW - Severe Combined Immunodeficiency -- therapy KW - Lentivirus KW - Genetic Therapy -- methods KW - Thymus Gland -- enzymology KW - Protein-Tyrosine Kinases -- metabolism KW - T-Lymphocytes -- enzymology KW - Severe Combined Immunodeficiency -- genetics KW - Transduction, Genetic -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68436682?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+clinical+investigation&rft.atitle=A+new+direction+for+gene+therapy%3A+intrathymic+T+cell-specific+lentiviral+gene+transfer.&rft.au=Seggewiss%2C+Ruth%3BDunbar%2C+Cynthia+E&rft.aulast=Seggewiss&rft.aufirst=Ruth&rft.date=2005-08-01&rft.volume=115&rft.issue=8&rft.spage=2064&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+clinical+investigation&rft.issn=00219738&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: N Engl J Med. 2000 May 4;342(18):1325-32 [10793165] BMC Immunol. 2004 Aug 19;5:18 [15318949] Mol Ther. 2000 Mar;1(3):285-93 [10933944] Science. 2000 Apr 28;288(5466):669-72 [10784449] J Immunol. 1994 May 15;152(10):4758-66 [8176201] N Engl J Med. 1996 Nov 21;335(21):1563-7 [8900089] J Pediatr. 1997 Mar;130(3):378-87 [9063412] Immunity. 1997 Jun;6(6):663-71 [9208839] J Exp Med. 1998 Jul 20;188(2):393-8 [9670051] Semin Hematol. 1998 Oct;35(4):310-20 [9801260] PLoS Biol. 2004 Dec;2(12):e423 [15550989] J Clin Invest. 2005 Aug;115(8):2287-95 [16075064] J Clin Invest. 2001 Aug;108(3):447-55 [11489938] Blood. 2002 Feb 15;99(4):1165-73 [11830462] N Engl J Med. 2002 Apr 18;346(16):1185-93 [11961146] Cell. 2002 Aug 23;110(4):521-9 [12202041] Lancet. 2003 Feb 15;361(9357):553-60 [12598139] Science. 2003 Oct 17;302(5644):415-9 [14564000] Annu Rev Immunol. 2004;22:625-55 [15032591] Comment On: J Clin Invest. 2005 Aug;115(8):2287-95 [16075064] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Increased tumorigenicity and sensitivity to ionizing radiation upon loss of chromosomal protein HMGN1. AN - 68435942; 16061652 AB - We report that loss of HMGN1, a nucleosome-binding protein that alters the compaction of the chromatin fiber, increases the cellular sensitivity to ionizing radiation and the tumor burden of mice. The mortality and tumor burden of ionizing radiation-treated Hmgn1-/- mice is higher than that of their Hmgn1+/+ littermates. Hmgn1-/- fibroblasts have an altered G2-M checkpoint activation and are hypersensitive to ionizing radiation. The ionizing radiation hypersensitivity and the aberrant G2-M checkpoint activation of Hmgn1-/- fibroblasts can be reverted by transfections with plasmids expressing wild-type HMGN1, but not with plasmids expressing mutant HMGN proteins that do not bind to chromatin. Transformed Hmgn1-/- fibroblasts grow in soft agar and produce tumors in nude mice with a significantly higher efficiency than Hmgn1+/+ fibroblasts, suggesting that loss of HMGN1 protein disrupts cellular events controlling proliferation and growth. Hmgn1-/- mice have a higher incidence of multiple malignant tumors and metastases than their Hmgn1+/+ littermates. We suggest that HMGN1 optimizes the cellular response to ionizing radiation and to other tumorigenic events; therefore, loss of this protein increases the tumor burden in mice. JF - Cancer research AU - Birger, Yehudit AU - Catez, Frédéric AU - Furusawa, Takashi AU - Lim, Jae-Hwan AU - Prymakowska-Bosak, Marta AU - West, Katherine L AU - Postnikov, Yuri V AU - Haines, Diana C AU - Bustin, Michael AD - Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 6711 EP - 6718 VL - 65 IS - 15 SN - 0008-5472, 0008-5472 KW - HMGN1 Protein KW - 0 KW - Index Medicus KW - Animals KW - G2 Phase -- radiation effects KW - Mice, Nude KW - Mice KW - Fibroblasts -- radiation effects KW - Fibroblasts -- cytology KW - Male KW - Female KW - Cell Division -- radiation effects KW - HMGN1 Protein -- physiology KW - HMGN1 Protein -- metabolism KW - Neoplasms, Radiation-Induced -- pathology KW - Cell Transformation, Neoplastic -- radiation effects KW - Cell Transformation, Neoplastic -- metabolism KW - Neoplasms, Radiation-Induced -- metabolism KW - Radiation Tolerance -- physiology KW - HMGN1 Protein -- deficiency UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68435942?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Increased+tumorigenicity+and+sensitivity+to+ionizing+radiation+upon+loss+of+chromosomal+protein+HMGN1.&rft.au=Birger%2C+Yehudit%3BCatez%2C+Fr%C3%A9d%C3%A9ric%3BFurusawa%2C+Takashi%3BLim%2C+Jae-Hwan%3BPrymakowska-Bosak%2C+Marta%3BWest%2C+Katherine+L%3BPostnikov%2C+Yuri+V%3BHaines%2C+Diana+C%3BBustin%2C+Michael&rft.aulast=Birger&rft.aufirst=Yehudit&rft.date=2005-08-01&rft.volume=65&rft.issue=15&rft.spage=6711&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: EMBO J. 1999 Dec 1;18(23):6585-98 [10581233] Nature. 2000 Apr 6;404(6778):604-9 [10766243] Nature. 2000 Nov 23;408(6811):433-9 [11100718] Mol Cell Biol. 2001 Aug;21(15):5169-78 [11438671] Trends Biochem Sci. 2001 Jul;26(7):431-7 [11440855] Mol Cell. 2001 Jul;8(1):57-69 [11511360] Curr Opin Oncol. 2001 Nov;13(6):477-83 [11673688] Curr Opin Genet Dev. 2002 Feb;12(1):73-9 [11790558] EMBO Rep. 2002 Jan;3(1):28-33 [11799057] Curr Opin Genet Dev. 2002 Apr;12(2):162-9 [11893489] Science. 2002 May 3;296(5569):922-7 [11934988] EMBO Rep. 2002 Aug;3(8):760-6 [12151335] Oncogene. 2002 Sep 9;21(40):6228-33 [12214253] Nat Cell Biol. 2002 Dec;4(12):993-7 [12447390] Nature. 2003 Jan 30;421(6922):499-506 [12556884] Nat Rev Cancer. 2003 Mar;3(3):155-68 [12612651] EMBO J. 2003 Apr 1;22(7):1665-75 [12660172] Nat Cell Biol. 2003 Jul;5(7):675-9 [12792649] Cell. 2003 Aug 8;114(3):359-70 [12914700] Cell. 2003 Aug 8;114(3):371-83 [12914701] Nat Cell Biol. 2004 Feb;6(2):168-70 [14755273] Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1427-8 [14757822] Genes Dev. 2004 Mar 15;18(6):602-16 [15075289] J Exp Med. 2004 Jun 21;199(12):1671-7 [15197225] Mol Cell. 2004 Aug 27;15(4):573-84 [15327773] Science. 1994 Aug 5;265(5173):796-9 [8047885] EMBO J. 1995 Apr 3;14(7):1478-89 [7729423] Genes Dev. 1995 Aug 15;9(16):1978-91 [7649479] Cell. 1997 Nov 28;91(5):649-59 [9393858] J Biol Chem. 1998 Apr 17;273(16):9409-14 [9545265] Prog Nucleic Acid Res Mol Biol. 1999;62:227-55 [9932456] Mol Cell Biol. 1999 Aug;19(8):5237-46 [10409715] J Cell Biol. 1999 Sep 6;146(5):905-16 [10477747] Nature. 2004 Nov 18;432(7015):316-23 [15549093] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Medication development for addictive disorders: the state of the science. AN - 68094340; 16055764 AB - In 1989, the National Institute on Drug Abuse (NIDA) established its Medications Development Program. This program has concentrated on developing pharmacotherapies for opiate and cocaine dependence and, more recently, for methamphetamine and cannabis dependence. The major goals of this program are to optimize existing treatments and to expand treatment options for physicians and patients. This review will concentrate on the development of pharmacotherapies for the following substance abuse disorders: opiate, cocaine, methamphetamine, and cannabis dependence. Left untreated, opiate and stimulant dependence are responsible for significant morbidity and mortality. For example, use of illicit opiates is associated with an increased risk of hepatitis C infection, HIV infection, and other medical consequences, e.g., an overdose. The NIDA Medications Development Program has had success in developing, with pharmaceutical partners, levomethadyl acetate, buprenorphine, and buprenorphine/naloxone for opiate dependence. Moreover, several marketed medications have shown promise in reducing cocaine use. Of interest, these medications likely operate through diverse neurochemical mechanisms, suggesting that combination therapy may be a rational next step that could increase treatment gains further in cocaine-dependent patients. The Medications Development Program has also identified multiple neuronal mechanisms that are altered by chronic administration of drugs of abuse. Advances in neuroscience have identified changes in conditioned cueing, drug priming, stress-induced increases in drug intake, and reduced frontal inhibitory mechanisms as all being possible for the development of, maintenance of, and possible relapse to, addiction. Potential medications that modulate these mechanisms are highlighted. JF - The American journal of psychiatry AU - Vocci, Frank J AU - Acri, Jane AU - Elkashef, Ahmed AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, 6001 Executive Blvd., Rm. 4133, MSC 9551, Bethesda, MD 20892-9551, USA. fv6k@nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1432 EP - 1440 VL - 162 IS - 8 SN - 0002-953X, 0002-953X KW - Naloxone KW - 36B82AMQ7N KW - Buprenorphine KW - 40D3SCR4GZ KW - Methadyl Acetate KW - L59OC40KWJ KW - Dopamine KW - VTD58H1Z2X KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Animals KW - Buprenorphine -- therapeutic use KW - Chemistry, Pharmaceutical KW - Humans KW - National Institutes of Health (U.S.) -- organization & administration KW - Cocaine-Related Disorders -- drug therapy KW - Clinical Trials as Topic KW - Dopamine -- physiology KW - Opioid-Related Disorders -- drug therapy KW - Drug Design KW - Drug Therapy, Combination KW - Drug Industry -- organization & administration KW - Naloxone -- therapeutic use KW - Methadyl Acetate -- therapeutic use KW - Program Development -- methods KW - Secondary Prevention KW - Substance-Related Disorders -- physiopathology KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68094340?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Medication+development+for+addictive+disorders%3A+the+state+of+the+science.&rft.au=Vocci%2C+Frank+J%3BAcri%2C+Jane%3BElkashef%2C+Ahmed&rft.aulast=Vocci&rft.aufirst=Frank&rft.date=2005-08-01&rft.volume=162&rft.issue=8&rft.spage=1432&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Developments in the epidemiology of drug use and drug use disorders. AN - 68092891; 16055770 AB - The past 30 years of research on the epidemiology of drug use, drug use disorders, and related conditions, such as HIV, has provided major insight into these conditions. Drug use peaked in the late 1970s, decreased across the 1980s, increased in the 1990s, and has remained stable during the past few years. Within this broad pattern, specific epidemics of crack cocaine, amphetamines, club drugs (such as Ecstasy), heroin, and prescription opioids and associated epidemics of HIV and other infectious diseases have been identified and tracked. Besides major accomplishments in surveillance, the epidemiology of drug use and drug use disorders has traditionally focused on identifying risk factors at the individual (genetic factors, high-risk behaviors), family (child abuse), neighborhood (high availability of drugs), and societal (policies and laws) levels as domains of influence, not as components of interrelated processes. Research includes careful cross-sectional and longitudinal observational studies as well as clinical epidemiological experiments in which prevention interventions test specific etiological theories. Building on this background, the next challenges for the epidemiology of drug use and drug use disorders will be to link individual vulnerabilities with specific environmental factors by using multilevel methodological approaches. For example, what are the environmental factors that interact with individual vulnerabilities to produce drug addictions and drug consequences such as HIV? Research in genetic epidemiology has demonstrated the potential for studies of interactions of genetic and environmental factors. The field needs to focus on linking science with epidemiology to make progress in understanding these complex health conditions. JF - The American journal of psychiatry AU - Compton, Wilson M AU - Thomas, Yonette F AU - Conway, Kevin P AU - Colliver, James D AD - Division of Epidemiology, Services, and Prevention Research, National Institute on Drug Abuse, 6001 Executive Blvd., MSC 9589, Bethesda, MD 20892-9589, USA. wcompton@nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1494 EP - 1502 VL - 162 IS - 8 SN - 0002-953X, 0002-953X KW - Abridged Index Medicus KW - Index Medicus KW - Students -- statistics & numerical data KW - Risk-Taking KW - Epidemiologic Methods KW - Humans KW - HIV Infections -- etiology KW - Child KW - Comorbidity KW - Ethnic Groups -- statistics & numerical data KW - Epidemiologic Studies KW - Risk Factors KW - Adolescent Behavior -- psychology KW - Health Surveys KW - Marijuana Abuse -- psychology KW - Marijuana Abuse -- epidemiology KW - Disease Outbreaks -- statistics & numerical data KW - Adolescent KW - United States -- epidemiology KW - HIV Infections -- epidemiology KW - Social Environment KW - Substance-Related Disorders -- psychology KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68092891?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Developments+in+the+epidemiology+of+drug+use+and+drug+use+disorders.&rft.au=Compton%2C+Wilson+M%3BThomas%2C+Yonette+F%3BConway%2C+Kevin+P%3BColliver%2C+James+D&rft.aulast=Compton&rft.aufirst=Wilson&rft.date=2005-08-01&rft.volume=162&rft.issue=8&rft.spage=1494&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Monoamine oxidase-A is a major target gene for glucocorticoids in human skeletal muscle cells. AN - 68088781; 15946989 AB - Skeletal myopathy is a common complication of endogenous and exogenous glucocorticoid excess, yet its pathogenetic mechanisms remain unclear. There is accumulating evidence that mitochondrial dysfunction and oxidative stress are involved in this process. To explore the glucocorticoid-induced transcriptional adaptations that may affect mitochondrial function in skeletal muscle, we studied gene expression profiles in dexamethasone-treated primary human skeletal myocytes using a cDNA microarray, which contains 501 mitochondria-related genes. We found that monoamine oxidase A (MAO-A) was the most significantly up-regulated gene. MAO-A is the primary enzyme metabolizing catecholamines and dietary amines, and its role in skeletal muscle remains largely unexplored. Dexamethasone induced dose- and time-dependent increases of MAO-A gene and protein expression, while its effects on MAO-B were minimal. Both the glucocorticoid receptor (GR) and the Sp1 transcription factor were required for dexamethasone-induced MAO-A mRNA expression, as blockade of the GR with RU 486 or ablation of Sp1 binding with mithramycin abrogated MAO-A mRNA induction. The observed dexamethasone effect was biologically functional, as this steroid significantly increased MAO-mediated hydrogen peroxide production. We suggest that MAO-A-mediated oxidative stress can lead to cell damage, representing a novel pathogenetic mechanism for glucocorticoid-induced myopathy and a potential target for therapeutic intervention. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Manoli, Irini AU - Le, Hanh AU - Alesci, Salvatore AU - McFann, Kimberly K AU - Su, Yan A AU - Kino, Tomoshige AU - Chrousos, George P AU - Blackman, Marc R AD - Endocrine Section, Laboratory of Clinical Investigation, NCCAM, NIH, Bethesda, Maryland 20892, USA. manolii@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1359 EP - 1361 VL - 19 IS - 10 KW - Monoamine Oxidase Inhibitors KW - 0 KW - RNA, Messenger KW - Receptors, Glucocorticoid KW - Sp1 Transcription Factor KW - Dexamethasone KW - 7S5I7G3JQL KW - Hydrogen Peroxide KW - BBX060AN9V KW - Monoamine Oxidase KW - EC 1.4.3.4 KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Sp1 Transcription Factor -- physiology KW - Dose-Response Relationship, Drug KW - Humans KW - Hydrogen Peroxide -- metabolism KW - RNA, Messenger -- analysis KW - Receptors, Glucocorticoid -- physiology KW - Transcriptional Activation KW - Monoamine Oxidase Inhibitors -- pharmacology KW - Promoter Regions, Genetic KW - Muscular Diseases -- chemically induced KW - Cells, Cultured KW - Adult KW - Adolescent KW - Muscle Fibers, Skeletal -- enzymology KW - Male KW - Muscle Fibers, Skeletal -- drug effects KW - Dexamethasone -- toxicity KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Monoamine Oxidase -- biosynthesis KW - Muscle, Skeletal -- metabolism KW - Muscle, Skeletal -- drug effects KW - Monoamine Oxidase -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68088781?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=Monoamine+oxidase-A+is+a+major+target+gene+for+glucocorticoids+in+human+skeletal+muscle+cells.&rft.au=Manoli%2C+Irini%3BLe%2C+Hanh%3BAlesci%2C+Salvatore%3BMcFann%2C+Kimberly+K%3BSu%2C+Yan+A%3BKino%2C+Tomoshige%3BChrousos%2C+George+P%3BBlackman%2C+Marc+R&rft.aulast=Manoli&rft.aufirst=Irini&rft.date=2005-08-01&rft.volume=19&rft.issue=10&rft.spage=1359&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-06 N1 - Date created - 2005-07-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Secondhand smoke exposure in early life and the risk of breast cancer among never smokers (United States). AN - 68087132; 16049807 AB - Evidence is increasing that some early life exposures affect breast cancer risk. Exposure to secondhand smoke (SHS) during childhood may be one such exposure. As part of the WEB Study (Western New York Exposures and Breast Cancer Study), we conducted a population-based, case-control study with 1166 women aged 35 to 79 diagnosed with histologically confirmed, primary, incident breast cancer. Controls (n = 2105) were randomly selected from the Department of Motor Vehicles driver's license list ( age 65). Participants were queried regarding household and workplace SHS exposure. Person-years of lifetime cumulative SHS exposure were computed as well as cumulative exposure up to 21 years of age. Unconditional logistic regression adjusting for potential confounders was used to calculate odds ratios (OR) and 95% confidence intervals (95% CI). Lifetime cumulative exposure to household SHS was not associated with an increase in breast cancer risk for premenopausal (OR = 1.17, 95% CI = 0.54-2.56) or postmenopausal (OR = 1.29; 95% CI = 0.82-2.01) women. Neither was risk increased among women exposed to SHS before the age of 21 or at the time of birth, menarche, or a women's first birth. In this study, exposure to SHS either in adult or early life does not appear to be associated with the risk of breast cancer. JF - Cancer causes & control : CCC AU - Bonner, Matthew R AU - Nie, Jing AU - Han, Daikwon AU - Vena, John E AU - Rogerson, Peter AU - Muti, Paola AU - Trevisan, Maurizio AU - Edge, Stephen B AU - Freudenheim, Jo L AD - Department of Social and Preventive Medicine, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY 14214, USA. bonnerm@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 683 EP - 689 VL - 16 IS - 6 SN - 0957-5243, 0957-5243 KW - Tobacco Smoke Pollution KW - 0 KW - Index Medicus KW - Risk Factors KW - Humans KW - Adult KW - Case-Control Studies KW - Aged KW - Middle Aged KW - New York -- epidemiology KW - Menopause KW - Female KW - Tobacco Smoke Pollution -- adverse effects KW - Environmental Exposure KW - Breast Neoplasms -- etiology KW - Breast Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68087132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+causes+%26+control+%3A+CCC&rft.atitle=Secondhand+smoke+exposure+in+early+life+and+the+risk+of+breast+cancer+among+never+smokers+%28United+States%29.&rft.au=Bonner%2C+Matthew+R%3BNie%2C+Jing%3BHan%2C+Daikwon%3BVena%2C+John+E%3BRogerson%2C+Peter%3BMuti%2C+Paola%3BTrevisan%2C+Maurizio%3BEdge%2C+Stephen+B%3BFreudenheim%2C+Jo+L&rft.aulast=Bonner&rft.aufirst=Matthew&rft.date=2005-08-01&rft.volume=16&rft.issue=6&rft.spage=683&rft.isbn=&rft.btitle=&rft.title=Cancer+causes+%26+control+%3A+CCC&rft.issn=09575243&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-20 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Idiopathic intracranial hypertension following kidney transplantation: a case report and review of the literature. AN - 68083924; 16048612 AB - A pediatric kidney transplant recipient receiving tacrolimus for immunosuppression experienced symptoms consistent with idiopathic intracranial hypertension. The diagnosis of idiopathic intracranial hypertension and possible secondary causes of intracranial hypertension are reviewed in association with the patient's clinical course. Treatment options for the reversal of intracranial hypertension are summarized. Because of the complexity of associated conditions in kidney transplant recipients, symptoms of persistent headaches, visual changes and nausea and vomiting should be promptly investigated by fundoscopic examination in the setting of immunosuppression therapy to prevent vision loss. JF - Pediatric transplantation AU - Chamberlain, Christine E AU - Fitzgibbon, Edmond AU - Wassermann, Eric M AU - Butman, John A AU - Kettl, David AU - Hale, Doug AU - Kirk, Allan D AU - Mannon, Roslyn B AD - Pharmacy Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. cchamberla@nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 545 EP - 550 VL - 9 IS - 4 SN - 1397-3142, 1397-3142 KW - Tacrolimus KW - WM0HAQ4WNM KW - Index Medicus KW - Humans KW - Magnetic Resonance Angiography KW - Overweight KW - Child KW - Female KW - Tacrolimus -- adverse effects KW - Kidney Transplantation KW - Intracranial Hypertension -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68083924?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatric+transplantation&rft.atitle=Idiopathic+intracranial+hypertension+following+kidney+transplantation%3A+a+case+report+and+review+of+the+literature.&rft.au=Chamberlain%2C+Christine+E%3BFitzgibbon%2C+Edmond%3BWassermann%2C+Eric+M%3BButman%2C+John+A%3BKettl%2C+David%3BHale%2C+Doug%3BKirk%2C+Allan+D%3BMannon%2C+Roslyn+B&rft.aulast=Chamberlain&rft.aufirst=Christine&rft.date=2005-08-01&rft.volume=9&rft.issue=4&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Pediatric+transplantation&rft.issn=13973142&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-09-12 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Safety, tolerability, and pharmacokinetics of Micafungin (FK463) in febrile neutropenic pediatric patients. AN - 68081060; 16048942 AB - Micafungin (FK463) is a new parenteral echinocandin. A multicenter, phase I, open-label, sequential-group dose escalation study was conducted to assess the safety, tolerability, and pharmacokinetics of micafungin in neutropenic pediatric patients. A total of 77 patients stratified by age (2 to 12 and 13 to 17 years) received micafungin. Therapy was initiated at 0.5 mg/kg per day and escalated to higher dose levels of 1.0, 1.5, 2.0, 3.0, and 4.0 mg/kg per day. Micafungin was administered within 24 h of initiating broad-spectrum antibacterial antibiotics for the new onset of fever and neutropenia. The most common overall adverse events in the study population were diarrhea (19.5%), epistaxis (18.2%), abdominal pain (16.9%), and headache (16.9%). Nine patients (12%) experienced adverse events considered by the investigator to be possibly related to the study drug. The most common related events were diarrhea, vomiting, and headache, all occurring in two patients each. There was no evidence of a dose-limiting toxicity as defined within the prespecified criteria of this clinical protocol. There was one death during the study due to septic shock. The pharmacokinetic profiles for micafungin over the 0.5- to 4.0-mg/kg dose range demonstrated dose linearity. Clearance, volume of distribution, and half-life remained relatively constant over the dose range and did not change with repeated administration. The overall plasma pharmacokinetic profile was similar to that observed in adults. However, there was an inverse relation between age and clearance. For patients 2 to 8 years old, clearance was approximately 1.35 times that of patients >/=9 years of age. In summary, micafungin over a dosage range between 0.5 and 4.0 mg/kg/day in 77 febrile neutropenic pediatric patients displayed linear pharmacokinetics and increased clearance as a function of decreasing age. JF - Antimicrobial agents and chemotherapy AU - Seibel, Nita L AU - Schwartz, Cindy AU - Arrieta, Antonio AU - Flynn, Patricia AU - Shad, Aziza AU - Albano, Edith AU - Keirns, James AU - Lau, Wendi M AU - Facklam, David P AU - Buell, Donald N AU - Walsh, Thomas J AD - Pediatric Oncology Branch, National Cancer Institute, Bldg. 10, Rm. 13N-240, 10 Center Drive, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 3317 EP - 3324 VL - 49 IS - 8 SN - 0066-4804, 0066-4804 KW - Antifungal Agents KW - 0 KW - Echinocandins KW - Lipopeptides KW - Lipoproteins KW - Peptides, Cyclic KW - micafungin KW - R10H71BSWG KW - Index Medicus KW - Dose-Response Relationship, Drug KW - Humans KW - Drug Resistance, Fungal KW - Treatment Outcome KW - Child KW - Adolescent KW - Male KW - Female KW - Child, Preschool KW - Lipoproteins -- pharmacokinetics KW - Fever -- etiology KW - Peptides, Cyclic -- administration & dosage KW - Lipoproteins -- administration & dosage KW - Antifungal Agents -- pharmacokinetics KW - Antifungal Agents -- adverse effects KW - Peptides, Cyclic -- adverse effects KW - Mycoses -- prevention & control KW - Neutropenia -- complications KW - Lipoproteins -- adverse effects KW - Mycoses -- drug therapy KW - Antifungal Agents -- administration & dosage KW - Peptides, Cyclic -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68081060?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+agents+and+chemotherapy&rft.atitle=Safety%2C+tolerability%2C+and+pharmacokinetics+of+Micafungin+%28FK463%29+in+febrile+neutropenic+pediatric+patients.&rft.au=Seibel%2C+Nita+L%3BSchwartz%2C+Cindy%3BArrieta%2C+Antonio%3BFlynn%2C+Patricia%3BShad%2C+Aziza%3BAlbano%2C+Edith%3BKeirns%2C+James%3BLau%2C+Wendi+M%3BFacklam%2C+David+P%3BBuell%2C+Donald+N%3BWalsh%2C+Thomas+J&rft.aulast=Seibel&rft.aufirst=Nita&rft.date=2005-08-01&rft.volume=49&rft.issue=8&rft.spage=3317&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+agents+and+chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mycoses. 1999;42(7-8):431-42 [10546484] Clin Infect Dis. 2004 Nov 15;39(10):1407-16 [15546073] Antimicrob Agents Chemother. 2000 Mar;44(3):614-8 [10681327] Antimicrob Agents Chemother. 2000 Mar;44(3):619-21 [10681328] J Antimicrob Chemother. 2000 Sep;46(3):485-7 [10980180] Bone Marrow Transplant. 2000 Nov;26(9):999-1004 [11100280] Antimicrob Agents Chemother. 2001 Dec;45(12):3322-7 [11709303] J Antibiot (Tokyo). 2002 Feb;55(2):219-22 [12003006] Antimicrob Agents Chemother. 2002 Jun;46(6):1857-69 [12019101] Cancer. 2002 Oct 15;95(8):1767-72 [12365026] Cancer. 2003 May 1;97(9):2229-35 [12712476] Lancet. 2003 Oct 4;362(9390):1142-51 [14550704] Antimicrob Agents Chemother. 2004 Jun;48(6):2166-72 [15155217] Leuk Lymphoma. 2004 Apr;45(4):669-80 [15160938] Aliment Pharmacol Ther. 2004 Aug 15;20(4):475-81 [15298643] Am J Med. 1987 Dec;83(6):1103-10 [3332568] J Clin Oncol. 1990 Feb;8(2):280-6 [2299371] J Pediatr. 1992 Jun;120(6):987-93 [1593362] Bone Marrow Transplant. 1996 Dec;18 Suppl 3:S15-20 [8971401] Med Pediatr Oncol. 1998 Apr;30(4):233-9 [9473758] Clin Infect Dis. 1999 Nov;29(5):1210-9 [10524965] Antimicrob Agents Chemother. 2000 Jan;44(1):57-62 [10602723] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Increased bioaccumulation of urethane in CYP2E1-/- versus CYP2E1+/+ mice. AN - 68080461; 15879495 AB - Urethane is a fermentation by-product and a potent animal carcinogen. Human exposure to urethane occurs through consumption of alcoholic beverages and fermented foods. Recently, CYP2E1 was identified as the primary enzyme responsible for the metabolism of [(14)C]carbonyl-labeled urethane. Subsequently, attenuation of urethane-induced cell proliferation and genotoxicity in CYP2E1-/- mice was reported. The present work compares the metabolism of single versus multiple exposures of CYP2E1-/- and CYP2E1+/+ mice to (14)C-ethyl-labeled urethane. Urethane was administered as a single 10 or 100 mg/kg gavage dose or at 100 mg/kg/day for 5 consecutive days. CYP2E1+/+ mice administered single or multiple doses exhaled 78 to 88% of dose as (14)CO(2)/day. CYP2E1-/- mice eliminated 30 to 38% of a single dose as (14)CO(2) in 24 h and plateaued after day 3 at approximately 52% of dose/day. The concentrations of urethane-derived radioactivity in plasma and tissues were dose-dependent, increased as a function of the number of doses administered, and were significantly higher in CYP2E1-/- versus CYP2E1+/+ mice. Whereas urethane was the main chemical found in the plasma and tissues of CYP2E1-/- mice, it was not detectable in CYP2E1+/+ mice. In conclusion, multiple dosing led to considerable bioaccumulation of urethane in mice of both genotypes; however, greater retention occurred in CYP2E1-/- versus CYP2E1+/+ mice. Furthermore, greater bioaccumulation of (14)C-ethyl-labeled than [(14)C]carbonyl-labeled urethane was observed in mice. Comparison of the metabolism of ethyl-versus carbonyl-labeled urethane was necessary for tracing the source of CO(2) and led us to propose for the first time that C-hydroxylation is a likely pathway of urethane metabolism. JF - Drug metabolism and disposition: the biological fate of chemicals AU - Hoffler, Undi AU - Ghanayem, Burhan I AD - Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1144 EP - 1150 VL - 33 IS - 8 SN - 0090-9556, 0090-9556 KW - Carbon Radioisotopes KW - 0 KW - Carcinogens KW - Mutagens KW - Carbon Dioxide KW - 142M471B3J KW - Urethane KW - 3IN71E75Z5 KW - Cytochrome P-450 CYP2E1 KW - EC 1.14.13.- KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Carcinogens -- toxicity KW - Mutagens -- toxicity KW - Mice KW - Tissue Distribution KW - Diet KW - Carbon Dioxide -- metabolism KW - Male KW - Mice, Knockout KW - Urethane -- pharmacokinetics KW - Cytochrome P-450 CYP2E1 -- deficiency KW - Urethane -- metabolism KW - Cytochrome P-450 CYP2E1 -- metabolism KW - Urethane -- toxicity KW - Cytochrome P-450 CYP2E1 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68080461?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.atitle=Increased+bioaccumulation+of+urethane+in+CYP2E1-%2F-+versus+CYP2E1%2B%2F%2B+mice.&rft.au=Hoffler%2C+Undi%3BGhanayem%2C+Burhan+I&rft.aulast=Hoffler&rft.aufirst=Undi&rft.date=2005-08-01&rft.volume=33&rft.issue=8&rft.spage=1144&rft.isbn=&rft.btitle=&rft.title=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-08-04 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - India: alcohol and public health. AN - 68073898; 16042631 JF - Addiction (Abingdon, England) AU - Benegal, Vivek AD - Deaddiction Centre, National Institute of Mental Health and Neurosciences, Bangalore 560029, India. vbenegal@nimhans.kar.nic.in Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1051 EP - 1056 VL - 100 IS - 8 SN - 0965-2140, 0965-2140 KW - Index Medicus KW - India -- epidemiology KW - Social Change KW - Humans KW - Public Policy KW - Sex Distribution KW - Male KW - Female KW - Prevalence KW - Culture KW - Alcohol Drinking -- trends KW - Alcohol-Related Disorders -- prevention & control KW - Alcohol Drinking -- epidemiology KW - Alcohol-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68073898?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=India%3A+alcohol+and+public+health.&rft.au=Benegal%2C+Vivek&rft.aulast=Benegal&rft.aufirst=Vivek&rft.date=2005-08-01&rft.volume=100&rft.issue=8&rft.spage=1051&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-13 N1 - Date created - 2005-07-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. AN - 68073012; 16043845 AB - Pigment epithelium-derived factor (PEDF), a protein secreted by the retinal pigment epithelium (RPE), acts on retinal survival and angiogenesis. Because hypoxia and VEGF regulate matrix metalloproteinases (MMPs), their effects on PEDF proteolysis were explored. Mouse models for retinopathy of prematurity (ROP) were used. Cultured monkey RPE cells were exposed to low oxygen and chemical hypoxia mimetics. PEDF and VEGF mRNA levels in RPE were determined by RT-PCR. MMPs were assessed by zymography, DQ-gelatin degradation solution assays, and MMP immunostaining. PEDF proteolysis was assayed in solution and followed by SDS-PAGE and immunostaining. MMP induction by VEGF was performed in baby hamster kidney (BHK) cells. Retinal R28 cell survival, ex vivo chick embryonic aortic vessel sprouting, and directed in vivo angiogenesis assays were performed. Levels of PEDF in RPE/choroid significantly decreased in the ROP model. Hypoxia decreased PEDF levels in the media conditioned by RPE cells, with no significant change in PEDF mRNA. Conversely, PEDF proteolysis, gelatinolytic activities of approximately 57-kDa and approximately 86-kDa zymogens, and MMP-2 immunoreactivities increased with hypoxia. Addition of VEGF to BHK cells caused a time and dose-related upregulation of approximately 57-kDa zymogens and of DQ-gelatinolytic and PEDF-degrading activity. The PEDF-degrading activity and approximately 57-kDa zymogens in the BHK media shared MMP protease inhibition patterns and MMP-2 immunoreactivities with those in the vitreous. Limited proteolysis with MMP-2 and -9 degraded PEDF in a Ca(+2)-dependent fashion. MMP-mediated proteolysis of PEDF abolished the retinal survival and antiangiogenic activities of the PEDF protein. Hypoxia and VEGF can downregulate PEDF through proteolytic degradation. PEDF is a novel substrate for MMP-2 and -9. These results reveal a novel posttranslational mechanism for downregulating PEDF, and provide an explanation for hypoxia-provoked increases in VEGF/PEDF ratios, in angiogenesis and/or in neuronal death. JF - Investigative ophthalmology & visual science AU - Notari, Luigi AU - Miller, Amanda AU - Martínez, Alfredo AU - Amaral, Juan AU - Ju, Meihua AU - Robinson, Gregory AU - Smith, Lois E H AU - Becerra, S Patricia AD - Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-0607, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 2736 EP - 2747 VL - 46 IS - 8 SN - 0146-0404, 0146-0404 KW - Eye Proteins KW - 0 KW - Nerve Growth Factors KW - RNA, Messenger KW - Serpins KW - Vascular Endothelial Growth Factor A KW - pigment epithelium-derived factor KW - Matrix Metalloproteinase 2 KW - EC 3.4.24.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Animals KW - Oxygen -- toxicity KW - Electrophoresis, Polyacrylamide Gel KW - Pigment Epithelium of Eye -- metabolism KW - Chick Embryo KW - Humans KW - Macaca KW - Neovascularization, Pathologic -- metabolism KW - Infant, Newborn KW - Disease Models, Animal KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - Animals, Newborn KW - Blotting, Western KW - RNA, Messenger -- metabolism KW - Down-Regulation KW - Pigment Epithelium of Eye -- drug effects KW - Cells, Cultured KW - Mice, Inbred C57BL KW - Pigment Epithelium of Eye -- cytology KW - Vascular Endothelial Growth Factor A -- genetics KW - Vascular Endothelial Growth Factor A -- metabolism KW - Cricetinae KW - Retinopathy of Prematurity -- enzymology KW - Nerve Growth Factors -- metabolism KW - Matrix Metalloproteinase 9 -- metabolism KW - Nerve Growth Factors -- genetics KW - Eye Proteins -- metabolism KW - Eye Proteins -- genetics KW - Serpins -- metabolism KW - Serpins -- genetics KW - Matrix Metalloproteinase 2 -- metabolism KW - Hypoxia -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68073012?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Investigative+ophthalmology+%26+visual+science&rft.atitle=Pigment+epithelium-derived+factor+is+a+substrate+for+matrix+metalloproteinase+type+2+and+type+9%3A+implications+for+downregulation+in+hypoxia.&rft.au=Notari%2C+Luigi%3BMiller%2C+Amanda%3BMart%C3%ADnez%2C+Alfredo%3BAmaral%2C+Juan%3BJu%2C+Meihua%3BRobinson%2C+Gregory%3BSmith%2C+Lois+E+H%3BBecerra%2C+S+Patricia&rft.aulast=Notari&rft.aufirst=Luigi&rft.date=2005-08-01&rft.volume=46&rft.issue=8&rft.spage=2736&rft.isbn=&rft.btitle=&rft.title=Investigative+ophthalmology+%26+visual+science&rft.issn=01460404&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neoadjuvant docetaxel followed by adjuvant doxorubicin and cyclophosphamide in patients with stage III breast cancer. AN - 68066208; 15905305 AB - To evaluate clinical and pathologic response to neoadjuvant docetaxel therapy in patients with stage III breast cancer. Forty-five patients were planned to receive four cycles of docetaxel 100 mg/m2 every 3 weeks, followed by surgery, four cycles of doxorubicin 60 mg/m2 and cyclophosphamide 600 mg/m2 (AC) every 3 weeks, radiation therapy (RT), and tamoxifen when indicated. After four cycles of neoadjuvant docetaxel, the clinical response rate within the breast was 59% (95% CI 42% to 73%) and overall (breast and axilla) was 49% (95% CI 38% to 72%) in the intention-to-treat (ITT) population. At the time of surgery, 10% (n=4) of patients had a pathologic complete response (pCR) in the breast, 27% (n=11) had a pCR within the axillary lymph nodes, and 7% (n=3) had a pCR in the breast and axilla (95% CI 2% to 21%). An additional 5% (n=2) had minimal residual invasive tumor (<5 mm). The 5-year overall survival rate was 80%. The percentage of patients with grade 3/4 neutropenia was similar during docetaxel (93%) and AC (86%), while a greater percentage of patients had febrile neutropenia during docetaxel treatment (27%) compared with AC treatment (7%). Neoadjuvant docetaxel followed by surgery, adjuvant AC, hormonal therapy where indicated, and RT is an active regimen for patients with stage III breast cancer. JF - Annals of oncology : official journal of the European Society for Medical Oncology AU - Gradishar, W J AU - Wedam, S B AU - Jahanzeb, M AU - Erban, J AU - Limentani, S A AU - Tsai, K-T AU - Olsen, S R AU - Swain, S M AD - Northwestern University Feinberg School of Medicine, Chicago, IL, USA. swains@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1297 EP - 1304 VL - 16 IS - 8 SN - 0923-7534, 0923-7534 KW - Taxoids KW - 0 KW - docetaxel KW - 15H5577CQD KW - Doxorubicin KW - 80168379AG KW - Cyclophosphamide KW - 8N3DW7272P KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Neoplasm Staging KW - Humans KW - Neoadjuvant Therapy KW - Aged KW - Doxorubicin -- administration & dosage KW - Neoplasm, Residual -- drug therapy KW - Taxoids -- administration & dosage KW - Survival Rate KW - Neoplasm Invasiveness -- pathology KW - Adult KW - Treatment Outcome KW - Middle Aged KW - Chemotherapy, Adjuvant KW - Female KW - Remission Induction KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- pathology KW - Breast Neoplasms -- surgery KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68066208?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Behavioral+Development&rft.atitle=A+comparative+study+of+child+temperament+and+parenting+in+Beijing%2C+China+and+the+western+United+States&rft.au=Porter%2C+Christian+L.%3BHart%2C+Craig+H.%3BYang%2C+Chongming%3BRobinson%2C+Clyde+C.%3BOlsen%2C+Susanne+Frost%3BZeng%2C+Qing%3BOlsen%2C+Joseph+A.%3BJin%2C+Shenghua&rft.aulast=Porter&rft.aufirst=Christian&rft.date=2005-11-01&rft.volume=29&rft.issue=6&rft.spage=541&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Behavioral+Development&rft.issn=01650254&rft_id=info:doi/10.1080%2F01650250500147402 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-09 N1 - Date created - 2005-07-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The efficacy and safety of pain management before and after implementation of hospital-wide pain management standards: is patient safety compromised by treatment based solely on numerical pain ratings? AN - 68063760; 16037164 AB - Inadequate analgesia in hospitalized patients prompted the Joint Commission on Accreditation of Healthcare Organizations in 2001 to introduce standards that require pain assessment and treatment. In response, many institutions implemented treatment guided by patient reports of pain intensity indexed with a numerical scale. Patient safety associated with treatment of pain guided by a numerical pain treatment algorithm (NPTA) has not been examined. We reviewed patient satisfaction with pain control and opioid-related adverse drug reactions before and after implementation of our NPTA. Patient satisfaction with pain management, measured on a 1-5 scale, significantly improved from 4.13 to 4.38 (P < 0.001) after implementation of an NPTA. The incidence of opioid over sedation adverse drug reactions per 100,000 inpatient hospital days increased from 11.0 pre-NPTA to 24.5 post-NPTA (P < 0.001). Of these patients, 94% had a documented decrease in their level of consciousness preceding the event. Although there was an improvement in patient satisfaction, we experienced a more than two-fold increase in the incidence of opioid over sedation adverse drug reactions in our hospital after the implementation of NPTA. Most adverse drug reactions were preceded by a documented decrease in the patient's level of consciousness, which emphasizes the importance of clinical assessment in managing pain. Although patient satisfaction with pain management has significantly improved since the adoption of pain management standards, adverse drug reactions have more than doubled. For the treatment of pain to be safe and effective, we must consider more than just a one-dimensional numerical assessment of pain. JF - Anesthesia and analgesia AU - Vila, Hector AU - Smith, Robert A AU - Augustyniak, Michael J AU - Nagi, Peter A AU - Soto, Roy G AU - Ross, Thomas W AU - Cantor, Alan B AU - Strickland, Jennifer M AU - Miguel, Rafael V AD - Department of Interdisciplinary Oncology, The H. Lee Moffitt Cancer Center and Research Institute, National Cancer Institute, Tampa, FL 33612-9497, USA. vilah@moffitt.usf.edu Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 474 EP - 80, table of contents VL - 101 IS - 2 SN - 0003-2999, 0003-2999 KW - Analgesics, Opioid KW - 0 KW - Hypnotics and Sedatives KW - Abridged Index Medicus KW - Index Medicus KW - Patient Satisfaction KW - Humans KW - Safety KW - Algorithms KW - Aged KW - Aged, 80 and over KW - Adult KW - Drug Overdose KW - Analgesics, Opioid -- therapeutic use KW - Middle Aged KW - Guidelines as Topic KW - Analgesics, Opioid -- adverse effects KW - Hypnotics and Sedatives -- adverse effects KW - Female KW - Male KW - Hospitals -- standards KW - Pain Management KW - Pain Measurement -- standards UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68063760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anesthesia+and+analgesia&rft.atitle=The+efficacy+and+safety+of+pain+management+before+and+after+implementation+of+hospital-wide+pain+management+standards%3A+is+patient+safety+compromised+by+treatment+based+solely+on+numerical+pain+ratings%3F&rft.au=Vila%2C+Hector%3BSmith%2C+Robert+A%3BAugustyniak%2C+Michael+J%3BNagi%2C+Peter+A%3BSoto%2C+Roy+G%3BRoss%2C+Thomas+W%3BCantor%2C+Alan+B%3BStrickland%2C+Jennifer+M%3BMiguel%2C+Rafael+V&rft.aulast=Vila&rft.aufirst=Hector&rft.date=2005-08-01&rft.volume=101&rft.issue=2&rft.spage=474&rft.isbn=&rft.btitle=&rft.title=Anesthesia+and+analgesia&rft.issn=00032999&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-17 N1 - Date created - 2005-07-22 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Anesth Analg. 2006 Apr;102(4):1288; author reply 1288-9 [16551941] Anesth Analg. 2006 May;102(5):1596; author reply 1596-7 [16632864] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selected DNA repair polymorphisms and gastric cancer in Poland. AN - 68057945; 15802298 AB - Impaired DNA repair capacity may adversely affect cancer risk, particularly in subjects exposed to DNA damaging carcinogens, as found in tobacco smoke, or among subjects deficient for protective factors, as found in fruits and vegetables. We studied tobacco use, fruit and vegetable intake, and common non-synonymous single nucleotide polymorphisms in four DNA repair genes in relation to gastric cancer risk, in a population-based, case-control study of 281 incident gastric cancer cases and 390 controls, in Warsaw, Poland. Multivariate logistic regression analysis was performed to calculate odds ratios (OR) and 95% confidence intervals (CI). Increased risks of gastric cancer were found for smokers (OR=3.1, CI=1.9-5.1 for pack-years>or=40 versus never smokers) and subjects with low fruit intake (OR=2.2, CI=1.3-3.6 for 1st versus 4th quartile); risk associated with vegetable intake was not statistically significant. Allele frequencies among the controls were consistent with those previously reported for the 5 polymorphisms studied: XRCC1-Arg399Gln, XPD-Lys751Gln, MGMT-Ile143Val, Leu84Phe, and XRCC3-Thr241Met. None of the studied polymorphisms were independently associated with gastric cancer risk. Smoking-associated risks, however, were greatest for carriers of the XRCC1-399 ArgArg genotype (Pinteraction=0.004). Risks associated with low intake of fruits or vegetables tended to be modified by selected polymorphisms in XRCC1, XPD and MGMT (Pinteraction=0.1-0.2). Risk modification was not found for the other repair polymorphisms. Selected DNA repair polymorphisms did not have independent effects on gastric cancer risk; however, they may modify smoking- and probably diet-related risks for this disease. These results need replication in larger epidemiological studies of gastric cancer. JF - Carcinogenesis AU - Huang, Wen-Yi AU - Chow, Wong-Ho AU - Rothman, Nat AU - Lissowska, Jolanta AU - Llaca, Victor AU - Yeager, Meredith AU - Zatonski, Witold AU - Hayes, Richard B AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. huangw@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1354 EP - 1359 VL - 26 IS - 8 SN - 0143-3334, 0143-3334 KW - Codon KW - 0 KW - Index Medicus KW - Vegetables KW - Polymorphism, Single Nucleotide KW - Codon -- genetics KW - Humans KW - Aged KW - Risk Assessment KW - Smoking KW - Poland KW - Adult KW - Middle Aged KW - Diet KW - Fruit KW - Female KW - Male KW - Life Style KW - DNA Repair -- genetics KW - Polymorphism, Genetic KW - DNA Damage KW - Stomach Neoplasms -- genetics KW - Stomach Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057945?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Selected+DNA+repair+polymorphisms+and+gastric+cancer+in+Poland.&rft.au=Huang%2C+Wen-Yi%3BChow%2C+Wong-Ho%3BRothman%2C+Nat%3BLissowska%2C+Jolanta%3BLlaca%2C+Victor%3BYeager%2C+Meredith%3BZatonski%2C+Witold%3BHayes%2C+Richard+B&rft.aulast=Huang&rft.aufirst=Wen-Yi&rft.date=2005-08-01&rft.volume=26&rft.issue=8&rft.spage=1354&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - B-cell recovery following rituximab-based therapy is associated with perturbations in stromal derived factor-1 and granulocyte homeostasis. AN - 68057326; 15718416 AB - The occurrence of delayed neutropenia following rituximab is poorly defined and of unknown cause. We hypothesized it may be related to perturbations of stromal derived factor-1 (SDF-1) and granulocyte homeostasis. Late-onset neutropenia (LON) was investigated in 130 patients with untreated aggressive B-cell lymphoma receiving DA-EPOCH (dose-adjusted etoposide, prednisone, Oncovin [vincristine], cyclophosphamide, and hydroxydaunorubicin) chemotherapy with or without rituximab. All patients were in remission and had no known causes for neutropenia. LON occurred in 6 (8%) of 76 patients receiving rituximab and 0 of 54 patients not receiving rituximab (P = .04). The median onset was 175 days (range, 77-204 days) after treatment with a median duration of 14 days (range, 11-16 days). In a subset of 24 patients, a significant correlation was found between rapid B-cell recovery and granulocyte decline over the 6-month recovery period (R = -0.53; P = .04). Rapid B-cell recovery directly correlated with prerecovery SDF-1 levels (R = 0.65; P = .015) and SDF-1 decline (R = -0.67; P = .013) after recovery. Our results suggest that early B-cell lymphopoiesis is important for B-cell recovery following rituximab, and that perturbation of SDF-1 during B-cell recovery retards neutrophil egress from the bone marrow. These findings illustrate the dual role of SDF-1 in human B-cell and granulocyte homeostasis. JF - Blood AU - Dunleavy, Kieron AU - Hakim, Frances AU - Kim, Hyun Kyung AU - Janik, John E AU - Grant, Nicole AU - Nakayama, Takayuki AU - White, Therese AU - Wright, George AU - Kwak, Larry AU - Gress, Ronald AU - Tosato, Giovanna AU - Wilson, Wyndham H AD - Experimental Transplantation and Immunology Branch, CCR, NCI, Bldg 10, Rm 12-N-226, Bethesda, MD, 20892-1868, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 795 EP - 802 VL - 106 IS - 3 SN - 0006-4971, 0006-4971 KW - Antibodies, Monoclonal KW - 0 KW - Antibodies, Monoclonal, Murine-Derived KW - CXCL12 protein, human KW - Chemokine CXCL12 KW - Chemokines, CXC KW - Rituximab KW - 4F4X42SYQ6 KW - Abridged Index Medicus KW - Index Medicus KW - Lymphopoiesis KW - Neutropenia -- etiology KW - Humans KW - Retrospective Studies KW - Neutropenia -- chemically induced KW - Aged KW - Drug Evaluation KW - Lymphoma, B-Cell -- complications KW - Lymphoma, B-Cell -- drug therapy KW - Kinetics KW - Regeneration KW - Adult KW - Middle Aged KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Male KW - Female KW - Chemokines, CXC -- physiology KW - Granulocytes -- physiology KW - Antibodies, Monoclonal -- adverse effects KW - Homeostasis KW - B-Lymphocytes -- physiology KW - Chemokines, CXC -- blood KW - Antibodies, Monoclonal -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057326?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=B-cell+recovery+following+rituximab-based+therapy+is+associated+with+perturbations+in+stromal+derived+factor-1+and+granulocyte+homeostasis.&rft.au=Dunleavy%2C+Kieron%3BHakim%2C+Frances%3BKim%2C+Hyun+Kyung%3BJanik%2C+John+E%3BGrant%2C+Nicole%3BNakayama%2C+Takayuki%3BWhite%2C+Therese%3BWright%2C+George%3BKwak%2C+Larry%3BGress%2C+Ronald%3BTosato%2C+Giovanna%3BWilson%2C+Wyndham+H&rft.aulast=Dunleavy&rft.aufirst=Kieron&rft.date=2005-08-01&rft.volume=106&rft.issue=3&rft.spage=795&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-21 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Clin Oncol. 1999 Jun;17(6):1851-7 [10561225] Immunity. 1999 Apr;10(4):463-71 [10229189] Cancer Res. 2001 Jul 1;61(13):5028-37 [11431337] Immunity. 2001 Aug;15(2):323-34 [11520466] N Engl J Med. 2002 Jan 24;346(4):235-42 [11807147] Blood. 2002 Apr 15;99(8):2685-93 [11929754] Blood. 2002 Apr 15;99(8):2703-11 [11929756] Nat Immunol. 2002 Jul;3(7):687-94 [12068293] Exp Hematol. 2002 Sep;30(9):973-81 [12225788] J Clin Invest. 2003 Jan;111(2):187-96 [12531874] Blood. 2003 Jun 15;101(12):4653-9 [12609827] Br J Haematol. 2003 Jun;121(6):913-8 [12786803] N Engl J Med. 2003 Jun 26;348(26):2691-4; discussion 2691-4 [12826650] Immunity. 2003 Oct;19(4):583-93 [14563322] J Leukoc Biol. 2003 Nov;74(5):880-8 [12960279] Bone Marrow Transplant. 2004 May;33(9):921-3 [15034544] Immunity. 2004 Jun;20(6):707-18 [15189736] Blood. 2004 Jul 15;104(2):565-71 [15054039] Nat Med. 2004 Aug;10(8):858-64 [15235597] J Immunol. 1980 Oct;125(4):1678-85 [6157744] J Exp Med. 1981 Sep 1;154(3):737-49 [7024458] Eur J Immunol. 1986 Aug;16(8):881-7 [3091375] Blood. 1994 Jan 15;83(2):435-45 [7506951] Immunol Today. 1994 Sep;15(9):450-4 [7524522] J Clin Oncol. 1997 Oct;15(10):3266-74 [9336364] Blood. 1997 Nov 1;90(9):3789-98 [9345067] J Immunol. 1998 Feb 1;160(3):1522-31 [9570576] Nature. 1998 Jun 11;393(6685):595-9 [9634238] J Clin Oncol. 2000 Sep;18(17):3135-43 [10963642] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - MnSOD inhibits proline oxidase-induced apoptosis in colorectal cancer cells. AN - 68056785; 15817612 AB - Proline oxidase (POX), localized on inner mitochondrial membranes, is encoded by a p53-induced gene and metabolically participates in p53-induced apoptosis. Previously, we showed that POX catalyzed the generation of reactive oxygen species (ROS). We and others have demonstrated that overexpression of POX, independent of p53, causes apoptotic cell death in a variety of cancer cells. But a necessary role for ROS remains uncertain. Therefore, we asked whether superoxide dismutases (SOD) and catalase (CAT), important antioxidant enzymes, might interfere with the POX-dependent induction of apoptosis. In this study, we used DLD-1 colorectal cancer cells stably transfected with the POX gene under the control of a tetracycline-inducible promoter. When doxycycline was removed from the culture medium and the expression of POX was induced, apoptotic cell death was initiated. To examine the importance of the ROS-dependent component of the pathway, we infected DLD-1 POX cells with recombinant adenoviruses containing MnSOD, CuZnSOD, CAT or varying combinations of these adenoviruses followed by induced expression of POX. The expression of MnSOD inhibited POX-induced apoptosis, but others did not. Mechanistically, mitochondria-localized MnSOD dramatically reduced the release of cytochrome c to cytosol by POX. Compared with control cells, MnSOD-expressing DLD-1 POX cells generated a higher concentration of H2O2 owing to dismutation of superoxide radicals, which was elevated by POX. Thus, these data further suggest that the generation of superoxide radicals plays a crucial role in POX-induced apoptosis and the process is partially blocked by MnSOD. JF - Carcinogenesis AU - Liu, Yongmin AU - Borchert, Gregory L AU - Donald, Steven P AU - Surazynski, Arkadiusz AU - Hu, Chien-An AU - Weydert, Christine J AU - Oberley, Larry W AU - Phang, James M AD - Metabolism and Cancer Susceptibility Section, Laboratory for Comparative Carcinogenesis, National Cancer Institute, Frederick, MD 21702, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1335 EP - 1342 VL - 26 IS - 8 SN - 0143-3334, 0143-3334 KW - Reactive Oxygen Species KW - 0 KW - Recombinant Proteins KW - Tumor Suppressor Protein p53 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Catalase KW - EC 1.11.1.6 KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Proline Oxidase KW - EC 1.5.3.- KW - Acetylcysteine KW - WYQ7N0BPYC KW - Index Medicus KW - Catalase -- metabolism KW - Apoptosis KW - Transfection KW - Recombinant Proteins -- metabolism KW - Kinetics KW - Hydrogen Peroxide -- metabolism KW - Humans KW - Cell Line, Tumor KW - Acetylcysteine -- pharmacology KW - Cell Death -- drug effects KW - Colorectal Neoplasms KW - Proline Oxidase -- metabolism KW - Superoxide Dismutase -- metabolism KW - Tumor Suppressor Protein p53 -- genetics KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056785?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.genre=dissertations+%26+theses&rft.jtitle=&rft.atitle=&rft.au=Wei%2C+Shih-Tai&rft.aulast=Wei&rft.aufirst=Shih-Tai&rft.date=1998-02-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Perceived+parenting+patterns+and+adolescents%27+coping+styles+in+Chinese+culture&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparing measures of acute bowel toxicity in patients with prostate cancer treated with external beam radiation therapy. AN - 68056379; 16029787 AB - This study strives to compare early measures of bowel toxicity in patients with prostate cancer receiving definitive or adjuvant 3D conformal external beam radiation therapy and concurrent daily endorectal application of amifostine. Eighteen patients were enrolled in the clinical study with a median follow-up of 12 months. Prescription doses ranged from 66 Gy to 76 Gy with a daily fractionation of 2 Gy. Acute bowel toxicity was measured at baseline, at Weeks 5 and 7 of radiotherapy, and at 1 and 3 months after the completion of therapy. Measures of acute bowel toxicity included the Radiation Therapy Oncology Group (RTOG) acute radiation morbidity scoring criteria, Expanded Prostate Cancer Index Composite (EPIC) self-assessment questionnaires, and proctoscopic examinations. The mean EPIC bowel scores changed significantly through the course of therapy and follow-up (p < 0.0001), with a progressive decrease in scores at Weeks 5 and 7 of treatment, a partial recovery at 3 months, and a correlation to the gold standard RTOG grade (p = 0.004). Proctoscopic toxicity scores were low, did not vary over time, and did not correlate with either EPIC or RTOG scores. The EPIC questionnaire measurements are most sensitive to changes in acute bowel toxicity through a course of radiotherapy and correlate with RTOG acute toxicity scores. Endoscopic examination of the rectal mucosa at the end and immediate follow-up of a course of therapy does not seem to be informative or reproducible between observers in the acute setting. JF - International journal of radiation oncology, biology, physics AU - Muanza, Thierry M AU - Albert, Paul S AU - Smith, Sharon AU - Godette, Denise AU - Crouse, Nancy Sears AU - Cooley-Zgela, Theresa AU - Sciuto, Linda AU - Camphausen, Kevin AU - Coleman, C Norman AU - Ménard, Cynthia AD - Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1316 EP - 1321 VL - 62 IS - 5 SN - 0360-3016, 0360-3016 KW - Radiation-Protective Agents KW - 0 KW - Amifostine KW - M487QF2F4V KW - Index Medicus KW - Radiotherapy, Conformal KW - Proctoscopy KW - Radiotherapy Dosage KW - Humans KW - Chi-Square Distribution KW - Surveys and Questionnaires KW - Quality of Life KW - Aged KW - Middle Aged KW - Statistics, Nonparametric KW - Male KW - Amifostine -- therapeutic use KW - Radiation-Protective Agents -- therapeutic use KW - Rectum -- radiation effects KW - Radiation Injuries -- pathology KW - Prostatic Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056379?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.atitle=Comparing+measures+of+acute+bowel+toxicity+in+patients+with+prostate+cancer+treated+with+external+beam+radiation+therapy.&rft.au=Muanza%2C+Thierry+M%3BAlbert%2C+Paul+S%3BSmith%2C+Sharon%3BGodette%2C+Denise%3BCrouse%2C+Nancy+Sears%3BCooley-Zgela%2C+Theresa%3BSciuto%2C+Linda%3BCamphausen%2C+Kevin%3BColeman%2C+C+Norman%3BM%C3%A9nard%2C+Cynthia&rft.aulast=Muanza&rft.aufirst=Thierry&rft.date=2005-08-01&rft.volume=62&rft.issue=5&rft.spage=1316&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.issn=03603016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selective depletion of alloreactive donor lymphocytes: a novel method to reduce the severity of graft-versus-host disease in older patients undergoing matched sibling donor stem cell transplantation. AN - 68056332; 15817673 AB - We have selectively depleted host-reactive donor T cells from peripheral blood stem cell (PBSC) transplant allografts ex vivo using an anti-CD25 immunotoxin. We report a clinical trial to decrease graft-versus-host disease (GVHD) in elderly patients receiving selectively depleted PBSC transplants from HLA-identical sibling donors. Sixteen patients (median age, 65 years [range, 51-73 years]), with advanced hematologic malignancies underwent transplantation following reduced-intensity conditioning with fludarabine and either cyclophosphamide (n = 5), melphalan (n = 5), or busulfan (n = 6). Cyclosporine was used as sole GVHD prophylaxis. The allograft contained a median of 4.5 x 10(6) CD34 cells/kg (range, 3.4-7.3 x 10(6) CD34 cells/kg) and 1.0 x 10(8)/kg (range, 0.2-1.5 x 10(8)/kg) selectively depleted T cells. Fifteen patients achieved sustained engraftment. The helper T-lymphocyte precursor (HTLp) frequency assay demonstrated successful (mean, 5-fold) depletion of host-reactive donor T cells, with conservation of third-party response in 9 of 11 cases tested. Actuarial rates of acute GVHD were 46% +/- 13% for grades II to IV and 12% +/- 8% for grades III to IV. These results suggest that allodepletion of donor cells ex vivo is clinically feasible in older patients and may reduce the rate of severe acute GVHD. Further studies with selectively depleted transplants to evaluate graft-versus-leukemia (GVL) and survival are warranted. JF - Blood AU - Solomon, Scott R AU - Mielke, Stephan AU - Savani, Bipin N AU - Montero, Aldemar AU - Wisch, Laura AU - Childs, Richard AU - Hensel, Nancy AU - Schindler, John AU - Ghetie, Victor AU - Leitman, Susan F AU - Mai, Thao AU - Carter, Charles S AU - Kurlander, Roger AU - Read, Elizabeth J AU - Vitetta, Ellen S AU - Barrett, A John AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, NHLBI, NIH Bldg 10, Hatfield CRC, Rm 3-5320, 10 Center Dr, MSC 1202, Bethesda, MD 20892-1202, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1123 EP - 1129 VL - 106 IS - 3 SN - 0006-4971, 0006-4971 KW - Abridged Index Medicus KW - Index Medicus KW - Hematologic Neoplasms -- therapy KW - Hematologic Neoplasms -- complications KW - Humans KW - Histocompatibility KW - Graft Survival KW - Transplantation Chimera KW - Incidence KW - Aged KW - Siblings KW - Middle Aged KW - Transplantation, Homologous KW - Recurrence KW - Male KW - Female KW - Lymphocyte Depletion -- methods KW - Peripheral Blood Stem Cell Transplantation -- adverse effects KW - Peripheral Blood Stem Cell Transplantation -- methods KW - Graft vs Host Disease -- prevention & control KW - Cell Separation -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056332?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Selective+depletion+of+alloreactive+donor+lymphocytes%3A+a+novel+method+to+reduce+the+severity+of+graft-versus-host+disease+in+older+patients+undergoing+matched+sibling+donor+stem+cell+transplantation.&rft.au=Solomon%2C+Scott+R%3BMielke%2C+Stephan%3BSavani%2C+Bipin+N%3BMontero%2C+Aldemar%3BWisch%2C+Laura%3BChilds%2C+Richard%3BHensel%2C+Nancy%3BSchindler%2C+John%3BGhetie%2C+Victor%3BLeitman%2C+Susan+F%3BMai%2C+Thao%3BCarter%2C+Charles+S%3BKurlander%2C+Roger%3BRead%2C+Elizabeth+J%3BVitetta%2C+Ellen+S%3BBarrett%2C+A+John&rft.aulast=Solomon&rft.aufirst=Scott&rft.date=2005-08-01&rft.volume=106&rft.issue=3&rft.spage=1123&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-21 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Blood. 1990 Feb 1;75(3):555-62 [2297567] Biol Blood Marrow Transplant. 2004 Apr;10(4):259-68 [15077224] Blood. 1994 Jan 1;83(1):288-98 [8274744] Bone Marrow Transplant. 1994 Oct;14(4):517-24 [7858526] Blood. 1995 Aug 15;86(4):1261-8 [7632930] Blood. 1995 Sep 1;86(5):2041-50 [7655033] Bone Marrow Transplant. 1996 May;17(5):793-9 [8733700] Bone Marrow Transplant. 1996 Aug;18(2):415-20 [8864455] Transplantation. 1997 Sep 27;64(6):836-41 [9326407] Transplantation. 1997 Oct 27;64(8):1147-52 [9355832] Br J Haematol. 1998 Jun;101(3):565-70 [9633903] Transplantation. 1999 Jan 15;67(1):124-30 [9921808] Bone Marrow Transplant. 1999 Jan;23(2):137-44 [10197798] Br J Haematol. 1999 Apr;105(1):288-94 [10233396] Blood. 1999 May 15;93(10):3550-7 [10233908] Bone Marrow Transplant. 1999 May;23(10):1071-9 [10373075] Br J Haematol. 1999 Oct;107(1):169-75 [10520038] Blood. 2004 Dec 1;104(12):3429-36 [15284108] Cytotherapy. 2005;7(2):109-15 [16040390] Br J Haematol. 2000 Jun;109(3):644-51 [10886218] Leukemia. 2001 Feb;15(2):293-302 [11236950] Bone Marrow Transplant. 2001 May;27(9):949-58 [11436105] Immunol Rev. 2001 Aug;182:58-67 [11722623] Blood. 2002 Apr 15;99(8):3041-9 [11929798] Blood. 2002 May 1;99(9):3083-8 [11964269] Blood. 2002 Jul 15;100(2):375-82 [12091325] Lancet. 2002 Jul 13;360(9327):130-7 [12126823] J Exp Med. 2002 Aug 5;196(3):401-6 [12163568] Biol Blood Marrow Transplant. 2002;8(10):525-35 [12434947] Cytotherapy. 2002;4(5):395-406 [12473206] Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1180-4 [12531922] Blood. 2004 Feb 1;103(3):1158-65 [14525783] Bone Marrow Transplant. 2004 Feb;33(4):367-75 [14716351] Transplantation. 1990 Jul;50(1):1-7 [2142343] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. AN - 68054611; 16024799 AB - ING2 is a candidate tumor suppressor gene that can activate p53 by enhancing its acetylation. Here, we demonstrate that ING2 is also involved in p53-mediated replicative senescence. ING2 protein expression increased in late-passage human primary cells, and it colocalizes with serine 15-phosphorylated p53. ING2 and p53 also complexed with the histone acetyltransferase p300. ING2 enhanced the interaction between p53 and p300 and acted as a cofactor for p300-mediated p53 acetylation. The level of ING2 expression directly modulated the onset of replicative senescence. While overexpression of ING2 induced senescence in young fibroblasts in a p53-dependent manner, expression of ING2 small interfering RNA delayed the onset of senescence. Hence, ING2 can act as a cofactor of p300 for p53 acetylation and thereby plays a positive regulatory role during p53-mediated replicative senescence. JF - Molecular and cellular biology AU - Pedeux, Remy AU - Sengupta, Sagar AU - Shen, Jiang Cheng AU - Demidov, Oleg N AU - Saito, Shin'ichi AU - Onogi, Hitoshi AU - Kumamoto, Kensuke AU - Wincovitch, Stephen AU - Garfield, Susan H AU - McMenamin, Mary AU - Nagashima, Makoto AU - Grossman, Steven R AU - Appella, Ettore AU - Harris, Curtis C AD - Laboratory of Human Carcinogenesis, CCR, NCI, NIH, 37 Convent Dr., Bldg 37, Room 3068, Bethesda, MD 20892-4255, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 6639 EP - 6648 VL - 25 IS - 15 SN - 0270-7306, 0270-7306 KW - Homeodomain Proteins KW - 0 KW - ING2 protein, human KW - Nuclear Proteins KW - Receptors, Cytoplasmic and Nuclear KW - Trans-Activators KW - Tumor Suppressor Protein p53 KW - Tumor Suppressor Proteins KW - Serine KW - 452VLY9402 KW - Index Medicus KW - Acetylation KW - Phosphorylation KW - Humans KW - Protein Processing, Post-Translational KW - Cell Division -- physiology KW - Cell Proliferation KW - Serine -- metabolism KW - Cell Line KW - Tumor Suppressor Proteins -- biosynthesis KW - Receptors, Cytoplasmic and Nuclear -- physiology KW - Trans-Activators -- metabolism KW - Homeodomain Proteins -- biosynthesis KW - Tumor Suppressor Proteins -- physiology KW - Homeodomain Proteins -- physiology KW - Cell Aging -- physiology KW - Trans-Activators -- physiology KW - Receptors, Cytoplasmic and Nuclear -- biosynthesis KW - Nuclear Proteins -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism KW - Nuclear Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68054611?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biology&rft.atitle=ING2+regulates+the+onset+of+replicative+senescence+by+induction+of+p300-dependent+p53+acetylation.&rft.au=Pedeux%2C+Remy%3BSengupta%2C+Sagar%3BShen%2C+Jiang+Cheng%3BDemidov%2C+Oleg+N%3BSaito%2C+Shin%27ichi%3BOnogi%2C+Hitoshi%3BKumamoto%2C+Kensuke%3BWincovitch%2C+Stephen%3BGarfield%2C+Susan+H%3BMcMenamin%2C+Mary%3BNagashima%2C+Makoto%3BGrossman%2C+Steven+R%3BAppella%2C+Ettore%3BHarris%2C+Curtis+C&rft.aulast=Pedeux&rft.aufirst=Remy&rft.date=2005-08-01&rft.volume=25&rft.issue=15&rft.spage=6639&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biology&rft.issn=02707306&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Cancer. 2004 Apr 10;109(3):476-9 [14961591] Exp Cell Res. 1965 Mar;37:614-36 [14315085] Nature. 2004 Mar 18;428(6980):328-32 [15029197] Oncogene. 2004 Apr 12;23(16):2919-33 [15077154] Proc Natl Acad Sci U S A. 2004 May 11;101(19):7386-91 [15123817] Oncogene. 2004 May 13;23(22):4007-13 [15007388] Mol Cell. 2004 May 21;14(4):501-13 [15149599] Cancer Res. 2004 Jun 1;64(11):3748-52 [15172978] Mol Cell. 2004 Jul 2;15(1):83-94 [15225550] EMBO J. 2004 Jul 7;23(13):2554-63 [15192702] Cell Cycle. 2004 Apr;3(4):436-8 [14976431] Nature. 1990 May 31;345(6274):458-60 [2342578] Trends Biochem Sci. 1995 Feb;20(2):56-9 [7701562] Cancer Res. 1995 Jun 1;55(11):2404-9 [7538902] Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8348-52 [7667293] Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9363-7 [7568133] J Virol. 1996 Aug;70(8):5701-5 [8764092] Oncogene. 1996 Nov 21;13(10):2097-104 [8950976] Cell. 1997 Mar 7;88(5):593-602 [9054499] Mol Cell Biol. 1997 Apr;17(4):2014-9 [9121449] Cell. 1997 Aug 22;90(4):595-606 [9288740] EMBO J. 1997 Oct 1;16(19):6018-33 [9312059] Nature. 1998 Jan 15;391(6664):295-8 [9440695] EMBO J. 1999 Dec 15;18(24):7002-10 [10601022] Mol Cell Biol. 2000 Apr;20(8):2803-8 [10733583] Nature. 2000 Jul 13;406(6792):207-10 [10910364] Genes Dev. 2000 Aug 15;14(16):2015-27 [10950866] Eur J Biochem. 2001 May;268(10):2764-72 [11358490] Eur J Biochem. 2001 May;268(10):2773-8 [11358491] Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9671-6 [11481424] Mol Cell Biol. 2001 Nov;21(22):7629-40 [11604499] Cell. 2001 Dec 28;107(7):815-8 [11779456] Science. 1998 Jan 16;279(5349):349-52 [9454332] Mol Cell Biol. 1998 Mar;18(3):1611-21 [9488478] J Biol Chem. 1998 Dec 4;273(49):33048-53 [9830059] Mol Cell Biol. 1999 Apr;19(4):3103-14 [10082577] Science. 2002 Apr 19;296(5567):550-3 [11910072] J Biol Chem. 2002 Aug 16;277(33):29832-9 [12015309] Trends Cell Biol. 2002 Nov;12(11):532-8 [12446115] Oncogene. 2003 Jan 23;22(3):343-50 [12545155] EMBO J. 2003 Mar 3;22(5):1210-22 [12606585] Curr Opin Cell Biol. 2003 Apr;15(2):164-71 [12648672] Science. 2003 Apr 11;300(5617):342-4 [12690203] Cancer Res. 2003 May 15;63(10):2373-8 [12750254] Cell. 2003 Jun 13;113(6):703-16 [12809602] J Clin Pathol. 2003 Jul;56(7):491-6 [12835293] Cell. 2003 Jul 11;114(1):99-111 [12859901] EMBO J. 2003 Aug 15;22(16):4212-22 [12912919] Curr Biol. 2003 Sep 2;13(17):1549-56 [12956959] Cancer Res. 2003 Sep 15;63(18):5785-92 [14522900] Cancer Cell. 2003 Oct;4(4):311-9 [14585358] Nature. 2003 Nov 13;426(6963):194-8 [14608368] Nat Cell Biol. 2004 Feb;6(2):168-70 [14755273] Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2259-64 [14982997] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functional consequences of single nucleotide polymorphisms in the human organic anion transporter hOAT1 (SLC22A6). AN - 68046678; 15914676 AB - The human organic anion transporter hOAT1 (SLC22A6) contributes to the uptake of a range of small organic anions across the basolateral membrane of the renal proximal tubule and drives their urinary elimination. The aim of this study was to identify genetic variants of hOAT1 and to investigate potential effects on the functional properties of this transporter. Twenty single nucleotide polymorphisms (SNPs) in hOAT1 were identified in genomic DNA from 92 individuals of African, Asian, and Caucasian origin. Two SNPs encoded changes in amino acid sequence; arginine to histidine (residue 50) and lysine to isoleucine (residue 525). Significantly, these SNPs were only present in the samples of African origin. When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). Kinetic analysis indicated that the transport affinity (K(m)) for PAH was unchanged in the variants, compared with wild type. Interestingly, the K(m) for the nucleoside phosphonate analogs adefovir, cidofovir, and tenofovir seemed to be decreased in the R50H-hOAT1 variant compared with the wild type, whereas the kinetics of K525I-hOAT1 remained unchanged. In conclusion, this is the first study to identify variation of hOAT1 in a racially diverse sample and to investigate the functional properties of the resulting variants. Since hOAT1 has been suggested as the basis of nephrotoxicity induced by nucleoside phosphonate analogs, this study raises the intriguing possibility that individuals with genetic variation in hOAT1, such as R50H, may display different handling of these drugs. JF - The Journal of pharmacology and experimental therapeutics AU - Bleasby, Kelly AU - Hall, Laura A AU - Perry, Jennifer L AU - Mohrenweiser, Harvey W AU - Pritchard, John B AD - Laboratory of Pharmacology and Chemistry, The National Institute of Environmental Health Sciences, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 923 EP - 931 VL - 314 IS - 2 SN - 0022-3565, 0022-3565 KW - Antiviral Agents KW - 0 KW - Organic Anion Transport Protein 1 KW - Organophosphonates KW - RNA, Complementary KW - Recombinant Proteins KW - adefovir KW - 6GQP90I798 KW - Cytosine KW - 8J337D1HZY KW - DNA KW - 9007-49-2 KW - Tenofovir KW - 99YXE507IL KW - Adenine KW - JAC85A2161 KW - cidofovir KW - JIL713Q00N KW - p-Aminohippuric Acid KW - Y79XT83BJ9 KW - Index Medicus KW - Animals KW - Genetic Variation KW - Organophosphonates -- metabolism KW - Models, Molecular KW - Mutagenesis, Site-Directed -- genetics KW - Humans KW - Amino Acid Sequence KW - Reverse Transcriptase Polymerase Chain Reaction KW - Recombinant Proteins -- genetics KW - Antiviral Agents -- metabolism KW - Cytosine -- analogs & derivatives KW - RNA, Complementary -- genetics KW - Cytosine -- metabolism KW - Xenopus laevis KW - Adenine -- metabolism KW - Oocytes -- metabolism KW - Recombinant Proteins -- metabolism KW - DNA -- genetics KW - RNA, Complementary -- biosynthesis KW - Oocytes -- drug effects KW - Molecular Sequence Data KW - p-Aminohippuric Acid -- metabolism KW - Adenine -- analogs & derivatives KW - Organic Anion Transport Protein 1 -- genetics KW - Organic Anion Transport Protein 1 -- metabolism KW - Polymorphism, Single Nucleotide -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68046678?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Functional+consequences+of+single+nucleotide+polymorphisms+in+the+human+organic+anion+transporter+hOAT1+%28SLC22A6%29.&rft.au=Bleasby%2C+Kelly%3BHall%2C+Laura+A%3BPerry%2C+Jennifer+L%3BMohrenweiser%2C+Harvey+W%3BPritchard%2C+John+B&rft.aulast=Bleasby&rft.aufirst=Kelly&rft.date=2005-08-01&rft.volume=314&rft.issue=2&rft.spage=923&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparison of cannabidiol, antioxidants, and diuretics in reversing binge ethanol-induced neurotoxicity. AN - 68040315; 15878999 AB - Binge alcohol consumption in the rat induces substantial neurodegeneration in the hippocampus and entorhinal cortex. Oxidative stress and cytotoxic edema have both been shown to be involved in such neurotoxicity, whereas N-methyl-d-aspartate (NMDA) receptor activity has been implicated in alcohol withdrawal and excitoxic injury. Because the nonpsychoactive cannabinoid cannabidiol (CBD) was previously shown in vitro to prevent glutamate toxicity through its ability to reduce oxidative stress, we evaluated CBD as a neuroprotectant in a rat binge ethanol model. When administered concurrently with binge ethanol exposure, CBD protected against hippocampal and entorhinal cortical neurodegeneration in a dose-dependent manner. Similarly, the common antioxidants butylated hydroxytoluene and alpha-tocopherol also afforded significant protection. In contrast, the NMDA receptor antagonists dizocilpine (MK-801) and memantine did not prevent cell death. Of the diuretics tested, furosemide was protective, whereas the other two anion exchanger inhibitors, L-644,711 [(R)-(+)-(5,6-dichloro2,3,9,9a-tetrahydro 3-oxo-9a-propyl-1H-fluoren-7-yl)oxy acetic acid] and bumetanide, were ineffective. In vitro comparison of these diuretics indicated that furosemide is also a potent antioxidant, whereas the nonprotective diuretics are not. The lack of efficacy of L-644,711 and bumetanide suggests that the antioxidant rather than the diuretic properties of furosemide contribute most critically to its efficacy in reversing ethanol-induced neurotoxicity in vitro, in our model. This study provides the first demonstration of CBD as an in vivo neuroprotectant and shows the efficacy of lipophilic antioxidants in preventing binge ethanol-induced brain injury. JF - The Journal of pharmacology and experimental therapeutics AU - Hamelink, Carol AU - Hampson, Aidan AU - Wink, David A AU - Eiden, Lee E AU - Eskay, Robert L AD - Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulatiuon, National Institute of Mental Health/NIH, Bldg. 49, Room 5A-35, 9000 Rockville Pike, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 780 EP - 788 VL - 314 IS - 2 SN - 0022-3565, 0022-3565 KW - Antioxidants KW - 0 KW - Central Nervous System Depressants KW - Diuretics KW - Neuroprotective Agents KW - Cannabidiol KW - 19GBJ60SN5 KW - Ethanol KW - 3K9958V90M KW - Iron KW - E1UOL152H7 KW - Index Medicus KW - Rats KW - Oxidation-Reduction KW - Animals KW - Rats, Sprague-Dawley KW - Brain Chemistry -- drug effects KW - Male KW - Catalysis KW - Ethanol -- blood KW - Central Nervous System Depressants -- toxicity KW - Central Nervous System Depressants -- antagonists & inhibitors KW - Cannabidiol -- pharmacology KW - Antioxidants -- pharmacology KW - Central Nervous System Depressants -- blood KW - Ethanol -- antagonists & inhibitors KW - Diuretics -- pharmacology KW - Ethanol -- toxicity KW - Neurotoxicity Syndromes -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68040315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Comparison+of+cannabidiol%2C+antioxidants%2C+and+diuretics+in+reversing+binge+ethanol-induced+neurotoxicity.&rft.au=Hamelink%2C+Carol%3BHampson%2C+Aidan%3BWink%2C+David+A%3BEiden%2C+Lee+E%3BEskay%2C+Robert+L&rft.aulast=Hamelink&rft.aufirst=Carol&rft.date=2005-08-01&rft.volume=314&rft.issue=2&rft.spage=780&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevalence and correlates of tobacco use and nicotine dependence among psychiatric patients in India. AN - 68038980; 16022927 AB - Tobacco use among psychiatric patients in developing countries has not been well-investigated. To address this issue, we screened consecutive admissions to a major psychiatric hospital in southern India, and assessed the prevalence and correlates of tobacco use and nicotine dependence. Patients (n=988) provided information about their use of tobacco products, and participated in an interview that included the Fagerström Test for Nicotine Dependence as well as measures of other substance use. Three hundred and fifty-one patients (36%) reported current tobacco use, with 227 (65% of all users) reporting moderate to severe nicotine dependence. Current tobacco use as well as nicotine dependence were associated with male gender, a diagnosis of bipolar disorder, and risk of other substance use problems. The cultural context of these findings, and the implications for tobacco control among psychiatric patients, are discussed. JF - Addictive behaviors AU - Chandra, Prabha S AU - Carey, Michael P AU - Carey, Kate B AU - Jairam, K R AU - Girish, N S AU - Rudresh, H P AD - Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bangalore, India. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1290 EP - 1299 VL - 30 IS - 7 SN - 0306-4603, 0306-4603 KW - Index Medicus KW - India -- epidemiology KW - Bipolar Disorder -- epidemiology KW - Hospitalization KW - Epidemiologic Methods KW - Humans KW - Adult KW - Diagnosis, Dual (Psychiatry) KW - Bipolar Disorder -- psychology KW - Aged KW - Middle Aged KW - Adolescent KW - Male KW - Female KW - Tobacco Use Disorder -- epidemiology KW - Mental Disorders -- epidemiology KW - Tobacco Use Disorder -- psychology KW - Mental Disorders -- psychology KW - Developing Countries KW - Smoking -- epidemiology KW - Tobacco, Smokeless UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68038980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addictive+behaviors&rft.atitle=Prevalence+and+correlates+of+tobacco+use+and+nicotine+dependence+among+psychiatric+patients+in+India.&rft.au=Chandra%2C+Prabha+S%3BCarey%2C+Michael+P%3BCarey%2C+Kate+B%3BJairam%2C+K+R%3BGirish%2C+N+S%3BRudresh%2C+H+P&rft.aulast=Chandra&rft.aufirst=Prabha&rft.date=2005-08-01&rft.volume=30&rft.issue=7&rft.spage=1290&rft.isbn=&rft.btitle=&rft.title=Addictive+behaviors&rft.issn=03064603&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-22 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: JAMA. 2000 Nov 22-29;284(20):2606-10 [11086367] Lancet. 1997 May 24;349(9064):1498-504 [9167458] Br J Psychiatry. 2001 Jul;179:35-8 [11435266] Compr Psychiatry. 2001 Sep-Oct;42(5):393-402 [11559866] Br J Psychiatry. 2001 Nov;179:432-7 [11689401] Pharmacol Biochem Behav. 2001 Dec;70(4):561-70 [11796154] Addict Biol. 2002 Jan;7(1):77-83 [11900626] Addict Biol. 2002 Jan;7(1):103-10 [11900629] Schizophr Res. 2002 Jul 1;56(1-2):67-74 [12084421] Psychol Addict Behav. 2003 Dec;17(4):259-65 [14640821] BMJ. 2004 Apr 3;328(7443):801-6 [15070637] Addict Behav. 1982;7(4):363-71 [7183189] JAMA. 1990 Sep 26;264(12):1546-9 [2395194] Br J Addict. 1991 Sep;86(9):1119-27 [1932883] Addiction. 1993 Jun;88(6):791-804 [8329970] Behav Genet. 1995 Mar;25(2):95-101 [7733862] BMJ. 1996 Jun 22;312(7046):1576-9 [8664667] Tob Control. 2001 Jun;10(2):181-3 [11387541] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Antiandrogen, vaccine and combination therapy in patients with nonmetastatic hormone refractory prostate cancer. AN - 68021245; 16006888 AB - There is no current standard treatment for patients with prostate cancer who have received hormonal therapy but have an increasing prostate specific antigen (PSA) without radiographic evidence of metastasis. This trial was designed to analyze toxicity, immunogenicity and time to treatment failure using vaccine, antiandrogen therapy or their sequential use. A total of 42 patients were randomized to receive vaccine vs antiandrogen therapy with nilutamide. The vaccine consisted of recombinant vaccinia viruses containing the PSA and B7.1 costimulatory genes as prime vaccinations, and avipox-PSA as boosters. After 6 months patients with an increasing PSA and no metastasis may receive a combination of both treatments. Three patients on nilutamide were removed from study secondary to grade 3 toxicities but no grade 3 toxicities were attributed to vaccine. In the vaccine arm median time to treatment failure was 9.9 months with 13 of 21 decreases in PSA velocity vs 7.6 months with 16 of 21 decreases in PSA velocity in the nilutamide arm (p =0.28). Of the patients in the nilutamide arm 8 had vaccine added at the time of PSA progression. Median time to treatment failure with combined therapy was 5.2 months, with a median duration from study entry of 15.9 months. Of the patients in the vaccine arm 12 had nilutamide added at the time of PSA progression. Median time to treatment failure with combined therapy was 13.9 months and a median of 25.9 months from initiation of therapy. Further studies are merited to investigate the role of combining vaccine with antiandrogen therapy or vaccine followed by vaccine plus antiandrogen therapy in this patient population. JF - The Journal of urology AU - Arlen, Philip M AU - Gulley, James L AU - Todd, Nushin AU - Lieberman, Ronald AU - Steinberg, Seth M AU - Morin, Steve AU - Bastian, Anne AU - Marte, Jennifer AU - Tsang, Kwong-Yok AU - Beetham, Patricia AU - Grosenbach, Douglas W AU - Schlom, Jeffrey AU - Dahut, William AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 539 EP - 546 VL - 174 IS - 2 SN - 0022-5347, 0022-5347 KW - Androgen Antagonists KW - 0 KW - Cancer Vaccines KW - Imidazolidines KW - nilutamide KW - 51G6I8B902 KW - Prostate-Specific Antigen KW - EC 3.4.21.77 KW - Abridged Index Medicus KW - Index Medicus KW - Aged, 80 and over KW - Humans KW - Prostate-Specific Antigen -- blood KW - Disease Progression KW - Aged KW - Middle Aged KW - Male KW - Prostatic Neoplasms -- mortality KW - Cancer Vaccines -- chemistry KW - Androgen Antagonists -- therapeutic use KW - Cancer Vaccines -- therapeutic use KW - Imidazolidines -- therapeutic use KW - Prostatic Neoplasms -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68021245?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+urology&rft.atitle=Antiandrogen%2C+vaccine+and+combination+therapy+in+patients+with+nonmetastatic+hormone+refractory+prostate+cancer.&rft.au=Arlen%2C+Philip+M%3BGulley%2C+James+L%3BTodd%2C+Nushin%3BLieberman%2C+Ronald%3BSteinberg%2C+Seth+M%3BMorin%2C+Steve%3BBastian%2C+Anne%3BMarte%2C+Jennifer%3BTsang%2C+Kwong-Yok%3BBeetham%2C+Patricia%3BGrosenbach%2C+Douglas+W%3BSchlom%2C+Jeffrey%3BDahut%2C+William&rft.aulast=Arlen&rft.aufirst=Philip&rft.date=2005-08-01&rft.volume=174&rft.issue=2&rft.spage=539&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+urology&rft.issn=00225347&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-16 N1 - Date created - 2005-07-11 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: J Urol. 2005 Aug;174(2):415-6 [16006855] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Handling of dioxin measurement data in the presence of non-detectable values: overview of available methods and their application in the Seveso chloracne study. AN - 67992349; 15992596 AB - Exposure measurements of concentrations that are non-detectable or near the detection limit (DL) are common in environmental research. Proper statistical treatment of non-detects is critical to avoid bias and unnecessary loss of information. In the present work, we present an overview of possible statistical strategies for handling non-detectable values, including deletion, simple substitution, distributional methods, and distribution-based imputation. Simple substitution methods (e.g., substituting 0, DL/2, DL/ radical2, or DL for the non-detects) are the most commonly applied, even though the EPA Guidance for Data Quality Assessment discouraged their use when the percentage of non-detects is >15%. Distribution-based multiple imputation methods, also known as robust or "fill-in" procedures, may produce dependable results even when 50-70% of the observations are non-detects and can be performed using commonly available statistical software. Any statistical analysis can be conducted on the imputed datasets. Results properly reflect the presence of non-detectable values and produce valid statistical inference. We describe the use of distribution-based multiple imputation in a recent investigation conducted on subjects from the Seveso population exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in which 55.6% of plasma TCDD measurements were non-detects. We suggest that distribution-based multiple imputation be the preferred method to analyze environmental data when substantial proportions of observations are non-detects. JF - Chemosphere AU - Baccarelli, Andrea AU - Pfeiffer, Ruth AU - Consonni, Dario AU - Pesatori, Angela C AU - Bonzini, Matteo AU - Patterson, Donald G AU - Bertazzi, Pier Alberto AU - Landi, Maria Teresa AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, 6120 Executive Boulevard, Bethesda, MD 20852, USA. andrea.baccarelli@unimi.it Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 898 EP - 906 VL - 60 IS - 7 SN - 0045-6535, 0045-6535 KW - Polychlorinated Dibenzodioxins KW - 0 KW - Index Medicus KW - Chemistry Techniques, Analytical -- methods KW - Humans KW - Linear Models KW - Data Interpretation, Statistical KW - Acne Vulgaris -- chemically induced KW - Italy KW - Polychlorinated Dibenzodioxins -- blood KW - Environmental Monitoring -- statistics & numerical data UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67992349?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemosphere&rft.atitle=Handling+of+dioxin+measurement+data+in+the+presence+of+non-detectable+values%3A+overview+of+available+methods+and+their+application+in+the+Seveso+chloracne+study.&rft.au=Baccarelli%2C+Andrea%3BPfeiffer%2C+Ruth%3BConsonni%2C+Dario%3BPesatori%2C+Angela+C%3BBonzini%2C+Matteo%3BPatterson%2C+Donald+G%3BBertazzi%2C+Pier+Alberto%3BLandi%2C+Maria+Teresa&rft.aulast=Baccarelli&rft.aufirst=Andrea&rft.date=2005-08-01&rft.volume=60&rft.issue=7&rft.spage=898&rft.isbn=&rft.btitle=&rft.title=Chemosphere&rft.issn=00456535&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Alarmins: chemotactic activators of immune responses. AN - 67976882; 15955682 AB - The recruitment and activation of antigen-presenting cells are critical early steps in mounting an immune response. Many microbial components and endogenous mediators participate in this process. Recent studies have identified a group of structurally diverse multifunctional host proteins that are rapidly released following pathogen challenge and/or cell death and, most importantly, are able to both recruit and activate antigen-presenting cells. These potent immunostimulants, including defensins, cathelicidin, eosinophil-derived neurotoxin, and high-mobility group box protein 1, serve as early warning signals to activate innate and adaptive immune systems. We propose to highlight these proteins' unique activities by grouping them under the novel term 'alarmins', in recognition of their role in mobilizing the immune system. JF - Current opinion in immunology AU - Oppenheim, Joost J AU - Yang, De AD - Laboratory of Molecular Immunoregulation, Center for Cancer Research, Scientific Application and International Cooperation, Inc. (SAIC)-Frederick, National Cancer Institute at Frederick, Frederick, MD 21702, USA. Oppenhei@ncifcrf.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 359 EP - 365 VL - 17 IS - 4 SN - 0952-7915, 0952-7915 KW - Anti-Bacterial Agents KW - 0 KW - Antimicrobial Cationic Peptides KW - Index Medicus KW - Animals KW - Humans KW - Antigen-Presenting Cells -- immunology KW - Infection -- microbiology KW - Anti-Bacterial Agents -- metabolism KW - Anti-Bacterial Agents -- immunology KW - Infection -- immunology KW - Antimicrobial Cationic Peptides -- immunology KW - Antimicrobial Cationic Peptides -- secretion KW - Antimicrobial Cationic Peptides -- metabolism KW - Chemotaxis -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67976882?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+opinion+in+immunology&rft.atitle=Alarmins%3A+chemotactic+activators+of+immune+responses.&rft.au=Oppenheim%2C+Joost+J%3BYang%2C+De&rft.aulast=Oppenheim&rft.aufirst=Joost&rft.date=2005-08-01&rft.volume=17&rft.issue=4&rft.spage=359&rft.isbn=&rft.btitle=&rft.title=Current+opinion+in+immunology&rft.issn=09527915&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-06-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cell death in HIV dementia. AN - 67971980; 15761472 AB - Many patients infected with human immunodeficiency virus type-1 (HIV-1) suffer cognitive impairment ranging from mild to severe (HIV dementia), which may result from neuronal death in the basal ganglia, cerebral cortex and hippocampus. HIV-1 does not kill neurons by infecting them. Instead, viral proteins released from infected glial cells, macrophages and/or stem cells may directly kill neurons or may increase their vulnerability to other cell death stimuli. By binding to and/or indirectly activating cell surface receptors such as CXCR4 and the N-methyl-D-aspartate receptor, the HIV-1 proteins gp120 and Tat may trigger neuronal apoptosis and excitotoxicity as a result of oxidative stress, perturbed cellular calcium homeostasis and mitochondrial alterations. Membrane lipid metabolism and inflammation may also play important roles in determining whether neurons live or die in HIV-1-infected patients. Drugs and diets that target oxidative stress, excitotoxicity, inflammation and lipid metabolism are in development for the treatment of HIV-1 patients. JF - Cell death and differentiation AU - Mattson, M P AU - Haughey, N J AU - Nath, A AD - Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. mattsonm@grc.nia.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 893 EP - 904 VL - 12 Suppl 1 SN - 1350-9047, 1350-9047 KW - Anti-Retroviral Agents KW - 0 KW - Gene Products, tat KW - HIV Envelope Protein gp120 KW - Inflammation Mediators KW - Receptors, Glutamate KW - Viral Proteins KW - tat Gene Products, Human Immunodeficiency Virus KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Virus Replication KW - Animals KW - Neurons -- metabolism KW - Apoptosis KW - Neurons -- drug effects KW - Humans KW - Viral Proteins -- pharmacology KW - Receptors, Glutamate -- metabolism KW - Neuroglia -- drug effects KW - Viral Proteins -- toxicity KW - Gene Products, tat -- pharmacology KW - Anti-Retroviral Agents -- therapeutic use KW - Neurons -- pathology KW - Inflammation Mediators -- metabolism KW - Calcium -- metabolism KW - HIV Envelope Protein gp120 -- pharmacology KW - Neuroglia -- metabolism KW - Oxidative Stress KW - Cell Death KW - Lipid Metabolism KW - HIV-1 -- genetics KW - AIDS Dementia Complex -- genetics KW - AIDS Dementia Complex -- drug therapy KW - AIDS Dementia Complex -- metabolism KW - HIV-1 -- pathogenicity KW - Brain -- pathology KW - Brain -- virology KW - Brain -- metabolism KW - HIV-1 -- physiology KW - AIDS Dementia Complex -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67971980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+death+and+differentiation&rft.atitle=Cell+death+in+HIV+dementia.&rft.au=Mattson%2C+M+P%3BHaughey%2C+N+J%3BNath%2C+A&rft.aulast=Mattson&rft.aufirst=M&rft.date=2005-08-01&rft.volume=12+Suppl+1&rft.issue=&rft.spage=893&rft.isbn=&rft.btitle=&rft.title=Cell+death+and+differentiation&rft.issn=13509047&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-23 N1 - Date created - 2005-06-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serum Erythropoietin and Aging: A Longitudinal Analysis AN - 57084113; 200600242 AB - OBJECTIVES: To determine the changes in serum erythropoietin with age in patients with and without anemia and to assess the importance of certain comorbidities on changes in erythropoietin level and the development of anemia. DESIGN: Clinical history, hematological parameters, and serum erythropoietin levels were examined at 1- to 2-year intervals for 8 to 30 years. SETTING: Baltimore Longitudinal Study on Aging (BLSA), National Institute on Aging. PARTICIPANTS: One hundred forty-three BLSA participants. MEASUREMENTS: Complete blood count and serum chemistries were performed at the time of each visit, and archived serum samples were used for erythropoietin level. RESULTS: Although all subjects were healthy and without anemia at the time of initial evaluation, some developed chronic illness - most notably hypertension and diabetes mellitus. Erythropoietin levels rose significantly for the group as a whole, and the slope of the rise was found to be greater for those who did not have associated diabetes mellitus or hypertension. During the subsequent years, subjects who developed anemia but did not have hypertension or diabetes mellitus had the greatest slope in erythropoietin rise over time, whereas those with hypertension or diabetes mellitus and anemia had the lowest erythropoietin slope. CONCLUSION: The increase in serum erythropoietin with aging may be compensation for subclinical blood loss, increased red blood cell turnover, or increased erythropoietin resistance of red cell precursors. It is suspected that, with very advanced age, or in those with compromised renal function (e.g., diabetes mellitus or hypertension), the compensatory mechanism becomes inadequate and anemia results. Illustrations, Tables, References. Adapted from the source document. JF - Journal of the American Geriatrics Society AU - Ershler, William B AU - Sheng, Shan AU - McKelvey, Julie AU - Artz, Andrew S AU - Denduluri, Neelima AU - Tecson, Josephine AU - Taub, Dennis D AU - Brant, Larry J AU - Ferrucci, Luigi AU - Longo, Dan L AD - Instit Advanced Studies Aging, Washington, DC ershlerwi@grc.nia.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1360 EP - 1365 PB - JAGSAF VL - 53 IS - 8 SN - 0002-8614, 0002-8614 KW - erythropoietin KW - anemia KW - BLSA KW - aging KW - Longitudinal studies KW - Ageing KW - Anaemia KW - Hormones KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57084113?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Geriatrics+Society&rft.atitle=Serum+Erythropoietin+and+Aging%3A+A+Longitudinal+Analysis&rft.au=Ershler%2C+William+B%3BSheng%2C+Shan%3BMcKelvey%2C+Julie%3BArtz%2C+Andrew+S%3BDenduluri%2C+Neelima%3BTecson%2C+Josephine%3BTaub%2C+Dennis+D%3BBrant%2C+Larry+J%3BFerrucci%2C+Luigi%3BLongo%2C+Dan+L&rft.aulast=Ershler&rft.aufirst=William&rft.date=2005-08-01&rft.volume=53&rft.issue=8&rft.spage=1360&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Geriatrics+Society&rft.issn=00028614&rft_id=info:doi/10.1111%2Fj.1532-5415.2005.53416.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JAGSAF N1 - SubjectsTermNotLitGenreText - Anaemia; Ageing; Longitudinal studies; Hormones DO - http://dx.doi.org/10.1111/j.1532-5415.2005.53416.x ER - TY - JOUR T1 - Choosing healthplans all together: a deliberative exercise for allocating limited health care resources AN - 37744722; 3275667 AB - CHAT (Choosing Healthplans All Together) is an exercise in participatory decision making designed to engage the public in health care priority setting. Participants work individually and then in groups to distribute a limited number of pegs on a board as they select from a wide range of insurance options. Randomly distributed health events illustrate the consequences of insurance choices. In 1999-2000, the authors conducted fifty sessions of CHAT involving 592 residents of North Carolina. The exercise was rated highly regarding ease of use, informativeness, and enjoyment. Participants found the information believable and complete, thought the group decision-making process was fair, and were willing to abide by group decisions. CHAT holds promise as a tool to foster group deliberation, generate collective choices, and incorporate the preferences and values of consumers into allocation decisions. It can serve to inform and stimulate public dialogue about limited health care resources. Reprinted by permission of Duke University Press JF - Journal of health politics, policy and law AU - Goold, Susan Dorr AU - Biddle, Andrea K AU - Klipp, Glenn AU - Hall, Charles N AU - Danis, Marion AD - University of Michigan ; University of North Carolina ; National Institutes of Health Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 563 EP - 602 VL - 30 IS - 4 SN - 0361-6878, 0361-6878 KW - Political Science KW - U.S.A. KW - Decision making KW - Health care KW - Social participation KW - North Carolina KW - Health insurance KW - Health policy KW - Law KW - Dialogue UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37744722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+health+politics%2C+policy+and+law&rft.atitle=Choosing+healthplans+all+together%3A+a+deliberative+exercise+for+allocating+limited+health+care+resources&rft.au=Goold%2C+Susan+Dorr%3BBiddle%2C+Andrea+K%3BKlipp%2C+Glenn%3BHall%2C+Charles+N%3BDanis%2C+Marion&rft.aulast=Goold&rft.aufirst=Susan&rft.date=2005-08-01&rft.volume=30&rft.issue=4&rft.spage=563&rft.isbn=&rft.btitle=&rft.title=Journal+of+health+politics%2C+policy+and+law&rft.issn=03616878&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 5775 13521; 3322 6071 1542 11325; 11880 11878 9003; 5784 6592 4957 11923 11949 13521; 3532 2572; 7253; 5788 11888 10472; 294 433 293 14 ER - TY - JOUR T1 - Neuroeconomics AN - 37727609; 3263818 JF - Games and economic behavior AU - Glimcher, Paul W AU - Dorris, Michael C AU - Bayer, Hannah M AU - Rustichini, Aldo AU - Dickhaut, John AU - Ghirardato, Paolo AU - Smith, Kip AU - Pardo, Josè V AU - Conover, Kent L AU - Shizgal, Peter AU - Knutson, Brian AU - Peterson, Richard AU - Bechara, Antoine AU - Damasio, Antonio R AU - Houser, Daniel AU - Keane, Michael AU - McCabe, Kevin AU - Smith, Vernon AU - Leland, Jonathan W AU - Grafman, Jordan AU - Gallistel, C R AU - Bhatt, Meghana AU - Camerer, Colin F AU - Benhabib, Jess AU - Bisin, Alberto AD - New York University ; University of Minnesota ; Università di Torino ; Concordia University ; Stanford University ; University of Iowa ; George Mason University ; Yale University ; National Science Foundation ; National Institute of Neurological Disorders and Stroke ; Rutgers University ; California Institute of Technology Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 201 EP - 493 VL - 52 IS - 2 SN - 0899-8256, 0899-8256 KW - Economics KW - U.S.A. KW - Empirical tests KW - Emotions KW - Institutionalism KW - Model testing KW - Savings KW - Game theory KW - Attitude formation KW - Algorithms KW - Laboratory KW - Review articles KW - Economic psychology KW - Auctions KW - Microeconomics KW - Human behaviour KW - Economic theory KW - Experimental methods KW - Utility functions KW - Brain KW - Neuroscience KW - Vermont KW - Decision making KW - Neuropsychology KW - Economic behaviour KW - Consumption KW - Lotteries KW - Economic analysis KW - Labour supply KW - Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37727609?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+Developmental+Science&rft.atitle=Parenting%2C+culture%2C+and+context%3A+Reflections+on+excavating+culture&rft.au=Cauce%2C+Ana+Mari&rft.aulast=Cauce&rft.aufirst=Ana&rft.date=2008-10-01&rft.volume=12&rft.issue=4&rft.spage=227&rft.isbn=&rft.btitle=&rft.title=Applied+Developmental+Science&rft.issn=10888691&rft_id=info:doi/10.1080%2F10888690802388177 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - SuppNotes - Collection of 11 articles N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 5403 8010 4025; 8010 4025; 8637; 8636; 4019; 3883 971; 6071 1542 11325; 3322 6071 1542 11325; 1615 8573 11325; 1750 1678; 7548 5401 7336 3198; 4202; 3889 6071 1542 11325; 7180 12401; 13219 13221; 4196; 3985 10404; 7127 13673 4214; 1385; 6588; 10999; 918 7824; 8160 8163; 1373 1378 10404; 4631; 2805 3872 554 971; 11280 8235; 447 433 293 14 ER - TY - JOUR T1 - Experimental tests of the somatic marker hypothesis AN - 37724906; 3263368 JF - Games and economic behavior AU - Leland, Jonathan W AU - Grafman, Jordan AD - National Science Foundation ; National Institute of Neurological Disorders and Stroke Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 386 EP - 409 VL - 52 IS - 2 SN - 0899-8256, 0899-8256 KW - Economics KW - Experiments KW - Decision making KW - Neuropsychology KW - Economic theory KW - Model testing KW - Brain KW - Microeconomics KW - Neuroscience KW - Human behaviour UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37724906?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Games+and+economic+behavior&rft.atitle=Experimental+tests+of+the+somatic+marker+hypothesis&rft.au=Leland%2C+Jonathan+W%3BGrafman%2C+Jordan&rft.aulast=Leland&rft.aufirst=Jonathan&rft.date=2005-08-01&rft.volume=52&rft.issue=2&rft.spage=386&rft.isbn=&rft.btitle=&rft.title=Games+and+economic+behavior&rft.issn=08998256&rft_id=info:doi/10.1016%2Fj.geb.2004.09.001 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 8010 4025; 4019; 6071 1542 11325; 8636; 8637; 4636 6845 6564 12622; 1750 1678; 3322 6071 1542 11325; 8160 8163 DO - http://dx.doi.org/10.1016/j.geb.2004.09.001 ER - TY - JOUR T1 - Phase II study of CCI-779 in patients with recurrent glioblastoma multiforme AN - 216498628; 16012795 AB - Purpose: Loss of PTEN, which is common in glioblastoma multiforme (GBM), results in activation of the mammalian target of rapapmycin (mTOR), thereby increasing mRNA translation of a number of key proteins required for cell-cycle progression. CCI-779 is an inhibitor of mTOR. The primary objectives of this study were to determine the efficacy of CCI-779 in patients with recurrent GBM and to further assess the toxicity of the drug. Experimental Design: CCI-779 was administered weekly at a dose of 250 mg intravenously for patients on enzyme-inducing anti-epileptic drugs (EIAEDs). Patients not on EIAEDs were initially treated at 250 mg; however, the dose was reduced to 170 mg because of intolerable side effects. Treatment was continued until unacceptable toxicity, tumor progression, or patient withdrawal. The primary endpoint was 6-month progression-free survival. Results: Forty-three patients were enrolled; 29 were not on EIAEDs. The expected toxicity profile of increased lipids, lymphopenia, and stomatitis was seen. There were no grade IV hematological toxicities and no toxic deaths. One patient was progression free at 6 months. Of the patients assessable for response, there were 2 partial responses and 20 with stabilization of disease. The median time to progression was 9 weeks. Conclusions: CCI-779 was well tolerated at this dose schedule; however, there was no evidence of efficacy in patients with recurrent GBM. Despite initial disease stabilization in approximately 50% of patients, the durability of response was short. Because of the low toxicity profile, CCI-779 may merit exploration in combination with other modalities. JF - Investigational New Drugs AU - Chang, Susan M AU - Wen, Patrick AU - Cloughesy, Timothy AU - Greenberg, Harry AU - Schiff, David AU - Conrad, Charles AU - Fink, Karen AU - Robins, H Ian AU - De Angelis, Lisa AU - Raizer, Jeffrey AU - Hess, Kenneth AU - Aldape, Ken AU - Lamborn, Kathleen R AU - Kuhn, John AU - Dancey, Janet AU - Prados, Michael D Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 357 EP - 61 CY - New York PB - Springer Science & Business Media VL - 23 IS - 4 SN - 01676997 KW - Pharmacy And Pharmacology KW - Anticonvulsants KW - Antineoplastic Agents KW - temsirolimus KW - Sirolimus KW - Disease-Free Survival KW - Drug Interactions KW - Lymphopenia -- chemically induced KW - Infusions, Intravenous KW - Humans KW - Anemia -- chemically induced KW - Aged KW - Anticonvulsants -- administration & dosage KW - Sirolimus -- therapeutic use KW - Anticonvulsants -- therapeutic use KW - Antineoplastic Agents -- adverse effects KW - Sirolimus -- adverse effects KW - Adult KW - Middle Aged KW - Hypercholesterolemia -- chemically induced KW - Male KW - Female KW - Brain Neoplasms -- drug therapy KW - Neoplasm Recurrence, Local -- drug therapy KW - Glioblastoma -- drug therapy KW - Antineoplastic Agents -- therapeutic use KW - Sirolimus -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/216498628?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aabiglobal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Investigational+New+Drugs&rft.atitle=Phase+II+study+of+CCI-779+in+patients+with+recurrent+glioblastoma+multiforme&rft.au=Chang%2C+Susan+M%3BWen%2C+Patrick%3BCloughesy%2C+Timothy%3BGreenberg%2C+Harry%3BSchiff%2C+David%3BConrad%2C+Charles%3BFink%2C+Karen%3BRobins%2C+H+Ian%3BDe+Angelis%2C+Lisa%3BRaizer%2C+Jeffrey%3BHess%2C+Kenneth%3BAldape%2C+Ken%3BLamborn%2C+Kathleen+R%3BKuhn%2C+John%3BDancey%2C+Janet%3BPrados%2C+Michael+D&rft.aulast=Chang&rft.aufirst=Susan&rft.date=2005-08-01&rft.volume=23&rft.issue=4&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Investigational+New+Drugs&rft.issn=01676997&rft_id=info:doi/10.1007%2Fs10637-005-1444-0 LA - English DB - ProQuest Central N1 - Copyright - Springer Science + Business Media, Inc. 2005 N1 - Last updated - 2014-04-30 N1 - CODEN - INNDDK DO - http://dx.doi.org/10.1007/s10637-005-1444-0 ER - TY - JOUR T1 - CpG Immunomer DNA Enhances Antisense Protein Kinase A RI alpha Inhibition of Multidrug-Resistant Colon Carcinoma Growth in Nude Mice: Molecular Basis for Combinatorial Therapy AN - 21141712; 6522362 AB - PURPOSE: CpG DNAs induce cytokines, activate natural killer cells, and elicit vigorous T-cell response leading to antitumor effects. Antisense oligodeoxynucleotides targeted against the RI alpha subunit of protein kinase A (antisense PKA RI alpha ) induce growth arrest, apoptosis, and differentiation in a variety of cancer cell lines in vitro and in vivo. This study investigated the use of a combinatorial therapy consisting of the RNA-DNA second-generation antisense PKA RI alpha and the CpG immunomer (CpG DNA linked through 3'-3' linkage containing two accessible 5' ends). Experimental Design: HCT-15 multidrug-resistant colon carcinoma growth in nude mice was used as an experimental model. The inhibitory effect on tumor growth and apoptotic activity of antisense RI alpha and CpG immunomer, singly and in combination, were measured by tumor growth, levels of RI alpha subunit, and antiapoptotic and proapoptotic proteins. Effect on host-immune system was measured by mouse spleen size, interleukin-6 (IL-6) levels in mouse blood, and nuclear factor- Kappa B (NF- Kappa B) transcription activity in mouse spleen cells. RESULTS: In combination, CpG immunomer and antisense PKA RI alpha induced additive/supra-additive effect on the inhibition of tumor growth. Antisense RI alpha but not CpG immunomer increased Bax and Bak proapoptotic protein levels and decreased Bcl-2 and RI alpha protein levels in tumor cells. CpG immunomer but not antisense RI alpha induced an enlargement of mouse spleen, increased IL-6 levels in mouse blood, and increased NF- Kappa B transcription activity in mouse spleen cells. CONCLUSIONS: These results show that type I PKA down-regulation and induction of apoptosis in tumor cells by antisense PKA RI alpha , and host-immune stimulation by CpG immunomer are responsible at the molecular level for the supra-additive effects of tumor growth inhibition. Thus, antisense PKA RI alpha and CpG immunomer in combination work cooperatively and as tumor-targeted therapeutics to treat human cancer. JF - Clinical Cancer Research AU - Nesterova, Maria V AU - Johnson, Natalie R AU - Stewart, Trina AU - Abrams, Scott AU - Cho-Chung, Yoon S AD - Authors' Affiliations: Basic Research Laboratory, Cellular Biochemistry Section and Laboratory of Tumor, Immunology and Biology, National Cancer Institute, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5950 EP - 5955 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 16 SN - 1078-0432, 1078-0432 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Interleukin 6 KW - Protein kinase A KW - Apoptosis KW - Drug resistance KW - Animal models KW - Tumor cells KW - NF- Kappa B protein KW - Antisense oligonucleotides KW - Differentiation KW - Tumor cell lines KW - Colon KW - Lymphocytes T KW - Cytokines KW - Bcl-2 protein KW - Antisense RNA KW - Natural killer cells KW - Antisense DNA KW - Spleen KW - Transcription KW - CpG islands KW - Tumors KW - Blood KW - Bax protein KW - BAK protein KW - Antitumor activity KW - N 14830:RNA KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21141712?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=CpG+Immunomer+DNA+Enhances+Antisense+Protein+Kinase+A+RI+alpha+Inhibition+of+Multidrug-Resistant+Colon+Carcinoma+Growth+in+Nude+Mice%3A+Molecular+Basis+for+Combinatorial+Therapy&rft.au=Nesterova%2C+Maria+V%3BJohnson%2C+Natalie+R%3BStewart%2C+Trina%3BAbrams%2C+Scott%3BCho-Chung%2C+Yoon+S&rft.aulast=Nesterova&rft.aufirst=Maria&rft.date=2005-08-01&rft.volume=11&rft.issue=16&rft.spage=5950&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Interleukin 6; Protein kinase A; Apoptosis; Drug resistance; Animal models; Tumor cells; NF- Kappa B protein; Differentiation; Antisense oligonucleotides; Tumor cell lines; Colon; Lymphocytes T; Cytokines; Bcl-2 protein; Antisense RNA; Natural killer cells; Transcription; Spleen; Antisense DNA; Tumors; CpG islands; Blood; Bax protein; BAK protein; Antitumor activity ER - TY - JOUR T1 - The Youden Index and the Optimal Cut-Point Corrected for Measurement Error AN - 21122445; 11157971 AB - Random measurement error can attenuate a biomarker's ability to discriminate between diseased and non-diseased populations. A global measure of biomarker effectiveness is the Youden index, the maximum difference between sensitivity, the probability of correctly classifying diseased individuals, and 1-specificity, the probability of incorrectly classifying health individuals. We present an approach for estimating the Youden index and associated optimal cut-point for a normally distributed biomarker that corrects for normally distributed random measurement error. We also provide confidence intervals for these corrected estimates using the delta method and coverage probability through simulation over a variety of situations. Applying these techniques to the biomarker thiobarbituric acid reaction substance (TBARS), a measure of sub-products of lipid peroxidation that has been proposed as a discriminating measurement for cardiovascular disease, yields a 50% increase in diagnostic effectiveness at the optimal cut-point. This result may lead to biomarkers that were once naively considered ineffective becoming useful diagnostic devices. JF - Biometrical Journal AU - Perkins, Neil J AU - Schisterman, Enrique F AD - Division of Epidemiology, Statistics and Prevention Research, National Institute of Child Health and Human Development (NICHD), National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA, schistee@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 428 EP - 441 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 47 IS - 4 SN - 0323-3847, 0323-3847 KW - Biotechnology and Bioengineering Abstracts KW - thiobarbituric acid KW - Statistics KW - Cardiovascular diseases KW - Biometrics KW - biomarkers KW - Lipid peroxidation KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21122445?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biometrical+Journal&rft.atitle=The+Youden+Index+and+the+Optimal+Cut-Point+Corrected+for+Measurement+Error&rft.au=Perkins%2C+Neil+J%3BSchisterman%2C+Enrique+F&rft.aulast=Perkins&rft.aufirst=Neil&rft.date=2005-08-01&rft.volume=47&rft.issue=4&rft.spage=428&rft.isbn=&rft.btitle=&rft.title=Biometrical+Journal&rft.issn=03233847&rft_id=info:doi/10.1002%2Fbimj.200410133 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - biomarkers; Biometrics; thiobarbituric acid; Statistics; Cardiovascular diseases; Lipid peroxidation DO - http://dx.doi.org/10.1002/bimj.200410133 ER - TY - JOUR T1 - Plasmodium falciparum: Characterization of a late asexual stage Golgi protein containing both ankyrin and DHHC domains AN - 20941878; 8250964 AB - Proteins containing the DHHC motif have been shown to function as palmitoyl transferases. The palmitoylation of proteins has been shown to play an important role in the trafficking of proteins to the proper subcellular location. Herein, we describe a protein containing both ankyrin domains and a DHHC domain that is present in the Golgi of late schizonts of P. falciparum. The timing of expression as well as the location of this protein suggests that it may play an important role in the sorting of proteins to the apical organelles during the development of the asexual stage of the parasite. JF - Experimental Parasitology AU - Seydel, K B AU - Gaur, D AU - Aravind, L AU - Subramanian, G AU - Miller, L H AD - National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA, lmiller@niaid.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 389 EP - 393 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl] VL - 110 IS - 4 SN - 0014-4894, 0014-4894 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources KW - Protein transport KW - Golgi apparatus KW - Parasites KW - Transferases KW - Schizonts KW - Developmental stages KW - Plasmodium falciparum KW - Endoparasites KW - palmitoylation KW - Ankyrin KW - Proteins KW - Organelles KW - Q1 08484:Species interactions: parasites and diseases KW - Q5 08501:General KW - K 03320:Cell Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20941878?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+Parasitology&rft.atitle=Plasmodium+falciparum%3A+Characterization+of+a+late+asexual+stage+Golgi+protein+containing+both+ankyrin+and+DHHC+domains&rft.au=Seydel%2C+K+B%3BGaur%2C+D%3BAravind%2C+L%3BSubramanian%2C+G%3BMiller%2C+L+H&rft.aulast=Seydel&rft.aufirst=K&rft.date=2005-08-01&rft.volume=&rft.issue=&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Boston+Globe+%28pre-1997+Fulltext%29&rft.issn=07431791&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Parasites; Transferases; Proteins; Developmental stages; Endoparasites; Golgi apparatus; Protein transport; palmitoylation; Ankyrin; Schizonts; Organelles; Plasmodium falciparum DO - http://dx.doi.org/10.1016/j.exppara.2005.03.030 ER - TY - JOUR T1 - Role of withdrawal in reinstatement of morphine-conditioned place preference AN - 20887827; 6886198 AB - Rationale: Relapse is a major characteristic of drug addiction and the primary problem in treating drug abuse. Based on the negative reinforcement view of addiction, in which the motivation to take drugs is thought to result from the desire to avoid the aversive effect of drug withdrawal, it has been theorized that withdrawal symptoms play a major role in the maintenance of and relapse to drug taking. However, the role of withdrawal in relapse has not yet been systemically investigated in the reinstatement model. Objectives: Using a conditioned place preference (CPP) paradigm, we examined the role of different morphine withdrawal states (spontaneous withdrawal, naloxone-precipitated withdrawal, and conditioned withdrawal) in relapse to drug seeking. Methods: Rats alternately received morphine (10 mg/kg, s.c.) and saline for 8 days to acquire the CPP. The morphine CPP disappeared after a 2-week extinction phase of saline-paired training. Rats were then chronically administered morphine to induce physical dependence. The different withdrawal states were induced and their roles in the reinstatement of extinguished CPP were assessed. During conditioned withdrawal, trunk blood samples were taken and the corticosterone level was measured by radioimmunoassay. To examine the role of corticotropin-releasing factor (CRF) receptor antagonist on conditioned-withdrawal-induced reinstatement of CPP, different doses of alpha -helical CRF (0.1 and 1 mu g, i.c.v.) were administered 30 min prior to the CPP testing. Results: The results show that morphine spontaneous withdrawal and naloxone-precipitated morphine withdrawal were ineffective in reinstating morphine CPP. However, the withdrawal cues significantly elicited the reinstatement of CPP and increased corticosterone level. Moreover, pretreatment with the CRF receptor antagonist alpha -helical CRF (1 mu g, i.c.v.) significantly attenuated the effects of withdrawal cues on reinstatement of CPP and corticosterone levels. Conclusion: These findings demonstrate that the cues associated with previous drug withdrawal play a major role in drug relapse and that activation of the CRF receptor is involved in conditioned-withdrawal-induced reinstatement. The present study suggests that CRF receptor antagonists might be of value in the treatment and prevention of relapse to drug seeking after long-term abstinence. JF - Psychopharmacology AU - Lu, Lin AU - Chen, Hai AU - Su, Wenjuan AU - Ge, Xin AU - Yue, Wen AU - Su, Fen AU - Ma, Lan AD - IRP/NIDA/NIH, 5500 Nathan Shock Drive, Baltimore, MD, 21224, USA, llu@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 90 EP - 100 PB - Springer-Verlag (Berlin), Heidelberger Platz 3 Berlin 14197 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 181 IS - 1 SN - 0033-3158, 0033-3158 KW - Animal Behavior Abstracts; Toxicology Abstracts; CSA Neurosciences Abstracts KW - Place preferences KW - Morphine KW - Extinction KW - Motivation KW - Withdrawal KW - Drug abuse KW - Reinstatement KW - Radioimmunoassay KW - Physical training KW - Corticosterone KW - Reinforcement KW - Drug addiction KW - Drugs KW - Corticotropin-releasing hormone KW - N3 11001:Behavioral and Cognitive Neuroscience KW - Y 25811:General KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20887827?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Role+of+withdrawal+in+reinstatement+of+morphine-conditioned+place+preference&rft.au=Lu%2C+Lin%3BChen%2C+Hai%3BSu%2C+Wenjuan%3BGe%2C+Xin%3BYue%2C+Wen%3BSu%2C+Fen%3BMa%2C+Lan&rft.aulast=Lu&rft.aufirst=Lin&rft.date=2005-08-01&rft.volume=181&rft.issue=1&rft.spage=90&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/10.1007%2Fs00213-005-2207-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Morphine; Place preferences; Motivation; Extinction; Withdrawal; Drug abuse; Radioimmunoassay; Reinstatement; Physical training; Corticosterone; Reinforcement; Drug addiction; Corticotropin-releasing hormone; Drugs DO - http://dx.doi.org/10.1007/s00213-005-2207-5 ER - TY - JOUR T1 - High abundance protein profiling of cystic fibrosis lung epithelial cells AN - 20859190; 6243359 AB - Protein profiles of cultured cystic fibrosis (CF) lung epithelial cells were analyzed by two-dimensional gel electrophoresis and mass spectrometry (MS). The analysis gave rise to a protein map over the pI range of 4-7, and a molecular weight range of ca. 100-10 kDa. The map contains 194 identified proteins, which were detectable by silver stain. All silver stained features were identified by matrix-assisted laser desorption/ionization-time of flight MS of tryptic peptides. Some proteins were found to be represented by multiple features on the 2-D gel. Among the high abundance proteins identified were sets of proteins associated with inflammation, including the classical NF Kappa B, p65 (RelA) and NF Kappa B, p65 (RelB). We suggest that this composite atlas of the high abundance CF lung epithelial proteome will serve as a reference database for future studies of candidate CF drugs, validating different approaches to CFTR gene therapy, and analogous investigations of other types of human lung disorders. JF - Proteomics AU - Pollard, Harvey B AU - Ji, Xiao-duo AU - Jozwik, Catherine AU - Jacobowitz, David M AD - Department of Anatomy, Physiology and Genetics, and Institute for Molecular Medicine, Uniformed Services University School of Medicine, USUHS, Bethesda, MD, USA, dwj@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2210 EP - 2226 PB - Wiley-VCH, Postfach 101161 Weinheim 69451 Germany, [mailto:info@wiley-vch.de], [URL:http://www.wiley-vch.de/publish/en/] VL - 5 IS - 8 SN - 1615-9853, 1615-9853 KW - Biotechnology and Bioengineering Abstracts KW - Cystic fibrosis KW - Epithelial cells KW - Lung KW - Protein KW - Two-dimensional gel electrophoresis KW - RelB protein KW - Cystic fibrosis transmembrane conductance regulator KW - Drug development KW - Stains KW - RelA protein KW - Mass spectroscopy KW - Gel electrophoresis KW - Inflammation KW - Flight KW - Databases KW - Atlases KW - Molecular weight KW - Lasers KW - Tryptic peptides KW - proteomics KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20859190?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proteomics&rft.atitle=High+abundance+protein+profiling+of+cystic+fibrosis+lung+epithelial+cells&rft.au=Pollard%2C+Harvey+B%3BJi%2C+Xiao-duo%3BJozwik%2C+Catherine%3BJacobowitz%2C+David+M&rft.aulast=Pollard&rft.aufirst=Harvey&rft.date=2005-08-01&rft.volume=5&rft.issue=8&rft.spage=2210&rft.isbn=&rft.btitle=&rft.title=Proteomics&rft.issn=16159853&rft_id=info:doi/10.1002%2Fpmic.200401120 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-09-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - RelB protein; Epithelial cells; Cystic fibrosis transmembrane conductance regulator; Drug development; Stains; RelA protein; Gel electrophoresis; Mass spectroscopy; Inflammation; Flight; Databases; Atlases; Lung; Molecular weight; Tryptic peptides; Lasers; proteomics; Cystic fibrosis DO - http://dx.doi.org/10.1002/pmic.200401120 ER - TY - JOUR T1 - Modeling and simulations of the pharmacokinetics of fluorophore conjugated antibodies in tumor vicinity for the optimization of fluorescence-based optical imaging AN - 20490620; 8029912 AB - Background and Objectives One of the methods to detect and localize tumors in tissue is to use fluorophore conjugated specific antibodies as tumor surface markers. The goals of this study are to understand and quantify the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor. This study concludes another stage of the development of a non-invasive fluorescenated antibody-based technique for imaging and localization of tumors in vivo. Study Design/Materials and Methods A mathematical model of the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor was developed based on histological staining experiments. We present the model equations of concentrations of antibodies and free binding sites. We also present a powerful simulation tool that we developed to simulate the imaging process. We analyzed the model and studied the effects of various independent parameters on the imaging result. These parameters included initial volume of markers (injected volume), total number of binding sites, tumor size, binding and dissociation rate constants, and the diffusion coefficient. We present the relations needed between these parameters in order to optimize the imaging results. Results and Conclusions A powerful and accurate tool was developed which may assist in optimizing the imaging system results by setting the injection volume and concentration of fluorophore conjugated antibodies in tissue and approximating the time interval where maximum specific binding occurs and the tumor can be imaged. Lasers Surg. Med. JF - Lasers in Surgery and Medicine AU - Fibich, G AU - Hammer, A AU - Gannot, G AU - Gandjbakhche, A AU - Gannot, I AD - Department of Applied Mathematics, Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel, gannoti@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 155 EP - 160 PB - John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/] VL - 37 IS - 2 SN - 0196-8092, 0196-8092 KW - Biotechnology and Bioengineering Abstracts KW - Antibodies KW - Mathematical models KW - Developmental stages KW - Lasers KW - Tumors KW - fluorophores KW - Diffusion coefficient KW - imaging KW - Pharmacokinetics KW - Surface markers KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20490620?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lasers+in+Surgery+and+Medicine&rft.atitle=Modeling+and+simulations+of+the+pharmacokinetics+of+fluorophore+conjugated+antibodies+in+tumor+vicinity+for+the+optimization+of+fluorescence-based+optical+imaging&rft.au=Fibich%2C+G%3BHammer%2C+A%3BGannot%2C+G%3BGandjbakhche%2C+A%3BGannot%2C+I&rft.aulast=Fibich&rft.aufirst=G&rft.date=2005-08-01&rft.volume=37&rft.issue=2&rft.spage=155&rft.isbn=&rft.btitle=&rft.title=Lasers+in+Surgery+and+Medicine&rft.issn=01968092&rft_id=info:doi/10.1002%2Flsm.20200 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Antibodies; Mathematical models; Developmental stages; Lasers; Diffusion coefficient; fluorophores; Tumors; imaging; Surface markers; Pharmacokinetics DO - http://dx.doi.org/10.1002/lsm.20200 ER - TY - JOUR T1 - DNA Drug Design for Cancer Therapy AN - 20304165; 7653589 AB - DNA (antisense and other oligonucleotides) drug design represents a direct genetic approach for cancer treatment. Such an approach takes advantage of mechanisms that activate genes known to confer a growth advantage to neoplastic cells. The ability to block the expression of these genes allows exploration of normal growth regulation. Progress in DNA drug technology has been rapid, and the traditional antisense inhibition of gene expression is now viewed on a genomic scale. This global view has led to a new vision in antisense technology, the elimination of nonspecific and undesirable side effects, and ultimately the generation of more effective and less toxic nucleic acid medicines. Several antisense oligonucleotides are in clinical trials, are well tolerated, and are potentially active therapeutically. DNA drugs are promising molecular medicines for treating human cancer in the near future. JF - Current Pharmaceutical Design AU - Cho-Chung, Y S AD - Cellular Biochemistry Section, BRL, CCR, National Cancer Institute, Bldg. 10, Rm. 5B05, 9000 Rockville Pike, Bethesda, MD 20892-1750, USA. Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2811 EP - 2823 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 11 IS - 22 SN - 1381-6128, 1381-6128 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - antisense KW - oligonucleotides KW - dna drugs KW - gene expression KW - cancer KW - growth inhibition KW - transcription factor decoy KW - Antisense oligonucleotides KW - nucleic acids KW - Vision KW - Antisense DNA KW - Drug development KW - genomics KW - Clinical trials KW - Cancer KW - Side effects KW - N 14840:Antisense, Nucleotide Analogs KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20304165?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Pharmaceutical+Design&rft.atitle=DNA+Drug+Design+for+Cancer+Therapy&rft.au=Cho-Chung%2C+Y+S&rft.aulast=Cho-Chung&rft.aufirst=Y&rft.date=2005-08-01&rft.volume=11&rft.issue=22&rft.spage=2811&rft.isbn=&rft.btitle=&rft.title=Current+Pharmaceutical+Design&rft.issn=13816128&rft_id=info:doi/10.2174%2F1381612054546770 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Antisense oligonucleotides; nucleic acids; Vision; Antisense DNA; Drug development; genomics; Clinical trials; Side effects; Cancer DO - http://dx.doi.org/10.2174/1381612054546770 ER - TY - JOUR T1 - Beyond Single Pathway Inhibition: MEK Inhibitors as a Platform for the Development of Pharmacological Combinations with Synergistic Anti-Leukemic Effects AN - 20281482; 7653586 AB - The MEK/MAPK signaling module is a key integration point along signal transduction cascades that regulate cell growth, survival, and differentiation, and is aberrantly activated in many human tumors. In tumor cells, constitutive MAPK activation affords increased proliferation and resistance to apoptotic stimuli, including classical cytotoxic drugs. In most instances, however, MAPK inhibition has cytostatic rather than cytotoxic effects, which may explain the lack of objective responses observed in early clinical trials of MEK inhibitors. Nevertheless, amenability of the MAPK pathway to pharmacodynamic evaluation and negligible clinical toxicity make MEK inhibitors an ideal platform to build pharmacological combinations with synergistic antitumor activity. In AML, the MEK/MAPK pathway is constitutively activated in the majority of cases (75%), conferring a uniformly poor prognosis; in preclinical models of AML, MEK blockade profoundly inhibits cell growth and proliferation and downregulates the expression of several anti-apoptotic players, thereby lowering the apoptotic threshold. Apoptosis induction, however, requires concentrations of MEK inhibitors much higher than those required to inhibit proliferation. Nevertheless, MEK blockade efficiently and selectively sensitizes leukemic cells to sub-optimal doses of other apoptotic stimuli, including classical cytotoxics (nucleoside analogs, microtubule-targeted drugs, ggr-irradiation), biologicals (retinoids, interferons, arsenic trioxide), and, most interestingly, other signal transduction/apoptosis modulators (UCN-01, STI571, Bcl-2 antagonists). In most instances, these MEK inhibition-based combinations result in a striking pro-apoptotic synergism in preclinical models. Here we briefly discuss evidence suggesting that MAPK pathway inhibition could play a prominent role in the development of integrated therapeutic strategies aimed at synergistic anti-leukemic effects. JF - Current Pharmaceutical Design AU - Milella, M AU - Precupanu, C M AU - Gregorj, C AU - Ricciardi, M R AU - Petrucci, M T AU - Kornblau, S M AU - Tafuri, A AU - Andreeff, M AD - Division of Medical Oncology A, Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2779 EP - 2795 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 11 IS - 21 SN - 1381-6128, 1381-6128 KW - Biotechnology and Bioengineering Abstracts KW - mapk KW - erk KW - leukemia KW - mek inhibitors KW - combination therapy KW - apoptosis KW - synergism KW - Cell survival KW - Apoptosis KW - Clinical trials KW - Tumor cells KW - Antagonists KW - Differentiation KW - Integration KW - Leukemia KW - Bcl-2 protein KW - Drugs KW - Pharmacodynamics KW - MAP kinase KW - Prognosis KW - Arsenic trioxide KW - Toxicity KW - Tumors KW - nucleoside analogs KW - Interferon KW - Cytotoxicity KW - MEK inhibitors KW - Retinoids KW - Cell proliferation KW - Antitumor activity KW - Signal transduction KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20281482?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Pharmaceutical+Design&rft.atitle=Beyond+Single+Pathway+Inhibition%3A+MEK+Inhibitors+as+a+Platform+for+the+Development+of+Pharmacological+Combinations+with+Synergistic+Anti-Leukemic+Effects&rft.au=Milella%2C+M%3BPrecupanu%2C+C+M%3BGregorj%2C+C%3BRicciardi%2C+M+R%3BPetrucci%2C+M+T%3BKornblau%2C+S+M%3BTafuri%2C+A%3BAndreeff%2C+M&rft.aulast=Milella&rft.aufirst=M&rft.date=2005-08-01&rft.volume=11&rft.issue=21&rft.spage=2779&rft.isbn=&rft.btitle=&rft.title=Current+Pharmaceutical+Design&rft.issn=13816128&rft_id=info:doi/10.2174%2F1381612054546842 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Cell survival; MAP kinase; Apoptosis; Prognosis; Arsenic trioxide; Tumors; Toxicity; Tumor cells; Clinical trials; Antagonists; nucleoside analogs; Leukemia; Integration; Differentiation; Interferon; Cytotoxicity; MEK inhibitors; Bcl-2 protein; Cell proliferation; Retinoids; Drugs; Pharmacodynamics; Signal transduction; Antitumor activity DO - http://dx.doi.org/10.2174/1381612054546842 ER - TY - JOUR T1 - New Monoclonal Antibodies to Mesothelin Useful for Immunohistochemistry, Fluorescence-Activated Cell Sorting, Western Blotting, and ELISA AN - 20237104; 6522348 AB - PURPOSE: Mesothelin is a cell surface protein that is highly expressed in some malignant tumors, and is a promising target for immunotherapy. Recent data suggests that mesothelin is an adhesive protein and may have a role in the metastases of ovarian cancer. Although a few monoclonal antibodies (MAb) to mesothelin have been produced, they have limitations for the study of expression of native mesothelin because of their low affinity or reactivity only with denatured mesothelin protein. We have produced novel MAbs to mesothelin to help study mesothelin function and to develop improved diagnosis and immunotherapy of mesothelin-expressing tumors. Experimental Design: Mesothelin-deficient mice were immunized with plasmid cDNA encoding mesothelin, and boosted with a mesothelin-rabbit IgG Fc fusion protein prior to cell fusion. Hybridomas were screened by an ELISA using plates coated with mesothelin-Fc protein. RESULTS: Seventeen hybridomas producing anti-mesothelin antibodies were established and shown to react with two epitopes on mesothelin. One group reacts with the same epitope as the low affinity antibody K1 that was originally used to identify mesothelin. The other is a new group that reacts with a new epitope. One antibody from each group was chosen for further study and shown to react strongly on ELISA, on immunohistochemistry, and by fluorescence-activated cell sorting on living cells. CONCLUSION: Our two newly established MAbs, MN and MB, have different and useful properties compared with current antibodies used for the detection of mesothelin by immunohistochemistry, fluorescence-activated cell sorting, ELISA, and Western blotting. JF - Clinical Cancer Research AU - Onda, Masanori AU - Willingham, Mark AU - Nagata, Satoshi AU - Bera, Tapan K AU - Beers, Richard AU - Ho, Mitchell AU - Hassan, Raffit AU - Kreitman, Robert J AU - Pastan, Ira AD - Authors' Affiliations: Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland and Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5840 EP - 5846 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 16 SN - 1078-0432, 1078-0432 KW - Biotechnology and Bioengineering Abstracts KW - Ovarian cancer KW - Western blotting KW - Fc KW - Cell surface KW - Enzyme-linked immunosorbent assay KW - Data processing KW - Monoclonal antibodies KW - Immunotherapy KW - Tumors KW - Plasmids KW - Cell fusion KW - Hybridoma KW - Flow cytometry KW - Metastases KW - Immunoglobulin G KW - Fusion protein KW - Adhesives KW - Manganese KW - Immunohistochemistry KW - Epitopes KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20237104?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=New+Monoclonal+Antibodies+to+Mesothelin+Useful+for+Immunohistochemistry%2C+Fluorescence-Activated+Cell+Sorting%2C+Western+Blotting%2C+and+ELISA&rft.au=Onda%2C+Masanori%3BWillingham%2C+Mark%3BNagata%2C+Satoshi%3BBera%2C+Tapan+K%3BBeers%2C+Richard%3BHo%2C+Mitchell%3BHassan%2C+Raffit%3BKreitman%2C+Robert+J%3BPastan%2C+Ira&rft.aulast=Onda&rft.aufirst=Masanori&rft.date=2005-08-01&rft.volume=11&rft.issue=16&rft.spage=5840&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Cell surface; Fc; Western blotting; Ovarian cancer; Enzyme-linked immunosorbent assay; Data processing; Monoclonal antibodies; Immunotherapy; Tumors; Plasmids; Cell fusion; Metastases; Flow cytometry; Hybridoma; Immunoglobulin G; Fusion protein; Adhesives; Immunohistochemistry; Manganese; Epitopes ER - TY - JOUR T1 - Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome AN - 20236866; 6703324 AB - The organization of eukaryotic genomes into distinct structural and functional domains is important for the regulation and transduction of genetic information. Here, we investigated heterochromatin and euchromatin profiles of the entire fission yeast genome and explored the role of RNA interference (RNAi) in genome organization. Histone H3 methylated at Lys4, which defines euchromatin, was not only distributed across most of the chromosomal landscape but was also present at the centromere core, the site of kinetochore assembly. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Notably, RNAi components were distributed throughout all these heterochromatin domains, and their localization depended on Clr4/Suv39h histone methyltransferase. Sequencing of small interfering RNAs (siRNAs) associated with the RITS RNAi effector complex identified hot spots of siRNAs, which mapped to a diverse array of elements in these RNAi-heterochromatin domains. We found that Clr4/Suv39h predominantly silenced repeat elements whose derived transcripts, transcribed mainly by RNA polymerase II, serve as a source for siRNAs. Our analyses also uncover an important role for the RNAi machinery in maintaining genomic integrity. JF - Nature Genetics AU - Cam, Hugh P AU - Sugiyama, Tomoyasu AU - Chen, Ee Sin AU - Chen, Xi AU - FitzGerald, Peter C AU - Grewal, Shiv I S AD - Laboratory of Molecular Cell Biology, National Institutes of Health, Bethesda, Maryland 20892-4255, USA., grewals@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 809 EP - 819 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 37 IS - 8 SN - 1061-4036, 1061-4036 KW - fission yeast KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology; Genetics Abstracts KW - Genomes KW - Euchromatin KW - Heterochromatin KW - Hot spots KW - Landscape KW - Centromeres KW - DNA-directed RNA polymerase KW - Islands KW - siRNA KW - Cores KW - epigenetics KW - Meiosis KW - Structure-function relationships KW - Kinetochores KW - histone methyltransferase KW - RNA-mediated interference KW - genomics KW - Histone H3 KW - Schizosaccharomyces pombe KW - K 03015:Fungi KW - G 07210:Cell cycle KW - W 30940:Products KW - N 14820:DNA Metabolism & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20236866?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Genetics&rft.atitle=Comprehensive+analysis+of+heterochromatin-+and+RNAi-mediated+epigenetic+control+of+the+fission+yeast+genome&rft.au=Cam%2C+Hugh+P%3BSugiyama%2C+Tomoyasu%3BChen%2C+Ee+Sin%3BChen%2C+Xi%3BFitzGerald%2C+Peter+C%3BGrewal%2C+Shiv+I+S&rft.aulast=Cam&rft.aufirst=Hugh&rft.date=2005-08-01&rft.volume=37&rft.issue=8&rft.spage=809&rft.isbn=&rft.btitle=&rft.title=Nature+Genetics&rft.issn=10614036&rft_id=info:doi/10.1038%2Fng1602 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Genomes; Euchromatin; Heterochromatin; Hot spots; Landscape; Centromeres; DNA-directed RNA polymerase; Islands; Cores; siRNA; Kinetochores; Structure-function relationships; Meiosis; epigenetics; histone methyltransferase; RNA-mediated interference; genomics; Histone H3; Schizosaccharomyces pombe DO - http://dx.doi.org/10.1038/ng1602 ER - TY - JOUR T1 - Methanococcus vannielii selenium-binding protein (SeBP): Chemical reactivity of recombinant SeBP produced in Escherichia coli AN - 20197475; 6519303 AB - A selenium-binding protein (SeBP) from Methanococcus vannielii was recently identified, and its gene was isolated and overexpressed in Escherichia coli [Self, W. T., Pierce, R. & Stadtman, T. C. (2004) IUBMB Life 56, 501-507]. SeBP and recombinant SeBP (rSeBP) migrated as approximately 42-kDa species on native gels and as approximately 33-kDa species on SDS gels. rSeBP consists of identical 8.8-kDa subunits, each containing a single cysteine residue. rSeBP isolated in the absence of reducing agents contained oxidized cysteine (89%) and very little bound selenium (0.05 eq or less per subunit). Complete reduction of the oxidized cysteine residues in rSeBP with Tris(2-carboxyethyl)phosphine required addition of a denaturant, such as 1 M guanidine-hydrochloride. With selenite as the selenium source and the isolated reduced protein as sole reductant, binding of one selenium per tetramer under anaerobic conditions required four cysteine thiol groups, one on each subunit. In the corresponding reaction, with reduced glutathione (GSH), equimolar amounts of selenodiglutathione (GSSeSG) and glutathione disulfide are formed from selenite and 4 GSH. At GSH-to-selenite ratios >4:1, conversion of GSSeSG to a perselenide derivative, GSSe super(-), occurs. However, with the reduced rSeBP as sole electron donor in the reaction with selenite, further conversion of the R-SSeS-R product apparently did not occur. Prior alkylation of the cysteine thiol groups in reduced rSeBP prevented selenite reduction and selenium binding under comparable conditions. JF - Proceedings of the National Academy of Sciences, USA AU - Patteson, Kemberly G AU - Trivedi, Neel AU - Stadtman, Thressa C AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012 Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 12029 EP - 12034 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 34 SN - 0027-8424, 0027-8424 KW - selenium-binding protein KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Oceanic Abstracts; Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Glutathione KW - selenite KW - Metabolites KW - Alkylation KW - Methanococcus vannielii KW - Protein structure KW - Gels KW - Recombinants KW - Selenium KW - Methanococcus KW - Anoxic conditions KW - Cysteine KW - Thiols KW - Reducing agents KW - Escherichia coli KW - Proteins KW - Coenzymes KW - Chemical modification KW - Q5 08503:Characteristics, behavior and fate KW - Q1 08421:Migrations and rhythms KW - G 07320:Bacterial genetics KW - O 4080:Pollution - Control and Prevention KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20197475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Methanococcus+vannielii+selenium-binding+protein+%28SeBP%29%3A+Chemical+reactivity+of+recombinant+SeBP+produced+in+Escherichia+coli&rft.au=Patteson%2C+Kemberly+G%3BTrivedi%2C+Neel%3BStadtman%2C+Thressa+C&rft.aulast=Patteson&rft.aufirst=Kemberly&rft.date=2005-08-01&rft.volume=102&rft.issue=34&rft.spage=12029&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Recombinants; Gels; Selenium; Anoxic conditions; Cysteine; Proteins; Metabolites; Coenzymes; Protein structure; Glutathione; Reducing agents; Thiols; selenite; Alkylation; Chemical modification; Methanococcus vannielii; Methanococcus; Escherichia coli ER - TY - JOUR T1 - A catalog for the transcripts from the venomous structures of the caterpillar Lonomia obliqua: Identification of the proteins potentially involved in the coagulation disorder and hemorrhagic syndrome AN - 20190806; 6501415 AB - Accidents with the caterpillar Lonomia obliqua are often associated with a coagulation disorder and hemorrhagic syndrome in humans. In the present study, we have constructed cDNA libraries from two venomous structures of the caterpillar, namely the tegument and the bristle. High-throughput sequencing and bioinformatics analyses were performed in parallel. Over one thousand cDNAs were obtained and clustered to produce a database of 538 contigs and singletons (clusters) for the tegument library and 368 for the bristle library. We have thus identified dozens of full-length cDNAs coding for proteins with sequence homology to snake venom prothrombin activator, trypsin-like enzymes, blood coagulation factors and prophenoloxidase cascade activators. We also report cDNA coding for cysteine proteases, Group III phospholipase A2, C-type lectins, lipocalins, in addition to protease inhibitors including serpins, Kazal-type inhibitors, cystatins and trypsin inhibitor-like molecules. Antibacterial proteins and housekeeping genes are also described. A significant number of sequences were devoid of database matches, suggesting that their biologic function remains to be defined. We also report the N-terminus of the most abundant proteins present in the bristle, tegument, hemolymph, and ''cryosecretion''. Thus, we have created a catalog that contains the predicted molecular weight, isoelectric point, accession number, and putative function for each selected molecule from the venomous structures of L. obliqua. The role of these molecules in the coagulation disorder and hemorrhagic syndrome caused by envenomation with this caterpillar is discussed. All sequence information and the Supplemental Data, including figures and tables with hyperlinks to FASTA-formatted files for each contig and the best match to the databases, are available at http:/ /www.ncbi.nih.gov/projects/omes. JF - Gene AU - Veiga, ABG AU - Ribeiro, JMC AU - Guimaraes, JA AU - Francischetti, IMB AD - Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, 12735 Twinbrook Parkway, Twinbrook III, Room 2E-28, Rockville, MD 20892-8132, USA, ifrancischetti@niaid.nih.gov Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 11 EP - 27 PB - Elsevier B.V. VL - 355 SN - 0378-1119, 0378-1119 KW - Microbiology Abstracts B: Bacteriology; Entomology Abstracts; Genetics Abstracts KW - Bristles KW - Catalogs KW - Hemorrhage KW - N-Terminus KW - Lonomia obliqua KW - Accidents KW - Molecular weight KW - prophenoloxidase KW - Lipocalin KW - serpins KW - Cysteine proteinase KW - Hemolymph KW - Isoelectric points KW - Data processing KW - Tegument KW - Trypsin KW - Phospholipase A2 KW - Proteinase inhibitors KW - Enzymes KW - Lectins KW - Cystatin KW - Databases KW - Blood coagulation KW - prothrombin KW - Homology KW - Bioinformatics KW - Venom KW - G 07480:Hematological disorders KW - Z 05320:Physiology, Anatomy, and Biochemistry KW - J 02340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20190806?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene&rft.atitle=A+catalog+for+the+transcripts+from+the+venomous+structures+of+the+caterpillar+Lonomia+obliqua%3A+Identification+of+the+proteins+potentially+involved+in+the+coagulation+disorder+and+hemorrhagic+syndrome&rft.au=Veiga%2C+ABG%3BRibeiro%2C+JMC%3BGuimaraes%2C+JA%3BFrancischetti%2C+IMB&rft.aulast=Veiga&rft.aufirst=ABG&rft.date=2005-08-01&rft.volume=355&rft.issue=&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Gene&rft.issn=03781119&rft_id=info:doi/10.1016%2Fj.gene.2005.05.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Bristles; Catalogs; Hemorrhage; N-Terminus; Accidents; Molecular weight; prophenoloxidase; Lipocalin; serpins; Cysteine proteinase; Hemolymph; Isoelectric points; Data processing; Trypsin; Tegument; Phospholipase A2; Proteinase inhibitors; Enzymes; Lectins; Databases; Cystatin; Blood coagulation; prothrombin; Homology; Bioinformatics; Venom; Lonomia obliqua DO - http://dx.doi.org/10.1016/j.gene.2005.05.002 ER - TY - JOUR T1 - Crystal Structure of the Bacterial YhcH Protein Indicates a Role in Sialic Acid Catabolism AN - 20152103; 6517733 AB - The yhcH gene is part of the nan operon in bacteria that encodes proteins involved in sialic acid catabolism. Determination of the crystal structure of YhcH from Haemophilus influenzae was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. The structure was determined at 2.2-Aa resolution by multiple-wavelength anomalous diffraction. The protein fold is a variation of the double-stranded beta -helix. Two antiparallel beta -sheets form a funnel opened at one side, where a putative active site contains a copper ion coordinated to the side chains of two histidine and two carboxylic acid residues. A comparison to other proteins with a similar fold and analysis of the genomic context suggested that YhcH may be a sugar isomerase involved in processing of exogenous sialic acid. JF - Journal of Bacteriology AU - Teplyakov, Alexey AU - Obmolova, Galina AU - Toedt, John AU - Galperin, Michael Y AU - Gilliland, Gary L AD - Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute and National Institute of Standards and Technology, Rockville, Maryland. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5520 EP - 5527 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - YhcH protein KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Bacteria KW - Sugar KW - Haemophilus influenzae KW - Copper KW - Protein folding KW - Histidine KW - Crystal structure KW - carboxylic acids KW - genomics KW - Diffraction KW - Operons KW - Sialic acids KW - G 07320:Bacterial genetics KW - A 01490:Miscellaneous KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20152103?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Crystal+Structure+of+the+Bacterial+YhcH+Protein+Indicates+a+Role+in+Sialic+Acid+Catabolism&rft.au=Teplyakov%2C+Alexey%3BObmolova%2C+Galina%3BToedt%2C+John%3BGalperin%2C+Michael+Y%3BGilliland%2C+Gary+L&rft.aulast=Teplyakov&rft.aufirst=Alexey&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5520&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Sugar; Protein folding; Histidine; carboxylic acids; Crystal structure; Copper; Diffraction; genomics; Operons; Sialic acids; Bacteria; Haemophilus influenzae ER - TY - JOUR T1 - The efficacy of mesenchymal stem cells to regenerate and repair dental structures AN - 19991725; 6627819 AB - Authors -: Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S Objectives -: Identification, characterization, and potential application of mesenchymal stem cells (MSC) derived from human dental tissues. Methods -: Dental pulp and periodontal ligament were obtained from normal human impacted third molars. The tissues were digested in collagenase/dispase to generate single cell suspensions. Cells were cultured in alpha -MEM supplemented with 20% fetal bovine serum, 2 mM l-glutamine, 100 mu M l-ascorbate-2-phosphate. Magnetic and fluorescence activated cell sorting were employed to characterize the phenotype of freshly isolated and ex vivo expanded cell populations. The developmental potential of cultured cells was assessed following co-transplantation with hydroxyapetite/tricalcium phosphate (HA/TCP) particles into immunocompromised mice for 8 weeks. Results -: MSC were identified in adult human dental pulp (dental pulp stem cells, DPSC), human primary teeth (stem cells from human exfoliated deciduous teeth, SHED), and periodontal ligament (periodontal ligament stem cells, PDLSC) by their capacity to generate clongenic cell clusters in culture. Ex vivo expanded DPSC, SHED, and PDLSC populations expressed a heterogeneous assortment of makers associated with MSC, dentin, bone, smooth muscle, neural tissue, and endothelium. PDLSC were also found to express the tendon specific marker, Scleraxis. Xenogeneic transplants containing HA/TCP with either DPSC or SHED generated donor-derived dentin-pulp-like tissues with distinct odontoblast layers lining the mineralized dentin-matrix. In parallel studies, PDLSC generated cementum-like structures associated with PDL-like connective tissue when transplanted with HA/TCP into immunocompromised mice. Conclusion -: Collectively, these data revealed the presence of distinct MSC populations associated with dental structures with the potential of stem cells to regenerate living human dental tissues in vivo. JF - Orthodontics and Craniofacial Research AU - Shi, S AU - Bartold, P M AU - Miura, M AU - Seo, B M AU - Robey, P G AU - Gronthos, S AD - S. Shi, M. Miura, B.M. Seo, P.G. Robey, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, MD, USA P.M. Bartold, Colgate Australian Clinical Dental Research Centre, Dental School, University of Adelaide, Adelaide Dental Hospital, Adelaide, SA, Australia S. Gronthos, Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, SA, Australia, stan.gronthos@imvs.sa.gov.au Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 191 EP - 199 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 8 IS - 3 SN - 1601-6335, 1601-6335 KW - Biotechnology and Bioengineering Abstracts; Calcium & Calcified Tissue Abstracts KW - Odontoblasts KW - Teeth KW - Smooth muscle KW - Cell suspensions KW - Dentin KW - Glutamine KW - Data processing KW - Collagenase KW - Connective tissues KW - Molars KW - Cell culture KW - Bone KW - Flow cytometry KW - Stem cells KW - periodontal ligament KW - Endothelium KW - Mesenchyme KW - tricalcium phosphate KW - Tendons KW - Dental pulp KW - T 2045:Teeth KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19991725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Orthodontics+and+Craniofacial+Research&rft.atitle=The+efficacy+of+mesenchymal+stem+cells+to+regenerate+and+repair+dental+structures&rft.au=Shi%2C+S%3BBartold%2C+P+M%3BMiura%2C+M%3BSeo%2C+B+M%3BRobey%2C+P+G%3BGronthos%2C+S&rft.aulast=Shi&rft.aufirst=S&rft.date=2005-08-01&rft.volume=8&rft.issue=3&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Orthodontics+and+Craniofacial+Research&rft.issn=16016335&rft_id=info:doi/10.1111%2Fj.1601-6343.2005.00331.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - SuppNotes - Figures, 1; tables, 1. N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Cell suspensions; Smooth muscle; Teeth; Odontoblasts; Dentin; Glutamine; Data processing; Molars; Connective tissues; Collagenase; Cell culture; Flow cytometry; Bone; Stem cells; periodontal ligament; Endothelium; Mesenchyme; Tendons; tricalcium phosphate; Dental pulp DO - http://dx.doi.org/10.1111/j.1601-6343.2005.00331.x ER - TY - JOUR T1 - Toward a live microbial microbicide for HIV: Commensal bacteria secreting an HIV fusion inhibitor peptide AN - 19936109; 6519297 AB - Most HIV transmission occurs on the mucosal surfaces of the gastrointestinal and cervicovaginal tracts, both of which are normally coated by a biofilm of nonpathogenic commensal bacteria. We propose to genetically engineer such naturally occurring bacteria to protect against HIV infection by secreting antiviral peptides. Here we describe the development and characterization of Nissle 1917, a highly colonizing probiotic strain of Escherichia coli, secreting HIV-gp41-hemolysin A hybrid peptides that block HIV fusion and entry into target cells. By using an appropriate combination of cis- and transacting secretory and regulatory signals, micromolar secretion levels of the anti-HIV peptides were achieved. The genetically engineered Nissle 1917 were capable of colonizing mice for periods of weeks to months, predominantly in the colon and cecum, with lower concentrations of bacteria present in the rectum, vagina, and small intestine. Histological and immunocytochemical examination of the colon revealed bacterial growth and peptide secretion throughout the luminal mucosa and in association with epithelial surfaces. The use of genetically engineered live microbes as anti-HIV microbicides has important potential advantages in economy, efficacy, and durability. JF - Proceedings of the National Academy of Sciences, USA AU - Rao, Srinivas AU - Hu, Stella AU - McHugh, Louise AU - Lueders, Kira AU - Henry, Ken AU - Zhao, Qi AU - Fekete, Richard A AU - Kar, Sudeshna AU - Adhya, Sankar AU - Hamer, Dean H AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Laboratories of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 11993 EP - 11998 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 34 SN - 0027-8424, 0027-8424 KW - HIV KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Genetics Abstracts; Biotechnology and Bioengineering Abstracts; Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology; Medical and Pharmaceutical Biotechnology Abstracts KW - Rectum KW - glycoprotein gp41 KW - Mucosa KW - probiotics KW - Commensals KW - Small intestine KW - Infection KW - Disease transmission KW - Antiviral agents KW - Colon KW - Human immunodeficiency virus KW - Genetic engineering KW - Hybrids KW - Vagina KW - Escherichia coli KW - Cecum KW - Biofilms KW - microbicides KW - A 01340:Antibiotics & Antimicrobials KW - J 02732:Other cell constituents and metabolites KW - V 22002:AIDS: Molecular and in vitro aspects KW - W3 33372:Antiviral agents KW - W 30965:Miscellaneous, Reviews KW - G 07770:Bacteria KW - J 02740:Genetics and evolution KW - J 02812:Antibacterial Agents: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19936109?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.genre=dissertations+%26+theses&rft.jtitle=&rft.atitle=&rft.au=Ayvazian%2C+John&rft.aulast=Ayvazian&rft.aufirst=John&rft.date=1996-09-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Parenting+styles+in+the+Armenian+family&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Rectum; glycoprotein gp41; Mucosa; Commensals; probiotics; Small intestine; Infection; Disease transmission; Colon; Antiviral agents; Hybrids; Genetic engineering; Vagina; Cecum; Biofilms; microbicides; Human immunodeficiency virus; Escherichia coli ER - TY - JOUR T1 - An anti-HIV microbicide comes alive AN - 19934422; 6519350 AB - Topical microbicides are a broad class of agents designed to block or kill infectious microorganisms directly at the site of transmission. With the AIDS pandemic continuing its unrelenting global march (40 million current infections, 14,000 new infections per day) driven largely by sexual transmission, microbicides have moved steadily toward the front line of preventative strategies. Indeed, many candidate anti-HIV microbicides are currently under development, with several already in clinical trials. A battery of promising protein-based HIV inhibitors can potentially be developed, but they face serious challenges of high production costs and instability during transport and storage. In a recent issue of PNAS, Rao et al. presented an intriguing version of a "live microbicide" approach whereby a commensal bacterium is engineered to secrete a potent anti-HIV peptide. When administered orally or as a rectal suppository, the bacteria would colonize the gut mucosa and secrete the peptide in situ, thereby providing protection in advance of exposure hopefully for days, weeks, or even months. This delivery mode would be highly advantageous over others requiring repeated topical application before each act of intercourse; also, the engineered bacteria would be relatively simple and inexpensive to manufacture, transport, and store. JF - Proceedings of the National Academy of Sciences, USA AU - Lagenaur, Laurel A AU - Berger, Edward A AD - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 12294 EP - 12295 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 35 SN - 0027-8424, 0027-8424 KW - HIV KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Virology & AIDS Abstracts KW - Acquired immune deficiency syndrome KW - Rectum KW - Mucosa KW - Commensals KW - Infection KW - Clinical trials KW - Topical application KW - Disease transmission KW - pandemics KW - Digestive tract KW - Human immunodeficiency virus KW - Microorganisms KW - microbicides KW - V 22002:AIDS: Molecular and in vitro aspects KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19934422?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologya&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=An+anti-HIV+microbicide+comes+alive&rft.au=Lagenaur%2C+Laurel+A%3BBerger%2C+Edward+A&rft.aulast=Lagenaur&rft.aufirst=Laurel&rft.date=2005-08-01&rft.volume=102&rft.issue=35&rft.spage=12294&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - pandemics; Acquired immune deficiency syndrome; Rectum; Digestive tract; Mucosa; Microorganisms; Commensals; Infection; Clinical trials; Disease transmission; Topical application; microbicides; Human immunodeficiency virus ER - TY - JOUR T1 - A quantitative determination of multi-protein interactions by the analysis of confocal images using a pixel-by-pixel assessment algorithm AN - 19808756; 6480790 AB - MOTIVATION: Recent advances in confocal microscopy have allowed scientists to assess the expression, and to some extent, the interaction/colocalization of multiple molecules within cells and tissues. In some instances, accurately quantifying the colocalization of two or more proteins may be critical. This can require the acquisition of multiple Z plane images (Z stacks) throughout a specimen and, as such, we report here the successful development of a freeware, open-source image analysis tool, IMAJIN_COLOC, developed in PERL (v. 5.8, build 806), using the PERLMagick libraries (ImageMagick). Using a pixel-by-pixel analysis algorithm, IMAJIN_COLOC can analyze images for antigen expression (any number of colors) and can measure all possible combinations of colocalization for up to three colors by analyzing a Z stack gallery acquired for each sample. The simultaneous (i.e. in a single pass) analysis of three-color colocalization, and batch analysis capabilities are distinctive features of this program. RESULTS: A control image, containing known individual and colocalized pixel counts, was used to validate the accuracy of IMAJIN_COLOC. As further validation, pixel counts and colocalization values from the control image were compared to those obtained with the software packaged with the Zeiss laser-scanning microscope (LSM AIM, version 3.2). The values from both programs were found to be identical. To demonstrate the applicability of this program in addressing novel biological questions, we examined the role of neurons in eliciting an immune reaction in response to viral infection. Specifically, we successfully examined expression of the chemokine RANTES in measles virus (MV) infected hippocampal neurons and quantified changes in RANTES production throughout the disease period. The resultant quantitative data were also evaluated visually, using a gif image created during the analysis. AVAILABILITY: PERL (ActivePerl, version 5.8) is available at activestate.com; the PERLMagick libraries are available at imagemagick.org, and IMAJIN_COLOC, the source code and user documentation can be downloaded from http://www.fda.gov/cber/research/imaging/imageanalysis.htm JF - Bioinformatics AU - Goucher AU - Wincovitch, S M AU - Garfield, SH AU - Carbone, K M AU - Malik, TH AD - DVP/OVRR, Center for Biologics Evaluation and Research, US Food and Drug Administration Bethesda, MD 20892, USA. Laboratory of Experimental Carcinogenesis, National Cancer Institute Bethesda, MD 20892, USA. Department of Psychiatry and Department of Medicine, Johns Hopkins University Baltimore, MD, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 3248 EP - 3254 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 15 SN - 1367-4803, 1367-4803 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts KW - Galleries KW - Chemokines KW - Data processing KW - Hippocampus KW - Microscopes KW - Algorithms KW - RANTES KW - Image processing KW - Measles virus KW - Infection KW - Color KW - Computer programs KW - software KW - Neurons KW - Confocal microscopy KW - Bioinformatics KW - V 22320:Replication KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19808756?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=The+News+Tribune&rft.atitle=Book+ban+appeal+goes+to+FWay+board+%27Catcher+in+the+Rye%27%3A+Jefferson+High+parent+protests+often-+challenged+novel%3A+%5BSouth+Sound+Edition%5D&rft.au=Robinson%2C+Sean&rft.aulast=Robinson&rft.aufirst=Sean&rft.date=2001-07-17&rft.volume=&rft.issue=&rft.spage=B.1&rft.isbn=&rft.btitle=&rft.title=The+News+Tribune&rft.issn=10735860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Galleries; Chemokines; Data processing; Hippocampus; Microscopes; Algorithms; Image processing; RANTES; Infection; Color; Computer programs; software; Neurons; Confocal microscopy; Bioinformatics; Measles virus ER - TY - JOUR T1 - Escherichia coli K1's Capsule Is a Barrier to Bacteriophage T7 AN - 19443084; 6520443 AB - Escherichia coli strains that produce the K1 polysaccharide capsule have long been associated with pathogenesis. This capsule is believed to increase the cell's invasiveness, allowing the bacteria to avoid phagocytosis and inactivation by complement. It is also recognized as a receptor by some phages, such as K1F and K1-5, which have virion-associated enzymes that degrade the polysaccharide. In this report we show that expression of the K1 capsule in E. coli physically blocks infection by T7, a phage that recognizes lipopolysaccharide as the primary receptor. Enzymatic removal of the K1 antigen from the cell allows T7 to adsorb and replicate. This observation suggests that the capsule plays an important role as a defense against some phages that recognize structures beneath it and that the K1-specific phages evolved to counter this physical barrier. JF - Applied and Environmental Microbiology AU - Scholl, Dean AU - Adhya, Sankar AU - Merril, Carl AD - Section of Biochemical Genetics, The National Institute of Mental Health, NIH, Building 36, Room 3D20, 9000 Rockville Pike, Bethesda, Maryland 20892. Section of Developmental Genetics, The National Cancer Institute, NIH, Building 37, Room 5138C, 9000 Rockville Pike, Bethesda, Maryland 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4872 EP - 4874 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 71 IS - 8 SN - 0099-2240, 0099-2240 KW - Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - Phages KW - Capsules KW - Invasiveness KW - Barriers KW - Complement KW - Escherichia coli KW - Lipopolysaccharides KW - Polysaccharides KW - Infection KW - Phagocytosis KW - J 02750:Phage-host interactions KW - V 22070:Phage-host interactions including lysogeny & transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19443084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=Fort+Worth+Star+-+Telegram&rft.atitle=%27Rye%27+relevance+50+years+ago%2C+J.D.+Salinger+put+a+face+on+teen+angst+and+gave+it+credibility.+Since+then%2C+his+%27Catcher+in+the+Rye%27+has+become+a+multimedia+mega-force%2C+influencing+books%2C+movies%2C+music+and+television.%3A+%5BFINAL+Edition%5D&rft.au=Guinn%2C+Jeff&rft.aulast=Guinn&rft.aufirst=Jeff&rft.date=2001-08-05&rft.volume=&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Fort+Worth+Star+-+Telegram&rft.issn=08890013&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Phages; Capsules; Invasiveness; Barriers; Complement; Lipopolysaccharides; Phagocytosis; Infection; Polysaccharides; Escherichia coli ER - TY - JOUR T1 - Prediction error estimation: a comparison of resampling methods AN - 19427956; 6480797 AB - MOTIVATION: In genomic studies, thousands of features are collected on relatively few samples. One of the goals of these studies is to build classifiers to predict the outcome of future observations. There are three inherent steps to this process: feature selection, model selection and prediction assessment. With a focus on prediction assessment, we compare several methods for estimating the 'true' prediction error of a prediction model in the presence of feature selection. RESULTS: For small studies where features are selected from thousands of candidates, the resubstitution and simple split-sample estimates are seriously biased. In these small samples, leave-one-out cross-validation (LOOCV), 10-fold cross-validation (CV) and the .632+ bootstrap have the smallest bias for diagonal discriminant analysis, nearest neighbor and classification trees. LOOCV and 10-fold CV have the smallest bias for linear discriminant analysis. Additionally, LOOCV, 5- and 10-fold CV, and the .632+ bootstrap have the lowest mean square error. The .632+ bootstrap is quite biased in small sample sizes with strong signal-to-noise ratios. Differences in performance among resampling methods are reduced as the number of specimens available increase. CONTACT: annette.molinaroale.edu Supplementary Information: A complete compilation of results and R code for simulations and analyses are available in Molinaro et al. (2005) (http://linus.nci.nih.gov/brb/TechReport.htm). JF - Bioinformatics AU - Molinaro, Annette M AU - Simon, Richard AU - Pfeiffer, Ruth M AD - Biostatistics Branch, Division of Cancer Epidemiology and Genetics, NCI, NIH Rockville, MD 20852 USA. Biometric Research Branch, Division of Cancer Treatment and Diagnostics, NCI, NIH Rockville, MD 20852 USA. Department of Epidemiology and Public Health, Yale University School of Medicine New Haven, CT 06520, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 3301 EP - 3307 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 15 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts KW - Bioinformatics KW - genomics KW - Models KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19427956?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Prediction+error+estimation%3A+a+comparison+of+resampling+methods&rft.au=Molinaro%2C+Annette+M%3BSimon%2C+Richard%3BPfeiffer%2C+Ruth+M&rft.aulast=Molinaro&rft.aufirst=Annette&rft.date=2005-08-01&rft.volume=21&rft.issue=15&rft.spage=3301&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Models; genomics; Bioinformatics ER - TY - JOUR T1 - Prostate Cancer: Correlation of MR Imaging and MR Spectroscopy with Pathologic Findings after Radiation Therapy-Initial Experience AN - 19420999; 6477695 AB - PURPOSE: To prospectively evaluate magnetic resonance (MR) imaging and MR spectroscopy for depiction of local prostate cancer recurrence after external-beam radiation therapy, with step-section pathologic findings as the standard of reference. MATERIALS AND METHODS: Study received institutional approval, and written informed consent was obtained. Study was compliant with Health Insurance Portability and Accountability Act. Sextant biopsy, digital rectal examination, MR imaging, MR spectroscopy, and salvage radical prostatectomy with step-section pathologic examination were performed in nine patients with increasing prostate-specific antigen levels after external-beam radiation therapy. MR imaging criterion for tumor was a focal nodular region of reduced signal intensity at T2-weighted imaging. MR spectroscopic criteria for tumor were voxels with choline (Cho) plus creatine (Cr) to citrate (Cit) ratio ([Cho + Cr]/Cit) of at least 0.5 or voxels with detectable Cho and no Cit in the peripheral zone. Sensitivity and specificity of sextant biopsy, digital rectal examination, MR imaging, and MR spectroscopy were determined by using a prostate sextant as the unit of analysis. For feature analysis, MR imaging and MR spectroscopic findings were correlated with step-section pathologic findings. RESULTS: MR imaging and MR spectroscopy showed estimated sensitivities of 68% and 77%, respectively, while sensitivities of biopsy and digital rectal examination were 48% and 16%, respectively. MR spectroscopy appears to be less specific (78%) than the other three tests, each of which had a specificity higher than 90%. MR spectroscopic feature analysis showed that a metabolically altered benign gland could be falsely identified as tumor by using MR spectroscopic criteria; further analysis of MR spectroscopic features did not lead to improved MR spectroscopic criteria for recurrent tumor. CONCLUSION: In summary, MR imaging and MR spectroscopy may be more sensitive than sextant biopsy and digital rectal examination for sextant localization of cancer recurrence after external-beam radiation therapy. [copy ] RSNA, 2005 JF - Radiology AU - Pucar, Darko AU - Shukla-Dave, Amita AU - Hricak, Hedvig AU - Moskowitz, Chaya S AU - Kuroiwa, Kentaro AU - Olgac, Semra AU - Ebora, Lanie E AU - Scardino, Peter T AU - Koutcher, Jason A AU - Zakian, Kristen L AD - Departments of Radiology (D.P., A.S.D., H.H., L.E., J.A.K., K.L.Z.), Medical Physics (D.P., A.S.D., L.E., J.A.K., K.L.Z.), Epidemiology and Biostatistics (C.M.), Urology (K.K., P.T.S.), Pathology (K.K., S.O.), and Medicine (J.A.K.), Memorial Sloan-Kettering Cancer Center, 1275 York Ave, New York, NY 10021. Supported by National Institutes of Health grant R01 CA76423 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 545 EP - 553 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 236 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts KW - Choline KW - Rectum KW - Magnetic resonance imaging KW - Creatine KW - Biopsy KW - Tumors KW - prostate-specific antigen KW - Cancer KW - Prostate cancer KW - Radiation KW - Glands KW - Magnetic resonance spectroscopy KW - Radicals KW - Citric acid KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420999?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Prostate+Cancer%3A+Correlation+of+MR+Imaging+and+MR+Spectroscopy+with+Pathologic+Findings+after+Radiation+Therapy-Initial+Experience&rft.au=Pucar%2C+Darko%3BShukla-Dave%2C+Amita%3BHricak%2C+Hedvig%3BMoskowitz%2C+Chaya+S%3BKuroiwa%2C+Kentaro%3BOlgac%2C+Semra%3BEbora%2C+Lanie+E%3BScardino%2C+Peter+T%3BKoutcher%2C+Jason+A%3BZakian%2C+Kristen+L&rft.aulast=Pucar&rft.aufirst=Darko&rft.date=2005-08-01&rft.volume=236&rft.issue=2&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Magnetic resonance imaging; Magnetic resonance spectroscopy; Biopsy; Radiation; Rectum; Tumors; Prostate cancer; Glands; Citric acid; Creatine; Radicals; prostate-specific antigen; Cancer; Choline ER - TY - JOUR T1 - BioMart and Bioconductor: a powerful link between biological databases and microarray data analysis AN - 19420375; 6522682 AB - SUMMARY: biomaRt is a new Bioconductor package that integrates BioMart data resources with data analysis software in Bioconductor. It can annotate a wide range of gene or gene product identifiers (e.g. Entrez-Gene and Affymetrix probe identifiers) with information such as gene symbol, chromosomal coordinates, Gene Ontology and OMIM annotation. Furthermore biomaRt enables retrieval of genomic sequences and single nucleotide polymorphism information, which can be used in data analysis. Fast and up-to-date data retrieval is possible as the package executes direct SQL queries to the BioMart databases (e.g. Ensembl). The biomaRt package provides a tight integration of large, public or locally installed BioMart databases with data analysis in Bioconductor creating a powerful environment for biological data mining. AVAILABILITY: http://www.bioconductor.org. LGPL CONTACT: steffen.durincksat.kuleuven.ac.be JF - Bioinformatics AU - Durinck, Steffen AU - Moreau, Yves AU - Kasprzyk, Arek AU - Davis, Sean AU - De Moor, Bart AU - Brazma, Alvis AU - Huber, Wolfgang AD - Department of Electronical Engineering ESAT-SCD, K.U.Leuven, Kasteelpark Arenberg 10, 3001 Leuven-Heverlee, Belgium. EBI, Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SD, UK. Cancer Genetics Branch, National Human Genome Research Institute, National Institute of Health 50 South Drive, Bethesda, MD 20892-8000, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3439 EP - 3440 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 16 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts KW - Integration KW - Computer programs KW - Databases KW - software KW - Data processing KW - Single-nucleotide polymorphism KW - DNA probes KW - genomics KW - Bioinformatics KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420375?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=BioMart+and+Bioconductor%3A+a+powerful+link+between+biological+databases+and+microarray+data+analysis&rft.au=Durinck%2C+Steffen%3BMoreau%2C+Yves%3BKasprzyk%2C+Arek%3BDavis%2C+Sean%3BDe+Moor%2C+Bart%3BBrazma%2C+Alvis%3BHuber%2C+Wolfgang&rft.aulast=Durinck&rft.aufirst=Steffen&rft.date=2005-08-01&rft.volume=21&rft.issue=16&rft.spage=3439&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Data processing; Databases; Bioinformatics; genomics; Computer programs; software; Integration; Single-nucleotide polymorphism; DNA probes ER - TY - JOUR T1 - Optimizing brain tissue contrast with EPI: A simulated annealing approach AN - 19418625; 6489828 AB - A new magnetization preparation and image acquisition scheme was developed to obtain high-resolution brain images with optimal tissue contrast. The pulse sequence was derived from an optimization process using simulated annealing, without prior assumptions with regard to the number of radiofrequency (RF) pulses and flip angles. The resulting scheme combined two inversion pulses with the acquisition of three images with varying contrast. The combination of the three images allowed separation of gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) based on T sub(1), contrast. It also enabled the correction of small errors in the initial T sub(1) estimates in postprocessing. The use of three-dimensional (3D) sensitivity-encoded (SENSE) echo-planar imaging (EPI) for image acquisition made it possible to achieve a 1.15 super(3) mm super(3) isotropic resolution within a scan time of 10 min 21 s. The cortical GM signal-to-noise ratio (SNR) in the calculated GM-only image varied between 30 and 100. The novel technique was evaluated in combination with blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI) on human subjects, and provided for excellent coregistration of anatomical and functional data. JF - Magnetic Resonance in Medicine AU - Ikonomidou, Vasiliki N AU - Van Gelderen, Peter AU - De Zwart, Jacco A AU - Fukunaga, Masaki AU - Duyn, Jeff H AD - Advanced MRI Section, LFMI, NINDS, National Institutes of Health, Bldg. 10, Rm. B1D-722, MSC 1065, 9000 Rockville Pike, Bethesda, MD 20892-1065, USA, viko@nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 373 EP - 385 PB - John Wiley & Sons, Ltd. VL - 54 IS - 2 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Cerebrospinal fluid KW - Inversion KW - Functional magnetic resonance imaging KW - Brain KW - Substantia alba KW - N.M.R. KW - Substantia grisea KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19418625?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Optimizing+brain+tissue+contrast+with+EPI%3A+A+simulated+annealing+approach&rft.au=Ikonomidou%2C+Vasiliki+N%3BVan+Gelderen%2C+Peter%3BDe+Zwart%2C+Jacco+A%3BFukunaga%2C+Masaki%3BDuyn%2C+Jeff+H&rft.aulast=Ikonomidou&rft.aufirst=Vasiliki&rft.date=2005-08-01&rft.volume=54&rft.issue=2&rft.spage=373&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20561 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Functional magnetic resonance imaging; Cerebrospinal fluid; Brain; Neuroimaging; N.M.R.; Substantia alba; Inversion; Substantia grisea DO - http://dx.doi.org/10.1002/mrm.20561 ER - TY - JOUR T1 - Early Immune Response to the Components of the Type III System of Pseudomonas aeruginosa in Children with Cystic Fibrosis AN - 17665566; 6520123 AB - The lungs of patients with cystic fibrosis (CF) are colonized initially by Pseudomonas aeruginosa, which is associated with progressive lung destruction and increased mortality. The pathogenicity of P. aeruginosa is caused by a number of virulence factors, including exotoxin A (ETA) and the type III cytotoxins (ExoS, ExoT, ExoU, and ExoY). P. aeruginosa contacts the plasma membrane to deliver type III cytotoxins through a channel formed by PopB, PopD, and PcrV; ETA enters mammalian cells via receptor-mediated endocytosis. The Wisconsin CF Neonatal Screening Project is a longitudinal investigation to assess the potential benefits and risks of newborn screening for CF; the project was the source of serum samples used in this study. Past studies evaluated the longitudinal appearance of antibodies to ETA and elastase and P. aeruginosa infections in patients with CF. The current study characterized the longitudinal appearance of antibodies to components of the type III system in children with CF. Western blot analyses showed that serum antibodies to PopB, PcrV, and ExoS were common. Longitudinal enzyme-linked immunosorbent assays determined that the first detection of antibodies to pooled ExoS/PopB occurred at a time similar to those of detection of antibodies to a P. aeruginosa cell lysate and the identification of oropharyngeal cultures positive for P. aeruginosa. This indicates that children with CF are colonized early with P. aeruginosa expressing the type III system, implicating it in early pathogenesis, and implies that surveillance of clinical symptoms, oropharyngeal cultures, and seroconversion to type III antigens may facilitate early detection of P. aeruginosa infections. JF - Journal of Clinical Microbiology AU - Corech, R AU - Rao, A AU - Laxova, A AU - Moss, J AU - Rock, MJ AU - Li, Z AU - Kosorok, M R AU - Splaingard, M L AU - Farrell, P M AU - Barbieri, J T AD - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin. Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin. Department of Pediatrics, University of Wisconsin Medical School, Madison, Wisconsin. Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland. Department of Biostatistics and Medical Informatics, University of Wisconsin Medical School, Madison, Wisconsin Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3956 EP - 3962 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 8 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17665566?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Behavioral+Development&rft.atitle=Bidirectional+relations+between+temperament+and+parenting+styles+in+chinese+children&rft.au=Lee%2C+Erica+H.%3BZhou%2C+Qing%3BEisenberg%2C+Nancy%3BWang%2C+Yun&rft.aulast=Lee&rft.aufirst=Erica&rft.date=2013-01-01&rft.volume=37&rft.issue=1&rft.spage=57&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Behavioral+Development&rft.issn=01650254&rft_id=info:doi/10.1177%2F0165025412460795 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Phylogenetic Analysis of the Spirochetes Borrelia parkeri and Borrelia turicatae and the Potential for Tick-Borne Relapsing Fever in Florida AN - 17663669; 6520109 AB - Isolates of Borrelia turicatae, Borrelia parkeri, and the Florida canine borrelia (FCB) were examined to further phylogenetically characterize the identities of these spirochetes in the United States. DNA sequences of four chromosomal loci (the 16S rRNA gene, flaB, gyrB, and glpQ) were determined for eight isolates of B. turicatae and six isolates of B. parkeri, which grouped the spirochetes into two distinct but closely related taxa (>98% sequence identity) separate from Borrelia hermsii. The FCB was clearly separated with the group identified as B. turicatae, confirming this bacterium as a relapsing fever spirochete. Therefore, the potential for tick-borne relapsing fever in humans and other animals exists in Florida and future efforts are needed to determine the enzootic hosts and distribution of this spirochete in the southeastern United States. Analysis of plasmids demonstrated both linear and circular forms in B. turicatae but only linear plasmids in B. parkeri, which should be of interest to investigators concerned with plasmid diversity and evolution within this group of spirochetes. JF - Journal of Clinical Microbiology AU - Schwan, Tom G AU - Raffel, Sandra J AU - Schrumpf, Merry E AU - Policastro, Paul F AU - Rawlings, Julie A AU - Lane, Robert S AU - Breitschwerdt, Edward B AU - Porcella, Stephen F AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana. Community Preparedness Section, Texas Department of State Health Services, Austin, Texas. Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California. Department of Companion and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3851 EP - 3859 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 8 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17663669?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Phylogenetic+Analysis+of+the+Spirochetes+Borrelia+parkeri+and+Borrelia+turicatae+and+the+Potential+for+Tick-Borne+Relapsing+Fever+in+Florida&rft.au=Schwan%2C+Tom+G%3BRaffel%2C+Sandra+J%3BSchrumpf%2C+Merry+E%3BPolicastro%2C+Paul+F%3BRawlings%2C+Julie+A%3BLane%2C+Robert+S%3BBreitschwerdt%2C+Edward+B%3BPorcella%2C+Stephen+F&rft.aulast=Schwan&rft.aufirst=Tom&rft.date=2005-08-01&rft.volume=43&rft.issue=8&rft.spage=3851&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - A chemotactic response facilitates mosquito salivary gland infection by malaria sporozoites AN - 17656550; 6518450 AB - Sporozoite invasion of mosquito salivary glands is critical for malaria transmission to vertebrate hosts. After release into the mosquito hemocoel, the means by which malaria sporozoites locate the salivary glands is unknown. We developed a Matrigel-based in vitro system to observe and analyze the motility of GFP-expressing Plasmodium berghei sporozoites in the presence of salivary gland products of Anopheles stephensi mosquitoes using temperature-controlled, low-light-level video microscopy. Sporozoites moved toward unheated salivary gland homogenate (SGH) but not to SGH that had been heated at 56 degree C for 30 min. We also investigated the origin of the attracted population. Attraction to SGH was restricted to hemolymph- and oocyst-derived sporozoites; salivary gland-derived sporozoites were not attracted to SGH. These data imply that sporozoites employ a chemotactic response to high molecular mass proteins or carbohydrate-binding proteins to locate salivary glands. This raises the possibility of utilizing anti-chemotactic factors for the development of mosquito transmission blocking agents. JF - Journal of Experimental Biology AU - Akaki, Mayumi AU - Dvorak, James A AD - Biochemical and Biophysical Parasitology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Bethesda, MD 20892-8132, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3211 EP - 3218 PB - Company of Biologists, 140 Cowley Road Cambridge CB4 0DL UK, [URL:http://www.biologists.com/web/index.html] VL - 208 IS - 16 SN - 0022-0949, 0022-0949 KW - Mosquitoes KW - Salivary gland homogenates KW - Sporozoites KW - ASFA 1: Biological Sciences & Living Resources; ASFA 3: Aquatic Pollution & Environmental Quality; Entomology Abstracts KW - Q5 01524:Public health, medicines, dangerous organisms KW - Q1 01306:Physiology, biochemistry, biophysics KW - Z 05206:Medical & veterinary entomology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17656550?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Experimental+Biology&rft.atitle=A+chemotactic+response+facilitates+mosquito+salivary+gland+infection+by+malaria+sporozoites&rft.au=Akaki%2C+Mayumi%3BDvorak%2C+James+A&rft.aulast=Akaki&rft.aufirst=Mayumi&rft.date=2005-08-01&rft.volume=208&rft.issue=16&rft.spage=3211&rft.isbn=&rft.btitle=&rft.title=Journal+of+Experimental+Biology&rft.issn=00220949&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Metallothionein-I/II Double Knockout Mice Are No More Sensitive to the Carcinogenic Effects of Nickel Subsulfide than Wild-Type Mice AN - 17652463; 6486968 AB - Metallothionein (MT) is a high-affinity metal-binding protein thought to mitigate the toxicity of various metals. MT may limit the toxicity of a metal by direct binding or through action as an antioxidant for metals that generate reactive oxygen species. Nickel compounds have carcinogenic potential in humans and animals, possibly by production of oxidative stress. The impact of MT deficiency on the carcinogenic effects of nickel is unknown. Thus, groups (n = 25) of male MT-I/II double knockout (MT-null) or MT wild-type (WT) mice were exposed to a single treatment of nickel (0.5 or 1.0 mg Ni sub(3)S sub(2)/site, intramuscularly, [i.m.], into both hind legs), or left untreated (control) and observed over the next 104 weeks. There were no differences in the incidence of spontaneous tumors in MT-null and WT mice. Nickel induced injection site fibrosarcomas in a dose-related fashion to a similar extent in both WT and MT-null mice. Nickel-treatment had no effect on total lung tumor incidence, although some phenotypic-specific differences occurred in the proportion of benign and malignant pulmonary tumors. Overall, MT-null mice appear no more sensitive to the carcinogenic effects of nickel than WT mice. Thus, poor MT production does not appear to be a predisposing factor for nickel carcinogenesis. JF - International Journal of Toxicology AU - Waalkes, M P AU - Liu, J AU - Kasprzak, K S AU - Diwan, BA AD - Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at the National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 215 EP - 220 VL - 24 IS - 4 SN - 1091-5818, 1091-5818 KW - Toxicology Abstracts KW - X 24164:Pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17652463?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Toxicology&rft.atitle=Metallothionein-I%2FII+Double+Knockout+Mice+Are+No+More+Sensitive+to+the+Carcinogenic+Effects+of+Nickel+Subsulfide+than+Wild-Type+Mice&rft.au=Waalkes%2C+M+P%3BLiu%2C+J%3BKasprzak%2C+K+S%3BDiwan%2C+BA&rft.aulast=Waalkes&rft.aufirst=M&rft.date=2005-08-01&rft.volume=24&rft.issue=4&rft.spage=215&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Toxicology&rft.issn=10915818&rft_id=info:doi/10.1080%2F10915810591000668 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1080/10915810591000668 ER - TY - JOUR T1 - Recommendations for the Design and Use of Standard Virus Panels To Assess Neutralizing Antibody Responses Elicited by Candidate Human Immunodeficiency Virus Type 1 Vaccines AN - 17649752; 6477060 JF - Journal of Virology AU - Mascola, John R AU - D'Souza, Patricia AU - Gilbert, Peter AU - Hahn, Beatrice H AU - Haigwood, Nancy L AU - Morris, Lynn AU - Petropoulos, Christos J AU - Polonis, Victoria R AU - Sarzotti, Marcella AU - Montefiori, David C AD - Vaccine Research Center. Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 10103 EP - 10107 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 16 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts KW - V 22002:AIDS: Molecular and in vitro aspects KW - W3 33240:Immunology KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17649752?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Recommendations+for+the+Design+and+Use+of+Standard+Virus+Panels+To+Assess+Neutralizing+Antibody+Responses+Elicited+by+Candidate+Human+Immunodeficiency+Virus+Type+1+Vaccines&rft.au=Mascola%2C+John+R%3BD%27Souza%2C+Patricia%3BGilbert%2C+Peter%3BHahn%2C+Beatrice+H%3BHaigwood%2C+Nancy+L%3BMorris%2C+Lynn%3BPetropoulos%2C+Christos+J%3BPolonis%2C+Victoria+R%3BSarzotti%2C+Marcella%3BMontefiori%2C+David+C&rft.aulast=Mascola&rft.aufirst=John&rft.date=2005-08-01&rft.volume=79&rft.issue=16&rft.spage=10103&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Specificity of Legionella pneumophila and Coxiella burnetii Vacuoles and Versatility of Legionella pneumophila Revealed by Coinfection AN - 17642171; 6475985 AB - Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niches, African green monkey kidney epithelial (Vero) cells, A/J mouse bone marrow-derived macrophages, human macrophages, and human dendritic cells (DC) containing mature C. burnetii replication vacuoles were superinfected with L. pneumophila, and then the acidity, lysosome-associated membrane protein (LAMP) content, and cohabitation of mature replication vacuoles was assessed. In all cell types, wild-type L. pneumophila occupied distinct vacuoles in close association with acidic, LAMP-positive C. burnetii replication vacuoles. In murine macrophages, but not primate macrophages, DC, or epithelial cells, L. pneumophila replication vacuoles were acidic and LAMP positive. Unlike wild-type L. pneumophila, type IV secretion-deficient dotA mutants trafficked to lysosome-like C. burnetii vacuoles in Vero cells where they survived but failed to replicate. In primate macrophages, DC, or epithelial cells, growth of L. pneumophila was as robust in superinfected cell cultures as in those singly infected. Thus, despite their noted similarities, L. pneumophila and C. burnetii are exquisitely adapted for replication in unique replication vacuoles, and factors that maintain the C. burnetii replication vacuole do not alter biogenesis of an adjacent L. pneumophila replication vacuole. Moreover, L. pneumophila can replicate efficiently in either lysosomal vacuoles of A/J mouse cells or in nonlysosomal vacuoles of primate cells. JF - Infection and Immunity AU - Sauer, John-Demian AU - Shannon, Jeffrey G AU - Howe, Dale AU - Hayes, Stanley F AU - Swanson, Michele S AU - Heinzen, Robert A AD - Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S. 4th St., Hamilton, Montana 59840. Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4494 EP - 4504 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - N 14025:RNA/DNA role in infection & immune response KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17642171?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Parenting%3A+Science+and+Practice&rft.atitle=The+Bio-Culture+of+Parenting%3A+Evidence+From+Five+Cultural+Communities&rft.au=Keller%2C+Heidi%3BLohaus%2C+Arnold%3BKuensemueller%2C+Petra%3BAbels%2C+Monika%3BYovsi%2C+Relindis%3BVoelker%2C+Susanne%3BJensen%2C+Henning%3BPapaligoura%2C+Zaira%3BRosabal-Coto%2C+Mariano%3BKulks%2C+Daniela%3BMohita%2C+Preana&rft.aulast=Keller&rft.aufirst=Heidi&rft.date=2004-01-01&rft.volume=4&rft.issue=1&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Parenting%3A+Science+and+Practice&rft.issn=15295192&rft_id=info:doi/10.1207%2Fs15327922par0401_2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Degradation of Chlamydia pneumoniae by Peripheral Blood Monocytic Cells AN - 17635290; 6475992 AB - Chlamydia pneumoniae is a common human respiratory pathogen that has been associated with a variety of chronic diseases, including atherosclerosis. The role of this organism in the pathogenesis of atherosclerosis remains unknown. A key question is how C. pneumoniae is transferred from the site of primary infection to a developing atherosclerotic plaque. It has been suggested that circulating monocytes could be vehicles for dissemination of C. pneumoniae since the organism has been detected in peripheral blood monocytic cells (PBMCs). In this study we focused on survival of C. pneumoniae within PBMCs isolated from the blood of healthy human donors. We found that C. pneumoniae does not grow and multiply in cultured primary monocytes. In C. pneumoniae-infected monocyte-derived macrophages, growth of the organism was very limited, and the majority of the bacteria were eradicated. We also found that the destruction of C. pneumoniae within infected macrophages resulted in a gradual diminution of chlamydial antigens, although some of these antigens could be detected for days after the initial infection. The detected antigens present in infected monocytes and monocyte-derived macrophages represented neither chlamydial inclusions nor intact organisms. The use of {N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]}-6-aminocaproyl-D-erythro- s phingosine as a vital stain for chlamydiae proved to be a sensitive method for identifying rare C. pneumoniae inclusions and was useful in the detection of even aberrant developmental forms. JF - Infection and Immunity AU - Wolf, Katerina AU - Fischer, Elizabeth AU - Hackstadt, Ted AD - Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana 59840 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4560 EP - 4570 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17635290?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Degradation+of+Chlamydia+pneumoniae+by+Peripheral+Blood+Monocytic+Cells&rft.au=Wolf%2C+Katerina%3BFischer%2C+Elizabeth%3BHackstadt%2C+Ted&rft.aulast=Wolf&rft.aufirst=Katerina&rft.date=2005-08-01&rft.volume=73&rft.issue=8&rft.spage=4560&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - High-Throughput Screening of 11 beta -Hydroxysteroid Dehydrogenase Type 1 in Scintillation Proximity Assay Format AN - 17431044; 6538052 AB - 11 beta -Hydroxysteroid dehydrogenase type-1 (11 beta -HSD1) is a potential target for the treatment of diabetes, obesity, and hyperlipidemia. This enzyme is mainly responsible for reactivating glucocorticoid hormone inside cells such as adipose cells and liver cells by converting the inactive cortisone to active cortisol. Enzyme assays for 11 beta -HSD1 involve either a thin-layer chromatography or high-performance liquid chromatography step to separate cortisol from the substrate cortisone. This additional step is labor intensive and increases the assay time, which limits assay throughput. A homogenous scintillation proximity assay-based method has been recently developed that enables high-throughput screening of 11 beta -HSD1 inhibitors. We have applied this novel 11 beta -HSD1 assay to screening a large-size compound collection and identified several structural classes of lead compounds that selectively inhibit the activity of 11 beta -HSD1. JF - Assay and Drug Development Technologies AU - Solly, K AU - Mundt, S S AU - Zokian, HJ AU - Ding, GJ-F AU - Hermanowski-Vosatka, A AU - Strulovici, B AU - Zheng, W AD - National Institutes of Health, National Human Genome Research Institute, NIH Chemical Genomics Center, 9800 Medical Center Drive, MSC: 3370, Bethesda, MD 20892, USA, wzheng@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 377 VL - 3 IS - 4 SN - 1540-658X, 1540-658X KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - High-performance liquid chromatography KW - Obesity KW - Hydrocortisone KW - 11^b-Hydroxysteroid dehydrogenase KW - Hepatocytes KW - Hyperlipidemia KW - Hormones KW - Glucocorticoids KW - Diabetes mellitus KW - Scintillation KW - high-throughput screening KW - Thin-layer chromatography KW - W 30965:Miscellaneous, Reviews KW - W3 33250:Methods: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17431044?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Assay+and+Drug+Development+Technologies&rft.atitle=High-Throughput+Screening+of+11+beta+-Hydroxysteroid+Dehydrogenase+Type+1+in+Scintillation+Proximity+Assay+Format&rft.au=Solly%2C+K%3BMundt%2C+S+S%3BZokian%2C+HJ%3BDing%2C+GJ-F%3BHermanowski-Vosatka%2C+A%3BStrulovici%2C+B%3BZheng%2C+W&rft.aulast=Solly&rft.aufirst=K&rft.date=2005-08-01&rft.volume=3&rft.issue=4&rft.spage=377&rft.isbn=&rft.btitle=&rft.title=Assay+and+Drug+Development+Technologies&rft.issn=1540658X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - 11^b-Hydroxysteroid dehydrogenase; Hydrocortisone; high-throughput screening; Scintillation; High-performance liquid chromatography; Thin-layer chromatography; Glucocorticoids; Obesity; Hormones; Hepatocytes; Diabetes mellitus; Hyperlipidemia ER - TY - JOUR T1 - Dietary Benzo[a]Pyrene Intake and Risk of Colorectal Adenoma AN - 17406020; 6522172 AB - We carried out a clinic-based case-control study specifically designed to address the hypothesis that dietary intake of polycyclic aromatic hydrocarbons (PAH) is associated with colorectal adenoma risk. We developed a food frequency questionnaire with detailed questions on meat-cooking methods and doneness levels and a benzo[a]pyrene (BaP) database (as a surrogate for total carcinogenic PAHs) based on the collection and analysis of a wide range of food samples. We estimated BaP intake derived from meat and from all foods to test its relationship with risk of colorectal adenomas. The median (10th and 90th percentiles) BaP intake in controls was 5 ng/d (0.2 and 66 ng/d) estimated from meat and 73 ng/d (35 and 140 ng/d) from all foods. In cases, median BaP intake was 17 ng/d (0.5 and 101 ng/d) from meat and 76 ng/d (44 and 163 ng/d) from all foods. Multivariate analysis was carried out on 146 cases and 228 controls. The odds ratios (95% confidence interval) for dietary BaP from meat with the first quintile as the reference group were 1.19 (0.51-2.80) for the second quintile, 1.71 (0.76-3.83) for the third quintile, 2.16 (0.96-4.86) for the fourth quintile, and 2.82 (1.24-6.43) for the fifth quintile (P sub(trend) = 0.01). Increased risk of colorectal adenomas was more strongly associated with BaP intake estimated from all foods: 2.61 (1.08-6.29) for the second quintile, 4.21 (1.79-9.91) for the third quintile, 2.45 (0.98-6.12) for the fourth quintile, and 5.60 (2.20-14.20) for the fifth quintile (P sub(trend) = 0.002). This study provides evidence that dietary BaP plays a role in colorectal adenoma etiology. JF - Cancer Epidemiology, Biomarkers & Prevention AU - Sinha, Rashmi AU - Kulldorff, Martin AU - Gunter, Marc J AU - Strickland, Paul AU - Rothman, Nathaniel AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Rockville, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2030 EP - 2034 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 8 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts KW - Meat KW - Databases KW - Polycyclic aromatic hydrocarbons KW - Multivariate analysis KW - Food KW - Benzo(a)pyrene KW - Adenoma KW - Dietary intake KW - biomarkers KW - X 24190:Polycyclic hydrocarbons UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17406020?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Dietary+Benzo%5Ba%5DPyrene+Intake+and+Risk+of+Colorectal+Adenoma&rft.au=Sinha%2C+Rashmi%3BKulldorff%2C+Martin%3BGunter%2C+Marc+J%3BStrickland%2C+Paul%3BRothman%2C+Nathaniel&rft.aulast=Sinha&rft.aufirst=Rashmi&rft.date=2005-08-01&rft.volume=14&rft.issue=8&rft.spage=2030&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Meat; Databases; Polycyclic aromatic hydrocarbons; Multivariate analysis; Food; Benzo(a)pyrene; biomarkers; Dietary intake; Adenoma ER - TY - JOUR T1 - The agr Radiation: an Early Event in the Evolution of Staphylococci AN - 17402946; 6517740 AB - agr is a global regulatory system in the staphylococci, operating by a classical two-component signaling module and controlling the expression of most of the genes encoding extracellular virulence factors. As it is autoinduced by a peptide, encoded within the locus, that is the ligand for the signal receptor, it is a sensor of population density or a quorum sensor and is the only known quorum-sensing system in the genus. agr is conserved throughout the staphylococci but has diverged along lines that appear to parallel speciation and subspeciation within the genus. This divergence has given rise to a novel type of interstrain and interspecies cross-inhibition that represents a fundamental aspect of the organism's biology and may be a predominant feature of the evolutionary forces that have driven it. We present evidence, using a newly developed, luciferase-based agr typing scheme, that the evolutionary divergence of the agr system was an early event in the evolution of the staphylococci and long preceded the development of the nucleotide polymorphisms presently used for genotyping. These polymorphisms developed, for the most part, within different agr groups; mobile genetic elements appear also to have diffused recently and, with a few notable exceptions, have come to reside largely indiscriminately within the several agr groups. JF - Journal of Bacteriology AU - Wright, Jesse SIII AU - Traber, Katrina E AU - Corrigan, Rebecca AU - Benson, Sarah A AU - Musser, James M AU - Novick, Richard P AD - Molecular Pathogenesis Program and Department of Microbiology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, New York 10016. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840. Center for Human Bacterial Pathogenesis Research, Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5585 EP - 5594 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Speciation KW - virulence factors KW - Gene polymorphism KW - Genotyping KW - Staphylococcus KW - Population density KW - Evolution KW - Nucleotides KW - Signal transduction KW - G 07320:Bacterial genetics KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17402946?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=The+agr+Radiation%3A+an+Early+Event+in+the+Evolution+of+Staphylococci&rft.au=Wright%2C+Jesse+SIII%3BTraber%2C+Katrina+E%3BCorrigan%2C+Rebecca%3BBenson%2C+Sarah+A%3BMusser%2C+James+M%3BNovick%2C+Richard+P&rft.aulast=Wright&rft.aufirst=Jesse&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5585&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Speciation; virulence factors; Genotyping; Gene polymorphism; Population density; Nucleotides; Evolution; Signal transduction; Staphylococcus ER - TY - JOUR T1 - Cellular Mechanisms for Low-Dose Ionizing Radiation-Induced Perturbation of the Breast Tissue Microenvironment AN - 17399440; 6517116 AB - Radiation exposure is an important form of environmental carcinogen and has been associated with increased risk of breast cancer. Epigenetic events, especially those involving alterations in the breast stromal microenvironment, may play an important role in radiation-induced carcinogenesis but remain not well understood. We here show that human mammary stromal fibroblasts respond to protracted low-dose ionizing radiation exposures by displaying a senescence-like phenotype. Using a three-dimensional coculture system to model the interactions of different mammary cell types with their neighbors and with their environment, we provide a direct experimental proof that ionizing radiation-induced senescence-like fibroblasts significantly perturb the mammary stromal microenvironment, which is highlighted by impaired formation of pseudopodia networks due to marked cytoskeletal alterations in senescence-like fibroblasts and increased extracellular matrix degradation because of the up-regulation of multiple secreted matrix metalloproteinases. Within such a perturbed environment, mammary ductal morphogenesis is completely disrupted and epithelial cells instead grow into enlarged cystic structures, which further develop and become disorganized cell masses on inactivation of cellular death pathways. Breast carcinoma cells growing in such an environment are enabled to fully express their malignant potential as evidenced by the alpha 6 beta 4 integrin/phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin pathway-dependent invasive growth. Our results suggest that ionizing radiation, in addition to causing gene mutations in epithelial cells, can contribute to breast carcinogenesis by perturbing the tissue microenvironment that leads to dysregulated cell-cell and cell-matrix interactions. JF - Cancer Research AU - Tsai, Kelvin KC AU - Chuang, Eric Yao-Yu AU - Little, John B AU - Yuan, Zhi-Min AD - Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts and Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 6734 EP - 6744 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 15 SN - 0008-5472, 0008-5472 KW - Toxicology Abstracts KW - Epithelial cells KW - X radiation KW - Matrix metalloproteinase KW - Carcinogens KW - Fibroblasts KW - Cytoskeleton KW - 1-Phosphatidylinositol 3-kinase KW - Ionizing radiation KW - Carcinogenesis KW - AKT protein KW - Microenvironments KW - Breast carcinoma KW - X 24210:Radiation & radioactive materials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17399440?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=Cellular+Mechanisms+for+Low-Dose+Ionizing+Radiation-Induced+Perturbation+of+the+Breast+Tissue+Microenvironment&rft.au=Tsai%2C+Kelvin+KC%3BChuang%2C+Eric+Yao-Yu%3BLittle%2C+John+B%3BYuan%2C+Zhi-Min&rft.aulast=Tsai&rft.aufirst=Kelvin&rft.date=2005-08-01&rft.volume=65&rft.issue=15&rft.spage=6734&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Cytoskeleton; Epithelial cells; 1-Phosphatidylinositol 3-kinase; X radiation; Ionizing radiation; Carcinogenesis; AKT protein; Matrix metalloproteinase; Microenvironments; Breast carcinoma; Carcinogens; Fibroblasts ER - TY - JOUR T1 - The Bacteriophage P1 hot Gene Product Can Substitute for the Escherichia coli DNA Polymerase III theta Subunit AN - 17397837; 6517734 AB - The theta subunit (holE gene product) of Escherichia coli DNA polymerase (Pol) III holoenzyme is a tightly bound component of the polymerase core. Within the core ( alpha - epsilon - theta ), the alpha and epsilon subunits carry the DNA polymerase and 3' proofreading functions, respectively, while the precise function of theta is unclear. holE homologs are present in genomes of other enterobacteriae, suggestive of a conserved function. Putative homologs have also been found in the genomes of bacteriophage P1 and of certain conjugative plasmids. The presence of these homologs is of interest, because these genomes are fully dependent on the host replication machinery and contribute few, if any, replication factors themselves. To study the role of these theta homologs, we have constructed an E. coli strain in which holE is replaced by the P1 homolog, hot. We show that hot is capable of substituting for holE when it is assayed for its antimutagenic action on the proofreading-impaired dnaQ49 mutator, which carries a temperature-sensitive epsilon subunit. The ability of hot to substitute for holE was also observed with other, although not all, dnaQ mutator alleles tested. The data suggest that the P1 hot gene product can substitute for the theta subunit and is likely incorporated in the Pol III complex. We also show that overexpression of either theta or Hot further suppresses the dnaQ49 mutator phenotype. This suggests that the complexing of dnaQ49- epsilon with theta is rate limiting for its ability to proofread DNA replication errors. The possible role of hot for bacteriophage P1 is discussed. JF - Journal of Bacteriology AU - Chikova, Anna K AU - Schaaper, Roel M AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Science, Moscow, 123098, Russia Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5528 EP - 5536 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts; Genetics Abstracts KW - Genomes KW - Phages KW - DNA biosynthesis KW - Data processing KW - Replication KW - DNA-directed DNA polymerase KW - Escherichia coli KW - Plasmids KW - Proofreading KW - J 02725:DNA KW - G 07320:Bacterial genetics KW - N 14820:DNA Metabolism & Structure KW - V 22070:Phage-host interactions including lysogeny & transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17397837?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=The+Bacteriophage+P1+hot+Gene+Product+Can+Substitute+for+the+Escherichia+coli+DNA+Polymerase+III+theta+Subunit&rft.au=Chikova%2C+Anna+K%3BSchaaper%2C+Roel+M&rft.aulast=Chikova&rft.aufirst=Anna&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5528&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Phages; Genomes; DNA biosynthesis; Data processing; Replication; DNA-directed DNA polymerase; Plasmids; Proofreading; Escherichia coli ER - TY - JOUR T1 - Biological dust exposure in the workplace is a risk factor for chronic obstructive pulmonary disease AN - 17397768; 6519789 AB - BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity and mortality. Although the main risk factor is smoking, 15-19% of COPD even in smokers has been attributed to occupational exposures. The aim of this study was to investigate the association between occupational exposure and risk of COPD. METHODS: Participants were part of a cross sectional study of risk factors for COPD. A total of 1232 completed a detailed respiratory questionnaire, spirometric testsing and measurement of gas transfer. Job histories were coded according to the International Standard Classification of Occupations. These codes were then used to establish occupational exposures using the ALOHA job exposure matrix. RESULTS: The prevalence of emphysema was 2.4%, chronic obstructive bronchitis 1.8%, and COPD 3.4%. Subjects ever exposed to biological dusts had an increased risk of chronic obstructive bronchitis (OR 3.19; 95% CI 1.27 to 7.97), emphysema (OR 3.18; 95% CI 1.41 to 7.13), and COPD (OR 2.70, 95% CI 1.39 to 5.23). These risks were higher in women than in men. For biological dust, the risk of emphysema and COPD was also significantly increased in both the duration of exposure categories, again in women but not in men. No significant increased risks for COPD were found for mineral dust (OR 1.13; 95% CI 0.57 to 2.27) or gases/fumes (OR 1.63; 95% CI 0.83 to 3.22). CONCLUSION: In this general population sample of adults, occupational exposures to biological dusts were associated with an increased risk of COPD which was higher in women. Preventive strategies should be aimed at reducing exposure to these agents in the workplace. JF - Thorax AU - Matheson, M C AU - Benke, G AU - Raven, J AU - Sim, M R AU - Kromhout, H AU - Vermeulen, R AU - Johns, D P AU - Walters, E H AU - Abramson, M J AD - Department of Epidemiology and Preventive Medicine, Monash University, Victoria, Australia. Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital, Victoria, Australia. Division of Occupational and Environmental Health, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands. Occupational and Environmental Epidemiology Branch, National Cancer Institute, Rockville, MD 20852-7240, USA. Cardio-Respiratory Research Group, School of Medicine, University of Tasmania, Hobart, Australia Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 645 EP - 651 PB - B M J Publishing Group, B.M.A. House Tavistock Sq. London WC1H 9JR UK VL - 60 IS - 8 SN - 0040-6376, 0040-6376 KW - Health & Safety Science Abstracts; Toxicology Abstracts; Immunology Abstracts KW - Historical account KW - Morbidity KW - Dust KW - Smoking KW - Classification KW - Risk factors KW - Bronchitis KW - Occupational exposure KW - chronic obstructive pulmonary disease KW - International standardization KW - Mortality KW - Emphysema KW - Fumes KW - Obstructive lung disease KW - International standards KW - Gases KW - classification KW - Minerals KW - X 24240:Miscellaneous KW - F 06364:Respiratory System: Animal KW - H 1000:Occupational Safety and Health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17397768?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Thorax&rft.atitle=Biological+dust+exposure+in+the+workplace+is+a+risk+factor+for+chronic+obstructive+pulmonary+disease&rft.au=Matheson%2C+M+C%3BBenke%2C+G%3BRaven%2C+J%3BSim%2C+M+R%3BKromhout%2C+H%3BVermeulen%2C+R%3BJohns%2C+D+P%3BWalters%2C+E+H%3BAbramson%2C+M+J&rft.aulast=Matheson&rft.aufirst=M&rft.date=2005-08-01&rft.volume=60&rft.issue=8&rft.spage=645&rft.isbn=&rft.btitle=&rft.title=Thorax&rft.issn=00406376&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Emphysema; Mortality; Fumes; Obstructive lung disease; Dust; Morbidity; International standards; Smoking; Gases; Classification; Risk factors; Bronchitis; Minerals; Occupational exposure; Historical account; classification; International standardization; chronic obstructive pulmonary disease ER - TY - JOUR T1 - Prevention and Treatment of Cutaneous Leishmaniasis in Primates by Using Synthetic Type D/A Oligodeoxynucleotides Expressing CpG Motifs AN - 17384845; 6476034 AB - Oligodeoxynucleotides (ODN) containing CpG motifs mimic microbial DNA and are recognized by toll-like receptor 9 on immune cells. The resulting response limits the early spread of infectious organisms and promotes the development of adaptive immunity. In this regard, CpG ODN show promise as immunoprotective agents and as vaccine adjuvants. Previous studies of nonhuman primates showed that administration of CpG ODN type D (also known as type A) at the site of infection 3 days before and after a challenge with Leishmania major enhanced host resistance and reduced the lesion's severity. In this study, we show that systemic administration of D/A ODN limits the size of lesions following an intradermal infection with L. major. Importantly, the reduced morbidity was not associated with a reduction in long-term immunity, as such treated macaques were still protected following a secondary challenge. Finally, administration of D/A ODN to macaques that had established cutaneous lesions reduced the severity of the lesions, suggesting a potential role for CpG ODN in L. major treatment. Together, these findings support the development of clinical studies to assess the use of CpG ODN types D/A as immunoprotective and therapeutic agents. JF - Infection and Immunity AU - Flynn, Barbara AU - Wang, Vivian AU - Sacks, David L AU - Seder, Robert A AU - Verthelyi, Daniela AD - Division of Therapeutic Proteins, Center for Drug Evaluation and Review, Food and Drug Administration, Washington, D.C. Vaccine Research Center. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4948 EP - 4954 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Primates KW - Macaques KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology; Biochemistry Abstracts 2: Nucleic Acids KW - Adjuvants KW - Infection KW - Oligonucleotides KW - Morbidity KW - Leishmania major KW - Macaca KW - Immunity KW - CpG islands KW - Vaccines KW - Toll-like receptors KW - Cutaneous leishmaniasis KW - K 03086:Immunology & vaccination KW - N 14025:RNA/DNA role in infection & immune response KW - F 06100:Vaccines - active immunity KW - W3 33345:DNA vaccines KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17384845?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Prevention+and+Treatment+of+Cutaneous+Leishmaniasis+in+Primates+by+Using+Synthetic+Type+D%2FA+Oligodeoxynucleotides+Expressing+CpG+Motifs&rft.au=Flynn%2C+Barbara%3BWang%2C+Vivian%3BSacks%2C+David+L%3BSeder%2C+Robert+A%3BVerthelyi%2C+Daniela&rft.aulast=Flynn&rft.aufirst=Barbara&rft.date=2005-08-01&rft.volume=73&rft.issue=8&rft.spage=4948&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Primates; Macaca; Leishmania major; CpG islands; Oligonucleotides; Immunity; Infection; Morbidity; Vaccines; Adjuvants; Toll-like receptors; Cutaneous leishmaniasis ER - TY - JOUR T1 - Replicating Rather than Nonreplicating Adenovirus-Human Immunodeficiency Virus Recombinant Vaccines Are Better at Eliciting Potent Cellular Immunity and Priming High-Titer Antibodies AN - 17378478; 6477070 AB - A major challenge in combating the human immunodeficiency virus (HIV) epidemic is the development of vaccines capable of inducing potent, persistent cellular immunity and broadly reactive neutralizing antibody responses to HIV type 1 (HIV-1). We report here the results of a preclinical trial using the chimpanzee model to investigate a combination vaccine strategy involving sequential priming immunizations with different serotypes of adenovirus (Ad)/HIV-1 sub(MN)env/rev recombinants and boosting with an HIV envelope subunit protein, oligomeric HIV sub(SF162) gp140 Delta V2. The immunogenicities of replicating and nonreplicating Ad/HIV-1 sub(MN)env/rev recombinants were compared. Replicating Ad/HIV recombinants were better at eliciting HIV-specific cellular immune responses and better at priming humoral immunity against HIV than nonreplicating Ad-HIV recombinants carrying the same gene insert. Enhanced cellular immunity was manifested by a greater frequency of HIV envelope-specific gamma interferon-secreting peripheral blood lymphocytes and better priming of T-cell proliferative responses. Enhanced humoral immunity was seen in higher anti-envelope binding and neutralizing antibody titers and better induction of antibody-dependent cellular cytotoxicity. More animals primed with replicating Ad recombinants mounted neutralizing antibodies against heterologous R5 viruses after one or two booster immunizations with the mismatched oligomeric HIV-1 sub(SF162) gp140 Delta V2 protein. These results support continued development of the replicating Ad-HIV recombinant vaccine approach and suggest that the use of replicating vectors for other vaccines may prove fruitful. JF - Journal of Virology AU - Peng, Bo AU - Wang, Liqun Rejean AU - Gomez-Roman, Victor Raul AU - Davis-Warren, Alberta AU - Montefiori, David C AU - Kalyanaraman, V S AU - Venzon, David AU - Zhao, Jun AU - Kan, Elaine AU - Rowell, Thomas J AU - Murthy, Krishna K AU - Srivastava, Indresh AU - Barnett, Susan W AU - Robert-Guroff, Marjorie AD - Vaccine Branch. Biostatistics and Data Management Section, National Cancer Institute, Bethesda, Maryland 20892. Department of Surgery, Laboratory for AIDS Vaccine Research & Development, Duke University Medical Center, Durham, North Carolina 27710. Advanced BioScience Laboratories, Inc., Kensington, Maryland 20895. Chiron Corp., Emeryville, California 94608-2916. University of Louisiana at Lafayette, New Iberia Research Center, New Iberia, Louisiana 70560. Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 10200 EP - 10209 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 16 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Genetics Abstracts; Immunology Abstracts; Virology & AIDS Abstracts KW - gamma -Interferon KW - Serotypes KW - Epidemics KW - Adenovirus KW - Peripheral blood KW - Lymphocytes KW - Clinical trials KW - Immunization KW - Immunity (humoral) KW - Antibodies KW - Cytotoxicity KW - Envelopes KW - Immunity (cell-mediated) KW - Immunogenicity KW - Human immunodeficiency virus 1 KW - Envelope protein KW - Lymphocytes T KW - Vaccines KW - W3 33365:Vaccines (other) KW - G 07240:Immunogenetics KW - G 07313:Viruses KW - F 06100:Vaccines - active immunity KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17378478?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Replicating+Rather+than+Nonreplicating+Adenovirus-Human+Immunodeficiency+Virus+Recombinant+Vaccines+Are+Better+at+Eliciting+Potent+Cellular+Immunity+and+Priming+High-Titer+Antibodies&rft.au=Peng%2C+Bo%3BWang%2C+Liqun+Rejean%3BGomez-Roman%2C+Victor+Raul%3BDavis-Warren%2C+Alberta%3BMontefiori%2C+David+C%3BKalyanaraman%2C+V+S%3BVenzon%2C+David%3BZhao%2C+Jun%3BKan%2C+Elaine%3BRowell%2C+Thomas+J%3BMurthy%2C+Krishna+K%3BSrivastava%2C+Indresh%3BBarnett%2C+Susan+W%3BRobert-Guroff%2C+Marjorie&rft.aulast=Peng&rft.aufirst=Bo&rft.date=2005-08-01&rft.volume=79&rft.issue=16&rft.spage=10200&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - gamma -Interferon; Epidemics; Serotypes; Peripheral blood; Lymphocytes; Clinical trials; Immunization; Immunity (humoral); Cytotoxicity; Antibodies; Envelopes; Immunity (cell-mediated); Immunogenicity; Envelope protein; Lymphocytes T; Vaccines; Human immunodeficiency virus 1; Adenovirus ER - TY - JOUR T1 - PAMAM Dendrimer Based Macromolecules as Improved Contrast Agents AN - 17360432; 6436873 AB - Dendrimers are an attractive platform for macromolecular imaging due to the presence of multiple terminal groups on the exterior of the molecule. Through application of appropriate metal ion chelates, large numbers of metal ions for imaging (paramagnetic or radioopaque) and therapy (radioactive particle emitters) may be conjugated to the dendrimer in combination with a targeting vector, through classic organic synthetic techniques. Thus, a large amount of these metal ions potentially may be site specifically delivered directly into the body with the dendrimer as the vehicle with the targeting vector directing the modified dendrimer. The development of targeted macromolecular agents with acceptable blood retention times and selective organ uptake then has the potential for various biological applications. A review of comparative studies of dendrimers with various externally appended imaging and targeting agents is presented herein. JF - Molecular Pharmaceutics AU - Venditto, V J AU - Regino, CAS AU - Brechbiel, M W AD - Radioimmune & Inorganic Chemistry Section, Radiation Oncology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 302 EP - 311 VL - 2 IS - 4 SN - 1543-8384, 1543-8384 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Metals KW - Ions KW - Blood KW - Macromolecules KW - Reviews KW - Contrast media KW - Chelates KW - imaging KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17360432?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Pharmaceutics&rft.atitle=PAMAM+Dendrimer+Based+Macromolecules+as+Improved+Contrast+Agents&rft.au=Venditto%2C+V+J%3BRegino%2C+CAS%3BBrechbiel%2C+M+W&rft.aulast=Venditto&rft.aufirst=V&rft.date=2005-08-01&rft.volume=2&rft.issue=4&rft.spage=302&rft.isbn=&rft.btitle=&rft.title=Molecular+Pharmaceutics&rft.issn=15438384&rft_id=info:doi/10.1021%2Fmp050019e LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Blood; Ions; Metals; Macromolecules; Reviews; Contrast media; Chelates; imaging DO - http://dx.doi.org/10.1021/mp050019e ER - TY - JOUR T1 - Lyme disease agent borrows a practical coat AN - 17353221; 6422760 AB - The bacterium that causes Lyme disease is a manipulative creature. This pathogen exploits a component in the saliva of its vector, a tick, to facilitate invasion of vertebrate hosts. JF - Nature Medicine AU - Rosa, P AD - Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA, prosa@niaid.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 831 EP - 832 VL - 11 IS - 8 SN - 1078-8956, 1078-8956 KW - Microbiology Abstracts B: Bacteriology KW - J 02855:Human Bacteriology: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17353221?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Medicine&rft.atitle=Lyme+disease+agent+borrows+a+practical+coat&rft.au=Rosa%2C+P&rft.aulast=Rosa&rft.aufirst=P&rft.date=2005-08-01&rft.volume=11&rft.issue=8&rft.spage=831&rft.isbn=&rft.btitle=&rft.title=Nature+Medicine&rft.issn=10788956&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Smaller head of the hippocampus in Gulf War-related posttraumatic stress disorder. AN - 68001586; 15967648 AB - Reductions in hippocampal volume and impairment in short-term verbal memory have been reported in Vietnam combat veterans with posttraumatic stress disorder (PTSD) and in women with abuse-related PTSD. The present investigation evaluated hippocampal volume and memory in Gulf War veterans. This research is timely given the ongoing war in Iraq and the anticipated high rates of PTSD among returning combat soldiers. Fourteen veterans with PTSD related to traumatic experiences during the Gulf War (1990-1991), 23 deployed veterans without PTSD, 22 non-deployed reservists and 29 healthy civilians were studied. Volumes of the hippocampus, temporal lobe, and whole brain were measured on coronal MRI scans, and hippocampal mediated memory function was evaluated. The head of the hippocampus was the only subregion that was significantly smaller in Gulf War veterans with PTSD than in healthy civilians. Deployed veterans with PTSD, deployed veterans without PTSD, and non-deployed reservists had significantly smaller whole hippocampal volume and lower scores on immediate and delayed verbal and visual retrieval compared with healthy civilians. JF - Psychiatry research AU - Vythilingam, Meena AU - Luckenbaugh, David A AU - Lam, Thomas AU - Morgan, Charles A AU - Lipschitz, Deborah AU - Charney, Dennis S AU - Bremner, J Douglas AU - Southwick, Steven M AD - Mood and Anxiety Disorders Program, National Institute of Mental Health, MAP, 15K North Drive, Room #111, MSC 2670, Bethesda, MD 20892-2670, USA. meena.vythi@nih.gov Y1 - 2005/07/30/ PY - 2005 DA - 2005 Jul 30 SP - 89 EP - 99 VL - 139 IS - 2 SN - 0165-1781, 0165-1781 KW - Index Medicus KW - Temporal Lobe -- anatomy & histology KW - Magnetic Resonance Imaging KW - Cognition Disorders -- diagnosis KW - Cognition Disorders -- epidemiology KW - Humans KW - Adult KW - Military Personnel -- statistics & numerical data KW - Observer Variation KW - Neuropsychological Tests KW - Male KW - Functional Laterality -- physiology KW - Female KW - Temporal Lobe -- physiopathology KW - Stress Disorders, Post-Traumatic -- etiology KW - Hippocampus -- physiopathology KW - Stress Disorders, Post-Traumatic -- physiopathology KW - Persian Gulf Syndrome -- psychology KW - Hippocampus -- anatomy & histology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68001586?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychiatry+research&rft.atitle=Smaller+head+of+the+hippocampus+in+Gulf+War-related+posttraumatic+stress+disorder.&rft.au=Vythilingam%2C+Meena%3BLuckenbaugh%2C+David+A%3BLam%2C+Thomas%3BMorgan%2C+Charles+A%3BLipschitz%2C+Deborah%3BCharney%2C+Dennis+S%3BBremner%2C+J+Douglas%3BSouthwick%2C+Steven+M&rft.aulast=Vythilingam&rft.aufirst=Meena&rft.date=2005-07-30&rft.volume=139&rft.issue=2&rft.spage=89&rft.isbn=&rft.btitle=&rft.title=Psychiatry+research&rft.issn=01651781&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dose escalation trial designs based on a molecularly targeted endpoint. AN - 67988941; 15909289 AB - Traditional phase I dose-finding studies for chemotoxic agents base dose escalation on toxicity, with escalation continuing until unacceptable toxicity is observed. Recent development of molecularly targeted agents that have little or no toxicity in the therapeutic dose range has raised questions over the best study designs for phase I studies. Two types of designs are proposed and evaluated in this paper. In these designs, escalation is based on a binary response that indicates whether or not the agent has had the desired effect on the molecular target. One design is developed to ensure that if the true target response rate is low there will be a high probability of escalating and if the true target response rate is high there will be a low probability of escalating. The other design is developed to continue to escalate as long as the true response rate is increasing and to stop escalating when the response rate plateaus or decreases. A limited simulation study is performed and the designs are compared with respect to the dose level at the end of escalation and the number of patients treated on study. JF - Statistics in medicine AU - Hunsberger, Sally AU - Rubinstein, Lawrence V AU - Dancey, Janet AU - Korn, Edward L AD - Biometrics Research Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. sallyh@ctep.nci.nih.gov Y1 - 2005/07/30/ PY - 2005 DA - 2005 Jul 30 SP - 2171 EP - 2181 VL - 24 IS - 14 SN - 0277-6715, 0277-6715 KW - Index Medicus KW - Computer Simulation KW - Dose-Response Relationship, Drug KW - Humans KW - Maximum Tolerated Dose KW - Research Design KW - Clinical Trials, Phase I as Topic -- methods KW - Drug Evaluation -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67988941?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Statistics+in+medicine&rft.atitle=Dose+escalation+trial+designs+based+on+a+molecularly+targeted+endpoint.&rft.au=Hunsberger%2C+Sally%3BRubinstein%2C+Lawrence+V%3BDancey%2C+Janet%3BKorn%2C+Edward+L&rft.aulast=Hunsberger&rft.aufirst=Sally&rft.date=2005-07-30&rft.volume=24&rft.issue=14&rft.spage=2171&rft.isbn=&rft.btitle=&rft.title=Statistics+in+medicine&rft.issn=02776715&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-20 N1 - Date created - 2005-06-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena. AN - 68070603; 15897201 AB - Nuclease A (NucA) is a nonspecific endonuclease from Anabaena sp. capable of degrading single- and double-stranded DNA and RNA in the presence of divalent metal ions. We have determined the structure of the delta(2-24),D121A mutant of NucA in the presence of Zn2+ and Mn2+ (PDB code 1ZM8). The mutations were introduced to remove the N-terminal signal peptide and to reduce the activity of the nonspecific nuclease, thereby reducing its toxicity to the Escherichia coli expression system. NucA contains a betabeta alpha metal finger motif and a hydrated Mn2+ ion at the active site. Unexpectedly, NucA was found to contain additional metal binding sites approximately 26 A apart from the catalytic metal binding site. A structural comparison between NucA and the closest analog for which structural data exist, the Serratia nuclease, indicates several interesting differences. First, NucA is a monomer rather than a dimer. Second, there is an unexpected structural homology between the N-terminal segments despite a poorly conserved sequence, which in Serratia includes a cysteine bridge thought to play a regulatory role. In addition, although a sequence alignment had suggested that NucA lacks a proposed catalytic residue corresponding to Arg57 in Serratia, the structure determined here indicates that Arg93 in NucA is positioned to fulfill this role. Based on comparison with DNA-bound nuclease structures of the betabeta alpha metal finger nuclease family and available mutational data on NucA, we propose that His124 acts as a catalytic base, and Arg93 participates in the catalysis possibly through stabilization of the transition state. JF - The Journal of biological chemistry AU - Ghosh, Mahua AU - Meiss, Gregor AU - Pingoud, Alfred AU - London, Robert E AU - Pedersen, Lars C AD - Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/07/29/ PY - 2005 DA - 2005 Jul 29 SP - 27990 EP - 27997 VL - 280 IS - 30 SN - 0021-9258, 0021-9258 KW - Cations KW - 0 KW - Disulfides KW - Ions KW - Protein Sorting Signals KW - Recombinant Proteins KW - Manganese KW - 42Z2K6ZL8P KW - Histidine KW - 4QD397987E KW - RNA KW - 63231-63-0 KW - DNA KW - 9007-49-2 KW - Arginine KW - 94ZLA3W45F KW - Endonucleases KW - EC 3.1.- KW - sugar-nonspecific nuclease KW - Zinc KW - J41CSQ7QDS KW - Cysteine KW - K848JZ4886 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Catalytic Domain KW - Zinc -- chemistry KW - Mutagenesis, Site-Directed KW - Cysteine -- chemistry KW - Amino Acid Motifs KW - Molecular Sequence Data KW - DNA -- chemistry KW - Recombinant Proteins -- chemistry KW - Sequence Homology, Amino Acid KW - Escherichia coli -- metabolism KW - Arginine -- chemistry KW - Models, Molecular KW - Dimerization KW - DNA Mutational Analysis KW - Serratia -- metabolism KW - Amino Acid Sequence KW - Hydrolysis KW - Binding Sites KW - Static Electricity KW - Histidine -- chemistry KW - Manganese -- chemistry KW - Protein Folding KW - Oxygen -- chemistry KW - RNA -- chemistry KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Catalysis KW - Endonucleases -- metabolism KW - Endonucleases -- chemistry KW - Anabaena -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68070603?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Structural+insights+into+the+mechanism+of+nuclease+A%2C+a+betabeta+alpha+metal+nuclease+from+Anabaena.&rft.au=Ghosh%2C+Mahua%3BMeiss%2C+Gregor%3BPingoud%2C+Alfred%3BLondon%2C+Robert+E%3BPedersen%2C+Lars+C&rft.aulast=Ghosh&rft.aufirst=Mahua&rft.date=2005-07-29&rft.volume=280&rft.issue=30&rft.spage=27990&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-07-25 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1ZM8; PDB N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene expression responses to DNA damage are altered in human aging and in Werner Syndrome. AN - 68083263; 15897889 AB - The accumulation of DNA damage and mutations is considered a major cause of cancer and aging. While it is known that DNA damage can affect changes in gene expression, transcriptional regulation after DNA damage is poorly understood. We characterized the expression of 6912 genes in human primary fibroblasts after exposure to three different kinds of cellular stress that introduces DNA damage: 4-nitroquinoline-1-oxide (4NQO), gamma-irradiation, or UV-irradiation. Each type of stress elicited damage specific gene expression changes of up to 10-fold. A total of 85 genes had similar changes in expression of 3-40-fold after all three kinds of stress. We examined transcription in cells from young and old individuals and from patients with Werner syndrome (WS), a segmental progeroid condition with a high incidence of cancer, and found various age-associated transcriptional changes depending upon the type of cellular stress. Compared to young individuals, both WS and old individuals had similarly aberrant transcriptional responses to gamma- and UV-irradiation, suggesting a role for Werner protein in stress-induced gene expression. Our results suggest that aberrant DNA damage-induced gene regulation may contribute to the aging process and the premature aging in WS. JF - Oncogene AU - Kyng, Kasper J AU - May, Alfred AU - Stevnsner, Tinna AU - Becker, Kevin G AU - Kølvrå, Steen AU - Bohr, Vilhelm A AD - Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. Y1 - 2005/07/28/ PY - 2005 DA - 2005 Jul 28 SP - 5026 EP - 5042 VL - 24 IS - 32 SN - 0950-9232, 0950-9232 KW - 4-nitroquinolone-1-oxide KW - 0 KW - Quinolones KW - 4-Nitroquinoline-1-oxide KW - 56-57-5 KW - Index Medicus KW - Fibroblasts -- drug effects KW - Ultraviolet Rays KW - Gamma Rays KW - Oligonucleotide Array Sequence Analysis KW - Genes, Immediate-Early -- radiation effects KW - Humans KW - Aged KW - Fibroblasts -- physiology KW - 4-Nitroquinoline-1-oxide -- pharmacology KW - Genes, Immediate-Early -- drug effects KW - Adult KW - Skin -- cytology KW - Fibroblasts -- radiation effects KW - Cell Line KW - Stress, Physiological KW - Quinolones -- pharmacology KW - Gene Expression Regulation -- radiation effects KW - Gene Expression Regulation -- drug effects KW - Werner Syndrome -- genetics KW - DNA Damage -- genetics KW - Aging -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68083263?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene&rft.atitle=Gene+expression+responses+to+DNA+damage+are+altered+in+human+aging+and+in+Werner+Syndrome.&rft.au=Kyng%2C+Kasper+J%3BMay%2C+Alfred%3BStevnsner%2C+Tinna%3BBecker%2C+Kevin+G%3BK%C3%B8lvr%C3%A5%2C+Steen%3BBohr%2C+Vilhelm+A&rft.aulast=Kyng&rft.aufirst=Kasper&rft.date=2005-07-28&rft.volume=24&rft.issue=32&rft.spage=5026&rft.isbn=&rft.btitle=&rft.title=Oncogene&rft.issn=09509232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-26 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of complex apurinic/apyrimidinic-site clustering associated with an authentic site-specific radiation-induced DNA double-strand break. AN - 68085584; 16024726 AB - Radiation lethality is largely attributed to radiation-induced DNA double-strand breaks (DSBs). A range of structural complexity is predicted for radiation-induced DSBs. However, this lesion has never been analyzed in isolation at the molecular level. To address this problem, we have created authentic site-specific radiation-induced DSBs in plasmid DNA by triplex-forming oligonucleotide-targeted 125I decay. No significant difference in DSB yield was observed after irradiation in the presence or absence of the radical scavenger DMSO, suggesting that DSB formation is a result of the direct effect of the radiation. A restriction fragment terminated by the DSB was isolated and probed with the Escherichia coli DNA repair enzyme endonuclease IV (endo IV), which recognizes apurinic/apyrimidinic (AP) sites. Enzymatic probing demonstrated clustering of AP sites within 10 bases of the 125I-targeted base in the DNA duplex. Our results suggest scavengeable radicals may not play a large role in the generation of AP sites associated with DSB formation, because at least 30% of all fragments have endo IV-sensitive sites, regardless of irradiation conditions. An internal control fragment recovered from the 125I linearized plasmid did not exhibit endo IV sensitivity in excess of that observed for a similar fragment recovered from an undamaged plasmid. Thus, AP site clustering proximal to the DSB resulted from the 125I decays responsible for DSB formation and was not due to untargeted background irradiation. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Datta, Kamal AU - Neumann, Ronald D AU - Winters, Thomas A AD - Nuclear Medicine Department, The Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07/26/ PY - 2005 DA - 2005 Jul 26 SP - 10569 EP - 10574 VL - 102 IS - 30 SN - 0027-8424, 0027-8424 KW - Escherichia coli Proteins KW - 0 KW - Iodine Radioisotopes KW - DNA KW - 9007-49-2 KW - Deoxyribonuclease IV (Phage T4-Induced) KW - EC 3.1.21.2 KW - endonuclease IV, E coli KW - DNA-(Apurinic or Apyrimidinic Site) Lyase KW - EC 4.2.99.18 KW - Dimethyl Sulfoxide KW - YOW8V9698H KW - Index Medicus KW - Escherichia coli Proteins -- metabolism KW - Base Sequence KW - DNA-(Apurinic or Apyrimidinic Site) Lyase -- metabolism KW - Deoxyribonuclease IV (Phage T4-Induced) -- metabolism KW - Plasmids -- genetics KW - DNA -- metabolism KW - Iodine Radioisotopes -- adverse effects KW - DNA -- radiation effects KW - DNA Damage -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68085584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Characterization+of+complex+apurinic%2Fapyrimidinic-site+clustering+associated+with+an+authentic+site-specific+radiation-induced+DNA+double-strand+break.&rft.au=Datta%2C+Kamal%3BNeumann%2C+Ronald+D%3BWinters%2C+Thomas+A&rft.aulast=Datta&rft.aufirst=Kamal&rft.date=2005-07-26&rft.volume=102&rft.issue=30&rft.spage=10569&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-29 N1 - Date created - 2005-07-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Acta Oncol. 1996;35(7):789-96 [9004754] Radiat Res. 2000 Mar;153(3):263-70 [10669547] Acta Oncol. 1996;35(7):817-23 [9004758] Nucleic Acids Res. 1997 Feb 15;25(4):883-7 [9016642] J Cell Biochem. 1997 Feb;64(2):258-72 [9027586] Int J Radiat Biol. 1997 Jul;72(1):91-100 [9246198] Antisense Nucleic Acid Drug Dev. 2000 Dec;10(6):443-52 [11198928] Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7426-30 [11404468] Radiat Res. 2001 Aug;156(2):158-66 [11448236] Radiat Res. 2001 Nov;156(5 Pt 2):577-83 [11604075] Int J Radiat Biol. 2002 Jun;78(6):457-66 [12065050] Nucleic Acids Res. 2002 Jul 1;30(13):2800-8 [12087163] J Am Chem Soc. 2002 Jul 31;124(30):8859-66 [12137539] Radiat Res. 2003 Feb;159(2):251-61 [12537531] Int J Radiat Biol. 2002 Dec;78(12):1095-102 [12556337] Anal Biochem. 2003 Jun 15;317(2):284-7 [12758272] Cold Spring Harb Symp Quant Biol. 2000;65:377-82 [12760053] Cancer Res. 2003 Sep 15;63(18):6008-15 [14522929] Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):489-505 [14565225] DNA Repair (Amst). 2004 Oct 5;3(10):1323-34 [15336627] Nucleic Acids Res. 2004;32(18):5609-20 [15494449] J Am Chem Soc. 1975 Apr 16;97(8):2277-8 [1133412] Radiat Res. 1981 May;86(2):185-95 [7015409] Science. 1981 Aug 21;213(4510):896-8 [7256283] J Biol Chem. 1982 Oct 10;257(19):11750-4 [7118909] J Biol Chem. 1983 Jan 25;258(2):711-3 [6822504] Radiat Res. 1985 Sep;103(3):383-92 [2994167] Radiat Res Suppl. 1985;8:S103-11 [3867077] Radiat Res. 1987 Jan;109(1):78-89 [3809393] Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 [2460825] Prog Nucleic Acid Res Mol Biol. 1988;35:95-125 [3065826] Int J Radiat Biol. 1991 Mar;59(3):625-42 [1672353] Int J Radiat Biol. 1994 Jan;65(1):7-17 [7905912] Int J Radiat Biol. 1994 Nov;66(5):427-32 [7983426] Nucleic Acids Res. 1994 Nov 25;22(23):4979-82 [7800489] Science. 1995 Feb 24;267(5201):1178-83 [7855601] Radiat Res. 1995 Oct;144(1):26-35 [7568768] Radiat Res. 2004 Dec;162(6):667-76 [15548117] Int J Radiat Biol. 1997 Sep;72(3):271-83 [9298107] Mutat Res. 1997 Sep;384(3):169-79 [9330613] Int J Radiat Biol. 1997 Oct;72(4):351-74 [9343102] J Nucl Med. 1998 Aug;39(8):1412-8 [9708519] C R Acad Sci III. 1999 Feb-Mar;322(2-3):89-101 [10196658] Chem Res Toxicol. 1999 Oct;12(10):917-23 [10525266] Int J Radiat Biol. 1999 Dec;75(12):1579-87 [10622264] Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):103-8 [10618378] Acta Oncol. 1996;35(7):797-801 [9004755] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ku86 preserves chromatin integrity in cells adapted to high NaCl. AN - 68084327; 16027367 AB - Cells adapted to high NaCl have many DNA breaks both in cell culture and in the renal inner medulla in vivo; yet they survive, function, and even proliferate. Here, we show that Ku86 is important for maintaining chromosomal integrity despite the continued presence of DNA breaks. The Ku heterodimer is part of DNA-dependent PK (DNA-PK), a complex that contributes by nonhomologous end joining to repair of double-strand breaks. We demonstrate that cells deficient in Ku86, but not cells deficient in DNA-PKcs (the catalytic subunit of DNA-PK), are hypersensitive to high NaCl as manifested by profound inhibition of proliferation, aberrant mitosis, and increased chromosomal fragmentation. Lower eukaryotes, including the soil nematode Caenorhabditis elegans, lack a DNA-PKcs homologue but are able to adapt to high NaCl. We show that cells of C. elegans adapted to high NaCl have many DNA breaks, similar to the mammalian cells adapted to high NaCl. Ku86 mutant C. elegans as well as C. elegans fed with cku86 dsRNA also display hypersensitivity to high NaCl, characterized by a reduced number of progeny and prolonged generation time in high NaCl. We propose that Ku86 ameliorates the effects of high NaCl-induced DNA breaks in adapted cells by supporting alignment of the broken ends of the DNA and thus maintaining integrity of the fragmented chromatin. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Dmitrieva, Natalia I AU - Celeste, Arkady AU - Nussenzweig, André AU - Burg, Maurice B AD - Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, and Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. dmitrien@nhlbi.nih.gov Y1 - 2005/07/26/ PY - 2005 DA - 2005 Jul 26 SP - 10730 EP - 10735 VL - 102 IS - 30 SN - 0027-8424, 0027-8424 KW - Antigens, Nuclear KW - 0 KW - Chromatin KW - DNA-Binding Proteins KW - Nuclear Proteins KW - Sodium Chloride KW - 451W47IQ8X KW - DNA-Activated Protein Kinase KW - EC 2.7.11.1 KW - Prkdc protein, mouse KW - Xrcc5 protein, mouse KW - EC 3.6.4.12 KW - Xrcc6 protein, human KW - Xrcc6 protein, mouse KW - Ku Autoantigen KW - EC 4.2.99.- KW - Index Medicus KW - Animals KW - Cytogenetic Analysis KW - Cricetulus KW - Reproduction -- drug effects KW - Humans KW - Mice KW - Blotting, Western KW - DNA-Activated Protein Kinase -- metabolism KW - Caenorhabditis elegans KW - RNA Interference KW - Nuclear Proteins -- metabolism KW - Cell Cycle -- drug effects KW - Cell Line KW - Cricetinae KW - DNA Damage KW - DNA-Binding Proteins -- genetics KW - Chromatin -- physiology KW - Antigens, Nuclear -- physiology KW - Antigens, Nuclear -- genetics KW - Chromatin -- genetics KW - DNA Repair -- genetics KW - DNA Repair -- physiology KW - Sodium Chloride -- toxicity KW - Antigens, Nuclear -- metabolism KW - Chromatin -- drug effects KW - DNA-Binding Proteins -- physiology KW - Adaptation, Physiological KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68084327?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Ku86+preserves+chromatin+integrity+in+cells+adapted+to+high+NaCl.&rft.au=Dmitrieva%2C+Natalia+I%3BCeleste%2C+Arkady%3BNussenzweig%2C+Andr%C3%A9%3BBurg%2C+Maurice+B&rft.aulast=Dmitrieva&rft.aufirst=Natalia&rft.date=2005-07-26&rft.volume=102&rft.issue=30&rft.spage=10730&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-29 N1 - Date created - 2005-07-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1999-2004 [11172065] Science. 2000 Dec 8;290(5498):1962-5 [11110662] Nature. 2001 Aug 9;412(6847):607-14 [11493912] Mutat Res. 2001 Sep 1;480-481:37-50 [11506797] Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):184-9 [11756692] Carcinogenesis. 2002 May;23(5):687-96 [12016139] Am J Physiol Renal Physiol. 2003 Aug;285(2):F266-74 [12684226] J Biol Chem. 2003 Oct 31;278(44):42729-32 [12912992] Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2317-22 [14983007] Am J Physiol Cell Physiol. 2004 Apr;286(4):C785-91 [14644776] Cell Cycle. 2004 May;3(5):561-3 [15107607] EMBO Rep. 2004 May;5(5):503-9 [15105825] Immunol Rev. 2004 Aug;200:132-41 [15242401] Mutat Res. 1983 Dec;112(6):313-27 [6197643] Radiat Res. 1993 Jun;134(3):349-54 [8316628] Am J Physiol. 1993 Sep;265(3 Pt 2):F416-24 [8214101] Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7623-7 [8052631] Science. 1994 Sep 2;265(5177):1442-5 [8073286] Science. 1994 Oct 14;266(5183):288-91 [7939667] Science. 1995 Feb 24;267(5201):1183-5 [7855602] Cell Prolif. 1995 Nov;28(11):571-9 [8555370] Nature. 1996 Aug 8;382(6591):551-5 [8700231] Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13588-93 [9391070] EMBO J. 1998 Jan 15;17(2):609-14 [9430651] Immunity. 1998 Sep;9(3):367-76 [9768756] Trends Biochem Sci. 1998 Oct;23(10):394-8 [9810228] Mol Cell Biol. 1999 May;19(5):3267-77 [10207052] Genes Dev. 1999 Apr 15;13(8):916-34 [10215620] Genes Cells. 1999 Feb;4(2):77-85 [10320474] Oncogene. 2005 Feb 3;24(6):949-61 [15592499] Nature. 2000 Mar 30;404(6777):510-4 [10761921] Genes Dev. 2000 Nov 15;14(22):2807-12 [11090128] Gene. 2001 Jan 24;263(1-2):103-12 [11223248] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A novel diacylglycerol-lactone shows marked selectivity in vitro among C1 domains of protein kinase C (PKC) isoforms alpha and delta as well as selectivity for RasGRP compared with PKCalpha. AN - 68054497; 15923197 AB - Although multiple natural products are potent ligands for the diacylglycerol binding C1 domain of protein kinase C (PKC), RasGRP, and related targets, the high conservation of C1 domains has impeded the development of selective ligands. We characterized here a diacylglycerol-lactone, 130C037, emerging from a combinatorial chemical synthetic strategy, which showed substantial selectivity. 130C037 gave shallow binding curves for PKC isoforms alpha, beta, gamma, delta, and epsilon, with apparent Ki values ranging from 340 nm for PKCalpha to 29 nm for PKCepsilon. When binding to isolated C1 domains of PKCalpha and -delta, 130C037 showed good affinity (Ki= 1.78 nm) only for deltaC1b, whereas phorbol 12,13-dibutyrate showed affinities within 10-fold for all. In LNCaP cells, 130C037 likewise selectively induced membrane translocation of deltaC1b. 130C037 bound intact RasGRP1 and RasGRP3 with Ki values of 3.5 and 3.8 nm, respectively, reflecting 8- and 90-fold selectivity relative to PKCepsilon and PKCalpha. By Western blot of Chinese hamster ovary cells, 130C037 selectively induced loss from the cytosol of RasGRP3 (ED50 = 286 nm), partial reduction of PKCepsilon (ED50 > 10 microm), and no effect on PKCalpha. As determined by confocal microscopy in LNCaP cells, 130C037 caused rapid translocation of RasGRP3, limited slow translocation of PKCepsilon, and no translocation of PKCalpha. Finally, 130C037 induced Erk phosphorylation in HEK-293 cells ectopically expressing RasGRP3 but not in control cells, whereas phorbol ester induced phosphorylation in both. The properties of 130C037 provide strong proof of principle for the feasibility of developing ligands with selectivity among C1 domain-containing therapeutic targets. JF - The Journal of biological chemistry AU - Pu, Yongmei AU - Perry, Nicholas A AU - Yang, Dazhi AU - Lewin, Nancy E AU - Kedei, Noemi AU - Braun, Derek C AU - Choi, Sung Hee AU - Blumberg, Peter M AU - Garfield, Susan H AU - Stone, James C AU - Duan, Dehui AU - Marquez, Victor E AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07/22/ PY - 2005 DA - 2005 Jul 22 SP - 27329 EP - 27338 VL - 280 IS - 29 SN - 0021-9258, 0021-9258 KW - 1,2-diacylglycerol KW - 0 KW - DNA-Binding Proteins KW - Diglycerides KW - Guanine Nucleotide Exchange Factors KW - Isoenzymes KW - Lactones KW - RASGRP1 protein, human KW - RASGRP3 protein, human KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - PRKCA protein, human KW - EC 2.7.11.13 KW - PRKCD protein, human KW - Protein Kinase C KW - Protein Kinase C-alpha KW - Protein Kinase C-delta KW - Index Medicus KW - Animals KW - Guanine Nucleotide Exchange Factors -- metabolism KW - Humans KW - Protein Binding KW - Binding Sites KW - Phorbol 12,13-Dibutyrate -- pharmacology KW - Phosphorylation -- drug effects KW - Cell Membrane -- metabolism KW - Cell Line KW - Protein Transport KW - DNA-Binding Proteins -- metabolism KW - Protein Kinase C -- metabolism KW - Diglycerides -- pharmacology KW - Protein Kinase C -- chemistry KW - Lactones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68054497?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=A+novel+diacylglycerol-lactone+shows+marked+selectivity+in+vitro+among+C1+domains+of+protein+kinase+C+%28PKC%29+isoforms+alpha+and+delta+as+well+as+selectivity+for+RasGRP+compared+with+PKCalpha.&rft.au=Pu%2C+Yongmei%3BPerry%2C+Nicholas+A%3BYang%2C+Dazhi%3BLewin%2C+Nancy+E%3BKedei%2C+Noemi%3BBraun%2C+Derek+C%3BChoi%2C+Sung+Hee%3BBlumberg%2C+Peter+M%3BGarfield%2C+Susan+H%3BStone%2C+James+C%3BDuan%2C+Dehui%3BMarquez%2C+Victor+E&rft.aulast=Pu&rft.aufirst=Yongmei&rft.date=2005-07-22&rft.volume=280&rft.issue=29&rft.spage=27329&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-07-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Heart rate variability under acute simulated microgravity during daytime waking state and nocturnal sleep: Comparison of horizontal and 6 degree head- down bed rest AN - 17392863; 6483019 AB - This study examined the acute effect of cephalad fluid shift under simulated microgravity on heart rate variability (HRV) during both daytime waking state and nocturnal sleep. Seven healthy male volunteers (21-31 years) underwent a series of experiments involving 6 degree head-down bed rest (HD) for 3 days. A control experiment on the same subjects was conducted under horizontal bed rest (HZ) in the same series. HRV from electrocardiogram signals was periodically calculated by the MemCalc method during daytime on the first and second days of both conditions. Nocturnal sleep on the first night of bed rest was monitored by polysomnography. HRV during stage 2 sleep and REM sleep were assessed in the former and latter halves of the sleep period time. Nocturnal sleep architecture under both conditions was normal, but a slight decrease in stage 4 sleep and an increase in the number of arousals occurred under HD. On both the first and second days, HRV during the daytime did not differ between HZ and HD. In contrast, high frequency components in HRV during sleep stage 2 were significantly higher in the latter half of sleep under HD than under HZ, although there were no differences in the ratio of low frequency to high frequency components during both stage 2 and the REM stage between the conditions. These results suggest that the acute effect of the cephalad fluid shift on cardiac autonomic nervous activity might be affected by the sleep/wake state modulating the dominance between sympathetic and parasympathetic nervous activity. JF - Neuroscience Letters AU - Mizuno, Koh AU - Inoue, Yuichi AU - Tanaka, Hideki AU - Komada, Yoko AU - Saito, Hidetomo AU - Mishima, Kazuo AU - Shirakawa, Shuichiro AD - Geriatric Mental Health, National Institute of Mental Health, National Center of Neurology and Psychiatry, Kohnodai 1-7-3, Ichikawa, Chiba 272- 0827, Japan Y1 - 2005/07/22/ PY - 2005 DA - 2005 Jul 22 SP - 115 EP - 120 VL - 383 IS - 1-2 SN - 0304-3940, 0304-3940 KW - Physical Education Index; CSA Neurosciences Abstracts KW - Sleep (REM) KW - Autonomic nervous system KW - Parasympathetic nervous system KW - Gravity KW - Arousal KW - Heart rate KW - Sympathetic nervous system KW - Health KW - Sleep and wakefulness KW - EKG KW - Dominance KW - Microgravity KW - Nervous system KW - Daytime KW - Sleep KW - Electrocardiology KW - Immobilization KW - N3 11140:Sleep and sleep disorders KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17392863?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+Letters&rft.atitle=Heart+rate+variability+under+acute+simulated+microgravity+during+daytime+waking+state+and+nocturnal+sleep%3A+Comparison+of+horizontal+and+6+degree+head-+down+bed+rest&rft.au=Mizuno%2C+Koh%3BInoue%2C+Yuichi%3BTanaka%2C+Hideki%3BKomada%2C+Yoko%3BSaito%2C+Hidetomo%3BMishima%2C+Kazuo%3BShirakawa%2C+Shuichiro&rft.aulast=Mizuno&rft.aufirst=Koh&rft.date=2005-07-22&rft.volume=383&rft.issue=1-2&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Neuroscience+Letters&rft.issn=03043940&rft_id=info:doi/10.1016%2Fj.neulet.2005.03.058 LA - English DB - Physical Education Index N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Nervous system; Arousal; Gravity; Sleep; Heart rate; Health; Electrocardiology; Immobilization; Dominance; Microgravity; Autonomic nervous system; Sleep (REM); Daytime; Parasympathetic nervous system; Sympathetic nervous system; Sleep and wakefulness; EKG DO - http://dx.doi.org/10.1016/j.neulet.2005.03.058 ER - TY - JOUR T1 - Gadolinium-Rhodamine Nanoparticles for Cell Labeling and Tracking via Magnetic Resonance and Optical Imaging AN - 19426154; 6486317 AB - A novel dual-labeled nanoparticle for use in labeling and tracking cells in vivo is described. We report the construction and characterization of these gadolinium-rhodamine nanoparticles. These particles are constructed from lipid monomers with diacetylene bonds that are sonicated and photolyzed to form polymerized nanoparticles. Cells are efficiently labeled with these nanoparticles. We have inoculated labeled tumor cells subcutaneouosly into the flanks of C3H mice and have been able to image these labeled tumor cells via MRI and optical imaging. Furthermore, the labeled tumor cells can be visualized via fluorescent microscopy after tissue biopsy. Our results suggest that these nanoparticles could be used to track cells in vivo. This basic platform can be modified with different fluorophores and targeting agents for studying metastisic cell, stem cell, and immune cell trafficking among other applications. JF - Bioconjugate Chemistry AU - Vuu, K AU - Xie, J AU - McDonald, MA AU - Bernardo, M AU - Hunter, F AU - Zhang, Y AU - Li, K AU - Bednarski, M AU - Guccione, S AD - Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, 4000 Jones Bridge Road, Chevy Chase, Maryland, 20815-6789, USA Y1 - 2005/07/20/ PY - 2005 DA - 2005 Jul 20 SP - 995 EP - 999 VL - 16 IS - 4 SN - 1043-1802, 1043-1802 KW - Biotechnology and Bioengineering Abstracts KW - Monomers KW - Stem cells KW - Lipids KW - Magnetic resonance imaging KW - N.M.R. KW - Biopsy KW - fluorophores KW - Tumor cells KW - nanoparticles KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19426154?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioconjugate+Chemistry&rft.atitle=Gadolinium-Rhodamine+Nanoparticles+for+Cell+Labeling+and+Tracking+via+Magnetic+Resonance+and+Optical+Imaging&rft.au=Vuu%2C+K%3BXie%2C+J%3BMcDonald%2C+MA%3BBernardo%2C+M%3BHunter%2C+F%3BZhang%2C+Y%3BLi%2C+K%3BBednarski%2C+M%3BGuccione%2C+S&rft.aulast=Vuu&rft.aufirst=K&rft.date=2005-07-20&rft.volume=16&rft.issue=4&rft.spage=995&rft.isbn=&rft.btitle=&rft.title=Bioconjugate+Chemistry&rft.issn=10431802&rft_id=info:doi/10.1021%2Fbc050085z LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - nanoparticles; Tumor cells; Stem cells; Lipids; Biopsy; Monomers; N.M.R.; fluorophores; Magnetic resonance imaging DO - http://dx.doi.org/10.1021/bc050085z ER - TY - CPAPER T1 - Dynamical Effects of Diffusive Cell Coupling on the Behavior of Pancreatic Beta-Cells. T2 - 6th European Conference on Mathematical and Theoretical Biology (ECMTB 2005) AN - 40006667; 3973850 JF - 6th European Conference on Mathematical and Theoretical Biology (ECMTB 2005) AU - Tsaneva-Atanasova, K AU - Sherman, A AU - Satin, L S AU - Nunemaker, C S AU - Zhang, M Y1 - 2005/07/18/ PY - 2005 DA - 2005 Jul 18 KW - Pancreas KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40006667?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+European+Conference+on+Mathematical+and+Theoretical+Biology+%28ECMTB+2005%29&rft.atitle=Dynamical+Effects+of+Diffusive+Cell+Coupling+on+the+Behavior+of+Pancreatic+Beta-Cells.&rft.au=Tsaneva-Atanasova%2C+K%3BSherman%2C+A%3BSatin%2C+L+S%3BNunemaker%2C+C+S%3BZhang%2C+M&rft.aulast=Tsaneva-Atanasova&rft.aufirst=K&rft.date=2005-07-18&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+European+Conference+on+Mathematical+and+Theoretical+Biology+%28ECMTB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.ecmtb05.org/php/schedule.php?listof=sessions LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Arfs, Phosphoinositides and Membrane Traffic T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 40041722; 3971636 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Donaldson, Julie G Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - phosphoinositides KW - membrane trafficking KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=Arfs%2C+Phosphoinositides+and+Membrane+Traffic&rft.au=Donaldson%2C+Julie+G&rft.aulast=Donaldson&rft.aufirst=Julie&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Most Infectious Scrapie Particle and Scrapie Prion Protein Trafficking in Neurons T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 40016859; 3971742 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Silveira, Jay AU - Magalhaes, Ana Cristina AU - Baron, Gerald S AU - Lee, Kil Sun AU - Prado, Marco AM AU - Caughey, Byron Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - Prion protein KW - Particulates KW - Neurons KW - Protein transport KW - Scrapie KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40016859?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=The+Most+Infectious+Scrapie+Particle+and+Scrapie+Prion+Protein+Trafficking+in+Neurons&rft.au=Silveira%2C+Jay%3BMagalhaes%2C+Ana+Cristina%3BBaron%2C+Gerald+S%3BLee%2C+Kil+Sun%3BPrado%2C+Marco+AM%3BCaughey%2C+Byron&rft.aulast=Silveira&rft.aufirst=Jay&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Unravelling the Reactions of Nitric Oxide, Nitrite and Haemoglobin T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 39988728; 3971713 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Gladwin, Mark T Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - Hemoglobin KW - Nitrite KW - Nitric oxide KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39988728?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=Unravelling+the+Reactions+of+Nitric+Oxide%2C+Nitrite+and+Haemoglobin&rft.au=Gladwin%2C+Mark+T&rft.aulast=Gladwin&rft.aufirst=Mark&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Pharmacological Stimulation of Beta2 Adrenergic Receptors(AR) Enhances Therapeutic Effectiveness of Beta1 Ar Blockade in Rodent Dilated Ischemic Cardiomyopathy T2 - 12th World Congress on Heart Disease - New Trends in Research, Diagnosis and Treatment AN - 40014011; 3942728 JF - 12th World Congress on Heart Disease - New Trends in Research, Diagnosis and Treatment AU - Ahmet, I AU - Lakatta, E G AU - Talan, M I Y1 - 2005/07/16/ PY - 2005 DA - 2005 Jul 16 KW - Cardiomyopathy KW - Ischemia KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40014011?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=12th+World+Congress+on+Heart+Disease+-+New+Trends+in+Research%2C+Diagnosis+and+Treatment&rft.atitle=Pharmacological+Stimulation+of+Beta2+Adrenergic+Receptors%28AR%29+Enhances+Therapeutic+Effectiveness+of+Beta1+Ar+Blockade+in+Rodent+Dilated+Ischemic+Cardiomyopathy&rft.au=Ahmet%2C+I%3BLakatta%2C+E+G%3BTalan%2C+M+I&rft.aulast=Ahmet&rft.aufirst=I&rft.date=2005-07-16&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=12th+World+Congress+on+Heart+Disease+-+New+Trends+in+Research%2C+Diagnosis+and+Treatment&rft.issn=&rft_id=info:doi/ L2 - http://www.cardiologyonline.com/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Evaluation of radiation-induced oral mucositis by optical coherence tomography. AN - 68056535; 16033826 AB - Optical coherence tomography (OCT) imaging was evaluated to determine if radiation-induced mucosal damage could be noninvasively monitored in real time and correlated with histopathologic findings. Female C3H mice, ages 7 to 9 weeks, four per group, were immobilized in a custom-made Lucite jig and received 0, 15, 22.5, and 25 Gy in a single fraction to their oral cavity. OCT images were acquired of proximal, middle, and distal aspects of the dorsum of the tongue on days 0, 1, 3, 5, and 7 post-irradiation. Animals were sacrificed on day 7 and samples taken for histologic evaluation. OCT images were visually examined and also quantified by image analysis and compared with histologic findings. Tongues removed 7 days post-irradiation showed no visible damage; however, upon staining with toluidine blue, ulcers at the base of the tongue became visible (100% for 25 Gy, 75% after 22.5 Gy, and 0% after 15 Gy). Visual inspection of OCT images qualitatively compared with histologic findings and quantitative image analysis of the OCT images (effective light penetration depth) revealed significant changes 7 days post-irradiation compared with unirradiated controls for the base of the tongue. OCT allows for direct noninvasive real-time acquisition of digitally archivable images of oral mucosa and can detect radiation-induced changes in the mucosa before visual manifestation. OCT may be a useful technique to quantify subclinical radiation-induced mucosal injury in experimental chemoradiation clinical trials. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Muanza, Thierry M AU - Cotrim, Ana P AU - McAuliffe, Mathew AU - Sowers, Anastasia L AU - Baum, Bruce J AU - Cook, John A AU - Feldchtein, Felix AU - Amazeen, Paul AU - Coleman, C Norman AU - Mitchell, James B AD - Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 5121 EP - 5127 VL - 11 IS - 14 SN - 1078-0432, 1078-0432 KW - Index Medicus KW - Severity of Illness Index KW - Sensitivity and Specificity KW - Animals KW - Mouth Mucosa KW - Disease Models, Animal KW - Mice KW - Female KW - Radiation Injuries -- veterinary KW - Stomatitis -- veterinary KW - Stomatitis -- etiology KW - Stomatitis -- diagnosis KW - Tomography, Optical Coherence KW - Radiation Injuries -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Evaluation+of+radiation-induced+oral+mucositis+by+optical+coherence+tomography.&rft.au=Muanza%2C+Thierry+M%3BCotrim%2C+Ana+P%3BMcAuliffe%2C+Mathew%3BSowers%2C+Anastasia+L%3BBaum%2C+Bruce+J%3BCook%2C+John+A%3BFeldchtein%2C+Felix%3BAmazeen%2C+Paul%3BColeman%2C+C+Norman%3BMitchell%2C+James+B&rft.aulast=Muanza&rft.aufirst=Thierry&rft.date=2005-07-15&rft.volume=11&rft.issue=14&rft.spage=5121&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-07 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. AN - 68045834; 16024603 AB - Programmed cell death 4 (Pdcd4) is a novel repressor of in vitro transformation. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A, a component of the translation initiation complex. To ascertain whether Pdcd4 suppresses tumor development in vivo, we have generated transgenic mice that overexpress Pdcd4 in the epidermis (K14-Pdcd4). K14-regulated Pdcd4 expression caused a neonatal short-hair phenotype due to early catagen entry compared with matched wild-type siblings. In response to the 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA) mouse skin carcinogenesis protocol, K14-Pdcd4 mice showed significant reductions in papilloma formation, carcinoma incidence, and papilloma-to-carcinoma conversion frequency compared with wild-type mice. The translational efficiency of an mRNA engineered to form a structured 5' untranslated region (UTR) was attenuated in primary keratinocytes when Pdcd4 was overexpressed. Pdcd4 inhibited by 46% TPA-induced activator protein-1 (AP-1)-dependent transcription, an event required for tumorigenesis. CDK4 and ornithine decarboxylase (ODC) are candidates for Pdcd4-regulated translation as their mRNAs contain 5'structured UTRs. In K14-Pdcd4 primary keratinocytes expressing activated Ha-Ras to mimic DMBA-initiated epidermis, ODC and CDK4 protein levels were decreased by 40% and 46%, respectively. Expression of a protein encoded by 5' unstructured mRNA showed no change. These results extend to an in vivo model the observations that Pdcd4 inhibits both translation initiation and AP-1 activation while decreasing benign tumor development and malignant progression. The K14-Pdcd4 mice seem to validate translation initiation as a novel target for cancer prevention. JF - Cancer research AU - Jansen, Aaron P AU - Camalier, Corinne E AU - Colburn, Nancy H AD - Gene Regulation Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA. ajansen@ncifcrf.gov Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 6034 EP - 6041 VL - 65 IS - 14 SN - 0008-5472, 0008-5472 KW - 5' Untranslated Regions KW - 0 KW - Apoptosis Regulatory Proteins KW - Pdcd4 protein, mouse KW - RNA, Messenger KW - RNA-Binding Proteins KW - Transcription Factor AP-1 KW - Luciferases KW - EC 1.13.12.- KW - Index Medicus KW - Protein Biosynthesis KW - Animals KW - Transcription Factor AP-1 -- antagonists & inhibitors KW - Epidermis -- metabolism KW - Disease Progression KW - Mice KW - RNA, Messenger -- genetics KW - Mice, Transgenic KW - Transcription Factor AP-1 -- physiology KW - Transcriptional Activation KW - RNA, Messenger -- biosynthesis KW - Luciferases -- biosynthesis KW - Promoter Regions, Genetic KW - Cytoplasm -- metabolism KW - Luciferases -- antagonists & inhibitors KW - Epidermis -- pathology KW - Luciferases -- genetics KW - Female KW - Papilloma -- pathology KW - Papilloma -- prevention & control KW - RNA-Binding Proteins -- genetics KW - RNA-Binding Proteins -- physiology KW - Skin Neoplasms -- pathology KW - Carcinoma, Squamous Cell -- metabolism KW - Papilloma -- genetics KW - Skin Neoplasms -- prevention & control KW - Skin Neoplasms -- metabolism KW - Skin Neoplasms -- genetics KW - RNA-Binding Proteins -- biosynthesis KW - Carcinoma, Squamous Cell -- pathology KW - Carcinoma, Squamous Cell -- genetics KW - Carcinoma, Squamous Cell -- prevention & control KW - Papilloma -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68045834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Epidermal+expression+of+the+translation+inhibitor+programmed+cell+death+4+suppresses+tumorigenesis.&rft.au=Jansen%2C+Aaron+P%3BCamalier%2C+Corinne+E%3BColburn%2C+Nancy+H&rft.aulast=Jansen&rft.aufirst=Aaron&rft.date=2005-07-15&rft.volume=65&rft.issue=14&rft.spage=6034&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-14 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of claudin-3 at threonine 192 by cAMP-dependent protein kinase regulates tight junction barrier function in ovarian cancer cells. AN - 68020519; 15905176 AB - Claudins are integral membrane proteins essential in the formation and function of tight junctions (TJs). Disruption of TJs, which have essential roles in cell permeability and polarity, is thought to contribute to epithelial tumorigenesis. Claudin-3 and -4 are frequently overexpressed in ovarian cancer, but the molecular pathways involved in the regulation of these proteins are unclear. Interestingly, several studies have demonstrated a role for phosphorylation in the regulation of TJ complexes, although evidence for claudin phosphorylation is scarce. Here, we showed that claudin-3 and -4 can be phosphorylated in ovarian cancer cells. In vitro phosphorylation assays using glutathione S-transferase fusion constructs demonstrated that the C terminus of claudin-3 is an excellent substrate for cAMP-dependent protein kinase (PKA). Using site-directed mutagenesis, we identified a PKA phosphorylation site at amino acid 192 in the C terminus of claudin-3. Overexpression of the protein containing a T192D mutation, mimicking the phosphorylated state, resulted in a decrease in TJ strength in ovarian cancer cell line OVCA433. Our results suggest that claudin-3 phosphorylation by PKA, a kinase frequently activated in ovarian cancer, may provide a mechanism for the disruption of TJs in this cancer. In addition, our findings may have general implications for the regulation of TJs in normal epithelial cells. JF - The Journal of biological chemistry AU - D'Souza, Theresa AU - Agarwal, Rachana AU - Morin, Patrice J AD - Laboratory of Cellular and Molecular Biology, Gerontology Research Center, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 26233 EP - 26240 VL - 280 IS - 28 SN - 0021-9258, 0021-9258 KW - CLDN3 protein, human KW - 0 KW - CLDN4 protein, human KW - Claudin-3 KW - Claudin-4 KW - Enzyme Inhibitors KW - Membrane Proteins KW - Recombinant Fusion Proteins KW - Recombinant Proteins KW - Threonine KW - 2ZD004190S KW - Glutathione Transferase KW - EC 2.5.1.18 KW - Cyclic AMP-Dependent Protein Kinases KW - EC 2.7.11.11 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Recombinant Fusion Proteins -- chemistry KW - Calcium -- metabolism KW - Microscopy, Fluorescence KW - Recombinant Fusion Proteins -- metabolism KW - Permeability KW - Phosphorylation KW - Recombinant Proteins -- chemistry KW - Time Factors KW - Immunoblotting KW - Electric Impedance KW - Immunoprecipitation KW - Cell Line, Tumor KW - Electrophysiology KW - Binding Sites KW - Transfection KW - Models, Genetic KW - Enzyme Inhibitors -- pharmacology KW - Protein Structure, Tertiary KW - Immunohistochemistry KW - Mutation KW - Female KW - Cell Line KW - Cyclic AMP-Dependent Protein Kinases -- metabolism KW - Ovarian Neoplasms -- metabolism KW - Threonine -- chemistry KW - Cyclic AMP-Dependent Protein Kinases -- physiology KW - Membrane Proteins -- chemistry KW - Membrane Proteins -- metabolism KW - Glutathione Transferase -- metabolism KW - Cyclic AMP-Dependent Protein Kinases -- chemistry KW - Tight Junctions -- chemistry KW - Membrane Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68020519?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Phosphorylation+of+claudin-3+at+threonine+192+by+cAMP-dependent+protein+kinase+regulates+tight+junction+barrier+function+in+ovarian+cancer+cells.&rft.au=D%27Souza%2C+Theresa%3BAgarwal%2C+Rachana%3BMorin%2C+Patrice+J&rft.aulast=D%27Souza&rft.aufirst=Theresa&rft.date=2005-07-15&rft.volume=280&rft.issue=28&rft.spage=26233&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-12 N1 - Date created - 2005-07-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The Ins(1,3,4)P3 5/6-kinase/Ins(3,4,5,6)P4 1-kinase is not a protein kinase. AN - 67997088; 15762844 AB - Among inositol phosphate kinases, Ins(3,4,5,6)P4 1-kinase has been considered to be an outsider with disparate sequence, a proclaimed capacity to also phosphorylate proteins and apparent 1-phosphatase activity. Such multifunctionality, coupled with ignorance of its operational domains, complicates any mechanistic rationale behind literature reports that Ins(3,4,5,6)P4 1-kinase regulates apoptosis, salt and fluid secretion, and transcription. We have expressed poly(His)-tagged human Ins(3,4,5,6)P4 1-kinase in Sf9 insect cells and purified the enzyme using Ni-agarose chromatography. Protein kinase activity was eluted from the Ni-agarose column, but this did not co-elute with the Ins(3,4,5,6)P4 1-kinase, indicating that the protein kinase and inositol kinase activities belong to separate proteins. To pursue this conclusion, we prepared catalytically inactive mutants of the Ins(3,4,5,6)P4 1-kinase by identifying and targeting the ATP-binding site. Our strategy was based on sequence alignments suggesting homology of the Ins(3,4,5,6)P4 1-kinase with ATP-grasp metabolic enzymes. Individual mutation of four candidate MgATP-binding participants, Lys157, Asp281, Asp295 and Asn297, severely compromised Ins(3,4,5,6)P4 1-kinase activity. Yet, these mutations did not affect the protein kinase activity. We conclude that the Ins(3,4,5,6)P4 1-kinase is not a protein kinase, contrary to earlier reports [e.g. Wilson, Sun, Cao and Majerus (2001) J. Biol. Chem. 276, 40998-41004]. Elimination of protein kinase activity from the enzyme's repertoire and recognition of its ATP-grasp homology together indicate that structural, functional and catalytic relationships between Ins(3,4,5,6)P4 1-kinase and other inositol phosphate kinases are closer than previously thought [Gonzalez, Schell, Letcher, Veprintsev, Irvine and Williams (2004) Mol. Cell 15, 689-701]. JF - The Biochemical journal AU - Qian, Xun AU - Mitchell, Jennifer AU - Wei, Sung-Jen AU - Williams, Jason AU - Petrovich, Robert M AU - Shears, Stephen B AD - Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHSS, Research Triangle Park, PO Box 12233, NC 27709, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 389 EP - 395 VL - 389 KW - Recombinant Proteins KW - 0 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Phosphotransferases (Alcohol Group Acceptor) KW - EC 2.7.1.- KW - inositol-tetrakisphosphate 1-kinase KW - myo-inositol-trisphosphate 6-kinase KW - EC 2.7.1.134 KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Animals KW - Conserved Sequence KW - Humans KW - Adenosine Triphosphate -- metabolism KW - Amino Acid Sequence KW - Substrate Specificity KW - Protein Structure, Tertiary KW - Cell Line KW - Catalysis KW - Binding Sites KW - Phosphotransferases (Alcohol Group Acceptor) -- chemistry KW - Phosphotransferases (Alcohol Group Acceptor) -- metabolism KW - Phosphotransferases (Alcohol Group Acceptor) -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67997088?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Biochemical+journal&rft.atitle=The+Ins%281%2C3%2C4%29P3+5%2F6-kinase%2FIns%283%2C4%2C5%2C6%29P4+1-kinase+is+not+a+protein+kinase.&rft.au=Qian%2C+Xun%3BMitchell%2C+Jennifer%3BWei%2C+Sung-Jen%3BWilliams%2C+Jason%3BPetrovich%2C+Robert+M%3BShears%2C+Stephen+B&rft.aulast=Qian&rft.aufirst=Xun&rft.date=2005-07-15&rft.volume=389&rft.issue=&rft.spage=389&rft.isbn=&rft.btitle=&rft.title=The+Biochemical+journal&rft.issn=1470-8728&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-21 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 2003 Oct 31;278(44):43645-53 [12925536] Cell. 2003 Sep 19;114(6):663-71 [14505567] Mol Cell. 2004 Sep 10;15(5):689-701 [15350214] Mol Cell. 2004 Sep 10;15(5):703-11 [15350215] Science. 1994 Oct 21;266(5184):439-43 [7939684] Biochemistry. 1995 Mar 7;34(9):2768-76 [7893688] J Biol Chem. 1996 Jun 14;271(24):14092-7 [8662902] Curr Opin Struct Biol. 1996 Jun;6(3):386-94 [8804825] Biochemistry. 1996 Nov 12;35(45):14352-61 [8916922] Biochemistry. 1996 Nov 12;35(45):14362-9 [8916923] Protein Sci. 1997 Dec;6(12):2639-43 [9416615] Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13597-602 [9811845] Biochemistry. 1999 Feb 23;38(8):2347-57 [10029528] Biochemistry. 1999 Mar 16;38(11):3393-400 [10079084] J Biol Chem. 2000 May 26;275(21):16183-90 [10821865] J Mol Biol. 2000 Jun 2;299(2):499-520 [10860755] Biochem J. 2000 Nov 1;351 Pt 3:551-5 [11042108] Biochemistry. 2000 Dec 26;39(51):15895-900 [11123916] Nat Rev Mol Cell Biol. 2001 May;2(5):327-38 [11331907] J Biol Chem. 2001 Jul 6;276(27):24991-6 [11346647] Trends Cell Biol. 2001 Oct;11(10):420-6 [11567875] J Biol Chem. 2001 Nov 2;276(44):40998-1004 [11533064] Curr Biol. 2002 Mar 19;12(6):477-82 [11909533] J Mol Biol. 2002 Jul 19;320(4):855-81 [12095261] J Biol Chem. 2002 Jul 26;277(30):26717-20 [12055181] J Biol Chem. 2002 Nov 15;277(46):43836-43 [12223481] J Biol Chem. 2002 Nov 29;277(48):45759-64 [12324474] EMBO J. 2003 Mar 17;22(6):1302-12 [12628923] Trends Genet. 2003 Aug;19(8):415-7 [12902157] Biochem J. 2004 Jan 15;377(Pt 2):265-80 [14567754] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization. AN - 67996919; 15743274 AB - All 20 cysteine residues are accessible to disulphide reagents in the tubulin dimer, whereas only four are accessible in taxol-stabilized microtubules. Reaction rates with disulphide reagents are a function of the reagent, are decreased by G nucleotides, and increased with increase in pH and urea. With transient (stop-flow) kinetics, DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)] and 2,2'-dithiodipyridine progress curves cannot be fitted by the sum of exponential terms based only on classes of cysteines. The mixed disulphide products react further to form both intra- and intermonomer disulphide bonds that can be reversed by reducing agents. With MMTS (methyl methanethiosulphonate) or ODNB (n-octyl-dithio-2-nitrobenzoate), virtually no protein-protein disulphide bonds are formed and the ODNB reaction can be given as the sum of three exponential terms with pseudo-first-order rate constants of 0.206, 0.069 and 0.010 s(-1) at pH 6.5, suggesting three classes of thiol reactivities. Limited cysteine substitution leads to only small changes in tryptophan or CD spectra, whereas complete substitution leads to loss of the helix content. MMTS-induced loss of SH groups leads to progressive increases in the critical concentration and loss of polymerization competence that can be reversed by assembly promoters such as higher protein concentration, taxol or high ionic strength. Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles. JF - The Biochemical journal AU - Britto, P J AU - Knipling, Leslie AU - McPhie, Peter AU - Wolff, J AD - Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 549 EP - 558 VL - 389 KW - Biopolymers KW - 0 KW - Disulfides KW - Nitrobenzoates KW - Sulfhydryl Compounds KW - Tubulin KW - Guanosine Diphosphate KW - 146-91-8 KW - n-octyl-5-dithio-2-nitrobenzoic acid KW - 146019-29-6 KW - thionitrobenzoic acid KW - 15139-21-6 KW - 2,2'-dipyridyl disulfide KW - 2127-03-9 KW - methyl methanethiosulfonate KW - 2949-92-0 KW - 2,2'-Dipyridyl KW - 551W113ZEP KW - Guanosine Triphosphate KW - 86-01-1 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Dithionitrobenzoic Acid KW - 9BZQ3U62JX KW - Methyl Methanesulfonate KW - AT5C31J09G KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Molecular Structure KW - Biopolymers -- chemistry KW - Biopolymers -- metabolism KW - Cysteine -- metabolism KW - Guanosine Diphosphate -- metabolism KW - Protein Denaturation KW - 2,2'-Dipyridyl -- analogs & derivatives KW - Amino Acid Sequence KW - Methyl Methanesulfonate -- analogs & derivatives KW - Guanosine Triphosphate -- metabolism KW - Methyl Methanesulfonate -- chemistry KW - Cysteine -- chemistry KW - Dithionitrobenzoic Acid -- chemistry KW - Kinetics KW - Adenosine Triphosphate -- metabolism KW - 2,2'-Dipyridyl -- chemistry KW - Nitrobenzoates -- chemistry KW - Disulfides -- chemistry KW - Sulfhydryl Compounds -- metabolism KW - Sulfhydryl Compounds -- chemistry KW - Tubulin -- chemistry KW - Tubulin -- metabolism KW - Disulfides -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67996919?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Biochemical+journal&rft.atitle=Thiol-disulphide+interchange+in+tubulin%3A+kinetics+and+the+effect+on+polymerization.&rft.au=Britto%2C+P+J%3BKnipling%2C+Leslie%3BMcPhie%2C+Peter%3BWolff%2C+J&rft.aulast=Britto&rft.aufirst=P&rft.date=2005-07-15&rft.volume=389&rft.issue=&rft.spage=549&rft.isbn=&rft.btitle=&rft.title=The+Biochemical+journal&rft.issn=1470-8728&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-21 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Anal Biochem. 1979 Apr 1;94(1):75-81 [37780] Eur J Biochem. 1976 Sep;68(1):313-9 [964268] J Cell Biol. 1981 Apr;89(1):45-53 [7228899] Biochemistry. 1981 Jul 21;20(15):4437-44 [7284333] Biochim Biophys Acta. 1981 Nov 30;671(1):71-7 [7306573] Biochemistry. 1983 Mar 29;22(7):1567-72 [6849867] Biochim Biophys Acta. 1989 Jun 13;996(1-2):110-5 [2736254] Ital J Biochem. 1989 Mar-Apr;38(2):83-90 [2745039] Oxf Surv Eukaryot Genes. 1984;1:36-60 [6400775] Biochemistry. 1989 Nov 14;28(23):9143-52 [2605248] Adv Enzymol Relat Areas Mol Biol. 1990;63:69-172 [2407068] Biochim Biophys Acta. 1991 Jan 29;1076(2):289-97 [1998728] Pharmacol Ther. 1991;49(1-2):133-52 [1852786] J Biol Chem. 1993 Jan 5;268(1):127-32 [8416920] Anal Biochem. 1993 Feb 1;208(2):357-62 [8452233] Biochemistry. 1994 Nov 1;33(43):12868-78 [7947693] Protein Sci. 1995 Nov;4(11):2411-23 [8563639] Biochemistry. 1996 May 7;35(18):5910-20 [8639553] Nat Struct Biol. 1997 Jun;4(6):450-5 [9187652] Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3661-6 [9520422] J Biol Chem. 1998 Jul 24;273(30):19198-206 [9668107] Eur J Biochem. 2000 Jun;267(12):3469-76 [10848962] Biochemistry. 2001 Jul 31;40(30):8834-41 [11467944] J Mol Biol. 2001 Nov 9;313(5):1045-57 [11700061] Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1353-8 [11805304] Arch Biochem Biophys. 2002 Feb 15;398(2):213-20 [11831852] J Biol Chem. 2002 Aug 9;277(32):29018-27 [12023292] Arch Biochem Biophys. 2002 Oct 15;406(2):229-40 [12361711] Anal Biochem. 2003 Jan 15;312(2):224-7 [12531209] Biochim Biophys Acta. 2003 Apr 8;1631(3):239-45 [12668175] Biochem Biophys Res Commun. 2004 Feb 6;314(2):555-60 [14733943] Free Radic Biol Med. 2004 Feb 15;36(4):497-506 [14975452] J Biol Chem. 2004 May 21;279(21):21749-58 [15031298] J Biol Chem. 1967 Jun 10;242(11):2709-18 [6027244] Biochem J. 1973 May;133(1):67-80 [4721623] Biochemistry. 1973 Oct 9;12(21):4282-9 [4745672] J Mol Biol. 1974 Nov 15;89(4):737-55 [4475698] Biochemistry. 1981 Jan 6;20(1):33-7 [7470476] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Bcl-x and Stat5 Modulate Erythropoiesis through Distinct, Stagespecific Mechanisms T2 - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AN - 40107978; 3982025 JF - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AU - Zhu, Bing-Mei AU - Cui, Yongzhi AU - Heinrich, Achim AU - Klingmuller, Ursula AU - Liu, Jie AU - Robinson, Gertraud W AU - Hennighausen, Lothar Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 KW - Erythropoiesis KW - Bcl-x protein KW - Stat5 protein KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107978?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.atitle=Bcl-x+and+Stat5+Modulate+Erythropoiesis+through+Distinct%2C+Stagespecific+Mechanisms&rft.au=Zhu%2C+Bing-Mei%3BCui%2C+Yongzhi%3BHeinrich%2C+Achim%3BKlingmuller%2C+Ursula%3BLiu%2C+Jie%3BRobinson%2C+Gertraud+W%3BHennighausen%2C+Lothar&rft.aulast=Zhu&rft.aufirst=Bing-Mei&rft.date=2005-07-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.issn=&rft_id=info:doi/ L2 - http://www.ascb.org/meetings/summer05/ecb/index.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Integrin Signaling and Local Remodeling T2 - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AN - 40022553; 3982016 JF - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AU - Yamada, Ken Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 KW - Integrins KW - Signal transduction KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40022553?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.atitle=Integrin+Signaling+and+Local+Remodeling&rft.au=Yamada%2C+Ken&rft.aulast=Yamada&rft.aufirst=Ken&rft.date=2005-07-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.issn=&rft_id=info:doi/ L2 - http://www.ascb.org/meetings/summer05/ecb/index.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Spontaneous differentiation of mouse embryonic stem cells in vitro: Characterization by global gene expression profiles AN - 19485359; 8250490 AB - We characterized the temporal gene expression changes during four weeks of spontaneous differentiation of mouse ES cells in a monolayer culture in order to obtain better insight into the differentiation process. The overall gene expression pattern was changed dramatically during the first two weeks of spontaneous differentiation, but stabilized after the second week. Most of the genes regulated within the first two weeks of spontaneous differentiation were genes related to development including morphogenesis, cell differentiation, embryonic development, pattern specification, mesoderm development, post-embryonic development, and blastocyst development. While most of the ectoderm lineage related genes were down-regulated, genes related to the mesoderm or endoderm lineage were up-regulated through the first week and second week, respectively. This study revealed that the development of ectoderm lineage is a recessive process during the spontaneous differentiation of mouse ES cells in monolayer culture. Our time-course characterization might provide a useful time line for directed differentiation of mouse ES cells. JF - Biochemical and Biophysical Research Communications AU - Heo, J AU - Lee, J S AU - Chu, I S AU - Takahama, Y AU - Thorgeirsson, S S AD - Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA, snorri_s_thorgeirsson@nih.gov Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 1061 EP - 1069 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 332 IS - 4 SN - 0006-291X, 0006-291X KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Gene expression KW - Differentiation KW - Stem cells KW - blastocysts KW - Embryogenesis KW - Embryo cells KW - Ectoderm KW - Morphogenesis KW - Cell culture KW - Endoderm KW - Mesoderm KW - G 07730:Development & Cell Cycle KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19485359?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+and+Biophysical+Research+Communications&rft.atitle=Spontaneous+differentiation+of+mouse+embryonic+stem+cells+in+vitro%3A+Characterization+by+global+gene+expression+profiles&rft.au=Heo%2C+J%3BLee%2C+J+S%3BChu%2C+I+S%3BTakahama%2C+Y%3BThorgeirsson%2C+S+S&rft.aulast=Heo&rft.aufirst=J&rft.date=2005-07-15&rft.volume=332&rft.issue=4&rft.spage=1061&rft.isbn=&rft.btitle=&rft.title=Biochemical+and+Biophysical+Research+Communications&rft.issn=0006291X&rft_id=info:doi/10.1016%2Fj.bbrc.2005.04.173 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Gene expression; Differentiation; Embryogenesis; blastocysts; Stem cells; Embryo cells; Ectoderm; Morphogenesis; Endoderm; Cell culture; Mesoderm DO - http://dx.doi.org/10.1016/j.bbrc.2005.04.173 ER - TY - JOUR T1 - Fetal Alcohol Spectrum Disorders AN - 17667367; 6525020 AB - To complement the 2005 Annual Clinical Focus on medical genomics, AFP will be publishing a series of short reviews on genetic syndromes. This series was designed to increase awareness of these diseases so that family physicians can recognize and diagnose children with these disorders and understand the kind of care they might require in the future. The second review in this series discusses fetal alcohol syndrome and fetal alcohol spectrum disorders. JF - American Family Physician AU - Wattendorf, D J AU - Muenke, M AD - National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 279 EP - 282 VL - 72 IS - 2 SN - 0002-838X, 0002-838X KW - Toxicology Abstracts KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17667367?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Family+Physician&rft.atitle=Fetal+Alcohol+Spectrum+Disorders&rft.au=Wattendorf%2C+D+J%3BMuenke%2C+M&rft.aulast=Wattendorf&rft.aufirst=D&rft.date=2005-07-15&rft.volume=72&rft.issue=2&rft.spage=279&rft.isbn=&rft.btitle=&rft.title=American+Family+Physician&rft.issn=0002838X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Recent advances in biomarkers for cancer diagnosis and treatment AN - 17543392; 6412410 AB - Biomarker advances, benefiting from technological revolutions in the field of proteomics, genonomics, and epigenomics, offer hopes that one day cancer diagnosis and treatment will be guided by and individualized at the molecular levels. JF - Drug Discovery Today AU - Manne, U AU - Srivastava, R G AU - Srivastava, S Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 965 EP - 976 PB - Elsevier Ltd VL - 10 IS - 14 SN - 1359-6446, 1359-6446 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Drug discovery KW - Reviews KW - proteomics KW - biomarkers KW - Cancer KW - W 30965:Miscellaneous, Reviews KW - W3 33000:General topics and reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17543392?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+Discovery+Today&rft.atitle=Recent+advances+in+biomarkers+for+cancer+diagnosis+and+treatment&rft.au=Manne%2C+U%3BSrivastava%2C+R+G%3BSrivastava%2C+S&rft.aulast=Manne&rft.aufirst=U&rft.date=2005-07-15&rft.volume=10&rft.issue=14&rft.spage=965&rft.isbn=&rft.btitle=&rft.title=Drug+Discovery+Today&rft.issn=13596446&rft_id=info:doi/10.1016%2FS1359-6446%2805%2903487-2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; biomarkers; proteomics; Reviews; Drug discovery DO - http://dx.doi.org/10.1016/S1359-6446(05)03487-2 ER - TY - JOUR T1 - Effector Functions of Heparin-Binding Hemagglutinin-Specific CD8 super(+) T Lymphocytes in Latent Human Tuberculosis AN - 17502536; 6385800 AB - Background. Most individuals infected with Mycobacterium tuberculosis do not develop tuberculosis (TB) and can be regarded as being protected by an appropriate immune response to the infection. The characterization of the immune responses of individuals with latent TB may thus be helpful in the definition of correlates of protection and the development of new vaccine strategies. The highly protective antigen heparin-binding hemagglutinin (HBHA) induces strong interferon (IFN)- gamma responses during latent, but not active, TB. Because of the recently recognized importance of CD8 super(+) T lymphocytes in anti-TB immunity, we characterized the CD8 super(+) T lymphocyte responses to HBHA in subjects with latent TB. Results. HBHA-specific CD8 super(+) T lymphcoytes expressed memory cell markers and synthesized HBHA-specific IFN- gamma . They also restricted mycobacterial growth and expressed cytotoxicity by a granule-dependent mechanism. This activity was associated with the intracellular expression of HBHA-induced perforin. Surprisingly, the perforin-producing CD8 super(+) T lymphocytes were distinct from the IFN- gamma -producing CD8 super(+) T lymphocytes. Conclusion. During latent TB, the HBHA-specific CD8 super(+) T lymphocyte population expresses all 3 effector functions associated with CD8 super(+) T lymphocyte-mediated protective immune mechanisms, which supports the notion that HBHA may be protective in humans and suggests that markers of HBHA-specific CD8 super(+) T lymphcoyte responses may be useful in the monitoring of protection. JF - Journal of Infectious Diseases AU - Temmerman, ST AU - Place, S AU - Debrie, A-S AU - Locht, C AU - Mascart, F AD - Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 226 EP - 232 VL - 192 IS - 2 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology KW - Perforin KW - Hemagglutinins KW - protective antigen KW - Memory cells KW - Immunity KW - CD8 antigen KW - Cytotoxicity KW - ^g-Interferon KW - Lymphocytes T KW - Tuberculosis KW - Immune response KW - Vaccines KW - Mycobacterium tuberculosis KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17502536?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Effector+Functions+of+Heparin-Binding+Hemagglutinin-Specific+CD8+super%28%2B%29+T+Lymphocytes+in+Latent+Human+Tuberculosis&rft.au=Temmerman%2C+ST%3BPlace%2C+S%3BDebrie%2C+A-S%3BLocht%2C+C%3BMascart%2C+F&rft.aulast=Temmerman&rft.aufirst=ST&rft.date=2005-07-15&rft.volume=192&rft.issue=2&rft.spage=226&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; CD8 antigen; Lymphocytes T; ^g-Interferon; Tuberculosis; Perforin; Hemagglutinins; protective antigen; Memory cells; Vaccines; Immune response; Immunity; Cytotoxicity ER - TY - JOUR T1 - A Potentially Significant Interaction between Efavirenz and Phenytoin: A Case Report and Review of the Literature AN - 17501408; 6386026 AB - Although it has not been demonstrated yet, phenytoin is expected to reduce efavirenz exposure through coinduction of cytochrome P450 (CYP) 3A4 and CYP2B6. Conversely, efavirenz has been shown in vitro to inhibit the enzymes responsible for phenytoin metabolism, CYP2C9 and CYP2C19. We report a case in which a potential bidirectional drug interaction between phenytoin and efavirenz resulted in lower-than-expected efavirenz concentrations and elevated phenytoin levels. Therapeutic drug monitoring was used in this case to ensure adequate efavirenz exposure. JF - Clinical Infectious Diseases AU - Robertson, S M AU - Penzak AU - Lane, J AU - Pau, A K AU - Mican, J M AD - Clinical Pharmacokinetics Research Laboratory, Clinical Center Pharmacy Department, and Office of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - e15 EP - e18 VL - 41 IS - 2 SN - 1058-4838, 1058-4838 KW - Toxicology Abstracts KW - Drug interaction KW - Phenytoin KW - Efavirenz KW - Case reports KW - Reviews KW - Enzymes KW - Cytochrome P450 KW - Metabolism KW - X 24114:Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17501408?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Infectious+Diseases&rft.atitle=A+Potentially+Significant+Interaction+between+Efavirenz+and+Phenytoin%3A+A+Case+Report+and+Review+of+the+Literature&rft.au=Robertson%2C+S+M%3BPenzak%3BLane%2C+J%3BPau%2C+A+K%3BMican%2C+J+M&rft.aulast=Robertson&rft.aufirst=S&rft.date=2005-07-15&rft.volume=41&rft.issue=2&rft.spage=e15&rft.isbn=&rft.btitle=&rft.title=Clinical+Infectious+Diseases&rft.issn=10584838&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Efavirenz; Phenytoin; Case reports; Cytochrome P450; Metabolism; Reviews; Drug interaction; Enzymes ER - TY - JOUR T1 - Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. AN - 68029576; 16015321 AB - Transposons have provided important genetic tools for functional genomic screens in lower eukaryotes but have proven less useful in higher eukaryotes because of their low transposition frequency. Here we show that Sleeping Beauty (SB), a member of the Tc1/mariner class of transposons, can be mobilized in mouse somatic cells at frequencies high enough to induce embryonic death and cancer in wild-type mice. Tumours are aggressive, with some animals developing two or even three different types of cancer within a few months of birth. The tumours result from SB insertional mutagenesis of cancer genes, thus facilitating the identification of genes and pathways that induce disease. SB transposition can easily be controlled to mutagenize any target tissue and can therefore, in principle, be used to induce many of the cancers affecting humans, including those for which little is known about the aetiology. The uses of SB are also not restricted to the mouse and could potentially be used for forward genetic screens in any higher eukaryote in which transgenesis is possible. JF - Nature AU - Dupuy, Adam J AU - Akagi, Keiko AU - Largaespada, David A AU - Copeland, Neal G AU - Jenkins, Nancy A AD - National Cancer Institute, Center for Cancer Research, Frederick, Maryland 21702, USA. Y1 - 2005/07/14/ PY - 2005 DA - 2005 Jul 14 SP - 221 EP - 226 VL - 436 IS - 7048 KW - DNA Transposable Elements KW - 0 KW - Notch1 protein, mouse KW - Receptor, Notch1 KW - Receptors, Cell Surface KW - Transcription Factors KW - Transposases KW - EC 2.7.7.- KW - Index Medicus KW - Animals KW - Transposases -- metabolism KW - Transcription Factors -- metabolism KW - Mice KW - Embryo, Mammalian -- metabolism KW - Mice, Transgenic KW - Transcription Factors -- genetics KW - Embryo Loss -- genetics KW - Neoplasms -- genetics KW - Receptors, Cell Surface -- metabolism KW - Phenotype KW - Alleles KW - Oncogenes -- genetics KW - Transposases -- genetics KW - Receptors, Cell Surface -- genetics KW - Cell Transformation, Neoplastic -- genetics KW - Mutagenesis, Insertional -- methods KW - DNA Transposable Elements -- genetics KW - Mammals -- genetics KW - Mutagenesis, Insertional -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68029576?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Mammalian+mutagenesis+using+a+highly+mobile+somatic+Sleeping+Beauty+transposon+system.&rft.au=Dupuy%2C+Adam+J%3BAkagi%2C+Keiko%3BLargaespada%2C+David+A%3BCopeland%2C+Neal+G%3BJenkins%2C+Nancy+A&rft.aulast=Dupuy&rft.aufirst=Adam&rft.date=2005-07-14&rft.volume=436&rft.issue=7048&rft.spage=221&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-07-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Nature. 2005 Jul 14;436(7048):184-6 [16015313] Cell. 2005 Aug 12;122(3):322-5 [16096053] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Use of Facs-Assisted Microdissection to isolate Germ-Layer Specific Genes T2 - 4th International European Zebrafish Meeting on Development and Genetics AN - 40107854; 3980735 JF - 4th International European Zebrafish Meeting on Development and Genetics AU - Boorech, J L AU - Kirby, M R AU - Elkahloun, A G AU - Feldman, B Y1 - 2005/07/13/ PY - 2005 DA - 2005 Jul 13 KW - Freshwater fish KW - Genetics KW - Development KW - Danio rerio KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107854?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.atitle=Use+of+Facs-Assisted+Microdissection+to+isolate+Germ-Layer+Specific+Genes&rft.au=Boorech%2C+J+L%3BKirby%2C+M+R%3BElkahloun%2C+A+G%3BFeldman%2C+B&rft.aulast=Boorech&rft.aufirst=J&rft.date=2005-07-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.issn=&rft_id=info:doi/ L2 - https://www.zebrafish-dresden.com/pub/b/frame.asp?f=2&mid=12&sid=1&m=12 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Comparison of the Mutation Detection Frequency in Reverse Genetic Screens by Tillingand Re-Sequencing Technologies T2 - 4th International European Zebrafish Meeting on Development and Genetics AN - 40105460; 3980784 JF - 4th International European Zebrafish Meeting on Development and Genetics AU - Sood, R AU - Jones, M P AU - Zhang, J AU - Mullikin, J AU - Wu, D Y AU - Wang, D M AU - Yu, J. AU - Liu, P P Y1 - 2005/07/13/ PY - 2005 DA - 2005 Jul 13 KW - Mutation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40105460?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.atitle=Comparison+of+the+Mutation+Detection+Frequency+in+Reverse+Genetic+Screens+by+Tillingand+Re-Sequencing+Technologies&rft.au=Sood%2C+R%3BJones%2C+M+P%3BZhang%2C+J%3BMullikin%2C+J%3BWu%2C+D+Y%3BWang%2C+D+M%3BYu%2C+J.%3BLiu%2C+P+P&rft.aulast=Sood&rft.aufirst=R&rft.date=2005-07-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.issn=&rft_id=info:doi/ L2 - https://www.zebrafish-dresden.com/pub/b/frame.asp?f=2&mid=12&sid=1&m=12 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Mutation of the adenylylated tyrosine of glutamine synthetase alters its catalytic properties. AN - 68000907; 15996098 AB - Glutamine synthetase is central to nitrogen metabolism in the Gram-negative bacteria. The amount of glutamine synthetase in the cell and its catalytic activity are tightly regulated by multiple, sophisticated mechanisms. Reversible covalent modification of Tyr-397 is central to the regulation of glutamine synthetase activity, via esterification of the hydroxyl group to AMP in a process termed adenylylation. As expected, site-specific mutation of this surface-exposed Tyr-397 to Phe, Ala, or Ser was found to prevent adenylylation. Unexpectedly, these mutations had major effects on the catalytic characteristics of glutamine synthetase. The specific activities of each mutant were approximately doubled, the pH-activity profiles changed, and divalent-cation specificity was altered. Overall, Tyr397Phe behaved as if it were unadenylylated, while both Tyr397Ala and Tyr397Ser behaved as if they were adenylylated. Thus, subtle modifications in the environment of residue 397 are sufficient to induce changes previously thought to require adenylylation. JF - Biochemistry AU - Luo, Shen AU - Kim, Geumsoo AU - Levine, Rodney L AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/07/12/ PY - 2005 DA - 2005 Jul 12 SP - 9441 EP - 9446 VL - 44 IS - 27 SN - 0006-2960, 0006-2960 KW - Cations, Divalent KW - 0 KW - Escherichia coli Proteins KW - Hydroxides KW - Glutamine KW - 0RH81L854J KW - Adenosine Monophosphate KW - 415SHH325A KW - Tyrosine KW - 42HK56048U KW - Manganese KW - 42Z2K6ZL8P KW - Serine KW - 452VLY9402 KW - Phenylalanine KW - 47E5O17Y3R KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - hydroxide ion KW - 9159UV381P KW - gamma-Glutamyltransferase KW - EC 2.3.2.2 KW - Glutamate-Ammonia Ligase KW - EC 6.3.1.2 KW - Magnesium KW - I38ZP9992A KW - Adenine KW - JAC85A2161 KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Cations, Divalent -- metabolism KW - Feedback, Physiological -- genetics KW - Hydrogen-Ion Concentration KW - gamma-Glutamyltransferase -- metabolism KW - Glutamine -- biosynthesis KW - Serine -- genetics KW - Enzyme Activation -- genetics KW - Esterification KW - Manganese -- chemistry KW - Alanine -- genetics KW - Glutamine -- chemistry KW - Phenylalanine -- genetics KW - Hydroxides -- metabolism KW - Magnesium -- chemistry KW - Adenosine Diphosphate -- metabolism KW - Catalysis KW - Mutagenesis, Site-Directed KW - Glutamate-Ammonia Ligase -- genetics KW - Glutamate-Ammonia Ligase -- antagonists & inhibitors KW - Glutamate-Ammonia Ligase -- chemistry KW - Escherichia coli Proteins -- metabolism KW - Glutamate-Ammonia Ligase -- metabolism KW - Adenine -- metabolism KW - Adenosine Monophosphate -- metabolism KW - Tyrosine -- genetics KW - Tyrosine -- metabolism KW - Escherichia coli Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68000907?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Mutation+of+the+adenylylated+tyrosine+of+glutamine+synthetase+alters+its+catalytic+properties.&rft.au=Luo%2C+Shen%3BKim%2C+Geumsoo%3BLevine%2C+Rodney+L&rft.aulast=Luo&rft.aufirst=Shen&rft.date=2005-07-12&rft.volume=44&rft.issue=27&rft.spage=9441&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nonmelanoma skin cancer in relation to ionizing radiation exposure among U.S. radiologic technologists. AN - 67831418; 15704092 AB - Ionizing radiation (IR) is an established cause of nonmelanoma skin cancer, but there is uncertainty about the risk associated with chronic occupational exposure to IR and how it is influenced by ultraviolet radiation (UVR) exposure. We studied 1,355 incident cases with basal cell carcinoma (BCC) and 270 with squamous cell carcinoma (SCC) of the skin in a cohort of 65,304 U.S. white radiologic technologists who responded to the baseline questionnaire survey in 1983-1989 and the follow-up survey in 1994-1998. Cox's proportional-hazards model was used to estimate relative risks of BCC and SCC associated with surrogate measures of occupational exposure to IR and residential UVR exposure during childhood and adulthood, adjusted for potential confounders including pigmentation characteristics. Relative risks of BCC, but not of SCC, were elevated among technologists who first worked during the 1950s (RR = 1.42; 95% CI = 1.12-1.80), 1940s (RR = 2.04; 95% CI = 1.44-2.88) and before 1940 (RR = 2.16; 95% CI = 1.14-4.09), when IR exposures were high, compared to those who first worked after 1960 (p for trend < 0.01). The effect of year first worked on BCC risk was not modified by UVR exposure, but was significantly stronger among individuals with lighter compared to darker eye and hair color (p = 0.013 and 0.027, respectively). This study provides some evidence that chronic occupational exposure to IR at low to moderate levels can increase the risk of BCC, and that this risk may be modified by pigmentation characteristics. (c) 2005 Wiley-Liss, Inc. JF - International journal of cancer AU - Yoshinaga, Shinji AU - Hauptmann, Michael AU - Sigurdson, Alice J AU - Doody, Michele Morin AU - Freedman, D Michal AU - Alexander, Bruce H AU - Linet, Martha S AU - Ron, Elaine AU - Mabuchi, Kiyohiko AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. yosinaga@nirs.go.jp Y1 - 2005/07/10/ PY - 2005 DA - 2005 Jul 10 SP - 828 EP - 834 VL - 115 IS - 5 SN - 0020-7136, 0020-7136 KW - Index Medicus KW - Allied Health Personnel KW - Risk Factors KW - Humans KW - Cohort Studies KW - Adult KW - Aged KW - Middle Aged KW - Skin Pigmentation KW - Male KW - Female KW - Radiation, Ionizing KW - Occupational Exposure KW - Carcinoma, Squamous Cell -- etiology KW - Carcinoma, Squamous Cell -- epidemiology KW - Carcinoma, Basal Cell -- etiology KW - Skin Neoplasms -- etiology KW - Radiology -- manpower KW - Skin Neoplasms -- epidemiology KW - Radiation Injuries -- epidemiology KW - Carcinoma, Basal Cell -- epidemiology KW - Radiation Injuries -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67831418?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+cancer&rft.atitle=Nonmelanoma+skin+cancer+in+relation+to+ionizing+radiation+exposure+among+U.S.+radiologic+technologists.&rft.au=Yoshinaga%2C+Shinji%3BHauptmann%2C+Michael%3BSigurdson%2C+Alice+J%3BDoody%2C+Michele+Morin%3BFreedman%2C+D+Michal%3BAlexander%2C+Bruce+H%3BLinet%2C+Martha+S%3BRon%2C+Elaine%3BMabuchi%2C+Kiyohiko&rft.aulast=Yoshinaga&rft.aufirst=Shinji&rft.date=2005-07-10&rft.volume=115&rft.issue=5&rft.spage=828&rft.isbn=&rft.btitle=&rft.title=International+journal+of+cancer&rft.issn=00207136&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-29 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protein kinase C phosphorylation of the metabotropic glutamate receptor mGluR5 on Serine 839 regulates Ca2+ oscillations. AN - 67997857; 15894802 AB - The activation of Group 1 metabotropic glutamate receptors, mGluR5 and mGluR1alpha, triggers intracellular calcium release; however, mGluR5 activation is unique in that it elicits Ca2+ oscillations. A short region of the mGluR5 C terminus is the critical determinant and differs from the analogous region of mGluR1alpha by a single amino acid residue, Thr-840, which is an aspartic acid (Asp-854) in mGluR1alpha. Previous studies show that mGluR5-elicited Ca2+ oscillations require protein kinase C (PKC)-dependent phosphorylation and identify Thr-840 as the phosphorylation site. However, direct phosphorylation of mGluR5 has not been studied in detail. We have used biochemical analyses to directly investigate the phosphorylation of the mGluR5 C terminus. We showed that Ser-839 on mGluR5 is directly phosphorylated by PKC, whereas Thr-840 plays a permissive role. Although Ser-839 is conserved in mGluR1alpha (Ser-853), it is not phosphorylated, as the adjacent residue (Asp-854) is not permissive; however, mutagenesis of Asp-854 to a permissive alanine residue allows phosphorylation of Ser-853 on mGluR1alpha. We investigated the physiological consequences of mGluR5 Ser-839 phosphorylation using Ca2+ imaging. Mutations that eliminate Ser-839 phosphorylation prevent the characteristic mGluR5-dependent Ca2+ oscillations. However, mutation of Thr-840 to alanine, which prevents potential Thr-840 phosphorylation but is still permissive for Ser-839 phosphorylation, has no effect on Ca2+ oscillations. Thus, we showed that it is phosphorylation of Ser-839, not Thr-840, that is absolutely required for the unique Ca2+ oscillations produced by mGluR5 activation. The Thr-840 residue is important only in that it is permissive for the PKC-dependent phosphorylation of Ser-839. JF - The Journal of biological chemistry AU - Kim, Chul Hoon AU - Braud, Stephanie AU - Isaac, John T R AU - Roche, Katherine W AD - NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07/08/ PY - 2005 DA - 2005 Jul 08 SP - 25409 EP - 25415 VL - 280 IS - 27 SN - 0021-9258, 0021-9258 KW - Antibodies KW - 0 KW - GRM5 protein, human KW - Receptor, Metabotropic Glutamate 5 KW - Receptors, Metabotropic Glutamate KW - Serine KW - 452VLY9402 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Animals KW - Phosphorylation KW - HeLa Cells KW - Humans KW - Molecular Sequence Data KW - Rabbits KW - Amino Acid Sequence KW - Serine -- metabolism KW - Serine -- immunology KW - Protein Kinase C -- metabolism KW - Calcium Signaling -- physiology KW - Receptors, Metabotropic Glutamate -- genetics KW - Receptors, Metabotropic Glutamate -- metabolism KW - Receptors, Metabotropic Glutamate -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67997857?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C+phosphorylation+of+the+metabotropic+glutamate+receptor+mGluR5+on+Serine+839+regulates+Ca2%2B+oscillations.&rft.au=Kim%2C+Chul+Hoon%3BBraud%2C+Stephanie%3BIsaac%2C+John+T+R%3BRoche%2C+Katherine+W&rft.aulast=Kim&rft.aufirst=Chul&rft.date=2005-07-08&rft.volume=280&rft.issue=27&rft.spage=25409&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Thionylation of PKC by Reactive Sulfur Species: Disulfide S-Monoxide and Disulfide S-Dioxide T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40041156; 3969310 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Huang, F L AU - Huang, K.-P. Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Protein kinase C KW - Sulfur KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041156?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Thionylation+of+PKC+by+Reactive+Sulfur+Species%3A+Disulfide+S-Monoxide+and+Disulfide+S-Dioxide&rft.au=Huang%2C+F+L%3BHuang%2C+K.-P.&rft.aulast=Huang&rft.aufirst=F&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Drosophila Myosin V: Solution Kinetics and Motile Properties T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40013825; 3969057 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Toth, J AU - Kovacs, M AU - Wang, F AU - Nyitray, L AU - Sellers, J R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Kinetics KW - Myosin KW - Drosophila KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40013825?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Drosophila+Myosin+V%3A+Solution+Kinetics+and+Motile+Properties&rft.au=Toth%2C+J%3BKovacs%2C+M%3BWang%2C+F%3BNyitray%2C+L%3BSellers%2C+J+R&rft.aulast=Toth&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - SC35 Promotes Prolonged Stress- Induced 3Alternative Splicing of Acetylcholinesterase T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40013449; 3968169 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Meshorer, E AU - Toiber, D AU - Bryk, B AU - Dori, A AU - Soreq, H Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Acetylcholinesterase KW - Splicing KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40013449?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=SC35+Promotes+Prolonged+Stress-+Induced+3Alternative+Splicing+of+Acetylcholinesterase&rft.au=Meshorer%2C+E%3BToiber%2C+D%3BBryk%2C+B%3BDori%2C+A%3BSoreq%2C+H&rft.aulast=Meshorer&rft.aufirst=E&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Phosphorylation and O-Glycosylation Sites Identification in Peptides by Ba-Hydroxide Catalyzed b-Elimination/Propanethiol Addition and Mass Spectrometric Analyses T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40008412; 3969653 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Chen, H.-C. AU - Wang, G AU - Backlund, P S AU - Boykins, R A Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Phosphorylation KW - Peptides KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40008412?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Phosphorylation+and+O-Glycosylation+Sites+Identification+in+Peptides+by+Ba-Hydroxide+Catalyzed+b-Elimination%2FPropanethiol+Addition+and+Mass+Spectrometric+Analyses&rft.au=Chen%2C+H.-C.%3BWang%2C+G%3BBacklund%2C+P+S%3BBoykins%2C+R+A&rft.aulast=Chen&rft.aufirst=H.-C.&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - New ABC Transporters Associated with Multidrug Resistance in Cancer T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40003226; 3968650 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Gottesman, M M AU - Paterson, J K AU - Chen, K G AU - Annereau, J P AU - Szakacs, G Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - ABC transporter KW - Multidrug resistance KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40003226?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=New+ABC+Transporters+Associated+with+Multidrug+Resistance+in+Cancer&rft.au=Gottesman%2C+M+M%3BPaterson%2C+J+K%3BChen%2C+K+G%3BAnnereau%2C+J+P%3BSzakacs%2C+G&rft.aulast=Gottesman&rft.aufirst=M&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - ABCB6 Overexpression is Associated with Resistance to Arsenite, Cisplatin, and Antimony T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40002442; 3969912 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Paterson, J K AU - Tachiwada, T AU - Szakacs, G AU - Akiyama, S.-i. AU - Gottesman, M M Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Cisplatin KW - Antimony KW - Arsenite KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40002442?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=ABCB6+Overexpression+is+Associated+with+Resistance+to+Arsenite%2C+Cisplatin%2C+and+Antimony&rft.au=Paterson%2C+J+K%3BTachiwada%2C+T%3BSzakacs%2C+G%3BAkiyama%2C+S.-i.%3BGottesman%2C+M+M&rft.aulast=Paterson&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Regulation and Mechanics of Myosin V T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39998435; 3969047 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Sellers, J R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Myosin KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39998435?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Regulation+and+Mechanics+of+Myosin+V&rft.au=Sellers%2C+J+R&rft.aulast=Sellers&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Structure and Expression of p53 Gene and Altered Phosphorylation of p53 Protein in Human Vestibular Schwannomas T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39998041; 3968120 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Prabhu, P. Antony Herold AU - Mathivanan, J AU - Rohini, K AU - Thomas, R AU - Chandramouli, B AU - Gope, R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Schwann cells KW - Phosphorylation KW - Vestibular system KW - p53 protein KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39998041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Structure+and+Expression+of+p53+Gene+and+Altered+Phosphorylation+of+p53+Protein+in+Human+Vestibular+Schwannomas&rft.au=Prabhu%2C+P.+Antony+Herold%3BMathivanan%2C+J%3BRohini%2C+K%3BThomas%2C+R%3BChandramouli%2C+B%3BGope%2C+R&rft.aulast=Prabhu&rft.aufirst=P.+Antony&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Arrested Apoptosis in Lens Fiber Cells: A Possible Role of a-Crystallin T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39996834; 3968433 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Morozov, V AU - Wawrousek, E F Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - crystallin KW - Apoptosis KW - Eye lens KW - Fibers KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39996834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Arrested+Apoptosis+in+Lens+Fiber+Cells%3A+A+Possible+Role+of+a-Crystallin&rft.au=Morozov%2C+V%3BWawrousek%2C+E+F&rft.aulast=Morozov&rft.aufirst=V&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Fanconi anemia: adult head and neck cancer and hematopoietic mosaicism. AN - 85387542; pmid-16027289 JF - Archives of otolaryngology--head & neck surgery AU - Alter, Blanche P AU - Joenje, Hans AU - Oostra, Anneke B AU - Pals, Gerard AD - Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Bethesda, MD, USA. alterb@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 635 EP - 639 VL - 131 IS - 7 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - Adult KW - *Carcinoma, Squamous Cell: etiology KW - Carcinoma, Squamous Cell: genetics KW - Chromosome Breakage KW - *Fanconi Anemia: complications KW - Fanconi Anemia: genetics KW - Female KW - *Head and Neck Neoplasms: etiology KW - Head and Neck Neoplasms: genetics KW - Humans KW - Male KW - Middle Aged KW - *Mosaicism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85387542?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Fanconi+anemia%3A+adult+head+and+neck+cancer+and+hematopoietic+mosaicism.&rft.au=Alter%2C+Blanche+P%3BJoenje%2C+Hans%3BOostra%2C+Anneke+B%3BPals%2C+Gerard&rft.aulast=Alter&rft.aufirst=Blanche&rft.date=2005-07-01&rft.volume=131&rft.issue=7&rft.spage=635&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - SuppNotes - Comment In: Arch Otolaryngol Head Neck Surg. 2005 Jul;131(7):640-1[16027290] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Head and neck squamous cell carcinoma in patients with Fanconi anemia. AN - 85383731; pmid-16027290 JF - Archives of otolaryngology--head & neck surgery AU - Van Waes, Carter AD - Head and Neck Surgery Branch, National Institute on Deafness and Other Communication Disorders, Bethesda, MD 20892-1462, USA. vanwaesc@nidcd.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 640 EP - 641 VL - 131 IS - 7 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - *Carcinoma, Squamous Cell: etiology KW - Carcinoma, Squamous Cell: genetics KW - *Fanconi Anemia: complications KW - Fanconi Anemia: genetics KW - *Head and Neck Neoplasms: etiology KW - Head and Neck Neoplasms: genetics KW - Humans UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85383731?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Head+and+neck+squamous+cell+carcinoma+in+patients+with+Fanconi+anemia.&rft.au=Van+Waes%2C+Carter&rft.aulast=Van+Waes&rft.aufirst=Carter&rft.date=2005-07-01&rft.volume=131&rft.issue=7&rft.spage=640&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - SuppNotes - Comment On: Arch Otolaryngol Head Neck Surg. 2005 Jul;131(7):635-9[16027289] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Mutation of the Parkin gene in a Persian family: clinical progression over a 40-year period. AN - 85381972; pmid-15852366 AB - We report on an Israeli family originating from Iran in which 4 of 7 brothers born from a consanguineous marriage had juvenile Parkinsonism. Linkage analysis of markers covering the autosomal recessive juvenile Parkinsonism (AR-JP, PARK2, Parkin gene, OMIM #602544) gene resulted in a maximal logarithm of odds score of 2.18. A homozygous deletion that expanded from exon 4 to exon 6 was identified in all the patients. Significant clinical heterogeneity was present between siblings.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Clarimon, Jordi AU - Johnson, Janel AU - Djaldetti, Ruth AU - Hernandez, Dena AU - Hattori, Nobutaka AU - Sroka, Hava AU - Barhom, Yael AU - Singleton, Andrew AD - Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, USA. clarimon@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 887 EP - 890 VL - 20 IS - 7 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Aged KW - DNA Mutational Analysis: methods KW - Exons KW - Female KW - Humans KW - Iran KW - Male KW - *Mutation KW - Odds Ratio KW - *Parkinsonian Disorders: genetics KW - Pedigree KW - RNA, Messenger: biosynthesis KW - Reverse Transcriptase Polymerase Chain Reaction: methods KW - *Siblings KW - *Ubiquitin-Protein Ligases: genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85381972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Mutation+of+the+Parkin+gene+in+a+Persian+family%3A+clinical+progression+over+a+40-year+period.&rft.au=Clarimon%2C+Jordi%3BJohnson%2C+Janel%3BDjaldetti%2C+Ruth%3BHernandez%2C+Dena%3BHattori%2C+Nobutaka%3BSroka%2C+Hava%3BBarhom%2C+Yael%3BSingleton%2C+Andrew&rft.aulast=Clarimon&rft.aufirst=Jordi&rft.date=2005-07-01&rft.volume=20&rft.issue=7&rft.spage=887&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Polymeric Scaffolds for Cartilage Tissue Engineering AN - 754562450; 13386477 AB - Polymeric scaffolds are three-dimensional, porous structures that may be used as a vehicle to deliver cells or therapeutic factors to repair tissue defects. Both biodegradable and non-biodegradable polymers have been developed for this purpose. In this review, we survey the polymers that have been investigated for cartilage tissue engineering and discuss the critical parameters for successful applications in the future. JF - Macromolecular Symposia AU - Li, Wan-Ju AU - Tuan, Rocky S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA,, Tuanr@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 65 EP - 76 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 227 IS - 1 SN - 1022-1360, 1022-1360 KW - Biotechnology and Bioengineering Abstracts; Calcium & Calcified Tissue Abstracts KW - Macromolecules KW - Cartilage KW - Tissue engineering KW - Biodegradability KW - scaffolds KW - T 2030:Cartilage and Cartilage Diseases KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/754562450?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Macromolecular+Symposia&rft.atitle=Polymeric+Scaffolds+for+Cartilage+Tissue+Engineering&rft.au=Li%2C+Wan-Ju%3BTuan%2C+Rocky+S&rft.aulast=Li&rft.aufirst=Wan-Ju&rft.date=2005-07-01&rft.volume=227&rft.issue=1&rft.spage=65&rft.isbn=&rft.btitle=&rft.title=Macromolecular+Symposia&rft.issn=10221360&rft_id=info:doi/10.1002%2Fmasy.200550906 L2 - http://www3.interscience.wiley.com/journal/110576993/abstract LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-08-01 N1 - Last updated - 2015-03-19 N1 - SubjectsTermNotLitGenreText - Macromolecules; Cartilage; Tissue engineering; Biodegradability; scaffolds DO - http://dx.doi.org/10.1002/masy.200550906 ER - TY - JOUR T1 - Cancer biology and hormesis: comments on Calabrese (2005). AN - 69072980; 16422393 AB - Large numbers of chemicals of a variety of types exhibit apparent hormetic effects on cultured human cancer cells, causing stimulation of cell growth or related changes at low doses, followed by inhibition at higher doses. Many of the studies listed are not fully convincing, due to lack of appropriate controls or sufficient number of doses. However, the proposed hormetic response seems firmly established in a subset of these experiments. Significance with regard to in vivo cancer growth has not been pursued and must be a priority for the future. For several examples where in vivo titers are known, such as, resveratrol, suramin, and tamoxifen, comparison of the dose and concentration ranges confirms that hormesis could be an issue in vivo. Further investigations are warranted, especially for therapeutic drugs, phytochemicals, and environmental toxicants. JF - Critical reviews in toxicology AU - Anderson, Lucy M AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, Ft Detrick, Maryland 21702, USA. Andersol@ncifcrf.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 583 EP - 586 VL - 35 IS - 6 SN - 1040-8444, 1040-8444 KW - Antineoplastic Agents KW - 0 KW - Carcinogens KW - Stilbenes KW - Tamoxifen KW - 094ZI81Y45 KW - Suramin KW - 6032D45BEM KW - resveratrol KW - Q369O8926L KW - Index Medicus KW - Tamoxifen -- pharmacology KW - No-Observed-Adverse-Effect Level KW - Tumor Cells, Cultured KW - Stilbenes -- pharmacology KW - Dose-Response Relationship, Drug KW - Humans KW - Cell Line, Tumor KW - Suramin -- pharmacology KW - Risk Assessment KW - Adaptation, Physiological -- drug effects KW - Adaptation, Physiological -- physiology KW - Cell Division -- drug effects KW - Carcinogens -- toxicity KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/69072980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Critical+reviews+in+toxicology&rft.atitle=Cancer+biology+and+hormesis%3A+comments+on+Calabrese+%282005%29.&rft.au=Anderson%2C+Lucy+M&rft.aulast=Anderson&rft.aufirst=Lucy&rft.date=2005-07-01&rft.volume=35&rft.issue=6&rft.spage=583&rft.isbn=&rft.btitle=&rft.title=Critical+reviews+in+toxicology&rft.issn=10408444&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-02 N1 - Date created - 2006-01-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment On: Crit Rev Toxicol. 2005 Jul;35(6):463-582 [16422392] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Medical management of HIV-hepatitis C virus coinfection in injection drug users. AN - 68752312; 16265605 AB - Several million people inject drugs of abuse and, as a result, are coinfected with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). The treatment of this coinfected drug-abusing population is fraught with many problems such that clinicians and other health care providers have to determine whether patients should be treated first for drug addiction, for HIV/AIDS, or for HCV infection or simultaneously treated. These proceedings present the incidence and prevalence of coinfections with HIV and HCV in high-risk populations and discuss the underlying pathophysiology of coinfections and the problems and strategies of managing the treatment of coinfections among people who also inject illicit drugs. In addition, the expert panel recommended further research to determine the best possible treatment regimens applicable to injection drug users coinfected with HIV and HCV. JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Khalsa, Jag H AU - Kresina, Tom AU - Sherman, Ken AU - Vocci, Francis AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, National Institute on Drug Abuse, National Institutes of Health, Bethesda, Maryland 20892-9593, USA. jk98p@nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - S1 EP - S6 VL - 41 Suppl 1 KW - Index Medicus KW - Humans KW - Incidence KW - Prevalence KW - Hepatitis C -- drug therapy KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - Disease Management KW - HIV Infections -- drug therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- epidemiology KW - Hepatitis C -- epidemiology KW - Substance Abuse, Intravenous -- complications KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68752312?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Medical+management+of+HIV-hepatitis+C+virus+coinfection+in+injection+drug+users.&rft.au=Khalsa%2C+Jag+H%3BKresina%2C+Tom%3BSherman%2C+Ken%3BVocci%2C+Francis&rft.aulast=Khalsa&rft.aufirst=Jag&rft.date=2005-07-01&rft.volume=41+Suppl+1&rft.issue=&rft.spage=S1&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Integrating care for hepatitis C virus (HCV) and primary care for HIV for injection drug users coinfected with HIV and HCV. AN - 68751300; 16265621 AB - Injection drug use accounts for most of the incident infections with hepatitis C virus (HCV) and for at least one-third of new human immunodeficiency virus (HIV) infections. Coinfection with HCV and HIV presents complex and challenging medical conditions. Ensuring access to and maintaining care for HIV and HCV for drug users presents special challenges to the health care team that require a nonjudgmental attitude, experience, and patience. Care for HCV infection, however, can be used as an instrument to engage drug-using persons in ongoing primary care relationships. Common elements to both care for HCV infection and primary care for HIV infection are testing for and counseling about HCV and HIV, substance abuse and mental health services, social support, and subspecialty referral. These elements, in particular treatment for substance abuse, can be focal points for model care systems that provide integrative care for both HCV and HIV infections. JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Kresina, Thomas F AU - Bruce, R Douglas AU - Cargill, Victoria A AU - Cheever, Laura W AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, National Institute on Drug Abuse, Bethesda, MD 20892, USA. tk13v@nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - S83 EP - S88 VL - 41 Suppl 1 KW - Index Medicus KW - Substance Abuse Treatment Centers -- organization & administration KW - Humans KW - Professional-Patient Relations KW - Primary Health Care -- organization & administration KW - Male KW - Female KW - Hepatitis C -- therapy KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - HIV Infections -- therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- complications KW - Delivery of Health Care, Integrated UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68751300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Integrating+care+for+hepatitis+C+virus+%28HCV%29+and+primary+care+for+HIV+for+injection+drug+users+coinfected+with+HIV+and+HCV.&rft.au=Kresina%2C+Thomas+F%3BBruce%2C+R+Douglas%3BCargill%2C+Victoria+A%3BCheever%2C+Laura+W&rft.aulast=Kresina&rft.aufirst=Thomas&rft.date=2005-07-01&rft.volume=41+Suppl+1&rft.issue=&rft.spage=S83&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - No correlation between radiosensitivity or double-strand break repair capacity of normal fibroblasts and acute normal tissue reaction after radiotherapy of breast cancer patients. AN - 68750475; 16263653 AB - The aim was to study the relationship between cellular radiosensitivity or double-strand break (dsb) repair capacity of skin fibroblasts and the extent of acute reaction after radiotherapy for breast cancer. The study was performed with 25 breast cancer patients submitted to the radiotherapy unit of the Egyptian National Cancer Institute after conserving surgery. Dermal fibroblasts, established from skin biopsies, were used to determine the cellular radiosensitivity via colony assay and the capacity of dsb repair by constant-field gel electrophoresis. Acute reactions were scored using the Radiation Therapy Oncology Group (RTOG) classification. The spectrum of acute reactions varied from grade 1 to 4, whereby most patients developed a grade 1 reaction after total doses ranging between 46 and 70 Gy. Skin fibroblasts showed a pronounced variation in both cellular radiosensitivity expressed as the mean inactivation dose (Dbar) (coefficient of variation, CV=25%) as well as in the number of residual dsb (CV=33%) with no significant correlation between these two endpoints (r2=0.20, p=0.14). Both parameters did not correlate with the extent of acute reaction of the respective patient. The data obtained indicate that the sensitivity of fibroblasts measured either by colony assay or by dsb repair capacity is not a major parameter determining the extent of acute reaction after radiotherapy of breast cancer patients. JF - International journal of radiation biology AU - El-Awady, R A AU - Mahmoud, M AU - Saleh, E M AU - El-Baky, H Abd AU - Lotayef, M AU - Dahm-Daphi, J AU - Dikomey, E AD - Tumour Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt, and Department of Radiotherapy and Radiation Oncology, University-Hospital Hamburg-Eppendorf, Hamburg, Germany. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 501 EP - 508 VL - 81 IS - 7 SN - 0955-3002, 0955-3002 KW - Index Medicus KW - Space life sciences KW - Severity of Illness Index KW - Skin -- radiation effects KW - Humans KW - Adult KW - Skin -- cytology KW - Radiation Tolerance KW - Middle Aged KW - Biopsy KW - Female KW - Fibroblasts KW - DNA Repair KW - DNA Damage KW - Radiation Injuries -- classification KW - Breast Neoplasms -- radiotherapy KW - Radiation Injuries -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68750475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+biology&rft.atitle=No+correlation+between+radiosensitivity+or+double-strand+break+repair+capacity+of+normal+fibroblasts+and+acute+normal+tissue+reaction+after+radiotherapy+of+breast+cancer+patients.&rft.au=El-Awady%2C+R+A%3BMahmoud%2C+M%3BSaleh%2C+E+M%3BEl-Baky%2C+H+Abd%3BLotayef%2C+M%3BDahm-Daphi%2C+J%3BDikomey%2C+E&rft.aulast=El-Awady&rft.aufirst=R&rft.date=2005-07-01&rft.volume=81&rft.issue=7&rft.spage=501&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+biology&rft.issn=09553002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-13 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Anticarcinogenic effects of an aqueous infusion of cloves on skin carcinogenesis. AN - 68708457; 16235990 AB - Spices and flavouring agents are now receiving increasing attention as many of them have been shown to have anticarcinogenic properties. Cloves, sun-dried unopened flower buds from the plant Syzygium aromaticum L, are commonly used as a spice and food flavour. The present study was designed to investigate the chemopreventive action of aqueous infusion of cloves on 9,10-dimethyl benz(a)anthracene (DMBA) and croton oil induced skin carcinogenesis in Swiss mice. The results indicate protection against skin papilloma formation in a dose dependent manner. It has been shown that oral administration of aqueous infusions of clove at a dose of 100 microl/mouse/day not only delays the formation of papilloma but also reduces the incidence of papilloma as well as the cumulative number of papillomas per papilloma bearing mouse. Our observations suggest a promising role for cloves in restriction of the carcinogenesis process. JF - Asian Pacific journal of cancer prevention : APJCP AU - Banerjee, Sarmistha AU - Das, Sukta AD - Dept. of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700026, West Bengal, India. rmisti@rediffmail.com PY - 2005 SP - 304 EP - 308 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Carcinogens KW - 0 KW - Plant Extracts KW - 9,10-Dimethyl-1,2-benzanthracene KW - 57-97-6 KW - Index Medicus KW - Administration, Oral KW - Animals KW - Mice KW - Neoplasms, Experimental KW - Skin Neoplasms -- physiopathology KW - Plant Extracts -- pharmacology KW - Papilloma -- prevention & control KW - Papilloma -- physiopathology KW - Cell Transformation, Neoplastic -- drug effects KW - Chemoprevention KW - Skin Neoplasms -- prevention & control KW - Syzygium -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68708457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Anticarcinogenic+effects+of+an+aqueous+infusion+of+cloves+on+skin+carcinogenesis.&rft.au=Banerjee%2C+Sarmistha%3BDas%2C+Sukta&rft.aulast=Banerjee&rft.aufirst=Sarmistha&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=304&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Glutathione S-transferase M1 gene polymorphism in Thai nasopharyngeal carcinoma. AN - 68704152; 16235985 AB - Nasopharyngeal carcinoma (NPC) is a serious health problem in Thailand. It is caused by the combined effects of Epstein-Barr virus (EBV), carcinogens and genetic susceptibility. The glutathione S-transferase M1 gene (GSTM1) encodes a phase II enzyme responsible for detoxifying carcinogenic electrophiles. Polymorphic null forms of the gene GSTM1 lack enzyme activity and have been associated with susceptibility to several cancers including NPC. To examine the association between GSTM1 polymorphism and NPC susceptibility in Thais, GSTM1 genotypes (normal and null genotypes) in 78 NPC patients and 145 age-matched healthy controls were determined using PCR assays. Overall, no statistically significant differences were observed in the frequency of GSTM1 genotypes between cases and controls, nor among NPC patients compared on the basis of sex and clinical stage of disease. Carriers with the GSTM1 null genotype had a 2.9-fold increased risk for NPC of WHO type III when compared to those with GSTM1 normal genotype (P 40, (>45 and (>50 years), the frequencies of GSTM1 null genotype in cases the (>45 and (>50 age groups were significantly different from controls (P45 and (>50 years had a 2-fold and 3-fold increased risk for NPC when compared to those with GSTM1 normal genotype (OR = 2.2, 95% CI = 1.1-4.7 and OR = 3.0, 95% CI = 1.2-7.5). We suggest that GSTM1 polymorphism may be associated with NPC susceptibility in Thais, especially for GSTM1 null genotype carriers of age higher than 45 years. The GSTM1 null genotype may be a useful genetic marker for predicting Thai NPC and for screening of early stages of Thai NPC. JF - Asian Pacific journal of cancer prevention : APJCP AU - Tiwawech, Danai AU - Srivatanakul, Petcharin AU - Karalak, Anant AU - Ishida, Takafumi AD - Research Division, National Cancer Institute, Bangkok 10400, Thailand. tdanai@hotmail.com PY - 2005 SP - 270 EP - 275 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Glutathione Transferase KW - EC 2.5.1.18 KW - glutathione S-transferase M1 KW - Index Medicus KW - Genotype KW - Polymerase Chain Reaction KW - Aged, 80 and over KW - Humans KW - Adult KW - Case-Control Studies KW - Aged KW - Thailand -- epidemiology KW - Middle Aged KW - Male KW - Female KW - Polymorphism, Genetic KW - Nasopharyngeal Neoplasms -- epidemiology KW - Glutathione Transferase -- genetics KW - Genetic Predisposition to Disease KW - Nasopharyngeal Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68704152?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Glutathione+S-transferase+M1+gene+polymorphism+in+Thai+nasopharyngeal+carcinoma.&rft.au=Tiwawech%2C+Danai%3BSrivatanakul%2C+Petcharin%3BKaralak%2C+Anant%3BIshida%2C+Takafumi&rft.aulast=Tiwawech&rft.aufirst=Danai&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=270&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of growth, induction of apoptosis and alteration of gene expression by tea polyphenols in the highly metastatic human lung cancer cell line NCI-H460. AN - 68702286; 16235994 AB - Lung cancer is a complex group of diseases but each lesion is thought to originate from a single mutated progenitor cell. It is evident that multiple genetic changes are involved in the generation of each specific type of lung cancer. Due to the high complexity of these processes and rapid metastasis, treatment of advanced lung cancer, particularly of NSCLCs, is far from satisfactory. Thus, there is a need for innovative strategies for modulation of adverse alteration in protooncogene or tumor suppressor genes so that lung carcinogenesis can be suppressed or delayed. To this end, we have evaluated the effects of tea compounds (theaflavins, epicatechin-gallate and epigallo-catechin-gallate) on proliferation and apoptosis and associated gene expression in a highly metastatic human lung cancer cell line NCI-H460. Significant reduction of cell proliferation, detected in situ by BrdU incorporation, and induction of apoptosis, assessed by the by the TUNEL method, were noted following treatments. Expression of p53, Bcl-2, c-Myc and H-Ras, was localized by immunocytochemistry and analysed by Western blotting. Tea compounds upregulated expression of p53, downregulated expression of Bcl-2 but there was no significant influence on H-ras and c-Myc expressions. It is suggested that tea compounds can influence genetic alteration to disfavour, growth and survival of lung cancer cells. JF - Asian Pacific journal of cancer prevention : APJCP AU - Ganguly, Chaiti AU - Saha, Prosenjit AU - Panda, Chinmay Kr AU - Das, Sukta AD - Dept. of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700026, India. PY - 2005 SP - 326 EP - 331 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Flavonoids KW - 0 KW - Phenols KW - Polyphenols KW - Tea KW - Index Medicus KW - Cell Proliferation -- drug effects KW - In Situ Nick-End Labeling KW - Genes, bcl-2 KW - Blotting, Western KW - Tumor Cells, Cultured KW - Genes, p53 KW - Down-Regulation KW - Humans KW - Apoptosis -- drug effects KW - Immunohistochemistry KW - Phenols -- pharmacology KW - Neoplasm Metastasis KW - Flavonoids -- pharmacology KW - Lung Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68702286?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Inhibition+of+growth%2C+induction+of+apoptosis+and+alteration+of+gene+expression+by+tea+polyphenols+in+the+highly+metastatic+human+lung+cancer+cell+line+NCI-H460.&rft.au=Ganguly%2C+Chaiti%3BSaha%2C+Prosenjit%3BPanda%2C+Chinmay+Kr%3BDas%2C+Sukta&rft.aulast=Ganguly&rft.aufirst=Chaiti&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=326&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Photoactivation of quantum dot fluorescence following endocytosis. AN - 68616810; 16178255 AB - We studied the fluorescence of quantum dots in cells. Coating quantum dots with cationic peptides caused them to be endocytosed and transported to lysosomes. After overnight incubation, their fluorescence apparently dimmed but became markedly "photoactivatable", increasing more than 3-fold within minutes on exposure to bright light, and decaying over hours in the dark. Photoactivation was greater in the presence of water than ethanol, and UV illumination compensated for lack of water during photoactivation. Dimming and photoactivation could affect the use of quantum dots as quantitative probes in vivo and lead to new uses, such as tracking molecular movement. JF - Nano letters AU - Silver, Jonathan AU - Ou, Wu AD - Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. jsilver@nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1445 EP - 1449 VL - 5 IS - 7 SN - 1530-6984, 1530-6984 KW - Fluorescent Dyes KW - 0 KW - Index Medicus KW - Radiation Dosage KW - Animals KW - HeLa Cells KW - Humans KW - Light KW - Dose-Response Relationship, Radiation KW - Cell Line KW - Cricetinae KW - Luminescent Measurements -- methods KW - Kidney -- radiation effects KW - Endosomes -- physiology KW - Photochemistry -- methods KW - Quantum Dots KW - Kidney -- physiology KW - Microscopy, Fluorescence -- methods KW - Endocytosis -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68616810?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nano+letters&rft.atitle=Photoactivation+of+quantum+dot+fluorescence+following+endocytosis.&rft.au=Silver%2C+Jonathan%3BOu%2C+Wu&rft.aulast=Silver&rft.aufirst=Jonathan&rft.date=2005-07-01&rft.volume=5&rft.issue=7&rft.spage=1445&rft.isbn=&rft.btitle=&rft.title=Nano+letters&rft.issn=15306984&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic instability: the dark side of the hypoxic response. AN - 68515597; 15970707 AB - Under low oxygen tension, the activated transcription factor HIF-1alpha upregulates an array of hypoxia-inducible genes via heterodimerization with ARNT and binding to the hypoxia-responsive element in the promoter. Alternatively, HIF-1alpha regulates hypoxia-responsive genes by functionally antagonizing the oncoprotein Myc via protein-protein interactions. This so-called HIF-1alpha-Myc mechanism apparently not only accounts for the gene upregulation, but also for the gene downregulation during hypoxia, depending upon the activating and repressive nature of Myc in gene expression. Indeed, our recent study demonstrated that both mismatch repair genes, MSH2 and MSH6, are inhibited by this mechanism in a p53-dependent manner. In particular, the constitutively bound transcription factor Sp1 serves as a molecular switch by recruiting HIF-1alpha in hypoxia to displace the transcription activator Myc from the promoter. Therefore, our findings shed light on the mechanisms underlying hypoxia-induced genetic instability, an "adverse"effect of the hypoxic response, and yet a germane process to tumor survival and progression. JF - Cell cycle (Georgetown, Tex.) AU - To, Kenneth K W AU - Koshiji, Minori AU - Hammer, Stefanie AU - Huang, L Eric AD - Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 881 EP - 882 VL - 4 IS - 7 KW - HIF1A protein, human KW - 0 KW - Hypoxia-Inducible Factor 1, alpha Subunit KW - MSH2 protein, human KW - EC 3.6.1.3 KW - MutS Homolog 2 Protein KW - Index Medicus KW - Humans KW - Colonic Neoplasms -- metabolism KW - MutS Homolog 2 Protein -- metabolism KW - Hypoxia-Inducible Factor 1, alpha Subunit -- metabolism KW - Cell Hypoxia -- genetics KW - Genomic Instability -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68515597?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.atitle=Genetic+instability%3A+the+dark+side+of+the+hypoxic+response.&rft.au=To%2C+Kenneth+K+W%3BKoshiji%2C+Minori%3BHammer%2C+Stefanie%3BHuang%2C+L+Eric&rft.aulast=To&rft.aufirst=Kenneth+K&rft.date=2005-07-01&rft.volume=4&rft.issue=7&rft.spage=881&rft.isbn=&rft.btitle=&rft.title=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.issn=1551-4005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-04 N1 - Date created - 2005-08-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Rabies: an ancient disease that still prevails. AN - 68491640; 16106083 JF - The Indian journal of medical research AU - Madhusudana, S N AD - Department of Neurovirology National Institute of Mental Health & Neuro Sciences Bangalore 560029, India. mshampur@hotmail.com Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 4 EP - 6 VL - 122 IS - 1 SN - 0971-5916, 0971-5916 KW - Index Medicus KW - Rabies virus -- physiology KW - Clinical Laboratory Techniques KW - Humans KW - Bites and Stings -- complications KW - Survival Analysis KW - Prevalence KW - Rabies -- mortality KW - Rabies -- prevention & control KW - Rabies -- epidemiology KW - Rabies -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68491640?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Indian+journal+of+medical+research&rft.atitle=Rabies%3A+an+ancient+disease+that+still+prevails.&rft.au=Madhusudana%2C+S+N&rft.aulast=Madhusudana&rft.aufirst=S&rft.date=2005-07-01&rft.volume=122&rft.issue=1&rft.spage=4&rft.isbn=&rft.btitle=&rft.title=The+Indian+journal+of+medical+research&rft.issn=09715916&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ten-year follow-up of blood lead levels with medical removal protection of shipyard workers. AN - 68477202; 16100939 AB - This cases report compared the short-term changes of BLL with medical removal intervention and follow-up the long-term changes of BLL afterward. During a physical examination in October 1992, a 44-year old shipyard welder was discovered to have a blood lead level (BLL) of 54.1 microg/dl. It was recommended that the shipyard remove this worker from his workplace. In 1993 the BLLs checked for this worker were 36.7 microg/dl in March and 32.0 microg/dl in April. After six months of medical removal, he returned to initial welding work. In 2002, we collected two blood samples from this worker for analysis in May and October. The results were 30.4 microg/dl and 31.6 microg/dl, respectively. Meanwhile, two other welding workers (case 2 and case 3) with BLLs over 40 microg/dl in the survey conducted at the same shipyard in 1992. It took 4 yr to let BLLs downed to less than 40 microg/dl. However, after the blood lead concentration drops to below 40 microg/dl, 10 yr long-term observation indicates that BLLs reduction level off and do not continue to go down in these three cases. JF - Industrial health AU - Yang, Tsan AU - Tung, Ho-Jui AU - Shyr, Jiann-Chian AU - Lai, Ching-Huang AU - Loh, Ching-Hui AU - Liou, Saou-Hsing AD - School of Public Health, National Defense Medical Center, 161 Minchuan East Road, Sec. 6, Nei-Hu, Taipei, Taiwan, ROC. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 611 EP - 614 VL - 43 IS - 3 SN - 0019-8366, 0019-8366 KW - Lead KW - 2P299V784P KW - Index Medicus KW - Ships KW - Humans KW - Adult KW - Welding KW - Follow-Up Studies KW - Male KW - Occupational Exposure -- prevention & control KW - Lead -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68477202?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Industrial+health&rft.atitle=Ten-year+follow-up+of+blood+lead+levels+with+medical+removal+protection+of+shipyard+workers.&rft.au=Yang%2C+Tsan%3BTung%2C+Ho-Jui%3BShyr%2C+Jiann-Chian%3BLai%2C+Ching-Huang%3BLoh%2C+Ching-Hui%3BLiou%2C+Saou-Hsing&rft.aulast=Yang&rft.aufirst=Tsan&rft.date=2005-07-01&rft.volume=43&rft.issue=3&rft.spage=611&rft.isbn=&rft.btitle=&rft.title=Industrial+health&rft.issn=00198366&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic aspects of pulmonary responses to inhaled pollutants. AN - 68470352; 16092722 AB - Air pollution continues to be a major public health concern in industrialized cities throughout the world. Recent population and epidemiological studies that have associated ozone and particulate exposures with morbidity and mortality outcomes underscore the important detrimental effects of these pollutants on the lung. Inter-individual variation in human responses to air pollutants suggests that some subpopulations are at increased risk to the detrimental effects of pollutant exposure, and it has become clear that genetic background is an important susceptibility factor. Environmental exposures to inhaled pollutants and genetic factors associated with disease risk likely interact in a complex fashion that varies from one population to another. The relationships between the genetic background and disease risk and severity is often evaluated through traditional family-based linkage studies and positional cloning techniques. Case-control studies based on association of disease or disease subphenotypes with candidate genes may have certain advantages over family pedigree studies, and have become useful for understanding complex disease phenotypes. This is based in part on continued development of quantitative analysis and development of mapping technologies. Linkage analyses with genetically standardized animal models are useful to identify genetic determinants of host responses to environmental stimuli. For example, linkage analyses using inbred mice have identified chromosomal segments (quantitative trait loci, QTL) that contain genes that control susceptibility to the lung inflammatory and immune dysfunction responses to ozone, nitrogen dioxide, zinc oxide, and sulfate-associated particles. Candidate genes within the pollutant susceptibility QTLs have been tested for proof-of-concept using gene-targeting and overexpression models. Importantly, significant homology exists between the human and mouse genomes. Therefore, comparative mapping between the human and mouse genomes should yield candidate susceptibility genes that may be tested by association studies in humans. The combined human studies and mouse modeling will provide important insight to understanding genetic factors that contribute to differential susceptibility to pollutants in human populations. JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie AU - Kleeberger, Steven R AD - Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, 111 T.W. Alexander Drive, Bldg 101, Rm. D240, Research Triangle Park, NC 27709, USA. kleeber1@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 147 EP - 153 VL - 57 Suppl 1 SN - 0940-2993, 0940-2993 KW - Air Pollutants KW - 0 KW - Index Medicus KW - Animals KW - Humans KW - Quantitative Trait Loci KW - Disease Models, Animal KW - Mice KW - Genome KW - Lung Diseases -- genetics KW - Lung Diseases -- chemically induced KW - Environmental Illness -- genetics KW - Environmental Illness -- chemically induced KW - Genetic Predisposition to Disease KW - Air Pollutants -- adverse effects KW - Inhalation Exposure -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68470352?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.atitle=Genetic+aspects+of+pulmonary+responses+to+inhaled+pollutants.&rft.au=Kleeberger%2C+Steven+R&rft.aulast=Kleeberger&rft.aufirst=Steven&rft.date=2005-07-01&rft.volume=57+Suppl+1&rft.issue=&rft.spage=147&rft.isbn=&rft.btitle=&rft.title=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.issn=09402993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-08-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - En masse analysis of nascent translation using microarrays. AN - 68432157; 16060370 AB - We report a robust method for studying en masse changes in translation using cDNA arrays. The relative distribution of messenger RNAs (mRNAs) along polysome gradients was monitored by performing cDNA array analysis of each gradient fraction and quantifying the mRNA translational status by regression analysis. Using this strategy to study human carcinoma cells exposed to short-wavelength ultraviolet light (UVC), we identified a subset of 17 translationally induced mRNAs and a subset of 69 translationally repressed mRNAs following UVC irradiation. We describe an effective approach for globally investigating changes in protein biosynthesis. JF - BioTechniques AU - Mazan-Mamczarz, Krystyna AU - Kawai, Tomoko AU - Martindale, Jennifer L AU - Gorospe, Myriam AD - National Institutes of Health, Baltimore, MD, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 61 EP - 2, 64, 66-7 VL - 39 IS - 1 SN - 0736-6205, 0736-6205 KW - Neoplasm Proteins KW - 0 KW - Proteome KW - Index Medicus KW - Gene Expression Regulation, Neoplastic KW - Radiation Dosage KW - Ultraviolet Rays KW - Humans KW - Cell Line, Tumor KW - Proteome -- genetics KW - Protein Modification, Translational -- physiology KW - Colorectal Neoplasms -- metabolism KW - Proteome -- metabolism KW - Oligonucleotide Array Sequence Analysis -- methods KW - Protein Biosynthesis -- physiology KW - Neoplasm Proteins -- metabolism KW - Gene Expression Profiling -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68432157?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BioTechniques&rft.atitle=En+masse+analysis+of+nascent+translation+using+microarrays.&rft.au=Mazan-Mamczarz%2C+Krystyna%3BKawai%2C+Tomoko%3BMartindale%2C+Jennifer+L%3BGorospe%2C+Myriam&rft.aulast=Mazan-Mamczarz&rft.aufirst=Krystyna&rft.date=2005-07-01&rft.volume=39&rft.issue=1&rft.spage=61&rft.isbn=&rft.btitle=&rft.title=BioTechniques&rft.issn=07366205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Scien