TY - CPAPER T1 - Fully Automatic Masking of Displacement Encoded (DENSE) Images T2 - 22nd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2005) AN - 40107065; 4000918 JF - 22nd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2005) AU - Wen, H Y1 - 2005/09/15/ PY - 2005 DA - 2005 Sep 15 KW - Automation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=22nd+Annual+Scientific+Meeting+of+the+European+Society+for+Magnetic+Resonance+in+Medicine+and+Biology+%28ESMRMB+2005%29&rft.atitle=Fully+Automatic+Masking+of+Displacement+Encoded+%28DENSE%29+Images&rft.au=Wen%2C+H&rft.aulast=Wen&rft.aufirst=H&rft.date=2005-09-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=22nd+Annual+Scientific+Meeting+of+the+European+Society+for+Magnetic+Resonance+in+Medicine+and+Biology+%28ESMRMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.esmrmb.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Thrombospondin-1 inhibits endothelial cell responses to nitric oxide in a cGMP-dependent manner. AN - 68585170; 16150726 AB - Redox signaling plays an important role in the positive regulation of angiogenesis by vascular endothelial growth factor, but its role in signal transduction by angiogenesis inhibitors is less clear. Using muscle explants in 3D culture, we found that explants from mice lacking the angiogenesis inhibitor thrombospondin-1 (TSP1) exhibit exaggerated angiogenic responses to an exogenous NO donor, which could be reversed by providing exogenous TSP1. To define the basis for inhibition by TSP1, we examined the effects of TSP1 on several proangiogenic responses of endothelial cells to NO. NO has a biphasic effect on endothelial cell proliferation. The positive effect at low doses of NO is sensitive to inhibition of cGMP signaling and picomolar concentrations of TSP1. NO stimulates both directed (chemotactic) and random (chemokinetic) motility of endothelial cells in a cGMP-dependent manner. TSP1 potently inhibits chemotaxis stimulated by NO. Low doses of NO also stimulate adhesion of endothelial cells on type I collagen in a cGMP-dependent manner. TSP1 potently inhibits this response both upstream and downstream of cGMP. NO-stimulated endothelial cell responses are inhibited by recombinant type 1 repeats of TSP1 and a CD36 agonist antibody but not by the N-terminal portion of TSP1, suggesting that CD36 or a related receptor mediates these effects. These results demonstrate a potent antagonism between TSP1 and proangiogenic signaling downstream of NO. Further elucidation of this inhibitory signaling pathway may identify new molecular targets to regulate pathological angiogenesis. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Isenberg, Jeff S AU - Ridnour, Lisa A AU - Perruccio, Elizabeth M AU - Espey, Michael G AU - Wink, David A AU - Roberts, David D AD - Laboratory of Pathology and Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 SP - 13141 EP - 13146 VL - 102 IS - 37 SN - 0027-8424, 0027-8424 KW - Nitric Oxide Donors KW - 0 KW - Thrombospondin 1 KW - Nitric Oxide KW - 31C4KY9ESH KW - Cyclic GMP KW - H2D2X058MU KW - Index Medicus KW - Cell Movement KW - Animals KW - Neovascularization, Physiologic KW - Umbilical Veins -- cytology KW - Mice KW - Cell Proliferation KW - Mice, Knockout KW - Nitric Oxide Donors -- pharmacology KW - Pectoralis Muscles -- cytology KW - Cells, Cultured KW - Mice, Inbred C57BL KW - Drug Antagonism KW - Cell Adhesion KW - Cyclic GMP -- analysis KW - Thrombospondin 1 -- physiology KW - Endothelium, Vascular -- drug effects KW - Endothelium, Vascular -- cytology KW - Cyclic GMP -- physiology KW - Nitric Oxide -- pharmacology KW - Thrombospondin 1 -- deficiency KW - Thrombospondin 1 -- pharmacology KW - Endothelium, Vascular -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68585170?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Thrombospondin-1+inhibits+endothelial+cell+responses+to+nitric+oxide+in+a+cGMP-dependent+manner.&rft.au=Isenberg%2C+Jeff+S%3BRidnour%2C+Lisa+A%3BPerruccio%2C+Elizabeth+M%3BEspey%2C+Michael+G%3BWink%2C+David+A%3BRoberts%2C+David+D&rft.aulast=Isenberg&rft.aufirst=Jeff&rft.date=2005-09-13&rft.volume=102&rft.issue=37&rft.spage=13141&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-09 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nat Med. 2000 Jan;6(1):41-8 [10613822] Matrix Biol. 2005 Apr;24(2):110-23 [15890262] Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2604-9 [11226286] J Pharmacol Exp Ther. 2001 Dec;299(3):818-24 [11714864] FASEB J. 2002 May;16(7):706-8 [11978735] Methods Enzymol. 2002;352:280-95 [12125354] Am J Physiol Lung Cell Mol Physiol. 2002 Oct;283(4):L671-7 [12225941] Mol Pharmacol. 2002 Oct;62(4):927-35 [12237340] Circulation. 2001 Apr 24;103(16):2102-7 [11319202] Antioxid Redox Signal. 2002 Oct;4(5):783-4 [12470505] Am J Physiol Heart Circ Physiol. 2003 Jan;284(1):H92-H100 [12388327] Matrix Biol. 2003 Mar;22(1):63-71 [12714043] Mol Cell Biol. 2003 Aug;23(16):5726-37 [12897144] Free Radic Biol Med. 2003 Aug 15;35(4):381-96 [12899940] Clin Cancer Res. 2003 Nov 1;9(14):5358-69 [14614021] Int J Biochem Cell Biol. 2004 Mar;36(3):460-71 [14687924] J Biol Chem. 2004 Jan 23;279(4):2550-8 [14600153] Circ Res. 2004 Mar 5;94(4):462-70 [14699013] Biochem Biophys Res Commun. 2004 May 28;318(2):520-8 [15120632] Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8894-9 [15178764] J Biol Chem. 2004 Aug 13;279(33):34311-22 [15184388] Circulation. 2004 Aug 31;110(9):1128-33 [15313948] J Cell Biol. 1989 Sep;109(3):1309-19 [2768342] J Cell Biol. 1990 Aug;111(2):765-72 [1696271] J Cell Biol. 1993 Jul;122(2):497-511 [7686555] J Cell Biochem. 1993 Sep;53(1):74-84 [8227183] J Cell Physiol. 1996 Jul;168(1):217-27 [8647918] J Cell Sci. 1996 Oct;109 ( Pt 10):2499-508 [8923211] J Cell Biol. 1997 Aug 11;138(3):707-17 [9245797] J Pept Res. 1997 Sep;50(3):210-21 [9309585] J Clin Invest. 1997 Dec 15;100(12):3131-9 [9399960] Circ Res. 1998 Feb 9;82(2):139-46 [9468184] Cancer. 1998 Feb 15;82(4):632-8 [9477093] J Clin Invest. 1998 Mar 1;101(5):982-92 [9486968] Proc Natl Acad Sci U S A. 1998 May 26;95(11):6343-8 [9600967] J Clin Invest. 1998 Jun 1;101(11):2567-78 [9616228] Cancer Res. 1998 Jul 15;58(14):3154-62 [9679984] Cancer Res. 1998 Aug 15;58(16):3751-6 [9721889] J Biol Chem. 1998 Sep 4;273(36):23504-8 [9722588] J Lab Clin Med. 1998 Dec;132(6):519-29 [9851743] Nature. 1999 Jun 10;399(6736):597-601 [10376602] Nature. 1999 Jun 10;399(6736):601-5 [10376603] Atherosclerosis. 1999 May;144(1):49-57 [10381277] Curr Pharm Des. 2005;11(7):849-66 [15777239] Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13147-52 [16141331] J Biol Chem. 2000 Aug 18;275(33):25723-32 [10851246] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Deletion of XPC leads to lung tumors in mice and is associated with early events in human lung carcinogenesis. AN - 68585081; 16141330 AB - Chromosome 3p and 1p deletions are among the most frequent genetic changes in human lung cancer and although candidate tumor suppressor genes have been identified in these regions, no causative correlations have been drawn between deletion or mutation of these and lung carcinogenesis. We identify XPC and Gadd45a as genes within each of these regions involved in lung tumor initiation and progression, respectively. One hundred percent of XPC-/- mice develop multiple spontaneous lung tumors with a minority progressing to non-small cell lung adenocarcinoma, occasionally with metastasis to adjacent lymph nodes. Deletion of Gadd45a alone does not lead to increased lung tumors in mice, but coupled with an XPC deletion, it results in lung tumor progression. Analysis of published data indicated allelic loss of XPC in most human lung tumors and allelic loss of Gadd45a in some human lung and other cancer types. Because DNA repair capacity is compromised in XPC+/- cells, it is possible that the loss of a single XPC allele in the human lung might confer a mutator phenotype. Coupled with cigarette carcinogens, decreased DNA repair would lead to additional mutations in genes such as p53 that are frequent targets in lung cancer. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Hollander, M Christine AU - Philburn, Robyn T AU - Patterson, Andrew D AU - Velasco-Miguel, Susana AU - Friedberg, Errol C AU - Linnoila, R Ilona AU - Fornace, Albert J AD - National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. ch96b@nih.gov Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 SP - 13200 EP - 13205 VL - 102 IS - 37 SN - 0027-8424, 0027-8424 KW - Cell Cycle Proteins KW - 0 KW - DNA-Binding Proteins KW - GADD45A protein, human KW - Gadd45a protein, mouse KW - Nuclear Proteins KW - Xpc protein, mouse KW - XPC protein, human KW - 156533-34-5 KW - Index Medicus KW - Chromosomes, Human, Pair 1 KW - Animals KW - Chromosomes, Human, Pair 3 KW - Alleles KW - DNA Damage KW - Humans KW - Mice KW - Chromosome Mapping KW - Male KW - Female KW - Mice, Knockout KW - Carcinoma, Non-Small-Cell Lung -- pathology KW - Cell Cycle Proteins -- physiology KW - Lung Neoplasms -- etiology KW - Nuclear Proteins -- genetics KW - Cell Cycle Proteins -- genetics KW - DNA-Binding Proteins -- genetics KW - Lung Neoplasms -- genetics KW - DNA-Binding Proteins -- physiology KW - Nuclear Proteins -- physiology KW - Lung Neoplasms -- pathology KW - Gene Deletion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68585081?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Deletion+of+XPC+leads+to+lung+tumors+in+mice+and+is+associated+with+early+events+in+human+lung+carcinogenesis.&rft.au=Hollander%2C+M+Christine%3BPhilburn%2C+Robyn+T%3BPatterson%2C+Andrew+D%3BVelasco-Miguel%2C+Susana%3BFriedberg%2C+Errol+C%3BLinnoila%2C+R+Ilona%3BFornace%2C+Albert+J&rft.aulast=Hollander&rft.aufirst=M&rft.date=2005-09-13&rft.volume=102&rft.issue=37&rft.spage=13200&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-09 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mutat Res. 2000 Mar 20;459(2):99-108 [10725660] Nucleic Acids Res. 2002 Aug 15;30(16):3624-31 [12177305] Int J Cancer. 2000 Jun 1;86(5):690-4 [10797292] Cancer Res. 2000 Sep 1;60(17):4894-906 [10987304] Biol Chem. 2002 Jun;383(6):907-14 [12222680] Oncogene. 2002 Oct 7;21(45):6915-35 [12362274] Cancer Biol Ther. 2002 May-Jun;1(3):232-6 [12432269] Cancer Res. 2002 Dec 15;62(24):7305-15 [12499274] Lung Cancer. 2003 Mar;39(3):273-7 [12609565] Cancer Res. 2003 Mar 1;63(5):902-5 [12615700] Mol Biotechnol. 2003 Jun;24(2):141-56 [12746555] Cancer Cell. 2003 Sep;4(3):181-9 [14522252] Mutat Res. 2003 Nov;544(2-3):107-14 [14644313] Annu Rev Physiol. 2004;66:647-63 [14977417] Cancer Res. 2004 Apr 1;64(7):2307-16 [15059877] Cancer Res. 2004 May 1;64(9):3096-102 [15126346] Am J Physiol Lung Cell Mol Physiol. 2004 Oct;287(4):L685-703 [15355860] Cancer Res. 2004 Sep 15;64(18):6432-7 [15374951] Lab Anim. 1971 Oct;5(2):179-92 [5166568] JAMA. 1995 Feb 15;273(7):558-63 [7837389] Curr Biol. 1996 Dec 1;6(12):1691-4 [8994835] Mutat Res. 1997 Mar 4;374(1):1-9 [9067411] Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9463-8 [9256505] Carcinogenesis. 1998 Mar;19(3):501-7 [9525286] Exp Lung Res. 1998 Jul-Aug;24(4):481-97 [9659579] Oncogene. 1998 Jun 11;16(23):3083-6 [9662341] Cancer Res. 1999 Feb 15;59(4):771-5 [10029060] Br J Cancer. 1999 Mar;79(9-10):1468-74 [10188892] Prog Exp Tumor Res. 1999;35:37-52 [10377750] Oncogene. 1999 Sep 20;18(38):5318-24 [10498884] Nat Genet. 1999 Oct;23(2):176-84 [10508513] Cancer Epidemiol Biomarkers Prev. 2004 Nov;13(11 Pt 1):1788-93 [15533908] Cancer Res. 2005 Jan 1;65(1):92-8 [15665283] Mol Carcinog. 2005 Feb;42(2):65-92 [15682379] Genes Dev. 2005 Mar 15;19(6):643-64 [15769940] Int J Cancer. 2005 Jun 20;115(3):478-83 [15700316] Int J Cancer. 2005 Jul 10;115(5):807-13 [15729698] Oncogene. 2000 Oct 12;19(43):5034-7 [11042691] Int J Cancer. 2000 Dec 15;88(6):932-7 [11093817] Cancer Res. 2001 Mar 15;61(6):2487-91 [11289119] Cancer Res. 2001 Apr 15;61(8):3321-5 [11309287] Photodermatol Photoimmunol Photomed. 2001 Apr;17(2):47-54 [11338401] Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10250-5 [11517343] Toxicol Pathol. 2001 Nov-Dec;29(6):653-61 [11794381] Oncogene. 2002 May 2;21(19):3020-8 [12082532] Mol Cell Biol. 2000 May;20(10):3705-14 [10779360] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Cancer Incidence Among Pesticide Applicators Exposed to Metolachlor in the Agricultural Health Study T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39793705; 4025841 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Rusieck, J A AU - Hou, L AU - Lee, W J AU - Blair, A AU - Dosemeci, M AU - Lubin, J AU - Bonner, M AU - Samanic, C AU - Hoppin, J A AU - Sandler, D P AU - Alavanja, M.C.R. Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - Pesticides KW - Agriculture KW - Metolachlor KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39793705?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Cancer+Incidence+Among+Pesticide+Applicators+Exposed+to+Metolachlor+in+the+Agricultural+Health+Study&rft.au=Rusieck%2C+J+A%3BHou%2C+L%3BLee%2C+W+J%3BBlair%2C+A%3BDosemeci%2C+M%3BLubin%2C+J%3BBonner%2C+M%3BSamanic%2C+C%3BHoppin%2C+J+A%3BSandler%2C+D+P%3BAlavanja%2C+M.C.R.&rft.aulast=Rusieck&rft.aufirst=J&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Review of Human Data on Effects of DDT, and Some New Results T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39791633; 4025673 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Longnecker, M P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - DDT KW - Reviews KW - Insecticides KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39791633?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Review+of+Human+Data+on+Effects+of+DDT%2C+and+Some+New+Results&rft.au=Longnecker%2C+M+P&rft.aulast=Longnecker&rft.aufirst=M&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Epidemiologic Evaluation of Measurement Data in the Presence of Detection Limits T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39728252; 4025690 DE: JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Lubin, J H AU - Colt, J S AU - Camann, D AU - Davis, S AU - Cerhan, J R AU - Severson, R K AU - Bernstein, L AU - Hartge, P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39728252?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=Epidemiologic+Evaluation+of+Measurement+Data+in+the+Presence+of+Detection+Limits&rft.au=Lubin%2C+J+H%3BColt%2C+J+S%3BCamann%2C+D%3BDavis%2C+S%3BCerhan%2C+J+R%3BSeverson%2C+R+K%3BBernstein%2C+L%3BHartge%2C+P&rft.aulast=Lubin&rft.aufirst=J&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - An Overview of DDT World Policy T2 - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AN - 39648183; 4025672 JF - 17th Conference of the International Society for Environmental Epidemiology (ISEE 2005) AU - Longnecker, M P Y1 - 2005/09/13/ PY - 2005 DA - 2005 Sep 13 KW - DDT KW - Reviews KW - Insecticides KW - Policies KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39648183?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.atitle=An+Overview+of+DDT+World+Policy&rft.au=Longnecker%2C+M+P&rft.aulast=Longnecker&rft.aufirst=M&rft.date=2005-09-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+Conference+of+the+International+Society+for+Environmental+Epidemiology+%28ISEE+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.epidem.com/pt/re/epidemiology/currenttoc.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Strengthening Institutional Capacity for Effective Research Stewardship T2 - 9th Global Forum for Health Research AN - 39762092; 4045914 DE: JF - 9th Global Forum for Health Research AU - Krotoski, Danuta Y1 - 2005/09/12/ PY - 2005 DA - 2005 Sep 12 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39762092?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=9th+Global+Forum+for+Health+Research&rft.atitle=Strengthening+Institutional+Capacity+for+Effective+Research+Stewardship&rft.au=Krotoski%2C+Danuta&rft.aulast=Krotoski&rft.aufirst=Danuta&rft.date=2005-09-12&rft.volume=122&rft.issue=1-3&rft.spage=741&rft.isbn=&rft.btitle=&rft.title=Applied+Biochemistry+and+Biotechnology&rft.issn=02732289&rft_id=info:doi/10.1385%2FABAB%3A122%3A1-3%3A0741 L2 - http://www.globalforumhealth.org/filesupld/forum9/Edited%20programme%20Fina l.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Role of Stem Cells in Hepatocarcinogenesis T2 - 42nd Congress of the European Societies of Toxicology (EUROTOX 2005) AN - 40055777; 3975930 JF - 42nd Congress of the European Societies of Toxicology (EUROTOX 2005) AU - Thorgeirsson, S S Y1 - 2005/09/11/ PY - 2005 DA - 2005 Sep 11 KW - Stem cells KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40055777?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=42nd+Congress+of+the+European+Societies+of+Toxicology+%28EUROTOX+2005%29&rft.atitle=The+Role+of+Stem+Cells+in+Hepatocarcinogenesis&rft.au=Thorgeirsson%2C+S+S&rft.aulast=Thorgeirsson&rft.aufirst=S&rft.date=2005-09-11&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=42nd+Congress+of+the+European+Societies+of+Toxicology+%28EUROTOX+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eurotox2005.org/eurotox/index.jsp?place=Menu01&news_cat_id=-1&la yout=0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - The common A467T mutation in the human mitochondrial DNA polymerase (POLG) compromises catalytic efficiency and interaction with the accessory subunit. AN - 68571983; 16024923 AB - Among the nearly 50 disease mutations in the gene for the catalytic subunit of human DNA polymerase gamma, POLG, the A467T substitution is the most common and has been found in 0.6% of the Belgian population. The A467T mutation is associated with a wide range of mitochondrial disorders, including Alpers syndrome, juvenile spinocerebellar ataxia-epilepsy syndrome, and progressive external ophthalmoplegia, each with vastly different clinical presentations, tissue specificities, and ages of onset. The A467T mutant enzyme possesses only 4% of wild-type DNA polymerase activity, and the catalytic defect is manifest primarily through a 6-fold reduction in kcat with minimal effect on exonuclease function. Human DNA polymerase gamma (pol gamma) requires association of a 55-kDa accessory subunit for enhanced DNA binding and highly processive DNA synthesis. However, the A467T mutant enzyme failed to interact with and was not stimulated by the accessory subunit, as judged by processivity, heat inactivation, and N-ethylmaleimide protection assays in vitro. Thermolysin digestion and immunoprecipitation experiments further indicate weak association of the subunits for A467T pol gamma. This is the first example of a mutation in POLG that disrupts physical association of the pol gamma subunits. We propose that reduced polymerase activity and loss of accessory subunit interaction are responsible for the depletion and deletion of mitochondrial DNA observed in patients with this POLG mutation. JF - The Journal of biological chemistry AU - Chan, Sherine S L AU - Longley, Matthew J AU - Copeland, William C AD - Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31341 EP - 31346 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - DNA, Mitochondrial KW - 0 KW - Nucleic Acid Synthesis Inhibitors KW - Threonine KW - 2ZD004190S KW - POLG protein, human KW - EC 2.7.7.- KW - DNA-Directed DNA Polymerase KW - EC 2.7.7.7 KW - Thermolysin KW - EC 3.4.24.27 KW - Ethylmaleimide KW - O3C74ACM9V KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Animals KW - Threonine -- genetics KW - Spodoptera KW - Humans KW - Thermolysin -- metabolism KW - Protein Structure, Quaternary KW - Mutagenesis, Site-Directed KW - Hot Temperature KW - Amino Acid Substitution -- genetics KW - Point Mutation KW - Alanine -- genetics KW - Ethylmaleimide -- pharmacology KW - Cell Line KW - DNA, Mitochondrial -- metabolism KW - Catalytic Domain -- genetics KW - DNA-Directed DNA Polymerase -- genetics KW - DNA-Directed DNA Polymerase -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571983?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahnpnewyorktimes&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Television+This+Week%3A+OF+SPECIAL+INTEREST+Today+Monday+Tuesday+Wednesday+Thursday+Saturday+Channel+Information+TODAY--SUNDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+6+Morning+Afternoon+Evening+MONDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+7+Morning+Afternoon+Evening+TUESDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+8+Morning+Afternoon+Evening+WEDNESDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+9+Morning+Afternoon+Evening+THURSDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+10+Morning+Afternoon+Evening+FRIDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+11+Morning+Afternoon+Evening+SATURDAY%2C+%3Cspan+class%3D%22hit%22%3ENOVEMBER%3C%2Fspan%3E+12+Morning+Afternoon+Evening&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1977-11-06&rft.volume=&rft.issue=&rft.spage=D37&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - 15S-Lipoxygenase-2 mediates arachidonic acid-stimulated adhesion of human breast carcinoma cells through the activation of TAK1, MKK6, and p38 MAPK. AN - 68571132; 16000313 AB - The dietary cis-polyunsaturated fatty acid, arachidonic acid, stimulates adhesion of metastatic human breast carcinoma cells (MDA-MB-435) to the extracellular matrix, but the molecular mechanisms by which fatty acids modify the behavior of these cells are unclear. Exposure to arachidonic acid activates multiple signaling pathways. Activation of p38 mitogen-activated protein kinase (p38 MAPK) is required for increased cell adhesion to type IV collagen, and this activation is sensitive to inhibitors of lipoxygenases, suggesting a requirement for arachidonic acid metabolism. The goals of the current study were to identify the one or more key metabolites of arachidonic acid that are responsible for activation of p38 MAPK and to elucidate the upstream kinases that lead to p38 MAPK activation. High performance liquid chromatographic analysis revealed that MDA-MB-435 cells metabolize exogenous arachidonic acid predominantly to 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). Immunoblot analysis with antibodies specific to 15(S)-lipoxygenase-1 (LOX-1) and 15(S)-lipoxygenase-2 (LOX-2) demonstrated the expression of 15-LOX-2, but not 15-LOX-1, in these tumor cells. A LOX inhibitor, nordihydroguaiaretic acid, attenuated production of 15(S)-HETE and inhibited the phosphorylation of p38 MAPK following exposure to arachidonic acid. In contrast, overexpression of LOX-2 sensitized the cells to the addition of arachidonic acid, leading to increased activation of p38 MAPK. Addition of exogenous 15(S)-HETE to MDA-MB-435 cells stimulated cell adhesion to type IV collagen and activated the p38 MAPK pathway, including the upstream kinases transforming growth factor-beta1-activated protein kinase-1 (TAK1) and MAPK kinase 6. Transfection of these cells with a dominant negative form of TAK1 blocked arachidonic acid-stimulated p38 MAPK phosphorylation. These data demonstrate that 15(S)-LOX-2 generation of 15(S)-HETE activates specific growth factor receptor-related signaling pathways, thereby initiating signal transduction events leading to increased cell adhesion to the extracellular matrix. JF - The Journal of biological chemistry AU - Nony, Paul A AU - Kennett, Sarah B AU - Glasgow, Wayne C AU - Olden, Kenneth AU - Roberts, John D AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31413 EP - 31419 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - Hydroxyeicosatetraenoic Acids KW - 0 KW - Lipoxygenase Inhibitors KW - Arachidonic Acid KW - 27YG812J1I KW - Masoprocol KW - 7BO8G1BYQU KW - Arachidonate 15-Lipoxygenase KW - EC 1.13.11.33 KW - p38 Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - MAP Kinase Kinase Kinase 5 KW - EC 2.7.11.25 KW - MAP Kinase Kinase Kinases KW - MAP kinase kinase kinase 7 KW - MAP3K5 protein, human KW - MAP Kinase Kinase 6 KW - EC 2.7.12.2 KW - MAP2K6 protein, human KW - Index Medicus KW - Phosphorylation KW - Cell Adhesion -- physiology KW - Humans KW - MAP Kinase Kinase Kinase 5 -- metabolism KW - Enzyme Activation -- physiology KW - Carcinoma -- enzymology KW - Cell Line, Tumor KW - Hydroxyeicosatetraenoic Acids -- metabolism KW - Female KW - Chromatography, High Pressure Liquid KW - Masoprocol -- pharmacology KW - MAP Kinase Kinase Kinases -- metabolism KW - Arachidonic Acid -- physiology KW - Breast Neoplasms -- enzymology KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Arachidonic Acid -- metabolism KW - MAP Kinase Kinase 6 -- metabolism KW - Arachidonate 15-Lipoxygenase -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=15S-Lipoxygenase-2+mediates+arachidonic+acid-stimulated+adhesion+of+human+breast+carcinoma+cells+through+the+activation+of+TAK1%2C+MKK6%2C+and+p38+MAPK.&rft.au=Nony%2C+Paul+A%3BKennett%2C+Sarah+B%3BGlasgow%2C+Wayne+C%3BOlden%2C+Kenneth%3BRoberts%2C+John+D&rft.aulast=Nony&rft.aufirst=Paul&rft.date=2005-09-09&rft.volume=280&rft.issue=36&rft.spage=31413&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. AN - 68571020; 16002405 AB - DNA polymerase lambda (pol lambda) is a member of the X family of DNA polymerases that has been implicated in both base excision repair and non-homologous end joining through in vitro studies. However, to date, no phenotype has been associated with cells deficient in this DNA polymerase. Here we show that pol lambda null mouse fibroblasts are hypersensitive to oxidative DNA damaging agents, suggesting a role of pol lambda in protection of cells against the cytotoxic effects of oxidized DNA. Additionally, pol lambda co-immunoprecipitates with an oxidized base DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1), and localizes to oxidative DNA lesions in situ. From these data, we conclude that pol lambda protects cells against oxidative stress and suggest that it participates in oxidative DNA damage base excision repair. JF - The Journal of biological chemistry AU - Braithwaite, Elena K AU - Kedar, Padmini S AU - Lan, Li AU - Polosina, Yaroslava Y AU - Asagoshi, Kenjiro AU - Poltoratsky, Vladimir P AU - Horton, Julie K AU - Miller, Holly AU - Teebor, George W AU - Yasui, Akira AU - Wilson, Samuel H AD - Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 SP - 31641 EP - 31647 VL - 280 IS - 36 SN - 0021-9258, 0021-9258 KW - Oxidants KW - 0 KW - 5-hydroxymethyluracil KW - 4433-40-3 KW - Pentoxyl KW - 7LCS1FW4JV KW - DNA Polymerase beta KW - EC 2.7.7.- KW - DNA polymerase beta2 KW - DNA Glycosylases KW - EC 3.2.2.- KW - SMUG1 protein, human KW - Uracil-DNA Glycosidase KW - Index Medicus KW - Oxidation-Reduction KW - Animals KW - Pentoxyl -- pharmacology KW - Pentoxyl -- analogs & derivatives KW - HeLa Cells KW - Humans KW - DNA Glycosylases -- metabolism KW - Oxidants -- chemistry KW - Mice KW - Cell Line KW - DNA Repair -- genetics KW - DNA Repair -- physiology KW - DNA Polymerase beta -- deficiency KW - DNA Damage -- physiology KW - DNA Polymerase beta -- genetics KW - DNA Polymerase beta -- physiology KW - DNA Damage -- genetics KW - Fibroblasts -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68571020?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=DNA+polymerase+lambda+protects+mouse+fibroblasts+against+oxidative+DNA+damage+and+is+recruited+to+sites+of+DNA+damage%2Frepair.&rft.au=Braithwaite%2C+Elena+K%3BKedar%2C+Padmini+S%3BLan%2C+Li%3BPolosina%2C+Yaroslava+Y%3BAsagoshi%2C+Kenjiro%3BPoltoratsky%2C+Vladimir+P%3BHorton%2C+Julie+K%3BMiller%2C+Holly%3BTeebor%2C+George+W%3BYasui%2C+Akira%3BWilson%2C+Samuel+H&rft.aulast=Braithwaite&rft.aufirst=Elena&rft.date=2005-09-09&rft.volume=280&rft.issue=36&rft.spage=31641&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-08 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Neonatal Disconnection of the Hippocampus Alters Prefrontal Cortical Function: Implications for Schizophrenia T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39663745; 3974591 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Lipska, Barbara Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Mental disorders KW - Schizophrenia KW - Neonates KW - Hippocampus KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39663745?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Neonatal+Disconnection+of+the+Hippocampus+Alters+Prefrontal+Cortical+Function%3A+Implications+for+Schizophrenia&rft.au=Lipska%2C+Barbara&rft.aulast=Lipska&rft.aufirst=Barbara&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Neuropeptide Y in Anxiety and Alcoholism T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39663668; 3974582 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Heilig, Markus Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Alcoholism KW - Drug abuse KW - Neuropeptide Y KW - Anxiety KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39663668?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Neuropeptide+Y+in+Anxiety+and+Alcoholism&rft.au=Heilig%2C+Markus&rft.aulast=Heilig&rft.aufirst=Markus&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Galanin; a Novel Therapeutic Target for Stress-Related Disorders? T2 - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AN - 39658626; 3974583 JF - 11th Biennial Meeting of the European Behavioural Pharmacology Society (EBPS 2005) AU - Holmes, Andrew Y1 - 2005/09/09/ PY - 2005 DA - 2005 Sep 09 KW - Galanin KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39658626?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.atitle=Galanin%3B+a+Novel+Therapeutic+Target+for+Stress-Related+Disorders%3F&rft.au=Holmes%2C+Andrew&rft.aulast=Holmes&rft.aufirst=Andrew&rft.date=2005-09-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=11th+Biennial+Meeting+of+the+European+Behavioural+Pharmacology+Society+%28EBPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.barcelona2005.ebps.org/default.asp?id=79&mnu=34 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Synthesis and Structure-Activity Relationship of a Novel Series of Aminoalkylindoles with Potential for Imaging the Neuronal Cannabinoid Receptor by Positron Emission Tomography AN - 19390738; 7158832 AB - A new series of CB sub(1) ligands with high binding affinity (K sub(i) = 0.7-100 nM) and moderate lipophilicity (cLogD sub(7.4)) in the range of 2.1-4.5 has been synthesized. A structure-activity relationship study demonstrated that for the studied set of aminoalkylindoles, the molecular dipole of the ground state conformation within the series was inversely related to the affinity. The racemic ligand with highest affinity (0.7 nM), 3-(4-fluoronaphthoyl) -1-(N-methylpiperidin-2-ylmethyl) indole, was radiolabeled with super(18)F. This radioligand specifically labeled CB sub(1) receptors in mouse brain and accumulated in regions of high versus low CB sub(1) receptor density in a ratio of 1.6. The displaceable radioactivity of one enantiomer in the brains of mice determined in a pretreatment study using the CB sub(1) antagonist N-(piperidinyl)-5-(4-chlorophenyl) -1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (SR141716) was nearly double that of the racemate for the same determination; therefore, the active enantiomer is a candidate for PET studies in animals. A pretreatement study for the other enantiomer found no displaceable radioactivity in the same group of mice; this result suggested the enantiomer was inactive. JF - Journal of Medicinal Chemistry AU - Willis, P G AU - Pavlova, O A AU - Chefer, SI AU - Vaupel, D B AU - Mukhin, A G AU - Horti, A G AD - Neuroimaging Research Branch, Intramural Research Program, National Institute on Drug Abuse, NIH, DHHS, 5500 Nathan Shock Driver, Baltimore, Mayland 21224, USA Y1 - 2005/09/08/ PY - 2005 DA - 2005 Sep 08 SP - 5813 EP - 5822 VL - 48 IS - 18 SN - 0022-2623, 0022-2623 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Brain KW - Enantiomers KW - Indole KW - Receptor density KW - Positron emission tomography KW - Radioactivity KW - Cannabinoid CB1 receptors KW - Structure-activity relationships KW - Conformation KW - W 30910:Imaging KW - N3 11008:Neurochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19390738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Synthesis+and+Structure-Activity+Relationship+of+a+Novel+Series+of+Aminoalkylindoles+with+Potential+for+Imaging+the+Neuronal+Cannabinoid+Receptor+by+Positron+Emission+Tomography&rft.au=Willis%2C+P+G%3BPavlova%2C+O+A%3BChefer%2C+SI%3BVaupel%2C+D+B%3BMukhin%2C+A+G%3BHorti%2C+A+G&rft.aulast=Willis&rft.aufirst=P&rft.date=2005-09-08&rft.volume=48&rft.issue=18&rft.spage=5813&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm0502743 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Enantiomers; Cannabinoid CB1 receptors; Brain; Radioactivity; Structure-activity relationships; Positron emission tomography; Neuroimaging; Conformation; Receptor density; Indole DO - http://dx.doi.org/10.1021/jm0502743 ER - TY - JOUR T1 - Reduced Cocaine Self-Administration in Muscarinic M sub(5) Acetylcholine Receptor-Deficient Mice AN - 17404556; 6529455 AB - The reinforcing effects of cocaine have been related to increased extracellular concentrations of dopamine in the ventral striatum. Several studies suggest that M sub(5) muscarinic receptors facilitate striatal dopamine release. We tested the hypothesis that the reinforcing effects of cocaine are decreased in M sub(5) receptor-deficient mice using chronic intravenous cocaine self-administration in extensively backcrossed mice. We also assessed whether operant performance generally, rather than cocaine self-administration specifically, was altered in the mutant mice. To this end, we evaluated both food-maintained operant behavior and cocaine self-administration under a fixed ratio 1 and a progressive ratio (PR) schedule of reinforcement. We also evaluated acquisition of self-administration in experimentally naive mice using several doses of cocaine. M sub(5) receptor deletion decreased self-administration of low to moderate doses of cocaine under a PR schedule of reinforcement and diminished acquisition of self-administration of a low dose in experimentally naive mice. We found no differences between genotypes in food-maintained behavior. The present study extends our previous findings using backcrossed mice and covering various experimental conditions. Our results indicate that M sub(5) receptor deletion diminished the reinforcing effects of low doses of cocaine and identified specific conditions under which this may be observed. JF - Journal of Neuroscience AU - Thomsen, Morgane AU - Woldbye, David PD AU - Woertwein, Gitta AU - Fink-Jensen, Anders AU - Wess, Juergen AU - Caine, SBarak AD - Alcohol and Drug Abuse Research Center, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, Laboratory of Neuropsychiatry, Rigshospitalet University Hospital and Department of Pharmacology, University of Copenhagen, DK-2100 Copenhagen, Denmark, and Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/09/07/ PY - 2005 DA - 2005 Sep 07 SP - 8141 EP - 8149 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 36 SN - 0270-6474, 0270-6474 KW - CSA Neurosciences Abstracts; Toxicology Abstracts KW - Dopamine KW - Neostriatum KW - Acetylcholine receptors (muscarinic) KW - Reinforcement KW - Genotypes KW - Cocaine KW - Drug addiction KW - Drug self-administration KW - X 24180:Social poisons & drug abuse KW - N3 11139:Toxicological and psychoactive drug correlates UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17404556?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Reduced+Cocaine+Self-Administration+in+Muscarinic+M+sub%285%29+Acetylcholine+Receptor-Deficient+Mice&rft.au=Thomsen%2C+Morgane%3BWoldbye%2C+David+PD%3BWoertwein%2C+Gitta%3BFink-Jensen%2C+Anders%3BWess%2C+Juergen%3BCaine%2C+SBarak&rft.aulast=Thomsen&rft.aufirst=Morgane&rft.date=2005-09-07&rft.volume=25&rft.issue=36&rft.spage=8141&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Dopamine; Acetylcholine receptors (muscarinic); Neostriatum; Reinforcement; Genotypes; Drug addiction; Cocaine; Drug self-administration ER - TY - JOUR T1 - Inhibiting farnesylation of progerin prevents the characteristic nuclear blebbing of Hutchinson-Gilford progeria syndrome. AN - 68565457; 16129833 AB - Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is characterized by dramatic premature aging and accelerated cardiovascular disease. HGPS is almost always caused by a de novo point mutation in the lamin A gene (LMNA) that activates a cryptic splice donor site, producing a truncated mutant protein termed "progerin." WT prelamin A is anchored to the nuclear envelope by a farnesyl isoprenoid lipid. Cleavage of the terminal 15 aa and the farnesyl group releases mature lamin A from this tether. In contrast, this cleavage site is deleted in progerin. We hypothesized that retention of the farnesyl group causes progerin to become permanently anchored in the nuclear membrane, disrupting proper nuclear scaffolding and causing the characteristic nuclear blebbing seen in HGPS cells. Also, we hypothesized that blocking farnesylation would decrease progerin toxicity. To test this hypothesis, the terminal CSIM sequence in progerin was mutated to SSIM, a sequence that cannot be farnesylated. SSIM progerin relocalized from the nuclear periphery into nucleoplasmic aggregates and produced no nuclear blebbing. Also, blocking farnesylation of authentic progerin in transiently transfected HeLa, HEK 293, and NIH 3T3 cells with farnesyltransferase inhibitors (FTIs) restored normal nuclear architecture. Last, treatment of both early- and late-passage human HGPS fibroblasts with FTIs resulted in significant reductions in nuclear blebbing. Our results suggest that treatment with FTIs represents a potential therapy for patients with HGPS. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Capell, Brian C AU - Erdos, Michael R AU - Madigan, James P AU - Fiordalisi, James J AU - Varga, Renee AU - Conneely, Karen N AU - Gordon, Leslie B AU - Der, Channing J AU - Cox, Adrienne D AU - Collins, Francis S AD - Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, 50 South Drive, MSC 8004, Bethesda, MD 20892-8004, USA. Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 12879 EP - 12884 VL - 102 IS - 36 SN - 0027-8424, 0027-8424 KW - Enzyme Inhibitors KW - 0 KW - Lamin Type A KW - Alkyl and Aryl Transferases KW - EC 2.5.- KW - Farnesyltranstransferase KW - EC 2.5.1.29 KW - Index Medicus KW - Protein Prenylation -- drug effects KW - Animals KW - Humans KW - Mutation -- genetics KW - Enzyme Inhibitors -- pharmacology KW - Alkyl and Aryl Transferases -- antagonists & inhibitors KW - Mice KW - Cell Line KW - Alkyl and Aryl Transferases -- metabolism KW - Lamin Type A -- chemistry KW - Cell Nucleus -- pathology KW - Progeria -- pathology KW - Progeria -- metabolism KW - Cell Nucleus -- metabolism KW - Lamin Type A -- genetics KW - Lamin Type A -- metabolism KW - Progeria -- drug therapy KW - Cell Nucleus -- drug effects KW - Progeria -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68565457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Inhibiting+farnesylation+of+progerin+prevents+the+characteristic+nuclear+blebbing+of+Hutchinson-Gilford+progeria+syndrome.&rft.au=Capell%2C+Brian+C%3BErdos%2C+Michael+R%3BMadigan%2C+James+P%3BFiordalisi%2C+James+J%3BVarga%2C+Renee%3BConneely%2C+Karen+N%3BGordon%2C+Leslie+B%3BDer%2C+Channing+J%3BCox%2C+Adrienne+D%3BCollins%2C+Francis+S&rft.aulast=Capell&rft.aufirst=Brian&rft.date=2005-09-06&rft.volume=102&rft.issue=36&rft.spage=12879&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-09-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Pathol. 2004 Nov;204(4):478-88 [15495262] Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8963-8 [15184648] Arch Pathol Lab Med. 1981 Jul;105(7):384-6 [6894691] Development. 1989 Feb;105(2):365-78 [2680424] J Cell Biol. 1990 May;110(5):1489-99 [2335559] Cell. 1990 Jul 13;62(1):81-8 [2194674] J Cell Biol. 1993 Nov;123(3):501-12 [8227121] J Cell Sci. 1994 Apr;107 ( Pt 4):1019-29 [8056827] J Biol Chem. 1995 Dec 22;270(51):30611-8 [8530497] Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4454-8 [8633088] J Biol Chem. 1997 Apr 11;272(15):10232-9 [9092572] J Biol Chem. 1997 May 30;272(22):14093-7 [9162034] J Biol Chem. 1997 May 30;272(22):14459-64 [9162087] J Biol Chem. 1998 Jun 12;273(24):15030-4 [9614111] Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18111-6 [15608054] Nat Rev Mol Cell Biol. 2005 Jan;6(1):21-31 [15688064] Nat Med. 2005 Apr;11(4):440-5 [15750600] Curr Opin Drug Discov Devel. 2004 Jul;7(4):478-86 [15338957] Hum Mol Genet. 2004 Oct 15;13(20):2493-503 [15317753] J Cell Biol. 1999 Nov 29;147(5):913-20 [10579712] Postgrad Med J. 2001 May;77(907):312-7 [11320273] Curr Med Chem. 2001 Oct;8(12):1419-36 [11562275] Genes Dev. 2002 Mar 1;16(5):533-47 [11877373] Nat Genet. 2002 May;31(1):94-9 [11923874] Curr Opin Pharmacol. 2002 Aug;2(4):388-93 [12127871] Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13049-54 [12235369] Nature. 2003 May 15;423(6937):293-8 [12714972] Curr Top Med Chem. 2003;3(10):1103-14 [12769711] Curr Opin Genet Dev. 2003 Jun;13(3):223-30 [12787783] Science. 2003 Jun 27;300(5628):2055 [12702809] Hum Mol Genet. 2003 Aug 15;12(16):1995-2001 [12913070] Nat Rev Cancer. 2003 Dec;3(12):945-51 [14737124] J Pediatr. 1972 Apr;80(4):697-724 [4552697] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Clusters of mutations from transient hypermutability. AN - 68564476; 16118275 AB - Collections of mutants usually contain more mutants bearing multiple mutations than expected from the mutant frequency and a random distribution of mutations. This excess is seen in a variety of organisms and also after DNA synthesis in vitro. The excess is unlikely to originate in mutator mutants but rather from transient hypermutability resulting from a perturbation of one of the many transactions that maintain genetic fidelity. The multiple mutations are sometimes clustered and sometimes randomly distributed. We model some spectra as populations comprising a majority with a low mutation frequency and a minority with a high mutation frequency. In the case of mutants produced in vitro by a bacteriophage RB69 mutator DNA polymerase, mutants with two mutations are in approximately 10-fold excess and mutants with three mutations are in even greater excess. However, phenotypically undetectable mutations seen only as hitchhikers with detectable mutations are approximately 5-fold more frequent than mutants bearing detectable mutations, indicating that they arose in a subpopulation with a higher mutation frequency. Excess multiple mutations may contribute critically to carcinogenesis and to adaptive mutation, including the adaptations of pathogens as they move from host to host. In the case of the rapidly mutating riboviruses, the viral population appears to be composed of a majority with a mutation frequency substantially lower than the average and a minority with a huge mutational load. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Drake, John W AU - Bebenek, Anna AU - Kissling, Grace E AU - Peddada, Shyamal AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. drake@niehs.nih.gov Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 12849 EP - 12854 VL - 102 IS - 36 SN - 0027-8424, 0027-8424 KW - RNA KW - 63231-63-0 KW - DNA-Directed DNA Polymerase KW - EC 2.7.7.7 KW - Index Medicus KW - Bacteriophages -- enzymology KW - Animals KW - Humans KW - DNA Mutational Analysis KW - Genome KW - Neoplasms -- genetics KW - RNA -- genetics KW - DNA-Directed DNA Polymerase -- metabolism KW - Mutation -- genetics KW - Mutagenesis -- genetics KW - Evolution, Molecular UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68564476?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Clusters+of+mutations+from+transient+hypermutability.&rft.au=Drake%2C+John+W%3BBebenek%2C+Anna%3BKissling%2C+Grace+E%3BPeddada%2C+Shyamal&rft.aulast=Drake&rft.aufirst=John&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=40&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-09-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Gen Genet. 1984;198(2):177-8 [6394963] Genetics. 2005 Apr;169(4):1815-24 [15695359] Proc Natl Acad Sci U S A. 1987 May;84(10):3354-8 [3554239] Proc Natl Acad Sci U S A. 1988 Nov;85(21):8126-30 [3054881] J Biol Chem. 1989 Oct 5;264(28):16948-56 [2476448] J Mol Biol. 1989 Sep 20;209(2):195-204 [2685319] J Bacteriol. 1990 Jun;172(6):3009-14 [2160935] J Virol. 1990 Aug;64(8):3960-2 [1695258] Genetics. 1991 Nov;129(3):957-62 [1752431] J Mol Biol. 1991 Dec 20;222(4):925-36 [1762158] Mol Gen Genet. 1992 Nov;235(2-3):173-8 [1465091] Genetics. 1993 Aug;134(4):1031-8 [8375646] Nucleic Acids Res. 1993 Nov 11;21(22):5212-20 [7504813] Genetics. 1994 Oct;138(2):263-70 [7828810] Methods Enzymol. 1995;262:217-32 [8594349] Science. 1996 Nov 15;274(5290):1208-11 [8895473] J Bacteriol. 1997 Jan;179(2):417-22 [8990293] J Biol Chem. 1997 Mar 14;272(11):7345-51 [9054433] Nature. 1997 Jun 12;387(6634):700-2 [9192893] EMBO J. 1997 Jun 2;16(11):3303-11 [9214645] Science. 1997 Sep 5;277(5331):1523-6 [9278518] Environ Mol Mutagen. 1997;30(3):273-86 [9366905] Biochemistry. 1998 Feb 24;37(8):2111-9 [9485358] J Mol Biol. 1998 Apr 24;278(1):135-46 [9571039] Genetics. 1998 Apr;148(4):1483-90 [9560368] Genetics. 1998 Apr;148(4):1619-26 [9560381] Genetics. 1998 Apr;148(4):1667-86 [9560386] Mutat Res. 1998 May 25;400(1-2):89-97 [9685594] Mutagenesis. 1998 Sep;13(5):487-97 [9800194] Science. 1999 Jan 29;283(5402):641 [9988656] Environ Mol Mutagen. 1999;33(2):132-43 [10217067] Genetics. 1999 Jun;152(2):485-93 [10353893] Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13910-3 [10570172] Genetics. 2000 Mar;154(3):959-70 [10757746] Science. 2000 Apr 21;288(5465):514-7 [10775110] Mutat Res. 2000 Sep 18;452(2):219-29 [11024481] Mutat Res. 2000 Dec 20;457(1-2):93-104 [11106801] J Biol Chem. 2001 Mar 30;276(13):10387-97 [11133987] Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7928-33 [11427720] Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1437-42 [11818556] J Virol. 2002 Jun;76(11):5822-8 [11992012] J Biol Chem. 2002 Sep 20;277(38):35550-60 [12121998] Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14878-83 [12403824] Genetics. 2002 Nov;162(3):1003-18 [12454051] Mutat Res. 2002 Dec 29;510(1-2):153-68 [12459451] Genetics. 2002 Dec;162(4):1505-11 [12524327] J Virol. 2003 Mar;77(5):2946-55 [12584319] Cold Spring Harb Symp Quant Biol. 2000;65:21-9 [12760017] Mutat Res. 2004 Oct 4;554(1-2):223-40 [15450421] Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6862-7 [10359804] J Virol. 1999 Jul;73(7):5326-32 [10364279] Acta Pathol Microbiol Scand. 1960;48:113-20 [14408281] J Gen Microbiol. 1959 Dec;21:530-49 [14440412] J Biol Chem. 1985 May 10;260(9):5787-96 [3988773] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Roles of Drosophila DJ-1 in survival of dopaminergic neurons and oxidative stress. AN - 68552430; 16139214 AB - The loss of dopaminergic neurons in the substantia nigra is the pathological hallmark of Parkinson's disease (PD). While the etiology of sporadic PD remains elusive, an inherited form of early-onset familial PD is linked to mutations of DJ-1. To understand the biological function of DJ-1 and its relevance to the pathogenesis of PD, we investigated the function of DJ-1 using Drosophila. Drosophila possesses two homologs of human DJ-1: DJ-1alpha and DJ-1beta. We found that DJ-1alpha is expressed predominantly in the testis, while DJ-1beta is ubiquitously present in most tissues, resembling the expression pattern of human DJ-1. Loss-of-function DJ-1beta mutants demonstrated an extended survival of dopaminergic neurons and resistance to paraquat stress, but showed acute sensitivity to hydrogen peroxide treatment. We showed a compensatory upregulation of DJ-1alpha expression in the brain of the DJ-1beta mutant and demonstrated that overexpression of DJ-1alpha in dopaminergic neurons is sufficient to confer protection against paraquat insult. These results suggest that Drosophila homologs of DJ-1 play critical roles in the survival of dopaminergic neurons and response to oxidative stress. JF - Current biology : CB AU - Menzies, Fiona M AU - Yenisetti, Sarat C AU - Min, Kyung-Tai AD - Neurogenetics Branch MSC 3705, Building 35, Room 2A 1002 NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 SP - 1578 EP - 1582 VL - 15 IS - 17 SN - 0960-9822, 0960-9822 KW - DJ-1alpha protein, Drosophila KW - 0 KW - DJ-1beta protein, Drosophila KW - Drosophila Proteins KW - Nerve Tissue Proteins KW - Hydrogen Peroxide KW - BBX060AN9V KW - Paraquat KW - PLG39H7695 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Hydrogen Peroxide -- toxicity KW - Gene Expression Profiling KW - Animals KW - Blotting, Western KW - Sequence Alignment KW - Molecular Sequence Data KW - Mutation -- genetics KW - Dopamine -- metabolism KW - Brain -- metabolism KW - Amino Acid Sequence KW - Immunohistochemistry KW - Paraquat -- toxicity KW - Neurons -- metabolism KW - Neurons -- drug effects KW - Oxidative Stress -- drug effects KW - Drosophila -- metabolism KW - Nerve Tissue Proteins -- metabolism KW - Oxidative Stress -- genetics KW - Gene Expression Regulation -- drug effects KW - Drosophila Proteins -- genetics KW - Nerve Tissue Proteins -- genetics KW - Drosophila -- genetics KW - Drosophila Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68552430?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+biology+%3A+CB&rft.atitle=Roles+of+Drosophila+DJ-1+in+survival+of+dopaminergic+neurons+and+oxidative+stress.&rft.au=Menzies%2C+Fiona+M%3BYenisetti%2C+Sarat+C%3BMin%2C+Kyung-Tai&rft.aulast=Menzies&rft.aufirst=Fiona&rft.date=2005-09-06&rft.volume=15&rft.issue=17&rft.spage=1578&rft.isbn=&rft.btitle=&rft.title=Current+biology+%3A+CB&rft.issn=09609822&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-23 N1 - Date created - 2005-09-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Pancreatic Cancer and Neural System Tumors (NSTS) in Melanoma-Prone Families with CDKN2A Mutations T2 - 6th World Congress on Melanoma AN - 40116180; 3994356 JF - 6th World Congress on Melanoma AU - Goldstein, A Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Mutation KW - Pancreatic cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40116180?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Pancreatic+Cancer+and+Neural+System+Tumors+%28NSTS%29+in+Melanoma-Prone+Families+with+CDKN2A+Mutations&rft.au=Goldstein%2C+A&rft.aulast=Goldstein&rft.aufirst=A&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Surgical Treatment in Horizontal Growth Phase Melanoma. Results of a Prospective Study at National Cancer Institute, Milan T2 - 6th World Congress on Melanoma AN - 40115246; 3994088 JF - 6th World Congress on Melanoma AU - Moglia, D AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Maurichi, A AU - Patuzzo, R AU - Pennacchioli, E AU - Santoro, N AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40115246?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Surgical+Treatment+in+Horizontal+Growth+Phase+Melanoma.+Results+of+a+Prospective+Study+at+National+Cancer+Institute%2C+Milan&rft.au=Moglia%2C+D%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BLiborio%2C+F%3BMaurichi%2C+A%3BPatuzzo%2C+R%3BPennacchioli%2C+E%3BSantoro%2C+N%3BSantinami%2C+M&rft.aulast=Moglia&rft.aufirst=D&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Radiofrequency Thermal Ablation of Liver Melanoma Metastases. A Study of 19 Cases T2 - 6th World Congress on Melanoma AN - 40082041; 3994193 JF - 6th World Congress on Melanoma AU - Maurichi, A AU - Bono, A AU - Bartoli, C AU - Liborio, F AU - Moglia, D AU - Patuzzo, R AU - Pennacchioli, E AU - Rebuffoni, G AU - Santinami, M AU - Garbagnati, F Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Ablation KW - Metastases KW - Melanoma KW - Liver KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40082041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Legal+Notice+2+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2000-11-29&rft.volume=&rft.issue=&rft.spage=G7&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Interplay of MC1R, ASIP, and DNA Repair in Sporadic and Familial Melanoma Risk in a Mediterranean population T2 - 6th World Congress on Melanoma AN - 40081760; 3994142 JF - 6th World Congress on Melanoma AU - Landi, M T AU - Kanetsky, P A AU - Tsang, S AU - Gold, B AU - Rebbeck, T AU - Hedayati, M AU - Grossman, L AU - Goldstein, A M AU - Calista, D AU - Pfeiffer, R M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Mediterranean KW - Melanoma KW - Mediterranean region KW - DNA repair KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40081760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Interplay+of+MC1R%2C+ASIP%2C+and+DNA+Repair+in+Sporadic+and+Familial+Melanoma+Risk+in+a+Mediterranean+population&rft.au=Landi%2C+M+T%3BKanetsky%2C+P+A%3BTsang%2C+S%3BGold%2C+B%3BRebbeck%2C+T%3BHedayati%2C+M%3BGrossman%2C+L%3BGoldstein%2C+A+M%3BCalista%2C+D%3BPfeiffer%2C+R+M&rft.aulast=Landi&rft.aufirst=M&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Predicting Absolute Risk of Melanoma: A Management Model for Use by Primary Care Providers T2 - 6th World Congress on Melanoma AN - 40078679; 3994451 JF - 6th World Congress on Melanoma AU - Fears, T R AU - Guerry AU - Pfeiffer, R M AU - Sagebiel, R W AU - Elder, D E AU - Halpern, A AU - Holly, E A AU - Hartge, P Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Models KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40078679?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+308+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2002-11-10&rft.volume=&rft.issue=&rft.spage=K12&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Radical Dissectione of Groin Basin After Positive SNB: Overtreatment or Standard Surgery? T2 - 6th World Congress on Melanoma AN - 40068300; 3994468 JF - 6th World Congress on Melanoma AU - Pennacchioli, E AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Ruggeri, R AU - Tragni, G AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Surgery KW - Basins KW - Groynes KW - Radicals KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40068300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Radical+Dissectione+of+Groin+Basin+After+Positive+SNB%3A+Overtreatment+or+Standard+Surgery%3F&rft.au=Pennacchioli%2C+E%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BMaurichi%2C+A%3BMoglia%2C+D%3BPatuzzo%2C+R%3BRuggeri%2C+R%3BTragni%2C+G%3BSantinami%2C+M&rft.aulast=Pennacchioli&rft.aufirst=E&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - KIR 2DS4 Genotypes in Sporadic and Familial Melanoma from Italy T2 - 6th World Congress on Melanoma AN - 40041699; 3994442 JF - 6th World Congress on Melanoma AU - Landi, M T AU - Hulley, B AU - Martin, P AU - Pfeiffer, R AU - Goldstein, A AU - Landi, G AU - Carrington, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Italy KW - Melanoma KW - Genotypes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041699?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+67+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1997-05-11&rft.volume=&rft.issue=&rft.spage=H47&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Etiologic and Other Factors Predicting Nevus-Associated Cutaneous Malignant Melanoma T2 - 6th World Congress on Melanoma AN - 40041638; 3994431 JF - 6th World Congress on Melanoma AU - Purdue, M AU - From, L AU - Armstrong, B AU - Kricker, A AU - Gallagher, R AU - McLaughlin, J AU - Klar, N AU - Marrett, L Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041638?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Etiologic+and+Other+Factors+Predicting+Nevus-Associated+Cutaneous+Malignant+Melanoma&rft.au=Poggi%2C+Sarah+H%3BPark%2C+Jane%3BToso%2C+Laura%3BAbebe%2C+Daniel%3BRoberson%2C+Robin%3BWoodard%2C+Jade+E%3BSpong%2C+Catherine+Y&rft.aulast=Poggi&rft.aufirst=Sarah&rft.date=2005-03-01&rft.volume=192&rft.issue=3&rft.spage=727&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=00029378&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Morphologic Evaluation of the Sentinel Node Does Not Correlate with Survival of Melanoma Patients T2 - 6th World Congress on Melanoma AN - 40039922; 3994275 JF - 6th World Congress on Melanoma AU - Santinami, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Roberto, M AU - Tragni, G AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Nodes KW - Survival KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40039922?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=Display+Ad+81+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1988-09-30&rft.volume=&rft.issue=&rft.spage=D16&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Cutaneous Desmoplastic Melanoma T2 - 6th World Congress on Melanoma AN - 40037399; 3994087 JF - 6th World Congress on Melanoma AU - Maurichi, A AU - Baldi, M AU - Bartoli, C AU - Bono, A AU - Liborio, F AU - Moglia, D AU - Patuzzo, R AU - Pennacchioli, E AU - Tragni, G AU - Santinami, M Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40037399?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Cutaneous+Desmoplastic+Melanoma&rft.au=Maurichi%2C+A%3BBaldi%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BLiborio%2C+F%3BMoglia%2C+D%3BPatuzzo%2C+R%3BPennacchioli%2C+E%3BTragni%2C+G%3BSantinami%2C+M&rft.aulast=Maurichi&rft.aufirst=A&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Regional Perfusion for Limbs' in Transit Metastases from Melanoma: Results in a Single Institution T2 - 6th World Congress on Melanoma AN - 40034500; 3994198 JF - 6th World Congress on Melanoma AU - Patuzzo, R AU - Bartoli, C AU - Bono, A AU - Deraco, M AU - Liborio, F AU - Maurichi, A AU - Moglia, D AU - Roberto, M AU - Santinami, M AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - Limbs KW - Perfusion KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40034500?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281923-Current+file%29&rft.atitle=DEATHS&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2008-11-12&rft.volume=&rft.issue=&rft.spage=B19&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281923-Current+file%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Melanoma in Colombia T2 - 6th World Congress on Melanoma AN - 40033972; 3994124 JF - 6th World Congress on Melanoma AU - Acosta, A Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Colombia KW - Melanoma KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40033972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahnpnewyorktimes&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=New+York+Times+%281857-1922%29&rft.atitle=Display+Ad+15+--+No+Title&rft.au=&rft.aulast=&rft.aufirst=&rft.date=1902-11-25&rft.volume=&rft.issue=&rft.spage=13&rft.isbn=&rft.btitle=&rft.title=New+York+Times+%281857-1922%29&rft.issn=03624331&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Sentinel Node Biopsy: Standard Treatment for Melanoma? Results at the National Cancer Institute of Milan T2 - 6th World Congress on Melanoma AN - 40033767; 3994091 JF - 6th World Congress on Melanoma AU - Santinami, M AU - Bartoli, C AU - Bono, A AU - Costanzo, P AU - Maurichi, A AU - Moglia, D AU - Patuzzo, R AU - Santoro, N AU - Tragni, G AU - Pennacchioli, E Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Melanoma KW - Cancer KW - Biopsy KW - Nodes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40033767?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Sentinel+Node+Biopsy%3A+Standard+Treatment+for+Melanoma%3F+Results+at+the+National+Cancer+Institute+of+Milan&rft.au=Santinami%2C+M%3BBartoli%2C+C%3BBono%2C+A%3BCostanzo%2C+P%3BMaurichi%2C+A%3BMoglia%2C+D%3BPatuzzo%2C+R%3BSantoro%2C+N%3BTragni%2C+G%3BPennacchioli%2C+E&rft.aulast=Santinami&rft.aufirst=M&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Adoptive Cell Transfer Therapy Following Lymphodepleting Chemotherapy for the Treatment of Patients with Refractory Metastatic Melanoma T2 - 6th World Congress on Melanoma AN - 40032078; 3994338 JF - 6th World Congress on Melanoma AU - Powell Jr, D AU - Rosenberg, S Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - Chemotherapy KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40032078?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Adoptive+Cell+Transfer+Therapy+Following+Lymphodepleting+Chemotherapy+for+the+Treatment+of+Patients+with+Refractory+Metastatic+Melanoma&rft.au=Powell+Jr%2C+D%3BRosenberg%2C+S&rft.aulast=Powell+Jr&rft.aufirst=D&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Temozolomide for the Treatment of Brain and Systemic Disease in Patients with Metastatic Melanoma. National Cancer Institute Slovakia-The First T2 - 6th World Congress on Melanoma AN - 40012962; 3994202 JF - 6th World Congress on Melanoma AU - Salek, T Y1 - 2005/09/06/ PY - 2005 DA - 2005 Sep 06 KW - Metastases KW - Melanoma KW - temozolomide KW - Brain KW - Cancer KW - Disease control KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40012962?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+World+Congress+on+Melanoma&rft.atitle=Temozolomide+for+the+Treatment+of+Brain+and+Systemic+Disease+in+Patients+with+Metastatic+Melanoma.+National+Cancer+Institute+Slovakia-The+First&rft.au=Salek%2C+T&rft.aulast=Salek&rft.aufirst=T&rft.date=2005-09-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+World+Congress+on+Melanoma&rft.issn=&rft_id=info:doi/ L2 - http://www.venuewest.com/worldmelanoma/images/6th_Melanoma_Web_Final.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - 'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system AN - 17393636; 6513060 AB - DNA damage recognition represents a long-standing problem in the field of protein-DNA interactions. This article reviews our current knowledge of how damage recognition is achieved in bacterial nucleotide excision repair through the concerted action of the UvrA, UvrB, and UvrC proteins. JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis AU - Van Houten, B AU - Croteau, D L AU - DellaVecchia, MJ AU - Wang, H AU - Kisker, C AD - National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD D3-01, Research Triangle Park, NC 27709, USA, vanhout1@niehs.nih.gov Y1 - 2005/09/04/ PY - 2005 DA - 2005 Sep 04 SP - 92 EP - 117 VL - 577 IS - 1-2 SN - 0027-5107, 0027-5107 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - DNA damage KW - Molecular modelling KW - Nucleotide excision repair KW - Reviews KW - Nuclease KW - Mutagenesis KW - J 02310:Genetics & Taxonomy KW - N 14100:Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17393636?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.atitle=%27Close-fitting+sleeves%27%3A+DNA+damage+recognition+by+the+UvrABC+nuclease+system&rft.au=Van+Houten%2C+B%3BCroteau%2C+D+L%3BDellaVecchia%2C+MJ%3BWang%2C+H%3BKisker%2C+C&rft.aulast=Van+Houten&rft.aufirst=B&rft.date=2005-09-04&rft.volume=577&rft.issue=1-2&rft.spage=92&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.issn=00275107&rft_id=info:doi/10.1016%2Fj.mrfmmm.2005.03.013 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Molecular modelling; DNA damage; Nucleotide excision repair; Reviews; Nuclease; Mutagenesis DO - http://dx.doi.org/10.1016/j.mrfmmm.2005.03.013 ER - TY - JOUR T1 - Health risks and benefits of bis(4-chlorophenyl)-1,1,1-trichloroethane (DDT) AN - 17660363; 6499904 AB - DDT (bis[4-chlorophenyl]-1,1,1-trichloroethane) is a persistent insecticide that was used worldwide from the mid-1940s until its ban in the USA and other countries in the 1970s. When a global ban on DDT was proposed in 2001, several countries in sub-Saharan Africa claimed that DDT was still needed as a cheap and effective means for vector control. Although DDT is generally not toxic to human beings and was banned mainly for ecological reasons, subsequent research has shown that exposure to DDT at amounts that would be needed in malaria control might cause preterm birth and early weaning, abrogating the benefit of reducing infant mortality from malaria. Historically, DDT has had mixed success in Africa; only the countries that are able to find and devote substantial resources towards malaria control have made major advances. DDT might be useful in controlling malaria, but the evidence of its adverse effects on human health needs appropriate research on whether it achieves a favourable balance of risk versus benefit. JF - Lancet AU - Rogan, W J AU - Chen, A AD - Epidemiology Branch, US National Institute of Environmental Health Sciences, P O Box 12233, Research Triangle Park, NC 27709, USA, rogan@niehs.nih.gov Y1 - 2005/09/02/ PY - 2005 DA - 2005 Sep 02 SP - 763 EP - 773 VL - 366 IS - 9487 SN - 0099-5355, 0099-5355 KW - Risk Abstracts; Toxicology Abstracts; Health & Safety Science Abstracts KW - R2 23060:Medical and environmental health KW - H 12000:Epidemiology and Public Health KW - X 24136:Environmental impact UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17660363?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Thr105Ile%2C+a+functional+polymorphism+of+histamine+N-methyltransferase%2C+is+associated+with+alcoholism+in+two+independent+populations.&rft.au=Oroszi%2C+Gabor%3BEnoch%2C+Mary-Anne%3BChun%2C+Jeffrey%3BVirkkunen%2C+Matti%3BGoldman%2C+David&rft.aulast=Oroszi&rft.aufirst=Gabor&rft.date=2005-03-01&rft.volume=29&rft.issue=3&rft.spage=303&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Transcriptional Profiling Identifies Altered Intracellular Labile Iron Homeostasis as a Contributing Factor to the Toxicity of Adaphostin: Decreased Vascular Endothelial Growth Factor Secretion Is Independent of Hypoxia-Inducible Factor-1 Regulation AN - 745637228; 6529992 AB - PURPOSE: Adaphostin was developed as an inhibitor of the p210 super(bcr-abl) tyrosine kinase, but as its activity is not limited to tumor cell lines containing this translocation, transcriptional profiling was used as a tool to elucidate additional mechanisms responsible for adaphostin cytotoxicity. Experimental design: Profiles of drug-induced transcriptional changes were measured in three hematopoietic cell lines following 1 and 10 kmol/L adaphostin for 2 to 6 hours and then confirmed with real-time reverse transcription-PCR (2-24 hours). These data indicated altered iron homeostasis, and this was confirmed experimentally. Alteration of vascular endothelial growth factor (VEGF) secretion through hypoxia-inducible factor-1 (HIF-1) regulation was also investigated. RESULTS: Drug-induced genes included heat shock proteins and ubiquitins, but an intriguing response was the induction of ferritins. Measurement of the labile iron pool showed release of chelatable iron immediately after treatment with adaphostin and was quenched with the addition of an iron chelator. Pretreatment of cells with desferrioxamine and N-acetyl-cysteine reduced but did not ablate the sensitivity of the cells to adaphostin, and desferrioxamine was able to modulate adaphostin-induced activation of p38 and inactivation of AKT. VEGF secretion was shown to be reduced in cell lines after the addition of adaphostin but was not dependent on HIF-1. CONCLUSIONS: Adaphostin-induced cytotoxicity is caused in part by a rapid release of free iron, leading to redox perturbations and cell death. Despite this, reduced VEGF secretion was found to be independent of regulation by the redox responsive transcription factor HIF-1. Thus, adaphostin remains an interesting agent with the ability to kill tumor cells directly and modulate angiogenesis. JF - Clinical Cancer Research AU - Hose, Curtis AU - Kaur, Gurmeet AU - Sausville, Edward A AU - Monks, Anne AD - Authors' Affiliations: SAIC Frederick, Inc., Screening Technologies Branch, Laboratory of Functional Genomics, National Cancer Institute Frederick, Frederick, Maryland and Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Rockville, Maryland Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 6370 EP - 6381 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 17 SN - 1078-0432, 1078-0432 KW - Toxicology Abstracts KW - Vascular endothelial growth factor KW - Heat shock proteins KW - Data processing KW - Secretion KW - Angiogenesis KW - Transcription KW - Toxicity KW - Homeostasis KW - Chelating agents KW - Hypoxia-inducible factor 1 KW - Cytotoxicity KW - Tumor cell lines KW - Cell death KW - Transcription factors KW - Protein-tyrosine kinase KW - AKT protein KW - Hemopoiesis KW - Ferritin KW - Iron KW - Translocation KW - Deferoxamine KW - Ubiquitin KW - X 24360:Metals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/745637228?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Transcriptional+Profiling+Identifies+Altered+Intracellular+Labile+Iron+Homeostasis+as+a+Contributing+Factor+to+the+Toxicity+of+Adaphostin%3A+Decreased+Vascular+Endothelial+Growth+Factor+Secretion+Is+Independent+of+Hypoxia-Inducible+Factor-1+Regulation&rft.au=Hose%2C+Curtis%3BKaur%2C+Gurmeet%3BSausville%2C+Edward+A%3BMonks%2C+Anne&rft.aulast=Hose&rft.aufirst=Curtis&rft.date=2005-09-01&rft.volume=11&rft.issue=17&rft.spage=6370&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-07-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Vascular endothelial growth factor; Heat shock proteins; Data processing; Secretion; Angiogenesis; Transcription; Homeostasis; Toxicity; Chelating agents; Hypoxia-inducible factor 1; Cell death; Tumor cell lines; Cytotoxicity; Transcription factors; Protein-tyrosine kinase; AKT protein; Hemopoiesis; Ferritin; Translocation; Iron; Deferoxamine; Ubiquitin ER - TY - JOUR T1 - How to write an oncology manuscript. AN - 70175859; 16892095 AB - Publications may represent accomplishment in academic medicine, primary documentation of research data, evidence of expertise through writing an authoritative review paper or book chapter or a major determinant in achieving academic promotion and career development. Editors and reviewers appreciate receiving manuscripts that are easy to read and edit. Much of the information in journals instructions to authors is designed to accomplish that goal in ways that meet each journal's particular editorial needs. The CONsolidated Standards of Reporting Trial (CONSORT) statement is an important research tool that takes an evidence-based approach to improve the quality of reports of randomized trials. The guidance that follows provides a general background and rationale for preparing oncology manuscripts for any journal. Many of these guidelines are based on feedback provided by actual peer reviewers. Even before you start writing, it is good practice to review the typical sections of a manuscript The text of observational and experimental articles is usually (but not necessarily) divided into sections with the headings Introduction, Methods, Results, and Discussion. This so-called "IMRAD". The abstract of the manuscript is usually divided into background, purpose, patients and methods, results and conclusion. The section on patients and methods of an oncology manuscript should include the eligibility criteria for the patients, study design, treatment plan, baseline and treatment assessments and statistical analysis. The results include data on patient characteristics, tumor response, time to event measures, toxicity and dose administration. The conclusion must address the primary objective of the study. Authors will be able to address up front many issues regarding content, organization, presentation, and formatting, thereby increasing the likelihood of successful publication of their papers in peer-reviewed journals. JF - Journal of the Egyptian National Cancer Institute AU - Gaafar, Rabab AD - The Department of Medical Oncology, National Cancer Institute, Cairo University. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 132 EP - 138 VL - 17 IS - 3 SN - 1110-0362, 1110-0362 KW - Index Medicus KW - Humans KW - Writing -- standards KW - Publishing -- standards KW - Periodicals as Topic -- standards KW - Medical Oncology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70175859?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=Recovery+from+DSM-IV+alcohol+dependence%3A+United+States%2C+2001-2002.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BStinson%2C+Frederick+S%3BChou%2C+Patricia+S%3BHuang%2C+Boji%3BRuan%2C+W+June&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-03-01&rft.volume=100&rft.issue=3&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-14 N1 - Date created - 2006-08-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - [Mortality among workers in a cigarette factory in Lucca (Tuscany)]. TT - Studio di mortalità degli addetti alla manifattura del tabacco di Lucca. AN - 70160239; 16669164 AB - Aim of the present cohort study was to evaluate the mortality pattern among workers in a cigar and cigarette factory in Lucca, Italy. The study followed 2341 workers (1585 women and 756 men) registered in the company payrolls and employed for at least six months from 1 January 1960 through 1 January 1994. Follow-up was between the start of employment in the factory and 1 June 2002 (totally 74363,5 person-years). For both sexes, all-causes mortality was lower than expected (men: SMR= 0.8; CI95% 0.7-0.9; 158 deaths; women: SMR= 0.9; CI95% 0.8-1.0; 584 deaths) and no excess of mortality was reported for all malignant neoplasms. Among female workers, the frequency of deaths from pleural cancer was elevated at a statistically significant level (SMR= 6.0; CI95% 2.4-12.6; 5 deaths). One death for pleural cancer also occurred among men versus 0.4 expected. All women deceased from pleural cancer had been working in tobacco manufacturing for at least 30 years. The excess of pleural neoplasms reported in this study suggests the opportunity to evaluate the risk due to asbestos use in many manifacturing industries, especially where steam was used for extractive or warming purpose. JF - Epidemiologia e prevenzione AU - Parducci, Dino Ausilio AU - Puccetti, Monica AU - Bianchi Martini, Laura AU - Roselli, Maria Grazia AU - Vaghetti, Edoardo AU - Settimi, Laura AU - Orsi, Daniela AU - Battista, Giuseppe AD - UO Igiene e salute nei luoghi di lavoro, USL 2, Lucca. PY - 2005 SP - 271 EP - 277 VL - 29 IS - 5-6 SN - 1120-9763, 1120-9763 KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Sex Factors KW - Risk Factors KW - Humans KW - Cohort Studies KW - Adult KW - Middle Aged KW - Follow-Up Studies KW - Adolescent KW - Time Factors KW - Male KW - Italy KW - Female KW - Cause of Death KW - Pleural Neoplasms -- mortality KW - Mesothelioma -- etiology KW - Occupational Diseases -- etiology KW - Mesothelioma -- mortality KW - Occupational Exposure -- adverse effects KW - Asbestos -- adverse effects KW - Tobacco Industry KW - Pleural Neoplasms -- etiology KW - Occupational Diseases -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70160239?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiologia+e+prevenzione&rft.atitle=%5BMortality+among+workers+in+a+cigarette+factory+in+Lucca+%28Tuscany%29%5D.&rft.au=Parducci%2C+Dino+Ausilio%3BPuccetti%2C+Monica%3BBianchi+Martini%2C+Laura%3BRoselli%2C+Maria+Grazia%3BVaghetti%2C+Edoardo%3BSettimi%2C+Laura%3BOrsi%2C+Daniela%3BBattista%2C+Giuseppe&rft.aulast=Parducci&rft.aufirst=Dino&rft.date=2005-09-01&rft.volume=29&rft.issue=5-6&rft.spage=271&rft.isbn=&rft.btitle=&rft.title=Epidemiologia+e+prevenzione&rft.issn=11209763&rft_id=info:doi/ LA - Italian DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-31 N1 - Date created - 2006-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Metabolic effects of IGF-I deficiency: lessons from mouse models. AN - 68916750; 16369209 AB - Insulin and insulin-like growth factors (IGFs) belong to the most biologically characterized family of peptides involved in metabolism, growth and development. The cellular responses to the IGFs are mediated primarily by the IGF-I receptor. The IGF-I receptor is a member of the family of tyrosine kinase growth factor receptors, and is highly homologous (70%) to the insulin receptor, especially in the tyrosine kinase domain (84%) ADDIN. Upon ligand binding to the extracellular region, the intrinsic tyrosine kinase domain of the receptor is activated. In the past it was believed that insulin activates primarily metabolic processes while IGFs promote cell growth and differentiation. However, in the last two decades many animal models of IGFI deficiency and excess revealed the importance of IGF-I in carbohydrate and lipid metabolism and now it is clear that these peptide hormones together with growth hormone (GH) work in a coordinate and interdependent manner. In the circulation, IGFs are bound in a binary complex with a family of high affinity IGF-binding proteins (IGFBPs) ADDIN. However, most of the circulating IGF-I associates with a high molecular weight complex approximately 150 KDa consisting of IGFBP-3 and the acid labile subunit (ALS) ADDIN. Once the ternary complex dissociates, the binary complexes of IGFBP-IGFs are removed from the circulation and by crossing the endothelium to reach the target tissues and to interact with cell surface receptors. In the present review we will summarize the role of GH and IGF in somatic growth and focus on the metabolic effects of IGF-I deficiency as assessed in various mouse models. JF - Pediatric endocrinology reviews : PER AU - Yakar, Shoshana AU - Sun, Hui AU - Zhao, Hong AU - Pennisi, Patricia AU - Toyoshima, Yuka AU - Setser, Jennifer AU - Stannard, Bethel AU - Scavo, Louis AU - Leroith, Derek AD - Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. ShoshanaY@intra.niddk.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 11 EP - 19 VL - 3 IS - 1 SN - 1565-4753, 1565-4753 KW - Insulin KW - 0 KW - Human Growth Hormone KW - 12629-01-5 KW - Insulin-Like Growth Factor I KW - 67763-96-6 KW - Index Medicus KW - Animals KW - Drug Interactions KW - Liver -- metabolism KW - Mice KW - Insulin -- pharmacology KW - Mice, Transgenic KW - Metabolism KW - Mice, Knockout KW - Human Growth Hormone -- physiology KW - Human Growth Hormone -- pharmacology KW - Insulin-Like Growth Factor I -- genetics KW - Insulin-Like Growth Factor I -- physiology KW - Insulin-Like Growth Factor I -- deficiency KW - Disease Models, Animal UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68916750?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatric+endocrinology+reviews+%3A+PER&rft.atitle=Metabolic+effects+of+IGF-I+deficiency%3A+lessons+from+mouse+models.&rft.au=Yakar%2C+Shoshana%3BSun%2C+Hui%3BZhao%2C+Hong%3BPennisi%2C+Patricia%3BToyoshima%2C+Yuka%3BSetser%2C+Jennifer%3BStannard%2C+Bethel%3BScavo%2C+Louis%3BLeroith%2C+Derek&rft.aulast=Yakar&rft.aufirst=Shoshana&rft.date=2005-09-01&rft.volume=3&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Pediatric+endocrinology+reviews+%3A+PER&rft.issn=15654753&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-12 N1 - Date created - 2005-12-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The effects of the going places program on early adolescent substance use and antisocial behavior. AN - 68907519; 16091854 AB - This study evaluated the effects of a school-based intervention on growth trajectories of smoking, drinking, and antisocial behavior among early adolescents. Seven middle schools were randomized to intervention or comparison conditions and students in two successive cohorts (n = 1484) provided five waves of data from sixth to ninth grade. The Going Places Program, included classroom curricula, parent education, and school environment components. Latent growth curve analyses demonstrated significant treatment group effects, including reducing increases in friends who smoke, outcome expectations for smoking, and smoking progression, but had non-significant effects on drinking or antisocial behavior. The Going Places Program was effective in preventing increases in smoking progression, but its efficacy as a more cross-cutting problem behavior preventive intervention was not confirmed. JF - Prevention science : the official journal of the Society for Prevention Research AU - Simons-Morton, Bruce AU - Haynie, Denise AU - Saylor, Keith AU - Crump, Aria Davis AU - Chen, Rusan AD - Prevention Research Branch DESPR, NICHD, NIH, Rockville, Maryland 20852-7510, USA. Mortonb@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 187 EP - 197 VL - 6 IS - 3 SN - 1389-4986, 1389-4986 KW - Index Medicus KW - Humans KW - Curriculum KW - Maryland KW - Adolescent KW - Male KW - Female KW - Schools KW - Antisocial Personality Disorder -- prevention & control KW - Substance-Related Disorders -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68907519?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prevention+science+%3A+the+official+journal+of+the+Society+for+Prevention+Research&rft.atitle=The+effects+of+the+going+places+program+on+early+adolescent+substance+use+and+antisocial+behavior.&rft.au=Simons-Morton%2C+Bruce%3BHaynie%2C+Denise%3BSaylor%2C+Keith%3BCrump%2C+Aria+Davis%3BChen%2C+Rusan&rft.aulast=Simons-Morton&rft.aufirst=Bruce&rft.date=2005-09-01&rft.volume=6&rft.issue=3&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Prevention+science+%3A+the+official+journal+of+the+Society+for+Prevention+Research&rft.issn=13894986&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-14 N1 - Date created - 2005-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Enhancement of hidden structures of early skin fibrosis using polarization degree patterns and Pearson correlation analysis. AN - 68811346; 16292958 AB - The skin of athymic nude mice is irradiated with a single dose of x-ray irradiation that initiated fibrosis. Digital photographs of the irradiated mice are taken by illuminating the mouse skin with linearly polarized probe light of 650 nm. The specific pattern of the surface distribution of the degree of polarization enables the detection of initial skin fibrosis structures that were not visually apparent. Data processing of the raw spatial distributions of the degree of polarization based on Fourier filtering of the high-frequency noise improves subjective perception of the revealed structure in the images. In addition, Pearson correlation analysis provides information about skin structural size and directionality. JF - Journal of biomedical optics AU - Sviridov, Alexander P AU - Chernomordik, Victor AU - Hassan, Moinuddin AU - Boccara, Albert C AU - Russo, Angelo AU - Smith, Paul AU - Gandjbakhche, Amir AD - National Institutes of Health, Bethesda, Maryland 20892, USA. PY - 2005 SP - 051706 VL - 10 IS - 5 SN - 1083-3668, 1083-3668 KW - Index Medicus KW - Sensitivity and Specificity KW - Animals KW - X-Rays KW - Reproducibility of Results KW - Fibrosis KW - Mice, Nude KW - Mice KW - Statistics as Topic KW - Fourier Analysis KW - Image Interpretation, Computer-Assisted -- methods KW - Signal Processing, Computer-Assisted KW - Radiodermatitis -- pathology KW - Algorithms KW - Image Enhancement -- methods KW - Microscopy, Polarization -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68811346?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+biomedical+optics&rft.atitle=Enhancement+of+hidden+structures+of+early+skin+fibrosis+using+polarization+degree+patterns+and+Pearson+correlation+analysis.&rft.au=Sviridov%2C+Alexander+P%3BChernomordik%2C+Victor%3BHassan%2C+Moinuddin%3BBoccara%2C+Albert+C%3BRusso%2C+Angelo%3BSmith%2C+Paul%3BGandjbakhche%2C+Amir&rft.aulast=Sviridov&rft.aufirst=Alexander&rft.date=2005-09-01&rft.volume=10&rft.issue=5&rft.spage=051706&rft.isbn=&rft.btitle=&rft.title=Journal+of+biomedical+optics&rft.issn=10833668&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-04-04 N1 - Date created - 2005-11-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Overexpression of 15-lipoxygenase-1 induces growth arrest through phosphorylation of p53 in human colorectal cancer cells. AN - 68616244; 16179498 AB - To investigate the function of 15-lipoxygenase-1 (15-LOX-1) in human colorectal cancer, we overexpressed 15-LOX-1 in HCT-116 human colorectal cancer cells. Clones expressing the highest levels of 15-LOX-1 displayed reduced viability compared with the HCT-116-Vector control cells. Further, by cell cycle gene array analyses, the cyclin-dependent kinase inhibitor p21WAF1/CIP1 and MDM2 genes were up-regulated in 15-LOX-1-overexpressing cells. The induction of p21(WAF1/CIP1) and MDM2 were linked to activation of p53 by 15-LOX-1, as there was a dramatic induction of phosphorylated p53 (Ser15) in 15-LOX-1-overesxpressing cells. However, the 15-LOX-1 metabolites 13(S)-hydroxyoctadecadienoic acid and 15(S)-hydroxyeicosatetraenoic acid failed to induce phosphorylation of p53 at Ser15, and the 15-LOX-1 inhibitor PD146176 did not inhibit the phosphorylation of p53 at Ser15 in 15-LOX-1-overexpressing cells. Nonetheless, the growth-inhibitory effects of 15-LOX-1 were p53 dependent, as 15-LOX-1 overexpression had no effect on cell growth in p53 (-/-) HCT-116 cells. Finally, treatment of HCT-116-15-LOX-1 cells with different kinase inhibitors suggested that the effects of 15-LOX-1 on p53 phosphorylation and activation were due to effects on DNA-dependent protein kinase. Collectively, these findings suggest a new mechanism to explain the biological activity of 15-LOX-1, where 15-LOX plays a stoichiometric role in activating a DNA-dependent protein kinase-dependent pathway that leads to p53-dependent growth arrest. JF - Molecular cancer research : MCR AU - Kim, Jong-Sik AU - Baek, Seung Joon AU - Bottone, Frank G AU - Sali, Tina AU - Eling, Thomas E AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, MD, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 511 EP - 517 VL - 3 IS - 9 SN - 1541-7786, 1541-7786 KW - CDKN1A protein, human KW - 0 KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - DNA-Binding Proteins KW - Linoleic Acids KW - Lipoxygenase Inhibitors KW - Nuclear Proteins KW - Proto-Oncogene Proteins KW - Tumor Suppressor Protein p53 KW - 13-hydroxy-9,11-octadecadienoic acid KW - 5204-88-6 KW - 15-hydroxy-5,8,11,13,17-eicosapentaenoic acid KW - 97850-14-1 KW - Eicosapentaenoic Acid KW - AAN7QOV9EA KW - Arachidonate 15-Lipoxygenase KW - EC 1.13.11.33 KW - MDM2 protein, human KW - EC 2.3.2.27 KW - Proto-Oncogene Proteins c-mdm2 KW - DNA-Activated Protein Kinase KW - EC 2.7.11.1 KW - PRKDC protein, human KW - Protein-Serine-Threonine Kinases KW - Index Medicus KW - Humans KW - DNA-Binding Proteins -- pharmacology KW - Proto-Oncogene Proteins -- metabolism KW - Eicosapentaenoic Acid -- analogs & derivatives KW - HCT116 Cells KW - Cell Cycle Proteins -- metabolism KW - Gene Expression Regulation, Neoplastic KW - Protein-Serine-Threonine Kinases -- pharmacology KW - Tumor Cells, Cultured KW - Phosphorylation KW - Lipoxygenase Inhibitors -- pharmacology KW - Linoleic Acids -- metabolism KW - Nuclear Proteins -- metabolism KW - Eicosapentaenoic Acid -- metabolism KW - Cell Division KW - Arachidonate 15-Lipoxygenase -- metabolism KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68616244?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+research+%3A+MCR&rft.atitle=Overexpression+of+15-lipoxygenase-1+induces+growth+arrest+through+phosphorylation+of+p53+in+human+colorectal+cancer+cells.&rft.au=Kim%2C+Jong-Sik%3BBaek%2C+Seung+Joon%3BBottone%2C+Frank+G%3BSali%2C+Tina%3BEling%2C+Thomas+E&rft.aulast=Kim&rft.aufirst=Jong-Sik&rft.date=2005-09-01&rft.volume=3&rft.issue=9&rft.spage=511&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+research+%3A+MCR&rft.issn=15417786&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-10 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In islet-specific glucose-6-phosphatase-related protein, the beta cell antigenic sequence that is targeted in diabetes is not responsible for the loss of phosphohydrolase activity. AN - 68586139; 16012821 AB - There are three members of the glucose-6-phosphatase (G6Pase) family: (1) the liver/kidney/intestine G6Pase-alpha (encoded by G6PC), which is a key enzyme in glucose homeostasis; (2) the ubiquitous G6Pase-beta (encoded by G6PC3); and (3) the islet-specific G6Pase-related protein (IGRP, encoded by /G6PC2). While G6Pase-alpha and G6Pase-beta are functional glucose-6-phosphate hydrolases, IGRP possesses almost no hydrolase activity. This was unexpected since G6Pase-alpha is more closely related to IGRP than G6Pase-beta. Recently, amino acids 206-214 in IGRP were identified as a beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes, suggesting that this peptide confers functional specificity to IGRP. We therefore investigated the molecular events that inactivate IGRP activity and the effects of the beta cell antigen sequence on the stability and enzymatic activity of G6Pase-alpha. Studies were performed using site-directed mutagenesis and transient expression assays. Protein stability was evaluated by Western blotting, proteasome inhibitor studies and in vitro transcription-translation. We showed that the residues responsible for G6Pase activity are more extensive than previously recognised. Introducing the IGRP antigenic motif into G6Pase-alpha does not completely destroy activity, although it does destabilise the protein. The low hydrolytic activity in IGRP is due to the combination of multiple independent mutations. The loss of catalytic activity in IGRP arises from the sum of many sequence differences. G6Pase-alpha mutants containing the beta cell antigen sequence are preferentially degraded in cells, which prevents targeting by pathogenic CD8+ T cells. It is possible that IGRP levels in beta cells could dictate susceptibilities to diabetes. JF - Diabetologia AU - Shieh, J-J AU - Pan, C-J AU - Mansfield, B C AU - Chou, J Y AD - Section on Cellular Differentiation, Heritable Disorders Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1830, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1851 EP - 1859 VL - 48 IS - 9 SN - 0012-186X, 0012-186X KW - DNA Primers KW - 0 KW - Phosphoric Monoester Hydrolases KW - EC 3.1.3.2 KW - Glucose-6-Phosphatase KW - EC 3.1.3.9 KW - G6PC2 protein, human KW - EC 3.1.3.9. KW - Index Medicus KW - Animals KW - COS Cells KW - Humans KW - Amino Acid Sequence KW - Mice KW - Cloning, Molecular KW - Rats KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Sequence Alignment KW - Conserved Sequence KW - Cercopithecus aethiops KW - Molecular Sequence Data KW - Dogs KW - Sequence Homology, Amino Acid KW - Amino Acid Substitution KW - Glucose-6-Phosphatase -- chemistry KW - Glucose-6-Phosphatase -- physiology KW - Islets of Langerhans -- physiology KW - Islets of Langerhans -- enzymology KW - Phosphoric Monoester Hydrolases -- metabolism KW - Glucose-6-Phosphatase -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68586139?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diabetologia&rft.atitle=In+islet-specific+glucose-6-phosphatase-related+protein%2C+the+beta+cell+antigenic+sequence+that+is+targeted+in+diabetes+is+not+responsible+for+the+loss+of+phosphohydrolase+activity.&rft.au=Shieh%2C+J-J%3BPan%2C+C-J%3BMansfield%2C+B+C%3BChou%2C+J+Y&rft.aulast=Shieh&rft.aufirst=J-J&rft.date=2005-09-01&rft.volume=48&rft.issue=9&rft.spage=1851&rft.isbn=&rft.btitle=&rft.title=Diabetologia&rft.issn=0012186X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-03 N1 - Date created - 2005-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevention of alcohol-induced developmental delays and learning abnormalities in a model of fetal alcohol syndrome. AN - 68581133; 16157106 AB - Prenatal alcohol exposure results in fetal death and neurobehavioral complications including learning impairment. Previously synthetic peptides derived from activity-dependent neurotrophic factor have been shown to prevent aspects of alcohol-induced damage in pregnancy. The objective of this work was to evaluate whether activity-dependent neurotrophic factor-12 could prevent alcohol-induced damage in a model of fetal alcohol syndrome. Using a well-characterized model, C57Bl6/J mice on gestational day 8 were treated with placebo, alcohol (30% volume/volume alcohol 0.03 mL/kg), alcohol plus activity-dependent neurotrophic factor-12 30 minutes prior to alcohol, or activity-dependent neurotrophic factor-12 alone. Fetal death was assessed on gestational day 18 (25 litters were evaluated: alcohol, n = 5; placebo, n = 9; alcohol plus activity-dependent neurotrophic factor-12, n = 11). Neonatal behavior tests were performed on postnatal days 1 through 21 with the offspring of 12 dams (alcohol, n = 16; placebo, n = 46; alcohol plus activity-dependent neurotrophic factor-12, n = 23; and activity-dependent neurotrophic factor-12, n = 35). Adult males were tested in the Morris water maze for learning assessment and with the hole punch activity test for exploratory activity. Statistical analysis included Kruskal-Wallis and analysis of variance. Fetal death was greater in alcohol (67% +/- 13%) vs placebo (8.4% +/- 3%, P .5) and significantly better than alcohol on days 4, 6, and 7 (all P < .05). Alcohol exposure resulted in significantly less time in hole punch activity (P < .02) than control. Activity-dependent neurotrophic factor-12 pretreatment prevented the alcohol-induced decline, with levels the same as control (P = .1). The novel peptide activity-dependent neurotrophic factor-12 prevents alcohol-induced fetal death and developmental and learning abnormalities in a model of fetal alcohol syndrome. This demonstrates that a single treatment with a peptide is efficacious and may be of value in the prevention of alcohol-induced damage. JF - American journal of obstetrics and gynecology AU - Endres, M AU - Toso, L AU - Roberson, R AU - Park, J AU - Abebe, D AU - Poggi, S AU - Spong, C Y AD - Unit on Perinatal and Developmental Neurobiology, National Insitute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-0925, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1028 EP - 1034 VL - 193 IS - 3 Pt 2 SN - 0002-9378, 0002-9378 KW - Nerve Tissue Proteins KW - 0 KW - Ethanol KW - 3K9958V90M KW - Abridged Index Medicus KW - Index Medicus KW - Mice, Inbred Strains KW - Fetal Death -- prevention & control KW - Animals KW - Cognition -- drug effects KW - Motor Activity KW - Learning -- drug effects KW - Disease Models, Animal KW - Mice KW - Fetal Growth Retardation -- prevention & control KW - Female KW - Pregnancy KW - Maze Learning KW - Fetal Alcohol Spectrum Disorders -- physiopathology KW - Ethanol -- administration & dosage KW - Nerve Tissue Proteins -- therapeutic use KW - Learning Disorders -- prevention & control KW - Learning Disorders -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68581133?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+obstetrics+and+gynecology&rft.atitle=Prevention+of+alcohol-induced+developmental+delays+and+learning+abnormalities+in+a+model+of+fetal+alcohol+syndrome.&rft.au=Endres%2C+M%3BToso%2C+L%3BRoberson%2C+R%3BPark%2C+J%3BAbebe%2C+D%3BPoggi%2C+S%3BSpong%2C+C+Y&rft.aulast=Endres&rft.aufirst=M&rft.date=2005-09-01&rft.volume=193&rft.issue=3+Pt+2&rft.spage=1028&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=00029378&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-09-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Interdisciplinary neurotoxicity inhalation studies: carbon disulfide and carbonyl sulfide research in F344 rats. AN - 68576298; 16002115 AB - Inhalation studies were conducted on the hazardous air pollutants, carbon disulfide, which targets the central nervous system (spinal cord) and peripheral nervous system (distal portions of long myelinated axons), and carbonyl sulfide, which targets the central nervous system (brain). The objectives were to investigate the neurotoxicity of these compounds by a comprehensive evaluation of function, structure, and mechanisms of disease. Through interdisciplinary research, the major finding in the carbon disulfide inhalation studies was that carbon disulfide produced intra- and intermolecular protein cross-linking in vivo. The observation of dose-dependent covalent cross-linking in neurofilament proteins prior to the onset of lesions is consistent with this process contributing to the development of the neurofilamentous axonal swellings characteristic of carbon disulfide neurotoxicity. Of significance is that valine-lysine thiourea cross-linking on rat globin and lysine-lysine thiourea cross-linking on erythrocyte spectrin reflect cross-linking events occurring within the axon and could potentially serve as biomarkers of carbon disulfide exposure and effect. In the carbonyl sulfide studies, using magnetic resonance microscopy (MRM), we determined that carbonyl sulfide targets the auditory pathway in the brain. MRM allowed the examination of 200 brain slices and made it possible to identify the most vulnerable sites of neurotoxicity, which would have been missed in our traditional neuropathology evaluations. Electrophysiological studies were focused on the auditory system and demonstrated decreases in auditory brain stem evoked responses. Similarly, mechanistic studies focused on evaluating cytochrome oxidase activity in the posterior colliculus and parietal cortex. A decrease in cytochrome oxidase activity was considered to be a contributing factor to the pathogenesis of carbonyl sulfide neurotoxicity. JF - Toxicology and applied pharmacology AU - Sills, Robert C AU - Harry, G Jean AU - Valentine, William M AU - Morgan, Daniel L AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, 111 Alexander Drive, South Campus, MD B3-08, PO Box 12233, Research Triangle Park, NC 27709, USA. sills@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 245 EP - 250 VL - 207 IS - 2 Suppl KW - Sulfur Oxides KW - 0 KW - carbonyl sulfide KW - 871UI0ET21 KW - Carbon Disulfide KW - S54S8B99E8 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Rats, Inbred F344 KW - Administration, Inhalation KW - Carbon Disulfide -- toxicity KW - Nervous System -- drug effects KW - Sulfur Oxides -- administration & dosage KW - Sulfur Oxides -- toxicity KW - Carbon Disulfide -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68576298?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Interdisciplinary+neurotoxicity+inhalation+studies%3A+carbon+disulfide+and+carbonyl+sulfide+research+in+F344+rats.&rft.au=Sills%2C+Robert+C%3BHarry%2C+G+Jean%3BValentine%2C+William+M%3BMorgan%2C+Daniel+L&rft.aulast=Sills&rft.aufirst=Robert&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=245&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Complementary gene and protein expression studies and integrative approaches in toxicogenomics. AN - 68576161; 15992845 AB - Parallel transcript and protein profiling is a strategy to gain further insight into the mechanisms of toxicity and disease. The technologies used to measure expression at the transcript and protein levels each convey different information and have different technical capabilities that can complement each other when combined. In this review, over twenty studies are considered for the use of -Omics platform, the chemical or disease being profiled, tissues, the number of genes and proteins found by each platform and common expression products. A strategy is suggested for toxicant expression profiling that combines the transcriptomics and proteomics of both the target tissue and blood/serum that could provide a more complete characterization of toxicity as well as synergize expression technologies toward biomarker discovery. JF - Toxicology and applied pharmacology AU - Merrick, B Alex AU - Madenspacher, Jennifer H AD - National Center for Toxicogenomics, National Institute of Environmental Health Sciences, D2-04, P.O. Box 12233, Research Triangle Park, NC 27709, USA. merrick@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 189 EP - 194 VL - 207 IS - 2 Suppl KW - Index Medicus KW - Animals KW - Humans KW - Gene Expression Profiling KW - Proteomics KW - Toxicology KW - Genomics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68576161?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Complementary+gene+and+protein+expression+studies+and+integrative+approaches+in+toxicogenomics.&rft.au=Merrick%2C+B+Alex%3BMadenspacher%2C+Jennifer+H&rft.aulast=Merrick&rft.aufirst=B&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=189&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene-particulate matter-health interactions. AN - 68575289; 15979667 AB - Inter-individual variation in human responses to air pollutants suggests that some subpopulations are at increased risk to the detrimental effects of pollutant exposure. Extrinsic factors such as previous exposure and nutritional status may influence individual susceptibility. Intrinsic (host) factors that determine susceptibility include age, gender, and pre-existing disease (e.g., asthma), and it is becoming clear that genetic background also contributes to individual susceptibility. Environmental exposures to particulates and genetic factors associated with disease risk likely interact in a complex fashion that varies from one population and one individual to another. The relationships between genetic background and disease risk and severity are often evaluated through traditional family-based linkage studies and positional cloning techniques. However, case-control studies based on association of disease or disease subphenotypes with candidate genes have advantages over family pedigree studies for complex disease phenotypes. This is based in part on continued development of quantitative analysis and the discovery and availability of simple sequence repeats and single nucleotide polymorphisms. Linkage analyses with genetically standardized animal models also provide a useful tool to identify genetic determinants of responses to environmental pollutants. These approaches have identified significant susceptibility quantitative trait loci on mouse chromosomes 1, 6, 11, and 17. Physical mapping and comparative mapping between human and mouse genomes will yield candidate susceptibility genes that may be tested by association studies in human subjects. Human studies and mouse modeling will provide important insight to understanding genetic factors that contribute to differential susceptibility to air pollutants. JF - Toxicology and applied pharmacology AU - Kleeberger, Steven R AU - Ohtsuka, Yoshinori AD - Laboratory of Respiratory Biology, Environmental Genetics Group, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Drive, Research Triangle Park, NC 27705, USA. kleeber1@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 276 EP - 281 VL - 207 IS - 2 Suppl KW - Air Pollutants KW - 0 KW - Index Medicus KW - Genetic Linkage KW - Animals KW - Polymorphism, Single Nucleotide KW - Particle Size KW - Humans KW - Case-Control Studies KW - Repetitive Sequences, Nucleic Acid KW - Air Pollutants -- toxicity KW - Genetics, Medical UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68575289?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Gene-particulate+matter-health+interactions.&rft.au=Kleeberger%2C+Steven+R%3BOhtsuka%2C+Yoshinori&rft.aulast=Kleeberger&rft.aufirst=Steven&rft.date=2005-09-01&rft.volume=207&rft.issue=2+Suppl&rft.spage=276&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=1096-0333&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-10-01 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dustborne Alternaria alternata antigens in US homes: results from the National Survey of Lead and Allergens in Housing. AN - 68573555; 16159634 AB - Alternaria alternata is one of the most common fungi associated with allergic disease. However, Alternaria exposure in indoor environments is not well characterized. The primary goals of this study were to examine the prevalence of Alternaria exposure and identify independent predictors of Alternaria antigen concentrations in US homes. Data for this cross-sectional study were obtained from the National Survey of Lead and Allergens in Housing. A nationally representative sample of 831 housing units in 75 different locations throughout the United States completed the survey. Information on housing and household characteristics was obtained by questionnaire and environmental assessments. Concentrations of A alternata antigens in dust collected from various indoor sites were assessed with a polyclonal anti-Alternaria antibody assay. Alternaria antigens were detected in most (95% to 99%) of the dust samples. The geometric mean concentration, reflecting the average Alternaria concentration in homes, was 4.88 microg/g (SEM, 0.13 microg/g). In the multivariable linear regression analysis, the age of the housing unit, geographic region, urbanization, poverty, family race, observed mold and moisture problems, use of de-humidifier, and presence of cats and dogs were independent predictors of Alternaria antigen concentrations. Less frequent cleaning and smoking indoors also contributed to higher Alternaria antigen levels in homes. Exposure to A alternata antigens in US homes is common. Antigen levels in homes are influenced not only by regional factors but also by residential characteristics. Preventing mold and moisture problems, avoiding smoking indoors, and regular household cleaning may help reduce exposure to Alternaria antigens indoors. JF - The Journal of allergy and clinical immunology AU - Salo, Päivi M AU - Yin, Ming AU - Arbes, Samuel J AU - Cohn, Richard D AU - Sever, Michelle AU - Muilenberg, Michael AU - Burge, Harriet A AU - London, Stephanie J AU - Zeldin, Darryl C AD - National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 623 EP - 629 VL - 116 IS - 3 SN - 0091-6749, 0091-6749 KW - Antigens, Fungal KW - 0 KW - Dust KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Cross-Sectional Studies KW - Animals KW - Housing KW - Humans KW - Humidity KW - Prevalence KW - Antigens, Fungal -- immunology KW - Air Pollution, Indoor KW - Dust -- immunology KW - Environmental Exposure KW - Alternaria -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68573555?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+allergy+and+clinical+immunology&rft.atitle=Dustborne+Alternaria+alternata+antigens+in+US+homes%3A+results+from+the+National+Survey+of+Lead+and+Allergens+in+Housing.&rft.au=Salo%2C+P%C3%A4ivi+M%3BYin%2C+Ming%3BArbes%2C+Samuel+J%3BCohn%2C+Richard+D%3BSever%2C+Michelle%3BMuilenberg%2C+Michael%3BBurge%2C+Harriet+A%3BLondon%2C+Stephanie+J%3BZeldin%2C+Darryl+C&rft.aulast=Salo&rft.aufirst=P%C3%A4ivi&rft.date=2005-09-01&rft.volume=116&rft.issue=3&rft.spage=623&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+allergy+and+clinical+immunology&rft.issn=00916749&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-15 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Allergy Clin Immunol. 2003 Feb;111(2):285-9 [12589346] Pediatr Allergy Immunol. 2003 Apr;14(2):100-5 [12675755] J Allergy Clin Immunol. 2004 Feb;113(2):227-34 [14767434] J Allergy Clin Immunol. 2004 Sep;114(3):599-606 [15356564] Ann Allergy. 1981 Jan;46(1):30-3 [7192959] J Allergy Clin Immunol. 1985 Dec;76(6):819-25 [4067131] Ann Allergy. 1990 Aug;65(2):109-14 [2382872] N Engl J Med. 1991 Feb 7;324(6):359-63 [1987459] J Allergy Clin Immunol. 1992 Mar;89(3):752-9 [1545096] J Allergy Clin Immunol. 1993 Mar;91(3):773-82 [8454800] Clin Microbiol Rev. 1995 Apr;8(2):161-79 [7621398] Am J Respir Crit Care Med. 1997 Apr;155(4):1356-61 [9105079] Clin Exp Allergy. 1998 Apr;28(4):459-67 [9641573] J Allergy Clin Immunol. 1998 Oct;102(4 Pt 1):563-70 [9802363] J Allergy Clin Immunol. 1999 Mar;103(3 Pt 1):494-500 [10069885] J Allergy Clin Immunol. 1999 Apr;103(4):709-11 [10200024] J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):665-71 [10482844] Can J Public Health. 1999 Jul-Aug;90(4):244-9 [10489721] Clin Exp Allergy. 1999 Nov;29(11):1481-9 [10520075] Acta Allergol. 1962;17:130-59 [14492419] J Expo Anal Environ Epidemiol. 1999 Nov-Dec;9(6):560-8 [10638841] Ann Allergy Asthma Immunol. 2000 Jan;84(1):47-54 [10674565] Environ Health Perspect. 2000 Aug;108 Suppl 4:653-9 [10931783] Clin Exp Allergy. 2000 Dec;30(12):1733-9 [11122211] J Allergy Clin Immunol. 2001 Feb;107(2):388-90 [11174210] J Allergy Clin Immunol. 2001 Mar;107(3 Suppl):S430-40 [11242604] J Allergy Clin Immunol. 2001 Apr;107(4):641-6 [11295652] Ann Allergy Asthma Immunol. 2001 May;86(5):517-23 [11379802] Am J Respir Crit Care Med. 2001 Aug 1;164(3):455-9 [11500349] Appl Environ Microbiol. 2002 Apr;68(4):1743-53 [11916692] Environ Health Perspect. 2002 May;110(5):527-32 [12003758] BMJ. 2002 Aug 24;325(7361):411-4 [12193354] J Expo Anal Environ Epidemiol. 2002 Nov;12(6):427-32 [12415491] Allergy. 2003 Jan;58(1):13-20 [12580801] Environ Health Perspect. 1997 Jun;105(6):622-35 [9288497] Mycopathologia. 1997;139(1):23-33 [9511234] Ann Allergy Asthma Immunol. 1998 Mar;80(3):279-85 [9532979] J Allergy Clin Immunol. 1998 May;101(5):626-32 [9600499] Comment In: J Allergy Clin Immunol. 2005 Sep;116(3):620-2 [16159633] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Novel peptides prevent alcohol-induced spatial learning deficits and proinflammatory cytokine release in a mouse model of fetal alcohol syndrome. AN - 68567818; 16150281 AB - Previously, the novel peptides NAPVSIPQ and SALLRSIPA were shown to prevent alcohol-induced fetal death and growth abnormalities in a mouse model of fetal alcohol syndrome. This study evaluated whether these peptides could prevent long-term alcohol-induced learning abnormalities. In addition, because specific cytokines are known to effect long-term potentiation, a model of learning at the molecular level, we studied the effect of these novel peptides on tumor necrosis factor-alpha, interleukin-6, and interferon-gamma levels. We used a well-characterized mouse model of fetal alcohol syndrome. Pregnant mice were injected on day 8 with alcohol (0.03 mL/kg) or placebo. Pretreatment with NAPVSIPQ+SALLRSIPA (20 mug) or placebo was given 30 minutes before alcohol. Embryos were removed after 6 hours, at which time cytokine, tumor necrosis factor-alpha, interleukin-6, and interferon-gamma levels were measured with enzyme-linked immunoassays. To test spatial learning, adult offspring from litters that were treated with alcohol, control, NAPVSIPQ+SALLRSIPA then alcohol, or NAPVSIPQ+SALLRSIPA alone were evaluated for latency to find a hidden platform in the Morris water maze. Alcohol treatment increased tumor necrosis factor-alpha levels versus control levels (50.0 +/- 3.5 pg/mL vs 32.7 +/- 2.4 pg/mL; P < .001). NAPVSIPQ+SALLRSIPA pretreatment prevented this increase (39.9 9 +/- 2.8 pg/mL; P or = 60% with no prior history of autoimmunity. Twenty-nine patients received 3 mg/kg anti-CTLA-4 every 3 weeks, whereas 27 received 3 mg/kg as their initial dose with subsequent doses reduced to 1 mg/kg every 3 weeks. In both cohorts patients received concomitant vaccination with two modified HLA-A*0201-restricted peptides from the gp100 melanoma-associated antigen, gp100:209-217(210M) and gp100:280-288(288V). Two patients achieved a complete response (ongoing at 30 and 31 months, respectively) and five patients achieved a partial response (durations of 4, 6, 25+, 26+, and 34+ months, respectively), for an overall objective response rate of 13%. Tumor regression was seen in lung, liver, brain, lymph nodes, and subcutaneous sites. Of 14 patients with grade 3/4 autoimmune toxicity, five (36%) experienced a clinical response compared with only two responses in the 42 patients (5%) with no autoimmune toxicity (P = .008). There were no significant differences in response rate or toxicity between the two dose schedules. Administration of anti-CTLA-4 monoclonal antibody plus peptide vaccination can cause durable objective responses, which correlate with the induction of autoimmunity, in patients with metastatic melanoma. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Attia, Peter AU - Phan, Giao Q AU - Maker, Ajay V AU - Robinson, Michael R AU - Quezado, Martha M AU - Yang, James C AU - Sherry, Richard M AU - Topalian, Suzanne L AU - Kammula, Udai S AU - Royal, Richard E AU - Restifo, Nicholas P AU - Haworth, Leah R AU - Levy, Catherine AU - Mavroukakis, Sharon A AU - Nichol, Geoff AU - Yellin, Michael J AU - Rosenberg, Steven A AD - Surgery Branch, National Cancer Institute, National Institutes of Health, CRC, Room 3W-3940, 10 Center Dr, Bethesda, MD 20892-1201, USA. Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 6043 EP - 6053 VL - 23 IS - 25 SN - 0732-183X, 0732-183X KW - Antigens, CD KW - 0 KW - Antigens, Differentiation KW - CTLA-4 Antigen KW - CTLA4 protein, human KW - Cancer Vaccines KW - Immunosuppressive Agents KW - Index Medicus KW - Drug Administration Schedule KW - Injections, Intravenous KW - Humans KW - Adult KW - Treatment Outcome KW - Prognosis KW - Aged KW - Middle Aged KW - Male KW - Female KW - Skin Neoplasms -- drug therapy KW - Cancer Vaccines -- immunology KW - Skin Neoplasms -- immunology KW - Antigens, Differentiation -- immunology KW - Melanoma -- drug therapy KW - Cancer Vaccines -- therapeutic use KW - Immunosuppressive Agents -- immunology KW - Autoimmunity KW - Antigens, Differentiation -- administration & dosage KW - Melanoma -- immunology KW - Immunosuppressive Agents -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68544804?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Autoimmunity+correlates+with+tumor+regression+in+patients+with+metastatic+melanoma+treated+with+anti-cytotoxic+T-lymphocyte+antigen-4.&rft.au=Attia%2C+Peter%3BPhan%2C+Giao+Q%3BMaker%2C+Ajay+V%3BRobinson%2C+Michael+R%3BQuezado%2C+Martha+M%3BYang%2C+James+C%3BSherry%2C+Richard+M%3BTopalian%2C+Suzanne+L%3BKammula%2C+Udai+S%3BRoyal%2C+Richard+E%3BRestifo%2C+Nicholas+P%3BHaworth%2C+Leah+R%3BLevy%2C+Catherine%3BMavroukakis%2C+Sharon+A%3BNichol%2C+Geoff%3BYellin%2C+Michael+J%3BRosenberg%2C+Steven+A&rft.aulast=Attia&rft.aufirst=Peter&rft.date=2005-09-01&rft.volume=23&rft.issue=25&rft.spage=6043&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-09-01 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Immunol. 1993 Oct 1;151(7):3489-99 [8397258] Immunity. 1994 Aug;1(5):405-13 [7882171] Immunity. 1994 Dec;1(9):793-801 [7534620] Science. 1995 Nov 10;270(5238):985-8 [7481803] Nature. 1987 Jul 16-22;328(6127):267-70 [3496540] Science. 1970 Sep 11;169(3950):1042-9 [4194660] J Immunol. 2004 Aug 15;173(4):2866-76 [15295006] J Immunol. 2003 Dec 1;171(11):5673-7 [14634073] Nat Rev Cancer. 2003 Sep;3(9):666-75 [12951585] J Immunol. 2002 Aug 15;169(4):1852-8 [12165509] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8372-7 [12826605] J Exp Med. 1996 Jun 1;183(6):2533-40 [8676074] J Exp Med. 1996 Jun 1;183(6):2541-50 [8676075] Immunity. 1997 Dec;7(6):885-95 [9430233] Nat Med. 1998 Mar;4(3):321-7 [9500606] Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10067-71 [9707601] Cancer J Sci Am. 1998 Sep-Oct;4(5):316-23 [9815296] Diabetes. 1999 Mar;48(3):652-7 [10078573] J Immunol. 1999 May 15;162(10):5813-20 [10229815] J Exp Med. 1999 Aug 2;190(3):355-66 [10430624] J Immunother. 2004 Nov-Dec;27(6):478-9 [15534492] J Clin Oncol. 2005 Feb 1;23(4):741-50 [15613700] Science. 1996 Mar 22;271(5256):1734-6 [8596936] J Natl Cancer Inst. 2000 Feb 2;92(3):205-16 [10655437] J Exp Med. 2000 Jul 17;192(2):303-10 [10899917] Genes Immun. 2000 Feb;1(3):170-84 [11196709] Annu Rev Immunol. 2001;19:565-94 [11244047] J Exp Med. 2001 Jun 4;193(11):1311-8 [11390438] Eur J Immunol. 2002 Feb;32(2):366-73 [11807776] Nat Rev Immunol. 2001 Dec;1(3):220-8 [11905831] Curr Opin Immunol. 2002 Jun;14(3):391-6 [11973140] Nat Immunol. 2002 Jul;3(7):611-8 [12087419] Nat Rev Immunol. 2002 Jun;2(6):389-400 [12093005] J Immunother. 2003 Jul-Aug;26(4):349-56 [12843797] Nat Immunol. 2003 Apr;4(4):337-42 [12612581] Science. 2003 Feb 14;299(5609):1057-61 [12522256] Science. 1990 Jun 15;248(4961):1349-56 [2113314] J Exp Med. 1991 Mar 1;173(3):759-62 [1847724] J Exp Med. 1991 Mar 1;173(3):721-30 [1847722] J Immunol. 1988 May 15;140(10):3324-30 [2834436] J Exp Med. 1991 Sep 1;174(3):561-9 [1714933] J Immunol. 1992 Jul 15;149(2):380-8 [1320641] Comment In: J Clin Oncol. 2005 Sep 1;23(25):5875-7 [16087939] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Maternal levels of polychlorinated biphenyls in relation to preterm and small-for-gestational-age birth. AN - 68544801; 16135940 AB - In developed countries, polychlorinated biphenyls (PCBs) are ubiquitous contaminants of the environment, including foods. Within the range of the resulting low-level exposure, associations of PCBs with lower birth weight have been observed in several studies. To examine further the association of PCBs with birth outcomes, we measured serum levels in 1034 pregnant women who were enrolled in the U.S. Collaborative Perinatal Project in 1959 to 1965 before PCB manufacturing was banned. The multivariate-adjusted odds ratio for preterm birth among those with PCB levels of >/=4 microg/L of total PCBs, compared with those with <2 microg/L, was 1.1 (95% confidence interval = 0.6-2.2); for the same exposure contrast, the odds ratio for delivering an infant who was small-for-gestational-age at birth was 1.6 (0.7-3.7). Birth weight and length of gestation were essentially unrelated to PCB level. In these data, maternal levels of PCBs during pregnancy were essentially unrelated to preterm birth, birth weight, or length of gestation. An association of PCBs with small-for-gestational-age birth was observed, but the results were inconclusive and occurred in the absence of an overall decrease in birth weight. JF - Epidemiology (Cambridge, Mass.) AU - Longnecker, Matthew P AU - Klebanoff, Mark A AU - Brock, John W AU - Guo, Xuguang AD - Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC, USA. longnecker@niehs.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 641 EP - 647 VL - 16 IS - 5 SN - 1044-3983, 1044-3983 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Prospective Studies KW - Risk Factors KW - Humans KW - Confounding Factors (Epidemiology) KW - Adult KW - Infant, Newborn KW - Infant, Premature KW - United States -- epidemiology KW - Male KW - Female KW - Prenatal Exposure Delayed Effects KW - Pregnancy Outcome KW - Pregnancy KW - Polychlorinated Biphenyls -- blood KW - Environmental Exposure -- adverse effects KW - Infant, Small for Gestational Age KW - Premature Birth UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68544801?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiology+%28Cambridge%2C+Mass.%29&rft.atitle=Maternal+levels+of+polychlorinated+biphenyls+in+relation+to+preterm+and+small-for-gestational-age+birth.&rft.au=Longnecker%2C+Matthew+P%3BKlebanoff%2C+Mark+A%3BBrock%2C+John+W%3BGuo%2C+Xuguang&rft.aulast=Longnecker&rft.aufirst=Matthew&rft.date=2005-09-01&rft.volume=16&rft.issue=5&rft.spage=641&rft.isbn=&rft.btitle=&rft.title=Epidemiology+%28Cambridge%2C+Mass.%29&rft.issn=10443983&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-09-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hair dyes and risk of glioma among Nebraska women. AN - 68535033; 16132796 AB - The etiology of brain cancer is not well understood. We conducted a population-based case-control study among 112 white women in Nebraska who were newly diagnosed with glioma between July 1988 and June 1993, and 215 controls, to identify risk factors for this disease. A 1.7-fold increased risk of glioma was observed for women who ever used hair coloring products (95% confidence interval (CI) = 1.0-2.9, 62 cases), and a 2.4-fold risk for those who used permanent hair coloring products (odds ratio (OR) = 2.4, 95% CI = 1.3-4.5, 39 cases). For women with the most aggressive form of glioma, glioblastoma multiforme, risk increased with duration of exposure to 4.9 (95% CI = 1.6-15.7, 10 cases) after 21 or more years of permanent hair coloring use. Higher risks were observed with earlier age at first use, but we did not see an exposure-response pattern with frequency of use of permanent dyes. No association was observed with use of non-permanent (sometimes called temporary or semi-permanent) hair coloring products. These suggestive findings need confirmation in future studies with larger sample sizes, fewer proxy respondents, and the ability to evaluate the effect of changes in formulations over time. JF - Cancer causes & control : CCC AU - Heineman, Ellen F AU - Ward, Mary H AU - McComb, Rodney D AU - Weisenburger, Dennis D AU - Zahm, Shelia Hoar AD - Division of Cancer Epidemiology and Genetics, NIH, DHHS, National Cancer Institute, Bethesda, MD 20892-7242, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 857 EP - 864 VL - 16 IS - 7 SN - 0957-5243, 0957-5243 KW - Hair Dyes KW - 0 KW - Index Medicus KW - Women's Health KW - Glioblastoma -- etiology KW - Risk Factors KW - Humans KW - Adult KW - Case-Control Studies KW - Middle Aged KW - Female KW - Survival Analysis KW - Risk Assessment KW - Nebraska -- epidemiology KW - Brain Neoplasms -- epidemiology KW - Glioma -- etiology KW - Glioma -- epidemiology KW - Hair Dyes -- adverse effects KW - Brain Neoplasms -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68535033?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+causes+%26+control+%3A+CCC&rft.atitle=Hair+dyes+and+risk+of+glioma+among+Nebraska+women.&rft.au=Heineman%2C+Ellen+F%3BWard%2C+Mary+H%3BMcComb%2C+Rodney+D%3BWeisenburger%2C+Dennis+D%3BZahm%2C+Shelia+Hoar&rft.aulast=Heineman&rft.aufirst=Ellen&rft.date=2005-09-01&rft.volume=16&rft.issue=7&rft.spage=857&rft.isbn=&rft.btitle=&rft.title=Cancer+causes+%26+control+%3A+CCC&rft.issn=09575243&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-07-07 N1 - Date created - 2005-08-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chemotherapy-induced neutropenia and treatment efficacy in advanced non-small-cell lung cancer: a pooled analysis of three randomised trials. AN - 68533293; 16129367 AB - Chemotherapy is the standard treatment for advanced non-small-cell lung cancer, and myelosuppression is a common side-effect. We aimed to assess whether haematological toxic effects could be a biological measure of drug activity and a marker of efficacy. We analysed data for 1265 patients who received chemotherapy (vinorelbine, gemcitabine, gemcitabine and vinorelbine, cisplatin and vinorelbine, or cisplatin and gemcitabine) within three randomised trials. Primary landmark analyses were restricted to 436 patients who received all six planned chemotherapy cycles and who were alive 180 days after randomisation. Neutropenia was categorised on the basis of worst WHO grade during chemotherapy: absent (grade 0), mild (grade 1-2), or severe (grade 3-4). All statistical analyses were stratified by treatment allocation. Analyses were repeated in the out-of-landmark group (829 patients), stratifying by treatment allocation and number of chemotherapy cycles. The primary endpoint was overall survival. In the landmark group, hazard ratios of death were 0.65 (0.46-0.93) for patients with severe neutropenia and 0.74 (0.56-0.98) for those with mild neutropenia. Median survival after the landmark time of 180 days was 31.4 weeks (95% CI 25.7-39.6) for patients without neutropenia compared with 42.0 weeks (32.7-59.7) for patients with severe neutropenia, and with 43.7 weeks (36.6-66.0) for those with mild neutropenia (severe vs mild vs no neutropenia p=0.0118). Findings were much the same for the out-of-landmark group. Neutropenia during chemotherapy is associated with increased survival of patients with advanced non-small-cell lung cancer, and its absence might be a result of underdosing. Prospective trials are needed to assess whether drug dosing guided by the occurrence of toxic effects could improve efficacy of standard regimens. JF - The Lancet. Oncology AU - Di Maio, Massimo AU - Gridelli, Cesare AU - Gallo, Ciro AU - Shepherd, Frances AU - Piantedosi, Franco Vito AU - Cigolari, Silvio AU - Manzione, Luigi AU - Illiano, Alfonso AU - Barbera, Santi AU - Robbiati, Sergio Federico AU - Frontini, Luciano AU - Piazza, Elena AU - Ianniello, Giovanni Pietro AU - Veltri, Enzo AU - Castiglione, Federico AU - Rosetti, Francesco AU - Gebbia, Vittorio AU - Seymour, Lesley AU - Chiodini, Paolo AU - Perrone, Francesco AD - National Cancer Institute, Naples, Italy. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 669 EP - 677 VL - 6 IS - 9 SN - 1470-2045, 1470-2045 KW - Antineoplastic Agents KW - 0 KW - Biomarkers KW - Index Medicus KW - Severity of Illness Index KW - Randomized Controlled Trials as Topic KW - Survival Rate KW - Dose-Response Relationship, Drug KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Italy -- epidemiology KW - Male KW - Female KW - Proportional Hazards Models KW - Neutropenia -- blood KW - Carcinoma, Non-Small-Cell Lung -- mortality KW - Antineoplastic Agents -- administration & dosage KW - Neutropenia -- epidemiology KW - Lung Neoplasms -- drug therapy KW - Drug Monitoring KW - Neutropenia -- chemically induced KW - Lung Neoplasms -- mortality KW - Neutropenia -- diagnosis KW - Carcinoma, Non-Small-Cell Lung -- drug therapy KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68533293?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Lancet.+Oncology&rft.atitle=Chemotherapy-induced+neutropenia+and+treatment+efficacy+in+advanced+non-small-cell+lung+cancer%3A+a+pooled+analysis+of+three+randomised+trials.&rft.au=Di+Maio%2C+Massimo%3BGridelli%2C+Cesare%3BGallo%2C+Ciro%3BShepherd%2C+Frances%3BPiantedosi%2C+Franco+Vito%3BCigolari%2C+Silvio%3BManzione%2C+Luigi%3BIlliano%2C+Alfonso%3BBarbera%2C+Santi%3BRobbiati%2C+Sergio+Federico%3BFrontini%2C+Luciano%3BPiazza%2C+Elena%3BIanniello%2C+Giovanni+Pietro%3BVeltri%2C+Enzo%3BCastiglione%2C+Federico%3BRosetti%2C+Francesco%3BGebbia%2C+Vittorio%3BSeymour%2C+Lesley%3BChiodini%2C+Paolo%3BPerrone%2C+Francesco&rft.aulast=Di+Maio&rft.aufirst=Massimo&rft.date=2005-09-01&rft.volume=6&rft.issue=9&rft.spage=669&rft.isbn=&rft.btitle=&rft.title=The+Lancet.+Oncology&rft.issn=14702045&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-08-30 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Lancet Oncol. 2005 Sep;6(9):637-8 [16129364] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Caveolin-1 is expressed on multipotent cells of hair follicles and might be involved in their resistance to chemotherapy. AN - 68516613; 16120134 AB - Caveolin-1 is the principal protein that composes caveolae, which are vesicular invaginations present on the plasma membrane of different cell types. Caveolae are involved in a variety of cellular functions including regulation of proliferation rate and resistance to chemotherapeutic drugs. Chemotherapy frequently induces alopecia which is reversible most probably due to the low proliferative rate of hair follicle stem cells and due to the expression of proteins which confer resistance. Using a specific animal model and immunohistochemistry, we analysed the expression of both caveolin-1 and the cell proliferation marker beta-catenin, at different stages of the hair follicle cycle, both before and after doxorubicin (DXR) -induced alopecia. Seven-week-old C57BL/6 mice were depilated in order to synchronize hair follicle cycle in the anagen phase. Chemotherapy with DXR 15 mg kg(-1) was used to induce alopecia. Control and treated mice were then sacrificed at precise time points and caveolin-1 expression in hairs at different stages of the cycle were analysed by immunohistochemistry. By double immunofluorescence, colocalization of caveolin-1 and cytokeratin-15 was confirmed in the bulge region. The state of proliferation of cells composing hair follicle was assessed by beta-catenin immunohistochemistry. Caveolin-1 was expressed by the cells of the bulge area, the multipotent compartment of the hair follicle, during all phases of growth (anagen), regression (catagen) and resting (telogen). During the anagen phases, nuclear beta-catenin labelling was not observed in bulge cells, but rather in the deeper portion of the follicle. Damaged hair follicles from DXR-treated mice presented bulge cells which still expressed caveolin-1, suggesting that this protein might play a role in their drug resistance. As expected, no beta-catenin nuclear staining was detectable in DXR-treated hair follicles, indicating the complete lack of proliferative processes. The differential localization of caveolin-1 and beta-catenin suggests that the mutually exclusive expression of these proteins is useful for correct hair regrowth, whether during the physiological cycle or after chemotherapy-induced alopecia. Expression of caveolin-1 within the multipotent cell compartment of the hair follicle can explain the resistance of bulge cells to many chemotherapeutics, suggested by the reversibility of chemotherapy-induced alopecia. JF - The British journal of dermatology AU - Selleri, S AU - Arnaboldi, F AU - Palazzo, M AU - Hussein, U AU - Balsari, A AU - Rumio, C AD - Department of Human Morphology, National Cancer Institute, Università degli Studi di Milano, Via Mangiagalli, 31, 20133 Milan, Italy. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 506 EP - 513 VL - 153 IS - 3 SN - 0007-0963, 0007-0963 KW - Antineoplastic Agents KW - 0 KW - Biomarkers KW - CTNNB1 protein, mouse KW - Cav1 protein, mouse KW - Caveolin 1 KW - Caveolins KW - Cytoskeletal Proteins KW - Trans-Activators KW - beta Catenin KW - Doxorubicin KW - 80168379AG KW - Index Medicus KW - Models, Animal KW - Animals KW - Doxorubicin -- adverse effects KW - Trans-Activators -- analysis KW - Drug Resistance KW - Immunohistochemistry -- methods KW - Mice KW - Cytoskeletal Proteins -- analysis KW - Cell Proliferation KW - Antineoplastic Agents -- adverse effects KW - Biomarkers -- analysis KW - Mice, Inbred C57BL KW - Hair Removal KW - Alopecia -- metabolism KW - Hair Follicle -- pathology KW - Caveolins -- metabolism KW - Multipotent Stem Cells -- metabolism KW - Multipotent Stem Cells -- drug effects KW - Caveolins -- analysis KW - Alopecia -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68516613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+British+journal+of+dermatology&rft.atitle=Caveolin-1+is+expressed+on+multipotent+cells+of+hair+follicles+and+might+be+involved+in+their+resistance+to+chemotherapy.&rft.au=Selleri%2C+S%3BArnaboldi%2C+F%3BPalazzo%2C+M%3BHussein%2C+U%3BBalsari%2C+A%3BRumio%2C+C&rft.aulast=Selleri&rft.aufirst=S&rft.date=2005-09-01&rft.volume=153&rft.issue=3&rft.spage=506&rft.isbn=&rft.btitle=&rft.title=The+British+journal+of+dermatology&rft.issn=00070963&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-14 N1 - Date created - 2005-08-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Exposure to chronic high glucose induces beta-cell apoptosis through decreased interaction of glucokinase with mitochondria: downregulation of glucokinase in pancreatic beta-cells. AN - 68513668; 16123348 AB - Chronic hyperglycemia is toxic to pancreatic beta-cells, impairing cellular functioning as observed in type 2 diabetes; however, the mechanism underlying beta-cell dysfunction and the resulting apoptosis via glucose toxicity are not fully characterized. Here, using MIN6N8 cells, a mouse pancreatic beta-cell line, we show that chronic exposure to high glucose increases cell death mediated by Bax oligomerization, cytochrome C release, and caspase-3 activation. During apoptosis, glucokinase (GCK) expression decreases in high-glucose-treated cells, concomitant with a decrease in cellular ATP production and insulin secretion. Moreover, exposure to a chronically high dose of glucose decreases interactions between GCK and mitochondria with an increase in Bax binding to mitochondria and cytochrome C release. These events are prevented by GCK overexpression, and phosphorylation of proapoptotic Bad proteins in GCK-overexpressing cells is prolonged compared with Neo-transfected cells. Similar results are obtained using primary islet cells. Collectively, these data demonstrate that beta-cell apoptosis from exposure to chronic high glucose occurs in relation to lowered GCK expression and reduced association with mitochondria. Our results show that this may be one mechanism by which glucose is toxic to beta-cells and suggests a novel approach to prevent and treat diabetes by manipulating Bax- and GCK-controlled signaling to promote apoptosis or proliferation. JF - Diabetes AU - Kim, Won-Ho AU - Lee, June Woo AU - Suh, Young Ho AU - Hong, Shin Hee AU - Choi, Joo Sun AU - Lim, Joo Hyun AU - Song, Ji Hyun AU - Gao, Bin AU - Jung, Myeong Ho AD - Division of Metabolic Disease, Department of Biomedical Science, National Institutes of Health, #5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 2602 EP - 2611 VL - 54 IS - 9 SN - 0012-1797, 0012-1797 KW - Cytochromes c KW - 9007-43-6 KW - Glucokinase KW - EC 2.7.1.2 KW - Glucose KW - IY9XDZ35W2 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Down-Regulation KW - Cytochromes c -- physiology KW - Cell Line, Tumor KW - Time Factors KW - Mitochondria -- physiology KW - Glucose -- physiology KW - Apoptosis -- drug effects KW - Glucokinase -- metabolism KW - Islets of Langerhans -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68513668?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diabetes&rft.atitle=Exposure+to+chronic+high+glucose+induces+beta-cell+apoptosis+through+decreased+interaction+of+glucokinase+with+mitochondria%3A+downregulation+of+glucokinase+in+pancreatic+beta-cells.&rft.au=Kim%2C+Won-Ho%3BLee%2C+June+Woo%3BSuh%2C+Young+Ho%3BHong%2C+Shin+Hee%3BChoi%2C+Joo+Sun%3BLim%2C+Joo+Hyun%3BSong%2C+Ji+Hyun%3BGao%2C+Bin%3BJung%2C+Myeong+Ho&rft.aulast=Kim&rft.aufirst=Won-Ho&rft.date=2005-09-01&rft.volume=54&rft.issue=9&rft.spage=2602&rft.isbn=&rft.btitle=&rft.title=Diabetes&rft.issn=00121797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-21 N1 - Date created - 2005-08-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lineage-specific engraftment and outcomes after T-cell-depleted peripheral blood stem cell transplant with Flu/Cy/TBI conditioning. AN - 68510274; 16115130 AB - Sixty patients with haematological malignancies received a myeloablative regimen of total body irradiation, cyclophosphamide and fludarabine followed by a T-cell-depleted peripheral blood stem cell transplant from a human leucocyte antigen identical sibling. To improve donor immune function, 1 x 10(7) CD3+ cells/kg were added-back between d 45 and 100. T-cell and myeloid chimaerism were monitored regularly to evaluate the effect of T-cell chimaerism on outcome. The major factor affecting outcome was disease risk, with significantly lower relapse and higher survival in 29 standard risk (SR) patients compared with 31 patients at high risk (HR) for treatment failure (relapse 4.8 +/- 5% vs. 59 +/- 11%, P or = 95% within 14 d of transplant, but in the first several months, donor T-cell chimaerism was frequently mixed. Full T-cell chimaerism was significantly more frequent in HR vs. SR patients. Landmark analysis at days 30 and 90 in HR patients with mixed versus full T-cell chimaerism, showed relapse probabilities of 50.5 +/- 14% vs. 70 +/- 16% (P = 0.62) and 34.4 +/- 20% vs. 58.8 +/- 15% (P = 0.32) respectively. Early full T-cell engraftment correlated with development of severe acute graft-versus-host disease (GVHD). However, mixed T-cell chimaerism was favourable for reducing GVHD, and did not affect relapse in this small series. JF - British journal of haematology AU - Montero, Aldemar AU - Savani, Bipin N AU - Kurlander, Roger AU - Read, Elizabeth J AU - Leitman, Susan F AU - Childs, Richard AU - Solomon, Scott R AU - Barrett, A John AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1202, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 733 EP - 739 VL - 130 IS - 5 SN - 0007-1048, 0007-1048 KW - Immunosuppressive Agents KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Vidarabine KW - FA2DM6879K KW - fludarabine KW - P2K93U8740 KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Vidarabine -- analogs & derivatives KW - Transplantation Conditioning KW - Humans KW - Transplantation Chimera KW - Child KW - Vidarabine -- administration & dosage KW - Survival Rate KW - Lymphocyte Transfusion KW - Adult KW - Graft vs Host Disease KW - Follow-Up Studies KW - Adolescent KW - Female KW - Male KW - Proportional Hazards Models KW - Hematologic Neoplasms -- therapy KW - Whole-Body Irradiation KW - Peripheral Blood Stem Cell Transplantation KW - Lymphocyte Depletion KW - Hematologic Neoplasms -- immunology KW - Immunosuppressive Agents -- therapeutic use KW - T-Lymphocytes KW - Hematologic Neoplasms -- mortality UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68510274?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+journal+of+haematology&rft.atitle=Lineage-specific+engraftment+and+outcomes+after+T-cell-depleted+peripheral+blood+stem+cell+transplant+with+Flu%2FCy%2FTBI+conditioning.&rft.au=Montero%2C+Aldemar%3BSavani%2C+Bipin+N%3BKurlander%2C+Roger%3BRead%2C+Elizabeth+J%3BLeitman%2C+Susan+F%3BChilds%2C+Richard%3BSolomon%2C+Scott+R%3BBarrett%2C+A+John&rft.aulast=Montero&rft.aufirst=Aldemar&rft.date=2005-09-01&rft.volume=130&rft.issue=5&rft.spage=733&rft.isbn=&rft.btitle=&rft.title=British+journal+of+haematology&rft.issn=00071048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-08-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - (+/-)-3,4-Methylenedioxymethamphetamine administration to rats does not decrease levels of the serotonin transporter protein or alter its distribution between endosomes and the plasma membrane. AN - 68503741; 15937150 AB - We showed that the serotonin (5-HT) neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) reduces brain tissue 5-HT, decreases expression of 5-HT transporter (SERT) protein, and increases expression of glial fibrillary acidic protein (GFAP). In contrast, doses of (+/-)-3,4-methylenedioxymethamphetamine (MDMA) that decrease brain tissue 5-HT fail to alter expression of SERT or GFAP. Using a new and highly sensitive anti-SERT antibody, we determined whether MDMA alters the subcellular distribution of SERT protein by measuring SERT expression in endosomes and plasma membranes 2 weeks after MDMA administration. Rat brain tissues (caudate, cortex, and hippocampus) were collected 3 days and 2 weeks after MDMA (7.5 mg/kg i.p., every 2 h x 3 doses) or 5,7-DHT (150 microg/rat i.c.v.) administration. Representative results from cortex are as follows. At both 3 days and 2 weeks postinjection, MDMA decreased tissue 5-HT (65%) and had no effect on GFAP expression. MDMA increased heat shock protein 32 (HSP32; a marker for microglial activation) expression (30%) at 3 days, but not 2 weeks. MDMA did not alter SERT expression at either time point and did not alter SERT levels in either endosomes or plasma membranes (2 weeks). 5,7-DHT decreased tissue 5-HT (80%), increased HSP32 expression at both time points (about 50%), and increased GFAP expression at 2 weeks (40%). 5,7-DHT decreased SERT expression (33%) at 2 weeks, but not at 3 days. These findings indicate that a dosing regimen of MDMA that depletes brain 5-HT does not alter SERT protein expression or the distribution of SERT between endosomes and the plasma membrane and does not produce detectable evidence for neurotoxicity. JF - The Journal of pharmacology and experimental therapeutics AU - Wang, Xiaoying AU - Baumann, Michael H AU - Xu, Heng AU - Morales, Marisela AU - Rothman, Richard B AD - Clinical Psychopharmacology Section, Intramural Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1002 EP - 1012 VL - 314 IS - 3 SN - 0022-3565, 0022-3565 KW - Glial Fibrillary Acidic Protein KW - 0 KW - Membrane Glycoproteins KW - Membrane Transport Proteins KW - Nerve Tissue Proteins KW - Serotonin Plasma Membrane Transport Proteins KW - Slc6a4 protein, rat KW - 5,7-Dihydroxytryptamine KW - 31363-74-3 KW - Serotonin KW - 333DO1RDJY KW - Heme Oxygenase (Decyclizing) KW - EC 1.14.14.18 KW - Hmox1 protein, rat KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Index Medicus KW - Rats KW - Heme Oxygenase (Decyclizing) -- analysis KW - Animals KW - Rats, Sprague-Dawley KW - Serotonin -- analysis KW - Neurons -- chemistry KW - Neuroglia -- chemistry KW - Glial Fibrillary Acidic Protein -- analysis KW - 5,7-Dihydroxytryptamine -- pharmacology KW - Male KW - Nerve Tissue Proteins -- analysis KW - Nerve Tissue Proteins -- drug effects KW - Membrane Glycoproteins -- drug effects KW - Nerve Tissue Proteins -- metabolism KW - Membrane Transport Proteins -- drug effects KW - Membrane Glycoproteins -- analysis KW - Endosomes -- chemistry KW - Cell Membrane -- chemistry KW - N-Methyl-3,4-methylenedioxyamphetamine -- pharmacology KW - Membrane Transport Proteins -- metabolism KW - Membrane Transport Proteins -- analysis KW - Membrane Glycoproteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68503741?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Analyses+of+Recombinant+Vaccinia+and+Fowlpox+Vaccine+Vectors+Expressing+Transgenes+for+Two+Human+Tumor+Antigens+and+Three+Human+Costimulatory+Molecules&rft.au=Tsang%2C+Kwong+Y%3BPalena%2C+Claudia%3BYokokawa%2C+Junko%3BArlen%2C+Philip+M%3BGulley%2C+James+L%3BMazzara%2C+Gail+P%3BGritz%2C+Linda%3BGomez+Yafal%2C+Alicia%3BOgueta%2C+Sandra%3BGreenhalgh%2C+Patricia%3BManson%2C+Kelledy%3BPanicali%2C+Dennis%3BSchlom%2C+Jeffrey&rft.aulast=Tsang&rft.aufirst=Kwong&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1597&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-21 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The dopamine D3 receptor and drug dependence: effects on reward or beyond? AN - 68501929; 15963538 AB - Abused drugs (alcohol, heroin, cocaine, tetrahydrocannabinol and nicotine) elicit a variety of chronically relapsing disorders by interacting with brain reward systems. All of these drugs increase dopamine levels in the shell of nucleus accumbens, a structure that has been involved in their hedonic and reinforcing properties. Dopamine D(3) receptors (DRD3) are predominantly expressed in the nucleus accumbens, but also in the ventral tegmental area and in the amygdala, brain structures implicated in drug dependence. Moreover, converging pharmacological, human post-mortem and genetic studies have suggested the involvement of the DRD3 in drug dependence. Based on early studies using non-selective DRD3 ligands, the DRD3 was proposed as having a direct role in the rewarding effects of psychostimulants. However, recent studies using highly selective DRD3 ligands and the DRD3-deficient mice have revealed that the DRD3 is not implicated in the direct reinforcing effects of drugs of abuse. In contrast, the DRD3 appears to be implicated in the motivation to self-administer drugs under schedules where the response requirements are high. This is consistent with a behavioral economic analysis, with the effects of DRD3 ligands revealed only in situations with high prices for drug. Drug-self administration and relapse are strongly controlled by environmental stimuli. The DRD3 strongly modulates the influence of these environmental stimuli on drug-seeking behavior. DRD3 blockade disrupts the reactivity to drug-associated stimuli in various paradigms, such as second-order schedules of drug-self administration, conditioned place preference and Pavlovian conditioning procedures. In several paradigms, the involvement of the DRD3 has been confirmed by using DRD3-deficient mice. On the contrary, reactivity to stimuli associated with natural reinforcers, such as food, appears unaffected by modulation of the DRD3. All these findings suggest that DRD3 ligands may represent a useful strategy for decreasing relapse in abstinent drug-abusers. JF - Neuropharmacology AU - Le Foll, Bernard AU - Goldberg, Steven R AU - Sokoloff, Pierre AD - Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, National Institutes of Health/DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. blefoll@intra.nida.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 525 EP - 541 VL - 49 IS - 4 SN - 0028-3908, 0028-3908 KW - Dopamine Agents KW - 0 KW - Receptors, Dopamine D3 KW - Index Medicus KW - Dopamine Agents -- therapeutic use KW - Animals KW - Humans KW - Dopamine Agents -- pharmacology KW - Brain -- anatomy & histology KW - Gene Expression Regulation -- drug effects KW - Brain -- metabolism KW - Substance-Related Disorders -- physiopathology KW - Receptors, Dopamine D3 -- physiology KW - Reward KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68501929?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropharmacology&rft.atitle=The+dopamine+D3+receptor+and+drug+dependence%3A+effects+on+reward+or+beyond%3F&rft.au=Le+Foll%2C+Bernard%3BGoldberg%2C+Steven+R%3BSokoloff%2C+Pierre&rft.aulast=Le+Foll&rft.aufirst=Bernard&rft.date=2005-09-01&rft.volume=49&rft.issue=4&rft.spage=525&rft.isbn=&rft.btitle=&rft.title=Neuropharmacology&rft.issn=00283908&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hormonal correlates of clozapine-induced weight gain in psychotic children: an exploratory study. AN - 68496232; 16113621 AB - Weight gain is a serious side effect of atypical antipsychotics, especially in childhood. In this study, the authors examined six weight gain-related hormones in patients with childhood-onset schizophrenia (COS) after 6 weeks of clozapine treatment. Fasting serum samples for 24 patients with COS and 21 matched healthy controls (HC) were obtained. Levels of leptin, insulin, adiponectin, amylin, ghrelin, and tumor necrosis factor alpha were measured and compared between the groups. For 23 patients with COS, hormonal levels were measured at background and week 6 of clozapine treatment. Change in body mass index was correlated with levels of clozapine and changes in hormonal levels and clinical ratings. At baseline, COS did not differ significantly from HC on any hormonal measure. Clozapine treatment was associated with significant (7.9% +/- 8.5%) increase in mean body mass index. Only leptin levels increased significantly from baseline to week 6 on clozapine (p = .003). Body mass index increase was significantly correlated with decrease in ghrelin and adiponectin and was positively correlated with clinical improvement. This is the first study of weight gain-related hormones in children on clozapine. Hormonal changes are correlated with weight gain. How effectiveness of clozapine is linked to weight gain remains uncertain. JF - Journal of the American Academy of Child and Adolescent Psychiatry AU - Sporn, Alexandra L AU - Bobb, Aaron J AU - Gogtay, Nitin AU - Stevens, Hanna AU - Greenstein, Deanna K AU - Clasen, Liv S AU - Tossell, Julia W AU - Nugent, Thomas AU - Gochman, Peter A AU - Sharp, Wendy S AU - Mattai, Anand AU - Lenane, Marge C AU - Yanovski, Jack A AU - Rapoport, Judith L AD - Child Psychiatry Branch, NIMH, NIH, Bethesda, MD, USA. sporna@intra.nimh.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 925 EP - 933 VL - 44 IS - 9 SN - 0890-8567, 0890-8567 KW - Antipsychotic Agents KW - 0 KW - Hormones KW - Clozapine KW - J60AR2IKIC KW - Index Medicus KW - Humans KW - Hormones -- blood KW - Schizophrenic Psychology KW - Child KW - Body Mass Index KW - Adolescent KW - Male KW - Female KW - Weight Gain -- drug effects KW - Schizophrenia -- blood KW - Schizophrenia -- drug therapy KW - Clozapine -- adverse effects KW - Antipsychotic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68496232?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=HA22+%28R490A%29+Is+a+Recombinant+Immunotoxin+with+Increased+Antitumor+Activity+without+an+Increase+in+Animal+Toxicity&rft.au=Bang%2C+Sookhee%3BNagata%2C+Satoshi%3BOnda%2C+Masanori%3BKreitman%2C+Robert+J%3BPastan%2C+Ira&rft.aulast=Bang&rft.aufirst=Sookhee&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1545&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-09 N1 - Date created - 2005-08-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Smoky coal exposure, NBS1 polymorphisms, p53 protein accumulation, and lung cancer risk in Xuan Wei, China. AN - 68484145; 15921821 AB - Lung cancer rates in Xuan Wei County are among the highest in China and have been associated with exposure to indoor smoky coal emissions that contain high levels of polycyclic aromatic hydrocarbons (PAHs). The NBS1 gene product participates in DNA double-strand break repair and DNA damage-induced checkpoint activation, which are critical for maintaining genomic integrity. The p53 tumor suppressor gene is known to play key roles both in the maintenance of genomic stability in mammalian cells and in DNA damage surveillance. We examined the association between two common NBS1 polymorphisms (Leu34Leu, Gln185Glu) and lung cancer risk in a population-based case-control study in Xuan Wei, China. Individuals homozygous for the NBS1 34Leu or NBS1 185Glu variants were found to have an increased risk of lung cancer (odds ratio [OR] 2.15, 95% confidence interval [CI]: 0.91-5.10 and OR 2.53, 95% CI: 1.05-6.08, respectively). A haplotype containing the variant alleles from both NBS1 SNPs was associated with increased risk of lung cancer compared with the most common haplotype. Further, the associations were particularly pronounced among cases with over expression of p53 protein. These results suggest that NBS1 could be important in the pathogenesis of lung cancer in this population. However, additional studies in other populations with substantial environmental exposures to PAHs are needed to confirm our findings. JF - Lung cancer (Amsterdam, Netherlands) AU - Lan, Qing AU - Shen, Min AU - Berndt, Sonja I AU - Bonner, Matthew R AU - He, Xingzhou AU - Yeager, Meredith AU - Welch, Robert AU - Keohavong, Phouthone AU - Donahue, Mark AU - Hainaut, Pierre AU - Chanock, Stephen AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, MSC 7240, 6120 Executive Blvd., EPS 8109, Bethesda, MD 20892-7240, USA. qingl@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 317 EP - 323 VL - 49 IS - 3 SN - 0169-5002, 0169-5002 KW - Cell Cycle Proteins KW - 0 KW - Coal KW - NBN protein, human KW - Nuclear Proteins KW - Polycyclic Aromatic Hydrocarbons KW - Tumor Suppressor Protein p53 KW - Index Medicus KW - Genetic Variation KW - Odds Ratio KW - Homozygote KW - DNA Repair KW - Genome, Human KW - DNA Damage KW - Humans KW - Aged KW - Polycyclic Aromatic Hydrocarbons -- adverse effects KW - Genotype KW - Risk KW - Alleles KW - Haplotypes KW - Adult KW - Case-Control Studies KW - Environmental Exposure KW - Middle Aged KW - Male KW - Female KW - Tumor Suppressor Protein p53 -- biosynthesis KW - Lung Neoplasms -- diagnosis KW - Nuclear Proteins -- genetics KW - Coal -- adverse effects KW - Cell Cycle Proteins -- genetics KW - Polymorphism, Genetic KW - Lung Neoplasms -- genetics KW - Tumor Suppressor Protein p53 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68484145?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.atitle=Smoky+coal+exposure%2C+NBS1+polymorphisms%2C+p53+protein+accumulation%2C+and+lung+cancer+risk+in+Xuan+Wei%2C+China.&rft.au=Lan%2C+Qing%3BShen%2C+Min%3BBerndt%2C+Sonja+I%3BBonner%2C+Matthew+R%3BHe%2C+Xingzhou%3BYeager%2C+Meredith%3BWelch%2C+Robert%3BKeohavong%2C+Phouthone%3BDonahue%2C+Mark%3BHainaut%2C+Pierre%3BChanock%2C+Stephen&rft.aulast=Lan&rft.aufirst=Qing&rft.date=2005-09-01&rft.volume=49&rft.issue=3&rft.spage=317&rft.isbn=&rft.btitle=&rft.title=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.issn=01695002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-23 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inactivation of FGF8 in early mesoderm reveals an essential role in kidney development. AN - 68479696; 16049111 AB - To bypass the essential gastrulation function of Fgf8 and study its role in lineages of the primitive streak, we have used a new mouse line, T-Cre, to generate mouse embryos with pan-mesodermal loss of Fgf8 expression. Surprisingly, despite previous models in which Fgf8 has been assigned a pivotal role in segmentation/somite differentiation, Fgf8 is not required for these processes. However, mutant neonates display severe renal hypoplasia with deficient nephron formation. In mutant kidneys, aberrant cell death occurs within the metanephric mesenchyme (MM), particularly in the cortical nephrogenic zone, which provides the progenitors for recurring rounds of nephron formation. Prior to mutant morphological changes, Wnt4 and Lim1 expression, which is essential for nephrogenesis, is absent in MM. Furthermore, comparative analysis of Wnt4-null homozygotes reveals concomitant downregulation of Lim1 and diminished tubule formation. Our data support a model whereby FGF8 and WNT4 function in concert to induce the expression of Lim1 for MM survival and tubulogenesis. JF - Development (Cambridge, England) AU - Perantoni, Alan O AU - Timofeeva, Olga AU - Naillat, Florence AU - Richman, Charmaine AU - Pajni-Underwood, Sangeeta AU - Wilson, Catherine AU - Vainio, Seppo AU - Dove, Lee F AU - Lewandoski, Mark AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute, NCI-Frederick, Frederick, MD 21702, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 3859 EP - 3871 VL - 132 IS - 17 SN - 0950-1991, 0950-1991 KW - Fgf8 protein, mouse KW - 0 KW - Homeodomain Proteins KW - LIM-Homeodomain Proteins KW - Lhx1 protein, mouse KW - Proto-Oncogene Proteins KW - Transcription Factors KW - Wnt Proteins KW - Wnt4 Protein KW - Wnt4 protein, mouse KW - Fibroblast Growth Factor 8 KW - 148997-75-5 KW - Fibroblast Growth Factors KW - 62031-54-3 KW - Index Medicus KW - Animals KW - Limb Buds -- cytology KW - Limb Buds -- metabolism KW - Cell Lineage KW - Proto-Oncogene Proteins -- metabolism KW - Cell Differentiation KW - Mice KW - Limb Buds -- embryology KW - Mice, Transgenic KW - Gene Deletion KW - Alleles KW - Apoptosis -- genetics KW - Proto-Oncogene Proteins -- deficiency KW - Homeodomain Proteins -- metabolism KW - Mutation -- genetics KW - Proto-Oncogene Proteins -- genetics KW - Time Factors KW - Signal Transduction KW - Gene Expression Regulation, Developmental KW - Kidney -- metabolism KW - Kidney -- embryology KW - Kidney -- cytology KW - Mesoderm -- cytology KW - Mesoderm -- metabolism KW - Fibroblast Growth Factors -- metabolism KW - Fibroblast Growth Factors -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479696?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Development+%28Cambridge%2C+England%29&rft.atitle=Inactivation+of+FGF8+in+early+mesoderm+reveals+an+essential+role+in+kidney+development.&rft.au=Perantoni%2C+Alan+O%3BTimofeeva%2C+Olga%3BNaillat%2C+Florence%3BRichman%2C+Charmaine%3BPajni-Underwood%2C+Sangeeta%3BWilson%2C+Catherine%3BVainio%2C+Seppo%3BDove%2C+Lee+F%3BLewandoski%2C+Mark&rft.aulast=Perantoni&rft.aufirst=Alan&rft.date=2005-09-01&rft.volume=132&rft.issue=17&rft.spage=3859&rft.isbn=&rft.btitle=&rft.title=Development+%28Cambridge%2C+England%29&rft.issn=09501991&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Differential splicing of E6 within human papillomavirus type 18 variants and functional consequences. AN - 68479210; 16099904 AB - Persistent infections of the uterine cervix with 'high-risk' human papillomavirus (HPV) are now recognized as necessary for the development of cervical cancer. Among them, HPV types 16 and 18 exhibit numerous variants associated with different risks for cervical cancer development. In this study, the questions of whether different HPV type 18 variants exhibit changes in early gene transcription and the molecular mechanisms underlying these differences were investigated. It was shown that, indeed, type 18 variants exhibited singular differences in E6 transcripts in vivo. Higher levels of the E6*I transcript were detected regularly in clones harbouring the African variant, as opposed to low levels of this transcript detected in clones containing the reference clone (Asian-Amerindian), where significantly higher levels of full-length E6 transcript were usually observed. As a direct consequence, higher levels of p53 protein were found in the presence of African E6, as opposed to the low levels of p53 observed with the Asian-Amerindian E6. These variations in consequence affected the levels of cellular proteins regulated by p53, such as Bax. Similar changes in the relative levels of E6 transcripts were observed when tumours containing type 18 E6 variants were analysed. The different ability of cells containing variant E6 genes to form tumours in nude mice was suggested by the fact that tumour volumes were considerably higher when cells expressed the Asian-Amerindian E6. Mutagenesis analysis of the reference clone showed that a C491A change reverts the phenotype. These results suggest that different splicing patterns of E6 within HPV type 18 variants may possibly have biological implications in viral tumorigenesis. JF - The Journal of general virology AU - De la Cruz-Hernández, Erick AU - García-Carrancá, Alejandro AU - Mohar-Betancourt, Alejandro AU - Dueñas-González, Alfonso AU - Contreras-Paredes, Adriana AU - Pérez-Cardenas, Enrique AU - Herrera-Goepfert, Roberto AU - Lizano-Soberón, Marcela AD - Unit of Biomedical Research in Cancer, National Cancer Institute/Biomedical Research Institute, National Autonomous University of Mexico, Av. San Fernando No. 22, Col. Sección 16, Tlalpan, 14080 Mexico City, Mexico. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 2459 EP - 2468 VL - 86 SN - 0022-1317, 0022-1317 KW - DNA-Binding Proteins KW - 0 KW - E6 protein, Human papillomavirus type 18 KW - Oncogene Proteins, Viral KW - Index Medicus KW - Genetic Variation KW - Animals KW - HeLa Cells KW - Humans KW - Mice, Nude KW - Mice KW - Mice, Inbred BALB C KW - NIH 3T3 Cells KW - Female KW - Cell Line KW - Papillomavirus Infections -- physiopathology KW - Papillomaviridae -- pathogenicity KW - Papillomaviridae -- classification KW - RNA Splicing KW - Papillomavirus Infections -- virology KW - DNA-Binding Proteins -- genetics KW - Uterine Cervical Neoplasms -- physiopathology KW - Oncogene Proteins, Viral -- genetics KW - Papillomaviridae -- genetics KW - Oncogene Proteins, Viral -- metabolism KW - Uterine Cervical Neoplasms -- virology KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479210?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+general+virology&rft.atitle=Differential+splicing+of+E6+within+human+papillomavirus+type+18+variants+and+functional+consequences.&rft.au=De+la+Cruz-Hern%C3%A1ndez%2C+Erick%3BGarc%C3%ADa-Carranc%C3%A1%2C+Alejandro%3BMohar-Betancourt%2C+Alejandro%3BDue%C3%B1as-Gonz%C3%A1lez%2C+Alfonso%3BContreras-Paredes%2C+Adriana%3BP%C3%A9rez-Cardenas%2C+Enrique%3BHerrera-Goepfert%2C+Roberto%3BLizano-Sober%C3%B3n%2C+Marcela&rft.aulast=De+la+Cruz-Hern%C3%A1ndez&rft.aufirst=Erick&rft.date=2005-09-01&rft.volume=86&rft.issue=&rft.spage=2459&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+general+virology&rft.issn=00221317&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chromosomal aberrations in cell lines derived from thyroid tumors spontaneously developed in TRbetaPV/PV mice. AN - 68478155; 16102579 AB - The etiology and genetic alterations of follicular thyroid carcinoma are not well understood. By targeting a mutation (PV) into the thyroid hormone receptor beta gene (TRbetaPV mouse), we created a knock-in mutant TRbeta(PV/PV) mouse that spontaneously develop follicular thyroid carcinoma with progression to metastasis similar to human follicular thyroid carcinoma. This mouse model provides a valuable tool to ascertain the nature and the extent of genomic rearrangements that occur during carcinogenesis of the thyroid. Spectral karyotyping analysis (SKY) of seven cell lines derived from thyroid tumors developed in TRbeta(PV/PV) mice showed that all of them had abnormal karyotypes, with chromosome number ranging from near-diploid (39-42 chromosomes) to hypotetraploid (63-79 chromosomes). These seven cell lines also exhibited a variety of structural chromosomal aberrations, including common recurrent translocations and deletions. This SKY analysis shows that the development and progression of follicular thyroid carcinoma in knock-in TRbeta(PV/PV) mutant mice comprise recurrent structural and numerical genomic changes, some of which mimic those described in human thyroid cancer. JF - Cancer genetics and cytogenetics AU - Zimonjic, Drazen B AU - Kato, Yasuhito AU - Ying, Hao AU - Popescu, Nicholas C AU - Cheng, Sheue-Yann AD - Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, 37 Convent Drive MSC 4262, Building 37/Room 4128C, Bethesda, MD 20892-4262, USA. drazen_zimonjic@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 104 EP - 109 VL - 161 IS - 2 SN - 0165-4608, 0165-4608 KW - Thyroid Hormone Receptors beta KW - 0 KW - Thyroglobulin KW - 9010-34-8 KW - Index Medicus KW - Animals KW - Mice, Mutant Strains KW - Thyroglobulin -- analysis KW - Mice KW - Cell Line, Tumor KW - Mutation KW - Spectral Karyotyping KW - Thyroid Neoplasms -- genetics KW - Adenocarcinoma, Follicular -- metabolism KW - Thyroid Neoplasms -- metabolism KW - Adenocarcinoma, Follicular -- genetics KW - Chromosome Aberrations KW - Thyroid Hormone Receptors beta -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68478155?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+genetics+and+cytogenetics&rft.atitle=Chromosomal+aberrations+in+cell+lines+derived+from+thyroid+tumors+spontaneously+developed+in+TRbetaPV%2FPV+mice.&rft.au=Zimonjic%2C+Drazen+B%3BKato%2C+Yasuhito%3BYing%2C+Hao%3BPopescu%2C+Nicholas+C%3BCheng%2C+Sheue-Yann&rft.aulast=Zimonjic&rft.aufirst=Drazen&rft.date=2005-09-01&rft.volume=161&rft.issue=2&rft.spage=104&rft.isbn=&rft.btitle=&rft.title=Cancer+genetics+and+cytogenetics&rft.issn=01654608&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-06 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted lipidomics: signaling lipids and drugs of abuse. AN - 68476258; 16099407 JF - Prostaglandins & other lipid mediators AU - Rapaka, Rao S AU - Piomelli, Daniele AU - Spiegel, Sarah AU - Bazan, Nicolas AU - Dennis, Edward A AD - Chemistry and Physiological Systems Research Branch, Division of Basic Neuroscience and Behavioral Research, National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD 20892, USA. r82u@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 223 EP - 234 VL - 77 IS - 1-4 SN - 1098-8823, 1098-8823 KW - Pharmaceutical Preparations KW - 0 KW - Index Medicus KW - Central Nervous System -- metabolism KW - Animals KW - Pharmaceutical Preparations -- metabolism KW - Humans KW - Brain -- metabolism KW - Models, Biological KW - Substance-Related Disorders -- therapy KW - Substance-Related Disorders -- metabolism KW - Lipid Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68476258?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prostaglandins+%26+other+lipid+mediators&rft.atitle=Targeted+lipidomics%3A+signaling+lipids+and+drugs+of+abuse.&rft.au=Rapaka%2C+Rao+S%3BPiomelli%2C+Daniele%3BSpiegel%2C+Sarah%3BBazan%2C+Nicolas%3BDennis%2C+Edward+A&rft.aulast=Rapaka&rft.aufirst=Rao&rft.date=2005-09-01&rft.volume=77&rft.issue=1-4&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=Prostaglandins+%26+other+lipid+mediators&rft.issn=10988823&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-15 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):1-3 [16099385] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted lipidomics and drug abuse research. AN - 68475216; 16099406 AB - Contemporaneously with genomics and proteomics technologies, lipidomics may be recognized as the next important emerging technology. The emergence of this important area of "omics" could very well mark the beginning of "the decade of the lipids." A workshop on lipidomics was held in Washington, DC, by the National Institute on Drug Abuse (NIDA) in April 2004. The goal of the workshop was to bring together scientists working at the frontier of lipid research, to discuss their findings in this area and to promote "lipidomics," in general, but also with a special focus on its application to drug abuse research and development of therapies to treat addiction. JF - Prostaglandins & other lipid mediators AU - Rapaka, Rao S AD - Chemistry & Physiological Systems Research Branch, Division of Basic Neuroscience & Behavioral Research, National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD 20892, USA. r82u@nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 219 EP - 222 VL - 77 IS - 1-4 SN - 1098-8823, 1098-8823 KW - Lipids KW - 0 KW - Index Medicus KW - Central Nervous System -- metabolism KW - Animals KW - Drug Industry -- trends KW - Humans KW - Models, Biological KW - Drug Design KW - Lipid Metabolism KW - Substance-Related Disorders -- therapy KW - Lipids -- chemistry KW - Substance-Related Disorders -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68475216?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Prostaglandins+%26+other+lipid+mediators&rft.atitle=Targeted+lipidomics+and+drug+abuse+research.&rft.au=Rapaka%2C+Rao+S&rft.aulast=Rapaka&rft.aufirst=Rao&rft.date=2005-09-01&rft.volume=77&rft.issue=1-4&rft.spage=219&rft.isbn=&rft.btitle=&rft.title=Prostaglandins+%26+other+lipid+mediators&rft.issn=10988823&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-15 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):1-3 [16099385] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thermal stability of hepatitis E virus. AN - 68459855; 16088844 AB - The thermal stability of virulent hepatitis E virus (HEV) and hepatitis A virus (HAV) was compared. Fecal suspensions of virus were heated to temperatures between 45 degrees C and 70 degrees C, and residual infectivity was determined in a cell culture system that was permissive for both viruses. Although HEV was less stable than was HAV, some HEV would most likely survive the internal temperatures of rare-cooked meat. JF - The Journal of infectious diseases AU - Emerson, Suzanne U AU - Arankalle, Vidya A AU - Purcell, Robert H AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8009, USA. semerson@niaid.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 930 EP - 933 VL - 192 IS - 5 SN - 0022-1899, 0022-1899 KW - Abridged Index Medicus KW - Index Medicus KW - Microscopy, Fluorescence KW - Hepatitis E -- transmission KW - Hot Temperature KW - Hepatitis A -- virology KW - Food Microbiology KW - Humans KW - Feces -- virology KW - Hepatitis A -- transmission KW - Hepatitis E -- virology KW - Cell Line KW - Hepatitis A virus -- growth & development KW - Hepatitis E virus -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68459855?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+infectious+diseases&rft.atitle=Thermal+stability+of+hepatitis+E+virus.&rft.au=Emerson%2C+Suzanne+U%3BArankalle%2C+Vidya+A%3BPurcell%2C+Robert+H&rft.aulast=Emerson&rft.aufirst=Suzanne&rft.date=2005-09-01&rft.volume=192&rft.issue=5&rft.spage=930&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+infectious+diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-08-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effect of tumor necrosis factor antagonism on allergen-mediated asthmatic airway inflammation. AN - 68455009; 16085220 AB - To assess whether tumor necrosis factor (TNF) antagonism can attenuate eosinophilic airway inflammation in patients with mild-to-moderate allergic asthma. Randomized, double-blind, placebo-controlled trial. National Institutes of Health (NIH) Clinical Center. Twenty-six patients with mild-to-moderate allergic asthma, receiving only inhaled beta-2-agonists, who demonstrated both an early and late phase response to inhalational allergen challenge. Injection of a soluble TNF receptor (TNFR:Fc, etanercept, Enbrel) or placebo, 25mg subcutaneously, twice weekly for 2 weeks, followed by a bronchoscopic segmental allergen challenge. The primary outcome measure was whether TNFR:Fc can access the lung and inhibit TNF bioactivity. Secondary outcome measures included pulmonary eosinophilia, Th2-type cytokines, and airway hyperresponsiveness. Anti-TNF therapy was associated with transient hemiplegia in one patient, which resulted in suspension of the study. Data from the 21 participants who completed the study were analyzed. Following treatment, patients receiving anti-TNF therapy had significantly increased TNFR2 levels in epithelial lining fluid (ELF) (P<0.001), consistent with delivery of TNFR:Fc to the lung. TNF antagonism did not attenuate pulmonary eosinophilia and was associated with an increase in ELF IL-4 levels (P=0.033) at 24h following segmental allergen challenge. TNF antagonism was not associated with a change in airway hyperresponsiveness to methacholine. TNF antagonism may not be effective for preventing allergen-mediated eosinophilic airway inflammation in mild-to-moderate asthmatics. Transient hemiplegia, which may mimic an evolving stroke, may be a potential toxicity of anti-TNF therapy. JF - Respiratory medicine AU - Rouhani, Farshid N AU - Meitin, Catherine A AU - Kaler, Maryann AU - Miskinis-Hilligoss, Dianne AU - Stylianou, Mario AU - Levine, Stewart J AD - Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 6D03, MSC 1590, Bethesda, MD 20892-1590, USA. Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 1175 EP - 1182 VL - 99 IS - 9 SN - 0954-6111, 0954-6111 KW - Allergens KW - 0 KW - Anti-Inflammatory Agents, Non-Steroidal KW - Cytokines KW - Immunoglobulin G KW - Receptors, Tumor Necrosis Factor KW - Tumor Necrosis Factor-alpha KW - Etanercept KW - OP401G7OJC KW - Index Medicus KW - Hemiplegia -- chemically induced KW - Anti-Inflammatory Agents, Non-Steroidal -- therapeutic use KW - Double-Blind Method KW - Cytokines -- biosynthesis KW - Humans KW - Leukocyte Count KW - Bronchial Hyperreactivity -- drug therapy KW - Airway Obstruction -- drug therapy KW - Anti-Inflammatory Agents, Non-Steroidal -- adverse effects KW - Adult KW - Middle Aged KW - Th2 Cells -- immunology KW - Bronchoalveolar Lavage Fluid -- cytology KW - Male KW - Female KW - Allergens -- immunology KW - Pulmonary Eosinophilia -- drug therapy KW - Immunoglobulin G -- adverse effects KW - Asthma -- drug therapy KW - Receptors, Tumor Necrosis Factor -- therapeutic use KW - Tumor Necrosis Factor-alpha -- antagonists & inhibitors KW - Immunoglobulin G -- therapeutic use KW - Asthma -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68455009?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Respiratory+medicine&rft.atitle=Effect+of+tumor+necrosis+factor+antagonism+on+allergen-mediated+asthmatic+airway+inflammation.&rft.au=Rouhani%2C+Farshid+N%3BMeitin%2C+Catherine+A%3BKaler%2C+Maryann%3BMiskinis-Hilligoss%2C+Dianne%3BStylianou%2C+Mario%3BLevine%2C+Stewart+J&rft.aulast=Rouhani&rft.aufirst=Farshid&rft.date=2005-09-01&rft.volume=99&rft.issue=9&rft.spage=1175&rft.isbn=&rft.btitle=&rft.title=Respiratory+medicine&rft.issn=09546111&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-07 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Localization of mesothelin in epithelial ovarian cancer. AN - 68450907; 16082249 AB - Mesothelin is a cell surface glycoprotein that is present on normal mesothelial cells and overexpressed in several cancers. On immunohistochemical examination of a limited number of ovarian tumors, increased mesothelin expression has been previously noted. The authors evaluated mesothelin expression in 48 patients with ovarian cancer who were screened for participation in phase 1 studies of a recombinant immunotoxin targeting mesothelin. Eligibility criteria for participation in the studies included mesothelin expression by more than 30% of accessible tumor cells. Sections of formalin-fixed paraffin-embedded tumor specimens were evaluated for mesothelin expression by immunohistochemistry using the anti-mesothelin monoclonal antibody K1. Between September 2000 and January 2003, 48 ovarian tumors were analyzed for mesothelin positivity. Mesothelin positivity was noted in 34 of the 48 cases evaluated (71%). These results show that mesothelin is expressed in most epithelial ovarian cancers and that mesothelin expression in ovarian cancers can be evaluated in archival material. Patients whose tumors express mesothelin could be eligible for participation in clinical trials of novel agents targeting mesothelin. JF - Applied immunohistochemistry & molecular morphology : AIMM AU - Hassan, Raffit AU - Kreitman, Robert J AU - Pastan, Ira AU - Willingham, Mark C AD - Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4264, USA. hassanr@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 243 EP - 247 VL - 13 IS - 3 SN - 1541-2016, 1541-2016 KW - Antibodies, Monoclonal KW - 0 KW - GPI-Linked Proteins KW - Immunotoxins KW - Membrane Glycoproteins KW - mesothelin KW - Index Medicus KW - Humans KW - Biopsy KW - Tissue Distribution KW - Patient Selection KW - Immunohistochemistry KW - Female KW - Antibodies, Monoclonal -- therapeutic use KW - Epithelial Cells -- pathology KW - Ovarian Neoplasms -- pathology KW - Membrane Glycoproteins -- analysis KW - Ovarian Neoplasms -- chemistry KW - Membrane Glycoproteins -- immunology KW - Ovarian Neoplasms -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68450907?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+immunohistochemistry+%26+molecular+morphology+%3A+AIMM&rft.atitle=Localization+of+mesothelin+in+epithelial+ovarian+cancer.&rft.au=Hassan%2C+Raffit%3BKreitman%2C+Robert+J%3BPastan%2C+Ira%3BWillingham%2C+Mark+C&rft.aulast=Hassan&rft.aufirst=Raffit&rft.date=2005-09-01&rft.volume=13&rft.issue=3&rft.spage=243&rft.isbn=&rft.btitle=&rft.title=Applied+immunohistochemistry+%26+molecular+morphology+%3A+AIMM&rft.issn=15412016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-10 N1 - Date created - 2005-08-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Introduction to the Special Section on Dissemination -- Dissemination Research and Research Dissemination: How can We Close the Gap? AN - 57166801; 200605137 AB - One of the greatest challenges facing health promotion & disease prevention is translating research findings into evidence-based public health & clinical practices that are actively disseminated & widely adopted. Despite the tremendous strides made in developing effective disease prevention & control programs, there has been little study of effective dissemination of evidence-based programs to & adoption by community, public health, & clinical practice settings. This special section provides a venue in which to highlight exemplary dissemination research efforts while also identifying limitations in research to date & framing important future research questions. This issue establishes a resource for investigators interested in dissemination research, with relevance to health psychology. In this sense, it can serve as a benchmark by which to examine subsequent progress. The 6 articles reflect the state of the science in dissemination research for the promotion & adoption of health behavior change interventions. 17 References. [Copyright 2005 The American Psychological Association.] JF - Health Psychology AU - Kerner, Jon AU - Rimer, Barbara AU - Emmons, Karen AD - Division Cancer Control & Population Sciences, National Cancer Instit, National Instits Health, Bethesda, MD kernerj@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 443 EP - 446 PB - American Psychological Association, Washington DC VL - 24 IS - 5 SN - 0278-6133, 0278-6133 KW - dissemination research, knowledge translation KW - Health psychology KW - Clinical practice KW - Research transfer KW - Public health KW - Dissemination KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57166801?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+Psychology&rft.atitle=Introduction+to+the+Special+Section+on+Dissemination+--+Dissemination+Research+and+Research+Dissemination%3A+How+can+We+Close+the+Gap%3F&rft.au=Kerner%2C+Jon%3BRimer%2C+Barbara%3BEmmons%2C+Karen&rft.aulast=Kerner&rft.aufirst=Jon&rft.date=2005-09-01&rft.volume=24&rft.issue=5&rft.spage=443&rft.isbn=&rft.btitle=&rft.title=Health+Psychology&rft.issn=02786133&rft_id=info:doi/10.1037%2F0278-6133.24.5.443 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-05-02 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Dissemination; Research transfer; Health psychology; Public health; Clinical practice DO - http://dx.doi.org/10.1037/0278-6133.24.5.443 ER - TY - JOUR T1 - Dental Visits among Smoking and Nonsmoking US Adults in 2000 AN - 57117950; 200602706 AB - Objective: To examine dental visits among smoking and nonsmoking adults in a nationally representative sample. Methods: Logistic regression analysis was performed, using a sample of 15,250 US adults from the Medical Expenditure Panel Survey Household Component 2000. Results: Current smokers were less likely to report dental visits (32.9%) than were nonsmokers (45.0%) during 2000. Differences were statistically significant even after accounting for other predictors of dental care use. Conclusions: Efforts to optimize the oral health of smokers and reduce serious oral diseases may benefit from addressing this lower use of dental services among smokers. 4 Tables, 23 References. Adapted from the source document. JF - American Journal of Health Behavior AU - Drilea, Susan K AU - Reid, Britt C AU - Li, Chien-Hsun AU - Hyman, Jeffrey J AU - Manski, Richard J AD - NIDCR/CDC Data Resource Center, Rockville, MD susan.drilea@ngc.com Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 462 EP - 471 VL - 29 IS - 5 SN - 1087-3244, 1087-3244 KW - dental visits, smoking, oral diseases, dental care, health education KW - Dental treatment KW - North American people KW - Dental care KW - Health education KW - Smokers KW - Nonsmokers KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57117950?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Health+Behavior&rft.atitle=Dental+Visits+among+Smoking+and+Nonsmoking+US+Adults+in+2000&rft.au=Drilea%2C+Susan+K%3BReid%2C+Britt+C%3BLi%2C+Chien-Hsun%3BHyman%2C+Jeffrey+J%3BManski%2C+Richard+J&rft.aulast=Drilea&rft.aufirst=Susan&rft.date=2005-09-01&rft.volume=48&rft.issue=3&rft.spage=839&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm049465g LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - AJHBF6 N1 - SubjectsTermNotLitGenreText - North American people; Smokers; Nonsmokers; Health education; Dental care; Dental treatment ER - TY - JOUR T1 - Response Reversal and Children with Psychopathic Tendencies: Success Is a Function of Salience of Contingency Change AN - 57092637; 200600111 AB - Background: Previous work has inconsistently reported difficulties with response reversal/extinction in children with psychopathic tendencies. Method: We tested the hypothesis that the degree of impairment seen in children with psychopathic tendencies is a function of the salience of contingency change. We investigated the performance of children with psychopathic tendencies on a novel probabilistic response reversal task involving four conditions with gradated reward-punishment contingencies (100-0, 90-10, 80-20 and 70-30; i.e., for the 100-0 contingency, responding to one object is always rewarded while responding to the other is always punished). Results: In line with predictions, the impairment seen in the children with psychopathic tendencies was an inverse function of the salience of the contingency change. Conclusions: We suggest that this data is consistent with suggestions of subtle orbital frontal cortex impairment in children with psychopathic tendencies. Illustrations, Tables, References. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Budhani, S AU - Blair, R J R AD - Unit Affective Cognitive Neuroscience, National Instit Mental Health, Bethesda, MD Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 972 EP - 981 VL - 46 IS - 9 SN - 0021-9630, 0021-9630 KW - ADHD KW - aggression KW - antisocial behavior KW - orbitofrontal cortex KW - psychopathy KW - response reversal KW - Mentally ill children KW - Psychopathy KW - Attention deficit hyperactivity disorder KW - Brain KW - Antisocial behaviour KW - Social aggression KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57092637?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Response+Reversal+and+Children+with+Psychopathic+Tendencies%3A+Success+Is+a+Function+of+Salience+of+Contingency+Change&rft.au=Budhani%2C+S%3BBlair%2C+R+J+R&rft.aulast=Budhani&rft.aufirst=S&rft.date=2005-09-01&rft.volume=46&rft.issue=9&rft.spage=972&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2004.00398.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Antisocial behaviour; Psychopathy; Mentally ill children; Attention deficit hyperactivity disorder; Social aggression; Brain DO - http://dx.doi.org/10.1111/j.1469-7610.2004.00398.x ER - TY - JOUR T1 - Hierarchical Linear Modeling Analyses of the NEO-PI-R Scales in the Baltimore Longitudinal Study of Aging AN - 57080490; 200619148 AB - The authors examined age trends in the 5 factors & 30 facets assessed by the Revised NEO Personality Inventory in Baltimore Longitudinal Study of Aging data (N = 1,944; 5,027 assessments) collected between 1989 & 2004. Consistent with cross-sectional results, hierarchical linear modeling analyses showed gradual personality changes in adulthood: a decline in Neuroticism up to age 80, stability & then decline in Extraversion, decline in Openness, increase in Agreeableness, & increase in Conscientiousness up to age 70. Some facets showed different curves from the factor they define. Birth cohort effects were modest, & there were no consistent Gender * Age interactions. Significant nonnormative changes were found for all 5 factors; they were not explained by attrition but might be due to genetic factors, disease, or life experience. Tables, Figures, Appendixes, References. [Copyright 2005 American Psychological Association.] JF - Psychology and Aging AU - Terracciano, Antonio AU - McCrae, Robert R AU - Brant, Larry J AU - Costa, Paul T, Jr AD - Gerontology Research Center, Baltimore, MD terraccianoa@grc.nia.nih.gov Y1 - 2005/09// PY - 2005 DA - September 2005 SP - 493 EP - 506 PB - American Psychological Association, Washington DC VL - 20 IS - 3 SN - 0882-7974, 0882-7974 KW - five-factor model, personality change, aging, longitudinal study, HLM KW - Ageing KW - Five factor model KW - Neuroticism KW - Personality KW - Extraversion KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57080490?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychology+and+Aging&rft.atitle=Hierarchical+Linear+Modeling+Analyses+of+the+NEO-PI-R+Scales+in+the+Baltimore+Longitudinal+Study+of+Aging&rft.au=Terracciano%2C+Antonio%3BMcCrae%2C+Robert+R%3BBrant%2C+Larry+J%3BCosta%2C+Paul+T%2C+Jr&rft.aulast=Terracciano&rft.aufirst=Antonio&rft.date=2005-09-01&rft.volume=48&rft.issue=3&rft.spage=764&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm0492709 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-11-29 N1 - Last updated - 2016-09-27 N1 - CODEN - PAGIEL N1 - SubjectsTermNotLitGenreText - Personality; Ageing; Neuroticism; Extraversion; Five factor model DO - http://dx.doi.org/10.1037/0882-7974.20.3.493 ER - TY - JOUR T1 - Personality profiles of cultures: aggregate personality traits AN - 38208558; 2987636 AB - Personality profiles of cultures can be operationalized as the mean trait levels of culture members. College students from 51 cultures rated an individual from their country whom they knew well (N = 12,156). Aggregate scores on Revised NEO Personality Inventory (NEO-PI-R) scales generalized across age and sex groups, approximated the individual-level 5-factor model, and correlated with aggregate self-report personality scores and other culture-level variables. Results were not attributable to national differences in economic development or to acquiescence. Geographical differences in scale variances and mean levels were replicated, with Europeans and Americans generally scoring higher in Extraversion than Asians and Africans. Findings support the rough scalar equivalence of NEO-PI-R factors and facets across cultures and suggest that aggregate personality profiles provide insight into cultural differences. Reprinted by permission of the American Psychological Association JF - Journal of personality and social psychology AU - McCrae, Robert R AU - Terracciano, Antonio AD - National Institutes of Health Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 407 EP - 425 VL - 89 IS - 3 SN - 0022-3514, 0022-3514 KW - Sociology KW - Social psychology KW - Personality traits KW - Cross-cultural analysis KW - Regression analysis KW - Statistical analysis KW - Cultural differences KW - Empirical research KW - Aggregate analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38208558?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+personality+and+social+psychology&rft.atitle=Personality+profiles+of+cultures%3A+aggregate+personality+traits&rft.au=McCrae%2C+Robert+R%3BTerracciano%2C+Antonio&rft.aulast=McCrae&rft.aufirst=Robert&rft.date=2005-09-01&rft.volume=89&rft.issue=3&rft.spage=407&rft.isbn=&rft.btitle=&rft.title=Journal+of+personality+and+social+psychology&rft.issn=00223514&rft_id=info:doi/10.1037%2F0022-3514.89.3.407 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 11901 10404; 9429 9416 2153; 3058 971; 4200 10902; 12224 971; 10739 12228 10919; 3121 3198 3549 2688 2449 10404; 658 971 DO - http://dx.doi.org/10.1037/0022-3514.89.3.407 ER - TY - JOUR T1 - Cocaine exposure is associated with subtle compromises of infants' and mothers' social emotional behavior and dyadic features of their interaction in the face-to-face still-face paradigm AN - 38196877; 2980357 AB - Prenatal cocaine and opiate exposure are thought to subtly compromise social and emotional development. The authors observed a large sample of 236 cocaine-exposed and 459 nonexposed infants (49 were opiate exposed and 646 nonexposed) with their mothers in the face-to-face still-face paradigm. Infant and maternal behaviors were microanalytically coded. No opiate-exposure effects were detected. However, mothers of cocaine-exposed infants showed more negative engagement than other mothers. The cocaine-exposed dyads also showed higher overall levels of mismatched engagement states than other dyads, including more negative engagement when the infants were in states of neutral engagement. Infants exposed to heavier levels of cocaine showed more passive-withdrawn negative engagement and engaged in more negative affective matching with their mothers than other infants. Although effect sizes were small, cocaine exposure, especially heavy cocaine exposure, was associated with subtly negative interchanges, which may have a cumulative impact on infants' later development and their relationships with their mothers. Reprinted by permission of the American Psychological Association JF - Developmental psychology AU - Tronick, E Z AU - Messinger, D S AU - Weinberg, M K AU - Lester, B M AU - LaGasse, L AU - Seifer, R AU - Bauer, C R AU - Shankaran, S AU - Bada, H AU - Wright, L L AU - Poole, K AU - Liu, J AD - Harvard University ; University of Miami ; Brown Medical School ; Wayne State University ; University of Tennessee ; National Institute of Child Health and Human Development ; Research Triangle Institute Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 711 EP - 722 VL - 41 IS - 5 SN - 0012-1649, 0012-1649 KW - Sociology KW - Emotions KW - Opiates KW - Personal contact KW - Communication KW - Engagement KW - Child development KW - Regression analysis KW - Personality development KW - Cocaine KW - Drugs KW - Developmental psychology KW - Motherhood KW - Infants UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38196877?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+psychology&rft.atitle=Cocaine+exposure+is+associated+with+subtle+compromises+of+infants%27+and+mothers%27+social+emotional+behavior+and+dyadic+features+of+their+interaction+in+the+face-to-face+still-face+paradigm&rft.au=Tronick%2C+E+Z%3BMessinger%2C+D+S%3BWeinberg%2C+M+K%3BLester%2C+B+M%3BLaGasse%2C+L%3BSeifer%2C+R%3BBauer%2C+C+R%3BShankaran%2C+S%3BBada%2C+H%3BWright%2C+L+L%3BPoole%2C+K%3BLiu%2C+J&rft.aulast=Tronick&rft.aufirst=E&rft.date=2005-09-01&rft.volume=41&rft.issue=5&rft.spage=711&rft.isbn=&rft.btitle=&rft.title=Developmental+psychology&rft.issn=00121649&rft_id=info:doi/10.1037%2F0012-1649.41.5.711 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 3518 10404; 3755; 2197 2212 6075 3483; 9407 6823; 9421 9416 2153; 2435 3755; 8953 3755; 10739 12228 10919; 4278; 6495 2212; 8316; 4196; 2572 DO - http://dx.doi.org/10.1037/0012-1649.41.5.711 ER - TY - JOUR T1 - Mothers' and fathers' behaviors toward their 3- to 4-month-old infants in lower, middle, and upper socioeconomic African American families AN - 38196613; 2980358 AB - African American mothers' and fathers' availability, caregiving, and social behaviors toward their infants in and around their homes were examined. Twenty lower, 21 middle, and 21 upper socioeconomic families and their 3- to 4-month-old infants were observed for 4 3-hr blocks between 8:00 a.m. and 8:00 p.m. on 4 different weekdays. With increasing economic resources, children's exposure to multiple caregivers and nonresident fathers declined. Mothers were more available to infants than fathers were, regardless of socioeconomic status. Mothers fed infants more than fathers did, whereas fathers vocalized more and displayed more affection to infants than mothers did when they were examined in proportion to caregiver presence. Mothers and fathers interacted with male and female infants quite similarly, although, in the upper socioeconomic families, fathers of daughters were more available than fathers of sons. Fathers and mothers in the different socioeconomic groups held, displayed affection to, and soothed their infants differently. Reprinted by permission of the American Psychological Association JF - Developmental psychology AU - Roopnarine, Jaipaul L AU - Fouts, Hillary N AU - Lamb, Michael E AU - Lewis-Elligan, Tracey Y AD - Syracuse University ; National Institute of Child Health and Human Development ; University of Cambridge ; DePaul University Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 723 EP - 732 VL - 41 IS - 5 SN - 0012-1649, 0012-1649 KW - Sociology KW - Socioeconomic status KW - Behavioural psychology KW - Gender differentiation KW - U.S.A. KW - Child care KW - Family studies KW - Fatherhood KW - African-Americans KW - Egalitarianism KW - Developmental psychology KW - Infants KW - Motherhood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38196613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+psychology&rft.atitle=Mothers%27+and+fathers%27+behaviors+toward+their+3-+to+4-month-old+infants+in+lower%2C+middle%2C+and+upper+socioeconomic+African+American+families&rft.au=Roopnarine%2C+Jaipaul+L%3BFouts%2C+Hillary+N%3BLamb%2C+Michael+E%3BLewis-Elligan%2C+Tracey+Y&rft.aulast=Roopnarine&rft.aufirst=Jaipaul&rft.date=2005-09-01&rft.volume=41&rft.issue=5&rft.spage=723&rft.isbn=&rft.btitle=&rft.title=Developmental+psychology&rft.issn=00121649&rft_id=info:doi/10.1037%2F0012-1649.41.5.723 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 3518 10404; 5423 3549 2688 2449 10404; 4783; 8316; 4827 9182; 11988 4011 3974 9390 11932 2328 11935 5837 2360 2688 2449 10404 11936; 2192; 4112 4368 9705 9713 6203; 1540 1543 10404; 635 1656 10555 6091 961 636 4424; 6495 2212; 433 293 14 DO - http://dx.doi.org/10.1037/0012-1649.41.5.723 ER - TY - JOUR T1 - delta -Aminolevulinic Acid Dehydratase Polymorphism and Risk of Brain Tumors in Adults AN - 20715995; 6524915 AB - The enzyme delta -aminolevulinic acid dehydratase (ALAD), which catalyzes the second step of heme synthesis, can be inhibited by several chemicals, including lead, a potential risk factor for brain tumors, particularly meningioma. In this study we examined whether the ALAD G177C polymorphism in the gene coding for ALAD is associated with risk of intracranial tumors of the brain and nervous system. We use data from a case-control study with 782 incident brain tumor cases and 799 controls frequency matched on hospital, age, sex, race/ethnicity, and residential proximity to the hospital. Blood samples were drawn and DNA subsequently sent for genotyping for 73% of subjects. ALAD genotype was determined for 94% of these samples (355 glioma, 151 meningioma, 67 acoustic neuroma, and 505 controls). Having one or more copy of the ALAD2 allele was associated with increased risk for meningioma [odds ratio (OR) = 1.6; 95% confidence interval (CI), 1.0-2.6], with the association appearing stronger in males (OR = 3.5; 95% CI, 1.3-9.2) than in females (OR = 1.2; 95% CI, 0.7-2.2). No increased risk associated with the ALAD2 variant was observed for glioma or acoustic neuroma. These findings suggest that the ALAD2 allele may increase genetic susceptibility to meningioma. JF - Environmental Health Perspectives AU - Rajaraman, P AU - Schwartz, B S AU - Rothman, N AU - Yeager, M AU - Fine, HA AU - Shapiro, W R AU - Selker, R G AU - Black, P M AU - Inskip, P D AD - National Cancer Institute, NIH, DHHS, 6120 Executive Blvd., EPS Room 7085, Bethesda, MD 20892-7238, USA, rajarama@mail.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1209 EP - 1211 VL - 113 IS - 9 SN - 0091-6765, 0091-6765 KW - Risk Abstracts; CSA Neurosciences Abstracts KW - Chemicals KW - Age KW - Heme KW - Gene polymorphism KW - tumors KW - Aminolevulinic acid KW - Genotypes KW - Lead KW - Neoplasia KW - glioma KW - Nervous system KW - Risk factors KW - Glioma KW - brain tumors KW - Races KW - Ethnic groups KW - Sex KW - Coding KW - Data processing KW - Genotyping KW - Brain KW - Enzymes KW - Brain tumors KW - DNA KW - meningioma KW - Hospitals KW - N3 11028:Neuropharmacology & toxicology KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20715995?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=delta+-Aminolevulinic+Acid+Dehydratase+Polymorphism+and+Risk+of+Brain+Tumors+in+Adults&rft.au=Rajaraman%2C+P%3BSchwartz%2C+B+S%3BRothman%2C+N%3BYeager%2C+M%3BFine%2C+HA%3BShapiro%2C+W+R%3BSelker%2C+R+G%3BBlack%2C+P+M%3BInskip%2C+P+D&rft.aulast=Rajaraman&rft.aufirst=P&rft.date=2005-09-01&rft.volume=113&rft.issue=9&rft.spage=1209&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7986 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Coding; Data processing; Heme; Genotyping; Gene polymorphism; Enzymes; Aminolevulinic acid; Neoplasia; Lead; Brain tumors; Nervous system; Risk factors; Glioma; meningioma; Ethnic groups; Races; Sex; Hospitals; Chemicals; glioma; Age; DNA; Brain; tumors; Genotypes; brain tumors DO - http://dx.doi.org/10.1289/ehp.7986 ER - TY - JOUR T1 - Reconstruction of the Conserved beta -Bulge in Mammalian Defensins Using D-Amino Acids AN - 20099445; 6527775 AB - Defensins are cationic antimicrobial mini-proteins that play important roles in the innate immune defense against microbial infection. Six invariant Cys residues in each defensin form three structurally indispensable intramolecular disulfide bridges. The only other residue invariant in all known mammalian defensins is a Gly. Structural studies indicate that the invariant Gly residue is located in an atypical, classic-type beta -bulge with the backbone torsion angles ({phi}, psi ) disallowed for L-amino acids but permissible for D-enantiomers. We replaced the invariant Gly super(17) residue in human neutrophil alpha -defensin 2 (HNP2) by L-Ala or one of the D-amino acids Ala, Glu, Phe, Arg, Thr, Val, or Tyr. Although L-Ala super(17)-HNP2 could not be folded, resulting in massive aggregation, all of the D-amino acid-substituted analogs folded with high efficiency. The high resolution x-ray crystal structures of dimeric D-Ala super(17)-HNP2 were determined in three different crystal forms, showing a well preserved beta -bulge identical to those found in other defensins. The seven D-analogs of HNP2 exhibited highly variable bactericidal activity against Gram-positive and Gram-negative test strains, consistent with the premise that interplay between charge and hydrophobicity dictates how amphiphilic defensins kill. Further, the bactericidal activity of these D-amino acid analogs of HNP2 correlated well with their ability to induce leakage from large unilamellar vesicles, supporting membrane permeabilization as the lethal event in microbial killing by HNP2. Our findings identify a conformational prerequisite in the beta -bulge of defensins essential for correct folding and native structure, thereby explaining the molecular basis of the Gly-Xaa-Cys motif conserved in all mammalian defensins. JF - Journal of Biological Chemistry AU - Xie, Cao AU - Prahl, Adam AU - Ericksen, Bryan AU - Wu, Zhibin AU - Zeng, Pengyun AU - Li, Xiangqun AU - Lu, Wei-Yue AU - Lubkowski, Jacek AU - Lu, Wuyuan AD - Institute of Human Virology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, Macromolecular Assembly Structure and Cell Signaling Section, NCI, National Institutes of Health, Frederick, Maryland 21702, and Fudan-PharmCo Drug Target Research Center, School of Pharmacy, Fudan University, Shanghai 200032, China Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 32921 EP - 32929 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 38 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology KW - Leakage KW - Leukocytes (neutrophilic) KW - Hydrophobicity KW - Crystals KW - Infection KW - Antimicrobial agents KW - Defensins KW - Ionizing radiation KW - Membrane vesicles KW - Crystal structure KW - D-Amino acids KW - Bactericidal activity KW - A 01340:Antibiotics & Antimicrobials KW - J 02340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20099445?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=In+the+quest+for+stable+rescuing+mutants+of+p53%3A+computational+mutagenesis+of+flexible+loop+L1.&rft.au=Pan%2C+Yongping%3BMa%2C+Buyong%3BVenkataraghavan%2C+R+Babu%3BLevine%2C+Arnold+J%3BNussinov%2C+Ruth&rft.aulast=Pan&rft.aufirst=Yongping&rft.date=2005-02-08&rft.volume=44&rft.issue=5&rft.spage=1423&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Defensins; Leakage; Ionizing radiation; Leukocytes (neutrophilic); Crystal structure; Membrane vesicles; Hydrophobicity; D-Amino acids; Crystals; Infection; Bactericidal activity; Antimicrobial agents ER - TY - JOUR T1 - A Decline in C sub(6) Antibody Titer Occurs in Successfully Treated Patients with Culture-Confirmed Early Localized or Early Disseminated Lyme Borreliosis AN - 20097863; 6529921 AB - C sub(6), a Borrelia burgdorferi-derived peptide, is used as the antigen in the C sub(6)-Lyme disease diagnostic test. We assessed retrospectively whether a fourfold decrease or a decrease to a negative value in anti-C sub(6) antibody titer is positively correlated with a positive response to treatment in a sample of culture-confirmed patients with either early localized (single erythema migrans [EM]; n = 93) or early disseminated (multiple EM; n = 27) disease. All of these patients had been treated with antibiotics and were free of disease within 6 to 12 months of follow-up. Results show that a serum specimen taken at this time was either C sub(6) negative or had a greater than or equal to 4-fold decrease in C sub(6) antibody titer with respect to a specimen taken at baseline (or during the early convalescent period if the baseline specimen was C sub(6) negative) for all of the multiple-EM patients (P < 0.0001) and in 89% of the single-EM patients (P < 0.0001). These results indicate that a decline in anti-C sub(6) antibody titer coincides with effective antimicrobial therapy in patients with early localized or early disseminated Lyme borreliosis. JF - Clinical and Diagnostic Laboratory Immunology AU - Philipp, Mario T AU - Wormser, Gary P AU - Marques, Adriana R AU - Bittker, Susan AU - Martin, Dale S AU - Nowakowski, John AU - Dally, Leonard G AD - Division of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana. Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla, New York. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland. The EMMES Corporation, Rockville, Maryland Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1069 EP - 1074 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 12 IS - 9 SN - 1071-412X, 1071-412X KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Antibodies KW - Erythema KW - Borrelia burgdorferi KW - Borreliosis KW - Borrelia KW - Antibiotics KW - Lyme disease KW - Antimicrobial agents KW - A 01340:Antibiotics & Antimicrobials KW - J 02833:Immune response and immune mechanisms KW - F 06106:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20097863?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+and+Diagnostic+Laboratory+Immunology&rft.atitle=A+Decline+in+C+sub%286%29+Antibody+Titer+Occurs+in+Successfully+Treated+Patients+with+Culture-Confirmed+Early+Localized+or+Early+Disseminated+Lyme+Borreliosis&rft.au=Philipp%2C+Mario+T%3BWormser%2C+Gary+P%3BMarques%2C+Adriana+R%3BBittker%2C+Susan%3BMartin%2C+Dale+S%3BNowakowski%2C+John%3BDally%2C+Leonard+G&rft.aulast=Philipp&rft.aufirst=Mario&rft.date=2005-09-01&rft.volume=12&rft.issue=9&rft.spage=1069&rft.isbn=&rft.btitle=&rft.title=Clinical+and+Diagnostic+Laboratory+Immunology&rft.issn=1071412X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Erythema; Antibodies; Borreliosis; Antibiotics; Antimicrobial agents; Lyme disease; Borrelia burgdorferi; Borrelia ER - TY - JOUR T1 - A Combined Proteome and Microarray Investigation of Inorganic Phosphate-induced Pre-osteoblast Cells AN - 20087560; 6530163 AB - Inorganic phosphate, which is generated during osteoblast differentiation and mineralization, has recently been identified as an important signaling molecule capable of altering signal transduction pathways and gene expression. A large scale quantitative proteomic investigation of pre-osteoblasts stimulated with inorganic phosphate for 24 h resulted in the identification of 2501 proteins, of which 410 (16%) had an altered abundance ratio of greater than or equal to 1.75-fold, either up or down, revealing both novel and previously defined osteoblast-regulated proteins. A pathway/function analysis of these proteins revealed an increase in cell cycle and proliferation that was subsequently verified by conventional biochemical means. To further analyze the mechanisms by which inorganic phosphate regulates cellular protein levels, we undertook a mRNA microarray analysis of pre-osteoblast cells at 18, 21, and 24 h after inorganic phosphate exposure. Comparison of the mRNA microarray data with the 24-hour quantitative proteomic data resulted in a generally weak overall correlation; the 21-hour RNA sample showed the highest correlation to the proteomic data. However, an analysis of osteoblast relevant proteins revealed a much higher correlation at all time points. A comparison of the microarray and proteomic datasets allowed for the identification of a number of candidate proteins that are post-transcriptionally regulated by elevated inorganic phosphate, including Fra-1, a member of the activator protein-1 family of transcription factors. The analysis of the data presented here not only sheds new light on the important roles of inorganic phosphate in osteoblast function but also begins to address the contribution of post-transcriptional and post-translational regulation to a cell's expressed proteome. The ability to accurately measure changes in both protein abundance and mRNA levels on a system-wide scale represents a novel means to extract data from previously one-dimensional datasets. JF - Molecular and Cellular Proteomics AU - Conrads, Kelly A AU - Yi, Ming AU - Simpson, Kerri A AU - Lucas, David A AU - Camalier, Corinne E AU - Yu, Li-Rong AU - Veenstra, Timothy D AU - Stephens, Robert M AU - Conrads, Thomas P AU - Beck, George RJr AD - Laboratory of Cancer Prevention, Center for Cancer Research, Advanced Biomedical Computing Center, SAIC-Frederick, Inc., and Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., National Cancer Institute, Frederick, Maryland 21702 Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1284 EP - 1296 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.asbmb.org/] VL - 4 IS - 9 SN - 1535-9476, 1535-9476 KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Osteoblastogenesis KW - Fra1 protein KW - Data processing KW - Cell cycle KW - Activator protein 1 KW - Mineralization KW - DNA microarrays KW - Gene expression KW - Osteoblasts KW - Post-translation KW - Phosphate KW - Transcription factors KW - proteomics KW - Post-transcription KW - Signal transduction KW - N 14045:Transcriptional regulation KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20087560?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+Cellular+Proteomics&rft.atitle=A+Combined+Proteome+and+Microarray+Investigation+of+Inorganic+Phosphate-induced+Pre-osteoblast+Cells&rft.au=Conrads%2C+Kelly+A%3BYi%2C+Ming%3BSimpson%2C+Kerri+A%3BLucas%2C+David+A%3BCamalier%2C+Corinne+E%3BYu%2C+Li-Rong%3BVeenstra%2C+Timothy+D%3BStephens%2C+Robert+M%3BConrads%2C+Thomas+P%3BBeck%2C+George+RJr&rft.aulast=Conrads&rft.aufirst=Kelly&rft.date=2005-09-01&rft.volume=4&rft.issue=9&rft.spage=1284&rft.isbn=&rft.btitle=&rft.title=Molecular+and+Cellular+Proteomics&rft.issn=15359476&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Osteoblastogenesis; Fra1 protein; Data processing; Activator protein 1; Cell cycle; Mineralization; DNA microarrays; Gene expression; Osteoblasts; Phosphate; Post-translation; Transcription factors; proteomics; Post-transcription; Signal transduction ER - TY - JOUR T1 - Single nucleotide polymorphism in transcriptional regulatory regions and expression of environmentally responsive genes AN - 20022293; 8252197 AB - Single nucleotide polymorphisms (SNPs) in the human genome are DNA sequence variations that can alter an individual's response to environmental exposure. SNPs in gene coding regions can lead to changes in the biological properties of the encoded protein. In contrast, SNPs in non-coding gene regulatory regions may affect gene expression levels in an allele-specific manner, and these functional polymorphisms represent an important but relatively unexplored class of genetic variation. The main challenge in analyzing these SNPs is a lack of robust computational and experimental methods. Here, we first outline mechanisms by which genetic variation can impact gene regulation, and review recent findings in this area; then, we describe a methodology for bioinformatic discovery and functional analysis of regulatory SNPs in cis-regulatory regions using the assembled human genome sequence and databases on sequence polymorphism and gene expression. Our method integrates SNP and gene databases and uses a set of computer programs that allow us to: (1) select SNPs, from among the >9 million human SNPs in the NCBI dbSNP database, that are similar to cis-regulatory element (RE) consensus sequences; (2) map the selected dbSNP entries to the human genome assembly in order to identify polymorphic REs near gene start sites; (3) prioritize the candidate polymorphic RE containing genes by searching the existing genotype and gene expression data sets. The applicability of this system has been demonstrated through studies on p53 responsive elements and is being extended to additional pathways and environmentally responsive genes. . JF - Toxicology and Applied Pharmacology AU - Wang, X AU - Tomso, D J AU - Liu, X AU - Bell, DA AD - Laboratory of Computational Biology and Risk Analysis, National Institute of Environmental Health Sciences, Mail Drop: C3-03, PO Box 12233, Research Triangle Park, NC 27709, USA, BELL1@niehs.nih.gov Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 84 EP - 90 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 207 IS - 2 SN - 0041-008X, 0041-008X KW - Genetics Abstracts; Toxicology Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Genomes KW - Data processing KW - Gene polymorphism KW - Nucleotide sequence KW - Regulatory sequences KW - Genetic diversity KW - Transcription KW - Computer applications KW - p53 protein KW - Computer programs KW - Databases KW - Single-nucleotide polymorphism KW - Reviews KW - Gene regulation KW - Bioinformatics KW - X 24310:Pharmaceuticals KW - N 14815:Nucleotide Sequence KW - G 07780:Fungi UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20022293?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Single+nucleotide+polymorphism+in+transcriptional+regulatory+regions+and+expression+of+environmentally+responsive+genes&rft.au=Wang%2C+X%3BTomso%2C+D+J%3BLiu%2C+X%3BBell%2C+DA&rft.aulast=Wang&rft.aufirst=X&rft.date=2005-09-01&rft.volume=207&rft.issue=2&rft.spage=84&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.09.024 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Genomes; Data processing; Regulatory sequences; Nucleotide sequence; Gene polymorphism; Transcription; Genetic diversity; Computer applications; p53 protein; Databases; Computer programs; Single-nucleotide polymorphism; Gene regulation; Reviews; Bioinformatics DO - http://dx.doi.org/10.1016/j.taap.2004.09.024 ER - TY - JOUR T1 - Serotonergic-like progenitor cells propagated from neural stem cells in vitro: survival with SERT protein expression following implantation into brains of mice lacking SERT AN - 19975698; 7327526 AB - During mammalian brain development serotonin (5-HT) contributes to neuronal proliferation, migration and differentiation in cortex and other brain regions. In adulthood the brain serotonergic system thus participates in the coordination of complex sensory and motor functions that are associated with different behavioral states and are implicated in multiple neuropsychiatric disorders. The aim of the present study was to propagate serotonergic-like progenitor cells from mouse neural stem cells, to evaluate their properties [e.g., serotonin transporter (SERT)-mediated reuptake activity and receptor-mediated electrophysiological responses], then to implant these progenitor cells into the brains of mice with a targeted disruption of SERT, to investigate whether these implanted progenitors would survive, maintain the same phenotype and express SERT in the adult brain of SERT knockout mice. JF - FASEB Journal AU - Ren-Patterson, R F AU - Kim, D-K AU - Zheng, X AU - Sherrill, S AU - Huang, S-J AU - Tolliver, T AU - Murphy, D L AD - Laboratory of Clinical Science, National Institute of Mental Health, Building 10, Room 3D41, Bethesda, MD 20892-1264, USA, renpatr@intra.nimh.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1537 EP - 1539 VL - 19 IS - 11 SN - 0892-6638, 0892-6638 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Cell survival KW - Differentiation KW - Cortex (motor) KW - Mental disorders KW - Cortex KW - Brain KW - Cell migration KW - Serotonin transporter KW - Neural stem cells KW - Serotonin KW - Cortex (somatosensory) KW - N3 11003:Developmental neuroscience KW - W 30955:Biosensors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19975698?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+Journal&rft.atitle=Serotonergic-like+progenitor+cells+propagated+from+neural+stem+cells+in+vitro%3A+survival+with+SERT+protein+expression+following+implantation+into+brains+of+mice+lacking+SERT&rft.au=Ren-Patterson%2C+R+F%3BKim%2C+D-K%3BZheng%2C+X%3BSherrill%2C+S%3BHuang%2C+S-J%3BTolliver%2C+T%3BMurphy%2C+D+L&rft.aulast=Ren-Patterson&rft.aufirst=R&rft.date=2005-09-01&rft.volume=19&rft.issue=11&rft.spage=1537&rft.isbn=&rft.btitle=&rft.title=FASEB+Journal&rft.issn=08926638&rft_id=info:doi/10.1096%2Ffj.04-3657fje LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Cell survival; Differentiation; Mental disorders; Cortex (motor); Cortex; Brain; Cell migration; Serotonin transporter; Neural stem cells; Cortex (somatosensory); Serotonin DO - http://dx.doi.org/10.1096/fj.04-3657fje ER - TY - JOUR T1 - Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray AN - 19944806; 6518231 AB - The possibility of generating neural cells from human bone-marrow-derived mesenchymal stem cells (hMSCs) by simple in vitro treatments is appealing both conceptually and practically. However, whether phenotypic modulations observed after chemical manipulation of such stem cells truly represent a genuine trans-lineage differentiation remains to be established. We have re-evaluated the effects of a frequently reported biochemical approach, based on treatment with butylated hydroxyanisole and dimethylsulphoxide, to bring about such phenotypic conversion by monitoring the morphological changes induced by the treatment in real time, by analysing the expression of phenotype-specific protein markers and by assessing the modulation of transcriptome. Video time-lapse microscopy showed that conversion of mesenchymal stem cells to a neuron-like morphology could be reproduced in normal primary fibroblasts as well as mimicked by addition of drugs eliciting cytoskeletal collapse and disruption of focal adhesion contacts. Analysis of markers revealed that mesenchymal stem cells constitutively expressed multi-lineage traits, including several pertaining to the neural one. However, the applied `neural induction' protocol neither significantly modulated the expression of such markers, nor induced de novo translation of other neural-specific proteins. Similarly, global expression profiling of over 21,000 genes demonstrated that gene transcription was poorly affected. Most strikingly, we found that the set of genes whose expression was altered by the inductive treatment did not match those sets of genes differentially expressed when comparing untreated mesenchymal stem cells and immature neural tissues. Conversely, by comparing these gene expression profiles with that obtained from comparisons between the same cells and an unrelated non-neural organ, such as liver, we found that the adopted neural induction protocol was no more effective in redirecting human mesenchymal stem cells toward a neural phenotype than toward an endodermal hepatic pathway. JF - Journal of Cell Science AU - Bertani, Nicoletta AU - Malatesta, Paolo AU - Volpi, Giorgia AU - Sonego, Paolo AU - Perris, Roberto AD - Department of Evolutionary and Functional Biology, University of Parma, Viale delle Scienze 11/a, 43100 Parma, Italy. Division for Experimental Oncology 2, The National Cancer Institute, CRO-IRCCS, Via Pedemontana Occidentale 1, Aviano 33081, Italy Y1 - 2005/09/01/ PY - 2005 DA - 2005 Sep 01 SP - 3925 EP - 3936 PB - Company of Biologists, 140 Cowley Road Cambridge CB4 0DL UK, [URL:http://www.biologists.com/web/index.html] VL - 118 IS - 17 SN - 0021-9533, 0021-9533 KW - focal adhesion KW - man KW - Biotechnology and Bioengineering Abstracts; Genetics Abstracts; CSA Neurosciences Abstracts KW - Translation KW - Transcription KW - DNA microarrays KW - Cell adhesion KW - Fibroblasts KW - Cytoskeleton KW - Gene expression KW - Differentiation KW - Neurogenesis KW - Neuromodulation KW - Stem cells KW - Butylated hydroxyanisole KW - Microscopy KW - Dimethyl sulfoxide KW - Liver KW - Cell fate KW - Mesenchyme KW - Drugs KW - G 07433:Miscellaneous KW - N3 11070:Neurochemistry and cellular biology KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19944806?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Cell+Science&rft.atitle=Neurogenic+potential+of+human+mesenchymal+stem+cells+revisited%3A+analysis+by+immunostaining%2C+time-lapse+video+and+microarray&rft.au=Bertani%2C+Nicoletta%3BMalatesta%2C+Paolo%3BVolpi%2C+Giorgia%3BSonego%2C+Paolo%3BPerris%2C+Roberto&rft.aulast=Bertani&rft.aufirst=Nicoletta&rft.date=2005-09-01&rft.volume=118&rft.issue=17&rft.spage=3925&rft.isbn=&rft.btitle=&rft.title=Journal+of+Cell+Science&rft.issn=00219533&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-06-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Translation; Transcription; DNA microarrays; Fibroblasts; Cell adhesion; Gene expression; Cytoskeleton; Differentiation; Stem cells; Neuromodulation; Neurogenesis; Butylated hydroxyanisole; Microscopy; Liver; Dimethyl sulfoxide; Cell fate; Mesenchyme; Drugs ER - TY - JOUR T1 - Retroviral Integration Sites Correlate with Expressed Genes in Hematopoietic Stem Cells AN - 19935335; 6529896 AB - In this study, we analyzed whether retroviral integration sites in repopulating hematopoietic cells correlate with genes expressed in fractions enriched in hematopoietic stem cells (HSCs). We have previously described microarray studies of two populations enriched in HSCs: CD34 super(+)/CD38 super(-) and the slow dividing fraction of CD34 super(+)/CD38 super(-) cells (SDF). Furthermore, we demonstrated that oncoretroviral integrations in severe combined immunodeficient repopulating cells are preferentially located near the transcription start. Here, we have identified 117 corresponding cDNA clones on our micro-array representing genes with retroviral integration sites. These genes revealed a higher mean signal intensity in comparison with either all genes on the array or a subset of control genes with retroviral integrations in HeLa cells. Furthermore, these genes demonstrated a higher expression in CD34 super(+)/CD38 super(-) cells and SDF. The association of gene expression and retrovirally targeted genes observed here will help to elucidate the molecular characteristics of primitive repopulating hematopoietic cells. JF - Stem Cells AU - Wagner, Wolfgang AU - Laufs, Stephanie AU - Blake, Jonathon AU - Schwager, Christian AU - Wu, Xiaolin AU - Zeller, Jens W AU - Ho, Anthony D AU - Fruehauf, Stefan AD - Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany. German Cancer Research Center (DKFZ), Heidelberg, Germany. Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany. Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 1050 EP - 1058 PB - AlphaMed Press, Inc., One Prestige Pl, Ste 290 Miamisburg OH 45342-3758 USA VL - 23 IS - 8 SN - 1066-5099, 1066-5099 KW - Genetics Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Expression vectors KW - Gene expression KW - Integration KW - Stem cells KW - Gene therapy KW - Immunodeficiency KW - Hemopoiesis KW - Transcription KW - Population studies KW - CD34 antigen KW - DNA microarrays KW - G 07720:Immunogenetics KW - W3 33181:Gene therapy vectors KW - W 30965:Miscellaneous, Reviews KW - V 22310:Genetics, Taxonomy & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19935335?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Stem+Cells&rft.atitle=Retroviral+Integration+Sites+Correlate+with+Expressed+Genes+in+Hematopoietic+Stem+Cells&rft.au=Wagner%2C+Wolfgang%3BLaufs%2C+Stephanie%3BBlake%2C+Jonathon%3BSchwager%2C+Christian%3BWu%2C+Xiaolin%3BZeller%2C+Jens+W%3BHo%2C+Anthony+D%3BFruehauf%2C+Stefan&rft.aulast=Wagner&rft.aufirst=Wolfgang&rft.date=2005-09-01&rft.volume=23&rft.issue=8&rft.spage=1050&rft.isbn=&rft.btitle=&rft.title=Stem+Cells&rft.issn=10665099&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Gene expression; Expression vectors; Integration; Stem cells; Gene therapy; Immunodeficiency; Population studies; Transcription; Hemopoiesis; CD34 antigen; DNA microarrays ER - TY - JOUR T1 - Green Tea Epigallocatechin-3-Gallate (EGCG) Modulates Amyloid Precursor Protein Cleavage and Reduces Cerebral Amyloidosis in Alzheimer Transgenic Mice AN - 19833184; 6529532 AB - Alzheimer's disease (AD) is a progressive neurodegenerative disorder pathologically characterized by deposition of beta -amyloid (A beta ) peptides as senile plaques in the brain. Recent studies suggest that green tea flavonoids may be used for the prevention and treatment of a variety of neurodegenerative diseases. Here, we report that (-)-epigallocatechin-3-gallate (EGCG), the main polyphenolic constituent of green tea, reduces A beta generation in both murine neuron-like cells (N2a) transfected with the human "Swedish" mutant amyloid precursor protein (APP) and in primary neurons derived from Swedish mutant APP-overexpressing mice (Tg APP sub(sw) line 2576). In concert with these observations, we find that EGCG markedly promotes cleavage of the alpha -C-terminal fragment of APP and elevates the N-terminal APP cleavage product, soluble APP- alpha . These cleavage events are associated with elevated alpha -secretase activity and enhanced hydrolysis of tumor necrosis factor alpha -converting enzyme, a primary candidate alpha -secretase. As a validation of these findings in vivo, we treated Tg APP sub(sw) transgenic mice overproducing A beta with EGCG and found decreased A beta levels and plaques associated with promotion of the nonamyloidogenic alpha -secretase proteolytic pathway. These data raise the possibility that EGCG dietary supplementation may provide effective prophylaxis for AD. JF - Journal of Neuroscience AU - Rezai-Zadeh, Kavon AU - Shytle, Doug AU - Sun, Nan AU - Mori, Takashi AU - Hou, Huayan AU - Jeanniton, Deborah AU - Ehrhart, Jared AU - Townsend, Kirk AU - Zeng, Jin AU - Morgan, David AU - Hardy, John AU - Town, Terrence AU - Tan, Jun AD - Neuroimmunology Laboratory, Silver Child Development Center, Department of Psychiatry and Behavioral Medicine, Center for Excellence in Aging and Brain Repair, Department of Neurosurgery, Alzheimer's Disease Research Laboratory, Department of Pharmacology, University of South Florida, Tampa, Florida 33613, Institute of Medical Science, Saitama Medical School, Saitama 350-8550, Japan, Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, and Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06519 Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 8807 EP - 8814 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 38 SN - 0270-6474, 0270-6474 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Proteolysis KW - epigallocatechin-3-gallate KW - Flavonoids KW - Data processing KW - green tea KW - Tumor necrosis factor KW - Alzheimer's disease KW - Brain KW - Enzymes KW - Transgenic mice KW - Hydrolysis KW - Amyloid precursor protein KW - Neurodegenerative diseases KW - Nervous system KW - Amyloidosis KW - Dietary supplements KW - Neurons KW - Prophylaxis KW - Secretase KW - beta -Amyloid KW - Senile plaques KW - N3 11126:Alzheimer's disease and other dementias KW - W 30935:Food Biotechnology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19833184?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Green+Tea+Epigallocatechin-3-Gallate+%28EGCG%29+Modulates+Amyloid+Precursor+Protein+Cleavage+and+Reduces+Cerebral+Amyloidosis+in+Alzheimer+Transgenic+Mice&rft.au=Rezai-Zadeh%2C+Kavon%3BShytle%2C+Doug%3BSun%2C+Nan%3BMori%2C+Takashi%3BHou%2C+Huayan%3BJeanniton%2C+Deborah%3BEhrhart%2C+Jared%3BTownsend%2C+Kirk%3BZeng%2C+Jin%3BMorgan%2C+David%3BHardy%2C+John%3BTown%2C+Terrence%3BTan%2C+Jun&rft.aulast=Rezai-Zadeh&rft.aufirst=Kavon&rft.date=2005-09-01&rft.volume=25&rft.issue=38&rft.spage=8807&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Proteolysis; epigallocatechin-3-gallate; Flavonoids; Data processing; green tea; Tumor necrosis factor; Alzheimer's disease; Brain; Enzymes; Transgenic mice; Hydrolysis; Amyloid precursor protein; Neurodegenerative diseases; Amyloidosis; Nervous system; Neurons; Dietary supplements; Prophylaxis; beta -Amyloid; Secretase; Senile plaques ER - TY - JOUR T1 - Expression and Targeting of Interleukin-4 Receptor for Primary and Advanced Ovarian Cancer Therapy AN - 19829078; 6527213 AB - Because the most characteristic property of ovarian cancer is i.p. spread, the majority of patients are diagnosed at an advanced stage, leading to limited availability of options for curative therapies. With an intent to identify targeted therapeutic approaches, we have observed that similar to 60% of 21 ovarian cancer tissue samples express a high density of interleukin-4 receptor (IL-4R), whereas normal ovarian tissues tested (n = 7) expressed no or low levels of IL-4R. To target IL-4R, we have developed IL-4 cytotoxin, in which circular-permuted IL-4 is fused to a mutated form of Pseudomonas exotoxin. This cytotoxin is specifically and highly cytotoxic to PA-1, IGROV-1, and SK-OV3 ovarian carcinoma cell lines in vitro. In addition, it shows remarkable antitumor activities against established s.c. ovarian tumors in immunodeficient animals. i.p. administration of IL-4 cytotoxin in mice with orthotopically implanted ovarian tumors caused regression of established tumors and prevented these animals from tumor metastasis. Continuous i.p. infusion of IL-4 cytotoxin prolonged survival of tumor-bearing mice even with bulky disease. These results indicate that IL-4R-targeted cytotoxin may be a useful agent for the management of patients with ovarian cancer, and further studies need to be done to evaluate its safety, tolerability, and efficacy. JF - Cancer Research AU - Kioi, Mitomu AU - Takahashi, Satoru AU - Kawakami, Mariko AU - Kawakami, Koji AU - Kreitman, Robert J AU - Puri, Raj K AD - Laboratory of Molecular Tumor Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biosystems and Cancer, Center for Cancer Research, and Laboratory of Molecular Biology, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 8388 EP - 8396 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 18 SN - 0008-5472, 0008-5472 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Interleukin 4 KW - Immunotherapy KW - Cytotoxins KW - Immunodeficiency KW - Pseudomonas KW - Tumors KW - Interleukin 4 receptors KW - Exotoxins KW - Metastases KW - Cytotoxicity KW - Tumor cell lines KW - Receptor density KW - Ovarian carcinoma KW - Antitumor activity KW - F 06152:Tumor Immunology KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19829078?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=Expression+and+Targeting+of+Interleukin-4+Receptor+for+Primary+and+Advanced+Ovarian+Cancer+Therapy&rft.au=Kioi%2C+Mitomu%3BTakahashi%2C+Satoru%3BKawakami%2C+Mariko%3BKawakami%2C+Koji%3BKreitman%2C+Robert+J%3BPuri%2C+Raj+K&rft.aulast=Kioi&rft.aufirst=Mitomu&rft.date=2005-09-01&rft.volume=65&rft.issue=18&rft.spage=8388&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-06-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Interleukin 4; Cytotoxins; Immunotherapy; Immunodeficiency; Tumors; Interleukin 4 receptors; Exotoxins; Metastases; Tumor cell lines; Cytotoxicity; Receptor density; Ovarian carcinoma; Antitumor activity; Pseudomonas ER - TY - JOUR T1 - Large-scale Expression and Purification of a G-protein-coupled Receptor for Structure Determination - An Overview AN - 19773601; 6873211 AB - Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands. JF - Journal of Structural and Functional Genomics AU - Grisshammer, Reinhard AU - White, Jim F AU - Trinh, Loc B AU - Shiloach, Joseph AD - National Institutes of Health, Bethesda, Maryland, 20892, USA, rkgriss@helix.nih.gov Y1 - 2005/09// PY - 2005 DA - Sep 2005 SP - 159 EP - 163 PB - Springer-Verlag (Heidelberg), Tiergartenstrasse 17 Heidelberg 69121 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 6 IS - 2-3 SN - 1345-711X, 1345-711X KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Crystallization KW - Neurotensin KW - double prime G protein-coupled receptors KW - Structure-function relationships KW - Reviews KW - Escherichia coli KW - N.M.R. KW - J 02310:Genetics & Taxonomy KW - W 30945:Fermentation & Cell Culture KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19773601?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Structural+and+Functional+Genomics&rft.atitle=Large-scale+Expression+and+Purification+of+a+G-protein-coupled+Receptor+for+Structure+Determination+-+An+Overview&rft.au=Grisshammer%2C+Reinhard%3BWhite%2C+Jim+F%3BTrinh%2C+Loc+B%3BShiloach%2C+Joseph&rft.aulast=Grisshammer&rft.aufirst=Reinhard&rft.date=2005-09-01&rft.volume=6&rft.issue=2-3&rft.spage=159&rft.isbn=&rft.btitle=&rft.title=Journal+of+Structural+and+Functional+Genomics&rft.issn=1345711X&rft_id=info:doi/10.1007%2Fs10969-005-1917-6 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Crystallization; Neurotensin; Structure-function relationships; double prime G protein-coupled receptors; Reviews; N.M.R.; Escherichia coli DO - http://dx.doi.org/10.1007/s10969-005-1917-6 ER - TY - JOUR T1 - Cardiac mitochondrial compromise in 1-yr-old Erythrocebus patas monkeys perinatally-exposed to nucleoside reverse transcriptase inhibitors AN - 19537510; 8380879 AB - Hearts from 1-yr-old Erythrocebus patas monkeys were examined after in utero and 6-wk-postbirth exposure to antiretroviral nucleoside reverse transcriptase inhibitors (NRTIs). Protocols were modeled on those given to human immunodeficiency virus (HIV)-1-infected pregnant women. NRTIs were administered daily to the dams for the last 20% or 50% of gestation, and to the infants for 6 wk after birth. Exposures included: no drug (n=4); Zidovudine, 3'-azido-3'-deoxythymidine (AZT; n=4); AZT/Lamivudine, (-)- beta -L-2', 3'-Dideoxy-3'-thiacytidine (Epivir, 3TC) (n=4); AZT/Didanosine (Videx, ddI) (n=4); and Stavudine (Zerit, d4T)/3TC (n=4). Echocardiograms and clinical chemistry showed no drug-related changes, but the d4T/3TC-exposed fetuses at 6 and 12 mo had increased white cell counts (p<0.05). At 1 yr of age, oxidative phosphorylation (OXPHOS) enzyme activities were similar in heart mitochondria from all groups. Mitochondrial pathology, that included clones of damaged mitochondria (p<0.05), was found in hearts of all 1-yr drug-exposed infants. Levels of mtDNA were elevated (p<0.05) in hearts of all NRTI-exposed monkeys in the following order: control < d4T/3TC20% per 10 years in the 70s and beyond. The rate of decline for each decade was larger in men than in women from the 40s onward. Similar longitudinal rates of decline prevailed when peak {Vdot}O sub(2) was indexed per kilogram of body weight or per kilogram of fat-free mass and in all quartiles of self-reported leisure-time physical activity. When the components of peak {Vdot}O sub(2) were examined, the rate of longitudinal decline of the oxygen pulse (ie, the O sub(2) utilization per heart beat) mirrored that of peak {Vdot}O sub(2), whereas the longitudinal rate of heart rate decline averaged only 4% to 6% per 10 years, and accelerated only minimally with age. CONCLUSIONS: The longitudinal rate of decline in peak {Vdot}O sub(2) in healthy adults is not constant across the age span in healthy persons, as assumed by cross-sectional studies, but accelerates markedly with each successive age decade, especially in men, regardless of physical activity habits. The accelerated rate of decline of peak aerobic capacity has substantial implications with regard to functional independence and quality of life, not only in healthy older persons, but particularly when disease-related deficits are superimposed. JF - Circulation AU - Fleg, Jerome L AU - Morrell, Christopher H AU - Bos, Angelo G AU - Brant, Larry J AU - Talbot, Laura A AU - Wright, Jeanette G AU - Lakatta, Edward G AD - Laboratory of Cardiovascular Science (J.L.F., C.H.M., J.G.W., E.G.L.) and Laboratory of Clinical Investigation (A.G.B.) and Research Resources Branch (L.J.B.), Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Md Y1 - 2005/08/02/ PY - 2005 DA - 2005 Aug 02 SP - 674 EP - 682 PB - Lippincott Williams & Wilkins, Inc, 530 Walnut Street Philadelphia PA 19106-3621 USA, [URL:http://www.lww.com/] VL - 112 IS - 5 SN - 0009-7322, 0009-7322 KW - Physical Education Index KW - Oxygen consumption KW - Longitudinal studies KW - Measurement KW - Men KW - Women KW - Heart rate KW - Gerontology KW - Health KW - Adults KW - Exercise KW - Lifestyle KW - Strength KW - Weight KW - Gender KW - Activities KW - Aerobic capacity KW - Treadmill ergometry KW - Heart diseases KW - Sex KW - Circulatory system KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17400337?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Circulation&rft.atitle=Accelerated+Longitudinal+Decline+of+Aerobic+Capacity+in+Healthy+Older+Adults&rft.au=Fleg%2C+Jerome+L%3BMorrell%2C+Christopher+H%3BBos%2C+Angelo+G%3BBrant%2C+Larry+J%3BTalbot%2C+Laura+A%3BWright%2C+Jeanette+G%3BLakatta%2C+Edward+G&rft.aulast=Fleg&rft.aufirst=Jerome&rft.date=2005-08-02&rft.volume=112&rft.issue=5&rft.spage=674&rft.isbn=&rft.btitle=&rft.title=Circulation&rft.issn=00097322&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2006-12-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Longitudinal studies; Oxygen consumption; Measurement; Men; Heart rate; Women; Gerontology; Health; Exercise; Adults; Lifestyle; Strength; Weight; Gender; Aerobic capacity; Activities; Treadmill ergometry; Circulatory system; Sex; Heart diseases ER - TY - JOUR T1 - Parkinson's disease: a broken nosology. AN - 85390835; pmid-16092073 AB - Parkinson's disease (PD) is a clinical diagnosis. We argue here that if we are to make progress in understanding its underlying pathogenesis, there is a need to have a pathological definition of disease that includes the presence of Lewy bodies and nigral loss in the ventrolateral tier of the pars compacta of the substantia nigra. Using such a definition, there is only one certain and known cause: mutations in the alpha-synuclein gene. However, the phenotype of this one known cause is broader than PD and encompasses Lewy body dementia.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Hardy, John AU - Lees, Andrew J AD - Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA. hardyj@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - S2 EP - S4 VL - 20 Suppl 12 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Humans KW - Lewy Bodies: pathology KW - Nerve Tissue Proteins: genetics KW - Nuclear Proteins KW - Parkinson Disease: genetics KW - *Parkinson Disease: pathology KW - Parkinson Disease: physiopathology KW - Repressor Proteins KW - Substantia Nigra: pathology KW - Ubiquitin-Protein Ligases: genetics KW - alpha-Synuclein: genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85390835?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Parkinson%27s+disease%3A+a+broken+nosology.&rft.au=Hardy%2C+John%3BLees%2C+Andrew+J&rft.aulast=Hardy&rft.aufirst=John&rft.date=2005-08-01&rft.volume=20+Suppl+12&rft.issue=&rft.spage=S2&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Effects of serotonin 5-HT1A agonist in advanced Parkinson's disease. AN - 85390177; pmid-15791634 AB - Intermittent stimulation of striatal dopaminergic receptors seems to contribute to motor dysfunction in advanced Parkinson's disease (PD). With severe dopaminergic denervation, exogenous levodopa is largely decarboxylated to dopamine in serotonergic terminals. If 5-HT1A autoreceptors regulate dopamine as well as serotonin release, in parkinsonian patients inhibition of striatal serotonergic neuron firing might help maintain more physiological intrasynaptic dopamine concentrations and thus ameliorate motor fluctuations and dyskinesias. To evaluate this hypothesis, effects of a selective 5-HT1A agonist, sarizotan, given orally at 2 and 5 mg twice daily to 18 relatively advanced parkinsonian patients, were compared with baseline placebo function during a 3-week, double-blind, placebo-controlled, proof-of-concept study. Sarizotan alone or with intravenous levodopa had no effect on parkinsonian severity. But at safe and tolerable doses, sarizotan coadministration reduced levodopa-induced dyskinesias and prolonged its antiparkinsonian response (P < or = 0.05). Under the conditions of this study, our findings suggest that 5-HT1A receptor stimulation in levodopa-treated parkinsonian patients can modulate striatal dopaminergic function and that 5-HT1A agonists may be useful as levodopa adjuvants in the treatment of PD.Copyright 2005 Movement Disorder Society JF - Movement disorders : official journal of the Movement Disorder Society AU - Bara-Jimenez, William AU - Bibbiani, Francesco AU - Morris, Michael J AU - Dimitrova, Tzvetelina AU - Sherzai, Abdullah AU - Mouradian, Maral M AU - Chase, Thomas N AD - Experimental Therapeutics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA. baraw@ninds.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 932 EP - 936 VL - 20 IS - 8 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Aged KW - *Antiparkinson Agents: therapeutic use KW - Dose-Response Relationship, Drug KW - Double-Blind Method KW - Drug Evaluation: methods KW - Female KW - Humans KW - Male KW - Middle Aged KW - Organic Chemicals KW - *Parkinson Disease: drug therapy KW - *Serotonin 5-HT1 Receptor Agonists KW - Severity of Illness Index KW - Treatment Outcome UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85390177?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Effects+of+serotonin+5-HT1A+agonist+in+advanced+Parkinson%27s+disease.&rft.au=Bara-Jimenez%2C+William%3BBibbiani%2C+Francesco%3BMorris%2C+Michael+J%3BDimitrova%2C+Tzvetelina%3BSherzai%2C+Abdullah%3BMouradian%2C+Maral+M%3BChase%2C+Thomas+N&rft.aulast=Bara-Jimenez&rft.aufirst=William&rft.date=2005-08-01&rft.volume=20&rft.issue=8&rft.spage=932&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Identification of OATP1B3 as a high-affinity hepatocellular transporter of paclitaxel. AN - 70166901; 16210916 AB - Interindividual variability in paclitaxel and docetaxel pharmacokinetics, toxicity and response is extensive, and largely unexplained. We hypothesized that this is due to affinity of taxanes for an uptake transporter that indirectly regulates elimination pathways. Here, we studied accumulation of [3H]docetaxel and [3H]paclitaxel in Xenopus laevis oocytes injected with cRNA of the liver-specific organic anion transporting polypeptide (OATP) family members OATP1B1 (OATP2) or OATP1B3 (OATP8). Taxane transport by OATP1B1 expressing oocytes was not significantly different from that by water-injected controls, whereas uptake by OATP1B3 was 2.2-fold higher for docetaxel (p = 0.0007) and 3.3-fold higher for paclitaxel (p < 0.0001). OATP1B3-mediated paclitaxel transport was saturable (Michaelis-Menten constant, 6.79 microM), time-dependent, and highly sensitive to chemical inhibition. Paclitaxel uptake was not inhibited by ketoconazole or tariquidar. However, uptake was inhibited by the formulation excipient Cremophor (74.4% inhibition, p < 0.0001), cyclosporin A (25.2%, p = 0.005), glycyrrhizic acid (24.6%, p = 0.012), and hyperforin (28.4%, p = 0.003). Consistent with this finding, Cremophor was found to significantly affect the hepatic uptake of paclitaxel in mice. These data suggest that OATP1B3 is a key regulator of hepatic uptake, and may therefore play a role in the variable response to treatment with taxanes. JF - Cancer biology & therapy AU - Smith, Nicola F AU - Acharya, Milin R AU - Desai, Neil AU - Figg, William D AU - Sparreboom, Alex AD - Molecular Pharmacology Section, Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 815 EP - 818 VL - 4 IS - 8 SN - 1538-4047, 1538-4047 KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Organic Anion Transporters KW - Organic Anion Transporters, Sodium-Independent KW - Quinolines KW - SLCO1B1 protein, human KW - SLCO1B3 protein, human KW - Solute Carrier Organic Anion Transporter Family Member 1b1 KW - tariquidar KW - J58862DTVD KW - Paclitaxel KW - P88XT4IS4D KW - Ketoconazole KW - R9400W927I KW - Index Medicus KW - Animals KW - Hepatocytes -- drug effects KW - Humans KW - Mice KW - Xenopus laevis KW - Mice, Inbred Strains KW - Organic Anion Transporters -- genetics KW - Quinolines -- pharmacology KW - Oocytes -- metabolism KW - Organic Anion Transporters -- physiology KW - Ketoconazole -- pharmacology KW - Female KW - Hepatocytes -- metabolism KW - Organic Anion Transporters, Sodium-Independent -- genetics KW - Paclitaxel -- metabolism KW - Liver -- cytology KW - Organic Anion Transporters, Sodium-Independent -- antagonists & inhibitors KW - Liver -- drug effects KW - Organic Anion Transporters, Sodium-Independent -- physiology KW - Liver -- metabolism KW - Antineoplastic Agents, Phytogenic -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70166901?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+biology+%26+therapy&rft.atitle=Identification+of+OATP1B3+as+a+high-affinity+hepatocellular+transporter+of+paclitaxel.&rft.au=Smith%2C+Nicola+F%3BAcharya%2C+Milin+R%3BDesai%2C+Neil%3BFigg%2C+William+D%3BSparreboom%2C+Alex&rft.aulast=Smith&rft.aufirst=Nicola&rft.date=2005-08-01&rft.volume=4&rft.issue=8&rft.spage=815&rft.isbn=&rft.btitle=&rft.title=Cancer+biology+%26+therapy&rft.issn=15384047&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-07-05 N1 - Date created - 2006-05-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Cancer Biol Ther. 2005 Sep;4(9):1030-2 [16177563] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Elevated plasma concentrations of protease inhibitors and nonnucleoside reverse transcriptase inhibitors in patients coinfected with human immunodeficiency virus and hepatitis B or C: case series and literature review. AN - 68661687; 16207097 AB - To evaluate antiretroviral pharmacokinetics in patients who are coinfected with human immunodeficiency virus (HIV) and hepatitis B and/or C virus. Specifically, we sought to determine whether coinfection results in higher than expected concentrations of protease inhibitors and nonnucleoside reverse transcriptase inhibitors (NNRTIs). Case series. Human immunodeficiency virus clinic. Twenty-six patients infected with HIV and hepatitis B and/or C virus. Patients' plasma trough concentrations (Cmeasured) of protease inhibitors and NNRTIs were compared with population average trough concentrations reported in the literature (Cpredicted). Trough concentrations were obtained irrespective of the patients' liver function. A concentration ratio of Cmeasured: Cpredicted was determined for each patient. The mean concentration ratio of the 26 patients was 1.43 (95% confidence interval 1.08-1.78). For the six patients taking nelfinavir, the ratio of nelfinavir's active metabolite (M8): parent drug was calculated. The median M8:nelfinavir ratio for these six patients was 69% lower than what has been reported in a general HIV population. These preliminary findings suggest that trough concentrations of protease inhibitors and NNRTIs may be elevated in patients with HIV infection who are coinfected with hepatitis B and/or C, compared with a general population of patients with HIV infection. Until further investigation defines the relationship between coinfection, metabolic dysfunction, and increased antiretroviral exposure, therapeutic drug monitoring may be helpful to identify coinfected patients at risk for antiretroviral toxicity secondary to elevated plasma drug concentrations. JF - Pharmacotherapy AU - Robertson, Sarah M AU - Scarsi, Kimberly K AU - Postelnick, Michael J AU - Lynch, Patrick AD - Clinical Pharmacokinetics Research Laboratory, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. robertsonsa@cc.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1068 EP - 1072 VL - 25 IS - 8 SN - 0277-0008, 0277-0008 KW - HIV Protease Inhibitors KW - 0 KW - Reverse Transcriptase Inhibitors KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - Index Medicus KW - Liver Cirrhosis -- pathology KW - Half-Life KW - Humans KW - HIV Reverse Transcriptase -- antagonists & inhibitors KW - Retrospective Studies KW - Liver Function Tests KW - Chromatography, High Pressure Liquid KW - Hepatitis B -- complications KW - Reverse Transcriptase Inhibitors -- pharmacokinetics KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - Hepatitis B -- metabolism KW - HIV Protease Inhibitors -- blood KW - HIV Infections -- metabolism KW - Reverse Transcriptase Inhibitors -- blood KW - HIV Protease Inhibitors -- pharmacokinetics KW - HIV Protease Inhibitors -- therapeutic use KW - Reverse Transcriptase Inhibitors -- therapeutic use KW - Hepatitis C -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68661687?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacotherapy&rft.atitle=Elevated+plasma+concentrations+of+protease+inhibitors+and+nonnucleoside+reverse+transcriptase+inhibitors+in+patients+coinfected+with+human+immunodeficiency+virus+and+hepatitis+B+or+C%3A+case+series+and+literature+review.&rft.au=Robertson%2C+Sarah+M%3BScarsi%2C+Kimberly+K%3BPostelnick%2C+Michael+J%3BLynch%2C+Patrick&rft.aulast=Robertson&rft.aufirst=Sarah&rft.date=2005-08-01&rft.volume=25&rft.issue=8&rft.spage=1068&rft.isbn=&rft.btitle=&rft.title=Pharmacotherapy&rft.issn=02770008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-10-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Reversibility of experimental rabbit liver cirrhosis by portal collagenase administration. AN - 68657800; 15965490 AB - The regression of cirrhosis is associated with increased intrahepatic collagenolytic enzyme activity. We investigated whether collagenase supplementation via portal vein infusion can retard cirrhosis development and/or reverse cirrhosis. In all, 35 rabbits were initially assigned to study. However, because of high surgical mortality and infection, only 15 animals completed study. Four normal controls (group I) received olive oil subcutaneously (SC) for 12 weeks followed by normal saline portal perfusion for 12 weeks. Four (group II) received CCl(4) SC for 6 weeks followed by portal vein collagenase, 6 mg twice weekly, plus SC CCl(4) for 6 additional weeks and then killed. Four rabbits (group III) received CCl(4) SC for 12 weeks and then 6 mg of collagenase portally for 12 weeks, while three control rabbits (group IV) received CCl(4) for 12 weeks followed by saline for 12 weeks. After 12 weeks of CCl(4), liver hydroxyproline content of collagenase-treated group II (361.1+/-106.6 microg/g) was significantly reduced compared with group III+IV that had not yet received collagenase (589.0+/-162.9 microg/g; P<0.05). In the main comparison, hydroxyproline content of collagenase-treated group III (177.5+/-35.6 microg/g) was significantly decreased compared with saline-treated controls (446.3+/-150.1 microg/g; P<0.01). Further, liver histology showed complete regression of cirrhosis in the collagenase-treated animals. No toxicity of liver, kidney, lung, brain or heart was observed histologically. Anaphylaxis occurred in 2/35 original animals (one fatal). In conclusion, this study provides 'proof of principle' that collagenase portal administration can retard cirrhosis development and speed regression of established cirrhosis in the rabbit CCl(4) model. Potential application to humans is premature, but feasible, if these findings are confirmed in additional animal studies. JF - Laboratory investigation; a journal of technical methods and pathology AU - Jin, Bo AU - Alter, Harvey J AU - Zhang, Zhi-Cheng AU - Shih, J Wai-Kuo AU - Esteban, Juan M AU - Sun, Tao AU - Yang, Yun-Sheng AU - Qiu, Qi AU - Liu, Xiao-Lin AU - Yao, Lin AU - Wang, Hai-Dong AU - Cheng, Liu-Fang AD - Department of Digestive Diseases, Military Medical Graduate School, Beijing, China. bjin@cc.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 992 EP - 1002 VL - 85 IS - 8 SN - 0023-6837, 0023-6837 KW - Collagen KW - 9007-34-5 KW - Carbon Tetrachloride KW - CL2T97X0V0 KW - Collagenases KW - EC 3.4.24.- KW - Index Medicus KW - Animals KW - Infusions, Intravenous KW - Collagen -- metabolism KW - Portal Vein KW - Liver -- metabolism KW - Rabbits KW - Carbon Tetrachloride -- toxicity KW - Male KW - Liver Cirrhosis -- chemically induced KW - Liver Cirrhosis -- drug therapy KW - Liver Cirrhosis -- metabolism KW - Collagenases -- administration & dosage KW - Collagenases -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68657800?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Laboratory+investigation%3B+a+journal+of+technical+methods+and+pathology&rft.atitle=Reversibility+of+experimental+rabbit+liver+cirrhosis+by+portal+collagenase+administration.&rft.au=Jin%2C+Bo%3BAlter%2C+Harvey+J%3BZhang%2C+Zhi-Cheng%3BShih%2C+J+Wai-Kuo%3BEsteban%2C+Juan+M%3BSun%2C+Tao%3BYang%2C+Yun-Sheng%3BQiu%2C+Qi%3BLiu%2C+Xiao-Lin%3BYao%2C+Lin%3BWang%2C+Hai-Dong%3BCheng%2C+Liu-Fang&rft.aulast=Jin&rft.aufirst=Bo&rft.date=2005-08-01&rft.volume=85&rft.issue=8&rft.spage=992&rft.isbn=&rft.btitle=&rft.title=Laboratory+investigation%3B+a+journal+of+technical+methods+and+pathology&rft.issn=00236837&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-31 N1 - Date created - 2005-10-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neuropsychiatric alterations in MDMA users: preliminary findings. AN - 68611315; 16179502 AB - The use of marijuana is rampant among 3,4-methylenedioxymethamphetamine (MDMA) users. The co-occurrence of abuse of these two drugs has made it difficult to assess the specific residual effects of MDMA alone. As a first step toward identifying the effects of long-term MDMA use, we studied 8 MDMA abusers, 8 marijuana/MDMA abusers, 15 marijuana abusers (matched in marijuana use without MDMA use), and 17 control subjects. EEG, cerebral blood velocity by pulsed transcranial Doppler (TCD), and psychological measures were collected. Three-minute resting eyes-closed EEG recordings were obtained from 16 electrodes. The EEG was converted to 6 frequency bands (delta, theta, alpha-1, alpha-2, beta-1, and beta-2) using a fast Fourier transformation. Blood flow velocity was determined using a temporal window for the right and left middle cerebral arteries using TCD. Absolute log delta power in the EEG of MDMA abusers at central electrode sites was significantly higher than that of the MDMA/marijuana, marijuana abusers, and control subjects. There were also increases in alpha-2 EEG power observed only in marijuana abusers. The blood flow measure, diastolic velocity, was increased in MDMA abusers whether they used marijuana or not. Because increases in delta power and perfusion deficits are associated with some chronic disorders, our findings in these ecstasy abusers suggest that MDMA use may be associated with a drug-induced neuropathological state. More research is necessary to test these ideas. JF - Annals of the New York Academy of Sciences AU - Herning, Ronald I AU - Better, Warren AU - Tate, Kimberly AU - Cadet, Jean L AD - Molecular Neuropsychiatry Branch, National Institute on Drug Abuse, Intramural Research Program, 5500 Nathan Shock Drive, Baltimore, Maryland 21224. rherning@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 20 EP - 27 VL - 1053 SN - 0077-8923, 0077-8923 KW - Hallucinogens KW - 0 KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Index Medicus KW - Humans KW - Cerebrovascular Circulation -- drug effects KW - Electroencephalography KW - Adult KW - Ultrasonography, Doppler, Transcranial KW - Marijuana Abuse -- psychology KW - Neuropsychological Tests KW - Marijuana Abuse -- physiopathology KW - Male KW - Female KW - Psychological Tests KW - Mental Disorders -- chemically induced KW - Mental Disorders -- psychology KW - Mental Disorders -- etiology KW - Substance-Related Disorders -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68611315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Neuropsychiatric+alterations+in+MDMA+users%3A+preliminary+findings.&rft.au=Herning%2C+Ronald+I%3BBetter%2C+Warren%3BTate%2C+Kimberly%3BCadet%2C+Jean+L&rft.aulast=Herning&rft.aufirst=Ronald&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=20&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microglial NADPH oxidase mediates leucine enkephalin dopaminergic neuroprotection. AN - 68611124; 16179514 AB - Here, we report that leucine enkephalin (LE) is neuroprotective to dopaminergic (DA) neurons at femtomolar concentrations through anti-inflammatory properties. Mesencephalic neuron-glia cultures pretreated with femtomolar concentrations of LE (10(-15)-10(-13) M) protected DA neurons from lipopolysaccharide (LPS)-induced DA neurotoxicity, as determined by DA uptake assay and tyrosine hydroxylase (TH) immunocytochemistry (ICC). However, des-tyrosine leucine enkephalin (DTLE), an LE analogue that is missing the tyrosine residue required for binding to the kappa opioid receptor, was also neuroprotective (10(-15)-10(-13) M), as determined by DA uptake assay and TH ICC. Both LE and DTLE (10(-15)-10(-13) M) reduced LPS-induced superoxide production from microglia-enriched cultures. Further, both LE and DTLE (10(-14), 10(-13) M) reduced the LPS-induced tumor necrosis factor-alpha (TNFalpha) mRNA and TNFalpha protein from PHOX+/+ microglia, as determined by quantitative real-time RT-PCR and ELISA analysis in mesencephalic neuron-glia cultures, respectively. However, both peptides failed to inhibit TNFalpha expression in PHOX-/- cultures, which are unable to produce extracellular superoxide in response to LPS. Additionally, LE and DTLE (10(-14), 10(-13) M) failed to show any neuroprotection against LPS in PHOX-/- cultures. Together, these data indicate that LE and DTLE are neuroprotective at femtomolar concentrations through the inhibition of oxidative insult associated with microglial NADPH oxidase and the attenuation of the ROS-mediated amplification of TNFalpha gene expression in microglia. JF - Annals of the New York Academy of Sciences AU - Qin, Liya AU - Liu, Yuxin AU - Qian, Xun AU - Hong, Jau-Shyong AU - Block, Michelle L AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 107 EP - 120 VL - 1053 SN - 0077-8923, 0077-8923 KW - Anti-Inflammatory Agents KW - 0 KW - Indicators and Reagents KW - Lipopolysaccharides KW - Neuroprotective Agents KW - Oligopeptides KW - Tumor Necrosis Factor-alpha KW - Superoxides KW - 11062-77-4 KW - Enkephalin, Leucine KW - 58822-25-6 KW - deltakephalin KW - 85286-38-0 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Animals KW - Neurons -- drug effects KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - Pregnancy KW - Rats KW - Superoxides -- metabolism KW - Rats, Inbred F344 KW - Cells, Cultured KW - Enzyme-Linked Immunosorbent Assay KW - Lipopolysaccharides -- toxicity KW - Neurons -- enzymology KW - Oligopeptides -- pharmacology KW - Tumor Necrosis Factor-alpha -- metabolism KW - Immunohistochemistry KW - Female KW - Microglia -- enzymology KW - Dopamine -- physiology KW - NADPH Oxidase -- physiology KW - NADPH Oxidase -- genetics KW - Microglia -- drug effects KW - Enkephalin, Leucine -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68611124?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Microglial+NADPH+oxidase+mediates+leucine+enkephalin+dopaminergic+neuroprotection.&rft.au=Qin%2C+Liya%3BLiu%2C+Yuxin%3BQian%2C+Xun%3BHong%2C+Jau-Shyong%3BBlock%2C+Michelle+L&rft.aulast=Qin&rft.aufirst=Liya&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The antiapoptotic actions of mood stabilizers: molecular mechanisms and therapeutic potentials. AN - 68610580; 16179524 AB - Two primary drugs used to treat bipolar mood disorder are lithium and valproate. Emerging evidence supports the notion that both mood stabilizers have neuroprotective effects. In primary cultures of rat cerebellar granule cells and cortical neurons, lithium and valproate robustly and potently protect against glutamate-induced, N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. The neuroprotective mechanisms involve inactivation of NMDA receptors through inhibition of NR2B tyrosine phosphorylation, activation of cell survival factors such as the PI 3-kinase/Akt signaling pathway, and induction of neurotrophic/neuroprotective proteins, including brain-derived neurotrophic factor, heat-shock protein (HSP), and Bcl-2. Both drugs are also effective against other forms of insults such as ER stress in neurally related cell types. The molecular targets likely involve glycogen synthase kinase-3 (GSK-3) and histone deacetylase (HDAC) for lithium and valproate, respectively. In a rat cerebral artery occlusion model of stroke, postinsult treatment with lithium or valproate reduces ischemia-induced brain infarction, caspase-3 activation, and neurological deficits, and these neuroprotective effects are associated with HSP70 upregulation and, in the case of valproate, HDAC inhibition. In a rat excitotoxic model of Huntington's disease in which an excitotoxin is infused into the striatum to activate NMDA receptors, short-term lithium pretreatment is sufficient to protect against DNA damage, caspase activation, and apoptosis of striatal neurons, and this neuroprotection is concurrent with Bcl-2 induction. Moreover, lithium treatment increases cell proliferation near the site of striatal injury, and some newborn cells have phenotypes of neurons and astroglia. Thus, lithium and valproate are potential drugs for treating some forms of neurodegenerative diseases. JF - Annals of the New York Academy of Sciences AU - Chuang, De-Maw AD - Molecular Neurobiology Section, Biological Psychiatry Branch, Mood and Anxiety Disorders Program, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1363, USA. chuang@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 195 EP - 204 VL - 1053 SN - 0077-8923, 0077-8923 KW - Antimanic Agents KW - 0 KW - Excitatory Amino Acid Agonists KW - Valproic Acid KW - 614OI1Z5WI KW - N-Methylaspartate KW - 6384-92-5 KW - Lithium KW - 9FN79X2M3F KW - Index Medicus KW - Valproic Acid -- pharmacology KW - Rats KW - Stroke -- drug therapy KW - Animals KW - Stroke -- pathology KW - Humans KW - N-Methylaspartate -- toxicity KW - N-Methylaspartate -- antagonists & inhibitors KW - Lithium -- therapeutic use KW - Valproic Acid -- therapeutic use KW - Excitatory Amino Acid Agonists -- toxicity KW - Lithium -- pharmacology KW - Antimanic Agents -- pharmacology KW - Affect -- drug effects KW - Apoptosis -- drug effects KW - Antimanic Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68610580?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=The+antiapoptotic+actions+of+mood+stabilizers%3A+molecular+mechanisms+and+therapeutic+potentials.&rft.au=Chuang%2C+De-Maw&rft.aulast=Chuang&rft.aufirst=De-Maw&rft.date=2005-08-01&rft.volume=1053&rft.issue=&rft.spage=195&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-06 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - D2 but not D1 dopamine receptor stimulation augments brain signaling involving arachidonic acid in unanesthetized rats. AN - 68586535; 16163535 AB - Signal transduction involving the activation of phospholipase A2 (PLA2) to release arachidonic acid (AA) from membrane phospholipids, when coupled to dopamine D1- and D2-type receptors, can be imaged in rats having a chronic unilateral lesion of the substantia nigra. It is not known, however, if the signaling responses occur in the absence of a lesion. To determine this, we used our in vivo fatty acid method to measure signaling in response to D1 and D2 receptor agonists given acutely to unanesthetized rats. [1-(14)C]AA was injected intravenously in unanesthetized rats, and incorporation coefficients k* for AA (brain radioactivity/integrated plasma radioactivity) were measured using quantitative autoradiography in 61 brain regions. The animals were administered i.v. the D2 receptor agonist, quinpirole (1 mg kg(-1), i.v.), the D1 receptor agonist SKF-38393 (5 mg kg(-1), i.v.), or vehicle/saline. Quinpirole increased k* significantly in multiple brain regions rich in D2-type receptors, whereas SKF-38393 did not change k* significantly in any of the 61 regions examined. In the intact rat brain, D2 but not D1 receptors are coupled to the activation of PLA2 and the release of AA. JF - Psychopharmacology AU - Bhattacharjee, Abesh Kumar AU - Chang, Lisa AU - Lee, Ho-Joo AU - Bazinet, Richard P AU - Seemann, Ruth AU - Rapoport, Stanley I AD - Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bldg. 9, Room 1S128, Bethesda, MD 20892, USA. abeshb@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 735 EP - 742 VL - 180 IS - 4 SN - 0033-3158, 0033-3158 KW - Carbon Isotopes KW - 0 KW - Dopamine Agonists KW - Receptors, Dopamine D1 KW - Receptors, Dopamine D2 KW - Quinpirole KW - 20OP60125T KW - Arachidonic Acid KW - 27YG812J1I KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine KW - 67287-49-4 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Quinpirole -- pharmacology KW - Drug Interactions KW - Dopamine Agonists -- pharmacology KW - Carbon Isotopes -- pharmacokinetics KW - Autoradiography -- methods KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine -- pharmacology KW - Wakefulness KW - Male KW - Drug Administration Routes KW - Signal Transduction -- physiology KW - Brain -- drug effects KW - Arachidonic Acid -- pharmacokinetics KW - Signal Transduction -- drug effects KW - Brain -- physiology KW - Receptors, Dopamine D1 -- metabolism KW - Arachidonic Acid -- metabolism KW - Receptors, Dopamine D2 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68586535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=D2+but+not+D1+dopamine+receptor+stimulation+augments+brain+signaling+involving+arachidonic+acid+in+unanesthetized+rats.&rft.au=Bhattacharjee%2C+Abesh+Kumar%3BChang%2C+Lisa%3BLee%2C+Ho-Joo%3BBazinet%2C+Richard+P%3BSeemann%2C+Ruth%3BRapoport%2C+Stanley+I&rft.aulast=Bhattacharjee&rft.aufirst=Abesh&rft.date=2005-08-01&rft.volume=180&rft.issue=4&rft.spage=735&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-17 N1 - Date created - 2005-09-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Therapy insight: AIDS-related malignancies--the influence of antiviral therapy on pathogenesis and management. AN - 68535738; 16130937 AB - Patients with HIV infection are at an increased risk of a number of malignancies, including Kaposi's sarcoma (KS) and certain B-cell lymphomas. Most of these tumors are caused by oncogenic DNA viruses, including KS-associated herpesvirus and Epstein-Barr virus. HIV contributes to the development of these tumors through several mechanisms, including immunodeficiency, immunodysregulation, and the effects of HIV proteins such as Tat. The development of highly active antiretroviral therapy (HAART) has reduced the incidence of many HIV-associated tumors and has generally improved their responsiveness to therapy. However, the number of people living with AIDS is increasing, and it is possible that the number of AIDS-associated malignancies will rise and the pattern of tumors will change as more people live longer with HIV infection. The goal of KS therapy is long-term tumor control with minimal toxicity. HAART is an important component of this therapy, and some patients do not require other KS-specific therapies. By contrast, the goal of AIDS-related lymphoma therapy in most cases is the attainment of a complete response with curative intent, and the benefits of administering HAART during therapy must be weighed against possible disadvantages. The past decade has seen substantial improvements in the treatment of AIDS-related lymphoma, which is attributed partially to a shift in tumor type and more effective regimens. There is currently an interest in developing new therapies for HIV-associated malignancies, based on viral, vascular or other pathogenesis-based targets. JF - Nature clinical practice. Oncology AU - Yarchoan, Robert AU - Tosato, Giovanna AU - Little, Richard F AD - HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1868, USA. yarchoan@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 406 EP - 15; quiz 423 VL - 2 IS - 8 SN - 1743-4254, 1743-4254 KW - Antineoplastic Agents KW - 0 KW - Index Medicus KW - Sarcoma, Kaposi -- drug therapy KW - Sarcoma, Kaposi -- physiopathology KW - Humans KW - Lymphoma, AIDS-Related -- physiopathology KW - Lymphoma, AIDS-Related -- drug therapy KW - Sarcoma, Kaposi -- virology KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Neoplasms -- drug therapy KW - Neoplasms -- virology KW - HIV Infections -- complications KW - Antineoplastic Agents -- administration & dosage KW - Neoplasms -- physiopathology KW - Antiretroviral Therapy, Highly Active KW - Antineoplastic Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68535738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+clinical+practice.+Oncology&rft.atitle=Therapy+insight%3A+AIDS-related+malignancies--the+influence+of+antiviral+therapy+on+pathogenesis+and+management.&rft.au=Yarchoan%2C+Robert%3BTosato%2C+Giovanna%3BLittle%2C+Richard+F&rft.aulast=Yarchoan&rft.aufirst=Robert&rft.date=2005-08-01&rft.volume=2&rft.issue=8&rft.spage=406&rft.isbn=&rft.btitle=&rft.title=Nature+clinical+practice.+Oncology&rft.issn=17434254&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-08-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Agricultural antibiotics and human health. AN - 68517902; 15984910 JF - PLoS medicine AU - Smith, David L AU - Dushoff, Jonathan AU - Morris, J Glenn AD - Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA. smitdave@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1 VL - 2 IS - 8 KW - Anti-Bacterial Agents KW - 0 KW - Food Additives KW - Glycopeptides KW - Vancomycin KW - 6Q205EH1VU KW - avoparcin KW - WJ13O9MNTI KW - Index Medicus KW - Animals KW - Vancomycin -- adverse effects KW - Animal Feed KW - Humans KW - Enterococcus -- drug effects KW - Glycopeptides -- adverse effects KW - Animals, Domestic -- growth & development KW - Vancomycin Resistance KW - Animals, Domestic -- microbiology KW - Community-Acquired Infections -- transmission KW - Agriculture -- methods KW - Public Health KW - Food Additives -- adverse effects KW - Anti-Bacterial Agents -- adverse effects KW - Community-Acquired Infections -- microbiology KW - Zoonoses -- microbiology KW - Disease Transmission, Infectious KW - Zoonoses -- transmission UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68517902?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=PLoS+medicine&rft.atitle=Agricultural+antibiotics+and+human+health.&rft.au=Smith%2C+David+L%3BDushoff%2C+Jonathan%3BMorris%2C+J+Glenn&rft.aulast=Smith&rft.aufirst=David&rft.date=2005-08-01&rft.volume=2&rft.issue=8&rft.spage=e232&rft.isbn=&rft.btitle=&rft.title=PLoS+medicine&rft.issn=1549-1676&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-08-18 N1 - Date created - 2005-08-25 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Acta Vet Scand Suppl. 1999;92:51-7 [10783717] Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):3153-8 [15677330] J Antimicrob Chemother. 2000 May;45(5):677-80 [10797092] J Infect Dis. 2000 Sep;182(3):816-23 [10950776] Clin Microbiol Rev. 2000 Oct;13(4):686-707 [11023964] Eur J Clin Microbiol Infect Dis. 2000 Nov;19(11):816-22 [11152305] Appl Environ Microbiol. 2001 Apr;67(4):1494-502 [11282596] Emerg Infect Dis. 2001 Mar-Apr;7(2):183-7 [11294702] Nature. 2001 Jan 4;409(6816):37-8 [11343104] Antimicrob Agents Chemother. 2001 Jul;45(7):2054-9 [11408222] Vet Res. 2001 May-Aug;32(3-4):311-21 [11432422] Philos Trans R Soc Lond B Biol Sci. 2001 Jul 29;356(1411):983-9 [11516376] N Engl J Med. 2001 Oct 18;345(16):1161-6 [11642232] Clin Infect Dis. 2001 Nov 15;33(10):1739-46 [11595995] Clin Microbiol Infect. 2001;7 Suppl 4:16-33 [11688531] Lancet Infect Dis. 2001 Dec;1(5):314-25 [11871804] Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6434-9 [11972035] Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5752-4 [11983874] Int J Food Microbiol. 2002 Jun 5;76(1-2):143-50 [12038571] Antimicrob Agents Chemother. 2002 Sep;46(9):2779-83 [12183228] Lancet Infect Dis. 2003 Apr;3(4):241-9 [12679267] Int J Food Microbiol. 2003 Aug 1;84(3):273-84 [12810291] Int J Food Microbiol. 2003 Aug 25;85(3):213-26 [12878380] APMIS. 2003 Jun;111(6):669-72 [12969023] Emerg Infect Dis. 2003 Sep;9(9):1108-15 [14519248] Environ Health Perspect. 2003 Oct;111(13):1590-4 [14527837] Antimicrob Agents Chemother. 2003 Dec;47(12):3825-30 [14638490] J Antimicrob Chemother. 2004 Jan;53(1):28-52 [14657094] Environ Microbiol. 2004 Jan;6(1):55-9 [14686941] Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3709-14 [14985511] Clin Infect Dis. 2004 Apr 15;38(8):1108-15 [15095215] J Antimicrob Chemother. 2004 Jul;54(1):274-5; author reply 276-8 [15140862] Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10223-8 [15220470] J Antimicrob Chemother. 2004 Aug;54(2):311-20 [15215223] Int J Antimicrob Agents. 2004 Sep;24(3):205-12 [15325422] Ann Intern Med. 1995 Aug 15;123(4):250-9 [7611590] J Clin Microbiol. 1995 Nov;33(11):2842-6 [8576330] J Infect Dis. 1996 May;173(5):1129-36 [8627064] Lancet. 1997 Apr 26;349(9060):1258 [9130981] Lancet. 1997 Jul 12;350(9071):146-7 [9228991] J Antimicrob Chemother. 1997 Sep;40(3):454-6 [9338505] J Clin Microbiol. 1997 Dec;35(12):3026-31 [9399488] J Infect Dis. 1998 Feb;177(2):378-82 [9466524] Antimicrob Agents Chemother. 1998 May;42(5):1303-4 [9593175] J Clin Microbiol. 1998 Jul;36(7):1927-32 [9650938] Infect Control Hosp Epidemiol. 1998 Aug;19(8):539-45 [9758052] Emerg Infect Dis. 1999 May-Jun;5(3):329-35 [10341169] Antimicrob Agents Chemother. 1999 Sep;43(9):2215-21 [10471567] Nature. 1999 Sep 16;401(6750):233-4 [10499578] Comment In: PLoS Med. 2005 Nov;2(11):e383 [16288558] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Herbs and cytotoxic drugs: recognizing and communicating potentially relevant interactions. AN - 68508918; 16117219 JF - Clinical journal of oncology nursing AU - Lee, Colleen O AD - Office of Complementary and Alternative Medicine, National Cancer Institute, Bethesda, MD, USA. leeco@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 481 EP - 487 VL - 9 IS - 4 SN - 1092-1095, 1092-1095 KW - Antineoplastic Agents KW - 0 KW - Nursing KW - Nursing Assessment KW - Humans KW - Neoplasms -- drug therapy KW - Antineoplastic Agents -- administration & dosage KW - Herb-Drug Interactions KW - Antineoplastic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68508918?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+journal+of+oncology+nursing&rft.atitle=Herbs+and+cytotoxic+drugs%3A+recognizing+and+communicating+potentially+relevant+interactions.&rft.au=Lee%2C+Colleen+O&rft.aulast=Lee&rft.aufirst=Colleen&rft.date=2005-08-01&rft.volume=9&rft.issue=4&rft.spage=481&rft.isbn=&rft.btitle=&rft.title=Clinical+journal+of+oncology+nursing&rft.issn=10921095&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-08-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dopamine-dependent and dopamine-independent actions of cocaine as revealed by brain thermorecording in freely moving rats. AN - 68504823; 16115216 AB - Brain temperature fluctuates biphasically in response to repeated, intravenous (i.v.) cocaine injections, perhaps reflecting cocaine's inhibiting effect on both dopamine (DA) transporters and Na+ channels. By using a DA receptor blockade, one could separate these actions and determine the role of DA-dependent and DA-independent mechanisms in mediating this temperature fluctuation. Rats were chronically implanted with thermocouple probes in the brain, a non-locomotor head muscle and subcutaneously. Temperature fluctuations associated with ten repeated i.v. cocaine injections (1 mg/kg with 8-min inter-injection intervals) were examined after a combined, systemic administration of selective D1-like and D2-like receptor blockers (SCH-23390 and eticlopride) at doses that effectively inhibit DA transmission. In contrast to the initial temperature increases and subsequent biphasic fluctuations (decreases followed by increases) seen with repeated cocaine injections in saline-treated control, brain and muscle temperatures during DA receptor blockade decreased with each repeated cocaine injection. DA receptor blockade had no effects on skin temperature, which tonically decreased and biphasically fluctuated (decreases followed by increases) during repeated cocaine injections in both conditions. DA receptor blockade by itself slightly increased brain and muscle temperatures, with no evident effect on skin temperature. DA antagonists also strongly decreased spontaneous movement activity and completely blocked the locomotor activation normally induced by repeated cocaine injections. Although our data confirm that cocaine's inhibitory action on presynaptic DA uptake is essential for its ability to induce metabolic and behavioral activation, they also suggest that the physiological effects of this drug cannot be explained through this system alone. The continued hypothermic effect of cocaine points to its action on other central systems (particularly blockade of Na+ channels) that may be important for the development of cocaine abuse and adverse effects of this drug. JF - The European journal of neuroscience AU - Kiyatkin, Eugene A AU - Brown, P Leon AD - Cellular Neurobiology Branch, National Institute on Drug Abuse-Intramural Research Program, National Institutes of Health, DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. ekiyatki@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 930 EP - 938 VL - 22 IS - 4 SN - 0953-816X, 0953-816X KW - Benzazepines KW - 0 KW - Dopamine Antagonists KW - Dopamine Uptake Inhibitors KW - Salicylamides KW - Cocaine KW - I5Y540LHVR KW - eticlopride KW - J8M468HBH4 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats KW - Animals KW - Drug Interactions KW - Benzazepines -- pharmacology KW - Rats, Long-Evans KW - Dopamine Antagonists -- pharmacology KW - Salicylamides -- pharmacology KW - Motor Activity -- drug effects KW - Time Factors KW - Dopamine -- pharmacology KW - Body Temperature -- drug effects KW - Nucleus Accumbens -- drug effects KW - Nucleus Accumbens -- physiology KW - Wakefulness -- physiology KW - Body Temperature -- physiology KW - Cocaine -- pharmacology KW - Dopamine Uptake Inhibitors -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68504823?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+European+journal+of+neuroscience&rft.atitle=Dopamine-dependent+and+dopamine-independent+actions+of+cocaine+as+revealed+by+brain+thermorecording+in+freely+moving+rats.&rft.au=Kiyatkin%2C+Eugene+A%3BBrown%2C+P+Leon&rft.aulast=Kiyatkin&rft.aufirst=Eugene&rft.date=2005-08-01&rft.volume=22&rft.issue=4&rft.spage=930&rft.isbn=&rft.btitle=&rft.title=The+European+journal+of+neuroscience&rft.issn=0953816X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-18 N1 - Date created - 2005-08-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inflammation and cancer: when NF-kappaB amalgamates the perilous partnership. AN - 68479663; 16101381 AB - Chronic inflammation has long been suggested to constitute a risk factor for a variety of epithelial cancers such as malignancies of prostate, cervix, esophagus, stomach, liver, colon, pancreas, and bladder. An inflammatory response is typically accompanied by generation of free radicals, stimulation of cytokines, chemokines, growth and angiogenic factors. Free radicals, capable of both directly damaging DNA and affecting the DNA repair machinery, enhance genetic instability of affected cells, thus contributing to the first stage of neoplastic transformation also known as "initiation". Cytokines and growth factors can further promote tumor growth by stimulating cell proliferation, adhesion, vascularization, and metastatic potential of later stage tumors. Nuclear factor kappa B (NF-kappaB) is a family of ubiquitously expressed transcription factors that are widely believed to trigger both the onset and the resolution of inflammation. NF-kappaB also governs the expression of genes encoding proteins essential in control of stress response, maintenance of intercellular communications, and regulation of cellular proliferation and apoptosis. Recent data have expanded the concept of inflammation as a critical component in carcinogenesis suggesting new anti-inflammatory therapies for a complementary approach in treating a variety of tumor types. These observations highlighted the NF-kappaB pathway as an attractive avenue for drug discovery and development. The present review will outline recent advances in our understanding of NF-kappaB function in the inflammatory processes and its input in tumor initiation/promotion, as well as summarize the development of animal and cell culture models for validating drug candidates with NF-kappaB-modulating activities, and applications of the latter in cancer therapy. JF - Current cancer drug targets AU - Dobrovolskaia, Marina A AU - Kozlov, Serguei V AD - National Cancer Institute at Frederick, Boyles St., P.O. Box B, Frederick, MD 21702, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 325 EP - 344 VL - 5 IS - 5 SN - 1568-0096, 1568-0096 KW - Antineoplastic Agents KW - 0 KW - Cytokines KW - Free Radicals KW - Membrane Glycoproteins KW - NF-kappa B KW - Receptors, Cell Surface KW - Receptors, Tumor Necrosis Factor, Type II KW - Toll-Like Receptors KW - Tumor Necrosis Factor-alpha KW - Index Medicus KW - Receptors, Cell Surface -- metabolism KW - Animals KW - Humans KW - Signal Transduction -- drug effects KW - Gene Expression Regulation KW - Cytokines -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Free Radicals -- metabolism KW - Drug Design KW - Receptors, Tumor Necrosis Factor, Type II -- metabolism KW - Membrane Glycoproteins -- metabolism KW - Neoplasms -- drug therapy KW - Inflammation -- physiopathology KW - Neoplasms -- pathology KW - Inflammation -- etiology KW - Inflammation -- metabolism KW - Antineoplastic Agents -- therapeutic use KW - Antineoplastic Agents -- pharmacology KW - Neoplasms -- etiology KW - NF-kappa B -- genetics KW - NF-kappa B -- metabolism KW - NF-kappa B -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68479663?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+cancer+drug+targets&rft.atitle=Inflammation+and+cancer%3A+when+NF-kappaB+amalgamates+the+perilous+partnership.&rft.au=Dobrovolskaia%2C+Marina+A%3BKozlov%2C+Serguei+V&rft.aulast=Dobrovolskaia&rft.aufirst=Marina&rft.date=2005-08-01&rft.volume=5&rft.issue=5&rft.spage=325&rft.isbn=&rft.btitle=&rft.title=Current+cancer+drug+targets&rft.issn=15680096&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - First- and second-generation antipsychotic medication and cognitive processing in schizophrenia. AN - 68478870; 16098285 AB - Schizophrenia has been consistently characterized by deficits in the cognitive domains of executive function, working memory, attention, and episodic memory. Although some cognitive abnormalities, such as motor slowing, may be associated with antipsychotic medication administration, generally the cognitive deficits shown by patients with schizophrenia can be attributed at least in part to the disease process. Modulation of the dopamine neurotransmitter system, notably through D2 receptor blockade, has been associated with psychotic symptom reduction and cognitive performance improvements in patients with schizophrenia. Although first-generation antipsychotic medication treatment initially was thought not to result in cognitive improvement, recent studies comparing second-generation antipsychotics to low doses of first-generation antipsychotic medication showed cognitive benefits for first-generation drugs, although perhaps not as great as that found after treatment with second-generation medication. Cognitive improvement associated with administration of antipsychotic medication may be a manifestation of improvement in general cortical information processing. Recent work has shown that specific genetic polymorphisms may interact with antipsychotic medication treatment to influence the degree to which cognitive abilities display improvement after treatment. In particular, the catechol-O-methyltransferase val108/158met polymorphism has been shown to predict working memory improvement after administration of antipsychotic medication to patients with schizophrenia. JF - Current psychiatry reports AU - Weickert, Thomas W AU - Goldberg, Terry E AD - Clinical Brain Disorders Branch, Genes, Cognition, and Psychosis Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 304 EP - 310 VL - 7 IS - 4 SN - 1523-3812, 1523-3812 KW - Antipsychotic Agents KW - 0 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Genotype KW - Psychomotor Performance -- drug effects KW - Polymorphism, Genetic -- genetics KW - Humans KW - Dopamine -- metabolism KW - Reaction Time -- drug effects KW - Cognition -- drug effects KW - Antipsychotic Agents -- therapeutic use KW - Antipsychotic Agents -- pharmacology KW - Cognition Disorders -- genetics KW - Schizophrenia -- drug therapy KW - Schizophrenia -- genetics KW - Antipsychotic Agents -- adverse effects KW - Schizophrenia -- complications KW - Cognition Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68478870?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+psychiatry+reports&rft.atitle=First-+and+second-generation+antipsychotic+medication+and+cognitive+processing+in+schizophrenia.&rft.au=Weickert%2C+Thomas+W%3BGoldberg%2C+Terry+E&rft.aulast=Weickert&rft.aufirst=Thomas&rft.date=2005-08-01&rft.volume=7&rft.issue=4&rft.spage=304&rft.isbn=&rft.btitle=&rft.title=Current+psychiatry+reports&rft.issn=15233812&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-12 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Bladder cancer screening and monitoring of 4,4'-methylenebis(2-chloroaniline) exposure among workers in Taiwan. AN - 68475838; 16098360 AB - Transitional cell carcinoma of the urinary bladder is associated with occupational exposure to 4,4'-methylenebis(2-chloroaniline) (MBOCA). A program to monitor MBOCA levels in the work environment and to screen for bladder cancer was performed at four MBOCA manufacturing factories. The U.S. Occupational Safety and Health Administration analytic method No. 24 was adopted in this study to measure air MBOCA concentrations. A total of 70 MBOCA-exposed workers and another 92 nonexposed workers were recruited for screening. Urine occult blood tests, urine cytology, tests for the urine tumor marker nuclear matrix protein, and abdominal ultrasonography were performed in all patients. Intravenous urography and cystoscopy were used to confirm the presence of bladder cancer. The air concentration of MBOCA was greatest in the purification area (0.23 to 0.41 mg/m3), followed by the washing area (less than 0.02 to 0.08 mg/m3) and neutralization area (less than 0.05 to 0.06 mg/m3). This study identified a current worker with proved bladder cancer. In addition, we also identified 1 worker with suspected malignant cells on urine cytology and 1 worker with atypical cytology combined with gross hematuria. Although the prevalence of atypical urinary cells and the nuclear matrix protein 22 tumor marker was not significantly different between the MBOCA-exposed workers and nonexposed workers as a whole or when grouped by sex, the prevalence of positive occult blood was marginally significantly (P = 0.055) greater in male exposed workers (18%) than in male nonexposed workers (7%). The findings of this study support the conclusions from other studies that MBOCA is potentially carcinogenic to humans. Control measures are needed to prevent overexposure from inhalation and skin absorption. JF - Urology AU - Chen, Hong-I AU - Liou, Saou-Hsing AU - Loh, Ching-Hui AU - Uang, Shi-Nian AU - Yu, Yi-Chun AU - Shih, Tung-Sheng AD - Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Nei-Hu, Taipei, Taiwan, Republic of China. hong_i@ndmctsgh.edu.tw Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 305 EP - 310 VL - 66 IS - 2 KW - Methylenebis(chloroaniline) KW - 3L2W5VTT2A KW - Index Medicus KW - Mass Screening KW - Taiwan KW - Humans KW - Adult KW - Male KW - Female KW - Occupational Exposure KW - Occupational Diseases -- diagnosis KW - Urinary Bladder Neoplasms -- diagnosis KW - Carcinoma, Transitional Cell -- diagnosis KW - Carcinoma, Transitional Cell -- chemically induced KW - Occupational Diseases -- chemically induced KW - Urinary Bladder Neoplasms -- chemically induced KW - Methylenebis(chloroaniline) -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68475838?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Urology&rft.atitle=Bladder+cancer+screening+and+monitoring+of+4%2C4%27-methylenebis%282-chloroaniline%29+exposure+among+workers+in+Taiwan.&rft.au=Chen%2C+Hong-I%3BLiou%2C+Saou-Hsing%3BLoh%2C+Ching-Hui%3BUang%2C+Shi-Nian%3BYu%2C+Yi-Chun%3BShih%2C+Tung-Sheng&rft.aulast=Chen&rft.aufirst=Hong-I&rft.date=2005-08-01&rft.volume=66&rft.issue=2&rft.spage=305&rft.isbn=&rft.btitle=&rft.title=Urology&rft.issn=1527-9995&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-28 N1 - Date created - 2005-08-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Diagnosis and management of primary intraocular lymphoma. AN - 68461233; 16083834 AB - Primary intraocular lymphoma (PIOL) is a subset of primary central nervous system lymphoma. The incidence of PIOL has increased in the past 20 years. PIOL often presents as chronic uveitis that is resistant to corticosteroid therapy. Diagnosing PIOL can be challenging and requires an expert pathologist. The treatment of PIOL is difficult because of its high recurrence rate and refractory nature. The objective for the future is to improve diagnostic techniques and therapeutic success while minimizing ocular toxicities. JF - Hematology/oncology clinics of North America AU - Levy-Clarke, Grace A AU - Chan, Chi-Chao AU - Nussenblatt, Robert B AD - Laboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 10N112, Bethesda, MD 20892, USA. clarkeg@nei.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 739 EP - 49, viii VL - 19 IS - 4 SN - 0889-8588, 0889-8588 KW - Index Medicus KW - Humans KW - Lymphoma -- therapy KW - Eye Neoplasms -- therapy KW - Eye Neoplasms -- diagnosis KW - Lymphoma -- diagnosis KW - Central Nervous System Neoplasms -- therapy KW - Lymphoma -- genetics KW - Central Nervous System Neoplasms -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68461233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Hematology%2Foncology+clinics+of+North+America&rft.atitle=Diagnosis+and+management+of+primary+intraocular+lymphoma.&rft.au=Levy-Clarke%2C+Grace+A%3BChan%2C+Chi-Chao%3BNussenblatt%2C+Robert+B&rft.aulast=Levy-Clarke&rft.aufirst=Grace&rft.date=2005-08-01&rft.volume=19&rft.issue=4&rft.spage=739&rft.isbn=&rft.btitle=&rft.title=Hematology%2Foncology+clinics+of+North+America&rft.issn=08898588&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate. AN - 68456002; 16084394 AB - DNA polymerases occasionally insert the wrong nucleotide. For this error to become a mutation, the mispair must be extended. We report a structure of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of the polymerase active site. The structure of this complex indicates that the templating adenine of the mispair stacks with the primer terminus adenine while the templating (coding) cytosine is flipped out of the DNA helix. Soaking the crystals of the binary complex with dGTP resulted in crystals of a ternary substrate complex. In this case, the templating cytosine is observed within the DNA helix and forms Watson-Crick hydrogen bonds with the incoming dGTP. The adenine at the primer terminus has rotated into a syn-conformation to interact with the opposite adenine in a planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out of position for efficient nucleotide insertion. JF - Structure (London, England : 1993) AU - Batra, Vinod K AU - Beard, William A AU - Shock, David D AU - Pedersen, Lars C AU - Wilson, Samuel H AD - Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1225 EP - 1233 VL - 13 IS - 8 SN - 0969-2126, 0969-2126 KW - Nucleotides KW - 0 KW - DNA KW - 9007-49-2 KW - DNA Polymerase beta KW - EC 2.7.7.- KW - Index Medicus KW - Humans KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Mutation KW - Nucleotides -- metabolism KW - DNA -- metabolism KW - DNA Polymerase beta -- chemistry KW - DNA Polymerase beta -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68456002?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Structure+%28London%2C+England+%3A+1993%29&rft.atitle=Nucleotide-induced+DNA+polymerase+active+site+motions+accommodating+a+mutagenic+DNA+intermediate.&rft.au=Batra%2C+Vinod+K%3BBeard%2C+William+A%3BShock%2C+David+D%3BPedersen%2C+Lars+C%3BWilson%2C+Samuel+H&rft.aulast=Batra&rft.aufirst=Vinod&rft.date=2005-08-01&rft.volume=13&rft.issue=8&rft.spage=1225&rft.isbn=&rft.btitle=&rft.title=Structure+%28London%2C+England+%3A+1993%29&rft.issn=09692126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-19 N1 - Date created - 2005-08-08 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1ZJM; PDB; 1ZJN N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long-term effects of neonatal exposure to hydroxylated polychlorinated biphenyls in the BALB/cCrgl mouse. AN - 68446905; 16079073 AB - The neonatal mouse model has been a valuable tool in determining the long-term effects of early exposure to estrogenic agents in mammals. Using this model, we compared the effects of 2',4',6'-trichloro-4-biphenylol (OH-PCB-30) and 2',3',4',5'-tetrachloro-4-biphenylol (OH-PCB-61) as prototype estrogenic hydroxylated PCBs (OH-PCBs) because they are reported to exhibit relatively high estrogenic activity both in vivo and in vitro. The purpose of this study was to examine the relationship between estrogenicity and carcinogenicity of OH-PCB congeners. The OH-PCBs were tested individually and in combination to determine whether effects of combined OH-PCBs differed from those of these OH-PCBs alone. We evaluated the long-term effects of neonatal exposure to OH-PCBs with treatment doses that were based on the reported binding affinity of specific OH-PCB congeners to estrogen receptor alpha. BALB/cCrgl female mice were treated within 16 hr after birth by subcutaneous injections every 24 hr, for 5 days. The mice treated with OH-PCB-30 (200 microg/day) or 17beta-estradiol (5 microg/day) showed similar increased incidences of cervicovaginal (CV) tract carcinomas (43% and 47%, respectively). In addition, when mice were treated with OH-PCBs as a mixture, a change in the type of CV tract tumor was observed, shifting from predominantly squamous cell carcinomas to adenosquamous cell carcinoma. From our results, we conclude that the individual OH-PCBs tested were estrogenic and tumorigenic in mice when exposed during development of the reproductive tract. These data support the hypothesis that mixtures may act differently and unexpectedly than do individual compounds. JF - Environmental health perspectives AU - Martinez, Jeanelle M AU - Stephens, L Clifton AU - Jones, Lovell A AD - Department of Gynecologic Oncology, University of Texas, M.D. Anderson Cancer Center, Houston, Texas, USA. martine2@niehs.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1022 EP - 1026 VL - 113 IS - 8 SN - 0091-6765, 0091-6765 KW - Environmental Pollutants KW - 0 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Animals, Newborn KW - Animals KW - Environmental Pollutants -- toxicity KW - Mice KW - Mice, Inbred BALB C KW - Female KW - Hydroxylation KW - Vaginal Neoplasms -- chemically induced KW - Polychlorinated Biphenyls -- toxicity KW - Carcinoma, Squamous Cell -- chemically induced KW - Carcinoma, Adenosquamous -- chemically induced KW - Uterine Cervical Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68446905?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Long-term+effects+of+neonatal+exposure+to+hydroxylated+polychlorinated+biphenyls+in+the+BALB%2FcCrgl+mouse.&rft.au=Martinez%2C+Jeanelle+M%3BStephens%2C+L+Clifton%3BJones%2C+Lovell+A&rft.aulast=Martinez&rft.aufirst=Jeanelle&rft.date=2005-08-01&rft.volume=113&rft.issue=8&rft.spage=1022&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-17 N1 - Date created - 2005-08-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 1999 Jan;107(1):45-51 [9872716] Chemosphere. 1998 Oct-Nov;37(9-12):1845-53 [9828313] Toxicol Lett. 1998 Dec 28;102-103:331-5 [10022274] Environ Health Perspect. 1999 Aug;107 Suppl 4:639-49 [10421775] Sci Total Environ. 1999 Aug 15;233(1-3):141-61 [10492903] J Natl Cancer Inst. 1963 Aug;31:425-55 [14046632] Toxicology. 2005 Mar 30;208(3):377-87 [15695023] Toxicol Sci. 2005 Mar;84(1):49-62 [15601674] J Natl Cancer Inst. 1976 Nov;57(5):1057-62 [187788] J Natl Cancer Inst. 1976 Nov;57(5):1185-9 [187796] Arch Pathol Lab Med. 1977 Jan;101(1):1-5 [576195] Environ Res. 1978 Jul;16(1-3):123-30 [98321] Arch Toxicol. 1978 Dec 28;41(3):179-86 [104695] Cancer Res. 1979 Jul;39(7 Pt 1):2560-7 [445458] J Pediatr. 1984 Aug;105(2):315-20 [6431068] Arch Environ Contam Toxicol. 1985 Jan;14(1):51-7 [3919656] Environ Health Perspect. 1985 May;60:35-9 [2992924] IARC Sci Publ. 1985;(65):107-17 [4086078] Environ Res. 1986 Oct;41(1):14-22 [3093216] Am J Pathol. 2000 Apr;156(4):1289-98 [10751354] Int J Cancer. 2001 Feb 15;91(4):568-74 [11251983] Environ Toxicol Chem. 2001 Feb;20(2):351-8 [11351435] Environ Health Perspect. 2001 Jul;109(7):739-47 [11485874] Arch Environ Contam Toxicol. 2002 Jan;42(1):105-17 [11706375] Cancer Epidemiol Biomarkers Prev. 2002 Dec;11(12):1560-5 [12496044] Environ Sci Technol. 2003 Mar 1;37(5):832-9 [12666909] Environ Health Perspect. 2003 Apr;111(4):389-94 [12676588] Cancer. 2003 May 1;97(9):2196-202 [12712471] Environ Health Perspect. 2004 Aug;112(11):1208-12 [15289169] N Engl J Med. 1971 Apr 15;284(15):878-81 [5549830] Res Commun Chem Pathol Pharmacol. 1974 Sep;9(1):85-95 [4216060] J Steroid Biochem. 1975 May;6(5):673-6 [1186250] Cancer Res. 1977 Jan;37(1):67-75 [830422] Chem Biol Interact. 1986 Sep;59(2):173-84 [3769051] Mol Pharmacol. 1988 Jan;33(1):120-6 [3122017] Arch Environ Health. 1987 Nov-Dec;42(6):333-9 [3125795] Am J Epidemiol. 1989 Feb;129(2):395-406 [2492144] Cancer Res. 1990 Dec 1;50(23):7677-81 [2174729] J Natl Cancer Inst. 1994 Apr 20;86(8):589-99 [8145274] J Cardiovasc Surg (Torino). 1994 Dec;35(6):543-7 [7698972] Early Hum Dev. 1995 Apr 14;41(2):111-27 [7601016] Chemosphere. 1995 Aug;31(4):3017-23 [7552046] Arch Environ Contam Toxicol. 1995 Oct;29(3):334-43 [7487157] Environ Health Perspect. 1994 May;102(5):464-9 [8593850] Environ Health Perspect. 1995 Sep;103 Suppl 6:117-22 [8549457] Cancer Causes Control. 1995 Nov;6(6):551-66 [8580305] Environ Health Perspect. 1995 Oct;103 Suppl 7:141-5 [8593861] Biol Reprod. 1997 May;56(5):1147-57 [9160713] Environ Health Perspect. 1997 Apr;105 Suppl 3:577-81 [9167998] Toxicol Appl Pharmacol. 1997 Jul;145(1):111-23 [9221830] Environ Health Perspect. 1997 Oct;105(10):1030-2 [9349835] Toxicol Appl Pharmacol. 1997 Nov;147(1):93-100 [9356311] Ecotoxicol Environ Saf. 1997 Dec;38(3):281-5 [9469881] Toxicol Sci. 1998 Jan;41(1):62-76 [9520342] Chemosphere. 1997 Feb;34(4):835-48 [9569946] Am J Ind Med. 1998 Jul;34(1):6-14 [9617382] J Pediatr. 1999 Jan;134(1):33-41 [9880446] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Strategic planning: establishing need and clarifying motivation. AN - 68444424; 16079048 JF - Environmental health perspectives AU - Wilson, Samuel H AU - Schwartz, David A AD - wilson5@niehs.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1 VL - 113 IS - 8 SN - 0091-6765, 0091-6765 KW - Index Medicus KW - United States KW - Environmental Exposure KW - Organizational Objectives KW - Research KW - Planning Techniques KW - Environmental Health KW - National Institutes of Health (U.S.) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68444424?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Strategic+planning%3A+establishing+need+and+clarifying+motivation.&rft.au=Wilson%2C+Samuel+H%3BSchwartz%2C+David+A&rft.aulast=Wilson&rft.aufirst=Samuel&rft.date=2005-08-01&rft.volume=113&rft.issue=8&rft.spage=A506&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-17 N1 - Date created - 2005-08-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis. AN - 68441695; 16061934 AB - Zebrafish has become a favorite model organism not only in genetics and developmental biology, but also for the study of cancer, neuroscience and metabolism. However, strategies for reverse genetics in zebrafish are mostly limited to the use of antisense oligonucleotides, and therefore the development of other targeting methods is highly desirable. Here, we report an approach to gene targeting in this system in which single-stranded oligonucleotides and zebrafish Rad52 protein are employed. It has been proposed that a single-stranded oligonucleotide containing a mutation can be incorporated into the genome by annealing to the single-stranded region of the lagging strand of the replication fork. Rad52 is expected to accelerate the annealing step. In vitro experiments using purified truncated Rad52 proteins and replication protein A (RPA) showed that annealing of oligonucleotides is accelerated by Rad52 in the presence of RPA. We developed a simple and sensitive PCR-based method to detect point mutations in the genome. In exploratory experiments, we found that microinjection of single-stranded oligonucleotide targeted to a specific gene together with truncated Rad52 into zebrafish embryos resulted in a low level of recombinant copies in 3 of the 80 embryos tested under these conditions. JF - Nucleic acids research AU - Takahashi, Nobuhiro AU - Dawid, Igor B AD - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD 20892-2790, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1 VL - 33 IS - 13 KW - DNA-Binding Proteins KW - 0 KW - Nuclear Localization Signals KW - Oligonucleotides KW - Rad52 DNA Repair and Recombination Protein KW - Rad52 protein, zebrafish KW - Replication Protein A KW - Zebrafish Proteins KW - Index Medicus KW - Polymerase Chain Reaction KW - Animals KW - Sequence Alignment KW - Cells, Cultured KW - Point Mutation KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Microinjections KW - Cloning, Molecular KW - Mutagenesis, Site-Directed KW - Zebrafish Proteins -- isolation & purification KW - Oligonucleotides -- chemistry KW - DNA-Binding Proteins -- genetics KW - Zebrafish Proteins -- genetics KW - DNA-Binding Proteins -- isolation & purification KW - Zebrafish -- genetics KW - Zebrafish -- embryology KW - Oligonucleotides -- metabolism KW - Oligonucleotides -- administration & dosage KW - Zebrafish Proteins -- metabolism KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68441695?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+research&rft.atitle=Characterization+of+zebrafish+Rad52+and+replication+protein+A+for+oligonucleotide-mediated+mutagenesis.&rft.au=Takahashi%2C+Nobuhiro%3BDawid%2C+Igor+B&rft.aulast=Takahashi&rft.aufirst=Nobuhiro&rft.date=2005-08-01&rft.volume=33&rft.issue=13&rft.spage=e120&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+research&rft.issn=1362-4962&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - DQ021478; GENBANK; DQ021477 N1 - SuppNotes - Cited By: Mol Cell. 2002 Aug;10(2):359-71 [12191481] Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13492-7 [12370410] J Mol Med (Berl). 2002 Dec;80(12):770-81 [12483462] Mar Biotechnol (NY). 2003 Mar-Apr;5(2):174-84 [12876654] Nucleic Acids Res. 2003 Nov 15;31(22):6674-87 [14602928] Nucleic Acids Res. 2004;32(7):2093-101 [15087488] Curr Mol Med. 2004 Aug;4(5):445-63 [15267219] Mol Cell Biol. 1987 Jul;7(7):2329-34 [3302673] Proc Natl Acad Sci U S A. 1988 Jan;85(2):524-8 [2829192] Nucleic Acids Res. 1990 Feb 25;18(4):999-1005 [2179874] Mol Cell Biol. 1992 Jul;12(7):3050-9 [1320195] Genetics. 1992 Aug;131(4):811-9 [1325385] J Mol Biol. 1992 Sep 5;227(1):54-71 [1522601] Yeast. 1992 Nov;8(11):935-48 [1336288] J Biol Chem. 1994 Apr 15;269(15):11121-32 [8157639] Dev Dyn. 1995 Jul;203(3):253-310 [8589427] Nature. 1997 Jan 9;385(6612):176-81 [8990123] Development. 1996 Dec;123:1-36 [9007226] Proc Natl Acad Sci U S A. 1998 May 26;95(11):6049-54 [9600915] Genes Cells. 1998 Mar;3(3):145-56 [9619627] Annu Rev Biochem. 1998;67:721-51 [9759502] Genes Dev. 2004 Nov 15;18(22):2764-73 [15520275] Methods Cell Biol. 2004;76:151-60 [15602876] Methods Cell Biol. 2004;76:593-612 [15602894] Methods Cell Biol. 2004;77:69-90 [15602906] Methods Cell Biol. 2004;77:91-112 [15602907] Methods Cell Biol. 2004;77:113-9 [15602908] Methods Cell Biol. 2004;77:121-36 [15602909] Mech Dev. 2000 May;93(1-2):205-9 [10781958] Cell. 2000 Oct 27;103(3):449-56 [11081631] Gene Ther. 2001 Mar;8(5):391-9 [11313816] Nucleic Acids Res. 2001 Oct 15;29(20):4238-50 [11600713] Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14298-303 [11724925] Sci STKE. 2001 Mar 13;2001(73):pl1 [11752645] Mol Cell Biol. 2002 Jun;22(11):3852-63 [11997519] Methods Cell Biol. 2004;77:137-58 [15602910] Methods Cell Biol. 2004;77:403-11 [15602924] Nat Methods. 2004 Nov;1(2):141-7 [15782177] Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6742-6 [11381128] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long-latency afferent inhibition during selective finger movement. AN - 68439280; 15843479 AB - Stimulation of a peripheral nerve of a hand at rest modulates excitability in the motor cortex and, in particular, leads to inhibition when applied at an interval of approximately 200 ms (long-latency afferent inhibition; LAI). Surround inhibition (SI) is the process that inhibits neighboring muscles not involved in a particular task. The neuronal mechanisms of SI are not known, and it is possible that LAI might contribute to it. Using transcranial magnetic stimulation (TMS) with and without movement of the index finger, the motor-evoked potentials (MEPs) were measured of two functionally distinct target muscles of the hand (abductor digiti minimi muscle = ADM, 1st dorsal interosseus muscle = FDI). Electrical stimulation was applied 180 ms before TMS to either the fifth finger or the index finger. Both homotopic and heterotopic finger stimulation resulted in LAI without movement. With index finger movement, motor output further decreased with homo- and heterotopic stimulation in the ADM. In the moving FDI, however, there was no change with either homo- or heterotopic stimulation. Additionally, in the unstimulated movement trials, LAI increased with the amount of unintentional co-activation that occurred despite attempts to maintain the ADM at rest. However, with finger stimulation added, there were almost no increased MEPs despite co-activation. These findings suggest that LAI increases during movement and can enhance SI. JF - Journal of neurophysiology AU - Voller, Bernhard AU - St Clair Gibson, Alan AU - Lomarev, Mikhail AU - Kanchana, Sulada AU - Dambrosia, James AU - Dang, Nguyet AU - Hallett, Mark AD - Human Motor Control Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1115 EP - 1119 VL - 94 IS - 2 SN - 0022-3077, 0022-3077 KW - Index Medicus KW - Electric Stimulation -- methods KW - Analysis of Variance KW - Magnetics KW - Evoked Potentials -- radiation effects KW - Humans KW - Electromyography -- methods KW - Adult KW - Evoked Potentials -- physiology KW - Middle Aged KW - Dose-Response Relationship, Radiation KW - Time Factors KW - Male KW - Female KW - Reaction Time -- radiation effects KW - Afferent Pathways -- radiation effects KW - Neural Inhibition -- radiation effects KW - Movement -- physiology KW - Fingers -- physiology KW - Afferent Pathways -- physiology KW - Neural Inhibition -- physiology KW - Reaction Time -- physiology KW - Movement -- radiation effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68439280?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Long-latency+afferent+inhibition+during+selective+finger+movement.&rft.au=Voller%2C+Bernhard%3BSt+Clair+Gibson%2C+Alan%3BLomarev%2C+Mikhail%3BKanchana%2C+Sulada%3BDambrosia%2C+James%3BDang%2C+Nguyet%3BHallett%2C+Mark&rft.aulast=Voller&rft.aufirst=Bernhard&rft.date=2005-08-01&rft.volume=94&rft.issue=2&rft.spage=1115&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Presynaptic angiotensin II AT1 receptors enhance inhibitory and excitatory synaptic neurotransmission to motoneurons and other ventral horn neurons in neonatal rat spinal cord. AN - 68438130; 16061493 AB - In neonatal spinal cord, we previously reported that exogenous angiotensin II (ANG II) acts at postsynaptic AT1 receptors to depolarize neonatal rat spinal ventral horn neurons in vitro. This study evaluated an associated increase in synaptic activity. Patch clamp recordings revealed that 38/81 thoracolumbar (T7-L5) motoneurons responded to bath applied ANG II (0.3-1 microM; 30 s) with a prolonged (5-10 min) and reversible increase in spontaneous postsynaptic activity, selectively blockable with Losartan (n = 5) but not PD123319 (n = 5). ANG-II-induced events included both spontaneous inhibitory (IPSCs; n = 6) and excitatory postsynaptic currents (EPSCs; n = 5). While most ANG induced events were tetrodotoxin-sensitive, ANG induced a significant tetrodotoxin-resistant increase in frequency but not amplitude of miniature IPSCs (n = 7/13 cells) and EPSCs (n = 2/7 cells). In 35/77 unidentified neurons, ANG II also induced a tetrodotoxin-sensitive and prolonged increase in their spontaneous synaptic activity that featured both IPSCs (n = 5) and EPSCs (n = 4) when tested in the presence of selective amino acid receptor antagonists. When tested in the presence of tetrodotoxin, ANG II was noted to induce a significant increase in the frequency but not the amplitude of mIPSCs (n = 9) and mEPSCs (n = 8). ANG also increased spontaneous motor activity from isolated mouse lumbar ventral rootlets. Collectively, these observations support the existence of a wide pre- and postsynaptic distribution of ANG II AT1 receptors in neonatal ventral spinal cord that are capable of influencing both inhibitory and excitatory neurotransmission. JF - Journal of neurophysiology AU - Oz, Murat AU - Yang, Keun-Hang AU - O'donovan, Michael J AU - Renaud, Leo P AD - National Institute on Drug Abuse, Cellular Neurobiology Branch, National Institutes of Health, Baltimore, MD 21224, USA. moz@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1405 EP - 1412 VL - 94 IS - 2 SN - 0022-3077, 0022-3077 KW - Excitatory Amino Acid Antagonists KW - 0 KW - GABA Antagonists KW - Quinoxalines KW - Receptor, Angiotensin, Type 1 KW - Angiotensin II KW - 11128-99-7 KW - 2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline KW - 118876-58-7 KW - Tetrodotoxin KW - 4368-28-9 KW - 2-Amino-5-phosphonovalerate KW - 76726-92-6 KW - Strychnine KW - H9Y79VD43J KW - Bicuculline KW - Y37615DVKC KW - Index Medicus KW - Patch-Clamp Techniques -- methods KW - Bicuculline -- pharmacology KW - Animals KW - Drug Interactions KW - Electric Stimulation -- methods KW - Dose-Response Relationship, Drug KW - GABA Antagonists -- pharmacology KW - Angiotensin II -- pharmacology KW - Excitatory Amino Acid Antagonists -- pharmacology KW - Rats KW - Animals, Newborn KW - Rats, Sprague-Dawley KW - Laminectomy -- methods KW - 2-Amino-5-phosphonovalerate -- pharmacology KW - In Vitro Techniques KW - Strychnine -- pharmacology KW - Tetrodotoxin -- pharmacology KW - Quinoxalines -- pharmacology KW - Male KW - Female KW - Motor Neurons -- physiology KW - Excitatory Postsynaptic Potentials -- drug effects KW - Motor Neurons -- classification KW - Receptor, Angiotensin, Type 1 -- physiology KW - Motor Neurons -- cytology KW - Neural Inhibition -- drug effects KW - Synaptic Transmission -- physiology KW - Neural Inhibition -- physiology KW - Excitatory Postsynaptic Potentials -- physiology KW - Spinal Cord -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68438130?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Presynaptic+angiotensin+II+AT1+receptors+enhance+inhibitory+and+excitatory+synaptic+neurotransmission+to+motoneurons+and+other+ventral+horn+neurons+in+neonatal+rat+spinal+cord.&rft.au=Oz%2C+Murat%3BYang%2C+Keun-Hang%3BO%27donovan%2C+Michael+J%3BRenaud%2C+Leo+P&rft.aulast=Oz&rft.aufirst=Murat&rft.date=2005-08-01&rft.volume=94&rft.issue=2&rft.spage=1405&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. AN - 68437118; 16041368 AB - Centrosome duplication is tightly controlled during faithful cell division, and unnecessary reduplication can lead to supernumerary centrosomes and multipolar spindles that are associated with most human cancer cells. In addition to nucleocytoplasmic transport, the Ran-Crm1 network is involved in regulating centrosome duplication to ensure the formation of a bipolar spindle. Here, we discover that nucleophosmin (NPM) may be a Ran-Crm1 substrate that controls centrosome duplication. NPM contains a functional nuclear export signal (NES) that is responsible for both its nucleocytoplasmic shuttling and its association with centrosomes, which are Ran-Crm1-dependent as they are sensitive to Crm1-specific nuclear export inhibition, either by leptomycin B (LMB) or by the expression of a Ran-binding protein, RanBP1. Notably, LMB treatment induces premature centrosome duplication in quiescent cells, which coincides with NPM dissociation from centrosomes. Moreover, deficiency of NPM by RNA interference results in supernumerary centrosomes, which can be reversed by reintroducing wild-type but not NES-mutated NPM. Mutation of a potential proline-dependent kinase phosphorylation site at residue 95, from threonine to aspartic acid (T95D) within the NES motif, abolishes NPM association and inhibition of centrosome duplication. Our results are consistent with the hypothesis that the Ran-Crm1 complex may promote a local enrichment of NPM on centrosomes, thereby preventing centrosome reduplication. JF - Nature cell biology AU - Wang, Wei AU - Budhu, Anuradha AU - Forgues, Marshonna AU - Wang, Xin Wei AD - Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute/NIH, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 823 EP - 830 VL - 7 IS - 8 SN - 1465-7392, 1465-7392 KW - Fatty Acids, Unsaturated KW - 0 KW - Karyopherins KW - Nuclear Proteins KW - RNA, Small Interfering KW - Receptors, Cytoplasmic and Nuclear KW - Trans-Activators KW - exportin 1 protein KW - hepatitis B virus X protein KW - ran-binding protein 1 KW - nucleophosmin KW - 117896-08-9 KW - Threonine KW - 2ZD004190S KW - ran GTP-Binding Protein KW - EC 3.6.5.2 KW - leptomycin B KW - Y031I2N1EO KW - Index Medicus KW - Animals KW - Threonine -- metabolism KW - Threonine -- genetics KW - Cell Nucleus -- metabolism KW - Humans KW - Interphase -- physiology KW - Protein Binding -- physiology KW - Phosphorylation KW - Trans-Activators -- genetics KW - Molecular Sequence Data KW - Binding Sites -- genetics KW - Sequence Homology, Amino Acid KW - Protein Transport -- physiology KW - HeLa Cells KW - Active Transport, Cell Nucleus -- drug effects KW - Protein Transport -- drug effects KW - Amino Acid Sequence KW - Mice KW - RNA, Small Interfering -- genetics KW - Binding Sites -- physiology KW - Gene Expression -- genetics KW - Cell Fusion KW - Transfection KW - Active Transport, Cell Nucleus -- physiology KW - Mutation -- genetics KW - Fatty Acids, Unsaturated -- pharmacology KW - Cell Line KW - Receptors, Cytoplasmic and Nuclear -- physiology KW - Nuclear Proteins -- genetics KW - Karyopherins -- physiology KW - ran GTP-Binding Protein -- genetics KW - ran GTP-Binding Protein -- physiology KW - Nuclear Proteins -- metabolism KW - Nuclear Proteins -- physiology KW - Centrosome -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68437118?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+cell+biology&rft.atitle=Temporal+and+spatial+control+of+nucleophosmin+by+the+Ran-Crm1+complex+in+centrosome+duplication.&rft.au=Wang%2C+Wei%3BBudhu%2C+Anuradha%3BForgues%2C+Marshonna%3BWang%2C+Xin+Wei&rft.aulast=Wang&rft.aufirst=Wei&rft.date=2005-08-01&rft.volume=7&rft.issue=8&rft.spage=823&rft.isbn=&rft.btitle=&rft.title=Nature+cell+biology&rft.issn=14657392&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A new direction for gene therapy: intrathymic T cell-specific lentiviral gene transfer. AN - 68436682; 16075048 AB - Reports of neoplasia related to insertional activation of protooncogenes by retroviral vectors have raised serious safety concerns in the field of gene therapy. Modification of current approaches is urgently required to minimize the deleterious consequences of insertional mutagenesis. In this issue of the JCI, Adjali and colleagues report on their treatment of SCID mice lacking the 70-kDa protein tyrosine kinase, ZAP-70, with direct intrathymic injection of a ZAP-70-expressing T cell-specific lentiviral vector, which resulted in T cell reconstitution. Using lentiviral vectors and in situ gene transfer may represent a safer approach than using retroviral vectors for ex vivo gene transfer into HSCs, avoiding 3 factors potentially linked to leukemogenesis, namely HSC targets, ex vivo transduction and expansion, and standard Moloney leukemia virus-based retroviral vectors. JF - The Journal of clinical investigation AU - Seggewiss, Ruth AU - Dunbar, Cynthia E AD - National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 2064 EP - 2067 VL - 115 IS - 8 SN - 0021-9738, 0021-9738 KW - Protein-Tyrosine Kinases KW - EC 2.7.10.1 KW - ZAP-70 Protein-Tyrosine Kinase KW - EC 2.7.10.2 KW - ZAP70 protein, human KW - Zap70 protein, mouse KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Humans KW - Mice KW - Mice, SCID KW - Neoplasms -- genetics KW - Protein-Tyrosine Kinases -- genetics KW - Severe Combined Immunodeficiency -- enzymology KW - Genetic Therapy -- adverse effects KW - Severe Combined Immunodeficiency -- therapy KW - Lentivirus KW - Genetic Therapy -- methods KW - Thymus Gland -- enzymology KW - Protein-Tyrosine Kinases -- metabolism KW - T-Lymphocytes -- enzymology KW - Severe Combined Immunodeficiency -- genetics KW - Transduction, Genetic -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68436682?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+clinical+investigation&rft.atitle=A+new+direction+for+gene+therapy%3A+intrathymic+T+cell-specific+lentiviral+gene+transfer.&rft.au=Seggewiss%2C+Ruth%3BDunbar%2C+Cynthia+E&rft.aulast=Seggewiss&rft.aufirst=Ruth&rft.date=2005-08-01&rft.volume=115&rft.issue=8&rft.spage=2064&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+clinical+investigation&rft.issn=00219738&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: N Engl J Med. 2000 May 4;342(18):1325-32 [10793165] BMC Immunol. 2004 Aug 19;5:18 [15318949] Mol Ther. 2000 Mar;1(3):285-93 [10933944] Science. 2000 Apr 28;288(5466):669-72 [10784449] J Immunol. 1994 May 15;152(10):4758-66 [8176201] N Engl J Med. 1996 Nov 21;335(21):1563-7 [8900089] J Pediatr. 1997 Mar;130(3):378-87 [9063412] Immunity. 1997 Jun;6(6):663-71 [9208839] J Exp Med. 1998 Jul 20;188(2):393-8 [9670051] Semin Hematol. 1998 Oct;35(4):310-20 [9801260] PLoS Biol. 2004 Dec;2(12):e423 [15550989] J Clin Invest. 2005 Aug;115(8):2287-95 [16075064] J Clin Invest. 2001 Aug;108(3):447-55 [11489938] Blood. 2002 Feb 15;99(4):1165-73 [11830462] N Engl J Med. 2002 Apr 18;346(16):1185-93 [11961146] Cell. 2002 Aug 23;110(4):521-9 [12202041] Lancet. 2003 Feb 15;361(9357):553-60 [12598139] Science. 2003 Oct 17;302(5644):415-9 [14564000] Annu Rev Immunol. 2004;22:625-55 [15032591] Comment On: J Clin Invest. 2005 Aug;115(8):2287-95 [16075064] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Increased tumorigenicity and sensitivity to ionizing radiation upon loss of chromosomal protein HMGN1. AN - 68435942; 16061652 AB - We report that loss of HMGN1, a nucleosome-binding protein that alters the compaction of the chromatin fiber, increases the cellular sensitivity to ionizing radiation and the tumor burden of mice. The mortality and tumor burden of ionizing radiation-treated Hmgn1-/- mice is higher than that of their Hmgn1+/+ littermates. Hmgn1-/- fibroblasts have an altered G2-M checkpoint activation and are hypersensitive to ionizing radiation. The ionizing radiation hypersensitivity and the aberrant G2-M checkpoint activation of Hmgn1-/- fibroblasts can be reverted by transfections with plasmids expressing wild-type HMGN1, but not with plasmids expressing mutant HMGN proteins that do not bind to chromatin. Transformed Hmgn1-/- fibroblasts grow in soft agar and produce tumors in nude mice with a significantly higher efficiency than Hmgn1+/+ fibroblasts, suggesting that loss of HMGN1 protein disrupts cellular events controlling proliferation and growth. Hmgn1-/- mice have a higher incidence of multiple malignant tumors and metastases than their Hmgn1+/+ littermates. We suggest that HMGN1 optimizes the cellular response to ionizing radiation and to other tumorigenic events; therefore, loss of this protein increases the tumor burden in mice. JF - Cancer research AU - Birger, Yehudit AU - Catez, Frédéric AU - Furusawa, Takashi AU - Lim, Jae-Hwan AU - Prymakowska-Bosak, Marta AU - West, Katherine L AU - Postnikov, Yuri V AU - Haines, Diana C AU - Bustin, Michael AD - Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 6711 EP - 6718 VL - 65 IS - 15 SN - 0008-5472, 0008-5472 KW - HMGN1 Protein KW - 0 KW - Index Medicus KW - Animals KW - G2 Phase -- radiation effects KW - Mice, Nude KW - Mice KW - Fibroblasts -- radiation effects KW - Fibroblasts -- cytology KW - Male KW - Female KW - Cell Division -- radiation effects KW - HMGN1 Protein -- physiology KW - HMGN1 Protein -- metabolism KW - Neoplasms, Radiation-Induced -- pathology KW - Cell Transformation, Neoplastic -- radiation effects KW - Cell Transformation, Neoplastic -- metabolism KW - Neoplasms, Radiation-Induced -- metabolism KW - Radiation Tolerance -- physiology KW - HMGN1 Protein -- deficiency UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68435942?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Increased+tumorigenicity+and+sensitivity+to+ionizing+radiation+upon+loss+of+chromosomal+protein+HMGN1.&rft.au=Birger%2C+Yehudit%3BCatez%2C+Fr%C3%A9d%C3%A9ric%3BFurusawa%2C+Takashi%3BLim%2C+Jae-Hwan%3BPrymakowska-Bosak%2C+Marta%3BWest%2C+Katherine+L%3BPostnikov%2C+Yuri+V%3BHaines%2C+Diana+C%3BBustin%2C+Michael&rft.aulast=Birger&rft.aufirst=Yehudit&rft.date=2005-08-01&rft.volume=65&rft.issue=15&rft.spage=6711&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-04 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: EMBO J. 1999 Dec 1;18(23):6585-98 [10581233] Nature. 2000 Apr 6;404(6778):604-9 [10766243] Nature. 2000 Nov 23;408(6811):433-9 [11100718] Mol Cell Biol. 2001 Aug;21(15):5169-78 [11438671] Trends Biochem Sci. 2001 Jul;26(7):431-7 [11440855] Mol Cell. 2001 Jul;8(1):57-69 [11511360] Curr Opin Oncol. 2001 Nov;13(6):477-83 [11673688] Curr Opin Genet Dev. 2002 Feb;12(1):73-9 [11790558] EMBO Rep. 2002 Jan;3(1):28-33 [11799057] Curr Opin Genet Dev. 2002 Apr;12(2):162-9 [11893489] Science. 2002 May 3;296(5569):922-7 [11934988] EMBO Rep. 2002 Aug;3(8):760-6 [12151335] Oncogene. 2002 Sep 9;21(40):6228-33 [12214253] Nat Cell Biol. 2002 Dec;4(12):993-7 [12447390] Nature. 2003 Jan 30;421(6922):499-506 [12556884] Nat Rev Cancer. 2003 Mar;3(3):155-68 [12612651] EMBO J. 2003 Apr 1;22(7):1665-75 [12660172] Nat Cell Biol. 2003 Jul;5(7):675-9 [12792649] Cell. 2003 Aug 8;114(3):359-70 [12914700] Cell. 2003 Aug 8;114(3):371-83 [12914701] Nat Cell Biol. 2004 Feb;6(2):168-70 [14755273] Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1427-8 [14757822] Genes Dev. 2004 Mar 15;18(6):602-16 [15075289] J Exp Med. 2004 Jun 21;199(12):1671-7 [15197225] Mol Cell. 2004 Aug 27;15(4):573-84 [15327773] Science. 1994 Aug 5;265(5173):796-9 [8047885] EMBO J. 1995 Apr 3;14(7):1478-89 [7729423] Genes Dev. 1995 Aug 15;9(16):1978-91 [7649479] Cell. 1997 Nov 28;91(5):649-59 [9393858] J Biol Chem. 1998 Apr 17;273(16):9409-14 [9545265] Prog Nucleic Acid Res Mol Biol. 1999;62:227-55 [9932456] Mol Cell Biol. 1999 Aug;19(8):5237-46 [10409715] J Cell Biol. 1999 Sep 6;146(5):905-16 [10477747] Nature. 2004 Nov 18;432(7015):316-23 [15549093] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Medication development for addictive disorders: the state of the science. AN - 68094340; 16055764 AB - In 1989, the National Institute on Drug Abuse (NIDA) established its Medications Development Program. This program has concentrated on developing pharmacotherapies for opiate and cocaine dependence and, more recently, for methamphetamine and cannabis dependence. The major goals of this program are to optimize existing treatments and to expand treatment options for physicians and patients. This review will concentrate on the development of pharmacotherapies for the following substance abuse disorders: opiate, cocaine, methamphetamine, and cannabis dependence. Left untreated, opiate and stimulant dependence are responsible for significant morbidity and mortality. For example, use of illicit opiates is associated with an increased risk of hepatitis C infection, HIV infection, and other medical consequences, e.g., an overdose. The NIDA Medications Development Program has had success in developing, with pharmaceutical partners, levomethadyl acetate, buprenorphine, and buprenorphine/naloxone for opiate dependence. Moreover, several marketed medications have shown promise in reducing cocaine use. Of interest, these medications likely operate through diverse neurochemical mechanisms, suggesting that combination therapy may be a rational next step that could increase treatment gains further in cocaine-dependent patients. The Medications Development Program has also identified multiple neuronal mechanisms that are altered by chronic administration of drugs of abuse. Advances in neuroscience have identified changes in conditioned cueing, drug priming, stress-induced increases in drug intake, and reduced frontal inhibitory mechanisms as all being possible for the development of, maintenance of, and possible relapse to, addiction. Potential medications that modulate these mechanisms are highlighted. JF - The American journal of psychiatry AU - Vocci, Frank J AU - Acri, Jane AU - Elkashef, Ahmed AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, 6001 Executive Blvd., Rm. 4133, MSC 9551, Bethesda, MD 20892-9551, USA. fv6k@nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1432 EP - 1440 VL - 162 IS - 8 SN - 0002-953X, 0002-953X KW - Naloxone KW - 36B82AMQ7N KW - Buprenorphine KW - 40D3SCR4GZ KW - Methadyl Acetate KW - L59OC40KWJ KW - Dopamine KW - VTD58H1Z2X KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Animals KW - Buprenorphine -- therapeutic use KW - Chemistry, Pharmaceutical KW - Humans KW - National Institutes of Health (U.S.) -- organization & administration KW - Cocaine-Related Disorders -- drug therapy KW - Clinical Trials as Topic KW - Dopamine -- physiology KW - Opioid-Related Disorders -- drug therapy KW - Drug Design KW - Drug Therapy, Combination KW - Drug Industry -- organization & administration KW - Naloxone -- therapeutic use KW - Methadyl Acetate -- therapeutic use KW - Program Development -- methods KW - Secondary Prevention KW - Substance-Related Disorders -- physiopathology KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68094340?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Medication+development+for+addictive+disorders%3A+the+state+of+the+science.&rft.au=Vocci%2C+Frank+J%3BAcri%2C+Jane%3BElkashef%2C+Ahmed&rft.aulast=Vocci&rft.aufirst=Frank&rft.date=2005-08-01&rft.volume=162&rft.issue=8&rft.spage=1432&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Developments in the epidemiology of drug use and drug use disorders. AN - 68092891; 16055770 AB - The past 30 years of research on the epidemiology of drug use, drug use disorders, and related conditions, such as HIV, has provided major insight into these conditions. Drug use peaked in the late 1970s, decreased across the 1980s, increased in the 1990s, and has remained stable during the past few years. Within this broad pattern, specific epidemics of crack cocaine, amphetamines, club drugs (such as Ecstasy), heroin, and prescription opioids and associated epidemics of HIV and other infectious diseases have been identified and tracked. Besides major accomplishments in surveillance, the epidemiology of drug use and drug use disorders has traditionally focused on identifying risk factors at the individual (genetic factors, high-risk behaviors), family (child abuse), neighborhood (high availability of drugs), and societal (policies and laws) levels as domains of influence, not as components of interrelated processes. Research includes careful cross-sectional and longitudinal observational studies as well as clinical epidemiological experiments in which prevention interventions test specific etiological theories. Building on this background, the next challenges for the epidemiology of drug use and drug use disorders will be to link individual vulnerabilities with specific environmental factors by using multilevel methodological approaches. For example, what are the environmental factors that interact with individual vulnerabilities to produce drug addictions and drug consequences such as HIV? Research in genetic epidemiology has demonstrated the potential for studies of interactions of genetic and environmental factors. The field needs to focus on linking science with epidemiology to make progress in understanding these complex health conditions. JF - The American journal of psychiatry AU - Compton, Wilson M AU - Thomas, Yonette F AU - Conway, Kevin P AU - Colliver, James D AD - Division of Epidemiology, Services, and Prevention Research, National Institute on Drug Abuse, 6001 Executive Blvd., MSC 9589, Bethesda, MD 20892-9589, USA. wcompton@nida.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1494 EP - 1502 VL - 162 IS - 8 SN - 0002-953X, 0002-953X KW - Abridged Index Medicus KW - Index Medicus KW - Students -- statistics & numerical data KW - Risk-Taking KW - Epidemiologic Methods KW - Humans KW - HIV Infections -- etiology KW - Child KW - Comorbidity KW - Ethnic Groups -- statistics & numerical data KW - Epidemiologic Studies KW - Risk Factors KW - Adolescent Behavior -- psychology KW - Health Surveys KW - Marijuana Abuse -- psychology KW - Marijuana Abuse -- epidemiology KW - Disease Outbreaks -- statistics & numerical data KW - Adolescent KW - United States -- epidemiology KW - HIV Infections -- epidemiology KW - Social Environment KW - Substance-Related Disorders -- psychology KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68092891?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Developments+in+the+epidemiology+of+drug+use+and+drug+use+disorders.&rft.au=Compton%2C+Wilson+M%3BThomas%2C+Yonette+F%3BConway%2C+Kevin+P%3BColliver%2C+James+D&rft.aulast=Compton&rft.aufirst=Wilson&rft.date=2005-08-01&rft.volume=162&rft.issue=8&rft.spage=1494&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Monoamine oxidase-A is a major target gene for glucocorticoids in human skeletal muscle cells. AN - 68088781; 15946989 AB - Skeletal myopathy is a common complication of endogenous and exogenous glucocorticoid excess, yet its pathogenetic mechanisms remain unclear. There is accumulating evidence that mitochondrial dysfunction and oxidative stress are involved in this process. To explore the glucocorticoid-induced transcriptional adaptations that may affect mitochondrial function in skeletal muscle, we studied gene expression profiles in dexamethasone-treated primary human skeletal myocytes using a cDNA microarray, which contains 501 mitochondria-related genes. We found that monoamine oxidase A (MAO-A) was the most significantly up-regulated gene. MAO-A is the primary enzyme metabolizing catecholamines and dietary amines, and its role in skeletal muscle remains largely unexplored. Dexamethasone induced dose- and time-dependent increases of MAO-A gene and protein expression, while its effects on MAO-B were minimal. Both the glucocorticoid receptor (GR) and the Sp1 transcription factor were required for dexamethasone-induced MAO-A mRNA expression, as blockade of the GR with RU 486 or ablation of Sp1 binding with mithramycin abrogated MAO-A mRNA induction. The observed dexamethasone effect was biologically functional, as this steroid significantly increased MAO-mediated hydrogen peroxide production. We suggest that MAO-A-mediated oxidative stress can lead to cell damage, representing a novel pathogenetic mechanism for glucocorticoid-induced myopathy and a potential target for therapeutic intervention. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Manoli, Irini AU - Le, Hanh AU - Alesci, Salvatore AU - McFann, Kimberly K AU - Su, Yan A AU - Kino, Tomoshige AU - Chrousos, George P AU - Blackman, Marc R AD - Endocrine Section, Laboratory of Clinical Investigation, NCCAM, NIH, Bethesda, Maryland 20892, USA. manolii@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1359 EP - 1361 VL - 19 IS - 10 KW - Monoamine Oxidase Inhibitors KW - 0 KW - RNA, Messenger KW - Receptors, Glucocorticoid KW - Sp1 Transcription Factor KW - Dexamethasone KW - 7S5I7G3JQL KW - Hydrogen Peroxide KW - BBX060AN9V KW - Monoamine Oxidase KW - EC 1.4.3.4 KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Sp1 Transcription Factor -- physiology KW - Dose-Response Relationship, Drug KW - Humans KW - Hydrogen Peroxide -- metabolism KW - RNA, Messenger -- analysis KW - Receptors, Glucocorticoid -- physiology KW - Transcriptional Activation KW - Monoamine Oxidase Inhibitors -- pharmacology KW - Promoter Regions, Genetic KW - Muscular Diseases -- chemically induced KW - Cells, Cultured KW - Adult KW - Adolescent KW - Muscle Fibers, Skeletal -- enzymology KW - Male KW - Muscle Fibers, Skeletal -- drug effects KW - Dexamethasone -- toxicity KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Monoamine Oxidase -- biosynthesis KW - Muscle, Skeletal -- metabolism KW - Muscle, Skeletal -- drug effects KW - Monoamine Oxidase -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68088781?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=Monoamine+oxidase-A+is+a+major+target+gene+for+glucocorticoids+in+human+skeletal+muscle+cells.&rft.au=Manoli%2C+Irini%3BLe%2C+Hanh%3BAlesci%2C+Salvatore%3BMcFann%2C+Kimberly+K%3BSu%2C+Yan+A%3BKino%2C+Tomoshige%3BChrousos%2C+George+P%3BBlackman%2C+Marc+R&rft.aulast=Manoli&rft.aufirst=Irini&rft.date=2005-08-01&rft.volume=19&rft.issue=10&rft.spage=1359&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-06 N1 - Date created - 2005-07-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Secondhand smoke exposure in early life and the risk of breast cancer among never smokers (United States). AN - 68087132; 16049807 AB - Evidence is increasing that some early life exposures affect breast cancer risk. Exposure to secondhand smoke (SHS) during childhood may be one such exposure. As part of the WEB Study (Western New York Exposures and Breast Cancer Study), we conducted a population-based, case-control study with 1166 women aged 35 to 79 diagnosed with histologically confirmed, primary, incident breast cancer. Controls (n = 2105) were randomly selected from the Department of Motor Vehicles driver's license list ( age 65). Participants were queried regarding household and workplace SHS exposure. Person-years of lifetime cumulative SHS exposure were computed as well as cumulative exposure up to 21 years of age. Unconditional logistic regression adjusting for potential confounders was used to calculate odds ratios (OR) and 95% confidence intervals (95% CI). Lifetime cumulative exposure to household SHS was not associated with an increase in breast cancer risk for premenopausal (OR = 1.17, 95% CI = 0.54-2.56) or postmenopausal (OR = 1.29; 95% CI = 0.82-2.01) women. Neither was risk increased among women exposed to SHS before the age of 21 or at the time of birth, menarche, or a women's first birth. In this study, exposure to SHS either in adult or early life does not appear to be associated with the risk of breast cancer. JF - Cancer causes & control : CCC AU - Bonner, Matthew R AU - Nie, Jing AU - Han, Daikwon AU - Vena, John E AU - Rogerson, Peter AU - Muti, Paola AU - Trevisan, Maurizio AU - Edge, Stephen B AU - Freudenheim, Jo L AD - Department of Social and Preventive Medicine, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY 14214, USA. bonnerm@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 683 EP - 689 VL - 16 IS - 6 SN - 0957-5243, 0957-5243 KW - Tobacco Smoke Pollution KW - 0 KW - Index Medicus KW - Risk Factors KW - Humans KW - Adult KW - Case-Control Studies KW - Aged KW - Middle Aged KW - New York -- epidemiology KW - Menopause KW - Female KW - Tobacco Smoke Pollution -- adverse effects KW - Environmental Exposure KW - Breast Neoplasms -- etiology KW - Breast Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68087132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+causes+%26+control+%3A+CCC&rft.atitle=Secondhand+smoke+exposure+in+early+life+and+the+risk+of+breast+cancer+among+never+smokers+%28United+States%29.&rft.au=Bonner%2C+Matthew+R%3BNie%2C+Jing%3BHan%2C+Daikwon%3BVena%2C+John+E%3BRogerson%2C+Peter%3BMuti%2C+Paola%3BTrevisan%2C+Maurizio%3BEdge%2C+Stephen+B%3BFreudenheim%2C+Jo+L&rft.aulast=Bonner&rft.aufirst=Matthew&rft.date=2005-08-01&rft.volume=16&rft.issue=6&rft.spage=683&rft.isbn=&rft.btitle=&rft.title=Cancer+causes+%26+control+%3A+CCC&rft.issn=09575243&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-20 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Idiopathic intracranial hypertension following kidney transplantation: a case report and review of the literature. AN - 68083924; 16048612 AB - A pediatric kidney transplant recipient receiving tacrolimus for immunosuppression experienced symptoms consistent with idiopathic intracranial hypertension. The diagnosis of idiopathic intracranial hypertension and possible secondary causes of intracranial hypertension are reviewed in association with the patient's clinical course. Treatment options for the reversal of intracranial hypertension are summarized. Because of the complexity of associated conditions in kidney transplant recipients, symptoms of persistent headaches, visual changes and nausea and vomiting should be promptly investigated by fundoscopic examination in the setting of immunosuppression therapy to prevent vision loss. JF - Pediatric transplantation AU - Chamberlain, Christine E AU - Fitzgibbon, Edmond AU - Wassermann, Eric M AU - Butman, John A AU - Kettl, David AU - Hale, Doug AU - Kirk, Allan D AU - Mannon, Roslyn B AD - Pharmacy Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. cchamberla@nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 545 EP - 550 VL - 9 IS - 4 SN - 1397-3142, 1397-3142 KW - Tacrolimus KW - WM0HAQ4WNM KW - Index Medicus KW - Humans KW - Magnetic Resonance Angiography KW - Overweight KW - Child KW - Female KW - Tacrolimus -- adverse effects KW - Kidney Transplantation KW - Intracranial Hypertension -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68083924?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatric+transplantation&rft.atitle=Idiopathic+intracranial+hypertension+following+kidney+transplantation%3A+a+case+report+and+review+of+the+literature.&rft.au=Chamberlain%2C+Christine+E%3BFitzgibbon%2C+Edmond%3BWassermann%2C+Eric+M%3BButman%2C+John+A%3BKettl%2C+David%3BHale%2C+Doug%3BKirk%2C+Allan+D%3BMannon%2C+Roslyn+B&rft.aulast=Chamberlain&rft.aufirst=Christine&rft.date=2005-08-01&rft.volume=9&rft.issue=4&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Pediatric+transplantation&rft.issn=13973142&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-09-12 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Safety, tolerability, and pharmacokinetics of Micafungin (FK463) in febrile neutropenic pediatric patients. AN - 68081060; 16048942 AB - Micafungin (FK463) is a new parenteral echinocandin. A multicenter, phase I, open-label, sequential-group dose escalation study was conducted to assess the safety, tolerability, and pharmacokinetics of micafungin in neutropenic pediatric patients. A total of 77 patients stratified by age (2 to 12 and 13 to 17 years) received micafungin. Therapy was initiated at 0.5 mg/kg per day and escalated to higher dose levels of 1.0, 1.5, 2.0, 3.0, and 4.0 mg/kg per day. Micafungin was administered within 24 h of initiating broad-spectrum antibacterial antibiotics for the new onset of fever and neutropenia. The most common overall adverse events in the study population were diarrhea (19.5%), epistaxis (18.2%), abdominal pain (16.9%), and headache (16.9%). Nine patients (12%) experienced adverse events considered by the investigator to be possibly related to the study drug. The most common related events were diarrhea, vomiting, and headache, all occurring in two patients each. There was no evidence of a dose-limiting toxicity as defined within the prespecified criteria of this clinical protocol. There was one death during the study due to septic shock. The pharmacokinetic profiles for micafungin over the 0.5- to 4.0-mg/kg dose range demonstrated dose linearity. Clearance, volume of distribution, and half-life remained relatively constant over the dose range and did not change with repeated administration. The overall plasma pharmacokinetic profile was similar to that observed in adults. However, there was an inverse relation between age and clearance. For patients 2 to 8 years old, clearance was approximately 1.35 times that of patients >/=9 years of age. In summary, micafungin over a dosage range between 0.5 and 4.0 mg/kg/day in 77 febrile neutropenic pediatric patients displayed linear pharmacokinetics and increased clearance as a function of decreasing age. JF - Antimicrobial agents and chemotherapy AU - Seibel, Nita L AU - Schwartz, Cindy AU - Arrieta, Antonio AU - Flynn, Patricia AU - Shad, Aziza AU - Albano, Edith AU - Keirns, James AU - Lau, Wendi M AU - Facklam, David P AU - Buell, Donald N AU - Walsh, Thomas J AD - Pediatric Oncology Branch, National Cancer Institute, Bldg. 10, Rm. 13N-240, 10 Center Drive, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 3317 EP - 3324 VL - 49 IS - 8 SN - 0066-4804, 0066-4804 KW - Antifungal Agents KW - 0 KW - Echinocandins KW - Lipopeptides KW - Lipoproteins KW - Peptides, Cyclic KW - micafungin KW - R10H71BSWG KW - Index Medicus KW - Dose-Response Relationship, Drug KW - Humans KW - Drug Resistance, Fungal KW - Treatment Outcome KW - Child KW - Adolescent KW - Male KW - Female KW - Child, Preschool KW - Lipoproteins -- pharmacokinetics KW - Fever -- etiology KW - Peptides, Cyclic -- administration & dosage KW - Lipoproteins -- administration & dosage KW - Antifungal Agents -- pharmacokinetics KW - Antifungal Agents -- adverse effects KW - Peptides, Cyclic -- adverse effects KW - Mycoses -- prevention & control KW - Neutropenia -- complications KW - Lipoproteins -- adverse effects KW - Mycoses -- drug therapy KW - Antifungal Agents -- administration & dosage KW - Peptides, Cyclic -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68081060?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+agents+and+chemotherapy&rft.atitle=Safety%2C+tolerability%2C+and+pharmacokinetics+of+Micafungin+%28FK463%29+in+febrile+neutropenic+pediatric+patients.&rft.au=Seibel%2C+Nita+L%3BSchwartz%2C+Cindy%3BArrieta%2C+Antonio%3BFlynn%2C+Patricia%3BShad%2C+Aziza%3BAlbano%2C+Edith%3BKeirns%2C+James%3BLau%2C+Wendi+M%3BFacklam%2C+David+P%3BBuell%2C+Donald+N%3BWalsh%2C+Thomas+J&rft.aulast=Seibel&rft.aufirst=Nita&rft.date=2005-08-01&rft.volume=49&rft.issue=8&rft.spage=3317&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+agents+and+chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mycoses. 1999;42(7-8):431-42 [10546484] Clin Infect Dis. 2004 Nov 15;39(10):1407-16 [15546073] Antimicrob Agents Chemother. 2000 Mar;44(3):614-8 [10681327] Antimicrob Agents Chemother. 2000 Mar;44(3):619-21 [10681328] J Antimicrob Chemother. 2000 Sep;46(3):485-7 [10980180] Bone Marrow Transplant. 2000 Nov;26(9):999-1004 [11100280] Antimicrob Agents Chemother. 2001 Dec;45(12):3322-7 [11709303] J Antibiot (Tokyo). 2002 Feb;55(2):219-22 [12003006] Antimicrob Agents Chemother. 2002 Jun;46(6):1857-69 [12019101] Cancer. 2002 Oct 15;95(8):1767-72 [12365026] Cancer. 2003 May 1;97(9):2229-35 [12712476] Lancet. 2003 Oct 4;362(9390):1142-51 [14550704] Antimicrob Agents Chemother. 2004 Jun;48(6):2166-72 [15155217] Leuk Lymphoma. 2004 Apr;45(4):669-80 [15160938] Aliment Pharmacol Ther. 2004 Aug 15;20(4):475-81 [15298643] Am J Med. 1987 Dec;83(6):1103-10 [3332568] J Clin Oncol. 1990 Feb;8(2):280-6 [2299371] J Pediatr. 1992 Jun;120(6):987-93 [1593362] Bone Marrow Transplant. 1996 Dec;18 Suppl 3:S15-20 [8971401] Med Pediatr Oncol. 1998 Apr;30(4):233-9 [9473758] Clin Infect Dis. 1999 Nov;29(5):1210-9 [10524965] Antimicrob Agents Chemother. 2000 Jan;44(1):57-62 [10602723] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Increased bioaccumulation of urethane in CYP2E1-/- versus CYP2E1+/+ mice. AN - 68080461; 15879495 AB - Urethane is a fermentation by-product and a potent animal carcinogen. Human exposure to urethane occurs through consumption of alcoholic beverages and fermented foods. Recently, CYP2E1 was identified as the primary enzyme responsible for the metabolism of [(14)C]carbonyl-labeled urethane. Subsequently, attenuation of urethane-induced cell proliferation and genotoxicity in CYP2E1-/- mice was reported. The present work compares the metabolism of single versus multiple exposures of CYP2E1-/- and CYP2E1+/+ mice to (14)C-ethyl-labeled urethane. Urethane was administered as a single 10 or 100 mg/kg gavage dose or at 100 mg/kg/day for 5 consecutive days. CYP2E1+/+ mice administered single or multiple doses exhaled 78 to 88% of dose as (14)CO(2)/day. CYP2E1-/- mice eliminated 30 to 38% of a single dose as (14)CO(2) in 24 h and plateaued after day 3 at approximately 52% of dose/day. The concentrations of urethane-derived radioactivity in plasma and tissues were dose-dependent, increased as a function of the number of doses administered, and were significantly higher in CYP2E1-/- versus CYP2E1+/+ mice. Whereas urethane was the main chemical found in the plasma and tissues of CYP2E1-/- mice, it was not detectable in CYP2E1+/+ mice. In conclusion, multiple dosing led to considerable bioaccumulation of urethane in mice of both genotypes; however, greater retention occurred in CYP2E1-/- versus CYP2E1+/+ mice. Furthermore, greater bioaccumulation of (14)C-ethyl-labeled than [(14)C]carbonyl-labeled urethane was observed in mice. Comparison of the metabolism of ethyl-versus carbonyl-labeled urethane was necessary for tracing the source of CO(2) and led us to propose for the first time that C-hydroxylation is a likely pathway of urethane metabolism. JF - Drug metabolism and disposition: the biological fate of chemicals AU - Hoffler, Undi AU - Ghanayem, Burhan I AD - Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1144 EP - 1150 VL - 33 IS - 8 SN - 0090-9556, 0090-9556 KW - Carbon Radioisotopes KW - 0 KW - Carcinogens KW - Mutagens KW - Carbon Dioxide KW - 142M471B3J KW - Urethane KW - 3IN71E75Z5 KW - Cytochrome P-450 CYP2E1 KW - EC 1.14.13.- KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Carcinogens -- toxicity KW - Mutagens -- toxicity KW - Mice KW - Tissue Distribution KW - Diet KW - Carbon Dioxide -- metabolism KW - Male KW - Mice, Knockout KW - Urethane -- pharmacokinetics KW - Cytochrome P-450 CYP2E1 -- deficiency KW - Urethane -- metabolism KW - Cytochrome P-450 CYP2E1 -- metabolism KW - Urethane -- toxicity KW - Cytochrome P-450 CYP2E1 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68080461?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.atitle=Increased+bioaccumulation+of+urethane+in+CYP2E1-%2F-+versus+CYP2E1%2B%2F%2B+mice.&rft.au=Hoffler%2C+Undi%3BGhanayem%2C+Burhan+I&rft.aulast=Hoffler&rft.aufirst=Undi&rft.date=2005-08-01&rft.volume=33&rft.issue=8&rft.spage=1144&rft.isbn=&rft.btitle=&rft.title=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-08-04 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - India: alcohol and public health. AN - 68073898; 16042631 JF - Addiction (Abingdon, England) AU - Benegal, Vivek AD - Deaddiction Centre, National Institute of Mental Health and Neurosciences, Bangalore 560029, India. vbenegal@nimhans.kar.nic.in Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1051 EP - 1056 VL - 100 IS - 8 SN - 0965-2140, 0965-2140 KW - Index Medicus KW - India -- epidemiology KW - Social Change KW - Humans KW - Public Policy KW - Sex Distribution KW - Male KW - Female KW - Prevalence KW - Culture KW - Alcohol Drinking -- trends KW - Alcohol-Related Disorders -- prevention & control KW - Alcohol Drinking -- epidemiology KW - Alcohol-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68073898?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=India%3A+alcohol+and+public+health.&rft.au=Benegal%2C+Vivek&rft.aulast=Benegal&rft.aufirst=Vivek&rft.date=2005-08-01&rft.volume=100&rft.issue=8&rft.spage=1051&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-13 N1 - Date created - 2005-07-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. AN - 68073012; 16043845 AB - Pigment epithelium-derived factor (PEDF), a protein secreted by the retinal pigment epithelium (RPE), acts on retinal survival and angiogenesis. Because hypoxia and VEGF regulate matrix metalloproteinases (MMPs), their effects on PEDF proteolysis were explored. Mouse models for retinopathy of prematurity (ROP) were used. Cultured monkey RPE cells were exposed to low oxygen and chemical hypoxia mimetics. PEDF and VEGF mRNA levels in RPE were determined by RT-PCR. MMPs were assessed by zymography, DQ-gelatin degradation solution assays, and MMP immunostaining. PEDF proteolysis was assayed in solution and followed by SDS-PAGE and immunostaining. MMP induction by VEGF was performed in baby hamster kidney (BHK) cells. Retinal R28 cell survival, ex vivo chick embryonic aortic vessel sprouting, and directed in vivo angiogenesis assays were performed. Levels of PEDF in RPE/choroid significantly decreased in the ROP model. Hypoxia decreased PEDF levels in the media conditioned by RPE cells, with no significant change in PEDF mRNA. Conversely, PEDF proteolysis, gelatinolytic activities of approximately 57-kDa and approximately 86-kDa zymogens, and MMP-2 immunoreactivities increased with hypoxia. Addition of VEGF to BHK cells caused a time and dose-related upregulation of approximately 57-kDa zymogens and of DQ-gelatinolytic and PEDF-degrading activity. The PEDF-degrading activity and approximately 57-kDa zymogens in the BHK media shared MMP protease inhibition patterns and MMP-2 immunoreactivities with those in the vitreous. Limited proteolysis with MMP-2 and -9 degraded PEDF in a Ca(+2)-dependent fashion. MMP-mediated proteolysis of PEDF abolished the retinal survival and antiangiogenic activities of the PEDF protein. Hypoxia and VEGF can downregulate PEDF through proteolytic degradation. PEDF is a novel substrate for MMP-2 and -9. These results reveal a novel posttranslational mechanism for downregulating PEDF, and provide an explanation for hypoxia-provoked increases in VEGF/PEDF ratios, in angiogenesis and/or in neuronal death. JF - Investigative ophthalmology & visual science AU - Notari, Luigi AU - Miller, Amanda AU - Martínez, Alfredo AU - Amaral, Juan AU - Ju, Meihua AU - Robinson, Gregory AU - Smith, Lois E H AU - Becerra, S Patricia AD - Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-0607, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 2736 EP - 2747 VL - 46 IS - 8 SN - 0146-0404, 0146-0404 KW - Eye Proteins KW - 0 KW - Nerve Growth Factors KW - RNA, Messenger KW - Serpins KW - Vascular Endothelial Growth Factor A KW - pigment epithelium-derived factor KW - Matrix Metalloproteinase 2 KW - EC 3.4.24.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Animals KW - Oxygen -- toxicity KW - Electrophoresis, Polyacrylamide Gel KW - Pigment Epithelium of Eye -- metabolism KW - Chick Embryo KW - Humans KW - Macaca KW - Neovascularization, Pathologic -- metabolism KW - Infant, Newborn KW - Disease Models, Animal KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - Animals, Newborn KW - Blotting, Western KW - RNA, Messenger -- metabolism KW - Down-Regulation KW - Pigment Epithelium of Eye -- drug effects KW - Cells, Cultured KW - Mice, Inbred C57BL KW - Pigment Epithelium of Eye -- cytology KW - Vascular Endothelial Growth Factor A -- genetics KW - Vascular Endothelial Growth Factor A -- metabolism KW - Cricetinae KW - Retinopathy of Prematurity -- enzymology KW - Nerve Growth Factors -- metabolism KW - Matrix Metalloproteinase 9 -- metabolism KW - Nerve Growth Factors -- genetics KW - Eye Proteins -- metabolism KW - Eye Proteins -- genetics KW - Serpins -- metabolism KW - Serpins -- genetics KW - Matrix Metalloproteinase 2 -- metabolism KW - Hypoxia -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68073012?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Investigative+ophthalmology+%26+visual+science&rft.atitle=Pigment+epithelium-derived+factor+is+a+substrate+for+matrix+metalloproteinase+type+2+and+type+9%3A+implications+for+downregulation+in+hypoxia.&rft.au=Notari%2C+Luigi%3BMiller%2C+Amanda%3BMart%C3%ADnez%2C+Alfredo%3BAmaral%2C+Juan%3BJu%2C+Meihua%3BRobinson%2C+Gregory%3BSmith%2C+Lois+E+H%3BBecerra%2C+S+Patricia&rft.aulast=Notari&rft.aufirst=Luigi&rft.date=2005-08-01&rft.volume=46&rft.issue=8&rft.spage=2736&rft.isbn=&rft.btitle=&rft.title=Investigative+ophthalmology+%26+visual+science&rft.issn=01460404&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neoadjuvant docetaxel followed by adjuvant doxorubicin and cyclophosphamide in patients with stage III breast cancer. AN - 68066208; 15905305 AB - To evaluate clinical and pathologic response to neoadjuvant docetaxel therapy in patients with stage III breast cancer. Forty-five patients were planned to receive four cycles of docetaxel 100 mg/m2 every 3 weeks, followed by surgery, four cycles of doxorubicin 60 mg/m2 and cyclophosphamide 600 mg/m2 (AC) every 3 weeks, radiation therapy (RT), and tamoxifen when indicated. After four cycles of neoadjuvant docetaxel, the clinical response rate within the breast was 59% (95% CI 42% to 73%) and overall (breast and axilla) was 49% (95% CI 38% to 72%) in the intention-to-treat (ITT) population. At the time of surgery, 10% (n=4) of patients had a pathologic complete response (pCR) in the breast, 27% (n=11) had a pCR within the axillary lymph nodes, and 7% (n=3) had a pCR in the breast and axilla (95% CI 2% to 21%). An additional 5% (n=2) had minimal residual invasive tumor (<5 mm). The 5-year overall survival rate was 80%. The percentage of patients with grade 3/4 neutropenia was similar during docetaxel (93%) and AC (86%), while a greater percentage of patients had febrile neutropenia during docetaxel treatment (27%) compared with AC treatment (7%). Neoadjuvant docetaxel followed by surgery, adjuvant AC, hormonal therapy where indicated, and RT is an active regimen for patients with stage III breast cancer. JF - Annals of oncology : official journal of the European Society for Medical Oncology AU - Gradishar, W J AU - Wedam, S B AU - Jahanzeb, M AU - Erban, J AU - Limentani, S A AU - Tsai, K-T AU - Olsen, S R AU - Swain, S M AD - Northwestern University Feinberg School of Medicine, Chicago, IL, USA. swains@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1297 EP - 1304 VL - 16 IS - 8 SN - 0923-7534, 0923-7534 KW - Taxoids KW - 0 KW - docetaxel KW - 15H5577CQD KW - Doxorubicin KW - 80168379AG KW - Cyclophosphamide KW - 8N3DW7272P KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Neoplasm Staging KW - Humans KW - Neoadjuvant Therapy KW - Aged KW - Doxorubicin -- administration & dosage KW - Neoplasm, Residual -- drug therapy KW - Taxoids -- administration & dosage KW - Survival Rate KW - Neoplasm Invasiveness -- pathology KW - Adult KW - Treatment Outcome KW - Middle Aged KW - Chemotherapy, Adjuvant KW - Female KW - Remission Induction KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- pathology KW - Breast Neoplasms -- surgery KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68066208?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Behavioral+Development&rft.atitle=A+comparative+study+of+child+temperament+and+parenting+in+Beijing%2C+China+and+the+western+United+States&rft.au=Porter%2C+Christian+L.%3BHart%2C+Craig+H.%3BYang%2C+Chongming%3BRobinson%2C+Clyde+C.%3BOlsen%2C+Susanne+Frost%3BZeng%2C+Qing%3BOlsen%2C+Joseph+A.%3BJin%2C+Shenghua&rft.aulast=Porter&rft.aufirst=Christian&rft.date=2005-11-01&rft.volume=29&rft.issue=6&rft.spage=541&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Behavioral+Development&rft.issn=01650254&rft_id=info:doi/10.1080%2F01650250500147402 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-09 N1 - Date created - 2005-07-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The efficacy and safety of pain management before and after implementation of hospital-wide pain management standards: is patient safety compromised by treatment based solely on numerical pain ratings? AN - 68063760; 16037164 AB - Inadequate analgesia in hospitalized patients prompted the Joint Commission on Accreditation of Healthcare Organizations in 2001 to introduce standards that require pain assessment and treatment. In response, many institutions implemented treatment guided by patient reports of pain intensity indexed with a numerical scale. Patient safety associated with treatment of pain guided by a numerical pain treatment algorithm (NPTA) has not been examined. We reviewed patient satisfaction with pain control and opioid-related adverse drug reactions before and after implementation of our NPTA. Patient satisfaction with pain management, measured on a 1-5 scale, significantly improved from 4.13 to 4.38 (P < 0.001) after implementation of an NPTA. The incidence of opioid over sedation adverse drug reactions per 100,000 inpatient hospital days increased from 11.0 pre-NPTA to 24.5 post-NPTA (P < 0.001). Of these patients, 94% had a documented decrease in their level of consciousness preceding the event. Although there was an improvement in patient satisfaction, we experienced a more than two-fold increase in the incidence of opioid over sedation adverse drug reactions in our hospital after the implementation of NPTA. Most adverse drug reactions were preceded by a documented decrease in the patient's level of consciousness, which emphasizes the importance of clinical assessment in managing pain. Although patient satisfaction with pain management has significantly improved since the adoption of pain management standards, adverse drug reactions have more than doubled. For the treatment of pain to be safe and effective, we must consider more than just a one-dimensional numerical assessment of pain. JF - Anesthesia and analgesia AU - Vila, Hector AU - Smith, Robert A AU - Augustyniak, Michael J AU - Nagi, Peter A AU - Soto, Roy G AU - Ross, Thomas W AU - Cantor, Alan B AU - Strickland, Jennifer M AU - Miguel, Rafael V AD - Department of Interdisciplinary Oncology, The H. Lee Moffitt Cancer Center and Research Institute, National Cancer Institute, Tampa, FL 33612-9497, USA. vilah@moffitt.usf.edu Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 474 EP - 80, table of contents VL - 101 IS - 2 SN - 0003-2999, 0003-2999 KW - Analgesics, Opioid KW - 0 KW - Hypnotics and Sedatives KW - Abridged Index Medicus KW - Index Medicus KW - Patient Satisfaction KW - Humans KW - Safety KW - Algorithms KW - Aged KW - Aged, 80 and over KW - Adult KW - Drug Overdose KW - Analgesics, Opioid -- therapeutic use KW - Middle Aged KW - Guidelines as Topic KW - Analgesics, Opioid -- adverse effects KW - Hypnotics and Sedatives -- adverse effects KW - Female KW - Male KW - Hospitals -- standards KW - Pain Management KW - Pain Measurement -- standards UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68063760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anesthesia+and+analgesia&rft.atitle=The+efficacy+and+safety+of+pain+management+before+and+after+implementation+of+hospital-wide+pain+management+standards%3A+is+patient+safety+compromised+by+treatment+based+solely+on+numerical+pain+ratings%3F&rft.au=Vila%2C+Hector%3BSmith%2C+Robert+A%3BAugustyniak%2C+Michael+J%3BNagi%2C+Peter+A%3BSoto%2C+Roy+G%3BRoss%2C+Thomas+W%3BCantor%2C+Alan+B%3BStrickland%2C+Jennifer+M%3BMiguel%2C+Rafael+V&rft.aulast=Vila&rft.aufirst=Hector&rft.date=2005-08-01&rft.volume=101&rft.issue=2&rft.spage=474&rft.isbn=&rft.btitle=&rft.title=Anesthesia+and+analgesia&rft.issn=00032999&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-17 N1 - Date created - 2005-07-22 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Anesth Analg. 2006 Apr;102(4):1288; author reply 1288-9 [16551941] Anesth Analg. 2006 May;102(5):1596; author reply 1596-7 [16632864] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selected DNA repair polymorphisms and gastric cancer in Poland. AN - 68057945; 15802298 AB - Impaired DNA repair capacity may adversely affect cancer risk, particularly in subjects exposed to DNA damaging carcinogens, as found in tobacco smoke, or among subjects deficient for protective factors, as found in fruits and vegetables. We studied tobacco use, fruit and vegetable intake, and common non-synonymous single nucleotide polymorphisms in four DNA repair genes in relation to gastric cancer risk, in a population-based, case-control study of 281 incident gastric cancer cases and 390 controls, in Warsaw, Poland. Multivariate logistic regression analysis was performed to calculate odds ratios (OR) and 95% confidence intervals (CI). Increased risks of gastric cancer were found for smokers (OR=3.1, CI=1.9-5.1 for pack-years>or=40 versus never smokers) and subjects with low fruit intake (OR=2.2, CI=1.3-3.6 for 1st versus 4th quartile); risk associated with vegetable intake was not statistically significant. Allele frequencies among the controls were consistent with those previously reported for the 5 polymorphisms studied: XRCC1-Arg399Gln, XPD-Lys751Gln, MGMT-Ile143Val, Leu84Phe, and XRCC3-Thr241Met. None of the studied polymorphisms were independently associated with gastric cancer risk. Smoking-associated risks, however, were greatest for carriers of the XRCC1-399 ArgArg genotype (Pinteraction=0.004). Risks associated with low intake of fruits or vegetables tended to be modified by selected polymorphisms in XRCC1, XPD and MGMT (Pinteraction=0.1-0.2). Risk modification was not found for the other repair polymorphisms. Selected DNA repair polymorphisms did not have independent effects on gastric cancer risk; however, they may modify smoking- and probably diet-related risks for this disease. These results need replication in larger epidemiological studies of gastric cancer. JF - Carcinogenesis AU - Huang, Wen-Yi AU - Chow, Wong-Ho AU - Rothman, Nat AU - Lissowska, Jolanta AU - Llaca, Victor AU - Yeager, Meredith AU - Zatonski, Witold AU - Hayes, Richard B AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. huangw@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1354 EP - 1359 VL - 26 IS - 8 SN - 0143-3334, 0143-3334 KW - Codon KW - 0 KW - Index Medicus KW - Vegetables KW - Polymorphism, Single Nucleotide KW - Codon -- genetics KW - Humans KW - Aged KW - Risk Assessment KW - Smoking KW - Poland KW - Adult KW - Middle Aged KW - Diet KW - Fruit KW - Female KW - Male KW - Life Style KW - DNA Repair -- genetics KW - Polymorphism, Genetic KW - DNA Damage KW - Stomach Neoplasms -- genetics KW - Stomach Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057945?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Selected+DNA+repair+polymorphisms+and+gastric+cancer+in+Poland.&rft.au=Huang%2C+Wen-Yi%3BChow%2C+Wong-Ho%3BRothman%2C+Nat%3BLissowska%2C+Jolanta%3BLlaca%2C+Victor%3BYeager%2C+Meredith%3BZatonski%2C+Witold%3BHayes%2C+Richard+B&rft.aulast=Huang&rft.aufirst=Wen-Yi&rft.date=2005-08-01&rft.volume=26&rft.issue=8&rft.spage=1354&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - B-cell recovery following rituximab-based therapy is associated with perturbations in stromal derived factor-1 and granulocyte homeostasis. AN - 68057326; 15718416 AB - The occurrence of delayed neutropenia following rituximab is poorly defined and of unknown cause. We hypothesized it may be related to perturbations of stromal derived factor-1 (SDF-1) and granulocyte homeostasis. Late-onset neutropenia (LON) was investigated in 130 patients with untreated aggressive B-cell lymphoma receiving DA-EPOCH (dose-adjusted etoposide, prednisone, Oncovin [vincristine], cyclophosphamide, and hydroxydaunorubicin) chemotherapy with or without rituximab. All patients were in remission and had no known causes for neutropenia. LON occurred in 6 (8%) of 76 patients receiving rituximab and 0 of 54 patients not receiving rituximab (P = .04). The median onset was 175 days (range, 77-204 days) after treatment with a median duration of 14 days (range, 11-16 days). In a subset of 24 patients, a significant correlation was found between rapid B-cell recovery and granulocyte decline over the 6-month recovery period (R = -0.53; P = .04). Rapid B-cell recovery directly correlated with prerecovery SDF-1 levels (R = 0.65; P = .015) and SDF-1 decline (R = -0.67; P = .013) after recovery. Our results suggest that early B-cell lymphopoiesis is important for B-cell recovery following rituximab, and that perturbation of SDF-1 during B-cell recovery retards neutrophil egress from the bone marrow. These findings illustrate the dual role of SDF-1 in human B-cell and granulocyte homeostasis. JF - Blood AU - Dunleavy, Kieron AU - Hakim, Frances AU - Kim, Hyun Kyung AU - Janik, John E AU - Grant, Nicole AU - Nakayama, Takayuki AU - White, Therese AU - Wright, George AU - Kwak, Larry AU - Gress, Ronald AU - Tosato, Giovanna AU - Wilson, Wyndham H AD - Experimental Transplantation and Immunology Branch, CCR, NCI, Bldg 10, Rm 12-N-226, Bethesda, MD, 20892-1868, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 795 EP - 802 VL - 106 IS - 3 SN - 0006-4971, 0006-4971 KW - Antibodies, Monoclonal KW - 0 KW - Antibodies, Monoclonal, Murine-Derived KW - CXCL12 protein, human KW - Chemokine CXCL12 KW - Chemokines, CXC KW - Rituximab KW - 4F4X42SYQ6 KW - Abridged Index Medicus KW - Index Medicus KW - Lymphopoiesis KW - Neutropenia -- etiology KW - Humans KW - Retrospective Studies KW - Neutropenia -- chemically induced KW - Aged KW - Drug Evaluation KW - Lymphoma, B-Cell -- complications KW - Lymphoma, B-Cell -- drug therapy KW - Kinetics KW - Regeneration KW - Adult KW - Middle Aged KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Male KW - Female KW - Chemokines, CXC -- physiology KW - Granulocytes -- physiology KW - Antibodies, Monoclonal -- adverse effects KW - Homeostasis KW - B-Lymphocytes -- physiology KW - Chemokines, CXC -- blood KW - Antibodies, Monoclonal -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057326?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=B-cell+recovery+following+rituximab-based+therapy+is+associated+with+perturbations+in+stromal+derived+factor-1+and+granulocyte+homeostasis.&rft.au=Dunleavy%2C+Kieron%3BHakim%2C+Frances%3BKim%2C+Hyun+Kyung%3BJanik%2C+John+E%3BGrant%2C+Nicole%3BNakayama%2C+Takayuki%3BWhite%2C+Therese%3BWright%2C+George%3BKwak%2C+Larry%3BGress%2C+Ronald%3BTosato%2C+Giovanna%3BWilson%2C+Wyndham+H&rft.aulast=Dunleavy&rft.aufirst=Kieron&rft.date=2005-08-01&rft.volume=106&rft.issue=3&rft.spage=795&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-21 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Clin Oncol. 1999 Jun;17(6):1851-7 [10561225] Immunity. 1999 Apr;10(4):463-71 [10229189] Cancer Res. 2001 Jul 1;61(13):5028-37 [11431337] Immunity. 2001 Aug;15(2):323-34 [11520466] N Engl J Med. 2002 Jan 24;346(4):235-42 [11807147] Blood. 2002 Apr 15;99(8):2685-93 [11929754] Blood. 2002 Apr 15;99(8):2703-11 [11929756] Nat Immunol. 2002 Jul;3(7):687-94 [12068293] Exp Hematol. 2002 Sep;30(9):973-81 [12225788] J Clin Invest. 2003 Jan;111(2):187-96 [12531874] Blood. 2003 Jun 15;101(12):4653-9 [12609827] Br J Haematol. 2003 Jun;121(6):913-8 [12786803] N Engl J Med. 2003 Jun 26;348(26):2691-4; discussion 2691-4 [12826650] Immunity. 2003 Oct;19(4):583-93 [14563322] J Leukoc Biol. 2003 Nov;74(5):880-8 [12960279] Bone Marrow Transplant. 2004 May;33(9):921-3 [15034544] Immunity. 2004 Jun;20(6):707-18 [15189736] Blood. 2004 Jul 15;104(2):565-71 [15054039] Nat Med. 2004 Aug;10(8):858-64 [15235597] J Immunol. 1980 Oct;125(4):1678-85 [6157744] J Exp Med. 1981 Sep 1;154(3):737-49 [7024458] Eur J Immunol. 1986 Aug;16(8):881-7 [3091375] Blood. 1994 Jan 15;83(2):435-45 [7506951] Immunol Today. 1994 Sep;15(9):450-4 [7524522] J Clin Oncol. 1997 Oct;15(10):3266-74 [9336364] Blood. 1997 Nov 1;90(9):3789-98 [9345067] J Immunol. 1998 Feb 1;160(3):1522-31 [9570576] Nature. 1998 Jun 11;393(6685):595-9 [9634238] J Clin Oncol. 2000 Sep;18(17):3135-43 [10963642] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - MnSOD inhibits proline oxidase-induced apoptosis in colorectal cancer cells. AN - 68056785; 15817612 AB - Proline oxidase (POX), localized on inner mitochondrial membranes, is encoded by a p53-induced gene and metabolically participates in p53-induced apoptosis. Previously, we showed that POX catalyzed the generation of reactive oxygen species (ROS). We and others have demonstrated that overexpression of POX, independent of p53, causes apoptotic cell death in a variety of cancer cells. But a necessary role for ROS remains uncertain. Therefore, we asked whether superoxide dismutases (SOD) and catalase (CAT), important antioxidant enzymes, might interfere with the POX-dependent induction of apoptosis. In this study, we used DLD-1 colorectal cancer cells stably transfected with the POX gene under the control of a tetracycline-inducible promoter. When doxycycline was removed from the culture medium and the expression of POX was induced, apoptotic cell death was initiated. To examine the importance of the ROS-dependent component of the pathway, we infected DLD-1 POX cells with recombinant adenoviruses containing MnSOD, CuZnSOD, CAT or varying combinations of these adenoviruses followed by induced expression of POX. The expression of MnSOD inhibited POX-induced apoptosis, but others did not. Mechanistically, mitochondria-localized MnSOD dramatically reduced the release of cytochrome c to cytosol by POX. Compared with control cells, MnSOD-expressing DLD-1 POX cells generated a higher concentration of H2O2 owing to dismutation of superoxide radicals, which was elevated by POX. Thus, these data further suggest that the generation of superoxide radicals plays a crucial role in POX-induced apoptosis and the process is partially blocked by MnSOD. JF - Carcinogenesis AU - Liu, Yongmin AU - Borchert, Gregory L AU - Donald, Steven P AU - Surazynski, Arkadiusz AU - Hu, Chien-An AU - Weydert, Christine J AU - Oberley, Larry W AU - Phang, James M AD - Metabolism and Cancer Susceptibility Section, Laboratory for Comparative Carcinogenesis, National Cancer Institute, Frederick, MD 21702, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1335 EP - 1342 VL - 26 IS - 8 SN - 0143-3334, 0143-3334 KW - Reactive Oxygen Species KW - 0 KW - Recombinant Proteins KW - Tumor Suppressor Protein p53 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Catalase KW - EC 1.11.1.6 KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Proline Oxidase KW - EC 1.5.3.- KW - Acetylcysteine KW - WYQ7N0BPYC KW - Index Medicus KW - Catalase -- metabolism KW - Apoptosis KW - Transfection KW - Recombinant Proteins -- metabolism KW - Kinetics KW - Hydrogen Peroxide -- metabolism KW - Humans KW - Cell Line, Tumor KW - Acetylcysteine -- pharmacology KW - Cell Death -- drug effects KW - Colorectal Neoplasms KW - Proline Oxidase -- metabolism KW - Superoxide Dismutase -- metabolism KW - Tumor Suppressor Protein p53 -- genetics KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056785?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.genre=dissertations+%26+theses&rft.jtitle=&rft.atitle=&rft.au=Wei%2C+Shih-Tai&rft.aulast=Wei&rft.aufirst=Shih-Tai&rft.date=1998-02-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Perceived+parenting+patterns+and+adolescents%27+coping+styles+in+Chinese+culture&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparing measures of acute bowel toxicity in patients with prostate cancer treated with external beam radiation therapy. AN - 68056379; 16029787 AB - This study strives to compare early measures of bowel toxicity in patients with prostate cancer receiving definitive or adjuvant 3D conformal external beam radiation therapy and concurrent daily endorectal application of amifostine. Eighteen patients were enrolled in the clinical study with a median follow-up of 12 months. Prescription doses ranged from 66 Gy to 76 Gy with a daily fractionation of 2 Gy. Acute bowel toxicity was measured at baseline, at Weeks 5 and 7 of radiotherapy, and at 1 and 3 months after the completion of therapy. Measures of acute bowel toxicity included the Radiation Therapy Oncology Group (RTOG) acute radiation morbidity scoring criteria, Expanded Prostate Cancer Index Composite (EPIC) self-assessment questionnaires, and proctoscopic examinations. The mean EPIC bowel scores changed significantly through the course of therapy and follow-up (p < 0.0001), with a progressive decrease in scores at Weeks 5 and 7 of treatment, a partial recovery at 3 months, and a correlation to the gold standard RTOG grade (p = 0.004). Proctoscopic toxicity scores were low, did not vary over time, and did not correlate with either EPIC or RTOG scores. The EPIC questionnaire measurements are most sensitive to changes in acute bowel toxicity through a course of radiotherapy and correlate with RTOG acute toxicity scores. Endoscopic examination of the rectal mucosa at the end and immediate follow-up of a course of therapy does not seem to be informative or reproducible between observers in the acute setting. JF - International journal of radiation oncology, biology, physics AU - Muanza, Thierry M AU - Albert, Paul S AU - Smith, Sharon AU - Godette, Denise AU - Crouse, Nancy Sears AU - Cooley-Zgela, Theresa AU - Sciuto, Linda AU - Camphausen, Kevin AU - Coleman, C Norman AU - Ménard, Cynthia AD - Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1316 EP - 1321 VL - 62 IS - 5 SN - 0360-3016, 0360-3016 KW - Radiation-Protective Agents KW - 0 KW - Amifostine KW - M487QF2F4V KW - Index Medicus KW - Radiotherapy, Conformal KW - Proctoscopy KW - Radiotherapy Dosage KW - Humans KW - Chi-Square Distribution KW - Surveys and Questionnaires KW - Quality of Life KW - Aged KW - Middle Aged KW - Statistics, Nonparametric KW - Male KW - Amifostine -- therapeutic use KW - Radiation-Protective Agents -- therapeutic use KW - Rectum -- radiation effects KW - Radiation Injuries -- pathology KW - Prostatic Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056379?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.atitle=Comparing+measures+of+acute+bowel+toxicity+in+patients+with+prostate+cancer+treated+with+external+beam+radiation+therapy.&rft.au=Muanza%2C+Thierry+M%3BAlbert%2C+Paul+S%3BSmith%2C+Sharon%3BGodette%2C+Denise%3BCrouse%2C+Nancy+Sears%3BCooley-Zgela%2C+Theresa%3BSciuto%2C+Linda%3BCamphausen%2C+Kevin%3BColeman%2C+C+Norman%3BM%C3%A9nard%2C+Cynthia&rft.aulast=Muanza&rft.aufirst=Thierry&rft.date=2005-08-01&rft.volume=62&rft.issue=5&rft.spage=1316&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.issn=03603016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selective depletion of alloreactive donor lymphocytes: a novel method to reduce the severity of graft-versus-host disease in older patients undergoing matched sibling donor stem cell transplantation. AN - 68056332; 15817673 AB - We have selectively depleted host-reactive donor T cells from peripheral blood stem cell (PBSC) transplant allografts ex vivo using an anti-CD25 immunotoxin. We report a clinical trial to decrease graft-versus-host disease (GVHD) in elderly patients receiving selectively depleted PBSC transplants from HLA-identical sibling donors. Sixteen patients (median age, 65 years [range, 51-73 years]), with advanced hematologic malignancies underwent transplantation following reduced-intensity conditioning with fludarabine and either cyclophosphamide (n = 5), melphalan (n = 5), or busulfan (n = 6). Cyclosporine was used as sole GVHD prophylaxis. The allograft contained a median of 4.5 x 10(6) CD34 cells/kg (range, 3.4-7.3 x 10(6) CD34 cells/kg) and 1.0 x 10(8)/kg (range, 0.2-1.5 x 10(8)/kg) selectively depleted T cells. Fifteen patients achieved sustained engraftment. The helper T-lymphocyte precursor (HTLp) frequency assay demonstrated successful (mean, 5-fold) depletion of host-reactive donor T cells, with conservation of third-party response in 9 of 11 cases tested. Actuarial rates of acute GVHD were 46% +/- 13% for grades II to IV and 12% +/- 8% for grades III to IV. These results suggest that allodepletion of donor cells ex vivo is clinically feasible in older patients and may reduce the rate of severe acute GVHD. Further studies with selectively depleted transplants to evaluate graft-versus-leukemia (GVL) and survival are warranted. JF - Blood AU - Solomon, Scott R AU - Mielke, Stephan AU - Savani, Bipin N AU - Montero, Aldemar AU - Wisch, Laura AU - Childs, Richard AU - Hensel, Nancy AU - Schindler, John AU - Ghetie, Victor AU - Leitman, Susan F AU - Mai, Thao AU - Carter, Charles S AU - Kurlander, Roger AU - Read, Elizabeth J AU - Vitetta, Ellen S AU - Barrett, A John AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, NHLBI, NIH Bldg 10, Hatfield CRC, Rm 3-5320, 10 Center Dr, MSC 1202, Bethesda, MD 20892-1202, USA. Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 1123 EP - 1129 VL - 106 IS - 3 SN - 0006-4971, 0006-4971 KW - Abridged Index Medicus KW - Index Medicus KW - Hematologic Neoplasms -- therapy KW - Hematologic Neoplasms -- complications KW - Humans KW - Histocompatibility KW - Graft Survival KW - Transplantation Chimera KW - Incidence KW - Aged KW - Siblings KW - Middle Aged KW - Transplantation, Homologous KW - Recurrence KW - Male KW - Female KW - Lymphocyte Depletion -- methods KW - Peripheral Blood Stem Cell Transplantation -- adverse effects KW - Peripheral Blood Stem Cell Transplantation -- methods KW - Graft vs Host Disease -- prevention & control KW - Cell Separation -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056332?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Selective+depletion+of+alloreactive+donor+lymphocytes%3A+a+novel+method+to+reduce+the+severity+of+graft-versus-host+disease+in+older+patients+undergoing+matched+sibling+donor+stem+cell+transplantation.&rft.au=Solomon%2C+Scott+R%3BMielke%2C+Stephan%3BSavani%2C+Bipin+N%3BMontero%2C+Aldemar%3BWisch%2C+Laura%3BChilds%2C+Richard%3BHensel%2C+Nancy%3BSchindler%2C+John%3BGhetie%2C+Victor%3BLeitman%2C+Susan+F%3BMai%2C+Thao%3BCarter%2C+Charles+S%3BKurlander%2C+Roger%3BRead%2C+Elizabeth+J%3BVitetta%2C+Ellen+S%3BBarrett%2C+A+John&rft.aulast=Solomon&rft.aufirst=Scott&rft.date=2005-08-01&rft.volume=106&rft.issue=3&rft.spage=1123&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-21 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Blood. 1990 Feb 1;75(3):555-62 [2297567] Biol Blood Marrow Transplant. 2004 Apr;10(4):259-68 [15077224] Blood. 1994 Jan 1;83(1):288-98 [8274744] Bone Marrow Transplant. 1994 Oct;14(4):517-24 [7858526] Blood. 1995 Aug 15;86(4):1261-8 [7632930] Blood. 1995 Sep 1;86(5):2041-50 [7655033] Bone Marrow Transplant. 1996 May;17(5):793-9 [8733700] Bone Marrow Transplant. 1996 Aug;18(2):415-20 [8864455] Transplantation. 1997 Sep 27;64(6):836-41 [9326407] Transplantation. 1997 Oct 27;64(8):1147-52 [9355832] Br J Haematol. 1998 Jun;101(3):565-70 [9633903] Transplantation. 1999 Jan 15;67(1):124-30 [9921808] Bone Marrow Transplant. 1999 Jan;23(2):137-44 [10197798] Br J Haematol. 1999 Apr;105(1):288-94 [10233396] Blood. 1999 May 15;93(10):3550-7 [10233908] Bone Marrow Transplant. 1999 May;23(10):1071-9 [10373075] Br J Haematol. 1999 Oct;107(1):169-75 [10520038] Blood. 2004 Dec 1;104(12):3429-36 [15284108] Cytotherapy. 2005;7(2):109-15 [16040390] Br J Haematol. 2000 Jun;109(3):644-51 [10886218] Leukemia. 2001 Feb;15(2):293-302 [11236950] Bone Marrow Transplant. 2001 May;27(9):949-58 [11436105] Immunol Rev. 2001 Aug;182:58-67 [11722623] Blood. 2002 Apr 15;99(8):3041-9 [11929798] Blood. 2002 May 1;99(9):3083-8 [11964269] Blood. 2002 Jul 15;100(2):375-82 [12091325] Lancet. 2002 Jul 13;360(9327):130-7 [12126823] J Exp Med. 2002 Aug 5;196(3):401-6 [12163568] Biol Blood Marrow Transplant. 2002;8(10):525-35 [12434947] Cytotherapy. 2002;4(5):395-406 [12473206] Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1180-4 [12531922] Blood. 2004 Feb 1;103(3):1158-65 [14525783] Bone Marrow Transplant. 2004 Feb;33(4):367-75 [14716351] Transplantation. 1990 Jul;50(1):1-7 [2142343] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. AN - 68054611; 16024799 AB - ING2 is a candidate tumor suppressor gene that can activate p53 by enhancing its acetylation. Here, we demonstrate that ING2 is also involved in p53-mediated replicative senescence. ING2 protein expression increased in late-passage human primary cells, and it colocalizes with serine 15-phosphorylated p53. ING2 and p53 also complexed with the histone acetyltransferase p300. ING2 enhanced the interaction between p53 and p300 and acted as a cofactor for p300-mediated p53 acetylation. The level of ING2 expression directly modulated the onset of replicative senescence. While overexpression of ING2 induced senescence in young fibroblasts in a p53-dependent manner, expression of ING2 small interfering RNA delayed the onset of senescence. Hence, ING2 can act as a cofactor of p300 for p53 acetylation and thereby plays a positive regulatory role during p53-mediated replicative senescence. JF - Molecular and cellular biology AU - Pedeux, Remy AU - Sengupta, Sagar AU - Shen, Jiang Cheng AU - Demidov, Oleg N AU - Saito, Shin'ichi AU - Onogi, Hitoshi AU - Kumamoto, Kensuke AU - Wincovitch, Stephen AU - Garfield, Susan H AU - McMenamin, Mary AU - Nagashima, Makoto AU - Grossman, Steven R AU - Appella, Ettore AU - Harris, Curtis C AD - Laboratory of Human Carcinogenesis, CCR, NCI, NIH, 37 Convent Dr., Bldg 37, Room 3068, Bethesda, MD 20892-4255, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 6639 EP - 6648 VL - 25 IS - 15 SN - 0270-7306, 0270-7306 KW - Homeodomain Proteins KW - 0 KW - ING2 protein, human KW - Nuclear Proteins KW - Receptors, Cytoplasmic and Nuclear KW - Trans-Activators KW - Tumor Suppressor Protein p53 KW - Tumor Suppressor Proteins KW - Serine KW - 452VLY9402 KW - Index Medicus KW - Acetylation KW - Phosphorylation KW - Humans KW - Protein Processing, Post-Translational KW - Cell Division -- physiology KW - Cell Proliferation KW - Serine -- metabolism KW - Cell Line KW - Tumor Suppressor Proteins -- biosynthesis KW - Receptors, Cytoplasmic and Nuclear -- physiology KW - Trans-Activators -- metabolism KW - Homeodomain Proteins -- biosynthesis KW - Tumor Suppressor Proteins -- physiology KW - Homeodomain Proteins -- physiology KW - Cell Aging -- physiology KW - Trans-Activators -- physiology KW - Receptors, Cytoplasmic and Nuclear -- biosynthesis KW - Nuclear Proteins -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism KW - Nuclear Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68054611?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biology&rft.atitle=ING2+regulates+the+onset+of+replicative+senescence+by+induction+of+p300-dependent+p53+acetylation.&rft.au=Pedeux%2C+Remy%3BSengupta%2C+Sagar%3BShen%2C+Jiang+Cheng%3BDemidov%2C+Oleg+N%3BSaito%2C+Shin%27ichi%3BOnogi%2C+Hitoshi%3BKumamoto%2C+Kensuke%3BWincovitch%2C+Stephen%3BGarfield%2C+Susan+H%3BMcMenamin%2C+Mary%3BNagashima%2C+Makoto%3BGrossman%2C+Steven+R%3BAppella%2C+Ettore%3BHarris%2C+Curtis+C&rft.aulast=Pedeux&rft.aufirst=Remy&rft.date=2005-08-01&rft.volume=25&rft.issue=15&rft.spage=6639&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biology&rft.issn=02707306&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Cancer. 2004 Apr 10;109(3):476-9 [14961591] Exp Cell Res. 1965 Mar;37:614-36 [14315085] Nature. 2004 Mar 18;428(6980):328-32 [15029197] Oncogene. 2004 Apr 12;23(16):2919-33 [15077154] Proc Natl Acad Sci U S A. 2004 May 11;101(19):7386-91 [15123817] Oncogene. 2004 May 13;23(22):4007-13 [15007388] Mol Cell. 2004 May 21;14(4):501-13 [15149599] Cancer Res. 2004 Jun 1;64(11):3748-52 [15172978] Mol Cell. 2004 Jul 2;15(1):83-94 [15225550] EMBO J. 2004 Jul 7;23(13):2554-63 [15192702] Cell Cycle. 2004 Apr;3(4):436-8 [14976431] Nature. 1990 May 31;345(6274):458-60 [2342578] Trends Biochem Sci. 1995 Feb;20(2):56-9 [7701562] Cancer Res. 1995 Jun 1;55(11):2404-9 [7538902] Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8348-52 [7667293] Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9363-7 [7568133] J Virol. 1996 Aug;70(8):5701-5 [8764092] Oncogene. 1996 Nov 21;13(10):2097-104 [8950976] Cell. 1997 Mar 7;88(5):593-602 [9054499] Mol Cell Biol. 1997 Apr;17(4):2014-9 [9121449] Cell. 1997 Aug 22;90(4):595-606 [9288740] EMBO J. 1997 Oct 1;16(19):6018-33 [9312059] Nature. 1998 Jan 15;391(6664):295-8 [9440695] EMBO J. 1999 Dec 15;18(24):7002-10 [10601022] Mol Cell Biol. 2000 Apr;20(8):2803-8 [10733583] Nature. 2000 Jul 13;406(6792):207-10 [10910364] Genes Dev. 2000 Aug 15;14(16):2015-27 [10950866] Eur J Biochem. 2001 May;268(10):2764-72 [11358490] Eur J Biochem. 2001 May;268(10):2773-8 [11358491] Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9671-6 [11481424] Mol Cell Biol. 2001 Nov;21(22):7629-40 [11604499] Cell. 2001 Dec 28;107(7):815-8 [11779456] Science. 1998 Jan 16;279(5349):349-52 [9454332] Mol Cell Biol. 1998 Mar;18(3):1611-21 [9488478] J Biol Chem. 1998 Dec 4;273(49):33048-53 [9830059] Mol Cell Biol. 1999 Apr;19(4):3103-14 [10082577] Science. 2002 Apr 19;296(5567):550-3 [11910072] J Biol Chem. 2002 Aug 16;277(33):29832-9 [12015309] Trends Cell Biol. 2002 Nov;12(11):532-8 [12446115] Oncogene. 2003 Jan 23;22(3):343-50 [12545155] EMBO J. 2003 Mar 3;22(5):1210-22 [12606585] Curr Opin Cell Biol. 2003 Apr;15(2):164-71 [12648672] Science. 2003 Apr 11;300(5617):342-4 [12690203] Cancer Res. 2003 May 15;63(10):2373-8 [12750254] Cell. 2003 Jun 13;113(6):703-16 [12809602] J Clin Pathol. 2003 Jul;56(7):491-6 [12835293] Cell. 2003 Jul 11;114(1):99-111 [12859901] EMBO J. 2003 Aug 15;22(16):4212-22 [12912919] Curr Biol. 2003 Sep 2;13(17):1549-56 [12956959] Cancer Res. 2003 Sep 15;63(18):5785-92 [14522900] Cancer Cell. 2003 Oct;4(4):311-9 [14585358] Nature. 2003 Nov 13;426(6963):194-8 [14608368] Nat Cell Biol. 2004 Feb;6(2):168-70 [14755273] Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2259-64 [14982997] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functional consequences of single nucleotide polymorphisms in the human organic anion transporter hOAT1 (SLC22A6). AN - 68046678; 15914676 AB - The human organic anion transporter hOAT1 (SLC22A6) contributes to the uptake of a range of small organic anions across the basolateral membrane of the renal proximal tubule and drives their urinary elimination. The aim of this study was to identify genetic variants of hOAT1 and to investigate potential effects on the functional properties of this transporter. Twenty single nucleotide polymorphisms (SNPs) in hOAT1 were identified in genomic DNA from 92 individuals of African, Asian, and Caucasian origin. Two SNPs encoded changes in amino acid sequence; arginine to histidine (residue 50) and lysine to isoleucine (residue 525). Significantly, these SNPs were only present in the samples of African origin. When expressed in Xenopus oocytes, wild-type R50-hOAT1 and the variants R50H-hOAT1 and K525I-hOAT1 all mediated the probenecid-sensitive uptake of the classic organic anion para-aminohippurate (PAH). Kinetic analysis indicated that the transport affinity (K(m)) for PAH was unchanged in the variants, compared with wild type. Interestingly, the K(m) for the nucleoside phosphonate analogs adefovir, cidofovir, and tenofovir seemed to be decreased in the R50H-hOAT1 variant compared with the wild type, whereas the kinetics of K525I-hOAT1 remained unchanged. In conclusion, this is the first study to identify variation of hOAT1 in a racially diverse sample and to investigate the functional properties of the resulting variants. Since hOAT1 has been suggested as the basis of nephrotoxicity induced by nucleoside phosphonate analogs, this study raises the intriguing possibility that individuals with genetic variation in hOAT1, such as R50H, may display different handling of these drugs. JF - The Journal of pharmacology and experimental therapeutics AU - Bleasby, Kelly AU - Hall, Laura A AU - Perry, Jennifer L AU - Mohrenweiser, Harvey W AU - Pritchard, John B AD - Laboratory of Pharmacology and Chemistry, The National Institute of Environmental Health Sciences, 111 T. W. Alexander Drive, Research Triangle Park, NC 27709, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 923 EP - 931 VL - 314 IS - 2 SN - 0022-3565, 0022-3565 KW - Antiviral Agents KW - 0 KW - Organic Anion Transport Protein 1 KW - Organophosphonates KW - RNA, Complementary KW - Recombinant Proteins KW - adefovir KW - 6GQP90I798 KW - Cytosine KW - 8J337D1HZY KW - DNA KW - 9007-49-2 KW - Tenofovir KW - 99YXE507IL KW - Adenine KW - JAC85A2161 KW - cidofovir KW - JIL713Q00N KW - p-Aminohippuric Acid KW - Y79XT83BJ9 KW - Index Medicus KW - Animals KW - Genetic Variation KW - Organophosphonates -- metabolism KW - Models, Molecular KW - Mutagenesis, Site-Directed -- genetics KW - Humans KW - Amino Acid Sequence KW - Reverse Transcriptase Polymerase Chain Reaction KW - Recombinant Proteins -- genetics KW - Antiviral Agents -- metabolism KW - Cytosine -- analogs & derivatives KW - RNA, Complementary -- genetics KW - Cytosine -- metabolism KW - Xenopus laevis KW - Adenine -- metabolism KW - Oocytes -- metabolism KW - Recombinant Proteins -- metabolism KW - DNA -- genetics KW - RNA, Complementary -- biosynthesis KW - Oocytes -- drug effects KW - Molecular Sequence Data KW - p-Aminohippuric Acid -- metabolism KW - Adenine -- analogs & derivatives KW - Organic Anion Transport Protein 1 -- genetics KW - Organic Anion Transport Protein 1 -- metabolism KW - Polymorphism, Single Nucleotide -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68046678?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Functional+consequences+of+single+nucleotide+polymorphisms+in+the+human+organic+anion+transporter+hOAT1+%28SLC22A6%29.&rft.au=Bleasby%2C+Kelly%3BHall%2C+Laura+A%3BPerry%2C+Jennifer+L%3BMohrenweiser%2C+Harvey+W%3BPritchard%2C+John+B&rft.aulast=Bleasby&rft.aufirst=Kelly&rft.date=2005-08-01&rft.volume=314&rft.issue=2&rft.spage=923&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparison of cannabidiol, antioxidants, and diuretics in reversing binge ethanol-induced neurotoxicity. AN - 68040315; 15878999 AB - Binge alcohol consumption in the rat induces substantial neurodegeneration in the hippocampus and entorhinal cortex. Oxidative stress and cytotoxic edema have both been shown to be involved in such neurotoxicity, whereas N-methyl-d-aspartate (NMDA) receptor activity has been implicated in alcohol withdrawal and excitoxic injury. Because the nonpsychoactive cannabinoid cannabidiol (CBD) was previously shown in vitro to prevent glutamate toxicity through its ability to reduce oxidative stress, we evaluated CBD as a neuroprotectant in a rat binge ethanol model. When administered concurrently with binge ethanol exposure, CBD protected against hippocampal and entorhinal cortical neurodegeneration in a dose-dependent manner. Similarly, the common antioxidants butylated hydroxytoluene and alpha-tocopherol also afforded significant protection. In contrast, the NMDA receptor antagonists dizocilpine (MK-801) and memantine did not prevent cell death. Of the diuretics tested, furosemide was protective, whereas the other two anion exchanger inhibitors, L-644,711 [(R)-(+)-(5,6-dichloro2,3,9,9a-tetrahydro 3-oxo-9a-propyl-1H-fluoren-7-yl)oxy acetic acid] and bumetanide, were ineffective. In vitro comparison of these diuretics indicated that furosemide is also a potent antioxidant, whereas the nonprotective diuretics are not. The lack of efficacy of L-644,711 and bumetanide suggests that the antioxidant rather than the diuretic properties of furosemide contribute most critically to its efficacy in reversing ethanol-induced neurotoxicity in vitro, in our model. This study provides the first demonstration of CBD as an in vivo neuroprotectant and shows the efficacy of lipophilic antioxidants in preventing binge ethanol-induced brain injury. JF - The Journal of pharmacology and experimental therapeutics AU - Hamelink, Carol AU - Hampson, Aidan AU - Wink, David A AU - Eiden, Lee E AU - Eskay, Robert L AD - Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulatiuon, National Institute of Mental Health/NIH, Bldg. 49, Room 5A-35, 9000 Rockville Pike, Bethesda, MD 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 780 EP - 788 VL - 314 IS - 2 SN - 0022-3565, 0022-3565 KW - Antioxidants KW - 0 KW - Central Nervous System Depressants KW - Diuretics KW - Neuroprotective Agents KW - Cannabidiol KW - 19GBJ60SN5 KW - Ethanol KW - 3K9958V90M KW - Iron KW - E1UOL152H7 KW - Index Medicus KW - Rats KW - Oxidation-Reduction KW - Animals KW - Rats, Sprague-Dawley KW - Brain Chemistry -- drug effects KW - Male KW - Catalysis KW - Ethanol -- blood KW - Central Nervous System Depressants -- toxicity KW - Central Nervous System Depressants -- antagonists & inhibitors KW - Cannabidiol -- pharmacology KW - Antioxidants -- pharmacology KW - Central Nervous System Depressants -- blood KW - Ethanol -- antagonists & inhibitors KW - Diuretics -- pharmacology KW - Ethanol -- toxicity KW - Neurotoxicity Syndromes -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68040315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Comparison+of+cannabidiol%2C+antioxidants%2C+and+diuretics+in+reversing+binge+ethanol-induced+neurotoxicity.&rft.au=Hamelink%2C+Carol%3BHampson%2C+Aidan%3BWink%2C+David+A%3BEiden%2C+Lee+E%3BEskay%2C+Robert+L&rft.aulast=Hamelink&rft.aufirst=Carol&rft.date=2005-08-01&rft.volume=314&rft.issue=2&rft.spage=780&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevalence and correlates of tobacco use and nicotine dependence among psychiatric patients in India. AN - 68038980; 16022927 AB - Tobacco use among psychiatric patients in developing countries has not been well-investigated. To address this issue, we screened consecutive admissions to a major psychiatric hospital in southern India, and assessed the prevalence and correlates of tobacco use and nicotine dependence. Patients (n=988) provided information about their use of tobacco products, and participated in an interview that included the Fagerström Test for Nicotine Dependence as well as measures of other substance use. Three hundred and fifty-one patients (36%) reported current tobacco use, with 227 (65% of all users) reporting moderate to severe nicotine dependence. Current tobacco use as well as nicotine dependence were associated with male gender, a diagnosis of bipolar disorder, and risk of other substance use problems. The cultural context of these findings, and the implications for tobacco control among psychiatric patients, are discussed. JF - Addictive behaviors AU - Chandra, Prabha S AU - Carey, Michael P AU - Carey, Kate B AU - Jairam, K R AU - Girish, N S AU - Rudresh, H P AD - Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bangalore, India. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1290 EP - 1299 VL - 30 IS - 7 SN - 0306-4603, 0306-4603 KW - Index Medicus KW - India -- epidemiology KW - Bipolar Disorder -- epidemiology KW - Hospitalization KW - Epidemiologic Methods KW - Humans KW - Adult KW - Diagnosis, Dual (Psychiatry) KW - Bipolar Disorder -- psychology KW - Aged KW - Middle Aged KW - Adolescent KW - Male KW - Female KW - Tobacco Use Disorder -- epidemiology KW - Mental Disorders -- epidemiology KW - Tobacco Use Disorder -- psychology KW - Mental Disorders -- psychology KW - Developing Countries KW - Smoking -- epidemiology KW - Tobacco, Smokeless UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68038980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addictive+behaviors&rft.atitle=Prevalence+and+correlates+of+tobacco+use+and+nicotine+dependence+among+psychiatric+patients+in+India.&rft.au=Chandra%2C+Prabha+S%3BCarey%2C+Michael+P%3BCarey%2C+Kate+B%3BJairam%2C+K+R%3BGirish%2C+N+S%3BRudresh%2C+H+P&rft.aulast=Chandra&rft.aufirst=Prabha&rft.date=2005-08-01&rft.volume=30&rft.issue=7&rft.spage=1290&rft.isbn=&rft.btitle=&rft.title=Addictive+behaviors&rft.issn=03064603&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-22 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: JAMA. 2000 Nov 22-29;284(20):2606-10 [11086367] Lancet. 1997 May 24;349(9064):1498-504 [9167458] Br J Psychiatry. 2001 Jul;179:35-8 [11435266] Compr Psychiatry. 2001 Sep-Oct;42(5):393-402 [11559866] Br J Psychiatry. 2001 Nov;179:432-7 [11689401] Pharmacol Biochem Behav. 2001 Dec;70(4):561-70 [11796154] Addict Biol. 2002 Jan;7(1):77-83 [11900626] Addict Biol. 2002 Jan;7(1):103-10 [11900629] Schizophr Res. 2002 Jul 1;56(1-2):67-74 [12084421] Psychol Addict Behav. 2003 Dec;17(4):259-65 [14640821] BMJ. 2004 Apr 3;328(7443):801-6 [15070637] Addict Behav. 1982;7(4):363-71 [7183189] JAMA. 1990 Sep 26;264(12):1546-9 [2395194] Br J Addict. 1991 Sep;86(9):1119-27 [1932883] Addiction. 1993 Jun;88(6):791-804 [8329970] Behav Genet. 1995 Mar;25(2):95-101 [7733862] BMJ. 1996 Jun 22;312(7046):1576-9 [8664667] Tob Control. 2001 Jun;10(2):181-3 [11387541] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Antiandrogen, vaccine and combination therapy in patients with nonmetastatic hormone refractory prostate cancer. AN - 68021245; 16006888 AB - There is no current standard treatment for patients with prostate cancer who have received hormonal therapy but have an increasing prostate specific antigen (PSA) without radiographic evidence of metastasis. This trial was designed to analyze toxicity, immunogenicity and time to treatment failure using vaccine, antiandrogen therapy or their sequential use. A total of 42 patients were randomized to receive vaccine vs antiandrogen therapy with nilutamide. The vaccine consisted of recombinant vaccinia viruses containing the PSA and B7.1 costimulatory genes as prime vaccinations, and avipox-PSA as boosters. After 6 months patients with an increasing PSA and no metastasis may receive a combination of both treatments. Three patients on nilutamide were removed from study secondary to grade 3 toxicities but no grade 3 toxicities were attributed to vaccine. In the vaccine arm median time to treatment failure was 9.9 months with 13 of 21 decreases in PSA velocity vs 7.6 months with 16 of 21 decreases in PSA velocity in the nilutamide arm (p =0.28). Of the patients in the nilutamide arm 8 had vaccine added at the time of PSA progression. Median time to treatment failure with combined therapy was 5.2 months, with a median duration from study entry of 15.9 months. Of the patients in the vaccine arm 12 had nilutamide added at the time of PSA progression. Median time to treatment failure with combined therapy was 13.9 months and a median of 25.9 months from initiation of therapy. Further studies are merited to investigate the role of combining vaccine with antiandrogen therapy or vaccine followed by vaccine plus antiandrogen therapy in this patient population. JF - The Journal of urology AU - Arlen, Philip M AU - Gulley, James L AU - Todd, Nushin AU - Lieberman, Ronald AU - Steinberg, Seth M AU - Morin, Steve AU - Bastian, Anne AU - Marte, Jennifer AU - Tsang, Kwong-Yok AU - Beetham, Patricia AU - Grosenbach, Douglas W AU - Schlom, Jeffrey AU - Dahut, William AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 539 EP - 546 VL - 174 IS - 2 SN - 0022-5347, 0022-5347 KW - Androgen Antagonists KW - 0 KW - Cancer Vaccines KW - Imidazolidines KW - nilutamide KW - 51G6I8B902 KW - Prostate-Specific Antigen KW - EC 3.4.21.77 KW - Abridged Index Medicus KW - Index Medicus KW - Aged, 80 and over KW - Humans KW - Prostate-Specific Antigen -- blood KW - Disease Progression KW - Aged KW - Middle Aged KW - Male KW - Prostatic Neoplasms -- mortality KW - Cancer Vaccines -- chemistry KW - Androgen Antagonists -- therapeutic use KW - Cancer Vaccines -- therapeutic use KW - Imidazolidines -- therapeutic use KW - Prostatic Neoplasms -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68021245?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+urology&rft.atitle=Antiandrogen%2C+vaccine+and+combination+therapy+in+patients+with+nonmetastatic+hormone+refractory+prostate+cancer.&rft.au=Arlen%2C+Philip+M%3BGulley%2C+James+L%3BTodd%2C+Nushin%3BLieberman%2C+Ronald%3BSteinberg%2C+Seth+M%3BMorin%2C+Steve%3BBastian%2C+Anne%3BMarte%2C+Jennifer%3BTsang%2C+Kwong-Yok%3BBeetham%2C+Patricia%3BGrosenbach%2C+Douglas+W%3BSchlom%2C+Jeffrey%3BDahut%2C+William&rft.aulast=Arlen&rft.aufirst=Philip&rft.date=2005-08-01&rft.volume=174&rft.issue=2&rft.spage=539&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+urology&rft.issn=00225347&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-16 N1 - Date created - 2005-07-11 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: J Urol. 2005 Aug;174(2):415-6 [16006855] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Handling of dioxin measurement data in the presence of non-detectable values: overview of available methods and their application in the Seveso chloracne study. AN - 67992349; 15992596 AB - Exposure measurements of concentrations that are non-detectable or near the detection limit (DL) are common in environmental research. Proper statistical treatment of non-detects is critical to avoid bias and unnecessary loss of information. In the present work, we present an overview of possible statistical strategies for handling non-detectable values, including deletion, simple substitution, distributional methods, and distribution-based imputation. Simple substitution methods (e.g., substituting 0, DL/2, DL/ radical2, or DL for the non-detects) are the most commonly applied, even though the EPA Guidance for Data Quality Assessment discouraged their use when the percentage of non-detects is >15%. Distribution-based multiple imputation methods, also known as robust or "fill-in" procedures, may produce dependable results even when 50-70% of the observations are non-detects and can be performed using commonly available statistical software. Any statistical analysis can be conducted on the imputed datasets. Results properly reflect the presence of non-detectable values and produce valid statistical inference. We describe the use of distribution-based multiple imputation in a recent investigation conducted on subjects from the Seveso population exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in which 55.6% of plasma TCDD measurements were non-detects. We suggest that distribution-based multiple imputation be the preferred method to analyze environmental data when substantial proportions of observations are non-detects. JF - Chemosphere AU - Baccarelli, Andrea AU - Pfeiffer, Ruth AU - Consonni, Dario AU - Pesatori, Angela C AU - Bonzini, Matteo AU - Patterson, Donald G AU - Bertazzi, Pier Alberto AU - Landi, Maria Teresa AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, 6120 Executive Boulevard, Bethesda, MD 20852, USA. andrea.baccarelli@unimi.it Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 898 EP - 906 VL - 60 IS - 7 SN - 0045-6535, 0045-6535 KW - Polychlorinated Dibenzodioxins KW - 0 KW - Index Medicus KW - Chemistry Techniques, Analytical -- methods KW - Humans KW - Linear Models KW - Data Interpretation, Statistical KW - Acne Vulgaris -- chemically induced KW - Italy KW - Polychlorinated Dibenzodioxins -- blood KW - Environmental Monitoring -- statistics & numerical data UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67992349?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemosphere&rft.atitle=Handling+of+dioxin+measurement+data+in+the+presence+of+non-detectable+values%3A+overview+of+available+methods+and+their+application+in+the+Seveso+chloracne+study.&rft.au=Baccarelli%2C+Andrea%3BPfeiffer%2C+Ruth%3BConsonni%2C+Dario%3BPesatori%2C+Angela+C%3BBonzini%2C+Matteo%3BPatterson%2C+Donald+G%3BBertazzi%2C+Pier+Alberto%3BLandi%2C+Maria+Teresa&rft.aulast=Baccarelli&rft.aufirst=Andrea&rft.date=2005-08-01&rft.volume=60&rft.issue=7&rft.spage=898&rft.isbn=&rft.btitle=&rft.title=Chemosphere&rft.issn=00456535&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Alarmins: chemotactic activators of immune responses. AN - 67976882; 15955682 AB - The recruitment and activation of antigen-presenting cells are critical early steps in mounting an immune response. Many microbial components and endogenous mediators participate in this process. Recent studies have identified a group of structurally diverse multifunctional host proteins that are rapidly released following pathogen challenge and/or cell death and, most importantly, are able to both recruit and activate antigen-presenting cells. These potent immunostimulants, including defensins, cathelicidin, eosinophil-derived neurotoxin, and high-mobility group box protein 1, serve as early warning signals to activate innate and adaptive immune systems. We propose to highlight these proteins' unique activities by grouping them under the novel term 'alarmins', in recognition of their role in mobilizing the immune system. JF - Current opinion in immunology AU - Oppenheim, Joost J AU - Yang, De AD - Laboratory of Molecular Immunoregulation, Center for Cancer Research, Scientific Application and International Cooperation, Inc. (SAIC)-Frederick, National Cancer Institute at Frederick, Frederick, MD 21702, USA. Oppenhei@ncifcrf.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 359 EP - 365 VL - 17 IS - 4 SN - 0952-7915, 0952-7915 KW - Anti-Bacterial Agents KW - 0 KW - Antimicrobial Cationic Peptides KW - Index Medicus KW - Animals KW - Humans KW - Antigen-Presenting Cells -- immunology KW - Infection -- microbiology KW - Anti-Bacterial Agents -- metabolism KW - Anti-Bacterial Agents -- immunology KW - Infection -- immunology KW - Antimicrobial Cationic Peptides -- immunology KW - Antimicrobial Cationic Peptides -- secretion KW - Antimicrobial Cationic Peptides -- metabolism KW - Chemotaxis -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67976882?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+opinion+in+immunology&rft.atitle=Alarmins%3A+chemotactic+activators+of+immune+responses.&rft.au=Oppenheim%2C+Joost+J%3BYang%2C+De&rft.aulast=Oppenheim&rft.aufirst=Joost&rft.date=2005-08-01&rft.volume=17&rft.issue=4&rft.spage=359&rft.isbn=&rft.btitle=&rft.title=Current+opinion+in+immunology&rft.issn=09527915&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-01 N1 - Date created - 2005-06-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cell death in HIV dementia. AN - 67971980; 15761472 AB - Many patients infected with human immunodeficiency virus type-1 (HIV-1) suffer cognitive impairment ranging from mild to severe (HIV dementia), which may result from neuronal death in the basal ganglia, cerebral cortex and hippocampus. HIV-1 does not kill neurons by infecting them. Instead, viral proteins released from infected glial cells, macrophages and/or stem cells may directly kill neurons or may increase their vulnerability to other cell death stimuli. By binding to and/or indirectly activating cell surface receptors such as CXCR4 and the N-methyl-D-aspartate receptor, the HIV-1 proteins gp120 and Tat may trigger neuronal apoptosis and excitotoxicity as a result of oxidative stress, perturbed cellular calcium homeostasis and mitochondrial alterations. Membrane lipid metabolism and inflammation may also play important roles in determining whether neurons live or die in HIV-1-infected patients. Drugs and diets that target oxidative stress, excitotoxicity, inflammation and lipid metabolism are in development for the treatment of HIV-1 patients. JF - Cell death and differentiation AU - Mattson, M P AU - Haughey, N J AU - Nath, A AD - Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. mattsonm@grc.nia.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 893 EP - 904 VL - 12 Suppl 1 SN - 1350-9047, 1350-9047 KW - Anti-Retroviral Agents KW - 0 KW - Gene Products, tat KW - HIV Envelope Protein gp120 KW - Inflammation Mediators KW - Receptors, Glutamate KW - Viral Proteins KW - tat Gene Products, Human Immunodeficiency Virus KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Virus Replication KW - Animals KW - Neurons -- metabolism KW - Apoptosis KW - Neurons -- drug effects KW - Humans KW - Viral Proteins -- pharmacology KW - Receptors, Glutamate -- metabolism KW - Neuroglia -- drug effects KW - Viral Proteins -- toxicity KW - Gene Products, tat -- pharmacology KW - Anti-Retroviral Agents -- therapeutic use KW - Neurons -- pathology KW - Inflammation Mediators -- metabolism KW - Calcium -- metabolism KW - HIV Envelope Protein gp120 -- pharmacology KW - Neuroglia -- metabolism KW - Oxidative Stress KW - Cell Death KW - Lipid Metabolism KW - HIV-1 -- genetics KW - AIDS Dementia Complex -- genetics KW - AIDS Dementia Complex -- drug therapy KW - AIDS Dementia Complex -- metabolism KW - HIV-1 -- pathogenicity KW - Brain -- pathology KW - Brain -- virology KW - Brain -- metabolism KW - HIV-1 -- physiology KW - AIDS Dementia Complex -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67971980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+death+and+differentiation&rft.atitle=Cell+death+in+HIV+dementia.&rft.au=Mattson%2C+M+P%3BHaughey%2C+N+J%3BNath%2C+A&rft.aulast=Mattson&rft.aufirst=M&rft.date=2005-08-01&rft.volume=12+Suppl+1&rft.issue=&rft.spage=893&rft.isbn=&rft.btitle=&rft.title=Cell+death+and+differentiation&rft.issn=13509047&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-23 N1 - Date created - 2005-06-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serum Erythropoietin and Aging: A Longitudinal Analysis AN - 57084113; 200600242 AB - OBJECTIVES: To determine the changes in serum erythropoietin with age in patients with and without anemia and to assess the importance of certain comorbidities on changes in erythropoietin level and the development of anemia. DESIGN: Clinical history, hematological parameters, and serum erythropoietin levels were examined at 1- to 2-year intervals for 8 to 30 years. SETTING: Baltimore Longitudinal Study on Aging (BLSA), National Institute on Aging. PARTICIPANTS: One hundred forty-three BLSA participants. MEASUREMENTS: Complete blood count and serum chemistries were performed at the time of each visit, and archived serum samples were used for erythropoietin level. RESULTS: Although all subjects were healthy and without anemia at the time of initial evaluation, some developed chronic illness - most notably hypertension and diabetes mellitus. Erythropoietin levels rose significantly for the group as a whole, and the slope of the rise was found to be greater for those who did not have associated diabetes mellitus or hypertension. During the subsequent years, subjects who developed anemia but did not have hypertension or diabetes mellitus had the greatest slope in erythropoietin rise over time, whereas those with hypertension or diabetes mellitus and anemia had the lowest erythropoietin slope. CONCLUSION: The increase in serum erythropoietin with aging may be compensation for subclinical blood loss, increased red blood cell turnover, or increased erythropoietin resistance of red cell precursors. It is suspected that, with very advanced age, or in those with compromised renal function (e.g., diabetes mellitus or hypertension), the compensatory mechanism becomes inadequate and anemia results. Illustrations, Tables, References. Adapted from the source document. JF - Journal of the American Geriatrics Society AU - Ershler, William B AU - Sheng, Shan AU - McKelvey, Julie AU - Artz, Andrew S AU - Denduluri, Neelima AU - Tecson, Josephine AU - Taub, Dennis D AU - Brant, Larry J AU - Ferrucci, Luigi AU - Longo, Dan L AD - Instit Advanced Studies Aging, Washington, DC ershlerwi@grc.nia.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 1360 EP - 1365 PB - JAGSAF VL - 53 IS - 8 SN - 0002-8614, 0002-8614 KW - erythropoietin KW - anemia KW - BLSA KW - aging KW - Longitudinal studies KW - Ageing KW - Anaemia KW - Hormones KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57084113?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Geriatrics+Society&rft.atitle=Serum+Erythropoietin+and+Aging%3A+A+Longitudinal+Analysis&rft.au=Ershler%2C+William+B%3BSheng%2C+Shan%3BMcKelvey%2C+Julie%3BArtz%2C+Andrew+S%3BDenduluri%2C+Neelima%3BTecson%2C+Josephine%3BTaub%2C+Dennis+D%3BBrant%2C+Larry+J%3BFerrucci%2C+Luigi%3BLongo%2C+Dan+L&rft.aulast=Ershler&rft.aufirst=William&rft.date=2005-08-01&rft.volume=53&rft.issue=8&rft.spage=1360&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Geriatrics+Society&rft.issn=00028614&rft_id=info:doi/10.1111%2Fj.1532-5415.2005.53416.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JAGSAF N1 - SubjectsTermNotLitGenreText - Anaemia; Ageing; Longitudinal studies; Hormones DO - http://dx.doi.org/10.1111/j.1532-5415.2005.53416.x ER - TY - JOUR T1 - Choosing healthplans all together: a deliberative exercise for allocating limited health care resources AN - 37744722; 3275667 AB - CHAT (Choosing Healthplans All Together) is an exercise in participatory decision making designed to engage the public in health care priority setting. Participants work individually and then in groups to distribute a limited number of pegs on a board as they select from a wide range of insurance options. Randomly distributed health events illustrate the consequences of insurance choices. In 1999-2000, the authors conducted fifty sessions of CHAT involving 592 residents of North Carolina. The exercise was rated highly regarding ease of use, informativeness, and enjoyment. Participants found the information believable and complete, thought the group decision-making process was fair, and were willing to abide by group decisions. CHAT holds promise as a tool to foster group deliberation, generate collective choices, and incorporate the preferences and values of consumers into allocation decisions. It can serve to inform and stimulate public dialogue about limited health care resources. Reprinted by permission of Duke University Press JF - Journal of health politics, policy and law AU - Goold, Susan Dorr AU - Biddle, Andrea K AU - Klipp, Glenn AU - Hall, Charles N AU - Danis, Marion AD - University of Michigan ; University of North Carolina ; National Institutes of Health Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 563 EP - 602 VL - 30 IS - 4 SN - 0361-6878, 0361-6878 KW - Political Science KW - U.S.A. KW - Decision making KW - Health care KW - Social participation KW - North Carolina KW - Health insurance KW - Health policy KW - Law KW - Dialogue UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37744722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+health+politics%2C+policy+and+law&rft.atitle=Choosing+healthplans+all+together%3A+a+deliberative+exercise+for+allocating+limited+health+care+resources&rft.au=Goold%2C+Susan+Dorr%3BBiddle%2C+Andrea+K%3BKlipp%2C+Glenn%3BHall%2C+Charles+N%3BDanis%2C+Marion&rft.aulast=Goold&rft.aufirst=Susan&rft.date=2005-08-01&rft.volume=30&rft.issue=4&rft.spage=563&rft.isbn=&rft.btitle=&rft.title=Journal+of+health+politics%2C+policy+and+law&rft.issn=03616878&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 5775 13521; 3322 6071 1542 11325; 11880 11878 9003; 5784 6592 4957 11923 11949 13521; 3532 2572; 7253; 5788 11888 10472; 294 433 293 14 ER - TY - JOUR T1 - Neuroeconomics AN - 37727609; 3263818 JF - Games and economic behavior AU - Glimcher, Paul W AU - Dorris, Michael C AU - Bayer, Hannah M AU - Rustichini, Aldo AU - Dickhaut, John AU - Ghirardato, Paolo AU - Smith, Kip AU - Pardo, Josè V AU - Conover, Kent L AU - Shizgal, Peter AU - Knutson, Brian AU - Peterson, Richard AU - Bechara, Antoine AU - Damasio, Antonio R AU - Houser, Daniel AU - Keane, Michael AU - McCabe, Kevin AU - Smith, Vernon AU - Leland, Jonathan W AU - Grafman, Jordan AU - Gallistel, C R AU - Bhatt, Meghana AU - Camerer, Colin F AU - Benhabib, Jess AU - Bisin, Alberto AD - New York University ; University of Minnesota ; Università di Torino ; Concordia University ; Stanford University ; University of Iowa ; George Mason University ; Yale University ; National Science Foundation ; National Institute of Neurological Disorders and Stroke ; Rutgers University ; California Institute of Technology Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 201 EP - 493 VL - 52 IS - 2 SN - 0899-8256, 0899-8256 KW - Economics KW - U.S.A. KW - Empirical tests KW - Emotions KW - Institutionalism KW - Model testing KW - Savings KW - Game theory KW - Attitude formation KW - Algorithms KW - Laboratory KW - Review articles KW - Economic psychology KW - Auctions KW - Microeconomics KW - Human behaviour KW - Economic theory KW - Experimental methods KW - Utility functions KW - Brain KW - Neuroscience KW - Vermont KW - Decision making KW - Neuropsychology KW - Economic behaviour KW - Consumption KW - Lotteries KW - Economic analysis KW - Labour supply KW - Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37727609?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+Developmental+Science&rft.atitle=Parenting%2C+culture%2C+and+context%3A+Reflections+on+excavating+culture&rft.au=Cauce%2C+Ana+Mari&rft.aulast=Cauce&rft.aufirst=Ana&rft.date=2008-10-01&rft.volume=12&rft.issue=4&rft.spage=227&rft.isbn=&rft.btitle=&rft.title=Applied+Developmental+Science&rft.issn=10888691&rft_id=info:doi/10.1080%2F10888690802388177 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - SuppNotes - Collection of 11 articles N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 5403 8010 4025; 8010 4025; 8637; 8636; 4019; 3883 971; 6071 1542 11325; 3322 6071 1542 11325; 1615 8573 11325; 1750 1678; 7548 5401 7336 3198; 4202; 3889 6071 1542 11325; 7180 12401; 13219 13221; 4196; 3985 10404; 7127 13673 4214; 1385; 6588; 10999; 918 7824; 8160 8163; 1373 1378 10404; 4631; 2805 3872 554 971; 11280 8235; 447 433 293 14 ER - TY - JOUR T1 - Experimental tests of the somatic marker hypothesis AN - 37724906; 3263368 JF - Games and economic behavior AU - Leland, Jonathan W AU - Grafman, Jordan AD - National Science Foundation ; National Institute of Neurological Disorders and Stroke Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 386 EP - 409 VL - 52 IS - 2 SN - 0899-8256, 0899-8256 KW - Economics KW - Experiments KW - Decision making KW - Neuropsychology KW - Economic theory KW - Model testing KW - Brain KW - Microeconomics KW - Neuroscience KW - Human behaviour UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/37724906?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Games+and+economic+behavior&rft.atitle=Experimental+tests+of+the+somatic+marker+hypothesis&rft.au=Leland%2C+Jonathan+W%3BGrafman%2C+Jordan&rft.aulast=Leland&rft.aufirst=Jonathan&rft.date=2005-08-01&rft.volume=52&rft.issue=2&rft.spage=386&rft.isbn=&rft.btitle=&rft.title=Games+and+economic+behavior&rft.issn=08998256&rft_id=info:doi/10.1016%2Fj.geb.2004.09.001 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 8010 4025; 4019; 6071 1542 11325; 8636; 8637; 4636 6845 6564 12622; 1750 1678; 3322 6071 1542 11325; 8160 8163 DO - http://dx.doi.org/10.1016/j.geb.2004.09.001 ER - TY - JOUR T1 - Phase II study of CCI-779 in patients with recurrent glioblastoma multiforme AN - 216498628; 16012795 AB - Purpose: Loss of PTEN, which is common in glioblastoma multiforme (GBM), results in activation of the mammalian target of rapapmycin (mTOR), thereby increasing mRNA translation of a number of key proteins required for cell-cycle progression. CCI-779 is an inhibitor of mTOR. The primary objectives of this study were to determine the efficacy of CCI-779 in patients with recurrent GBM and to further assess the toxicity of the drug. Experimental Design: CCI-779 was administered weekly at a dose of 250 mg intravenously for patients on enzyme-inducing anti-epileptic drugs (EIAEDs). Patients not on EIAEDs were initially treated at 250 mg; however, the dose was reduced to 170 mg because of intolerable side effects. Treatment was continued until unacceptable toxicity, tumor progression, or patient withdrawal. The primary endpoint was 6-month progression-free survival. Results: Forty-three patients were enrolled; 29 were not on EIAEDs. The expected toxicity profile of increased lipids, lymphopenia, and stomatitis was seen. There were no grade IV hematological toxicities and no toxic deaths. One patient was progression free at 6 months. Of the patients assessable for response, there were 2 partial responses and 20 with stabilization of disease. The median time to progression was 9 weeks. Conclusions: CCI-779 was well tolerated at this dose schedule; however, there was no evidence of efficacy in patients with recurrent GBM. Despite initial disease stabilization in approximately 50% of patients, the durability of response was short. Because of the low toxicity profile, CCI-779 may merit exploration in combination with other modalities. JF - Investigational New Drugs AU - Chang, Susan M AU - Wen, Patrick AU - Cloughesy, Timothy AU - Greenberg, Harry AU - Schiff, David AU - Conrad, Charles AU - Fink, Karen AU - Robins, H Ian AU - De Angelis, Lisa AU - Raizer, Jeffrey AU - Hess, Kenneth AU - Aldape, Ken AU - Lamborn, Kathleen R AU - Kuhn, John AU - Dancey, Janet AU - Prados, Michael D Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 357 EP - 61 CY - New York PB - Springer Science & Business Media VL - 23 IS - 4 SN - 01676997 KW - Pharmacy And Pharmacology KW - Anticonvulsants KW - Antineoplastic Agents KW - temsirolimus KW - Sirolimus KW - Disease-Free Survival KW - Drug Interactions KW - Lymphopenia -- chemically induced KW - Infusions, Intravenous KW - Humans KW - Anemia -- chemically induced KW - Aged KW - Anticonvulsants -- administration & dosage KW - Sirolimus -- therapeutic use KW - Anticonvulsants -- therapeutic use KW - Antineoplastic Agents -- adverse effects KW - Sirolimus -- adverse effects KW - Adult KW - Middle Aged KW - Hypercholesterolemia -- chemically induced KW - Male KW - Female KW - Brain Neoplasms -- drug therapy KW - Neoplasm Recurrence, Local -- drug therapy KW - Glioblastoma -- drug therapy KW - Antineoplastic Agents -- therapeutic use KW - Sirolimus -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/216498628?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aabiglobal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Investigational+New+Drugs&rft.atitle=Phase+II+study+of+CCI-779+in+patients+with+recurrent+glioblastoma+multiforme&rft.au=Chang%2C+Susan+M%3BWen%2C+Patrick%3BCloughesy%2C+Timothy%3BGreenberg%2C+Harry%3BSchiff%2C+David%3BConrad%2C+Charles%3BFink%2C+Karen%3BRobins%2C+H+Ian%3BDe+Angelis%2C+Lisa%3BRaizer%2C+Jeffrey%3BHess%2C+Kenneth%3BAldape%2C+Ken%3BLamborn%2C+Kathleen+R%3BKuhn%2C+John%3BDancey%2C+Janet%3BPrados%2C+Michael+D&rft.aulast=Chang&rft.aufirst=Susan&rft.date=2005-08-01&rft.volume=23&rft.issue=4&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Investigational+New+Drugs&rft.issn=01676997&rft_id=info:doi/10.1007%2Fs10637-005-1444-0 LA - English DB - ProQuest Central N1 - Copyright - Springer Science + Business Media, Inc. 2005 N1 - Last updated - 2014-04-30 N1 - CODEN - INNDDK DO - http://dx.doi.org/10.1007/s10637-005-1444-0 ER - TY - JOUR T1 - CpG Immunomer DNA Enhances Antisense Protein Kinase A RI alpha Inhibition of Multidrug-Resistant Colon Carcinoma Growth in Nude Mice: Molecular Basis for Combinatorial Therapy AN - 21141712; 6522362 AB - PURPOSE: CpG DNAs induce cytokines, activate natural killer cells, and elicit vigorous T-cell response leading to antitumor effects. Antisense oligodeoxynucleotides targeted against the RI alpha subunit of protein kinase A (antisense PKA RI alpha ) induce growth arrest, apoptosis, and differentiation in a variety of cancer cell lines in vitro and in vivo. This study investigated the use of a combinatorial therapy consisting of the RNA-DNA second-generation antisense PKA RI alpha and the CpG immunomer (CpG DNA linked through 3'-3' linkage containing two accessible 5' ends). Experimental Design: HCT-15 multidrug-resistant colon carcinoma growth in nude mice was used as an experimental model. The inhibitory effect on tumor growth and apoptotic activity of antisense RI alpha and CpG immunomer, singly and in combination, were measured by tumor growth, levels of RI alpha subunit, and antiapoptotic and proapoptotic proteins. Effect on host-immune system was measured by mouse spleen size, interleukin-6 (IL-6) levels in mouse blood, and nuclear factor- Kappa B (NF- Kappa B) transcription activity in mouse spleen cells. RESULTS: In combination, CpG immunomer and antisense PKA RI alpha induced additive/supra-additive effect on the inhibition of tumor growth. Antisense RI alpha but not CpG immunomer increased Bax and Bak proapoptotic protein levels and decreased Bcl-2 and RI alpha protein levels in tumor cells. CpG immunomer but not antisense RI alpha induced an enlargement of mouse spleen, increased IL-6 levels in mouse blood, and increased NF- Kappa B transcription activity in mouse spleen cells. CONCLUSIONS: These results show that type I PKA down-regulation and induction of apoptosis in tumor cells by antisense PKA RI alpha , and host-immune stimulation by CpG immunomer are responsible at the molecular level for the supra-additive effects of tumor growth inhibition. Thus, antisense PKA RI alpha and CpG immunomer in combination work cooperatively and as tumor-targeted therapeutics to treat human cancer. JF - Clinical Cancer Research AU - Nesterova, Maria V AU - Johnson, Natalie R AU - Stewart, Trina AU - Abrams, Scott AU - Cho-Chung, Yoon S AD - Authors' Affiliations: Basic Research Laboratory, Cellular Biochemistry Section and Laboratory of Tumor, Immunology and Biology, National Cancer Institute, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5950 EP - 5955 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 16 SN - 1078-0432, 1078-0432 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Interleukin 6 KW - Protein kinase A KW - Apoptosis KW - Drug resistance KW - Animal models KW - Tumor cells KW - NF- Kappa B protein KW - Antisense oligonucleotides KW - Differentiation KW - Tumor cell lines KW - Colon KW - Lymphocytes T KW - Cytokines KW - Bcl-2 protein KW - Antisense RNA KW - Natural killer cells KW - Antisense DNA KW - Spleen KW - Transcription KW - CpG islands KW - Tumors KW - Blood KW - Bax protein KW - BAK protein KW - Antitumor activity KW - N 14830:RNA KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21141712?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=CpG+Immunomer+DNA+Enhances+Antisense+Protein+Kinase+A+RI+alpha+Inhibition+of+Multidrug-Resistant+Colon+Carcinoma+Growth+in+Nude+Mice%3A+Molecular+Basis+for+Combinatorial+Therapy&rft.au=Nesterova%2C+Maria+V%3BJohnson%2C+Natalie+R%3BStewart%2C+Trina%3BAbrams%2C+Scott%3BCho-Chung%2C+Yoon+S&rft.aulast=Nesterova&rft.aufirst=Maria&rft.date=2005-08-01&rft.volume=11&rft.issue=16&rft.spage=5950&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Interleukin 6; Protein kinase A; Apoptosis; Drug resistance; Animal models; Tumor cells; NF- Kappa B protein; Differentiation; Antisense oligonucleotides; Tumor cell lines; Colon; Lymphocytes T; Cytokines; Bcl-2 protein; Antisense RNA; Natural killer cells; Transcription; Spleen; Antisense DNA; Tumors; CpG islands; Blood; Bax protein; BAK protein; Antitumor activity ER - TY - JOUR T1 - The Youden Index and the Optimal Cut-Point Corrected for Measurement Error AN - 21122445; 11157971 AB - Random measurement error can attenuate a biomarker's ability to discriminate between diseased and non-diseased populations. A global measure of biomarker effectiveness is the Youden index, the maximum difference between sensitivity, the probability of correctly classifying diseased individuals, and 1-specificity, the probability of incorrectly classifying health individuals. We present an approach for estimating the Youden index and associated optimal cut-point for a normally distributed biomarker that corrects for normally distributed random measurement error. We also provide confidence intervals for these corrected estimates using the delta method and coverage probability through simulation over a variety of situations. Applying these techniques to the biomarker thiobarbituric acid reaction substance (TBARS), a measure of sub-products of lipid peroxidation that has been proposed as a discriminating measurement for cardiovascular disease, yields a 50% increase in diagnostic effectiveness at the optimal cut-point. This result may lead to biomarkers that were once naively considered ineffective becoming useful diagnostic devices. JF - Biometrical Journal AU - Perkins, Neil J AU - Schisterman, Enrique F AD - Division of Epidemiology, Statistics and Prevention Research, National Institute of Child Health and Human Development (NICHD), National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA, schistee@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 428 EP - 441 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 47 IS - 4 SN - 0323-3847, 0323-3847 KW - Biotechnology and Bioengineering Abstracts KW - thiobarbituric acid KW - Statistics KW - Cardiovascular diseases KW - Biometrics KW - biomarkers KW - Lipid peroxidation KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21122445?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biometrical+Journal&rft.atitle=The+Youden+Index+and+the+Optimal+Cut-Point+Corrected+for+Measurement+Error&rft.au=Perkins%2C+Neil+J%3BSchisterman%2C+Enrique+F&rft.aulast=Perkins&rft.aufirst=Neil&rft.date=2005-08-01&rft.volume=47&rft.issue=4&rft.spage=428&rft.isbn=&rft.btitle=&rft.title=Biometrical+Journal&rft.issn=03233847&rft_id=info:doi/10.1002%2Fbimj.200410133 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - biomarkers; Biometrics; thiobarbituric acid; Statistics; Cardiovascular diseases; Lipid peroxidation DO - http://dx.doi.org/10.1002/bimj.200410133 ER - TY - JOUR T1 - Plasmodium falciparum: Characterization of a late asexual stage Golgi protein containing both ankyrin and DHHC domains AN - 20941878; 8250964 AB - Proteins containing the DHHC motif have been shown to function as palmitoyl transferases. The palmitoylation of proteins has been shown to play an important role in the trafficking of proteins to the proper subcellular location. Herein, we describe a protein containing both ankyrin domains and a DHHC domain that is present in the Golgi of late schizonts of P. falciparum. The timing of expression as well as the location of this protein suggests that it may play an important role in the sorting of proteins to the apical organelles during the development of the asexual stage of the parasite. JF - Experimental Parasitology AU - Seydel, K B AU - Gaur, D AU - Aravind, L AU - Subramanian, G AU - Miller, L H AD - National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA, lmiller@niaid.nih.gov Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 389 EP - 393 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl] VL - 110 IS - 4 SN - 0014-4894, 0014-4894 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources KW - Protein transport KW - Golgi apparatus KW - Parasites KW - Transferases KW - Schizonts KW - Developmental stages KW - Plasmodium falciparum KW - Endoparasites KW - palmitoylation KW - Ankyrin KW - Proteins KW - Organelles KW - Q1 08484:Species interactions: parasites and diseases KW - Q5 08501:General KW - K 03320:Cell Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20941878?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+Parasitology&rft.atitle=Plasmodium+falciparum%3A+Characterization+of+a+late+asexual+stage+Golgi+protein+containing+both+ankyrin+and+DHHC+domains&rft.au=Seydel%2C+K+B%3BGaur%2C+D%3BAravind%2C+L%3BSubramanian%2C+G%3BMiller%2C+L+H&rft.aulast=Seydel&rft.aufirst=K&rft.date=2005-08-01&rft.volume=&rft.issue=&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Boston+Globe+%28pre-1997+Fulltext%29&rft.issn=07431791&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Parasites; Transferases; Proteins; Developmental stages; Endoparasites; Golgi apparatus; Protein transport; palmitoylation; Ankyrin; Schizonts; Organelles; Plasmodium falciparum DO - http://dx.doi.org/10.1016/j.exppara.2005.03.030 ER - TY - JOUR T1 - Role of withdrawal in reinstatement of morphine-conditioned place preference AN - 20887827; 6886198 AB - Rationale: Relapse is a major characteristic of drug addiction and the primary problem in treating drug abuse. Based on the negative reinforcement view of addiction, in which the motivation to take drugs is thought to result from the desire to avoid the aversive effect of drug withdrawal, it has been theorized that withdrawal symptoms play a major role in the maintenance of and relapse to drug taking. However, the role of withdrawal in relapse has not yet been systemically investigated in the reinstatement model. Objectives: Using a conditioned place preference (CPP) paradigm, we examined the role of different morphine withdrawal states (spontaneous withdrawal, naloxone-precipitated withdrawal, and conditioned withdrawal) in relapse to drug seeking. Methods: Rats alternately received morphine (10 mg/kg, s.c.) and saline for 8 days to acquire the CPP. The morphine CPP disappeared after a 2-week extinction phase of saline-paired training. Rats were then chronically administered morphine to induce physical dependence. The different withdrawal states were induced and their roles in the reinstatement of extinguished CPP were assessed. During conditioned withdrawal, trunk blood samples were taken and the corticosterone level was measured by radioimmunoassay. To examine the role of corticotropin-releasing factor (CRF) receptor antagonist on conditioned-withdrawal-induced reinstatement of CPP, different doses of alpha -helical CRF (0.1 and 1 mu g, i.c.v.) were administered 30 min prior to the CPP testing. Results: The results show that morphine spontaneous withdrawal and naloxone-precipitated morphine withdrawal were ineffective in reinstating morphine CPP. However, the withdrawal cues significantly elicited the reinstatement of CPP and increased corticosterone level. Moreover, pretreatment with the CRF receptor antagonist alpha -helical CRF (1 mu g, i.c.v.) significantly attenuated the effects of withdrawal cues on reinstatement of CPP and corticosterone levels. Conclusion: These findings demonstrate that the cues associated with previous drug withdrawal play a major role in drug relapse and that activation of the CRF receptor is involved in conditioned-withdrawal-induced reinstatement. The present study suggests that CRF receptor antagonists might be of value in the treatment and prevention of relapse to drug seeking after long-term abstinence. JF - Psychopharmacology AU - Lu, Lin AU - Chen, Hai AU - Su, Wenjuan AU - Ge, Xin AU - Yue, Wen AU - Su, Fen AU - Ma, Lan AD - IRP/NIDA/NIH, 5500 Nathan Shock Drive, Baltimore, MD, 21224, USA, llu@intra.nida.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 90 EP - 100 PB - Springer-Verlag (Berlin), Heidelberger Platz 3 Berlin 14197 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 181 IS - 1 SN - 0033-3158, 0033-3158 KW - Animal Behavior Abstracts; Toxicology Abstracts; CSA Neurosciences Abstracts KW - Place preferences KW - Morphine KW - Extinction KW - Motivation KW - Withdrawal KW - Drug abuse KW - Reinstatement KW - Radioimmunoassay KW - Physical training KW - Corticosterone KW - Reinforcement KW - Drug addiction KW - Drugs KW - Corticotropin-releasing hormone KW - N3 11001:Behavioral and Cognitive Neuroscience KW - Y 25811:General KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20887827?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Role+of+withdrawal+in+reinstatement+of+morphine-conditioned+place+preference&rft.au=Lu%2C+Lin%3BChen%2C+Hai%3BSu%2C+Wenjuan%3BGe%2C+Xin%3BYue%2C+Wen%3BSu%2C+Fen%3BMa%2C+Lan&rft.aulast=Lu&rft.aufirst=Lin&rft.date=2005-08-01&rft.volume=181&rft.issue=1&rft.spage=90&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/10.1007%2Fs00213-005-2207-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Morphine; Place preferences; Motivation; Extinction; Withdrawal; Drug abuse; Radioimmunoassay; Reinstatement; Physical training; Corticosterone; Reinforcement; Drug addiction; Corticotropin-releasing hormone; Drugs DO - http://dx.doi.org/10.1007/s00213-005-2207-5 ER - TY - JOUR T1 - High abundance protein profiling of cystic fibrosis lung epithelial cells AN - 20859190; 6243359 AB - Protein profiles of cultured cystic fibrosis (CF) lung epithelial cells were analyzed by two-dimensional gel electrophoresis and mass spectrometry (MS). The analysis gave rise to a protein map over the pI range of 4-7, and a molecular weight range of ca. 100-10 kDa. The map contains 194 identified proteins, which were detectable by silver stain. All silver stained features were identified by matrix-assisted laser desorption/ionization-time of flight MS of tryptic peptides. Some proteins were found to be represented by multiple features on the 2-D gel. Among the high abundance proteins identified were sets of proteins associated with inflammation, including the classical NF Kappa B, p65 (RelA) and NF Kappa B, p65 (RelB). We suggest that this composite atlas of the high abundance CF lung epithelial proteome will serve as a reference database for future studies of candidate CF drugs, validating different approaches to CFTR gene therapy, and analogous investigations of other types of human lung disorders. JF - Proteomics AU - Pollard, Harvey B AU - Ji, Xiao-duo AU - Jozwik, Catherine AU - Jacobowitz, David M AD - Department of Anatomy, Physiology and Genetics, and Institute for Molecular Medicine, Uniformed Services University School of Medicine, USUHS, Bethesda, MD, USA, dwj@helix.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2210 EP - 2226 PB - Wiley-VCH, Postfach 101161 Weinheim 69451 Germany, [mailto:info@wiley-vch.de], [URL:http://www.wiley-vch.de/publish/en/] VL - 5 IS - 8 SN - 1615-9853, 1615-9853 KW - Biotechnology and Bioengineering Abstracts KW - Cystic fibrosis KW - Epithelial cells KW - Lung KW - Protein KW - Two-dimensional gel electrophoresis KW - RelB protein KW - Cystic fibrosis transmembrane conductance regulator KW - Drug development KW - Stains KW - RelA protein KW - Mass spectroscopy KW - Gel electrophoresis KW - Inflammation KW - Flight KW - Databases KW - Atlases KW - Molecular weight KW - Lasers KW - Tryptic peptides KW - proteomics KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20859190?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proteomics&rft.atitle=High+abundance+protein+profiling+of+cystic+fibrosis+lung+epithelial+cells&rft.au=Pollard%2C+Harvey+B%3BJi%2C+Xiao-duo%3BJozwik%2C+Catherine%3BJacobowitz%2C+David+M&rft.aulast=Pollard&rft.aufirst=Harvey&rft.date=2005-08-01&rft.volume=5&rft.issue=8&rft.spage=2210&rft.isbn=&rft.btitle=&rft.title=Proteomics&rft.issn=16159853&rft_id=info:doi/10.1002%2Fpmic.200401120 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-09-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - RelB protein; Epithelial cells; Cystic fibrosis transmembrane conductance regulator; Drug development; Stains; RelA protein; Gel electrophoresis; Mass spectroscopy; Inflammation; Flight; Databases; Atlases; Lung; Molecular weight; Tryptic peptides; Lasers; proteomics; Cystic fibrosis DO - http://dx.doi.org/10.1002/pmic.200401120 ER - TY - JOUR T1 - Modeling and simulations of the pharmacokinetics of fluorophore conjugated antibodies in tumor vicinity for the optimization of fluorescence-based optical imaging AN - 20490620; 8029912 AB - Background and Objectives One of the methods to detect and localize tumors in tissue is to use fluorophore conjugated specific antibodies as tumor surface markers. The goals of this study are to understand and quantify the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor. This study concludes another stage of the development of a non-invasive fluorescenated antibody-based technique for imaging and localization of tumors in vivo. Study Design/Materials and Methods A mathematical model of the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor was developed based on histological staining experiments. We present the model equations of concentrations of antibodies and free binding sites. We also present a powerful simulation tool that we developed to simulate the imaging process. We analyzed the model and studied the effects of various independent parameters on the imaging result. These parameters included initial volume of markers (injected volume), total number of binding sites, tumor size, binding and dissociation rate constants, and the diffusion coefficient. We present the relations needed between these parameters in order to optimize the imaging results. Results and Conclusions A powerful and accurate tool was developed which may assist in optimizing the imaging system results by setting the injection volume and concentration of fluorophore conjugated antibodies in tissue and approximating the time interval where maximum specific binding occurs and the tumor can be imaged. Lasers Surg. Med. JF - Lasers in Surgery and Medicine AU - Fibich, G AU - Hammer, A AU - Gannot, G AU - Gandjbakhche, A AU - Gannot, I AD - Department of Applied Mathematics, Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel, gannoti@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 155 EP - 160 PB - John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/] VL - 37 IS - 2 SN - 0196-8092, 0196-8092 KW - Biotechnology and Bioengineering Abstracts KW - Antibodies KW - Mathematical models KW - Developmental stages KW - Lasers KW - Tumors KW - fluorophores KW - Diffusion coefficient KW - imaging KW - Pharmacokinetics KW - Surface markers KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20490620?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lasers+in+Surgery+and+Medicine&rft.atitle=Modeling+and+simulations+of+the+pharmacokinetics+of+fluorophore+conjugated+antibodies+in+tumor+vicinity+for+the+optimization+of+fluorescence-based+optical+imaging&rft.au=Fibich%2C+G%3BHammer%2C+A%3BGannot%2C+G%3BGandjbakhche%2C+A%3BGannot%2C+I&rft.aulast=Fibich&rft.aufirst=G&rft.date=2005-08-01&rft.volume=37&rft.issue=2&rft.spage=155&rft.isbn=&rft.btitle=&rft.title=Lasers+in+Surgery+and+Medicine&rft.issn=01968092&rft_id=info:doi/10.1002%2Flsm.20200 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Antibodies; Mathematical models; Developmental stages; Lasers; Diffusion coefficient; fluorophores; Tumors; imaging; Surface markers; Pharmacokinetics DO - http://dx.doi.org/10.1002/lsm.20200 ER - TY - JOUR T1 - DNA Drug Design for Cancer Therapy AN - 20304165; 7653589 AB - DNA (antisense and other oligonucleotides) drug design represents a direct genetic approach for cancer treatment. Such an approach takes advantage of mechanisms that activate genes known to confer a growth advantage to neoplastic cells. The ability to block the expression of these genes allows exploration of normal growth regulation. Progress in DNA drug technology has been rapid, and the traditional antisense inhibition of gene expression is now viewed on a genomic scale. This global view has led to a new vision in antisense technology, the elimination of nonspecific and undesirable side effects, and ultimately the generation of more effective and less toxic nucleic acid medicines. Several antisense oligonucleotides are in clinical trials, are well tolerated, and are potentially active therapeutically. DNA drugs are promising molecular medicines for treating human cancer in the near future. JF - Current Pharmaceutical Design AU - Cho-Chung, Y S AD - Cellular Biochemistry Section, BRL, CCR, National Cancer Institute, Bldg. 10, Rm. 5B05, 9000 Rockville Pike, Bethesda, MD 20892-1750, USA. Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2811 EP - 2823 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 11 IS - 22 SN - 1381-6128, 1381-6128 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - antisense KW - oligonucleotides KW - dna drugs KW - gene expression KW - cancer KW - growth inhibition KW - transcription factor decoy KW - Antisense oligonucleotides KW - nucleic acids KW - Vision KW - Antisense DNA KW - Drug development KW - genomics KW - Clinical trials KW - Cancer KW - Side effects KW - N 14840:Antisense, Nucleotide Analogs KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20304165?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Pharmaceutical+Design&rft.atitle=DNA+Drug+Design+for+Cancer+Therapy&rft.au=Cho-Chung%2C+Y+S&rft.aulast=Cho-Chung&rft.aufirst=Y&rft.date=2005-08-01&rft.volume=11&rft.issue=22&rft.spage=2811&rft.isbn=&rft.btitle=&rft.title=Current+Pharmaceutical+Design&rft.issn=13816128&rft_id=info:doi/10.2174%2F1381612054546770 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Antisense oligonucleotides; nucleic acids; Vision; Antisense DNA; Drug development; genomics; Clinical trials; Side effects; Cancer DO - http://dx.doi.org/10.2174/1381612054546770 ER - TY - JOUR T1 - Beyond Single Pathway Inhibition: MEK Inhibitors as a Platform for the Development of Pharmacological Combinations with Synergistic Anti-Leukemic Effects AN - 20281482; 7653586 AB - The MEK/MAPK signaling module is a key integration point along signal transduction cascades that regulate cell growth, survival, and differentiation, and is aberrantly activated in many human tumors. In tumor cells, constitutive MAPK activation affords increased proliferation and resistance to apoptotic stimuli, including classical cytotoxic drugs. In most instances, however, MAPK inhibition has cytostatic rather than cytotoxic effects, which may explain the lack of objective responses observed in early clinical trials of MEK inhibitors. Nevertheless, amenability of the MAPK pathway to pharmacodynamic evaluation and negligible clinical toxicity make MEK inhibitors an ideal platform to build pharmacological combinations with synergistic antitumor activity. In AML, the MEK/MAPK pathway is constitutively activated in the majority of cases (75%), conferring a uniformly poor prognosis; in preclinical models of AML, MEK blockade profoundly inhibits cell growth and proliferation and downregulates the expression of several anti-apoptotic players, thereby lowering the apoptotic threshold. Apoptosis induction, however, requires concentrations of MEK inhibitors much higher than those required to inhibit proliferation. Nevertheless, MEK blockade efficiently and selectively sensitizes leukemic cells to sub-optimal doses of other apoptotic stimuli, including classical cytotoxics (nucleoside analogs, microtubule-targeted drugs, ggr-irradiation), biologicals (retinoids, interferons, arsenic trioxide), and, most interestingly, other signal transduction/apoptosis modulators (UCN-01, STI571, Bcl-2 antagonists). In most instances, these MEK inhibition-based combinations result in a striking pro-apoptotic synergism in preclinical models. Here we briefly discuss evidence suggesting that MAPK pathway inhibition could play a prominent role in the development of integrated therapeutic strategies aimed at synergistic anti-leukemic effects. JF - Current Pharmaceutical Design AU - Milella, M AU - Precupanu, C M AU - Gregorj, C AU - Ricciardi, M R AU - Petrucci, M T AU - Kornblau, S M AU - Tafuri, A AU - Andreeff, M AD - Division of Medical Oncology A, Regina Elena National Cancer Institute, Via Elio Chianesi 53, 00144 Rome, Italy Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2779 EP - 2795 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 11 IS - 21 SN - 1381-6128, 1381-6128 KW - Biotechnology and Bioengineering Abstracts KW - mapk KW - erk KW - leukemia KW - mek inhibitors KW - combination therapy KW - apoptosis KW - synergism KW - Cell survival KW - Apoptosis KW - Clinical trials KW - Tumor cells KW - Antagonists KW - Differentiation KW - Integration KW - Leukemia KW - Bcl-2 protein KW - Drugs KW - Pharmacodynamics KW - MAP kinase KW - Prognosis KW - Arsenic trioxide KW - Toxicity KW - Tumors KW - nucleoside analogs KW - Interferon KW - Cytotoxicity KW - MEK inhibitors KW - Retinoids KW - Cell proliferation KW - Antitumor activity KW - Signal transduction KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20281482?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Pharmaceutical+Design&rft.atitle=Beyond+Single+Pathway+Inhibition%3A+MEK+Inhibitors+as+a+Platform+for+the+Development+of+Pharmacological+Combinations+with+Synergistic+Anti-Leukemic+Effects&rft.au=Milella%2C+M%3BPrecupanu%2C+C+M%3BGregorj%2C+C%3BRicciardi%2C+M+R%3BPetrucci%2C+M+T%3BKornblau%2C+S+M%3BTafuri%2C+A%3BAndreeff%2C+M&rft.aulast=Milella&rft.aufirst=M&rft.date=2005-08-01&rft.volume=11&rft.issue=21&rft.spage=2779&rft.isbn=&rft.btitle=&rft.title=Current+Pharmaceutical+Design&rft.issn=13816128&rft_id=info:doi/10.2174%2F1381612054546842 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Cell survival; MAP kinase; Apoptosis; Prognosis; Arsenic trioxide; Tumors; Toxicity; Tumor cells; Clinical trials; Antagonists; nucleoside analogs; Leukemia; Integration; Differentiation; Interferon; Cytotoxicity; MEK inhibitors; Bcl-2 protein; Cell proliferation; Retinoids; Drugs; Pharmacodynamics; Signal transduction; Antitumor activity DO - http://dx.doi.org/10.2174/1381612054546842 ER - TY - JOUR T1 - New Monoclonal Antibodies to Mesothelin Useful for Immunohistochemistry, Fluorescence-Activated Cell Sorting, Western Blotting, and ELISA AN - 20237104; 6522348 AB - PURPOSE: Mesothelin is a cell surface protein that is highly expressed in some malignant tumors, and is a promising target for immunotherapy. Recent data suggests that mesothelin is an adhesive protein and may have a role in the metastases of ovarian cancer. Although a few monoclonal antibodies (MAb) to mesothelin have been produced, they have limitations for the study of expression of native mesothelin because of their low affinity or reactivity only with denatured mesothelin protein. We have produced novel MAbs to mesothelin to help study mesothelin function and to develop improved diagnosis and immunotherapy of mesothelin-expressing tumors. Experimental Design: Mesothelin-deficient mice were immunized with plasmid cDNA encoding mesothelin, and boosted with a mesothelin-rabbit IgG Fc fusion protein prior to cell fusion. Hybridomas were screened by an ELISA using plates coated with mesothelin-Fc protein. RESULTS: Seventeen hybridomas producing anti-mesothelin antibodies were established and shown to react with two epitopes on mesothelin. One group reacts with the same epitope as the low affinity antibody K1 that was originally used to identify mesothelin. The other is a new group that reacts with a new epitope. One antibody from each group was chosen for further study and shown to react strongly on ELISA, on immunohistochemistry, and by fluorescence-activated cell sorting on living cells. CONCLUSION: Our two newly established MAbs, MN and MB, have different and useful properties compared with current antibodies used for the detection of mesothelin by immunohistochemistry, fluorescence-activated cell sorting, ELISA, and Western blotting. JF - Clinical Cancer Research AU - Onda, Masanori AU - Willingham, Mark AU - Nagata, Satoshi AU - Bera, Tapan K AU - Beers, Richard AU - Ho, Mitchell AU - Hassan, Raffit AU - Kreitman, Robert J AU - Pastan, Ira AD - Authors' Affiliations: Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland and Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5840 EP - 5846 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 16 SN - 1078-0432, 1078-0432 KW - Biotechnology and Bioengineering Abstracts KW - Ovarian cancer KW - Western blotting KW - Fc KW - Cell surface KW - Enzyme-linked immunosorbent assay KW - Data processing KW - Monoclonal antibodies KW - Immunotherapy KW - Tumors KW - Plasmids KW - Cell fusion KW - Hybridoma KW - Flow cytometry KW - Metastases KW - Immunoglobulin G KW - Fusion protein KW - Adhesives KW - Manganese KW - Immunohistochemistry KW - Epitopes KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20237104?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=New+Monoclonal+Antibodies+to+Mesothelin+Useful+for+Immunohistochemistry%2C+Fluorescence-Activated+Cell+Sorting%2C+Western+Blotting%2C+and+ELISA&rft.au=Onda%2C+Masanori%3BWillingham%2C+Mark%3BNagata%2C+Satoshi%3BBera%2C+Tapan+K%3BBeers%2C+Richard%3BHo%2C+Mitchell%3BHassan%2C+Raffit%3BKreitman%2C+Robert+J%3BPastan%2C+Ira&rft.aulast=Onda&rft.aufirst=Masanori&rft.date=2005-08-01&rft.volume=11&rft.issue=16&rft.spage=5840&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Cell surface; Fc; Western blotting; Ovarian cancer; Enzyme-linked immunosorbent assay; Data processing; Monoclonal antibodies; Immunotherapy; Tumors; Plasmids; Cell fusion; Metastases; Flow cytometry; Hybridoma; Immunoglobulin G; Fusion protein; Adhesives; Immunohistochemistry; Manganese; Epitopes ER - TY - JOUR T1 - Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome AN - 20236866; 6703324 AB - The organization of eukaryotic genomes into distinct structural and functional domains is important for the regulation and transduction of genetic information. Here, we investigated heterochromatin and euchromatin profiles of the entire fission yeast genome and explored the role of RNA interference (RNAi) in genome organization. Histone H3 methylated at Lys4, which defines euchromatin, was not only distributed across most of the chromosomal landscape but was also present at the centromere core, the site of kinetochore assembly. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Notably, RNAi components were distributed throughout all these heterochromatin domains, and their localization depended on Clr4/Suv39h histone methyltransferase. Sequencing of small interfering RNAs (siRNAs) associated with the RITS RNAi effector complex identified hot spots of siRNAs, which mapped to a diverse array of elements in these RNAi-heterochromatin domains. We found that Clr4/Suv39h predominantly silenced repeat elements whose derived transcripts, transcribed mainly by RNA polymerase II, serve as a source for siRNAs. Our analyses also uncover an important role for the RNAi machinery in maintaining genomic integrity. JF - Nature Genetics AU - Cam, Hugh P AU - Sugiyama, Tomoyasu AU - Chen, Ee Sin AU - Chen, Xi AU - FitzGerald, Peter C AU - Grewal, Shiv I S AD - Laboratory of Molecular Cell Biology, National Institutes of Health, Bethesda, Maryland 20892-4255, USA., grewals@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 809 EP - 819 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 37 IS - 8 SN - 1061-4036, 1061-4036 KW - fission yeast KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology; Genetics Abstracts KW - Genomes KW - Euchromatin KW - Heterochromatin KW - Hot spots KW - Landscape KW - Centromeres KW - DNA-directed RNA polymerase KW - Islands KW - siRNA KW - Cores KW - epigenetics KW - Meiosis KW - Structure-function relationships KW - Kinetochores KW - histone methyltransferase KW - RNA-mediated interference KW - genomics KW - Histone H3 KW - Schizosaccharomyces pombe KW - K 03015:Fungi KW - G 07210:Cell cycle KW - W 30940:Products KW - N 14820:DNA Metabolism & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20236866?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Genetics&rft.atitle=Comprehensive+analysis+of+heterochromatin-+and+RNAi-mediated+epigenetic+control+of+the+fission+yeast+genome&rft.au=Cam%2C+Hugh+P%3BSugiyama%2C+Tomoyasu%3BChen%2C+Ee+Sin%3BChen%2C+Xi%3BFitzGerald%2C+Peter+C%3BGrewal%2C+Shiv+I+S&rft.aulast=Cam&rft.aufirst=Hugh&rft.date=2005-08-01&rft.volume=37&rft.issue=8&rft.spage=809&rft.isbn=&rft.btitle=&rft.title=Nature+Genetics&rft.issn=10614036&rft_id=info:doi/10.1038%2Fng1602 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Genomes; Euchromatin; Heterochromatin; Hot spots; Landscape; Centromeres; DNA-directed RNA polymerase; Islands; Cores; siRNA; Kinetochores; Structure-function relationships; Meiosis; epigenetics; histone methyltransferase; RNA-mediated interference; genomics; Histone H3; Schizosaccharomyces pombe DO - http://dx.doi.org/10.1038/ng1602 ER - TY - JOUR T1 - Methanococcus vannielii selenium-binding protein (SeBP): Chemical reactivity of recombinant SeBP produced in Escherichia coli AN - 20197475; 6519303 AB - A selenium-binding protein (SeBP) from Methanococcus vannielii was recently identified, and its gene was isolated and overexpressed in Escherichia coli [Self, W. T., Pierce, R. & Stadtman, T. C. (2004) IUBMB Life 56, 501-507]. SeBP and recombinant SeBP (rSeBP) migrated as approximately 42-kDa species on native gels and as approximately 33-kDa species on SDS gels. rSeBP consists of identical 8.8-kDa subunits, each containing a single cysteine residue. rSeBP isolated in the absence of reducing agents contained oxidized cysteine (89%) and very little bound selenium (0.05 eq or less per subunit). Complete reduction of the oxidized cysteine residues in rSeBP with Tris(2-carboxyethyl)phosphine required addition of a denaturant, such as 1 M guanidine-hydrochloride. With selenite as the selenium source and the isolated reduced protein as sole reductant, binding of one selenium per tetramer under anaerobic conditions required four cysteine thiol groups, one on each subunit. In the corresponding reaction, with reduced glutathione (GSH), equimolar amounts of selenodiglutathione (GSSeSG) and glutathione disulfide are formed from selenite and 4 GSH. At GSH-to-selenite ratios >4:1, conversion of GSSeSG to a perselenide derivative, GSSe super(-), occurs. However, with the reduced rSeBP as sole electron donor in the reaction with selenite, further conversion of the R-SSeS-R product apparently did not occur. Prior alkylation of the cysteine thiol groups in reduced rSeBP prevented selenite reduction and selenium binding under comparable conditions. JF - Proceedings of the National Academy of Sciences, USA AU - Patteson, Kemberly G AU - Trivedi, Neel AU - Stadtman, Thressa C AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8012 Y1 - 2005/08// PY - 2005 DA - August 2005 SP - 12029 EP - 12034 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 34 SN - 0027-8424, 0027-8424 KW - selenium-binding protein KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Oceanic Abstracts; Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Glutathione KW - selenite KW - Metabolites KW - Alkylation KW - Methanococcus vannielii KW - Protein structure KW - Gels KW - Recombinants KW - Selenium KW - Methanococcus KW - Anoxic conditions KW - Cysteine KW - Thiols KW - Reducing agents KW - Escherichia coli KW - Proteins KW - Coenzymes KW - Chemical modification KW - Q5 08503:Characteristics, behavior and fate KW - Q1 08421:Migrations and rhythms KW - G 07320:Bacterial genetics KW - O 4080:Pollution - Control and Prevention KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20197475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Methanococcus+vannielii+selenium-binding+protein+%28SeBP%29%3A+Chemical+reactivity+of+recombinant+SeBP+produced+in+Escherichia+coli&rft.au=Patteson%2C+Kemberly+G%3BTrivedi%2C+Neel%3BStadtman%2C+Thressa+C&rft.aulast=Patteson&rft.aufirst=Kemberly&rft.date=2005-08-01&rft.volume=102&rft.issue=34&rft.spage=12029&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Recombinants; Gels; Selenium; Anoxic conditions; Cysteine; Proteins; Metabolites; Coenzymes; Protein structure; Glutathione; Reducing agents; Thiols; selenite; Alkylation; Chemical modification; Methanococcus vannielii; Methanococcus; Escherichia coli ER - TY - JOUR T1 - A catalog for the transcripts from the venomous structures of the caterpillar Lonomia obliqua: Identification of the proteins potentially involved in the coagulation disorder and hemorrhagic syndrome AN - 20190806; 6501415 AB - Accidents with the caterpillar Lonomia obliqua are often associated with a coagulation disorder and hemorrhagic syndrome in humans. In the present study, we have constructed cDNA libraries from two venomous structures of the caterpillar, namely the tegument and the bristle. High-throughput sequencing and bioinformatics analyses were performed in parallel. Over one thousand cDNAs were obtained and clustered to produce a database of 538 contigs and singletons (clusters) for the tegument library and 368 for the bristle library. We have thus identified dozens of full-length cDNAs coding for proteins with sequence homology to snake venom prothrombin activator, trypsin-like enzymes, blood coagulation factors and prophenoloxidase cascade activators. We also report cDNA coding for cysteine proteases, Group III phospholipase A2, C-type lectins, lipocalins, in addition to protease inhibitors including serpins, Kazal-type inhibitors, cystatins and trypsin inhibitor-like molecules. Antibacterial proteins and housekeeping genes are also described. A significant number of sequences were devoid of database matches, suggesting that their biologic function remains to be defined. We also report the N-terminus of the most abundant proteins present in the bristle, tegument, hemolymph, and ''cryosecretion''. Thus, we have created a catalog that contains the predicted molecular weight, isoelectric point, accession number, and putative function for each selected molecule from the venomous structures of L. obliqua. The role of these molecules in the coagulation disorder and hemorrhagic syndrome caused by envenomation with this caterpillar is discussed. All sequence information and the Supplemental Data, including figures and tables with hyperlinks to FASTA-formatted files for each contig and the best match to the databases, are available at http:/ /www.ncbi.nih.gov/projects/omes. JF - Gene AU - Veiga, ABG AU - Ribeiro, JMC AU - Guimaraes, JA AU - Francischetti, IMB AD - Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, 12735 Twinbrook Parkway, Twinbrook III, Room 2E-28, Rockville, MD 20892-8132, USA, ifrancischetti@niaid.nih.gov Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 11 EP - 27 PB - Elsevier B.V. VL - 355 SN - 0378-1119, 0378-1119 KW - Microbiology Abstracts B: Bacteriology; Entomology Abstracts; Genetics Abstracts KW - Bristles KW - Catalogs KW - Hemorrhage KW - N-Terminus KW - Lonomia obliqua KW - Accidents KW - Molecular weight KW - prophenoloxidase KW - Lipocalin KW - serpins KW - Cysteine proteinase KW - Hemolymph KW - Isoelectric points KW - Data processing KW - Tegument KW - Trypsin KW - Phospholipase A2 KW - Proteinase inhibitors KW - Enzymes KW - Lectins KW - Cystatin KW - Databases KW - Blood coagulation KW - prothrombin KW - Homology KW - Bioinformatics KW - Venom KW - G 07480:Hematological disorders KW - Z 05320:Physiology, Anatomy, and Biochemistry KW - J 02340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20190806?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene&rft.atitle=A+catalog+for+the+transcripts+from+the+venomous+structures+of+the+caterpillar+Lonomia+obliqua%3A+Identification+of+the+proteins+potentially+involved+in+the+coagulation+disorder+and+hemorrhagic+syndrome&rft.au=Veiga%2C+ABG%3BRibeiro%2C+JMC%3BGuimaraes%2C+JA%3BFrancischetti%2C+IMB&rft.aulast=Veiga&rft.aufirst=ABG&rft.date=2005-08-01&rft.volume=355&rft.issue=&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Gene&rft.issn=03781119&rft_id=info:doi/10.1016%2Fj.gene.2005.05.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Bristles; Catalogs; Hemorrhage; N-Terminus; Accidents; Molecular weight; prophenoloxidase; Lipocalin; serpins; Cysteine proteinase; Hemolymph; Isoelectric points; Data processing; Trypsin; Tegument; Phospholipase A2; Proteinase inhibitors; Enzymes; Lectins; Databases; Cystatin; Blood coagulation; prothrombin; Homology; Bioinformatics; Venom; Lonomia obliqua DO - http://dx.doi.org/10.1016/j.gene.2005.05.002 ER - TY - JOUR T1 - Crystal Structure of the Bacterial YhcH Protein Indicates a Role in Sialic Acid Catabolism AN - 20152103; 6517733 AB - The yhcH gene is part of the nan operon in bacteria that encodes proteins involved in sialic acid catabolism. Determination of the crystal structure of YhcH from Haemophilus influenzae was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. The structure was determined at 2.2-Aa resolution by multiple-wavelength anomalous diffraction. The protein fold is a variation of the double-stranded beta -helix. Two antiparallel beta -sheets form a funnel opened at one side, where a putative active site contains a copper ion coordinated to the side chains of two histidine and two carboxylic acid residues. A comparison to other proteins with a similar fold and analysis of the genomic context suggested that YhcH may be a sugar isomerase involved in processing of exogenous sialic acid. JF - Journal of Bacteriology AU - Teplyakov, Alexey AU - Obmolova, Galina AU - Toedt, John AU - Galperin, Michael Y AU - Gilliland, Gary L AD - Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute and National Institute of Standards and Technology, Rockville, Maryland. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5520 EP - 5527 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - YhcH protein KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Bacteria KW - Sugar KW - Haemophilus influenzae KW - Copper KW - Protein folding KW - Histidine KW - Crystal structure KW - carboxylic acids KW - genomics KW - Diffraction KW - Operons KW - Sialic acids KW - G 07320:Bacterial genetics KW - A 01490:Miscellaneous KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20152103?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Crystal+Structure+of+the+Bacterial+YhcH+Protein+Indicates+a+Role+in+Sialic+Acid+Catabolism&rft.au=Teplyakov%2C+Alexey%3BObmolova%2C+Galina%3BToedt%2C+John%3BGalperin%2C+Michael+Y%3BGilliland%2C+Gary+L&rft.aulast=Teplyakov&rft.aufirst=Alexey&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5520&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Sugar; Protein folding; Histidine; carboxylic acids; Crystal structure; Copper; Diffraction; genomics; Operons; Sialic acids; Bacteria; Haemophilus influenzae ER - TY - JOUR T1 - The efficacy of mesenchymal stem cells to regenerate and repair dental structures AN - 19991725; 6627819 AB - Authors -: Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S Objectives -: Identification, characterization, and potential application of mesenchymal stem cells (MSC) derived from human dental tissues. Methods -: Dental pulp and periodontal ligament were obtained from normal human impacted third molars. The tissues were digested in collagenase/dispase to generate single cell suspensions. Cells were cultured in alpha -MEM supplemented with 20% fetal bovine serum, 2 mM l-glutamine, 100 mu M l-ascorbate-2-phosphate. Magnetic and fluorescence activated cell sorting were employed to characterize the phenotype of freshly isolated and ex vivo expanded cell populations. The developmental potential of cultured cells was assessed following co-transplantation with hydroxyapetite/tricalcium phosphate (HA/TCP) particles into immunocompromised mice for 8 weeks. Results -: MSC were identified in adult human dental pulp (dental pulp stem cells, DPSC), human primary teeth (stem cells from human exfoliated deciduous teeth, SHED), and periodontal ligament (periodontal ligament stem cells, PDLSC) by their capacity to generate clongenic cell clusters in culture. Ex vivo expanded DPSC, SHED, and PDLSC populations expressed a heterogeneous assortment of makers associated with MSC, dentin, bone, smooth muscle, neural tissue, and endothelium. PDLSC were also found to express the tendon specific marker, Scleraxis. Xenogeneic transplants containing HA/TCP with either DPSC or SHED generated donor-derived dentin-pulp-like tissues with distinct odontoblast layers lining the mineralized dentin-matrix. In parallel studies, PDLSC generated cementum-like structures associated with PDL-like connective tissue when transplanted with HA/TCP into immunocompromised mice. Conclusion -: Collectively, these data revealed the presence of distinct MSC populations associated with dental structures with the potential of stem cells to regenerate living human dental tissues in vivo. JF - Orthodontics and Craniofacial Research AU - Shi, S AU - Bartold, P M AU - Miura, M AU - Seo, B M AU - Robey, P G AU - Gronthos, S AD - S. Shi, M. Miura, B.M. Seo, P.G. Robey, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, MD, USA P.M. Bartold, Colgate Australian Clinical Dental Research Centre, Dental School, University of Adelaide, Adelaide Dental Hospital, Adelaide, SA, Australia S. Gronthos, Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, SA, Australia, stan.gronthos@imvs.sa.gov.au Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 191 EP - 199 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 8 IS - 3 SN - 1601-6335, 1601-6335 KW - Biotechnology and Bioengineering Abstracts; Calcium & Calcified Tissue Abstracts KW - Odontoblasts KW - Teeth KW - Smooth muscle KW - Cell suspensions KW - Dentin KW - Glutamine KW - Data processing KW - Collagenase KW - Connective tissues KW - Molars KW - Cell culture KW - Bone KW - Flow cytometry KW - Stem cells KW - periodontal ligament KW - Endothelium KW - Mesenchyme KW - tricalcium phosphate KW - Tendons KW - Dental pulp KW - T 2045:Teeth KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19991725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Orthodontics+and+Craniofacial+Research&rft.atitle=The+efficacy+of+mesenchymal+stem+cells+to+regenerate+and+repair+dental+structures&rft.au=Shi%2C+S%3BBartold%2C+P+M%3BMiura%2C+M%3BSeo%2C+B+M%3BRobey%2C+P+G%3BGronthos%2C+S&rft.aulast=Shi&rft.aufirst=S&rft.date=2005-08-01&rft.volume=8&rft.issue=3&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Orthodontics+and+Craniofacial+Research&rft.issn=16016335&rft_id=info:doi/10.1111%2Fj.1601-6343.2005.00331.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - SuppNotes - Figures, 1; tables, 1. N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Cell suspensions; Smooth muscle; Teeth; Odontoblasts; Dentin; Glutamine; Data processing; Molars; Connective tissues; Collagenase; Cell culture; Flow cytometry; Bone; Stem cells; periodontal ligament; Endothelium; Mesenchyme; Tendons; tricalcium phosphate; Dental pulp DO - http://dx.doi.org/10.1111/j.1601-6343.2005.00331.x ER - TY - JOUR T1 - Toward a live microbial microbicide for HIV: Commensal bacteria secreting an HIV fusion inhibitor peptide AN - 19936109; 6519297 AB - Most HIV transmission occurs on the mucosal surfaces of the gastrointestinal and cervicovaginal tracts, both of which are normally coated by a biofilm of nonpathogenic commensal bacteria. We propose to genetically engineer such naturally occurring bacteria to protect against HIV infection by secreting antiviral peptides. Here we describe the development and characterization of Nissle 1917, a highly colonizing probiotic strain of Escherichia coli, secreting HIV-gp41-hemolysin A hybrid peptides that block HIV fusion and entry into target cells. By using an appropriate combination of cis- and transacting secretory and regulatory signals, micromolar secretion levels of the anti-HIV peptides were achieved. The genetically engineered Nissle 1917 were capable of colonizing mice for periods of weeks to months, predominantly in the colon and cecum, with lower concentrations of bacteria present in the rectum, vagina, and small intestine. Histological and immunocytochemical examination of the colon revealed bacterial growth and peptide secretion throughout the luminal mucosa and in association with epithelial surfaces. The use of genetically engineered live microbes as anti-HIV microbicides has important potential advantages in economy, efficacy, and durability. JF - Proceedings of the National Academy of Sciences, USA AU - Rao, Srinivas AU - Hu, Stella AU - McHugh, Louise AU - Lueders, Kira AU - Henry, Ken AU - Zhao, Qi AU - Fekete, Richard A AU - Kar, Sudeshna AU - Adhya, Sankar AU - Hamer, Dean H AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Laboratories of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 11993 EP - 11998 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 34 SN - 0027-8424, 0027-8424 KW - HIV KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Genetics Abstracts; Biotechnology and Bioengineering Abstracts; Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology; Medical and Pharmaceutical Biotechnology Abstracts KW - Rectum KW - glycoprotein gp41 KW - Mucosa KW - probiotics KW - Commensals KW - Small intestine KW - Infection KW - Disease transmission KW - Antiviral agents KW - Colon KW - Human immunodeficiency virus KW - Genetic engineering KW - Hybrids KW - Vagina KW - Escherichia coli KW - Cecum KW - Biofilms KW - microbicides KW - A 01340:Antibiotics & Antimicrobials KW - J 02732:Other cell constituents and metabolites KW - V 22002:AIDS: Molecular and in vitro aspects KW - W3 33372:Antiviral agents KW - W 30965:Miscellaneous, Reviews KW - G 07770:Bacteria KW - J 02740:Genetics and evolution KW - J 02812:Antibacterial Agents: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19936109?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.genre=dissertations+%26+theses&rft.jtitle=&rft.atitle=&rft.au=Ayvazian%2C+John&rft.aulast=Ayvazian&rft.aufirst=John&rft.date=1996-09-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Parenting+styles+in+the+Armenian+family&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Rectum; glycoprotein gp41; Mucosa; Commensals; probiotics; Small intestine; Infection; Disease transmission; Colon; Antiviral agents; Hybrids; Genetic engineering; Vagina; Cecum; Biofilms; microbicides; Human immunodeficiency virus; Escherichia coli ER - TY - JOUR T1 - An anti-HIV microbicide comes alive AN - 19934422; 6519350 AB - Topical microbicides are a broad class of agents designed to block or kill infectious microorganisms directly at the site of transmission. With the AIDS pandemic continuing its unrelenting global march (40 million current infections, 14,000 new infections per day) driven largely by sexual transmission, microbicides have moved steadily toward the front line of preventative strategies. Indeed, many candidate anti-HIV microbicides are currently under development, with several already in clinical trials. A battery of promising protein-based HIV inhibitors can potentially be developed, but they face serious challenges of high production costs and instability during transport and storage. In a recent issue of PNAS, Rao et al. presented an intriguing version of a "live microbicide" approach whereby a commensal bacterium is engineered to secrete a potent anti-HIV peptide. When administered orally or as a rectal suppository, the bacteria would colonize the gut mucosa and secrete the peptide in situ, thereby providing protection in advance of exposure hopefully for days, weeks, or even months. This delivery mode would be highly advantageous over others requiring repeated topical application before each act of intercourse; also, the engineered bacteria would be relatively simple and inexpensive to manufacture, transport, and store. JF - Proceedings of the National Academy of Sciences, USA AU - Lagenaur, Laurel A AU - Berger, Edward A AD - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 12294 EP - 12295 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 35 SN - 0027-8424, 0027-8424 KW - HIV KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Virology & AIDS Abstracts KW - Acquired immune deficiency syndrome KW - Rectum KW - Mucosa KW - Commensals KW - Infection KW - Clinical trials KW - Topical application KW - Disease transmission KW - pandemics KW - Digestive tract KW - Human immunodeficiency virus KW - Microorganisms KW - microbicides KW - V 22002:AIDS: Molecular and in vitro aspects KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19934422?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologya&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=An+anti-HIV+microbicide+comes+alive&rft.au=Lagenaur%2C+Laurel+A%3BBerger%2C+Edward+A&rft.aulast=Lagenaur&rft.aufirst=Laurel&rft.date=2005-08-01&rft.volume=102&rft.issue=35&rft.spage=12294&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - pandemics; Acquired immune deficiency syndrome; Rectum; Digestive tract; Mucosa; Microorganisms; Commensals; Infection; Clinical trials; Disease transmission; Topical application; microbicides; Human immunodeficiency virus ER - TY - JOUR T1 - A quantitative determination of multi-protein interactions by the analysis of confocal images using a pixel-by-pixel assessment algorithm AN - 19808756; 6480790 AB - MOTIVATION: Recent advances in confocal microscopy have allowed scientists to assess the expression, and to some extent, the interaction/colocalization of multiple molecules within cells and tissues. In some instances, accurately quantifying the colocalization of two or more proteins may be critical. This can require the acquisition of multiple Z plane images (Z stacks) throughout a specimen and, as such, we report here the successful development of a freeware, open-source image analysis tool, IMAJIN_COLOC, developed in PERL (v. 5.8, build 806), using the PERLMagick libraries (ImageMagick). Using a pixel-by-pixel analysis algorithm, IMAJIN_COLOC can analyze images for antigen expression (any number of colors) and can measure all possible combinations of colocalization for up to three colors by analyzing a Z stack gallery acquired for each sample. The simultaneous (i.e. in a single pass) analysis of three-color colocalization, and batch analysis capabilities are distinctive features of this program. RESULTS: A control image, containing known individual and colocalized pixel counts, was used to validate the accuracy of IMAJIN_COLOC. As further validation, pixel counts and colocalization values from the control image were compared to those obtained with the software packaged with the Zeiss laser-scanning microscope (LSM AIM, version 3.2). The values from both programs were found to be identical. To demonstrate the applicability of this program in addressing novel biological questions, we examined the role of neurons in eliciting an immune reaction in response to viral infection. Specifically, we successfully examined expression of the chemokine RANTES in measles virus (MV) infected hippocampal neurons and quantified changes in RANTES production throughout the disease period. The resultant quantitative data were also evaluated visually, using a gif image created during the analysis. AVAILABILITY: PERL (ActivePerl, version 5.8) is available at activestate.com; the PERLMagick libraries are available at imagemagick.org, and IMAJIN_COLOC, the source code and user documentation can be downloaded from http://www.fda.gov/cber/research/imaging/imageanalysis.htm JF - Bioinformatics AU - Goucher AU - Wincovitch, S M AU - Garfield, SH AU - Carbone, K M AU - Malik, TH AD - DVP/OVRR, Center for Biologics Evaluation and Research, US Food and Drug Administration Bethesda, MD 20892, USA. Laboratory of Experimental Carcinogenesis, National Cancer Institute Bethesda, MD 20892, USA. Department of Psychiatry and Department of Medicine, Johns Hopkins University Baltimore, MD, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 3248 EP - 3254 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 15 SN - 1367-4803, 1367-4803 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts KW - Galleries KW - Chemokines KW - Data processing KW - Hippocampus KW - Microscopes KW - Algorithms KW - RANTES KW - Image processing KW - Measles virus KW - Infection KW - Color KW - Computer programs KW - software KW - Neurons KW - Confocal microscopy KW - Bioinformatics KW - V 22320:Replication KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19808756?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=The+News+Tribune&rft.atitle=Book+ban+appeal+goes+to+FWay+board+%27Catcher+in+the+Rye%27%3A+Jefferson+High+parent+protests+often-+challenged+novel%3A+%5BSouth+Sound+Edition%5D&rft.au=Robinson%2C+Sean&rft.aulast=Robinson&rft.aufirst=Sean&rft.date=2001-07-17&rft.volume=&rft.issue=&rft.spage=B.1&rft.isbn=&rft.btitle=&rft.title=The+News+Tribune&rft.issn=10735860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Galleries; Chemokines; Data processing; Hippocampus; Microscopes; Algorithms; Image processing; RANTES; Infection; Color; Computer programs; software; Neurons; Confocal microscopy; Bioinformatics; Measles virus ER - TY - JOUR T1 - Escherichia coli K1's Capsule Is a Barrier to Bacteriophage T7 AN - 19443084; 6520443 AB - Escherichia coli strains that produce the K1 polysaccharide capsule have long been associated with pathogenesis. This capsule is believed to increase the cell's invasiveness, allowing the bacteria to avoid phagocytosis and inactivation by complement. It is also recognized as a receptor by some phages, such as K1F and K1-5, which have virion-associated enzymes that degrade the polysaccharide. In this report we show that expression of the K1 capsule in E. coli physically blocks infection by T7, a phage that recognizes lipopolysaccharide as the primary receptor. Enzymatic removal of the K1 antigen from the cell allows T7 to adsorb and replicate. This observation suggests that the capsule plays an important role as a defense against some phages that recognize structures beneath it and that the K1-specific phages evolved to counter this physical barrier. JF - Applied and Environmental Microbiology AU - Scholl, Dean AU - Adhya, Sankar AU - Merril, Carl AD - Section of Biochemical Genetics, The National Institute of Mental Health, NIH, Building 36, Room 3D20, 9000 Rockville Pike, Bethesda, Maryland 20892. Section of Developmental Genetics, The National Cancer Institute, NIH, Building 37, Room 5138C, 9000 Rockville Pike, Bethesda, Maryland 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4872 EP - 4874 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 71 IS - 8 SN - 0099-2240, 0099-2240 KW - Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - Phages KW - Capsules KW - Invasiveness KW - Barriers KW - Complement KW - Escherichia coli KW - Lipopolysaccharides KW - Polysaccharides KW - Infection KW - Phagocytosis KW - J 02750:Phage-host interactions KW - V 22070:Phage-host interactions including lysogeny & transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19443084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=Fort+Worth+Star+-+Telegram&rft.atitle=%27Rye%27+relevance+50+years+ago%2C+J.D.+Salinger+put+a+face+on+teen+angst+and+gave+it+credibility.+Since+then%2C+his+%27Catcher+in+the+Rye%27+has+become+a+multimedia+mega-force%2C+influencing+books%2C+movies%2C+music+and+television.%3A+%5BFINAL+Edition%5D&rft.au=Guinn%2C+Jeff&rft.aulast=Guinn&rft.aufirst=Jeff&rft.date=2001-08-05&rft.volume=&rft.issue=&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Fort+Worth+Star+-+Telegram&rft.issn=08890013&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Phages; Capsules; Invasiveness; Barriers; Complement; Lipopolysaccharides; Phagocytosis; Infection; Polysaccharides; Escherichia coli ER - TY - JOUR T1 - Prediction error estimation: a comparison of resampling methods AN - 19427956; 6480797 AB - MOTIVATION: In genomic studies, thousands of features are collected on relatively few samples. One of the goals of these studies is to build classifiers to predict the outcome of future observations. There are three inherent steps to this process: feature selection, model selection and prediction assessment. With a focus on prediction assessment, we compare several methods for estimating the 'true' prediction error of a prediction model in the presence of feature selection. RESULTS: For small studies where features are selected from thousands of candidates, the resubstitution and simple split-sample estimates are seriously biased. In these small samples, leave-one-out cross-validation (LOOCV), 10-fold cross-validation (CV) and the .632+ bootstrap have the smallest bias for diagonal discriminant analysis, nearest neighbor and classification trees. LOOCV and 10-fold CV have the smallest bias for linear discriminant analysis. Additionally, LOOCV, 5- and 10-fold CV, and the .632+ bootstrap have the lowest mean square error. The .632+ bootstrap is quite biased in small sample sizes with strong signal-to-noise ratios. Differences in performance among resampling methods are reduced as the number of specimens available increase. CONTACT: annette.molinaroale.edu Supplementary Information: A complete compilation of results and R code for simulations and analyses are available in Molinaro et al. (2005) (http://linus.nci.nih.gov/brb/TechReport.htm). JF - Bioinformatics AU - Molinaro, Annette M AU - Simon, Richard AU - Pfeiffer, Ruth M AD - Biostatistics Branch, Division of Cancer Epidemiology and Genetics, NCI, NIH Rockville, MD 20852 USA. Biometric Research Branch, Division of Cancer Treatment and Diagnostics, NCI, NIH Rockville, MD 20852 USA. Department of Epidemiology and Public Health, Yale University School of Medicine New Haven, CT 06520, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 3301 EP - 3307 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 15 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts KW - Bioinformatics KW - genomics KW - Models KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19427956?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Prediction+error+estimation%3A+a+comparison+of+resampling+methods&rft.au=Molinaro%2C+Annette+M%3BSimon%2C+Richard%3BPfeiffer%2C+Ruth+M&rft.aulast=Molinaro&rft.aufirst=Annette&rft.date=2005-08-01&rft.volume=21&rft.issue=15&rft.spage=3301&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Models; genomics; Bioinformatics ER - TY - JOUR T1 - Prostate Cancer: Correlation of MR Imaging and MR Spectroscopy with Pathologic Findings after Radiation Therapy-Initial Experience AN - 19420999; 6477695 AB - PURPOSE: To prospectively evaluate magnetic resonance (MR) imaging and MR spectroscopy for depiction of local prostate cancer recurrence after external-beam radiation therapy, with step-section pathologic findings as the standard of reference. MATERIALS AND METHODS: Study received institutional approval, and written informed consent was obtained. Study was compliant with Health Insurance Portability and Accountability Act. Sextant biopsy, digital rectal examination, MR imaging, MR spectroscopy, and salvage radical prostatectomy with step-section pathologic examination were performed in nine patients with increasing prostate-specific antigen levels after external-beam radiation therapy. MR imaging criterion for tumor was a focal nodular region of reduced signal intensity at T2-weighted imaging. MR spectroscopic criteria for tumor were voxels with choline (Cho) plus creatine (Cr) to citrate (Cit) ratio ([Cho + Cr]/Cit) of at least 0.5 or voxels with detectable Cho and no Cit in the peripheral zone. Sensitivity and specificity of sextant biopsy, digital rectal examination, MR imaging, and MR spectroscopy were determined by using a prostate sextant as the unit of analysis. For feature analysis, MR imaging and MR spectroscopic findings were correlated with step-section pathologic findings. RESULTS: MR imaging and MR spectroscopy showed estimated sensitivities of 68% and 77%, respectively, while sensitivities of biopsy and digital rectal examination were 48% and 16%, respectively. MR spectroscopy appears to be less specific (78%) than the other three tests, each of which had a specificity higher than 90%. MR spectroscopic feature analysis showed that a metabolically altered benign gland could be falsely identified as tumor by using MR spectroscopic criteria; further analysis of MR spectroscopic features did not lead to improved MR spectroscopic criteria for recurrent tumor. CONCLUSION: In summary, MR imaging and MR spectroscopy may be more sensitive than sextant biopsy and digital rectal examination for sextant localization of cancer recurrence after external-beam radiation therapy. [copy ] RSNA, 2005 JF - Radiology AU - Pucar, Darko AU - Shukla-Dave, Amita AU - Hricak, Hedvig AU - Moskowitz, Chaya S AU - Kuroiwa, Kentaro AU - Olgac, Semra AU - Ebora, Lanie E AU - Scardino, Peter T AU - Koutcher, Jason A AU - Zakian, Kristen L AD - Departments of Radiology (D.P., A.S.D., H.H., L.E., J.A.K., K.L.Z.), Medical Physics (D.P., A.S.D., L.E., J.A.K., K.L.Z.), Epidemiology and Biostatistics (C.M.), Urology (K.K., P.T.S.), Pathology (K.K., S.O.), and Medicine (J.A.K.), Memorial Sloan-Kettering Cancer Center, 1275 York Ave, New York, NY 10021. Supported by National Institutes of Health grant R01 CA76423 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 545 EP - 553 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 236 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts KW - Choline KW - Rectum KW - Magnetic resonance imaging KW - Creatine KW - Biopsy KW - Tumors KW - prostate-specific antigen KW - Cancer KW - Prostate cancer KW - Radiation KW - Glands KW - Magnetic resonance spectroscopy KW - Radicals KW - Citric acid KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420999?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Prostate+Cancer%3A+Correlation+of+MR+Imaging+and+MR+Spectroscopy+with+Pathologic+Findings+after+Radiation+Therapy-Initial+Experience&rft.au=Pucar%2C+Darko%3BShukla-Dave%2C+Amita%3BHricak%2C+Hedvig%3BMoskowitz%2C+Chaya+S%3BKuroiwa%2C+Kentaro%3BOlgac%2C+Semra%3BEbora%2C+Lanie+E%3BScardino%2C+Peter+T%3BKoutcher%2C+Jason+A%3BZakian%2C+Kristen+L&rft.aulast=Pucar&rft.aufirst=Darko&rft.date=2005-08-01&rft.volume=236&rft.issue=2&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Magnetic resonance imaging; Magnetic resonance spectroscopy; Biopsy; Radiation; Rectum; Tumors; Prostate cancer; Glands; Citric acid; Creatine; Radicals; prostate-specific antigen; Cancer; Choline ER - TY - JOUR T1 - BioMart and Bioconductor: a powerful link between biological databases and microarray data analysis AN - 19420375; 6522682 AB - SUMMARY: biomaRt is a new Bioconductor package that integrates BioMart data resources with data analysis software in Bioconductor. It can annotate a wide range of gene or gene product identifiers (e.g. Entrez-Gene and Affymetrix probe identifiers) with information such as gene symbol, chromosomal coordinates, Gene Ontology and OMIM annotation. Furthermore biomaRt enables retrieval of genomic sequences and single nucleotide polymorphism information, which can be used in data analysis. Fast and up-to-date data retrieval is possible as the package executes direct SQL queries to the BioMart databases (e.g. Ensembl). The biomaRt package provides a tight integration of large, public or locally installed BioMart databases with data analysis in Bioconductor creating a powerful environment for biological data mining. AVAILABILITY: http://www.bioconductor.org. LGPL CONTACT: steffen.durincksat.kuleuven.ac.be JF - Bioinformatics AU - Durinck, Steffen AU - Moreau, Yves AU - Kasprzyk, Arek AU - Davis, Sean AU - De Moor, Bart AU - Brazma, Alvis AU - Huber, Wolfgang AD - Department of Electronical Engineering ESAT-SCD, K.U.Leuven, Kasteelpark Arenberg 10, 3001 Leuven-Heverlee, Belgium. EBI, Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SD, UK. Cancer Genetics Branch, National Human Genome Research Institute, National Institute of Health 50 South Drive, Bethesda, MD 20892-8000, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3439 EP - 3440 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 16 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts KW - Integration KW - Computer programs KW - Databases KW - software KW - Data processing KW - Single-nucleotide polymorphism KW - DNA probes KW - genomics KW - Bioinformatics KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420375?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=BioMart+and+Bioconductor%3A+a+powerful+link+between+biological+databases+and+microarray+data+analysis&rft.au=Durinck%2C+Steffen%3BMoreau%2C+Yves%3BKasprzyk%2C+Arek%3BDavis%2C+Sean%3BDe+Moor%2C+Bart%3BBrazma%2C+Alvis%3BHuber%2C+Wolfgang&rft.aulast=Durinck&rft.aufirst=Steffen&rft.date=2005-08-01&rft.volume=21&rft.issue=16&rft.spage=3439&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Data processing; Databases; Bioinformatics; genomics; Computer programs; software; Integration; Single-nucleotide polymorphism; DNA probes ER - TY - JOUR T1 - Optimizing brain tissue contrast with EPI: A simulated annealing approach AN - 19418625; 6489828 AB - A new magnetization preparation and image acquisition scheme was developed to obtain high-resolution brain images with optimal tissue contrast. The pulse sequence was derived from an optimization process using simulated annealing, without prior assumptions with regard to the number of radiofrequency (RF) pulses and flip angles. The resulting scheme combined two inversion pulses with the acquisition of three images with varying contrast. The combination of the three images allowed separation of gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) based on T sub(1), contrast. It also enabled the correction of small errors in the initial T sub(1) estimates in postprocessing. The use of three-dimensional (3D) sensitivity-encoded (SENSE) echo-planar imaging (EPI) for image acquisition made it possible to achieve a 1.15 super(3) mm super(3) isotropic resolution within a scan time of 10 min 21 s. The cortical GM signal-to-noise ratio (SNR) in the calculated GM-only image varied between 30 and 100. The novel technique was evaluated in combination with blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI) on human subjects, and provided for excellent coregistration of anatomical and functional data. JF - Magnetic Resonance in Medicine AU - Ikonomidou, Vasiliki N AU - Van Gelderen, Peter AU - De Zwart, Jacco A AU - Fukunaga, Masaki AU - Duyn, Jeff H AD - Advanced MRI Section, LFMI, NINDS, National Institutes of Health, Bldg. 10, Rm. B1D-722, MSC 1065, 9000 Rockville Pike, Bethesda, MD 20892-1065, USA, viko@nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 373 EP - 385 PB - John Wiley & Sons, Ltd. VL - 54 IS - 2 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Cerebrospinal fluid KW - Inversion KW - Functional magnetic resonance imaging KW - Brain KW - Substantia alba KW - N.M.R. KW - Substantia grisea KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19418625?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Optimizing+brain+tissue+contrast+with+EPI%3A+A+simulated+annealing+approach&rft.au=Ikonomidou%2C+Vasiliki+N%3BVan+Gelderen%2C+Peter%3BDe+Zwart%2C+Jacco+A%3BFukunaga%2C+Masaki%3BDuyn%2C+Jeff+H&rft.aulast=Ikonomidou&rft.aufirst=Vasiliki&rft.date=2005-08-01&rft.volume=54&rft.issue=2&rft.spage=373&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20561 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Functional magnetic resonance imaging; Cerebrospinal fluid; Brain; Neuroimaging; N.M.R.; Substantia alba; Inversion; Substantia grisea DO - http://dx.doi.org/10.1002/mrm.20561 ER - TY - JOUR T1 - Early Immune Response to the Components of the Type III System of Pseudomonas aeruginosa in Children with Cystic Fibrosis AN - 17665566; 6520123 AB - The lungs of patients with cystic fibrosis (CF) are colonized initially by Pseudomonas aeruginosa, which is associated with progressive lung destruction and increased mortality. The pathogenicity of P. aeruginosa is caused by a number of virulence factors, including exotoxin A (ETA) and the type III cytotoxins (ExoS, ExoT, ExoU, and ExoY). P. aeruginosa contacts the plasma membrane to deliver type III cytotoxins through a channel formed by PopB, PopD, and PcrV; ETA enters mammalian cells via receptor-mediated endocytosis. The Wisconsin CF Neonatal Screening Project is a longitudinal investigation to assess the potential benefits and risks of newborn screening for CF; the project was the source of serum samples used in this study. Past studies evaluated the longitudinal appearance of antibodies to ETA and elastase and P. aeruginosa infections in patients with CF. The current study characterized the longitudinal appearance of antibodies to components of the type III system in children with CF. Western blot analyses showed that serum antibodies to PopB, PcrV, and ExoS were common. Longitudinal enzyme-linked immunosorbent assays determined that the first detection of antibodies to pooled ExoS/PopB occurred at a time similar to those of detection of antibodies to a P. aeruginosa cell lysate and the identification of oropharyngeal cultures positive for P. aeruginosa. This indicates that children with CF are colonized early with P. aeruginosa expressing the type III system, implicating it in early pathogenesis, and implies that surveillance of clinical symptoms, oropharyngeal cultures, and seroconversion to type III antigens may facilitate early detection of P. aeruginosa infections. JF - Journal of Clinical Microbiology AU - Corech, R AU - Rao, A AU - Laxova, A AU - Moss, J AU - Rock, MJ AU - Li, Z AU - Kosorok, M R AU - Splaingard, M L AU - Farrell, P M AU - Barbieri, J T AD - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin. Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin. Department of Pediatrics, University of Wisconsin Medical School, Madison, Wisconsin. Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland. Department of Biostatistics and Medical Informatics, University of Wisconsin Medical School, Madison, Wisconsin Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3956 EP - 3962 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 8 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17665566?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Behavioral+Development&rft.atitle=Bidirectional+relations+between+temperament+and+parenting+styles+in+chinese+children&rft.au=Lee%2C+Erica+H.%3BZhou%2C+Qing%3BEisenberg%2C+Nancy%3BWang%2C+Yun&rft.aulast=Lee&rft.aufirst=Erica&rft.date=2013-01-01&rft.volume=37&rft.issue=1&rft.spage=57&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Behavioral+Development&rft.issn=01650254&rft_id=info:doi/10.1177%2F0165025412460795 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Phylogenetic Analysis of the Spirochetes Borrelia parkeri and Borrelia turicatae and the Potential for Tick-Borne Relapsing Fever in Florida AN - 17663669; 6520109 AB - Isolates of Borrelia turicatae, Borrelia parkeri, and the Florida canine borrelia (FCB) were examined to further phylogenetically characterize the identities of these spirochetes in the United States. DNA sequences of four chromosomal loci (the 16S rRNA gene, flaB, gyrB, and glpQ) were determined for eight isolates of B. turicatae and six isolates of B. parkeri, which grouped the spirochetes into two distinct but closely related taxa (>98% sequence identity) separate from Borrelia hermsii. The FCB was clearly separated with the group identified as B. turicatae, confirming this bacterium as a relapsing fever spirochete. Therefore, the potential for tick-borne relapsing fever in humans and other animals exists in Florida and future efforts are needed to determine the enzootic hosts and distribution of this spirochete in the southeastern United States. Analysis of plasmids demonstrated both linear and circular forms in B. turicatae but only linear plasmids in B. parkeri, which should be of interest to investigators concerned with plasmid diversity and evolution within this group of spirochetes. JF - Journal of Clinical Microbiology AU - Schwan, Tom G AU - Raffel, Sandra J AU - Schrumpf, Merry E AU - Policastro, Paul F AU - Rawlings, Julie A AU - Lane, Robert S AU - Breitschwerdt, Edward B AU - Porcella, Stephen F AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana. Community Preparedness Section, Texas Department of State Health Services, Austin, Texas. Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California. Department of Companion and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3851 EP - 3859 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 8 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17663669?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Phylogenetic+Analysis+of+the+Spirochetes+Borrelia+parkeri+and+Borrelia+turicatae+and+the+Potential+for+Tick-Borne+Relapsing+Fever+in+Florida&rft.au=Schwan%2C+Tom+G%3BRaffel%2C+Sandra+J%3BSchrumpf%2C+Merry+E%3BPolicastro%2C+Paul+F%3BRawlings%2C+Julie+A%3BLane%2C+Robert+S%3BBreitschwerdt%2C+Edward+B%3BPorcella%2C+Stephen+F&rft.aulast=Schwan&rft.aufirst=Tom&rft.date=2005-08-01&rft.volume=43&rft.issue=8&rft.spage=3851&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - A chemotactic response facilitates mosquito salivary gland infection by malaria sporozoites AN - 17656550; 6518450 AB - Sporozoite invasion of mosquito salivary glands is critical for malaria transmission to vertebrate hosts. After release into the mosquito hemocoel, the means by which malaria sporozoites locate the salivary glands is unknown. We developed a Matrigel-based in vitro system to observe and analyze the motility of GFP-expressing Plasmodium berghei sporozoites in the presence of salivary gland products of Anopheles stephensi mosquitoes using temperature-controlled, low-light-level video microscopy. Sporozoites moved toward unheated salivary gland homogenate (SGH) but not to SGH that had been heated at 56 degree C for 30 min. We also investigated the origin of the attracted population. Attraction to SGH was restricted to hemolymph- and oocyst-derived sporozoites; salivary gland-derived sporozoites were not attracted to SGH. These data imply that sporozoites employ a chemotactic response to high molecular mass proteins or carbohydrate-binding proteins to locate salivary glands. This raises the possibility of utilizing anti-chemotactic factors for the development of mosquito transmission blocking agents. JF - Journal of Experimental Biology AU - Akaki, Mayumi AU - Dvorak, James A AD - Biochemical and Biophysical Parasitology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Bethesda, MD 20892-8132, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 3211 EP - 3218 PB - Company of Biologists, 140 Cowley Road Cambridge CB4 0DL UK, [URL:http://www.biologists.com/web/index.html] VL - 208 IS - 16 SN - 0022-0949, 0022-0949 KW - Mosquitoes KW - Salivary gland homogenates KW - Sporozoites KW - ASFA 1: Biological Sciences & Living Resources; ASFA 3: Aquatic Pollution & Environmental Quality; Entomology Abstracts KW - Q5 01524:Public health, medicines, dangerous organisms KW - Q1 01306:Physiology, biochemistry, biophysics KW - Z 05206:Medical & veterinary entomology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17656550?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Experimental+Biology&rft.atitle=A+chemotactic+response+facilitates+mosquito+salivary+gland+infection+by+malaria+sporozoites&rft.au=Akaki%2C+Mayumi%3BDvorak%2C+James+A&rft.aulast=Akaki&rft.aufirst=Mayumi&rft.date=2005-08-01&rft.volume=208&rft.issue=16&rft.spage=3211&rft.isbn=&rft.btitle=&rft.title=Journal+of+Experimental+Biology&rft.issn=00220949&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Metallothionein-I/II Double Knockout Mice Are No More Sensitive to the Carcinogenic Effects of Nickel Subsulfide than Wild-Type Mice AN - 17652463; 6486968 AB - Metallothionein (MT) is a high-affinity metal-binding protein thought to mitigate the toxicity of various metals. MT may limit the toxicity of a metal by direct binding or through action as an antioxidant for metals that generate reactive oxygen species. Nickel compounds have carcinogenic potential in humans and animals, possibly by production of oxidative stress. The impact of MT deficiency on the carcinogenic effects of nickel is unknown. Thus, groups (n = 25) of male MT-I/II double knockout (MT-null) or MT wild-type (WT) mice were exposed to a single treatment of nickel (0.5 or 1.0 mg Ni sub(3)S sub(2)/site, intramuscularly, [i.m.], into both hind legs), or left untreated (control) and observed over the next 104 weeks. There were no differences in the incidence of spontaneous tumors in MT-null and WT mice. Nickel induced injection site fibrosarcomas in a dose-related fashion to a similar extent in both WT and MT-null mice. Nickel-treatment had no effect on total lung tumor incidence, although some phenotypic-specific differences occurred in the proportion of benign and malignant pulmonary tumors. Overall, MT-null mice appear no more sensitive to the carcinogenic effects of nickel than WT mice. Thus, poor MT production does not appear to be a predisposing factor for nickel carcinogenesis. JF - International Journal of Toxicology AU - Waalkes, M P AU - Liu, J AU - Kasprzak, K S AU - Diwan, BA AD - Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at the National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 215 EP - 220 VL - 24 IS - 4 SN - 1091-5818, 1091-5818 KW - Toxicology Abstracts KW - X 24164:Pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17652463?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Toxicology&rft.atitle=Metallothionein-I%2FII+Double+Knockout+Mice+Are+No+More+Sensitive+to+the+Carcinogenic+Effects+of+Nickel+Subsulfide+than+Wild-Type+Mice&rft.au=Waalkes%2C+M+P%3BLiu%2C+J%3BKasprzak%2C+K+S%3BDiwan%2C+BA&rft.aulast=Waalkes&rft.aufirst=M&rft.date=2005-08-01&rft.volume=24&rft.issue=4&rft.spage=215&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Toxicology&rft.issn=10915818&rft_id=info:doi/10.1080%2F10915810591000668 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1080/10915810591000668 ER - TY - JOUR T1 - Recommendations for the Design and Use of Standard Virus Panels To Assess Neutralizing Antibody Responses Elicited by Candidate Human Immunodeficiency Virus Type 1 Vaccines AN - 17649752; 6477060 JF - Journal of Virology AU - Mascola, John R AU - D'Souza, Patricia AU - Gilbert, Peter AU - Hahn, Beatrice H AU - Haigwood, Nancy L AU - Morris, Lynn AU - Petropoulos, Christos J AU - Polonis, Victoria R AU - Sarzotti, Marcella AU - Montefiori, David C AD - Vaccine Research Center. Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 10103 EP - 10107 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 16 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts KW - V 22002:AIDS: Molecular and in vitro aspects KW - W3 33240:Immunology KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17649752?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Recommendations+for+the+Design+and+Use+of+Standard+Virus+Panels+To+Assess+Neutralizing+Antibody+Responses+Elicited+by+Candidate+Human+Immunodeficiency+Virus+Type+1+Vaccines&rft.au=Mascola%2C+John+R%3BD%27Souza%2C+Patricia%3BGilbert%2C+Peter%3BHahn%2C+Beatrice+H%3BHaigwood%2C+Nancy+L%3BMorris%2C+Lynn%3BPetropoulos%2C+Christos+J%3BPolonis%2C+Victoria+R%3BSarzotti%2C+Marcella%3BMontefiori%2C+David+C&rft.aulast=Mascola&rft.aufirst=John&rft.date=2005-08-01&rft.volume=79&rft.issue=16&rft.spage=10103&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Specificity of Legionella pneumophila and Coxiella burnetii Vacuoles and Versatility of Legionella pneumophila Revealed by Coinfection AN - 17642171; 6475985 AB - Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niches, African green monkey kidney epithelial (Vero) cells, A/J mouse bone marrow-derived macrophages, human macrophages, and human dendritic cells (DC) containing mature C. burnetii replication vacuoles were superinfected with L. pneumophila, and then the acidity, lysosome-associated membrane protein (LAMP) content, and cohabitation of mature replication vacuoles was assessed. In all cell types, wild-type L. pneumophila occupied distinct vacuoles in close association with acidic, LAMP-positive C. burnetii replication vacuoles. In murine macrophages, but not primate macrophages, DC, or epithelial cells, L. pneumophila replication vacuoles were acidic and LAMP positive. Unlike wild-type L. pneumophila, type IV secretion-deficient dotA mutants trafficked to lysosome-like C. burnetii vacuoles in Vero cells where they survived but failed to replicate. In primate macrophages, DC, or epithelial cells, growth of L. pneumophila was as robust in superinfected cell cultures as in those singly infected. Thus, despite their noted similarities, L. pneumophila and C. burnetii are exquisitely adapted for replication in unique replication vacuoles, and factors that maintain the C. burnetii replication vacuole do not alter biogenesis of an adjacent L. pneumophila replication vacuole. Moreover, L. pneumophila can replicate efficiently in either lysosomal vacuoles of A/J mouse cells or in nonlysosomal vacuoles of primate cells. JF - Infection and Immunity AU - Sauer, John-Demian AU - Shannon, Jeffrey G AU - Howe, Dale AU - Hayes, Stanley F AU - Swanson, Michele S AU - Heinzen, Robert A AD - Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S. 4th St., Hamilton, Montana 59840. Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4494 EP - 4504 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - N 14025:RNA/DNA role in infection & immune response KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17642171?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Parenting%3A+Science+and+Practice&rft.atitle=The+Bio-Culture+of+Parenting%3A+Evidence+From+Five+Cultural+Communities&rft.au=Keller%2C+Heidi%3BLohaus%2C+Arnold%3BKuensemueller%2C+Petra%3BAbels%2C+Monika%3BYovsi%2C+Relindis%3BVoelker%2C+Susanne%3BJensen%2C+Henning%3BPapaligoura%2C+Zaira%3BRosabal-Coto%2C+Mariano%3BKulks%2C+Daniela%3BMohita%2C+Preana&rft.aulast=Keller&rft.aufirst=Heidi&rft.date=2004-01-01&rft.volume=4&rft.issue=1&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Parenting%3A+Science+and+Practice&rft.issn=15295192&rft_id=info:doi/10.1207%2Fs15327922par0401_2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Degradation of Chlamydia pneumoniae by Peripheral Blood Monocytic Cells AN - 17635290; 6475992 AB - Chlamydia pneumoniae is a common human respiratory pathogen that has been associated with a variety of chronic diseases, including atherosclerosis. The role of this organism in the pathogenesis of atherosclerosis remains unknown. A key question is how C. pneumoniae is transferred from the site of primary infection to a developing atherosclerotic plaque. It has been suggested that circulating monocytes could be vehicles for dissemination of C. pneumoniae since the organism has been detected in peripheral blood monocytic cells (PBMCs). In this study we focused on survival of C. pneumoniae within PBMCs isolated from the blood of healthy human donors. We found that C. pneumoniae does not grow and multiply in cultured primary monocytes. In C. pneumoniae-infected monocyte-derived macrophages, growth of the organism was very limited, and the majority of the bacteria were eradicated. We also found that the destruction of C. pneumoniae within infected macrophages resulted in a gradual diminution of chlamydial antigens, although some of these antigens could be detected for days after the initial infection. The detected antigens present in infected monocytes and monocyte-derived macrophages represented neither chlamydial inclusions nor intact organisms. The use of {N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]}-6-aminocaproyl-D-erythro- s phingosine as a vital stain for chlamydiae proved to be a sensitive method for identifying rare C. pneumoniae inclusions and was useful in the detection of even aberrant developmental forms. JF - Infection and Immunity AU - Wolf, Katerina AU - Fischer, Elizabeth AU - Hackstadt, Ted AD - Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, Montana 59840 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4560 EP - 4570 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17635290?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Degradation+of+Chlamydia+pneumoniae+by+Peripheral+Blood+Monocytic+Cells&rft.au=Wolf%2C+Katerina%3BFischer%2C+Elizabeth%3BHackstadt%2C+Ted&rft.aulast=Wolf&rft.aufirst=Katerina&rft.date=2005-08-01&rft.volume=73&rft.issue=8&rft.spage=4560&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - High-Throughput Screening of 11 beta -Hydroxysteroid Dehydrogenase Type 1 in Scintillation Proximity Assay Format AN - 17431044; 6538052 AB - 11 beta -Hydroxysteroid dehydrogenase type-1 (11 beta -HSD1) is a potential target for the treatment of diabetes, obesity, and hyperlipidemia. This enzyme is mainly responsible for reactivating glucocorticoid hormone inside cells such as adipose cells and liver cells by converting the inactive cortisone to active cortisol. Enzyme assays for 11 beta -HSD1 involve either a thin-layer chromatography or high-performance liquid chromatography step to separate cortisol from the substrate cortisone. This additional step is labor intensive and increases the assay time, which limits assay throughput. A homogenous scintillation proximity assay-based method has been recently developed that enables high-throughput screening of 11 beta -HSD1 inhibitors. We have applied this novel 11 beta -HSD1 assay to screening a large-size compound collection and identified several structural classes of lead compounds that selectively inhibit the activity of 11 beta -HSD1. JF - Assay and Drug Development Technologies AU - Solly, K AU - Mundt, S S AU - Zokian, HJ AU - Ding, GJ-F AU - Hermanowski-Vosatka, A AU - Strulovici, B AU - Zheng, W AD - National Institutes of Health, National Human Genome Research Institute, NIH Chemical Genomics Center, 9800 Medical Center Drive, MSC: 3370, Bethesda, MD 20892, USA, wzheng@mail.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 377 VL - 3 IS - 4 SN - 1540-658X, 1540-658X KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - High-performance liquid chromatography KW - Obesity KW - Hydrocortisone KW - 11^b-Hydroxysteroid dehydrogenase KW - Hepatocytes KW - Hyperlipidemia KW - Hormones KW - Glucocorticoids KW - Diabetes mellitus KW - Scintillation KW - high-throughput screening KW - Thin-layer chromatography KW - W 30965:Miscellaneous, Reviews KW - W3 33250:Methods: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17431044?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Assay+and+Drug+Development+Technologies&rft.atitle=High-Throughput+Screening+of+11+beta+-Hydroxysteroid+Dehydrogenase+Type+1+in+Scintillation+Proximity+Assay+Format&rft.au=Solly%2C+K%3BMundt%2C+S+S%3BZokian%2C+HJ%3BDing%2C+GJ-F%3BHermanowski-Vosatka%2C+A%3BStrulovici%2C+B%3BZheng%2C+W&rft.aulast=Solly&rft.aufirst=K&rft.date=2005-08-01&rft.volume=3&rft.issue=4&rft.spage=377&rft.isbn=&rft.btitle=&rft.title=Assay+and+Drug+Development+Technologies&rft.issn=1540658X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - 11^b-Hydroxysteroid dehydrogenase; Hydrocortisone; high-throughput screening; Scintillation; High-performance liquid chromatography; Thin-layer chromatography; Glucocorticoids; Obesity; Hormones; Hepatocytes; Diabetes mellitus; Hyperlipidemia ER - TY - JOUR T1 - Dietary Benzo[a]Pyrene Intake and Risk of Colorectal Adenoma AN - 17406020; 6522172 AB - We carried out a clinic-based case-control study specifically designed to address the hypothesis that dietary intake of polycyclic aromatic hydrocarbons (PAH) is associated with colorectal adenoma risk. We developed a food frequency questionnaire with detailed questions on meat-cooking methods and doneness levels and a benzo[a]pyrene (BaP) database (as a surrogate for total carcinogenic PAHs) based on the collection and analysis of a wide range of food samples. We estimated BaP intake derived from meat and from all foods to test its relationship with risk of colorectal adenomas. The median (10th and 90th percentiles) BaP intake in controls was 5 ng/d (0.2 and 66 ng/d) estimated from meat and 73 ng/d (35 and 140 ng/d) from all foods. In cases, median BaP intake was 17 ng/d (0.5 and 101 ng/d) from meat and 76 ng/d (44 and 163 ng/d) from all foods. Multivariate analysis was carried out on 146 cases and 228 controls. The odds ratios (95% confidence interval) for dietary BaP from meat with the first quintile as the reference group were 1.19 (0.51-2.80) for the second quintile, 1.71 (0.76-3.83) for the third quintile, 2.16 (0.96-4.86) for the fourth quintile, and 2.82 (1.24-6.43) for the fifth quintile (P sub(trend) = 0.01). Increased risk of colorectal adenomas was more strongly associated with BaP intake estimated from all foods: 2.61 (1.08-6.29) for the second quintile, 4.21 (1.79-9.91) for the third quintile, 2.45 (0.98-6.12) for the fourth quintile, and 5.60 (2.20-14.20) for the fifth quintile (P sub(trend) = 0.002). This study provides evidence that dietary BaP plays a role in colorectal adenoma etiology. JF - Cancer Epidemiology, Biomarkers & Prevention AU - Sinha, Rashmi AU - Kulldorff, Martin AU - Gunter, Marc J AU - Strickland, Paul AU - Rothman, Nathaniel AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Rockville, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 2030 EP - 2034 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 8 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts KW - Meat KW - Databases KW - Polycyclic aromatic hydrocarbons KW - Multivariate analysis KW - Food KW - Benzo(a)pyrene KW - Adenoma KW - Dietary intake KW - biomarkers KW - X 24190:Polycyclic hydrocarbons UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17406020?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Dietary+Benzo%5Ba%5DPyrene+Intake+and+Risk+of+Colorectal+Adenoma&rft.au=Sinha%2C+Rashmi%3BKulldorff%2C+Martin%3BGunter%2C+Marc+J%3BStrickland%2C+Paul%3BRothman%2C+Nathaniel&rft.aulast=Sinha&rft.aufirst=Rashmi&rft.date=2005-08-01&rft.volume=14&rft.issue=8&rft.spage=2030&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Meat; Databases; Polycyclic aromatic hydrocarbons; Multivariate analysis; Food; Benzo(a)pyrene; biomarkers; Dietary intake; Adenoma ER - TY - JOUR T1 - The agr Radiation: an Early Event in the Evolution of Staphylococci AN - 17402946; 6517740 AB - agr is a global regulatory system in the staphylococci, operating by a classical two-component signaling module and controlling the expression of most of the genes encoding extracellular virulence factors. As it is autoinduced by a peptide, encoded within the locus, that is the ligand for the signal receptor, it is a sensor of population density or a quorum sensor and is the only known quorum-sensing system in the genus. agr is conserved throughout the staphylococci but has diverged along lines that appear to parallel speciation and subspeciation within the genus. This divergence has given rise to a novel type of interstrain and interspecies cross-inhibition that represents a fundamental aspect of the organism's biology and may be a predominant feature of the evolutionary forces that have driven it. We present evidence, using a newly developed, luciferase-based agr typing scheme, that the evolutionary divergence of the agr system was an early event in the evolution of the staphylococci and long preceded the development of the nucleotide polymorphisms presently used for genotyping. These polymorphisms developed, for the most part, within different agr groups; mobile genetic elements appear also to have diffused recently and, with a few notable exceptions, have come to reside largely indiscriminately within the several agr groups. JF - Journal of Bacteriology AU - Wright, Jesse SIII AU - Traber, Katrina E AU - Corrigan, Rebecca AU - Benson, Sarah A AU - Musser, James M AU - Novick, Richard P AD - Molecular Pathogenesis Program and Department of Microbiology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, New York 10016. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840. Center for Human Bacterial Pathogenesis Research, Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5585 EP - 5594 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Speciation KW - virulence factors KW - Gene polymorphism KW - Genotyping KW - Staphylococcus KW - Population density KW - Evolution KW - Nucleotides KW - Signal transduction KW - G 07320:Bacterial genetics KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17402946?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=The+agr+Radiation%3A+an+Early+Event+in+the+Evolution+of+Staphylococci&rft.au=Wright%2C+Jesse+SIII%3BTraber%2C+Katrina+E%3BCorrigan%2C+Rebecca%3BBenson%2C+Sarah+A%3BMusser%2C+James+M%3BNovick%2C+Richard+P&rft.aulast=Wright&rft.aufirst=Jesse&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5585&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Speciation; virulence factors; Genotyping; Gene polymorphism; Population density; Nucleotides; Evolution; Signal transduction; Staphylococcus ER - TY - JOUR T1 - Cellular Mechanisms for Low-Dose Ionizing Radiation-Induced Perturbation of the Breast Tissue Microenvironment AN - 17399440; 6517116 AB - Radiation exposure is an important form of environmental carcinogen and has been associated with increased risk of breast cancer. Epigenetic events, especially those involving alterations in the breast stromal microenvironment, may play an important role in radiation-induced carcinogenesis but remain not well understood. We here show that human mammary stromal fibroblasts respond to protracted low-dose ionizing radiation exposures by displaying a senescence-like phenotype. Using a three-dimensional coculture system to model the interactions of different mammary cell types with their neighbors and with their environment, we provide a direct experimental proof that ionizing radiation-induced senescence-like fibroblasts significantly perturb the mammary stromal microenvironment, which is highlighted by impaired formation of pseudopodia networks due to marked cytoskeletal alterations in senescence-like fibroblasts and increased extracellular matrix degradation because of the up-regulation of multiple secreted matrix metalloproteinases. Within such a perturbed environment, mammary ductal morphogenesis is completely disrupted and epithelial cells instead grow into enlarged cystic structures, which further develop and become disorganized cell masses on inactivation of cellular death pathways. Breast carcinoma cells growing in such an environment are enabled to fully express their malignant potential as evidenced by the alpha 6 beta 4 integrin/phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin pathway-dependent invasive growth. Our results suggest that ionizing radiation, in addition to causing gene mutations in epithelial cells, can contribute to breast carcinogenesis by perturbing the tissue microenvironment that leads to dysregulated cell-cell and cell-matrix interactions. JF - Cancer Research AU - Tsai, Kelvin KC AU - Chuang, Eric Yao-Yu AU - Little, John B AU - Yuan, Zhi-Min AD - Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts and Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 6734 EP - 6744 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 15 SN - 0008-5472, 0008-5472 KW - Toxicology Abstracts KW - Epithelial cells KW - X radiation KW - Matrix metalloproteinase KW - Carcinogens KW - Fibroblasts KW - Cytoskeleton KW - 1-Phosphatidylinositol 3-kinase KW - Ionizing radiation KW - Carcinogenesis KW - AKT protein KW - Microenvironments KW - Breast carcinoma KW - X 24210:Radiation & radioactive materials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17399440?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=Cellular+Mechanisms+for+Low-Dose+Ionizing+Radiation-Induced+Perturbation+of+the+Breast+Tissue+Microenvironment&rft.au=Tsai%2C+Kelvin+KC%3BChuang%2C+Eric+Yao-Yu%3BLittle%2C+John+B%3BYuan%2C+Zhi-Min&rft.aulast=Tsai&rft.aufirst=Kelvin&rft.date=2005-08-01&rft.volume=65&rft.issue=15&rft.spage=6734&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Cytoskeleton; Epithelial cells; 1-Phosphatidylinositol 3-kinase; X radiation; Ionizing radiation; Carcinogenesis; AKT protein; Matrix metalloproteinase; Microenvironments; Breast carcinoma; Carcinogens; Fibroblasts ER - TY - JOUR T1 - The Bacteriophage P1 hot Gene Product Can Substitute for the Escherichia coli DNA Polymerase III theta Subunit AN - 17397837; 6517734 AB - The theta subunit (holE gene product) of Escherichia coli DNA polymerase (Pol) III holoenzyme is a tightly bound component of the polymerase core. Within the core ( alpha - epsilon - theta ), the alpha and epsilon subunits carry the DNA polymerase and 3' proofreading functions, respectively, while the precise function of theta is unclear. holE homologs are present in genomes of other enterobacteriae, suggestive of a conserved function. Putative homologs have also been found in the genomes of bacteriophage P1 and of certain conjugative plasmids. The presence of these homologs is of interest, because these genomes are fully dependent on the host replication machinery and contribute few, if any, replication factors themselves. To study the role of these theta homologs, we have constructed an E. coli strain in which holE is replaced by the P1 homolog, hot. We show that hot is capable of substituting for holE when it is assayed for its antimutagenic action on the proofreading-impaired dnaQ49 mutator, which carries a temperature-sensitive epsilon subunit. The ability of hot to substitute for holE was also observed with other, although not all, dnaQ mutator alleles tested. The data suggest that the P1 hot gene product can substitute for the theta subunit and is likely incorporated in the Pol III complex. We also show that overexpression of either theta or Hot further suppresses the dnaQ49 mutator phenotype. This suggests that the complexing of dnaQ49- epsilon with theta is rate limiting for its ability to proofread DNA replication errors. The possible role of hot for bacteriophage P1 is discussed. JF - Journal of Bacteriology AU - Chikova, Anna K AU - Schaaper, Roel M AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709. D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Science, Moscow, 123098, Russia Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 5528 EP - 5536 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 16 SN - 0021-9193, 0021-9193 KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts; Genetics Abstracts KW - Genomes KW - Phages KW - DNA biosynthesis KW - Data processing KW - Replication KW - DNA-directed DNA polymerase KW - Escherichia coli KW - Plasmids KW - Proofreading KW - J 02725:DNA KW - G 07320:Bacterial genetics KW - N 14820:DNA Metabolism & Structure KW - V 22070:Phage-host interactions including lysogeny & transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17397837?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=The+Bacteriophage+P1+hot+Gene+Product+Can+Substitute+for+the+Escherichia+coli+DNA+Polymerase+III+theta+Subunit&rft.au=Chikova%2C+Anna+K%3BSchaaper%2C+Roel+M&rft.aulast=Chikova&rft.aufirst=Anna&rft.date=2005-08-01&rft.volume=187&rft.issue=16&rft.spage=5528&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Phages; Genomes; DNA biosynthesis; Data processing; Replication; DNA-directed DNA polymerase; Plasmids; Proofreading; Escherichia coli ER - TY - JOUR T1 - Biological dust exposure in the workplace is a risk factor for chronic obstructive pulmonary disease AN - 17397768; 6519789 AB - BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity and mortality. Although the main risk factor is smoking, 15-19% of COPD even in smokers has been attributed to occupational exposures. The aim of this study was to investigate the association between occupational exposure and risk of COPD. METHODS: Participants were part of a cross sectional study of risk factors for COPD. A total of 1232 completed a detailed respiratory questionnaire, spirometric testsing and measurement of gas transfer. Job histories were coded according to the International Standard Classification of Occupations. These codes were then used to establish occupational exposures using the ALOHA job exposure matrix. RESULTS: The prevalence of emphysema was 2.4%, chronic obstructive bronchitis 1.8%, and COPD 3.4%. Subjects ever exposed to biological dusts had an increased risk of chronic obstructive bronchitis (OR 3.19; 95% CI 1.27 to 7.97), emphysema (OR 3.18; 95% CI 1.41 to 7.13), and COPD (OR 2.70, 95% CI 1.39 to 5.23). These risks were higher in women than in men. For biological dust, the risk of emphysema and COPD was also significantly increased in both the duration of exposure categories, again in women but not in men. No significant increased risks for COPD were found for mineral dust (OR 1.13; 95% CI 0.57 to 2.27) or gases/fumes (OR 1.63; 95% CI 0.83 to 3.22). CONCLUSION: In this general population sample of adults, occupational exposures to biological dusts were associated with an increased risk of COPD which was higher in women. Preventive strategies should be aimed at reducing exposure to these agents in the workplace. JF - Thorax AU - Matheson, M C AU - Benke, G AU - Raven, J AU - Sim, M R AU - Kromhout, H AU - Vermeulen, R AU - Johns, D P AU - Walters, E H AU - Abramson, M J AD - Department of Epidemiology and Preventive Medicine, Monash University, Victoria, Australia. Department of Allergy, Immunology and Respiratory Medicine, The Alfred Hospital, Victoria, Australia. Division of Occupational and Environmental Health, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands. Occupational and Environmental Epidemiology Branch, National Cancer Institute, Rockville, MD 20852-7240, USA. Cardio-Respiratory Research Group, School of Medicine, University of Tasmania, Hobart, Australia Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 645 EP - 651 PB - B M J Publishing Group, B.M.A. House Tavistock Sq. London WC1H 9JR UK VL - 60 IS - 8 SN - 0040-6376, 0040-6376 KW - Health & Safety Science Abstracts; Toxicology Abstracts; Immunology Abstracts KW - Historical account KW - Morbidity KW - Dust KW - Smoking KW - Classification KW - Risk factors KW - Bronchitis KW - Occupational exposure KW - chronic obstructive pulmonary disease KW - International standardization KW - Mortality KW - Emphysema KW - Fumes KW - Obstructive lung disease KW - International standards KW - Gases KW - classification KW - Minerals KW - X 24240:Miscellaneous KW - F 06364:Respiratory System: Animal KW - H 1000:Occupational Safety and Health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17397768?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Thorax&rft.atitle=Biological+dust+exposure+in+the+workplace+is+a+risk+factor+for+chronic+obstructive+pulmonary+disease&rft.au=Matheson%2C+M+C%3BBenke%2C+G%3BRaven%2C+J%3BSim%2C+M+R%3BKromhout%2C+H%3BVermeulen%2C+R%3BJohns%2C+D+P%3BWalters%2C+E+H%3BAbramson%2C+M+J&rft.aulast=Matheson&rft.aufirst=M&rft.date=2005-08-01&rft.volume=60&rft.issue=8&rft.spage=645&rft.isbn=&rft.btitle=&rft.title=Thorax&rft.issn=00406376&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Emphysema; Mortality; Fumes; Obstructive lung disease; Dust; Morbidity; International standards; Smoking; Gases; Classification; Risk factors; Bronchitis; Minerals; Occupational exposure; Historical account; classification; International standardization; chronic obstructive pulmonary disease ER - TY - JOUR T1 - Prevention and Treatment of Cutaneous Leishmaniasis in Primates by Using Synthetic Type D/A Oligodeoxynucleotides Expressing CpG Motifs AN - 17384845; 6476034 AB - Oligodeoxynucleotides (ODN) containing CpG motifs mimic microbial DNA and are recognized by toll-like receptor 9 on immune cells. The resulting response limits the early spread of infectious organisms and promotes the development of adaptive immunity. In this regard, CpG ODN show promise as immunoprotective agents and as vaccine adjuvants. Previous studies of nonhuman primates showed that administration of CpG ODN type D (also known as type A) at the site of infection 3 days before and after a challenge with Leishmania major enhanced host resistance and reduced the lesion's severity. In this study, we show that systemic administration of D/A ODN limits the size of lesions following an intradermal infection with L. major. Importantly, the reduced morbidity was not associated with a reduction in long-term immunity, as such treated macaques were still protected following a secondary challenge. Finally, administration of D/A ODN to macaques that had established cutaneous lesions reduced the severity of the lesions, suggesting a potential role for CpG ODN in L. major treatment. Together, these findings support the development of clinical studies to assess the use of CpG ODN types D/A as immunoprotective and therapeutic agents. JF - Infection and Immunity AU - Flynn, Barbara AU - Wang, Vivian AU - Sacks, David L AU - Seder, Robert A AU - Verthelyi, Daniela AD - Division of Therapeutic Proteins, Center for Drug Evaluation and Review, Food and Drug Administration, Washington, D.C. Vaccine Research Center. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 4948 EP - 4954 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 8 SN - 0019-9567, 0019-9567 KW - Primates KW - Macaques KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology; Biochemistry Abstracts 2: Nucleic Acids KW - Adjuvants KW - Infection KW - Oligonucleotides KW - Morbidity KW - Leishmania major KW - Macaca KW - Immunity KW - CpG islands KW - Vaccines KW - Toll-like receptors KW - Cutaneous leishmaniasis KW - K 03086:Immunology & vaccination KW - N 14025:RNA/DNA role in infection & immune response KW - F 06100:Vaccines - active immunity KW - W3 33345:DNA vaccines KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17384845?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Prevention+and+Treatment+of+Cutaneous+Leishmaniasis+in+Primates+by+Using+Synthetic+Type+D%2FA+Oligodeoxynucleotides+Expressing+CpG+Motifs&rft.au=Flynn%2C+Barbara%3BWang%2C+Vivian%3BSacks%2C+David+L%3BSeder%2C+Robert+A%3BVerthelyi%2C+Daniela&rft.aulast=Flynn&rft.aufirst=Barbara&rft.date=2005-08-01&rft.volume=73&rft.issue=8&rft.spage=4948&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Primates; Macaca; Leishmania major; CpG islands; Oligonucleotides; Immunity; Infection; Morbidity; Vaccines; Adjuvants; Toll-like receptors; Cutaneous leishmaniasis ER - TY - JOUR T1 - Replicating Rather than Nonreplicating Adenovirus-Human Immunodeficiency Virus Recombinant Vaccines Are Better at Eliciting Potent Cellular Immunity and Priming High-Titer Antibodies AN - 17378478; 6477070 AB - A major challenge in combating the human immunodeficiency virus (HIV) epidemic is the development of vaccines capable of inducing potent, persistent cellular immunity and broadly reactive neutralizing antibody responses to HIV type 1 (HIV-1). We report here the results of a preclinical trial using the chimpanzee model to investigate a combination vaccine strategy involving sequential priming immunizations with different serotypes of adenovirus (Ad)/HIV-1 sub(MN)env/rev recombinants and boosting with an HIV envelope subunit protein, oligomeric HIV sub(SF162) gp140 Delta V2. The immunogenicities of replicating and nonreplicating Ad/HIV-1 sub(MN)env/rev recombinants were compared. Replicating Ad/HIV recombinants were better at eliciting HIV-specific cellular immune responses and better at priming humoral immunity against HIV than nonreplicating Ad-HIV recombinants carrying the same gene insert. Enhanced cellular immunity was manifested by a greater frequency of HIV envelope-specific gamma interferon-secreting peripheral blood lymphocytes and better priming of T-cell proliferative responses. Enhanced humoral immunity was seen in higher anti-envelope binding and neutralizing antibody titers and better induction of antibody-dependent cellular cytotoxicity. More animals primed with replicating Ad recombinants mounted neutralizing antibodies against heterologous R5 viruses after one or two booster immunizations with the mismatched oligomeric HIV-1 sub(SF162) gp140 Delta V2 protein. These results support continued development of the replicating Ad-HIV recombinant vaccine approach and suggest that the use of replicating vectors for other vaccines may prove fruitful. JF - Journal of Virology AU - Peng, Bo AU - Wang, Liqun Rejean AU - Gomez-Roman, Victor Raul AU - Davis-Warren, Alberta AU - Montefiori, David C AU - Kalyanaraman, V S AU - Venzon, David AU - Zhao, Jun AU - Kan, Elaine AU - Rowell, Thomas J AU - Murthy, Krishna K AU - Srivastava, Indresh AU - Barnett, Susan W AU - Robert-Guroff, Marjorie AD - Vaccine Branch. Biostatistics and Data Management Section, National Cancer Institute, Bethesda, Maryland 20892. Department of Surgery, Laboratory for AIDS Vaccine Research & Development, Duke University Medical Center, Durham, North Carolina 27710. Advanced BioScience Laboratories, Inc., Kensington, Maryland 20895. Chiron Corp., Emeryville, California 94608-2916. University of Louisiana at Lafayette, New Iberia Research Center, New Iberia, Louisiana 70560. Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227 Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 10200 EP - 10209 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 16 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Genetics Abstracts; Immunology Abstracts; Virology & AIDS Abstracts KW - gamma -Interferon KW - Serotypes KW - Epidemics KW - Adenovirus KW - Peripheral blood KW - Lymphocytes KW - Clinical trials KW - Immunization KW - Immunity (humoral) KW - Antibodies KW - Cytotoxicity KW - Envelopes KW - Immunity (cell-mediated) KW - Immunogenicity KW - Human immunodeficiency virus 1 KW - Envelope protein KW - Lymphocytes T KW - Vaccines KW - W3 33365:Vaccines (other) KW - G 07240:Immunogenetics KW - G 07313:Viruses KW - F 06100:Vaccines - active immunity KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17378478?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Replicating+Rather+than+Nonreplicating+Adenovirus-Human+Immunodeficiency+Virus+Recombinant+Vaccines+Are+Better+at+Eliciting+Potent+Cellular+Immunity+and+Priming+High-Titer+Antibodies&rft.au=Peng%2C+Bo%3BWang%2C+Liqun+Rejean%3BGomez-Roman%2C+Victor+Raul%3BDavis-Warren%2C+Alberta%3BMontefiori%2C+David+C%3BKalyanaraman%2C+V+S%3BVenzon%2C+David%3BZhao%2C+Jun%3BKan%2C+Elaine%3BRowell%2C+Thomas+J%3BMurthy%2C+Krishna+K%3BSrivastava%2C+Indresh%3BBarnett%2C+Susan+W%3BRobert-Guroff%2C+Marjorie&rft.aulast=Peng&rft.aufirst=Bo&rft.date=2005-08-01&rft.volume=79&rft.issue=16&rft.spage=10200&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - gamma -Interferon; Epidemics; Serotypes; Peripheral blood; Lymphocytes; Clinical trials; Immunization; Immunity (humoral); Cytotoxicity; Antibodies; Envelopes; Immunity (cell-mediated); Immunogenicity; Envelope protein; Lymphocytes T; Vaccines; Human immunodeficiency virus 1; Adenovirus ER - TY - JOUR T1 - PAMAM Dendrimer Based Macromolecules as Improved Contrast Agents AN - 17360432; 6436873 AB - Dendrimers are an attractive platform for macromolecular imaging due to the presence of multiple terminal groups on the exterior of the molecule. Through application of appropriate metal ion chelates, large numbers of metal ions for imaging (paramagnetic or radioopaque) and therapy (radioactive particle emitters) may be conjugated to the dendrimer in combination with a targeting vector, through classic organic synthetic techniques. Thus, a large amount of these metal ions potentially may be site specifically delivered directly into the body with the dendrimer as the vehicle with the targeting vector directing the modified dendrimer. The development of targeted macromolecular agents with acceptable blood retention times and selective organ uptake then has the potential for various biological applications. A review of comparative studies of dendrimers with various externally appended imaging and targeting agents is presented herein. JF - Molecular Pharmaceutics AU - Venditto, V J AU - Regino, CAS AU - Brechbiel, M W AD - Radioimmune & Inorganic Chemistry Section, Radiation Oncology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA Y1 - 2005/08/01/ PY - 2005 DA - 2005 Aug 01 SP - 302 EP - 311 VL - 2 IS - 4 SN - 1543-8384, 1543-8384 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Metals KW - Ions KW - Blood KW - Macromolecules KW - Reviews KW - Contrast media KW - Chelates KW - imaging KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17360432?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Pharmaceutics&rft.atitle=PAMAM+Dendrimer+Based+Macromolecules+as+Improved+Contrast+Agents&rft.au=Venditto%2C+V+J%3BRegino%2C+CAS%3BBrechbiel%2C+M+W&rft.aulast=Venditto&rft.aufirst=V&rft.date=2005-08-01&rft.volume=2&rft.issue=4&rft.spage=302&rft.isbn=&rft.btitle=&rft.title=Molecular+Pharmaceutics&rft.issn=15438384&rft_id=info:doi/10.1021%2Fmp050019e LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Blood; Ions; Metals; Macromolecules; Reviews; Contrast media; Chelates; imaging DO - http://dx.doi.org/10.1021/mp050019e ER - TY - JOUR T1 - Lyme disease agent borrows a practical coat AN - 17353221; 6422760 AB - The bacterium that causes Lyme disease is a manipulative creature. This pathogen exploits a component in the saliva of its vector, a tick, to facilitate invasion of vertebrate hosts. JF - Nature Medicine AU - Rosa, P AD - Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA, prosa@niaid.nih.gov Y1 - 2005/08// PY - 2005 DA - Aug 2005 SP - 831 EP - 832 VL - 11 IS - 8 SN - 1078-8956, 1078-8956 KW - Microbiology Abstracts B: Bacteriology KW - J 02855:Human Bacteriology: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17353221?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Medicine&rft.atitle=Lyme+disease+agent+borrows+a+practical+coat&rft.au=Rosa%2C+P&rft.aulast=Rosa&rft.aufirst=P&rft.date=2005-08-01&rft.volume=11&rft.issue=8&rft.spage=831&rft.isbn=&rft.btitle=&rft.title=Nature+Medicine&rft.issn=10788956&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Smaller head of the hippocampus in Gulf War-related posttraumatic stress disorder. AN - 68001586; 15967648 AB - Reductions in hippocampal volume and impairment in short-term verbal memory have been reported in Vietnam combat veterans with posttraumatic stress disorder (PTSD) and in women with abuse-related PTSD. The present investigation evaluated hippocampal volume and memory in Gulf War veterans. This research is timely given the ongoing war in Iraq and the anticipated high rates of PTSD among returning combat soldiers. Fourteen veterans with PTSD related to traumatic experiences during the Gulf War (1990-1991), 23 deployed veterans without PTSD, 22 non-deployed reservists and 29 healthy civilians were studied. Volumes of the hippocampus, temporal lobe, and whole brain were measured on coronal MRI scans, and hippocampal mediated memory function was evaluated. The head of the hippocampus was the only subregion that was significantly smaller in Gulf War veterans with PTSD than in healthy civilians. Deployed veterans with PTSD, deployed veterans without PTSD, and non-deployed reservists had significantly smaller whole hippocampal volume and lower scores on immediate and delayed verbal and visual retrieval compared with healthy civilians. JF - Psychiatry research AU - Vythilingam, Meena AU - Luckenbaugh, David A AU - Lam, Thomas AU - Morgan, Charles A AU - Lipschitz, Deborah AU - Charney, Dennis S AU - Bremner, J Douglas AU - Southwick, Steven M AD - Mood and Anxiety Disorders Program, National Institute of Mental Health, MAP, 15K North Drive, Room #111, MSC 2670, Bethesda, MD 20892-2670, USA. meena.vythi@nih.gov Y1 - 2005/07/30/ PY - 2005 DA - 2005 Jul 30 SP - 89 EP - 99 VL - 139 IS - 2 SN - 0165-1781, 0165-1781 KW - Index Medicus KW - Temporal Lobe -- anatomy & histology KW - Magnetic Resonance Imaging KW - Cognition Disorders -- diagnosis KW - Cognition Disorders -- epidemiology KW - Humans KW - Adult KW - Military Personnel -- statistics & numerical data KW - Observer Variation KW - Neuropsychological Tests KW - Male KW - Functional Laterality -- physiology KW - Female KW - Temporal Lobe -- physiopathology KW - Stress Disorders, Post-Traumatic -- etiology KW - Hippocampus -- physiopathology KW - Stress Disorders, Post-Traumatic -- physiopathology KW - Persian Gulf Syndrome -- psychology KW - Hippocampus -- anatomy & histology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68001586?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychiatry+research&rft.atitle=Smaller+head+of+the+hippocampus+in+Gulf+War-related+posttraumatic+stress+disorder.&rft.au=Vythilingam%2C+Meena%3BLuckenbaugh%2C+David+A%3BLam%2C+Thomas%3BMorgan%2C+Charles+A%3BLipschitz%2C+Deborah%3BCharney%2C+Dennis+S%3BBremner%2C+J+Douglas%3BSouthwick%2C+Steven+M&rft.aulast=Vythilingam&rft.aufirst=Meena&rft.date=2005-07-30&rft.volume=139&rft.issue=2&rft.spage=89&rft.isbn=&rft.btitle=&rft.title=Psychiatry+research&rft.issn=01651781&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dose escalation trial designs based on a molecularly targeted endpoint. AN - 67988941; 15909289 AB - Traditional phase I dose-finding studies for chemotoxic agents base dose escalation on toxicity, with escalation continuing until unacceptable toxicity is observed. Recent development of molecularly targeted agents that have little or no toxicity in the therapeutic dose range has raised questions over the best study designs for phase I studies. Two types of designs are proposed and evaluated in this paper. In these designs, escalation is based on a binary response that indicates whether or not the agent has had the desired effect on the molecular target. One design is developed to ensure that if the true target response rate is low there will be a high probability of escalating and if the true target response rate is high there will be a low probability of escalating. The other design is developed to continue to escalate as long as the true response rate is increasing and to stop escalating when the response rate plateaus or decreases. A limited simulation study is performed and the designs are compared with respect to the dose level at the end of escalation and the number of patients treated on study. JF - Statistics in medicine AU - Hunsberger, Sally AU - Rubinstein, Lawrence V AU - Dancey, Janet AU - Korn, Edward L AD - Biometrics Research Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. sallyh@ctep.nci.nih.gov Y1 - 2005/07/30/ PY - 2005 DA - 2005 Jul 30 SP - 2171 EP - 2181 VL - 24 IS - 14 SN - 0277-6715, 0277-6715 KW - Index Medicus KW - Computer Simulation KW - Dose-Response Relationship, Drug KW - Humans KW - Maximum Tolerated Dose KW - Research Design KW - Clinical Trials, Phase I as Topic -- methods KW - Drug Evaluation -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67988941?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Statistics+in+medicine&rft.atitle=Dose+escalation+trial+designs+based+on+a+molecularly+targeted+endpoint.&rft.au=Hunsberger%2C+Sally%3BRubinstein%2C+Lawrence+V%3BDancey%2C+Janet%3BKorn%2C+Edward+L&rft.aulast=Hunsberger&rft.aufirst=Sally&rft.date=2005-07-30&rft.volume=24&rft.issue=14&rft.spage=2171&rft.isbn=&rft.btitle=&rft.title=Statistics+in+medicine&rft.issn=02776715&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-20 N1 - Date created - 2005-06-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena. AN - 68070603; 15897201 AB - Nuclease A (NucA) is a nonspecific endonuclease from Anabaena sp. capable of degrading single- and double-stranded DNA and RNA in the presence of divalent metal ions. We have determined the structure of the delta(2-24),D121A mutant of NucA in the presence of Zn2+ and Mn2+ (PDB code 1ZM8). The mutations were introduced to remove the N-terminal signal peptide and to reduce the activity of the nonspecific nuclease, thereby reducing its toxicity to the Escherichia coli expression system. NucA contains a betabeta alpha metal finger motif and a hydrated Mn2+ ion at the active site. Unexpectedly, NucA was found to contain additional metal binding sites approximately 26 A apart from the catalytic metal binding site. A structural comparison between NucA and the closest analog for which structural data exist, the Serratia nuclease, indicates several interesting differences. First, NucA is a monomer rather than a dimer. Second, there is an unexpected structural homology between the N-terminal segments despite a poorly conserved sequence, which in Serratia includes a cysteine bridge thought to play a regulatory role. In addition, although a sequence alignment had suggested that NucA lacks a proposed catalytic residue corresponding to Arg57 in Serratia, the structure determined here indicates that Arg93 in NucA is positioned to fulfill this role. Based on comparison with DNA-bound nuclease structures of the betabeta alpha metal finger nuclease family and available mutational data on NucA, we propose that His124 acts as a catalytic base, and Arg93 participates in the catalysis possibly through stabilization of the transition state. JF - The Journal of biological chemistry AU - Ghosh, Mahua AU - Meiss, Gregor AU - Pingoud, Alfred AU - London, Robert E AU - Pedersen, Lars C AD - Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/07/29/ PY - 2005 DA - 2005 Jul 29 SP - 27990 EP - 27997 VL - 280 IS - 30 SN - 0021-9258, 0021-9258 KW - Cations KW - 0 KW - Disulfides KW - Ions KW - Protein Sorting Signals KW - Recombinant Proteins KW - Manganese KW - 42Z2K6ZL8P KW - Histidine KW - 4QD397987E KW - RNA KW - 63231-63-0 KW - DNA KW - 9007-49-2 KW - Arginine KW - 94ZLA3W45F KW - Endonucleases KW - EC 3.1.- KW - sugar-nonspecific nuclease KW - Zinc KW - J41CSQ7QDS KW - Cysteine KW - K848JZ4886 KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Catalytic Domain KW - Zinc -- chemistry KW - Mutagenesis, Site-Directed KW - Cysteine -- chemistry KW - Amino Acid Motifs KW - Molecular Sequence Data KW - DNA -- chemistry KW - Recombinant Proteins -- chemistry KW - Sequence Homology, Amino Acid KW - Escherichia coli -- metabolism KW - Arginine -- chemistry KW - Models, Molecular KW - Dimerization KW - DNA Mutational Analysis KW - Serratia -- metabolism KW - Amino Acid Sequence KW - Hydrolysis KW - Binding Sites KW - Static Electricity KW - Histidine -- chemistry KW - Manganese -- chemistry KW - Protein Folding KW - Oxygen -- chemistry KW - RNA -- chemistry KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Catalysis KW - Endonucleases -- metabolism KW - Endonucleases -- chemistry KW - Anabaena -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68070603?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Structural+insights+into+the+mechanism+of+nuclease+A%2C+a+betabeta+alpha+metal+nuclease+from+Anabaena.&rft.au=Ghosh%2C+Mahua%3BMeiss%2C+Gregor%3BPingoud%2C+Alfred%3BLondon%2C+Robert+E%3BPedersen%2C+Lars+C&rft.aulast=Ghosh&rft.aufirst=Mahua&rft.date=2005-07-29&rft.volume=280&rft.issue=30&rft.spage=27990&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-07-25 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1ZM8; PDB N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene expression responses to DNA damage are altered in human aging and in Werner Syndrome. AN - 68083263; 15897889 AB - The accumulation of DNA damage and mutations is considered a major cause of cancer and aging. While it is known that DNA damage can affect changes in gene expression, transcriptional regulation after DNA damage is poorly understood. We characterized the expression of 6912 genes in human primary fibroblasts after exposure to three different kinds of cellular stress that introduces DNA damage: 4-nitroquinoline-1-oxide (4NQO), gamma-irradiation, or UV-irradiation. Each type of stress elicited damage specific gene expression changes of up to 10-fold. A total of 85 genes had similar changes in expression of 3-40-fold after all three kinds of stress. We examined transcription in cells from young and old individuals and from patients with Werner syndrome (WS), a segmental progeroid condition with a high incidence of cancer, and found various age-associated transcriptional changes depending upon the type of cellular stress. Compared to young individuals, both WS and old individuals had similarly aberrant transcriptional responses to gamma- and UV-irradiation, suggesting a role for Werner protein in stress-induced gene expression. Our results suggest that aberrant DNA damage-induced gene regulation may contribute to the aging process and the premature aging in WS. JF - Oncogene AU - Kyng, Kasper J AU - May, Alfred AU - Stevnsner, Tinna AU - Becker, Kevin G AU - Kølvrå, Steen AU - Bohr, Vilhelm A AD - Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. Y1 - 2005/07/28/ PY - 2005 DA - 2005 Jul 28 SP - 5026 EP - 5042 VL - 24 IS - 32 SN - 0950-9232, 0950-9232 KW - 4-nitroquinolone-1-oxide KW - 0 KW - Quinolones KW - 4-Nitroquinoline-1-oxide KW - 56-57-5 KW - Index Medicus KW - Fibroblasts -- drug effects KW - Ultraviolet Rays KW - Gamma Rays KW - Oligonucleotide Array Sequence Analysis KW - Genes, Immediate-Early -- radiation effects KW - Humans KW - Aged KW - Fibroblasts -- physiology KW - 4-Nitroquinoline-1-oxide -- pharmacology KW - Genes, Immediate-Early -- drug effects KW - Adult KW - Skin -- cytology KW - Fibroblasts -- radiation effects KW - Cell Line KW - Stress, Physiological KW - Quinolones -- pharmacology KW - Gene Expression Regulation -- radiation effects KW - Gene Expression Regulation -- drug effects KW - Werner Syndrome -- genetics KW - DNA Damage -- genetics KW - Aging -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68083263?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene&rft.atitle=Gene+expression+responses+to+DNA+damage+are+altered+in+human+aging+and+in+Werner+Syndrome.&rft.au=Kyng%2C+Kasper+J%3BMay%2C+Alfred%3BStevnsner%2C+Tinna%3BBecker%2C+Kevin+G%3BK%C3%B8lvr%C3%A5%2C+Steen%3BBohr%2C+Vilhelm+A&rft.aulast=Kyng&rft.aufirst=Kasper&rft.date=2005-07-28&rft.volume=24&rft.issue=32&rft.spage=5026&rft.isbn=&rft.btitle=&rft.title=Oncogene&rft.issn=09509232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-26 N1 - Date created - 2005-07-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of complex apurinic/apyrimidinic-site clustering associated with an authentic site-specific radiation-induced DNA double-strand break. AN - 68085584; 16024726 AB - Radiation lethality is largely attributed to radiation-induced DNA double-strand breaks (DSBs). A range of structural complexity is predicted for radiation-induced DSBs. However, this lesion has never been analyzed in isolation at the molecular level. To address this problem, we have created authentic site-specific radiation-induced DSBs in plasmid DNA by triplex-forming oligonucleotide-targeted 125I decay. No significant difference in DSB yield was observed after irradiation in the presence or absence of the radical scavenger DMSO, suggesting that DSB formation is a result of the direct effect of the radiation. A restriction fragment terminated by the DSB was isolated and probed with the Escherichia coli DNA repair enzyme endonuclease IV (endo IV), which recognizes apurinic/apyrimidinic (AP) sites. Enzymatic probing demonstrated clustering of AP sites within 10 bases of the 125I-targeted base in the DNA duplex. Our results suggest scavengeable radicals may not play a large role in the generation of AP sites associated with DSB formation, because at least 30% of all fragments have endo IV-sensitive sites, regardless of irradiation conditions. An internal control fragment recovered from the 125I linearized plasmid did not exhibit endo IV sensitivity in excess of that observed for a similar fragment recovered from an undamaged plasmid. Thus, AP site clustering proximal to the DSB resulted from the 125I decays responsible for DSB formation and was not due to untargeted background irradiation. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Datta, Kamal AU - Neumann, Ronald D AU - Winters, Thomas A AD - Nuclear Medicine Department, The Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07/26/ PY - 2005 DA - 2005 Jul 26 SP - 10569 EP - 10574 VL - 102 IS - 30 SN - 0027-8424, 0027-8424 KW - Escherichia coli Proteins KW - 0 KW - Iodine Radioisotopes KW - DNA KW - 9007-49-2 KW - Deoxyribonuclease IV (Phage T4-Induced) KW - EC 3.1.21.2 KW - endonuclease IV, E coli KW - DNA-(Apurinic or Apyrimidinic Site) Lyase KW - EC 4.2.99.18 KW - Dimethyl Sulfoxide KW - YOW8V9698H KW - Index Medicus KW - Escherichia coli Proteins -- metabolism KW - Base Sequence KW - DNA-(Apurinic or Apyrimidinic Site) Lyase -- metabolism KW - Deoxyribonuclease IV (Phage T4-Induced) -- metabolism KW - Plasmids -- genetics KW - DNA -- metabolism KW - Iodine Radioisotopes -- adverse effects KW - DNA -- radiation effects KW - DNA Damage -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68085584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Characterization+of+complex+apurinic%2Fapyrimidinic-site+clustering+associated+with+an+authentic+site-specific+radiation-induced+DNA+double-strand+break.&rft.au=Datta%2C+Kamal%3BNeumann%2C+Ronald+D%3BWinters%2C+Thomas+A&rft.aulast=Datta&rft.aufirst=Kamal&rft.date=2005-07-26&rft.volume=102&rft.issue=30&rft.spage=10569&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-29 N1 - Date created - 2005-07-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Acta Oncol. 1996;35(7):789-96 [9004754] Radiat Res. 2000 Mar;153(3):263-70 [10669547] Acta Oncol. 1996;35(7):817-23 [9004758] Nucleic Acids Res. 1997 Feb 15;25(4):883-7 [9016642] J Cell Biochem. 1997 Feb;64(2):258-72 [9027586] Int J Radiat Biol. 1997 Jul;72(1):91-100 [9246198] Antisense Nucleic Acid Drug Dev. 2000 Dec;10(6):443-52 [11198928] Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7426-30 [11404468] Radiat Res. 2001 Aug;156(2):158-66 [11448236] Radiat Res. 2001 Nov;156(5 Pt 2):577-83 [11604075] Int J Radiat Biol. 2002 Jun;78(6):457-66 [12065050] Nucleic Acids Res. 2002 Jul 1;30(13):2800-8 [12087163] J Am Chem Soc. 2002 Jul 31;124(30):8859-66 [12137539] Radiat Res. 2003 Feb;159(2):251-61 [12537531] Int J Radiat Biol. 2002 Dec;78(12):1095-102 [12556337] Anal Biochem. 2003 Jun 15;317(2):284-7 [12758272] Cold Spring Harb Symp Quant Biol. 2000;65:377-82 [12760053] Cancer Res. 2003 Sep 15;63(18):6008-15 [14522929] Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):489-505 [14565225] DNA Repair (Amst). 2004 Oct 5;3(10):1323-34 [15336627] Nucleic Acids Res. 2004;32(18):5609-20 [15494449] J Am Chem Soc. 1975 Apr 16;97(8):2277-8 [1133412] Radiat Res. 1981 May;86(2):185-95 [7015409] Science. 1981 Aug 21;213(4510):896-8 [7256283] J Biol Chem. 1982 Oct 10;257(19):11750-4 [7118909] J Biol Chem. 1983 Jan 25;258(2):711-3 [6822504] Radiat Res. 1985 Sep;103(3):383-92 [2994167] Radiat Res Suppl. 1985;8:S103-11 [3867077] Radiat Res. 1987 Jan;109(1):78-89 [3809393] Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 [2460825] Prog Nucleic Acid Res Mol Biol. 1988;35:95-125 [3065826] Int J Radiat Biol. 1991 Mar;59(3):625-42 [1672353] Int J Radiat Biol. 1994 Jan;65(1):7-17 [7905912] Int J Radiat Biol. 1994 Nov;66(5):427-32 [7983426] Nucleic Acids Res. 1994 Nov 25;22(23):4979-82 [7800489] Science. 1995 Feb 24;267(5201):1178-83 [7855601] Radiat Res. 1995 Oct;144(1):26-35 [7568768] Radiat Res. 2004 Dec;162(6):667-76 [15548117] Int J Radiat Biol. 1997 Sep;72(3):271-83 [9298107] Mutat Res. 1997 Sep;384(3):169-79 [9330613] Int J Radiat Biol. 1997 Oct;72(4):351-74 [9343102] J Nucl Med. 1998 Aug;39(8):1412-8 [9708519] C R Acad Sci III. 1999 Feb-Mar;322(2-3):89-101 [10196658] Chem Res Toxicol. 1999 Oct;12(10):917-23 [10525266] Int J Radiat Biol. 1999 Dec;75(12):1579-87 [10622264] Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):103-8 [10618378] Acta Oncol. 1996;35(7):797-801 [9004755] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ku86 preserves chromatin integrity in cells adapted to high NaCl. AN - 68084327; 16027367 AB - Cells adapted to high NaCl have many DNA breaks both in cell culture and in the renal inner medulla in vivo; yet they survive, function, and even proliferate. Here, we show that Ku86 is important for maintaining chromosomal integrity despite the continued presence of DNA breaks. The Ku heterodimer is part of DNA-dependent PK (DNA-PK), a complex that contributes by nonhomologous end joining to repair of double-strand breaks. We demonstrate that cells deficient in Ku86, but not cells deficient in DNA-PKcs (the catalytic subunit of DNA-PK), are hypersensitive to high NaCl as manifested by profound inhibition of proliferation, aberrant mitosis, and increased chromosomal fragmentation. Lower eukaryotes, including the soil nematode Caenorhabditis elegans, lack a DNA-PKcs homologue but are able to adapt to high NaCl. We show that cells of C. elegans adapted to high NaCl have many DNA breaks, similar to the mammalian cells adapted to high NaCl. Ku86 mutant C. elegans as well as C. elegans fed with cku86 dsRNA also display hypersensitivity to high NaCl, characterized by a reduced number of progeny and prolonged generation time in high NaCl. We propose that Ku86 ameliorates the effects of high NaCl-induced DNA breaks in adapted cells by supporting alignment of the broken ends of the DNA and thus maintaining integrity of the fragmented chromatin. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Dmitrieva, Natalia I AU - Celeste, Arkady AU - Nussenzweig, André AU - Burg, Maurice B AD - Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, and Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. dmitrien@nhlbi.nih.gov Y1 - 2005/07/26/ PY - 2005 DA - 2005 Jul 26 SP - 10730 EP - 10735 VL - 102 IS - 30 SN - 0027-8424, 0027-8424 KW - Antigens, Nuclear KW - 0 KW - Chromatin KW - DNA-Binding Proteins KW - Nuclear Proteins KW - Sodium Chloride KW - 451W47IQ8X KW - DNA-Activated Protein Kinase KW - EC 2.7.11.1 KW - Prkdc protein, mouse KW - Xrcc5 protein, mouse KW - EC 3.6.4.12 KW - Xrcc6 protein, human KW - Xrcc6 protein, mouse KW - Ku Autoantigen KW - EC 4.2.99.- KW - Index Medicus KW - Animals KW - Cytogenetic Analysis KW - Cricetulus KW - Reproduction -- drug effects KW - Humans KW - Mice KW - Blotting, Western KW - DNA-Activated Protein Kinase -- metabolism KW - Caenorhabditis elegans KW - RNA Interference KW - Nuclear Proteins -- metabolism KW - Cell Cycle -- drug effects KW - Cell Line KW - Cricetinae KW - DNA Damage KW - DNA-Binding Proteins -- genetics KW - Chromatin -- physiology KW - Antigens, Nuclear -- physiology KW - Antigens, Nuclear -- genetics KW - Chromatin -- genetics KW - DNA Repair -- genetics KW - DNA Repair -- physiology KW - Sodium Chloride -- toxicity KW - Antigens, Nuclear -- metabolism KW - Chromatin -- drug effects KW - DNA-Binding Proteins -- physiology KW - Adaptation, Physiological KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68084327?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Ku86+preserves+chromatin+integrity+in+cells+adapted+to+high+NaCl.&rft.au=Dmitrieva%2C+Natalia+I%3BCeleste%2C+Arkady%3BNussenzweig%2C+Andr%C3%A9%3BBurg%2C+Maurice+B&rft.aulast=Dmitrieva&rft.aufirst=Natalia&rft.date=2005-07-26&rft.volume=102&rft.issue=30&rft.spage=10730&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-29 N1 - Date created - 2005-07-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1999-2004 [11172065] Science. 2000 Dec 8;290(5498):1962-5 [11110662] Nature. 2001 Aug 9;412(6847):607-14 [11493912] Mutat Res. 2001 Sep 1;480-481:37-50 [11506797] Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):184-9 [11756692] Carcinogenesis. 2002 May;23(5):687-96 [12016139] Am J Physiol Renal Physiol. 2003 Aug;285(2):F266-74 [12684226] J Biol Chem. 2003 Oct 31;278(44):42729-32 [12912992] Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2317-22 [14983007] Am J Physiol Cell Physiol. 2004 Apr;286(4):C785-91 [14644776] Cell Cycle. 2004 May;3(5):561-3 [15107607] EMBO Rep. 2004 May;5(5):503-9 [15105825] Immunol Rev. 2004 Aug;200:132-41 [15242401] Mutat Res. 1983 Dec;112(6):313-27 [6197643] Radiat Res. 1993 Jun;134(3):349-54 [8316628] Am J Physiol. 1993 Sep;265(3 Pt 2):F416-24 [8214101] Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7623-7 [8052631] Science. 1994 Sep 2;265(5177):1442-5 [8073286] Science. 1994 Oct 14;266(5183):288-91 [7939667] Science. 1995 Feb 24;267(5201):1183-5 [7855602] Cell Prolif. 1995 Nov;28(11):571-9 [8555370] Nature. 1996 Aug 8;382(6591):551-5 [8700231] Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13588-93 [9391070] EMBO J. 1998 Jan 15;17(2):609-14 [9430651] Immunity. 1998 Sep;9(3):367-76 [9768756] Trends Biochem Sci. 1998 Oct;23(10):394-8 [9810228] Mol Cell Biol. 1999 May;19(5):3267-77 [10207052] Genes Dev. 1999 Apr 15;13(8):916-34 [10215620] Genes Cells. 1999 Feb;4(2):77-85 [10320474] Oncogene. 2005 Feb 3;24(6):949-61 [15592499] Nature. 2000 Mar 30;404(6777):510-4 [10761921] Genes Dev. 2000 Nov 15;14(22):2807-12 [11090128] Gene. 2001 Jan 24;263(1-2):103-12 [11223248] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A novel diacylglycerol-lactone shows marked selectivity in vitro among C1 domains of protein kinase C (PKC) isoforms alpha and delta as well as selectivity for RasGRP compared with PKCalpha. AN - 68054497; 15923197 AB - Although multiple natural products are potent ligands for the diacylglycerol binding C1 domain of protein kinase C (PKC), RasGRP, and related targets, the high conservation of C1 domains has impeded the development of selective ligands. We characterized here a diacylglycerol-lactone, 130C037, emerging from a combinatorial chemical synthetic strategy, which showed substantial selectivity. 130C037 gave shallow binding curves for PKC isoforms alpha, beta, gamma, delta, and epsilon, with apparent Ki values ranging from 340 nm for PKCalpha to 29 nm for PKCepsilon. When binding to isolated C1 domains of PKCalpha and -delta, 130C037 showed good affinity (Ki= 1.78 nm) only for deltaC1b, whereas phorbol 12,13-dibutyrate showed affinities within 10-fold for all. In LNCaP cells, 130C037 likewise selectively induced membrane translocation of deltaC1b. 130C037 bound intact RasGRP1 and RasGRP3 with Ki values of 3.5 and 3.8 nm, respectively, reflecting 8- and 90-fold selectivity relative to PKCepsilon and PKCalpha. By Western blot of Chinese hamster ovary cells, 130C037 selectively induced loss from the cytosol of RasGRP3 (ED50 = 286 nm), partial reduction of PKCepsilon (ED50 > 10 microm), and no effect on PKCalpha. As determined by confocal microscopy in LNCaP cells, 130C037 caused rapid translocation of RasGRP3, limited slow translocation of PKCepsilon, and no translocation of PKCalpha. Finally, 130C037 induced Erk phosphorylation in HEK-293 cells ectopically expressing RasGRP3 but not in control cells, whereas phorbol ester induced phosphorylation in both. The properties of 130C037 provide strong proof of principle for the feasibility of developing ligands with selectivity among C1 domain-containing therapeutic targets. JF - The Journal of biological chemistry AU - Pu, Yongmei AU - Perry, Nicholas A AU - Yang, Dazhi AU - Lewin, Nancy E AU - Kedei, Noemi AU - Braun, Derek C AU - Choi, Sung Hee AU - Blumberg, Peter M AU - Garfield, Susan H AU - Stone, James C AU - Duan, Dehui AU - Marquez, Victor E AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07/22/ PY - 2005 DA - 2005 Jul 22 SP - 27329 EP - 27338 VL - 280 IS - 29 SN - 0021-9258, 0021-9258 KW - 1,2-diacylglycerol KW - 0 KW - DNA-Binding Proteins KW - Diglycerides KW - Guanine Nucleotide Exchange Factors KW - Isoenzymes KW - Lactones KW - RASGRP1 protein, human KW - RASGRP3 protein, human KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - PRKCA protein, human KW - EC 2.7.11.13 KW - PRKCD protein, human KW - Protein Kinase C KW - Protein Kinase C-alpha KW - Protein Kinase C-delta KW - Index Medicus KW - Animals KW - Guanine Nucleotide Exchange Factors -- metabolism KW - Humans KW - Protein Binding KW - Binding Sites KW - Phorbol 12,13-Dibutyrate -- pharmacology KW - Phosphorylation -- drug effects KW - Cell Membrane -- metabolism KW - Cell Line KW - Protein Transport KW - DNA-Binding Proteins -- metabolism KW - Protein Kinase C -- metabolism KW - Diglycerides -- pharmacology KW - Protein Kinase C -- chemistry KW - Lactones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68054497?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=A+novel+diacylglycerol-lactone+shows+marked+selectivity+in+vitro+among+C1+domains+of+protein+kinase+C+%28PKC%29+isoforms+alpha+and+delta+as+well+as+selectivity+for+RasGRP+compared+with+PKCalpha.&rft.au=Pu%2C+Yongmei%3BPerry%2C+Nicholas+A%3BYang%2C+Dazhi%3BLewin%2C+Nancy+E%3BKedei%2C+Noemi%3BBraun%2C+Derek+C%3BChoi%2C+Sung+Hee%3BBlumberg%2C+Peter+M%3BGarfield%2C+Susan+H%3BStone%2C+James+C%3BDuan%2C+Dehui%3BMarquez%2C+Victor+E&rft.aulast=Pu&rft.aufirst=Yongmei&rft.date=2005-07-22&rft.volume=280&rft.issue=29&rft.spage=27329&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-09 N1 - Date created - 2005-07-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Heart rate variability under acute simulated microgravity during daytime waking state and nocturnal sleep: Comparison of horizontal and 6 degree head- down bed rest AN - 17392863; 6483019 AB - This study examined the acute effect of cephalad fluid shift under simulated microgravity on heart rate variability (HRV) during both daytime waking state and nocturnal sleep. Seven healthy male volunteers (21-31 years) underwent a series of experiments involving 6 degree head-down bed rest (HD) for 3 days. A control experiment on the same subjects was conducted under horizontal bed rest (HZ) in the same series. HRV from electrocardiogram signals was periodically calculated by the MemCalc method during daytime on the first and second days of both conditions. Nocturnal sleep on the first night of bed rest was monitored by polysomnography. HRV during stage 2 sleep and REM sleep were assessed in the former and latter halves of the sleep period time. Nocturnal sleep architecture under both conditions was normal, but a slight decrease in stage 4 sleep and an increase in the number of arousals occurred under HD. On both the first and second days, HRV during the daytime did not differ between HZ and HD. In contrast, high frequency components in HRV during sleep stage 2 were significantly higher in the latter half of sleep under HD than under HZ, although there were no differences in the ratio of low frequency to high frequency components during both stage 2 and the REM stage between the conditions. These results suggest that the acute effect of the cephalad fluid shift on cardiac autonomic nervous activity might be affected by the sleep/wake state modulating the dominance between sympathetic and parasympathetic nervous activity. JF - Neuroscience Letters AU - Mizuno, Koh AU - Inoue, Yuichi AU - Tanaka, Hideki AU - Komada, Yoko AU - Saito, Hidetomo AU - Mishima, Kazuo AU - Shirakawa, Shuichiro AD - Geriatric Mental Health, National Institute of Mental Health, National Center of Neurology and Psychiatry, Kohnodai 1-7-3, Ichikawa, Chiba 272- 0827, Japan Y1 - 2005/07/22/ PY - 2005 DA - 2005 Jul 22 SP - 115 EP - 120 VL - 383 IS - 1-2 SN - 0304-3940, 0304-3940 KW - Physical Education Index; CSA Neurosciences Abstracts KW - Sleep (REM) KW - Autonomic nervous system KW - Parasympathetic nervous system KW - Gravity KW - Arousal KW - Heart rate KW - Sympathetic nervous system KW - Health KW - Sleep and wakefulness KW - EKG KW - Dominance KW - Microgravity KW - Nervous system KW - Daytime KW - Sleep KW - Electrocardiology KW - Immobilization KW - N3 11140:Sleep and sleep disorders KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17392863?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+Letters&rft.atitle=Heart+rate+variability+under+acute+simulated+microgravity+during+daytime+waking+state+and+nocturnal+sleep%3A+Comparison+of+horizontal+and+6+degree+head-+down+bed+rest&rft.au=Mizuno%2C+Koh%3BInoue%2C+Yuichi%3BTanaka%2C+Hideki%3BKomada%2C+Yoko%3BSaito%2C+Hidetomo%3BMishima%2C+Kazuo%3BShirakawa%2C+Shuichiro&rft.aulast=Mizuno&rft.aufirst=Koh&rft.date=2005-07-22&rft.volume=383&rft.issue=1-2&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Neuroscience+Letters&rft.issn=03043940&rft_id=info:doi/10.1016%2Fj.neulet.2005.03.058 LA - English DB - Physical Education Index N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Nervous system; Arousal; Gravity; Sleep; Heart rate; Health; Electrocardiology; Immobilization; Dominance; Microgravity; Autonomic nervous system; Sleep (REM); Daytime; Parasympathetic nervous system; Sympathetic nervous system; Sleep and wakefulness; EKG DO - http://dx.doi.org/10.1016/j.neulet.2005.03.058 ER - TY - JOUR T1 - Gadolinium-Rhodamine Nanoparticles for Cell Labeling and Tracking via Magnetic Resonance and Optical Imaging AN - 19426154; 6486317 AB - A novel dual-labeled nanoparticle for use in labeling and tracking cells in vivo is described. We report the construction and characterization of these gadolinium-rhodamine nanoparticles. These particles are constructed from lipid monomers with diacetylene bonds that are sonicated and photolyzed to form polymerized nanoparticles. Cells are efficiently labeled with these nanoparticles. We have inoculated labeled tumor cells subcutaneouosly into the flanks of C3H mice and have been able to image these labeled tumor cells via MRI and optical imaging. Furthermore, the labeled tumor cells can be visualized via fluorescent microscopy after tissue biopsy. Our results suggest that these nanoparticles could be used to track cells in vivo. This basic platform can be modified with different fluorophores and targeting agents for studying metastisic cell, stem cell, and immune cell trafficking among other applications. JF - Bioconjugate Chemistry AU - Vuu, K AU - Xie, J AU - McDonald, MA AU - Bernardo, M AU - Hunter, F AU - Zhang, Y AU - Li, K AU - Bednarski, M AU - Guccione, S AD - Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, 4000 Jones Bridge Road, Chevy Chase, Maryland, 20815-6789, USA Y1 - 2005/07/20/ PY - 2005 DA - 2005 Jul 20 SP - 995 EP - 999 VL - 16 IS - 4 SN - 1043-1802, 1043-1802 KW - Biotechnology and Bioengineering Abstracts KW - Monomers KW - Stem cells KW - Lipids KW - Magnetic resonance imaging KW - N.M.R. KW - Biopsy KW - fluorophores KW - Tumor cells KW - nanoparticles KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19426154?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioconjugate+Chemistry&rft.atitle=Gadolinium-Rhodamine+Nanoparticles+for+Cell+Labeling+and+Tracking+via+Magnetic+Resonance+and+Optical+Imaging&rft.au=Vuu%2C+K%3BXie%2C+J%3BMcDonald%2C+MA%3BBernardo%2C+M%3BHunter%2C+F%3BZhang%2C+Y%3BLi%2C+K%3BBednarski%2C+M%3BGuccione%2C+S&rft.aulast=Vuu&rft.aufirst=K&rft.date=2005-07-20&rft.volume=16&rft.issue=4&rft.spage=995&rft.isbn=&rft.btitle=&rft.title=Bioconjugate+Chemistry&rft.issn=10431802&rft_id=info:doi/10.1021%2Fbc050085z LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - nanoparticles; Tumor cells; Stem cells; Lipids; Biopsy; Monomers; N.M.R.; fluorophores; Magnetic resonance imaging DO - http://dx.doi.org/10.1021/bc050085z ER - TY - CPAPER T1 - Dynamical Effects of Diffusive Cell Coupling on the Behavior of Pancreatic Beta-Cells. T2 - 6th European Conference on Mathematical and Theoretical Biology (ECMTB 2005) AN - 40006667; 3973850 JF - 6th European Conference on Mathematical and Theoretical Biology (ECMTB 2005) AU - Tsaneva-Atanasova, K AU - Sherman, A AU - Satin, L S AU - Nunemaker, C S AU - Zhang, M Y1 - 2005/07/18/ PY - 2005 DA - 2005 Jul 18 KW - Pancreas KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40006667?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+European+Conference+on+Mathematical+and+Theoretical+Biology+%28ECMTB+2005%29&rft.atitle=Dynamical+Effects+of+Diffusive+Cell+Coupling+on+the+Behavior+of+Pancreatic+Beta-Cells.&rft.au=Tsaneva-Atanasova%2C+K%3BSherman%2C+A%3BSatin%2C+L+S%3BNunemaker%2C+C+S%3BZhang%2C+M&rft.aulast=Tsaneva-Atanasova&rft.aufirst=K&rft.date=2005-07-18&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+European+Conference+on+Mathematical+and+Theoretical+Biology+%28ECMTB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.ecmtb05.org/php/schedule.php?listof=sessions LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Arfs, Phosphoinositides and Membrane Traffic T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 40041722; 3971636 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Donaldson, Julie G Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - phosphoinositides KW - membrane trafficking KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=Arfs%2C+Phosphoinositides+and+Membrane+Traffic&rft.au=Donaldson%2C+Julie+G&rft.aulast=Donaldson&rft.aufirst=Julie&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Most Infectious Scrapie Particle and Scrapie Prion Protein Trafficking in Neurons T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 40016859; 3971742 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Silveira, Jay AU - Magalhaes, Ana Cristina AU - Baron, Gerald S AU - Lee, Kil Sun AU - Prado, Marco AM AU - Caughey, Byron Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - Prion protein KW - Particulates KW - Neurons KW - Protein transport KW - Scrapie KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40016859?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=The+Most+Infectious+Scrapie+Particle+and+Scrapie+Prion+Protein+Trafficking+in+Neurons&rft.au=Silveira%2C+Jay%3BMagalhaes%2C+Ana+Cristina%3BBaron%2C+Gerald+S%3BLee%2C+Kil+Sun%3BPrado%2C+Marco+AM%3BCaughey%2C+Byron&rft.aulast=Silveira&rft.aufirst=Jay&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Unravelling the Reactions of Nitric Oxide, Nitrite and Haemoglobin T2 - Second Annual Meeting of the Biochemical Society (BioScience2005) AN - 39988728; 3971713 JF - Second Annual Meeting of the Biochemical Society (BioScience2005) AU - Gladwin, Mark T Y1 - 2005/07/17/ PY - 2005 DA - 2005 Jul 17 KW - Hemoglobin KW - Nitrite KW - Nitric oxide KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39988728?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.atitle=Unravelling+the+Reactions+of+Nitric+Oxide%2C+Nitrite+and+Haemoglobin&rft.au=Gladwin%2C+Mark+T&rft.aulast=Gladwin&rft.aufirst=Mark&rft.date=2005-07-17&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Second+Annual+Meeting+of+the+Biochemical+Society+%28BioScience2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.bioscience2005.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Pharmacological Stimulation of Beta2 Adrenergic Receptors(AR) Enhances Therapeutic Effectiveness of Beta1 Ar Blockade in Rodent Dilated Ischemic Cardiomyopathy T2 - 12th World Congress on Heart Disease - New Trends in Research, Diagnosis and Treatment AN - 40014011; 3942728 JF - 12th World Congress on Heart Disease - New Trends in Research, Diagnosis and Treatment AU - Ahmet, I AU - Lakatta, E G AU - Talan, M I Y1 - 2005/07/16/ PY - 2005 DA - 2005 Jul 16 KW - Cardiomyopathy KW - Ischemia KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40014011?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=12th+World+Congress+on+Heart+Disease+-+New+Trends+in+Research%2C+Diagnosis+and+Treatment&rft.atitle=Pharmacological+Stimulation+of+Beta2+Adrenergic+Receptors%28AR%29+Enhances+Therapeutic+Effectiveness+of+Beta1+Ar+Blockade+in+Rodent+Dilated+Ischemic+Cardiomyopathy&rft.au=Ahmet%2C+I%3BLakatta%2C+E+G%3BTalan%2C+M+I&rft.aulast=Ahmet&rft.aufirst=I&rft.date=2005-07-16&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=12th+World+Congress+on+Heart+Disease+-+New+Trends+in+Research%2C+Diagnosis+and+Treatment&rft.issn=&rft_id=info:doi/ L2 - http://www.cardiologyonline.com/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Evaluation of radiation-induced oral mucositis by optical coherence tomography. AN - 68056535; 16033826 AB - Optical coherence tomography (OCT) imaging was evaluated to determine if radiation-induced mucosal damage could be noninvasively monitored in real time and correlated with histopathologic findings. Female C3H mice, ages 7 to 9 weeks, four per group, were immobilized in a custom-made Lucite jig and received 0, 15, 22.5, and 25 Gy in a single fraction to their oral cavity. OCT images were acquired of proximal, middle, and distal aspects of the dorsum of the tongue on days 0, 1, 3, 5, and 7 post-irradiation. Animals were sacrificed on day 7 and samples taken for histologic evaluation. OCT images were visually examined and also quantified by image analysis and compared with histologic findings. Tongues removed 7 days post-irradiation showed no visible damage; however, upon staining with toluidine blue, ulcers at the base of the tongue became visible (100% for 25 Gy, 75% after 22.5 Gy, and 0% after 15 Gy). Visual inspection of OCT images qualitatively compared with histologic findings and quantitative image analysis of the OCT images (effective light penetration depth) revealed significant changes 7 days post-irradiation compared with unirradiated controls for the base of the tongue. OCT allows for direct noninvasive real-time acquisition of digitally archivable images of oral mucosa and can detect radiation-induced changes in the mucosa before visual manifestation. OCT may be a useful technique to quantify subclinical radiation-induced mucosal injury in experimental chemoradiation clinical trials. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Muanza, Thierry M AU - Cotrim, Ana P AU - McAuliffe, Mathew AU - Sowers, Anastasia L AU - Baum, Bruce J AU - Cook, John A AU - Feldchtein, Felix AU - Amazeen, Paul AU - Coleman, C Norman AU - Mitchell, James B AD - Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 5121 EP - 5127 VL - 11 IS - 14 SN - 1078-0432, 1078-0432 KW - Index Medicus KW - Severity of Illness Index KW - Sensitivity and Specificity KW - Animals KW - Mouth Mucosa KW - Disease Models, Animal KW - Mice KW - Female KW - Radiation Injuries -- veterinary KW - Stomatitis -- veterinary KW - Stomatitis -- etiology KW - Stomatitis -- diagnosis KW - Tomography, Optical Coherence KW - Radiation Injuries -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Evaluation+of+radiation-induced+oral+mucositis+by+optical+coherence+tomography.&rft.au=Muanza%2C+Thierry+M%3BCotrim%2C+Ana+P%3BMcAuliffe%2C+Mathew%3BSowers%2C+Anastasia+L%3BBaum%2C+Bruce+J%3BCook%2C+John+A%3BFeldchtein%2C+Felix%3BAmazeen%2C+Paul%3BColeman%2C+C+Norman%3BMitchell%2C+James+B&rft.aulast=Muanza&rft.aufirst=Thierry&rft.date=2005-07-15&rft.volume=11&rft.issue=14&rft.spage=5121&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-07 N1 - Date created - 2005-07-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. AN - 68045834; 16024603 AB - Programmed cell death 4 (Pdcd4) is a novel repressor of in vitro transformation. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A, a component of the translation initiation complex. To ascertain whether Pdcd4 suppresses tumor development in vivo, we have generated transgenic mice that overexpress Pdcd4 in the epidermis (K14-Pdcd4). K14-regulated Pdcd4 expression caused a neonatal short-hair phenotype due to early catagen entry compared with matched wild-type siblings. In response to the 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA) mouse skin carcinogenesis protocol, K14-Pdcd4 mice showed significant reductions in papilloma formation, carcinoma incidence, and papilloma-to-carcinoma conversion frequency compared with wild-type mice. The translational efficiency of an mRNA engineered to form a structured 5' untranslated region (UTR) was attenuated in primary keratinocytes when Pdcd4 was overexpressed. Pdcd4 inhibited by 46% TPA-induced activator protein-1 (AP-1)-dependent transcription, an event required for tumorigenesis. CDK4 and ornithine decarboxylase (ODC) are candidates for Pdcd4-regulated translation as their mRNAs contain 5'structured UTRs. In K14-Pdcd4 primary keratinocytes expressing activated Ha-Ras to mimic DMBA-initiated epidermis, ODC and CDK4 protein levels were decreased by 40% and 46%, respectively. Expression of a protein encoded by 5' unstructured mRNA showed no change. These results extend to an in vivo model the observations that Pdcd4 inhibits both translation initiation and AP-1 activation while decreasing benign tumor development and malignant progression. The K14-Pdcd4 mice seem to validate translation initiation as a novel target for cancer prevention. JF - Cancer research AU - Jansen, Aaron P AU - Camalier, Corinne E AU - Colburn, Nancy H AD - Gene Regulation Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA. ajansen@ncifcrf.gov Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 6034 EP - 6041 VL - 65 IS - 14 SN - 0008-5472, 0008-5472 KW - 5' Untranslated Regions KW - 0 KW - Apoptosis Regulatory Proteins KW - Pdcd4 protein, mouse KW - RNA, Messenger KW - RNA-Binding Proteins KW - Transcription Factor AP-1 KW - Luciferases KW - EC 1.13.12.- KW - Index Medicus KW - Protein Biosynthesis KW - Animals KW - Transcription Factor AP-1 -- antagonists & inhibitors KW - Epidermis -- metabolism KW - Disease Progression KW - Mice KW - RNA, Messenger -- genetics KW - Mice, Transgenic KW - Transcription Factor AP-1 -- physiology KW - Transcriptional Activation KW - RNA, Messenger -- biosynthesis KW - Luciferases -- biosynthesis KW - Promoter Regions, Genetic KW - Cytoplasm -- metabolism KW - Luciferases -- antagonists & inhibitors KW - Epidermis -- pathology KW - Luciferases -- genetics KW - Female KW - Papilloma -- pathology KW - Papilloma -- prevention & control KW - RNA-Binding Proteins -- genetics KW - RNA-Binding Proteins -- physiology KW - Skin Neoplasms -- pathology KW - Carcinoma, Squamous Cell -- metabolism KW - Papilloma -- genetics KW - Skin Neoplasms -- prevention & control KW - Skin Neoplasms -- metabolism KW - Skin Neoplasms -- genetics KW - RNA-Binding Proteins -- biosynthesis KW - Carcinoma, Squamous Cell -- pathology KW - Carcinoma, Squamous Cell -- genetics KW - Carcinoma, Squamous Cell -- prevention & control KW - Papilloma -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68045834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Epidermal+expression+of+the+translation+inhibitor+programmed+cell+death+4+suppresses+tumorigenesis.&rft.au=Jansen%2C+Aaron+P%3BCamalier%2C+Corinne+E%3BColburn%2C+Nancy+H&rft.aulast=Jansen&rft.aufirst=Aaron&rft.date=2005-07-15&rft.volume=65&rft.issue=14&rft.spage=6034&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-14 N1 - Date created - 2005-07-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of claudin-3 at threonine 192 by cAMP-dependent protein kinase regulates tight junction barrier function in ovarian cancer cells. AN - 68020519; 15905176 AB - Claudins are integral membrane proteins essential in the formation and function of tight junctions (TJs). Disruption of TJs, which have essential roles in cell permeability and polarity, is thought to contribute to epithelial tumorigenesis. Claudin-3 and -4 are frequently overexpressed in ovarian cancer, but the molecular pathways involved in the regulation of these proteins are unclear. Interestingly, several studies have demonstrated a role for phosphorylation in the regulation of TJ complexes, although evidence for claudin phosphorylation is scarce. Here, we showed that claudin-3 and -4 can be phosphorylated in ovarian cancer cells. In vitro phosphorylation assays using glutathione S-transferase fusion constructs demonstrated that the C terminus of claudin-3 is an excellent substrate for cAMP-dependent protein kinase (PKA). Using site-directed mutagenesis, we identified a PKA phosphorylation site at amino acid 192 in the C terminus of claudin-3. Overexpression of the protein containing a T192D mutation, mimicking the phosphorylated state, resulted in a decrease in TJ strength in ovarian cancer cell line OVCA433. Our results suggest that claudin-3 phosphorylation by PKA, a kinase frequently activated in ovarian cancer, may provide a mechanism for the disruption of TJs in this cancer. In addition, our findings may have general implications for the regulation of TJs in normal epithelial cells. JF - The Journal of biological chemistry AU - D'Souza, Theresa AU - Agarwal, Rachana AU - Morin, Patrice J AD - Laboratory of Cellular and Molecular Biology, Gerontology Research Center, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 26233 EP - 26240 VL - 280 IS - 28 SN - 0021-9258, 0021-9258 KW - CLDN3 protein, human KW - 0 KW - CLDN4 protein, human KW - Claudin-3 KW - Claudin-4 KW - Enzyme Inhibitors KW - Membrane Proteins KW - Recombinant Fusion Proteins KW - Recombinant Proteins KW - Threonine KW - 2ZD004190S KW - Glutathione Transferase KW - EC 2.5.1.18 KW - Cyclic AMP-Dependent Protein Kinases KW - EC 2.7.11.11 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Recombinant Fusion Proteins -- chemistry KW - Calcium -- metabolism KW - Microscopy, Fluorescence KW - Recombinant Fusion Proteins -- metabolism KW - Permeability KW - Phosphorylation KW - Recombinant Proteins -- chemistry KW - Time Factors KW - Immunoblotting KW - Electric Impedance KW - Immunoprecipitation KW - Cell Line, Tumor KW - Electrophysiology KW - Binding Sites KW - Transfection KW - Models, Genetic KW - Enzyme Inhibitors -- pharmacology KW - Protein Structure, Tertiary KW - Immunohistochemistry KW - Mutation KW - Female KW - Cell Line KW - Cyclic AMP-Dependent Protein Kinases -- metabolism KW - Ovarian Neoplasms -- metabolism KW - Threonine -- chemistry KW - Cyclic AMP-Dependent Protein Kinases -- physiology KW - Membrane Proteins -- chemistry KW - Membrane Proteins -- metabolism KW - Glutathione Transferase -- metabolism KW - Cyclic AMP-Dependent Protein Kinases -- chemistry KW - Tight Junctions -- chemistry KW - Membrane Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68020519?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Phosphorylation+of+claudin-3+at+threonine+192+by+cAMP-dependent+protein+kinase+regulates+tight+junction+barrier+function+in+ovarian+cancer+cells.&rft.au=D%27Souza%2C+Theresa%3BAgarwal%2C+Rachana%3BMorin%2C+Patrice+J&rft.aulast=D%27Souza&rft.aufirst=Theresa&rft.date=2005-07-15&rft.volume=280&rft.issue=28&rft.spage=26233&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-12 N1 - Date created - 2005-07-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The Ins(1,3,4)P3 5/6-kinase/Ins(3,4,5,6)P4 1-kinase is not a protein kinase. AN - 67997088; 15762844 AB - Among inositol phosphate kinases, Ins(3,4,5,6)P4 1-kinase has been considered to be an outsider with disparate sequence, a proclaimed capacity to also phosphorylate proteins and apparent 1-phosphatase activity. Such multifunctionality, coupled with ignorance of its operational domains, complicates any mechanistic rationale behind literature reports that Ins(3,4,5,6)P4 1-kinase regulates apoptosis, salt and fluid secretion, and transcription. We have expressed poly(His)-tagged human Ins(3,4,5,6)P4 1-kinase in Sf9 insect cells and purified the enzyme using Ni-agarose chromatography. Protein kinase activity was eluted from the Ni-agarose column, but this did not co-elute with the Ins(3,4,5,6)P4 1-kinase, indicating that the protein kinase and inositol kinase activities belong to separate proteins. To pursue this conclusion, we prepared catalytically inactive mutants of the Ins(3,4,5,6)P4 1-kinase by identifying and targeting the ATP-binding site. Our strategy was based on sequence alignments suggesting homology of the Ins(3,4,5,6)P4 1-kinase with ATP-grasp metabolic enzymes. Individual mutation of four candidate MgATP-binding participants, Lys157, Asp281, Asp295 and Asn297, severely compromised Ins(3,4,5,6)P4 1-kinase activity. Yet, these mutations did not affect the protein kinase activity. We conclude that the Ins(3,4,5,6)P4 1-kinase is not a protein kinase, contrary to earlier reports [e.g. Wilson, Sun, Cao and Majerus (2001) J. Biol. Chem. 276, 40998-41004]. Elimination of protein kinase activity from the enzyme's repertoire and recognition of its ATP-grasp homology together indicate that structural, functional and catalytic relationships between Ins(3,4,5,6)P4 1-kinase and other inositol phosphate kinases are closer than previously thought [Gonzalez, Schell, Letcher, Veprintsev, Irvine and Williams (2004) Mol. Cell 15, 689-701]. JF - The Biochemical journal AU - Qian, Xun AU - Mitchell, Jennifer AU - Wei, Sung-Jen AU - Williams, Jason AU - Petrovich, Robert M AU - Shears, Stephen B AD - Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHSS, Research Triangle Park, PO Box 12233, NC 27709, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 389 EP - 395 VL - 389 KW - Recombinant Proteins KW - 0 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Phosphotransferases (Alcohol Group Acceptor) KW - EC 2.7.1.- KW - inositol-tetrakisphosphate 1-kinase KW - myo-inositol-trisphosphate 6-kinase KW - EC 2.7.1.134 KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Animals KW - Conserved Sequence KW - Humans KW - Adenosine Triphosphate -- metabolism KW - Amino Acid Sequence KW - Substrate Specificity KW - Protein Structure, Tertiary KW - Cell Line KW - Catalysis KW - Binding Sites KW - Phosphotransferases (Alcohol Group Acceptor) -- chemistry KW - Phosphotransferases (Alcohol Group Acceptor) -- metabolism KW - Phosphotransferases (Alcohol Group Acceptor) -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67997088?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Biochemical+journal&rft.atitle=The+Ins%281%2C3%2C4%29P3+5%2F6-kinase%2FIns%283%2C4%2C5%2C6%29P4+1-kinase+is+not+a+protein+kinase.&rft.au=Qian%2C+Xun%3BMitchell%2C+Jennifer%3BWei%2C+Sung-Jen%3BWilliams%2C+Jason%3BPetrovich%2C+Robert+M%3BShears%2C+Stephen+B&rft.aulast=Qian&rft.aufirst=Xun&rft.date=2005-07-15&rft.volume=389&rft.issue=&rft.spage=389&rft.isbn=&rft.btitle=&rft.title=The+Biochemical+journal&rft.issn=1470-8728&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-21 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 2003 Oct 31;278(44):43645-53 [12925536] Cell. 2003 Sep 19;114(6):663-71 [14505567] Mol Cell. 2004 Sep 10;15(5):689-701 [15350214] Mol Cell. 2004 Sep 10;15(5):703-11 [15350215] Science. 1994 Oct 21;266(5184):439-43 [7939684] Biochemistry. 1995 Mar 7;34(9):2768-76 [7893688] J Biol Chem. 1996 Jun 14;271(24):14092-7 [8662902] Curr Opin Struct Biol. 1996 Jun;6(3):386-94 [8804825] Biochemistry. 1996 Nov 12;35(45):14352-61 [8916922] Biochemistry. 1996 Nov 12;35(45):14362-9 [8916923] Protein Sci. 1997 Dec;6(12):2639-43 [9416615] Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13597-602 [9811845] Biochemistry. 1999 Feb 23;38(8):2347-57 [10029528] Biochemistry. 1999 Mar 16;38(11):3393-400 [10079084] J Biol Chem. 2000 May 26;275(21):16183-90 [10821865] J Mol Biol. 2000 Jun 2;299(2):499-520 [10860755] Biochem J. 2000 Nov 1;351 Pt 3:551-5 [11042108] Biochemistry. 2000 Dec 26;39(51):15895-900 [11123916] Nat Rev Mol Cell Biol. 2001 May;2(5):327-38 [11331907] J Biol Chem. 2001 Jul 6;276(27):24991-6 [11346647] Trends Cell Biol. 2001 Oct;11(10):420-6 [11567875] J Biol Chem. 2001 Nov 2;276(44):40998-1004 [11533064] Curr Biol. 2002 Mar 19;12(6):477-82 [11909533] J Mol Biol. 2002 Jul 19;320(4):855-81 [12095261] J Biol Chem. 2002 Jul 26;277(30):26717-20 [12055181] J Biol Chem. 2002 Nov 15;277(46):43836-43 [12223481] J Biol Chem. 2002 Nov 29;277(48):45759-64 [12324474] EMBO J. 2003 Mar 17;22(6):1302-12 [12628923] Trends Genet. 2003 Aug;19(8):415-7 [12902157] Biochem J. 2004 Jan 15;377(Pt 2):265-80 [14567754] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization. AN - 67996919; 15743274 AB - All 20 cysteine residues are accessible to disulphide reagents in the tubulin dimer, whereas only four are accessible in taxol-stabilized microtubules. Reaction rates with disulphide reagents are a function of the reagent, are decreased by G nucleotides, and increased with increase in pH and urea. With transient (stop-flow) kinetics, DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)] and 2,2'-dithiodipyridine progress curves cannot be fitted by the sum of exponential terms based only on classes of cysteines. The mixed disulphide products react further to form both intra- and intermonomer disulphide bonds that can be reversed by reducing agents. With MMTS (methyl methanethiosulphonate) or ODNB (n-octyl-dithio-2-nitrobenzoate), virtually no protein-protein disulphide bonds are formed and the ODNB reaction can be given as the sum of three exponential terms with pseudo-first-order rate constants of 0.206, 0.069 and 0.010 s(-1) at pH 6.5, suggesting three classes of thiol reactivities. Limited cysteine substitution leads to only small changes in tryptophan or CD spectra, whereas complete substitution leads to loss of the helix content. MMTS-induced loss of SH groups leads to progressive increases in the critical concentration and loss of polymerization competence that can be reversed by assembly promoters such as higher protein concentration, taxol or high ionic strength. Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles. JF - The Biochemical journal AU - Britto, P J AU - Knipling, Leslie AU - McPhie, Peter AU - Wolff, J AD - Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 549 EP - 558 VL - 389 KW - Biopolymers KW - 0 KW - Disulfides KW - Nitrobenzoates KW - Sulfhydryl Compounds KW - Tubulin KW - Guanosine Diphosphate KW - 146-91-8 KW - n-octyl-5-dithio-2-nitrobenzoic acid KW - 146019-29-6 KW - thionitrobenzoic acid KW - 15139-21-6 KW - 2,2'-dipyridyl disulfide KW - 2127-03-9 KW - methyl methanethiosulfonate KW - 2949-92-0 KW - 2,2'-Dipyridyl KW - 551W113ZEP KW - Guanosine Triphosphate KW - 86-01-1 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Dithionitrobenzoic Acid KW - 9BZQ3U62JX KW - Methyl Methanesulfonate KW - AT5C31J09G KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Molecular Structure KW - Biopolymers -- chemistry KW - Biopolymers -- metabolism KW - Cysteine -- metabolism KW - Guanosine Diphosphate -- metabolism KW - Protein Denaturation KW - 2,2'-Dipyridyl -- analogs & derivatives KW - Amino Acid Sequence KW - Methyl Methanesulfonate -- analogs & derivatives KW - Guanosine Triphosphate -- metabolism KW - Methyl Methanesulfonate -- chemistry KW - Cysteine -- chemistry KW - Dithionitrobenzoic Acid -- chemistry KW - Kinetics KW - Adenosine Triphosphate -- metabolism KW - 2,2'-Dipyridyl -- chemistry KW - Nitrobenzoates -- chemistry KW - Disulfides -- chemistry KW - Sulfhydryl Compounds -- metabolism KW - Sulfhydryl Compounds -- chemistry KW - Tubulin -- chemistry KW - Tubulin -- metabolism KW - Disulfides -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67996919?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Biochemical+journal&rft.atitle=Thiol-disulphide+interchange+in+tubulin%3A+kinetics+and+the+effect+on+polymerization.&rft.au=Britto%2C+P+J%3BKnipling%2C+Leslie%3BMcPhie%2C+Peter%3BWolff%2C+J&rft.aulast=Britto&rft.aufirst=P&rft.date=2005-07-15&rft.volume=389&rft.issue=&rft.spage=549&rft.isbn=&rft.btitle=&rft.title=The+Biochemical+journal&rft.issn=1470-8728&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-21 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Anal Biochem. 1979 Apr 1;94(1):75-81 [37780] Eur J Biochem. 1976 Sep;68(1):313-9 [964268] J Cell Biol. 1981 Apr;89(1):45-53 [7228899] Biochemistry. 1981 Jul 21;20(15):4437-44 [7284333] Biochim Biophys Acta. 1981 Nov 30;671(1):71-7 [7306573] Biochemistry. 1983 Mar 29;22(7):1567-72 [6849867] Biochim Biophys Acta. 1989 Jun 13;996(1-2):110-5 [2736254] Ital J Biochem. 1989 Mar-Apr;38(2):83-90 [2745039] Oxf Surv Eukaryot Genes. 1984;1:36-60 [6400775] Biochemistry. 1989 Nov 14;28(23):9143-52 [2605248] Adv Enzymol Relat Areas Mol Biol. 1990;63:69-172 [2407068] Biochim Biophys Acta. 1991 Jan 29;1076(2):289-97 [1998728] Pharmacol Ther. 1991;49(1-2):133-52 [1852786] J Biol Chem. 1993 Jan 5;268(1):127-32 [8416920] Anal Biochem. 1993 Feb 1;208(2):357-62 [8452233] Biochemistry. 1994 Nov 1;33(43):12868-78 [7947693] Protein Sci. 1995 Nov;4(11):2411-23 [8563639] Biochemistry. 1996 May 7;35(18):5910-20 [8639553] Nat Struct Biol. 1997 Jun;4(6):450-5 [9187652] Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3661-6 [9520422] J Biol Chem. 1998 Jul 24;273(30):19198-206 [9668107] Eur J Biochem. 2000 Jun;267(12):3469-76 [10848962] Biochemistry. 2001 Jul 31;40(30):8834-41 [11467944] J Mol Biol. 2001 Nov 9;313(5):1045-57 [11700061] Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1353-8 [11805304] Arch Biochem Biophys. 2002 Feb 15;398(2):213-20 [11831852] J Biol Chem. 2002 Aug 9;277(32):29018-27 [12023292] Arch Biochem Biophys. 2002 Oct 15;406(2):229-40 [12361711] Anal Biochem. 2003 Jan 15;312(2):224-7 [12531209] Biochim Biophys Acta. 2003 Apr 8;1631(3):239-45 [12668175] Biochem Biophys Res Commun. 2004 Feb 6;314(2):555-60 [14733943] Free Radic Biol Med. 2004 Feb 15;36(4):497-506 [14975452] J Biol Chem. 2004 May 21;279(21):21749-58 [15031298] J Biol Chem. 1967 Jun 10;242(11):2709-18 [6027244] Biochem J. 1973 May;133(1):67-80 [4721623] Biochemistry. 1973 Oct 9;12(21):4282-9 [4745672] J Mol Biol. 1974 Nov 15;89(4):737-55 [4475698] Biochemistry. 1981 Jan 6;20(1):33-7 [7470476] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Bcl-x and Stat5 Modulate Erythropoiesis through Distinct, Stagespecific Mechanisms T2 - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AN - 40107978; 3982025 JF - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AU - Zhu, Bing-Mei AU - Cui, Yongzhi AU - Heinrich, Achim AU - Klingmuller, Ursula AU - Liu, Jie AU - Robinson, Gertraud W AU - Hennighausen, Lothar Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 KW - Erythropoiesis KW - Bcl-x protein KW - Stat5 protein KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107978?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.atitle=Bcl-x+and+Stat5+Modulate+Erythropoiesis+through+Distinct%2C+Stagespecific+Mechanisms&rft.au=Zhu%2C+Bing-Mei%3BCui%2C+Yongzhi%3BHeinrich%2C+Achim%3BKlingmuller%2C+Ursula%3BLiu%2C+Jie%3BRobinson%2C+Gertraud+W%3BHennighausen%2C+Lothar&rft.aulast=Zhu&rft.aufirst=Bing-Mei&rft.date=2005-07-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.issn=&rft_id=info:doi/ L2 - http://www.ascb.org/meetings/summer05/ecb/index.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Integrin Signaling and Local Remodeling T2 - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AN - 40022553; 3982016 JF - 2005 ASCB Summer Meeting on Engineering Cell Biology: The Cell In Context AU - Yamada, Ken Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 KW - Integrins KW - Signal transduction KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40022553?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.atitle=Integrin+Signaling+and+Local+Remodeling&rft.au=Yamada%2C+Ken&rft.aulast=Yamada&rft.aufirst=Ken&rft.date=2005-07-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+ASCB+Summer+Meeting+on+Engineering+Cell+Biology%3A+The+Cell+In+Context&rft.issn=&rft_id=info:doi/ L2 - http://www.ascb.org/meetings/summer05/ecb/index.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Spontaneous differentiation of mouse embryonic stem cells in vitro: Characterization by global gene expression profiles AN - 19485359; 8250490 AB - We characterized the temporal gene expression changes during four weeks of spontaneous differentiation of mouse ES cells in a monolayer culture in order to obtain better insight into the differentiation process. The overall gene expression pattern was changed dramatically during the first two weeks of spontaneous differentiation, but stabilized after the second week. Most of the genes regulated within the first two weeks of spontaneous differentiation were genes related to development including morphogenesis, cell differentiation, embryonic development, pattern specification, mesoderm development, post-embryonic development, and blastocyst development. While most of the ectoderm lineage related genes were down-regulated, genes related to the mesoderm or endoderm lineage were up-regulated through the first week and second week, respectively. This study revealed that the development of ectoderm lineage is a recessive process during the spontaneous differentiation of mouse ES cells in monolayer culture. Our time-course characterization might provide a useful time line for directed differentiation of mouse ES cells. JF - Biochemical and Biophysical Research Communications AU - Heo, J AU - Lee, J S AU - Chu, I S AU - Takahama, Y AU - Thorgeirsson, S S AD - Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA, snorri_s_thorgeirsson@nih.gov Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 1061 EP - 1069 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 332 IS - 4 SN - 0006-291X, 0006-291X KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Gene expression KW - Differentiation KW - Stem cells KW - blastocysts KW - Embryogenesis KW - Embryo cells KW - Ectoderm KW - Morphogenesis KW - Cell culture KW - Endoderm KW - Mesoderm KW - G 07730:Development & Cell Cycle KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19485359?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+and+Biophysical+Research+Communications&rft.atitle=Spontaneous+differentiation+of+mouse+embryonic+stem+cells+in+vitro%3A+Characterization+by+global+gene+expression+profiles&rft.au=Heo%2C+J%3BLee%2C+J+S%3BChu%2C+I+S%3BTakahama%2C+Y%3BThorgeirsson%2C+S+S&rft.aulast=Heo&rft.aufirst=J&rft.date=2005-07-15&rft.volume=332&rft.issue=4&rft.spage=1061&rft.isbn=&rft.btitle=&rft.title=Biochemical+and+Biophysical+Research+Communications&rft.issn=0006291X&rft_id=info:doi/10.1016%2Fj.bbrc.2005.04.173 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Gene expression; Differentiation; Embryogenesis; blastocysts; Stem cells; Embryo cells; Ectoderm; Morphogenesis; Endoderm; Cell culture; Mesoderm DO - http://dx.doi.org/10.1016/j.bbrc.2005.04.173 ER - TY - JOUR T1 - Fetal Alcohol Spectrum Disorders AN - 17667367; 6525020 AB - To complement the 2005 Annual Clinical Focus on medical genomics, AFP will be publishing a series of short reviews on genetic syndromes. This series was designed to increase awareness of these diseases so that family physicians can recognize and diagnose children with these disorders and understand the kind of care they might require in the future. The second review in this series discusses fetal alcohol syndrome and fetal alcohol spectrum disorders. JF - American Family Physician AU - Wattendorf, D J AU - Muenke, M AD - National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 279 EP - 282 VL - 72 IS - 2 SN - 0002-838X, 0002-838X KW - Toxicology Abstracts KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17667367?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Family+Physician&rft.atitle=Fetal+Alcohol+Spectrum+Disorders&rft.au=Wattendorf%2C+D+J%3BMuenke%2C+M&rft.aulast=Wattendorf&rft.aufirst=D&rft.date=2005-07-15&rft.volume=72&rft.issue=2&rft.spage=279&rft.isbn=&rft.btitle=&rft.title=American+Family+Physician&rft.issn=0002838X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Recent advances in biomarkers for cancer diagnosis and treatment AN - 17543392; 6412410 AB - Biomarker advances, benefiting from technological revolutions in the field of proteomics, genonomics, and epigenomics, offer hopes that one day cancer diagnosis and treatment will be guided by and individualized at the molecular levels. JF - Drug Discovery Today AU - Manne, U AU - Srivastava, R G AU - Srivastava, S Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 965 EP - 976 PB - Elsevier Ltd VL - 10 IS - 14 SN - 1359-6446, 1359-6446 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Drug discovery KW - Reviews KW - proteomics KW - biomarkers KW - Cancer KW - W 30965:Miscellaneous, Reviews KW - W3 33000:General topics and reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17543392?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+Discovery+Today&rft.atitle=Recent+advances+in+biomarkers+for+cancer+diagnosis+and+treatment&rft.au=Manne%2C+U%3BSrivastava%2C+R+G%3BSrivastava%2C+S&rft.aulast=Manne&rft.aufirst=U&rft.date=2005-07-15&rft.volume=10&rft.issue=14&rft.spage=965&rft.isbn=&rft.btitle=&rft.title=Drug+Discovery+Today&rft.issn=13596446&rft_id=info:doi/10.1016%2FS1359-6446%2805%2903487-2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; biomarkers; proteomics; Reviews; Drug discovery DO - http://dx.doi.org/10.1016/S1359-6446(05)03487-2 ER - TY - JOUR T1 - Effector Functions of Heparin-Binding Hemagglutinin-Specific CD8 super(+) T Lymphocytes in Latent Human Tuberculosis AN - 17502536; 6385800 AB - Background. Most individuals infected with Mycobacterium tuberculosis do not develop tuberculosis (TB) and can be regarded as being protected by an appropriate immune response to the infection. The characterization of the immune responses of individuals with latent TB may thus be helpful in the definition of correlates of protection and the development of new vaccine strategies. The highly protective antigen heparin-binding hemagglutinin (HBHA) induces strong interferon (IFN)- gamma responses during latent, but not active, TB. Because of the recently recognized importance of CD8 super(+) T lymphocytes in anti-TB immunity, we characterized the CD8 super(+) T lymphocyte responses to HBHA in subjects with latent TB. Results. HBHA-specific CD8 super(+) T lymphcoytes expressed memory cell markers and synthesized HBHA-specific IFN- gamma . They also restricted mycobacterial growth and expressed cytotoxicity by a granule-dependent mechanism. This activity was associated with the intracellular expression of HBHA-induced perforin. Surprisingly, the perforin-producing CD8 super(+) T lymphocytes were distinct from the IFN- gamma -producing CD8 super(+) T lymphocytes. Conclusion. During latent TB, the HBHA-specific CD8 super(+) T lymphocyte population expresses all 3 effector functions associated with CD8 super(+) T lymphocyte-mediated protective immune mechanisms, which supports the notion that HBHA may be protective in humans and suggests that markers of HBHA-specific CD8 super(+) T lymphcoyte responses may be useful in the monitoring of protection. JF - Journal of Infectious Diseases AU - Temmerman, ST AU - Place, S AU - Debrie, A-S AU - Locht, C AU - Mascart, F AD - Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - 226 EP - 232 VL - 192 IS - 2 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology KW - Perforin KW - Hemagglutinins KW - protective antigen KW - Memory cells KW - Immunity KW - CD8 antigen KW - Cytotoxicity KW - ^g-Interferon KW - Lymphocytes T KW - Tuberculosis KW - Immune response KW - Vaccines KW - Mycobacterium tuberculosis KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17502536?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Effector+Functions+of+Heparin-Binding+Hemagglutinin-Specific+CD8+super%28%2B%29+T+Lymphocytes+in+Latent+Human+Tuberculosis&rft.au=Temmerman%2C+ST%3BPlace%2C+S%3BDebrie%2C+A-S%3BLocht%2C+C%3BMascart%2C+F&rft.aulast=Temmerman&rft.aufirst=ST&rft.date=2005-07-15&rft.volume=192&rft.issue=2&rft.spage=226&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; CD8 antigen; Lymphocytes T; ^g-Interferon; Tuberculosis; Perforin; Hemagglutinins; protective antigen; Memory cells; Vaccines; Immune response; Immunity; Cytotoxicity ER - TY - JOUR T1 - A Potentially Significant Interaction between Efavirenz and Phenytoin: A Case Report and Review of the Literature AN - 17501408; 6386026 AB - Although it has not been demonstrated yet, phenytoin is expected to reduce efavirenz exposure through coinduction of cytochrome P450 (CYP) 3A4 and CYP2B6. Conversely, efavirenz has been shown in vitro to inhibit the enzymes responsible for phenytoin metabolism, CYP2C9 and CYP2C19. We report a case in which a potential bidirectional drug interaction between phenytoin and efavirenz resulted in lower-than-expected efavirenz concentrations and elevated phenytoin levels. Therapeutic drug monitoring was used in this case to ensure adequate efavirenz exposure. JF - Clinical Infectious Diseases AU - Robertson, S M AU - Penzak AU - Lane, J AU - Pau, A K AU - Mican, J M AD - Clinical Pharmacokinetics Research Laboratory, Clinical Center Pharmacy Department, and Office of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/07/15/ PY - 2005 DA - 2005 Jul 15 SP - e15 EP - e18 VL - 41 IS - 2 SN - 1058-4838, 1058-4838 KW - Toxicology Abstracts KW - Drug interaction KW - Phenytoin KW - Efavirenz KW - Case reports KW - Reviews KW - Enzymes KW - Cytochrome P450 KW - Metabolism KW - X 24114:Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17501408?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Infectious+Diseases&rft.atitle=A+Potentially+Significant+Interaction+between+Efavirenz+and+Phenytoin%3A+A+Case+Report+and+Review+of+the+Literature&rft.au=Robertson%2C+S+M%3BPenzak%3BLane%2C+J%3BPau%2C+A+K%3BMican%2C+J+M&rft.aulast=Robertson&rft.aufirst=S&rft.date=2005-07-15&rft.volume=41&rft.issue=2&rft.spage=e15&rft.isbn=&rft.btitle=&rft.title=Clinical+Infectious+Diseases&rft.issn=10584838&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Efavirenz; Phenytoin; Case reports; Cytochrome P450; Metabolism; Reviews; Drug interaction; Enzymes ER - TY - JOUR T1 - Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. AN - 68029576; 16015321 AB - Transposons have provided important genetic tools for functional genomic screens in lower eukaryotes but have proven less useful in higher eukaryotes because of their low transposition frequency. Here we show that Sleeping Beauty (SB), a member of the Tc1/mariner class of transposons, can be mobilized in mouse somatic cells at frequencies high enough to induce embryonic death and cancer in wild-type mice. Tumours are aggressive, with some animals developing two or even three different types of cancer within a few months of birth. The tumours result from SB insertional mutagenesis of cancer genes, thus facilitating the identification of genes and pathways that induce disease. SB transposition can easily be controlled to mutagenize any target tissue and can therefore, in principle, be used to induce many of the cancers affecting humans, including those for which little is known about the aetiology. The uses of SB are also not restricted to the mouse and could potentially be used for forward genetic screens in any higher eukaryote in which transgenesis is possible. JF - Nature AU - Dupuy, Adam J AU - Akagi, Keiko AU - Largaespada, David A AU - Copeland, Neal G AU - Jenkins, Nancy A AD - National Cancer Institute, Center for Cancer Research, Frederick, Maryland 21702, USA. Y1 - 2005/07/14/ PY - 2005 DA - 2005 Jul 14 SP - 221 EP - 226 VL - 436 IS - 7048 KW - DNA Transposable Elements KW - 0 KW - Notch1 protein, mouse KW - Receptor, Notch1 KW - Receptors, Cell Surface KW - Transcription Factors KW - Transposases KW - EC 2.7.7.- KW - Index Medicus KW - Animals KW - Transposases -- metabolism KW - Transcription Factors -- metabolism KW - Mice KW - Embryo, Mammalian -- metabolism KW - Mice, Transgenic KW - Transcription Factors -- genetics KW - Embryo Loss -- genetics KW - Neoplasms -- genetics KW - Receptors, Cell Surface -- metabolism KW - Phenotype KW - Alleles KW - Oncogenes -- genetics KW - Transposases -- genetics KW - Receptors, Cell Surface -- genetics KW - Cell Transformation, Neoplastic -- genetics KW - Mutagenesis, Insertional -- methods KW - DNA Transposable Elements -- genetics KW - Mammals -- genetics KW - Mutagenesis, Insertional -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68029576?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Mammalian+mutagenesis+using+a+highly+mobile+somatic+Sleeping+Beauty+transposon+system.&rft.au=Dupuy%2C+Adam+J%3BAkagi%2C+Keiko%3BLargaespada%2C+David+A%3BCopeland%2C+Neal+G%3BJenkins%2C+Nancy+A&rft.aulast=Dupuy&rft.aufirst=Adam&rft.date=2005-07-14&rft.volume=436&rft.issue=7048&rft.spage=221&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-07-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Nature. 2005 Jul 14;436(7048):184-6 [16015313] Cell. 2005 Aug 12;122(3):322-5 [16096053] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Use of Facs-Assisted Microdissection to isolate Germ-Layer Specific Genes T2 - 4th International European Zebrafish Meeting on Development and Genetics AN - 40107854; 3980735 JF - 4th International European Zebrafish Meeting on Development and Genetics AU - Boorech, J L AU - Kirby, M R AU - Elkahloun, A G AU - Feldman, B Y1 - 2005/07/13/ PY - 2005 DA - 2005 Jul 13 KW - Freshwater fish KW - Genetics KW - Development KW - Danio rerio KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40107854?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.atitle=Use+of+Facs-Assisted+Microdissection+to+isolate+Germ-Layer+Specific+Genes&rft.au=Boorech%2C+J+L%3BKirby%2C+M+R%3BElkahloun%2C+A+G%3BFeldman%2C+B&rft.aulast=Boorech&rft.aufirst=J&rft.date=2005-07-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.issn=&rft_id=info:doi/ L2 - https://www.zebrafish-dresden.com/pub/b/frame.asp?f=2&mid=12&sid=1&m=12 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Comparison of the Mutation Detection Frequency in Reverse Genetic Screens by Tillingand Re-Sequencing Technologies T2 - 4th International European Zebrafish Meeting on Development and Genetics AN - 40105460; 3980784 JF - 4th International European Zebrafish Meeting on Development and Genetics AU - Sood, R AU - Jones, M P AU - Zhang, J AU - Mullikin, J AU - Wu, D Y AU - Wang, D M AU - Yu, J. AU - Liu, P P Y1 - 2005/07/13/ PY - 2005 DA - 2005 Jul 13 KW - Mutation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40105460?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.atitle=Comparison+of+the+Mutation+Detection+Frequency+in+Reverse+Genetic+Screens+by+Tillingand+Re-Sequencing+Technologies&rft.au=Sood%2C+R%3BJones%2C+M+P%3BZhang%2C+J%3BMullikin%2C+J%3BWu%2C+D+Y%3BWang%2C+D+M%3BYu%2C+J.%3BLiu%2C+P+P&rft.aulast=Sood&rft.aufirst=R&rft.date=2005-07-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=4th+International+European+Zebrafish+Meeting+on+Development+and+Genetics&rft.issn=&rft_id=info:doi/ L2 - https://www.zebrafish-dresden.com/pub/b/frame.asp?f=2&mid=12&sid=1&m=12 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Mutation of the adenylylated tyrosine of glutamine synthetase alters its catalytic properties. AN - 68000907; 15996098 AB - Glutamine synthetase is central to nitrogen metabolism in the Gram-negative bacteria. The amount of glutamine synthetase in the cell and its catalytic activity are tightly regulated by multiple, sophisticated mechanisms. Reversible covalent modification of Tyr-397 is central to the regulation of glutamine synthetase activity, via esterification of the hydroxyl group to AMP in a process termed adenylylation. As expected, site-specific mutation of this surface-exposed Tyr-397 to Phe, Ala, or Ser was found to prevent adenylylation. Unexpectedly, these mutations had major effects on the catalytic characteristics of glutamine synthetase. The specific activities of each mutant were approximately doubled, the pH-activity profiles changed, and divalent-cation specificity was altered. Overall, Tyr397Phe behaved as if it were unadenylylated, while both Tyr397Ala and Tyr397Ser behaved as if they were adenylylated. Thus, subtle modifications in the environment of residue 397 are sufficient to induce changes previously thought to require adenylylation. JF - Biochemistry AU - Luo, Shen AU - Kim, Geumsoo AU - Levine, Rodney L AD - Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/07/12/ PY - 2005 DA - 2005 Jul 12 SP - 9441 EP - 9446 VL - 44 IS - 27 SN - 0006-2960, 0006-2960 KW - Cations, Divalent KW - 0 KW - Escherichia coli Proteins KW - Hydroxides KW - Glutamine KW - 0RH81L854J KW - Adenosine Monophosphate KW - 415SHH325A KW - Tyrosine KW - 42HK56048U KW - Manganese KW - 42Z2K6ZL8P KW - Serine KW - 452VLY9402 KW - Phenylalanine KW - 47E5O17Y3R KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - hydroxide ion KW - 9159UV381P KW - gamma-Glutamyltransferase KW - EC 2.3.2.2 KW - Glutamate-Ammonia Ligase KW - EC 6.3.1.2 KW - Magnesium KW - I38ZP9992A KW - Adenine KW - JAC85A2161 KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Cations, Divalent -- metabolism KW - Feedback, Physiological -- genetics KW - Hydrogen-Ion Concentration KW - gamma-Glutamyltransferase -- metabolism KW - Glutamine -- biosynthesis KW - Serine -- genetics KW - Enzyme Activation -- genetics KW - Esterification KW - Manganese -- chemistry KW - Alanine -- genetics KW - Glutamine -- chemistry KW - Phenylalanine -- genetics KW - Hydroxides -- metabolism KW - Magnesium -- chemistry KW - Adenosine Diphosphate -- metabolism KW - Catalysis KW - Mutagenesis, Site-Directed KW - Glutamate-Ammonia Ligase -- genetics KW - Glutamate-Ammonia Ligase -- antagonists & inhibitors KW - Glutamate-Ammonia Ligase -- chemistry KW - Escherichia coli Proteins -- metabolism KW - Glutamate-Ammonia Ligase -- metabolism KW - Adenine -- metabolism KW - Adenosine Monophosphate -- metabolism KW - Tyrosine -- genetics KW - Tyrosine -- metabolism KW - Escherichia coli Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68000907?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Mutation+of+the+adenylylated+tyrosine+of+glutamine+synthetase+alters+its+catalytic+properties.&rft.au=Luo%2C+Shen%3BKim%2C+Geumsoo%3BLevine%2C+Rodney+L&rft.aulast=Luo&rft.aufirst=Shen&rft.date=2005-07-12&rft.volume=44&rft.issue=27&rft.spage=9441&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-15 N1 - Date created - 2005-07-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nonmelanoma skin cancer in relation to ionizing radiation exposure among U.S. radiologic technologists. AN - 67831418; 15704092 AB - Ionizing radiation (IR) is an established cause of nonmelanoma skin cancer, but there is uncertainty about the risk associated with chronic occupational exposure to IR and how it is influenced by ultraviolet radiation (UVR) exposure. We studied 1,355 incident cases with basal cell carcinoma (BCC) and 270 with squamous cell carcinoma (SCC) of the skin in a cohort of 65,304 U.S. white radiologic technologists who responded to the baseline questionnaire survey in 1983-1989 and the follow-up survey in 1994-1998. Cox's proportional-hazards model was used to estimate relative risks of BCC and SCC associated with surrogate measures of occupational exposure to IR and residential UVR exposure during childhood and adulthood, adjusted for potential confounders including pigmentation characteristics. Relative risks of BCC, but not of SCC, were elevated among technologists who first worked during the 1950s (RR = 1.42; 95% CI = 1.12-1.80), 1940s (RR = 2.04; 95% CI = 1.44-2.88) and before 1940 (RR = 2.16; 95% CI = 1.14-4.09), when IR exposures were high, compared to those who first worked after 1960 (p for trend < 0.01). The effect of year first worked on BCC risk was not modified by UVR exposure, but was significantly stronger among individuals with lighter compared to darker eye and hair color (p = 0.013 and 0.027, respectively). This study provides some evidence that chronic occupational exposure to IR at low to moderate levels can increase the risk of BCC, and that this risk may be modified by pigmentation characteristics. (c) 2005 Wiley-Liss, Inc. JF - International journal of cancer AU - Yoshinaga, Shinji AU - Hauptmann, Michael AU - Sigurdson, Alice J AU - Doody, Michele Morin AU - Freedman, D Michal AU - Alexander, Bruce H AU - Linet, Martha S AU - Ron, Elaine AU - Mabuchi, Kiyohiko AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA. yosinaga@nirs.go.jp Y1 - 2005/07/10/ PY - 2005 DA - 2005 Jul 10 SP - 828 EP - 834 VL - 115 IS - 5 SN - 0020-7136, 0020-7136 KW - Index Medicus KW - Allied Health Personnel KW - Risk Factors KW - Humans KW - Cohort Studies KW - Adult KW - Aged KW - Middle Aged KW - Skin Pigmentation KW - Male KW - Female KW - Radiation, Ionizing KW - Occupational Exposure KW - Carcinoma, Squamous Cell -- etiology KW - Carcinoma, Squamous Cell -- epidemiology KW - Carcinoma, Basal Cell -- etiology KW - Skin Neoplasms -- etiology KW - Radiology -- manpower KW - Skin Neoplasms -- epidemiology KW - Radiation Injuries -- epidemiology KW - Carcinoma, Basal Cell -- epidemiology KW - Radiation Injuries -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67831418?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+cancer&rft.atitle=Nonmelanoma+skin+cancer+in+relation+to+ionizing+radiation+exposure+among+U.S.+radiologic+technologists.&rft.au=Yoshinaga%2C+Shinji%3BHauptmann%2C+Michael%3BSigurdson%2C+Alice+J%3BDoody%2C+Michele+Morin%3BFreedman%2C+D+Michal%3BAlexander%2C+Bruce+H%3BLinet%2C+Martha+S%3BRon%2C+Elaine%3BMabuchi%2C+Kiyohiko&rft.aulast=Yoshinaga&rft.aufirst=Shinji&rft.date=2005-07-10&rft.volume=115&rft.issue=5&rft.spage=828&rft.isbn=&rft.btitle=&rft.title=International+journal+of+cancer&rft.issn=00207136&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-29 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protein kinase C phosphorylation of the metabotropic glutamate receptor mGluR5 on Serine 839 regulates Ca2+ oscillations. AN - 67997857; 15894802 AB - The activation of Group 1 metabotropic glutamate receptors, mGluR5 and mGluR1alpha, triggers intracellular calcium release; however, mGluR5 activation is unique in that it elicits Ca2+ oscillations. A short region of the mGluR5 C terminus is the critical determinant and differs from the analogous region of mGluR1alpha by a single amino acid residue, Thr-840, which is an aspartic acid (Asp-854) in mGluR1alpha. Previous studies show that mGluR5-elicited Ca2+ oscillations require protein kinase C (PKC)-dependent phosphorylation and identify Thr-840 as the phosphorylation site. However, direct phosphorylation of mGluR5 has not been studied in detail. We have used biochemical analyses to directly investigate the phosphorylation of the mGluR5 C terminus. We showed that Ser-839 on mGluR5 is directly phosphorylated by PKC, whereas Thr-840 plays a permissive role. Although Ser-839 is conserved in mGluR1alpha (Ser-853), it is not phosphorylated, as the adjacent residue (Asp-854) is not permissive; however, mutagenesis of Asp-854 to a permissive alanine residue allows phosphorylation of Ser-853 on mGluR1alpha. We investigated the physiological consequences of mGluR5 Ser-839 phosphorylation using Ca2+ imaging. Mutations that eliminate Ser-839 phosphorylation prevent the characteristic mGluR5-dependent Ca2+ oscillations. However, mutation of Thr-840 to alanine, which prevents potential Thr-840 phosphorylation but is still permissive for Ser-839 phosphorylation, has no effect on Ca2+ oscillations. Thus, we showed that it is phosphorylation of Ser-839, not Thr-840, that is absolutely required for the unique Ca2+ oscillations produced by mGluR5 activation. The Thr-840 residue is important only in that it is permissive for the PKC-dependent phosphorylation of Ser-839. JF - The Journal of biological chemistry AU - Kim, Chul Hoon AU - Braud, Stephanie AU - Isaac, John T R AU - Roche, Katherine W AD - NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07/08/ PY - 2005 DA - 2005 Jul 08 SP - 25409 EP - 25415 VL - 280 IS - 27 SN - 0021-9258, 0021-9258 KW - Antibodies KW - 0 KW - GRM5 protein, human KW - Receptor, Metabotropic Glutamate 5 KW - Receptors, Metabotropic Glutamate KW - Serine KW - 452VLY9402 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Animals KW - Phosphorylation KW - HeLa Cells KW - Humans KW - Molecular Sequence Data KW - Rabbits KW - Amino Acid Sequence KW - Serine -- metabolism KW - Serine -- immunology KW - Protein Kinase C -- metabolism KW - Calcium Signaling -- physiology KW - Receptors, Metabotropic Glutamate -- genetics KW - Receptors, Metabotropic Glutamate -- metabolism KW - Receptors, Metabotropic Glutamate -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67997857?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C+phosphorylation+of+the+metabotropic+glutamate+receptor+mGluR5+on+Serine+839+regulates+Ca2%2B+oscillations.&rft.au=Kim%2C+Chul+Hoon%3BBraud%2C+Stephanie%3BIsaac%2C+John+T+R%3BRoche%2C+Katherine+W&rft.aulast=Kim&rft.aufirst=Chul&rft.date=2005-07-08&rft.volume=280&rft.issue=27&rft.spage=25409&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Thionylation of PKC by Reactive Sulfur Species: Disulfide S-Monoxide and Disulfide S-Dioxide T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40041156; 3969310 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Huang, F L AU - Huang, K.-P. Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Protein kinase C KW - Sulfur KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40041156?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Thionylation+of+PKC+by+Reactive+Sulfur+Species%3A+Disulfide+S-Monoxide+and+Disulfide+S-Dioxide&rft.au=Huang%2C+F+L%3BHuang%2C+K.-P.&rft.aulast=Huang&rft.aufirst=F&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Drosophila Myosin V: Solution Kinetics and Motile Properties T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40013825; 3969057 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Toth, J AU - Kovacs, M AU - Wang, F AU - Nyitray, L AU - Sellers, J R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Kinetics KW - Myosin KW - Drosophila KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40013825?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Drosophila+Myosin+V%3A+Solution+Kinetics+and+Motile+Properties&rft.au=Toth%2C+J%3BKovacs%2C+M%3BWang%2C+F%3BNyitray%2C+L%3BSellers%2C+J+R&rft.aulast=Toth&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - SC35 Promotes Prolonged Stress- Induced 3Alternative Splicing of Acetylcholinesterase T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40013449; 3968169 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Meshorer, E AU - Toiber, D AU - Bryk, B AU - Dori, A AU - Soreq, H Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Acetylcholinesterase KW - Splicing KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40013449?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=SC35+Promotes+Prolonged+Stress-+Induced+3Alternative+Splicing+of+Acetylcholinesterase&rft.au=Meshorer%2C+E%3BToiber%2C+D%3BBryk%2C+B%3BDori%2C+A%3BSoreq%2C+H&rft.aulast=Meshorer&rft.aufirst=E&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Phosphorylation and O-Glycosylation Sites Identification in Peptides by Ba-Hydroxide Catalyzed b-Elimination/Propanethiol Addition and Mass Spectrometric Analyses T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40008412; 3969653 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Chen, H.-C. AU - Wang, G AU - Backlund, P S AU - Boykins, R A Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Phosphorylation KW - Peptides KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40008412?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Phosphorylation+and+O-Glycosylation+Sites+Identification+in+Peptides+by+Ba-Hydroxide+Catalyzed+b-Elimination%2FPropanethiol+Addition+and+Mass+Spectrometric+Analyses&rft.au=Chen%2C+H.-C.%3BWang%2C+G%3BBacklund%2C+P+S%3BBoykins%2C+R+A&rft.aulast=Chen&rft.aufirst=H.-C.&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - New ABC Transporters Associated with Multidrug Resistance in Cancer T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40003226; 3968650 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Gottesman, M M AU - Paterson, J K AU - Chen, K G AU - Annereau, J P AU - Szakacs, G Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - ABC transporter KW - Multidrug resistance KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40003226?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=New+ABC+Transporters+Associated+with+Multidrug+Resistance+in+Cancer&rft.au=Gottesman%2C+M+M%3BPaterson%2C+J+K%3BChen%2C+K+G%3BAnnereau%2C+J+P%3BSzakacs%2C+G&rft.aulast=Gottesman&rft.aufirst=M&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - ABCB6 Overexpression is Associated with Resistance to Arsenite, Cisplatin, and Antimony T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 40002442; 3969912 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Paterson, J K AU - Tachiwada, T AU - Szakacs, G AU - Akiyama, S.-i. AU - Gottesman, M M Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Cisplatin KW - Antimony KW - Arsenite KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40002442?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=ABCB6+Overexpression+is+Associated+with+Resistance+to+Arsenite%2C+Cisplatin%2C+and+Antimony&rft.au=Paterson%2C+J+K%3BTachiwada%2C+T%3BSzakacs%2C+G%3BAkiyama%2C+S.-i.%3BGottesman%2C+M+M&rft.aulast=Paterson&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Regulation and Mechanics of Myosin V T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39998435; 3969047 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Sellers, J R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Myosin KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39998435?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Regulation+and+Mechanics+of+Myosin+V&rft.au=Sellers%2C+J+R&rft.aulast=Sellers&rft.aufirst=J&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Structure and Expression of p53 Gene and Altered Phosphorylation of p53 Protein in Human Vestibular Schwannomas T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39998041; 3968120 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Prabhu, P. Antony Herold AU - Mathivanan, J AU - Rohini, K AU - Thomas, R AU - Chandramouli, B AU - Gope, R Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - Schwann cells KW - Phosphorylation KW - Vestibular system KW - p53 protein KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39998041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Structure+and+Expression+of+p53+Gene+and+Altered+Phosphorylation+of+p53+Protein+in+Human+Vestibular+Schwannomas&rft.au=Prabhu%2C+P.+Antony+Herold%3BMathivanan%2C+J%3BRohini%2C+K%3BThomas%2C+R%3BChandramouli%2C+B%3BGope%2C+R&rft.aulast=Prabhu&rft.aufirst=P.+Antony&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Arrested Apoptosis in Lens Fiber Cells: A Possible Role of a-Crystallin T2 - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AN - 39996834; 3968433 JF - 30th FEBS Congress and the 9th IUBMB Conference on the Protein World AU - Morozov, V AU - Wawrousek, E F Y1 - 2005/07/02/ PY - 2005 DA - 2005 Jul 02 KW - crystallin KW - Apoptosis KW - Eye lens KW - Fibers KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39996834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.atitle=Arrested+Apoptosis+in+Lens+Fiber+Cells%3A+A+Possible+Role+of+a-Crystallin&rft.au=Morozov%2C+V%3BWawrousek%2C+E+F&rft.aulast=Morozov&rft.aufirst=V&rft.date=2005-07-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=30th+FEBS+Congress+and+the+9th+IUBMB+Conference+on+the+Protein+World&rft.issn=&rft_id=info:doi/ L2 - http://www.febs-iubmb-2005.com/symposia.php?phase=1&d_m=on&d_t=on&d_a=on&d_ o=on&d_s=on&d_p=on&d_d=on&d_c=on&d_k=on&email=n LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Fanconi anemia: adult head and neck cancer and hematopoietic mosaicism. AN - 85387542; pmid-16027289 JF - Archives of otolaryngology--head & neck surgery AU - Alter, Blanche P AU - Joenje, Hans AU - Oostra, Anneke B AU - Pals, Gerard AD - Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Bethesda, MD, USA. alterb@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 635 EP - 639 VL - 131 IS - 7 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - Adult KW - *Carcinoma, Squamous Cell: etiology KW - Carcinoma, Squamous Cell: genetics KW - Chromosome Breakage KW - *Fanconi Anemia: complications KW - Fanconi Anemia: genetics KW - Female KW - *Head and Neck Neoplasms: etiology KW - Head and Neck Neoplasms: genetics KW - Humans KW - Male KW - Middle Aged KW - *Mosaicism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85387542?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Fanconi+anemia%3A+adult+head+and+neck+cancer+and+hematopoietic+mosaicism.&rft.au=Alter%2C+Blanche+P%3BJoenje%2C+Hans%3BOostra%2C+Anneke+B%3BPals%2C+Gerard&rft.aulast=Alter&rft.aufirst=Blanche&rft.date=2005-07-01&rft.volume=131&rft.issue=7&rft.spage=635&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - SuppNotes - Comment In: Arch Otolaryngol Head Neck Surg. 2005 Jul;131(7):640-1[16027290] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Head and neck squamous cell carcinoma in patients with Fanconi anemia. AN - 85383731; pmid-16027290 JF - Archives of otolaryngology--head & neck surgery AU - Van Waes, Carter AD - Head and Neck Surgery Branch, National Institute on Deafness and Other Communication Disorders, Bethesda, MD 20892-1462, USA. vanwaesc@nidcd.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 640 EP - 641 VL - 131 IS - 7 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - *Carcinoma, Squamous Cell: etiology KW - Carcinoma, Squamous Cell: genetics KW - *Fanconi Anemia: complications KW - Fanconi Anemia: genetics KW - *Head and Neck Neoplasms: etiology KW - Head and Neck Neoplasms: genetics KW - Humans UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85383731?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Head+and+neck+squamous+cell+carcinoma+in+patients+with+Fanconi+anemia.&rft.au=Van+Waes%2C+Carter&rft.aulast=Van+Waes&rft.aufirst=Carter&rft.date=2005-07-01&rft.volume=131&rft.issue=7&rft.spage=640&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - SuppNotes - Comment On: Arch Otolaryngol Head Neck Surg. 2005 Jul;131(7):635-9[16027289] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Mutation of the Parkin gene in a Persian family: clinical progression over a 40-year period. AN - 85381972; pmid-15852366 AB - We report on an Israeli family originating from Iran in which 4 of 7 brothers born from a consanguineous marriage had juvenile Parkinsonism. Linkage analysis of markers covering the autosomal recessive juvenile Parkinsonism (AR-JP, PARK2, Parkin gene, OMIM #602544) gene resulted in a maximal logarithm of odds score of 2.18. A homozygous deletion that expanded from exon 4 to exon 6 was identified in all the patients. Significant clinical heterogeneity was present between siblings.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Clarimon, Jordi AU - Johnson, Janel AU - Djaldetti, Ruth AU - Hernandez, Dena AU - Hattori, Nobutaka AU - Sroka, Hava AU - Barhom, Yael AU - Singleton, Andrew AD - Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, USA. clarimon@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 887 EP - 890 VL - 20 IS - 7 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Aged KW - DNA Mutational Analysis: methods KW - Exons KW - Female KW - Humans KW - Iran KW - Male KW - *Mutation KW - Odds Ratio KW - *Parkinsonian Disorders: genetics KW - Pedigree KW - RNA, Messenger: biosynthesis KW - Reverse Transcriptase Polymerase Chain Reaction: methods KW - *Siblings KW - *Ubiquitin-Protein Ligases: genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85381972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Mutation+of+the+Parkin+gene+in+a+Persian+family%3A+clinical+progression+over+a+40-year+period.&rft.au=Clarimon%2C+Jordi%3BJohnson%2C+Janel%3BDjaldetti%2C+Ruth%3BHernandez%2C+Dena%3BHattori%2C+Nobutaka%3BSroka%2C+Hava%3BBarhom%2C+Yael%3BSingleton%2C+Andrew&rft.aulast=Clarimon&rft.aufirst=Jordi&rft.date=2005-07-01&rft.volume=20&rft.issue=7&rft.spage=887&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Polymeric Scaffolds for Cartilage Tissue Engineering AN - 754562450; 13386477 AB - Polymeric scaffolds are three-dimensional, porous structures that may be used as a vehicle to deliver cells or therapeutic factors to repair tissue defects. Both biodegradable and non-biodegradable polymers have been developed for this purpose. In this review, we survey the polymers that have been investigated for cartilage tissue engineering and discuss the critical parameters for successful applications in the future. JF - Macromolecular Symposia AU - Li, Wan-Ju AU - Tuan, Rocky S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA,, Tuanr@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 65 EP - 76 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 227 IS - 1 SN - 1022-1360, 1022-1360 KW - Biotechnology and Bioengineering Abstracts; Calcium & Calcified Tissue Abstracts KW - Macromolecules KW - Cartilage KW - Tissue engineering KW - Biodegradability KW - scaffolds KW - T 2030:Cartilage and Cartilage Diseases KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/754562450?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Macromolecular+Symposia&rft.atitle=Polymeric+Scaffolds+for+Cartilage+Tissue+Engineering&rft.au=Li%2C+Wan-Ju%3BTuan%2C+Rocky+S&rft.aulast=Li&rft.aufirst=Wan-Ju&rft.date=2005-07-01&rft.volume=227&rft.issue=1&rft.spage=65&rft.isbn=&rft.btitle=&rft.title=Macromolecular+Symposia&rft.issn=10221360&rft_id=info:doi/10.1002%2Fmasy.200550906 L2 - http://www3.interscience.wiley.com/journal/110576993/abstract LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-08-01 N1 - Last updated - 2015-03-19 N1 - SubjectsTermNotLitGenreText - Macromolecules; Cartilage; Tissue engineering; Biodegradability; scaffolds DO - http://dx.doi.org/10.1002/masy.200550906 ER - TY - JOUR T1 - Cancer biology and hormesis: comments on Calabrese (2005). AN - 69072980; 16422393 AB - Large numbers of chemicals of a variety of types exhibit apparent hormetic effects on cultured human cancer cells, causing stimulation of cell growth or related changes at low doses, followed by inhibition at higher doses. Many of the studies listed are not fully convincing, due to lack of appropriate controls or sufficient number of doses. However, the proposed hormetic response seems firmly established in a subset of these experiments. Significance with regard to in vivo cancer growth has not been pursued and must be a priority for the future. For several examples where in vivo titers are known, such as, resveratrol, suramin, and tamoxifen, comparison of the dose and concentration ranges confirms that hormesis could be an issue in vivo. Further investigations are warranted, especially for therapeutic drugs, phytochemicals, and environmental toxicants. JF - Critical reviews in toxicology AU - Anderson, Lucy M AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, Ft Detrick, Maryland 21702, USA. Andersol@ncifcrf.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 583 EP - 586 VL - 35 IS - 6 SN - 1040-8444, 1040-8444 KW - Antineoplastic Agents KW - 0 KW - Carcinogens KW - Stilbenes KW - Tamoxifen KW - 094ZI81Y45 KW - Suramin KW - 6032D45BEM KW - resveratrol KW - Q369O8926L KW - Index Medicus KW - Tamoxifen -- pharmacology KW - No-Observed-Adverse-Effect Level KW - Tumor Cells, Cultured KW - Stilbenes -- pharmacology KW - Dose-Response Relationship, Drug KW - Humans KW - Cell Line, Tumor KW - Suramin -- pharmacology KW - Risk Assessment KW - Adaptation, Physiological -- drug effects KW - Adaptation, Physiological -- physiology KW - Cell Division -- drug effects KW - Carcinogens -- toxicity KW - Antineoplastic Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/69072980?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Critical+reviews+in+toxicology&rft.atitle=Cancer+biology+and+hormesis%3A+comments+on+Calabrese+%282005%29.&rft.au=Anderson%2C+Lucy+M&rft.aulast=Anderson&rft.aufirst=Lucy&rft.date=2005-07-01&rft.volume=35&rft.issue=6&rft.spage=583&rft.isbn=&rft.btitle=&rft.title=Critical+reviews+in+toxicology&rft.issn=10408444&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-02 N1 - Date created - 2006-01-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment On: Crit Rev Toxicol. 2005 Jul;35(6):463-582 [16422392] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Medical management of HIV-hepatitis C virus coinfection in injection drug users. AN - 68752312; 16265605 AB - Several million people inject drugs of abuse and, as a result, are coinfected with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). The treatment of this coinfected drug-abusing population is fraught with many problems such that clinicians and other health care providers have to determine whether patients should be treated first for drug addiction, for HIV/AIDS, or for HCV infection or simultaneously treated. These proceedings present the incidence and prevalence of coinfections with HIV and HCV in high-risk populations and discuss the underlying pathophysiology of coinfections and the problems and strategies of managing the treatment of coinfections among people who also inject illicit drugs. In addition, the expert panel recommended further research to determine the best possible treatment regimens applicable to injection drug users coinfected with HIV and HCV. JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Khalsa, Jag H AU - Kresina, Tom AU - Sherman, Ken AU - Vocci, Francis AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, National Institute on Drug Abuse, National Institutes of Health, Bethesda, Maryland 20892-9593, USA. jk98p@nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - S1 EP - S6 VL - 41 Suppl 1 KW - Index Medicus KW - Humans KW - Incidence KW - Prevalence KW - Hepatitis C -- drug therapy KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - Disease Management KW - HIV Infections -- drug therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- epidemiology KW - Hepatitis C -- epidemiology KW - Substance Abuse, Intravenous -- complications KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68752312?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Medical+management+of+HIV-hepatitis+C+virus+coinfection+in+injection+drug+users.&rft.au=Khalsa%2C+Jag+H%3BKresina%2C+Tom%3BSherman%2C+Ken%3BVocci%2C+Francis&rft.aulast=Khalsa&rft.aufirst=Jag&rft.date=2005-07-01&rft.volume=41+Suppl+1&rft.issue=&rft.spage=S1&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Integrating care for hepatitis C virus (HCV) and primary care for HIV for injection drug users coinfected with HIV and HCV. AN - 68751300; 16265621 AB - Injection drug use accounts for most of the incident infections with hepatitis C virus (HCV) and for at least one-third of new human immunodeficiency virus (HIV) infections. Coinfection with HCV and HIV presents complex and challenging medical conditions. Ensuring access to and maintaining care for HIV and HCV for drug users presents special challenges to the health care team that require a nonjudgmental attitude, experience, and patience. Care for HCV infection, however, can be used as an instrument to engage drug-using persons in ongoing primary care relationships. Common elements to both care for HCV infection and primary care for HIV infection are testing for and counseling about HCV and HIV, substance abuse and mental health services, social support, and subspecialty referral. These elements, in particular treatment for substance abuse, can be focal points for model care systems that provide integrative care for both HCV and HIV infections. JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Kresina, Thomas F AU - Bruce, R Douglas AU - Cargill, Victoria A AU - Cheever, Laura W AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, National Institute on Drug Abuse, Bethesda, MD 20892, USA. tk13v@nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - S83 EP - S88 VL - 41 Suppl 1 KW - Index Medicus KW - Substance Abuse Treatment Centers -- organization & administration KW - Humans KW - Professional-Patient Relations KW - Primary Health Care -- organization & administration KW - Male KW - Female KW - Hepatitis C -- therapy KW - HIV Infections -- complications KW - Hepatitis C -- complications KW - HIV Infections -- therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- complications KW - Delivery of Health Care, Integrated UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68751300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Integrating+care+for+hepatitis+C+virus+%28HCV%29+and+primary+care+for+HIV+for+injection+drug+users+coinfected+with+HIV+and+HCV.&rft.au=Kresina%2C+Thomas+F%3BBruce%2C+R+Douglas%3BCargill%2C+Victoria+A%3BCheever%2C+Laura+W&rft.aulast=Kresina&rft.aufirst=Thomas&rft.date=2005-07-01&rft.volume=41+Suppl+1&rft.issue=&rft.spage=S83&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - No correlation between radiosensitivity or double-strand break repair capacity of normal fibroblasts and acute normal tissue reaction after radiotherapy of breast cancer patients. AN - 68750475; 16263653 AB - The aim was to study the relationship between cellular radiosensitivity or double-strand break (dsb) repair capacity of skin fibroblasts and the extent of acute reaction after radiotherapy for breast cancer. The study was performed with 25 breast cancer patients submitted to the radiotherapy unit of the Egyptian National Cancer Institute after conserving surgery. Dermal fibroblasts, established from skin biopsies, were used to determine the cellular radiosensitivity via colony assay and the capacity of dsb repair by constant-field gel electrophoresis. Acute reactions were scored using the Radiation Therapy Oncology Group (RTOG) classification. The spectrum of acute reactions varied from grade 1 to 4, whereby most patients developed a grade 1 reaction after total doses ranging between 46 and 70 Gy. Skin fibroblasts showed a pronounced variation in both cellular radiosensitivity expressed as the mean inactivation dose (Dbar) (coefficient of variation, CV=25%) as well as in the number of residual dsb (CV=33%) with no significant correlation between these two endpoints (r2=0.20, p=0.14). Both parameters did not correlate with the extent of acute reaction of the respective patient. The data obtained indicate that the sensitivity of fibroblasts measured either by colony assay or by dsb repair capacity is not a major parameter determining the extent of acute reaction after radiotherapy of breast cancer patients. JF - International journal of radiation biology AU - El-Awady, R A AU - Mahmoud, M AU - Saleh, E M AU - El-Baky, H Abd AU - Lotayef, M AU - Dahm-Daphi, J AU - Dikomey, E AD - Tumour Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt, and Department of Radiotherapy and Radiation Oncology, University-Hospital Hamburg-Eppendorf, Hamburg, Germany. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 501 EP - 508 VL - 81 IS - 7 SN - 0955-3002, 0955-3002 KW - Index Medicus KW - Space life sciences KW - Severity of Illness Index KW - Skin -- radiation effects KW - Humans KW - Adult KW - Skin -- cytology KW - Radiation Tolerance KW - Middle Aged KW - Biopsy KW - Female KW - Fibroblasts KW - DNA Repair KW - DNA Damage KW - Radiation Injuries -- classification KW - Breast Neoplasms -- radiotherapy KW - Radiation Injuries -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68750475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+biology&rft.atitle=No+correlation+between+radiosensitivity+or+double-strand+break+repair+capacity+of+normal+fibroblasts+and+acute+normal+tissue+reaction+after+radiotherapy+of+breast+cancer+patients.&rft.au=El-Awady%2C+R+A%3BMahmoud%2C+M%3BSaleh%2C+E+M%3BEl-Baky%2C+H+Abd%3BLotayef%2C+M%3BDahm-Daphi%2C+J%3BDikomey%2C+E&rft.aulast=El-Awady&rft.aufirst=R&rft.date=2005-07-01&rft.volume=81&rft.issue=7&rft.spage=501&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+biology&rft.issn=09553002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-13 N1 - Date created - 2005-11-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Anticarcinogenic effects of an aqueous infusion of cloves on skin carcinogenesis. AN - 68708457; 16235990 AB - Spices and flavouring agents are now receiving increasing attention as many of them have been shown to have anticarcinogenic properties. Cloves, sun-dried unopened flower buds from the plant Syzygium aromaticum L, are commonly used as a spice and food flavour. The present study was designed to investigate the chemopreventive action of aqueous infusion of cloves on 9,10-dimethyl benz(a)anthracene (DMBA) and croton oil induced skin carcinogenesis in Swiss mice. The results indicate protection against skin papilloma formation in a dose dependent manner. It has been shown that oral administration of aqueous infusions of clove at a dose of 100 microl/mouse/day not only delays the formation of papilloma but also reduces the incidence of papilloma as well as the cumulative number of papillomas per papilloma bearing mouse. Our observations suggest a promising role for cloves in restriction of the carcinogenesis process. JF - Asian Pacific journal of cancer prevention : APJCP AU - Banerjee, Sarmistha AU - Das, Sukta AD - Dept. of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700026, West Bengal, India. rmisti@rediffmail.com PY - 2005 SP - 304 EP - 308 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Carcinogens KW - 0 KW - Plant Extracts KW - 9,10-Dimethyl-1,2-benzanthracene KW - 57-97-6 KW - Index Medicus KW - Administration, Oral KW - Animals KW - Mice KW - Neoplasms, Experimental KW - Skin Neoplasms -- physiopathology KW - Plant Extracts -- pharmacology KW - Papilloma -- prevention & control KW - Papilloma -- physiopathology KW - Cell Transformation, Neoplastic -- drug effects KW - Chemoprevention KW - Skin Neoplasms -- prevention & control KW - Syzygium -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68708457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Anticarcinogenic+effects+of+an+aqueous+infusion+of+cloves+on+skin+carcinogenesis.&rft.au=Banerjee%2C+Sarmistha%3BDas%2C+Sukta&rft.aulast=Banerjee&rft.aufirst=Sarmistha&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=304&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Glutathione S-transferase M1 gene polymorphism in Thai nasopharyngeal carcinoma. AN - 68704152; 16235985 AB - Nasopharyngeal carcinoma (NPC) is a serious health problem in Thailand. It is caused by the combined effects of Epstein-Barr virus (EBV), carcinogens and genetic susceptibility. The glutathione S-transferase M1 gene (GSTM1) encodes a phase II enzyme responsible for detoxifying carcinogenic electrophiles. Polymorphic null forms of the gene GSTM1 lack enzyme activity and have been associated with susceptibility to several cancers including NPC. To examine the association between GSTM1 polymorphism and NPC susceptibility in Thais, GSTM1 genotypes (normal and null genotypes) in 78 NPC patients and 145 age-matched healthy controls were determined using PCR assays. Overall, no statistically significant differences were observed in the frequency of GSTM1 genotypes between cases and controls, nor among NPC patients compared on the basis of sex and clinical stage of disease. Carriers with the GSTM1 null genotype had a 2.9-fold increased risk for NPC of WHO type III when compared to those with GSTM1 normal genotype (P 40, (>45 and (>50 years), the frequencies of GSTM1 null genotype in cases the (>45 and (>50 age groups were significantly different from controls (P45 and (>50 years had a 2-fold and 3-fold increased risk for NPC when compared to those with GSTM1 normal genotype (OR = 2.2, 95% CI = 1.1-4.7 and OR = 3.0, 95% CI = 1.2-7.5). We suggest that GSTM1 polymorphism may be associated with NPC susceptibility in Thais, especially for GSTM1 null genotype carriers of age higher than 45 years. The GSTM1 null genotype may be a useful genetic marker for predicting Thai NPC and for screening of early stages of Thai NPC. JF - Asian Pacific journal of cancer prevention : APJCP AU - Tiwawech, Danai AU - Srivatanakul, Petcharin AU - Karalak, Anant AU - Ishida, Takafumi AD - Research Division, National Cancer Institute, Bangkok 10400, Thailand. tdanai@hotmail.com PY - 2005 SP - 270 EP - 275 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Glutathione Transferase KW - EC 2.5.1.18 KW - glutathione S-transferase M1 KW - Index Medicus KW - Genotype KW - Polymerase Chain Reaction KW - Aged, 80 and over KW - Humans KW - Adult KW - Case-Control Studies KW - Aged KW - Thailand -- epidemiology KW - Middle Aged KW - Male KW - Female KW - Polymorphism, Genetic KW - Nasopharyngeal Neoplasms -- epidemiology KW - Glutathione Transferase -- genetics KW - Genetic Predisposition to Disease KW - Nasopharyngeal Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68704152?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Glutathione+S-transferase+M1+gene+polymorphism+in+Thai+nasopharyngeal+carcinoma.&rft.au=Tiwawech%2C+Danai%3BSrivatanakul%2C+Petcharin%3BKaralak%2C+Anant%3BIshida%2C+Takafumi&rft.aulast=Tiwawech&rft.aufirst=Danai&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=270&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of growth, induction of apoptosis and alteration of gene expression by tea polyphenols in the highly metastatic human lung cancer cell line NCI-H460. AN - 68702286; 16235994 AB - Lung cancer is a complex group of diseases but each lesion is thought to originate from a single mutated progenitor cell. It is evident that multiple genetic changes are involved in the generation of each specific type of lung cancer. Due to the high complexity of these processes and rapid metastasis, treatment of advanced lung cancer, particularly of NSCLCs, is far from satisfactory. Thus, there is a need for innovative strategies for modulation of adverse alteration in protooncogene or tumor suppressor genes so that lung carcinogenesis can be suppressed or delayed. To this end, we have evaluated the effects of tea compounds (theaflavins, epicatechin-gallate and epigallo-catechin-gallate) on proliferation and apoptosis and associated gene expression in a highly metastatic human lung cancer cell line NCI-H460. Significant reduction of cell proliferation, detected in situ by BrdU incorporation, and induction of apoptosis, assessed by the by the TUNEL method, were noted following treatments. Expression of p53, Bcl-2, c-Myc and H-Ras, was localized by immunocytochemistry and analysed by Western blotting. Tea compounds upregulated expression of p53, downregulated expression of Bcl-2 but there was no significant influence on H-ras and c-Myc expressions. It is suggested that tea compounds can influence genetic alteration to disfavour, growth and survival of lung cancer cells. JF - Asian Pacific journal of cancer prevention : APJCP AU - Ganguly, Chaiti AU - Saha, Prosenjit AU - Panda, Chinmay Kr AU - Das, Sukta AD - Dept. of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37 S.P. Mukherjee Road, Kolkata 700026, India. PY - 2005 SP - 326 EP - 331 VL - 6 IS - 3 SN - 1513-7368, 1513-7368 KW - Flavonoids KW - 0 KW - Phenols KW - Polyphenols KW - Tea KW - Index Medicus KW - Cell Proliferation -- drug effects KW - In Situ Nick-End Labeling KW - Genes, bcl-2 KW - Blotting, Western KW - Tumor Cells, Cultured KW - Genes, p53 KW - Down-Regulation KW - Humans KW - Apoptosis -- drug effects KW - Immunohistochemistry KW - Phenols -- pharmacology KW - Neoplasm Metastasis KW - Flavonoids -- pharmacology KW - Lung Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68702286?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.atitle=Inhibition+of+growth%2C+induction+of+apoptosis+and+alteration+of+gene+expression+by+tea+polyphenols+in+the+highly+metastatic+human+lung+cancer+cell+line+NCI-H460.&rft.au=Ganguly%2C+Chaiti%3BSaha%2C+Prosenjit%3BPanda%2C+Chinmay+Kr%3BDas%2C+Sukta&rft.aulast=Ganguly&rft.aufirst=Chaiti&rft.date=2005-07-01&rft.volume=6&rft.issue=3&rft.spage=326&rft.isbn=&rft.btitle=&rft.title=Asian+Pacific+journal+of+cancer+prevention+%3A+APJCP&rft.issn=15137368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-10-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Photoactivation of quantum dot fluorescence following endocytosis. AN - 68616810; 16178255 AB - We studied the fluorescence of quantum dots in cells. Coating quantum dots with cationic peptides caused them to be endocytosed and transported to lysosomes. After overnight incubation, their fluorescence apparently dimmed but became markedly "photoactivatable", increasing more than 3-fold within minutes on exposure to bright light, and decaying over hours in the dark. Photoactivation was greater in the presence of water than ethanol, and UV illumination compensated for lack of water during photoactivation. Dimming and photoactivation could affect the use of quantum dots as quantitative probes in vivo and lead to new uses, such as tracking molecular movement. JF - Nano letters AU - Silver, Jonathan AU - Ou, Wu AD - Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. jsilver@nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1445 EP - 1449 VL - 5 IS - 7 SN - 1530-6984, 1530-6984 KW - Fluorescent Dyes KW - 0 KW - Index Medicus KW - Radiation Dosage KW - Animals KW - HeLa Cells KW - Humans KW - Light KW - Dose-Response Relationship, Radiation KW - Cell Line KW - Cricetinae KW - Luminescent Measurements -- methods KW - Kidney -- radiation effects KW - Endosomes -- physiology KW - Photochemistry -- methods KW - Quantum Dots KW - Kidney -- physiology KW - Microscopy, Fluorescence -- methods KW - Endocytosis -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68616810?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nano+letters&rft.atitle=Photoactivation+of+quantum+dot+fluorescence+following+endocytosis.&rft.au=Silver%2C+Jonathan%3BOu%2C+Wu&rft.aulast=Silver&rft.aufirst=Jonathan&rft.date=2005-07-01&rft.volume=5&rft.issue=7&rft.spage=1445&rft.isbn=&rft.btitle=&rft.title=Nano+letters&rft.issn=15306984&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-09-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic instability: the dark side of the hypoxic response. AN - 68515597; 15970707 AB - Under low oxygen tension, the activated transcription factor HIF-1alpha upregulates an array of hypoxia-inducible genes via heterodimerization with ARNT and binding to the hypoxia-responsive element in the promoter. Alternatively, HIF-1alpha regulates hypoxia-responsive genes by functionally antagonizing the oncoprotein Myc via protein-protein interactions. This so-called HIF-1alpha-Myc mechanism apparently not only accounts for the gene upregulation, but also for the gene downregulation during hypoxia, depending upon the activating and repressive nature of Myc in gene expression. Indeed, our recent study demonstrated that both mismatch repair genes, MSH2 and MSH6, are inhibited by this mechanism in a p53-dependent manner. In particular, the constitutively bound transcription factor Sp1 serves as a molecular switch by recruiting HIF-1alpha in hypoxia to displace the transcription activator Myc from the promoter. Therefore, our findings shed light on the mechanisms underlying hypoxia-induced genetic instability, an "adverse"effect of the hypoxic response, and yet a germane process to tumor survival and progression. JF - Cell cycle (Georgetown, Tex.) AU - To, Kenneth K W AU - Koshiji, Minori AU - Hammer, Stefanie AU - Huang, L Eric AD - Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 881 EP - 882 VL - 4 IS - 7 KW - HIF1A protein, human KW - 0 KW - Hypoxia-Inducible Factor 1, alpha Subunit KW - MSH2 protein, human KW - EC 3.6.1.3 KW - MutS Homolog 2 Protein KW - Index Medicus KW - Humans KW - Colonic Neoplasms -- metabolism KW - MutS Homolog 2 Protein -- metabolism KW - Hypoxia-Inducible Factor 1, alpha Subunit -- metabolism KW - Cell Hypoxia -- genetics KW - Genomic Instability -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68515597?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.atitle=Genetic+instability%3A+the+dark+side+of+the+hypoxic+response.&rft.au=To%2C+Kenneth+K+W%3BKoshiji%2C+Minori%3BHammer%2C+Stefanie%3BHuang%2C+L+Eric&rft.aulast=To&rft.aufirst=Kenneth+K&rft.date=2005-07-01&rft.volume=4&rft.issue=7&rft.spage=881&rft.isbn=&rft.btitle=&rft.title=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.issn=1551-4005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-04 N1 - Date created - 2005-08-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Rabies: an ancient disease that still prevails. AN - 68491640; 16106083 JF - The Indian journal of medical research AU - Madhusudana, S N AD - Department of Neurovirology National Institute of Mental Health & Neuro Sciences Bangalore 560029, India. mshampur@hotmail.com Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 4 EP - 6 VL - 122 IS - 1 SN - 0971-5916, 0971-5916 KW - Index Medicus KW - Rabies virus -- physiology KW - Clinical Laboratory Techniques KW - Humans KW - Bites and Stings -- complications KW - Survival Analysis KW - Prevalence KW - Rabies -- mortality KW - Rabies -- prevention & control KW - Rabies -- epidemiology KW - Rabies -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68491640?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Indian+journal+of+medical+research&rft.atitle=Rabies%3A+an+ancient+disease+that+still+prevails.&rft.au=Madhusudana%2C+S+N&rft.aulast=Madhusudana&rft.aufirst=S&rft.date=2005-07-01&rft.volume=122&rft.issue=1&rft.spage=4&rft.isbn=&rft.btitle=&rft.title=The+Indian+journal+of+medical+research&rft.issn=09715916&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ten-year follow-up of blood lead levels with medical removal protection of shipyard workers. AN - 68477202; 16100939 AB - This cases report compared the short-term changes of BLL with medical removal intervention and follow-up the long-term changes of BLL afterward. During a physical examination in October 1992, a 44-year old shipyard welder was discovered to have a blood lead level (BLL) of 54.1 microg/dl. It was recommended that the shipyard remove this worker from his workplace. In 1993 the BLLs checked for this worker were 36.7 microg/dl in March and 32.0 microg/dl in April. After six months of medical removal, he returned to initial welding work. In 2002, we collected two blood samples from this worker for analysis in May and October. The results were 30.4 microg/dl and 31.6 microg/dl, respectively. Meanwhile, two other welding workers (case 2 and case 3) with BLLs over 40 microg/dl in the survey conducted at the same shipyard in 1992. It took 4 yr to let BLLs downed to less than 40 microg/dl. However, after the blood lead concentration drops to below 40 microg/dl, 10 yr long-term observation indicates that BLLs reduction level off and do not continue to go down in these three cases. JF - Industrial health AU - Yang, Tsan AU - Tung, Ho-Jui AU - Shyr, Jiann-Chian AU - Lai, Ching-Huang AU - Loh, Ching-Hui AU - Liou, Saou-Hsing AD - School of Public Health, National Defense Medical Center, 161 Minchuan East Road, Sec. 6, Nei-Hu, Taipei, Taiwan, ROC. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 611 EP - 614 VL - 43 IS - 3 SN - 0019-8366, 0019-8366 KW - Lead KW - 2P299V784P KW - Index Medicus KW - Ships KW - Humans KW - Adult KW - Welding KW - Follow-Up Studies KW - Male KW - Occupational Exposure -- prevention & control KW - Lead -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68477202?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Industrial+health&rft.atitle=Ten-year+follow-up+of+blood+lead+levels+with+medical+removal+protection+of+shipyard+workers.&rft.au=Yang%2C+Tsan%3BTung%2C+Ho-Jui%3BShyr%2C+Jiann-Chian%3BLai%2C+Ching-Huang%3BLoh%2C+Ching-Hui%3BLiou%2C+Saou-Hsing&rft.aulast=Yang&rft.aufirst=Tsan&rft.date=2005-07-01&rft.volume=43&rft.issue=3&rft.spage=611&rft.isbn=&rft.btitle=&rft.title=Industrial+health&rft.issn=00198366&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-08-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic aspects of pulmonary responses to inhaled pollutants. AN - 68470352; 16092722 AB - Air pollution continues to be a major public health concern in industrialized cities throughout the world. Recent population and epidemiological studies that have associated ozone and particulate exposures with morbidity and mortality outcomes underscore the important detrimental effects of these pollutants on the lung. Inter-individual variation in human responses to air pollutants suggests that some subpopulations are at increased risk to the detrimental effects of pollutant exposure, and it has become clear that genetic background is an important susceptibility factor. Environmental exposures to inhaled pollutants and genetic factors associated with disease risk likely interact in a complex fashion that varies from one population to another. The relationships between the genetic background and disease risk and severity is often evaluated through traditional family-based linkage studies and positional cloning techniques. Case-control studies based on association of disease or disease subphenotypes with candidate genes may have certain advantages over family pedigree studies, and have become useful for understanding complex disease phenotypes. This is based in part on continued development of quantitative analysis and development of mapping technologies. Linkage analyses with genetically standardized animal models are useful to identify genetic determinants of host responses to environmental stimuli. For example, linkage analyses using inbred mice have identified chromosomal segments (quantitative trait loci, QTL) that contain genes that control susceptibility to the lung inflammatory and immune dysfunction responses to ozone, nitrogen dioxide, zinc oxide, and sulfate-associated particles. Candidate genes within the pollutant susceptibility QTLs have been tested for proof-of-concept using gene-targeting and overexpression models. Importantly, significant homology exists between the human and mouse genomes. Therefore, comparative mapping between the human and mouse genomes should yield candidate susceptibility genes that may be tested by association studies in humans. The combined human studies and mouse modeling will provide important insight to understanding genetic factors that contribute to differential susceptibility to pollutants in human populations. JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie AU - Kleeberger, Steven R AD - Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, 111 T.W. Alexander Drive, Bldg 101, Rm. D240, Research Triangle Park, NC 27709, USA. kleeber1@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 147 EP - 153 VL - 57 Suppl 1 SN - 0940-2993, 0940-2993 KW - Air Pollutants KW - 0 KW - Index Medicus KW - Animals KW - Humans KW - Quantitative Trait Loci KW - Disease Models, Animal KW - Mice KW - Genome KW - Lung Diseases -- genetics KW - Lung Diseases -- chemically induced KW - Environmental Illness -- genetics KW - Environmental Illness -- chemically induced KW - Genetic Predisposition to Disease KW - Air Pollutants -- adverse effects KW - Inhalation Exposure -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68470352?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.atitle=Genetic+aspects+of+pulmonary+responses+to+inhaled+pollutants.&rft.au=Kleeberger%2C+Steven+R&rft.aulast=Kleeberger&rft.aufirst=Steven&rft.date=2005-07-01&rft.volume=57+Suppl+1&rft.issue=&rft.spage=147&rft.isbn=&rft.btitle=&rft.title=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.issn=09402993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-08-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - En masse analysis of nascent translation using microarrays. AN - 68432157; 16060370 AB - We report a robust method for studying en masse changes in translation using cDNA arrays. The relative distribution of messenger RNAs (mRNAs) along polysome gradients was monitored by performing cDNA array analysis of each gradient fraction and quantifying the mRNA translational status by regression analysis. Using this strategy to study human carcinoma cells exposed to short-wavelength ultraviolet light (UVC), we identified a subset of 17 translationally induced mRNAs and a subset of 69 translationally repressed mRNAs following UVC irradiation. We describe an effective approach for globally investigating changes in protein biosynthesis. JF - BioTechniques AU - Mazan-Mamczarz, Krystyna AU - Kawai, Tomoko AU - Martindale, Jennifer L AU - Gorospe, Myriam AD - National Institutes of Health, Baltimore, MD, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 61 EP - 2, 64, 66-7 VL - 39 IS - 1 SN - 0736-6205, 0736-6205 KW - Neoplasm Proteins KW - 0 KW - Proteome KW - Index Medicus KW - Gene Expression Regulation, Neoplastic KW - Radiation Dosage KW - Ultraviolet Rays KW - Humans KW - Cell Line, Tumor KW - Proteome -- genetics KW - Protein Modification, Translational -- physiology KW - Colorectal Neoplasms -- metabolism KW - Proteome -- metabolism KW - Oligonucleotide Array Sequence Analysis -- methods KW - Protein Biosynthesis -- physiology KW - Neoplasm Proteins -- metabolism KW - Gene Expression Profiling -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68432157?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BioTechniques&rft.atitle=En+masse+analysis+of+nascent+translation+using+microarrays.&rft.au=Mazan-Mamczarz%2C+Krystyna%3BKawai%2C+Tomoko%3BMartindale%2C+Jennifer+L%3BGorospe%2C+Myriam&rft.aulast=Mazan-Mamczarz&rft.aufirst=Krystyna&rft.date=2005-07-01&rft.volume=39&rft.issue=1&rft.spage=61&rft.isbn=&rft.btitle=&rft.title=BioTechniques&rft.issn=07366205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-23 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3889-94 [12660367] J Mol Diagn. 2003 May;5(2):73-81 [12707371] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8354-9 [12821781] Mol Cell Biol. 2003 Oct;23(20):7083-95 [14517280] Eur J Biochem. 1998 May 1;253(3):531-44 [9654048] Electrophoresis. 1999 Feb;20(2):310-9 [10197438] Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10632-6 [10485877] Mol Cell Biol. 2004 Aug;24(15):6773-87 [15254244] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selected genetic polymorphisms in MGMT, XRCC1, XPD, and XRCC3 and risk of head and neck cancer: a pooled analysis. AN - 68056288; 16030112 AB - Tobacco and alcohol consumption are the major risk factors for head and neck cancer, likely due to DNA-damaging processes. Genetic variations in DNA repair genes may affect an individual's susceptibility to head and neck cancer. Pooling data and DNA specimens from three case-control studies in western Washington State, North Carolina, and Puerto Rico, totaling 555 cases (430 whites) and 792 controls (695 whites), we studied the risk of head and neck cancer in relation to common nonsynonymous single-nucleotide polymorphisms in four DNA repair genes: MGMT (Leu84Phe and Ile143Val), XRCC1 (Arg399Gln), XPD (Lys751Gln), and XRCC3 (Thr241Met). All single-nucleotide polymorphisms were assayed in a single laboratory. Among whites, carriage of the MGMT Phe84 [odds ratio (OR), 0.71; 95% confidence interval (95% CI), 0.51-0.98] or Val143 (OR, 0.66; 95% CI, 0.47-0.92) allele was associated with a decreased risk of head and neck cancer; the haplotype distribution for MGMT differed significantly between cases and controls (covariate-adjusted global permutation test, P = 0.012). The XRCC1 GlnGln399 genotype was also associated with decreased risk among whites (OR, 0.56; 95% CI, 0.32-0.94), whereas XPD751 and XRCC3241 were not associated with risk. Alcohol-related risks tended to vary with DNA repair genotypes, especially for MGMT variants, whereas no effect modification was noted with tobacco use. Consistent findings from three case-control studies suggest that selected DNA repair enzymes may play a role in head and neck carcinogenesis. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Huang, Wen-Yi AU - Olshan, Andrew F AU - Schwartz, Stephen M AU - Berndt, Sonja I AU - Chen, Chu AU - Llaca, Victor AU - Chanock, Stephen J AU - Fraumeni, Joseph F AU - Hayes, Richard B AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, EPS 8113, MSC 7240, Bethesda, MD 20892, USA. huangw@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1747 EP - 1753 VL - 14 IS - 7 SN - 1055-9965, 1055-9965 KW - Index Medicus KW - Genotype KW - Risk Factors KW - Humans KW - Case-Control Studies KW - Alcohol Drinking -- adverse effects KW - Smoking -- adverse effects KW - Middle Aged KW - Male KW - Female KW - DNA Repair -- genetics KW - Polymorphism, Single Nucleotide KW - Head and Neck Neoplasms -- etiology KW - Head and Neck Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056288?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Selected+genetic+polymorphisms+in+MGMT%2C+XRCC1%2C+XPD%2C+and+XRCC3+and+risk+of+head+and+neck+cancer%3A+a+pooled+analysis.&rft.au=Huang%2C+Wen-Yi%3BOlshan%2C+Andrew+F%3BSchwartz%2C+Stephen+M%3BBerndt%2C+Sonja+I%3BChen%2C+Chu%3BLlaca%2C+Victor%3BChanock%2C+Stephen+J%3BFraumeni%2C+Joseph+F%3BHayes%2C+Richard+B&rft.aulast=Huang&rft.aufirst=Wen-Yi&rft.date=2005-07-01&rft.volume=14&rft.issue=7&rft.spage=1747&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-12 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Endometrial carcinoma risks among menopausal estrogen plus progestin and unopposed estrogen users in a cohort of postmenopausal women. AN - 68055763; 16030108 AB - Because unopposed estrogen substantially increases endometrial carcinoma risk, estrogen plus progestin is one menopausal hormone therapy formulation for women who have not had a hysterectomy. However, endometrial carcinoma risks among estrogen plus progestin users and among former unopposed estrogen users are not firmly established. We evaluated endometrial carcinoma risks associated with estrogen plus progestin and unopposed estrogen therapies in 30,379 postmenopausal Breast Cancer Detection Demonstration Project follow-up study participants. We ascertained hormone therapy use and other risk factors during telephone interviews and mailed questionnaires between 1979 and 1998. We identified 541 endometrial carcinomas via self-report, medical records, the National Death Index, and state cancer registries. Poisson regression generated time-dependent rate ratios (RR) and 95% confidence intervals (95% CI). Endometrial carcinoma was significantly associated with estrogen plus progestin only use (n = 68 cancers; RR, 2.6; 95% CI, 1.9-3.5), including both sequential (progestin or =10 years after last use (RR, 1.5; 95% CI, 1.0-2.1). Both estrogen plus progestin regimens significantly increased endometrial carcinoma risk in this study. Risks among unopposed estrogen users remained elevated long after last use. The prospect that all estrogen plus progestin regimens increase endometrial carcinoma risk deserves continued research. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Lacey, James V AU - Brinton, Louise A AU - Lubin, Jay H AU - Sherman, Mark E AU - Schatzkin, Arthur AU - Schairer, Catherine AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Department of Health and Human Services, Bethesda, Maryland, USA. jimlacey@nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1724 EP - 1731 VL - 14 IS - 7 SN - 1055-9965, 1055-9965 KW - Estrogens KW - 0 KW - Progestins KW - Index Medicus KW - Registries KW - Aged, 80 and over KW - Humans KW - Cohort Studies KW - Surveys and Questionnaires KW - Aged KW - Middle Aged KW - United States -- epidemiology KW - Female KW - Endometrial Neoplasms -- chemically induced KW - Postmenopause KW - Estrogen Replacement Therapy -- adverse effects KW - Progestins -- adverse effects KW - Endometrial Neoplasms -- epidemiology KW - Progestins -- administration & dosage KW - Estrogens -- adverse effects KW - Estrogens -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68055763?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Endometrial+carcinoma+risks+among+menopausal+estrogen+plus+progestin+and+unopposed+estrogen+users+in+a+cohort+of+postmenopausal+women.&rft.au=Lacey%2C+James+V%3BBrinton%2C+Louise+A%3BLubin%2C+Jay+H%3BSherman%2C+Mark+E%3BSchatzkin%2C+Arthur%3BSchairer%2C+Catherine&rft.aulast=Lacey&rft.aufirst=James&rft.date=2005-07-01&rft.volume=14&rft.issue=7&rft.spage=1724&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-12 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - GSTM1, GSTT1, and GSTP1 polymorphisms and risk of advanced colorectal adenoma. AN - 68055606; 16030123 AB - Cigarette smoking is a risk factor for colon adenoma. The glutathione S-transferase enzymes are involved in the detoxification of carcinogenic compounds including those found in tobacco smoke, and thus, may be important modifiers of individual risk of developing this disease. We examined the prevalence of GSTM1 and GSTT1 gene deletions, and two GSTP1 polymorphisms in 772 cases with advanced colorectal adenomas (>1 cm, villous elements or high-grade dysplasia) of the distal colon (descending or sigmoid colon or rectum) and 777 sigmoidoscopy negative controls enrolled in the screening arm of the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Epidemiologic data on smoking was collected by self-administered questionnaire and DNA was extracted from whole blood or buffy coat. For GSTM1 and GSTT1, we used a newly developed TaqMan-based assay capable of discriminating heterozygous (+/-) individuals from those with two active alleles (+/+) and homozygous deletions (-/-). For GSTP1, the I105V and the A114V substitutions were identified using end point 5' nuclease assays (TaqMan). Adjusted odds ratios (OR) and 95% confidence intervals (95% CI) were determined using unconditional logistic regression, controlling for age, race, and gender. Advanced adenoma risk was increased in current/former smokers (OR, 1.4; 95% CI, 1.2-1.8). Risks were decreased in subjects with > or =1 inactive GSTM1 alleles (OR, 0.6; 95% CI, 0.4-0.9); and the association was independent of smoking status (P interaction = 0.59). Having > or =1 inactive GSTT1 allele was associated with increased risk among smokers (OR, 1.4; 95% CI, 1.1-1.9; P trend = 0.02) but not among never smokers (OR, 0.9; 95% CI, 0.6-1.3) and a significant interaction between smoking and genotype was observed (P interaction = 0.05). In summary, this is the first study to report associations between colorectal adenomas and GSTM1 wild-type and GSTT1 null allele among smokers. These findings only became apparent using a newly developed assay able to distinguish heterozygous from wild-type individuals. Our data provide evidence that phenotypic differences between these two groups exist. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Moore, Lee E AU - Huang, Wen-Yi AU - Chatterjee, Nilanjan AU - Gunter, Marc AU - Chanock, Stephen AU - Yeager, Meredith AU - Welch, Bob AU - Pinsky, Paul AU - Weissfeld, Joel AU - Hayes, Richard B AD - National Cancer Institute, 6120 Executive Boulevard, EPS 7034, Bethesda, MD 20892-7240, USA. moorele@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1823 EP - 1827 VL - 14 IS - 7 SN - 1055-9965, 1055-9965 KW - Isoenzymes KW - 0 KW - glutathione S-transferase T1 KW - EC 2.5.1.- KW - GSTP1 protein, human KW - EC 2.5.1.18 KW - Glutathione S-Transferase pi KW - Glutathione Transferase KW - glutathione S-transferase M1 KW - Index Medicus KW - Genotype KW - Risk KW - Humans KW - Smoking -- adverse effects KW - Aged KW - Middle Aged KW - Male KW - Female KW - Polymorphism, Genetic KW - Colorectal Neoplasms -- etiology KW - Adenoma -- etiology KW - Glutathione Transferase -- genetics KW - Colorectal Neoplasms -- genetics KW - Adenoma -- genetics KW - Isoenzymes -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68055606?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=GSTM1%2C+GSTT1%2C+and+GSTP1+polymorphisms+and+risk+of+advanced+colorectal+adenoma.&rft.au=Moore%2C+Lee+E%3BHuang%2C+Wen-Yi%3BChatterjee%2C+Nilanjan%3BGunter%2C+Marc%3BChanock%2C+Stephen%3BYeager%2C+Meredith%3BWelch%2C+Bob%3BPinsky%2C+Paul%3BWeissfeld%2C+Joel%3BHayes%2C+Richard+B&rft.aulast=Moore&rft.aufirst=Lee&rft.date=2005-07-01&rft.volume=14&rft.issue=7&rft.spage=1823&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-12 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic polymorphisms of eosinophil-derived neurotoxin and eosinophil cationic protein in tropical pulmonary eosinophilia. AN - 68028347; 16014847 AB - Because eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are critical in the pathogenesis of tropical pulmonary eosinophilia (TPE), we analyzed genetic polymorphisms of both in 181 individuals from southern India with varying clinical manifestations of Wuchereria bancrofti infection (including 26 with TPE). Using haplotype frequency analysis, we identified four known (of nine) and two novel haplotypes for EDN (1, 2, 7, 8, 10, and 11). For ECP, five (of seven known) haplotypes (1-5) were identified. Although we found no significant association between frequencies of EDN and ECP polymorphisms and TPE development, we observed a unique pattern of EDN and ECP polymorphism distribution among this population. Genotype TT at locus 1088 of ECP in one TPE patient was not observed in any other clinical group. Although the EDN and ECP polymorphisms appear unlikely to be associated with the development of TPE, further analyses will be more definitive. JF - The American journal of tropical medicine and hygiene AU - Kim, Yae-Jean AU - Kumaraswami, V AU - Choi, Eunhwa AU - Mu, Jianbing AU - Follmann, Dean A AU - Zimmerman, Peter AU - Nutman, Thomas B AD - Laboratory of Malaria and Vector Research, Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. yjkim@niaid.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 125 EP - 130 VL - 73 IS - 1 SN - 0002-9637, 0002-9637 KW - Eosinophil-Derived Neurotoxin KW - EC 3.1.- KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Genotype KW - Animals KW - Wuchereria bancrofti KW - Gene Frequency KW - Filariasis -- complications KW - Filariasis -- genetics KW - Humans KW - National Institutes of Health (U.S.) KW - India KW - Eosinophils -- physiology KW - Polymorphism, Genetic KW - Pulmonary Eosinophilia -- complications KW - Pulmonary Eosinophilia -- genetics KW - Eosinophil-Derived Neurotoxin -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68028347?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+tropical+medicine+and+hygiene&rft.atitle=Genetic+polymorphisms+of+eosinophil-derived+neurotoxin+and+eosinophil+cationic+protein+in+tropical+pulmonary+eosinophilia.&rft.au=Kim%2C+Yae-Jean%3BKumaraswami%2C+V%3BChoi%2C+Eunhwa%3BMu%2C+Jianbing%3BFollmann%2C+Dean+A%3BZimmerman%2C+Peter%3BNutman%2C+Thomas+B&rft.aulast=Kim&rft.aufirst=Yae-Jean&rft.date=2005-07-01&rft.volume=73&rft.issue=1&rft.spage=125&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+tropical+medicine+and+hygiene&rft.issn=00029637&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-07-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CONF T1 - Developmental origins and environmental influences--Introduction. NIEHS symposium. AN - 68023470; 15959884 JF - Birth defects research. Part A, Clinical and molecular teratology AU - Heindel, Jerrold J AU - Levin, Edward Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 469 VL - 73 IS - 7 KW - Index Medicus KW - Models, Animal KW - Risk Factors KW - Humans KW - Adipocytes -- metabolism KW - Genetic Predisposition to Disease KW - Obesity -- etiology KW - Obesity -- genetics KW - Environmental Exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68023470?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.atitle=Developmental+origins+and+environmental+influences--Introduction.+NIEHS+symposium.&rft.au=Heindel%2C+Jerrold+J%3BLevin%2C+Edward&rft.aulast=Heindel&rft.aufirst=Jerrold&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=469&rft.isbn=&rft.btitle=&rft.title=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.issn=15420752&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-07-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Developmental exposure to estrogenic compounds and obesity. AN - 68022695; 15959888 JF - Birth defects research. Part A, Clinical and molecular teratology AU - Newbold, Retha R AU - Padilla-Banks, Elizabeth AU - Snyder, Ryan J AU - Jefferson, Wendy N AD - Developmental Endocrinology Section, Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. newbold1@nichs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 478 EP - 480 VL - 73 IS - 7 SN - 1542-0752, 1542-0752 KW - Estrogens KW - 0 KW - Diethylstilbestrol KW - 731DCA35BT KW - Genistein KW - DH2M523P0H KW - Index Medicus KW - Models, Animal KW - Animals KW - Genistein -- pharmacology KW - Body Weight -- drug effects KW - Mice KW - Fetal Weight -- drug effects KW - Female KW - Prenatal Exposure Delayed Effects KW - Pregnancy KW - Obesity -- etiology KW - Estrogens -- pharmacology KW - Pregnancy, Animal KW - Diethylstilbestrol -- pharmacology KW - Estrogens -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68022695?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.atitle=Developmental+exposure+to+estrogenic+compounds+and+obesity.&rft.au=Newbold%2C+Retha+R%3BPadilla-Banks%2C+Elizabeth%3BSnyder%2C+Ryan+J%3BJefferson%2C+Wendy+N&rft.aulast=Newbold&rft.aufirst=Retha&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=478&rft.isbn=&rft.btitle=&rft.title=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.issn=15420752&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-27 N1 - Date created - 2005-07-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Suppression of immune-mediated ocular inflammation in mice by interleukin 1 receptor antagonist administration. AN - 68019764; 16009838 AB - To evaluate the effects of an interleukin 1 receptor antagonist (IL-1RA) on the development of immune-mediated ocular inflammation in mice. Recombinant, human, nonglycosylated IL-1RA (anakinra [kineret]) was tested for its inhibitory effects in 2 systems: (1) experimental autoimmune uveitis induced by interphotoreceptor retinoid-binding protein in B10.A mice using routine procedures and evaluated by clinical and histological examination, and (2) ocular inflammation in mice induced by transfer of hen egg lysozyme-specific T cells to hen egg lysozyme-transgenic mice. Treatment with IL-1RA included daily subcutaneous injections of the drug, at 300 and 500 mg/kg, or phosphate-buffered saline as control. Mean +/- SE experimental autoimmune uveitis scores of histological ocular changes of the mice at day 14 postimmunization with interphotoreceptor retinoid-binding protein were 1.5 +/- 0.3 in control mice; 1.0 +/- 0.4 in 300-mg/kg anakinra-treated mice; and 0.5 +/- 0.2 in 500- mg/kg anakinra-treated mice (P = .004). There was a corresponding decrease in the cellular immune response and cytokine production of immune cells in treated mice. Suppression of ocular inflammation by anakinra in the transfer system was also observed (P = .04). Human IL-1RA suppresses immune-mediated ocular inflammation in mice, affecting both the afferent and efferent components of the pathogenic immune response.Clinical Relevance Systemic administration of IL-1RA may have clinical application in the management of patients with uveitis. JF - Archives of ophthalmology (Chicago, Ill. : 1960) AU - Lim, Wee-Kiak AU - Fujimoto, Chiaki AU - Ursea, Roxana AU - Mahesh, Sankaranarayana Pillai AU - Silver, Phyllis AU - Chan, Chi-Chao AU - Gery, Igal AU - Nussenblatt, Robert B AD - Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 957 EP - 963 VL - 123 IS - 7 SN - 0003-9950, 0003-9950 KW - Cytokines KW - 0 KW - Eye Proteins KW - IL1RN protein, human KW - Il1rn protein, mouse KW - Interleukin 1 Receptor Antagonist Protein KW - Recombinant Proteins KW - Retinol-Binding Proteins KW - Sialoglycoproteins KW - interstitial retinol-binding protein KW - hen egg lysozyme KW - EC 3.2.1.- KW - Muramidase KW - EC 3.2.1.17 KW - Abridged Index Medicus KW - Index Medicus KW - Th1 Cells -- immunology KW - Animals KW - Eye Proteins -- toxicity KW - Retinol-Binding Proteins -- toxicity KW - Cytokines -- biosynthesis KW - Muramidase -- immunology KW - Disease Models, Animal KW - Mice KW - Mice, Transgenic KW - Adoptive Transfer KW - Retinol-Binding Proteins -- immunology KW - Eye Proteins -- immunology KW - Lymphocyte Activation -- immunology KW - Immunity, Cellular -- drug effects KW - Immunotherapy, Adoptive KW - Injections, Subcutaneous KW - Female KW - Immunosuppression KW - Uveitis -- prevention & control KW - Autoimmune Diseases -- prevention & control KW - Uveitis -- pathology KW - Autoimmune Diseases -- pathology KW - Sialoglycoproteins -- administration & dosage KW - Uveitis -- immunology KW - Recombinant Proteins -- administration & dosage KW - Autoimmune Diseases -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68019764?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+ophthalmology+%28Chicago%2C+Ill.+%3A+1960%29&rft.atitle=Suppression+of+immune-mediated+ocular+inflammation+in+mice+by+interleukin+1+receptor+antagonist+administration.&rft.au=Lim%2C+Wee-Kiak%3BFujimoto%2C+Chiaki%3BUrsea%2C+Roxana%3BMahesh%2C+Sankaranarayana+Pillai%3BSilver%2C+Phyllis%3BChan%2C+Chi-Chao%3BGery%2C+Igal%3BNussenblatt%2C+Robert+B&rft.aulast=Lim&rft.aufirst=Wee-Kiak&rft.date=2005-07-01&rft.volume=123&rft.issue=7&rft.spage=957&rft.isbn=&rft.btitle=&rft.title=Archives+of+ophthalmology+%28Chicago%2C+Ill.+%3A+1960%29&rft.issn=00039950&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-07-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neurologic symptoms in licensed private pesticide applicators in the agricultural health study. AN - 68012000; 16002376 AB - Exposure to high levels of many pesticides has both acute and long-term neurologic consequences, but little is known about the neurotoxicity of chronic exposure to moderate levels of pesticides. We analyzed cross-sectional data from 18,782 white male licensed private pesticide applicators enrolled in the Agricultural Health Study in 1993-1997. Applicators provided information on lifetime pesticide use and 23 neurologic symptoms typically associated with pesticide intoxication. An indicator of more symptoms (> or = 10 vs. 500 days, compared with never users. A modest association for fumigants [> 50 days, 1.50 (1.24-1.81)] and weaker relationships for herbicides [> 500 days, 1.32 (0.99-1.75)] and fungicides [> 50 days, 1.23 (1.00-1.50)] were observed. Pesticide use within the year before enrollment was not associated with symptom count. Only associations with insecticides and fumigants persisted when all four pesticide groups were examined simultaneously. Among chemical classes of insecticides, associations were strongest for organophosphates and organochlorines. Associations with cumulative exposure persisted after excluding individuals who had a history of pesticide poisoning or had experienced an event involving high personal pesticide exposure. These results suggest that self-reported neurologic symptoms are associated with cumulative exposure to moderate levels of fumigants and organophosphate and organochlorine insecticides, regardless of recent exposure or history of poisoning. JF - Environmental health perspectives AU - Kamel, Freya AU - Engel, Lawrence S AU - Gladen, Beth C AU - Hoppin, Jane A AU - Alavanja, Michael C R AU - Sandler, Dale P AD - National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. kamel@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 877 EP - 882 VL - 113 IS - 7 SN - 0091-6765, 0091-6765 KW - Agrochemicals KW - 0 KW - Hydrocarbons, Chlorinated KW - Organophosphorus Compounds KW - Pesticides KW - Index Medicus KW - Cross-Sectional Studies KW - Humans KW - Adult KW - Aged KW - Organophosphorus Compounds -- adverse effects KW - Middle Aged KW - North Carolina -- epidemiology KW - Hydrocarbons, Chlorinated -- adverse effects KW - Adolescent KW - Male KW - Iowa -- epidemiology KW - Agrochemicals -- adverse effects KW - Occupational Exposure KW - Nervous System Diseases -- epidemiology KW - Agricultural Workers' Diseases -- epidemiology KW - Agricultural Workers' Diseases -- chemically induced KW - Nervous System Diseases -- chemically induced KW - Pesticides -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68012000?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Neurologic+symptoms+in+licensed+private+pesticide+applicators+in+the+agricultural+health+study.&rft.au=Kamel%2C+Freya%3BEngel%2C+Lawrence+S%3BGladen%2C+Beth+C%3BHoppin%2C+Jane+A%3BAlavanja%2C+Michael+C+R%3BSandler%2C+Dale+P&rft.aulast=Kamel&rft.aufirst=Freya&rft.date=2005-07-01&rft.volume=113&rft.issue=7&rft.spage=877&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-07-08 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Neurotoxicology. 1999 Oct;20(5):819-26 [10591517] Am J Ind Med. 2004 Dec;46(6):599-606 [15551369] Environ Health Perspect. 2000 Apr;108(4):293-300 [10753086] Occup Environ Med. 2000 Mar;57(3):195-200 [10810102] Int Arch Occup Environ Health. 2000 Aug;73(6):362-8 [11007338] Lancet. 2001 Mar 31;357(9261):1014-6 [11293598] Occup Environ Med. 2001 Nov;58(11):702-10 [11600725] Epidemiology. 2002 Jan;13(1):94-9 [11805592] Int J Occup Environ Health. 2002 Jan-Mar;8(1):27-34 [11843437] J Expo Anal Environ Epidemiol. 2002 Sep;12(5):313-8 [12198579] J Occup Environ Med. 2003 Feb;45(2):118-22 [12625227] Am J Ind Med. 2003 Sep;44(3):254-64 [12929145] Environ Health Perspect. 2004 Jun;112(9):950-8 [15198914] Res Commun Chem Pathol Pharmacol. 1974 Oct;9(2):325-37 [4438838] Arch Gen Psychiatry. 1976 Feb;33(2):225-8 [1252099] Neurotoxicology. 1986 Fall;7(3):137-56 [3822255] Arch Environ Health. 1988 Jan-Feb;43(1):38-45 [3355242] Am J Public Health. 1994 Mar;84(3):446-51 [8129063] Occup Environ Med. 1995 Oct;52(10):648-53 [7489054] Arch Environ Health. 1995 Nov-Dec;50(6):440-4 [8572722] Environ Health Perspect. 1996 Apr;104(4):362-9 [8732939] Am J Ind Med. 1997 Feb;31(2):233-42 [9028440] Lancet. 1997 Apr 26;349(9060):1239-43 [9130958] Occup Environ Med. 1997 May;54(5):343-50 [9196457] Occup Med. 1997 Apr-Jun;12(2):291-304 [9220487] Am J Ind Med. 1997 Nov;32(5):487-96 [9327072] Scand J Work Environ Health. 1998 Feb;24(1):18-29 [9562397] Scand J Work Environ Health. 1998 Jun;24(3):213-9 [9710374] Am J Public Health. 1998 Dec;88(12):1774-80 [9842373] Environ Res. 1999 Feb;80(2 Pt 1):172-9 [10092410] Environ Res. 1999 Feb;80(2 Pt 1):180-6 [10092411] Occup Environ Med. 1999 Jul;56(7):449-53 [10472315] Comment In: Environ Health Perspect. 2005 Dec;113(12):A800; author reply A800-1 [16330330] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Personalized exposure assessment: promising approaches for human environmental health research. AN - 68010780; 16002370 AB - New technologies and methods for assessing human exposure to chemicals, dietary and lifestyle factors, infectious agents, and other stressors provide an opportunity to extend the range of human health investigations and advance our understanding of the relationship between environmental exposure and disease. An ad hoc Committee on Environmental Exposure Technology Development was convened to identify new technologies and methods for deriving personalized exposure measurements for application to environmental health studies. The committee identified a "toolbox" of methods for measuring external (environmental) and internal (biologic) exposure and assessing human behaviors that influence the likelihood of exposure to environmental agents. The methods use environmental sensors, geographic information systems, biologic sensors, toxicogenomics, and body burden (biologic) measurements. We discuss each of the methods in relation to current use in human health research; specific gaps in the development, validation, and application of the methods are highlighted. We also present a conceptual framework for moving these technologies into use and acceptance by the scientific community. The framework focuses on understanding complex human diseases using an integrated approach to exposure assessment to define particular exposure-disease relationships and the interaction of genetic and environmental factors in disease occurrence. Improved methods for exposure assessment will result in better means of monitoring and targeting intervention and prevention programs. JF - Environmental health perspectives AU - Weis, Brenda K AU - Balshaw, David AU - Barr, John R AU - Brown, David AU - Ellisman, Mark AU - Lioy, Paul AU - Omenn, Gilbert AU - Potter, John D AU - Smith, Martyn T AU - Sohn, Lydia AU - Suk, William A AU - Sumner, Susan AU - Swenberg, James AU - Walt, David R AU - Watkins, Simon AU - Thompson, Claudia AU - Wilson, Samuel H AD - Division of Extramural Research and Training, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA. weis@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 840 EP - 848 VL - 113 IS - 7 SN - 0091-6765, 0091-6765 KW - Biomarkers KW - 0 KW - Environmental Pollutants KW - Xenobiotics KW - Index Medicus KW - Humans KW - Body Burden KW - Toxicogenetics KW - Research KW - Geographic Information Systems KW - Environmental Exposure KW - Environmental Monitoring -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68010780?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Personalized+exposure+assessment%3A+promising+approaches+for+human+environmental+health+research.&rft.au=Weis%2C+Brenda+K%3BBalshaw%2C+David%3BBarr%2C+John+R%3BBrown%2C+David%3BEllisman%2C+Mark%3BLioy%2C+Paul%3BOmenn%2C+Gilbert%3BPotter%2C+John+D%3BSmith%2C+Martyn+T%3BSohn%2C+Lydia%3BSuk%2C+William+A%3BSumner%2C+Susan%3BSwenberg%2C+James%3BWalt%2C+David+R%3BWatkins%2C+Simon%3BThompson%2C+Claudia%3BWilson%2C+Samuel+H&rft.aulast=Weis&rft.aufirst=Brenda&rft.date=2005-07-01&rft.volume=113&rft.issue=7&rft.spage=840&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-07-08 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nat Genet. 2002 Dec;32 Suppl:533-40 [12454650] Nat Med. 2002 Dec;8(12):1439-44 [12447357] Environ Health Perspect. 2003 Jan;111(1):115-22 [12515689] Genet Med. 2002 Nov-Dec;4(6 Suppl):21S-26S [12544483] J Cell Biochem Suppl. 2002;39:154-61 [12552615] Cancer Epidemiol Biomarkers Prev. 2003 Feb;12(2):157-60 [12582026] Adv Drug Deliv Rev. 2003 Feb 24;55(3):393-401 [12628323] Chem Res Toxicol. 2003 Mar;16(3):295-303 [12641429] Toxicol Appl Pharmacol. 2003 Mar 15;187(3):137-46 [12662897] Acta Biochim Pol. 2003;50(1):61-8 [12673347] J Nutr. 2003 Jun;133(6 Suppl 1):2078S-2083S [12771369] J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 25;794(1):137-48 [12888206] Proteomics. 2004 Aug;4(8):2363-5 [15274131] Analyst. 2004 Aug;129(8):745-50 [15284919] Urol Oncol. 2004 Jul-Aug;22(4):322-8 [15283891] N Engl J Med. 2004 Aug 5;351(6):533-42 [15295046] Chest. 2004 Aug;126(2 Suppl):105S-110S; 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AN - 68010637; 16000290 AB - Mesothelin is a glycoprotein that is overexpressed in several human tumors, including mesotheliomas and ovarian cancers, and has been identified as a potential target for therapy. We evaluated the biodistribution and tumor-targeting ability of an antimesothelin tetravalent single-chain Fv-streptavidin fusion protein (SS1scFvSA) in mice. SS1scFvSA was labeled with 125I or 111In for evaluation of internalization in vitro and for optimization of its biodistribution. The A431-K5 mesothelin transfected cell line was used as the target. We used a 3-step pretargeting approach consisting of injections of (i) SS1scFvSA, followed 20 h later by (ii) a synthetic clearing agent, and (iii) 4 h later, radiolabeled (111In, 88Y/90Y, or 177Lu) 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)-biotin. To optimize the tumor uptake, the effect of the specific activity of 111In-DOTA-biotin was evaluated. Approximately 60% of SS1sc FvSA internalized within 6 h. The optimal dose of SS1scFvSA for pretargeting was 600 microg. Decreasing the specific activity of DOTA-biotin by administering 0.1-5 microg of DOTA-biotin resulted in tumor uptake decreasing from 31.8 to 5.5 %ID/g (percentage injected dose per gram) at 2 h. Pretargeted therapy of A431-K5 tumor with 90Y doses of 11.1-32.4 MBq resulted in a dose-dependent tumor response. With 32.4 MBq, 86% of mice survived tumor free for 110 d. All nontreated mice died, with a median survival of 16 d. SS1scFvSA localized in the mesothelin-expressing tumor, resulting in a high accumulation of radiolabeled DOTA-biotin. The specific activity of DOTA-biotin had a significant effect on its tumor uptake. Therapeutic tumor doses were obtained without dose-limiting toxicity. JF - Journal of nuclear medicine : official publication, Society of Nuclear Medicine AU - Sato, Noriko AU - Hassan, Raffit AU - Axworthy, Donald B AU - Wong, Karen J AU - Yu, Sarah AU - Theodore, Louis J AU - Lin, Yukang AU - Park, Luke AU - Brechbiel, Martin W AU - Pastan, Ira AU - Paik, Chang H AU - Carrasquillo, Jorge A AD - Nuclear Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1201 EP - 1209 VL - 46 IS - 7 SN - 0161-5505, 0161-5505 KW - GPI-Linked Proteins KW - 0 KW - Immunoglobulin Fragments KW - Iodine Radioisotopes KW - Membrane Glycoproteins KW - Radiopharmaceuticals KW - Recombinant Fusion Proteins KW - mesothelin KW - Streptavidin KW - 9013-20-1 KW - Index Medicus KW - Animals KW - Recombinant Fusion Proteins -- pharmacokinetics KW - Humans KW - Metabolic Clearance Rate KW - Cell Line, Tumor KW - Mice KW - Mice, Nude KW - Organ Specificity KW - Tissue Distribution KW - Immunoglobulin Fragments -- therapeutic use KW - Treatment Outcome KW - Immunoglobulin Fragments -- metabolism KW - Drug Delivery Systems -- methods KW - Recombinant Fusion Proteins -- therapeutic use KW - Female KW - Survival Analysis KW - Streptavidin -- pharmacokinetics KW - Radiopharmaceuticals -- therapeutic use KW - Iodine Radioisotopes -- therapeutic use KW - Radiopharmaceuticals -- pharmacokinetics KW - Carcinoma, Squamous Cell -- metabolism KW - Streptavidin -- therapeutic use KW - Carcinoma, Squamous Cell -- radiotherapy KW - Radioimmunotherapy -- methods KW - Iodine Radioisotopes -- pharmacokinetics KW - Membrane Glycoproteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68010637?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+nuclear+medicine+%3A+official+publication%2C+Society+of+Nuclear+Medicine&rft.atitle=Pretargeted+radioimmunotherapy+of+mesothelin-expressing+cancer+using+a+tetravalent+single-chain+Fv-streptavidin+fusion+protein.&rft.au=Sato%2C+Noriko%3BHassan%2C+Raffit%3BAxworthy%2C+Donald+B%3BWong%2C+Karen+J%3BYu%2C+Sarah%3BTheodore%2C+Louis+J%3BLin%2C+Yukang%3BPark%2C+Luke%3BBrechbiel%2C+Martin+W%3BPastan%2C+Ira%3BPaik%2C+Chang+H%3BCarrasquillo%2C+Jorge+A&rft.aulast=Sato&rft.aufirst=Noriko&rft.date=2005-07-01&rft.volume=46&rft.issue=7&rft.spage=1201&rft.isbn=&rft.btitle=&rft.title=Journal+of+nuclear+medicine+%3A+official+publication%2C+Society+of+Nuclear+Medicine&rft.issn=01615505&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-23 N1 - Date created - 2005-07-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The NIEHS and the National Toxicology Program: an integrated scientific vision. AN - 68010410; 16002356 JF - Environmental health perspectives AU - Portier, Christopher J AU - Schwartz, David A AD - portier@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1 VL - 113 IS - 7 SN - 0091-6765, 0091-6765 KW - Index Medicus KW - United States KW - National Institutes of Health (U.S.) KW - Toxicity Tests KW - Toxicogenetics KW - Research KW - Environmental Health KW - Government Programs KW - Toxicology -- education UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68010410?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=The+NIEHS+and+the+National+Toxicology+Program%3A+an+integrated+scientific+vision.&rft.au=Portier%2C+Christopher+J%3BSchwartz%2C+David+A&rft.aulast=Portier&rft.aufirst=Christopher&rft.date=2005-07-01&rft.volume=113&rft.issue=7&rft.spage=A440&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-07-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Association of Paclitaxel pharmacokinetics with the development of peripheral neuropathy in patients with advanced cancer. AN - 68007138; 16000582 AB - The shortening of infusion time from 3 to 1 hour decreases the systemic exposure (area under the curve, AUC) of total and unbound paclitaxel but increases the AUC of its vehicle Cremophor EL, whereas the time above total paclitaxel concentrations of 0.05 micromol/L (T >0.05) remains almost constant. As both Cremophor EL and paclitaxel are neurotoxic, we evaluated their pharmacodynamic effects on the development of peripheral neuropathy as the most important nonhematologic toxicity. Patients with advanced cancer of different origin were randomized to receive a maximum of 12 weekly-given 1- or 3-hour infusions of 100 mg/m2 paclitaxel (Taxol). Twenty-four patients were assessable for both pharmacokinetics and peripheral neuropathy development evaluated by a clinical scoring system including sensory symptoms, strength, tendon reflexes, and vibratory sense. Patients with peripheral neuropathy development (n=14) received more weeks of therapy (P=0.056) and showed significantly higher T(>0.05) (P=0.022) and overall systemic drug exposures (weeks of therapy x AUC) for total paclitaxel (P=0.002) and unbound paclitaxel (P=0.003) than those without peripheral neuropathy. In Kaplan-Meier analyses, T(>0.05) > or = 10.6 hours (P=0.023), AUC of total paclitaxel > or = 4.7 microg/mL x hour (P = 0.047), and AUC of unbound paclitaxel > or = 0.375 microg/mL x hour (P = 0.095) were identified as being potential factors for peripheral neuropathy development. In a Cox regression analysis, only T(>0.05) > or = 10.6 hours remained as an independent risk factor (relative risk, 18.43; P = 0.036) after adjusting for prior vincamycin (relative risk, 11.28; P = 0.038). From the results obtained in this study, it is concluded that exposure to paclitaxel but not Cremophor EL is associated with peripheral neuropathy development. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Mielke, Stephan AU - Sparreboom, Alex AU - Steinberg, Seth M AU - Gelderblom, Hans AU - Unger, Clemens AU - Behringer, Dirk AU - Mross, Klaus AD - Department of Hematology and Oncology, University of Freiburg Medical Center, Freiburg, Germany. mielkes@nhlbi.nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 4843 EP - 4850 VL - 11 IS - 13 SN - 1078-0432, 1078-0432 KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Paclitaxel KW - P88XT4IS4D KW - Index Medicus KW - Infusion Pumps KW - Area Under Curve KW - Humans KW - Adult KW - Treatment Outcome KW - Aged KW - Middle Aged KW - Time Factors KW - Male KW - Female KW - Proportional Hazards Models KW - Paclitaxel -- administration & dosage KW - Antineoplastic Agents, Phytogenic -- pharmacokinetics KW - Neoplasms -- drug therapy KW - Paclitaxel -- adverse effects KW - Antineoplastic Agents, Phytogenic -- adverse effects KW - Paclitaxel -- pharmacokinetics KW - Peripheral Nervous System Diseases -- chemically induced KW - Antineoplastic Agents, Phytogenic -- administration & dosage KW - Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68007138?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Association+of+Paclitaxel+pharmacokinetics+with+the+development+of+peripheral+neuropathy+in+patients+with+advanced+cancer.&rft.au=Mielke%2C+Stephan%3BSparreboom%2C+Alex%3BSteinberg%2C+Seth+M%3BGelderblom%2C+Hans%3BUnger%2C+Clemens%3BBehringer%2C+Dirk%3BMross%2C+Klaus&rft.aulast=Mielke&rft.aufirst=Stephan&rft.date=2005-07-01&rft.volume=11&rft.issue=13&rft.spage=4843&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-07-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phenotypic effects of calorie restriction and insulin-like growth factor-1 treatment on body composition and bone mineral density of C57BL/6 mice: implications for cancer prevention. AN - 68006812; 15999532 AB - Calorie restriction (CR) inhibits carcinogenesis and delays aging. Some anti-carcinogenic effects of CR are mediated by decreased circulating insulin-like growth factor-1 (IGF-1); however, IGF-1 also plays an important role in regulating growth and bone density. We quantified tradeoffs involving the CR/IGF-1 axis in C57BL/6 mice by examining body composition and bone characteristics in ad libitum fed, 20, 30 or 40% CR mice that received placebo or recombinant murine IGF-1 delivered with a time-release pellet. After 26 days, carcasses were scanned with a PIXImus II dual-energy X-ray absorptiometer. CR reduced body weight and percent body fat and had non-linear effects on bone density. IGF-1 restored bone density to control levels or greater in the CR mice. Cancer prevention efforts based on CR and down-regulation of the IGF-1 pathway will require consideration of deleterious effects on bone. JF - In vivo (Athens, Greece) AU - Berrigan, David AU - Lavigne, Jackie A AU - Perkins, Susan N AU - Nagy, Tim R AU - Barrett, J Carl AU - Hursting, Stephen D AD - Applied Research Program. Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, MD 20892, USA. berrigad@mail.nih.gov PY - 2005 SP - 667 EP - 674 VL - 19 IS - 4 SN - 0258-851X, 0258-851X KW - Insulin-Like Growth Factor I KW - 67763-96-6 KW - Index Medicus KW - Phenotype KW - Animals KW - Down-Regulation KW - Dose-Response Relationship, Drug KW - Mice, Inbred C57BL KW - Absorptiometry, Photon KW - Energy Intake KW - Mice KW - Female KW - Bone Density -- drug effects KW - Bone and Bones -- drug effects KW - Insulin-Like Growth Factor I -- analysis KW - Caloric Restriction KW - Bone and Bones -- diagnostic imaging KW - Bone Density -- physiology KW - Neoplasms -- prevention & control KW - Bone and Bones -- metabolism KW - Insulin-Like Growth Factor I -- pharmacology KW - Body Composition -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68006812?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=In+vivo+%28Athens%2C+Greece%29&rft.atitle=Phenotypic+effects+of+calorie+restriction+and+insulin-like+growth+factor-1+treatment+on+body+composition+and+bone+mineral+density+of+C57BL%2F6+mice%3A+implications+for+cancer+prevention.&rft.au=Berrigan%2C+David%3BLavigne%2C+Jackie+A%3BPerkins%2C+Susan+N%3BNagy%2C+Tim+R%3BBarrett%2C+J+Carl%3BHursting%2C+Stephen+D&rft.aulast=Berrigan&rft.aufirst=David&rft.date=2005-07-01&rft.volume=19&rft.issue=4&rft.spage=667&rft.isbn=&rft.btitle=&rft.title=In+vivo+%28Athens%2C+Greece%29&rft.issn=0258851X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-30 N1 - Date created - 2005-07-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Development of a food frequency questionnaire module and databases for compounds in cooked and processed meats. AN - 67999624; 15986387 AB - There is ample evidence from basic research and animal carcinogenicity studies that heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) are mutagens and carcinogens. However, there was a paucity of human data due to a lack of appropriate investigative tools. We developed the first validated cooked meat module within a food frequency questionnaire (FFQ) in the United States of America and created databases to be used in conjunction with this FFQ to estimate intake of HCAs and benzo[a]pyrene, a marker of PAHs. It became clear that other aspects of meat may also contribute to carcinogenesis; in particular, we are pursuing two additional areas: processed meat and iron exposure in relation to cancer risk. To investigate these hypotheses, we have expanded the cooked meat module to include detailed information on processed meats and fish. In addition, we are developing two databases, one for total iron and heme iron in cooked meat and the other for nitrite, nitrate, and N-nitroso compounds in processed meats. In this report, we will outline the methods used to develop the meat questionnaires, the databases, a software package for generating the intake values, and the methods used to generate nutritional data from nationally representative samples. JF - Molecular nutrition & food research AU - Sinha, Rashmi AU - Cross, Amanda AU - Curtin, Jane AU - Zimmerman, Thea AU - McNutt, Susanne AU - Risch, Adam AU - Holden, Joanne AD - Nutrition Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20892-7273, USA. sinhar@nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 648 EP - 655 VL - 49 IS - 7 SN - 1613-4125, 1613-4125 KW - Nitrates KW - 0 KW - Nitrites KW - Nitroso Compounds KW - Benzo(a)pyrene KW - 3417WMA06D KW - Heme KW - 42VZT0U6YR KW - Iron KW - E1UOL152H7 KW - Index Medicus KW - Nitrites -- analysis KW - Iron -- analysis KW - Epidemiology KW - Benzo(a)pyrene -- analysis KW - Humans KW - Heme -- chemistry KW - Nitroso Compounds -- analysis KW - Diet KW - Nitrates -- analysis KW - Hot Temperature KW - Diet Records KW - Databases as Topic KW - Surveys and Questionnaires KW - Food Handling KW - Meat -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67999624?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+nutrition+%26+food+research&rft.atitle=Development+of+a+food+frequency+questionnaire+module+and+databases+for+compounds+in+cooked+and+processed+meats.&rft.au=Sinha%2C+Rashmi%3BCross%2C+Amanda%3BCurtin%2C+Jane%3BZimmerman%2C+Thea%3BMcNutt%2C+Susanne%3BRisch%2C+Adam%3BHolden%2C+Joanne&rft.aulast=Sinha&rft.aufirst=Rashmi&rft.date=2005-07-01&rft.volume=49&rft.issue=7&rft.spage=648&rft.isbn=&rft.btitle=&rft.title=Molecular+nutrition+%26+food+research&rft.issn=16134125&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-26 N1 - Date created - 2005-07-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - STRA13 expression and subcellular localisation in normal and tumour tissues: implications for use as a diagnostic and differentiation marker. AN - 67999272; 15994878 AB - STRA13 is a bHLH transcription factor that plays a crucial role in cell differentiation, proliferation, apoptosis, and response to hypoxia. To assess STRA13 involvement in carcinogenesis and evaluate its diagnostic value. A comprehensive analysis was undertaken of the endogenous protein expression in 389 normal and corresponding malignant specimens, using newly generated polyclonal antibodies. STRA13 was commonly expressed in epithelial cells of normal and neoplastic tissues where it was confined mostly to the nucleus. Intense cytoplasmic STRA13 immunoreactivity was characteristic of myoepithelial and differentiated squamous epithelial cells of all organ sites and their neoplastic counterparts, suggesting application of STRA13 as a myoepithelial cell marker. A distinctive apical granular cytoplasmic staining pattern observed in the pancreas and large intestine was retained in corresponding metastatic carcinomas, providing for identification of the primary sites of these disseminating tumours. In less differentiated tumours there was a tendency to lose the cytoplasmic staining or to switch to nuclear STRA13 staining. Analysis of STRA13, HIF-1alpha, and CAIX expression patterns in a large set of various tumours substantiated the association of STRA13 with HIF-1alpha expression and hypoxia in vivo. Investigation of the molecular mechanisms of STRA13 nucleo-cytoplasmic shuttling suggested that STRA13 employs nuclear import/export that utilises the NLS/NES motifs situated within the N-terminus and in the middle of the protein. STRA13 may serve as a marker for myoepithelial cells, for the degree of tumour differentiation, and for identification of the primary site of certain metastatic tumours. In combination with CAIX and CAXII markers, it may lead to a more accurate classification of all renal carcinomas. JF - Journal of medical genetics AU - Ivanova, A AU - Liao, S-Y AU - Lerman, M I AU - Ivanov, S AU - Stanbridge, E J AD - Laboratory of Immunobiology, Basic Research Program, SAIC-Frederick Inc, NCI-Frederick, Maryland, USA. ivanova@mail.ncifcrf.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 565 EP - 576 VL - 42 IS - 7 KW - Antigens, Differentiation KW - 0 KW - Antigens, Neoplasm KW - BHLHE40 protein, human KW - Basic Helix-Loop-Helix Transcription Factors KW - Biomarkers, Tumor KW - Homeodomain Proteins KW - CA9 protein, human KW - EC 4.2.1.1 KW - Carbonic Anhydrase IX KW - Carbonic Anhydrases KW - Index Medicus KW - Cell Nucleus -- metabolism KW - Antigens, Neoplasm -- biosynthesis KW - Humans KW - Kidney Neoplasms -- diagnosis KW - Cell Differentiation KW - Cell Line, Tumor KW - Kidney Neoplasms -- classification KW - Antibody Specificity KW - Carbonic Anhydrases -- biosynthesis KW - Cytoplasm -- metabolism KW - Kidney Neoplasms -- metabolism KW - Immunohistochemistry KW - Cell Line KW - Epithelial Cells -- metabolism KW - Neoplasms -- diagnosis KW - Homeodomain Proteins -- biosynthesis KW - Neoplasms -- classification KW - Basic Helix-Loop-Helix Transcription Factors -- biosynthesis KW - Epithelial Cells -- pathology KW - Antigens, Differentiation -- biosynthesis KW - Biomarkers, Tumor -- biosynthesis KW - Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67999272?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medical+genetics&rft.atitle=STRA13+expression+and+subcellular+localisation+in+normal+and+tumour+tissues%3A+implications+for+use+as+a+diagnostic+and+differentiation+marker.&rft.au=Ivanova%2C+A%3BLiao%2C+S-Y%3BLerman%2C+M+I%3BIvanov%2C+S%3BStanbridge%2C+E+J&rft.aulast=Ivanova&rft.aufirst=A&rft.date=2005-07-01&rft.volume=42&rft.issue=7&rft.spage=565&rft.isbn=&rft.btitle=&rft.title=Journal+of+medical+genetics&rft.issn=1468-6244&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-18 N1 - Date created - 2005-07-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4058-63 [10737769] Curr Opin Cell Biol. 1999 Apr;11(2):241-7 [10209150] Am J Pathol. 2001 Mar;158(3):905-19 [11238039] J Biol Chem. 2001 May 4;276(18):15306-15 [11278694] Cancer Res. 2001 Jul 1;61(13):5262-7 [11431368] Cancer Res. 2001 Sep 1;61(17):6394-9 [11522632] J Biol Chem. 2001 Sep 14;276(37):35042-8 [11470791] Traffic. 2001 Oct;2(10):684-9 [11576444] Nat Immunol. 2001 Nov;2(11):1040-7 [11668339] Cancer Res. 2001 Nov 1;61(21):7992-8 [11691824] Dev Cell. 2002 Mar;2(3):331-41 [11879638] Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2848-53 [11880636] J Biol Chem. 2002 Sep 13;277(37):34471-9 [12089160] Nature. 2002 Oct 24;419(6909):841-4 [12397359] J Biol Chem. 2002 Dec 20;277(51):50112-20 [12384505] J Pathol. 2003 Jun;200(2):222-8 [12754744] Br J Cancer. 2003 Jul 7;89(1):2-7 [12838292] Br J Cancer. 2003 Oct 6;89(7):1290-7 [14520462] Eur J Immunol. 2004 May;34(5):1322-32 [15114665] J Pathol. 2004 Jul;203(3):808-13 [15221940] J Mol Biol. 2004 Jul 16;340(4):641-53 [15223310] Br J Cancer. 2004 Aug 31;91(5):954-8 [15328513] Am J Pathol. 1994 Sep;145(3):598-609 [8080042] Cancer Epidemiol Biomarkers Prev. 1996 Jul;5(7):549-57 [8827360] Genes Dev. 1997 Aug 15;11(16):2052-65 [9284045] Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12596-601 [9770531] Clin Cancer Res. 1997 Nov;3(11):1949-58 [9815584] Cancer Res. 2000 Dec 15;60(24):7075-83 [11156414] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Exposure to indoor allergens in day-care facilities: results from 2 North Carolina counties. AN - 67993707; 15990786 AB - With 63% of US children under 5 years of age in regular child care, day-care facilities could be an important source of exposure to indoor allergens. This study examined levels of 7 indoor allergens in 89 day-care facilities in 2 North Carolina counties. At each facility, a questionnaire was administered, observations were made, and vacuumed dust samples were collected from carpeted and noncarpeted areas of one room. Allergen concentrations were measured with antibody-based ELISAs. Each allergen was detected in a majority of facilities (52% to 100%). Geometric mean concentrations were 5.19 mug/g for Alternaria alternata , 2.06 mug/g for Can f 1, 1.43 microg/g for Fel d 1, 0.21 U/g for Bla g 1, 0.20 microg/g for Der p 1, 0.10 microg/g for Der f 1, and 0.01 microg/g for Mus m 1. Concentrations for 5 of the 7 allergens were not statistically different from concentrations found in southern US homes sampled in the National Survey of Lead and Allergens in Housing. In rooms with carpet and hard-surfaced flooring, levels of A alternata , Can f 1, Der f 1, Der p 1, and Fel d 1 were statistically higher on carpet. In this survey of day-care facilities in North Carolina, detectable levels of indoor allergens were commonly found. For many young children and day-care staff, day-care facilities might be a source of clinically relevant exposures to indoor allergens. JF - The Journal of allergy and clinical immunology AU - Arbes, Samuel J AU - Sever, Michelle AU - Mehta, Jigna AU - Collette, Nicholas AU - Thomas, Brittany AU - Zeldin, Darryl C AD - Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, MD, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 133 EP - 139 VL - 116 IS - 1 SN - 0091-6749, 0091-6749 KW - Allergens KW - 0 KW - Antigens, Dermatophagoides KW - Antigens, Plant KW - Arthropod Proteins KW - allergen Bla g 1 KW - Cysteine Endopeptidases KW - EC 3.4.22.- KW - Dermatophagoides farinae antigen f 1 KW - Dermatophagoides pteronyssinus antigen p 1 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Humans KW - Pyroglyphidae -- immunology KW - Mice KW - Child, Preschool KW - Cockroaches KW - Floors and Floorcoverings KW - North Carolina KW - Cats KW - Dogs KW - Antigens, Dermatophagoides -- analysis KW - Enzyme-Linked Immunosorbent Assay KW - Child Day Care Centers KW - Air Pollution, Indoor KW - Allergens -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67993707?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+allergy+and+clinical+immunology&rft.atitle=Exposure+to+indoor+allergens+in+day-care+facilities%3A+results+from+2+North+Carolina+counties.&rft.au=Arbes%2C+Samuel+J%3BSever%2C+Michelle%3BMehta%2C+Jigna%3BCollette%2C+Nicholas%3BThomas%2C+Brittany%3BZeldin%2C+Darryl+C&rft.aulast=Arbes&rft.aufirst=Samuel&rft.date=2005-07-01&rft.volume=116&rft.issue=1&rft.spage=133&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+allergy+and+clinical+immunology&rft.issn=00916749&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-09 N1 - Date created - 2005-07-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The mechanism by which ethanol inhibits rat P2X4 receptors is altered by mutation of histidine 241. AN - 67991882; 15765101 AB - 1. We investigated ethanol inhibition of the rat P2X(4) receptor and the contribution of the three histidine residues in the extracellular loop of this receptor to ethanol inhibition of receptor function, using site-directed mutagenesis and electrophysiological characterization of recombinant receptors. 2. In the wild-type receptor, 50, 200 and 500 mM ethanol increasingly shifted the ATP concentration-response curve to the right in a parallel manner, increasing the EC(50) value without affecting E(max). However, 750 or 900 mM ethanol did not produce a further increase in the EC(50) value of the ATP concentration-response curve, suggesting that this inhibition is not competitive. 3. The P2X(4) receptor mutations H140A and H286A did not significantly alter ethanol inhibition of ATP-activated current. By contrast, the mutation H241A changed the mechanism by which ethanol inhibits receptor function; viz., ethanol inhibition was not associated with an increased EC(50) value of the ATP concentration-response curve, instead, ethanol decreased the maximal response to ATP without affecting the EC(50) value of the ATP concentration-response curve. 4. Ethanol inhibition of the H241A mutant was voltage independent between -60 and +20 mV and ethanol did not alter the reversal potential of ATP-activated current. In addition, ethanol decreased the desensitization rate of the H241A-mediated current. 5. The purinoceptor antagonists, suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), did not alter the magnitude of ethanol inhibition of ATP-activated current in the H241A mutant. 6. The results suggest that ethanol inhibits the wild-type rat P2X(4) receptor by an allosteric action to increase the EC(50) value of the ATP concentration-response curve, the P2X(4) receptor mutation H241A alters the mechanism by which ethanol inhibits P2X(4) receptor function, and ethanol and PPADS or suramin appear to inhibit H241A-mutated receptors at independent sites. JF - British journal of pharmacology AU - Xiong, Keming AU - Hu, Xiang-Qun AU - Stewart, Randall R AU - Weight, Forrest F AU - Li, Chaoying AD - Laboratory of Molecular and Cellular Neurobiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892-8115, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 576 EP - 586 VL - 145 IS - 5 SN - 0007-1188, 0007-1188 KW - Central Nervous System Depressants KW - 0 KW - P2RX4 protein, human KW - P2rx4 protein, rat KW - Purinergic P2 Receptor Antagonists KW - Receptors, Purinergic P2 KW - Receptors, Purinergic P2X4 KW - Recombinant Proteins KW - Ethanol KW - 3K9958V90M KW - Histidine KW - 4QD397987E KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Index Medicus KW - Rats KW - Mutagenesis, Site-Directed KW - Animals KW - Kidney -- metabolism KW - Patch-Clamp Techniques KW - Humans KW - Oocytes -- drug effects KW - Kidney -- drug effects KW - Xenopus KW - Membrane Potentials -- drug effects KW - Recombinant Proteins -- chemistry KW - Adenosine Triphosphate -- pharmacology KW - Receptors, Purinergic P2 -- genetics KW - Histidine -- physiology KW - Central Nervous System Depressants -- pharmacology KW - Mutation -- physiology KW - Ethanol -- pharmacology KW - Histidine -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67991882?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+journal+of+pharmacology&rft.atitle=The+mechanism+by+which+ethanol+inhibits+rat+P2X4+receptors+is+altered+by+mutation+of+histidine+241.&rft.au=Xiong%2C+Keming%3BHu%2C+Xiang-Qun%3BStewart%2C+Randall+R%3BWeight%2C+Forrest+F%3BLi%2C+Chaoying&rft.aulast=Xiong&rft.aufirst=Keming&rft.date=2005-07-01&rft.volume=145&rft.issue=5&rft.spage=576&rft.isbn=&rft.btitle=&rft.title=British+journal+of+pharmacology&rft.issn=00071188&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-31 N1 - Date created - 2005-07-01 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Neurosci Lett. 2000 Feb 4;279(3):165-8 [10688055] Eur J Neurosci. 1998 Dec;10(12):3898-902 [9875366] Br J Pharmacol. 2000 Jul;130(6):1394-8 [10903981] Neurosci Lett. 2000 Dec 8;295(3):77-80 [11090978] J Neurosci. 2001 Sep 1;21(17):6522-31 [11517241] Alcohol Clin Exp Res. 2002 Jun;26(6):773-8 [12068244] J Physiol. 2002 Jul 15;542(Pt 2):529-36 [12122150] Physiol Rev. 2002 Oct;82(4):1013-67 [12270951] J Biol Chem. 2003 Sep 19;278(38):36777-85 [12819199] Neurosci Lett. 2004 Jul 29;365(3):195-9 [15246547] J Neurosci. 2004 Aug 4;24(31):6968-78 [15295032] J Neurosci. 2004 Aug 18;24(33):7378-86 [15317863] Neurosci Lett. 2004 Sep 2;367(2):197-200 [15331152] J Physiol. 1972 May;223(1):151-71 [4537943] Am J Physiol. 1978 Aug;235(2):E97-102 [686171] Pflugers Arch. 1981 Aug;391(2):85-100 [6270629] Br J Pharmacol. 1987 Jul;91(3):601-8 [3038246] Nature. 1992 Jun 11;357(6378):503-5 [1351659] Nature. 1992 Sep 10;359(6391):144-7 [1381811] Br J Pharmacol. 1992 Aug;106(4):762-3 [1327385] Mol Pharmacol. 1993 Oct;44(4):871-5 [8232236] Nature. 1993 Dec 9;366(6455):510-1 [7504782] Mol Pharmacol. 1994 Feb;45(2):324-9 [8114679] J Neurosci. 1995 May;15(5 Pt 1):3357-65 [7751915] EMBO J. 1996 Jan 2;15(1):55-62 [8598206] J Neurosci. 1996 Apr 15;16(8):2495-507 [8786426] Neurosci Lett. 1996 Jul 19;212(3):212-4 [8843110] Brain Res. 1996 Nov 4;738(2):249-56 [8955520] J Neurosci. 1997 Jul 15;17(14):5297-304 [9204914] J Neurophysiol. 1997 Jun;77(6):3391-5 [9212284] Nature. 1997 Oct 16;389(6652):749-53 [9338789] Br J Pharmacol. 1997 Oct;122(3):423-30 [9351497] Br J Pharmacol. 1997 Nov;122(6):1035-42 [9401766] J Physiol. 1997 Dec 15;505 ( Pt 3):641-53 [9457642] Br J Pharmacol. 1998 Jan;123(1):1-3 [9484847] Mol Pharmacol. 1998 Aug;54(2):372-8 [9687579] J Neurosci. 1999 Jan 15;19(2):737-46 [9880594] J Gen Physiol. 1999 May;113(5):695-720 [10228183] Neurochem Int. 1999 Aug;35(2):143-52 [10405998] Br J Pharmacol Chemother. 1956 Dec;11(4):379-93 [13383117] Brain Res. 2000 Sep 22;877(2):245-50 [10986338] FEBS Lett. 1998 Aug 28;434(1-2):61-5 [9738452] Pharmacol Rev. 1998 Sep;50(3):413-92 [9755289] J Neurosci. 2000 Mar 15;20(6):2121-30 [10704486] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - MPP+-induced COX-2 activation and subsequent dopaminergic neurodegeneration. AN - 67986974; 15845609 AB - The importance of cyclooxygenase-2 (COX-2) in mediating Parkinson's disease (PD) was suggested in reports, indicating that COX-2 selective inhibitors or genetic knockout reduce 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic (DA) neurotoxicity in a mouse model of PD. However, cell types and mechanisms underlying the activation of COX-2 have not been clearly elucidated in these animal studies. Using primary neuron-glia cultures, we aimed to determine 1) whether microglia participate in 1-methyl-4-phenylpryridinium (MPP)-induced COX-2 activation and 2) whether the activation of COX-2 contributes to subsequent neurotoxicity. MPP, in a concentration-dependent manner, increased prostaglandin E2 (PGE2) production in mixed neuron-microglia cultures but not in enriched neuron, microglia, or astroglia cultures nor in mixed neuron-astroglia cultures. MPP-induced PGE2 increase was completely abolished by treatment with DuP697, a COX-2 selective inhibitor. DuP697 also significantly reduced MPP-induced DA neurotoxicity as determined by DA uptake assay. Immunocytochemistry and confocal microscopy studies showed enhanced COX-2 expression in both microglia and neurons after MPP treatment. However, neuronal increase in COX-2 expression was not totally dependent on the production of PGE2 from microglia, since microglia deficient in COX-2 only attenuated, but did not completely block, MPP-increased PGE2 production in mixed neuron-microglia cultures, suggesting that part of PGE2 production was originated from neurons. Together, these results indicate that MPP-induced COX-2 expression and subsequent PGE2 production depend on interactions between neurons and microglia. Microgliosis may also be responsible for the COX-2 activation in neurons, leading to the enhanced DA neurotoxicity, which, in turn, reinforces microgliosis. Thus inhibition of microgliosis and COX-2 activity may stop this vicious circle and be valuable strategies in PD therapy. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Wang, Tongguang AU - Pei, Zhong AU - Zhang, Wei AU - Liu, Bin AU - Langenbach, Robert AU - Lee, Christopher AU - Wilson, Belinda AU - Reece, Jeffrey M AU - Miller, David S AU - Hong, Jau-Shyong AD - Neuropharmacology Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1134 EP - 1136 VL - 19 IS - 9 KW - Cyclooxygenase 2 Inhibitors KW - 0 KW - Cyclooxygenase 2 KW - EC 1.14.99.1 KW - Dinoprostone KW - K7Q1JQR04M KW - 1-Methyl-4-phenylpyridinium KW - R865A5OY8J KW - Index Medicus KW - Animals KW - Dinoprostone -- biosynthesis KW - Cells, Cultured KW - Mice, Inbred C57BL KW - Enzyme Activation -- drug effects KW - Cyclooxygenase 2 Inhibitors -- pharmacology KW - Mice KW - Female KW - Cyclooxygenase 2 -- physiology KW - Microglia -- enzymology KW - 1-Methyl-4-phenylpyridinium -- toxicity KW - Microglia -- drug effects KW - MPTP Poisoning -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67986974?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=MPP%2B-induced+COX-2+activation+and+subsequent+dopaminergic+neurodegeneration.&rft.au=Wang%2C+Tongguang%3BPei%2C+Zhong%3BZhang%2C+Wei%3BLiu%2C+Bin%3BLangenbach%2C+Robert%3BLee%2C+Christopher%3BWilson%2C+Belinda%3BReece%2C+Jeffrey+M%3BMiller%2C+David+S%3BHong%2C+Jau-Shyong&rft.aulast=Wang&rft.aufirst=Tongguang&rft.date=2005-07-01&rft.volume=19&rft.issue=9&rft.spage=1134&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-23 N1 - Date created - 2005-06-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Engraftment syndrome after nonmyeloablative allogeneic hematopoietic stem cell transplantation: incidence and effects on survival. AN - 67974963; 15983554 AB - Engraftment syndrome (ES) encompasses a constellation of symptoms that occur during neutrophil recovery after both autologous and allogeneic hematopoietic stem cell transplantation (HCT). Although it is well characterized after conventional myeloablative procedures, limited data exist on this complication after nonmyeloablative allogeneic HCT. The clinical manifestations, incidence, and risk factors associated with ES were investigated in a consecutive series of patients undergoing cyclophosphamide/fludarabine-based nonmyeloablative allogeneic HCT from a related HLA-compatible donor. Fifteen (10%) of 149 patients (median age, 53 years; range, 27-66 years) developed ES; the onset of symptoms occurred at a median of 10 days (range, 3-14 days), and they consisted of fever (100%), cough (53%), diffuse pulmonary infiltrates (100%), rash (13%), and room air hypoxia (87%). ES was more likely to develop in patients who received empiric amphotericin formulations after transplant conditioning (Fisher exact test; P=.007). In a multivariate analysis, older patient age, female sex, and treatment with amphotericin were predictors for the development of ES. Intravenous methylprednisolone led to the rapid resolution of ES; however, transplant-related mortality was significantly higher (cumulative incidence, 49% versus 16%; P=.0005), and median survival was significantly shorter (168 versus 418 days; P=.005) in patients with ES compared with non-ES patients. In conclusion, ES occurs commonly after cyclophosphamide/fludarabine-based nonmyeloablative transplantation and responds rapidly to corticosteroid treatment, but it is associated with a higher risk of nonrelapse mortality and with shorter overall survival. JF - Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation AU - Gorak, Edward AU - Geller, Nancy AU - Srinivasan, Ramaprasad AU - Espinoza-Delgado, Igor AU - Donohue, Teresa AU - Barrett, A John AU - Suffredini, Anthony AU - Childs, Richard AD - Walter Reed Army Medical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 542 EP - 550 VL - 11 IS - 7 SN - 1083-8791, 1083-8791 KW - Immunosuppressive Agents KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Vidarabine KW - FA2DM6879K KW - fludarabine KW - P2K93U8740 KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Vidarabine -- analogs & derivatives KW - Age Factors KW - Disease-Free Survival KW - Sex Factors KW - Humans KW - Aged KW - Vidarabine -- administration & dosage KW - Neoplasms -- therapy KW - Neoplasms -- pathology KW - Syndrome KW - Risk Factors KW - Adult KW - Incidence KW - Middle Aged KW - Female KW - Male KW - Immunosuppressive Agents -- administration & dosage KW - Neutrophils -- pathology KW - Hematopoietic Stem Cell Transplantation -- mortality KW - Transplantation Conditioning -- methods KW - Myelopoiesis -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67974963?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biology+of+blood+and+marrow+transplantation+%3A+journal+of+the+American+Society+for+Blood+and+Marrow+Transplantation&rft.atitle=Engraftment+syndrome+after+nonmyeloablative+allogeneic+hematopoietic+stem+cell+transplantation%3A+incidence+and+effects+on+survival.&rft.au=Gorak%2C+Edward%3BGeller%2C+Nancy%3BSrinivasan%2C+Ramaprasad%3BEspinoza-Delgado%2C+Igor%3BDonohue%2C+Teresa%3BBarrett%2C+A+John%3BSuffredini%2C+Anthony%3BChilds%2C+Richard&rft.aulast=Gorak&rft.aufirst=Edward&rft.date=2005-07-01&rft.volume=11&rft.issue=7&rft.spage=542&rft.isbn=&rft.btitle=&rft.title=Biology+of+blood+and+marrow+transplantation+%3A+journal+of+the+American+Society+for+Blood+and+Marrow+Transplantation&rft.issn=10838791&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-28 N1 - Date created - 2005-06-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural determinants of factor IX(a) binding in nitrophorin 2, a lipocalin inhibitor of the intrinsic coagulation pathway. AN - 67973205; 15866866 AB - Nitrophorin 2 (NP2) is a salivary lipocalin from Rhodnius prolixus that binds with coagulation factors IX (fIX) and IXa (fIXa). Binding of NP2 with fIXa results in potent inhibition of the intrinsic factor Xase complex. A panel of site-directed surface mutants of NP2 was generated to locate determinants of high affinity fIX(a) binding. The locations of the mutations were based on comparisons with the related, but less potent, inhibitor nitrophorin 3 (NP3). Three point mutants (K21A, K92A, and V94A) were found that clearly reduced the inhibitory potency as measured by the activity of a reconstituted factor Xase system. Binding of NP2 with fIXa and fIX as measured by surface plasmon resonance and isothermal titration calorimetry was reduced in a similar manner. Of the three mutants, two (K92A and V94A) were located on the loop connecting beta-strands E and F of the lipocalin beta-barrel. The largest changes were seen with the K92A mutation, which lies at the apex of the loop, with a smaller effect being seen with mutation of Val(94). Combination of four E-F loop mutations (K92A, A93K, V94A, E97A) in a single mutant reduced the inhibitory potency and binding to levels similar to those seen with NP3 without affecting heme or histamine binding. JF - The Journal of biological chemistry AU - Gudderra, Nanda P AU - Ribeiro, José M C AU - Andersen, John F AD - Laboratory of Malaria and Vector Research NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 25022 EP - 25028 VL - 280 IS - 26 SN - 0021-9258, 0021-9258 KW - Carrier Proteins KW - 0 KW - Hemeproteins KW - LCN1 protein, human KW - Lipocalin 1 KW - Salivary Proteins and Peptides KW - nitrophorin KW - Heme KW - 42VZT0U6YR KW - Histamine KW - 820484N8I3 KW - Factor IX KW - 9001-28-9 KW - Factor IXa KW - EC 3.4.21.22 KW - Index Medicus KW - Animals KW - Models, Molecular KW - Dose-Response Relationship, Drug KW - Humans KW - Surface Plasmon Resonance KW - Factor IX -- chemistry KW - Amino Acid Sequence KW - Protein Binding KW - Mutagenesis, Site-Directed KW - Heme -- chemistry KW - Kinetics KW - Point Mutation KW - Molecular Sequence Data KW - Calorimetry KW - Models, Chemical KW - Histamine -- chemistry KW - Cell Membrane -- metabolism KW - Inhibitory Concentration 50 KW - Sequence Homology, Amino Acid KW - Time Factors KW - Mutation KW - Salivary Proteins and Peptides -- chemistry KW - Rhodnius -- metabolism KW - Carrier Proteins -- chemistry KW - Factor IXa -- chemistry KW - Hemeproteins -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67973205?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Structural+determinants+of+factor+IX%28a%29+binding+in+nitrophorin+2%2C+a+lipocalin+inhibitor+of+the+intrinsic+coagulation+pathway.&rft.au=Gudderra%2C+Nanda+P%3BRibeiro%2C+Jos%C3%A9+M+C%3BAndersen%2C+John+F&rft.aulast=Gudderra&rft.aufirst=Nanda&rft.date=2005-07-01&rft.volume=280&rft.issue=26&rft.spage=25022&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-06-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An overview of phenserine tartrate, a novel acetylcholinesterase inhibitor for the treatment of Alzheimer's disease. AN - 67964318; 15974893 AB - Existing cholinesterase (ChE) inhibitor therapies for Alzheimer's disease (AD), while effective in improving cognitive, behavioral and functional impairments, do not alter disease progression. Novel drug design studies have focused on the classical ChE inhibitor, (-)-physostigmine, producing alterations in chemical composition and three-dimensional structure, which may offer an improved therapeutic index. The phenylcarbamate derivative, (-)-phenserine, is a selective, non-competitive inhibitor of acetylcholinesterase (AChE). In vivo, (-)-phenserine produces rapid, potent, and long-lasting AChE inhibition. As a possible result of its preferential brain selectivity, (-)-phenserine is significantly less toxic than (-)-physostigmine. In studies using the Stone maze paradigm, (-)-phenserine has been shown to improve cognitive performance in both young learning-impaired and elderly rats. In addition to reducing inactivation of acetylcholine in the brain, (-)-phenserine appears to have a second mode of action. Reduced secretion of beta-amyloid (Abeta) has been observed in cell lines exposed to (-)-phenserine, occurring through translational regulation of beta-amyloid precursor protein (beta-APP) mRNA via a non-cholinergic mechanism. These in vitro findings appear to translate in vivo into animal models and humans. In a small study of patients with AD, (-)-phenserine treatment tended to reduce beta-APP and Abeta levels in plasma samples. Clinical studies also reveal that (-)-phenserine (5-10 mg b.i.d.) had a favorable safety and pharmacological profile, produced significant improvements in cognitive function and was well tolerated in patients with AD treated for 12 weeks. Further randomized, double-blind, placebo-controlled Phase III studies assessing the efficacy, safety/tolerability and potential disease-modifying effects of (-)-phenserine in patients with AD are currently ongoing. JF - Current Alzheimer research AU - Greig, Nigel H AU - Sambamurti, Kumar AU - Yu, Qian-sheng AU - Brossi, Arnold AU - Bruinsma, Gosse B AU - Lahiri, Debomoy K AD - Drug Design & Development Section, Laboratory of Neurosciences, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. Greign@grc.nia.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 281 EP - 290 VL - 2 IS - 3 SN - 1567-2050, 1567-2050 KW - Cholinesterase Inhibitors KW - 0 KW - Tartrates KW - phenserine KW - Physostigmine KW - 9U1VM840SP KW - Index Medicus KW - Animals KW - Humans KW - Tartrates -- chemistry KW - Cholinesterase Inhibitors -- adverse effects KW - Cholinesterase Inhibitors -- therapeutic use KW - Cholinesterase Inhibitors -- chemistry KW - Alzheimer Disease -- drug therapy KW - Physostigmine -- analogs & derivatives KW - Cholinesterase Inhibitors -- pharmacokinetics KW - Physostigmine -- chemistry KW - Physostigmine -- therapeutic use KW - Physostigmine -- adverse effects KW - Physostigmine -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67964318?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Alzheimer+research&rft.atitle=An+overview+of+phenserine+tartrate%2C+a+novel+acetylcholinesterase+inhibitor+for+the+treatment+of+Alzheimer%27s+disease.&rft.au=Greig%2C+Nigel+H%3BSambamurti%2C+Kumar%3BYu%2C+Qian-sheng%3BBrossi%2C+Arnold%3BBruinsma%2C+Gosse+B%3BLahiri%2C+Debomoy+K&rft.aulast=Greig&rft.aufirst=Nigel&rft.date=2005-07-01&rft.volume=2&rft.issue=3&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Current+Alzheimer+research&rft.issn=15672050&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-03 N1 - Date created - 2005-06-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tobacco components stimulate Akt-dependent proliferation and NFkappaB-dependent survival in lung cancer cells. AN - 67960528; 15790591 AB - Retrospective studies have shown that patients with tobacco-related cancers who continue to smoke after their diagnoses have lower response rates and shorter median survival compared with patients who stop smoking. To provide insight into the biologic basis for these clinical observations, we tested whether two tobacco components, nicotine or the tobacco-specific carcinogen, 4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone (NNK), could activate the Akt pathway and increase lung cancer cell proliferation and survival. Nicotine or NNK, rapidly and potently, activated Akt in non-small cell lung cancer (NSCLC) or small cell lung cancer (SCLC) cells. Nicotinic activation of Akt increased phosphorylation of multiple downstream substrates of Akt in a time-dependent manner, including GSK-3, FKHR, tuberin, mTOR and S6K1. Since nicotine or NNK bind to cell surface nicotinic acetylcholine receptors (nAchR), we used RT-PCR to assess expression of nine alpha and three beta nAchR subunits in five NSCLC cell lines and two types of primary lung epithelial cells. NSCLC cells express multiple nAchR subunits in a cell line-specific manner. Agonists of alpha3/alpha4 or alpha7 subunits activated Akt in a time-dependent manner, suggesting that tobacco components utilize these subunits to activate Akt. Cellular outcomes after nicotine or NNK administration were also assessed. Nicotine or NNK increased proliferation of NSCLC cells in an Akt-dependent manner that was closely linked with changes in cyclin D1 expression. Despite similar induction of proliferation, only nicotine decreased apoptosis caused by serum deprivation and/or chemotherapy. Protection conferred by nicotine was NFkappaB-dependent. Collectively, these results identify tobacco component-induced, Akt-dependent proliferation and NFkappaB-dependent survival as cellular processes that could underlie the detrimental effects of smoking in cancer patients. JF - Carcinogenesis AU - Tsurutani, Junji AU - Castillo, S Sianna AU - Brognard, John AU - Granville, Courtney A AU - Zhang, Chunyu AU - Gills, Joell J AU - Sayyah, Jacqueline AU - Dennis, Phillip A AD - Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20889, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 1182 EP - 1195 VL - 26 IS - 7 SN - 0143-3334, 0143-3334 KW - Carcinogens KW - 0 KW - Ganglionic Stimulants KW - NF-kappa B KW - Nitrosamines KW - Proto-Oncogene Proteins KW - Nicotine KW - 6M3C89ZY6R KW - 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone KW - 7S395EDO61 KW - AKT1 protein, human KW - EC 2.7.11.1 KW - Protein-Serine-Threonine Kinases KW - Proto-Oncogene Proteins c-akt KW - Index Medicus KW - Tumor Cells, Cultured KW - Phosphorylation KW - Humans KW - Lung -- cytology KW - Carcinoma, Small Cell -- pathology KW - Nitrosamines -- toxicity KW - Protein-Serine-Threonine Kinases -- metabolism KW - Nicotine -- toxicity KW - Proto-Oncogene Proteins -- metabolism KW - Carcinogens -- toxicity KW - Smoking -- adverse effects KW - Cell Proliferation KW - Carcinoma, Non-Small-Cell Lung -- pathology KW - Cell Survival KW - Protein-Serine-Threonine Kinases -- pharmacology KW - Proto-Oncogene Proteins -- pharmacology KW - NF-kappa B -- pharmacology KW - Ganglionic Stimulants -- toxicity KW - Lung Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67960528?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Tobacco+components+stimulate+Akt-dependent+proliferation+and+NFkappaB-dependent+survival+in+lung+cancer+cells.&rft.au=Tsurutani%2C+Junji%3BCastillo%2C+S+Sianna%3BBrognard%2C+John%3BGranville%2C+Courtney+A%3BZhang%2C+Chunyu%3BGills%2C+Joell+J%3BSayyah%2C+Jacqueline%3BDennis%2C+Phillip+A&rft.aulast=Tsurutani&rft.aufirst=Junji&rft.date=2005-07-01&rft.volume=26&rft.issue=7&rft.spage=1182&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-29 N1 - Date created - 2005-06-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Animal models for radiation injury, protection and therapy. AN - 67958137; 15966769 AB - Current events throughout the world underscore the growing threat of different forms of terrorism, including radiological or nuclear attack. Pharmaceutical products and other approaches are needed to protect the civilian population from radiation and to treat those with radiation-induced injuries. In the event of an attack, radiation exposures will be heterogeneous in terms of both dose and quality, depending on the type of device used and each victim's location relative to the radiation source. Therefore, methods are needed to protect against and treat a wide range of early and slowly developing radiation-induced injuries. Equally important is the development of rapid and accurate biodosimetry methods for estimating radiation doses to individuals and guiding clinical treatment decisions. Acute effects of high-dose radiation include hematopoietic cell loss, immune suppression, mucosal damage (gastrointestinal and oral), and potential injury to other sites such as the lung, kidney and central nervous system (CNS). Long-term effects, as a result of both high- and low-dose radiation, include dysfunction or fibrosis in a wide range of organs and tissues and cancer. The availability of appropriate types of animal models, as well as adequate numbers of animals, is likely to be a major bottleneck in the development of new or improved radioprotectors, mitigators and therapeutic agents to prevent or treat radiation injuries and of biodosimetry methods to measure radiation doses to individuals. JF - Radiation research AU - Augustine, Alison Deckhut AU - Gondré-Lewis, Timothy AU - McBride, William AU - Miller, Lara AU - Pellmar, Terry C AU - Rockwell, Sara AD - Division of Allergy, Immunology and Transplantation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-6601, USA. adeckhut@niaid.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 100 EP - 109 VL - 164 IS - 1 SN - 0033-7587, 0033-7587 KW - Radiation-Protective Agents KW - 0 KW - Index Medicus KW - Space life sciences KW - Animals KW - Humans KW - Radiation Injuries -- drug therapy KW - Radiation Protection -- methods KW - Radiation-Protective Agents -- therapeutic use KW - Radiation Injuries -- prevention & control KW - Disease Models, Animal KW - Research Design KW - Drug Design UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67958137?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=proceeding&rft.jtitle=Radiation+research&rft.atitle=Animal+models+for+radiation+injury%2C+protection+and+therapy.&rft.au=Augustine%2C+Alison+Deckhut%3BGondr%C3%A9-Lewis%2C+Timothy%3BMcBride%2C+William%3BMiller%2C+Lara%3BPellmar%2C+Terry+C%3BRockwell%2C+Sara&rft.aulast=Augustine&rft.aufirst=Alison&rft.date=2005-07-01&rft.volume=164&rft.issue=1&rft.spage=100&rft.isbn=&rft.btitle=&rft.title=Radiation+research&rft.issn=00337587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-23 N1 - Date created - 2005-06-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - NUP98-HOXD13 transgenic mice develop a highly penetrant, severe myelodysplastic syndrome that progresses to acute leukemia. AN - 67952480; 15755899 AB - The myelodysplastic syndromes (MDSs) are a group of clonal hematopoietic stem-cell disorders characterized by ineffective hematopoiesis and dysplasia. A wide spectrum of genetic aberrations has been associated with MDS, including chromosomal translocations involving the NUP98 gene. Using a NUP98-HOXD13 fusion gene, we have developed a mouse model that faithfully recapitulates all of the key features of MDS, including peripheral blood cytopenias, bone marrow dysplasia, and apoptosis, and transformation to acute leukemia. The MDS that develops in NUP98-HOXD13 transgenic mice is uniformly fatal. Within 14 months, all of the mice died of either leukemic transformation or severe anemia and leucopenia as a result of progressive MDS. The NUP98-HOXD13 fusion gene inhibits megakaryocytic differentiation and increases apoptosis in the bone marrow, suggesting a mechanism leading to ineffective hematopoiesis in the presence of a hypercellular bone marrow. These mice provide an accurate preclinical model that can be used for the evaluation of MDS therapy and biology. JF - Blood AU - Lin, Ying-Wei AU - Slape, Christopher AU - Zhang, Zhenhua AU - Aplan, Peter D AD - Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20889, USA. Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 287 EP - 295 VL - 106 IS - 1 SN - 0006-4971, 0006-4971 KW - Carcinogens KW - 0 KW - HOXD13 protein, human KW - Homeodomain Proteins KW - Hoxd13 protein, mouse KW - Neoplasm Proteins KW - Nuclear Pore Complex Proteins KW - Transcription Factors KW - myeloid ecotropic viral integration site 1 protein KW - nuclear pore complex protein 98 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Abridged Index Medicus KW - Index Medicus KW - Severity of Illness Index KW - Acute Disease KW - Animals KW - Carcinogens -- pharmacology KW - Humans KW - Disease Models, Animal KW - Mice KW - K562 Cells KW - Mice, Transgenic KW - Translocation, Genetic KW - Phenotype KW - Penetrance KW - Cell Differentiation -- physiology KW - Neoplasm Proteins -- genetics KW - Mice, Inbred C57BL KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Cell Differentiation -- drug effects KW - Cell Transformation, Neoplastic KW - Homeodomain Proteins -- genetics KW - Leukemia -- physiopathology KW - Transcription Factors -- genetics KW - Myelodysplastic Syndromes -- genetics KW - Nuclear Pore Complex Proteins -- genetics KW - Myelodysplastic Syndromes -- physiopathology KW - Leukemia -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67952480?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=NUP98-HOXD13+transgenic+mice+develop+a+highly+penetrant%2C+severe+myelodysplastic+syndrome+that+progresses+to+acute+leukemia.&rft.au=Lin%2C+Ying-Wei%3BSlape%2C+Christopher%3BZhang%2C+Zhenhua%3BAplan%2C+Peter+D&rft.aulast=Lin&rft.aufirst=Ying-Wei&rft.date=2005-07-01&rft.volume=106&rft.issue=1&rft.spage=287&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-06-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 2001 Aug 3;276(31):29526-30 [11395513] Cell Growth Differ. 1999 Sep;10(9):639-54 [10511314] Cancer Res. 2002 Jan 1;62(1):33-7 [11782354] Blood. 2002 May 15;99(10):3857-60 [11986249] Proc Natl Acad Sci U S A. 2002 May 28;99(11):7622-7 [12032333] Blood. 2002 Jul 1;100(1):238-45 [12070033] Blood. 2002 Jul 1;100(1):246-58 [12070034] Cancer Res. 2002 Aug 15;62(16):4571-4 [12183408] Semin Hematol. 2002 Jul;39(3 Suppl 2):18-25 [12214289] Blood. 2002 Oct 1;100(7):2292-302 [12239137] Genes Chromosomes Cancer. 2003 Jan;36(1):107-12 [12461755] J Biol Chem. 2002 Dec 27;277(52):50597-606 [12372823] Leuk Res. 2003 Feb;27(2):95-120 [12526916] Blood. 2003 Jun 1;101(11):4529-38 [12543865] Cancer Res. 2003 Jun 15;63(12):3079-83 [12810632] Hematology Am Soc Hematol Educ Program. 2003;:176-99 [14633782] Int J Hematol. 2004 Feb;79(2):147-51 [15005342] Blood. 2004 May 1;103(9):3265-70 [14684416] Blood. 2004 Jun 15;103(12):4503-10 [15001465] J Clin Invest. 2004 Sep;114(5):713-9 [15343390] Leuk Lymphoma. 2004 Jul;45(7):1341-50 [15359631] Br J Haematol. 1986 Nov;64(3):487-91 [3539173] Blood. 1991 Aug 1;78(3):768-74 [1859889] Cell. 1995 Apr 21;81(2):215-22 [7736573] Blood. 1999 Nov 1;94(9):3258-61 [10556215] Leukemia. 1999 Nov;13(11):1741-8 [10557047] Leukemia. 1999 Dec;13(12):1993-9 [10602420] Cancer Res. 2000 Nov 15;60(22):6227-9 [11103774] Mol Cell Biol. 2001 Jan;21(1):224-34 [11113197] EMBO J. 2001 Feb 1;20(3):350-61 [11157742] Semin Oncol. 1995 Aug;22(4):355-73 [7638633] Nat Genet. 1996 Feb;12(2):149-53 [8563752] Nat Genet. 1996 Feb;12(2):159-67 [8563754] J Cell Biol. 1997 Jan 27;136(2):241-50 [9015297] Blood. 1997 Jun 1;89(11):3936-44 [9166830] Blood. 1998 Jan 15;91(2):419-30 [9427694] EMBO J. 1998 Jul 1;17(13):3714-25 [9649441] Cancer Res. 1998 Oct 1;58(19):4269-73 [9766650] Mol Cell Biol. 1999 Apr;19(4):3051-61 [10082572] Mol Cell Biol. 1999 Jul;19(7):5025-35 [10373552] Blood. 1999 Jul 15;94(2):741-7 [10397741] Blood. 1999 Sep 15;94(6):1855-63 [10477714] Blood. 1999 Sep 15;94(6):2072-9 [10477737] Blood. 2001 Aug 15;98(4):1264-7 [11493482] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Donepezil for dementia in Parkinson's disease: a randomised, double blind, placebo controlled, crossover study. AN - 67949087; 15965198 AB - To study the safety and efficacy of a cholinesterase inhibitor, donepezil hydrochloride, for the treatment of dementia in Parkinson's disease (PD). This was a randomised double blind, placebo controlled, crossover study in 22 subjects with PD and dementia. Participants were randomised to receive either donepezil followed by identical placebo, or placebo followed by donepezil. Donepezil was administered at 5-10 mg/day. Treatment periods were 10 weeks with a washout period of 6 weeks between the two periods. The primary outcome measure was the Alzheimer's Disease Assessment Scale Cognitive Subscale (ADAScog). Donepezil was well tolerated and most adverse events were mild. There was no worsening of PD symptoms as measured by the total or motor sections of the Unified Parkinson's Disease Rating Scale.There was a 1.9 point trend toward better scores on the ADAScog on treatment compared with placebo that was not statistically significant. The secondary cognitive measures showed a statistically significant 2 point benefit on the Mini Mental Status Examination and no change on the Mattis Dementia Rating Scale (MDRS). The Clinical Global Impression of Change (CGI) showed a significant 0.37 point improvement on donepezil. No improvement was observed on the MDRS or the Brief Psychiatric Rating Scale. Carryover between treatment periods was observed but was not statistically significant. Donepezil was well tolerated and did not worsen PD. There may be a modest benefit on aspects of cognitive function. The possible clinical benefit measured by CGI was reflected in only one of the cognitive scales used in this study. JF - Journal of neurology, neurosurgery, and psychiatry AU - Ravina, B AU - Putt, M AU - Siderowf, A AU - Farrar, J T AU - Gillespie, M AU - Crawley, A AU - Fernandez, H H AU - Trieschmann, M M AU - Reichwein, S AU - Simuni, T AD - NINDS, Neuroscience Center Rm 2225, 6001 Executive Blvd, Rockville, MD 20892-9257, USA. ravinab@ninds.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 934 EP - 939 VL - 76 IS - 7 SN - 0022-3050, 0022-3050 KW - Cholinesterase Inhibitors KW - 0 KW - Indans KW - Piperidines KW - donepezil KW - 8SSC91326P KW - Index Medicus KW - Double-Blind Method KW - Aged, 80 and over KW - Humans KW - Treatment Outcome KW - Cross-Over Studies KW - Aged KW - Middle Aged KW - Neuropsychological Tests KW - Male KW - Female KW - Indans -- therapeutic use KW - Cholinesterase Inhibitors -- adverse effects KW - Piperidines -- therapeutic use KW - Cholinesterase Inhibitors -- therapeutic use KW - Dementia -- diagnosis KW - Dementia -- drug therapy KW - Piperidines -- adverse effects KW - Parkinson Disease -- drug therapy KW - Parkinson Disease -- diagnosis KW - Indans -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67949087?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurology%2C+neurosurgery%2C+and+psychiatry&rft.atitle=Donepezil+for+dementia+in+Parkinson%27s+disease%3A+a+randomised%2C+double+blind%2C+placebo+controlled%2C+crossover+study.&rft.au=Ravina%2C+B%3BPutt%2C+M%3BSiderowf%2C+A%3BFarrar%2C+J+T%3BGillespie%2C+M%3BCrawley%2C+A%3BFernandez%2C+H+H%3BTrieschmann%2C+M+M%3BReichwein%2C+S%3BSimuni%2C+T&rft.aulast=Ravina&rft.aufirst=B&rft.date=2005-07-01&rft.volume=76&rft.issue=7&rft.spage=934&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurology%2C+neurosurgery%2C+and+psychiatry&rft.issn=00223050&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-19 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Control Clin Trials. 2002 Apr;23(2):111-26 [11943439] Neurology. 1999 Sep 22;53(5):902-5 [10496243] Ann Neurol. 2002 Jun;51(6):722-9 [12112078] Mov Disord. 2002 Jul;17(4):758-63 [12210871] Clin Neuropharmacol. 2002 Nov-Dec;25(6):296-9 [12469000] Neurology. 2002 Dec 10;59(11):1708-13 [12473757] Mov Disord. 2003 May;18(5):467-86 [12722160] Am J Epidemiol. 2003 Jun 1;157(11):1015-22 [12777365] Dement Geriatr Cogn Disord. 2004;17(1-2):100-8 [14564129] Int J Geriatr Psychiatry. 2004 Jan;19(1):1-8 [14716693] Mov Disord. 2004 Jan;19(1):29-35 [14743357] Parkinsonism Relat Disord. 2004 May;10 Suppl 1:S15-8 [15109582] Lancet. 2004 Jun 26;363(9427):2105-15 [15220031] Mov Disord. 2004 Sep;19(9):1043-9 [15372593] Brain Res. 1982 Jan 28;232(1):129-39 [7055689] J Neurol Sci. 1983 May;59(2):277-89 [6854353] Ann Neurol. 1985 Feb;17(2):163-70 [3883886] J Neurol Neurosurg Psychiatry. 1985 May;48(5):413-21 [3998751] Hosp Pract (Off Ed). 1987 Jan 30;22(1A):99, 103, 106, 110 [3100557] J Neurol Neurosurg Psychiatry. 2002 Jun;72(6):708-12 [12023410] Am J Geriatr Psychiatry. 2000 Spring;8(2):134-40 [10804074] Neurology. 2000 May 23;54(10):1916-21 [10822429] Lancet. 2000 Dec 16;356(9247):2031-6 [11145488] Expert Opin Pharmacother. 1999 Nov;1(1):121-35 [11249555] Adv Neurol. 2001;86:249-55 [11553984] Psychopharmacol Bull. 1988;24(4):627-8 [3249763] Alzheimer Dis Assoc Disord. 1992 Summer;6(2):89-102 [1389084] J Geriatr Psychiatry Neurol. 1995 Jul;8(3):184-8 [7576044] Dementia. 1996 Nov-Dec;7(6):293-303 [8915035] Arch Neurol. 1997 Mar;54(3):260-4 [9074394] Neurology. 1998 Jan;50(1):136-45 [9443470] Arch Intern Med. 1998 May 11;158(9):1021-31 [9588436] Comment In: J Neurol Neurosurg Psychiatry. 2005 Jul;76(7):903-4 [15965192] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In utero exposure to background levels of polychlorinated biphenyls and cognitive functioning among school-age children. AN - 67944158; 15961582 AB - Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants. In utero exposure to background levels of PCBs has been associated with intellectual impairment among children in most, but not all, studies. The authors evaluated prenatal PCB exposure in relation to cognitive test (intelligence quotient (IQ)) scores on the Wechsler Intelligence Scale for Children at age 7 years. Pregnant women were recruited from 12 US study centers from 1959 to 1965, and their children were followed until age 7 years (the Collaborative Perinatal Project). Third trimester serum was analyzed for PCBs in 1997-1999 for 732 women selected at random and for an additional 162 women whose children had either a low or a high IQ score. The PCB-IQ association was examined in multivariate models. Among those in the lowest exposure category ( or =5 microg of PCB/liter), the mean IQ was 97.6 (standard error: 1.2); and overall the increase in IQ per unit increase in PCB level (microg/liter) was 0.22 (95% confidence interval: -0.28, 0.71). In these data, in utero exposure to background levels of PCBs was not associated with lower IQ at age 7 years. JF - American journal of epidemiology AU - Gray, Kimberly A AU - Klebanoff, Mark A AU - Brock, John W AU - Zhou, Haibo AU - Darden, Rebecca AU - Needham, Larry AU - Longnecker, Matthew P AD - Division of Extramural Research and Training, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 17 EP - 26 VL - 162 IS - 1 SN - 0002-9262, 0002-9262 KW - Environmental Pollutants KW - 0 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - United States KW - Pregnancy Trimester, Third KW - Humans KW - Abnormalities, Drug-Induced -- psychology KW - Infant, Newborn KW - Child Development -- drug effects KW - Child KW - Pregnancy KW - Child, Preschool KW - Infant KW - Maternal-Fetal Exchange KW - Adult KW - Cohort Studies KW - Follow-Up Studies KW - Male KW - Female KW - Intelligence Tests KW - Maternal Exposure -- adverse effects KW - Cognition Disorders -- diagnosis KW - Environmental Pollutants -- toxicity KW - Polychlorinated Biphenyls -- toxicity KW - Polychlorinated Biphenyls -- blood KW - Cognition Disorders -- chemically induced KW - Environmental Pollutants -- blood KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67944158?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+epidemiology&rft.atitle=In+utero+exposure+to+background+levels+of+polychlorinated+biphenyls+and+cognitive+functioning+among+school-age+children.&rft.au=Gray%2C+Kimberly+A%3BKlebanoff%2C+Mark+A%3BBrock%2C+John+W%3BZhou%2C+Haibo%3BDarden%2C+Rebecca%3BNeedham%2C+Larry%3BLongnecker%2C+Matthew+P&rft.aulast=Gray&rft.aufirst=Kimberly&rft.date=2005-07-01&rft.volume=162&rft.issue=1&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=American+journal+of+epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-04 N1 - Date created - 2005-06-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Carboxyl tail cysteine mutants of the thyrotropin-releasing hormone receptor type 1 exhibit constitutive signaling: role of palmitoylation. AN - 67941974; 15833733 AB - We studied the role of carboxyl tail cysteine residues and their palmitoylation in constitutive signaling by the thyrotropin-releasing hormone (TRH) receptor type 1 (TRH-R1) in transfected mammalian cells and in Xenopus laevis oocytes. To study palmitoylation, we inserted a factor Xa cleavage site within the third extracellular loop of TRH-R1, added a carboxyl-terminal C9 immunotag and expressed the mutant receptor in Chinese hamster ovary cells. We identified TRH-R1-specific palmitoylation in the transmembrane helix-7/carboxyl-tail receptor fragment mainly at Cys-335 and Cys-337. In contrast to a mutant truncated at Cys-335 that was reported previously to be constitutively active, a receptor truncated at Lys-338 (K338Stop), which preserves Cys-335 and Cys-337, and C337Stop and N336Stop, which preserve Cys-335, did not exhibit increased constitutive signaling. TRH-R1 mutants substituted singly by Gly or Ser at Cys-335 or Cys-337 did not exhibit constitutive signaling. By contrast, substitution of both cysteines (C335G/C337G or C335S/C337S) yielded TRH-R1 mutants that exhibited marked constitutive signaling in mammalian cells. In the oocyte, constitutive signaling by C335G/C337G resulted in homologous (of C335G/C337G) and heterologous (of M1 muscarinic receptor) desensitization. Because both Cys-335 and Cys-337 have to be substituted or deleted for constitutive signaling, we propose that a single palmitoylation site in the proximal carboxyl tail is sufficient to constrain TRH-R1 in an inactive conformation. JF - Molecular pharmacology AU - Du, Dongyi AU - Raaka, Bruce M AU - Grimberg, Hagit AU - Lupu-Meiri, Monica AU - Oron, Yoram AU - Gershengorn, Marvin C AD - Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-8029, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 204 EP - 209 VL - 68 IS - 1 SN - 0026-895X, 0026-895X KW - Peptide Fragments KW - 0 KW - Receptors, Thyrotropin-Releasing Hormone KW - Palmitic Acid KW - 2V16EO95H1 KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Xenopus laevis KW - Animals KW - Humans KW - CHO Cells KW - Female KW - Cell Line KW - Cricetinae KW - Mutagenesis, Site-Directed KW - Peptide Fragments -- genetics KW - Cysteine -- genetics KW - Receptors, Thyrotropin-Releasing Hormone -- genetics KW - Signal Transduction -- genetics KW - Peptide Fragments -- physiology KW - Palmitic Acid -- metabolism KW - Receptors, Thyrotropin-Releasing Hormone -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67941974?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+pharmacology&rft.atitle=Carboxyl+tail+cysteine+mutants+of+the+thyrotropin-releasing+hormone+receptor+type+1+exhibit+constitutive+signaling%3A+role+of+palmitoylation.&rft.au=Du%2C+Dongyi%3BRaaka%2C+Bruce+M%3BGrimberg%2C+Hagit%3BLupu-Meiri%2C+Monica%3BOron%2C+Yoram%3BGershengorn%2C+Marvin+C&rft.aulast=Du&rft.aufirst=Dongyi&rft.date=2005-07-01&rft.volume=68&rft.issue=1&rft.spage=204&rft.isbn=&rft.btitle=&rft.title=Molecular+pharmacology&rft.issn=0026895X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-06-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Proline mutations induce negative-dosage effects on uptake velocity of the dopamine transporter. AN - 67934897; 15953370 AB - Ala and Gly substitutions for Pro 101 (P101) located in transmembrane domain 2 of the dopamine transporter (DAT) abolished transport activity but did not disrupt plasma membrane expression. Due to the high conservation of P101 in all neurotransmitter transporters and the capability of Pro to add flexibility to helices, we hypothesized that P101 contributes to the dynamic feature of substrate translocation. To test this hypothesis, here we analysed transport activity for DAT mutants where this Pro was mutated into different amino acids, including Ser, Val, Leu and Phe. The transmembrane domain 2 helix of P101F, unlike the other mutants, was computationally predicted to have a Van der Waals energy threefold higher than the wild-type helix. P101F mutant expression was consistently disrupted in COS cells. Among all the other mutants that express normally, P101V, with a side-chain size close to that of Pro, restores the transport activity of P101A by sevenfold. Most importantly, P101V, P101L and P101S display negative-dosage effects on dopamine (DA) transport, i.e. the velocity-concentration curve for DA uptake does not show a plateau with increasing [DA] but rather peaks and then goes down. These data support the view that P101 of DAT plays an essential role in DA translocation. JF - Journal of neurochemistry AU - Lin, Zhicheng AU - Uhl, George R AD - Molecular Neurobiology Branch, NIDA-IRP, NIH, Baltimore, MD 21224, USA. zlin@intra.nida.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 276 EP - 287 VL - 94 IS - 1 SN - 0022-3042, 0022-3042 KW - Dopamine Plasma Membrane Transport Proteins KW - 0 KW - Dopamine Uptake Inhibitors KW - Membrane Glycoproteins KW - Membrane Transport Proteins KW - Nerve Tissue Proteins KW - (1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester KW - 50370-56-4 KW - Proline KW - 9DLQ4CIU6V KW - Cocaine KW - I5Y540LHVR KW - Dopamine KW - VTD58H1Z2X KW - Tyramine KW - X8ZC7V0OX3 KW - Index Medicus KW - Animals KW - Tyramine -- metabolism KW - Thermodynamics KW - COS Cells KW - Amino Acid Substitution -- genetics KW - Cercopithecus aethiops KW - Dopamine -- metabolism KW - Dopamine Uptake Inhibitors -- metabolism KW - Proline -- chemistry KW - Membrane Glycoproteins -- chemistry KW - Membrane Transport Proteins -- chemistry KW - Nerve Tissue Proteins -- genetics KW - Proline -- genetics KW - Mutagenesis, Site-Directed KW - Cocaine -- analogs & derivatives KW - Nerve Tissue Proteins -- metabolism KW - Cocaine -- metabolism KW - Nerve Tissue Proteins -- chemistry KW - Membrane Transport Proteins -- genetics KW - Membrane Transport Proteins -- metabolism KW - Membrane Glycoproteins -- genetics KW - Membrane Glycoproteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67934897?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurochemistry&rft.atitle=Proline+mutations+induce+negative-dosage+effects+on+uptake+velocity+of+the+dopamine+transporter.&rft.au=Lin%2C+Zhicheng%3BUhl%2C+George+R&rft.aulast=Lin&rft.aufirst=Zhicheng&rft.date=2005-07-01&rft.volume=94&rft.issue=1&rft.spage=276&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-08 N1 - Date created - 2005-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Differential long-term neuroadaptations of glutamate receptors in the basolateral and central amygdala after withdrawal from cocaine self-administration in rats. AN - 67934548; 15953359 AB - Humans and laboratory animals remain highly vulnerable to relapse to cocaine-seeking after prolonged periods of withdrawal from the drug. It has been hypothesized that this persistent cocaine relapse vulnerability involves drug-induced alterations in glutamatergic synapses within the mesolimbic dopamine reward system. Previous studies have shown that cocaine self-administration induces long-lasting neuroadaptations in glutamate neurons of the ventral tegmental area and nucleus accumbens. Here, we determined the effect of cocaine self-administration and subsequent withdrawal on glutamate receptor expression in the amygdala, a component of the mesolimbic dopamine system that is involved in cocaine seeking and craving induced by drug-associated cues. Rats were trained for 10 days to self-administer intravenous cocaine (6 h/day) or saline (a control condition) and were killed after one or 30 withdrawal days. Basolateral and central amygdala tissues were assayed for protein expression of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor subunits (GluR1 and GluR2) and the NMDA receptor subunits (NR1, NR2A and NR2B). In the basolateral amygdala, GluR1 but not GluR2 levels were increased on days 1 and 30, NR2A levels were increased on day 1, and NR2B levels were decreased on day 30 of withdrawal from cocaine. In the central amygdala, GluR2 but not GluR1 levels were increased on days 1 and 30, NR1 levels were increased on day 30 and NR2A or NR2B levels were not altered after withdrawal from cocaine. These results indicate that cocaine self-administration and subsequent withdrawal induces long-lasting and differential neuroadaptations in basolateral and central amygdala glutamate receptors. JF - Journal of neurochemistry AU - Lu, Lin AU - Dempsey, Jack AU - Shaham, Yavin AU - Hope, Bruce T AD - Behavioral Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, National Institute on Health, Department of Health and Human Services, Baltimore, MD 21224, USA. llu@intra.nida.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 161 EP - 168 VL - 94 IS - 1 SN - 0022-3042, 0022-3042 KW - Receptors, Glutamate KW - 0 KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Rats KW - Animals KW - Time KW - Self Administration KW - Rats, Long-Evans KW - Male KW - Adaptation, Physiological -- drug effects KW - Receptors, Glutamate -- genetics KW - Amygdala -- metabolism KW - Substance Withdrawal Syndrome -- metabolism KW - Adaptation, Physiological -- physiology KW - Substance Withdrawal Syndrome -- genetics KW - Receptors, Glutamate -- physiology KW - Receptors, Glutamate -- biosynthesis KW - Amygdala -- drug effects KW - Cocaine -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67934548?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurochemistry&rft.atitle=Differential+long-term+neuroadaptations+of+glutamate+receptors+in+the+basolateral+and+central+amygdala+after+withdrawal+from+cocaine+self-administration+in+rats.&rft.au=Lu%2C+Lin%3BDempsey%2C+Jack%3BShaham%2C+Yavin%3BHope%2C+Bruce+T&rft.aulast=Lu&rft.aufirst=Lin&rft.date=2005-07-01&rft.volume=94&rft.issue=1&rft.spage=161&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-08 N1 - Date created - 2005-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Enhanced susceptibility of staggerer (RORalphasg/sg) mice to lipopolysaccharide-induced lung inflammation. AN - 67932703; 15778248 AB - The retinoid-related orphan receptor alpha (RORalpha), a member of the ROR subfamily of nuclear receptors, has been implicated in the control of a number of physiological processes, including the regulation of several immune functions. To study the potential role of RORalpha in the regulation of innate immune responses in vivo, we analyzed the induction of airway inflammation in response to lipopolysaccharide (LPS) challenge in wild-type and staggerer (RORalpha(sg/sg)) mice, a natural mutant strain lacking RORalpha expression. Examination of hematoxylin and eosin-stained lung sections showed that RORalpha(sg/sg) mice displayed a higher degree of LPS-induced inflammation than wild-type mice. Bronchoalveolar lavage (BAL) was performed at 3, 16, and 24 h after LPS exposure to monitor the increase in inflammatory cells and the level of several cytokines/chemokines. The increased susceptibility of RORalpha(sg/sg) mice to LPS-induced airway inflammation correlated with a higher number of total cells and neutrophils in BAL fluids from LPS-treated RORalpha(sg/sg) mice compared with those from LPS-treated wild-type mice. In addition, IL-1beta, IL-6, and macrophage inflammatory protein-2 were appreciably more elevated in BAL fluids from LPS-treated RORalpha(sg/sg) mice compared with those from LPS-treated wild-type mice. The enhanced susceptibility of RORalpha(sg/sg) mice appeared not to be due to a repression of IkappaBalpha expression. Our observations indicate that RORalpha(sg/sg) mice are more susceptible to LPS-induced airway inflammation and are in agreement with the hypothesis that RORalpha functions as a negative regulator of LPS-induced inflammatory responses. JF - American journal of physiology. Lung cellular and molecular physiology AU - Stapleton, Cliona M AU - Jaradat, Maisa AU - Dixon, Darlene AU - Kang, Hong Soon AU - Kim, Seong-Chul AU - Liao, Grace AU - Carey, Michelle A AU - Cristiano, Joey AU - Moorman, Michael P AU - Jetten, Anton M AD - Division of Intramural Research, Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - L144 EP - L152 VL - 289 IS - 1 SN - 1040-0605, 1040-0605 KW - Cytokines KW - 0 KW - I-kappa B Proteins KW - Lipopolysaccharides KW - Nuclear Receptor Subfamily 1, Group F, Member 1 KW - Receptors, Cytoplasmic and Nuclear KW - Trans-Activators KW - Index Medicus KW - Animals KW - Cytokines -- biosynthesis KW - I-kappa B Proteins -- metabolism KW - Inflammation -- genetics KW - Mice KW - Neutrophil Infiltration KW - Mice, Knockout KW - Mice, Neurologic Mutants KW - Inflammation -- metabolism KW - Bronchoalveolar Lavage Fluid -- cytology KW - Female KW - Male KW - Inflammation -- pathology KW - Trans-Activators -- metabolism KW - Pneumonia -- chemically induced KW - Trans-Activators -- genetics KW - Receptors, Cytoplasmic and Nuclear -- metabolism KW - Lipopolysaccharides -- toxicity KW - Receptors, Cytoplasmic and Nuclear -- genetics KW - Pneumonia -- pathology KW - Pneumonia -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67932703?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+physiology.+Lung+cellular+and+molecular+physiology&rft.atitle=Enhanced+susceptibility+of+staggerer+%28RORalphasg%2Fsg%29+mice+to+lipopolysaccharide-induced+lung+inflammation.&rft.au=Stapleton%2C+Cliona+M%3BJaradat%2C+Maisa%3BDixon%2C+Darlene%3BKang%2C+Hong+Soon%3BKim%2C+Seong-Chul%3BLiao%2C+Grace%3BCarey%2C+Michelle+A%3BCristiano%2C+Joey%3BMoorman%2C+Michael+P%3BJetten%2C+Anton+M&rft.aulast=Stapleton&rft.aufirst=Cliona&rft.date=2005-07-01&rft.volume=289&rft.issue=1&rft.spage=L144&rft.isbn=&rft.btitle=&rft.title=American+journal+of+physiology.+Lung+cellular+and+molecular+physiology&rft.issn=10400605&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-06-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pilot study of interferon gamma for chronic hepatitis C. AN - 67927268; 15913831 AB - Currently, there are no effective therapies available for patients with chronic hepatitis C who have failed to respond to optimal interferon alfa-based regimens. The aims of this pilot study were to assess the antiviral activity and safety of interferon gamma in chronic hepatitis C. Patients with chronic hepatitis C, genotype 1, who had not responded to or who had relapsed after therapy with interferon alfa and ribavirin were enrolled in a trial of interferon gamma 1b given in doses of 100, 200 or 400 microg subcutaneously three times weekly for 4 weeks. Frequent blood samples were obtained for HCV RNA levels. Fourteen patients were enrolled. Geometric mean HCV RNA levels remained unchanged. Serum aminotransferase levels also did not change, while there were significant decreases in neutrophil counts (-41% from baseline) and hematocrit (-5%). Low grade fever and malaise were common with the first injection of interferon gamma, but no serious side effects were encountered. Although relatively well tolerated, interferon gamma in doses of 100-400 microg thrice weekly had no effect on HCV RNA levels in patients with chronic hepatitis C who had failed to achieve a sustained response to interferon alfa-based therapies. JF - Journal of hepatology AU - Soza, Alejandro AU - Heller, Theo AU - Ghany, Marc AU - Lutchman, Glen AU - Jake Liang, T AU - Germain, June AU - Hsu, Henry H AU - Park, Yoon AU - Hoofnagle, Jay H AD - The Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 67 EP - 71 VL - 43 IS - 1 SN - 0168-8278, 0168-8278 KW - Antiviral Agents KW - 0 KW - Interferon-alpha KW - RNA, Viral KW - Ribavirin KW - 49717AWG6K KW - Interferon-gamma KW - 82115-62-6 KW - Transaminases KW - EC 2.6.1.- KW - Index Medicus KW - Hepacivirus -- physiology KW - Treatment Failure KW - Ribavirin -- therapeutic use KW - Interferon-alpha -- therapeutic use KW - Dose-Response Relationship, Drug KW - Hepacivirus -- genetics KW - Humans KW - Pilot Projects KW - Recurrence KW - Blood Cell Count KW - Genotype KW - Transaminases -- blood KW - Drug Resistance, Viral KW - Middle Aged KW - Female KW - Hepacivirus -- drug effects KW - Male KW - RNA, Viral -- blood KW - Antiviral Agents -- therapeutic use KW - Antiviral Agents -- administration & dosage KW - Hepatitis C, Chronic -- blood KW - Interferon-gamma -- therapeutic use KW - Hepatitis C, Chronic -- drug therapy KW - Hepatitis C, Chronic -- virology KW - Interferon-gamma -- administration & dosage KW - Antiviral Agents -- adverse effects KW - Interferon-gamma -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67927268?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+hepatology&rft.atitle=Pilot+study+of+interferon+gamma+for+chronic+hepatitis+C.&rft.au=Soza%2C+Alejandro%3BHeller%2C+Theo%3BGhany%2C+Marc%3BLutchman%2C+Glen%3BJake+Liang%2C+T%3BGermain%2C+June%3BHsu%2C+Henry+H%3BPark%2C+Yoon%3BHoofnagle%2C+Jay+H&rft.aulast=Soza&rft.aufirst=Alejandro&rft.date=2005-07-01&rft.volume=43&rft.issue=1&rft.spage=67&rft.isbn=&rft.btitle=&rft.title=Journal+of+hepatology&rft.issn=01688278&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-29 N1 - Date created - 2005-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Epidemic of mesothelioma in Egypt. AN - 67924571; 15950794 AB - Asbestos has been recognized in Egypt since a long time as ancient Egyptians were using it in mummification. Mesothelioma in Egypt is mainly attributed to environmental origin with a high incidence of women and young adults affected. The incidence of mesothelioma is rising in Egypt. Epidemiological data for 635 malignant mesothelioma (MM) patients over 4 years in the third Millennium were collected from the National Cancer Institute (NCI), Cairo University and Abbassia Chest hospital. This number is more than four times the number diagnosed in the previous 11 years at NCI. A clinicopathological study was done for 100 malignant pleural mesothelioma (MPM) patients and showed that asbestos exposure and SV40 positivity were evident in 67% and 60% of cases, respectively. The median survival was 14.3 months and the 1 and 2 year survival rates were 60% and 27%, respectively. Evaluation of p53 and pRb immunohistochemically showed that pRb alteration was related to poor survival. Other biological prognostic factors such as EGFR, HER-2, glutathione S transferase (GST) and MDR were evaluated in 50 cases. Overexpression of EGFR was correlated with lack of clinical benefit and poor survival. GST potentiated the effect of EGFR on survival. The use of EGFR inhibitors may have a role in the treatment of MM. Asbestos in Cairo is a silent killer and measures toward eliminating it entirely or at least strictly controlling human contact with this dangerous carcinogen have to be taken in order to combat the coming epidemic of mesothelioma in Egypt. JF - Lung cancer (Amsterdam, Netherlands) AU - Gaafar, R M AU - Eldin, N H Aly AD - Department of Medical Oncology, NCI, Cairo University, Cairo, Egypt. rababgaafar@link.net Y1 - 2005/07// PY - 2005 DA - July 2005 SP - S17 EP - S20 VL - 49 Suppl 1 SN - 0169-5002, 0169-5002 KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Humans KW - Egypt -- epidemiology KW - Adult KW - Male KW - Female KW - Mesothelioma -- epidemiology KW - Pleural Neoplasms -- epidemiology KW - Pleural Neoplasms -- pathology KW - Mesothelioma -- genetics KW - Pleural Neoplasms -- genetics KW - Mesothelioma -- pathology KW - Asbestos -- adverse effects KW - Disease Outbreaks UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67924571?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.atitle=Epidemic+of+mesothelioma+in+Egypt.&rft.au=Gaafar%2C+R+M%3BEldin%2C+N+H+Aly&rft.aulast=Gaafar&rft.aufirst=R&rft.date=2005-07-01&rft.volume=49+Suppl+1&rft.issue=&rft.spage=S17&rft.isbn=&rft.btitle=&rft.title=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.issn=01695002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-06 N1 - Date created - 2005-06-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Organochlorines in carpet dust and non-Hodgkin lymphoma. AN - 67922653; 15951670 AB - The incidence of non-Hodgkin lymphoma (NHL) has risen over the past several decades. Reasons for this increase are largely unexplained. In this population-based case-control study, we examined NHL risk and exposure to organochlorine compounds using concentrations in carpet dust as an exposure indicator. We identified NHL cases, uninfected with HIV, diagnosed between 1998 and 2000 among women and men ages 20-74 years in Iowa, Los Angeles County, and the Detroit and Seattle metropolitan areas. Controls were selected using random-digit-dialing or Medicare files. Organochlorine concentrations were measured in vacuum bag dust from 603 white cases and 443 white controls who had owned most of their carpets for at least 5 years. NHL risk was elevated if any of the polychlorinated biphenyl (PCB) congeners (PCBs 105, 138, 153, 170, or 180) was detected (odds ratio = 1.5; 95% confidence interval = 1.2-2.0). Risk was elevated in the top tertile of PCB 180 (1.7; 1.1-2.6) and in the top 2 tertiles of total PCBs (middle tertile, 1.6 [1.1-2.4]; top tertile 1.5 [1.0-2.2]). There was a positive trend in risk with increasing PCB 180 levels (P trend = 0.03). NHL risk was elevated if dichlorodiphenyldichloroethylene (DDE) was detected (1.3; 1.0-1.7), but only among men. A positive, but not monotonic, dose-response relationship was observed for DDE (P trend = 0.02). Our findings suggest an increased risk of NHL associated with exposure to PCBs, with evidence of greater effects for PCB 180. There is also some evidence of an association with DDE. JF - Epidemiology (Cambridge, Mass.) AU - Colt, Joanne S AU - Severson, Richard K AU - Lubin, Jay AU - Rothman, Nat AU - Camann, David AU - Davis, Scott AU - Cerhan, James R AU - Cozen, Wendy AU - Hartge, Patricia AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20852, USA. coltj@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 516 EP - 525 VL - 16 IS - 4 SN - 1044-3983, 1044-3983 KW - Dust KW - 0 KW - Environmental Pollutants KW - Hydrocarbons, Chlorinated KW - Pesticides KW - Index Medicus KW - Regression Analysis KW - Humans KW - European Continental Ancestry Group KW - SEER Program KW - Adult KW - Case-Control Studies KW - Aged KW - Middle Aged KW - United States -- epidemiology KW - Male KW - Female KW - Pesticides -- toxicity KW - Lymphoma, Non-Hodgkin -- epidemiology KW - Floors and Floorcoverings KW - Hydrocarbons, Chlorinated -- toxicity KW - Environmental Pollutants -- toxicity KW - Dust -- analysis KW - Lymphoma, Non-Hodgkin -- chemically induced KW - Environmental Exposure -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67922653?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiology+%28Cambridge%2C+Mass.%29&rft.atitle=Organochlorines+in+carpet+dust+and+non-Hodgkin+lymphoma.&rft.au=Colt%2C+Joanne+S%3BSeverson%2C+Richard+K%3BLubin%2C+Jay%3BRothman%2C+Nat%3BCamann%2C+David%3BDavis%2C+Scott%3BCerhan%2C+James+R%3BCozen%2C+Wendy%3BHartge%2C+Patricia&rft.aulast=Colt&rft.aufirst=Joanne&rft.date=2005-07-01&rft.volume=16&rft.issue=4&rft.spage=516&rft.isbn=&rft.btitle=&rft.title=Epidemiology+%28Cambridge%2C+Mass.%29&rft.issn=10443983&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-07 N1 - Date created - 2005-06-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - XRCC1 and DNA polymerase beta interaction contributes to cellular alkylating-agent resistance and single-strand break repair. AN - 67919669; 15838887 AB - X-ray cross complementing 1 (XRCC1) protein has been suggested to bind to DNA single-strand breaks (SSBs) and organize protein interactions that facilitate efficient DNA repair. Using four site-specifically modified human XRCC1 mutant expression systems and functional complementation assays in Chinese hamster ovary (CHO) XRCC1-deficient EM9 cells, we evaluated the cellular contributions of XRCC1s proposed N-terminal domain (NTD) DNA binding and DNA polymerase beta (POLbeta) interaction activities. Results within demonstrate that the interaction with POLbeta is biologically important for alkylating agent resistance and SSB repair, whereas the proposed DNA binding function is not critical to these phenotypes. Our data favor a model where the interaction of XRCC1 with POLbeta contributes to efficient DNA repair in vivo, whereas its interactions with target DNA is biologically less relevant. JF - Journal of cellular biochemistry AU - Wong, Heng-Kuan AU - Wilson, David M AD - Laboratory of Molecular Gerontology, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA. Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 794 EP - 804 VL - 95 IS - 4 SN - 0730-2312, 0730-2312 KW - Alkylating Agents KW - 0 KW - DNA, Single-Stranded KW - DNA-Binding Proteins KW - X-ray repair cross complementing protein 1 KW - Methyl Methanesulfonate KW - AT5C31J09G KW - DNA Polymerase beta KW - EC 2.7.7.- KW - Index Medicus KW - Animals KW - Humans KW - Mutation -- genetics KW - Protein Binding KW - Cell Line KW - Methyl Methanesulfonate -- pharmacology KW - Cricetinae KW - DNA, Single-Stranded -- metabolism KW - Alkylating Agents -- pharmacology KW - DNA-Binding Proteins -- genetics KW - Drug Resistance KW - DNA Repair -- drug effects KW - DNA Polymerase beta -- metabolism KW - DNA-Binding Proteins -- metabolism KW - DNA Damage -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67919669?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cellular+biochemistry&rft.atitle=XRCC1+and+DNA+polymerase+beta+interaction+contributes+to+cellular+alkylating-agent+resistance+and+single-strand+break+repair.&rft.au=Wong%2C+Heng-Kuan%3BWilson%2C+David+M&rft.aulast=Wong&rft.aufirst=Heng-Kuan&rft.date=2005-07-01&rft.volume=95&rft.issue=4&rft.spage=794&rft.isbn=&rft.btitle=&rft.title=Journal+of+cellular+biochemistry&rft.issn=07302312&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-06-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hepatic gene expression changes throughout the day in the Fischer rat: implications for toxicogenomic experiments. AN - 67910876; 15814895 AB - There is increasing use of transcriptional profiling in hepatotoxicity studies in the rat. Understanding hepatic gene expression changes over time is critical, since tissue collection may occur throughout the day. Furthermore, when comparing results from different data sets, times of dosing and tissue collection may vary. Circadian effects on the mouse hepatic transcriptome have been well documented. However, limited reports exist for the rat. In one study approximately 7% of the hepatic genes showed a diurnal expression pattern in a comparison of rat liver samples collected during the day versus livers collected at night. The results of a second study comparing rat liver samples collected at multiple time points over a circadian day suggest only minimal variation of the hepatic transcriptome. We studied temporal hepatic gene expression in 48 untreated F344/N rats using both approaches employed in these previous studies. Statistical analysis of microarray (SAM) identified differential expression in day/night comparisons, but was less sensitive for liver samples collected at multiple times of day. However, a Fourier analysis identified numerous periodically expressed genes in these samples including period genes, clock genes, clock-controlled genes, and genes involved in metabolic pathways. Furthermore, rhythms in gene expression were identified for several circadian genes not previously reported in the rat liver. Transcript levels for twenty genes involved in circadian and metabolic pathways were confirmed using quantitative RT-PCR. The results of this study demonstrate a prominent circadian rhythm in gene expression in the rat that is a critical factor in planning toxicogenomic experiments. JF - Toxicological sciences : an official journal of the Society of Toxicology AU - Boorman, Gary A AU - Blackshear, Pamela E AU - Parker, Joel S AU - Lobenhofer, Edward K AU - Malarkey, David E AU - Vallant, Molly K AU - Gerken, Diane K AU - Irwin, Richard D AD - Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. boorman@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 185 EP - 193 VL - 86 IS - 1 SN - 1096-6080, 1096-6080 KW - RNA, Messenger KW - 0 KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Oligonucleotide Array Sequence Analysis KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Messenger -- genetics KW - Male KW - Gene Expression Profiling KW - Liver -- metabolism KW - Toxicology KW - Pharmacogenetics KW - Circadian Rhythm -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67910876?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.atitle=Hepatic+gene+expression+changes+throughout+the+day+in+the+Fischer+rat%3A+implications+for+toxicogenomic+experiments.&rft.au=Boorman%2C+Gary+A%3BBlackshear%2C+Pamela+E%3BParker%2C+Joel+S%3BLobenhofer%2C+Edward+K%3BMalarkey%2C+David+E%3BVallant%2C+Molly+K%3BGerken%2C+Diane+K%3BIrwin%2C+Richard+D&rft.aulast=Boorman&rft.aufirst=Gary&rft.date=2005-07-01&rft.volume=86&rft.issue=1&rft.spage=185&rft.isbn=&rft.btitle=&rft.title=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Limited protective role of V-PYRRO/NO against cholestasis produced by alpha-naphthylisothiocyanate in mice. AN - 67906404; 15913567 AB - O(2)-vinyl 1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate (V-PYRRO/NO) is a liver-selective nitric oxide donor that has been shown to protect against hepatotoxic effects of endotoxin, acetaminophen and cadmium. This study examined the effects of V-PYRRO/NO on alpha-naphthylisothiocyanate (ANIT)-induced hepatotoxicity in mice. Mice were given V-PYRRO/NO via osmotic pumps (5.4mg/ml; 0.5 microl/h) starting 24h before receiving a hepatotoxic dose of ANIT (150mg/kg in olive oil, i.g.), and continuing for additional 48h (3-day pumps). V-PYRRO/NO administration partially ameliorated ANIT-induced hepatotoxicity, as evidenced by reduced serum alanine aminotransferase and alkaline phosphatase, markers of liver cell death, and by improved liver pathology. However, V-PYRRO/NO had no effect on ANIT-induced cholestasis, as ANIT-increased serum bilirubin levels and gamma-glutamyl transpeptidase activity were not ameliorated. Microarray and real time RT-PCR analysis revealed that ANIT intoxication altered expression of various genes, including genes encoding metabolic enzymes, transporter proteins, acute phase proteins, inflammation- and, apoptosis-related genes, as well as other genes related to liver injury. V-PYRRO/NO treatment attenuated ANIT-induced elevations in certain inflammation- and apoptosis-related genes, but had no effect on ANIT-induced disturbance on the expression of genes related to metabolism, transport, and acute phase proteins. Thus, the liver-selective NO donor, V-PYRRO/NO, was partially protective against ANIT-induced liver injury, without affecting ANIT-induced cholestasis and cholestasis-related gene expression. JF - Biochemical pharmacology AU - Liu, Jie AU - He, Yu-Ying AU - Chignell, Colin F AU - Clark, James AU - Myers, Page AU - Saavedra, Joseph E AU - Waalkes, Michael P AD - Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, NCI at NIEHS, Mail Drop F0-09, 111 Alexander Drive, Research Triangle Park, NC 27709, USA. liu6@niehs.nih.gov Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 144 EP - 151 VL - 70 IS - 1 SN - 0006-2952, 0006-2952 KW - Nitric Oxide Donors KW - 0 KW - O(2)-vinyl-1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate KW - Pyrrolidines KW - 1-Naphthylisothiocyanate KW - 551-06-4 KW - Index Medicus KW - Gene Expression Profiling KW - Animals KW - Liver -- drug effects KW - Liver -- metabolism KW - Mice KW - Male KW - Nitric Oxide Donors -- pharmacology KW - Cholestasis, Intrahepatic -- prevention & control KW - Pyrrolidines -- pharmacology KW - 1-Naphthylisothiocyanate -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67906404?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+pharmacology&rft.atitle=Limited+protective+role+of+V-PYRRO%2FNO+against+cholestasis+produced+by+alpha-naphthylisothiocyanate+in+mice.&rft.au=Liu%2C+Jie%3BHe%2C+Yu-Ying%3BChignell%2C+Colin+F%3BClark%2C+James%3BMyers%2C+Page%3BSaavedra%2C+Joseph+E%3BWaalkes%2C+Michael+P&rft.aulast=Liu&rft.aufirst=Jie&rft.date=2005-07-01&rft.volume=70&rft.issue=1&rft.spage=144&rft.isbn=&rft.btitle=&rft.title=Biochemical+pharmacology&rft.issn=00062952&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-15 N1 - Date created - 2005-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloid protein for solid-state NMR. AN - 67906258; 15939307 AB - Fibrillar protein aggregates contribute to the pathology of a number of disease states. To facilitate structural studies of these amyloid fibrils by solid-state NMR, efficient methods for the production of milligram quantities of isotopically labeled peptide are necessary. Bacterial expression of recombinant amyloid proteins and peptides allows uniform isotopic labeling, as well as other patterns of isotope incorporation. However, large-scale production of recombinant amyloidogenic peptides has proven particularly difficult, due to their inherent propensity for aggregation and the associated toxicity of fibrillar material. Yields of recombinant protein are further reduced by the small molecular weights of short amyloidogenic fragments. Here, we report high-yield expression and purification of a peptide comprising residues 11-26 of the Alzheimer's beta-amyloid protein (Abeta(11-26)), with homoserine lactone replacing serine at residue 26. Expression in inclusion bodies as a ketosteroid isomerase fusion protein and subsequent purification under denaturing conditions allows production of milligram quantities of uniformly labeled (13)C- and (15)N-labeled peptide, which forms amyloid fibrils suitable for solid-state NMR spectroscopy. Initial structural data obtained by atomic force microscopy, electron microscopy, and solid-state NMR measurements of Abeta(11-26) fibrils are also presented. JF - Protein expression and purification AU - Sharpe, Simon AU - Yau, Wai-Ming AU - Tycko, Robert AD - Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA. SimonS@intra.niddk.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 200 EP - 210 VL - 42 IS - 1 SN - 1046-5928, 1046-5928 KW - Amyloid KW - 0 KW - Amyloid beta-Peptides KW - Peptide Fragments KW - Recombinant Fusion Proteins KW - polyhistidine KW - 26062-48-6 KW - Histidine KW - 4QD397987E KW - Steroid Isomerases KW - EC 5.3.3.- KW - Cyanogen Bromide KW - OS382OHJ8P KW - Index Medicus KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Cyanogen Bromide -- chemistry KW - Escherichia coli -- genetics KW - Amino Acid Sequence KW - Chromatography, High Pressure Liquid KW - Cloning, Molecular KW - Amyloid -- biosynthesis KW - Base Sequence KW - Steroid Isomerases -- genetics KW - Gene Expression -- genetics KW - Histidine -- genetics KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization KW - Molecular Sequence Data KW - Amyloid -- ultrastructure KW - Recombinant Fusion Proteins -- metabolism KW - Recombinant Fusion Proteins -- biosynthesis KW - Amyloid beta-Peptides -- genetics KW - Peptide Fragments -- genetics KW - Nuclear Magnetic Resonance, Biomolecular -- methods KW - Amyloid beta-Peptides -- chemistry KW - Recombinant Fusion Proteins -- isolation & purification KW - Peptide Fragments -- isolation & purification KW - Amyloid beta-Peptides -- isolation & purification UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67906258?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+expression+and+purification&rft.atitle=Expression+and+purification+of+a+recombinant+peptide+from+the+Alzheimer%27s+beta-amyloid+protein+for+solid-state+NMR.&rft.au=Sharpe%2C+Simon%3BYau%2C+Wai-Ming%3BTycko%2C+Robert&rft.aulast=Sharpe&rft.aufirst=Simon&rft.date=2005-07-01&rft.volume=42&rft.issue=1&rft.spage=200&rft.isbn=&rft.btitle=&rft.title=Protein+expression+and+purification&rft.issn=10465928&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-05 N1 - Date created - 2005-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Health-related disparities: influence of environmental factors. AN - 67885396; 15925646 AB - Racial disparities in health cannot be explained solely on the basis of poverty, access to health care, behavior, or environmental factors. Their complex etiology is dependent on interactions between all these factors plus genetics. Scientists have been slow to consider genetics as a risk factor because genetic polymorphisms tend to be more variable within a race than between races. Now that studies are demonstrating the existence of racial differences in allelic frequencies for multiple genes affecting a single biologic mechanism, the present argument for a significant genetic role in contributing to health disparities is gaining support. Individuals vary, often significantly, in their response to environmental agents. This variability provides a high "background noise" when scientists examine human populations to identify environmental links to disease. This variability often masks important environmental contributors to disease risk and is a major impediment to efforts to investigate the causes of diseases.Fortunately, investments in the various genome projects have led to the development of tools and databases that can be used to help identify the genetic variations in environmental response genes that can lead to such wide differences in disease susceptibility. NIEHS developed the environ-mental genome project to catalog these genetic variants (polymorphisms)and to identify the ones that play a major role in human susceptibility to environmental agents. This information is being used in epidemiologic studies to pinpoint environmental contributors to disease better. The research summarized in this article is critically important for tying genetics and the environment to health disparities, and for the development of a rational approach to gauge environmental threats. Common variants in genes play pivotal roles in determining if or when illness or death result from exposure to drugs or environmental xenobiotics. Most common variants exist in all human populations, but their frequency can vary substantially,rendering individuals or groups more or less susceptible to particular environmental exposures. Such findings are consistent with the highly publicized analogy, "genetics loads the gun, but the environment pulls the trigger." That is, one can inherit the genetic predisposition to develop a disease but will do so only if or when exposed to the environmental trigger. Poor people have approximately the same genetic makeup as everyone else,but they have the unfortunate experience of living and working in environments containing multiple and high levels of carcinogens or other toxicants capable of interacting with susceptibility genes to cause disease.Furthermore, certain disadvantaged ethnic groups may have a higher incidence of certain susceptible genes that render them more vulnerable to adverse effects of the environments they inhabit. For both of these reasons,much of the nation's disease burden could likely be reduced through better environmental protection practices, especially in low-income and minority communities. Of the many implications of polymorphisms and frequency variations for public health and the practice of medicine, however, none is more urgent than the choice of drugs in therapy. Using such knowledge,randomized trials have identified race-specific drug response differences between blacks and whites [42].To date, most knowledge of the health effects of environmental factors is derived from studies of single agents. The reality, though, is that environmental contributions to health disparities are mostly from multiple agents. These simultaneous exposures to multiple risk factors, which may accumulate or interact synergistically, remain to be fully explained and defined.Finally, health disparity is a significant public health problem that cannot be solved using "business as usual" approaches for funding and priority setting. The current emphasis on basic and clinical research at the exclusion of public health and the social sciences does not provide the interdisciplinary research teams necessary to address such a complex problem as health disparities. Although the poor will always be with us, their health could be greatly improved if social, environmental, and genetic scientists could find ways to collaborate and develop more insightful and relevant ways to address the health of disadvantaged communities. JF - The Medical clinics of North America AU - Olden, Kenneth AU - White, Sandra L AD - National Institute of Environmental Health Sciences, United States Department of Health and Human Services, National Institutes of Health, PO Box 12233, Research Triangle Park, NC 27709, USA. olden@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 721 EP - 738 VL - 89 IS - 4 SN - 0025-7125, 0025-7125 KW - Abridged Index Medicus KW - Index Medicus KW - Environment KW - Gene Frequency KW - Disease Susceptibility -- epidemiology KW - Risk Factors KW - Humans KW - Genetic Predisposition to Disease -- epidemiology KW - Research KW - Obesity -- epidemiology KW - United States -- epidemiology KW - Delivery of Health Care -- statistics & numerical data KW - Environmental Exposure -- statistics & numerical data KW - Minority Groups -- statistics & numerical data UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67885396?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Medical+clinics+of+North+America&rft.atitle=Health-related+disparities%3A+influence+of+environmental+factors.&rft.au=Olden%2C+Kenneth%3BWhite%2C+Sandra+L&rft.aulast=Olden&rft.aufirst=Kenneth&rft.date=2005-07-01&rft.volume=89&rft.issue=4&rft.spage=721&rft.isbn=&rft.btitle=&rft.title=The+Medical+clinics+of+North+America&rft.issn=00257125&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-15 N1 - Date created - 2005-05-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic deletion of the norepinephrine transporter decreases vulnerability to seizures. AN - 67864545; 15911120 AB - Norepinephrine (NE) has been reported to modulate neuronal excitability and act as endogenous anticonvulsant. In the present study we used NE transporter knock-out mice (NET-KO), which are characterized by high levels of extracellular NE, to investigate the role of endogenous NE in seizure susceptibility. Seizure thresholds for cocaine (i.p.), pentylenetetrazol (i.v.) and kainic acid (i.v.) were compared in NET-KO, heterozygous (NET-HT) and wild type (NET-WT) female mice. The dose-response curve for cocaine-induced convulsions was significantly shifted to the right in NET-KO mice, indicating higher seizure thresholds. The threshold doses of pentylenetetrazol that induced clonic and tonic seizures were also significantly higher in NET-KO when compared to NET-WT mice. Similarly, NET-KO mice displayed higher resistance to convulsions engendered by kainic acid. For all drugs tested, the response of NET-HT mice was always intermediate. These data provide further support for a role of endogenous NE in the control of seizure susceptibility. JF - Neuroscience letters AU - Kaminski, Rafal M AU - Shippenberg, Toni S AU - Witkin, Jeffrey M AU - Rocha, Beatriz A AD - Integrative Neuroscience Section, NIDA/IRP, Baltimore, MD, USA. kaminskr@mail.nih.gov PY - 2005 SP - 51 EP - 55 VL - 382 IS - 1-2 SN - 0304-3940, 0304-3940 KW - Convulsants KW - 0 KW - Norepinephrine Plasma Membrane Transport Proteins KW - Slc6a2 protein, mouse KW - Symporters KW - Cocaine KW - I5Y540LHVR KW - Kainic Acid KW - SIV03811UC KW - Pentylenetetrazole KW - WM5Z385K7T KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Convulsants -- pharmacology KW - Mice, Inbred C57BL KW - Mice KW - Female KW - Mice, Knockout KW - Seizures -- chemically induced KW - Seizures -- physiopathology KW - Symporters -- genetics KW - Seizures -- genetics KW - Gene Deletion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67864545?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+letters&rft.atitle=Genetic+deletion+of+the+norepinephrine+transporter+decreases+vulnerability+to+seizures.&rft.au=Kaminski%2C+Rafal+M%3BShippenberg%2C+Toni+S%3BWitkin%2C+Jeffrey+M%3BRocha%2C+Beatriz+A&rft.aulast=Kaminski&rft.aufirst=Rafal&rft.date=2005-07-01&rft.volume=382&rft.issue=1-2&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Neuroscience+letters&rft.issn=03043940&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-11 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Neurosci. 2000 Dec 15;20(24):9040-5 [11124980] Trends Neurosci. 2000 Nov;23(11):580-7 [11074268] J Pharmacol Exp Ther. 2001 Sep;298(3):1042-8 [11504801] Pol J Pharmacol. 2001 Jan-Feb;53(1):57-60 [11785913] Neurology. 2002 Apr 23;58(8 Suppl 5):S27-39 [11971130] Pharmacol Ther. 2002 Jun;94(3):213-33 [12113799] Epilepsy Res. 2002 Jun;50(1-2):203-19 [12151130] Anesthesiology. 2002 Sep;97(3):693-700 [12218537] Neurology. 2002 Sep 10;59(5 Suppl 2):S14-7 [12221151] Neurology. 2002 Sep 24;59(6 Suppl 4):S48-55 [12270969] Epilepsia. 2002 Nov;43(11):1324-9 [12423381] Psychopharmacology (Berl). 2004 Jul;174(2):211-9 [14985936] Neurosci Biobehav Rev. 2004 Sep;28(5):507-24 [15465138] Exp Neurol. 1974 Oct;45(1):118-33 [4153438] Exp Neurol. 1980 Aug;69(2):395-407 [7409053] Eur J Pharmacol. 1982 Jan 22;77(2-3):163-6 [6277661] Brain Res. 1982 Jun 10;241(2):393-7 [6286046] Eur J Pharmacol. 1985 Jul 17;113(2):263-9 [2995067] Proc Natl Acad Sci U S A. 1986 Jun;83(11):4067-70 [3012548] Arch Int Pharmacodyn Ther. 1986 Aug;282(2):209-18 [2876688] Epilepsy Res. 1987 May;1(3):165-72 [2848694] Prog Brain Res. 1988;78:79-86 [3073428] Neurology. 1994 Nov;44(11):2105-10 [7969967] Brain Res. 1995 Sep 25;693(1-2):217-24 [8653412] J Pharmacol Exp Ther. 1999 May;289(2):703-11 [10215643] J Neurosci. 1999 Dec 15;19(24):10985-92 [10594079] Nat Neurosci. 2000 May;3(5):465-71 [10769386] Crit Rev Neurobiol. 1999;13(4):317-56 [11028680] J Pharmacol Exp Ther. 2001 Sep;298(3):986-95 [11504794] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ookinete-induced midgut peroxidases detonate the time bomb in anopheline mosquitoes. AN - 67831692; 15894189 AB - Previous analysis of the temporal-spatial relationship between ookinete migration and the cellular localization of genes mediating midgut immune defense responses suggested that, in order to survive, parasites must complete invasion before toxic chemicals ("a bomb") are generated by the invaded cell. Recent studies indicate that ookinete invasion induces tyrosine nitration as a two-step reaction, in which NOS induction is followed by a localized increase in peroxidase activity. Peroxidases utilize nitrite and hydrogen peroxide as substrates, and detonate the time bomb by generating reactive nitrogen intermediates, such as nitrogen dioxide, which mediate nitration. There is evidence that peroxidases also mediate antimicrobial responses to bacteria, fungi and parasites in a broad range of biological systems including humans and plants. Defense reactions that generate toxic chemicals are also potentially harmful to the host mounting the response and often results in apoptosis. The two-step nitration pathway is probably an ancient response, as it has also been described in vertebrate leukocytes and probably evolved as a mechanism to circumscribe the toxic products generated during defense responses involving protein nitration. JF - Insect biochemistry and molecular biology AU - Kumar, Sanjeev AU - Barillas-Mury, Carolina AD - Laboratory of Malaria and Vector Research, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 721 EP - 727 VL - 35 IS - 7 SN - 0965-1748, 0965-1748 KW - Peroxidases KW - EC 1.11.1.- KW - Index Medicus KW - Animals KW - Enzyme Induction -- immunology KW - Apoptosis KW - Host-Parasite Interactions KW - Gastrointestinal Tract -- enzymology KW - Zygote -- immunology KW - Anopheles -- immunology KW - Anopheles -- enzymology KW - Plasmodium berghei -- immunology KW - Peroxidases -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67831692?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Insect+biochemistry+and+molecular+biology&rft.atitle=Ookinete-induced+midgut+peroxidases+detonate+the+time+bomb+in+anopheline+mosquitoes.&rft.au=Kumar%2C+Sanjeev%3BBarillas-Mury%2C+Carolina&rft.aulast=Kumar&rft.aufirst=Sanjeev&rft.date=2005-07-01&rft.volume=35&rft.issue=7&rft.spage=721&rft.isbn=&rft.btitle=&rft.title=Insect+biochemistry+and+molecular+biology&rft.issn=09651748&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-30 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Acute administration of SB-277011A, NGB 2904, or BP 897 inhibits cocaine cue-induced reinstatement of drug-seeking behavior in rats: role of dopamine D3 receptors. AN - 67788784; 15858839 AB - Recent studies have shown that the novel dopamine (DA) D3 receptor antagonists SB-277011A and NGB 2904 inhibit cocaine- and/or stress-induced reinstatement of drug-seeking behavior. The present study sought to determine if SB-277011A, NGB 2904, or BP-897 (a mixed D3 agonist/antagonist) similarly inhibit cocaine-associated cue-induced reinstatement of drug-seeking behavior. Long-Evans rats were allowed to self-administer cocaine. Each cocaine infusion was paired with discrete conditioned cue-light and tone. Subsequently, drug-seeking (i.e., lever-pressing) behavior was extinguished in the absence of cocaine and cocaine-associated cues. Rats were then tested for cue-induced reinstatement of drug-seeking. We found that cocaine-associated cues evoked robust reinstatement of lever-pressing. Acute intraperitoneal (i.p.) administration of SB-277011A (6, 12, or 24 mg/kg) produced a dose-dependent inhibition of cue-induced reinstatement of drug-seeking behavior by 35, 65, and 85%, respectively, compared to vehicle-treated animals. Acute i.p. administration of NGB 2904 (0.1, 1.0, or 5.0 mg/kg) produced a 45, 30, and 70% inhibition of cue-induced reinstatement, respectively, compared to vehicle-treated animals. Acute i.p. administration of either 0.1 or 1 mg/kg of BP 897 did not produce a significant effect on cue-induced reinstatement, whereas a dose of 3 mg/kg produced a 70% inhibition of cue-induced reinstatement. These findings, combined with previous data, suggest that DA D3 receptor antagonism may underlie the inhibitory effects of SB-277011A and NGB 2904 on cocaine cue-induced reinstatement, while the effects of BP 897 may involve D3 and non-D3 receptor mechanisms. JF - Synapse (New York, N.Y.) AU - Gilbert, Jeremy G AU - Newman, Amy Hauck AU - Gardner, Eliot L AU - Ashby, Charles R AU - Heidbreder, Christian A AU - Pak, Arlene C AU - Peng, Xiao-Qing AU - Xi, Zheng-Xiong AD - Neuropsychopharmacology Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, DHHS, Baltimore, Maryland 21224, USA. Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 17 EP - 28 VL - 57 IS - 1 SN - 0887-4476, 0887-4476 KW - BP 897 KW - 0 KW - Dopamine Antagonists KW - Dopamine D2 Receptor Antagonists KW - Drd3 protein, rat KW - Fluorenes KW - NGB 2904 KW - Nitriles KW - Piperazines KW - Receptors, Dopamine D3 KW - SB 277011 KW - Tetrahydroisoquinolines KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Rats KW - Conditioning, Operant -- drug effects KW - Animals KW - Photic Stimulation KW - Self Administration KW - Rats, Long-Evans KW - Extinction, Psychological -- drug effects KW - Recurrence KW - Male KW - Fluorenes -- pharmacology KW - Nitriles -- pharmacology KW - Dopamine Antagonists -- pharmacology KW - Cocaine-Related Disorders -- psychology KW - Cocaine-Related Disorders -- drug therapy KW - Cues KW - Tetrahydroisoquinolines -- pharmacology KW - Piperazines -- pharmacology KW - Cocaine -- pharmacology KW - Cocaine -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67788784?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Synapse+%28New+York%2C+N.Y.%29&rft.atitle=Acute+administration+of+SB-277011A%2C+NGB+2904%2C+or+BP+897+inhibits+cocaine+cue-induced+reinstatement+of+drug-seeking+behavior+in+rats%3A+role+of+dopamine+D3+receptors.&rft.au=Gilbert%2C+Jeremy+G%3BNewman%2C+Amy+Hauck%3BGardner%2C+Eliot+L%3BAshby%2C+Charles+R%3BHeidbreder%2C+Christian+A%3BPak%2C+Arlene+C%3BPeng%2C+Xiao-Qing%3BXi%2C+Zheng-Xiong&rft.aulast=Gilbert&rft.aufirst=Jeremy&rft.date=2005-07-01&rft.volume=57&rft.issue=1&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=Synapse+%28New+York%2C+N.Y.%29&rft.issn=08874476&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-12 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Med Chem. 2001 Sep 13;44(19):3175-86 [11543687] Xenobiotica. 2001 Aug-Sep;31(8-9):677-86 [11569533] J Neurosci. 2001 Dec 1;21(23):9471-7 [11717381] Pharmacol Biochem Behav. 2002 Mar;71(3):517-29 [11830186] Pharmacol Rev. 2002 Mar;54(1):1-42 [11870259] Neuroreport. 2002 Jan 21;13(1):173-6 [11924883] J Neurosci. 2002 May 1;22(9):3312-20 [11978805] Eur J Neurosci. 2002 Jun;15(12):2016-26 [12099907] Behav Pharmacol. 2002 Sep;13(5-6):397-405 [12394416] J Neurosci. 2002 Nov 1;22(21):9595-603 [12417684] Neuroreport. 2003 Jan 20;14(1):93-8 [12544838] Neuropsychopharmacology. 2003 Feb;28(2):329-38 [12589386] Synapse. 2003 Jun 1;48(3):154-6 [12645041] Neuropsychopharmacology. 2003 May;28(5):839-49 [12637956] Neuropsychopharmacology. 2003 Jun;28(6):1150-9 [12700684] Bioorg Med Chem Lett. 2003 Jul 7;13(13):2179-83 [12798330] Neuropsychopharmacology. 2003 Jul;28(7):1272-80 [12700694] CNS Drug Rev. 2003 Summer;9(2):141-58 [12847556] Brain Res Bull. 2003 Oct 15;61(6):595-601 [14519456] Behav Neurosci. 2003 Oct;117(5):952-60 [14570545] Neuropsychopharmacology. 2003 Nov;28(11):1903-15 [12915863] Neurosci Biobehav Rev. 2003 Oct;27(6):543-54 [14599435] Pharmacol Biochem Behav. 2004 Feb;77(2):309-18 [14751459] Eur J Neurosci. 2004 Mar;19(6):1661-7 [15066162] Eur J Neurosci. 2004 Jul;20(1):249-63 [15245497] Neuropsychopharmacology. 2004 Aug;29(8):1479-87 [15100700] J Neurosci. 2004 Aug 11;24(32):7167-73 [15306650] Psychopharmacology (Berl). 2004 Oct;176(1):57-65 [15083257] Eur J Pharmacol. 1988 Jul 7;151(2):233-42 [2844553] Nature. 1990 Sep 13;347(6289):146-51 [1975644] Int J Addict. 1990-1991;25(7A-8A):957-93 [2131326] Biochem Pharmacol. 1992 Feb 18;43(4):659-66 [1347215] Brain Res. 1991 Nov 15;564(2):203-19 [1839781] Psychopharmacology (Berl). 1992;107(4):523-9 [1603895] Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8155-9 [1518841] Brain Res. 1993 Nov 26;629(1):31-9 [8287278] NIDA Res Monogr. 1993;137:73-95 [8289929] Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11271-5 [7972046] J Neurosci. 1996 Oct 1;16(19):6100-6 [8815892] Psychopharmacology (Berl). 1996 Feb;123(3):280-8 [8833421] Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):12040-5 [8876259] Psychopharmacology (Berl). 1996 Oct;127(3):213-24 [8912399] Neuropsychopharmacology. 1996 Nov;15(5):506-14 [8914124] Brain Res Mol Brain Res. 1997 May;45(2):335-9 [9149110] Mol Psychiatry. 1997 Jan;2(1):5-6 [9154208] Pharmacol Rev. 1997 Sep;49(3):231-52 [9311022] Science. 1997 Oct 3;278(5335):66-70 [9311929] Neuron. 1997 Sep;19(3):591-611 [9331351] Behav Brain Res. 1997 Sep;87(2):139-48 [9331482] Psychopharmacology (Berl). 1998 Jan;135(2):151-60 [9497020] Eur J Neurosci. 1998 Mar;10(3):1113-20 [9753179] Bioorg Med Chem Lett. 1998 Oct 6;8(19):2715-8 [9873609] Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4321-6 [10760299] J Med Chem. 2000 May 4;43(9):1878-85 [10794704] J Pharmacol Exp Ther. 2000 Sep;294(3):1154-65 [10945872] Am J Psychiatry. 1999 Jan;156(1):11-8 [9892292] Nature. 1999 Jul 22;400(6742):371-5 [10432116] Psychopharmacologia. 1960 Feb 12;1:251-6 [13834123] Recent Adv Biol Psychiatry. 1961;4:288-309 [13916635] Psychopharmacology (Berl). 2004 Nov;176(3-4):459-65 [15138757] Neuropsychopharmacology. 2005 Feb;30(2):296-309 [15483559] Brain Res Brain Res Rev. 2005 Jul;49(1):77-105 [15960988] J Neurosci. 2000 Oct 1;20(19):7489-95 [11007908] Eur J Pharmacol. 2000 Oct 27;407(1-2):47-51 [11050289] Am J Psychiatry. 2000 Nov;157(11):1789-98 [11058476] J Neurosci. 2000 Dec 1;20(23):8677-84 [11102473] Behav Brain Res. 2001 May;120(2):147-58 [11182163] Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1976-81 [11172061] Behav Pharmacol. 2001 Feb;12(1):1-11 [11270507] Psychopharmacology (Berl). 2001 Mar;154(3):301-10 [11351937] Nature. 2001 Jul 12;412(6843):141-2 [11449260] Ann N Y Acad Sci. 2001 Jun;937:1-26 [11458532] Eur J Pharmacol. 2001 Jul 20;424(2):85-90 [11476753] Pharmacol Biochem Behav. 2001 Jul-Aug;69(3-4):555-60 [11509216] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Basic and Applied Decision Making in Cancer Control AN - 57140065; 200605417 AB - Decision making is fundamental to all aspects of cancer care -- prevention, detection, treatment, survivorship, & end of life -- yet researchers & clinicians have limited knowledge of the ways in which patients & their health care providers make critical health decisions. Recognizing how important it is to understand how patients & their providers make potentially life-altering decisions, the National Cancer Institute developed a decision making in cancer control initiative. The goal of this initiative is to enhance understanding of human decision-making processes so that individuals can make more informed & satisfying choices regarding their health. This article describes the multidisciplinary meeting that provided the scientific foundation for this initiative. 2 Appendixes, 47 References. [Copyright 2005 The American Psychological Association.] JF - Health Psychology AU - Nelson, Wendy AU - Peters, Ellen AU - Stefanek, Michael AU - McCaul, Kevin D AD - National Cancer Instit, Bethesda, MD nelsonw@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - S3 EP - S8 PB - American Psychological Association, Washington DC VL - 24 IS - 4 SN - 0278-6133, 0278-6133 KW - cancer, decision making, judgment and decision making KW - Clinical decision making KW - Patient participation KW - Patient control KW - Cancer KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57140065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+Psychology&rft.atitle=Basic+and+Applied+Decision+Making+in+Cancer+Control&rft.au=Nelson%2C+Wendy%3BPeters%2C+Ellen%3BStefanek%2C+Michael%3BMcCaul%2C+Kevin+D&rft.aulast=Nelson&rft.aufirst=Wendy&rft.date=2005-07-01&rft.volume=24&rft.issue=4&rft.spage=S3&rft.isbn=&rft.btitle=&rft.title=Health+Psychology&rft.issn=02786133&rft_id=info:doi/10.1037%2F0278-6133.24.4.S3 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-05-02 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Cancer; Clinical decision making; Patient participation; Patient control DO - http://dx.doi.org/10.1037/0278-6133.24.4.S3 ER - TY - JOUR T1 - Making Sense of Number Sense: Implications for Children with Mathematical Disabilities AN - 57107989; 200601053 AB - Drawing on various approaches to the study of mathematics learning, Gersten, Jordan, and Flojo (in this issue) explore the implications of this research for identifying children at risk for developing mathematical disabilities. One of the key topics Gersten et al. consider in their review is that of 'number sense.' I expand on their preliminary effort by examining in detail the diverse set of components purported to be encompassed by this construct. My analysis reveals some major differences between the ways in which number sense is defined in the mathematical cognition literature and its definition in the literature in mathematics education. I also present recent empirical evidence and theoretical perspectives bearing on the importance of measuring the speed of making magnitude comparisons. Finally, I discuss how differing conceptions of number sense inform the issue of whether and to what extent it may be teachable. Illustrations, References. Adapted from the source document. JF - Journal of Learning Disabilities AU - Berch, Daniel B AD - Child Development & Behavior Branch, National Instit Child Health & Human Development, Bethesda, MD berchd2@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 333 EP - 339 VL - 38 IS - 4 SN - 0022-2194, 0022-2194 KW - Perception KW - Numbers KW - Learning disabilities KW - Children KW - Mathematics KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57107989?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Learning+Disabilities&rft.atitle=Making+Sense+of+Number+Sense%3A+Implications+for+Children+with+Mathematical+Disabilities&rft.au=Berch%2C+Daniel+B&rft.aulast=Berch&rft.aufirst=Daniel&rft.date=2005-07-01&rft.volume=38&rft.issue=4&rft.spage=333&rft.isbn=&rft.btitle=&rft.title=Journal+of+Learning+Disabilities&rft.issn=00222194&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JLDIAD N1 - SubjectsTermNotLitGenreText - Mathematics; Children; Learning disabilities; Numbers; Perception ER - TY - JOUR T1 - Duration and Developmental Timing of Poverty and Children's Cognitive and Social Development from Birth through Third Grade AN - 57097091; 200600964 AB - Relations of duration and developmental timing of poverty to children's development from birth to age 9 were examined by comparing children from families who were never poor, poor only during the child's infancy (0-3 years of age), poor only after infancy (4-9 years of age), and chronically poor. Chronically poor families provided lower quality childrearing environments, and children in these families showed lower cognitive performance and more behavior problems than did other children. Any experience of poverty was associated with less favorable family situations and child outcomes than never being poor. Being poor later tended to be more detrimental than early poverty. Mediational analyses indicated that poverty was linked to child outcomes in part through less positive parenting. Illustrations, Tables, References. Adapted from the source document. JF - Child Development AU - -- -- -- AD - NICHD Early Child Care Research Network, OEP, Rockville, MD Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 795 EP - 810 VL - 76 IS - 4 SN - 0009-3920, 0009-3920 KW - Timing KW - Poverty KW - Child rearing KW - Cognitive development KW - Social development KW - Child development KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57097091?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Child+Development&rft.atitle=Duration+and+Developmental+Timing+of+Poverty+and+Children%27s+Cognitive+and+Social+Development+from+Birth+through+Third+Grade&rft.au=--+--+--&rft.aulast=--+--+--&rft.aufirst=&rft.date=2005-07-01&rft.volume=76&rft.issue=4&rft.spage=795&rft.isbn=&rft.btitle=&rft.title=Child+Development&rft.issn=00093920&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - CHDEAW N1 - SubjectsTermNotLitGenreText - Timing; Poverty; Child development; Child rearing; Social development; Cognitive development ER - TY - JOUR T1 - Combining Treatments for Alcoholism: Why and How? AN - 57081444; 200600544 AB - Treatment of alcohol disorders through the use of combinations of pharmacological and behavioral modalities may more effectively address the multicomponent nature of the disorder than single-modality approaches. Interdisciplinary models of the biological, psychological and social components of alcohol disorders are emerging rapidly from basic research, and treatment researchers have begun to test various strategies to combine medications and behavioral treatments. In addition to behavioral and pharmacological combinations, effective treatment pairs can be composed of two medications whose mechanisms of action are believed to be compatible and potentially additive, or even synergistic. Combining Medications and Behavioral Interventions (COMBINE) is a large multisite clinical trial sponsored by the National Institute on Alcohol Abuse and Alcoholism. Its goal is to determine if improvements in treatment outcome for alcohol dependence can be achieved by combining pharmacotherapy and behavioral interventions. Under evaluation is the efficacy of two promising medications (naltrexone and acamprosate), both singly and together, when used in conjunction with two behavioral treatments of differing intensities. This supplement describes in detail the methods and rationale for the approach taken in COMBINE. This first article in the supplement has three objectives: (1) to review strategies for conducting combination treatment studies as illustrated with selected examples from the literature, (2) to summarize the main design features of COMBINE as background for the articles in this supplement and (3) to comment on future directions for combination treatment research as the field moves beyond COMBINE. Illustrations, References. Adapted from the source document. JF - Journal of Studies on Alcohol AU - Mattson, Margaret E AU - Litten, Raye Z AD - Division Treatment & Recovery Research, National Instit Alcohol Abuse & Alcoholism, Bethesda, MD mmattson@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 8 EP - 16 SN - 0096-882X, 0096-882X KW - Interventions KW - Naltrexone KW - Alcoholism KW - Clinical trials KW - Treatment KW - Acamprosate KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57081444?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Studies+on+Alcohol&rft.atitle=Combining+Treatments+for+Alcoholism%3A+Why+and+How%3F&rft.au=Mattson%2C+Margaret+E%3BLitten%2C+Raye+Z&rft.aulast=Mattson&rft.aufirst=Margaret&rft.date=2005-07-01&rft.volume=&rft.issue=&rft.spage=8&rft.isbn=&rft.btitle=&rft.title=Journal+of+Studies+on+Alcohol&rft.issn=0096882X&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JSALDP N1 - SubjectsTermNotLitGenreText - Alcoholism; Treatment; Interventions; Naltrexone; Acamprosate; Clinical trials ER - TY - JOUR T1 - COMBINE Genetics Study: The Pharmacogenetics of Alcoholism Treatment Response: Genes and Mechanisms AN - 57075155; 200600306 AB - Objective: Partial efficacy of treatment and differences in adverse events across individuals are a challenge and an opportunity in the treatment of alcoholism. Individuation of therapy and understanding origins of differential treatment response may require identification of inherited functional variants of genes. The neurobiology of reward, executive cognitive function, anxiety and dysphoria have been identified as critical domains that may have a genetic basis that could predict treatment response. Method: The COMBINE Study presents a unique opportunity to evaluate specific genetic loci (markers) that affect neurobiology central to addiction and extended withdrawal. The study also addresses variation in drug metabolism and action. Candidate genetic markers are selected for study based on functionality and abundance. Results: COMT Val158Met is a common (minor allele frequency 0.42), functional, catecholamine-metabolizing enzyme polymorphism with threefold relevance. Val 158Met alters executive cognitive function, stress and anxiety responses and brain endogenous opioid function. OPRM1 Asn40Asp is a common (minor allele frequency 0.10), functional polymorphism of the mu-opioid receptor, which may serve as a gatekeeper molecule in naltrexone's actions and was recently reported to affect naltrexone response. HTTLPR (minor allele frequency 0.40) alters serotonin transporter function to affect anxiety, dysphoria and obsessional behavior, which are assessed in COMBINE and may be related to relapse and addictive behavior. Conclusions: All genetic testing is consented through a separate human research protocol, and the testing is conducted nonclinically, confidentially and apart from the clinical record to protect human research participants who have volunteered for this aspect of COMBINE. Illustrations, Tables, References. Adapted from the source document. JF - Journal of Studies on Alcohol AU - Goldman, David AU - Oroszi, Gabor AU - O'Malley, Stephanie AU - Anton, Raymond AD - Laboratory of Neurogenetics, NIAAA, NIH, Rockville, MD Y1 - 2005/07// PY - 2005 DA - July 2005 SP - 56 EP - 64 SN - 0096-882X, 0096-882X KW - Genetics KW - Alcoholism KW - Naltrexone KW - Treatment KW - Clinical trials KW - Pharmacogenetics KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57075155?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Studies+on+Alcohol&rft.atitle=COMBINE+Genetics+Study%3A+The+Pharmacogenetics+of+Alcoholism+Treatment+Response%3A+Genes+and+Mechanisms&rft.au=Goldman%2C+David%3BOroszi%2C+Gabor%3BO%27Malley%2C+Stephanie%3BAnton%2C+Raymond&rft.aulast=Goldman&rft.aufirst=David&rft.date=2005-07-01&rft.volume=&rft.issue=&rft.spage=56&rft.isbn=&rft.btitle=&rft.title=Journal+of+Studies+on+Alcohol&rft.issn=0096882X&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2006-04-07 N1 - Last updated - 2016-09-27 N1 - CODEN - JSALDP N1 - SubjectsTermNotLitGenreText - Pharmacogenetics; Naltrexone; Clinical trials; Alcoholism; Treatment; Genetics ER - TY - JOUR T1 - Birth rights and rituals in rural south India: care seeking in the intrapartum period AN - 38207549; 2981824 AB - Maternal morbidity and mortality are high in the Indian context, but the majority of maternal deaths could be avoided by prompt and effective access to intrapartum care (WHO, 1999). Understanding the care seeking responses to intrapartum morbidities is crucial if maternal health is to be effectively improved, and maternal mortality reduced. This paper presents the results of a prospective study of 388 women followed through delivery and traditional postpartum in rural Karnataka in southern India. In this setting, few women use the existing health facilities and most deliveries occur at home. The analysis uses quantitative data, collected via questionnaires administered to women both during pregnancy and immediately after delivery. By virtue of its prospective design, the study gives a unique insight into intentions for intrapartum care during pregnancy as well as events following morbidities during labour. Routine care in the intrapartum period, both within institutions and at home, and impediments to appropriate care are also examined. The study was designed to collect information about health seeking decisions made by women and their families as pregnancies unfolded, rather than trying to capture women's experience from a retrospective instrument. The data set is therefore a rich source of quantitative information, which incorporates details of event sequences and health service utilization not previously collected in a Safe Motherhood study. Additional qualitative information was also available from concurrent in-depth interviews with pregnant women, their families, health care providers and other key informants in the area. The level of unplanned institutional care seeking during the intrapartum period within the study area was very high, increasing from 11% planning deliveries at a facility to an eventual 35% actually delivering in hospitals. In addition there was a significant move away from planned deliveries with the auxiliary nurse midwive (ANM), to births with a lay attendant or dai. The proportion of women who planned for an ANM to assist was 49%, as compared with the actual occurrence, which was less than half of this proportion. Perceived quality of care was found to be an important factor in health seeking behaviour, as was wealth, caste, education and experience of previous problems in pregnancy. Actual care given by a range of practitioners was found to contain both beneficial and undesirable elements. As a response to serious morbidities experienced within the study period, many women were able to seek care although sometimes after a long delay. Those women who experienced inadequate progression of labour pains were most likely to proceed unexpectedly to a hospital delivery. Reprinted by permission of Cambridge University Press. An electronic version of this article can be accessed via the internet at http://journals.cambridge.org JF - Journal of biosocial science AU - Matthews, Zoë AU - Ramakrishna, Jayashree AU - Mahendra, Shanti AU - Kilaru, Asha AU - Ganapathy, Saraswathy AD - University of Southampton ; National Institute of Mental Health and Neuro Sciences ; University of North Carolina Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 385 EP - 412 VL - 37 IS - 4 SN - 0021-9320, 0021-9320 KW - Anthropology KW - Sociology KW - Birth KW - Mortality KW - Rights KW - Women's health KW - Reproductive health KW - Social sciences KW - Science and technology KW - Morbidity KW - India KW - Rural areas UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38207549?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+biosocial+science&rft.atitle=Birth+rights+and+rituals+in+rural+south+India%3A+care+seeking+in+the+intrapartum+period&rft.au=Matthews%2C+Zo%C3%AB%3BRamakrishna%2C+Jayashree%3BMahendra%2C+Shanti%3BKilaru%2C+Asha%3BGanapathy%2C+Saraswathy&rft.aulast=Matthews&rft.aufirst=Zo%C3%AB&rft.date=2005-07-01&rft.volume=37&rft.issue=4&rft.spage=385&rft.isbn=&rft.btitle=&rft.title=Journal+of+biosocial+science&rft.issn=00219320&rft_id=info:doi/10.1017%2FS0021932004006911 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 11326 11325 12622; 11920; 1635 11574; 11032 9705; 11156 1247; 10893 5772; 8291 3409 6306; 13609 5772; 8285 3409 6306; 175 387 30 DO - http://dx.doi.org/10.1017/S0021932004006911 ER - TY - JOUR T1 - Gastrointestinal Stromal Tumor (GIST) of the Esophagus Detected by Positron Emission Tomography/Computed Tomography AN - 21280779; 6788889 JF - Digestive Diseases and Sciences AU - Chang, Wei-chou AU - Tzao, Ching AU - Hueng-yuan Shen, Daniel AU - Cheng, Cheng-yi AU - Yu, Cheng-ping AU - Hsu, Hsian-he AD - Tri-Service General Hospital, 325, Sec. 2, Cheng Gong Road, Nei Hu, Taipei 114, Taiwan, Republic of China Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1315 EP - 1318 VL - 50 IS - 7 SN - 0163-2116, 0163-2116 KW - Biotechnology and Bioengineering Abstracts KW - Esophagus KW - Computed tomography KW - Positron emission tomography KW - Tumors KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21280779?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Digestive+Diseases+and+Sciences&rft.atitle=Gastrointestinal+Stromal+Tumor+%28GIST%29+of+the+Esophagus+Detected+by+Positron+Emission+Tomography%2FComputed+Tomography&rft.au=Chang%2C+Wei-chou%3BTzao%2C+Ching%3BHueng-yuan+Shen%2C+Daniel%3BCheng%2C+Cheng-yi%3BYu%2C+Cheng-ping%3BHsu%2C+Hsian-he&rft.aulast=Chang&rft.aufirst=Wei-chou&rft.date=2005-07-01&rft.volume=50&rft.issue=7&rft.spage=1315&rft.isbn=&rft.btitle=&rft.title=Digestive+Diseases+and+Sciences&rft.issn=01632116&rft_id=info:doi/10.1007%2Fs10620-005-2779-2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-03-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Esophagus; Computed tomography; Positron emission tomography; Tumors DO - http://dx.doi.org/10.1007/s10620-005-2779-2 ER - TY - JOUR T1 - Pesticide Testing on Human Subjects: Weighing Benefits and Risks AN - 20726260; 6890383 AB - In the debate surrounding testing pesticides on human subjects, two distinct positions have emerged. The first position holds that pesticide experiments on human subjects should be allowed, but only under stringent scientific and ethical standards. The second position asserts that these experiments should never be allowed. In this article, we evaluate what we consider to be the strongest argument for the second position - namely, that the benefits of the experiments are not significant enough to justify the risks posed to healthy subjects. We challenge this argument by examining the benefits and risks of testing pesticides on human subjects. We argue that a study that intentionally exposes humans subjects to pesticides should be permitted if a) the knowledge gained from the study is expected to promote human health; b) the knowledge cannot be reasonably obtained by other means; c) the study is not expected to cause serious or irreversible harm to the subjects; and d) appropriate safeguards are in place to minimize harm to the subjects. JF - Environmental Health Perspectives AU - Resnik, D B AU - Portier, C AD - NIEHS/NIH, P.O. Box 12233, Mail Drop NH-06, Research Triangle Park, NC 27709, USA, resnikd@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 813 EP - 817 VL - 113 IS - 7 SN - 0091-6765, 0091-6765 KW - Risk Abstracts; Toxicology Abstracts KW - Ethics KW - Pesticides KW - Toxicity testing KW - X 24222:Analytical procedures KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20726260?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Pesticide+Testing+on+Human+Subjects%3A+Weighing+Benefits+and+Risks&rft.au=Resnik%2C+D+B%3BPortier%2C+C&rft.aulast=Resnik&rft.aufirst=D&rft.date=2005-07-01&rft.volume=113&rft.issue=7&rft.spage=813&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7720 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Ethics; Pesticides; Toxicity testing DO - http://dx.doi.org/10.1289/ehp.7720 ER - TY - JOUR T1 - Lipid Adjustment in the Analysis of Environmental Contaminants and Human Health Risks AN - 20721272; 6890388 AB - The literature on exposure to lipophilic agents such as polychlorinated biphenyls (PCBs) is conflicting, posing challenges for the interpretation of potential human health risks. Laboratory variation in quantifying PCBs may account for some of the conflicting study results. For example, for quantification purposes, blood is often used as a proxy for adipose tissue, which makes it necessary to model serum lipids when assessing health risks of PCBs. Using a simulation study, we evaluated four statistical models (unadjusted, standardized, adjusted, and two-stage) for the analysis of PCB exposure, serum lipids, and health outcome risk (breast cancer). We applied eight candidate true causal scenarios, depicted by directed acyclic graphs, to illustrate the ramifications of misspecification of underlying assumptions when interpreting results. Statistical models that deviated from underlying causal assumptions generated biased results. Lipid standardization, or the division of serum concentrations by serum lipids, was observed to be highly prone to bias. We conclude that investigators must consider biology, biologic medium (e.g., nonfasting blood samples), laboratory measurement, and other underlying modeling assumptions when devising a statistical plan for assessing health outcomes in relation to environmental exposures. JF - Environmental Health Perspectives AU - Schisterman, E F AU - Whitcomb, B W AU - Louis, GMB AU - Louis, T A AD - Epidemiology Branch, Division of Epidemiology, Statistics and Prevention Research, National Institute of Child Health and Human Development, 6100 Executive Blvd., Room 7B03, Rockville, MD 20852, USA, schistee@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 853 EP - 857 VL - 113 IS - 7 SN - 0091-6765, 0091-6765 KW - Pollution Abstracts; Risk Abstracts; Toxicology Abstracts KW - Risk assessment KW - Mathematical models KW - Serum lipids KW - Lipids KW - adipose tissues KW - Statistical analysis KW - Simulation KW - Environmental factors KW - Cancer KW - Lipophilic KW - Standardization KW - polychlorinated biphenyls KW - Breast cancer KW - Adipose tissue KW - Standards KW - Contaminants KW - PCB compounds KW - PCB KW - X 24222:Analytical procedures KW - R2 23060:Medical and environmental health KW - P 6000:TOXICOLOGY AND HEALTH UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20721272?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Lipid+Adjustment+in+the+Analysis+of+Environmental+Contaminants+and+Human+Health+Risks&rft.au=Schisterman%2C+E+F%3BWhitcomb%2C+B+W%3BLouis%2C+GMB%3BLouis%2C+T+A&rft.aulast=Schisterman&rft.aufirst=E&rft.date=2005-07-01&rft.volume=113&rft.issue=7&rft.spage=853&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7640 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-07-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Risk assessment; Standardization; Mathematical models; polychlorinated biphenyls; Lipids; Serum lipids; Statistical analysis; Adipose tissue; Breast cancer; Environmental factors; Lipophilic; PCB; adipose tissues; Simulation; Standards; Contaminants; PCB compounds; Cancer DO - http://dx.doi.org/10.1289/ehp.7640 ER - TY - JOUR T1 - The Elevated 10-Year Risk of Cervical Precancer and Cancer in Women With Human Papillomavirus (HPV) Type 16 or 18 and the Possible Utility of Type-Specific HPV Testing in Clinical Practice AN - 20719259; 6477437 AB - BACKGROUND: Human papillomavirus (HPV) types 16 and 18 cause 60%-70% of cervical cancer worldwide, and other HPV types cause virtually all remaining cases. Pooled HPV testing for 13 oncogenic types, including HPV16 and 18, is currently used in clinical practice for triage of equivocal cytology and, in conjunction with Pap tests, is an option for general screening among women 30 years of age and older. It is not clear to what extent individual identification of HPV16 or HPV18 as an adjunct to pooled oncogenic HPV testing might effectively identify women at particularly high risk of cervical cancer or its immediate precursor, cervical intraepithelial neoplasia 3 (CIN3). METHODS: From April 1, 1989, to November 2, 1990, a total of 20 810 women in the Kaiser Permanente health plan in Portland, OR, enrolled in a cohort study of HPV and cervical neoplasia. Women were tested for 13 oncogenic HPV types by Hybrid Capture 2 (HC2), and those women with a positive HC2 test were tested for HPV16 and 18. Enrollment Pap smear interpretation and HPV test results were linked to histologically confirmed CIN3 and cervical cancer ( greater than or equal to CIN3) occurring during 10 years of cytologic follow-up. We calculated cumulative incidence rates with 95% confidence intervals for each interval up to 122 months using Kaplan-Meier methods. RESULTS: The 10-year cumulative incidence rates of greater than or equal to CIN3 were 17.2% (95% confidence interval [CI] = 11.5% to 22.9%) among HPV16+ women and 13.6% (95% CI = 3.6% to 23.7%) among HPV18+ (HPV16-) women, but only 3.0% (95% CI = 1.9% to 4.2%) among HC2+ women negative for HPV16 or HPV18. The 10-year cumulative incidence among HC2- women was 0.8% (95% CI = 0.6% to 1.1%). A subanalysis among women 30 years of age and older with normal cytology at enrollment strengthened the observed risk differences. CONCLUSIONS: HPV screening that distinguishes HPV16 and HPV18 from other oncogenic HPV types may identify women at the greatest risk of greater than or equal to CIN3 and may permit less aggressive management of other women with oncogenic HPV infections. JF - Journal of the National Cancer Institute AU - Khan, Michelle J AU - Castle, Philip E AU - Lorincz, Attila T AU - Wacholder, Sholom AU - Sherman, Mark AU - Scott, David R AU - Rush, Brenda B AU - Glass, Andrew G AU - Schiffman, Mark AD - Hormonal and Reproductive Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD (MJK, PEC, SW, M. Sherman, M. Schiffman) Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1072 EP - 1079 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 97 IS - 14 SN - 0027-8874, 0027-8874 KW - Risk Abstracts; Virology & AIDS Abstracts; Oncogenes & Growth Factors Abstracts KW - Risk assessment KW - Age KW - USA, Oregon, Portland KW - Cervical cancer KW - Human papillomavirus 18 KW - Clinical aspects KW - Infection KW - Cancer KW - Neoplasia KW - hybrids KW - Human papillomavirus 16 KW - Risk factors KW - infection KW - Geriatrics KW - Human papilloma virus 18 KW - Females KW - Cervix KW - Human papilloma virus 16 KW - Human papillomavirus KW - R2 23060:Medical and environmental health KW - V 22114:Human oncogenic viruses KW - B 26250:Papovaviruses UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20719259?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=The+Elevated+10-Year+Risk+of+Cervical+Precancer+and+Cancer+in+Women+With+Human+Papillomavirus+%28HPV%29+Type+16+or+18+and+the+Possible+Utility+of+Type-Specific+HPV+Testing+in+Clinical+Practice&rft.au=Khan%2C+Michelle+J%3BCastle%2C+Philip+E%3BLorincz%2C+Attila+T%3BWacholder%2C+Sholom%3BSherman%2C+Mark%3BScott%2C+David+R%3BRush%2C+Brenda+B%3BGlass%2C+Andrew+G%3BSchiffman%2C+Mark&rft.aulast=Khan&rft.aufirst=Michelle&rft.date=2005-07-01&rft.volume=97&rft.issue=14&rft.spage=1072&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Risk assessment; Risk factors; Cervical cancer; Geriatrics; Infection; Clinical aspects; Cervix; Neoplasia; Age; hybrids; infection; Females; Cancer; Human papillomavirus 16; Human papillomavirus 18; Human papilloma virus 18; Human papillomavirus; Human papilloma virus 16; USA, Oregon, Portland ER - TY - JOUR T1 - Respiratory and general health impairments of workers employed in a municipal solid waste disposal at an open landfill site in Delhi AN - 20718501; 6820339 AB - The objective of this study was to examine the respiratory and general health of workers employed in a municipal solid waste (MSW) disposal at an open landfill site in India. Ninety-six landfill workers of Okhla landfill site, Delhi, and 90 controls matched for age, sex, and socioeconomic conditions were enrolled. Health data was obtained from questionnaire surveys, clinical examination and laboratory investigations. Lung function was evaluated by spirometry. Compared with matched controls, landfill workers had significantly higher prevalences of both upper and lower respiratory symptoms, and they suffered more often from diarrhea, fungal infection and ulceration of the skin, burning sensation in the extremities, tingling or numbness, transient loss of memory, and depression. Spirometry revealed impairment of lung function in 62% of the landfill workers compared to 27% of the controls. Sputum cytology showed squamous metaplasia, abundance of inflammatory cells, alveolar macrophages (AM) and siderophages (macrophages with iron deposits), and high elastase enzyme activity in neutrophils and AM of a majority of landfill workers, indicating adverse cellular lung reaction. Hematological profiles of these workers depicted low hemoglobin and erythrocyte levels with high total leukocyte, eosinophil and monocyte counts. Erythrocytes with target cell morphology were abundant in 42% of the landfill workers compared to 10% of the controls. Toxic granulation in neutrophils, an indication of infection and inflammation, was recorded in 94% of the landfill workers and in 49% of the controls. The results demonstrated higher prevalence of respiratory symptoms, inflammation of the airways, lung function decrement and a wide range of general health problems in MSW disposal workers. JF - International Journal of Hygiene and Environmental Health AU - Ray, M R AU - Roychoudhury, S AU - Mukherjee, G AU - Roy, S AU - Lahiri, T AD - Chittaranjan National Cancer Institute, Kolkata Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 255 EP - 262 VL - 208 IS - 4 SN - 1438-4639, 1438-4639 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Toxicology Abstracts; Health & Safety Science Abstracts; Pollution Abstracts KW - Macrophages KW - Landfills KW - Erythrocytes KW - Socioeconomics KW - Leukocytes (eosinophilic) KW - Infection KW - Municipal solid wastes KW - Solid wastes KW - India KW - Extremities KW - Hemoglobin KW - Workers KW - Memory KW - Metaplasia KW - Waste disposal sites KW - Cytology KW - India, Delhi KW - Hematology KW - Respiratory function KW - Monocytes KW - Occupational exposure KW - Respiratory tract KW - Sex KW - Deposits KW - Inventories KW - Depression KW - Diarrhea KW - Skin KW - Data processing KW - Elastase KW - Leukocytes (neutrophilic) KW - Enzymes KW - Toxicity KW - Alveoli KW - Inflammation KW - Socio-economic aspects KW - Lung KW - Burning KW - Sputum KW - Iron KW - X 24240:Miscellaneous KW - H 1000:Occupational Safety and Health KW - P 6000:TOXICOLOGY AND HEALTH KW - K 03420:Plant Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20718501?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Hygiene+and+Environmental+Health&rft.atitle=Respiratory+and+general+health+impairments+of+workers+employed+in+a+municipal+solid+waste+disposal+at+an+open+landfill+site+in+Delhi&rft.au=Ray%2C+M+R%3BRoychoudhury%2C+S%3BMukherjee%2C+G%3BRoy%2C+S%3BLahiri%2C+T&rft.aulast=Ray&rft.aufirst=M&rft.date=2005-07-01&rft.volume=208&rft.issue=4&rft.spage=255&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Hygiene+and+Environmental+Health&rft.issn=14384639&rft_id=info:doi/10.1016%2Fj.ijheh.2005.02.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Macrophages; Landfills; Erythrocytes; Leukocytes (eosinophilic); Infection; Solid wastes; Hemoglobin; Workers; Memory; Metaplasia; Cytology; Monocytes; Sex; Respiratory tract; Inventories; Deposits; Data processing; Skin; Diarrhea; Depression; Elastase; Leukocytes (neutrophilic); Enzymes; Alveoli; Inflammation; Socio-economic aspects; Lung; Sputum; Burning; Iron; Extremities; Waste disposal sites; Socioeconomics; Hematology; Toxicity; Respiratory function; Occupational exposure; Municipal solid wastes; India, Delhi; India DO - http://dx.doi.org/10.1016/j.ijheh.2005.02.001 ER - TY - JOUR T1 - Human Papillomavirus Type 16 Infections and 2-Year Absolute Risk of Cervical Precancer in Women With Equivocal or Mild Cytologic Abnormalities AN - 20717631; 6477436 AB - BACKGROUND: The 2-year absolute risk for cervical precancer attributable to infection by human papillomavirus type 16 (HPV16), the most common and oncogenic HPV type, in the millions of women diagnosed annually with equivocal or mildly abnormal cytology has not been definitively evaluated. METHODS: Baseline cervical specimens of 5060 women with equivocal (atypical squamous cells of undetermined significance [ASCUS]) or mildly abnormal (low-grade squamous intraepithelial lesion [LSIL]) cytology were tested for HPV DNA using Hybrid Capture 2 (HC2) and type-specific L1 consensus primer polymerase chain reaction. We calculated absolute risks with 95% confidence intervals (CIs) for cumulative diagnosis, during the 2-year study period, of cervical intraepithelial neoplasia grade 3 (CIN3) (n = 535) or cancer (n = 7) (collectively referred to as greater than or equal to CIN3) and compared risk by HPV16 status and by other oncogenic HPV types using logistic regression. All statistical tests were two-sided. RESULTS: The baseline prevalences of HPV16 in women with ASCUS or LSIL cytology were 14.9% and 21.1%, respectively. Women with ASCUS or LSIL cytology who were HPV16 DNA positive at baseline had 2-year cumulative absolute risks for greater than or equal to CIN3 of 32.5% (95% CI = 28.4% to 36.8%) and 39.1% (95% CI = 33.8% to 44.7%), respectively. By comparison, women with ASCUS who were positive by HC2 for other oncogenic HPV types combined had an 8.4% (95% CI = 6.9% to 10.4%) risk for greater than or equal to CIN3, which was similar to the risk posed by having ASCUS (risk = 8.8%, 95% CI = 7.9% to 9.8%) without knowledge of the oncogenic HPV DNA status. Women with LSILs who were positive by HC2 for other oncogenic HPV types combined had a 9.9% (95% CI = 8.0% to 12.0%) 2-year risk for greater than or equal to CIN3, which was less than the risk posed by having LSILs (risk = 15.0%, 95% CI = 13.3% to 16.9%) without knowledge of the oncogenic HPV DNA status. Together, women with ASCUS or LSILs who were HPV16-positive had the highest 2-year risk for greater than or equal to CIN3 compared with women who were HPV-negative (odds ratio [OR] = 38, 95% CI = 22 to 68; P<.001 ), fivefold greater than the increased risk in women who were positive for other oncogenic HPV types (OR = 7.2, 95%CI = 4.2 to 13, P<.001). CONCLUSIONS: Distinguishing the high absolute risk for cervical precancer in HPV16-positive women from the lower risk posed by other oncogenic HPV types might have clinical implications. JF - Journal of the National Cancer Institute AU - Castle, Philip E AU - Solomon, Diane AU - Schiffman, Mark AU - Wheeler, Cosette M AD - Division of Cancer Epidemiology and Genetics (PEC, MS) and Division of Cancer Prevention (DS), National Cancer Institute, NIH, DHHS, Bethesda, MD Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1066 EP - 1071 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 97 IS - 14 SN - 0027-8874, 0027-8874 KW - Risk Abstracts; Virology & AIDS Abstracts; Oncogenes & Growth Factors Abstracts KW - Squamous cells KW - Cervical cancer KW - Statistical analysis KW - Infection KW - Cancer KW - Neoplasia KW - hybrids KW - Human papillomavirus 16 KW - Risk factors KW - DNA KW - infection KW - Lesions KW - Polymerase chain reaction KW - Primers KW - Females KW - Cervix KW - Human papilloma virus 16 KW - R2 23060:Medical and environmental health KW - V 22114:Human oncogenic viruses KW - B 26250:Papovaviruses UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20717631?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Human+Papillomavirus+Type+16+Infections+and+2-Year+Absolute+Risk+of+Cervical+Precancer+in+Women+With+Equivocal+or+Mild+Cytologic+Abnormalities&rft.au=Castle%2C+Philip+E%3BSolomon%2C+Diane%3BSchiffman%2C+Mark%3BWheeler%2C+Cosette+M&rft.aulast=Castle&rft.aufirst=Philip&rft.date=2005-07-01&rft.volume=97&rft.issue=14&rft.spage=1066&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Risk factors; Cervical cancer; Squamous cells; Statistical analysis; Polymerase chain reaction; Primers; Infection; Cervix; Neoplasia; Cancer; hybrids; infection; DNA; Lesions; Females; Human papillomavirus 16; Human papilloma virus 16 ER - TY - JOUR T1 - Phosphorimaging detection and quantitation for isotopic ion flux assays AN - 20297252; 7536538 AB - A 96-well-microplate-based ion flux method utilizing readily available autoradiographic phosphorimaging detection is described. Nicotinic acetylcholine receptor-mediated 22Na influx in four cultured cell lines provided satisfactory concentration-response data for epibatidine and several other nicotinic agonists. The data were consistent with data obtained using standard 6-well assays. Assays for nicotinic-receptor-mediated 86Rb efflux produced data similar to data obtained with the 22Na influx assay. However, assays for 45Ca influx were not successful, although 45Ca was readily detected and quantified. Voltage-gated sodium channel-mediated 22Na influx in a neuroblastoma cell line allowed assay of the effects of such sodium channel activators as batrachotoxin and a pumiliotoxin B/scorpion venom combination. Phosphorimaging detection allows for reliable beta counting of up to 1200 simultaneous samples with excellent sensitivity and is amenable for application to high-throughput screening. JF - Analytical Biochemistry AU - Fitch, Richard W AU - Daly, John W AD - Section on Drug-Receptor Interactions, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA, rfitch@indstate.edu Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 260 EP - 270 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 342 IS - 2 SN - 0003-2697, 0003-2697 KW - Biotechnology and Bioengineering Abstracts KW - Nicotine KW - Acetylcholine receptor KW - Epibatidine KW - Radioisotope tracing KW - Sodium channel KW - Sodium influx KW - Rubidium efflux KW - Batrachotoxin KW - Autoradiography KW - Data processing KW - Sodium channels (voltage-gated) KW - Neuroblastoma cells KW - high-throughput screening KW - Enumeration KW - Venom KW - Quantitation KW - epibatidine KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20297252?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Analytical+Biochemistry&rft.atitle=Phosphorimaging+detection+and+quantitation+for+isotopic+ion+flux+assays&rft.au=Fitch%2C+Richard+W%3BDaly%2C+John+W&rft.aulast=Fitch&rft.aufirst=Richard&rft.date=2005-07-01&rft.volume=342&rft.issue=2&rft.spage=260&rft.isbn=&rft.btitle=&rft.title=Analytical+Biochemistry&rft.issn=00032697&rft_id=info:doi/10.1016%2Fj.ab.2005.04.041 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Sodium channels (voltage-gated); Data processing; Neuroblastoma cells; high-throughput screening; Enumeration; Venom; Quantitation; epibatidine DO - http://dx.doi.org/10.1016/j.ab.2005.04.041 ER - TY - JOUR T1 - Plant Genome Resources at the National Center for Biotechnology Information AN - 20225419; 6477591 AB - The National Center for Biotechnology Information (NCBI) integrates data from more than 20 biological databases through a flexible search and retrieval system called Entrez. A core Entrez database, Entrez Nucleotide, includes GenBank and is tightly linked to the NCBI Taxonomy database, the Entrez Protein database, and the scientific literature in PubMed. A suite of more specialized databases for genomes, genes, gene families, gene expression, gene variation, and protein domains dovetails with the core databases to make Entrez a powerful system for genomic research. Linked to the full range of Entrez databases is the NCBI Map Viewer, which displays aligned genetic, physical, and sequence maps for eukaryotic genomes including those of many plants. A specialized plant query page allow maps from all plant genomes covered by the Map Viewer to be searched in tandem to produce a display of aligned maps from several species. PlantBLAST searches against the sequences shown in the Map Viewer allow BLAST alignments to be viewed within a genomic context. In addition, precomputed sequence similarities, such as those for proteins offered by BLAST Link, enable fluid navigation from unannotated to annotated sequences, quickening the pace of discovery. NCBI Web pages for plants, such as Plant Genome Central, complete the system by providing centralized access to NCBI's genomic resources as well as links to organism-specific Web pages beyond NCBI. JF - Plant Physiology AU - Wheeler, David L AU - Smith-White, Brian AU - Chetvernin, Vyacheslav AU - Resenchuk, Sergei AU - Dombrowski, Susan M AU - Pechous, Steven W AU - Tatusova, Tatiana AU - Ostell, James AD - National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20894 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1280 EP - 1288 PB - American Society of Plant Biologists, 15501 Monona Dr. Rockville MD 20855-2768 USA, [mailto:mjunior@aspp.org], [URL:http://www.aspb.org] VL - 138 IS - 3 SN - 0032-0889, 0032-0889 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Databases KW - Data processing KW - Taxonomy KW - genomics KW - Nucleotides KW - Gene mapping KW - G 07800:Plants and Algae KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20225419?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Plant+Physiology&rft.atitle=Plant+Genome+Resources+at+the+National+Center+for+Biotechnology+Information&rft.au=Wheeler%2C+David+L%3BSmith-White%2C+Brian%3BChetvernin%2C+Vyacheslav%3BResenchuk%2C+Sergei%3BDombrowski%2C+Susan+M%3BPechous%2C+Steven+W%3BTatusova%2C+Tatiana%3BOstell%2C+James&rft.aulast=Wheeler&rft.aufirst=David&rft.date=2005-07-01&rft.volume=138&rft.issue=3&rft.spage=1280&rft.isbn=&rft.btitle=&rft.title=Plant+Physiology&rft.issn=00320889&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Genomes; Databases; Data processing; Taxonomy; genomics; Nucleotides; Gene mapping ER - TY - JOUR T1 - Micros for microbes: non-coding regulatory RNAs in bacteria AN - 20005502; 8251503 AB - Small non-coding RNAs with important regulatory roles are not confined to eukaryotes. Recent studies have led to the identification of numerous small regulatory RNAs in Escherichia coli and in other bacteria. As in eukaryotic cells, a major class of these small RNAs acts by base-pairing with target mRNAs, resulting in changes in the translation and stability of the mRNA. Roles for these non-coding pairing RNAs in bacteria have been demonstrated in several cases. Because these non-coding RNAs act post-transcriptionally, they impose a regulatory step that is independent of and epistatic to any transcriptional signals for their target mRNAs. JF - Trends in Genetics AU - Gottesman, S Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 399 EP - 404 PB - Elsevier Science, The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 21 IS - 7 SN - 0168-9525, 0168-9525 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Translation KW - mRNA stability KW - Epistasis KW - Reviews KW - Escherichia coli KW - non-coding RNA KW - Transcription KW - Post-transcription KW - J 02490:Miscellaneous KW - G 07770:Bacteria KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20005502?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Trends+in+Genetics&rft.atitle=Micros+for+microbes%3A+non-coding+regulatory+RNAs+in+bacteria&rft.au=Gottesman%2C+S&rft.aulast=Gottesman&rft.aufirst=S&rft.date=2005-07-01&rft.volume=21&rft.issue=7&rft.spage=399&rft.isbn=&rft.btitle=&rft.title=Trends+in+Genetics&rft.issn=01689525&rft_id=info:doi/10.1016%2Fj.tig.2005.05.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Translation; mRNA stability; Reviews; Epistasis; non-coding RNA; Transcription; Post-transcription; Escherichia coli DO - http://dx.doi.org/10.1016/j.tig.2005.05.008 ER - TY - JOUR T1 - Antidepressant-elicited changes in gene expression. Remodeling of neuronal circuits as a new hypothesis for drug efficacy AN - 19950065; 6656622 AB - Although antidepressants have been used clinically for more than 50 years, no consensus has been reached concerning their precise molecular mechanism of action. Pharmacogenomics is a powerful tool that can be used to identify genes affected by antidepressants or by other effective therapeutic manipulations. Using this tool, others and we have identified as candidate molecular targets several genes or expressed sequence tags (ESTs) that are induced by chronic antidepressant treatment. In this article, we review antidepressant-elicited changes in gene expression, focusing especially on the remodeling of neuronal circuits that results. This refocusing motivates our hypothesis that this plasticity represents the mechanism for drug efficacy, and thus a causal event for clinical improvement. Defining the roles of these molecules in drug-induced neural plasticity is likely to transform the course of research on the biological basis of antidepressants. Such detailed knowledge will have profound effects on the diagnosis, prevention, and treatment of depression. Consideration of novel biological approaches beyond the "monoamine hypothesis" of depression is expected to evoke paradigm shifts in the future of antidepressant research. JF - Progress in Neuro-Psychopharmacology and Biological Psychiatry AU - Yamada, Mitsuhiko AU - Yamada, Misa AU - Higuchi, Teruhiko AD - Department of Psychogeriatrics, National Institute of Mental Health, National Center of Neurology and Psychiatry, Tokyo 187-8553, Japan, mitsu@ncnp-k.go.jp Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 999 EP - 1009 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 29 IS - 6 SN - 0278-5846, 0278-5846 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Antidepressant KW - Depression KW - Microarray KW - Neural plasticity KW - Pharmacogenomics KW - Gene expression KW - Antidepressants KW - Molecular modelling KW - monoamines KW - pharmacogenomics KW - Reviews KW - Plasticity (neural) KW - Circuits KW - expressed sequence tags KW - Drugs KW - W 30925:Genetic Engineering KW - N3 11001:Behavioral and Cognitive Neuroscience KW - G 07780:Fungi UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19950065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+Neuro-Psychopharmacology+and+Biological+Psychiatry&rft.atitle=Antidepressant-elicited+changes+in+gene+expression.+Remodeling+of+neuronal+circuits+as+a+new+hypothesis+for+drug+efficacy&rft.au=Yamada%2C+Mitsuhiko%3BYamada%2C+Misa%3BHiguchi%2C+Teruhiko&rft.aulast=Yamada&rft.aufirst=Mitsuhiko&rft.date=2005-07-01&rft.volume=29&rft.issue=6&rft.spage=999&rft.isbn=&rft.btitle=&rft.title=Progress+in+Neuro-Psychopharmacology+and+Biological+Psychiatry&rft.issn=02785846&rft_id=info:doi/10.1016%2Fj.pnpbp.2005.03.022 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Gene expression; Molecular modelling; Antidepressants; monoamines; Depression; pharmacogenomics; Reviews; Plasticity (neural); Circuits; Drugs; expressed sequence tags DO - http://dx.doi.org/10.1016/j.pnpbp.2005.03.022 ER - TY - JOUR T1 - Quality of Parental Consent in a Ugandan Malaria Study AN - 19845347; 6478133 AB - OBJECTIVES: We surveyed Ugandan parents who enrolled their children in a randomized pediatric malaria treatment trial to evaluate the parents' levels of understanding about the treatment trial and the quality of the parents' consents to allow their children to participate in the study. METHODS: We conducted 347 interviews immediately following enrollment at 4 Ugandan sites. RESULTS: A majority (78%) of the parents, most of whom where mothers (86%) had at most a primary school education. Of the participating mothers, a substantial percentage reported that they remembered being told about the study's purpose (77%), the required number of visits (88%), the risks involved (61%), treatment allocation (84%), and their ability to discontinue their children's participation (64%). In addition, most reported knowing the trial's purpose (80%) and the required number of visits (78%); however, only 18% could name possible side effects from the drugs being administered, and only 19% knew that children would not all be administered identical treatments. Ninety-four percent reported that they made the enrollment decision themselves, but 58% said they felt pressure to participate because of their child's illness, and 15% said they felt some type of pressure to participate from others; 41% reported knowing that they did not have to participate. CONCLUSIONS: The consent Ugandan parents provided to allow their children to participate in the malaria study was of mixed quality. Parents understood many of the study details, but they were not very aware of the risks involved or of randomization. Many parents felt that they could not have refused to participate because their child was sick and they either did not know or did not believe that their child would receive treatment outside of the study. Our results indicate that further debate is needed about informed consent in treatment studies of emergent illnesses in children. JF - American Journal of Public Health AU - Pace, Christine AU - Talisuna, Ambrose AU - Wendler, David AU - Maiso, Faustin AU - Wabwire-Mangen, Fred AU - Bakyaita, Nathan AU - Okiria, Edith AU - Garrett-Mayer, Elizabeth S AU - Emanuel, Ezekiel AU - Grady, Christine AD - At the time of this study, Christine Pace was with the Department of Clinical Bioethics, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Md. David Wendler, Ezekiel Emanuel, and Christine Grady are with the Department of Clinical Bioethics, Warren G. Magnuson Clinical Center, National Institutes of Health. Ambrose Talisuna and Faustin Maiso are with the Uganda Malaria Surveillance Project, Ministry of Health, Kampala, Uganda. Fred Wabwire-Mangen and Nathan Bakyaita are with the Uganda Malaria Surveillance Project, Makerere University, Kampala. Edith Okiria is with the Department of Women and Gender Studies, Makerere University. Elizabeth S. Garrett-Mayer is with the Department of Oncology, Division of Biostatistics, Johns Hopkins School of Medicine, Baltimore, Md Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1184 EP - 1189 PB - American Public Health Association, 1015 15th St., N.W. Washington DC 20005 USA VL - 95 IS - 7 SN - 0090-0036, 0090-0036 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Health & Safety Science Abstracts KW - Pediatrics KW - Malaria KW - Children KW - Public health KW - Education KW - schools KW - malaria KW - Pressure KW - Drugs KW - Side effects KW - K 03400:Human Diseases KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19845347?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Public+Health&rft.atitle=Quality+of+Parental+Consent+in+a+Ugandan+Malaria+Study&rft.au=Pace%2C+Christine%3BTalisuna%2C+Ambrose%3BWendler%2C+David%3BMaiso%2C+Faustin%3BWabwire-Mangen%2C+Fred%3BBakyaita%2C+Nathan%3BOkiria%2C+Edith%3BGarrett-Mayer%2C+Elizabeth+S%3BEmanuel%2C+Ezekiel%3BGrady%2C+Christine&rft.aulast=Pace&rft.aufirst=Christine&rft.date=2005-07-01&rft.volume=95&rft.issue=7&rft.spage=1184&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Public+Health&rft.issn=00900036&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Pediatrics; Malaria; Pressure; Children; Drugs; Side effects; Public health; Education; schools; malaria ER - TY - JOUR T1 - Chromogranin A Deficiency in Transgenic Mice Leads to Aberrant Chromaffin Granule Biogenesis AN - 19844070; 6479153 AB - The biogenesis of dense-core secretory granules (DCGs), organelles responsible for the storage and secretion of neurotransmitters and neuropeptides in chromaffin cells, is poorly understood. Chromogranin A (CgA), which binds catecholamines for storage in the lumen of chromaffin granules, has been shown to be involved in DCG biogenesis in neuroendocrine PC12 cells. Here, we report that downregulation of CgA expression in vivo by expressing antisense RNA against CgA in transgenic mice led to a significant reduction in DCG formation in adrenal chromaffin cells. The number of DCGs formed in CgA antisense transgenic mice was directly correlated with the amount of CgA present in adrenal medulla. In addition, DCGs showed an increase in size, with enlargement in the volume around the dense core, a phenomenon that occurs to maintain constant "free" catecholamine concentration in the lumen of these granules. The extent of DCG swelling was inversely correlated with the number of DCGs formed, as well as the amount of CgA present in the adrenal glands of CgA antisense transgenic mice. These data indicate an essential role of CgA in regulating chromaffin DCG biogenesis and catecholamine storage in vivo. JF - Journal of Neuroscience AU - Kim, Taeyoon AU - Zhang, Chun-fa AU - Sun, Ziqing AU - Wu, Heling AU - Loh, YPeng AD - Section on Cellular Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, and College of Biology, Peking University, 100871 Beijing, China Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 6958 EP - 6961 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 30 SN - 0270-6474, 0270-6474 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Granules KW - Adrenal glands KW - chromogranins KW - Data processing KW - Secretion KW - Antisense RNA KW - Transgenic mice KW - Adrenal medulla KW - Nervous system KW - Chromaffin granules KW - Pheochromocytoma cells KW - Catecholamines KW - Secretory vesicles KW - Dense core vesicles KW - Chromaffin cells KW - Neurotransmitters KW - Organelles KW - Neuropeptides KW - W 30925:Genetic Engineering KW - N3 11080:Neuroendocrinology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19844070?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Chromogranin+A+Deficiency+in+Transgenic+Mice+Leads+to+Aberrant+Chromaffin+Granule+Biogenesis&rft.au=Kim%2C+Taeyoon%3BZhang%2C+Chun-fa%3BSun%2C+Ziqing%3BWu%2C+Heling%3BLoh%2C+YPeng&rft.aulast=Kim&rft.aufirst=Taeyoon&rft.date=2005-07-01&rft.volume=25&rft.issue=30&rft.spage=6958&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-02-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - chromogranins; Adrenal glands; Granules; Data processing; Secretion; Antisense RNA; Transgenic mice; Adrenal medulla; Chromaffin granules; Nervous system; Catecholamines; Pheochromocytoma cells; Secretory vesicles; Dense core vesicles; Chromaffin cells; Neurotransmitters; Organelles; Neuropeptides ER - TY - JOUR T1 - Effects of interleukin-15 on antifungal responses of human polymorphonuclear leukocytes against Fusarium spp. and Scedosporium spp. super() AN - 19825606; 6453217 AB - Fusarium spp. and Scedosporium spp. have emerged as important fungal pathogens that are frequently resistant to antifungal compounds. We investigated the effects of human interleukin-15 (IL-15) on human polymorphonuclear leukocyte (PMNL) activity against Fusarium solani and Fusarium oxysporum as well as Scedosporium prolificans and Scedosporium apiospermum. IL-15 (100 ng/ml) significantly enhanced PMNL-induced hyphal damage of both Fusarium spp. and S. prolificans after incubation for 22 h (, P<0.01, ) but not S. apiospermum. In addition, IL-15 enhanced PMNL oxidative respiratory burst evaluated as superoxide anion production in response to S. prolificans but not to the other fungi after 2 h incubation. IL-15 increased interleukin-8 (IL-8) release from PMNLs challenged with hyphae of F. solani and S. prolificans (, P[precedesorequalto]0.04, ). Release of tumor necrosis factor- alpha was not affected. The species-dependent enhancement of hyphal damage and induction of IL-8 release suggest that IL-15 plays an important role in the immunomodulation of host response to these emerging fungal pathogens. JF - Cytokine AU - Winn, R M AU - Gil-Lamaignere, C AU - Roilides, E AU - Simitsopoulou, M AU - Lyman, CA AU - Maloukou, A AU - Walsh, T J AD - 3rd Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, GR-54642 Thessaloniki, Greece, walsht@mail.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1 EP - 8 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 31 IS - 1 SN - 1043-4666, 1043-4666 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Microbiology Abstracts A: Industrial & Applied Microbiology; Immunology Abstracts KW - Filamentous fungi KW - Hyphal damage KW - Interleukin-15 KW - Polymorphonuclear leukocytes KW - Oxidative burst KW - Scedosporium apiospermum KW - Antifungal agents KW - Leukocytes (polymorphonuclear) KW - Fungi KW - Hyphae KW - Fusarium oxysporum KW - Pathogens KW - Immunomodulation KW - Interleukin 8 KW - Respiratory burst KW - Interleukin 15 KW - superoxide anions KW - Tumor necrosis factor- alpha KW - Scedosporium KW - Scedosporium prolificans KW - Fusarium solani KW - K 03340:Effects of Physical & Chemical Factors KW - F 06108:Fungi KW - A 01340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19825606?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologya&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cytokine&rft.atitle=Effects+of+interleukin-15+on+antifungal+responses+of+human+polymorphonuclear+leukocytes+against+Fusarium+spp.+and+Scedosporium+spp.+super%28%29&rft.au=Winn%2C+R+M%3BGil-Lamaignere%2C+C%3BRoilides%2C+E%3BSimitsopoulou%2C+M%3BLyman%2C+CA%3BMaloukou%2C+A%3BWalsh%2C+T+J&rft.aulast=Winn&rft.aufirst=R&rft.date=2005-07-01&rft.volume=31&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Cytokine&rft.issn=10434666&rft_id=info:doi/10.1016%2Fj.cyto.2004.07.016 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Antifungal agents; Respiratory burst; Interleukin 15; Fungi; Leukocytes (polymorphonuclear); Hyphae; superoxide anions; Tumor necrosis factor- alpha; Pathogens; Immunomodulation; Interleukin 8; Scedosporium apiospermum; Fusarium oxysporum; Scedosporium prolificans; Scedosporium; Fusarium solani DO - http://dx.doi.org/10.1016/j.cyto.2004.07.016 ER - TY - JOUR T1 - Algorithms for alignment of mass spectrometry proteomic data AN - 19698033; 6429152 AB - MOTIVATION: The analysis of biological samples with high-throughput mass spectrometers has increased greatly in recent years. As larger datasets are processed, it is important that the spectra are aligned to ensure that the same protein intensities are correctly identified in each sample. Without such an alignment procedure it is possible to make errors in identifying the signals from peptides with similar molecular weight. Two algorithms are provided that can improve the alignment among samples. One algorithm is designed to work with SELDI data produced from a Ciphergen instrument, and the other can be used with data in a more general format. RESULTS: The two algorithms were applied to samples drawn from a common pool of reference serum. The results indicate substantial improvement in consistently identifying peptide signals in different samples. AVAILABILITY: The two algorithms are programmed using the R language and are available from http://krisa.ninds.nih.gov/alignment/ JF - Bioinformatics AU - Jeffries, Neal AD - National Institute of Neurological Disorders and Stroke, National Institutes of Health Bethesda, MD 20892, USA Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 3066 EP - 3073 PB - Oxford University Press, [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 14 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Molecular weight KW - Algorithms KW - proteomics KW - Bioinformatics KW - Mass spectroscopy KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19698033?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Algorithms+for+alignment+of+mass+spectrometry+proteomic+data&rft.au=Jeffries%2C+Neal&rft.aulast=Jeffries&rft.aufirst=Neal&rft.date=2005-07-01&rft.volume=21&rft.issue=14&rft.spage=3066&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Algorithms; Bioinformatics; Molecular weight; proteomics; Mass spectroscopy ER - TY - JOUR T1 - Influence of proton T sub(1) on oxymetry using Overhauser enhanced magnetic resonance imaging AN - 19416658; 6489002 AB - In Overhauser enhanced magnetic resonance imaging (OMRI) for in vivo measurement of oxygen partial pressure (pO sub(2)), a paramagnetic contrast agent is introduced to enhance the proton signal through dynamic nuclear polarization. A uniform proton T sub(1) is generally assumed for the entire region of interest for the computation of pO sub(2) using OMRI. It is demonstrated here, by both phantom and in vivo (mice) imaging, that such an assumption may cause erroneous estimate of pO sub(2). A direct estimate of pixel-wise T sub(1) is hampered by the poor native MR intensities, owing to the very low Zeeman field (15-20 mT) in OMRI. To circumvent this problem, a simple method for the pixel-wise mapping of proton T sub(1) using the OMRI scanner is described. A proton T sub(1) image of a slice through the center of an SCC tumor in a mouse clearly shows a range of T sub(1) distribution (0.2 similar to 1.6 s). Computation of pO sub(2) images using pixel-wise T sub(1) values promises oximetry with minimal artifacts by OMRI. JF - Magnetic Resonance in Medicine AU - Matsumoto, Shingo AU - Utsumi, Hideo AU - Aravalluvan, Thirumaran AU - Matsumoto, Ken-Ichiro AU - Matsumoto, Atsuko AU - Devasahayam, Nallathamby AU - Sowers, Anastasia L AU - Mitchell, James B AU - Subramanian, Sankaran AU - Krishna, Murali C AD - Building 10, Room B3B69, NIH, Bethesda, MD 20892-1002, USA, murali@helix.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 213 EP - 217 PB - John Wiley & Sons, Ltd. VL - 54 IS - 1 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Oxygen KW - Protons KW - Magnetic resonance imaging KW - Contrast media KW - Image processing KW - Tumors KW - Pressure KW - Polarization KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19416658?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Influence+of+proton+T+sub%281%29+on+oxymetry+using+Overhauser+enhanced+magnetic+resonance+imaging&rft.au=Matsumoto%2C+Shingo%3BUtsumi%2C+Hideo%3BAravalluvan%2C+Thirumaran%3BMatsumoto%2C+Ken-Ichiro%3BMatsumoto%2C+Atsuko%3BDevasahayam%2C+Nallathamby%3BSowers%2C+Anastasia+L%3BMitchell%2C+James+B%3BSubramanian%2C+Sankaran%3BKrishna%2C+Murali+C&rft.aulast=Matsumoto&rft.aufirst=Shingo&rft.date=2005-07-01&rft.volume=54&rft.issue=1&rft.spage=213&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20564 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Protons; Magnetic resonance imaging; Contrast media; Image processing; Polarization; Tumors; Oxygen; Pressure DO - http://dx.doi.org/10.1002/mrm.20564 ER - TY - JOUR T1 - Manganese enhanced magnetic resonance imaging of normal and ischemic canine heart AN - 19411375; 6488999 AB - The ability of MnCl sub(2) to enhance canine myocardium and to delineate ischemic areas is demonstrated. A dose-response curve was measured using T sub(1) weighted images in 11 dogs. MnCl sub(2) (36, 113, 360, and 3600 mu mol) was infused over a period of 3 min. Signal intensity increased linearly with MnCl sub(2) dose. At 113 mu mol ( similar to 10 mu mol/kg) the steady-state increase in intensity averaged 212 plus or minus 34%. No significant physiologic effects due to the infused MnCl sub(2) were detected except at the highest dose where there was a cardiac depressive effect. Ischemia was induced by occluding the left anterior descending coronary artery in 5 dogs. At an infused dose of 113 mu mol, MnCl sub(2) clearly demarcated the ischemic zone during coronary occlusion. Contrast enhancement in the ischemic zone was less than 30% compared with normal tissue (P < 0.03). In conclusion, the intracellular contrast agent MnCl sub(2) enhances the canine heart and shows promise in detecting ischemia at doses that do not cause adverse cardiac effects. JF - Magnetic Resonance in Medicine AU - Hu, Tom C-C AU - Christian, Timothy F AU - Aletras, Anthony H AU - Taylor, Joni L AU - Koretsky, Alan P AU - Arai, Andrew E AD - Laboratory of Cardiac Energetics/NHLBI, National Institutes of Health, Building 10, Room B1D416 MSC 1061, Bethesda, MD 20892-1061, USA, araia@nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 196 EP - 200 PB - John Wiley & Sons, Ltd. VL - 54 IS - 1 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Heart KW - Occlusion KW - Magnetic resonance imaging KW - Contrast media KW - N.M.R. KW - Ischemia KW - Manganese KW - Myocardium KW - coronary artery KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19411375?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Manganese+enhanced+magnetic+resonance+imaging+of+normal+and+ischemic+canine+heart&rft.au=Hu%2C+Tom+C-C%3BChristian%2C+Timothy+F%3BAletras%2C+Anthony+H%3BTaylor%2C+Joni+L%3BKoretsky%2C+Alan+P%3BArai%2C+Andrew+E&rft.aulast=Hu&rft.aufirst=Tom&rft.date=2005-07-01&rft.volume=54&rft.issue=1&rft.spage=196&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20516 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Ischemia; Heart; N.M.R.; Contrast media; Manganese; Occlusion; coronary artery; Myocardium; Magnetic resonance imaging DO - http://dx.doi.org/10.1002/mrm.20516 ER - TY - JOUR T1 - Correlation of Apoptosis with Comet Formation Induced by Tea Polyphenols in Human Leukemia Cells AN - 17666716; 6539199 AB - Induction of apoptosis is an important approach to cancer control. Apart from morphological changes in cells, apoptosis is characterized by fragmentation of nuclear DNA. The characteristic DNA ladder formation that is observed on gel electrophoresis does not reflect the DNA breakdown in individual cells; contributions from small subpopulations are usually overlooked. On the other hand, alkaline comet assay as measured by single cell gel electrophoresis accurately measures DNA fragmentation at a single cell level. The comet assay was originally developed as a cytogenetic test to measure the genotoxicity of various chemicals. However, the comet image generated by an apoptotic cell is different from that obtained with a cell treated for a short time with a genotoxic agent. In the present study using human leukemic cells, typical apoptotic features such as morphological characteristics, FACS analysis, caspase activation, and expression of apoptosis-related genes as induced by tea polyphenols have been found to correlate with the comet tail formation. It is apparent from the high degree of correlation observed between the comet tail moment and each parameter of apoptosis that the comet assay can accurately reflect the measure of DNA fragmentation and, hence, can be used to detect a cell undergoing apoptosis. JF - Journal of Entomological Science AU - Kundu, T AU - Bhattacharya, R K AU - Siddiqi, M AU - Roy, M AD - Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, Kolkata, India Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 115 EP - 128 VL - 40 IS - 3 SN - 0749-8004, 0749-8004 KW - Entomology Abstracts; Toxicology Abstracts KW - X 24120:Food, additives & contaminants KW - Z 05183:Toxicology & resistance KW - X 24221:Toxicity testing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17666716?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Entomological+Science&rft.atitle=Correlation+of+Apoptosis+with+Comet+Formation+Induced+by+Tea+Polyphenols+in+Human+Leukemia+Cells&rft.au=Kundu%2C+T%3BBhattacharya%2C+R+K%3BSiddiqi%2C+M%3BRoy%2C+M&rft.aulast=Kundu&rft.aufirst=T&rft.date=2005-07-01&rft.volume=40&rft.issue=3&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Journal+of+Entomological+Science&rft.issn=07498004&rft_id=info:doi/10.1615%2FJEnvPathToxOncol.v24.i2.50 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1615/JEnvPathToxOncol.v24.i2.50 ER - TY - JOUR T1 - Differential Distribution and Expression of Panton-Valentine Leucocidin among Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains AN - 17652032; 6478288 AB - Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging threat worldwide. CA-MRSA strains differ from hospital-acquired MRSA strains in their antibiotic susceptibilities and genetic backgrounds. Using several genotyping methods, we clearly define CA-MRSA at the genetic level and demonstrate that the prototypic CA-MRSA strain, MW2, has spread as a homogeneous clonal strain family that is distinct from other CA-MRSA strains. The Panton-Valentine leucocidin (PVL)-encoding genes, lukF and lukS, are prevalent among CA-MRSA strains and have previously been associated with CA-MRSA infections. To better elucidate the role of PVL in the pathogenesis of CA-MRSA, we first analyzed the distribution and expression of PVL among different CA-MRSA strains. Our data demonstrate that PVL genes are differentially distributed among CA-MRSA strains and, when they are present, are always transcribed, albeit with strain-to-strain variability of transcript levels. To directly test whether PVL is critical for the pathogenesis of CA-MRSA, we evaluated the lysis of human polymorphonuclear leukocytes (PMNs) during phagocytic interaction with PVL-positive and PVL-negative CA-MRSA strains. Unexpectedly, there was no correlation between PVL expression and PMN lysis, suggesting that additional virulence factors underlie leukotoxicity and, thus, the pathogenesis of CA-MRSA. JF - Journal of Clinical Microbiology AU - Saied-Salim, Battouli AU - Mathema, Barun AU - Braughton, Kevin AU - Davis, Stacy AU - Sinsimer, Daniel AU - Eisner, William AU - Likhoshvay, Yekaterina AU - DeLeo, Frank R AU - Kreiswirth, Barry N AD - TB Center, Public Health Research Institute, Newark, New Jersey 07103. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, and National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840. and Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 3373 EP - 3379 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 7 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17652032?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Differential+Distribution+and+Expression+of+Panton-Valentine+Leucocidin+among+Community-Acquired+Methicillin-Resistant+Staphylococcus+aureus+Strains&rft.au=Saied-Salim%2C+Battouli%3BMathema%2C+Barun%3BBraughton%2C+Kevin%3BDavis%2C+Stacy%3BSinsimer%2C+Daniel%3BEisner%2C+William%3BLikhoshvay%2C+Yekaterina%3BDeLeo%2C+Frank+R%3BKreiswirth%2C+Barry+N&rft.aulast=Saied-Salim&rft.aufirst=Battouli&rft.date=2005-07-01&rft.volume=43&rft.issue=7&rft.spage=3373&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Analysis of the Escherichia coli Alp Phenotype: Heat Shock Induction in ssrA Mutants AN - 17650286; 6476115 AB - The major phenotypes of lon mutations, UV sensitivity and overproduction of capsule, are due to the stabilization of two substrates, SulA and RcsA. Inactivation of transfer mRNA (tmRNA) (encoded by ssrA), coupled with a multicopy kanamycin resistance determinant, suppressed both lon phenotypes and restored the rapid degradation of SulA. This novel protease activity was named Alp but was never identified further. We report here the identification, mapping, and characterization of a chromosomal mutation, faa (for function affecting Alp), that leads to full suppression of a Delta lon ssrA::cat host and thus bypasses the requirement for multicopy Kan super(r); faa and ssrA mutants are additive in their ability to suppress lon mutants. The faa mutation was mapped to the C terminus of dnaJ(G232); dnaJ null mutants have similar effects. The identification of a lon suppressor in dnaJ suggested the possible involvement of heat shock. We find that ssrA mutants alone significantly induce the heat shock response. The suppression of UV sensitivity, both in the original Alp strain and in faa mutants, is reversed by mutations in clpY, encoding a subunit of the heat shock-induced ClpYQ protease that is known to degrade SulA. However, capsule synthesis is not restored by clpY mutants, probably because less RcsA accumulates in the Alp strain and because the RcsA that does accumulate is inactive. Both ssrA effects are partially relieved by ssrA derivatives encoding protease-resistant tags, implicating ribosome stalling as the primary defect. Thus, ssrA and faa each suppress two lon mutant phenotypes but by somewhat different mechanisms, with heat shock induction playing a major role. JF - Journal of Bacteriology AU - Munavar, Hussain AU - Zhou, YanNing AU - Gottesman, Susan AD - Bldg. 37, Rm. 5132, Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892-4255. Department of Molecular Biology, School of Biological Sciences, Centre for Excellence in Genomic Sciences, Madurai Kamaraj University, Madurai 625021, India Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 4739 EP - 4751 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 14 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - G 07320:Bacterial genetics KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17650286?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Analysis+of+the+Escherichia+coli+Alp+Phenotype%3A+Heat+Shock+Induction+in+ssrA+Mutants&rft.au=Munavar%2C+Hussain%3BZhou%2C+YanNing%3BGottesman%2C+Susan&rft.aulast=Munavar&rft.aufirst=Hussain&rft.date=2005-07-01&rft.volume=187&rft.issue=14&rft.spage=4739&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - A Human T-Cell Leukemia Virus Type 1 Regulatory Element Enhances the Immunogenicity of Human Immunodeficiency Virus Type 1 DNA Vaccines in Mice and Nonhuman Primates AN - 17648778; 6476923 AB - Plasmid DNA vaccines elicit potent and protective immune responses in numerous small-animal models of infectious diseases. However, their immunogenicity in primates appears less potent. Here we investigate a novel approach that optimizes regulatory elements in the plasmid backbone to improve the immunogenicity of DNA vaccines. Among various regions analyzed, we found that the addition of a regulatory sequence from the R region of the long terminal repeat from human T-cell leukemia virus type 1 (HTLV-1) to the cytomegalovirus (CMV) enhancer/promoter increased transgene expression 5- to 10-fold and improved cellular immune responses to human immunodeficiency virus type 1 (HIV-1) antigens. In cynomolgus monkeys, DNA vaccines containing the CMV enhancer/promoter with the HTLV-1 R region (CMV/R) induced markedly higher cellular immune responses to HIV-1 Env from clades A, B, and C and to HIV-1 Gag-Pol-Nef compared with the parental DNA vaccines. These data demonstrate that optimization of specific regulatory elements can substantially improve the immunogenicity of DNA vaccines encoding multiple antigens in small animals and in nonhuman primates. This strategy could therefore be explored as a potential method to enhance DNA vaccine immunogenicity in humans. JF - Journal of Virology AU - Barouch, Dan H AU - Yang, Zhi-yong AU - Kong, Wing-pui AU - Korioth-Schmitz, Birgit AU - Sumida, Shawn M AU - Truitt, Diana M AU - Kishko, Michael G AU - Arthur, Janelle C AU - Miura, Ayako AU - Mascola, John R AU - Letvin, Norman L AU - Nabel, Gary J AD - Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215. Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, Maryland 20892-3005 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 8828 EP - 8834 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 14 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Primates KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Immunology Abstracts KW - N 14025:RNA/DNA role in infection & immune response KW - W3 33345:DNA vaccines KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews KW - F 06104:Virus UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17648778?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=A+Human+T-Cell+Leukemia+Virus+Type+1+Regulatory+Element+Enhances+the+Immunogenicity+of+Human+Immunodeficiency+Virus+Type+1+DNA+Vaccines+in+Mice+and+Nonhuman+Primates&rft.au=Barouch%2C+Dan+H%3BYang%2C+Zhi-yong%3BKong%2C+Wing-pui%3BKorioth-Schmitz%2C+Birgit%3BSumida%2C+Shawn+M%3BTruitt%2C+Diana+M%3BKishko%2C+Michael+G%3BArthur%2C+Janelle+C%3BMiura%2C+Ayako%3BMascola%2C+John+R%3BLetvin%2C+Norman+L%3BNabel%2C+Gary+J&rft.aulast=Barouch&rft.aufirst=Dan&rft.date=2005-07-01&rft.volume=79&rft.issue=14&rft.spage=8828&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Letter re: Limited Accuracy of Surrogates of Insulin Resistance during Puberty in Obese and Lean Children at Risk for Altered Glucoregulation AN - 17647606; 6476412 JF - Journal of Clinical Endocrinology and Metabolism AU - Karne, Rajaram J AU - Chen, Hui AU - Sullivan, Gail AU - Quon, Michael J AD - Diabetes Unit, National Center for Complementary and Alternative Medicine, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 4418 EP - 4419 PB - Endocrine Society, 4350 East West Highway Suite 500 Bethesda MD 20814-4426 USA, [mailto:societyservices@endo-society.org], [URL:http://www.endo-society.org/] VL - 90 IS - 7 SN - 0021-972X, 0021-972X KW - Physical Education Index KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17647606?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Endocrinology+and+Metabolism&rft.atitle=Letter+re%3A+Limited+Accuracy+of+Surrogates+of+Insulin+Resistance+during+Puberty+in+Obese+and+Lean+Children+at+Risk+for+Altered+Glucoregulation&rft.au=Karne%2C+Rajaram+J%3BChen%2C+Hui%3BSullivan%2C+Gail%3BQuon%2C+Michael+J&rft.aulast=Karne&rft.aufirst=Rajaram&rft.date=2005-07-01&rft.volume=90&rft.issue=7&rft.spage=4418&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Endocrinology+and+Metabolism&rft.issn=0021972X&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Tyrosine Phosphorylation of the Chlamydial Effector Protein Tarp Is Species Specific and Not Required for Recruitment of Actin AN - 17644268; 6426131 AB - Chlamydiae are obligate intracellular pathogens that efficiently induce their endocytosis by susceptible eukaryotic host cells. Recently, a Chlamydia trachomatis type III secreted effector protein, Tarp, was found to be translocated and tyrosine phosphorylated at the site of entry and associated with the recruitment of actin that coincides with endocytosis. C. trachomatis Tarp possesses up to six direct repeats of approximately 50 amino acids each. The majority of the tyrosine residues are found within this repeat region. Here we have ectopically expressed distinct domains of Tarp in HeLa 229 cells and demonstrated that tyrosine phosphorylation occurs primarily within the repeat region, while recruitment of actin is mediated by the C-terminal domain of the protein. A comparison of other sequenced chlamydial genomes revealed that each contains an ortholog of Tarp, although Chlamydia muridarum, Chlamydophila caviae, and Chlamydophila pneumoniae Tarp lack the large repeat region. Immunofluorescence and immunoblotting using an antiphosphotyrosine antibody show no evidence of phosphotyrosine at the site of entry of C. muridarum, C. caviae, and C. pneumoniae, although each species similarly recruits actin. Ectopic expression of full-length C. trachomatis and C. caviae Tarp confirmed that both recruit actin but only C. trachomatis Tarp is tyrosine phosphorylated. The data indicate that the C-terminal domain of Tarp is essential for actin recruitment and that tyrosine phosphorylation may not be an absolute requirement for actin recruitment. The results further suggest the potential for additional, unknown signal transduction pathways associated specifically with C. trachomatis. JF - Infection and Immunity AU - Clifton, Dawn R AU - Dooley, Cheryl A AU - Grieshaber, Scott S AU - Carabeo, Reynaldo A AU - Fields, Kenneth A AU - Hackstadt, Ted AD - Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana 59840 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 3860 EP - 3868 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 7 SN - 0019-9567, 0019-9567 KW - Tarp protein KW - Microbiology Abstracts B: Bacteriology KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17644268?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Tyrosine+Phosphorylation+of+the+Chlamydial+Effector+Protein+Tarp+Is+Species+Specific+and+Not+Required+for+Recruitment+of+Actin&rft.au=Clifton%2C+Dawn+R%3BDooley%2C+Cheryl+A%3BGrieshaber%2C+Scott+S%3BCarabeo%2C+Reynaldo+A%3BFields%2C+Kenneth+A%3BHackstadt%2C+Ted&rft.aulast=Clifton&rft.aufirst=Dawn&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=3860&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Staphylococcus aureus ClpC Is Required for Stress Resistance, Aconitase Activity, Growth Recovery, and Death AN - 17629651; 6426260 AB - The ability of Staphylococcus aureus to adapt to various conditions of stress is the result of a complex regulatory response. Previously, it has been demonstrated that Clp homologues are important for a variety of stress conditions, and our laboratory has shown that a clpC homologue was highly expressed in the S. aureus strain DSM20231during biofilm formation relative to expression in planktonic cells. Persistence and long-term survival are a hallmark of biofilm-associated staphylococcal infections, as cure frequently fails even in the presence of bactericidal antimicrobials. To determine the role of clpC in this context, we performed metabolic, gene expression, and long-term growth and survival analyses of DSM20231as well as an isogenic clpC allelic-replacement mutant, a sigB mutant, and a clpC sigB double mutant. As expected, the clpC mutant showed increased sensitivity to oxidative and heat stresses. Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the catabolization of acetate during the stationary phase. clpC inactivation abolished post-stationary-phase recovery but also resulted in significantly enhanced stationary-phase survival compared to that of the wild-type strain. These data demonstrate the critical role of the ClpC ATPase in regulating the TCA cycle and implicate ClpC as being important for recovery from the stationary phase and also for entering the death phase. Understanding the stationary- and post-stationary-phase recovery in S. aureus may have important clinical implications, as little is known about the mechanisms of long-term persistence of chronic S. aureus infections associated with formation of biofilms. JF - Journal of Bacteriology AU - Chatterjee, Indranil AU - Becker, Petra AU - Grundmeier, Matthias AU - Bischoff, Markus AU - Somerville, Greg A AU - Peters, Georg AU - Sinha, Bhanu AU - Harraghy, Niamh AU - Proctor, Richard A AU - Herrmann, Mathias AD - Institute of Medical Microbiology and Hygiene, Institutes of Infectious Disease Medicine, University of Saarland, Homburg/Saar, Germany. Institute of Medical Microbiology, University Hospital of Muenster, Muenster, Germany. Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana. Department of Medicine and Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 4488 EP - 4496 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 13 SN - 0021-9193, 0021-9193 KW - ClpC protein KW - citB gene KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - J 02728:Enzymes KW - G 07320:Bacterial genetics KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17629651?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Staphylococcus+aureus+ClpC+Is+Required+for+Stress+Resistance%2C+Aconitase+Activity%2C+Growth+Recovery%2C+and+Death&rft.au=Chatterjee%2C+Indranil%3BBecker%2C+Petra%3BGrundmeier%2C+Matthias%3BBischoff%2C+Markus%3BSomerville%2C+Greg+A%3BPeters%2C+Georg%3BSinha%2C+Bhanu%3BHarraghy%2C+Niamh%3BProctor%2C+Richard+A%3BHerrmann%2C+Mathias&rft.aulast=Chatterjee&rft.aufirst=Indranil&rft.date=2005-07-01&rft.volume=187&rft.issue=13&rft.spage=4488&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Endocrine Perturbation Increases Susceptibility of Mice to Anthrax Lethal Toxin AN - 17629126; 6426176 AB - Bacillus anthracis lethal toxin (LT) causes vascular collapse and high lethality in BALB/cJ mice, intermediate lethality in C57BL/6J mice, and no lethality in DBA/2J mice. We found that adrenalectomized (ADX) mice of all three strains had increased susceptibility to LT. The increased susceptibility of ADX-DBA/2J mice was not accompanied by changes in their macrophage sensitivity or cytokine response to LT. DBA/2J mice showed no change in serum corticosteroid levels in response to LT injection, while BALB/cJ mice showed a fivefold increase in serum corticosterone. However, LT inhibited dexamethasone (DEX)-induced glucocorticoid receptor gene activation to similar extents in all three strains. DEX treatment did not rescue ADX mice from LT-mediated mortality. Surprisingly, oral DEX treatment also sensitized adrenally intact DBA/2J mice to LT lethality at all doses tested and also exacerbated LT-mediated pathogenesis and mortality in BALB/cJ mice. Aldosterone did not protect ADX mice from toxin challenge. These results indicate that susceptibility to anthrax LT in mice depends on a fine but easily perturbed balance of endocrine functions. Thus, the potentially detrimental consequences of steroid therapy for anthrax must be considered in treatment protocols for this disease. JF - Infection and Immunity AU - Moayeri, Mahtab AU - Webster, Jeanette I AU - Wiggins, Jason F AU - Leppla, Stephen H AU - Sternberg, Esther M AD - Microbial Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892. Section on Neuroendocrine Immunology and Behavior, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 4238 EP - 4244 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 7 SN - 0019-9567, 0019-9567 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - G 07320:Bacterial genetics KW - J 02823:In vitro and in vivo effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17629126?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Endocrine+Perturbation+Increases+Susceptibility+of+Mice+to+Anthrax+Lethal+Toxin&rft.au=Moayeri%2C+Mahtab%3BWebster%2C+Jeanette+I%3BWiggins%2C+Jason+F%3BLeppla%2C+Stephen+H%3BSternberg%2C+Esther+M&rft.aulast=Moayeri&rft.aufirst=Mahtab&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=4238&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - DNA Vaccines Expressing Different Forms of Simian Immunodeficiency Virus Antigens Decrease Viremia upon SIVmac251 Challenge AN - 17628955; 6426773 AB - We have tested the efficacy of DNA immunization as a single vaccination modality for rhesus macaques followed by highly pathogenic SIVmac251 challenge. To further improve immunogenicity of the native proteins, we generated expression vectors producing fusion of the proteins Gag and Env to the secreted chemokine MCP3, targeting the viral proteins to the secretory pathway and to a beta -catenin (CATE) peptide, targeting the viral proteins to the intracellular degradation pathway. Macaques immunized with vectors expressing the MCP3-tagged fusion proteins developed stronger antibody responses. Following mucosal challenge with pathogenic SIVmac251, the vaccinated animals showed a statistically significant decrease in viral load (P = 0.010). Interestingly, macaques immunized with a combination of vectors expressing three forms of antigens (native protein and MCP3 and CATE fusion proteins) showed the strongest decrease in viral load (P = 0.0059). Postchallenge enzyme-linked immunospot values for Gag and Env as well as gag-specific T-helper responses correlated with control of viremia. Our data show that the combinations of DNA vaccines producing native and modified forms of antigens elicit more balanced immune responses able to significantly reduce viremia for a long period (8 months) following pathogenic challenge with SIVmac251. JF - Journal of Virology AU - Rosati, Margherita AU - von Gegerfelt, Agneta AU - Roth, Patricia AU - Alicea, Candido AU - Valentin, Antonio AU - Robert-Guroff, Marjorie AU - Venzon, David AU - Montefiori, David C AU - Markham, Phil AU - Felber, Barbara K AU - Pavlakis, George N AD - Human Retrovirus Section. Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland. Immune Biology of Retroviral Infection Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland. Biostatistics and Data Management Section, National Cancer Institute, Bethesda, Maryland. Duke University Medical Center, Durham, North Carolina. Advanced BioScience Laboratories, Inc., Kensington, Maryland Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 8480 EP - 8492 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 13 SN - 0022-538X, 0022-538X KW - Rhesus macaque KW - Rhesus monkey KW - SIV KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Immunology Abstracts; Virology & AIDS Abstracts KW - N 14025:RNA/DNA role in infection & immune response KW - F 06100:Vaccines - active immunity KW - W3 33345:DNA vaccines KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17628955?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=DNA+Vaccines+Expressing+Different+Forms+of+Simian+Immunodeficiency+Virus+Antigens+Decrease+Viremia+upon+SIVmac251+Challenge&rft.au=Rosati%2C+Margherita%3Bvon+Gegerfelt%2C+Agneta%3BRoth%2C+Patricia%3BAlicea%2C+Candido%3BValentin%2C+Antonio%3BRobert-Guroff%2C+Marjorie%3BVenzon%2C+David%3BMontefiori%2C+David+C%3BMarkham%2C+Phil%3BFelber%2C+Barbara+K%3BPavlakis%2C+George+N&rft.aulast=Rosati&rft.aufirst=Margherita&rft.date=2005-07-01&rft.volume=79&rft.issue=13&rft.spage=8480&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Enhanced Breadth of CD4 T-Cell Immunity by DNA Prime and Adenovirus Boost Immunization to Human Immunodeficiency Virus Env and Gag Immunogens AN - 17628922; 6426730 AB - A variety of gene-based vaccination approaches have been used to enhance the immune response to viral pathogens. Among them, the ability to perform heterologous immunization by priming with DNA and boosting with replication-defective adenoviral (ADV) vectors encoding foreign antigens has proven particularly effective in eliciting enhanced cellular and humoral immunity compared to either agent alone. Because adenoviral vector immunization alone can elicit substantial cellular and humoral immune responses in a shorter period of time, we asked whether the immune response induced by the prime-boost immunization was different from adenoviral vaccines with respect to the potency and breadth of T-cell recognition. While DNA/ADV immunization stimulated the CD8 response, it was directed to the same epitopes in Gag and Env immunogens of human immunodeficiency virus as DNA or ADV alone. In contrast, the CD4 response to these immunogens diversified after DNA/ADV immunization compared to each vector alone. These findings suggest that the diversity of the CD4 immune response is increased by DNA/ADV prime-boost vaccination and that these components work synergistically to enhance T-cell epitope recognition. JF - Journal of Virology AU - Wu, Lan AU - Kong, Wing-pui AU - Nabel, Gary J AD - Vaccine Research Center, NIAID, National Institutes of Health, Room 4502, Bldg. 40, MSC-3005, 40 Convent Drive, Bethesda, MD 20892-3005. National Center for AIDS/STD Control and Prevention, China-CDC, 27 Nanwei Road, Xuan Wu District, Beijing, 100050, China Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 8024 EP - 8031 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 13 SN - 0022-538X, 0022-538X KW - HIV KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Immunology Abstracts; Virology & AIDS Abstracts KW - W3 33365:Vaccines (other) KW - N 14025:RNA/DNA role in infection & immune response KW - F 06100:Vaccines - active immunity KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17628922?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Enhanced+Breadth+of+CD4+T-Cell+Immunity+by+DNA+Prime+and+Adenovirus+Boost+Immunization+to+Human+Immunodeficiency+Virus+Env+and+Gag+Immunogens&rft.au=Wu%2C+Lan%3BKong%2C+Wing-pui%3BNabel%2C+Gary+J&rft.aulast=Wu&rft.aufirst=Lan&rft.date=2005-07-01&rft.volume=79&rft.issue=13&rft.spage=8024&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Roles of 3-Deoxy-D-manno-2-Octulosonic Acid Transferase from Moraxella catarrhalis in Lipooligosaccharide Biosynthesis and Virulence AN - 17626915; 6426174 AB - Lipooligosaccharide (LOS), a major outer membrane component of Moraxella catarrhalis, is a possible virulence factor in the pathogenesis of human infections caused by the organism. However, information about the roles of the oligosaccharide chain from LOS in bacterial infection remains limited. Here, a kdtA gene encoding 3-deoxy-D-manno-2-octulosonic acid (Kdo) transferase, which is responsible for adding Kdo residues to the lipid A portion of the LOS, was identified by transposon mutagenesis and construction of an isogenic kdtA mutant in strain O35E. The resulting O35EkdtA mutant produced only lipid A without any core oligosaccharide, and it was viable. Physicochemical and biological analysis revealed that the mutant was susceptible to hydrophobic reagents and a hydrophilic glycopeptide and was sensitive to bactericidal activity of normal human serum. Importantly, the mutant showed decreased toxicity by the Limulus amebocyte lysate assay, reduced adherence to human epithelial cells, and enhanced clearance in lungs and nasopharynx in a mouse aerosol challenge model. These data suggest that the oligosaccharide moiety of the LOS is important for the biological activity of the LOS and the virulence capability of the bacteria in vitro and in vivo. This study may bring new insights into novel vaccines or therapeutic interventions against M. catarrhalis infections. JF - Infection and Immunity AU - Peng, Daxin AU - Choudhury, Biswa P AU - Petralia, Ronald S AU - Carlson, Russell W AU - Gu, Xin-Xing AD - Vaccine Research Section. Section on Neurotransmitter Receptor Biology, National Institute on Deafness and Other Communication Disorders, Rockville, Maryland 20850. Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 4222 EP - 4230 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 7 SN - 0019-9567, 0019-9567 KW - 3-deoxy-D-manno-2-octulosonic acid transferase KW - kdtA gene KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - G 07320:Bacterial genetics KW - J 02728:Enzymes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17626915?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Roles+of+3-Deoxy-D-manno-2-Octulosonic+Acid+Transferase+from+Moraxella+catarrhalis+in+Lipooligosaccharide+Biosynthesis+and+Virulence&rft.au=Peng%2C+Daxin%3BChoudhury%2C+Biswa+P%3BPetralia%2C+Ronald+S%3BCarlson%2C+Russell+W%3BGu%2C+Xin-Xing&rft.aulast=Peng&rft.aufirst=Daxin&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=4222&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Neisseria gonorrhoeae Kills Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 1 (CD66a)-Expressing Human B Cells and Inhibits Antibody Production AN - 17626349; 6426168 AB - Neisseria gonorrhoeae cells (gonococci [GC]), the etiological agents for gonorrhea, can cause repeated infections. During and after gonococcal infection, local and systemic antigonococcal antibody levels are low. These clinical data indicate the possibility that GC may suppress immune responses during infection. Carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1 or CD66a), a receptor for GC opacity (Opa) proteins, was shown to mediate inhibitory signals. In the present study, human B cells were activated by interleukin-2 to express CEACAM1 and then stimulated to secrete antibodies and simultaneously coincubated with Opa super(-) and OpaI GC of strain MS11. Our results show that this OpaI GC has the ability to inhibit antibody production. The interaction of GC and CEACAM1 with human peripheral B cells also results in induction of cell death. The same findings were observed in DT40 B cells. This CEACAM1-promoted cell death pathway does not involve the inhibitory signals or the tyrosine phosphatases SHP-1 and SHP-2 but depends on Bruton's tyrosine kinase in DT40 cells. Our results suggest that Neisseria gonorrhoeae possesses the ability to suppress antibody production by killing CEACAM1-expressing B cells. JF - Infection and Immunity AU - Pantelic, Milica AU - Kim, Young-June AU - Bolland, Silvia AU - Chen, Ines AU - Shively, John AU - Chen, Tie AD - Department of Microbiology and Immunology, Division of Infectious Diseases, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana. Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 12441 Parklawn Drive, Twinbrook II, Room 217, Rockville, Maryland. Public Health Research Institute, 225 Warren Street, Newark, New Jersey. Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 4171 EP - 4179 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 7 SN - 0019-9567, 0019-9567 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17626349?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Neisseria+gonorrhoeae+Kills+Carcinoembryonic+Antigen-Related+Cellular+Adhesion+Molecule+1+%28CD66a%29-Expressing+Human+B+Cells+and+Inhibits+Antibody+Production&rft.au=Pantelic%2C+Milica%3BKim%2C+Young-June%3BBolland%2C+Silvia%3BChen%2C+Ines%3BShively%2C+John%3BChen%2C+Tie&rft.aulast=Pantelic&rft.aufirst=Milica&rft.date=2005-07-01&rft.volume=73&rft.issue=7&rft.spage=4171&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - A new yeast display vector permitting free scFv amino termini can augment ligand binding affinities AN - 17588543; 6481022 AB - Yeast surface display and sorting by flow cytometry are now widely used to direct the evolution of protein binding such as single-chain antibodies or scFvs. The available commercial yeast display vector pYD1 (Invitrogen) displays the protein of interest flanked on the N-terminus by Aga2, the disulfide of which binds the myristylated surface membrane protein Aga1. We have noted that two anti-CD3 epsilon scFvs expressed as fusion proteins suffer a 30- to 100-fold loss of affinity when placed NH sub(2) terminal to either truncated toxins or human serum albumin. In the course of affinity maturing one of these scFv (FN18) using pYD1 we noted that the affinity towards the ectodomain of monkey CD3 epsilon gamma was too low to measure. Consequently we rebuilt pYD1 tethering the scFv off the NH sub(2) terminus of Aga2. This display vector, pYD5, now gave a positive signal displaying FN18 scFv with its ligand, monkey CD3 epsilon gamma . The apparent equilibrium association constant of the higher affinity scFv directed at human CD3 epsilon gamma increased similar to 3-fold when displayed on pYD5 compared with pYD1. These data show that for certain yeast-displayed scFvs a carboxy-tethered scFv can result in increased ligand-scFv equilibrium association constants and thereby extend the low range of affinity maturation measurements. JF - Protein Engineering Design and Selection AU - Wang, Z AU - Mathias, A AU - Stavrou, S AU - Neville, DMJr AD - Section on Biophysical Chemistry, Laboratory of Molecular Biology, National Institute of Mental Health, Building 10, 36 Convent Drive, Bethesda, MD 28092-4034, USA Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 337 EP - 343 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 18 IS - 7 SN - 1741-0126, 1741-0126 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Flow cytometry KW - Antibodies KW - Protein engineering KW - human serum albumin KW - Membrane proteins KW - Fusion protein KW - Toxins KW - Fv KW - N-Terminus KW - W3 33375:Antibodies KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17588543?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Engineering+Design+and+Selection&rft.atitle=A+new+yeast+display+vector+permitting+free+scFv+amino+termini+can+augment+ligand+binding+affinities&rft.au=Wang%2C+Z%3BMathias%2C+A%3BStavrou%2C+S%3BNeville%2C+DMJr&rft.aulast=Wang&rft.aufirst=Z&rft.date=2005-07-01&rft.volume=18&rft.issue=7&rft.spage=337&rft.isbn=&rft.btitle=&rft.title=Protein+Engineering+Design+and+Selection&rft.issn=17410126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Fv; Flow cytometry; Protein engineering; Toxins; Fusion protein; Antibodies; N-Terminus; Membrane proteins; human serum albumin ER - TY - JOUR T1 - Metabolic activation of zebularine, a novel DNA methylation inhibitor, in human bladder carcinoma cells AN - 17568584; 6450595 AB - Zebularine (2(1H)-pyrimidinone riboside, Zeb), a synthetic analogue of cytidine that is a potent inhibitor of cytidine deaminase, has been recently identified as a general inhibitor of DNA methylation. This inhibition of DNA methyltransferase (DNMT) is hypothesized to be mechanism-based and result from formation of a covalent complex between the enzyme and zebularine-substituted DNA. Metabolic activation of Zeb thus requires that it be phosphorylated and incorporated into DNA. We have quantitatively assessed the phosphorylation and DNA incorporation of Zeb in T24 cells using 2-[ super(14)C]-Zeb in conjunction with gradient anion-exchange HPLC and selected enzymatic and spectroscopic analyses. The corresponding 5'-mono-, di-and triphosphates of Zeb were readily formed in a dose-and time-dependent manner. Two additional Zeb-containing metabolites were tentatively identified as diphosphocholine (Zeb-DP-Chol) and diphosphoethanolamine adducts. Intracellular concentrations of Zeb-TP and Zeb-DP-Chol were similar and greatly exceeded those of other metabolites. DNA incorporation occurred but was surpassed by that of RNA by at least seven-fold. Equivalent levels and similar intracellular metabolic patterns were also observed in the Molt-4 (human T-lymphoblasts) and MC38 (murine colon carcinoma) cell lines. For male BALB/c nu/nu mice implanted s.c. with the EJ6 variant of T24 bladder carcinoma and treated i.p. with 500 mg/kg 2-[ super(14)C]-Zeb, the in vivo phosphorylation pattern of Zeb in tumor tissue examined 24 h after drug administration was similar to that observed in vitro. The complex metabolism of Zeb and its limited DNA incorporation suggest that these are the reasons why it is less potent than either 5-azacytidine or 5-aza-2'-deoxycytidine and requires higher doses for equivalent inhibition of DNMT. JF - Biochemical Pharmacology AU - Ben-Kasus, T AU - Ben-Zvi, Z AU - Marquez, V E AU - Kelley, JA AU - Agbaria, R AD - Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel, kelleyj@dc37a.nci.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 121 EP - 133 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 70 IS - 1 SN - 0006-2952, 0006-2952 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - High-performance liquid chromatography KW - 5-aza-2'-deoxycytidine KW - Urinary bladder KW - Carcinoma KW - Tumor cell lines KW - RNA KW - Colon KW - DNA methylation KW - DNA methyltransferase KW - Azacytidine KW - Metabolic activation KW - Cytidine deaminase KW - W3 33374:Antitumor agents KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17568584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+Pharmacology&rft.atitle=Metabolic+activation+of+zebularine%2C+a+novel+DNA+methylation+inhibitor%2C+in+human+bladder+carcinoma+cells&rft.au=Ben-Kasus%2C+T%3BBen-Zvi%2C+Z%3BMarquez%2C+V+E%3BKelley%2C+JA%3BAgbaria%2C+R&rft.aulast=Ben-Kasus&rft.aufirst=T&rft.date=2005-07-01&rft.volume=70&rft.issue=1&rft.spage=121&rft.isbn=&rft.btitle=&rft.title=Biochemical+Pharmacology&rft.issn=00062952&rft_id=info:doi/10.1016%2Fj.bcp.2005.04.010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - DNA methylation; Urinary bladder; Carcinoma; Metabolic activation; Colon; Tumor cell lines; Azacytidine; RNA; DNA methyltransferase; High-performance liquid chromatography; 5-aza-2'-deoxycytidine; Cytidine deaminase DO - http://dx.doi.org/10.1016/j.bcp.2005.04.010 ER - TY - JOUR T1 - Assessing the estrogenicity of environmental chemicals with a stably transfected lactoferrin gene promoter reporter in HeLa cells AN - 17567800; 6448311 AB - Lactoferrin is an important marker protein of the estrogens. In mice, lactoferrin expression is stimulated in the uterus by ligand-bound estrogen receptors (ERs). With this study we aimed to evaluate the effect of different environmental estrogenic chemicals on the mouse lactoferrin gene expression in a cell-based assay. We constructed a reporter that contains the firefly luciferase gene under hormone-inducible control of a 1.1 kbp fragment of the mouse lactoferrin gene promoter. In an attempt to study the promoter regulation in a chromatin context, we stably transfected the construct (pGL3-mLF-Luc) into HeLa cells, and a stable clone (HeLa-mLF-Luc) incorporating the construct was subsequently generated. Transient transfection of HeLa-mLF-Luc cells with ER alpha and ER beta expression plasmids showed that both 17 beta -estradiol (E2) and diethylstilbestrol (DES) at 10 super(-7) M significantly increased luciferase expression via ER alpha and ER beta . Xenoestrogens such as bisphenol A, 4-octylphenol, 4-nonylphenol and the phytoestrogen genistein when used at increasing concentrations (10 super(-8) to 10 super(-5) M) revealed varying magnitudes of activation (1.96-8-fold). The environmental estrogens showed similar magnitudes of luciferase induction when acting through ER alpha and ER beta -mediated pathways. Also, in the absence of ERs, the xenoestrogens could not induce luciferase expression thereby reflecting receptor dependency. Taken together, the results indicate a significant responsiveness of the stably transfected mouse lactoferrin promoter to endogenous estrogen and environmental estrogenic compounds through ERs. This cell-based transcription assay system may be useful in understanding the susceptibility of estrogen target gene expression by these chemicals at the chromatin level. JF - Environmental Toxicology and Pharmacology AU - Ranhotra, H S AU - Teng, C T AD - Gene Regulation Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA, teng@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 42 EP - 47 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 20 IS - 1 SN - 1382-6689, 1382-6689 KW - Toxicology Abstracts KW - Uterus KW - Chromatin KW - Xenoestrogens KW - Bisphenol A KW - Promoters KW - Transfection KW - lactoferrin KW - Phytoestrogens KW - 17^b-Estradiol KW - Diethylstilbestrol KW - Estrogen receptors KW - Genistein KW - X 24240:Miscellaneous UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17567800?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Toxicology+and+Pharmacology&rft.atitle=Assessing+the+estrogenicity+of+environmental+chemicals+with+a+stably+transfected+lactoferrin+gene+promoter+reporter+in+HeLa+cells&rft.au=Ranhotra%2C+H+S%3BTeng%2C+C+T&rft.aulast=Ranhotra&rft.aufirst=H&rft.date=2005-07-01&rft.volume=20&rft.issue=1&rft.spage=42&rft.isbn=&rft.btitle=&rft.title=Environmental+Toxicology+and+Pharmacology&rft.issn=13826689&rft_id=info:doi/10.1016%2Fj.etap.2004.10.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Estrogen receptors; lactoferrin; Promoters; Xenoestrogens; Chromatin; Phytoestrogens; Transfection; 17^b-Estradiol; Uterus; Bisphenol A; Genistein; Diethylstilbestrol DO - http://dx.doi.org/10.1016/j.etap.2004.10.002 ER - TY - JOUR T1 - The Chernobyl Disaster: Cancer following the Accident at the Chernobyl Nuclear Power Plant AN - 17564044; 6427764 JF - Epidemiologic Reviews AU - Hatch, M AU - Ron, E AU - Bouville, A AU - Zablotska, L AU - Howe, G AD - National Cancer Institute, Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Institutes of Health, Department of Health and Human Services, Rockville, MD. Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 56 EP - 66 PB - Johns Hopkins University, School of Hygiene & Public Health, Candler Bldg., Ste. 840 111 Market Place Baltimore MD 21202-6709 USA VL - 27 IS - 1 SN - 0193-936X, 0193-936X KW - Health & Safety Science Abstracts KW - Nuclear power plants KW - Accidents KW - Ukraine, Chernobyl KW - Radioactive fallout KW - Cancer KW - Public health KW - H 11000:Diseases/Injuries/Trauma UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17564044?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiologic+Reviews&rft.atitle=The+Chernobyl+Disaster%3A+Cancer+following+the+Accident+at+the+Chernobyl+Nuclear+Power+Plant&rft.au=Hatch%2C+M%3BRon%2C+E%3BBouville%2C+A%3BZablotska%2C+L%3BHowe%2C+G&rft.aulast=Hatch&rft.aufirst=M&rft.date=2005-07-01&rft.volume=27&rft.issue=1&rft.spage=56&rft.isbn=&rft.btitle=&rft.title=Epidemiologic+Reviews&rft.issn=0193936X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Ukraine, Chernobyl; Cancer; Nuclear power plants; Accidents; Radioactive fallout; Public health ER - TY - JOUR T1 - The Iron-Sulfur Cluster of the Rieske Iron-Sulfur Protein Functions as a Proton-exiting Gate in the Cytochrome bc sub(1) Complex AN - 17558584; 6414528 AB - The destruction of the Rieske iron-sulfur cluster ([2Fe-2S]) in the bc sub(1) complex by hematoporphyrin-promoted photoinactivation resulted in the complex becoming proton-permeable (Miki, T., Yu, L., and Yu, C.-A. (1991) Biochemistry 30, 230-238). To study further the role of this [2Fe-2S] cluster in proton translocation of the bc sub(1) complex, Rhodobacter sphaeroides mutants expressing His-tagged cytochrome bc sub(1) complexes with mutations at the histidine ligands of the [2Fe-2S] cluster were generated and characterized. These mutants lacked the [2Fe-2S] cluster and possessed no bc sub(1) activity. When the mutant complex was co-inlaid in phospholipid vesicles with intact bovine mitochondrial bc sub(1) complex or cytochrome c oxidase, the proton ejection, normally observed in intact reductase or oxidase vesicles during the oxidation of their corresponding substrates, disappeared. This indicated the creation of a proton-leaking channel in the mutant complex, whose [2Fe-2S] cluster was lacking. Insertion of the bc sub(1) complex lacking the head domain of the Rieske iron-sulfur protein, removed by thermolysin digestion, into PL vesicles together with mitochondrial bc sub(1) complex also rendered the vesicles proton-permeable. Addition of the excess purified head domain of the Rieske iron-sulfur protein partially restored the proton-pumping activity. These results indicated that elimination of the [2Fe-2S] cluster in mutant bc sub(1) complexes opened up an otherwise closed proton channel within the bc sub(1) complex. It was speculated that in the normal catalytic cycle of the bc sub(1) complex, the [2Fe-2S] cluster may function as a proton-exiting gate. JF - Journal of Biological Chemistry AU - Gurung, Buddha AU - Yu, Linda AU - Xia, Di AU - Yu, Chang-An AD - Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078 and the Laboratory of Cell Biology Center, NCI, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 24895 EP - 24902 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 26 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Rhodobacter sphaeroides KW - Protons KW - Thermolysin KW - Rieske iron-sulfur protein KW - Mitochondria KW - Cytochrome-c oxidase KW - Digestion KW - reductase KW - Cytochrome bc1 KW - Histidine KW - Oxidation KW - Vesicles KW - Photoinactivation KW - Translocation KW - Mutation KW - Phospholipids KW - J 02728:Enzymes KW - J 02722:Biodegradation, growth, nutrition and leaching KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17558584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=The+Iron-Sulfur+Cluster+of+the+Rieske+Iron-Sulfur+Protein+Functions+as+a+Proton-exiting+Gate+in+the+Cytochrome+bc+sub%281%29+Complex&rft.au=Gurung%2C+Buddha%3BYu%2C+Linda%3BXia%2C+Di%3BYu%2C+Chang-An&rft.aulast=Gurung&rft.aufirst=Buddha&rft.date=2005-07-01&rft.volume=280&rft.issue=26&rft.spage=24895&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Protons; Rieske iron-sulfur protein; Thermolysin; Mitochondria; Cytochrome-c oxidase; Digestion; Cytochrome bc1; reductase; Histidine; Oxidation; Vesicles; Photoinactivation; Mutation; Translocation; Phospholipids; Rhodobacter sphaeroides ER - TY - JOUR T1 - Immunostimulatory neem leaf preparation acts as an adjuvant to enhance the efficacy of poorly immunogenic B16 melanoma surface antigen vaccine AN - 17463969; 6657096 AB - Immunogenecity of the poorly immunogenic B16 melanoma cell surface antigen (B16MelSAg) was enhanced by combining B16MelSAg with NLP in C57BL/6 mice, as evidenced by ELISA and flow cytometry. NLP was as effective as Freund's complete and incomplete adjuvant to generate antibodies recognizing the B16MelSAg. The NLP generated antibody was a gamma globulin with a subtype of IgG1. Splenic lymphocytes from B16MelSAg + NLP treated mice proliferated more rapidly in vitro when stimulated by specific (B16MelSAg) and nonspecific (ConA) stimulators, in comparison to the proliferation detected in B16MelSAg and NLP treated groups. Vaccination of mice with B16MelSAg + NLP more efficiently prevented the growth of B16 melanoma tumor than mice immunized with B16MelSAg or NLP alone. In another experiment, the immune sera (B16MelSAg + NLP) was mixed with B16Mel tumors and injected subcutaneously into syngenic C57BL/6 mice. Tumor burden was less in mice receiving a tumor along with B16MelSAg + NLP generated immune sera than other groups. The B16MelSAg + NLP generated immune sera induced antibody dependent cellular cytotoxicity specifically towards B16Mel tumor cells in vitro. We concluded that NLP might be a potential immune adjuvant for inducing active immunity towards tumor antigens. JF - International Immunopharmacology AU - Baral, Rathindranath AU - Mandal, Ishita AU - Chattopadhyay, Utpala AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute (CNCI), 37, S. P. Mookherjee Road, Kolkata-700026, India, rbaral@hotmail.com Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 1343 EP - 1352 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 5 IS - 7-8 SN - 1567-5769, 1567-5769 KW - mice KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Azadirachta KW - neem KW - Adjuvants KW - Tumor cells KW - Melanoma KW - Flow cytometry KW - Concanavalin A KW - Immunostimulants KW - Enzyme-linked immunosorbent assay KW - Leaves KW - Antigen (tumor-associated) KW - Immunoglobulin G KW - Vaccines KW - F 06150:Immunotherapy KW - W 30965:Miscellaneous, Reviews KW - W3 33360:Adjuvants and carriers UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17463969?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Immunopharmacology&rft.atitle=Immunostimulatory+neem+leaf+preparation+acts+as+an+adjuvant+to+enhance+the+efficacy+of+poorly+immunogenic+B16+melanoma+surface+antigen+vaccine&rft.au=Baral%2C+Rathindranath%3BMandal%2C+Ishita%3BChattopadhyay%2C+Utpala&rft.aulast=Baral&rft.aufirst=Rathindranath&rft.date=2005-07-01&rft.volume=5&rft.issue=7-8&rft.spage=1343&rft.isbn=&rft.btitle=&rft.title=International+Immunopharmacology&rft.issn=15675769&rft_id=info:doi/10.1016%2Fj.intimp.2005.03.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Azadirachta; Melanoma; Adjuvants; Immunostimulants; Antigen (tumor-associated); Tumor cells; Flow cytometry; Leaves; Concanavalin A; Vaccines; neem; Enzyme-linked immunosorbent assay; Immunoglobulin G DO - http://dx.doi.org/10.1016/j.intimp.2005.03.008 ER - TY - JOUR T1 - Intravenous butyrylcholinesterase administration and plasma and brain levels of cocaine and metabolites in rats AN - 17450790; 6644296 AB - Butyrylcholinesterase is a major cocaine-metabolizing enzyme in humans and other primates, catalyzing hydrolysis to ecgonine methylester. Increasing butyrylcholinesterase activity may be a treatment for cocaine addiction. We evaluated the effect of 30-min pretreatment with horse-derived butyrylcholinesterase (5-15,000 U i.v.) or with the selective butyrylcholinesterase inhibitor cymserine (10 mg/kg i.v.) on the metabolism of cocaine (17 mg/kg i.p.) in anesthetized rats. Venous blood samples were collected for two hours after cocaine administration and later assayed for cocaine and metabolites by gas chromatography/mass spectroscopy. Whole brains were collected after the last blood sample and similarly assayed. Butyrylcholinesterase significantly increased plasma and brain ecgonine methylester levels and decreased cocaine plasma half-life from 26.2 min (saline) to 16.4 min (15,000 U). Butyrylcholinesterase had no significant effect on plasma or brain cocaine or benzoylecgonine levels. Cymserine had no effect on any variable. These findings suggest that butyrylcholinesterase treatment may have benefits in enhancing cocaine metabolism and in increasing levels of ecgonine methylester, which may have a protective action against cocaine. JF - European Journal of Pharmacology AU - Carmona, Gilberto N AU - Schindler, Charles W AU - Greig, Nigel H AU - Holloway, Harold W AU - Jufer, Rebecca A AU - Cone, Edward J AU - Gorelick, David A AD - Intramural Research Programs, National Institute on Drug Abuse, Baltimore, MD 21224, USA, dgorelic@intra.nida.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 186 EP - 190 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 517 IS - 3 SN - 0014-2999, 0014-2999 KW - butyrylcholinesterase KW - ecgonine KW - rats KW - CSA Neurosciences Abstracts; Toxicology Abstracts KW - Cocaine KW - Butyrylcholinesterase KW - Ecgonine methylester KW - Cymserine KW - Benzoylecgonine KW - (Rat) KW - Intravenous administration KW - Gas chromatography KW - Brain KW - Enzymes KW - Metabolites KW - Addiction KW - Drug addiction KW - Primates KW - Hydrolysis KW - Metabolism KW - N3 11106:Neurobiology of drug abuse KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17450790?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=European+Journal+of+Pharmacology&rft.atitle=Intravenous+butyrylcholinesterase+administration+and+plasma+and+brain+levels+of+cocaine+and+metabolites+in+rats&rft.au=Carmona%2C+Gilberto+N%3BSchindler%2C+Charles+W%3BGreig%2C+Nigel+H%3BHolloway%2C+Harold+W%3BJufer%2C+Rebecca+A%3BCone%2C+Edward+J%3BGorelick%2C+David+A&rft.aulast=Carmona&rft.aufirst=Gilberto&rft.date=2005-07-01&rft.volume=517&rft.issue=3&rft.spage=186&rft.isbn=&rft.btitle=&rft.title=European+Journal+of+Pharmacology&rft.issn=00142999&rft_id=info:doi/10.1016%2Fj.ejphar.2005.05.013 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Intravenous administration; Gas chromatography; Brain; Enzymes; Metabolites; Addiction; Drug addiction; Cocaine; Hydrolysis; Metabolism; Primates DO - http://dx.doi.org/10.1016/j.ejphar.2005.05.013 ER - TY - JOUR T1 - Androgen Deprivation Therapy for Prostate Cancer AN - 17386194; 6478417 AB - CONTEXT: Prostate cancer is the most common nonskin cancer and second most common cause of cancer mortality in US men. Androgen deprivation therapy (ADT), specifically surgical or medical castration, is the first line of treatment against advanced prostate cancer and is also used as an adjuvant to local treatment of high-risk disease. OBJECTIVE: To review systematically the evidence on the risks and benefits of ADT for prostate cancer as well as clinical management of its adverse effects. Evidence Acquisition We performed MEDLINE searches of English-language literature (1966 to March 2005) using the terms androgen deprivation therapy, hormone treatment, and prostate cancer. We reviewed bibliographies of literature to extract other relevant articles. Studies were selected based on clinical pertinence, with an emphasis on controlled study design. Evidence Synthesis Androgen deprivation therapy is effective for palliation in many patients with advanced prostate cancer and improves outcomes for high-risk patients treated with radiation therapy for localized disease. Although patients with increasing prostate-specific antigen levels after local treatment without metastatic disease frequently undergo ADT, the benefits of this strategy are not clear. Adverse effects of ADT include decreased libido, impotence, hot flashes, osteopenia with increased fracture risk, metabolic alterations, and changes in cognition and mood. CONCLUSIONS: Androgen deprivation therapy has clear roles in the management of advanced prostate cancer and high-risk localized disease. The benefits of ADT in other settings need to be weighed carefully against substantial risks and adverse effects on quality of life. JF - JAMA: Journal of the American Medical Association AU - Sharifi, Nima AU - Gulley, James L AU - Dahut, William L AD - Medical Oncology Clinical Research Unit and Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Md Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 238 EP - 244 PB - American Medical Association, 515 N. State St. Chicago IL 60610 USA VL - 294 IS - 2 SN - 0098-7484, 0098-7484 KW - androgen deprivation therapy KW - prostate KW - Risk Abstracts; Health & Safety Science Abstracts KW - Drugs KW - Cancer KW - Side effects KW - R2 23060:Medical and environmental health KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17386194?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=JAMA%3A+Journal+of+the+American+Medical+Association&rft.atitle=Androgen+Deprivation+Therapy+for+Prostate+Cancer&rft.au=Sharifi%2C+Nima%3BGulley%2C+James+L%3BDahut%2C+William+L&rft.aulast=Sharifi&rft.aufirst=Nima&rft.date=2005-07-01&rft.volume=294&rft.issue=2&rft.spage=238&rft.isbn=&rft.btitle=&rft.title=JAMA%3A+Journal+of+the+American+Medical+Association&rft.issn=00987484&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Drugs; Side effects; Cancer ER - TY - JOUR T1 - Tobacco Smoke Stimulates the Transcription of Amphiregulin in Human Oral Epithelial Cells: Evidence of a Cyclic AMP-Responsive Element Binding Protein-Dependent Mechanism AN - 17379702; 6475527 AB - Activation of epidermal growth factor receptor (EGFR)-mediated signaling has been implicated in the pathogenesis of tobacco smoke-induced cancers. Recently, elevated levels of amphiregulin, a ligand of the EGFR, were found in the oral mucosa of smokers. The main objective of this study was to elucidate the mechanism by which tobacco smoke induces amphiregulin. Treatment of a nontumorigenic human oral epithelial cell line (MSK-Leuk1) with a saline extract of tobacco smoke stimulated amphiregulin (AR) transcription resulting in increased amounts of amphiregulin mRNA and protein. Tobacco smoke stimulated the cyclic AMP (cAMP) arrow right protein kinase A (PKA) pathway leading to increased cAMP-responsive element binding protein-dependent activation of AR transcription. These inductive effects of tobacco smoke were dependent on the aryl hydrocarbon receptor (AhR). In fact, alpha -naphthoflavone, an AhR antagonist, blocked tobacco smoke-mediated induction of binding of cAMP-responsive element binding protein to the AR promoter and thereby suppressed the induction of amphiregulin. Notably, treatment of MSK-Leuk1 cells with tobacco smoke or exogenous amphiregulin stimulated DNA synthesis. An inhibitor of EGFR tyrosine kinase or a neutralizing antibody to amphiregulin abrogated the increase in DNA synthesis mediated by tobacco smoke. Taken together, these findings suggest that tobacco smoke stimulated a signaling pathway comprised of AhR arrow right cAMP arrow right PKA resulting in enhanced AR transcription and increased DNA synthesis. The ability of tobacco smoke to induce amphiregulin and thereby enhance DNA synthesis is likely to contribute to the procarcinogenic effects of tobacco smoke. JF - Cancer Research AU - Du, Baoheng AU - Altorki, Nasser K AU - Kopelovich, Levy AU - Subbaramaiah, Kotha AU - Dannenberg, Andrew J AD - Departments of Medicine and Cardiothoracic Surgery, Weill Medical College of Cornell University, New York, New York and Division of Cancer Prevention, National Cancer Institute, Bethesda, Maryland Y1 - 2005/07/01/ PY - 2005 DA - 2005 Jul 01 SP - 5982 EP - 5988 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 13 SN - 0008-5472, 0008-5472 KW - Biochemistry Abstracts 2: Nucleic Acids; Toxicology Abstracts; Oncogenes & Growth Factors Abstracts KW - Epithelial cells KW - DNA biosynthesis KW - alpha -Naphthoflavone KW - Protein kinase A KW - Regulatory sequences KW - Cyclic AMP KW - Mucosa KW - Transcription KW - Epidermal growth factor receptors KW - Cancer KW - Smoke KW - Promoters KW - Antibodies KW - Protein-tyrosine kinase KW - Tobacco KW - amphiregulin KW - Aryl hydrocarbon receptors KW - Transcription activation KW - Signal transduction KW - Cyclic AMP response element-binding protein KW - B 26240:Other nuclear oncogenes & binding proteins KW - X 24180:Social poisons & drug abuse KW - N 14030:DNA: biosynthesis, repair & replication cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17379702?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=Tobacco+Smoke+Stimulates+the+Transcription+of+Amphiregulin+in+Human+Oral+Epithelial+Cells%3A+Evidence+of+a+Cyclic+AMP-Responsive+Element+Binding+Protein-Dependent+Mechanism&rft.au=Du%2C+Baoheng%3BAltorki%2C+Nasser+K%3BKopelovich%2C+Levy%3BSubbaramaiah%2C+Kotha%3BDannenberg%2C+Andrew+J&rft.aulast=Du&rft.aufirst=Baoheng&rft.date=2005-07-01&rft.volume=65&rft.issue=13&rft.spage=5982&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - alpha -Naphthoflavone; DNA biosynthesis; Epithelial cells; Protein kinase A; Regulatory sequences; Mucosa; Cyclic AMP; Transcription; Epidermal growth factor receptors; Cancer; Smoke; Promoters; Antibodies; Protein-tyrosine kinase; Tobacco; amphiregulin; Aryl hydrocarbon receptors; Transcription activation; Cyclic AMP response element-binding protein; Signal transduction ER - TY - JOUR T1 - Chlamydial IFN- gamma immune evasion is linked to host infection tropism AN - 17379297; 6477384 AB - Chlamydiae are obligate intracellular pathogens that can exhibit a broad host range in infection tropism despite maintaining near genomic identity. Here, we have investigated the molecular basis for this unique host-pathogen relationship. We show that human and murine chlamydial infection tropism is linked to unique host and pathogen genes that have coevolved in response to host immunity. This intimate host-pathogen niche revolves around a restricted repertoire of host species-specific IFN- gamma -mediated effector responses and chlamydial virulence factors capable of inhibiting these effector mechanisms. In human epithelial cells, IFN- gamma induces indoleamine 2,3-dioxygenase expression that inhibits chlamydial growth by depleting host tryptophan pools. Human chlamydial strains, but not the mouse strain, avoid this response by the production of tryptophan synthase that rescues them from tryptophan starvation. Conversely, in murine epithelial cells IFN- gamma induces expression of p47 GTPases, but not indoleamine 2,3-dioxygenase. One of these p47 GTPases (Iigp1) was shown by small interfering RNA silencing experiments to specifically inhibit human strains, but not the mouse strain. Like human strains and their host cells, the murine strain has coevolved with its murine host by producing a large toxin possessing YopT homology, possibly to circumvent host GTPases. Collectively, our findings show chlamydial host infection tropism is determined by IFN- gamma -mediated immunity. JF - Proceedings of the National Academy of Sciences, USA AU - Nelson, David E AU - Virok, Dezso P AU - Wood, Heidi AU - Roshick, Christine AU - Johnson, Raymond M AU - Whitmire, William M AU - Crane, Deborah D AU - Steele-Mortimer, Olivia AU - Kari, Laszlo AU - McClarty, Grant AU - Caldwell, Harlan D AD - Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840 Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 10658 EP - 10663 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 30 SN - 0027-8424, 0027-8424 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - gamma -Interferon KW - Tryptophan KW - Epithelial cells KW - virulence factors KW - Tropism KW - Immunity KW - Pathogens KW - Toxins KW - Tryptophan synthase KW - Tryptophan oxygenase KW - siRNA KW - Chlamydia KW - Guanosinetriphosphatase KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17379297?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Chlamydial+IFN-+gamma+immune+evasion+is+linked+to+host+infection+tropism&rft.au=Nelson%2C+David+E%3BVirok%2C+Dezso+P%3BWood%2C+Heidi%3BRoshick%2C+Christine%3BJohnson%2C+Raymond+M%3BWhitmire%2C+William+M%3BCrane%2C+Deborah+D%3BSteele-Mortimer%2C+Olivia%3BKari%2C+Laszlo%3BMcClarty%2C+Grant%3BCaldwell%2C+Harlan+D&rft.aulast=Nelson&rft.aufirst=David&rft.date=2005-07-01&rft.volume=102&rft.issue=30&rft.spage=10658&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Epithelial cells; Tryptophan; gamma -Interferon; Tryptophan oxygenase; virulence factors; siRNA; Tropism; Pathogens; Immunity; Toxins; Tryptophan synthase; Guanosinetriphosphatase; Chlamydia ER - TY - JOUR T1 - Structure and activity of the atypical serine kinase Rio1 AN - 17359583; 6424609 AB - Rio1 is the founding member of the RIO family of atypical serine kinases that are universally present in all organisms from archaea to mammals. Activity of Rio1 was shown to be absolutely essential in Saccharomyces cerevisiae for the processing of 18S ribosomal RNA, as well as for proper cell cycle progression and chromosome maintenance. We determined high-resolution crystal structures of Archaeoglobus fulgidus Rio1 in the presence and absence of bound nucleotides. Crystallization of Rio1 in the presence of ATP or ADP and manganese ions demonstrated major conformational changes in the active site, compared with the uncomplexed protein. Comparisons of the structure of Rio1 with the previously determined structure of the Rio2 kinase defined the minimal RIO domain and the distinct features of the RIO subfamilies. We report here that Ser108 represents the sole autophosphorylation site of A. fulgidus Rio1 and have therefore established its putative peptide substrate. In addition, we show that a mutant enzyme that cannot be autophosphorylated can still phosphorylate an inactive form of Rio1, as well as a number of typical kinase substrates. JF - FEBS Journal AU - LaRonde-LeBlanc, Nicole AU - Guszczynski, Tad AU - Copeland, Terry AU - Wlodawer, Alexander AD - Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI-Frederick, MD, USA, wlodawer@ncifcrf.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 3698 EP - 3713 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 272 IS - 14 SN - 1742-464X, 1742-464X KW - budding yeast KW - Biochemistry Abstracts 2: Nucleic Acids; ASFA 1: Biological Sciences & Living Resources; Microbiology Abstracts B: Bacteriology KW - Q1 01206:Physiology, biochemistry, biophysics KW - J 02728:Enzymes KW - N 14070:Ribosomes: rRNA, ribosomal proteins, and translation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17359583?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Journal&rft.atitle=Structure+and+activity+of+the+atypical+serine+kinase+Rio1&rft.au=LaRonde-LeBlanc%2C+Nicole%3BGuszczynski%2C+Tad%3BCopeland%2C+Terry%3BWlodawer%2C+Alexander&rft.aulast=LaRonde-LeBlanc&rft.aufirst=Nicole&rft.date=2005-07-01&rft.volume=272&rft.issue=14&rft.spage=3698&rft.isbn=&rft.btitle=&rft.title=FEBS+Journal&rft.issn=1742464X&rft_id=info:doi/10.1111%2Fj.1742-4658.2005.04796.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - SuppNotes - Figures, 6; tables, 1; references, 30. N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1111/j.1742-4658.2005.04796.x ER - TY - JOUR T1 - The Evolution of Flea-borne Transmission in Yersinia pestis AN - 17354634; 6418812 AB - Transmission by fleabite is a recent evolutionary adaptation that distinguishes Yersinia pestis, the agent of plague, from Yersinia pseudotuberculosis and all other enteric bacteria. The very close genetic relationship between Y. pestis and Y. pseudotuberculosis indicates that just a few discrete genetic changes were sufficient to give rise to flea-borne transmission. Y. pestis exhibits a distinct infection phenotype in its flea vector, and a transmissible infection depends on genes that are specifically required in the flea, but not the mammal. Transmission factors identified to date suggest that the rapid evolutionary transition of Y. pestis to flea-borne transmission within the last 1,500 to 20,000 years involved at least three steps: acquisition of the two Y. pestis-specific plasmids by horizontal gene transfer; and recruitment of endogenous chromosomal genes for new functions. Perhaps reflective of the recent adaptation, transmission of Y. pestis by fleas is inefficient, and this likely imposed selective pressure favoring the evolution of increased virulence in this pathogen. JF - Current Issues in Molecular Biology AU - Hinnebusch, B J AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA, jhinnebusch@niaid.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 197 EP - 212 VL - 7 IS - 2 SN - 1467-3037, 1467-3037 KW - Fleas KW - Microbiology Abstracts B: Bacteriology; Entomology Abstracts KW - Adaptations KW - Yersinia pestis KW - Yersinia pseudotuberculosis KW - Vectors KW - Pathogens KW - Plasmids KW - Infection KW - Disease transmission KW - Virulence KW - Genetic relationship KW - Siphonaptera KW - Plague KW - Pseudotuberculosis KW - Evolution KW - J 02870:Invertebrate bacteriology KW - Z 05350:Medical, Veterinary, and Agricultural Entomology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17354634?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Issues+in+Molecular+Biology&rft.atitle=The+Evolution+of+Flea-borne+Transmission+in+Yersinia+pestis&rft.au=Hinnebusch%2C+B+J&rft.aulast=Hinnebusch&rft.aufirst=B&rft.date=2005-07-01&rft.volume=7&rft.issue=2&rft.spage=197&rft.isbn=&rft.btitle=&rft.title=Current+Issues+in+Molecular+Biology&rft.issn=14673037&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Genetic relationship; Virulence; Adaptations; Vectors; Plague; Pathogens; Infection; Plasmids; Pseudotuberculosis; Evolution; Disease transmission; Siphonaptera; Yersinia pseudotuberculosis; Yersinia pestis ER - TY - JOUR T1 - Molecular cloning of a phytase gene (phyM) from Pseudomonas syringae MOK1 AN - 17122061; 6744806 AB - A phytase gene (phy M) was cloned from Pseudomonas syringae MOK1 by two steps of degenerate PCR and inverse PCR. This gene consists of 1,287 nucleotides and encodes a polypeptide of 428 amino acids with a deduced molecular mass of 46,652 kDa. Based on its amino acid sequence, the Phy M shares the active site RHGXRXP and HD sequence motifs, typically characterized by histidine acid phosphatases familly. Each phy M gene fragment encoding mature Phy M with its own signal sequence (pEPSS) and without (pEPSM) was subcloned into the E. coli BL21 (DE3) expression vector, pET22b (+). The enzyme activity in crude extracts of clone pEPSM was 2.514 Umg super(-1) of protein, and about 10-fold higher than that of clone pEPSS. JF - Current Microbiology AU - Cho, Jaiesoon AU - Lee, Changwhan AU - Kang, Seungha AU - Lee, Jaecheon AU - Lee, Honggu AU - Bok, Jinduck AU - Woo, Junghee AU - Moon, Yangsoo AU - Choi, Yunjaie AD - National Institute of Environmental Health Sciences, NIH, DHSS, Research Triangle Park, PO Box 12233,, NC, 27709, USA, cho3@niehs.nih.gov Y1 - 2005/07// PY - 2005 DA - Jul 2005 SP - 11 EP - 15 PB - Springer-Verlag, Life Science Journals, 175 Fifth Ave. New York NY 10010 USA, [mailto:orders@springer-ny.com], [URL:http://www.springer-ny.com/] VL - 51 IS - 1 SN - 0343-8651, 0343-8651 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Expression vectors KW - 6-Phytase KW - Histidine KW - Escherichia coli KW - Polymerase chain reaction KW - Enzymes KW - double prime M gene KW - Pseudomonas syringae KW - Nucleotides KW - Acid phosphatase KW - Amino acid sequence KW - J 02728:Enzymes KW - G 07770:Bacteria KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17122061?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Microbiology&rft.atitle=Molecular+cloning+of+a+phytase+gene+%28phyM%29+from+Pseudomonas+syringae+MOK1&rft.au=Cho%2C+Jaiesoon%3BLee%2C+Changwhan%3BKang%2C+Seungha%3BLee%2C+Jaecheon%3BLee%2C+Honggu%3BBok%2C+Jinduck%3BWoo%2C+Junghee%3BMoon%2C+Yangsoo%3BChoi%2C+Yunjaie&rft.aulast=Cho&rft.aufirst=Jaiesoon&rft.date=2005-07-01&rft.volume=51&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Current+Microbiology&rft.issn=03438651&rft_id=info:doi/10.1007%2Fs00284-005-4482-0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-05-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Expression vectors; 6-Phytase; Histidine; Enzymes; Polymerase chain reaction; double prime M gene; Acid phosphatase; Nucleotides; Amino acid sequence; Escherichia coli; Pseudomonas syringae DO - http://dx.doi.org/10.1007/s00284-005-4482-0 ER - TY - JOUR T1 - Stress-specific signatures: expression profiling of p53 wild-type and -null human cells. AN - 67987669; 15824734 AB - Gene expression responses of human cell lines exposed to a diverse set of stress agents were compared by cDNA microarray hybridization. The B-lymphoblastoid cell line TK6 (p53 wild-type) and its p53-null derivative, NH32, were treated in parallel to facilitate investigation of p53-dependent responses. RNA was extracted 4 h after the beginning of treatment when no notable decrease in cell viability was evident in the cultures. Gene expression signatures were defined that discriminated between four broad general mechanisms of stress agents: Non-DNA-damaging stresses (heat shock, osmotic shock, and 12-O-tetradecanoylphorbol 13-acetate), agents causing mainly oxidative stress (arsenite and hydrogen peroxide), ionizing radiations (neutron and gamma-ray exposures), and other DNA-damaging agents (ultraviolet radiation, methyl methanesulfonate, adriamycin, camptothecin, and cis-Platinum(II)diammine dichloride (cisplatin)). Within this data set, non-DNA-damaging stresses could be discriminated from all DNA-damaging stresses, and profiles for individual agents were also defined. While DNA-damaging stresses showed a strong p53-dependent element in their responses, no discernible p53-dependent responses were triggered by the non-DNA-damaging stresses. A set of 16 genes did exhibit a robust p53-dependent pattern of induction in response to all nine DNA-damaging agents, however. JF - Oncogene AU - Amundson, Sally A AU - Do, Khanh T AU - Vinikoor, Lisa AU - Koch-Paiz, Christine A AU - Bittner, Michael L AU - Trent, Jeffrey M AU - Meltzer, Paul AU - Fornace, Albert J AD - Gene Response Section, Center for Cancer Research, NCI, Bethesda, MD 20892, USA. Y1 - 2005/06/30/ PY - 2005 DA - 2005 Jun 30 SP - 4572 EP - 4579 VL - 24 IS - 28 SN - 0950-9232, 0950-9232 KW - Mesylates KW - 0 KW - Oxidants KW - Tumor Suppressor Protein p53 KW - methanesulfonic acid KW - 12EH9M7279 KW - Doxorubicin KW - 80168379AG KW - Cisplatin KW - Q20Q21Q62J KW - Index Medicus KW - Space life sciences KW - NASA Discipline Radiation Health KW - Non-NASA Center KW - B-Lymphocytes -- drug effects KW - Ultraviolet Rays KW - Osmotic Pressure KW - Oligonucleotide Array Sequence Analysis KW - Gamma Rays KW - Cells, Cultured KW - Humans KW - Cisplatin -- toxicity KW - B-Lymphocytes -- radiation effects KW - Oxidants -- toxicity KW - Doxorubicin -- toxicity KW - Mesylates -- toxicity KW - DNA Damage -- genetics KW - B-Lymphocytes -- physiology KW - Heat-Shock Response -- genetics KW - Gene Expression Profiling KW - Tumor Suppressor Protein p53 -- radiation effects KW - Tumor Suppressor Protein p53 -- drug effects KW - Tumor Suppressor Protein p53 -- genetics KW - Stress, Physiological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67987669?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene&rft.atitle=Stress-specific+signatures%3A+expression+profiling+of+p53+wild-type+and+-null+human+cells.&rft.au=Amundson%2C+Sally+A%3BDo%2C+Khanh+T%3BVinikoor%2C+Lisa%3BKoch-Paiz%2C+Christine+A%3BBittner%2C+Michael+L%3BTrent%2C+Jeffrey+M%3BMeltzer%2C+Paul%3BFornace%2C+Albert+J&rft.aulast=Amundson&rft.aufirst=Sally&rft.date=2005-06-30&rft.volume=24&rft.issue=28&rft.spage=4572&rft.isbn=&rft.btitle=&rft.title=Oncogene&rft.issn=09509232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-06-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Clinical practice. Lung cancer screening. AN - 67986315; 15987920 JF - The New England journal of medicine AU - Mulshine, James L AU - Sullivan, Daniel C AD - Intervention Section, Cell and Cancer Biology Branch, Lung Cancer and Aerodigestive Chemoprevention Faculty, Center for Cancer Research, National Cancer Institute, Bethesda, Md 20892, USA. mulshinj@mail.nih.gov Y1 - 2005/06/30/ PY - 2005 DA - 2005 Jun 30 SP - 2714 EP - 2720 VL - 352 IS - 26 KW - Abridged Index Medicus KW - Index Medicus KW - Carcinoma, Small Cell -- pathology KW - Randomized Controlled Trials as Topic KW - Carcinoma, Non-Small-Cell Lung -- diagnostic imaging KW - Humans KW - Practice Guidelines as Topic KW - Carcinoma, Small Cell -- diagnostic imaging KW - Middle Aged KW - Female KW - Tomography, X-Ray Computed KW - Smoking -- adverse effects KW - Lung Neoplasms -- chemically induced KW - Lung Neoplasms -- diagnostic imaging KW - Lung Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67986315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Clinical+practice.+Lung+cancer+screening.&rft.au=Mulshine%2C+James+L%3BSullivan%2C+Daniel+C&rft.aulast=Mulshine&rft.aufirst=James&rft.date=2005-06-30&rft.volume=352&rft.issue=26&rft.spage=2714&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=1533-4406&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-06-30 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: N Engl J Med. 2005 Nov 17;353(20):2194-5; author reply 2194-5 [16291993] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Understanding Barriers to Healthcare among Latinos: The Effects of Ethnicity, Culture Change and Discrimination T2 - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AN - 39731602; 3958608 JF - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AU - Perez, Debra Joy Y1 - 2005/06/26/ PY - 2005 DA - 2005 Jun 26 KW - Ethnic groups KW - Barriers KW - Discrimination KW - Health care KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39731602?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.atitle=Understanding+Barriers+to+Healthcare+among+Latinos%3A+The+Effects+of+Ethnicity%2C+Culture+Change+and+Discrimination&rft.au=Perez%2C+Debra+Joy&rft.aulast=Perez&rft.aufirst=Debra&rft.date=2005-06-26&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.academyhealth.org/2005/abstracts.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - "It's Broke. Can we Fix it?": A Community Forum Theatre Dialogue on Disparities in Access to Health Care T2 - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AN - 39718327; 3959400 JF - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AU - Sullivan, John AU - Stanford, Mathew Y1 - 2005/06/26/ PY - 2005 DA - 2005 Jun 26 KW - Health care KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39718327?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.atitle=%22It%27s+Broke.+Can+we+Fix+it%3F%22%3A+A+Community+Forum+Theatre+Dialogue+on+Disparities+in+Access+to+Health+Care&rft.au=Sullivan%2C+John%3BStanford%2C+Mathew&rft.aulast=Sullivan&rft.aufirst=John&rft.date=2005-06-26&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.academyhealth.org/2005/abstracts.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Prevalence of Discrimination among Latinos in the US: Cultural and SES Correlates T2 - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AN - 39677552; 3958684 JF - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AU - Perez, Debra Joy AU - Alegria, Margarita AU - Fortuna, Lisa Y1 - 2005/06/26/ PY - 2005 DA - 2005 Jun 26 KW - Ethnic groups KW - Discrimination KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39677552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.atitle=The+Prevalence+of+Discrimination+among+Latinos+in+the+US%3A+Cultural+and+SES+Correlates&rft.au=Perez%2C+Debra+Joy%3BAlegria%2C+Margarita%3BFortuna%2C+Lisa&rft.aulast=Perez&rft.aufirst=Debra&rft.date=2005-06-26&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.academyhealth.org/2005/abstracts.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Italian Mental Health Care Reform and Psychiatric Deinstitutionalization in Anthropological, Political, and Historical Perspective T2 - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AN - 39650178; 3958811 JF - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AU - Bergstresser, Sara Y1 - 2005/06/26/ PY - 2005 DA - 2005 Jun 26 KW - Politics KW - Social aspects KW - Health care KW - Historical account KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39650178?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.atitle=Italian+Mental+Health+Care+Reform+and+Psychiatric+Deinstitutionalization+in+Anthropological%2C+Political%2C+and+Historical+Perspective&rft.au=Bergstresser%2C+Sara&rft.aulast=Bergstresser&rft.aufirst=Sara&rft.date=2005-06-26&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.academyhealth.org/2005/abstracts.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Role of Sociocultural and Discrimination Factors in Predicting Support for Health Policy among US Latinos T2 - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AN - 39586214; 3959382 JF - 2005 Annual Research Meeting of the AcademyHealth (ARM 2005) AU - Perez, Debra Joy Y1 - 2005/06/26/ PY - 2005 DA - 2005 Jun 26 KW - Ethnic groups KW - Policies KW - Discrimination KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39586214?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.atitle=The+Role+of+Sociocultural+and+Discrimination+Factors+in+Predicting+Support+for+Health+Policy+among+US+Latinos&rft.au=Perez%2C+Debra+Joy&rft.aulast=Perez&rft.aufirst=Debra&rft.date=2005-06-26&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Annual+Research+Meeting+of+the+AcademyHealth+%28ARM+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.academyhealth.org/2005/abstracts.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Semantic Model of NCI Thesaurus: Representing Genes and Alleles T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 40039968; 3956071 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - de Coronado, Sherri AU - Fragoso, Gilberto AU - Hartel, Francis AU - Lyman, Dan AU - Srivastava, Ranjana Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Models KW - Allelles KW - Thesaurus KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40039968?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=Semantic+Model+of+NCI+Thesaurus%3A+Representing+Genes+and+Alleles&rft.au=de+Coronado%2C+Sherri%3BFragoso%2C+Gilberto%3BHartel%2C+Francis%3BLyman%2C+Dan%3BSrivastava%2C+Ranjana&rft.aulast=de+Coronado&rft.aufirst=Sherri&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - CDTree: A Tool to Analyze and Annotate Protein Subfamily Hierarchies T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 40035141; 3956518 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - Liu, Chunlei Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Databases KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40035141?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=CDTree%3A+A+Tool+to+Analyze+and+Annotate+Protein+Subfamily+Hierarchies&rft.au=Liu%2C+Chunlei&rft.aulast=Liu&rft.aufirst=Chunlei&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Analysis of Genomic Alterations in Tumor Samples with Affymetrix 100K SNP chips T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 39989871; 3956361 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - Kotliarov, Yuri AU - Christopher, Neil AU - Steed, Mary AU - Walling, Jennifer AU - Center, Angela AU - Bogler, Oliver AU - Mikkelsen, Tom AU - Zenklusen, Jean AU - Fine, Howard Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Single-nucleotide polymorphism KW - genomics KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39989871?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=Analysis+of+Genomic+Alterations+in+Tumor+Samples+with+Affymetrix+100K+SNP+chips&rft.au=Kotliarov%2C+Yuri%3BChristopher%2C+Neil%3BSteed%2C+Mary%3BWalling%2C+Jennifer%3BCenter%2C+Angela%3BBogler%2C+Oliver%3BMikkelsen%2C+Tom%3BZenklusen%2C+Jean%3BFine%2C+Howard&rft.aulast=Kotliarov&rft.aufirst=Yuri&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - caArray Data Management and Analysis Tools at the National Cancer Institute (NCI) Center for Bioinformatics T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 39970274; 3956519 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - Heiskanen, Mervi AU - Gustafson, Scott AU - Bian, Xiaopeng AU - Lorenz, Juergen AU - Muju, Sumeet AU - Tran, Phu AU - Moy, John AU - Madhavan, Subha AU - Dubman, Sue AU - Buetow, Ken AU - Zhou, Zim AU - Neuberger, Beth AU - Chen, Hangijiong AU - Bauer, David AU - Shinohara, Andrew AU - Wu, Ye AU - Addepalli, Durga AU - Settnek, Sharon AU - Mejorada, Johnpaul Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Bioinformatics KW - Data processing KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39970274?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=caArray+Data+Management+and+Analysis+Tools+at+the+National+Cancer+Institute+%28NCI%29+Center+for+Bioinformatics&rft.au=Heiskanen%2C+Mervi%3BGustafson%2C+Scott%3BBian%2C+Xiaopeng%3BLorenz%2C+Juergen%3BMuju%2C+Sumeet%3BTran%2C+Phu%3BMoy%2C+John%3BMadhavan%2C+Subha%3BDubman%2C+Sue%3BBuetow%2C+Ken%3BZhou%2C+Zim%3BNeuberger%2C+Beth%3BChen%2C+Hangijiong%3BBauer%2C+David%3BShinohara%2C+Andrew%3BWu%2C+Ye%3BAddepalli%2C+Durga%3BSettnek%2C+Sharon%3BMejorada%2C+Johnpaul&rft.aulast=Heiskanen&rft.aufirst=Mervi&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Rembrandt: Synergistic Leverage of High-Throughput Molecular Profiling, Clinical Data and Bioinformatics to Improve Patient Outcome T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 39962766; 3956517 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - Madhavan, Subha AU - Himanso, Sahni AU - Xiao, Nick AU - Kotliarov, Yuri AU - Luo, James AU - Heiskanen, Mervi AU - Dubman, Sue AU - Zenklusen, Jean Claude AU - Fine, Howard AU - Buetow, Kenneth Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Bioinformatics KW - Profiling KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39962766?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=Rembrandt%3A+Synergistic+Leverage+of+High-Throughput+Molecular+Profiling%2C+Clinical+Data+and+Bioinformatics+to+Improve+Patient+Outcome&rft.au=Madhavan%2C+Subha%3BHimanso%2C+Sahni%3BXiao%2C+Nick%3BKotliarov%2C+Yuri%3BLuo%2C+James%3BHeiskanen%2C+Mervi%3BDubman%2C+Sue%3BZenklusen%2C+Jean+Claude%3BFine%2C+Howard%3BBuetow%2C+Kenneth&rft.aulast=Madhavan&rft.aufirst=Subha&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Phylogeny of Tumor Progression T2 - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AN - 39935103; 3956152 JF - 13th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB 2005) AU - Bilke, Sven AU - Chen, Qingrong AU - Whiteford, Craig AU - Khan, Javed Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Phylogeny KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39935103?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.atitle=Phylogeny+of+Tumor+Progression&rft.au=Bilke%2C+Sven%3BChen%2C+Qingrong%3BWhiteford%2C+Craig%3BKhan%2C+Javed&rft.aulast=Bilke&rft.aufirst=Sven&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=13th+Annual+International+Conference+on+Intelligent+Systems+for+Molecular+Biology+%28ISMB+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.iscb.org/ismb2005/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Chaperone-Procollagen Interactions Differ with Mutation Location in Osteogenesis Imperfecta T2 - 2nd Joint Meeting of the European Calcified Tissue Society and the International Bone and Mineral Society (ECTS-IBMS 2005) AN - 39632102; 3945410 JF - 2nd Joint Meeting of the European Calcified Tissue Society and the International Bone and Mineral Society (ECTS-IBMS 2005) AU - Ashok, A AU - Barnes, A M AU - Marini, J C Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Osteogenesis imperfecta KW - Mutation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39632102?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2nd+Joint+Meeting+of+the+European+Calcified+Tissue+Society+and+the+International+Bone+and+Mineral+Society+%28ECTS-IBMS+2005%29&rft.atitle=Chaperone-Procollagen+Interactions+Differ+with+Mutation+Location+in+Osteogenesis+Imperfecta&rft.au=Ashok%2C+A%3BBarnes%2C+A+M%3BMarini%2C+J+C&rft.aulast=Ashok&rft.aufirst=A&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2nd+Joint+Meeting+of+the+European+Calcified+Tissue+Society+and+the+International+Bone+and+Mineral+Society+%28ECTS-IBMS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.ects-ibms-2005.org/post/index.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Homozygosity for a Dominant-Negative Type I Collagen Mutation Attenuates the Type IV OI Phenotype of the Heterozygous BRTl Mouse: Insight into Disease Mechanism T2 - 2nd Joint Meeting of the European Calcified Tissue Society and the International Bone and Mineral Society (ECTS-IBMS 2005) AN - 39546913; 3945197 JF - 2nd Joint Meeting of the European Calcified Tissue Society and the International Bone and Mineral Society (ECTS-IBMS 2005) AU - Uveges, T E AU - Bergwitz, C AU - Kozloff, K M AU - Forlino, A AU - Kuznetsova, N V AU - Gronowitz, G AU - Goldstein, S A AU - Leikin, S AU - Marini, J C Y1 - 2005/06/25/ PY - 2005 DA - 2005 Jun 25 KW - Collagen (type I) KW - Phenotypes KW - Mutation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39546913?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2nd+Joint+Meeting+of+the+European+Calcified+Tissue+Society+and+the+International+Bone+and+Mineral+Society+%28ECTS-IBMS+2005%29&rft.atitle=Homozygosity+for+a+Dominant-Negative+Type+I+Collagen+Mutation+Attenuates+the+Type+IV+OI+Phenotype+of+the+Heterozygous+BRTl+Mouse%3A+Insight+into+Disease+Mechanism&rft.au=Uveges%2C+T+E%3BBergwitz%2C+C%3BKozloff%2C+K+M%3BForlino%2C+A%3BKuznetsova%2C+N+V%3BGronowitz%2C+G%3BGoldstein%2C+S+A%3BLeikin%2C+S%3BMarini%2C+J+C&rft.aulast=Uveges&rft.aufirst=T&rft.date=2005-06-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2nd+Joint+Meeting+of+the+European+Calcified+Tissue+Society+and+the+International+Bone+and+Mineral+Society+%28ECTS-IBMS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.ects-ibms-2005.org/post/index.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Two-step mechanism that determines the donor binding specificity of human UDP-N-acetylhexosaminyltransferase. AN - 67944452; 15831490 AB - In its x-ray crystal structures, alpha-1,4-N-acteylhexosaminyltransferase (exostosin-like protein 2 (EXTL2)) forms no direct interaction with the N-acetyl group of the UDP-N-acetylhexosamine. Mutation of the residues that interact with the hydroxyl groups of the donor not only failed to abrogate donor binding but in fact increased binding affinity. Isothermal titration calorimetry is now used to examine the binding nature of various UDP-sugars in H2O and D2O solutions. UDP-N-acetylhexosamines bind to EXTL2 with a high affinity in both solutions, resulting in a relatively large increase of entropy, whereas the weak binding of UDP-galactose and -glucose, which occurred only in D2O solution, only slightly increased entropy. Thus, specific donor binding appears to undergo two distinct steps, beginning with the N-acetyl group expelling water from the donor. enzyme complex into the bulk solvent followed by positioning of the donor into the binding site for the subsequent interactions with the enzyme. JF - The Journal of biological chemistry AU - Sobhany, Mack AU - Dong, Jian AU - Negishi, Masahiko AD - Pharmacogenetic Section, Laboratory of Reproductive and Developmental Toxicology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/06/24/ PY - 2005 DA - 2005 Jun 24 SP - 23441 EP - 23445 VL - 280 IS - 25 SN - 0021-9258, 0021-9258 KW - DNA Primers KW - 0 KW - Membrane Proteins KW - EXTL2 protein, human KW - EC 2.4.1.- KW - N-Acetylglucosaminyltransferases KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Kinetics KW - Humans KW - Calorimetry KW - Substrate Specificity KW - Protein Binding KW - N-Acetylglucosaminyltransferases -- metabolism KW - Membrane Proteins -- metabolism KW - N-Acetylglucosaminyltransferases -- genetics KW - Membrane Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67944452?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Two-step+mechanism+that+determines+the+donor+binding+specificity+of+human+UDP-N-acetylhexosaminyltransferase.&rft.au=Sobhany%2C+Mack%3BDong%2C+Jian%3BNegishi%2C+Masahiko&rft.aulast=Sobhany&rft.aufirst=Mack&rft.date=2005-06-24&rft.volume=280&rft.issue=25&rft.spage=23441&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - An Adaptable Assessment Generation System for Clinical Trials Complementing Human Research Information System T2 - 18th IEEE Symposium on Computer-Based Medical Systems (CBMS 2005) AN - 39637247; 3948717 JF - 18th IEEE Symposium on Computer-Based Medical Systems (CBMS 2005) AU - Vahabzadeh, M AU - Lin, J.-L. AU - Mezghanni, M AU - Gupman, A AU - Schmittner, J AU - Preston, K Y1 - 2005/06/23/ PY - 2005 DA - 2005 Jun 23 KW - Clinical trials KW - Information systems KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39637247?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=18th+IEEE+Symposium+on+Computer-Based+Medical+Systems+%28CBMS+2005%29&rft.atitle=An+Adaptable+Assessment+Generation+System+for+Clinical+Trials+Complementing+Human+Research+Information+System&rft.au=Vahabzadeh%2C+M%3BLin%2C+J.-L.%3BMezghanni%2C+M%3BGupman%2C+A%3BSchmittner%2C+J%3BPreston%2C+K&rft.aulast=Vahabzadeh&rft.aufirst=M&rft.date=2005-06-23&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=18th+IEEE+Symposium+on+Computer-Based+Medical+Systems+%28CBMS+2005%29&rft.issn=&rft_id=info:doi/ L2 - https://www.cs.tcd.ie/research_groups/mlg/CBMS2005/preliminary_programme.ht m LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Radio Frequency Ablation Registration, Segmentation, and Fusion Tool T2 - 18th IEEE Symposium on Computer-Based Medical Systems (CBMS 2005) AN - 39609093; 3948684 JF - 18th IEEE Symposium on Computer-Based Medical Systems (CBMS 2005) AU - McCreedy, E AU - Cheng, R AU - Hemler, P AU - Viswanathan, A AU - Wood, B AU - McAuliffe, M Y1 - 2005/06/23/ PY - 2005 DA - 2005 Jun 23 KW - Ablation KW - Radio KW - Segmentation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39609093?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=18th+IEEE+Symposium+on+Computer-Based+Medical+Systems+%28CBMS+2005%29&rft.atitle=Radio+Frequency+Ablation+Registration%2C+Segmentation%2C+and+Fusion+Tool&rft.au=McCreedy%2C+E%3BCheng%2C+R%3BHemler%2C+P%3BViswanathan%2C+A%3BWood%2C+B%3BMcAuliffe%2C+M&rft.aulast=McCreedy&rft.aufirst=E&rft.date=2005-06-23&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=18th+IEEE+Symposium+on+Computer-Based+Medical+Systems+%28CBMS+2005%29&rft.issn=&rft_id=info:doi/ L2 - https://www.cs.tcd.ie/research_groups/mlg/CBMS2005/preliminary_programme.ht m LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Abnormal display of PfEMP-1 on erythrocytes carrying haemoglobin C may protect against malaria AN - 17618641; 6261374 AB - Haemoglobin C, which carries a glutamate-to-lysine mutation in the beta - globin chain, protects West African children against Plasmodium falciparum malaria. Mechanisms of protection are not established for the heterozygous (haemoglobin AC) or homozygous (haemoglobin CC) states. Here we report a marked effect of haemoglobin C on the cell-surface properties of P. falciparum- infected erythrocytes involved in pathogenesis. Relative to parasite-infected normal erythrocytes (haemoglobin AA), parasitized AC and CC erythrocytes show reduced adhesion to endothelial monolayers expressing CD36 and intercellular adhesion molecule-1 (ICAM-1). They also show impaired rosetting interactions with non-parasitized erythrocytes, and reduced agglutination in the presence of pooled sera from malaria-immune adults. Abnormal cell-surface display of the main variable cytoadherence ligand, PfEMP-1 (P. falciparum erythrocyte membrane protein-1), correlates with these findings. The abnormalities in PfEMP- 1 display are associated with markers of erythrocyte senescence, and are greater in CC than in AC erythrocytes. Haemoglobin C might protect against malaria by reducing PfEMP-1-mediated adherence of parasitized erythrocytes, thereby mitigating the effects of their sequestration in the microvasculature. JF - Nature AU - Fairhurst, Rick M AU - Baruch, Dror I AU - Brittain, Nathaniel J AU - Ostera, Graciela R AU - Wallach, John S AU - Hoang, Holly L AU - Hayton, Karen AU - Guindo, Aldiouma AU - Makobongo, Morris O AU - Schwartz, Owen M AU - Tounkara, Anatole AU - Doumbo, Ogobara K AU - Diallo, Dapa A AU - Fujioka, Hisashi AU - Ho, May AU - Wellems, Thomas E AD - Laboratory of Malaria and Vector Research,, twellems@niaid.nih.gov Y1 - 2005/06/23/ PY - 2005 DA - 2005 Jun 23 SP - 1117 EP - 1121 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 435 IS - 7045 SN - 0028-0836, 0028-0836 KW - EMP-1 protein KW - Haemoglobin C KW - Mosquitoes KW - Genetics Abstracts; ASFA 1: Biological Sciences & Living Resources; ASFA 3: Aquatic Pollution & Environmental Quality; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Freshwater KW - Q5 01524:Public health, medicines, dangerous organisms KW - K 03090:Protozoa: human KW - G 07432:Proteins KW - Q1 01484:Species interactions: parasites and diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17618641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Abnormal+display+of+PfEMP-1+on+erythrocytes+carrying+haemoglobin+C+may+protect+against+malaria&rft.au=Fairhurst%2C+Rick+M%3BBaruch%2C+Dror+I%3BBrittain%2C+Nathaniel+J%3BOstera%2C+Graciela+R%3BWallach%2C+John+S%3BHoang%2C+Holly+L%3BHayton%2C+Karen%3BGuindo%2C+Aldiouma%3BMakobongo%2C+Morris+O%3BSchwartz%2C+Owen+M%3BTounkara%2C+Anatole%3BDoumbo%2C+Ogobara+K%3BDiallo%2C+Dapa+A%3BFujioka%2C+Hisashi%3BHo%2C+May%3BWellems%2C+Thomas+E&rft.aulast=Fairhurst&rft.aufirst=Rick&rft.date=2005-06-23&rft.volume=435&rft.issue=7045&rft.spage=1117&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=00280836&rft_id=info:doi/10.1038%2Fnature03631 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Freshwater DO - http://dx.doi.org/10.1038/nature03631 ER - TY - CPAPER T1 - Dose-Dependent Effects of Pro-Inflammatory Cytokines IL-1 and TNF on Tissue-Engineered Cartilage T2 - 2005 Summer Bioengineering Conference AN - 40076539; 3955705 JF - 2005 Summer Bioengineering Conference AU - Byers, Benjamin A AU - Mauck, Robert L AU - Tuan, Rocky S Y1 - 2005/06/22/ PY - 2005 DA - 2005 Jun 22 KW - Cartilage KW - Tissue engineering KW - Tumor necrosis factor KW - Interleukin 1 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40076539?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Summer+Bioengineering+Conference&rft.atitle=Dose-Dependent+Effects+of+Pro-Inflammatory+Cytokines+IL-1+and+TNF+on+Tissue-Engineered+Cartilage&rft.au=Byers%2C+Benjamin+A%3BMauck%2C+Robert+L%3BTuan%2C+Rocky+S&rft.aulast=Byers&rft.aufirst=Benjamin&rft.date=2005-06-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Summer+Bioengineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://divisions.asme.org/bed/events/pdffiles/SBC2005_Tech_Prog.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Enhanced Chondrogenesis and Development of Mechanical Properties of Human Mesenchymal Stem Cells Seeded in a Self-Assembling Peptide Hydrogel T2 - 2005 Summer Bioengineering Conference AN - 39964464; 3955718 JF - 2005 Summer Bioengineering Conference AU - Mauck, Robert L AU - Helm, Jeannine M AU - Tuan, Rocky S Y1 - 2005/06/22/ PY - 2005 DA - 2005 Jun 22 KW - Stem cells KW - hydrogels KW - Mechanical properties KW - Chondrogenesis KW - Peptides KW - Mesenchyme KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39964464?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Summer+Bioengineering+Conference&rft.atitle=Enhanced+Chondrogenesis+and+Development+of+Mechanical+Properties+of+Human+Mesenchymal+Stem+Cells+Seeded+in+a+Self-Assembling+Peptide+Hydrogel&rft.au=Mauck%2C+Robert+L%3BHelm%2C+Jeannine+M%3BTuan%2C+Rocky+S&rft.aulast=Mauck&rft.aufirst=Robert&rft.date=2005-06-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Summer+Bioengineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://divisions.asme.org/bed/events/pdffiles/SBC2005_Tech_Prog.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - On the Ability of Mesenchymal Stem Cells to form Functional Cartilaginous Tissues in Three Dimensional Agarose Culture T2 - 2005 Summer Bioengineering Conference AN - 39958867; 3955491 JF - 2005 Summer Bioengineering Conference AU - Mauck, Robert L AU - Yuan, Xiaoning AU - Tuan, Rocky S Y1 - 2005/06/22/ PY - 2005 DA - 2005 Jun 22 KW - Stem cells KW - Agarose KW - Cell culture KW - Mesenchyme KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39958867?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2005+Summer+Bioengineering+Conference&rft.atitle=On+the+Ability+of+Mesenchymal+Stem+Cells+to+form+Functional+Cartilaginous+Tissues+in+Three+Dimensional+Agarose+Culture&rft.au=Mauck%2C+Robert+L%3BYuan%2C+Xiaoning%3BTuan%2C+Rocky+S&rft.aulast=Mauck&rft.aufirst=Robert&rft.date=2005-06-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2005+Summer+Bioengineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://divisions.asme.org/bed/events/pdffiles/SBC2005_Tech_Prog.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - About the First Public Private Partnership for Reference Image Databases, as Announced at the RSNA 2004 T2 - 19th International Congress and Exhibition on Computer Assisted Radiology and Surgery (CARS 2005) AN - 39724466; 3954373 JF - 19th International Congress and Exhibition on Computer Assisted Radiology and Surgery (CARS 2005) AU - Clarke, L P Y1 - 2005/06/22/ PY - 2005 DA - 2005 Jun 22 KW - Databases KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39724466?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=19th+International+Congress+and+Exhibition+on+Computer+Assisted+Radiology+and+Surgery+%28CARS+2005%29&rft.atitle=About+the+First+Public+Private+Partnership+for+Reference+Image+Databases%2C+as+Announced+at+the+RSNA+2004&rft.au=Clarke%2C+L+P&rft.aulast=Clarke&rft.aufirst=L&rft.date=2005-06-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=19th+International+Congress+and+Exhibition+on+Computer+Assisted+Radiology+and+Surgery+%28CARS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.cars-int.org/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Alcohol and the cardiovascular system: research challenges and opportunities. AN - 67949186; 15963387 AB - Excessive alcohol consumption has long been associated with cardiovascular disorders, including cardiomyopathy, hypertension, coronary artery disease, and stroke. However, recent evidence suggests that moderate alcohol intake can actually provide a measure of cardioprotection, particularly against coronary heart disease and ischemia-reperfusion injury. To explore the various dimensions of these opposing actions of alcohol, the National Institute on Alcohol Abuse and Alcoholism and the National Heart, Lung, and Blood Institute sponsored a state-of-the-art workshop on "Alcohol and the Cardiovascular System: Research Challenges and Opportunities" in Bethesda, Maryland, in May 2003. Speakers discussed the following topics: the epidemiology of alcohol and cardiovascular disease, clinical manifestations of alcohol, genetics of alcohol and cardiovascular disease, mechanisms underlying the molecular and cellular effects of alcohol, the application of new and emerging technology, and translation from discovery to therapeutic modalities of treatment. The panel concluded that future studies are needed to: 1) determine the role of genes and the environment in assessing mechanisms underlying the benefits of alcohol use and cardiovascular disease risk; 2) define the biological mechanisms underlying alcohol-induced peripheral vascular damage; 3) clarify the role of genetic variation in alcohol-metabolizing enzymes, genetic susceptibility, and pharmacogenomics in determining cardiovascular disease risk and effective treatment; 4) determine common mechanisms underlying alcohol-induced cardiovascular disease, such as oxidative stress and inflammation; 5) assess the role of insulin resistance, blood clotting, protein kinase C isoforms, and signal transduction mechanisms mediating alcohol's beneficial effects; and 6) explore the potential of stem cells in myocardial regeneration and repair in hearts damaged by alcohol. JF - Journal of the American College of Cardiology AU - Lucas, Diane L AU - Brown, Ricardo A AU - Wassef, Momtaz AU - Giles, Thomas D AD - Division of Metabolism and Health Effects, National Institute on Alcohol Abuse and Alcoholism/NIH, Bethesda, MD, USA. Y1 - 2005/06/21/ PY - 2005 DA - 2005 Jun 21 SP - 1916 EP - 1924 VL - 45 IS - 12 SN - 0735-1097, 0735-1097 KW - Ethanol KW - 3K9958V90M KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Ethanol -- pharmacology KW - Cardiovascular System -- drug effects KW - Cardiovascular Diseases -- chemically induced KW - Ethanol -- metabolism KW - Alcohol Drinking -- genetics KW - Cardiovascular Diseases -- genetics KW - Alcohol Drinking -- epidemiology KW - Cardiovascular Diseases -- prevention & control KW - Ethanol -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67949186?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+College+of+Cardiology&rft.atitle=Alcohol+and+the+cardiovascular+system%3A+research+challenges+and+opportunities.&rft.au=Lucas%2C+Diane+L%3BBrown%2C+Ricardo+A%3BWassef%2C+Momtaz%3BGiles%2C+Thomas+D&rft.aulast=Lucas&rft.aufirst=Diane&rft.date=2005-06-21&rft.volume=45&rft.issue=12&rft.spage=1916&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+College+of+Cardiology&rft.issn=07351097&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The novel mGluR2/3 agonist LY379268 attenuates cue-induced reinstatement of heroin seeking. AN - 67892655; 15931079 AB - In humans, drug-associated stimuli can provoke heroin relapse during abstinence. In rats, cues paired with heroin self-administration reinstate heroin seeking in a relapse model. The neurobiological mechanisms involved in this reinstatement, however, are largely unknown. Here, we determined the effect of LY379268, an mGluR2/3 agonist that decreases evoked glutamate release, on cue-induced reinstatement of heroin seeking. Systemic injections of LY379268 attenuated reinstatement of heroin seeking induced by exposure to a discrete tone-light cue that was previously paired with heroin infusions during self-administration training. In contrast, LY379268 had no effect on heroin self-administration. Results indicate that glutamate plays an important role in cue-induced reinstatement of heroin seeking and suggest that mGluR2/3 agonists should be considered for the treatment of opiate relapse. JF - Neuroreport AU - Bossert, Jennifer M AU - Busch, Robert F AU - Gray, Sarah M AD - Behavioral Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, NIH/DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. jbossert@intra.nida.nih.gov Y1 - 2005/06/21/ PY - 2005 DA - 2005 Jun 21 SP - 1013 EP - 1016 VL - 16 IS - 9 SN - 0959-4965, 0959-4965 KW - Amino Acids KW - 0 KW - Bridged Bicyclo Compounds, Heterocyclic KW - Excitatory Amino Acid Agonists KW - LY 379268 KW - Narcotics KW - Heroin KW - 70D95007SX KW - Index Medicus KW - Rats KW - Animals KW - Self Administration -- methods KW - Rats, Long-Evans KW - Dose-Response Relationship, Drug KW - Narcotics -- administration & dosage KW - Extinction, Psychological -- drug effects KW - Male KW - Heroin -- administration & dosage KW - Behavior, Animal KW - Conditioning, Operant -- drug effects KW - Bridged Bicyclo Compounds, Heterocyclic -- pharmacology KW - Heroin Dependence -- physiopathology KW - Heroin Dependence -- drug therapy KW - Cues KW - Excitatory Amino Acid Agonists -- pharmacology KW - Amino Acids -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67892655?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroreport&rft.atitle=The+novel+mGluR2%2F3+agonist+LY379268+attenuates+cue-induced+reinstatement+of+heroin+seeking.&rft.au=Bossert%2C+Jennifer+M%3BBusch%2C+Robert+F%3BGray%2C+Sarah+M&rft.aulast=Bossert&rft.aufirst=Jennifer&rft.date=2005-06-21&rft.volume=16&rft.issue=9&rft.spage=1013&rft.isbn=&rft.btitle=&rft.title=Neuroreport&rft.issn=09594965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-30 N1 - Date created - 2005-06-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - National Institutes of Health State-of-the-Science Conference Statement: Management of Menopause-Related Symptoms AN - 222241448; 15968015 AB - The National Institutes of Health State-of-the-Science conference statement on menopause is presented. The focus of the report is to identify menopausal symptoms and assess treatments for them on the basis of existing scientific evidence. JF - Annals of Internal Medicine AU - National Institutes of Health AD - National Institutes of Health Y1 - 2005/06/21/ PY - 2005 DA - 2005 Jun 21 SP - 1003 EP - 13 CY - Philadelphia PB - American College of Physicians VL - 142 IS - 12 SN - 00034819 KW - Medical Sciences KW - Conferences KW - Menopause KW - Women KW - Medical treatment KW - Estrogen Replacement Therapy KW - Cognition Disorders -- etiology KW - Cognition Disorders -- therapy KW - Humans KW - Sleep Disorders -- therapy KW - Quality of Life KW - Dyspareunia -- etiology KW - Mood Disorders -- etiology KW - Vasomotor System -- physiopathology KW - Decision Making KW - Postmenopause -- physiology KW - Dyspareunia -- therapy KW - Menopause -- physiology KW - Perimenopause -- physiology KW - Menopause -- drug effects KW - Mood Disorders -- therapy KW - Middle Aged KW - Sleep Disorders -- etiology KW - Female UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/222241448?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+Internal+Medicine&rft.atitle=National+Institutes+of+Health+State-of-the-Science+Conference+Statement%3A+Management+of+Menopause-Related+Symptoms&rft.au=National+Institutes+of+Health&rft.aulast=National+Institutes+of+Health&rft.aufirst=&rft.date=2005-06-21&rft.volume=142&rft.issue=12&rft.spage=1003&rft.isbn=&rft.btitle=&rft.title=Annals+of+Internal+Medicine&rft.issn=00034819&rft_id=info:doi/ LA - English DB - ProQuest Central N1 - Name - National Institutes of Health N1 - Copyright - Copyright American College of Physicians Jun 21, 2005 N1 - Last updated - 2013-04-25 ER - TY - JOUR T1 - Intracellular collagen degradation mediated by uPARAP/Endo180 is a major pathway of extracellular matrix turnover during malignancy. AN - 67958335; 15967816 AB - We recently reported that uPARAP/Endo180 can mediate the cellular uptake and lysosomal degradation of collagen by cultured fibroblasts. Here, we show that uPARAP/Endo180 has a key role in the degradation of collagen during mammary carcinoma progression. In the normal murine mammary gland, uPARAP/Endo180 is widely expressed in periductal fibroblast-like mesenchymal cells that line mammary epithelial cells. This pattern of uPARAP/Endo180 expression is preserved during polyomavirus middle T-induced mammary carcinogenesis, with strong uPARAP/Endo180 expression by mesenchymal cells embedded within the collagenous stroma surrounding nests of uPARAP/Endo180-negative tumor cells. Genetic ablation of uPARAP/Endo180 impaired collagen turnover that is critical to tumor expansion, as evidenced by the abrogation of cellular collagen uptake, tumor fibrosis, and blunted tumor growth. These studies identify uPARAP/Endo180 as a key mediator of collagen turnover in a pathophysiological context. JF - The Journal of cell biology AU - Curino, Alejandro C AU - Engelholm, Lars H AU - Yamada, Susan S AU - Holmbeck, Kenn AU - Lund, Leif R AU - Molinolo, Alfredo A AU - Behrendt, Niels AU - Nielsen, Boye Schnack AU - Bugge, Thomas H AD - Proteases and Tissue Remodeling Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/06/20/ PY - 2005 DA - 2005 Jun 20 SP - 977 EP - 985 VL - 169 IS - 6 SN - 0021-9525, 0021-9525 KW - Membrane Glycoproteins KW - 0 KW - Mrc2 protein, mouse KW - Receptors, Cell Surface KW - Collagen KW - 9007-34-5 KW - Index Medicus KW - Animals KW - Neoplasm Invasiveness KW - Disease Models, Animal KW - Polyomavirus KW - Mice KW - Mice, Knockout KW - Microscopy, Electron, Transmission KW - Cells, Cultured KW - Mammary Glands, Animal -- metabolism KW - Stromal Cells -- ultrastructure KW - Mammary Glands, Animal -- pathology KW - Mammary Glands, Animal -- ultrastructure KW - Molecular Sequence Data KW - Stromal Cells -- metabolism KW - Stromal Cells -- pathology KW - Female KW - Mammary Neoplasms, Experimental -- ultrastructure KW - Carcinoma -- ultrastructure KW - Extracellular Matrix -- metabolism KW - Collagen -- metabolism KW - Cell Transformation, Neoplastic -- metabolism KW - Mesoderm -- ultrastructure KW - Mammary Neoplasms, Experimental -- genetics KW - Cell Transformation, Neoplastic -- ultrastructure KW - Mammary Neoplasms, Experimental -- metabolism KW - Receptors, Cell Surface -- metabolism KW - Mesoderm -- pathology KW - Receptors, Cell Surface -- genetics KW - Extracellular Matrix -- ultrastructure KW - Mesoderm -- metabolism KW - Carcinoma -- metabolism KW - Cell Transformation, Neoplastic -- genetics KW - Membrane Glycoproteins -- metabolism KW - Membrane Glycoproteins -- genetics KW - Carcinoma -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67958335?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+cell+biology&rft.atitle=Intracellular+collagen+degradation+mediated+by+uPARAP%2FEndo180+is+a+major+pathway+of+extracellular+matrix+turnover+during+malignancy.&rft.au=Curino%2C+Alejandro+C%3BEngelholm%2C+Lars+H%3BYamada%2C+Susan+S%3BHolmbeck%2C+Kenn%3BLund%2C+Leif+R%3BMolinolo%2C+Alfredo+A%3BBehrendt%2C+Niels%3BNielsen%2C+Boye+Schnack%3BBugge%2C+Thomas+H&rft.aulast=Curino&rft.aufirst=Alejandro&rft.date=2005-06-20&rft.volume=169&rft.issue=6&rft.spage=977&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+cell+biology&rft.issn=00219525&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-22 N1 - Date created - 2005-06-21 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - X83536; GENBANK; X66473; BC072650 N1 - SuppNotes - Cited By: Biol Chem. 2004 Feb;385(2):103-36 [15101555] Exp Cell Res. 2004 Feb 1;293(1):106-16 [14729061] J Bone Joint Surg Br. 1972 May;54(2):351-9 [4338380] Arthritis Rheum. 1977 Mar;20(2):657-65 [849360] J Periodontal Res. 1977 Sep;12(5):378-86 [143520] Nature. 1980 Mar 6;284(5751):67-8 [6243750] Adv Cancer Res. 1985;44:139-266 [2930999] Matrix. 1989 Aug;9(4):266-76 [2552268] Connect Tissue Res. 1989;21(1-4):43-8; discussion 49-50 [2605953] Cell. 1991 Jan 25;64(2):327-36 [1703045] FASEB J. 1991 Oct;5(13):2814-23 [1916105] Mol Cell Biol. 1992 Mar;12(3):954-61 [1312220] Genes Chromosomes Cancer. 1992 Jul;5(1):14-20 [1384657] Ann Rheum Dis. 1993 Apr;52(4):278-84 [8484694] Breast Cancer Res Treat. 1995 Aug;35(2):173-86 [7647339] Acta Orthop Scand Suppl. 1995 Oct;266:61-5 [8553864] J Am Acad Dermatol. 1996 Feb;34(2 Pt 1):209-18 [8642084] Science. 1996 Aug 30;273(5279):1236-8 [8703060] Histochem J. 1996 Apr;28(4):229-45 [8762055] Oncogene. 1998 Jun 18;16(24):3097-104 [9671388] Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13453-8 [9811821] APMIS. 1999 Jan;107(1):11-8 [10190275] APMIS. 1999 Jan;107(1):120-7 [10190288] Cell. 1999 Oct 1;99(1):81-92 [10520996] J Cell Physiol. 2005 Oct;205(1):123-32 [15895410] Cell. 2000 Jan 7;100(1):57-70 [10647931] J Cell Sci. 2000 Mar;113 ( Pt 6):1021-32 [10683150] Mol Med Today. 2000 Apr;6(4):149-56 [10740253] Science. 2000 Aug 18;289(5482):1197-202 [10947988] Cancer Res. 2001 Jan 1;61(1):293-302 [11196177] Nature. 2001 May 17;411(6835):375-9 [11357145] Mol Biol Cell. 2001 May;12(5):1457-66 [11359935] Trends Cardiovasc Med. 2001 Jan;11(1):7-13 [11413046] Lab Invest. 2001 Oct;81(10):1403-14 [11598153] Cancer Res. 2001 Nov 15;61(22):8298-305 [11719463] Int J Cancer. 2002 Apr 10;98(5):656-64 [11920633] Nat Rev Cancer. 2002 Aug;2(8):563-72 [12154349] Nature. 2002 Dec 19-26;420(6917):860-7 [12490959] J Cell Biol. 2003 Mar 31;160(7):1009-15 [12668656] EMBO Rep. 2003 Jul;4(7):710-6 [12835757] Cell. 2003 Jul 11;114(1):33-45 [12859896] Am J Pathol. 2003 Nov;163(5):2113-26 [14578209] Eur Respir J. 2003 Nov;22(5):728-34 [14621076] Osteoarthritis Cartilage. 2004 Jan;12(1):74-82 [14697685] Curr Opin Cell Biol. 2004 Oct;16(5):558-64 [15363807] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The carcinogenicity of human papillomavirus types reflects viral evolution. AN - 67867766; 15914222 AB - Persistent infections with carcinogenic human papillomaviruses (HPV) cause virtually all cervical cancers. Cervical HPV types (n > 40) also represent the most common sexually transmitted agents, and most infections clear in 1-2 years. The risks of persistence and neoplastic progression to cancer and its histologic precursor, cervical intraepithelial neoplasia grade 3 (CIN3), differ markedly by HPV type. To study type-specific HPV natural history, we conducted a 10,000-woman, population-based prospective study of HPV infections and CIN3/cancer in Guanacaste, Costa Rica. By studying large numbers of women, we wished to separate viral persistence from neoplastic progression. We observed a strong concordance of newly-revised HPV evolutionary groupings with the separate risks of persistence and progression to CIN3/cancer. HPV16 was uniquely likely both to persist and to cause neoplastic progression when it persisted, making it a remarkably powerful human carcinogen that merits separate clinical consideration. Specifically, 19.9% of HPV16-infected women were diagnosed with CIN3/cancer at enrollment or during the five-year follow-up. Other carcinogenic types, many related to HPV16, were not particularly persistent but could cause neoplastic progression, at lower rates than HPV16, if they did persist. Some low-risk types were persistent but, nevertheless, virtually never caused CIN3. Therefore, carcinogenicity is not strictly a function of persistence. Separately, we noted that the carcinogenic HPV types code for an E5 protein, whereas most low-risk types either lack a definable homologous E5 ORF and/or a translation start codon for E5. These results present several clear clues and research directions in our ongoing efforts to understand HPV carcinogenesis. JF - Virology AU - Schiffman, Mark AU - Herrero, Rolando AU - Desalle, Rob AU - Hildesheim, Allan AU - Wacholder, Sholom AU - Rodriguez, Ana Cecilia AU - Bratti, Maria C AU - Sherman, Mark E AU - Morales, Jorge AU - Guillen, Diego AU - Alfaro, Mario AU - Hutchinson, Martha AU - Wright, Thomas C AU - Solomon, Diane AU - Chen, Zigui AU - Schussler, John AU - Castle, Philip E AU - Burk, Robert D AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, US Department of Health and Human Services, Bethesda, MA, USA. schiffmm@mail.nih.gov Y1 - 2005/06/20/ PY - 2005 DA - 2005 Jun 20 SP - 76 EP - 84 VL - 337 IS - 1 SN - 0042-6822, 0042-6822 KW - Index Medicus KW - Papillomavirus Infections -- epidemiology KW - Prospective Studies KW - Papillomavirus Infections -- complications KW - Humans KW - Female KW - Uterine Cervical Neoplasms -- etiology KW - Uterine Cervical Neoplasms -- prevention & control KW - Cocarcinogenesis KW - Papillomaviridae -- classification KW - Uterine Cervical Neoplasms -- epidemiology KW - Papillomaviridae -- physiology KW - Evolution, Molecular UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67867766?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Virology&rft.atitle=The+carcinogenicity+of+human+papillomavirus+types+reflects+viral+evolution.&rft.au=Schiffman%2C+Mark%3BHerrero%2C+Rolando%3BDesalle%2C+Rob%3BHildesheim%2C+Allan%3BWacholder%2C+Sholom%3BRodriguez%2C+Ana+Cecilia%3BBratti%2C+Maria+C%3BSherman%2C+Mark+E%3BMorales%2C+Jorge%3BGuillen%2C+Diego%3BAlfaro%2C+Mario%3BHutchinson%2C+Martha%3BWright%2C+Thomas+C%3BSolomon%2C+Diane%3BChen%2C+Zigui%3BSchussler%2C+John%3BCastle%2C+Philip+E%3BBurk%2C+Robert+D&rft.aulast=Schiffman&rft.aufirst=Mark&rft.date=2005-06-20&rft.volume=337&rft.issue=1&rft.spage=76&rft.isbn=&rft.btitle=&rft.title=Virology&rft.issn=00426822&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-01 N1 - Date created - 2005-05-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Proteomic Identification of Potential Susceptibility Factors in Drug-Induced Liver Disease AN - 17536287; 6397431 AB - Drug-induced liver disease (DILD) causes significant morbidity and mortality and impairs new drug development. Currently, no known criteria can predict whether a drug will cause DILD or what risk factors make an individual susceptible. Although it has been shown in mouse studies that the disruption of key regulatory factors, such as cyclooxygenase-2 (COX-2), interleukin (IL)-6, and IL-10, increased susceptibility to DILD caused by acetaminophen (APAP), no single factor seems to be absolute. As an approach to better understand the multifactorial basis of DILD, we compared the hepatic proteome of mice that are resistant (SJL) and susceptible (C57B1/6) to APAP-induced liver disease (AILD), using solution-based isotope-coded affinity tag (ICAT) liquid chromatography mass spectrometry. Several novel factors were identified that were more highly expressed in the livers of SJL mice, including those involved in stress response, cell proliferation and tissue regeneration, and protein modification, implicating these proteins as potential hepatoprotective factors. There was also a selective loss of several mitochondrial proteins from the livers of the susceptible C57B1/6 mice, suggesting that the loss of functional mitochondria may indeed play a role in AILD. These findings indicate that comparative hepatic proteomic analyses of susceptible and resistant mouse strains may provide a global approach for identifying potential risk factors and mechanistic pathways responsible for DILD. JF - Chemical Research in Toxicology AU - Welch, K D AU - Wen, B AU - Goodlett AU - Yi, E C AU - Lee, H AU - Reilly, T P AU - Nelson, S D AU - Pohl, L R AD - Molecular and Cellular Toxicology Section, Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA Y1 - 2005/06/20/ PY - 2005 DA - 2005 Jun 20 SP - 924 EP - 933 VL - 18 IS - 6 SN - 0893-228X, 0893-228X KW - Toxicology Abstracts KW - Cyclooxygenase-2 KW - Liver diseases KW - Stress KW - Mitochondria KW - Drug development KW - Interleukin 10 KW - Mass spectroscopy KW - Liquid chromatography KW - Risk factors KW - proteomics KW - Cell proliferation KW - Acetaminophen KW - X 24115:Pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17536287?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemical+Research+in+Toxicology&rft.atitle=Proteomic+Identification+of+Potential+Susceptibility+Factors+in+Drug-Induced+Liver+Disease&rft.au=Welch%2C+K+D%3BWen%2C+B%3BGoodlett%3BYi%2C+E+C%3BLee%2C+H%3BReilly%2C+T+P%3BNelson%2C+S+D%3BPohl%2C+L+R&rft.aulast=Welch&rft.aufirst=K&rft.date=2005-06-20&rft.volume=18&rft.issue=6&rft.spage=924&rft.isbn=&rft.btitle=&rft.title=Chemical+Research+in+Toxicology&rft.issn=0893228X&rft_id=info:doi/10.1021%2Ftx050011b LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Liver diseases; Risk factors; Mitochondria; Cyclooxygenase-2; proteomics; Drug development; Mass spectroscopy; Interleukin 10; Acetaminophen; Stress; Liquid chromatography; Cell proliferation DO - http://dx.doi.org/10.1021/tx050011b ER - TY - JOUR T1 - Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C. AN - 67928351; 15845534 AB - Human families with single amino acid mutations in nonmuscle myosin heavy chain (NMHC) II-A (MYH9) and II-C (MYH14) have been described as have mice generated with a point mutation in NMHC II-B (MYH10). These mutations (R702C and N93K in human NMHC II-A, R709C in murine NMHC II-B, and R726S in human NMHC II-C) result in phenotypes affecting kidneys, platelets, and leukocytes (II-A), heart and brain (II-B), and the inner ear (II-C). To better understand the mechanisms underlying these defects, we characterized the in vitro activity of mutated and wild-type baculovirus-expressed heavy meromyosin (HMM) II-B and II-C. We also expressed two alternatively spliced isoforms of NMHC II-C which differ by inclusion/exclusion of eight amino acids in loop 1, with and without mutations. Comparison of the actin-activated MgATPase activity and in vitro motility shows that mutation of residues Asn-97 and Arg-709 in HMM II-B and the homologous residue Arg-722 (Arg-730 in the alternatively spliced isoform) in HMM II-C decreases both parameters but affects in vitro motility more severely. Analysis of the transient kinetics of the HMM II-B R709C mutant shows an extremely tight affinity of HMM for ADP and a very slow release of ADP from acto-HMM. Although mutations generally decreased HMM activity, the R730S mutation in HMM II-C, unlike the R730C mutation, had no effect on actin-activated MgATPase activity but decreased the rate of in vitro motility by 75% compared with wild type. Insertion of eight amino acids into the HMM II-C heavy chain increases both actin-activated MgATPase activity and in vitro motility. JF - The Journal of biological chemistry AU - Kim, Kye-Young AU - Kovács, Mihály AU - Kawamoto, Sachiyo AU - Sellers, James R AU - Adelstein, Robert S AD - Laboratory of Molecular Cardiology and Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/06/17/ PY - 2005 DA - 2005 Jun 17 SP - 22769 EP - 22775 VL - 280 IS - 24 SN - 0021-9258, 0021-9258 KW - Actins KW - 0 KW - MYH14 protein, human KW - Myh14 protein, mouse KW - Myosin Subfragments KW - Protein Isoforms KW - Recombinant Proteins KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - Asparagine KW - 7006-34-0 KW - Arginine KW - 94ZLA3W45F KW - Ca(2+) Mg(2+)-ATPase KW - EC 3.6.1.- KW - Myosin Type II KW - Nonmuscle Myosin Type IIB KW - Myosin Heavy Chains KW - EC 3.6.4.1 KW - Myosins KW - Index Medicus KW - Animals KW - Models, Molecular KW - Arginine -- chemistry KW - Dose-Response Relationship, Drug KW - Adenosine Diphosphate -- chemistry KW - Humans KW - Myosin Subfragments -- chemistry KW - Mice KW - Insects KW - Phenotype KW - Mutagenesis, Site-Directed KW - Ca(2+) Mg(2+)-ATPase -- chemistry KW - Genetic Vectors KW - Kinetics KW - Point Mutation KW - Myosins -- chemistry KW - Recombinant Proteins -- chemistry KW - Protein Structure, Tertiary KW - Actins -- chemistry KW - Asparagine -- chemistry KW - Nonmuscle Myosin Type IIB -- chemistry KW - Myosin Heavy Chains -- genetics KW - Myosin Heavy Chains -- chemistry KW - Alternative Splicing KW - Nonmuscle Myosin Type IIB -- genetics KW - Mutation KW - Myosin Type II -- genetics KW - Myosin Type II -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67928351?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Disease-associated+mutations+and+alternative+splicing+alter+the+enzymatic+and+motile+activity+of+nonmuscle+myosins+II-B+and+II-C.&rft.au=Kim%2C+Kye-Young%3BKov%C3%A1cs%2C+Mih%C3%A1ly%3BKawamoto%2C+Sachiyo%3BSellers%2C+James+R%3BAdelstein%2C+Robert+S&rft.aulast=Kim&rft.aufirst=Kye-Young&rft.date=2005-06-17&rft.volume=280&rft.issue=24&rft.spage=22769&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-19 N1 - Date created - 2005-06-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Role of Toll-like receptor-4 in genetic susceptibility to lung injury induced by residual oil fly ash. AN - 67945063; 15784698 AB - The mechanisms of susceptibility to particle-induced lung injury are not clearly understood. To evaluate the contribution of genetic background to pulmonary pathogenesis, we compared the lung injury responses to residual oil fly ash (ROFA) in inbred mouse strains and calculated heritability estimates. Significant interstrain (genetic) variation was observed in ROFA-induced lung inflammation and hyperpermeability phenotypes; broad-sense heritability ranged from approximately 0.43 to 0.62, and the coefficient of genetic determination ranged from 0.28 to 0.45. C3H/HeJ (HeJ) mice were most resistant to the ROFA-induced injury responses. This was particularly important, as HeJ mice contain a dominant negative mutation in Toll-like receptor-4 (Tlr4). We then characterized ROFA-induced injury and TLR4 signaling in HeJ mice and its coisogenic strain C3H/HeOuJ (OuJ; Tlr4 normal) to understand the potential role of Tlr4 in this model. ROFA-induced lung injury was significantly greater in OuJ mice compared with HeJ mice. ROFA also significantly enhanced transcript and protein levels of lung TLR4 in OuJ but not in HeJ mice. Greater activation of downstream signal molecules (i.e., MYD88, TRAF6, IRAK-1, NF-kappaB, MAPK, AP-1) was observed in OuJ mice than in HeJ mice before the development of ROFA-induced pulmonary injury. Putative TLR4-dependent inflammatory genes that were differentially induced by ROFA in the two strains include interleukin-1beta and tumor necrosis factor-alpha. Results support an important contribution of genetic background to particle-mediated lung injury, and Tlr4 is a candidate susceptibility gene. JF - Physiological genomics AU - Cho, Hye-Youn AU - Jedlicka, Anne E AU - Clarke, Robert AU - Kleeberger, Steven R AD - Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA. cho2@niehs.nih.gov Y1 - 2005/06/16/ PY - 2005 DA - 2005 Jun 16 SP - 108 EP - 117 VL - 22 IS - 1 KW - Coal Ash KW - 0 KW - Cytokines KW - NF-kappa B KW - Particulate Matter KW - RNA, Messenger KW - Toll-Like Receptor 4 KW - Carbon KW - 7440-44-0 KW - Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Index Medicus KW - Animals KW - Mitogen-Activated Protein Kinases -- metabolism KW - Inflammation -- genetics KW - Cytokines -- metabolism KW - Mice KW - RNA, Messenger -- genetics KW - Genes, MHC Class II -- genetics KW - Mice, Inbred Strains KW - RNA, Messenger -- metabolism KW - Lung Injury KW - Signal Transduction -- genetics KW - Species Specificity KW - Male KW - NF-kappa B -- metabolism KW - Particulate Matter -- toxicity KW - Lung Diseases -- genetics KW - Lung Diseases -- chemically induced KW - Toll-Like Receptor 4 -- genetics KW - Genetic Predisposition to Disease -- genetics KW - Lung Diseases -- pathology KW - Lung -- drug effects KW - Toll-Like Receptor 4 -- metabolism KW - Carbon -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67945063?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Physiological+genomics&rft.atitle=Role+of+Toll-like+receptor-4+in+genetic+susceptibility+to+lung+injury+induced+by+residual+oil+fly+ash.&rft.au=Cho%2C+Hye-Youn%3BJedlicka%2C+Anne+E%3BClarke%2C+Robert%3BKleeberger%2C+Steven+R&rft.aulast=Cho&rft.aufirst=Hye-Youn&rft.date=2005-06-16&rft.volume=22&rft.issue=1&rft.spage=108&rft.isbn=&rft.btitle=&rft.title=Physiological+genomics&rft.issn=1531-2267&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-08 N1 - Date created - 2005-06-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Enhancement of in vitro and in vivo tumor cell radiosensitivity by the DNA methylation inhibitor zebularine. AN - 67937014; 15958643 AB - Aberrant DNA hypermethylation is a frequent finding in tumor cells, which has suggested that inhibition of DNA methylation may be an effective cancer treatment strategy. Because DNA methylation affects gene expression and chromatin structure, parameters considered to influence radioresponse, we investigated the effects of the DNA methylation inhibitor zebularine on the radiosensitivity of human tumor cells. Three human tumor cell lines were used in this study (MiaPaCa, DU145, and U251) and the methylation status of three genes frequently hypermethylated in tumor cells (RASSF1A, HIC-1, and 14-3-3sigma) was determined as a function of zebularine exposure. Zebularine resulted in DNA demethylation in a time-dependent manner, with the maximum loss of methylation detected by 48 hours. Treatment of cells with zebularine for 48 hours also resulted in an increase in radiosensitivity with dose enhancement factors of >1.5. As a measure of radiation-induced DNA damage, gammaH2AX expression was determined. Whereas zebularine had no effect on radiation-induced gammaH2AX foci at 1 hour, the number of gammaH2AX foci per cell was significantly greater in the zebularine-treated cells at 24 hours after irradiation, suggesting the presence of unrepaired DNA damage. Zebularine administration to mice reactivated gene expression in U251 xenografts; irradiation of U251 tumors in mice treated with zebularine resulted in an increase in radiation-induced tumor growth delay. These results indicate that zebularine can enhance tumor cell radiosensitivity in vitro and in vivo and suggest that this effect may involve an inhibition of DNA repair. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Dote, Hideaki AU - Cerna, David AU - Burgan, William E AU - Carter, Donna J AU - Cerra, Michael A AU - Hollingshead, Melinda G AU - Camphausen, Kevin AU - Tofilon, Philip J AD - Molecular Radiation Therapeutics Branch, National Cancer Institute, Bethesda, Maryland, USA. Y1 - 2005/06/15/ PY - 2005 DA - 2005 Jun 15 SP - 4571 EP - 4579 VL - 11 IS - 12 SN - 1078-0432, 1078-0432 KW - 14-3-3 Proteins KW - 0 KW - Biomarkers, Tumor KW - DNA-Binding Proteins KW - HIC1 protein, human KW - Kruppel-Like Transcription Factors KW - Neoplasm Proteins KW - RASSF1 protein, human KW - RNA, Messenger KW - Transcription Factors KW - Tumor Suppressor Proteins KW - Cytidine KW - 5CSZ8459RP KW - pyrimidin-2-one beta-ribofuranoside KW - 7A9Y5SX0GY KW - Exonucleases KW - EC 3.1.- KW - Exoribonucleases KW - SFN protein, human KW - Index Medicus KW - Biomarkers, Tumor -- genetics KW - Animals KW - Exonucleases -- genetics KW - Humans KW - Tumor Suppressor Proteins -- genetics KW - DNA-Binding Proteins -- genetics KW - Mice, Nude KW - RNA, Messenger -- genetics KW - Cell Survival -- drug effects KW - Apoptosis -- drug effects KW - Neoplasm Proteins -- genetics KW - Time Factors KW - Male KW - Xenograft Model Antitumor Assays -- methods KW - Combined Modality Therapy KW - Apoptosis -- radiation effects KW - Cell Line, Tumor KW - Mice KW - Dose-Response Relationship, Radiation KW - Reverse Transcriptase Polymerase Chain Reaction KW - Transcription Factors -- genetics KW - Gene Expression Regulation, Neoplastic -- drug effects KW - RNA, Messenger -- metabolism KW - Cell Survival -- radiation effects KW - Neoplasms, Experimental -- therapy KW - Neoplasms, Experimental -- genetics KW - DNA Methylation -- drug effects KW - Cytidine -- pharmacology KW - Neoplasms, Experimental -- pathology KW - Cytidine -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67937014?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Enhancement+of+in+vitro+and+in+vivo+tumor+cell+radiosensitivity+by+the+DNA+methylation+inhibitor+zebularine.&rft.au=Dote%2C+Hideaki%3BCerna%2C+David%3BBurgan%2C+William+E%3BCarter%2C+Donna+J%3BCerra%2C+Michael+A%3BHollingshead%2C+Melinda+G%3BCamphausen%2C+Kevin%3BTofilon%2C+Philip+J&rft.aulast=Dote&rft.aufirst=Hideaki&rft.date=2005-06-15&rft.volume=11&rft.issue=12&rft.spage=4571&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-17 N1 - Date created - 2005-06-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Imaging brain mu-opioid receptors in abstinent cocaine users: time course and relation to cocaine craving. AN - 67927566; 15953495 AB - Cocaine treatment upregulates brain mu-opioid receptors (mOR) in animals. Human data regarding this phenomenon are limited. We previously used positron emission tomography (PET) with [11C]-carfentanil to show increased mOR binding in brain regions of 10 cocaine-dependent men after 1 and 28 days of abstinence. Regional brain mOR binding potential (BP) was measured with [11C]carfentanil PET scanning in 17 cocaine users over 12 weeks of abstinence on a research ward and in 16 healthy control subjects. Mu-opioid receptor BP was increased in the frontal, anterior cingulate, and lateral temporal cortex after 1 day of abstinence. Mu-opioid receptor BP remained elevated in the first two regions after 1 week and in the anterior cingulate and anterior frontal cortex after 12 weeks. Increased binding in some regions at 1 day and 1 week was positively correlated with self-reported cocaine craving. Mu-opioid receptor BP was significantly correlated with percentage of days with cocaine use and amount of cocaine used per day of use during the 2 weeks before admission and with urine benzoylecgonine concentration at the first PET scan. These results suggest that chronic cocaine use influences endogenous opioid systems in the human brain and might explain mechanisms of cocaine craving and reinforcement. JF - Biological psychiatry AU - Gorelick, David A AU - Kim, Yu Kyeong AU - Bencherif, Badreddine AU - Boyd, Susan J AU - Nelson, Richard AU - Copersino, Marc AU - Endres, Christopher J AU - Dannals, Robert F AU - Frost, J James AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland 21224, USA. dgorelic@intra.nida.nih.gov Y1 - 2005/06/15/ PY - 2005 DA - 2005 Jun 15 SP - 1573 EP - 1582 VL - 57 IS - 12 SN - 0006-3223, 0006-3223 KW - Analgesics, Opioid KW - 0 KW - Carbon Isotopes KW - Receptors, Opioid, mu KW - carfentanil KW - LA9DTA2L8F KW - Fentanyl KW - UF599785JZ KW - Index Medicus KW - Magnetic Resonance Imaging KW - Humans KW - Linear Models KW - Retrospective Studies KW - Analgesics, Opioid -- pharmacokinetics KW - Protein Binding KW - Brain Mapping KW - Adult KW - Carbon Isotopes -- pharmacokinetics KW - Middle Aged KW - Time Factors KW - Image Processing, Computer-Assisted KW - Female KW - Functional Laterality KW - Male KW - Fentanyl -- pharmacokinetics KW - Positron-Emission Tomography -- methods KW - Cocaine-Related Disorders -- pathology KW - Brain -- pathology KW - Brain -- drug effects KW - Brain -- metabolism KW - Receptors, Opioid, mu -- metabolism KW - Fentanyl -- analogs & derivatives KW - Cocaine-Related Disorders -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67927566?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biological+psychiatry&rft.atitle=Imaging+brain+mu-opioid+receptors+in+abstinent+cocaine+users%3A+time+course+and+relation+to+cocaine+craving.&rft.au=Gorelick%2C+David+A%3BKim%2C+Yu+Kyeong%3BBencherif%2C+Badreddine%3BBoyd%2C+Susan+J%3BNelson%2C+Richard%3BCopersino%2C+Marc%3BEndres%2C+Christopher+J%3BDannals%2C+Robert+F%3BFrost%2C+J+James&rft.aulast=Gorelick&rft.aufirst=David&rft.date=2005-06-15&rft.volume=57&rft.issue=12&rft.spage=1573&rft.isbn=&rft.btitle=&rft.title=Biological+psychiatry&rft.issn=00063223&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-02 N1 - Date created - 2005-06-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dexrazoxane for the prevention of cardiomyopathy in anthracycline treated pediatric cancer patients. AN - 67811551; 15700251 AB - Anthracyclines play a major role in chemotherapeutic regimens for a variety of childhood cancers, but produce dose-related cardiotoxicity. Dexrazoxane, a chelating agent that binds iron intracellularly, has been cautiously included in anthracycline-based regimens. Our understanding of anthracycline and dexrazoxane pharmacokinetics in children is very limited. In addition, the administration schedule used for adults (bolus dexrazoxane prior to bolus anthracycline) may not be the best to attain both short- and long-term cardioprotection. Dexrazoxane could diminish the anti-tumor activity of and/or increase toxicities from anthracyclines. Pediatric oncologists must be assured this intervention does not diminish the success in curing children with cancer. Copyright 2005 Wiley-Liss, Inc. JF - Pediatric blood & cancer AU - Anderson, Barry AD - Clinical Investigations Branch, Pediatric Section, Cancer Therapy Evaluation Program, National Cancer Institute, Rockville, Maryland 20852, USA. andersonb@ctep.nci.nih.gov Y1 - 2005/06/15/ PY - 2005 DA - 2005 Jun 15 SP - 584 EP - 588 VL - 44 IS - 7 SN - 1545-5009, 1545-5009 KW - Anthracyclines KW - 0 KW - Antibiotics, Antineoplastic KW - Cardiovascular Agents KW - Razoxane KW - 5AR83PR647 KW - Index Medicus KW - Humans KW - Child KW - Razoxane -- therapeutic use KW - Neoplasms -- drug therapy KW - Cardiovascular Agents -- therapeutic use KW - Cardiomyopathies -- drug therapy KW - Cardiomyopathies -- chemically induced KW - Anthracyclines -- adverse effects KW - Antibiotics, Antineoplastic -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67811551?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatric+blood+%26+cancer&rft.atitle=Dexrazoxane+for+the+prevention+of+cardiomyopathy+in+anthracycline+treated+pediatric+cancer+patients.&rft.au=Anderson%2C+Barry&rft.aulast=Anderson&rft.aufirst=Barry&rft.date=2005-06-15&rft.volume=44&rft.issue=7&rft.spage=584&rft.isbn=&rft.btitle=&rft.title=Pediatric+blood+%26+cancer&rft.issn=15455009&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-09 N1 - Date created - 2005-05-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functional roles of NMDA receptor NR2A and NR2B subunits in the acute intoxicating effects of ethanol in mice. AN - 67720413; 15803501 AB - The present study examined the roles of NR2A and NR2B subunit-containing NMDA receptors in the mediation of the sedative/hypnotic effects of ethanol in mice. The ability of the competitive NMDA antagonist, CGP-37849 (0, 1, or 3 mg/kg), and the NR2B-selective antagonist, Ro 25-6981 (0, 3, or 10 mg/kg), to alter (3 g/kg) ethanol-induced sleep time was measured in C57BL/6J mice and NR2A knockout (KO) mice. The results show that pretreatment with either antagonist significantly potentiated the sedative/hypnotic effects of ethanol in C57BL/6J mice. These effects were not significantly altered in NR2A KO mice. Basal sleep time responses to ethanol were also normal in NR2A KO mice. These findings confirm a major role for NMDA receptors in the acute intoxicating actions of ethanol and provide tentative support for a prepotent role of the NR2B subunit in these effects. JF - Synapse (New York, N.Y.) AU - Boyce-Rustay, Janel M AU - Holmes, Andrew AD - Section on Behavioral Science and Genetics, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA. boycej@mail.nih.gov Y1 - 2005/06/15/ PY - 2005 DA - 2005 Jun 15 SP - 222 EP - 225 VL - 56 IS - 4 SN - 0887-4476, 0887-4476 KW - Central Nervous System Depressants KW - 0 KW - NR2A NMDA receptor KW - NR2B NMDA receptor KW - Phenols KW - Piperidines KW - Receptors, N-Methyl-D-Aspartate KW - Ro 25-6981 KW - 2-amino-4-methyl-5-phosphono-3-pentenoic acid KW - 137424-81-8 KW - Ethanol KW - 3K9958V90M KW - 2-Amino-5-phosphonovalerate KW - 76726-92-6 KW - Index Medicus KW - Piperidines -- pharmacology KW - Animals KW - Drug Interactions KW - Phenols -- pharmacology KW - Dose-Response Relationship, Drug KW - Mice, Inbred C57BL KW - Mice KW - Male KW - Mice, Knockout KW - Receptors, N-Methyl-D-Aspartate -- physiology KW - Central Nervous System Depressants -- toxicity KW - Sleep -- drug effects KW - 2-Amino-5-phosphonovalerate -- pharmacology KW - 2-Amino-5-phosphonovalerate -- analogs & derivatives KW - Receptors, N-Methyl-D-Aspartate -- antagonists & inhibitors KW - Ethanol -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67720413?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Synapse+%28New+York%2C+N.Y.%29&rft.atitle=Functional+roles+of+NMDA+receptor+NR2A+and+NR2B+subunits+in+the+acute+intoxicating+effects+of+ethanol+in+mice.&rft.au=Boyce-Rustay%2C+Janel+M%3BHolmes%2C+Andrew&rft.aulast=Boyce-Rustay&rft.aufirst=Janel&rft.date=2005-06-15&rft.volume=56&rft.issue=4&rft.spage=222&rft.isbn=&rft.btitle=&rft.title=Synapse+%28New+York%2C+N.Y.%29&rft.issn=08874476&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-29 N1 - Date created - 2005-04-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Allele-specific germ cell epimutation in the spacer promoter of the 45S ribosomal RNA gene after Cr(III) exposure AN - 17504831; 6393475 AB - Paternal exposure of mice to Cr(III) causes increased tumor risk in offspring; an epigenetic mechanism has been hypothesized. Representational difference analysis of gene methylation in sperm revealed hypomethylation in the 45S ribosomal RNA (rRNA) gene after Cr(III) exposure, compared with controls. The most striking effects were seen in the rRNA spacer promoter, a region in the intergenic region of rRNA gene clusters that can influence transcription. Methylation of the rRNA spacer promoter has not been studied heretofore. Sperm DNAs from Cr(III)-treated and control mice were modified by the bisulfite method followed by PCR amplification of the spacer promoter, including 27 CpG sites. Cloning and dideoxy sequencing identified sequence variants (T or G at base -2214) in the spacer promoter. The T allele had less DNA methylation than the G allele in control mice (17 of 17 clones vs. 42 of 72 clones, P = 0.0004). In spite of diversity of sperm DNA methylation patterns, the DNA clones from Cr(III)-exposed mice had fewer methylated CpG sites, by an average of 19% (P < 0.0001). This difference was limited to the G allele. The pyrosequencing technique was applied to quantify the percentage of methylation directly from amplified PCR products. Strikingly, for nine CpG sites including the spacer promoter core region, hypomethylation was highly significant in the Cr(III)-treated group (paired T test, P < 0.0001). Thus, one allele of the 45S rRNA spacer promoter is hypomethylated in sperm germ cells after Cr(III) exposure. This epimutation may lead to increase of tumor risk in the offspring. JF - Toxicology and Applied Pharmacology AU - Shiao, Y H AU - Crawford, E B AU - Anderson, L M AU - Patel, P AU - Ko, K AD - West 7th Street, Building 538, Room 205, National Cancer Institute, Frederick, MD 21702, USA, shiao@mail.ncifrcf.gov Y1 - 2005/06/15/ PY - 2005 DA - 2005 Jun 15 SP - 290 EP - 296 VL - 205 IS - 3 SN - 0041-008X, 0041-008X KW - Toxicology Abstracts KW - Chromium KW - Bisulfite KW - Germ cells KW - Spacer KW - CpG islands KW - Sperm KW - Promoters KW - epigenetics KW - rRNA 45S KW - Gene clusters KW - DNA methylation KW - Polymerase chain reaction KW - X 24165:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17504831?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Allele-specific+germ+cell+epimutation+in+the+spacer+promoter+of+the+45S+ribosomal+RNA+gene+after+Cr%28III%29+exposure&rft.au=Shiao%2C+Y+H%3BCrawford%2C+E+B%3BAnderson%2C+L+M%3BPatel%2C+P%3BKo%2C+K&rft.aulast=Shiao&rft.aufirst=Y&rft.date=2005-06-15&rft.volume=205&rft.issue=3&rft.spage=290&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.10.017 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Promoters; Spacer; DNA methylation; Sperm; rRNA 45S; CpG islands; Germ cells; Polymerase chain reaction; Gene clusters; epigenetics; Chromium; Bisulfite DO - http://dx.doi.org/10.1016/j.taap.2004.10.017 ER - TY - JOUR T1 - Induced hypertension improves cerebral blood flow in acute ischemic stroke. AN - 67935135; 15955961 JF - Neurology AU - Chalela, Julio A AU - Dunn, Billy AU - Todd, Jason W AU - Warach, Steven AD - Stroke Diagnostics and Therapeutics Section, NINDS/NIH, 10 Center Dr., Room B1D733, MSC 1063, Bethesda, MD 20892, USA. chalelaj@ninds.nih.gov Y1 - 2005/06/14/ PY - 2005 DA - 2005 Jun 14 SP - 1979 VL - 64 IS - 11 KW - Vasoconstrictor Agents KW - 0 KW - Phenylephrine KW - 1WS297W6MV KW - Abridged Index Medicus KW - Index Medicus KW - Magnetic Resonance Imaging KW - Acute Disease KW - Infarction, Anterior Cerebral Artery -- drug therapy KW - Injections, Intravenous KW - Humans KW - Treatment Outcome KW - Aged KW - Male KW - Phenylephrine -- therapeutic use KW - Infarction, Anterior Cerebral Artery -- physiopathology KW - Infarction, Anterior Cerebral Artery -- pathology KW - Stroke -- drug therapy KW - Hypertension -- chemically induced KW - Blood Pressure -- physiology KW - Brain Ischemia -- drug therapy KW - Stroke -- pathology KW - Brain Ischemia -- pathology KW - Cerebrovascular Circulation -- physiology KW - Cerebrovascular Circulation -- drug effects KW - Stroke -- physiopathology KW - Blood Pressure -- drug effects KW - Vasoconstrictor Agents -- therapeutic use KW - Brain Ischemia -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67935135?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurology&rft.atitle=Induced+hypertension+improves+cerebral+blood+flow+in+acute+ischemic+stroke.&rft.au=Chalela%2C+Julio+A%3BDunn%2C+Billy%3BTodd%2C+Jason+W%3BWarach%2C+Steven&rft.aulast=Chalela&rft.aufirst=Julio&rft.date=2005-06-14&rft.volume=64&rft.issue=11&rft.spage=1979&rft.isbn=&rft.btitle=&rft.title=Neurology&rft.issn=1526-632X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-27 N1 - Date created - 2005-06-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase I and pharmacokinetic study of MS-275, a histone deacetylase inhibitor, in patients with advanced and refractory solid tumors or lymphoma. AN - 67921761; 15851766 AB - The objective of this study was to define the maximum-tolerated dose (MTD), the recommended phase II dose, the dose-limiting toxicity, and determine the pharmacokinetic (PK) and pharmacodynamic profiles of MS-275. Patients with advanced solid tumors or lymphoma were treated with MS-275 orally initially on a once daily x 28 every 6 weeks (daily) and later on once every-14-days (q14-day) schedules. The starting dose was 2 mg/m2 and the dose was escalated in three- to six-patient cohorts based on toxicity assessments. With the daily schedule, the MTD was exceeded at the first dose level. Preliminary PK analysis suggested the half-life of MS-275 in humans was 39 to 80 hours, substantially longer than predicted by preclinical studies. With the q14-day schedule, 28 patients were treated. The MTD was 10 mg/m2 and dose-limiting toxicities were nausea, vomiting, anorexia, and fatigue. Exposure to MS-275 was dose dependent, suggesting linear PK. Increased histone H3 acetylation in peripheral-blood mononuclear-cells was apparent at all dose levels by immunofluorescence analysis. Ten of 29 patients remained on treatment for > or = 3 months. The MS-275 oral formulation on the daily schedule was intolerable at a dose and schedule explored. The q14-day schedule is reasonably well tolerated. Histone deacetylase inhibition was observed in peripheral-blood mononuclear-cells. Based on PK data from the q14-day schedule, a more frequent dosing schedule, weekly x 4, repeated every 6 weeks is presently being evaluated. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Ryan, Qin C AU - Headlee, Donna AU - Acharya, Milin AU - Sparreboom, Alex AU - Trepel, Jane B AU - Ye, Joseph AU - Figg, William D AU - Hwang, Kyunghwa AU - Chung, Eun Joo AU - Murgo, Anthony AU - Melillo, Giovanni AU - Elsayed, Yusri AU - Monga, Manish AU - Kalnitskiy, Mikhail AU - Zwiebel, James AU - Sausville, Edward A AD - Clinical Trials Unit, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD, USA. Y1 - 2005/06/10/ PY - 2005 DA - 2005 Jun 10 SP - 3912 EP - 3922 VL - 23 IS - 17 SN - 0732-183X, 0732-183X KW - Benzamides KW - 0 KW - Enzyme Inhibitors KW - Histone Deacetylase Inhibitors KW - Pyridines KW - entinostat KW - 1ZNY4FKK9H KW - Index Medicus KW - Administration, Oral KW - Drug Administration Schedule KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Maximum Tolerated Dose KW - Male KW - Female KW - Neoplasms -- drug therapy KW - Enzyme Inhibitors -- administration & dosage KW - Pyridines -- administration & dosage KW - Neoplasm Recurrence, Local -- drug therapy KW - Pyridines -- pharmacokinetics KW - Benzamides -- pharmacokinetics KW - Lymphoma -- drug therapy KW - Enzyme Inhibitors -- pharmacokinetics KW - Lymphoma -- metabolism KW - Benzamides -- administration & dosage KW - Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67921761?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Phase+I+and+pharmacokinetic+study+of+MS-275%2C+a+histone+deacetylase+inhibitor%2C+in+patients+with+advanced+and+refractory+solid+tumors+or+lymphoma.&rft.au=Ryan%2C+Qin+C%3BHeadlee%2C+Donna%3BAcharya%2C+Milin%3BSparreboom%2C+Alex%3BTrepel%2C+Jane+B%3BYe%2C+Joseph%3BFigg%2C+William+D%3BHwang%2C+Kyunghwa%3BChung%2C+Eun+Joo%3BMurgo%2C+Anthony%3BMelillo%2C+Giovanni%3BElsayed%2C+Yusri%3BMonga%2C+Manish%3BKalnitskiy%2C+Mikhail%3BZwiebel%2C+James%3BSausville%2C+Edward+A&rft.aulast=Ryan&rft.aufirst=Qin&rft.date=2005-06-10&rft.volume=23&rft.issue=17&rft.spage=3912&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-07 N1 - Date created - 2005-06-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - HIV/AIDS in women: an expanding epidemic. AN - 67920442; 15947174 AB - More than 20 years into the human immunodeficiency virus-type 1 (HIV-1) epidemic, women account for nearly half of the 40 million people living with HIV-1 worldwide, with an even higher proportion existing in developing countries. Social determinants of female vulnerability to HIV-1 include gender disparities, poverty, cultural and sexual norms, lack of education, and violence. Women are also more susceptible to HIV-1 because of hormonal changes, vaginal microbial ecology and physiology, and a higher prevalence of sexually transmitted diseases. Prevention strategies must address the wide range of gender inequalities that promote the dissemination of HIV-1. JF - Science (New York, N.Y.) AU - Quinn, Thomas C AU - Overbaugh, Julie AD - Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. tquinn@jhmi.edu Y1 - 2005/06/10/ PY - 2005 DA - 2005 Jun 10 SP - 1582 EP - 1583 VL - 308 IS - 5728 KW - Contraceptive Agents, Female KW - 0 KW - Gonadal Steroid Hormones KW - Index Medicus KW - Disease Susceptibility KW - Sexually Transmitted Diseases -- complications KW - Sex Characteristics KW - Risk Factors KW - Humans KW - Contraceptive Agents, Female -- adverse effects KW - Pregnancy Complications, Infectious -- epidemiology KW - Gonadal Steroid Hormones -- physiology KW - Sex Distribution KW - Female KW - Sexually Transmitted Diseases -- epidemiology KW - Pregnancy KW - Acquired Immunodeficiency Syndrome -- epidemiology KW - HIV Infections -- transmission KW - HIV Infections -- prevention & control KW - HIV Infections -- etiology KW - Disease Outbreaks KW - HIV-1 KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67920442?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Science+%28New+York%2C+N.Y.%29&rft.atitle=HIV%2FAIDS+in+women%3A+an+expanding+epidemic.&rft.au=Quinn%2C+Thomas+C%3BOverbaugh%2C+Julie&rft.aulast=Quinn&rft.aufirst=Thomas&rft.date=2005-06-10&rft.volume=308&rft.issue=5728&rft.spage=1582&rft.isbn=&rft.btitle=&rft.title=Science+%28New+York%2C+N.Y.%29&rft.issn=1095-9203&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-24 N1 - Date created - 2005-06-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Genetic Variation in BDNF Function Affects Human Hippocampal Structure and Function Related to Episodic Memory T2 - Seventh Annual Meeting of the International Behavioural and Neural Genetics Society (IBANGS) AN - 39649706; 3955122 JF - Seventh Annual Meeting of the International Behavioural and Neural Genetics Society (IBANGS) AU - Pezawas, Lukas Y1 - 2005/06/09/ PY - 2005 DA - 2005 Jun 09 KW - Structure-function relationships KW - Memory KW - Genetic diversity KW - Hippocampus KW - Brain-derived neurotrophic factor KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39649706?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Seventh+Annual+Meeting+of+the+International+Behavioural+and+Neural+Genetics+Society+%28IBANGS%29&rft.atitle=Genetic+Variation+in+BDNF+Function+Affects+Human+Hippocampal+Structure+and+Function+Related+to+Episodic+Memory&rft.au=Pezawas%2C+Lukas&rft.aulast=Pezawas&rft.aufirst=Lukas&rft.date=2005-06-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Seventh+Annual+Meeting+of+the+International+Behavioural+and+Neural+Genetics+Society+%28IBANGS%29&rft.issn=&rft_id=info:doi/ L2 - http://www.ibngs.org/documents/program.pdf LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - The endogenous cannabinoid anandamide and its synthetic analog R(+)-methanandamide are intravenously self-administered by squirrel monkeys. AN - 67915147; 15944392 AB - Anandamide, an endogenous ligand for brain cannabinoid CB(1) receptors, produces many behavioral effects similar to those of Delta(9)-tetrahydrocannabinol (THC), the main psychoactive ingredient in marijuana. Reinforcing effects of THC have been demonstrated in experimental animals, but there is only indirect evidence that endogenous cannabinoids such as anandamide participate in brain reward processes. We now show that anandamide serves as an effective reinforcer of drug-taking behavior when self-administered intravenously by squirrel monkeys. We also show that methanandamide, a synthetic long-lasting anandamide analog, similarly serves as a reinforcer of drug-taking behavior. Finally, we show that the reinforcing effects of both anandamide and methanandamide are blocked by pretreatment with the cannabinoid CB(1) receptor antagonist rimonabant (SR141716). These findings strongly suggest that release of endogenous cannabinoids is involved in brain reward processes and that activation of cannabinoid CB(1) receptors by anandamide could be part of the signaling of natural rewarding events. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Justinova, Zuzana AU - Solinas, Marcello AU - Tanda, Gianluigi AU - Redhi, Godfrey H AU - Goldberg, Steven R AD - Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland 21224, USA. Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 SP - 5645 EP - 5650 VL - 25 IS - 23 KW - Arachidonic Acids KW - 0 KW - Cannabinoid Receptor Modulators KW - Endocannabinoids KW - Piperidines KW - Polyunsaturated Alkamides KW - Pyrazoles KW - Receptor, Cannabinoid, CB1 KW - methanandamide KW - 150314-39-9 KW - Cocaine KW - I5Y540LHVR KW - rimonabant KW - RML78EN3XE KW - anandamide KW - UR5G69TJKH KW - Index Medicus KW - Animals KW - Stereoisomerism KW - Receptor, Cannabinoid, CB1 -- antagonists & inhibitors KW - Infusions, Intravenous KW - Substance-Related Disorders -- psychology KW - Cocaine -- administration & dosage KW - Piperidines -- pharmacology KW - Pyrazoles -- pharmacology KW - Saimiri KW - Self Administration KW - Receptor, Cannabinoid, CB1 -- physiology KW - Cocaine -- pharmacology KW - Male KW - Cannabinoid Receptor Modulators -- pharmacology KW - Cannabinoid Receptor Modulators -- administration & dosage KW - Reinforcement (Psychology) KW - Arachidonic Acids -- chemistry KW - Arachidonic Acids -- administration & dosage KW - Cannabinoid Receptor Modulators -- chemistry KW - Arachidonic Acids -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67915147?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=The+endogenous+cannabinoid+anandamide+and+its+synthetic+analog+R%28%2B%29-methanandamide+are+intravenously+self-administered+by+squirrel+monkeys.&rft.au=Justinova%2C+Zuzana%3BSolinas%2C+Marcello%3BTanda%2C+Gianluigi%3BRedhi%2C+Godfrey+H%3BGoldberg%2C+Steven+R&rft.aulast=Justinova&rft.aufirst=Zuzana&rft.date=2005-06-08&rft.volume=25&rft.issue=23&rft.spage=5645&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-17 N1 - Date created - 2005-06-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Neurochem. 2000 Apr;74(4):1627-35 [10737621] Neuropsychopharmacology. 2005 Nov;30(11):2035-45 [15812567] Psychopharmacology (Berl). 2000 Apr;149(2):121-8 [10805606] Nat Neurosci. 2000 Nov;3(11):1073-4 [11036260] J Neurochem. 2000 Dec;75(6):2434-44 [11080195] Neuropsychopharmacology. 2001 Feb;24(2):97-129 [11120394] Mol Pharmacol. 2001 Jul;60(1):155-63 [11408610] Psychopharmacology (Berl). 2001 Aug;156(4):369-80 [11498713] Neuroscience. 2001;106(1):1-4 [11564411] J Pharmacol Exp Ther. 2002 May;301(2):698-704 [11961075] J Neurosci. 2002 May 1;22(9):3326-31 [11978807] Prostaglandins Leukot Essent Fatty Acids. 2002 Feb-Mar;66(2-3):377-91 [12052051] Brain Res. 2002 Nov 1;954(1):73-81 [12393235] J Pharmacol Exp Ther. 2003 Jul;306(1):93-102 [12660305] Physiol Rev. 2003 Jul;83(3):1017-66 [12843414] Psychopharmacology (Berl). 2003 Sep;169(2):135-40 [12827345] Psychopharmacology (Berl). 2003 Sep;169(2):115-34 [12827346] Nat Rev Neurosci. 2003 Nov;4(11):873-84 [14595399] Eur J Pharmacol. 2003 Nov 7;480(1-3):133-50 [14623357] Nat Rev Neurosci. 2004 Jun;5(6):483-94 [15152198] Exp Clin Psychopharmacol. 2004 Aug;12(3):173-9 [15301634] Nat Rev Drug Discov. 2004 Sep;3(9):771-84 [15340387] Neuropharmacology. 2004;47 Suppl 1:359-67 [15464150] Psychopharmacology (Berl). 2004 Nov;176(2):223-32 [15083252] J Pharmacol Exp Ther. 1973 Jul;186(1):18-30 [4198773] Science. 1992 Dec 18;258(5090):1946-9 [1470919] J Med Chem. 1994 Jun 10;37(12):1889-93 [8021930] J Pharmacol Exp Ther. 1994 Jul;270(1):219-27 [8035318] FEBS Lett. 1994 Aug 22;350(2-3):240-4 [8070571] Life Sci. 1996;58(15):1249-57 [8614278] J Pharmacol Exp Ther. 1998 Mar;284(3):1209-17 [9495885] Life Sci. 1998;62(26):2431-9 [9651110] J Pharmacol Exp Ther. 1998 Nov;287(2):598-605 [9808686] Science. 1999 Jan 15;283(5400):401-4 [9888857] Nat Neurosci. 1999 Apr;2(4):358-63 [10204543] Nature. 1999 Jul 29;400(6743):452-7 [10440374] Behav Pharmacol. 1999 May;10(3):327-31 [10780247] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Treatment of 4 Patients With Cryopyrin-Associated Periodic Syndromes With the Long Acting IL-1 Inhibitor IL-1 Trap T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39692372; 3946390 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Canna, S AU - Gelabert, A AU - Aksentijevich, I AU - Mellis, S AU - Radin, A AU - Papadopoulos, J AU - Barham, B AU - Wilson, M AU - Hawkins, P AU - Kastner, D AU - Goldbach-Mansky, R Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - Symptoms KW - Inhibitors KW - Interleukin 1 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39692372?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=Treatment+of+4+Patients+With+Cryopyrin-Associated+Periodic+Syndromes+With+the+Long+Acting+IL-1+Inhibitor+IL-1+Trap&rft.au=Canna%2C+S%3BGelabert%2C+A%3BAksentijevich%2C+I%3BMellis%2C+S%3BRadin%2C+A%3BPapadopoulos%2C+J%3BBarham%2C+B%3BWilson%2C+M%3BHawkins%2C+P%3BKastner%2C+D%3BGoldbach-Mansky%2C+R&rft.aulast=Canna&rft.aufirst=S&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Duration of Disease and Functional Improvement in Rheumatoid Arthritis Clinical Trials: A Pooled Analysis T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39663418; 3946855 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Aletaha, D AU - Ward, M Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - Clinical trials KW - Rheumatoid arthritis KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39663418?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=Duration+of+Disease+and+Functional+Improvement+in+Rheumatoid+Arthritis+Clinical+Trials%3A+A+Pooled+Analysis&rft.au=Aletaha%2C+D%3BWard%2C+M&rft.aulast=Aletaha&rft.aufirst=D&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Environmental Factors and Muscle Weakness T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39661095; 3946979 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Miller, F W Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - Environmental factors KW - Muscles KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39661095?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=Environmental+Factors+and+Muscle+Weakness&rft.au=Miller%2C+F+W&rft.aulast=Miller&rft.aufirst=F&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Unraveling the Pathophysiology of Neonatal Onset Mutisystem Inflammatory Disease (NOMID/CINCA) By Blocking Il-1 With the Il-1 Receptor Antagonist Anakinra T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39639703; 3948010 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Goldbach-Mansky, R AU - Canna, S AU - Dailey, N AU - Gelabert, A AU - Rubin, B AU - Kim, J AU - Brewer, C AU - Butman, J AU - Turner, M AU - Karp, B AU - Hill, S AU - Aksentijevich, I AU - Haverkamp, M AU - Holland, S AU - Hoffman, S AU - Pham, T H AU - Hawkins, P AU - Kastner, D L Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - Inflammatory diseases KW - Neonates KW - Interleukin 1 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39639703?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=Unraveling+the+Pathophysiology+of+Neonatal+Onset+Mutisystem+Inflammatory+Disease+%28NOMID%2FCINCA%29+By+Blocking+Il-1+With+the+Il-1+Receptor+Antagonist+Anakinra&rft.au=Goldbach-Mansky%2C+R%3BCanna%2C+S%3BDailey%2C+N%3BGelabert%2C+A%3BRubin%2C+B%3BKim%2C+J%3BBrewer%2C+C%3BButman%2C+J%3BTurner%2C+M%3BKarp%2C+B%3BHill%2C+S%3BAksentijevich%2C+I%3BHaverkamp%2C+M%3BHolland%2C+S%3BHoffman%2C+S%3BPham%2C+T+H%3BHawkins%2C+P%3BKastner%2C+D+L&rft.aulast=Goldbach-Mansky&rft.aufirst=R&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Genetic Susceptibility to the Neonatal-Onset Multisystem Inflammatory Disease (NOMID/CINCA): Novel CIAS1 Mutations and Genomic Strategies to Resolve Locus Heterogeneity T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39613879; 3946339 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Aksentijevich, I AU - Balow, J AU - Lin, H AU - Jones, J AU - Dailey, N AU - Barham, B AU - Canna, S AU - Remmers, E F AU - Goldbach-Mansky, R AU - Kastner, D L Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - Inflammatory diseases KW - Mutation KW - Genomics KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39613879?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=Genetic+Susceptibility+to+the+Neonatal-Onset+Multisystem+Inflammatory+Disease+%28NOMID%2FCINCA%29%3A+Novel+CIAS1+Mutations+and+Genomic+Strategies+to+Resolve+Locus+Heterogeneity&rft.au=Aksentijevich%2C+I%3BBalow%2C+J%3BLin%2C+H%3BJones%2C+J%3BDailey%2C+N%3BBarham%2C+B%3BCanna%2C+S%3BRemmers%2C+E+F%3BGoldbach-Mansky%2C+R%3BKastner%2C+D+L&rft.aulast=Aksentijevich&rft.aufirst=I&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - CD4+CD25+ T Regulatory Cell Function in Rheumatoid Arthritis T2 - 6th Annual European Congress of Rheumatology (EULAR 2005) AN - 39588248; 3946567 JF - 6th Annual European Congress of Rheumatology (EULAR 2005) AU - Valencia, X AU - Vogt, S AU - Simone, J AU - Goldbach-Mansky, R AU - Lipsky, P Y1 - 2005/06/08/ PY - 2005 DA - 2005 Jun 08 KW - CD4 antigen KW - Immunoregulation KW - Rheumatoid arthritis KW - CD25 antigen KW - Lymphocytes T KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39588248?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.atitle=CD4%2BCD25%2B+T+Regulatory+Cell+Function+in+Rheumatoid+Arthritis&rft.au=Valencia%2C+X%3BVogt%2C+S%3BSimone%2C+J%3BGoldbach-Mansky%2C+R%3BLipsky%2C+P&rft.aulast=Valencia&rft.aufirst=X&rft.date=2005-06-08&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=6th+Annual+European+Congress+of+Rheumatology+%28EULAR+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.eular.org LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Energy landscape of amyloidogenic peptide oligomerization by parallel-tempering molecular dynamics simulation: significant role of Asn ladder. AN - 67911378; 15923262 AB - Recent evidence suggests that amyloidogenic oligomers may be the toxic species in cell cultures. Thus, it is crucial to understand their structure and oligomerization mechanism in atomistic detail. By employing tens of fast central processing units and an advanced phase-space sampling algorithm, parallel-tempering molecular dynamics, we have explored the energy landscape of amyloidogenic peptide oligomerization in explicit water. A pentapeptide, DFNKF, derived from human calcitonin and its mutant, DFAKF, was simulated with a total simulation time of approximately 500 ns. The detailed oligomerization process of a DFNKF parallel beta-sheet formation at 300 K has been characterized. The assembly of a parallel beta-sheet from the amorphous state mainly occurs via a "bottleneck" channel where the interstrand Asn-Asn stacking is the major interaction. The interactions of Asn-Asn stacking include both backbone and side-chain hydrogen bonds. The Asn-Asn interactions work like "glue" by sticking the DFNKF strands together and assist the "on-pathway" oligomerization. The Asn-Asn stacking observed here is similar to the Asn ladder commonly found in globular beta-helix proteins. A control run shows that when Asn is mutated to Ala, the stability and population of the DFAKF parallel beta-sheet is decreased. Furthermore, our in vitro mutagenesis experiments show that the ability of DFAKF peptides to form amyloid fibrils is significantly reduced, in agreement with the simulations. Knowledge of the energy landscape of oligomerization may provide hints for rational drug design, preventing amyloid-associated diseases. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Tsai, Hui-Hsu Gavin AU - Reches, Meital AU - Tsai, Chung-Jung AU - Gunasekaran, Kannan AU - Gazit, Ehud AU - Nussinov, Ruth AD - Basic Research Program, SAIC-Frederick, Inc., Laboratory of Experimental and Computational Biology, National Cancer Institute, Building 469, Room 145, Frederick, MD 21702, USA. tsaih@ncifcrf.gov Y1 - 2005/06/07/ PY - 2005 DA - 2005 Jun 07 SP - 8174 EP - 8179 VL - 102 IS - 23 SN - 0027-8424, 0027-8424 KW - Amyloid KW - 0 KW - Peptides KW - Asparagine KW - 7006-34-0 KW - Index Medicus KW - Protein Structure, Secondary KW - Thermodynamics KW - Humans KW - Temperature KW - Algorithms KW - Mutation -- genetics KW - Amino Acid Sequence KW - Hydrogen Bonding KW - Protein Structure, Quaternary KW - Computer Simulation KW - Amyloid -- chemistry KW - Peptides -- chemistry KW - Peptides -- genetics KW - Asparagine -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67911378?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Energy+landscape+of+amyloidogenic+peptide+oligomerization+by+parallel-tempering+molecular+dynamics+simulation%3A+significant+role+of+Asn+ladder.&rft.au=Tsai%2C+Hui-Hsu+Gavin%3BReches%2C+Meital%3BTsai%2C+Chung-Jung%3BGunasekaran%2C+Kannan%3BGazit%2C+Ehud%3BNussinov%2C+Ruth&rft.aulast=Tsai&rft.aufirst=Hui-Hsu&rft.date=2005-06-07&rft.volume=102&rft.issue=23&rft.spage=8174&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-06-08 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Biopolymers. 2003 Jan;68(1):91-109 [12579582] Structure. 2003 Mar;11(3):295-307 [12623017] Science. 2003 Apr 18;300(5618):486-9 [12702875] Science. 2003 Apr 25;300(5619):625-7 [12714741] Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5154-9 [12700355] Curr Opin Struct Biol. 2003 Apr;13(2):168-74 [12727509] Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7587-92 [12808142] Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13280-5 [14581616] Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13898-903 [14623983] Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):711-6 [14715898] Curr Opin Struct Biol. 2004 Feb;14(1):96-103 [15102455] Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8342-7 [15155909] Proteins. 2004 Aug 1;56(2):310-21 [15211514] Magn Reson Chem. 2004 Feb;42(2):247-57 [14745805] Biophys J. 2004 Jul;87(1):146-58 [15240453] Structure. 2004 Jul;12(7):1245-55 [15242601] Mol Cell Biol. 2004 Aug;24(16):7206-13 [15282319] Proteins. 2004 Nov 1;57(2):357-64 [15340923] Amyloid. 2004 Jun;11(2):81-9 [15478463] Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14760-5 [15465917] Proc Natl Acad Sci U S A. 1987 Oct;84(19):6611-5 [3477791] Biochem Biophys Res Commun. 1992 Feb 28;183(1):227-31 [1311922] J Biol Chem. 1993 Mar 25;268(9):6415-22 [8454614] J Mol Graph. 1996 Feb;14(1):33-8, 27-8 [8744570] Proteins. 1995 Dec;23(4):566-79 [8749853] Curr Opin Struct Biol. 1997 Apr;7(2):181-9 [9094324] Structure. 2002 Aug;10(8):1031-6 [12176381] J Biol Chem. 2002 Sep 20;277(38):35475-80 [12095997] J Mol Biol. 2002 Oct 4;322(5):1013-24 [12367525] Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14126-31 [12391326] Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16742-7 [12481027] Trends Biochem Sci. 1999 Sep;24(9):329-32 [10470028] J Mol Biol. 2005 Feb 4;345(5):1213-27 [15644216] Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):315-20 [15630094] Science. 2005 Jan 14;307(5707):262-5 [15653506] Science. 2002 Jul 19;297(5580):353-6 [12130773] J Mol Biol. 2000 Jan 28;295(4):1055-71 [10656810] Biochim Biophys Acta. 2000 Jul 26;1502(1):16-30 [10899428] Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11910-5 [11050225] Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2375-80 [11226247] Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):11955-60 [11572942] Prog Biophys Mol Biol. 2001 Oct;77(2):111-75 [11747907] Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):14931-6 [11752441] Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5591-5 [11960014] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The extent of chromosomal aberrations induced by chemotherapy in non-human primates depends on the schedule of administration AN - 17551683; 6393009 AB - We utilized a non-human primate model, the rhesus monkey (Macaca mulatta), to quantitate the extent of chromosomal damage in bone marrow cells following chemotherapy. Thiotepa, etoposide, and paclitaxel were chosen as the chemotherapy agents due to their distinct mechanisms of action. Chromosomal aberrations were quantitated using traditional Giemsa stain. We sought to evaluate the extent to which genotoxicity was dependent on the schedule of administration by giving chemotherapy as either a bolus or a 96h continuous infusion. Neutropenia and areas under the concentration curve (AUCs) were monitored to ensure comparable cytotoxicity and dose administered. At least 100 metaphases were scored in each marrow sample by an investigator unaware of the treatment history of the animals. All three drugs produced a statistically significant higher percentage of abnormal metaphases following bolus chemotherapy (p<0.0001, p=0.0015 and p<0.0001 for thiotepa, etoposide and paclitaxel, respectively). We conclude that infusional administration of thiotepa, etoposide and paclitaxel is less genotoxic to normal bone marrow cells than is bolus administration. These results suggest infusional regimens may be considered where there are concerns about long-term genotoxic sequelae, including secondary cancer, teratogenicity, or possibly the development of drug resistance. We believe this approach provides a reproducible model in which drugs and eventually, regimens can be compared. JF - Mutation Research-Genetic Toxicology and Environmental Mutagenesis AU - Rao, V K AU - Knutsen, T AU - Ried, T AU - Wangsa, D AU - Flynn, B M AU - Langham, G AU - Egorin, MJ AU - Cole, D AU - Balis, F AU - Steinberg, S M AU - Bates, S AU - Fojo, T AD - National Cancer Institute, NIH, Building 10, Rm 12N226, 9000 Rockville Pike, Bethesda, MD 20892, USA, tfojo@helix.nih.gov Y1 - 2005/06/06/ PY - 2005 DA - 2005 Jun 06 SP - 105 EP - 119 VL - 583 IS - 2 SN - 1383-5718, 1383-5718 KW - Rhesus monkey KW - Primates KW - Genetics Abstracts; Toxicology Abstracts KW - Drug resistance KW - Chemotherapy KW - Statistical analysis KW - Animal models KW - Bone marrow KW - Stains KW - Metaphase KW - Mutagenesis KW - Macaca mulatta KW - Drugs KW - Chromosome aberrations KW - Etoposide KW - Complications KW - Genotoxicity KW - Cancer KW - Neutropenia KW - Cytotoxicity KW - Paclitaxel KW - Teratogenicity KW - X 24117:Biochemistry KW - G 07221:Specific chemicals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17551683?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.atitle=The+extent+of+chromosomal+aberrations+induced+by+chemotherapy+in+non-human+primates+depends+on+the+schedule+of+administration&rft.au=Rao%2C+V+K%3BKnutsen%2C+T%3BRied%2C+T%3BWangsa%2C+D%3BFlynn%2C+B+M%3BLangham%2C+G%3BEgorin%2C+MJ%3BCole%2C+D%3BBalis%2C+F%3BSteinberg%2C+S+M%3BBates%2C+S%3BFojo%2C+T&rft.aulast=Rao&rft.aufirst=V&rft.date=2005-06-06&rft.volume=583&rft.issue=2&rft.spage=105&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.issn=13835718&rft_id=info:doi/10.1016%2Fj.mrgentox.2005.01.013 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Macaca mulatta; Primates; Chemotherapy; Paclitaxel; Genotoxicity; Etoposide; Chromosome aberrations; Bone marrow; Metaphase; Animal models; Complications; Stains; Neutropenia; Drugs; Statistical analysis; Drug resistance; Cancer; Teratogenicity; Cytotoxicity; Mutagenesis DO - http://dx.doi.org/10.1016/j.mrgentox.2005.01.013 ER - TY - JOUR T1 - Transmembrane inhibitors of P-glycoprotein, an ABC transporter. AN - 67876135; 15916428 AB - Drug resistance mediated by ABC transporters such as P-glycoprotein (P-gp) continues to be a major impediment to effective cancer chemotherapy. We have developed a panel of highly specific peptide inhibitors of P-gp based on the structure of the transmembrane domains of the transporter. These peptides are thought to exert their inhibitory action by disrupting the proper assembly of P-gp. A novel 96-well-plate assay based on the efflux of fluorescent P-gp substrate DiOC2 (3-ethyl-2-[3-(3-ethyl-2(3H)-benzoxazolylidene)-1-propenyl]benzoxazolium iodide) was developed and used for structure-functional characterization of transporter inhibitors. The studies strongly suggest that potent and selective inhibitors of ABC transporters can now be developed solely on the basis of the primary structures of the target proteins. The inhibition of P-gp with transmembrane peptides was shown to be chirality-independent. A 25-residue long retroinverso D-analogue of transmembrane domain 5 inhibited the efflux of the fluorescent P-gp substrate with an IC50 of 500 nM. Transmembrane peptides effectively sensitized resistant cancer cells to doxorubicin in vitro without demonstrating any cell toxicity of their own. The newly synthesized P-gp antagonists appear to be promising nontoxic drug resistance inhibitors that merit further development. JF - Journal of medicinal chemistry AU - Tarasova, Nadya I AU - Seth, Rishi AU - Tarasov, Sergey G AU - Kosakowska-Cholody, Teresa AU - Hrycyna, Christine A AU - Gottesman, Michael M AU - Michejda, Christopher J AD - Molecular Aspects of Drug Design Section, Structural Biophysics Laboratory, NCI-Frederick, P.O. Box B, Frederick, Maryland 21702, USA. tarasova@ncifcrf.gov Y1 - 2005/06/02/ PY - 2005 DA - 2005 Jun 02 SP - 3768 EP - 3775 VL - 48 IS - 11 SN - 0022-2623, 0022-2623 KW - P-Glycoprotein KW - 0 KW - Peptides KW - Index Medicus KW - Drug Screening Assays, Antitumor KW - Animals KW - Protein Structure, Secondary KW - Fluorescence KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Molecular Sequence Data KW - Mice KW - Cell Line, Tumor KW - Amino Acid Sequence KW - NIH 3T3 Cells KW - Structure-Activity Relationship KW - Peptides -- chemical synthesis KW - Peptides -- chemistry KW - P-Glycoprotein -- chemistry KW - Peptides -- pharmacology KW - P-Glycoprotein -- antagonists & inhibitors KW - Drug Resistance, Neoplasm -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67876135?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medicinal+chemistry&rft.atitle=Transmembrane+inhibitors+of+P-glycoprotein%2C+an+ABC+transporter.&rft.au=Tarasova%2C+Nadya+I%3BSeth%2C+Rishi%3BTarasov%2C+Sergey+G%3BKosakowska-Cholody%2C+Teresa%3BHrycyna%2C+Christine+A%3BGottesman%2C+Michael+M%3BMichejda%2C+Christopher+J&rft.aulast=Tarasova&rft.aufirst=Nadya&rft.date=2005-06-02&rft.volume=48&rft.issue=11&rft.spage=3768&rft.isbn=&rft.btitle=&rft.title=Journal+of+medicinal+chemistry&rft.issn=00222623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-06 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dopamine D3 receptor partial agonists and antagonists as potential drug abuse therapeutic agents. AN - 67875136; 15916415 JF - Journal of medicinal chemistry AU - Newman, Amy Hauck AU - Grundt, Peter AU - Nader, Michael A AD - National Institute on Drug Abuse-Intramural Research Program, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland 21224, USA. anewman@intra.nida.nih.gov Y1 - 2005/06/02/ PY - 2005 DA - 2005 Jun 02 SP - 3663 EP - 3679 VL - 48 IS - 11 SN - 0022-2623, 0022-2623 KW - DRD3 protein, human KW - 0 KW - Dopamine Agonists KW - Dopamine Antagonists KW - Dopamine D2 Receptor Antagonists KW - Receptors, Dopamine D2 KW - Receptors, Dopamine D3 KW - Index Medicus KW - Animals KW - Humans KW - Structure-Activity Relationship KW - Biological Availability KW - Dopamine Agonists -- therapeutic use KW - Dopamine Antagonists -- therapeutic use KW - Dopamine Agonists -- pharmacology KW - Dopamine Agonists -- pharmacokinetics KW - Dopamine Antagonists -- pharmacology KW - Receptors, Dopamine D2 -- agonists KW - Substance-Related Disorders -- drug therapy KW - Dopamine Antagonists -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67875136?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+medicinal+chemistry&rft.atitle=Dopamine+D3+receptor+partial+agonists+and+antagonists+as+potential+drug+abuse+therapeutic+agents.&rft.au=Newman%2C+Amy+Hauck%3BGrundt%2C+Peter%3BNader%2C+Michael+A&rft.aulast=Newman&rft.aufirst=Amy&rft.date=2005-06-02&rft.volume=48&rft.issue=11&rft.spage=3663&rft.isbn=&rft.btitle=&rft.title=Journal+of+medicinal+chemistry&rft.issn=00222623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-06 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Rituximab for Steroid-Refractory Autoimmune Hemolytic Anemia Associated with Chronic Lymphocytic Leukemia T2 - 10th Congress of the European Hematology Association (EHA 2005) AN - 39674263; 3951491 JF - 10th Congress of the European Hematology Association (EHA 2005) AU - D'Arena, G AU - Laurenti, L AU - Ferrara, F AU - Capalbo, S AU - Storti, S AU - Marcacci, G AU - Tarnani, M AU - Copia, C AU - Vigliotti, M L AU - Petrilli, M P AU - Filippi, R De AU - Russo, F AU - Pinto, A Y1 - 2005/06/02/ PY - 2005 DA - 2005 Jun 02 KW - Autoimmune hemolytic anemia KW - Chronic lymphatic leukemia KW - Anemia KW - Rituximab KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39674263?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=10th+Congress+of+the+European+Hematology+Association+%28EHA+2005%29&rft.atitle=Rituximab+for+Steroid-Refractory+Autoimmune+Hemolytic+Anemia+Associated+with+Chronic+Lymphocytic+Leukemia&rft.au=D%27Arena%2C+G%3BLaurenti%2C+L%3BFerrara%2C+F%3BCapalbo%2C+S%3BStorti%2C+S%3BMarcacci%2C+G%3BTarnani%2C+M%3BCopia%2C+C%3BVigliotti%2C+M+L%3BPetrilli%2C+M+P%3BFilippi%2C+R+De%3BRusso%2C+F%3BPinto%2C+A&rft.aulast=D%27Arena&rft.aufirst=G&rft.date=2005-06-02&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=10th+Congress+of+the+European+Hematology+Association+%28EHA+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.parthen-impact.com/pco/6_05EHA/public/index.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - A cancer nanotechnology strategy AN - 954582584; 13860481 JF - NanoBiotechnology AU - Ferrari, Mauro AU - Barker, Anna D AU - Downing, Gregory J AD - Office of Technology and Industrial Relations, The National Cancer Institute, National Institutes of Health, Building 31, Room 10A-52, MSC 2580, 31 Center Drive, 20892-2580, Bethesda, MD, downingg@mail.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 129 EP - 131 PB - Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA VL - 1 IS - 2 SN - 1551-1286, 1551-1286 KW - Biotechnology and Bioengineering Abstracts UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/954582584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NanoBiotechnology&rft.atitle=A+cancer+nanotechnology+strategy&rft.au=Ferrari%2C+Mauro%3BBarker%2C+Anna+D%3BDowning%2C+Gregory+J&rft.aulast=Ferrari&rft.aufirst=Mauro&rft.date=2005-06-01&rft.volume=1&rft.issue=2&rft.spage=129&rft.isbn=&rft.btitle=&rft.title=NanoBiotechnology&rft.issn=15511286&rft_id=info:doi/10.1385%2FNBT%3A1%3A2%3A129 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2012-03-01 N1 - Last updated - 2012-03-30 DO - http://dx.doi.org/10.1385/NBT:1:2:129 ER - TY - JOUR T1 - Regional cerebral blood flow in children with ADHD: changes with age. AN - 85383531; pmid-15862191 AB - The aim of this study was to investigate the changes in regional cerebral blood flow (rCBF) with age in patients with attention deficit hyperactivity disorder (ADHD). Twenty-nine drug-naive ADHD subjects (24 boys, 5 girls; age 7-13; mean+/-SD=age 9.2+/-2.1) and 12 subjects with epilepsy (all diagnosed as having complex partial seizure, 6 boys, 6 girls; age 7-14; mean+/-SD=8.5+/-2.1) were included in the study. All cases of ADHD were diagnosed according to DSM-IV criteria. Cerebral blood flow was evaluated with Tc-99m-hexamethylpropyleneamine oxime (Tc99m HMPAO) brain single photon emission tomography (SPECT) during standard resting condition in all of the cases. Asymmetry indices for each region of interest were calculated. Absolute rCBF values were normalized as the absolute rCBF values divided by the whole brain absolute value. The prefrontal lobe asymmetry indices were significantly negatively correlated with age in ADHD cases (r=-0.408, P=0.025), which indicated the increased prefrontal rCBF lateralization from the right to the left side with age. When ADHD cases older than 7 years of age were compared with those with epilepsy, the ADHD cases had lower right prefrontal and frontal rCBF and higher left parietal rCBF. The epilepsy group showed no significant correlations between age and asymmetry indices and showed a different developmental trajectory for prefrontal asymmetry and right prefrontal rCBF values. The results indicated that the left hemisphere dominance in the prefrontal cortex significantly increases with age in ADHD cases. JF - Brain & development AU - Oner, Ozgür AU - Oner, Pinar AU - Aysev, Ayla AU - Küçük, Ozlem AU - Ibis, Erkan AD - NIMH Fogarty International Mental Health and Developmental Disabilities Program, 34742 Istanbul, Turkey. ozz_oner@yahoo.com Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 279 EP - 285 VL - 27 IS - 4 SN - 0387-7604, 0387-7604 KW - Index Medicus KW - National Library of Medicine KW - Adolescent KW - Age Factors KW - *Attention Deficit Disorder with Hyperactivity: physiopathology KW - *Brain: blood supply KW - *Cerebrovascular Circulation: physiology KW - Child KW - Epilepsy: physiopathology KW - Female KW - Humans KW - Male KW - Tomography, Emission-Computed, Single-Photon UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85383531?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+%26+development&rft.atitle=Regional+cerebral+blood+flow+in+children+with+ADHD%3A+changes+with+age.&rft.au=Oner%2C+Ozg%C3%BCr%3BOner%2C+Pinar%3BAysev%2C+Ayla%3BK%C3%BC%C3%A7%C3%BCk%2C+Ozlem%3BIbis%2C+Erkan&rft.aulast=Oner&rft.aufirst=Ozg%C3%BCr&rft.date=2005-06-01&rft.volume=27&rft.issue=4&rft.spage=279&rft.isbn=&rft.btitle=&rft.title=Brain+%26+development&rft.issn=03877604&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - A randomized study of accelerated fractionation radiotherapy with and without mitomycin C in the treatment of locally advanced head and neck cancer. AN - 70148023; 16508679 AB - This single-institution study evaluates the feasibility of accelerated fractionation radiotherapy (AF) with and without mitomycin C (MMC) in the treatment of locally advanced head and neck cancer. Between May 1998 and October 2001, sixty patients with locally advanced stage III and IV of head and neck cancer were randomized into three treatment arms: (1) conventional fractionation radiotherapy (CF) (5 fractions per week); (2) accelerated fractionation radiotherapy (AF) (6 fractions per week); and (3) AF plus Mitomycin C (MMC). The 2-year overall survival (OS) of the whole group was 21%. The OS according to treatment arm was 23%, 20%, and 28% in CF, AF, and AF+MMC arms respectively (p<0.19). The 2-year loco-regional control (LC) rate was 22% for the whole group of patients. The LC was 10%, 25%, and 30% for the CF, AF, and AF+MMC respectively (p=0.27). The only significant parameters for OS and LC were performance status and pre-treatment hemoglobin level. Mucositis grades 3 & 4 occurred in 70% and 90% of the patients in the AF and AF+MMC arm respectively compared to 55% of patients in the CF arm (p=0.04). However the addition of MMC did not significantly increase the incidence or severity of mucositis between AF and AF+MMC (p=0.13). Hematological toxicity grades 3 & 4 were significantly higher after MMC (occurred in 40% of patients versus 10% and 5% in CF and AF arms respectively, p=0.04). There was no statistically significant difference in the incidence of grade 3 dryness of mouth (p=0.06), fibrosis (p=0.6), or lymphoedema (p=0.39) among the three arms. There was a trend for improvement of LC and OS rates with the use of AF and the addition of MMC to AF compared to CF radiotherapy, although the difference was not statistically significant. The small number of the patients in each treatment arm and the inclusion of multiple tumor sites may contribute to these statistically insignificant results. Accordingly we advise 85 to continue the trial with inclusion of a larger number of patients and restrict tumor sites to one major site. JF - Journal of the Egyptian National Cancer Institute AU - Ezzat, Mohamad AU - Shouman, Tarek AU - Zaza, Khaled AU - Safwat, Akmal AU - El-Khoudary, Ahmad AU - El-Senosi, Mohamad AU - Ezzat, Ibrahim AD - The Department of Radiation Oncology, National Cancer Institute, Cairo University. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 85 EP - 92 VL - 17 IS - 2 SN - 1110-0362, 1110-0362 KW - Antibiotics, Antineoplastic KW - 0 KW - Mitomycin KW - 50SG953SK6 KW - Index Medicus KW - Survival Rate KW - Combined Modality Therapy KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Adolescent KW - Radiation Injuries KW - Male KW - Female KW - Mitomycin -- adverse effects KW - Dose Fractionation KW - Carcinoma, Squamous Cell -- mortality KW - Mitomycin -- therapeutic use KW - Head and Neck Neoplasms -- radiotherapy KW - Head and Neck Neoplasms -- mortality KW - Head and Neck Neoplasms -- drug therapy KW - Carcinoma, Squamous Cell -- radiotherapy KW - Carcinoma, Squamous Cell -- drug therapy KW - Antibiotics, Antineoplastic -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70148023?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Egyptian+National+Cancer+Institute&rft.atitle=A+randomized+study+of+accelerated+fractionation+radiotherapy+with+and+without+mitomycin+C+in+the+treatment+of+locally+advanced+head+and+neck+cancer.&rft.au=Ezzat%2C+Mohamad%3BShouman%2C+Tarek%3BZaza%2C+Khaled%3BSafwat%2C+Akmal%3BEl-Khoudary%2C+Ahmad%3BEl-Senosi%2C+Mohamad%3BEzzat%2C+Ibrahim&rft.aulast=Ezzat&rft.aufirst=Mohamad&rft.date=2005-06-01&rft.volume=17&rft.issue=2&rft.spage=85&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Egyptian+National+Cancer+Institute&rft.issn=11100362&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-14 N1 - Date created - 2006-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Liquid Tissue: proteomic profiling of formalin-fixed tissues. AN - 70142313; 16528915 AB - Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue MS protein preparation allows researchers to utilize the vast, already existing, collections offormalin-fixed paraffin-embedded (FFPE) tissues for the procurement of peptides and the analysis across a variety of MS platforms. JF - BioTechniques AU - Prieto, DaRue A AU - Hood, Brian L AU - Darfler, Marlene M AU - Guiel, Thomas G AU - Lucas, David A AU - Conrads, Thomas P AU - Veenstra, Timothy D AU - Krizman, David B AD - SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 32 EP - 35 VL - Suppl SN - 0736-6205, 0736-6205 KW - Neoplasm Proteins KW - 0 KW - Formaldehyde KW - 1HG84L3525 KW - Index Medicus KW - Mass Spectrometry KW - Paraffin Embedding KW - Humans KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization KW - Chromatography, Liquid KW - Formaldehyde -- chemistry KW - Proteomics KW - Colonic Neoplasms -- chemistry KW - Neoplasm Proteins -- analysis KW - Colonic Neoplasms -- pathology KW - Tissue Fixation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70142313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BioTechniques&rft.atitle=Liquid+Tissue%3A+proteomic+profiling+of+formalin-fixed+tissues.&rft.au=Prieto%2C+DaRue+A%3BHood%2C+Brian+L%3BDarfler%2C+Marlene+M%3BGuiel%2C+Thomas+G%3BLucas%2C+David+A%3BConrads%2C+Thomas+P%3BVeenstra%2C+Timothy+D%3BKrizman%2C+David+B&rft.aulast=Prieto&rft.aufirst=DaRue&rft.date=2005-06-01&rft.volume=Suppl&rft.issue=&rft.spage=32&rft.isbn=&rft.btitle=&rft.title=BioTechniques&rft.issn=07366205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-04-06 N1 - Date created - 2006-03-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Molecular dissection of purinergic P2X receptor channels. AN - 68567953; 16154926 AB - The P2X receptors (P2XRs) are a family of ATP-gated channels expressed in the plasma membrane of numerous excitable and nonexcitable cells and play important roles in control of cellular functions, such as neurotransmission, hormone secretion, transcriptional regulation, and protein synthesis. P2XRs are homomeric or heteromeric proteins, formed by assembly of at least three of seven subunits named P2X(1)-P2X(7). All subunits possess intracellular N- and C-termini, two transmembrane domains, and a relatively large extracellular ligand-binding loop. ATP binds to still an unidentified extracellular domain, leading to a sequence of conformational transitions between closed, open, and desensitized states. Removal of extracellular ATP leads to deactivation and resensitization of receptors. Activated P2XRs generate inward currents caused by Na(+) and Ca(2+) influx through the pore of channels, and thus mediate membrane depolarization and facilitation of voltage-gated calcium entry in excitable cells. No crystal structures are available for P2XRs and these receptors have no obvious similarity to other ion channels or ATP binding proteins, which limits the progress in understanding the relationship between molecular structure and conformational transitions of receptor in the presence of agonist and after its washout. We summarize here the alternative approaches in studies on molecular properties of P2XRs, including heteromerization, chimerization, mutagenesis, and biochemical studies. JF - Annals of the New York Academy of Sciences AU - Stojilkovic, Stanko S AU - Tomic, Melanija AU - He, Mu-Lan AU - Yan, Zonghe AU - Koshimizu, Taka-Aki AU - Zemkova, Hana AD - Section on Cellular Signaling, Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. stankos@helix.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 116 EP - 130 VL - 1048 SN - 0077-8923, 0077-8923 KW - Ion Channels KW - 0 KW - Ligands KW - Purinergic P2 Receptor Agonists KW - Receptors, Purinergic P2 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Index Medicus KW - Animals KW - Membrane Potentials -- physiology KW - Amino Acid Sequence KW - Electrophysiology KW - Structure-Activity Relationship KW - Binding Sites KW - Chimera KW - Cells, Cultured KW - Extracellular Space -- metabolism KW - Adenosine Triphosphate -- metabolism KW - Molecular Sequence Data KW - Time Factors KW - Mutation KW - Receptors, Purinergic P2 -- genetics KW - Ion Channel Gating -- physiology KW - Ion Channels -- chemistry KW - Receptors, Purinergic P2 -- metabolism KW - Ion Channels -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68567953?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Molecular+dissection+of+purinergic+P2X+receptor+channels.&rft.au=Stojilkovic%2C+Stanko+S%3BTomic%2C+Melanija%3BHe%2C+Mu-Lan%3BYan%2C+Zonghe%3BKoshimizu%2C+Taka-Aki%3BZemkova%2C+Hana&rft.aulast=Stojilkovic&rft.aufirst=Stanko&rft.date=2005-06-01&rft.volume=1048&rft.issue=&rft.spage=116&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-07-19 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microglia and inflammation-mediated neurodegeneration: multiple triggers with a common mechanism. AN - 68559147; 16081203 AB - Inflammation, a common denominator among the diverse list of neurodegenerative diseases, has recently been implicated as a critical mechanism responsible for the progressive nature of neurodegeneration. Microglia are the resident innate immune cells in the central nervous system and produce a barrage of factors (IL-1, TNFalpha, NO, PGE2, superoxide) that are toxic to neurons. Evidence supports that the unregulated activation of microglia in response to environmental toxins, endogenous proteins, and neuronal death results in the production of toxic factors that propagate neuronal injury. In the following review, we discuss the common thread of microglial activation across numerous neurodegenerative diseases, define current perceptions of how microglia are damaging neurons, and explain how the microglial response to neuronal damage results in a self-propelling cycle of neuron death. JF - Progress in neurobiology AU - Block, Michelle L AU - Hong, Jau-Shyong AD - Neuropharmacology Section, MD F1-01, National Institute of Environmental Health Sciences, P.O. Box 12233, Research Triangle Park, NC 27709, USA. block@niehs.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 77 EP - 98 VL - 76 IS - 2 SN - 0301-0082, 0301-0082 KW - Amyloid beta-Peptides KW - 0 KW - Toxins, Biological KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Animals KW - Toxins, Biological -- toxicity KW - Amyloid beta-Peptides -- metabolism KW - Humans KW - Dopamine -- metabolism KW - Models, Biological KW - Neurodegenerative Diseases -- classification KW - Neurodegenerative Diseases -- metabolism KW - Neurodegenerative Diseases -- pathology KW - Microglia -- classification KW - Inflammation -- metabolism KW - Microglia -- pathology KW - Microglia -- drug effects KW - Microglia -- metabolism KW - Inflammation -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68559147?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+neurobiology&rft.atitle=Microglia+and+inflammation-mediated+neurodegeneration%3A+multiple+triggers+with+a+common+mechanism.&rft.au=Block%2C+Michelle+L%3BHong%2C+Jau-Shyong&rft.aulast=Block&rft.aufirst=Michelle&rft.date=2005-06-01&rft.volume=76&rft.issue=2&rft.spage=77&rft.isbn=&rft.btitle=&rft.title=Progress+in+neurobiology&rft.issn=03010082&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-15 N1 - Date created - 2005-09-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Risk factors to pesticide exposure and associated health symptoms among cut-flower farmers. AN - 68537523; 16134479 AB - The study looked into the risk factors associated with pesticide exposure among cut-flower farmers. A survey questionnaire was given to 102 respondents in Barangay Bahong in La Trinidad, the center of cut-flower production in the Philippines. Results showed that 32% were symptomatic or had experienced pesticide-related illnesses since their first use of pesticides. The majority of the pesticides used by the farmers were Categories Ib and II which are moderately or highly hazardous chemicals. Individuals with signs and symptoms most often centered on the eye, ear, nose and throat (EENT) (44 respondents reporting these symptoms) followed by general and neuralgic (16 respondents) and the integumentary (14 respondents). The most common general signs and symptoms manifested were weakness followed by fatigue and muscle pain then by chills and fever. The most common EENT manifestations were eye itchiness and blurring of vision. For neurological signs and symptoms, dizziness followed by headache was reported. Logistic regression showed that illnesses for the past 12 months were associated with certain risk factors such as farm use of pesticides, exposure to pesticide while applying it, respiratory inhalation of pesticide vapours and mists (p = 0.05). Moreover, those who re-entered a recently sprayed area were 20 times more likely to get ill during the past 12 months than those who did not. Those who used pesticide-contaminated pieces of fabric to wipe sweat off their faces were 2% more likely to get ill, and those who had spills on their bodies while applying pesticide were 26 times more likely to get ill. The study suggested that the risk factors to pesticide exposure should be considered in policy formulations for the cut-flower farmers in the country. JF - International journal of environmental health research AU - Lu, Jinky Leilanie AD - University of the Philippines, National Institutes of Health, and Affiliate, College of Arts and Sciences, Manila, The Philippines. jinky_lu@yahoo.com Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 161 EP - 169 VL - 15 IS - 3 SN - 0960-3123, 0960-3123 KW - Pesticides KW - 0 KW - Index Medicus KW - Agriculture KW - Philippines KW - Fatigue KW - Humans KW - Fever -- etiology KW - Pain -- etiology KW - Risk Factors KW - Adult KW - Health Surveys KW - Headache -- etiology KW - Adolescent KW - Female KW - Male KW - Occupational Exposure KW - Occupational Health KW - Flowers KW - Pesticides -- poisoning UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68537523?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+environmental+health+research&rft.atitle=Risk+factors+to+pesticide+exposure+and+associated+health+symptoms+among+cut-flower+farmers.&rft.au=Lu%2C+Jinky+Leilanie&rft.aulast=Lu&rft.aufirst=Jinky&rft.date=2005-06-01&rft.volume=15&rft.issue=3&rft.spage=161&rft.isbn=&rft.btitle=&rft.title=International+journal+of+environmental+health+research&rft.issn=09603123&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-09-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In vivo biological activity of exendin (1-30). AN - 68443438; 16077164 AB - Activation of the glucagon-like peptide-1 (GLP-1) receptor on pancreatic beta cells by GLP-1 and exendin-4 increases insulin secretion. Exendin-4 is 39 amino acids long, unlike GLP-1 which has 30 amino acids. Because of its non-mammalian (lizard) origin and unique C-terminal sequence, exendin-4 may be immunogenic in humans. We showed previously that the C terminally truncated exendin peptide exendin (1-30) has a reduced affinity for the GLP-1 receptor and a diminished ability to increase intracellular cAMP in insulinoma cells. Here we show that daily intraperitoneal injection of exendin (1-30) (1 nmol/kg) for 20 d followed by 31 d twice daily to Lepr(db)/Lepr(db) (db/db) mice significantly reduced the amount of visceral fat relative to saline-treated controls and improved HbA1C (control 9.5 +/- 0.2% vs treated 7.9 +/- 0.2%, p = 0.001) but was not as effective as exendin-4. To examine the ability of exendin (1-30) to stimulate beta-cell growth, we injected one group of 3-mo-old Fisher rats with exendin (1-30) (1 nmol/kg) and another group with saline for 8 d. We observed no change in beta-cell area, but did see a change in the number of islets with nuclei positive for BrdU [10.7 +/- 1.8% exendin (1-30) vs 6.5 +/- 0.5% control]. JF - Endocrine AU - Doyle, Máire E AU - McConville, Patrick AU - Theodorakis, Michael J AU - Goetschkes, Margaret M AU - Bernier, Michel AU - Spencer, Richard G S AU - Holloway, Harold W AU - Greig, Nigel H AU - Egan, Josephine M AD - Diabetes Section National Institute on Aging, National Institutes of Health. Diabetes Section, National Institute on Aging, National Institutes of Health, Baltimore MD 21224, USA. medoyle@dental.ufl.edu Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1 EP - 9 VL - 27 IS - 1 SN - 1355-008X, 1355-008X KW - Blood Glucose KW - 0 KW - Hemoglobin A, Glycosylated KW - Insulin KW - Peptides KW - Venoms KW - exenatide KW - 9P1872D4OL KW - Bromodeoxyuridine KW - G34N38R2N1 KW - Index Medicus KW - Microscopy, Confocal KW - Magnetic Resonance Imaging KW - Injections, Intraperitoneal KW - Cell Proliferation -- drug effects KW - Eating -- drug effects KW - Animals KW - Blood Glucose -- metabolism KW - Islets of Langerhans -- drug effects KW - Insulin -- metabolism KW - Diabetes Mellitus -- genetics KW - Mice KW - Amino Acid Sequence KW - Rats KW - Mice, Inbred Strains KW - Rats, Inbred F344 KW - Hemoglobin A, Glycosylated -- metabolism KW - Diabetes Mellitus -- blood KW - Molecular Sequence Data KW - Mice, Inbred C57BL KW - Adipose Tissue -- drug effects KW - Body Composition -- drug effects KW - Immunoenzyme Techniques KW - Bromodeoxyuridine -- metabolism KW - Weight Gain -- drug effects KW - Peptides -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68443438?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Endocrine&rft.atitle=In+vivo+biological+activity+of+exendin+%281-30%29.&rft.au=Doyle%2C+M%C3%A1ire+E%3BMcConville%2C+Patrick%3BTheodorakis%2C+Michael+J%3BGoetschkes%2C+Margaret+M%3BBernier%2C+Michel%3BSpencer%2C+Richard+G+S%3BHolloway%2C+Harold+W%3BGreig%2C+Nigel+H%3BEgan%2C+Josephine+M&rft.aulast=Doyle&rft.aufirst=M%C3%A1ire&rft.date=2005-06-01&rft.volume=27&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Endocrine&rft.issn=1355008X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-08-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nicotinic agonists, antagonists, and modulators from natural sources. AN - 68439642; 16075378 AB - 1. Acetylcholine receptors were initially defined as nicotinic or muscarinic, based on selective activation by two natural products, nicotine and muscarine. Several further nicotinic agonists have been discovered from natural sources, including cytisine, anatoxin, ferruginine, anabaseine, epibatidine, and epiquinamide. These have provided lead structures for the design of a wide range of synthetic agents. 2. Natural sources have also provided competitive nicotinic antagonists, such as the Erythrina alkaloids, the tubocurarines, and methyllycaconitine. Noncompetitive antagonists, such as the histrionicotoxins, various izidines, decahydroquinolines, spiropyrrolizidine oximes, pseudophrynamines, ibogaine, strychnine, cocaine, and sparteine have come from natural sources. Finally, galanthamine, codeine, and ivermectin represent positive modulators of nicotinic function, derived from natural sources. 3. Clearly, research on acetylcholine receptors and functions has been dependent on key natural products and the synthetic agents that they inspired. JF - Cellular and molecular neurobiology AU - Daly, John W AD - Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, DHHS, Bethesda, Maryland 20892, USA. jdaly@nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 513 EP - 552 VL - 25 IS - 3-4 SN - 0272-4340, 0272-4340 KW - Nicotinic Agonists KW - 0 KW - Nicotinic Antagonists KW - Toxins, Biological KW - Venoms KW - Index Medicus KW - Animals KW - Humans KW - Plants -- chemistry KW - Nicotinic Antagonists -- isolation & purification KW - Nicotinic Agonists -- isolation & purification KW - Nicotinic Antagonists -- chemistry KW - Toxins, Biological -- isolation & purification KW - Toxins, Biological -- chemistry KW - Venoms -- isolation & purification KW - Venoms -- chemistry KW - Nicotinic Agonists -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68439642?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cellular+and+molecular+neurobiology&rft.atitle=Nicotinic+agonists%2C+antagonists%2C+and+modulators+from+natural+sources.&rft.au=Daly%2C+John+W&rft.aulast=Daly&rft.aufirst=John&rft.date=2005-06-01&rft.volume=25&rft.issue=3-4&rft.spage=513&rft.isbn=&rft.btitle=&rft.title=Cellular+and+molecular+neurobiology&rft.issn=02724340&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-08-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lung cancer after Hodgkin lymphoma: the roles of chemotherapy, radiotherapy and tobacco use. AN - 68057990; 16044495 JF - Radiation research AU - Travis, L B AU - Gilbert, E AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute (NCI), National Institutes of Health, Bethesda, Maryland, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 695 EP - 696 VL - 163 IS - 6 SN - 0033-7587, 0033-7587 KW - Index Medicus KW - Space life sciences KW - Causality KW - Radiation Protection -- methods KW - Risk Factors KW - Humans KW - Clinical Trials as Topic KW - United States -- epidemiology KW - Comorbidity KW - Prevalence KW - Lung Neoplasms -- epidemiology KW - Neoplasms, Radiation-Induced -- epidemiology KW - Drug Therapy -- statistics & numerical data KW - Risk Assessment -- methods KW - Hodgkin Disease -- therapy KW - Smoking -- epidemiology KW - Radiotherapy -- statistics & numerical data KW - Hodgkin Disease -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057990?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+research&rft.atitle=Lung+cancer+after+Hodgkin+lymphoma%3A+the+roles+of+chemotherapy%2C+radiotherapy+and+tobacco+use.&rft.au=Travis%2C+L+B%3BGilbert%2C+E&rft.aulast=Travis&rft.aufirst=L&rft.date=2005-06-01&rft.volume=163&rft.issue=6&rft.spage=695&rft.isbn=&rft.btitle=&rft.title=Radiation+research&rft.issn=00337587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-11 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene environment interactions in a cohort of irradiated retinoblastoma patients. AN - 68057852; 16044502 JF - Radiation research AU - Kleinerman, R A AU - Stovall, M AU - Tarone, R E AU - Tucker, M A AD - Division of Cancer, Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland 20852, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 701 EP - 702 VL - 163 IS - 6 SN - 0033-7587, 0033-7587 KW - Index Medicus KW - Space life sciences KW - Risk Factors KW - Humans KW - Cohort Studies KW - Genetic Predisposition to Disease -- epidemiology KW - United States -- epidemiology KW - Sex Distribution KW - Genes, Retinoblastoma -- genetics KW - Prevalence KW - Age Distribution KW - Retinal Neoplasms -- genetics KW - Neoplasms, Radiation-Induced -- epidemiology KW - Retinoblastoma -- genetics KW - Retinal Neoplasms -- radiotherapy KW - Retinoblastoma -- radiotherapy KW - Neoplasms, Radiation-Induced -- genetics KW - Risk Assessment -- methods KW - Retinal Neoplasms -- epidemiology KW - Retinoblastoma -- epidemiology KW - Radiotherapy -- statistics & numerical data UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68057852?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+research&rft.atitle=Gene+environment+interactions+in+a+cohort+of+irradiated+retinoblastoma+patients.&rft.au=Kleinerman%2C+R+A%3BStovall%2C+M%3BTarone%2C+R+E%3BTucker%2C+M+A&rft.aulast=Kleinerman&rft.aufirst=R&rft.date=2005-06-01&rft.volume=163&rft.issue=6&rft.spage=701&rft.isbn=&rft.btitle=&rft.title=Radiation+research&rft.issn=00337587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-11 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Second cancers after radiotherapy: any evidence for radiation-induced genomic instability? AN - 68056798; 16044503 JF - Radiation research AU - Sigurdson, Alice J AU - Jones, Irene M AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, NCI, NIH, DHHS, Bethesda, Maryland, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 702 EP - 703 VL - 163 IS - 6 SN - 0033-7587, 0033-7587 KW - Index Medicus KW - Space life sciences KW - Neoplasms -- complications KW - Neoplasms -- radiotherapy KW - Risk Factors KW - Humans KW - Clinical Trials as Topic KW - Neoplasms -- genetics KW - Genomic Instability -- radiation effects KW - Neoplasms, Radiation-Induced -- etiology KW - DNA Damage KW - Evidence-Based Medicine -- methods KW - Genomic Instability -- genetics KW - Neoplasms, Radiation-Induced -- genetics KW - Risk Assessment -- methods KW - Neoplasms, Radiation-Induced -- secondary KW - Radiotherapy -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68056798?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+research&rft.atitle=Second+cancers+after+radiotherapy%3A+any+evidence+for+radiation-induced+genomic+instability%3F&rft.au=Sigurdson%2C+Alice+J%3BJones%2C+Irene+M&rft.aulast=Sigurdson&rft.aufirst=Alice&rft.date=2005-06-01&rft.volume=163&rft.issue=6&rft.spage=702&rft.isbn=&rft.btitle=&rft.title=Radiation+research&rft.issn=00337587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-11 N1 - Date created - 2005-07-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selective dopamine D3 receptor antagonism by SB-277011A attenuates cocaine reinforcement as assessed by progressive-ratio and variable-cost-variable-payoff fixed-ratio cocaine self-administration in rats. AN - 68053975; 16026480 AB - In rats, acute administration of SB-277011A, a highly selective dopamine (DA) D(3) receptor antagonist, blocks cocaine-enhanced brain stimulation reward, cocaine-seeking behaviour and reinstatement of cocaine-seeking behaviour. Here, we investigated whether SB-277011A attenuates cocaine reinforcement as assessed by cocaine self-administration under variable-cost-variable-payoff fixed-ratio (FR) and progressive-ratio (PR) reinforcement schedules. Acute i.p. administration of SB-277011A (3-24 mg/kg) did not significantly alter cocaine (0.75 mg/kg/infusion) self-administration reinforced under FR1 (one lever press for one cocaine infusion) conditions. However, acute administration of SB-277011A (24 mg/kg, i.p.) progressively attenuated cocaine self-administration when: (a) the unit dose of self-administered cocaine was lowered from 0.75 to 0.125-0.5 mg/kg, and (b) the work demand for cocaine reinforcement was increased from FR1 to FR10. Under PR (increasing number of lever presses for each successive cocaine infusion) cocaine reinforcement, acute administration of SB-277011A (6-24 mg/kg i.p.) lowered the PR break point for cocaine self-administration in a dose-dependent manner. The reduction in the cocaine (0.25-1.0 mg/kg) dose-response break-point curve produced by 24 mg/kg SB-277011A is consistent with a reduction in cocaine's reinforcing efficacy. When substituted for cocaine, SB-277011A alone did not sustain self-administration behaviour. In contrast with the mixed DA D(2)/D(3) receptor antagonist haloperidol (1 mg/kg), SB-277011A (3, 12 or 24 mg/kg) failed to impede locomotor activity, failed to impair rearing behaviour, failed to produce catalepsy and failed to impair rotarod performance. These results show that SB-277011A significantly inhibits acute cocaine-induced reinforcement except at high cocaine doses and low work requirement for cocaine. If these results extrapolate to humans, SB-277011A or similar selective DA D(3) receptor antagonists may be useful in the treatment of cocaine addiction. JF - The European journal of neuroscience AU - Xi, Zheng-Xiong AU - Gilbert, Jeremy G AU - Pak, Arlene C AU - Ashby, Charles R AU - Heidbreder, Christian A AU - Gardner, Eliot L AD - Neuropsychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 3427 EP - 3438 VL - 21 IS - 12 SN - 0953-816X, 0953-816X KW - Dopamine Antagonists KW - 0 KW - Dopamine Uptake Inhibitors KW - Nitriles KW - SB 277011 KW - Tetrahydroisoquinolines KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Rats KW - Conditioning, Operant -- drug effects KW - Behavior, Animal -- drug effects KW - Animals KW - Drug Interactions KW - Self Administration KW - Reinforcement Schedule KW - Rats, Long-Evans KW - Dose-Response Relationship, Drug KW - Conditioning, Operant -- physiology KW - Time Factors KW - Male KW - Tetrahydroisoquinolines -- therapeutic use KW - Dopamine Antagonists -- therapeutic use KW - Dopamine Uptake Inhibitors -- administration & dosage KW - Reinforcement (Psychology) KW - Cocaine-Related Disorders -- drug therapy KW - Cocaine-Related Disorders -- physiopathology KW - Cocaine-Related Disorders -- etiology KW - Nitriles -- therapeutic use KW - Cocaine -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68053975?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+European+journal+of+neuroscience&rft.atitle=Selective+dopamine+D3+receptor+antagonism+by+SB-277011A+attenuates+cocaine+reinforcement+as+assessed+by+progressive-ratio+and+variable-cost-variable-payoff+fixed-ratio+cocaine+self-administration+in+rats.&rft.au=Xi%2C+Zheng-Xiong%3BGilbert%2C+Jeremy+G%3BPak%2C+Arlene+C%3BAshby%2C+Charles+R%3BHeidbreder%2C+Christian+A%3BGardner%2C+Eliot+L&rft.aulast=Xi&rft.aufirst=Zheng-Xiong&rft.date=2005-06-01&rft.volume=21&rft.issue=12&rft.spage=3427&rft.isbn=&rft.btitle=&rft.title=The+European+journal+of+neuroscience&rft.issn=0953816X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-14 N1 - Date created - 2005-07-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Brain Res. 1992 Jun 12;582(2):349-52 [1356585] Psychopharmacology (Berl). 1991;105(2):151-6 [1839063] Science. 1993 Jun 18;260(5115):1814-6 [8099761] Brain Res. 1993 Oct 8;624(1-2):245-52 [8252397] Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11271-5 [7972046] Drug Alcohol Depend. 1994 Aug;36(1):23-5 [7988355] Psychopharmacology (Berl). 1993;111(2):215-8 [7870955] Psychopharmacology (Berl). 1993;112(2-3):163-82 [7871016] Curr Opin Neurobiol. 1996 Apr;6(2):243-51 [8725967] J Neurosci Methods. 1996 May;66(1):1-11 [8794935] Psychopharmacology (Berl). 1996 Feb;123(3):280-8 [8833421] Annu Rev Neurosci. 1996;19:319-40 [8833446] Pharmacol Biochem Behav. 1997 Jul;57(3):441-7 [9218268] Neuroreport. 1997 Jul 7;8(9-10):2373-7 [9243643] Pharmacol Rev. 1997 Sep;49(3):231-52 [9311022] J Neurosci. 1997 Nov 1;17(21):8580-7 [9334429] Synapse. 1998 Oct;30(2):181-93 [9723788] Psychopharmacology (Berl). 1998 Oct;139(3):169-84 [9784071] Prog Neurobiol. 1998 Dec;56(6):613-72 [9871940] Eur J Pharmacol. 1999 Jun 30;375(1-3):13-30 [10443561] Psychopharmacologia. 1960 Feb 12;1:251-6 [13834123] Recent Adv Biol Psychiatry. 1961;4:288-309 [13916635] Psychopharmacology (Berl). 2005 May;179(3):567-75 [15619116] Pharmacol Biochem Behav. 2005 May;81(1):190-7 [15894078] Brain Res Brain Res Rev. 2005 Jul;49(1):77-105 [15960988] J Med Chem. 2000 May 4;43(9):1878-85 [10794704] J Pharmacol Exp Ther. 2000 Sep;294(3):1154-65 [10945872] J Pharmacol Exp Ther. 2000 Dec;295(3):1223-31 [11082459] Am J Addict. 2000 Fall;9(4):285-313 [11155784] Psychopharmacology (Berl). 2000 Dec;153(1):1-16 [11255919] Xenobiotica. 2001 Aug-Sep;31(8-9):677-86 [11569533] Brain Res Brain Res Rev. 2001 Oct;36(2-3):129-38 [11690609] J Subst Abuse Treat. 2001 Oct;21(3):111-7 [11728784] Pharmacol Rev. 2002 Mar;54(1):1-42 [11870259] J Neurosci. 2002 Nov 1;22(21):9595-603 [12417684] Neuroreport. 2003 Jan 20;14(1):93-8 [12544838] Neurobiol Learn Mem. 2002 Nov;78(3):625-36 [12559840] Neuropsychopharmacology. 2003 Feb;28(2):329-38 [12589386] Synapse. 2003 Jun 1;48(3):154-6 [12645041] Neuropsychopharmacology. 2003 May;28(5):839-49 [12637956] Neuropsychopharmacology. 2003 Jul;28(7):1272-80 [12700694] Brain Res Bull. 2003 Oct 15;61(6):595-601 [14519456] Neurosci Biobehav Rev. 2004 Jan;27(8):803-12 [15019429] Behav Brain Res. 2004 May 5;151(1-2):83-91 [15084424] Psychopharmacology (Berl). 2004 Oct;176(1):57-65 [15083257] Science. 1975 Feb 14;187(4176):547-9 [1114313] Pharmacol Rev. 1975 Sep;27(3):419-28 [817312] Psychopharmacology (Berl). 1976 Aug 17;48(3):311-8 [823588] Life Sci. 1977 Feb 1;20(3):483-92 [839974] Pharmacol Biochem Behav. 1977 Jan;6(1):61-71 [403529] Can J Psychol. 1977 Dec;31(4):195-203 [608135] Psychopharmacology (Berl). 1978 Jul 19;58(3):289-96 [98800] Pharmacol Biochem Behav. 1980 May;12(5):781-7 [7393973] Psychopharmacology (Berl). 1982;78(3):204-9 [6296898] J Pharmacol Exp Ther. 1984 Sep;230(3):678-83 [6332190] Behav Brain Res. 1987 Oct;26(1):57-62 [3675835] Brain Res. 1987 Dec 8;436(1):169-72 [2961413] Psychopharmacology (Berl). 1987;93(4):526-8 [3124187] Eur J Pharmacol. 1988 Jul 7;151(2):233-42 [2844553] Annu Rev Psychol. 1989;40:191-225 [2648975] Psychopharmacology (Berl). 1989;97(4):535-8 [2498950] Pharmacol Biochem Behav. 1989 Jan;32(1):43-7 [2499893] Neurosci Biobehav Rev. 1989 Summer-Fall;13(2-3):123-8 [2530477] Behav Neurosci. 1993 Feb;107(1):161-5 [8095392] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functionally defective or altered CYP3A4 and CYP3A5 single nucleotide polymorphisms and their detection with genotyping tests. AN - 68021692; 16004554 AB - Among the four cytochrome P450 (CYP)3A genes, CYP3A4 and CYP3A5 are the most abundantly expressed in the human liver. Eighty single nucleotide polymorphisms (SNPs) of CYP3A4/5 have been reported to the Human P450 Allele Nomenclature Committee. CYP3A4 alleles with minimal function compared with wild type include the CYP3A4*6 and CYP3A4*17. Alleles with moderately decreased or altered activity include: CYP3A4*2, *8, *11, *12, *13, *16, and *18. CYP3A5 alleles with minimal function include the splice variants CYP3A5*3, *5, *6 and CYP3A5* 10, as well as the null allele CYP3A5*7. Alleles with moderately decreased catalytic activity include CYP3A5*8 and CYP3A5*9. This report reviews the current progress in the functional characterization of CYP3A4 and CYP3A5 SNPs and provides genotyping tests for possible defective variants. A combination of genotyping tests for defective CYP3A4/CYP3A5 haplotypes will be necessary to understand the variations in the metabolism and clinical toxicity of a wide variety of clinical drugs, since these two CYP proteins have overlapping substrate specificities. JF - Pharmacogenomics AU - Lee, Su-Jun AU - Goldstein, Joyce A AD - National Institutes of Health, National Institute of Environmental Health Science Center, NC 27709,USA. lee13@niehs.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 357 EP - 371 VL - 6 IS - 4 SN - 1462-2416, 1462-2416 KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - CYP3A4 protein, human KW - EC 1.14.13.67 KW - CYP3A5 protein, human KW - EC 1.14.14.1 KW - Cytochrome P-450 CYP3A KW - Index Medicus KW - Genotype KW - Polymorphism, Single Nucleotide KW - Humans KW - Cytochrome P-450 Enzyme System -- genetics KW - Cytochrome P-450 Enzyme System -- metabolism KW - Pharmacogenetics -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68021692?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacogenomics&rft.atitle=Functionally+defective+or+altered+CYP3A4+and+CYP3A5+single+nucleotide+polymorphisms+and+their+detection+with+genotyping+tests.&rft.au=Lee%2C+Su-Jun%3BGoldstein%2C+Joyce+A&rft.aulast=Lee&rft.aufirst=Su-Jun&rft.date=2005-06-01&rft.volume=6&rft.issue=4&rft.spage=357&rft.isbn=&rft.btitle=&rft.title=Pharmacogenomics&rft.issn=14622416&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-06-14 N1 - Date created - 2005-07-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Allosteric modulation of the adenosine family of receptors. AN - 67967124; 15974932 AB - Allosteric modulators for adenosine receptors (ARs) are of an increasing interest and may have potential therapeutic advantage over orthosteric ligands. Benzoylthiophene derivatives (including PD 81,723), 2-aminothiazolium salts, and related allosteric modulators of the A(1) AR have been studied. The benzoylthiophene derivatives were demonstrated to be selective enhancers for the A(1) AR, with little or no effect on other subtypes of ARs. Allosteric modulation of the A(2A) AR has also been reported. A(3) allosteric enhancers may be predicted to be useful against ischemic conditions. We have recently characterized two classes of A(3) AR allosteric modulators: 3-(2-pyridinyl)isoquinolines (e.g. VUF5455) and 1H-imidazo-[4,5-c]quinolin-4-amines (e.g. DU124183), which selectively decreased the agonist dissociation rate at the human A(3)AR but not at A(1) and A(2A) ARs. DU124183 left-shifted the agonist conc.-response curve for inhibition of forskolin-stimulated cAMP accumulation in intact cells expressing the human A(3)AR with up to 30% potentiation of the maximal efficacy. The increased potency of A(3) agonists was evident only in the presence of an A(3) antagonist, since VUF5455 and DU124183 also antagonized, i.e. displaced binding at the orthosteric site, with K(i) values of 1.68 and 0.82 microM, respectively. A(3)AR mutagenesis studies implicated F182(5.43) and N274(7.45) in the action of the enhancers and was interpreted using a rhodopsin-based A(3)AR molecular model, suggesting multiple binding modes. Amiloride analogues, SCH-202676 (N-(2,3-diphenyl-1,2,4-thiadiazol-5(2H)-ylidene)methanamine), and sodium ions were demonstrated to be common allosteric modulators for at least three subtypes (A(1), A(2A), and A(3)) of ARs. JF - Mini reviews in medicinal chemistry AU - Gao, Zhan-Guo AU - Kim, Soo-Kyung AU - Ijzerman, Adriaan P AU - Jacobson, Kenneth A AD - Molecular Recognition Section, Laboratory of Bioorganic Chemistry, NIDDK, National Inst. of Health, Bethesda, Maryland 20892-0810, USA. kajobs@helix.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 545 EP - 553 VL - 5 IS - 6 SN - 1389-5575, 1389-5575 KW - Receptor, Adenosine A1 KW - 0 KW - Receptor, Adenosine A2A KW - Receptor, Adenosine A2B KW - Receptors, Purinergic P1 KW - Index Medicus KW - Animals KW - Receptor, Adenosine A2A -- chemistry KW - Receptor, Adenosine A1 -- chemistry KW - Receptor, Adenosine A2B -- chemistry KW - Models, Molecular KW - Humans KW - Receptor, Adenosine A2B -- drug effects KW - Receptor, Adenosine A2A -- drug effects KW - Receptor, Adenosine A1 -- drug effects KW - Mutagenesis KW - Receptors, Purinergic P1 -- chemistry KW - Receptors, Purinergic P1 -- genetics KW - Receptors, Purinergic P1 -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67967124?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mini+reviews+in+medicinal+chemistry&rft.atitle=Allosteric+modulation+of+the+adenosine+family+of+receptors.&rft.au=Gao%2C+Zhan-Guo%3BKim%2C+Soo-Kyung%3BIjzerman%2C+Adriaan+P%3BJacobson%2C+Kenneth+A&rft.aulast=Gao&rft.aufirst=Zhan-Guo&rft.date=2005-06-01&rft.volume=5&rft.issue=6&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Mini+reviews+in+medicinal+chemistry&rft.issn=13895575&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-06-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Bioorg Med Chem Lett. 2002 Feb 11;12(3):501-3 [11814828] Pain. 2002 May;97(1-2):117-25 [12031785] Pharmacol Rev. 2002 Jun;54(2):323-74 [12037145] Bioorg Med Chem Lett. 2002 Jun 17;12(12):1563-6 [12039562] Mol Pharmacol. 2002 Jul;62(1):81-9 [12065758] Nat Rev Drug Discov. 2002 Mar;1(3):198-210 [12120504] Cochrane Database Syst Rev. 2002;(3):CD001747 [12137632] Biochem Pharmacol. 2003 Feb 15;65(4):525-34 [12566079] J Med Chem. 2003 Feb 27;46(5):794-809 [12593659] Mol Pharmacol. 2003 May;63(5):1021-31 [12695530] Life Sci. 2004 May 7;74(25):3173-80 [15081581] Mol Pharmacol. 1987 Jul;32(1):53-8 [3037303] Mol Pharmacol. 1990 Dec;38(6):939-49 [2174510] Mol Pharmacol. 2001 Nov;60(5):1057-63 [11641434] Pharmacol Rev. 2001 Dec;53(4):527-52 [11734617] J Med Chem. 2002 Jan 17;45(2):382-9 [11784142] Anesthesiology. 2001 Aug;95(2):416-20 [11506115] Biochem Pharmacol. 2001 Jan 15;61(2):137-44 [11163328] Mol Pharmacol. 2001 Jan;59(1):30-7 [11125021] Circ Res. 1994 Dec;75(6):972-80 [7955151] Mol Pharmacol. 1996 Jul;50(1):104-11 [8700102] Circ Res. 1996 Sep;79(3):415-23 [8781475] Eur J Pharmacol. 1996 Aug 29;310(2-3):269-72 [8884226] Circulation. 1996 Nov 15;94(10):2551-9 [8921800] Mol Pharmacol. 1996 Dec;50(6):1635-42 [8967987] J Pharmacol Exp Ther. 1997 May;281(2):761-8 [9152383] Br J Pharmacol. 1997 Jul;121(6):1217-23 [9249260] J Med Chem. 1997 Aug 1;40(16):2588-95 [9258366] Mol Pharmacol. 1998 May;53(5):916-25 [9584219] J Pharmacol Exp Ther. 1999 Feb;288(2):446-54 [9918544] J Med Chem. 1999 Sep 9;42(18):3629-35 [10479294] Brain Res. 1999 Sep 4;840(1-2):75-83 [10517954] Circ Res. 1994 Dec;75(6):961-71 [7955150] Am J Physiol. 1993 Mar;264(3 Pt 2):H1017-22 [8456969] Neurochem Int. 1992 Feb;20(2):207-13 [1304860] Eur J Pharmacol. 1993 Jan 4;244(1):21-7 [8420789] J Biol Chem. 1990 Dec 15;265(35):21590-5 [2174879] Mol Pharmacol. 1990 Dec;38(6):950-8 [2250667] Br J Pharmacol. 2000 Jul;130(5):1045-59 [10882389] Biochem Pharmacol. 2000 Sep 1;60(5):661-8 [10927024] Biochem Pharmacol. 2000 Sep 1;60(5):669-76 [10927025] Bioorg Med Chem Lett. 2000 Sep 4;10(17):1953-7 [10987425] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Estrogen-induced changes in IGF-I, Myb family and MAP kinase pathway genes in human uterine leiomyoma and normal uterine smooth muscle cell lines. AN - 67960096; 15879465 AB - Many studies have implicated numerous hormones, growth factors, cytokines and other signal transduction molecules in the pathogenesis of uterine leiomyoma. Estrogen and estrogen-related genes are thought to play a key role in the growth of uterine leiomyomas, but the molecular mechanisms are unclear. In an attempt to investigate various pathways that might be involved in estrogen-regulated uterine leiomyoma growth as well as to identify any novel effector genes, microarray studies comparing estrogen-treated uterine leiomyoma cells (UtLM) and normal myometrial cells to untreated cells were performed. Several genes were differentially expressed in estrogen treated UtLM cells, including insulin-like growth factor-I (IGF-I) and others potentially involved in the IGF-I signalling pathway, specifically genes for A-myb, a transcription factor which promotes cell cycle progression and for MKP-1, a dual specificity phosphatase that dephosphorylates mitogen-activated protein kinase. IGF-I and A-myb were up-regulated in estrogen-treated cells while MKP-1 was down-regulated. Two other cell cycle promoting genes, c-fos and myc, were also down-regulated in estrogen treated UtLM cells. These genes are typically up-regulated in response to estrogen in some cells, notably breast epithelial cells, yet consistently have lower expression levels in uterine leiomyoma tissue when compared to autologous myometrium. Our results demonstrate some novel genes that may play a role in the growth of uterine leiomyoma, strengthen the case for involvement of the IGF-I pathway in the response of UtLM to estrogen and corroborate evidence that uterine smooth muscle cells respond to estrogen with a different gene expression pattern than that seen in epithelial cells. JF - Molecular human reproduction AU - Swartz, C D AU - Afshari, C A AU - Yu, L AU - Hall, K E AU - Dixon, D AD - Laboratory of Experimental Pathology and Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 441 EP - 450 VL - 11 IS - 6 SN - 1360-9947, 1360-9947 KW - Cell Cycle Proteins KW - 0 KW - Estrogens KW - Immediate-Early Proteins KW - MYBL1 protein, human KW - Proto-Oncogene Proteins KW - Trans-Activators KW - Insulin-Like Growth Factor I KW - 67763-96-6 KW - Phosphoprotein Phosphatases KW - EC 3.1.3.16 KW - Protein Phosphatase 1 KW - DUSP1 protein, human KW - EC 3.1.3.48 KW - Dual Specificity Phosphatase 1 KW - Protein Tyrosine Phosphatases KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Protein Tyrosine Phosphatases -- genetics KW - Humans KW - Myocytes, Smooth Muscle -- metabolism KW - Gene Expression Regulation, Neoplastic KW - Myocytes, Smooth Muscle -- drug effects KW - Immediate-Early Proteins -- genetics KW - Uterus -- cytology KW - Tumor Cells, Cultured KW - Cell Cycle Proteins -- genetics KW - Phosphoprotein Phosphatases -- genetics KW - Up-Regulation KW - Female KW - Leiomyoma -- metabolism KW - Trans-Activators -- metabolism KW - Leiomyoma -- genetics KW - Uterine Neoplasms -- genetics KW - Leiomyoma -- immunology KW - Estrogens -- metabolism KW - Proto-Oncogene Proteins -- analysis KW - Proto-Oncogene Proteins -- metabolism KW - Trans-Activators -- analysis KW - Insulin-Like Growth Factor I -- analysis KW - Insulin-Like Growth Factor I -- metabolism KW - MAP Kinase Signaling System -- genetics KW - Insulin-Like Growth Factor I -- genetics KW - Uterine Neoplasms -- immunology KW - Estrogens -- pharmacology KW - Trans-Activators -- genetics KW - Uterine Neoplasms -- metabolism KW - Proto-Oncogene Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67960096?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+human+reproduction&rft.atitle=Estrogen-induced+changes+in+IGF-I%2C+Myb+family+and+MAP+kinase+pathway+genes+in+human+uterine+leiomyoma+and+normal+uterine+smooth+muscle+cell+lines.&rft.au=Swartz%2C+C+D%3BAfshari%2C+C+A%3BYu%2C+L%3BHall%2C+K+E%3BDixon%2C+D&rft.aulast=Swartz&rft.aufirst=C&rft.date=2005-06-01&rft.volume=11&rft.issue=6&rft.spage=441&rft.isbn=&rft.btitle=&rft.title=Molecular+human+reproduction&rft.issn=13609947&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-06-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Endocannabinoid signaling system and brain reward: emphasis on dopamine. AN - 67954176; 15936806 AB - The brain's reward circuitry consists of an "in series" circuit of dopaminergic (DA) neurons in the ventral tegmental area (VTA), nucleus accumbens (Acb), and that portion of the medial forebrain bundle (MFB) which links the VTA and Acb. Drugs which enhance brain reward (and have derivative addictive potential) have common actions on this core DA reward system and on animal behaviors relating to its function. Such drugs enhance electrical brain-stimulation reward in this reward system; enhance neural firing and DA tone within it; produce conditioned place preference (CPP), a behavioral model of incentive motivation; are self-administered; and trigger reinstatement of drug-seeking behavior in animals extinguished from drug self-administration. Cannabinoids were long considered different from other reward-enhancing drugs in reward efficacy and in underlying neurobiological substrates activated. However, it is now clear that cannabinoids activate these brain reward processes and reward-related behaviors in similar fashion to other reward-enhancing drugs. This brief review discusses the roles that endogenous cannabinoids (especially activation of the CB1 receptor) may play within the core reward system, and concludes that while cannabinoids activate the reward pathways in a manner consistent with other reward-enhancing drugs, the neural mechanisms by which this occurs may differ. JF - Pharmacology, biochemistry, and behavior AU - Gardner, Eliot L AD - Neuropsychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Building C-Room 393, Baltimore, MD 21224, USA. egardner@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 263 EP - 284 VL - 81 IS - 2 SN - 0091-3057, 0091-3057 KW - Cannabinoid Receptor Modulators KW - 0 KW - Cannabinoids KW - Endocannabinoids KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Substance-Related Disorders -- physiopathology KW - Behavior -- drug effects KW - Animals KW - Self Administration KW - Humans KW - Brain Chemistry -- drug effects KW - Substance-Related Disorders -- psychology KW - Cannabinoids -- pharmacology KW - Brain Chemistry -- physiology KW - Signal Transduction -- physiology KW - Cannabinoid Receptor Modulators -- physiology KW - Reward KW - Brain -- anatomy & histology KW - Dopamine -- physiology KW - Dopamine -- metabolism KW - Brain -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67954176?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacology%2C+biochemistry%2C+and+behavior&rft.atitle=Endocannabinoid+signaling+system+and+brain+reward%3A+emphasis+on+dopamine.&rft.au=Gardner%2C+Eliot+L&rft.aulast=Gardner&rft.aufirst=Eliot&rft.date=2005-06-01&rft.volume=81&rft.issue=2&rft.spage=263&rft.isbn=&rft.btitle=&rft.title=Pharmacology%2C+biochemistry%2C+and+behavior&rft.issn=00913057&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-08 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevalence, correlates, and comorbidity of DSM-IV antisocial personality syndromes and alcohol and specific drug use disorders in the United States: results from the national epidemiologic survey on alcohol and related conditions. AN - 67949601; 15960559 AB - The purpose of this study was to provide nationally representative data on the prevalence, sociodemographic correlates, and comorbidity of antisocial syndromes across alcohol and 8 specific drug use disorders, including sedative, tranquilizer, opiate, stimulant, hallucinogen, cannabis, cocaine, and inhalant/solvent abuse and dependence. This study is based on a nationally representative sample of adults. Lifetime prevalences of antisocial syndromes were estimated and logistic regression analyses were used to examine associations between antisocial syndromes and sociodemographic characteristics and substance use disorders. Diagnoses were made according to the criteria of the DSM-IV using the National Institute on Alcohol Abuse and Alcoholism Alcohol Use Disorder and Associated Disabilities Interview Schedule-DSM-IV Version. The lifetime prevalences of antisocial personality disorder (APD), conduct disorder, and adult antisocial behavior were 3.6%, 1.1%, and 12.3%, respectively. Prevalences of alcohol use disorders and drug use disorders were 30.3% and 10.3%, respectively. In general, men and individuals who were younger, widowed/separated/divorced, of lower socioeconomic status, and living in urban areas or in the West were more likely to have antisocial syndromes. Native Americans were more likely and Asians and Hispanics were less likely to have APD and adult antisocial behavior. Virtually all of the associations between APD and adult antisocial behavior and specific substance use disorders were positive and statistically significant (p < .05). Significant associations between conduct disorder and substance use disorders were concentrated among women. Comorbidity of specific substance disorders with antisocial syndromes is very common in the U.S. population. Further work in many directions is indicated by the results of this study, including the factors that give rise to the associations and the treatment and prevention implications of these conditions when comorbid. JF - The Journal of clinical psychiatry AU - Compton, Wilson M AU - Conway, Kevin P AU - Stinson, Frederick S AU - Colliver, James D AU - Grant, Bridget F AD - Division of Epidemiology, Services, and Prevention Research, National Institute on Drug Abuse National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 677 EP - 685 VL - 66 IS - 6 SN - 0160-6689, 0160-6689 KW - Index Medicus KW - Humans KW - Aged KW - Comorbidity KW - Epidemiologic Studies KW - Adult KW - Health Surveys KW - Middle Aged KW - Psychiatric Status Rating Scales -- statistics & numerical data KW - Adolescent KW - United States -- epidemiology KW - Diagnostic and Statistical Manual of Mental Disorders KW - Female KW - Male KW - Prevalence KW - Substance-Related Disorders -- diagnosis KW - Antisocial Personality Disorder -- epidemiology KW - Alcoholism -- epidemiology KW - Alcoholism -- diagnosis KW - Antisocial Personality Disorder -- diagnosis KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67949601?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+clinical+psychiatry&rft.atitle=Prevalence%2C+correlates%2C+and+comorbidity+of+DSM-IV+antisocial+personality+syndromes+and+alcohol+and+specific+drug+use+disorders+in+the+United+States%3A+results+from+the+national+epidemiologic+survey+on+alcohol+and+related+conditions.&rft.au=Compton%2C+Wilson+M%3BConway%2C+Kevin+P%3BStinson%2C+Frederick+S%3BColliver%2C+James+D%3BGrant%2C+Bridget+F&rft.aulast=Compton&rft.aufirst=Wilson&rft.date=2005-06-01&rft.volume=66&rft.issue=6&rft.spage=677&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+clinical+psychiatry&rft.issn=01606689&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-04 N1 - Date created - 2005-06-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Critical window of male reproductive tract development in rats following gestational exposure to di-n-butyl phthalate. AN - 67944775; 15954088 AB - Gestational exposure to di-n-butyl phthalate (DBP), a ubiquitous environmental contaminant, has been shown to interfere with the development of the male reproductive tract by acting as an antiandrogen. This study was conducted to identify the critical days for the abnormal development of the male reproductive tract, specifically the testis and epididymis. Timed-pregnant Sprague-Dawley rats were dosed with DBP at 500 mg/kg/day on gestation day (GD) 14 and 15, 15 and 16, 16 and 17, 17 and 18, 18 and 19, or 19 and 20 (GD 0=plug day). Anogenital distance (AGD) was measured on postnatal day (PND) 1 and 13, while areloa number was recorded on PND 13 only. After weaning, males were allowed to mature to PND 90 at which time they were necropsied. Areloa number and AGD were recorded and testes, epididymides, seminal vesicles, prostate gland, kidneys, and liver weighed. Blood serum was collected and assayed for total testosterone concentration. There were no observable effects on litter size, sex ratio, serum testosterone concentration, or mortality of pups. Statistically significant permanent reductions in AGD were seen in males exposed prenatally to DBP on GD 15 and 16 or GD 18 and 19. On PND 13, areola were present in males exposed to DBP on GD 15 and 16, 16 and 17, 17 and 18, and 19 and 20. However, permanent retention occurred only in males after DBP exposure on GD 16 and 17. Exposure to DBP on only GD 17 and 18 elicited a reduction in epididymal weights; while exposure on only GD 16 and 17 caused a significant increase in the weights of the testes due to edema. In this study, epididymal and testicular malformations were most prevalent after exposure to DBP on any gestational day. Epididymal malformations, characterized by agenesis of various regions and small or flaccid testes were significantly increased in DBP-exposed males only on GD 16 and 17. These findings suggest that 2-day DBP exposure is highly detrimental to the developing reproductive tract of the male fetus and the critical window for abnormal development is GD 16-18. JF - Birth defects research. Part B, Developmental and reproductive toxicology AU - Carruthers, Christina M AU - Foster, Paul M D AD - National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA. carruth1@niehs.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 277 EP - 285 VL - 74 IS - 3 SN - 1542-9733, 1542-9733 KW - Dibutyl Phthalate KW - 2286E5R2KE KW - Testosterone KW - 3XMK78S47O KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Nipples -- abnormalities KW - Testosterone -- blood KW - Male KW - Female KW - Pregnancy KW - Organ Size -- drug effects KW - Abnormalities, Drug-Induced KW - Genitalia, Male -- cytology KW - Genitalia, Male -- abnormalities KW - Genitalia, Male -- growth & development KW - Dibutyl Phthalate -- toxicity KW - Maternal Exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67944775?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Birth+defects+research.+Part+B%2C+Developmental+and+reproductive+toxicology&rft.atitle=Critical+window+of+male+reproductive+tract+development+in+rats+following+gestational+exposure+to+di-n-butyl+phthalate.&rft.au=Carruthers%2C+Christina+M%3BFoster%2C+Paul+M+D&rft.aulast=Carruthers&rft.aufirst=Christina&rft.date=2005-06-01&rft.volume=74&rft.issue=3&rft.spage=277&rft.isbn=&rft.btitle=&rft.title=Birth+defects+research.+Part+B%2C+Developmental+and+reproductive+toxicology&rft.issn=15429733&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-07 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Primary thyroid cancer after a first tumour in childhood (the Childhood Cancer Survivor Study): a nested case-control study. AN - 67924132; 15950715 AB - Survivors of malignant disease in childhood who have had radiotherapy to the head, neck, or upper thorax have an increased risk of subsequent primary thyroid cancer, but the magnitude of risk over the therapeutic dose range has not been well established. We aimed to quantify the long-term risk of thyroid cancer after radiotherapy and chemotherapy. In a nested case-control study, 69 cases with pathologically confirmed thyroid cancer and 265 matched controls without thyroid cancer were identified from 14,054 5-year survivors of cancer during childhood from the Childhood Cancer Survivor Study cohort. Childhood cancers were diagnosed between 1970 and 1986 with cohort follow-up to 2000. Risk of thyroid cancer increased with radiation doses up to 20-29 Gy (odds ratio 9.8 [95% CI 3.2-34.8]). At doses greater than 30 Gy, a fall in the dose-response relation was seen. Both the increased and decreased risks were more pronounced in those diagnosed with a first primary malignant disease before age 10 years than in those older than 10 years. Furthermore, the fall in risk remained when those diagnosed with Hodgkin's lymphoma were excluded. Chemotherapy for the first cancer was not associated with thyroid-cancer risk, and it did not modify the effect of radiotherapy. 29 (42%) cases had a first diagnosis of Hodgkin's lymphoma compared with 49 (19%) controls. 11 (42%) of those who had Hodgkin's lymphoma had subsequent thyroid cancers smaller than 1 cm compared with six (17%) of those who had other types of childhood cancer (p=0.07). The reduction in radiation dose-response for risk of thyroid cancer after childhood exposure to thyroid doses higher than 30 Gy is consistent with a cell-killing effect. Standard long-term follow-up of patients who have had Hodgkin's lymphoma for detection of thyroid cancer should also be undertaken for survivors of any cancer during childhood who received radiotherapy to the thorax or head and neck region. JF - Lancet (London, England) AU - Sigurdson, Alice J AU - Ronckers, Cécile M AU - Mertens, Ann C AU - Stovall, Marilyn AU - Smith, Susan A AU - Liu, Yan AU - Berkow, Roger L AU - Hammond, Sue AU - Neglia, Joseph P AU - Meadows, Anna T AU - Sklar, Charles A AU - Robison, Leslie L AU - Inskip, Peter D AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20892-7238, USA. sigurdsa@mail.nih.gov PY - 2005 SP - 2014 EP - 2023 VL - 365 IS - 9476 KW - Abridged Index Medicus KW - Index Medicus KW - Neoplasms, Second Primary -- etiology KW - Radiotherapy Dosage KW - Humans KW - Adult KW - Case-Control Studies KW - Child KW - Dose-Response Relationship, Radiation KW - Adolescent KW - Male KW - Female KW - Neoplasms, Radiation-Induced KW - Thyroid Neoplasms -- etiology KW - Thyroid Gland -- radiation effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67924132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lancet+%28London%2C+England%29&rft.atitle=Primary+thyroid+cancer+after+a+first+tumour+in+childhood+%28the+Childhood+Cancer+Survivor+Study%29%3A+a+nested+case-control+study.&rft.au=Sigurdson%2C+Alice+J%3BRonckers%2C+C%C3%A9cile+M%3BMertens%2C+Ann+C%3BStovall%2C+Marilyn%3BSmith%2C+Susan+A%3BLiu%2C+Yan%3BBerkow%2C+Roger+L%3BHammond%2C+Sue%3BNeglia%2C+Joseph+P%3BMeadows%2C+Anna+T%3BSklar%2C+Charles+A%3BRobison%2C+Leslie+L%3BInskip%2C+Peter+D&rft.aulast=Sigurdson&rft.aufirst=Alice&rft.date=2005-06-01&rft.volume=365&rft.issue=9476&rft.spage=2014&rft.isbn=&rft.btitle=&rft.title=Lancet+%28London%2C+England%29&rft.issn=1474-547X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-06 N1 - Date created - 2005-06-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Lancet. 2005 Jun 11-17;365(9476):1986-7 [15950701] Lancet. 2005 Sep 3-9;366(9488):805 [16139649] Lancet. 2005 Sep 3-9;366(9488):804-5 [16139647] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Small molecule inhibitors of HDM2 ubiquitin ligase activity stabilize and activate p53 in cells. AN - 67920711; 15950904 AB - The p53 tumor suppressor protein is regulated by its interaction with HDM2, which serves as a ubiquitin ligase (E3) to target p53 for degradation. We have identified a family of small molecules (HLI98) that inhibits HDM2's E3 activity. These compounds show some specificity for HDM2 in vitro, although at higher concentrations effects on unrelated RING and HECT domain E3s are detectable, which could be due, at least in part, to effects on E2-ubiquitin thiol-ester levels. In cells, the compounds allow the stabilization of p53 and HDM2 and activation of p53-dependent transcription and apoptosis, although other p53-independent toxicity was also observed. JF - Cancer cell AU - Yang, Yili AU - Ludwig, Robert L AU - Jensen, Jane P AU - Pierre, Shervon A AU - Medaglia, Maxine V AU - Davydov, Ilia V AU - Safiran, Yassamin J AU - Oberoi, Pankaj AU - Kenten, John H AU - Phillips, Andrew C AU - Weissman, Allan M AU - Vousden, Karen H AD - Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute at Frederick, NIH, 1050 Boyles Street, Frederick, MD 21702, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 547 EP - 559 VL - 7 IS - 6 SN - 1535-6108, 1535-6108 KW - CDKN1A protein, human KW - 0 KW - Cdkn1a protein, mouse KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - Endosomal Sorting Complexes Required for Transport KW - Enzyme Inhibitors KW - Flavins KW - Nuclear Proteins KW - Proteins KW - Proto-Oncogene Proteins KW - Tumor Suppressor Protein p53 KW - Ubiquitin KW - 5-deazaflavin KW - 26908-38-3 KW - UBE3A protein, human KW - EC 2.3.2.26 KW - MDM2 protein, human KW - EC 2.3.2.27 KW - Mdm2 protein, mouse KW - Proto-Oncogene Proteins c-mdm2 KW - Ubiquitin-Protein Ligases KW - seven in absentia proteins KW - Poly(ADP-ribose) Polymerases KW - EC 2.4.2.30 KW - CDK4 protein, human KW - EC 2.7.11.22 KW - Cdk4 protein, mouse KW - Cyclin-Dependent Kinase 4 KW - Cyclin-Dependent Kinases KW - Caspases KW - EC 3.4.22.- KW - Nedd4 ubiquitin protein ligases KW - EC 6.3.2.- KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Humans KW - Poly(ADP-ribose) Polymerases -- metabolism KW - Caspases -- metabolism KW - Epithelial Cells -- metabolism KW - Apoptosis -- drug effects KW - Flavins -- chemistry KW - Ubiquitin-Protein Ligases -- antagonists & inhibitors KW - Cyclin-Dependent Kinases -- metabolism KW - Molecular Structure KW - Fibroblasts -- drug effects KW - Cell Line, Tumor KW - Mice KW - Fibroblasts -- metabolism KW - Proteins -- metabolism KW - Cell Cycle Proteins -- metabolism KW - Phosphorylation -- drug effects KW - Ubiquitin -- metabolism KW - Epithelial Cells -- drug effects KW - Proteins -- antagonists & inhibitors KW - Transfection KW - Protein Binding -- drug effects KW - Ubiquitin-Protein Ligases -- metabolism KW - Cell Line KW - Proto-Oncogene Proteins -- antagonists & inhibitors KW - Nuclear Proteins -- genetics KW - Enzyme Inhibitors -- chemistry KW - Proto-Oncogene Proteins -- metabolism KW - Enzyme Inhibitors -- pharmacology KW - Tumor Suppressor Protein p53 -- genetics KW - Proto-Oncogene Proteins -- genetics KW - Nuclear Proteins -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism KW - Nuclear Proteins -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67920711?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+cell&rft.atitle=Small+molecule+inhibitors+of+HDM2+ubiquitin+ligase+activity+stabilize+and+activate+p53+in+cells.&rft.au=Yang%2C+Yili%3BLudwig%2C+Robert+L%3BJensen%2C+Jane+P%3BPierre%2C+Shervon+A%3BMedaglia%2C+Maxine+V%3BDavydov%2C+Ilia+V%3BSafiran%2C+Yassamin+J%3BOberoi%2C+Pankaj%3BKenten%2C+John+H%3BPhillips%2C+Andrew+C%3BWeissman%2C+Allan+M%3BVousden%2C+Karen+H&rft.aulast=Yang&rft.aufirst=Yili&rft.date=2005-06-01&rft.volume=7&rft.issue=6&rft.spage=547&rft.isbn=&rft.btitle=&rft.title=Cancer+cell&rft.issn=15356108&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-06 N1 - Date created - 2005-06-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Anticonvulsant activity of androsterone and etiocholanolone. AN - 67918275; 15946323 AB - Men with epilepsy often have sexual or reproductive abnormalities that are attributed to alterations in androgen levels, including subnormal free testosterone. Levels of the major metabolites of testosterone-androsterone (5alpha-androstan-3alpha-ol-17-one; 5alpha,3alpha-A), a neurosteroid that acts as a positive allosteric modulator of GABA(A) receptors, and its 5beta-epimer etiocholanolone (5beta-androstan-3alpha-ol-17-one; 5beta,3alpha-A)-also may be reduced in epilepsy. 5alpha,3alpha-A has been found in adult brain, and both metabolites, which also can be derived from androstenedione, are present in substantial quantities in serum along with their glucuronide and sulfate conjugates. This study sought to determine whether these endogenous steroid metabolites can protect against seizures. The anticonvulsant activity of 5alpha,3alpha-A and 5beta,3alpha-A was investigated in electrical and chemoconvulsant seizure models in mice. The steroids also were examined for activity against extracellularly recorded epileptiform discharges in the CA3 region of the rat hippocampal slice induced by perfusion with 55 microM 4-aminopyridine (4-AP). Intraperitoneal injection of 5alpha,3alpha-A-protected mice in a dose-dependent fashion from seizures in the following models (ED50, dose in mg/kg protecting 50% of animals): 6-Hz electrical stimulation (29.1), pentylenetetrazol (43.5), pilocarpine (105), 4-AP (215), and maximal electroshock (224). 5beta,3alpha-A also was active in the 6-Hz and pentylenetetrazol models, but was less potent (ED50 values, 76.9 and 139 mg/kg, respectively), whereas epiandrosterone (5alpha,3beta-A) was inactive (ED50, 20 cigarettes/day) (OR = 21.1, 95% CI = 3.9-114.4, P for interaction = 0.03) compared with non-smokers who did not carry either of these variants. These genotypes were unassociated with risk in non-smokers. In analysis of adenoma subtypes, the combined gene variants were most strongly associated with the presence of multiple adenoma (P = 0.002). In summary, joint carriage of CYP1A1 Val(462) and NQO1 Ser(187) alleles, particularly in smokers, was related to colorectal adenoma risk, with a propensity for formation of multiple lesions. JF - Carcinogenesis AU - Hou, Lifang AU - Chatterjee, Nilanjan AU - Huang, Wen-Yi AU - Baccarelli, Andrea AU - Yadavalli, Sunita AU - Yeager, Meredith AU - Bresalier, Robert S AU - Chanock, Stephen J AU - Caporaso, Neil E AU - Ji, Bu-Tian AU - Weissfeld, Joel L AU - Hayes, Richard B AD - Department of Human and Health Services, Division of Cancer Epidemiology and Genetics, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-7240, USA. lifangh2@mail.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1122 EP - 1128 VL - 26 IS - 6 SN - 0143-3334, 0143-3334 KW - Cytochrome P-450 CYP1A1 KW - EC 1.14.14.1 KW - NAD(P)H Dehydrogenase (Quinone) KW - EC 1.6.5.2 KW - NQO1 protein, human KW - Index Medicus KW - Risk KW - Alleles KW - Humans KW - Aged KW - Middle Aged KW - Genetic Predisposition to Disease KW - Male KW - Female KW - NAD(P)H Dehydrogenase (Quinone) -- genetics KW - Cytochrome P-450 CYP1A1 -- genetics KW - Polymorphism, Genetic KW - Colorectal Neoplasms -- pathology KW - Smoking -- adverse effects KW - Colorectal Neoplasms -- genetics KW - Adenoma -- pathology KW - Adenoma -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67888584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=CYP1A1+Val462+and+NQO1+Ser187+polymorphisms%2C+cigarette+use%2C+and+risk+for+colorectal+adenoma.&rft.au=Hou%2C+Lifang%3BChatterjee%2C+Nilanjan%3BHuang%2C+Wen-Yi%3BBaccarelli%2C+Andrea%3BYadavalli%2C+Sunita%3BYeager%2C+Meredith%3BBresalier%2C+Robert+S%3BChanock%2C+Stephen+J%3BCaporaso%2C+Neil+E%3BJi%2C+Bu-Tian%3BWeissfeld%2C+Joel+L%3BHayes%2C+Richard+B&rft.aulast=Hou&rft.aufirst=Lifang&rft.date=2005-06-01&rft.volume=26&rft.issue=6&rft.spage=1122&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-26 N1 - Date created - 2005-06-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitrosative stress and pharmacological modulation of heart failure. AN - 67886031; 15925705 AB - Dysregulation of nitric oxide (NO) and increased oxidative and nitrosative stress are implicated in the pathogenesis of heart failure. Peroxynitrite is a reactive oxidant that is produced from the reaction of nitric oxide with superoxide anion and impairs cardiovascular function through multiple mechanisms, including activation of matrix metalloproteinases (MMPs) and nuclear enzyme poly(ADP-ribose) polymerase (PARP). Recent studies suggest that the neutralization of peroxynitrite or pharmacological inhibition of MMPs and PARP are promising new approaches in the experimental therapy of various forms of myocardial injury. In this article, the role of nitrosative stress and downstream mechanisms, including activation of MMPs and PARP, in various forms of heart failure are discussed and novel emerging therapeutic strategies offered by neutralization of peroxynitrite and inhibition of MMPs and PARP in these pathophysiological conditions are reviewed. JF - Trends in pharmacological sciences AU - Pacher, Pal AU - Schulz, Richard AU - Liaudet, Lucas AU - Szabó, Csaba AD - Laboratory of Physiological Studies, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane MSC 9413, Room 2S24, Bethesda, MD 20892-9413, USA. ppacher@lycos.com Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 302 EP - 310 VL - 26 IS - 6 SN - 0165-6147, 0165-6147 KW - Enzyme Inhibitors KW - 0 KW - Poly(ADP-ribose) Polymerase Inhibitors KW - Peroxynitrous Acid KW - 14691-52-2 KW - Nitric Oxide KW - 31C4KY9ESH KW - Nitric Oxide Synthase KW - EC 1.14.13.39 KW - Poly(ADP-ribose) Polymerases KW - EC 2.4.2.30 KW - Matrix Metalloproteinase 2 KW - EC 3.4.24.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Index Medicus KW - Matrix Metalloproteinase 9 -- metabolism KW - Enzyme Inhibitors -- therapeutic use KW - Enzyme Activation KW - Humans KW - Nitric Oxide Synthase -- physiology KW - Poly(ADP-ribose) Polymerases -- metabolism KW - Peroxynitrous Acid -- toxicity KW - Matrix Metalloproteinase 2 -- metabolism KW - Heart Failure -- drug therapy KW - Oxidative Stress KW - Nitric Oxide -- physiology KW - Heart Failure -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67886031?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Trends+in+pharmacological+sciences&rft.atitle=Nitrosative+stress+and+pharmacological+modulation+of+heart+failure.&rft.au=Pacher%2C+Pal%3BSchulz%2C+Richard%3BLiaudet%2C+Lucas%3BSzab%C3%B3%2C+Csaba&rft.aulast=Pacher&rft.aufirst=Pal&rft.date=2005-06-01&rft.volume=26&rft.issue=6&rft.spage=302&rft.isbn=&rft.btitle=&rft.title=Trends+in+pharmacological+sciences&rft.issn=01656147&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-12 N1 - Date created - 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Last updated - 2017-01-18 ER - TY - JOUR T1 - Conditional transgenic system for mouse aurora a kinase: degradation by the ubiquitin proteasome pathway controls the level of the transgenic protein. AN - 67879482; 15923640 AB - Aurora A is a mitotic kinase that localizes to centrosomes. Expression of this protein is normally limited to the mitotic stage (G(2)-M) of the cell cycle, whereas human cancer cells frequently exhibit overexpression of Aurora A protein regardless of the cell cycle stage. In the present study, Aurora A transgenic mouse lines were generated with a new conditional expression system (cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter-Z-enhanced green fluorescent protein) in order to analyze the function of this protein. Although transcripts for Aurora A were elevated in multiple organs of the transgenic mice, the corresponding protein was not detected in extracts analyzed by immunoblotting. The treatment of transgenic-derived embryonic fibroblasts (MEF) with proteasome inhibitors markedly increased the protein level of transgenic Aurora A, indicating that the transgenic Aurora A protein is readily degraded in normal mouse tissues. Under the exponential growth conditions of MEF cells, transgenic Aurora A was detected within the mitotic stage of the cell cycle and localized to centrosomes. In contrast, the marker of the transgenic promoter (enhanced green fluorescent protein) was continuously expressed throughout the cell cycle, indicating the constitutive transcription of transgenic mRNA. These results indicate that transgenic Aurora A is protected from degradation within G(2)-M but is immediately degraded after translation in the G(1)-S stage of the cell cycle. The findings obtained with this transgenic model and derived cells support that the transition from protection to degradation by the ubiquitin proteasome system at the end of mitosis is an important step in controlling the level of Aurora A protein during the cell cycle. JF - Molecular and cellular biology AU - Fukuda, Tomokazu AU - Mishina, Yuji AU - Walker, Michael P AU - DiAugustine, Richard P AD - Hormones and Cancer Group, Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Mail Drop D4-04, Research Triangle Park, NC 27709, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 5270 EP - 5281 VL - 25 IS - 12 SN - 0270-7306, 0270-7306 KW - Proteasome Inhibitors KW - 0 KW - RNA, Messenger KW - Recombinant Fusion Proteins KW - Ubiquitin KW - Aurka protein, mouse KW - EC 2.7.11.1 KW - Aurora Kinase A KW - Aurora Kinases KW - Protein-Serine-Threonine Kinases KW - Proteasome Endopeptidase Complex KW - EC 3.4.25.1 KW - Index Medicus KW - Animals KW - Embryo, Mammalian -- physiology KW - Kidney -- metabolism KW - Reproducibility of Results KW - Humans KW - Cell Cycle -- physiology KW - Embryo, Mammalian -- anatomy & histology KW - Mice KW - Brain -- metabolism KW - Fibroblasts -- cytology KW - Embryo, Nonmammalian KW - Fibroblasts -- physiology KW - Chickens KW - RNA, Messenger -- metabolism KW - Cells, Cultured KW - Recombination, Genetic KW - Brain -- anatomy & histology KW - Molecular Sequence Data KW - Kidney -- anatomy & histology KW - Recombinant Fusion Proteins -- metabolism KW - Ubiquitin -- metabolism KW - Protein-Serine-Threonine Kinases -- metabolism KW - Proteasome Endopeptidase Complex -- metabolism KW - Recombinant Fusion Proteins -- genetics KW - Protein-Serine-Threonine Kinases -- genetics KW - Mice, Transgenic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67879482?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biology&rft.atitle=Conditional+transgenic+system+for+mouse+aurora+a+kinase%3A+degradation+by+the+ubiquitin+proteasome+pathway+controls+the+level+of+the+transgenic+protein.&rft.au=Fukuda%2C+Tomokazu%3BMishina%2C+Yuji%3BWalker%2C+Michael+P%3BDiAugustine%2C+Richard+P&rft.aulast=Fukuda&rft.aufirst=Tomokazu&rft.date=2005-06-01&rft.volume=25&rft.issue=12&rft.spage=5270&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biology&rft.issn=02707306&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-05-30 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1998 Mar 13;273(11):6373-9 [9497367] Mol Cell Biol. 1994 Jul;14(7):4731-40 [8007975] EMBO J. 1998 Jun 1;17(11):3052-65 [9606188] Int J Cancer. 2001 May 1;92(3):370-3 [11291073] Endocrinology. 2001 Sep;142(9):3842-9 [11517161] Transgenic Res. 2001 Dec;10(6):545-53 [11817542] EMBO J. 2002 Feb 15;21(4):483-92 [11847097] J Biol Chem. 2002 Mar 22;277(12):10719-26 [11790771] Cancer Res. 2002 Jul 15;62(14):4115-22 [12124350] Cell Mol Biol Res. 1994;40(3):207-13 [7874197] Cell. 1995 Apr 7;81(1):95-105 [7720077] J Cell Biol. 1997 Aug 11;138(3):643-56 [9245792] J Cell Sci. 1998 Mar;111 ( Pt 5):557-72 [9454730] Oncogene. 2000 Jun 1;19(24):2812-9 [10851084] Anal Biochem. 2000 Oct 15;285(2):274-6 [11017715] Oncogene. 2000 Oct 5;19(42):4906-16 [11039908] Br J Cancer. 2001 Mar 23;84(6):824-31 [11259099] Genes Dev. 2002 Sep 1;16(17):2274-85 [12208850] Oncogene. 2002 Sep 9;21(40):6175-83 [12214247] Genes Cells. 2002 Nov;7(11):1173-82 [12390251] Nat Rev Cancer. 2002 Nov;2(11):815-25 [12415252] Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15440-5 [12422018] EMBO Rep. 2002 Dec;3(12):1209-14 [12446569] Cancer Cell. 2003 Jan;3(1):51-62 [12559175] Nat Cell Biol. 2003 Mar;5(3):242-8 [12577065] J Cell Biol. 2003 Apr 28;161(2):267-80 [12719470] Nat Genet. 2003 Aug;34(4):403-12 [12881723] J Dent Res. 2003 Oct;82(10):776-80 [14514755] Cancer Epidemiol Biomarkers Prev. 2003 Dec;12(12):1518-22 [14693746] Mol Cell Biol. 2004 Feb;24(4):1809-21 [14749395] J Biol Chem. 2004 Mar 5;279(10):9008-15 [14701852] Nat Genet. 1998 Oct;20(2):189-93 [9771714] Cancer Res. 1999 May 1;59(9):2041-4 [10232583] Trends Cell Biol. 1999 Nov;9(11):454-9 [10511710] Oncogene. 2004 Nov 18;23(54):8720-30 [15480417] J Cell Sci. 2004 Dec 1;117(Pt 25):5975-83 [15536123] Cancer Res. 2004 Apr 15;64(8):2680-3 [15087379] Nucleic Acids Res. 1991 Mar 11;19(5):1154 [2020552] Erratum In: Mol Cell Biol. 2015 Dec 1;35(23):4094 [26518639] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Menstrual cycle length during methadone maintenance. AN - 67874091; 15918813 AB - While the menstrual disruption of heroin has been demonstrated, there are few published data concerning methadone maintenance and menstrual function. This study was conducted to evaluate whether cycle length was more regular during methadone maintenance. An out-patient research treatment program in Baltimore, Maryland, USA. A total of 191 heroin and cocaine-using women from two clinical trials, lasting 25-29 weeks; each woman was maintained on 70-100 mg of methadone. Start/end dates of each menses were collected. Menstrual patterns were classified as regular, irregular, transient amenorrhea, persistent amenorrhea or cycle restart. Repeated-measures regression modeling determined correlates of cycle length and predictors of long cycles (> 40 days) and short cycles (< 20 days). Bleeding episodes were defined as 1 or more bleeding days, bound by at least 2 non-bleeding days. Correlates/predictors examined were body mass index, drug use, methadone dose and race. In the 133 women for whom menstrual patterns could be determined, cycle-length irregularity was common: irregular, 62 (46.7%); regular, 37 (27.8%); cycle restart, 16 (12%); persistent amenorrhea, 11 (8.3%); transient amenorrhea, seven (5.3%). Each additional week on methadone maintenance was associated with decreased risk of long (OR = 0.96, P < 0.01 and short (OR = 0.92, P < 0.01) cycles. Of 27 women with secondary amenorrhea pre-study, 16 (59%) restarted menses. Positivity for opioids or cocaine was not significantly associated with short or long cycles. Cycle length begins to normalize during methadone maintenance. Menses resumption may occur. Methadone maintenance, despite interfering with menstrual function in an absolute sense, may interfere less than illicit heroin abuse. JF - Addiction (Abingdon, England) AU - Schmittner, John AU - Schroeder, Jennifer R AU - Epstein, David H AU - Preston, Kenzie L AD - Intramural Research Program, National Institute on Drug Abuse, National Institute of Health, Baltimore, MD 21224, USA. jschmitt@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 829 EP - 836 VL - 100 IS - 6 SN - 0965-2140, 0965-2140 KW - Narcotics KW - 0 KW - Methadone KW - UC6VBE7V1Z KW - Index Medicus KW - Humans KW - Adult KW - Retrospective Studies KW - Middle Aged KW - Amenorrhea -- chemically induced KW - Female KW - Methadone -- therapeutic use KW - Menstrual Cycle -- physiology KW - Narcotics -- therapeutic use KW - Heroin Dependence -- rehabilitation KW - Menstrual Cycle -- drug effects KW - Menstruation Disturbances -- chemically induced KW - Heroin Dependence -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67874091?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=Menstrual+cycle+length+during+methadone+maintenance.&rft.au=Schmittner%2C+John%3BSchroeder%2C+Jennifer+R%3BEpstein%2C+David+H%3BPreston%2C+Kenzie+L&rft.aulast=Schmittner&rft.aufirst=John&rft.date=2005-06-01&rft.volume=100&rft.issue=6&rft.spage=829&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-05-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Detection and localization of proteinuria by dynamic contrast-enhanced magnetic resonance imaging using MS325. AN - 67868613; 15872075 AB - After renal transplantation, persistent glomerular disease affecting the native kidneys typically causes albuminuria, at least for a period of time, making it difficult to determine in a noninvasive fashion whether proteinuria originates in the native kidneys or the renal allograft. To address this problem, dynamic contrast-enhanced magnetic resonance imaging (MRI) using gadolinium (Gd)-based albumin-bound blood pool contrast agent (MS325) to localize proteinuria was investigated. Glomerular proteinuria was induced in Sprague-Dawley rats by intravenous injection of puromycin aminonucleoside (PAN), whereas control rats received physiologic saline vehicle. Both groups of animals underwent a 40-min dynamic contrast-enhanced MRI using radio frequency spoiled gradient echo imaging sequence after injection of Gd-labeled MS325. Contrast uptake and clearance curves for cortex and medulla were determined from acquired MR images. Compared with controls, proteinuric rats exhibited significantly lower elimination rate constants. The use of gadopentetate dimeglumine (Gd-DTPA) as a contrast agent showed smaller and less specific differences between proteinuric and control groups. In rats with one proteinuric kidney (PAN-treated) and one normal kidney (transplanted from a normal rat), MRI using MS325 was able to differentiate between the two kidneys. The results suggest that MRI with an albumin-bound blood pool contrast agent may be a useful noninvasive way to localize proteinuria. If this technique can be successfully applied in human patients, it may allow for the localization of proteinuria after kidney transplant and thereby provide a noninvasive way to detect disease affecting the renal allograft. JF - Journal of the American Society of Nephrology : JASN AU - Zhang, Yantian AU - Choyke, Peter L AU - Lu, Huiyan AU - Takahashi, Hideko AU - Mannon, Roslyn B AU - Zhang, Xiaojie AU - Marcos, Hani AU - Li, King C P AU - Kopp, Jeffrey B AD - Department of Radiology, Warren Grant Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1752 EP - 1757 VL - 16 IS - 6 SN - 1046-6673, 1046-6673 KW - Contrast Media KW - 0 KW - Organometallic Compounds KW - Protein Synthesis Inhibitors KW - Puromycin Aminonucleoside KW - 58-60-6 KW - Gadolinium KW - AU0V1LM3JT KW - gadofosveset trisodium KW - XM33Q67UVH KW - Index Medicus KW - Rats KW - Puromycin Aminonucleoside -- adverse effects KW - Models, Animal KW - Animals KW - Protein Synthesis Inhibitors -- adverse effects KW - Male KW - Proteinuria -- diagnosis KW - Magnetic Resonance Imaging -- methods KW - Kidney Transplantation KW - Nephrosis -- complications KW - Nephrosis -- chemically induced KW - Proteinuria -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67868613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Society+of+Nephrology+%3A+JASN&rft.atitle=Detection+and+localization+of+proteinuria+by+dynamic+contrast-enhanced+magnetic+resonance+imaging+using+MS325.&rft.au=Zhang%2C+Yantian%3BChoyke%2C+Peter+L%3BLu%2C+Huiyan%3BTakahashi%2C+Hideko%3BMannon%2C+Roslyn+B%3BZhang%2C+Xiaojie%3BMarcos%2C+Hani%3BLi%2C+King+C+P%3BKopp%2C+Jeffrey+B&rft.aulast=Zhang&rft.aufirst=Yantian&rft.date=2005-06-01&rft.volume=16&rft.issue=6&rft.spage=1752&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Society+of+Nephrology+%3A+JASN&rft.issn=10466673&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-21 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ligand-selective targeting of the glucocorticoid receptor to nuclear subdomains is associated with decreased receptor mobility. AN - 67868292; 15705660 AB - The association between nuclear distribution and mobility of the human glucocorticoid receptor was examined in living COS-1 cells using yellow fluorescent protein- and cyan fluorescent protein-tagged receptors. Quantitation of the nuclear distribution induced by an array of glucocorticoid ligands revealed a continuum from a random (cortisone) to a nonrandom (triamcinolone acetonide) receptor distribution. Structure-function analysis revealed that the 9-fluoro and 17-hydroxy groups on the steroid significantly impact nuclear receptor distribution. Using time-lapse microscopy, the triamcinolone acetonide-induced receptor distribution did not change significantly over a period of 15 sec. However, using fluorescence recovery after photobleaching, the individual receptors moved at a much faster rate, indicating rapid exchange of receptors on immobile nuclear subdomains. Receptor mobilities for 13 different steroids, measured by fluorescence recovery after photobleaching, appeared to correlate with receptor distribution. Ligands that induced a nonrandom distribution induced slower receptor mobility and vice versa. Finally, application of 2-photon confocal microscopy revealed differences in receptor mobility between nuclear subdomains. Areas of high receptor concentration showed slower mobility than areas of low receptor concentration. Thus, glucocorticoid receptors can be targeted (depending on the ligand) to relatively immobile nuclear subdomains. The transient association of receptor with these domains decreases the mobility of the receptor. JF - Molecular endocrinology (Baltimore, Md.) AU - Schaaf, Marcel J M AU - Lewis-Tuffin, Laura J AU - Cidlowski, John A AD - Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA. schaaf@rulbim.leidenuniv.nl Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1501 EP - 1515 VL - 19 IS - 6 SN - 0888-8809, 0888-8809 KW - Bacterial Proteins KW - 0 KW - Cyan Fluorescent Protein KW - Ligands KW - Luminescent Proteins KW - Receptors, Glucocorticoid KW - Steroids KW - hydrocortisone receptor KW - triamcinolone acetonide receptor KW - yellow fluorescent protein, Bacteria KW - Green Fluorescent Proteins KW - 147336-22-9 KW - Triamcinolone Acetonide KW - F446C597KA KW - Cortisone KW - V27W9254FZ KW - Index Medicus KW - Microscopy, Confocal KW - Animals KW - Software KW - COS Cells KW - Photons KW - Cell Nucleus -- metabolism KW - Humans KW - Mutagenesis KW - Cortisone -- pharmacology KW - Fluorescence Recovery After Photobleaching KW - Binding, Competitive KW - Steroids -- chemistry KW - Green Fluorescent Proteins -- metabolism KW - Time Factors KW - Plasmids -- metabolism KW - Dose-Response Relationship, Drug KW - Bacterial Proteins -- metabolism KW - Luminescent Proteins -- metabolism KW - Protein Binding KW - Structure-Activity Relationship KW - Transfection KW - Triamcinolone Acetonide -- pharmacology KW - Protein Structure, Tertiary KW - Image Processing, Computer-Assisted KW - Mutation KW - Receptors, Glucocorticoid -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67868292?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+endocrinology+%28Baltimore%2C+Md.%29&rft.atitle=Ligand-selective+targeting+of+the+glucocorticoid+receptor+to+nuclear+subdomains+is+associated+with+decreased+receptor+mobility.&rft.au=Schaaf%2C+Marcel+J+M%3BLewis-Tuffin%2C+Laura+J%3BCidlowski%2C+John+A&rft.aulast=Schaaf&rft.aufirst=Marcel+J&rft.date=2005-06-01&rft.volume=19&rft.issue=6&rft.spage=1501&rft.isbn=&rft.btitle=&rft.title=Molecular+endocrinology+%28Baltimore%2C+Md.%29&rft.issn=08888809&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-14 N1 - Date created - 2005-05-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Endocannabinoid release from midbrain dopamine neurons: a potential substrate for cannabinoid receptor antagonist treatment of addiction. AN - 67864223; 15878779 AB - Substantial evidence suggests that all commonly abused drugs act upon the brain reward circuitry to ultimately increase extracellular concentrations of the neurotransmitter dopamine in the nucleus accumbens and other forebrain areas. Many drugs of abuse appear to increase dopamine levels by dramatically increase the firing and bursting rates of dopamine neurons located in the ventral mesencephalon. Recent clinical evidence in humans and behavioral evidence in animals indicate that cannabinoid receptor antagonists such as SR141716A (Rimonabant) can reduce the self-administration of, and craving for, several commonly addictive drugs. However, the mechanism of this potentially beneficial effect has not yet been identified. We propose, on the basis of recent studies in our laboratory and others, that these antagonists may act by blocking the effects of endogenously released cannabinoid molecules (endocannabinoids) that are released in an activity- and calcium-dependent manner from mesencephalic dopamine neurons. It is hypothesized that, through the antagonism of cannabinoid CB1 receptors located on inhibitory and excitatory axon terminals targeting the midbrain dopamine neurons, the effects of the endocannabinoids are occluded. The data from these studies therefore suggest that the endocannabinoid system and the CB1 receptors located in the ventral mesencephalon may play an important role in regulating drug reward processes, and that this substrate is recruited whenever dopamine neuron activity is increased. JF - Neuropharmacology AU - Lupica, Carl R AU - Riegel, Arthur C AD - Cellular Neurobiology Branch, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, U.S. Department of Health and Human Services, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. clupica@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1105 EP - 1116 VL - 48 IS - 8 KW - Cannabinoid Receptor Antagonists KW - 0 KW - Cannabinoid Receptor Modulators KW - Endocannabinoids KW - Piperidines KW - Pyrazoles KW - Dronabinol KW - 7J8897W37S KW - rimonabant KW - RML78EN3XE KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Animals KW - Reward KW - Humans KW - Dronabinol -- pharmacology KW - Ventral Tegmental Area -- metabolism KW - Ventral Tegmental Area -- drug effects KW - Signal Transduction KW - Mesencephalon -- metabolism KW - Mesencephalon -- drug effects KW - Cannabinoid Receptor Modulators -- physiology KW - Piperidines -- therapeutic use KW - Neurons -- metabolism KW - Cannabinoid Receptor Modulators -- metabolism KW - Substance-Related Disorders -- drug therapy KW - Dopamine -- metabolism KW - Pyrazoles -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67864223?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropharmacology&rft.atitle=Endocannabinoid+release+from+midbrain+dopamine+neurons%3A+a+potential+substrate+for+cannabinoid+receptor+antagonist+treatment+of+addiction.&rft.au=Lupica%2C+Carl+R%3BRiegel%2C+Arthur+C&rft.aulast=Lupica&rft.aufirst=Carl&rft.date=2005-06-01&rft.volume=48&rft.issue=8&rft.spage=1105&rft.isbn=&rft.btitle=&rft.title=Neuropharmacology&rft.issn=1873-7064&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2009-07-17 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hematopoietic stem cell transplantation prevents diabetes in NOD mice but does not contribute to significant islet cell regeneration once disease is established. AN - 67853510; 15911094 AB - The treatment of type I diabetes by islet cell transplantation, while promising, remains restricted due to the incomplete efficacy and toxicity associated with current immunosuppression, and by limited organ availability. Given reports suggesting bone marrow derived stem cell plasticity, we sought to determine whether such cells could give rise to pancreatic islet cells in vivo. In the context of autoimmune diabetes, we transplanted unfractionated bone marrow from beta-gal trangenic donor mice into NOD mice prior to, at, and two weeks beyond the onset of disease. Successful bone marrow engraftment before diabetes onset prevented disease in all mice and for 1 year after transplant. However, despite obtaining full hematopoietic engraftment in over 50 transplanted mice, only one mouse became insulin independent, and no beta-Gal positive islets were detected in any of the mice. To test whether tolerance to islets was achieved, we injected islets obtained from the same allogeneic donor strain as the hematopoietic cells into 4 transplant recipients, and 2 had a reversion of their diabetes. Thus allogeneic bone marrow transplantation prevents autoimmune diabetes and tolerizes the recipient to donor islet grants, even in diabetic animals, yet the capacity of bone marrow derived cells to differentiate into functional islet cells, at least without additional manipulation, is limited in our model. JF - Experimental hematology AU - Kang, Elizabeth M AU - Zickler, Philipp P AU - Burns, Sean AU - Langemeijer, Saskia M AU - Brenner, Sebastian AU - Phang, Oswald A AU - Patterson, Noelle AU - Harlan, David AU - Tisdale, John F AD - LHD/NIAID, National Institutes of Health/DHHS, Bethesda, MD 20895, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 699 EP - 705 VL - 33 IS - 6 SN - 0301-472X, 0301-472X KW - Index Medicus KW - Animals KW - Mice, Inbred NOD KW - Mice, Inbred C57BL KW - Mice KW - Islets of Langerhans -- physiopathology KW - Diabetes Mellitus, Type 1 -- prevention & control KW - Regeneration KW - Hematopoietic Stem Cell Transplantation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67853510?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+hematology&rft.atitle=Hematopoietic+stem+cell+transplantation+prevents+diabetes+in+NOD+mice+but+does+not+contribute+to+significant+islet+cell+regeneration+once+disease+is+established.&rft.au=Kang%2C+Elizabeth+M%3BZickler%2C+Philipp+P%3BBurns%2C+Sean%3BLangemeijer%2C+Saskia+M%3BBrenner%2C+Sebastian%3BPhang%2C+Oswald+A%3BPatterson%2C+Noelle%3BHarlan%2C+David%3BTisdale%2C+John+F&rft.aulast=Kang&rft.aufirst=Elizabeth&rft.date=2005-06-01&rft.volume=33&rft.issue=6&rft.spage=699&rft.isbn=&rft.btitle=&rft.title=Experimental+hematology&rft.issn=0301472X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of beta-leptinotarsin-h and the effects of calcium flux antagonists on its activity. AN - 67851645; 15904678 AB - beta-Leptinotarsin-h, purified from the hemolymph of the beetle Leptinotarsa haldemani, is a potent ( approximately 1 nM) neuroactive protein that rapidly (few seconds) stimulates Ca(2+) influx and neurotransmitter release. Our goals were to further characterize beta-leptinotarsin-h and to test the hypothesis that it stimulates Ca(2+) influx through presynaptic Ca(2+) channels. Analysis of partial amino acid sequences revealed that beta-leptinotarsin-h is a unique protein with significant similarity to only one other protein, the juvenile hormone esterase of Leptinotarsa decemlineata, commonly known as the Colorado potato beetle. We have examined the effect of beta-leptinotarsin-h on Ca(2+) current, Ca(2+) uptake, Ca(2+) levels, and neurotransmitter release in synaptosomes, cell lines, and neuronal systems. We found that its preferred site of action appears to be mammalian presynaptic nerve terminals. We tested antagonists of Ca(2+) flux for their effects on beta-leptinotarsin-h-stimulated Ca(2+) uptake in rat brain synaptosomes. The non-selective Ca(2+) channel blockers flunarizine, Ni(2+), ruthenium red, high-concentration thapsigargin, and SKF 96365 inhibited beta-leptinotarsin-h's activity, but none of the tested selective blockers of voltage-operated Ca(2+) channels (omega-agatoxin IVA, omega-conotoxin GVIA, omega-conotoxin MVIIC, nicardipine, nifedipine, SNX-482) was inhibitory. Selective inhibitors of ligand-operated, store-operated, and transduction-operated channels were also not inhibitory. beta-Leptinotarsin-h did not stimulate Na(+) uptake, ruling out Na(+) channels and many non-selective cation channels as targets. We conclude that beta-leptinotarsin-h stimulated Ca(2+) uptake through presynaptic Ca(2+) channels; which channel is yet to be determined. beta-Leptinotarsin-h may prove to be a useful tool with which to investigate calcium channels and calcium flux. JF - Toxicon : official journal of the International Society on Toxinology AU - Crosland, Richard D AU - Fitch, Richard W AU - Hines, Harry B AD - Scientific Review Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-9529, USA. crosland@nih.gov Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 829 EP - 841 VL - 45 IS - 7 SN - 0041-0101, 0041-0101 KW - Antibodies, Monoclonal KW - 0 KW - Calcium Channel Agonists KW - Calcium Channel Blockers KW - Calcium Channels KW - Insect Proteins KW - Neurotoxins KW - Neurotransmitter Agents KW - leptinotarsin protein, Leptinotarsa haldemani KW - 73468-57-2 KW - Sodium KW - 9NEZ333N27 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Synaptosomes -- drug effects KW - Amino Acid Sequence KW - Mice KW - Mice, Inbred BALB C KW - Presynaptic Terminals -- metabolism KW - Rats KW - Presynaptic Terminals -- drug effects KW - Rats, Sprague-Dawley KW - Synaptosomes -- secretion KW - Calcium Channel Blockers -- pharmacology KW - Neurotransmitter Agents -- secretion KW - Sodium -- metabolism KW - Calcium -- metabolism KW - Insect Proteins -- isolation & purification KW - Calcium Channels -- metabolism KW - Neurotoxins -- pharmacology KW - Insect Proteins -- pharmacology KW - Calcium Channel Agonists -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67851645?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicon+%3A+official+journal+of+the+International+Society+on+Toxinology&rft.atitle=Characterization+of+beta-leptinotarsin-h+and+the+effects+of+calcium+flux+antagonists+on+its+activity.&rft.au=Crosland%2C+Richard+D%3BFitch%2C+Richard+W%3BHines%2C+Harry+B&rft.aulast=Crosland&rft.aufirst=Richard&rft.date=2005-06-01&rft.volume=45&rft.issue=7&rft.spage=829&rft.isbn=&rft.btitle=&rft.title=Toxicon+%3A+official+journal+of+the+International+Society+on+Toxinology&rft.issn=00410101&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-16 N1 - Date created - 2005-05-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Additive effects of endogenous cannabinoid anandamide and ethanol on alpha7-nicotinic acetylcholine receptor-mediated responses in Xenopus Oocytes. AN - 67845663; 15687372 AB - The interaction between the effects of the endogenous cannabinoid receptor agonist anandamide and ethanol on the function of homomeric alpha(7)-nicotinic acetylcholine (nACh) receptors expressed in Xenopus oocytes were investigated using the two-electrode voltage-clamp technique. Anandamide and ethanol reversibly inhibited currents evoked with 100 microM acetylcholine in a concentration-dependent manner. Coapplication of anandamide and ethanol caused a significantly greater inhibition of alpha(7)-nACh receptor function than anandamide or ethanol alone. The IC(50) value of 238 +/- 34 nM for anandamide inhibition decreased significantly to 104 +/- 23 nM in the presence of 30 mM ethanol. The inhibition of alpha(7)-mediated currents by coapplication of anandamide and ethanol was not altered by phenylmethylsulfonyl fluoride, an inhibitor of anandamide hydrolyzing enzyme, or N-(4-hydroxyphenyl)-arachidonylamide, an anandamide transport inhibitor. Analysis of oocytes by matrix-assisted laser desorption/ionization technique indicated that ethanol treatment did not alter the lipid profile of oocytes, and there is negligible, if any, anandamide present in these cells. Results of studies with chimeric alpha(7)-nACh-5-HT(3) receptors comprised of the amino-terminal domain of the alpha(7)-nACh receptor and the transmembrane and carboxyl-terminal domains of 5-HT(3) receptors suggest that although ethanol inhibition of the alpha(7)-nACh receptor is likely to involve the N-terminal region of the receptor, the site of action for anandamide is located in the transmembrane and carboxyl-terminal domains of the receptors. These data indicate that endocannabinoids and ethanol potentiate each other's inhibitory effects on alpha(7)-nACh receptor function through distinct regions of the receptor. JF - The Journal of pharmacology and experimental therapeutics AU - Oz, Murat AU - Jackson, Shelley N AU - Woods, Amina S AU - Morales, Marisela AU - Zhang, Li AD - National Institute on Drug Abuse/Intramural Research Program, Cellular Neurobiology Branch, Baltimore, MD 21224, USA. moz@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1272 EP - 1280 VL - 313 IS - 3 SN - 0022-3565, 0022-3565 KW - Arachidonic Acids KW - 0 KW - Chloride Channels KW - Endocannabinoids KW - Nicotinic Antagonists KW - Polyunsaturated Alkamides KW - Receptors, Nicotinic KW - alpha7 Nicotinic Acetylcholine Receptor KW - Ethanol KW - 3K9958V90M KW - Acetylcholine KW - N9YNS0M02X KW - Calcium KW - SY7Q814VUP KW - anandamide KW - UR5G69TJKH KW - Index Medicus KW - Calcium -- metabolism KW - Xenopus laevis KW - Animals KW - Dose-Response Relationship, Drug KW - Oocytes KW - Chloride Channels -- drug effects KW - Acetylcholine -- pharmacology KW - Drug Synergism KW - Female KW - Ethanol -- pharmacology KW - Nicotinic Antagonists -- pharmacology KW - Receptors, Nicotinic -- drug effects KW - Receptors, Nicotinic -- physiology KW - Arachidonic Acids -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67845663?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Additive+effects+of+endogenous+cannabinoid+anandamide+and+ethanol+on+alpha7-nicotinic+acetylcholine+receptor-mediated+responses+in+Xenopus+Oocytes.&rft.au=Oz%2C+Murat%3BJackson%2C+Shelley+N%3BWoods%2C+Amina+S%3BMorales%2C+Marisela%3BZhang%2C+Li&rft.aulast=Oz&rft.aufirst=Murat&rft.date=2005-06-01&rft.volume=313&rft.issue=3&rft.spage=1272&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Changes in androgen-mediated reproductive development in male rat offspring following exposure to a single oral dose of flutamide at different gestational ages. AN - 67843129; 15788718 AB - Previous studies have indicated that the androgen receptor antagonist, flutamide, can produce a suite of reproductive malformations in the male rat when orally administered daily on gestation days (GD) 12-21. The objective of this study was to investigate the gestation time dependence for the induction of these malformations to establish a robust animal model for future studies of gene expression related to specific malformations. Groups of timed-pregnant Sprague-Dawley rats (GD 0 = day of mating) were administered flutamide as a single gavage dose (50 mg/kg) on GD 16, 17, 18, or 19 with 10 dams per group. Control animals (5 dams per time per group) were administered corn oil vehicle (2 ml/kg). Dams were allowed to litter, and their adult male offspring were killed at postnatal day (PND) 100 +/- 10. Anogenital distance was measured at PND 1 and 100. Areolae were scored at PND 13, and permanent nipples evaluated at PND 100. No reproductive tract malformations were found in control male offspring. In the treated groups, malformations were noted following exposure at every GD, although the incidence of specific malformations varied by GD. At GD 16, the highest incidence was noted for permanent nipples (46% pups, 60% litters), epispadias (12% pups, 30% litters), and missing epididymal components (5% pups, 20% litters). The highest incidences for hypospadias (58% pups, 80% litters), vaginal pouch (49% pups, 70% litters), cleft prepuce (29% pups, 60% litters), and missing prostate lobes (12% pups, 60% litters) were noted at GD 17. At GD 18 the highest incidence of malformations noted were epispadias (5% pups, 30% litters), reduced prostate size (32% pups, 90% litters), and abnormal kidneys (3% pups, 30% litters) and bladders (7% pups, 30% litters), while on GD 19 70% of the litters had animals with abnormal seminal vesicles. Testicular and epididymal morphological changes were noted at all GDs and were consistent with the gross observations and peaked in incidence and severity on GD17. The major discrepancy between this study and previous multiple-dose studies was in the very few numbers of animals presenting with cryptorchidism (only one each on GDs 16 and 17), suggesting that exposure over multiple days may be required to induce this malformation. Thus, a single gestational exposure of flutamide induced numerous reproductive tract malformations consistent with previously reports following multiple exposures, with the timing of the exposure producing marked tissue selectivity in the response noted in adult offspring. JF - Toxicological sciences : an official journal of the Society of Toxicology AU - Foster, Paul M D AU - Harris, Martha W AD - Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. foster2@niehs.nih.gov Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1024 EP - 1032 VL - 85 IS - 2 SN - 1096-6080, 1096-6080 KW - Androgen Antagonists KW - 0 KW - Androgens KW - Flutamide KW - 76W6J0943E KW - Index Medicus KW - Animals KW - Testis -- abnormalities KW - Epididymis -- pathology KW - Testis -- pathology KW - Gestational Age KW - Cryptorchidism -- pathology KW - Pregnancy KW - Cryptorchidism -- chemically induced KW - Rats KW - Epididymis -- abnormalities KW - Female KW - Male KW - Prenatal Exposure Delayed Effects KW - Androgen Antagonists -- toxicity KW - Genitalia, Male -- drug effects KW - Androgens -- physiology KW - Genitalia, Male -- abnormalities KW - Flutamide -- toxicity KW - Genitalia, Male -- growth & development UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67843129?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.atitle=Changes+in+androgen-mediated+reproductive+development+in+male+rat+offspring+following+exposure+to+a+single+oral+dose+of+flutamide+at+different+gestational+ages.&rft.au=Foster%2C+Paul+M+D%3BHarris%2C+Martha+W&rft.aulast=Foster&rft.aufirst=Paul+M&rft.date=2005-06-01&rft.volume=85&rft.issue=2&rft.spage=1024&rft.isbn=&rft.btitle=&rft.title=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-18 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Development of a rationally designed, low abuse potential, biogenic amine releaser that suppresses cocaine self-administration. AN - 67842394; 15761112 AB - Convergent lines of evidence support a dual deficit model of stimulant withdrawal, where reductions in synaptic dopamine (DA) and 5-hydroxytryptamine (serotonin) (5-HT) contribute to dysphoria, drug craving, and relapse. Thus, we predicted that a nonamphetamine compound with substrate activity at DA and 5-HT transporters (i.e., a dual DA/5-HT releaser) would be an effective medication for treating stimulant addictions. Ideally, this type of medication would alleviate withdrawal symptoms, suppress cocaine self-administration, and lack side effects commonly associated with central nervous system stimulants. In the present work, more than 350 compounds were screened in vitro for activity as substrate-type releasing agents at DA, 5-HT, and norepinephrine transporters. These efforts identified PAL-287 (1-napthyl-2-aminopropane) as a nonamphetamine compound with potent substrate activity at biogenic amine transporters. In vivo microdialysis in rats demonstrated that PAL-287 (1-3 mg/kg i.v.) increased extracellular DA and 5-HT in frontal cortex, but effects on 5-HT were somewhat greater. PAL-287 induced substantially less locomotor stimulation than (+)-amphetamine, a drug that increases only extracellular DA. Administration of high-dose (+)-methamphetamine or (+/-)-3,4-methylenedioxymethamphetamine to rats produced long-lasting depletion of cortical 5-HT, whereas PAL-287 (18 mg/kg i.p. x 3) did not. PAL-287 displayed little or no reinforcing properties in rhesus monkeys trained to self-administer cocaine, yet PAL-287 produced a dose-dependent decrease in responding for cocaine when infused at a dose of 1.0 mg/kg/h. Collectively, the findings reported here demonstrate that nonamphetamine monoamine releasing agents such as PAL-287 might be promising candidate medications for the treatment of stimulant dependence. JF - The Journal of pharmacology and experimental therapeutics AU - Rothman, Richard B AU - Blough, Bruce E AU - Woolverton, William L AU - Anderson, Karen G AU - Negus, S Stevens AU - Mello, Nancy K AU - Roth, Bryan L AU - Baumann, Michael H AD - Clinical Psychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1361 EP - 1369 VL - 313 IS - 3 SN - 0022-3565, 0022-3565 KW - Dopamine Plasma Membrane Transport Proteins KW - 0 KW - Membrane Glycoproteins KW - Membrane Transport Proteins KW - Nerve Tissue Proteins KW - Serotonin Plasma Membrane Transport Proteins KW - Slc6a4 protein, rat KW - Serotonin KW - 333DO1RDJY KW - Cocaine KW - I5Y540LHVR KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats KW - Nerve Tissue Proteins -- physiology KW - Animals KW - Rats, Sprague-Dawley KW - Self Administration KW - Membrane Glycoproteins -- physiology KW - Membrane Transport Proteins -- physiology KW - Macaca mulatta KW - Male KW - Dopamine -- secretion KW - Serotonin -- secretion KW - Cocaine -- administration & dosage KW - Cocaine-Related Disorders -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67842394?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Development+of+a+rationally+designed%2C+low+abuse+potential%2C+biogenic+amine+releaser+that+suppresses+cocaine+self-administration.&rft.au=Rothman%2C+Richard+B%3BBlough%2C+Bruce+E%3BWoolverton%2C+William+L%3BAnderson%2C+Karen+G%3BNegus%2C+S+Stevens%3BMello%2C+Nancy+K%3BRoth%2C+Bryan+L%3BBaumann%2C+Michael+H&rft.aulast=Rothman&rft.aufirst=Richard&rft.date=2005-06-01&rft.volume=313&rft.issue=3&rft.spage=1361&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chromosomal abnormalities in bronchial epithelium from smokers, nonsmokers, and lung cancer patients. AN - 67840300; 15899385 AB - The identification of individuals who are at greatest risk of developing lung cancer would greatly improve diagnosis and possibly lead to early treatment. To study the use of karyotypes for this purpose, we used short-term human bronchial epithelial (hBE) cell cultures from nonsmokers, smokers, and lung cancer patients. Twenty-five metaphases were scored for hBE cell cultures obtained from 32 patients: 8 were nonsmokers, and 24 had a history of smoking (of whom 11 had had lung cancer surgery). The number of abnormal metaphases ranged from 0 to 4 per cell culture. No overall differences in the number of abnormal metaphases were observed between nonsmokers and smokers or between lung cancer patients and non-lung cancer patients. The most commonly observed abnormalities were structural changes in chromosome 1 (six cultures), loss of chromosome 17 (six cultures), and trisomy of chromosome 20 (three cultures). These specific alterations were found almost exclusively in patients with a history of tobacco smoking. The results did not indicate that general chromosomal abnormalities are a useful marker for tobacco smoke exposure or cancer risk. JF - Cancer genetics and cytogenetics AU - Slebos, Robbert J C AU - Livanos, Elizabeth AU - Yim, Hyeon-Woo AU - Randell, Scott H AU - Parsons, Alden M AU - Detterbeck, Frank C AU - Rivera, M Patricia AU - Taylor, Jack A AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 137 EP - 142 VL - 159 IS - 2 SN - 0165-4608, 0165-4608 KW - Index Medicus KW - Karyotyping KW - Tumor Cells, Cultured KW - Epithelial Cells KW - Bronchi -- cytology KW - Cells, Cultured KW - Humans KW - Adult KW - Middle Aged KW - Child KW - Adolescent KW - Male KW - Female KW - Lung Neoplasms -- diagnosis KW - Chromosome Aberrations KW - Lung Neoplasms -- genetics KW - Smoking -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67840300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+genetics+and+cytogenetics&rft.atitle=Chromosomal+abnormalities+in+bronchial+epithelium+from+smokers%2C+nonsmokers%2C+and+lung+cancer+patients.&rft.au=Slebos%2C+Robbert+J+C%3BLivanos%2C+Elizabeth%3BYim%2C+Hyeon-Woo%3BRandell%2C+Scott+H%3BParsons%2C+Alden+M%3BDetterbeck%2C+Frank+C%3BRivera%2C+M+Patricia%3BTaylor%2C+Jack+A&rft.aulast=Slebos&rft.aufirst=Robbert+J&rft.date=2005-06-01&rft.volume=159&rft.issue=2&rft.spage=137&rft.isbn=&rft.btitle=&rft.title=Cancer+genetics+and+cytogenetics&rft.issn=01654608&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Black tea polyphenols suppress cell proliferation and induce apoptosis during benzo(a)pyrene-induced lung carcinogenesis. AN - 67838727; 15901989 AB - One of the most promising strategies for cancer prevention is chemoprevention by daily used food and beverages. Black tea, the most widely consumed beverage, is a source of compounds with antioxidative, antimicrobial, antimutagenic and anticarcinogenic properties. Lung cancer is the most common cause of cancer deaths in both men and women worldwide. Over one million people around the world are likely to be killed by lung cancer due to increased tobacco smoking and environmental pollutants, especially car exhausts. Therefore chemopreventive intervention using black tea and its active components may be a viable means to reduce lung cancer death. In the present investigation, we used benzo(a)pyrene (BP) to induce lung carcinogenesis in mice for the assessment of potential apoptosis-inducing and proliferation-suppressing effects of theaflavins and epigallocatechin gallate, active components of black tea. Hyperplasia, dysplasia and carcinoma in situ evident in the carcinogen control group on the 8th, 17th and 26th weeks respectively, were effectively reduced after treatment with theaflavins and epigallocatechin gallate. Significant reduction in number of proliferating cells and increased number of apoptotic cells was also found on the 8th, 17th and 26th week of treatment with theaflavins and epigallocatechin gallate in BP-exposed mice. Our observation suggests a promising role for black tea polyphenols in the prevention of lung cancer. JF - European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) AU - Banerjee, S AU - Manna, S AU - Saha, P AU - Panda, C Kr AU - Das, S AD - Department of Cancer Chemoprevention, Chittarajan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, West Bengal, India. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 215 EP - 221 VL - 14 IS - 3 SN - 0959-8278, 0959-8278 KW - Biflavonoids KW - 0 KW - Tea KW - theaflavin KW - 1IA46M0D13 KW - Benzo(a)pyrene KW - 3417WMA06D KW - Catechin KW - 8R1V1STN48 KW - epigallocatechin gallate KW - BQM438CTEL KW - Index Medicus KW - Animals KW - Benzo(a)pyrene -- toxicity KW - Apoptosis -- drug effects KW - Mice KW - Carcinoma in Situ -- prevention & control KW - Carcinoma in Situ -- physiopathology KW - Cell Proliferation -- drug effects KW - Lung Neoplasms -- veterinary KW - Lung Neoplasms -- prevention & control KW - Catechin -- analogs & derivatives KW - Biflavonoids -- pharmacology KW - Tea -- chemistry KW - Catechin -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67838727?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=European+journal+of+cancer+prevention+%3A+the+official+journal+of+the+European+Cancer+Prevention+Organisation+%28ECP%29&rft.atitle=Black+tea+polyphenols+suppress+cell+proliferation+and+induce+apoptosis+during+benzo%28a%29pyrene-induced+lung+carcinogenesis.&rft.au=Banerjee%2C+S%3BManna%2C+S%3BSaha%2C+P%3BPanda%2C+C+Kr%3BDas%2C+S&rft.aulast=Banerjee&rft.aufirst=S&rft.date=2005-06-01&rft.volume=14&rft.issue=3&rft.spage=215&rft.isbn=&rft.btitle=&rft.title=European+journal+of+cancer+prevention+%3A+the+official+journal+of+the+European+Cancer+Prevention+Organisation+%28ECP%29&rft.issn=09598278&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-19 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thr176 regulates the activity of the mouse nuclear receptor CAR and is conserved in the NR1I subfamily members PXR and VDR. AN - 67838656; 15610065 AB - The mouse nuclear receptor CAR (constitutively active receptor) is a transcription factor that is activated by phenobarbital-type inducers such as TCPOBOP {1,4 bis[2-(3,5-dichloropyridyloxy)]benzene} in liver in vivo. However, CAR is constitutively active in cell-based transfection assays, the molecular mechanism for which has not been elucidated yet. In the model structure of CAR, Thr176 constitutes a part of the ligand-binding surface, but its side chain is not directed toward the surface, instead it forms a hydrogen bond with Thr350 in the AF2 (activation function 2) domain of CAR. Thr350 is known to regulate CAR activity [Ueda, Kakizaki, Negishi, and Sueyoshi (2002) Mol. Pharmacol. 61, 1284-1288]. Thr176 was mutated to various amino acids to examine whether this interaction played a role in conferring the constitutive activity. Hydrophobic and positively charged amino acids at position 176 abrogated the constitutive activity, whereas polar and negatively charged amino acids retained it. When one of the small hydrophobic amino acids, such as alanine or valine, was substituted for threonine, the mutants were fully activated by TCPOBOP. The co-activator SRC-1 (steroid receptor co-activator-1) regulated the activity changes associated with the mutations. Thr248 and Ser230 are the Thr176-corresponding residues in human pregnane X receptor and mouse vitamin D3 receptor respectively, interacting directly with the conserved threonine in the AF2 domains. Thr248 and Ser230 also regulated the ligand-dependent activity of these receptors by augmenting binding of the receptors to SRC-1. Thr176, Thr248 and Ser230 are conserved residues in the NR1I (nuclear receptor 1I) subfamily members and determine their activity. JF - The Biochemical journal AU - Ueda, Akiko AU - Matsui, Kenji AU - Yamamoto, Yukio AU - Pedersen, Lars C AU - Sueyoshi, Tatsuya AU - Negishi, Masahiko AD - Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 623 EP - 630 VL - 388 KW - Pyridines KW - 0 KW - Receptors, Calcitriol KW - Receptors, Cytoplasmic and Nuclear KW - Receptors, Steroid KW - Transcription Factors KW - constitutive androstane receptor KW - pregnane X receptor KW - Threonine KW - 2ZD004190S KW - 1,4-bis(2-(3,5-dichloropyridyloxy))benzene KW - 76150-91-9 KW - Histone Acetyltransferases KW - EC 2.3.1.48 KW - NCOA1 protein, human KW - Ncoa1 protein, mouse KW - Nuclear Receptor Coactivator 1 KW - Index Medicus KW - Animals KW - Humans KW - Amino Acid Sequence KW - Mice KW - Protein Binding KW - Mutagenesis, Site-Directed KW - Sequence Alignment KW - Conserved Sequence KW - Molecular Sequence Data KW - Gene Expression Regulation KW - Cell Line KW - Receptors, Cytoplasmic and Nuclear -- physiology KW - Receptors, Calcitriol -- physiology KW - Transcription Factors -- physiology KW - Threonine -- chemistry KW - Receptors, Steroid -- physiology KW - Receptors, Steroid -- chemistry KW - Receptors, Cytoplasmic and Nuclear -- chemistry KW - Transcription Factors -- chemistry KW - Receptors, Calcitriol -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67838656?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Biochemical+journal&rft.atitle=Thr176+regulates+the+activity+of+the+mouse+nuclear+receptor+CAR+and+is+conserved+in+the+NR1I+subfamily+members+PXR+and+VDR.&rft.au=Ueda%2C+Akiko%3BMatsui%2C+Kenji%3BYamamoto%2C+Yukio%3BPedersen%2C+Lars+C%3BSueyoshi%2C+Tatsuya%3BNegishi%2C+Masahiko&rft.aulast=Ueda&rft.aufirst=Akiko&rft.date=2005-06-01&rft.volume=388&rft.issue=&rft.spage=623&rft.isbn=&rft.btitle=&rft.title=The+Biochemical+journal&rft.issn=1470-8728&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-24 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Cell Biol. 1999 Sep;19(9):6318-22 [10454578] J Biol Chem. 1999 Mar 5;274(10):6043-6 [10037683] Mol Pharmacol. 1999 Dec;56(6):1329-39 [10570062] Genes Dev. 2000 Jan 15;14(2):121-41 [10652267] Nature. 2000 Oct 19;407(6806):920-3 [11057673] Mol Endocrinol. 2000 Nov;14(11):1897-905 [11075820] Annu Rev Pharmacol Toxicol. 2001;41:123-43 [11264453] Hepatology. 2001 May;33(5):1232-8 [11343253] Proc Natl Acad Sci U S A. 2001 May 8;98(10):5491-6 [11344298] Science. 2001 Jun 22;292(5525):2329-33 [11408620] Mol Pharmacol. 2002 Jan;61(1):1-6 [11752199] Mol Pharmacol. 2002 Jun;61(6):1284-8 [12021388] Arch Biochem Biophys. 2003 Jan 1;409(1):207-11 [12464260] Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4156-61 [12644704] Biochem Biophys Res Commun. 2004 Mar 19;315(4):919-27 [14985100] J Biol Chem. 2004 May 7;279(19):19832-8 [15004031] Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 [6828386] Acta Crystallogr A. 1991 Mar 1;47 ( Pt 2):110-9 [2025413] J Clin Invest. 1998 Sep 1;102(5):1016-23 [9727070] Mol Cell Biol. 1998 Oct;18(10):5652-8 [9742082] Nature. 1998 Sep 10;395(6698):137-43 [9744270] Nature. 1998 Oct 8;395(6702):612-5 [9783588] Cell. 1998 Dec 23;95(7):927-37 [9875847] Endocr Rev. 1999 Oct;20(5):689-725 [10529899] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characteristics of self-cutters among male inmates: association with bulimia and dissociation. AN - 67834434; 15896226 AB - It was examined whether bulimia and dissociation are common in male self-cutters, as has been found in female self-cutters. The subjects were 796 male inmates of a juvenile prison. A self-reporting questionnaire was used to assess self-cutting, histories of psychoactive substance use, problem behaviors, and traumatic life events in the subjects. The Adolescent Dissociative Experience Scale and the Bulimia Investigatory Test of Edinburgh were also used. Subjects were divided into two groups: self-cutting and non-cutting. Questionnaire responses and dissociation and bulimia assessments were compared between the groups. Self-cutters began smoking (P < 0.001) and drinking (P < 0.001) earlier, and more frequently used illicit psychoactive drugs (P < 0.001), experienced childhood physical abuse (P < 0.001), and reported suicide attempts (P < 0.001), suicidal ideation (P < 0.001), and outward violence toward a person (P < 0.001) or object (P < 0.001) than non-cutters. Self-cutters also scored significantly higher on the bulimia (P < 0.001) and dissociation tests (P < 0.001). Logistic regression analysis demonstrated that suicide attempt (odds ratio, 4.311) and suicidal ideation (odds ratio, 2.336) could discriminate between male inmates with and without self-cutting. Male self-cutters showed 'multi-impulsive bulimic' tendencies resembling those of female self-cutters, although to a lesser extent. Clinical features of male as opposed to female self-cutters were influenced by gender differences. JF - Psychiatry and clinical neurosciences AU - Matsumoto, Toshihiko AU - Yamaguchi, Akiko AU - Asami, Takeshi AU - Okada, Takayuki AU - Yoshikawa, Kazuo AU - Hirayasu, Yoshio AD - Division of Forensic Psychiatry, National Institute of Mental Health, National Center of Neurology and Psychiatry, Ichikawa, Japan. tmatsu@ncnp-k.go.jp Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 319 EP - 326 VL - 59 IS - 3 SN - 1323-1316, 1323-1316 KW - Index Medicus KW - Child Abuse -- psychology KW - Suicide, Attempted -- psychology KW - Logistic Models KW - Humans KW - Adult KW - Surveys and Questionnaires KW - Child KW - Substance-Related Disorders -- psychology KW - Adolescent KW - Violence -- psychology KW - Male KW - Substance-Related Disorders -- epidemiology KW - Bulimia -- psychology KW - Dissociative Disorders -- psychology KW - Self Mutilation -- complications KW - Bulimia -- complications KW - Self Mutilation -- psychology KW - Prisoners -- psychology KW - Dissociative Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67834434?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychiatry+and+clinical+neurosciences&rft.atitle=Characteristics+of+self-cutters+among+male+inmates%3A+association+with+bulimia+and+dissociation.&rft.au=Matsumoto%2C+Toshihiko%3BYamaguchi%2C+Akiko%3BAsami%2C+Takeshi%3BOkada%2C+Takayuki%3BYoshikawa%2C+Kazuo%3BHirayasu%2C+Yoshio&rft.aulast=Matsumoto&rft.aufirst=Toshihiko&rft.date=2005-06-01&rft.volume=59&rft.issue=3&rft.spage=319&rft.isbn=&rft.btitle=&rft.title=Psychiatry+and+clinical+neurosciences&rft.issn=13231316&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-02 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Drug preferences in illicit drug abusers with a childhood tendency of attention deficit/hyperactivity disorder: a study using the Wender Utah Rating Scale in a Japanese prison. AN - 67834259; 15896225 AB - The purpose of this study is to clarify the relationship between childhood tendencies of attention deficit/hyperactivity disorder (AD/HD) and illicit drug abuse in Japanese prisoners, and to clarify whether drug abusers with AD/HD prefer methamphetamine (MAP) more than other illicit drugs. The Japanese version of the Wender Utah Rating Scale (WURS), which is a self-reporting instrument to retrospectively identify childhood tendencies of AD/HD tendencies, was carried given to 413 prisoners without a drug addiction and 282 prisoners with a drug addiction (192, MAP; 53, toluene; and 37, cannabis). WURS scores were compared between prisoners with and without a drug addiction, and between MAP, toluene, and cannabis abusers. Consequently, prisoners with a drug addiction showed significantly higher WURS scores than those without the addiction (P < 0.001). Toluene abusers showed significantly higher WURS scores than cannabis abusers (P < 0.001), and included a higher proportion with scores over cut-off than MAP or cannabis abusers (P = 0.005). In conclusion, a close relationship existed between illicit drug abuse and childhood AD/HD tendencies. Drug-abusing prisoners with AD/HD tendencies were not prone to choose MAP over other illicit drugs. JF - Psychiatry and clinical neurosciences AU - Matsumoto, Toshihiko AU - Yamaguchi, Akiko AU - Asami, Takeshi AU - Kamijo, Atsushi AU - Iseki, Eizo AU - Hirayasu, Yoshio AU - Wada, Kiyoshi AD - Division of Forensic Psychiatry, National Institute of Mental Health, National Center of Neurology and Psychiatry, Chiba, Japan. tmatsu@ncnp-k.go.jp Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 311 EP - 318 VL - 59 IS - 3 SN - 1323-1316, 1323-1316 KW - Central Nervous System Stimulants KW - 0 KW - Toluene KW - 3FPU23BG52 KW - Methamphetamine KW - 44RAL3456C KW - Index Medicus KW - Amphetamine-Related Disorders -- epidemiology KW - Age of Onset KW - Humans KW - Retrospective Studies KW - Amphetamine-Related Disorders -- psychology KW - Japan -- epidemiology KW - Psychiatric Status Rating Scales KW - Adult KW - Surveys and Questionnaires KW - Marijuana Abuse -- epidemiology KW - Marijuana Abuse -- psychology KW - Adolescent KW - Male KW - Attention Deficit Disorder with Hyperactivity -- psychology KW - Attention Deficit Disorder with Hyperactivity -- epidemiology KW - Substance-Related Disorders -- psychology KW - Prisoners -- psychology KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67834259?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychiatry+and+clinical+neurosciences&rft.atitle=Drug+preferences+in+illicit+drug+abusers+with+a+childhood+tendency+of+attention+deficit%2Fhyperactivity+disorder%3A+a+study+using+the+Wender+Utah+Rating+Scale+in+a+Japanese+prison.&rft.au=Matsumoto%2C+Toshihiko%3BYamaguchi%2C+Akiko%3BAsami%2C+Takeshi%3BKamijo%2C+Atsushi%3BIseki%2C+Eizo%3BHirayasu%2C+Yoshio%3BWada%2C+Kiyoshi&rft.aulast=Matsumoto&rft.aufirst=Toshihiko&rft.date=2005-06-01&rft.volume=59&rft.issue=3&rft.spage=311&rft.isbn=&rft.btitle=&rft.title=Psychiatry+and+clinical+neurosciences&rft.issn=13231316&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-02 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The open reading frame 3 gene of hepatitis E virus contains a cis-reactive element and encodes a protein required for infection of macaques. AN - 67823175; 15890906 AB - An infectious cDNA clone of hepatitis E virus was mutated in order to prevent synthesis of either open reading frame 2 (ORF2) protein or ORF3 protein. HuH-7 cells transfected with an ORF2-null mutant produced ORF3, and those transfected with an ORF3-null mutant produced ORF2. Silent mutations introduced into a highly conserved nucleotide sequence in the ORF3 coding region eliminated the synthesis of both ORF2 and ORF3 proteins, suggesting that it comprised a cis-reactive element. A mutant that was not able to produce ORF3 protein did not produce a detectable infection in rhesus macaques. However, a mutant that encoded an ORF3 protein lacking a phosphorylation site reported to be critical for function was able to replicate its genome in cell culture and to induce viremia and seroconversion in rhesus monkeys, suggesting that phosphorylation of ORF3 protein was not necessary for genome replication or for production of infectious virions. JF - Journal of virology AU - Graff, Judith AU - Nguyen, Hanh AU - Yu, Claro AU - Elkins, William R AU - St Claire, Marisa AU - Purcell, Robert H AU - Emerson, Suzanne U AD - Molecular Hepatitis Section, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892-8009, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 6680 EP - 6689 VL - 79 IS - 11 SN - 0022-538X, 0022-538X KW - DNA, Complementary KW - 0 KW - DNA, Viral KW - RNA, Viral KW - Viral Proteins KW - Index Medicus KW - Viral Proteins -- genetics KW - Animals KW - DNA, Complementary -- genetics KW - Open Reading Frames KW - Humans KW - Amino Acid Sequence KW - Virus Replication -- physiology KW - Nucleic Acid Conformation KW - Viral Proteins -- physiology KW - Frameshift Mutation KW - Mutagenesis, Site-Directed KW - Virus Replication -- genetics KW - Base Sequence KW - Virulence -- physiology KW - Phosphorylation KW - Transfection KW - Virulence -- genetics KW - RNA, Viral -- chemistry KW - Molecular Sequence Data KW - Macaca mulatta KW - RNA, Viral -- genetics KW - Binding Sites -- genetics KW - DNA, Viral -- genetics KW - Cell Line KW - Hepatitis E virus -- pathogenicity KW - Hepatitis E virus -- genetics KW - Hepatitis E virus -- physiology KW - Genes, Viral UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67823175?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=The+open+reading+frame+3+gene+of+hepatitis+E+virus+contains+a+cis-reactive+element+and+encodes+a+protein+required+for+infection+of+macaques.&rft.au=Graff%2C+Judith%3BNguyen%2C+Hanh%3BYu%2C+Claro%3BElkins%2C+William+R%3BSt+Claire%2C+Marisa%3BPurcell%2C+Robert+H%3BEmerson%2C+Suzanne+U&rft.aulast=Graff&rft.aufirst=Judith&rft.date=2005-06-01&rft.volume=79&rft.issue=11&rft.spage=6680&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-14 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Virol. 1999 May;73(5):4074-82 [10196303] Protein Expr Purif. 1998 Feb;12(1):75-84 [9473460] J Virol. 2005 Jan;79(2):1017-26 [15613330] Lancet. 2003 Aug 2;362(9381):371-3 [12907011] J Gen Virol. 2003 Sep;84(Pt 9):2351-7 [12917455] J Virol. 2004 Jan;78(1):320-8 [14671114] J Virol. 2004 May;78(9):4838-46 [15078965] J Biol Chem. 2004 Jul 2;279(27):28345-57 [15096509] J Biol Chem. 2004 Jul 9;279(28):29308-19 [15037615] Cancer Res. 1982 Sep;42(9):3858-63 [6286115] Virology. 1991 Nov;185(1):120-31 [1926770] J Virol. 1996 Jan;70(1):207-16 [8523527] Clin Diagn Lab Immunol. 1997 Jul;4(4):423-8 [9220158] J Virol. 2000 Jun;74(12):5548-55 [10823861] J Hepatol. 2000 Nov;33(5):842-5 [11097496] Virology. 2001 Jul 5;285(2):322-31 [11437666] J Biol Chem. 2001 Nov 9;276(45):42389-400 [11518702] Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15270-5 [11742081] J Biol Chem. 2002 Jun 21;277(25):22759-67 [11934888] Wei Sheng Wu Xue Bao. 2000 Dec;40(6):622-7 [12549057] J Virol. 1997 Dec;71(12):9045-53 [9371561] J Gen Virol. 1999 May;80 ( Pt 5):1185-8 [10355765] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Activation of the canonical Wnt/beta-catenin pathway confers growth advantages in c-Myc/E2F1 transgenic mouse model of liver cancer. AN - 67816970; 15885355 AB - Previously, we showed that activation of the beta-catenin/Wnt pathway is a dominant event during c-Myc/E2F1 hepatocarcinogenesis. Majority of c-Myc/E2F1 HCCs displayed nuclear accumulation of beta-catenin in the absence of beta-catenin mutations, suggesting that alterations in other members of the Wnt pathway might be responsible for nuclear localization of beta-catenin. Here, we investigated the mechanisms responsible for nuclear translocation of wild-type beta-catenin and addressed the potential contribution of the Wnt pathway in c-Myc/E2F1 hepatocarcinogenesis. Status of the members of the Wnt pathway was determined through microsatellite and Western blot analysis. Majority of c-Myc/E2F1 HCCs exhibited multiple abnormalities in the Wnt pathway regardless of the presence of beta-catenin mutations. The observed abnormalities included overexpression of Wnt-1, Frizzled 1 and 2 receptors, Dishevelled-1, downregulation of Secreted frizzled-related protein-1, GSK-3beta inactivation, microsatellite instability at the Axin locus as well as induction of beta-catenin target genes, such as glutamine synthetase, glutamate transporter-1, and Wisp-1. HCCs with beta-catenin activation displayed significantly higher proliferation rate and larger tumor size when compared with beta-catenin negative tumors. The data demonstrate that multiple abnormalities in the members of the Wnt pathway lead to nuclear accumulation of beta-catenin and suggest that activation of Wnt pathway provides proliferative advantages in c-Myc/E2F1-driven hepatocarcinogenesis. JF - Journal of hepatology AU - Calvisi, Diego F AU - Conner, Elizabeth A AU - Ladu, Sara AU - Lemmer, Eric R AU - Factor, Valentina M AU - Thorgeirsson, Snorri S AD - Laboratory of Experimental Carcinogenesis, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Building 37, Room 4146A, 37 Convent Drive MSC 4262, Bethesda, MD 20892-4262, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 842 EP - 849 VL - 42 IS - 6 SN - 0168-8278, 0168-8278 KW - CTNNB1 protein, mouse KW - 0 KW - Cell Cycle Proteins KW - Cytoskeletal Proteins KW - DNA-Binding Proteins KW - E2F Transcription Factors KW - E2F1 Transcription Factor KW - E2f1 protein, mouse KW - Intercellular Signaling Peptides and Proteins KW - Trans-Activators KW - Transcription Factors KW - Wnt Proteins KW - Wnt1 Protein KW - Wnt1 protein, mouse KW - beta Catenin KW - Glycogen Synthase Kinase 3 beta KW - EC 2.7.11.1 KW - Gsk3b protein, mouse KW - Glycogen Synthase Kinase 3 KW - EC 2.7.11.26 KW - Index Medicus KW - Animals KW - Cell Division -- physiology KW - Disease Models, Animal KW - Mice KW - Precancerous Conditions -- metabolism KW - Mice, Transgenic KW - Precancerous Conditions -- pathology KW - Microsatellite Repeats KW - Gene Expression Profiling KW - Precancerous Conditions -- genetics KW - Glycogen Synthase Kinase 3 -- metabolism KW - Female KW - Male KW - Trans-Activators -- metabolism KW - Liver Neoplasms, Experimental -- genetics KW - Liver Neoplasms, Experimental -- metabolism KW - DNA-Binding Proteins -- genetics KW - Transcription Factors -- genetics KW - Intercellular Signaling Peptides and Proteins -- genetics KW - Signal Transduction -- physiology KW - Cell Cycle Proteins -- genetics KW - Liver Neoplasms, Experimental -- pathology KW - Trans-Activators -- genetics KW - Cytoskeletal Proteins -- metabolism KW - Genes, myc -- genetics KW - Intercellular Signaling Peptides and Proteins -- metabolism KW - Cytoskeletal Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67816970?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+hepatology&rft.atitle=Activation+of+the+canonical+Wnt%2Fbeta-catenin+pathway+confers+growth+advantages+in+c-Myc%2FE2F1+transgenic+mouse+model+of+liver+cancer.&rft.au=Calvisi%2C+Diego+F%3BConner%2C+Elizabeth+A%3BLadu%2C+Sara%3BLemmer%2C+Eric+R%3BFactor%2C+Valentina+M%3BThorgeirsson%2C+Snorri+S&rft.aulast=Calvisi&rft.aufirst=Diego&rft.date=2005-06-01&rft.volume=42&rft.issue=6&rft.spage=842&rft.isbn=&rft.btitle=&rft.title=Journal+of+hepatology&rft.issn=01688278&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-09 N1 - Date created - 2005-05-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A phase II and pharmacologic study of fluorouracil given by a 1-hour infusion daily for 5 days with leucovorin and interferon alpha-2a in adenocarcinoma of the large bowel. AN - 67802100; 15870935 AB - We have reported that increasing the length of infusion from 5 min to 1 h appeared to substantially reduce the toxicity associated with fluorouracil (5-FU) modulated by leucovorin (LV) and interferon alpha-2a (IFN-alpha). This phase II study assessed the antitumor efficacy of this regimen. Patients (n=38) with colorectal cancer received IFN-alpha 5 MU/m(2) SC on days 1-6; on days 2-6, LV 200 mg/m(2) IV was given with 5-FU at initial doses of 370-425 mg/m(2)/h. The regimen was well-tolerated with no grade 4 toxicity. At 425 mg/m(2) 5-FU, grade 3 toxicities included diarrhea (8.6%), anorexia, fever and fatigue (5.7% each), neutropenia and nausea/vomiting (2.9% each). Individuals tolerated 5-FU doses up to 644 mg/m(2). Objective responses occurred in 27% of 37 patients; median time to progression and survival were 6.1 and 12.9 months. Only 1 of 25 informative tumor samples had high-frequency microsatellite instability (MSI), while 7 of 23 assessable patients (30%) with MSI-negative tumors had an objective response. With 425 mg/m(2), the average 5-FU Cp and AUC(0-1 h) were 37.4 microM and 1161 microM/h. Some 6 patients had extended sampling, and the half-lives of 5-FU and FBAL (apparent) were 8.6 and 100.0 min, respectively. A 1-h infusion of 5-FU is well tolerated; individual dose escalation of 5-FU allows each patient to receive the maximum tolerable dose. JF - Oncology reports AU - Ismail, Abdel Salam Attia AU - Quinn, Mary G AU - Wright, Maurice A AU - Ernst, Aaron AU - Kao, Vivian AU - Grogan, Liam AU - Parr, Allison AU - Grollman, Frank AU - Kirsch, Ilan R AU - Grem, Jean L AD - Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute-Navy, National Naval Medical Center, Bethesda, MD 20889-5105, USA. Y1 - 2005/06// PY - 2005 DA - June 2005 SP - 1145 EP - 1152 VL - 13 IS - 6 SN - 1021-335X, 1021-335X KW - Interferon-alpha KW - 0 KW - Recombinant Proteins KW - interferon alfa-2a KW - 47RRR83SK7 KW - Thymidylate Synthase KW - EC 2.1.1.45 KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Drug Administration Schedule KW - Area Under Curve KW - Interferon-alpha -- administration & dosage KW - Humans KW - Intestine, Large -- metabolism KW - Aged KW - Fluorouracil -- administration & dosage KW - Survival Rate KW - Adult KW - Treatment Outcome KW - Middle Aged KW - Maximum Tolerated Dose KW - Thymidylate Synthase -- metabolism KW - Female KW - Male KW - Intestine, Large -- pathology KW - Adenocarcinoma -- metabolism KW - Colorectal Neoplasms -- metabolism KW - Antineoplastic Combined Chemotherapy Protocols -- pharmacokinetics KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Adenocarcinoma -- drug therapy KW - Colorectal Neoplasms -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67802100?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncology+reports&rft.atitle=A+phase+II+and+pharmacologic+study+of+fluorouracil+given+by+a+1-hour+infusion+daily+for+5+days+with+leucovorin+and+interferon+alpha-2a+in+adenocarcinoma+of+the+large+bowel.&rft.au=Ismail%2C+Abdel+Salam+Attia%3BQuinn%2C+Mary+G%3BWright%2C+Maurice+A%3BErnst%2C+Aaron%3BKao%2C+Vivian%3BGrogan%2C+Liam%3BParr%2C+Allison%3BGrollman%2C+Frank%3BKirsch%2C+Ilan+R%3BGrem%2C+Jean+L&rft.aulast=Ismail&rft.aufirst=Abdel+Salam&rft.date=2005-06-01&rft.volume=13&rft.issue=6&rft.spage=1145&rft.isbn=&rft.btitle=&rft.title=Oncology+reports&rft.issn=1021335X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Biotechnology Products and University-Based Science AN - 21281488; 6427628 JF - JAMA: Journal of the American Medical Association AU - Sobolski, Gregory K AD - Department of Clinical Bioethics , National Institutes of Health , Bethesda, Md, sobolskig@cc.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2862 PB - American Medical Association, 515 N. State St. Chicago IL 60610 USA VL - 293 IS - 23 SN - 0098-7484, 0098-7484 KW - Biotechnology and Bioengineering Abstracts KW - Biotechnology KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21281488?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=JAMA%3A+Journal+of+the+American+Medical+Association&rft.atitle=Biotechnology+Products+and+University-Based+Science&rft.au=Sobolski%2C+Gregory+K&rft.aulast=Sobolski&rft.aufirst=Gregory&rft.date=2005-06-01&rft.volume=293&rft.issue=23&rft.spage=2862&rft.isbn=&rft.btitle=&rft.title=JAMA%3A+Journal+of+the+American+Medical+Association&rft.issn=00987484&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-03-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Biotechnology ER - TY - JOUR T1 - A Phase I Pharmacologic and Pharmacogenetic Trial of Sequential 24-Hour Infusion of Irinotecan Followed by Leucovorin and a 48-Hour Infusion of Fluorouracil in Adult Patients with Solid Tumors AN - 21267648; 6417006 AB - PURPOSE: In preclinical studies, sequential exposure to irinotecan (CPT-11) then fluorouracil (5-FU) is superior to concurrent exposure or the reverse sequence; a 24-hour infusion of CPT-11 may be better tolerated than shorter infusions. Experimental Design: CPT-11 was first given at four levels (70-140 mg/m super(2)/24 hours), followed by leucovorin 500 mg/m super(2)/0.5 hours and 5-FU 2,000 mg/m super(2)/48 hours on days 1 and 15 of a 4-week cycle. 5-FU was then increased in three cohorts up to 3,900 mg/m super(2)/48 hours. RESULTS: Two patients had dose-limiting toxicity during cycle 1 at 140/3,900 of CPT-11/5-FU (2-week delay for neutrophil recovery; grade 3 nausea despite antiemetics); one of six patients at 140/3,120 had dose-limiting toxicity (grade 3 diarrhea, grade 4 neutropenia). Four of 22 patients with colorectal cancer had partial responses, two of which had prior bolus CPT-11/5-FU. The mean 5-FU plasma concentration was 5.1 kmol/L at 3,900 mg/m super(2)/48 hours. The end of infusion CPT-11 plasma concentration averaged 519 nmol/L at 140 mg/m super(2)/24 hours. Patients with UDP-glucuronosyltransferase (UGT1A1; TA)6/6 promoter genotype had a lower ratio of free to glucuronide form of SN-38 than in patients with .1 (TA)7 allele. Thymidylate synthase genotypes for the 28-base promoter repeat were 2/2 (13%), 2/3 (74%), 3/3 (13%); all four responders had a 2/3 genotype. CONCLUSIONS: Doses (mg/m super(2)) of CPT-11 140/24 hours, leucovorin 500/0.5 hours and 5-FU 3,120/48 hours were well tolerated. JF - Clinical Cancer Research AU - Wright, Maurice A AU - Morrison, Geraldine AU - Lin, Pengxin AU - Leonard, Gregory D AU - Nguyen, Dat AU - Guo, Xaiodu AU - Szabo, Eva AU - Hopkins, Jon L AU - Leguizamo, Jorge P AU - Harold, Nancy AU - Fioravanti, Suzanne AU - Schuler, Barbara AU - Monahan, Brian P AU - Saif, MWasif AU - Quinn, Mary G AU - Pang, Janet AU - Grem, Jean L AD - Authors' Affiliations: Gastrointestinal Tumor Section, Cancer Therapeutics Branch, and Medical Oncology Clinical Research Unit, Center for Cancer Research, Cancer Prevention Program, National Cancer Institute, Division of Hematology and Medical Oncology, National Naval Medical Center, Bethesda, Maryland Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 4144 EP - 4150 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 11 SN - 1078-0432, 1078-0432 KW - Toxicology Abstracts KW - Diarrhea KW - Solid tumors KW - Irinotecan KW - UDP-glucuronosyltransferase KW - Leukocytes (neutrophilic) KW - Colorectal cancer KW - Antiemetics KW - Toxicity KW - Genotypes KW - Clinical trials KW - Pharmacogenetics KW - Thymidylate synthase KW - Neutropenia KW - Promoters KW - Nausea KW - X 24310:Pharmaceuticals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21267648?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=A+Phase+I+Pharmacologic+and+Pharmacogenetic+Trial+of+Sequential+24-Hour+Infusion+of+Irinotecan+Followed+by+Leucovorin+and+a+48-Hour+Infusion+of+Fluorouracil+in+Adult+Patients+with+Solid+Tumors&rft.au=Wright%2C+Maurice+A%3BMorrison%2C+Geraldine%3BLin%2C+Pengxin%3BLeonard%2C+Gregory+D%3BNguyen%2C+Dat%3BGuo%2C+Xaiodu%3BSzabo%2C+Eva%3BHopkins%2C+Jon+L%3BLeguizamo%2C+Jorge+P%3BHarold%2C+Nancy%3BFioravanti%2C+Suzanne%3BSchuler%2C+Barbara%3BMonahan%2C+Brian+P%3BSaif%2C+MWasif%3BQuinn%2C+Mary+G%3BPang%2C+Janet%3BGrem%2C+Jean+L&rft.aulast=Wright&rft.aufirst=Maurice&rft.date=2005-06-01&rft.volume=11&rft.issue=11&rft.spage=4144&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-02-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Diarrhea; Solid tumors; Irinotecan; Colorectal cancer; Leukocytes (neutrophilic); UDP-glucuronosyltransferase; Antiemetics; Genotypes; Toxicity; Clinical trials; Pharmacogenetics; Thymidylate synthase; Promoters; Neutropenia; Nausea ER - TY - JOUR T1 - Phase I Study of the Farnesyltransferase Inhibitor BMS-214662 Given Weekly in Patients with Solid Tumors AN - 21267232; 6417007 AB - PURPOSE: A phase I trial of BMS-214662, a selective farnesyltransferase inhibitor with significant preclinical antitumor activity in which drug was given as a weekly 1-hour infusion for four of six weeks, was conducted to evaluate the tolerability, pharmacokinetics, and pharmacodynamic effect on farnesyltransferase activity in peripheral blood mononuclear cells. Experimental Design: BMS-214662 was given to 27 patients with solid tumors at 10 escalating dose levels (28-220 mg/m super(2)) allowing intrapatient dose escalation; pharmacokinetics and pharmacodynamics were done at the first seven dose levels. RESULTS: Grade 4 neutropenia (four patients) was the most common dose-limiting toxicity followed by aminotransferase elevation (grade 3 alanine aminotransferase and grade 4 aspartate aminotransferase) and grade 3 dehydration. Most frequent toxicities were neutropenia in 11 (14%), anemia in 15 (19%), fatigue in 9 (12%), and nausea and diarrhea in 6 (8%) of courses, respectively. One minor response lasting 18 weeks in a patient with non-small cell lung cancer, serum calcitonin level reduction accompanied by disease stabilization in two of four patients with medullary thyroid carcinoma, and stable disease in 16 of 25 evaluable patients was seen. No correlation was observed between dose and C sub(max), total body clearance (mean, 26.15 c 10.88 L per hour per m super(2)), volume of distribution at steady state (mean, 39.51 c 17.91 L/m super(2)), or half-life (mean, 2.63 c 1.81 hours); a moderate correlation existed between dose given and systemic drug exposure (AUC). Substantial inhibition of peripheral blood mononuclear cell farnesyltransferase activity but near complete recovery by 24 hours was seen. CONCLUSION: BMS-214667 was well tolerated as a weekly 1-hour i.v. infusion for four of six weeks with evidence of pharmacodynamic effect. The study was terminated before maximum tolerated dose was reached. Alternative schedules of drug administration might result in improved pharmacodynamic profile. JF - Clinical Cancer Research AU - Papadimitrakopoulou, Vali AU - Agelaki, Sofia AU - Tran, Hai T AU - Kies, Merrill AU - Gagel, Robert AU - Zinner, Ralph AU - Kim, Edward AU - Ayers, Gregory AU - Wright, John AU - Khuri, Fadlo AD - Authors' Affiliations: The University of Texas M.D. Anderson Cancer Center, Houston, Texas, National Cancer Institute Cancer Therapy Evaluation Program, Bethesda, Maryland, and Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 4151 EP - 4159 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 11 SN - 1078-0432, 1078-0432 KW - Toxicology Abstracts KW - Calcitonin KW - Diarrhea KW - Fatigue KW - Aspartate aminotransferase KW - Solid tumors KW - thyroid carcinoma KW - Non-small cell lung carcinoma KW - Anemia KW - Toxicity KW - Alanine transaminase KW - Clinical trials KW - Pharmacokinetics KW - Neutropenia KW - Peripheral blood mononuclear cells KW - Farnesyl-diphosphate farnesyltransferase KW - Nausea KW - Drugs KW - Pharmacodynamics KW - Antitumor activity KW - Dehydration KW - X 24310:Pharmaceuticals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21267232?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Phase+I+Study+of+the+Farnesyltransferase+Inhibitor+BMS-214662+Given+Weekly+in+Patients+with+Solid+Tumors&rft.au=Papadimitrakopoulou%2C+Vali%3BAgelaki%2C+Sofia%3BTran%2C+Hai+T%3BKies%2C+Merrill%3BGagel%2C+Robert%3BZinner%2C+Ralph%3BKim%2C+Edward%3BAyers%2C+Gregory%3BWright%2C+John%3BKhuri%2C+Fadlo&rft.aulast=Papadimitrakopoulou&rft.aufirst=Vali&rft.date=2005-06-01&rft.volume=11&rft.issue=11&rft.spage=4151&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-02-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Calcitonin; Fatigue; Diarrhea; Solid tumors; Aspartate aminotransferase; Non-small cell lung carcinoma; thyroid carcinoma; Anemia; Toxicity; Alanine transaminase; Clinical trials; Pharmacokinetics; Neutropenia; Peripheral blood mononuclear cells; Farnesyl-diphosphate farnesyltransferase; Nausea; Drugs; Pharmacodynamics; Dehydration; Antitumor activity ER - TY - JOUR T1 - Genomics update: The vibrio that sheds light AN - 21119222; 6255244 JF - Environmental Microbiology AU - Galperin, Michael Y AD - 1 National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA, galperin@ncbi.nlm.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 757 EP - 760 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 7 IS - 6 SN - 1462-2912, 1462-2912 KW - Microbiology Abstracts B: Bacteriology KW - Vibrio KW - genomics KW - J 02310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21119222?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Microbiology&rft.atitle=Genomics+update%3A+The+vibrio+that+sheds+light&rft.au=Galperin%2C+Michael+Y&rft.aulast=Galperin&rft.aufirst=Michael&rft.date=2005-06-01&rft.volume=7&rft.issue=6&rft.spage=757&rft.isbn=&rft.btitle=&rft.title=Environmental+Microbiology&rft.issn=14622912&rft_id=info:doi/10.1111%2Fj.1462-2920.2005.00839.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-01-01 N1 - SuppNotes - Tables, 1; references, 13. N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - genomics; Vibrio DO - http://dx.doi.org/10.1111/j.1462-2920.2005.00839.x ER - TY - JOUR T1 - Comparative metabolism of methacrylonitrile and acrylonitrile to cyanide using cytochrome P4502E1 and microsomal epoxide hydrolase-null mice AN - 20777374; 8250360 AB - Methacrylonitrile (MAN) and acrylonitrile (AN) are metabolized via glutathione (GSH) conjugation or epoxide formation. We have recently shown that CYP2E1 is essential for AN epoxidation and subsequent cyanide liberation. Current studies were designed to compare the enzymatic basis of MAN vs. AN metabolism to cyanide using wild-type (WT), CYP2E1-, and mEH-null mice. Mice received a single gavage dose of 0.047, 0.095, 0.19, or 0.38 mmol/kg of MAN or AN, and blood cyanide was measured at 1 or 3 h later. Blood cyanide levels in WT mice treated with AN or MAN were dose and time dependent. At equimolar doses, significantly higher levels of cyanide were detected in the blood of MAN- vs. AN-treated mice. Further, while significant reduction in blood cyanide levels occurred in MAN-treated CYP2E1-null vs. WT mice, AN metabolism to cyanide was largely abolished in CYP2E1-null mice. Pretreatment of mice with 1-aminobenzotriazole (ABT, CYP inhibitor) demonstrated that CYPs other than CYP2E1 also contribute to MAN metabolism to cyanide. Blood cyanide levels in mEH-null mice treated with aliphatic nitriles are generally lower than levels in similarly treated WT mice. Western blot analysis showed that expression of sEH was greater in male vs. female mice. The role of various epoxide hydrolases (EHs) in the production of cyanide from aliphatic nitriles is apparently structure and dose dependent. Regardless of genotype, significantly higher levels of cyanide were measured in the blood of male vs. female mice treated with MAN or AN. In conclusion, these data showed that (1) at equimolar doses, higher blood cyanide levels were detected in mice treated with MAN vs. AN; (2) while CYP2E1 is the only enzyme responsible for AN metabolism to cyanide, other CYPs also contribute to MAN metabolism; and (3) significantly higher levels of cyanide were measured in the blood of male vs. female treated with either nitrile. Higher blood cyanide levels in male vs. female mice and in MAN- vs. AN-treated mice may explain the gender-related differences in the toxicity of these chemicals and the greater potency of MAN vs. AN. JF - Toxicology and Applied Pharmacology AU - El Hadri, L AU - Chanas, B AU - Ghanayem, B I AD - National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD (B3-10), Research Triangle Park, NC 27709, USA, ghanayem@niehs.nih.gov Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 116 EP - 125 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 205 IS - 2 SN - 0041-008X, 0041-008X KW - Toxicology Abstracts KW - Western blotting KW - Epoxides KW - Glutathione KW - Enzymes KW - Staphylococcal enterotoxin H KW - Genotypes KW - Toxicity KW - Sex differences KW - Acrylonitrile KW - Blood KW - Cyanide KW - Epoxidation KW - Epoxide hydrolase KW - Metabolism KW - X 24330:Agrochemicals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20777374?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Comparative+metabolism+of+methacrylonitrile+and+acrylonitrile+to+cyanide+using+cytochrome+P4502E1+and+microsomal+epoxide+hydrolase-null+mice&rft.au=El+Hadri%2C+L%3BChanas%2C+B%3BGhanayem%2C+B+I&rft.aulast=El+Hadri&rft.aufirst=L&rft.date=2005-06-01&rft.volume=205&rft.issue=2&rft.spage=116&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.10.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Western blotting; Epoxides; Glutathione; Enzymes; Staphylococcal enterotoxin H; Toxicity; Genotypes; Sex differences; Acrylonitrile; Blood; Cyanide; Epoxidation; Epoxide hydrolase; Metabolism DO - http://dx.doi.org/10.1016/j.taap.2004.10.002 ER - TY - JOUR T1 - Paclitaxel chemotherapy: from empiricism to a mechanism-based formulation strategy AN - 20696018; 10290487 AB - Paclitaxel is an anticancer agent effective for the treatment of breast, ovarian, lung, and head and neck cancer. Because of water insolubility, paclitaxel is formulated with the micelle-forming vehicle Cremophor EL to enhance drug solubility. However, the addition of Cremophor EL results in hypersensitivity reactions, neurotoxicity, and altered pharmacokinetics of paclitaxel. To circumvent these unfavorable effects resulting from the addition of Cremophor EL, efforts have been made to develop new delivery systems for paclitaxel administration. For example, ABI-007 is a Cremophor-free, albumin-stabilized, nanoparticle paclitaxel formulation that was found to have significantly less toxicity than Cremophor-containing paclitaxel in mice. Pharmacokinetic studies indicate that in contrast to Cremophor-containing paclitaxel, ABI-007 displays linear pharmacokinetics over the clinically relevant dose range of 135a300 mg/m super(2). In a phase III study conducted in patients with metastatic breast cancer, patients treated with ABI-007 achieved a significantly higher objective response rate and time to progression than those treated with Cremophor-containing paclitaxel. Together these findings suggest that nanoparticle albumin-bound paclitaxel may enable clinicians to administer paclitaxel at higher doses with less toxicity than is seen with Cremophor-containing paclitaxel. The role of this novel paclitaxel formulation in combination therapy with other antineoplastic agents needs to be determined. JF - Therapeutics and Clinical Risk Management AU - Scripture, Charity D AU - Figg, William D AU - Sparreboom, Alex AD - Clinical Pharmacology Research Core, National Cancer Institute Bethesda, MD, USA Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 107 EP - 114 PB - Dove Medical Press Ltd, Beechfield House Macclesfield SK11 0JL UK VL - 1 IS - 2 SN - 1176-6336, 1176-6336 KW - Risk Abstracts KW - Mice KW - Toxicity KW - Pharmacokinetics KW - Cancer KW - chemotherapy KW - hypersensitivity KW - Lung KW - Neurotoxicity KW - Breast cancer KW - Drugs KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20696018?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Therapeutics+and+Clinical+Risk+Management&rft.atitle=Paclitaxel+chemotherapy%3A+from+empiricism+to+a+mechanism-based+formulation+strategy&rft.au=Scripture%2C+Charity+D%3BFigg%2C+William+D%3BSparreboom%2C+Alex&rft.aulast=Scripture&rft.aufirst=Charity&rft.date=2005-06-01&rft.volume=1&rft.issue=2&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Therapeutics+and+Clinical+Risk+Management&rft.issn=11766336&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-08-01 N1 - Last updated - 2011-12-15 N1 - SubjectsTermNotLitGenreText - hypersensitivity; Lung; Neurotoxicity; Breast cancer; Mice; Toxicity; Drugs; chemotherapy; Cancer; Pharmacokinetics ER - TY - JOUR T1 - Alignments anchored on genomic landmarks can aid in the identification of regulatory elements AN - 20483362; 7873129 AB - Motivation: The transcription start site (TSS) has been located for an increasing number of genes across several organisms. Statistical tests have shown that some cis-acting regulatory elements have positional preferences with respect to the TSS, but few strategies have emerged for locating elements by their positional preferences. This paper elaborates such a strategy. First, we align promoter regions without gaps, anchoring the alignment on each promoter's TSS. Second, we apply a novel word-specific mask. Third, we apply a clustering test related to gapless BLAST statistics. The test examines whether any specific word is placed unusually consistently with respect to the TSS. Finally, our program A-GLAM, an extension of the GLAM program, uses significant word positions as new 'anchors' to realign the sequences. A Gibbs sampling algorithm then locates putative cis-acting regulatory elements. Usually, Gibbs sampling requires a preliminary masking step, to avoid convergence onto a dominant but uninteresting signal from a DNA repeat. However, since the positional anchors focus A-GLAM on the motif of interest, masking DNA repeats during Gibbs sampling becomes unnecessary. Results: In a set of human DNA sequences with experimentally characterized TSSs, the placement of 791 octonucleotide words was unusually consistent (multiple test corrected P < 0.05). Alignments anchored on these words sometimes located statistically significant motifs inaccessible to GLAM or AlignACE. Availability: The A-GLAM program and a list of statistically significant words are available at ftp://ftp.ncbi.nih.gov/pub/spouge/papers/archive/AGLAM/. JF - Bioinformatics AU - Tharakaraman, Kannan AU - MariFo-Ramirez, Leonardo AU - Sheetlin, Sergey AU - Landsman, David AU - Spouge, John L Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1 PB - Oxford University Press, Oxford Journals, Great Clarendon Street VL - 21 IS - S1 SN - 1367-4803, 1367-4803 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Statistics KW - Regulatory sequences KW - Nucleotide sequence KW - Statistical analysis KW - Algorithms KW - Transcription KW - Promoters KW - Convergence KW - Sampling KW - Bioinformatics KW - genomics KW - Repeated DNA sequences KW - G 07880:Human Genetics KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20483362?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Alignments+anchored+on+genomic+landmarks+can+aid+in+the+identification+of+regulatory+elements&rft.au=Tharakaraman%2C+Kannan%3BMariFo-Ramirez%2C+Leonardo%3BSheetlin%2C+Sergey%3BLandsman%2C+David%3BSpouge%2C+John+L&rft.aulast=Tharakaraman&rft.aufirst=Kannan&rft.date=2005-06-01&rft.volume=21&rft.issue=S1&rft.spage=i440&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Promoters; Statistics; Convergence; Nucleotide sequence; Regulatory sequences; Algorithms; Statistical analysis; Transcription; genomics; Bioinformatics; Sampling; Repeated DNA sequences ER - TY - JOUR T1 - Predicting protein-protein interaction by searching evolutionary tree automorphism space AN - 20472366; 7873157 AB - Motivation: Uncovering the protein-protein interaction network is a fundamental step in the quest to understand the molecular machinery of a cell. This motivates the search for efficient computational methods for predicting such interactions. Among the available predictors are those that are based on the co-evolution hypothesis "evolutionary trees of protein families (that are known to interact) are expected to have similar topologies". Many of these methods are limited by the fact that they can handle only a small number of protein sequences. Also, details on evolutionary tree topology are missing as they use similarity matrices in lieu of the trees. Results: We introduce MORPH, a new algorithm for predicting protein interaction partners between members of two protein families that are known to interact. Our approach can also be seen as a new method for searching the best superposition of the corresponding evolutionary trees based on tree automorphism group. We discuss relevant facts related to the predictability of protein-protein interaction based on their co-evolution. When compared with related computational approaches, our method reduces the search space by similar to 3 x 10 super( 5 ) -fold and at the same time increases the accuracy of predicting correct binding partners JF - Bioinformatics AU - Jothi, Raja AU - Kann, Maricel G AU - Przytycka, Teresa M Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1 PB - Oxford University Press, Oxford Journals, Great Clarendon Street VL - 21 IS - S1 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts KW - Computer programs KW - Algorithms KW - protein families KW - Bioinformatics KW - Computer applications KW - Protein interaction KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20472366?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Predicting+protein-protein+interaction+by+searching+evolutionary+tree+automorphism+space&rft.au=Jothi%2C+Raja%3BKann%2C+Maricel+G%3BPrzytycka%2C+Teresa+M&rft.aulast=Jothi&rft.aufirst=Raja&rft.date=2005-06-01&rft.volume=21&rft.issue=S1&rft.spage=i241&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Computer programs; Algorithms; protein families; Bioinformatics; Computer applications; Protein interaction ER - TY - JOUR T1 - A Combined Yeast/Bacteria Two-hybrid System: Development and Evaluation AN - 20150068; 6417232 AB - Two-hybrid screening is a standard method used to identify and characterize protein-protein interactions and has become an integral component of many proteomic investigations. The two-hybrid system was initially developed using yeast as a host organism. However, bacterial two-hybrid systems have also become common laboratory tools and are preferred in some circumstances, although yeast and bacterial two-hybrid systems have never been directly compared. We describe here the development of a unified yeast and bacterial two-hybrid system in which a single bait expression plasmid is used in both organismal milieus. We use a series of leucine zipper fusion proteins of known affinities to compare interaction detection using both systems. Although both two-hybrid systems detected interactions within a comparable range of interaction affinities, each demonstrated unique advantages. The yeast system produced quantitative readout over a greater dynamic range than that observed with bacteria. However, the phenomenon of "autoactivation" by baits was less of a problem in the bacterial system than in the yeast. Both systems identified physiological interactors for a library screen with a cI-Ras test bait; however, non-identical interactors were obtained in yeast and bacterial screens. The ability to rapidly shift between yeast and bacterial systems provided by these new reagents should provide a marked advantage for two-hybrid investigations. In addition, the modified expression vectors we describe in this report should be useful for any application requiring facile expression of a protein of interest in both yeast and bacteria. JF - Molecular and Cellular Proteomics AU - Serebriiskii, Ilya G AU - Fang, Rui AU - Latypova, Ekaterina AU - Hopkins, Richard AU - Vinson, Charles AU - Joung, JKeith AU - Golemis, Erica A AD - Division of Basic Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, Molecular Pathology Unit, Department of Pathology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, Telethon Institute for Child Health Research, West Perth, WA 6872 Australia, Phylogica, Ltd., West Perth, WA 6872 Australia, and Division of Basic Science, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 819 EP - 826 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.asbmb.org/] VL - 4 IS - 6 SN - 1535-9476, 1535-9476 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Expression vectors KW - proteomics KW - Leucine zipper proteins KW - Development KW - Fusion protein KW - Plasmids KW - Protein interaction KW - J 02330:Biochemistry KW - K 03300:Methods KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20150068?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+Cellular+Proteomics&rft.atitle=A+Combined+Yeast%2FBacteria+Two-hybrid+System%3A+Development+and+Evaluation&rft.au=Serebriiskii%2C+Ilya+G%3BFang%2C+Rui%3BLatypova%2C+Ekaterina%3BHopkins%2C+Richard%3BVinson%2C+Charles%3BJoung%2C+JKeith%3BGolemis%2C+Erica+A&rft.aulast=Serebriiskii&rft.aufirst=Ilya&rft.date=2005-06-01&rft.volume=4&rft.issue=6&rft.spage=819&rft.isbn=&rft.btitle=&rft.title=Molecular+and+Cellular+Proteomics&rft.issn=15359476&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Expression vectors; Fusion protein; Development; Leucine zipper proteins; proteomics; Plasmids; Protein interaction ER - TY - JOUR T1 - The importance of being a pDC in antiviral immunity: the IFN mission versus Ag presentation? AN - 19891470; 8249815 AB - Plasmacytoid pre-dendritic cells (pDCs) comprise a pivotal element of antiviral immune responses. They recognize viral components, leading to type I interferon (IFN) production, and affect adaptive defense strategies designed to eliminate viral pathogens. These strategies include the ability of pDCs to modulate virus-specific CD8 super(+) T-cell responses. Although a great deal has been learned recently about pDCs, our knowledge of whether, how and why pDCs might function as antigen-presenting cells is extremely limited, and now is a prime time for exploring the unknowns of this area. This Opinion will focus on the IFN production and T-cell priming capacity of pDCs, and will argue that IFN production (and not T-cell priming) is the main function of pDCs during viral infection. JF - Trends in Immunology AU - Haeryfar, SMM Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 311 EP - 317 PB - Elsevier Science, The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 26 IS - 6 SN - 1471-4906, 1471-4906 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Virology & AIDS Abstracts; Immunology Abstracts KW - Interferon KW - Reviews KW - Lymphocytes T KW - Pathogens KW - Antigen-presenting cells KW - CD8 antigen KW - Immunity KW - Antigen presentation KW - Infection KW - A 01340:Antibiotics & Antimicrobials KW - V 22350:Immunology KW - F 06910:Microorganisms & Parasites UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19891470?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologya&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Trends+in+Immunology&rft.atitle=The+importance+of+being+a+pDC+in+antiviral+immunity%3A+the+IFN+mission+versus+Ag+presentation%3F&rft.au=Haeryfar%2C+SMM&rft.aulast=Haeryfar&rft.aufirst=SMM&rft.date=2005-06-01&rft.volume=26&rft.issue=6&rft.spage=311&rft.isbn=&rft.btitle=&rft.title=Trends+in+Immunology&rft.issn=14714906&rft_id=info:doi/10.1016%2Fj.it.2005.04.002 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Interferon; Reviews; Lymphocytes T; Immunity; CD8 antigen; Antigen-presenting cells; Pathogens; Infection; Antigen presentation DO - http://dx.doi.org/10.1016/j.it.2005.04.002 ER - TY - JOUR T1 - Bridging the imaging gap: visualizing subcellular architecture with electron tomography AN - 19813494; 6450716 AB - Transmission electron microscopy is a powerful tool that is used to explore the internal structure of tissues, cells, organelles and macromolecular complexes. By integrating data from a series of images in which the orientation of the specimen is progressively varied relative to the incident electron beam it is also possible to extend electron microscopic imaging into the third dimension. This approach, commonly referred to as electron tomography, has been greatly aided in recent years by advances in technology for imaging specimens at cryogenic temperatures, as well as by substantial progress in procedures for automated data collection and image processing. The intense pace of developments in this field is inspired, in a large part, by the hope that the quality of the data will ultimately be good enough to allow interpretation of tomograms of cells, organelles, bacteria and viruses in terms of the three-dimensional spatial arrangements of the constituent molecules. JF - Current Opinion in Microbiology AU - Subramaniam, S AD - Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health (NIH) Bethesda, MD 20892, USA, ss1@nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 316 EP - 322 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 8 IS - 3 SN - 1369-5274, 1369-5274 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Temperature effects KW - Macromolecules KW - Data processing KW - Transmission electron microscopy KW - Reviews KW - Tomography KW - Image processing KW - Data collections KW - Organelles KW - imaging KW - Cryogenics KW - V 22300:Methods KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19813494?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Opinion+in+Microbiology&rft.atitle=Bridging+the+imaging+gap%3A+visualizing+subcellular+architecture+with+electron+tomography&rft.au=Subramaniam%2C+S&rft.aulast=Subramaniam&rft.aufirst=S&rft.date=2005-06-01&rft.volume=8&rft.issue=3&rft.spage=316&rft.isbn=&rft.btitle=&rft.title=Current+Opinion+in+Microbiology&rft.issn=13695274&rft_id=info:doi/10.1016%2Fj.mib.2005.04.012 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-09-01 N1 - SuppNotes - Ecology and Industrial Microbiology. N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Temperature effects; Macromolecules; Data processing; Reviews; Transmission electron microscopy; Image processing; Tomography; Data collections; Organelles; imaging; Cryogenics DO - http://dx.doi.org/10.1016/j.mib.2005.04.012 ER - TY - JOUR T1 - Neutrophil inhibition with L-selectin-directed MAb improves or worsens survival dependent on the route but not severity of infection in a rat sepsis model AN - 19776834; 6278508 AB - Both route and severity of infection may influence immunomodulator agents in sepsis. We studied the effect of each variable on HRL-3, an L-selectin-directed MAb that inhibits neutrophil function, in a rat sepsis model. Animals (n = 800) were randomized to be treated with either HRL-3 or placebo and to receive Escherichia coli either intravenously (IV) or intrabronchially (IB) in doses producing low or high mortality rates. Animals received antibiotics and were observed for 168 h. Route but not dose of E. coli altered the effects HRL-3 on mortality rate (mean hazards ratio plus or minus SE). With IV E. coli, compared with control, HRL-3 was beneficial and reduced the hazards ratio both early (0 to 6 h; -0.75 plus or minus 0.23) and late (6 to 168 h; -0.72 plus or minus 0.36) (P = 0.001 and 0.04, respectively, over all E. coli doses). In contrast, with IB E. coli HRL-3 reduced the hazards ratio early (-1.1 plus or minus 0.36) but worsened it late (0.87 plus or minus 0.23) (P = 0.002 for both effects over all E. coli doses) in patterns significantly different from IV E. coli (P < 0.0001). Compared with control, although HRL-3 did not alter lung neutrophil numbers or injury score at 6 or 168 h with IV E. coli (P = ns for all), it reduced both early and increased them late with IB E. coli (P less than or equal to 0.05 for all comparing 6 with 168 h). Thus immunomodulators inhibiting neutrophil function, although potentially beneficial with sepsis due to intravascular infection, may be harmful with extravascular infection regardless of severity. JF - Journal of Applied Physiology AU - Haley, Michael AU - Parent, Chantal AU - Cui, Xizhong AU - Kalil, Andre AU - Fitz, Yvonne AU - Correa-Araujo, Rosaly AU - Natanson, Charles AU - Danner, Robert L AU - Banks, Steven M AU - Eichacker, Peter Q AD - Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2155 EP - 2162 PB - American Physiological Society, 9650 Rockville Pike Bethesda MD 20814-3991 USA, [mailto:webmaster@the-aps.org], [URL:http://www.the-aps.org/] VL - 98 IS - 6 SN - 8750-7587, 8750-7587 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Cell survival KW - Mortality KW - Injuries KW - Monoclonal antibodies KW - Leukocytes (neutrophilic) KW - Animal models KW - Antibiotics KW - Infection KW - Immunomodulation KW - Sepsis KW - Lung KW - Escherichia coli KW - J 02350:Immunology KW - F 06910:Microorganisms & Parasites UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19776834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Applied+Physiology&rft.atitle=Neutrophil+inhibition+with+L-selectin-directed+MAb+improves+or+worsens+survival+dependent+on+the+route+but+not+severity+of+infection+in+a+rat+sepsis+model&rft.au=Haley%2C+Michael%3BParent%2C+Chantal%3BCui%2C+Xizhong%3BKalil%2C+Andre%3BFitz%2C+Yvonne%3BCorrea-Araujo%2C+Rosaly%3BNatanson%2C+Charles%3BDanner%2C+Robert+L%3BBanks%2C+Steven+M%3BEichacker%2C+Peter+Q&rft.aulast=Haley&rft.aufirst=Michael&rft.date=2005-06-01&rft.volume=98&rft.issue=6&rft.spage=2155&rft.isbn=&rft.btitle=&rft.title=Journal+of+Applied+Physiology&rft.issn=87507587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Cell survival; Mortality; Sepsis; Injuries; Lung; Monoclonal antibodies; Animal models; Leukocytes (neutrophilic); Antibiotics; Infection; Immunomodulation; Escherichia coli ER - TY - JOUR T1 - Co-refolding of two peptide fragments derived from Agrobacterium tumefaciens beta -glucosidase with catalytic activity AN - 19703382; 7493466 AB - Four sites of the non-homologous region (coding amino acid residues of 347, 421, 466 and 533) of a gene were randomly selected for splitting to investigate the function of beta -glucosidase from Agrobacterium tumefaciens in the co-refolding of peptides into the catalytically active enzyme. As a result of gene splitting, four N- and C-terminal domain peptides were obtained as insoluble inclusion bodies. No catalytic activity was observed when these fragments refolded individually. However, a considerable amount of activity was restored when the two fragments derived from N- and C- terminal peptides were co-refolded together. The deletion of amino acid residues in the non-homologous region resulted in a complete loss of enzyme activity, which suggests that truncation of amino acids in this region strongly affects the co-refolding ability of the enzyme to maintain activity. JF - FEBS Letters AU - Kim, Bong-Jo AU - Mangala, Selanere L AU - Hayashi, Kiyoshi AD - Enzyme Laboratory, National Food Research Institute, 2-1-12 Kannondai, Tsukuba 305-8642, Japan, bjkim@mail.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 3075 EP - 3080 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 579 IS - 14 SN - 0014-5793, 0014-5793 KW - Microbiology Abstracts B: Bacteriology KW - beta -Glucosidase KW - Agrobacterium tumefaciens KW - Co-refolding KW - Gene splitting KW - Deletion KW - Amino acids KW - Enzymes KW - Inclusion bodies KW - Splitting KW - J 02330:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19703382?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=Co-refolding+of+two+peptide+fragments+derived+from+Agrobacterium+tumefaciens+beta+-glucosidase+with+catalytic+activity&rft.au=Kim%2C+Bong-Jo%3BMangala%2C+Selanere+L%3BHayashi%2C+Kiyoshi&rft.aulast=Kim&rft.aufirst=Bong-Jo&rft.date=2005-06-01&rft.volume=579&rft.issue=14&rft.spage=3075&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/10.1016%2Fj.febslet.2005.04.065 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-08-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Deletion; Amino acids; Inclusion bodies; Enzymes; beta -Glucosidase; Splitting; Agrobacterium tumefaciens DO - http://dx.doi.org/10.1016/j.febslet.2005.04.065 ER - TY - JOUR T1 - A Novel Polypyrimidine Antitumor Agent FdUMP[10] Induces Thymineless Death with Topoisomerase I-DNA Complexes AN - 19421759; 6413831 AB - FdUMP[10], a 10mer of 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP), the thymidylate synthase inhibitory metabolite of 5-fluorouracil (FU), is most closely correlated with the DNA topoisomerase I (Top1) inhibitor camptothecin in the National Cancer Institute COMPARE analysis, but not with FU. FdUMP[10] exhibits more potent antiproliferative activity than FdUMP or 5-fluoro-2'-deoxyuridine (FdU) and is markedly more active than FU. Camptothecin-resistant P388/CPT45 cells lacking Top1 are cross-resistant to FdUMP[10] as well as to FdUMP, FdU, and the thymidylate synthase inhibitor raltitrexed (Tomudex). FdUMP[10] induces DNA single-strand breaks and cellular Top1-DNA complexes. Such complexes are also observed in response to FdUMP, FdU, raltitrexed, and FU. The FdUMP[10]-induced Top1-DNA complexes are not inhibited by the caspase inhibitor z-VAD-fmk and form independently of apoptotic DNA fragmentation, indicating that they do not correspond to apoptotic Top1-DNA complexes. In biochemical assay, Top1 is directly trapped at uracil and FdU misincorporation sites. We propose that FdUMP[10] damages DNA by trapping Top1 at uracil and FdU misincorporation sites resulting from thymidylate synthase inhibition and thymine depletion. JF - Cancer Research AU - Liao, Zhi-Yong AU - Sordet, Olivier AU - Zhang, Hong-Liang AU - Kohlhagen, Glenda AU - Antony, Smitha AU - Gmeiner, William H AU - Pommier, Yves AD - Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, NIH, Department of Health and Human Services, Bethesda, Maryland and Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, North Carolina Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 4844 EP - 4851 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 65 IS - 11 SN - 0008-5472, 0008-5472 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - N 14015:Artificial oligonucleotides & nucleic acid analogs KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19421759?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Research&rft.atitle=A+Novel+Polypyrimidine+Antitumor+Agent+FdUMP%5B10%5D+Induces+Thymineless+Death+with+Topoisomerase+I-DNA+Complexes&rft.au=Liao%2C+Zhi-Yong%3BSordet%2C+Olivier%3BZhang%2C+Hong-Liang%3BKohlhagen%2C+Glenda%3BAntony%2C+Smitha%3BGmeiner%2C+William+H%3BPommier%2C+Yves&rft.aulast=Liao&rft.aufirst=Zhi-Yong&rft.date=2005-06-01&rft.volume=65&rft.issue=11&rft.spage=4844&rft.isbn=&rft.btitle=&rft.title=Cancer+Research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Influence of Excess Adiposity on Exercise Fitness and Performance in Overweight Children and Adolescents AN - 19420787; 6415674 AB - OBJECTIVE: Relatively little is known about how excess body mass affects adolescents' capacity to perform sustained exercise. We hypothesized that most of the difficulty that severely overweight adolescents have with sustained exercise occurs because the metabolic costs of moving excess mass result in use of a high proportion of their total oxygen reserve. METHODS: We compared results from a maximal cycle ergometry fitness test in 129 severely overweight adolescents who had BMIs of 41.5 plus or minus 9.7 kg/m super(2) and ages of 14.5 plus or minus 1.8 years (range: 12.1-17.8 years) and 34 nonoverweight adolescents who had BMIs of 20.1 plus or minus 2.9 kg/m super(2) and ages of 14.5 plus or minus 1.5 years (range: 12.0-18.1 years). Oxygen uptake ([Formula: see text]O sub(2)) was compared at 3 times: during a 4-minute period of unloaded cycling (UL[Formula: see text]O sub(2)), at the lactate threshold estimated by gas exchange (LT[Formula: see text]O sub(2)), and at maximal exertion ([Formula: see text]O sub(2) max). Heart rate was obtained at rest and at [Formula: see text]O sub(2) max. Participants also completed a 12-minute walk/run performance test to obtain distance traveled (D12) and heart rate. RESULTS: Absolute LT[Formula: see text]O sub(2) and [Formula: see text]O sub(2) max and LT[Formula: see text]O sub(2) as a percentage of [Formula: see text]O sub(2) max were not different in overweight and nonoverweight adolescents during the cycle test. However, absolute UL[Formula: see text]O sub(2) was significantly greater in overweight adolescents: UL[Formula: see text]O sub(2) accounted for 35 plus or minus 8% of [Formula: see text]O sub(2) max (and 63 plus or minus 15% of LT[Formula: see text]O sub(2)) in overweight adolescents but only 20 plus or minus 5% of [Formula: see text]O sub(2) max (and 39 plus or minus 12% of LT[Formula: see text]O sub(2)) in nonoverweight adolescents. Resting heart rate before initiating the cycle test was significantly greater in overweight than nonoverweight adolescents (94 plus or minus 14 vs 82 plus or minus 15 beats per minute). However, maximal heart rate during the cycle test was significantly lower in overweight adolescents (186 plus or minus 13 vs 196 plus or minus 11 beats per minute). During the walk/run test, mean D12 was significantly shorter for overweight than for nonoverweight adolescents (1983 plus or minus 323 vs 1159 plus or minus 194 m). D12 was negatively related to BMI SDS (r = -0.81) and to UL[Formula: see text]O sub(2) (r = -0.98). DISCUSSION: Overweight and nonoverweight adolescents had similar absolute [Formula: see text]O sub(2) at the lactate threshold and at maximal exertion, suggesting that overweight adolescents are more limited by the increased cardiorespiratory effort required to move their larger body mass through space than by cardiorespiratory deconditioning. The higher percentage of oxygen consumed during submaximal exercise indicates that overweight adolescents are burdened by the metabolic cost of their excess mass. Their greater oxygen demand during an unloaded task predicted poorer performance during sustained exercise. Exercise prescriptions for overweight adolescents should account for the limited exercise tolerance imposed by excess body mass, focusing on activities that keep demands below lactate threshold so that exercise can be sustained. JF - Pediatrics AU - Norman, Anne-Caroline AU - Drinkard, Bart AU - McDuffie, Jennifer R AU - Ghorbani, Samareh AU - Yanoff, Lisa B AU - Yanovski, Jack A AD - Unit on Growth and Obesity, Developmental Endocrinology Branch, National Institute on Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland. Duke University School of Medicine, Durham, North Carolina. Rehabilitation Medicine Department, Clinical Center, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - e690 EP - e696 PB - American Academy of Pediatrics, 141 Northwest Point Blvd. Elk Grove Village IL 60007-1098 USA, [mailto:journals@aap.org], [URL:http://www.aap.org] VL - 115 IS - 6 SN - 0031-4005, 0031-4005 KW - Physical Education Index KW - Fitness KW - Gas exchange KW - Obesity KW - Age KW - Anaerobic threshold KW - Pediatrics KW - Body mass KW - Adolescence KW - Ergometry KW - Heart rate KW - Exercise KW - Children KW - Exertion KW - Bicycling KW - Rest KW - Cardiorespiratory KW - Deconditioning KW - Performance KW - Activities KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420787?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatrics&rft.atitle=Influence+of+Excess+Adiposity+on+Exercise+Fitness+and+Performance+in+Overweight+Children+and+Adolescents&rft.au=Norman%2C+Anne-Caroline%3BDrinkard%2C+Bart%3BMcDuffie%2C+Jennifer+R%3BGhorbani%2C+Samareh%3BYanoff%2C+Lisa+B%3BYanovski%2C+Jack+A&rft.aulast=Norman&rft.aufirst=Anne-Caroline&rft.date=2005-06-01&rft.volume=115&rft.issue=6&rft.spage=e690&rft.isbn=&rft.btitle=&rft.title=Pediatrics&rft.issn=00314005&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2007-05-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Gas exchange; Fitness; Obesity; Anaerobic threshold; Age; Pediatrics; Ergometry; Adolescence; Body mass; Heart rate; Exercise; Exertion; Children; Bicycling; Rest; Deconditioning; Cardiorespiratory; Performance; Activities ER - TY - JOUR T1 - In vivo detection of cortical GABA turnover from intravenously infused [1- super(13)C]D-glucose AN - 19416005; 6487000 AB - In this study [2- super(13)C] gamma -aminobutyric acid (GABA) was spectrally resolved in vivo and detected simultaneously with [4- super(13)C]glutamate (Glu) and [4- super(13)C]glutamine (Gln) in the proton spectra obtained from a localized 40 mu L voxel in rat neocortex with the use of an adiabatic super(1)H-observed, super(13)C-edited (POCE) spectroscopy method and an 89-mm-bore vertical 11.7 Tesla microimager. The time-resolved kinetics of super(13)C label incorporation from intravenously infused [1- super(13)C]glucose into [4- super(13)C]Glu, [4- super(13)C]Gln, and [2- super(13)C]GABA were measured after acute administration of gabaculine, a potent and specific inhibitor of GABA-transaminase. In contrast to previous observations of a rapid turnover of [2- super(13)C]GABA from [1- super(13)C]glucose in intact rat brain, the rate of super(13)C incorporation from [1- super(13)C]glucose into [2- super(13)C]GABA in the gabaculine-treated rats was found to be significantly reduced as a result of the blockade of the GABA shunt. JF - Magnetic Resonance in Medicine AU - Yang, Jehoon AU - Li, Charles Q AU - Shen, Jun AD - Molecular Imaging Branch, National Institute of Mental Health, Bldg. 10, Rm. 2D51A, 9000 Rockville Pike, Bethesda, MD 20892-1527, USA, shenj@intra.nimh.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1258 EP - 1267 PB - John Wiley & Sons, Ltd. VL - 53 IS - 6 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Protons KW - Adiabatic KW - Kinetics KW - Shunts KW - Brain KW - N.M.R. KW - ^g-Aminobutyric acid KW - Spectroscopy KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19416005?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=In+vivo+detection+of+cortical+GABA+turnover+from+intravenously+infused+%5B1-+super%2813%29C%5DD-glucose&rft.au=Yang%2C+Jehoon%3BLi%2C+Charles+Q%3BShen%2C+Jun&rft.aulast=Yang&rft.aufirst=Jehoon&rft.date=2005-06-01&rft.volume=53&rft.issue=6&rft.spage=1258&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20473 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - ^g-Aminobutyric acid; Shunts; N.M.R.; Kinetics; Spectroscopy; Protons; Brain; Adiabatic DO - http://dx.doi.org/10.1002/mrm.20473 ER - TY - JOUR T1 - CELL BIOLOGY - IMMUNOLOGY - PATHOLOGY: Dendrimer-enhanced MRI as a diagnostic and prognostic biomarker of sepsis-induced acute renal failure in aged mice AN - 19287707; 6253516 AB - Background. Acute renal failure (ARF) induced by sepsis has a high mortality. In an aged mouse model of sepsis-induced ARF we have previously shown that renal injury occurs before serum creatinine is elevated. Development of a noninvasive biomarker that could diagnose renal dysfunction early in sepsis and monitor the response to therapy would be very valuable. Methods. We performed magnetic resonance imaging (MRI) with gadolinium-based G4 dendrimer intravenous contrast in a fluid- and antibiotic-treated cecal ligation and puncture (CLP) sepsis model in aged mice. Imaging was also performed in a mouse volume depletion model and in models of ARF induced by ischemia-reperfusion (I-R) and cisplatin. Results. Twenty hours post-CLP, aged mice had a distinct pattern of renal injury using dendrimer-enhanced MRI. This pattern was different from renal injury induced by either cisplatin or I-R. Prerenal azotemia induced by volume depletion was distinguished from sepsis by dendrimer-enhanced MRI. Dendrimer-enhanced MRI detected renal dysfunction 6 hours post-CLP, a time when serum creatinine was still normal. Ethyl pyruvate reversed the renal dysfunction detected by dendrimer-enhanced MRI at 20 hours, but not at 6 hours post-CLP. The appearance of renal dysfunction on dendrimer-enhanced MRI at 6 hours post-CLP predicted the length of survival. Conclusion. Dendrimer-enhanced MRI is a novel biomarker that provides information for the early diagnosis, drug responsiveness, and prognosis of sepsis-induced ARF. JF - Kidney International AU - Dear, James W AU - Kobayashi, Hisataka AU - Jo, Sang-Kyung AU - Holly, Mikaela K AU - Hu, Xuzhen AU - Yuen, Peter ST AU - Brechbiel, Martin W AU - Star, Robert A AD - Robert A. Star, M.D., Renal Diagnostics and Therapeutics, NIDDK, 10 Center Drive, Building 10, Room 3N108, Bethesda, MD 20892-1268, Robert_Star@nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2159 EP - 2167 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 67 IS - 6 SN - 0085-2538, 0085-2538 KW - Microbiology Abstracts B: Bacteriology KW - Mortality KW - Intravenous administration KW - Injuries KW - Magnetic resonance imaging KW - Animal models KW - Prognosis KW - Survival KW - Renal failure KW - biomarkers KW - Sepsis KW - Pyruvic acid KW - Creatinine KW - Cisplatin KW - Renal function KW - Cecum KW - Drugs KW - J 02410:Animal Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19287707?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Kidney+International&rft.atitle=CELL+BIOLOGY+-+IMMUNOLOGY+-+PATHOLOGY%3A+Dendrimer-enhanced+MRI+as+a+diagnostic+and+prognostic+biomarker+of+sepsis-induced+acute+renal+failure+in+aged+mice&rft.au=Dear%2C+James+W%3BKobayashi%2C+Hisataka%3BJo%2C+Sang-Kyung%3BHolly%2C+Mikaela+K%3BHu%2C+Xuzhen%3BYuen%2C+Peter+ST%3BBrechbiel%2C+Martin+W%3BStar%2C+Robert+A&rft.aulast=Dear&rft.aufirst=James&rft.date=2005-06-01&rft.volume=67&rft.issue=6&rft.spage=2159&rft.isbn=&rft.btitle=&rft.title=Kidney+International&rft.issn=00852538&rft_id=info:doi/10.1111%2Fj.1523-1755.2005.00321.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - SuppNotes - Figures, 8; formulas, 10; references, 25. N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Mortality; Intravenous administration; Injuries; Magnetic resonance imaging; Prognosis; Animal models; Renal failure; Survival; biomarkers; Sepsis; Creatinine; Pyruvic acid; Renal function; Cisplatin; Cecum; Drugs DO - http://dx.doi.org/10.1111/j.1523-1755.2005.00321.x ER - TY - JOUR T1 - Controlled Trial of Pamidronate in Children With Types III and IV Osteogenesis Imperfecta Confirms Vertebral Gains but Not Short-Term Functional Improvement AN - 17871887; 6251694 AB - Bisphosphonates have been widely administered to children with OI based on observational trials. A randomized controlled trial of q3m intravenous pamidronate in children with types III and IV OI yielded positive vertebral changes in DXA and geometry after 1 year of treatment, but no further significant improvement during extended treatment. The treated group did not experience significantly decreased pain or long bone fractures or have increased motor function or muscle strength. Introduction: Bisphosphonates, antiresorptive drugs for osteoporosis, are widely administered to children with osteogenesis imperfecta (OI). Uncontrolled pamidronate trials in OI reported increased BMD, vertebral coronal area, and mobility, and decreased pain. We conducted a randomized controlled trial of pamidronate in children with types III and IV OI. Materials and Methods: This randomized trial included 18 children (4-13 years of age) with types III and IV OI. The first study year was controlled; 9 children received pamidronate (10 mg/m super(2)/day IV for 3 days every 3 months). Four children in each group also received recombinant growth hormone (rGH) injections (0.06 mg/kg/day for 6 days/week). Seven children in the treatment group received pamidronate for an additional 6-21 months. All patients had L sub(1)-L sub(4) DXA, spine QCT, spine radiographs, and musculoskeletal and functional testing. Results: In the controlled phase, treated patients experienced a significant increase in L sub(1)-L sub(4) DXA z score (p < 0.001) and increased L sub(1)-L sub(4) midvertebral height (p = 0.014) and total vertebral area (p = 0.003) compared with controls. During extended treatment, DXA z scores and vertebral heights and areas did not increase significnatly beyond the 12-month values. Fracture rate decreased significantly in the upper extremities (p = 0.04) but not the lower extremities (p = 0.09) during the first year of treatment. Gross motor function, muscle strength, and pain did not change significantly during the controlled or extended treatment phases. Conclusions: A controlled trial confirmed the spine benefits of short-term pamidronate treatment in children with types III and IV OI. Pamidronate increased L sub(1)-L sub(4) vertebral DXA and decreased vertebral compressions and upper extremity fractures. Vertebral measures did not improve during the extended treatment phase. The treatment group did not experience decreased lower extremity long bone fractures, significant improvement in growth, ambulation, muscle strength, or pain. There was substantial variability in individual response to treatment. JF - Journal of Bone and Mineral Research AU - Letocha, AD AU - Cintas, H L AU - Troendle, J F AU - Reynolds, J C AU - Cann, CE AU - Chernoff, E J AU - Hill, S C AU - Gerber, L H AU - Marini, J C AD - Section on Connective Tissue Disorders, BEMB, NICHD, Building 10, Room 9s239, 9000 Rockville Pike, Bethesda, MD 20892, USA, oidoc@helix.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 977 EP - 986 VL - 20 IS - 6 SN - 0884-0431, 0884-0431 KW - Physical Education Index; Calcium & Calcified Tissue Abstracts KW - Osteogenesis imperfecta KW - Bones KW - Mobility KW - Bisphosphonates KW - Pain KW - Motor ability KW - Vertebrae KW - Experience KW - Bone mineral density KW - Degenerative diseases KW - Drugs KW - Muscular strength KW - Growth hormone KW - Growth hormones KW - Muscles KW - Fractures KW - Osteoporosis KW - Height KW - Patients KW - Children KW - Movement KW - X-Ray KW - Strength KW - Spine KW - Pamidronic acid KW - Injections KW - T 200115:Bone pharmacology and toxicology KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17871887?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bone+and+Mineral+Research&rft.atitle=Controlled+Trial+of+Pamidronate+in+Children+With+Types+III+and+IV+Osteogenesis+Imperfecta+Confirms+Vertebral+Gains+but+Not+Short-Term+Functional+Improvement&rft.au=Letocha%2C+AD%3BCintas%2C+H+L%3BTroendle%2C+J+F%3BReynolds%2C+J+C%3BCann%2C+CE%3BChernoff%2C+E+J%3BHill%2C+S+C%3BGerber%2C+L+H%3BMarini%2C+J+C&rft.aulast=Letocha&rft.aufirst=AD&rft.date=2005-06-01&rft.volume=20&rft.issue=6&rft.spage=977&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bone+and+Mineral+Research&rft.issn=08840431&rft_id=info:doi/10.1359%2FJBMR.050109 LA - English DB - Physical Education Index N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Children; Fractures; Pain; Bones; Spine; Strength; Experience; Patients; Height; X-Ray; Drugs; Degenerative diseases; Bone mineral density; Movement; Muscles; Motor ability; Injections; Growth hormones; Bisphosphonates; Vertebrae; Pamidronic acid; Muscular strength; Osteogenesis imperfecta; Growth hormone; Mobility; Osteoporosis DO - http://dx.doi.org/10.1359/JBMR.050109 ER - TY - JOUR T1 - Occupational Bladder Cancer in a 4,4'-Methylenebis(2-chloroaniline) (MBOCA)-Exposed Worker AN - 17656320; 6489046 AB - A 52-year-old male chemical worker was admitted to the hospital with a history of paroxysmal microscopic hematuria for about 2 years and nocturia with gross hematuria about five times per night for 2 months. He was a nonsmoker and denied a history of any other bladder carcinogen exposure except for occasional pesticide application during agricultural work. Intravenous urogram imaging showed a mass occupying half of the bladder capacity. Cystoscopy revealed a mass over the left dome of the bladder. Cystoscopic biopsy revealed a grade 3 invasive transitional cell carcinoma with marked necrosis. From 1987 until hospital admission in 2001, the patient had worked in a company that produced the 4,4'-methylenebis(2- chloroaniline) (MBOCA) curing agent. He did not wear any personal protective equipment during work Ambient air MBOCA levels in the purification process area (0.23-0.41 mg/m super(3)) exceeded the U.S. Occupational Safety and Health Administration's permissible exposure level. Urinary MBOCA levels (267.9-15701.1 mu g/g creatinine) far exceeded the California Occupational Safety and Health Administration's reference value of 100 mu g/L. This patient worked in the purification process with occupational exposure to MBOCA for 14 years. According to the environmental and biologic monitoring data and latency period, and excluding other potential bladder carcinogen exposure, this worker was diagnosed as having occupational bladder cancer due to high exposure to MBOCA through inhalation or dermal absorption in the purification area. This case finding supports that MBOCA is a potential human carcinogen. Safe use of skin- protective equipment and respirators is required to prevent workers from MBOCA exposure. JF - Environmental Health Perspectives AU - Liu, Chiu-Shong AU - Liou, Saou-Hsing AU - Loh, Ching-Hui AU - Yu, Yi-Chun AU - Uang, Shi-Nian AU - Shih, Tung-Sheng AU - Chen, Hong-I AD - Division of Urology, Department of Surgery, Tri-Service General Hospital, 325 Chen-Kung Rd., Section 2, Nei-Hu, Taipei, 114, Taiwan, ROC, hong_i@ndmctsgh.edu.tw Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 771 EP - 774 VL - 113 IS - 6 SN - 0091-6765, 0091-6765 KW - 4,4'-methylenebis(2-chloroaniline) KW - urinary bladder KW - Toxicology Abstracts; Health & Safety Science Abstracts KW - H 1000:Occupational Safety and Health KW - X 24136:Environmental impact UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17656320?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Occupational+Bladder+Cancer+in+a+4%2C4%27-Methylenebis%282-chloroaniline%29+%28MBOCA%29-Exposed+Worker&rft.au=Liu%2C+Chiu-Shong%3BLiou%2C+Saou-Hsing%3BLoh%2C+Ching-Hui%3BYu%2C+Yi-Chun%3BUang%2C+Shi-Nian%3BShih%2C+Tung-Sheng%3BChen%2C+Hong-I&rft.aulast=Liu&rft.aufirst=Chiu-Shong&rft.date=2005-06-01&rft.volume=113&rft.issue=6&rft.spage=771&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7666 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1289/ehp.7666 ER - TY - JOUR T1 - Protective Antigen and Toxin Neutralization Antibody Patterns in Anthrax Vaccinees Undergoing Serial Plasmapheresis AN - 17640489; 6416907 AB - Recipients of licensed anthrax vaccine (AVA; Biothrax) could serve as a source of hyperimmune plasma and immunoglobulin for therapy and prophylaxis. We measured serum antibodies during serial weekly to biweekly plasmapheresis in 38 individuals previously vaccinated with 4 to 27 doses of AVA. Immunoglobulin G (IgG) to protective antigen (PA) and toxin neutralization assay (TNA) antibody levels were highly correlated (r = 0.86930 and P < 0.0001 for anti-PA concentration versus TNA concentration). Significant decreases in antibody titer and concentration were observed over time when compared for the number of days from the last AVA injection (P < 0.0001 for both anti-PA and TNA concentration) and for the number of days from the first plasmapheresis (P = 0.0007 for anti-PA concentration and P = 0.0025 for TNA concentration). The rate of the decrease in total IgG concentration (half-life [t sub(1/2)] = 198.90 days after first plasmapheresis) was significantly less than the decrease in anti-PA IgG (t sub(1/2) = 63.53 days) (P < 0.0001), indicating that the reduction in anti-PA IgG was more likely due to natural decay than plasmapheresis. The time since the last injection and the time after initial plasmapheresis are important elements in considering an optimal schedule for collecting anthrax hyperimmune plasma. Good correlation between IgG to PA and TNA antibodies suggests that the anti-PA enzyme-linked immunosorbent assay can be used as a high-throughput screen for functional immune reactivity in donor plasma units. JF - Clinical and Diagnostic Laboratory Immunology AU - Pittman, Phillip R AU - Leitman, Susan F AU - Oro, Julio GBarrera AU - Norris, Sarah L AU - Marano, Nina M AU - Ranadive, Manmohan V AU - Sink, Bonnie S AU - McKee, Kelly TJr AD - United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702. National Institutes of Health, Bethesda, Maryland 20892. Camber Corporation/USAMRIID, Fort Detrick, Maryland 21702. Goldbelt-Raven/USAMRIID, Fort Detrick, Maryland 21702. Centers for Disease Control and Prevention, Atlanta, Georgia 30333 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 713 EP - 721 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 12 IS - 6 SN - 1071-412X, 1071-412X KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - J 02834:Vaccination and immunization KW - F 06100:Vaccines - active immunity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17640489?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+and+Diagnostic+Laboratory+Immunology&rft.atitle=Protective+Antigen+and+Toxin+Neutralization+Antibody+Patterns+in+Anthrax+Vaccinees+Undergoing+Serial+Plasmapheresis&rft.au=Pittman%2C+Phillip+R%3BLeitman%2C+Susan+F%3BOro%2C+Julio+GBarrera%3BNorris%2C+Sarah+L%3BMarano%2C+Nina+M%3BRanadive%2C+Manmohan+V%3BSink%2C+Bonnie+S%3BMcKee%2C+Kelly+TJr&rft.aulast=Pittman&rft.aufirst=Phillip&rft.date=2005-06-01&rft.volume=12&rft.issue=6&rft.spage=713&rft.isbn=&rft.btitle=&rft.title=Clinical+and+Diagnostic+Laboratory+Immunology&rft.issn=1071412X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Multiple Copies of the 16S rRNA Gene in Nocardia nova Isolates and Implications for Sequence-Based Identification Procedures AN - 17636960; 6427565 AB - Molecular investigation of two Nocardia patient isolates showed unusual restriction fragment length polymorphism patterns with restriction endonuclease assays (REA) using an amplified portion of the 16S rRNA gene. Patterns typical of Nocardia nova were obtained with REA of an amplified portion of the 65-kDa heat shock protein gene. Subsequent sequence analysis of the 16S rRNA gene regions of these isolates showed the presence of ambiguous bases within an expected restriction endonuclease recognition site which were not able to be resolved on repeat testing. Cloning of amplified regions of the 16S rRNA genes and subsequent sequencing of the resulting clones from the two patient isolates showed three different 16S rRNA gene sequences which corresponded to sequences found in N. nova, a molecular variant of N. nova, and a previously undescribed sequence. Hybridization studies using a DNA probe corresponding to an 89-bp conserved region of the 16S rRNA gene confirmed the presence of at least two copies of the 16S rRNA gene in the N. nova type strain, in a patient isolate identical to the molecular variant of N. nova, and in the two other patient isolates. All isolates were found to belong to the species N. nova as determined by DNA-DNA hybridization. Because minimal variation has been found in the 16S rRNA gene sequences of different species of Nocardia, those laboratories employing molecular methods for identification of these species must be aware of the potential identification complications that may be caused by the presence of differing 16S rRNA genes in the same isolate. JF - Journal of Clinical Microbiology AU - Conville, Patricia S AU - Witebsky, Frank G AD - Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2881 EP - 2885 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 6 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02726:RNA and ribosomes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17636960?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Multiple+Copies+of+the+16S+rRNA+Gene+in+Nocardia+nova+Isolates+and+Implications+for+Sequence-Based+Identification+Procedures&rft.au=Conville%2C+Patricia+S%3BWitebsky%2C+Frank+G&rft.aulast=Conville&rft.aufirst=Patricia&rft.date=2005-06-01&rft.volume=43&rft.issue=6&rft.spage=2881&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Self-administration of cannabinoids by experimental animals and human marijuana smokers AN - 17636712; 6452659 AB - Drug self-administration behavior has been one of the most direct and productive approaches for studying the reinforcing effects of psychoactive drugs, which are critical in determining their abuse potential. Cannabinoids, which are usually abused by humans in the form of marijuana, have become the most frequently abused illicit class of drugs in the United States. The early elucidation of the structure and stereochemistry of delta-9-tetrahydrocannabinol (THC) in 1964, which is now recognized as the principal psychoactive ingredient in marijuana, activated cannabinoid research worldwide. This review examines advances in research on cannabinoid self-administration behavior by humans and laboratory animals. There have been numerous laboratory demonstrations of the reinforcing effects of cannabinoids in human subjects, but reliable self-administration of cannabinoids by laboratory animals has only recently been demonstrated. It has now been shown that strong and persistent self-administration behavior can be maintained in experimentally and drug-naive squirrel monkeys by doses of THC comparable to those in marijuana smoke inhaled by humans. Furthermore, reinforcing effects of some synthetic CB sub(1) cannabinoid agonists have been recently reported using intravenous and intracerebroventricular self-administration procedures in rats and mice. These findings support previous conclusions that THC has a pronounced abuse liability comparable to other drugs of abuse under certain experimental conditions. Self-administration of THC by squirrel monkeys provides the most reliable animal model for human marijuana abuse available to date. This animal model now makes it possible to study the relative abuse liability of other natural and synthetic cannabinoids and to preclinically assess new therapeutic strategies for the treatment or prevention of marijuana abuse in humans. JF - Pharmacology Biochemistry and Behavior AU - Justinova, Z AU - Goldberg AU - Heishman, S J AU - Tanda, G AD - Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, MD 21224, USA, gtanda@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 285 EP - 299 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 81 IS - 2 SN - 0091-3057, 0091-3057 KW - Squirrel monkeys KW - Toxicology Abstracts; CSA Neurosciences Abstracts; Animal Behavior Abstracts KW - X 24180:Social poisons & drug abuse KW - Y 25591:General KW - N3 11139:Toxicological and psychoactive drug correlates UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17636712?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacology+Biochemistry+and+Behavior&rft.atitle=Self-administration+of+cannabinoids+by+experimental+animals+and+human+marijuana+smokers&rft.au=Justinova%2C+Z%3BGoldberg%3BHeishman%2C+S+J%3BTanda%2C+G&rft.aulast=Justinova&rft.aufirst=Z&rft.date=2005-06-01&rft.volume=81&rft.issue=2&rft.spage=285&rft.isbn=&rft.btitle=&rft.title=Pharmacology+Biochemistry+and+Behavior&rft.issn=00913057&rft_id=info:doi/10.1016%2Fj.pbb.2005.01.026 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1016/j.pbb.2005.01.026 ER - TY - JOUR T1 - Selection of Strains for Quality Assessment of the Disk Induction Method for Detection of Inducible Clindamycin Resistance in Staphylococci: a CLSI Collaborative Study AN - 17636406; 6427526 AB - A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four strains of Staphylococcus aureus were tested as unknowns on 10 different days in each laboratory using the recently recommended CLSI (formerly NCCLS) disk diffusion method and the inoculum purity control method. Strains contained either macrolide-lincosamide-streptogramin B (MLS sub(B)) resistance genes encoded by erm(A) or erm(C) or a macrolide resistance efflux pump encoded by msr(A). Based upon the results of this study, strain UT 32 (now designated ATCC strain BAA-977) containing erm(A) is recommended as the positive control organism for inducible clindamycin resistance. Strain UT 25 (now designated ATCC BAA-976), which harbors the efflux pump encoded by msr(A), is recommended as the negative control organism. JF - Journal of Clinical Microbiology AU - Zelazny, Adrian M AU - Ferraro, Mary Jane AU - Glennen, Anita AU - Hindler, Janet F AU - Mann, Linda M AU - Munro, Susan AU - Murray, Patrick R AU - Reller, LBarth AU - Tenover, Fred C AU - Jorgensen, James H AD - Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892. Microbiology Laboratory, Clinical Microbiology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114. Acute Disease Epidemiology, Minnesota Department of Health, Minneapolis, Minnesota 55414. Department of Pathology and Laboratory Medicine, University of California at Los Angeles Medical Center, Los Angeles, California 90025. Clinical Microbiology Laboratory, Baylor University Medical Center, Dallas, Texas 75246. Clinical Microbiology Laboratory, Stanford University Medical School, Stanford, California 94305. Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710. Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333. Department of Pathology, University of Texas Health Science Center, San Antonio, Texas 78229 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2613 EP - 2615 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 6 SN - 0095-1137, 0095-1137 KW - Microbiology Abstracts B: Bacteriology KW - J 02704:Enumeration UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17636406?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Selection+of+Strains+for+Quality+Assessment+of+the+Disk+Induction+Method+for+Detection+of+Inducible+Clindamycin+Resistance+in+Staphylococci%3A+a+CLSI+Collaborative+Study&rft.au=Zelazny%2C+Adrian+M%3BFerraro%2C+Mary+Jane%3BGlennen%2C+Anita%3BHindler%2C+Janet+F%3BMann%2C+Linda+M%3BMunro%2C+Susan%3BMurray%2C+Patrick+R%3BReller%2C+LBarth%3BTenover%2C+Fred+C%3BJorgensen%2C+James+H&rft.aulast=Zelazny&rft.aufirst=Adrian&rft.date=2005-06-01&rft.volume=43&rft.issue=6&rft.spage=2613&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Longitudinal analysis of the group A Streptococcus transcriptome in experimental pharyngitis in cynomolgus macaques AN - 17634911; 6426936 AB - Identification of the genetic events that contribute to host-pathogen interactions is important for understanding the natural history of infectious diseases and developing therapeutics. Transcriptome studies conducted on pathogens have been central to this goal in recent years. However, most of these investigations have focused on specific end points or disease phases, rather than analysis of the entire time course of infection. To gain a more complete understanding of how bacterial gene expression changes over time in a primate host, the transcriptome of group A Streptococcus (GAS) was analyzed during an 86-day infection protocol in 20 cynomolgus macaques with experimental pharyngitis. The study used 260 custom Affymetrix (Santa Clara, CA) chips, and data were confirmed by TaqMan analysis. Colonization, acute, and asymptomatic phases of disease were identified. Successful colonization and severe inflammation were significantly correlated with an early onset of superantigen gene expression. The differential expression of two-component regulators covR and spy0680 (M1_spy0874) was significantly associated with GAS colony-forming units, inflammation, and phases of disease. Prophage virulence gene expression and prophage induction occurred predominantly during high pathogen cell densities and acute inflammation. We discovered that temporal changes in the GAS transcriptome were integrally linked to the phase of clinical disease and host-defense response. Knowledge of the gene expression patterns characterizing each phase of pathogen-host interaction provides avenues for targeted investigation of proven and putative virulence factors and genes of unknown function and will assist vaccine research. JF - Proceedings of the National Academy of Sciences, USA AU - Virtaneva, Kimmo AU - Porcella, Stephen F AU - Graham, Morag R AU - Ireland, Robin M AU - Johnson, Claire A AU - Ricklefs, Stacy M AU - Babar, Imran AU - Parkins, Larye D AU - Romero, Romina A AU - Corn, GJudson AU - Gardner, Don J AU - Bailey, John R AU - Parnell, Michael J AU - Musser, James M AD - Laboratory of Human Bacterial Pathogenesis and Veterinary Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 9014 EP - 9019 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 25 SN - 0027-8424, 0027-8424 KW - Macaques KW - primates KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - J 02855:Human Bacteriology: Others KW - N 14025:RNA/DNA role in infection & immune response UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17634911?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Longitudinal+analysis+of+the+group+A+Streptococcus+transcriptome+in+experimental+pharyngitis+in+cynomolgus+macaques&rft.au=Virtaneva%2C+Kimmo%3BPorcella%2C+Stephen+F%3BGraham%2C+Morag+R%3BIreland%2C+Robin+M%3BJohnson%2C+Claire+A%3BRicklefs%2C+Stacy+M%3BBabar%2C+Imran%3BParkins%2C+Larye+D%3BRomero%2C+Romina+A%3BCorn%2C+GJudson%3BGardner%2C+Don+J%3BBailey%2C+John+R%3BParnell%2C+Michael+J%3BMusser%2C+James+M&rft.aulast=Virtaneva&rft.aufirst=Kimmo&rft.date=2005-06-01&rft.volume=102&rft.issue=25&rft.spage=9014&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Enhanced Immunogenicity of gp120 Protein When Combined with Recombinant DNA Priming To Generate Antibodies That Neutralize the JR-FL Primary Isolate of Human Immunodeficiency Virus Type 1 AN - 17628278; 6415473 AB - Strategies are needed for human immunodeficiency virus type 1 vaccine development that improves the neutralizing antibody response against primary isolates of the virus. Here we examined recombinant DNA priming followed by subunit protein boosting as a strategy to generate neutralizing antibodies. Both plasmid-based and recombinant protein envelope (Env) glycoprotein immunogens were derived from a primary viral isolate, JR-FL. Serum from rabbits immunized with either gp120 or gp140 DNA vaccines delivered by gene gun inoculation followed by recombinant gp120 protein boosting was capable of neutralizing JR-FL. Neither the DNA vaccines alone nor the gp120 protein alone generated a detectable neutralizing antibody response against this virus. Neutralizing antibody responses using gp120 DNA and gp140 DNA for priming were similar. The results suggest that Env DNA priming followed by gp120 protein boosting provides an advantage over either approach alone for generating a detectable neutralizing antibody response against primary isolates that are not easily neutralized. JF - Journal of Virology AU - Wang, Shixia AU - Arthos, James AU - Lawrence, John M AU - Van Ryk, Donald AU - Mboudjeka, Innocent AU - Shen, Siyuan AU - Chou, Te-hui W AU - Montefiori, David C AU - Lu, Shan AD - Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605. Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892. Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 7933 EP - 7937 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 12 SN - 0022-538X, 0022-538X KW - HIV-1 KW - glycoprotein gp140 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Virology & AIDS Abstracts KW - W3 33375:Antibodies KW - N 14025:RNA/DNA role in infection & immune response KW - V 22003:AIDS: Immunological aspects KW - W 30965:Miscellaneous, Reviews KW - F 06104:Virus UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17628278?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Enhanced+Immunogenicity+of+gp120+Protein+When+Combined+with+Recombinant+DNA+Priming+To+Generate+Antibodies+That+Neutralize+the+JR-FL+Primary+Isolate+of+Human+Immunodeficiency+Virus+Type+1&rft.au=Wang%2C+Shixia%3BArthos%2C+James%3BLawrence%2C+John+M%3BVan+Ryk%2C+Donald%3BMboudjeka%2C+Innocent%3BShen%2C+Siyuan%3BChou%2C+Te-hui+W%3BMontefiori%2C+David+C%3BLu%2C+Shan&rft.aulast=Wang&rft.aufirst=Shixia&rft.date=2005-06-01&rft.volume=79&rft.issue=12&rft.spage=7933&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Identification of nine human-specific frameshift mutations by comparative analysis of the human and the chimpanzee genome sequences AN - 17582274; 6429081 AB - MOTIVATION: The recent release of the draft sequence of the chimpanzee genome is an invaluable resource for finding genome-wide genetic differences that might explain phenotypic differences between humans and chimpanzees. RESULTS: In this paper, we describe a simple procedure to identify potential human-specific frameshift mutations that occurred after the divergence of human and chimpanzee. The procedure involves collecting human coding exons bearing insertions or deletions compared with the chimpanzee genome and identification of homologs from other species, in support of the mutations being human-specific. Using this procedure, we identified nine genes, BASE, DNAJB3, FLJ33674, HEJ1, NTSR2, RPL13AP, SCGB1D4, WBSCR27 and ZCCHC13, that show human-specific alterations including truncations of the C-terminus. In some cases, the frameshift mutation results in gene inactivation or decay. In other cases, the altered protein seems to be functional. This study demonstrates that even the unfinished chimpanzee genome sequence can be useful in identifying modification of genes that are specific to the human lineage and, therefore, could potentially be relevant to the study of the acquisition of human-specific traits. AVAILABILITY: JF - Bioinformatics AU - Hahn, Yoonsoo AU - Lee, Byungkook AD - Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - i186 EP - i194 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Genomes KW - Deletion KW - Frameshift mutation KW - Insertion KW - Exons KW - Nucleotide sequence KW - C-Terminus KW - Bioinformatics KW - W4 140:Bioinformatics & Computers in Health & Medicine KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17582274?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Identification+of+nine+human-specific+frameshift+mutations+by+comparative+analysis+of+the+human+and+the+chimpanzee+genome+sequences&rft.au=Hahn%2C+Yoonsoo%3BLee%2C+Byungkook&rft.aulast=Hahn&rft.aufirst=Yoonsoo&rft.date=2005-06-01&rft.volume=21&rft.issue=&rft.spage=i186&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Genomes; Frameshift mutation; Nucleotide sequence; Exons; Deletion; C-Terminus; Insertion; Bioinformatics ER - TY - JOUR T1 - MEDS and PocR are novel domains with a predicted role in sensing simple hydrocarbon derivatives in prokaryotic signal transduction systems AN - 17579359; 6417124 AB - We identify two conserved domains in diverse bacterial and archaeal signaling proteins. One of them, the MEDS domain, is typified by the DmcR protein from Methylococcus and the other by the PocR protein of Salmonella typhi. We provide evidence that both these domains are likely to sense simple hydrocarbon derivatives and transduce downstream signals on binding these ligands. The PocR ligand-binding domain is shown to contain a novel variant of the fold found in PAS and GAF domains. The MEDS domain is present in both methylotrophs and complex methanogens, and both the MEDS and PocR domains show a lineage-specific expansion in the latter organisms, suggesting a role in sensing their principle growth substrates. The MEDS domain is also found in the negative regulators of the sigma factor SigB in actinomycetes, including pathogens like Mycobacterium tuberculosis. Hence it is possible that these sigma factors, involved in aerial mycelium development and stress response in the actinomycetes, might be under the regulation of as yet uncharacterized small molecules. JF - Bioinformatics AU - Anantharaman, Vivek AU - Aravind, L AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Bethesda, MD 20894, USA Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2805 EP - 2811 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 12 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Hydrocarbons KW - Salmonella typhi KW - Stress KW - Bioinformatics KW - Pathogens KW - Methanogenic bacteria KW - Sigma factor KW - Actinomycetes KW - Signal transduction KW - Methylococcus KW - Mycobacterium tuberculosis KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17579359?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=MEDS+and+PocR+are+novel+domains+with+a+predicted+role+in+sensing+simple+hydrocarbon+derivatives+in+prokaryotic+signal+transduction+systems&rft.au=Anantharaman%2C+Vivek%3BAravind%2C+L&rft.aulast=Anantharaman&rft.aufirst=Vivek&rft.date=2005-06-01&rft.volume=21&rft.issue=12&rft.spage=2805&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; Methylococcus; Salmonella typhi; Sigma factor; Signal transduction; Actinomycetes; Hydrocarbons; Methanogenic bacteria; Pathogens; Stress; Bioinformatics ER - TY - JOUR T1 - Differential Activities of Plant Polyphenols on the Binding and Internalization of Cholera Toxin in Vero Cells AN - 17543552; 6414332 AB - Plant polyphenols, RG-tannin, and applephenon had been reported to inhibit cholera toxin (CT) ADP-ribosyltransferase activity and CT-induced fluid accumulation in mouse ileal loops. A high molecular weight fraction of hop bract extract (HBT) also inhibited CT ADP-ribosyltransferase activity. We report here the effect of those polyphenols on the binding and entry of CT into Vero cells. Binding of CT to Vero cells or to ganglioside GM1, a CT receptor, was inhibited in a concentration-dependent manner by HBT and applephenon but not RG-tannin. These observations were confirmed by fluorescence microscopy using Cy3-labeled CT. Following toxin binding to cells, applephenon, HBT, and RG-tannin suppressed its internalization. HBT or applephenon precipitated CT, CTA, and CTB from solution, creating aggregates larger than 250 kDa. In contrast, RG-tannin precipitated CT poorly; it formed complexes with CT, CTA, or CTB, which were demonstrated with sucrose density gradient centrifugation and molecular weight exclusion filters. In agreement, CTA blocked the inhibition of CT internalization by RG-tannin. These data suggest that some plant polyphenols, similar to applephenon and HBT, bind CT, forming large aggregates in solution or, perhaps, on the cell surface and thereby suppress CT binding and internalization. In contrast, RG-tannin binding to CT did not interfere with its binding to Vero cells or GM1, but it did inhibit internalization. JF - Journal of Biological Chemistry AU - Morinaga, Naoko AU - Iwamaru, Yoshifumi AU - Yahiro, Kinnosuke AU - Tagashira, Motoyuki AU - Moss, Joel AU - Noda, Masatoshi AD - Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan, Fundamental Research Laboratory, Asahi Breweries, Ltd., 1-1-21, Midori, Moriya, Ibaraki, 302-0106, Japan, and Pulmonary-Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-1590 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 23303 EP - 23309 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 24 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Filters KW - Ganglioside GM1 KW - Centrifugation KW - Cell surface KW - ADP-ribosyltransferase KW - Fluorescence KW - Density gradients KW - Vero cells KW - Polyphenols KW - Cholera toxin KW - Molecular weight KW - J 02823:In vitro and in vivo effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17543552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Differential+Activities+of+Plant+Polyphenols+on+the+Binding+and+Internalization+of+Cholera+Toxin+in+Vero+Cells&rft.au=Morinaga%2C+Naoko%3BIwamaru%2C+Yoshifumi%3BYahiro%2C+Kinnosuke%3BTagashira%2C+Motoyuki%3BMoss%2C+Joel%3BNoda%2C+Masatoshi&rft.aulast=Morinaga&rft.aufirst=Naoko&rft.date=2005-06-01&rft.volume=280&rft.issue=24&rft.spage=23303&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Polyphenols; Vero cells; ADP-ribosyltransferase; Cholera toxin; Cell surface; Molecular weight; Ganglioside GM1; Density gradients; Fluorescence; Centrifugation; Filters ER - TY - JOUR T1 - Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes AN - 17538909; 6406530 AB - The highly conserved, atypical RIO serine protein kinases are found in all organisms, from archaea to man. In yeast, the kinase activity of Rio2 is necessary for the final processing step of maturing the 18S ribosomal rRNA. We have previously shown that the Rio2 protein from Archaeoglobus fulgidus contains both a small kinase domain and an N-terminal winged helix domain. Previously solved structures using crystals soaked in nucleotides and Mg super(2+) or Mn super(2+) showed bound nucleotide but no ordered metal ions, leading us to the conclusion that they did not represent an active conformation of the enzyme. To determine the functional form of Rio2, we crystallized it after incubation with ATP or ADP and Mn super(2+). Co-crystal structures of Rio2-ATP-Mn and Rio2-ADP-Mn were solved at 1.84 and 1.75 Aa resolution, respectively. The gamma -phosphate of ATP is firmly positioned in a manner clearly distinct from its location in canonical serine kinases. Comparison of the Rio2-ATP-Mn complex with the Rio2 structure with no added nucleotides and with the ADP complex indicates that a flexible portion of the Rio2 molecule becomes ordered through direct interaction between His126 and the gamma -phosphate oxygen of ATP. Phosphopeptide mapping of the autophosphorylation site of Rio2 identified Ser128, within the flexible loop and directly adjacent to the part that becomes ordered in response to ATP, as the target. These results give us further information about the nature of the active site of Rio2 kinase and suggest a mechanism of regulation of its enzymatic activity. JF - FEBS Journal AU - LaRonde-LeBlanc, Nicole AU - Guszczynski, Tad AU - Copeland, Terry AU - Wlodawer, Alexander AD - Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI-Frederick, MD USA, wlodawer@ncifcrf.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2800 EP - 2810 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 272 IS - 11 SN - 1742-464X, 1742-464X KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - rRNA 18S KW - Metals KW - Ions KW - protein-serine kinase KW - Archaea KW - Archaeoglobus fulgidus KW - Enzymes KW - ATP KW - Crystals KW - Nucleotides KW - Oxygen KW - Crystal structure KW - Protein kinase KW - Mapping KW - Enzymatic activity KW - Magnesium KW - N 14070:Ribosomes: rRNA, ribosomal proteins, and translation KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17538909?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Journal&rft.atitle=Autophosphorylation+of+Archaeoglobus+fulgidus+Rio2+and+crystal+structures+of+its+nucleotide-metal+ion+complexes&rft.au=LaRonde-LeBlanc%2C+Nicole%3BGuszczynski%2C+Tad%3BCopeland%2C+Terry%3BWlodawer%2C+Alexander&rft.aulast=LaRonde-LeBlanc&rft.aufirst=Nicole&rft.date=2005-06-01&rft.volume=272&rft.issue=11&rft.spage=2800&rft.isbn=&rft.btitle=&rft.title=FEBS+Journal&rft.issn=1742464X&rft_id=info:doi/10.1111%2Fj.1742-4658.2005.04702.x LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Figures, 6; tables, 1; references, 27. N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - rRNA 18S; Ions; Metals; protein-serine kinase; ATP; Enzymes; Crystals; Nucleotides; Oxygen; Crystal structure; Protein kinase; Enzymatic activity; Mapping; Magnesium; Archaea; Archaeoglobus fulgidus DO - http://dx.doi.org/10.1111/j.1742-4658.2005.04702.x ER - TY - JOUR T1 - Modification of liposomal concentration in liposome/adenoviral complexes allows significant protection of adenoviral vectors from neutralising antibody, in vitro AN - 17537617; 6391478 AB - Adenoviral vectors have been commonly used in gene therapy protocols, however the success of their use is often limited by the induction of host immunity to the vector. Following exposure to the adenoviral vector, adenoviral-specific neutralising antibodies are produced which limits further administration. This study examines the efficacy of complexing liposomes to adenovirus for the protection of the adenovirus from neutralising antibodies in an in vitro setting. Dimethyldioctadecylammonium bromide (DDAB)-dioleoyl-l-phosphatidylethanolamine (DOPE) liposomes were bound at varying concentrations to adenovirus to form AL complexes and tested these complexes' ability to prevent adenoviral neutralisation. It is shown that by increasing the concentration of liposomes in the adenoviral-liposome (AL) complexes we can increase the level of immuno-shielding afforded the adenovirus. It is also shown that the increase in liposomal concentration may lead to drawbacks such as increased cytotoxicity and reductions in expression levels. JF - Journal of Virological Methods AU - Steel, J C AU - Cavanagh, HMA AU - Burton, MA AU - Dingwall, D J AU - Kalle, WHJ AD - Charles Sturt University, P.O. Box 588, Wagga Wagga 2678, Australia, steeljas@mail.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 31 EP - 36 PB - Elsevier B.V. VL - 126 IS - 1-2 SN - 0166-0934, 0166-0934 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Bioengineering Abstracts; Virology & AIDS Abstracts KW - Gene therapy KW - Adenovirus KW - Immunity KW - Liposomes KW - Expression vectors KW - Cytotoxicity KW - Antibodies KW - W3 33181:Gene therapy vectors KW - V 22093:Antigen-antibody interaction KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17537617?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virological+Methods&rft.atitle=Modification+of+liposomal+concentration+in+liposome%2Fadenoviral+complexes+allows+significant+protection+of+adenoviral+vectors+from+neutralising+antibody%2C+in+vitro&rft.au=Steel%2C+J+C%3BCavanagh%2C+HMA%3BBurton%2C+MA%3BDingwall%2C+D+J%3BKalle%2C+WHJ&rft.aulast=Steel&rft.aufirst=J&rft.date=2005-06-01&rft.volume=126&rft.issue=1-2&rft.spage=31&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virological+Methods&rft.issn=01660934&rft_id=info:doi/10.1016%2Fj.jviromet.2005.01.017 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adenovirus; Expression vectors; Liposomes; Antibodies; Cytotoxicity; Immunity; Gene therapy DO - http://dx.doi.org/10.1016/j.jviromet.2005.01.017 ER - TY - JOUR T1 - Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response AN - 17502490; 6265681 AB - We examined Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to M. penetrans isolation reacted with a number of M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of M. penetrans with molecular mass of 35 and 38kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for M. penetrans infection. JF - FEMS Immunology and Medical Microbiology AU - Lo, S-C AU - Wang, RY-H AU - Grandinetti, T AU - Zou, N AU - Hayes, M M AU - Shih, JW-K AU - Wear, D J AD - Department of Transfusion Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD, USA, los@afip.osd.mil Y1 - 2005/06/01/ PY - 2005 DA - 2005 Jun 01 SP - 277 EP - 282 VL - 44 IS - 3 SN - 0928-8244, 0928-8244 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts KW - Acquired immune deficiency syndrome KW - Cross-reaction KW - Membrane proteins KW - Infection KW - Seroconversion KW - Mycoplasma penetrans KW - Western blotting KW - Antibody response KW - Clinical microbiology KW - F 06308:Clinical: AIDS KW - J 02855:Human Bacteriology: Others KW - V 22004:AIDS: Clinical aspects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17502490?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEMS+Immunology+and+Medical+Microbiology&rft.atitle=Mycoplasma+penetrans+infections+and+seroconversion+in+patients+with+AIDS%3A+identification+of+major+mycoplasmal+antigens+targeted+by+host+antibody+response&rft.au=Lo%2C+S-C%3BWang%2C+RY-H%3BGrandinetti%2C+T%3BZou%2C+N%3BHayes%2C+M+M%3BShih%2C+JW-K%3BWear%2C+D+J&rft.aulast=Lo&rft.aufirst=S-C&rft.date=2005-06-01&rft.volume=44&rft.issue=3&rft.spage=277&rft.isbn=&rft.btitle=&rft.title=FEMS+Immunology+and+Medical+Microbiology&rft.issn=09288244&rft_id=info:doi/10.1016%2Fj.femsim.2004.12.010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycoplasma penetrans; Acquired immune deficiency syndrome; Seroconversion; Clinical microbiology; Western blotting; Antibody response; Cross-reaction; Membrane proteins; Infection DO - http://dx.doi.org/10.1016/j.femsim.2004.12.010 ER - TY - JOUR T1 - A low-carb diet for a high-octane pathogen AN - 17500456; 6263376 AB - Mycobacterium tuberculosis adapts to the low-glucose conditions in its host by using lipids as a fuel source. This adaptation reveals a weak flank that might be exploited in drug development, as shown in work on mice and human cells. JF - Nature Medicine AU - Boshoff, H I AU - Barry, CE III AD - Tuberculosis Research Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Rockville, Maryland 20852, USA, cbarry@niaid.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 599 EP - 600 VL - 11 IS - 6 SN - 1078-8956, 1078-8956 KW - Microbiology Abstracts B: Bacteriology KW - Adaptations KW - Lipids KW - Glucose KW - Drug development KW - Pathogens KW - Mycobacterium tuberculosis KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17500456?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Medicine&rft.atitle=A+low-carb+diet+for+a+high-octane+pathogen&rft.au=Boshoff%2C+H+I%3BBarry%2C+CE+III&rft.aulast=Boshoff&rft.aufirst=H&rft.date=2005-06-01&rft.volume=11&rft.issue=6&rft.spage=599&rft.isbn=&rft.btitle=&rft.title=Nature+Medicine&rft.issn=10788956&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; Adaptations; Pathogens; Drug development; Lipids; Glucose ER - TY - JOUR T1 - Transcriptional takeover by sigma appropriation: Remodelling of the sigma super(70) subunit of Escherichia coli RNA polymerase by the bacteriophage T4 activator MotA and co-activator AsiA AN - 17494779; 6278951 AB - Activation of bacteriophage T4 middle promoters, which occurs about 1 min after infection, uses two phage-encoded factors that change the promoter specificity of the host RNA polymerase. These phage factors, the MotA activator and the AsiA co-activator, interact with the sigma super(70) specificity subunit of Escherichia coli RNA polymerase, which normally contacts the -10 and -35 regions of host promoter DNA. Like host promoters, T4 middle promoters have a good match to the canonical sigma super(70) DNA element located in the -10 region. However, instead of the sigma super(70) DNA recognition element in the promoter's -35 region, they have a 9 bp sequence (a MotA box) centred at -30, which is bound by MotA. Recent work has begun to provide information about the MotA/AsiA system at a detailed molecular level. Accumulated evidence suggests that the presence of MotA and AsiA reconfigures protein-DNA contacts in the upstream promoter sequences, without significantly affecting the contacts of sigma super(70) with the -10 region. This type of activation, which is called ` sigma appropriation', is fundamentally different from other well-characterized models of prokaryotic activation in which an activator frequently serves to force sigma super(70) to contact a less than ideal -35 DNA element. This review summarizes the interactions of AsiA and MotA with sigma super(70), and discusses how these interactions accomplish the switch to T4 middle promoters by inhibiting the typical contacts of the C-terminal region of sigma super(70), region 4, with the host-35 DNA element and with other subunits of polymerase. JF - Microbiology AU - Hinton, D M AU - Pande, S AU - Wais, N AU - Johnson, X B AU - Vuthoori, M AU - Makela, A AU - Hook-Barnard, I AD - National Oceanic and Atmospheric Administration, Silver Spring, MD, USA, dhinton@helix.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1729 EP - 1740 VL - 151 IS - 6 SN - 1350-0872, 1350-0872 KW - AsiA protein KW - Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology KW - Phages KW - Nucleotide sequence KW - Transcription KW - MotA protein KW - Models KW - Promoters KW - DNA-directed RNA polymerase KW - Reviews KW - Escherichia coli KW - J 02750:Phage-host interactions KW - V 22070:Phage-host interactions including lysogeny & transduction UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17494779?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Microbiology&rft.atitle=Transcriptional+takeover+by+sigma+appropriation%3A+Remodelling+of+the+sigma+super%2870%29+subunit+of+Escherichia+coli+RNA+polymerase+by+the+bacteriophage+T4+activator+MotA+and+co-activator+AsiA&rft.au=Hinton%2C+D+M%3BPande%2C+S%3BWais%2C+N%3BJohnson%2C+X+B%3BVuthoori%2C+M%3BMakela%2C+A%3BHook-Barnard%2C+I&rft.aulast=Hinton&rft.aufirst=D&rft.date=2005-06-01&rft.volume=151&rft.issue=6&rft.spage=1729&rft.isbn=&rft.btitle=&rft.title=Microbiology&rft.issn=13500872&rft_id=info:doi/10.1099%2Fmic.0.27972-0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Promoters; Phages; DNA-directed RNA polymerase; Models; Reviews; MotA protein; Transcription; Nucleotide sequence DO - http://dx.doi.org/10.1099/mic.0.27972-0 ER - TY - JOUR T1 - Involvement of mu-, delta- and kappa-opioid receptor subtypes in the discriminative-stimulus effects of delta-9-tetrahydrocannabinol (THC) in rats AN - 17466742; 6649859 AB - Rationale: Many behavioral effects of delta-9-tetrahydrocannabinol (THC), including its discriminative-stimulus effects, are modulated by endogenous opioid systems. Objective: To investigate opioid receptor subtypes involved in the discriminative effects of THC. Methods: Rats trained to discriminate 3 mg/kg i.p. of THC from vehicle using a two-lever operant drug-discrimination procedure, were tested with compounds that bind preferentially or selectively to either mu-, delta- or kappa-opioid receptors. Results: The preferential mu-opioid receptor agonist heroin (0.3-1.0 mg/kg, i.p.), the selective delta-opioid receptor agonist SNC-80 (1-10 mg/kg, i.p.) and the selective kappa-opioid receptor agonist U50488 (1-10 mg/kg, i.p.) did not produce generalization to the discriminative effects of THC when given alone. However, heroin, but not SNC-80 or U50488, significantly shifted the dose-response curve for THC discrimination to the left. Also, the preferential mu-opioid receptor antagonist naltrexone (0.1-1 mg/kg, i.p.), the selective delta-opioid receptor antagonist, naltrindole (1-10 mg/kg, i.p.) and the kappa-opioid receptor antagonist nor-binaltorphimine (n-BNI, 5 mg/kg, s.c.), did not significantly reduce the discriminative effects of the training dose of THC. However, naltrexone, but not naltrindole or n-BNI, significantly shifted the dose-response curve for THC discrimination to the right. Finally, naltrexone, but not naltrindole or n-BNI, blocked the leftward shift in the dose-response curve for THC discrimination produced by heroin. Conclusions: mu- but not delta- or kappa-opioid receptors are involved in the discriminative effects of THC. Given the role that mu-opioid receptors play in THC's rewarding effects, the present findings suggest that discriminative-stimulus effects and rewarding effects of THC involve similar neural mechanisms. JF - Psychopharmacology AU - Solinas, Marcello AU - Goldberg, Steven R AD - National Institute of Health, Room 318, 5500 Nathan Shock Drive, Baltimore, MD, 21224, USA, msolinas@intra.nida.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 804 EP - 812 PB - Springer-Verlag (Berlin), Heidelberger Platz 3 Berlin 14197 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 179 IS - 4 SN - 0033-3158, 0033-3158 KW - Opioid receptors (type Kappa ) KW - rats KW - CSA Neurosciences Abstracts; Animal Behavior Abstracts; Toxicology Abstracts KW - Tetrahydrocannabinol KW - Discriminative stimuli KW - Naltrindole KW - Heroin KW - Drug discrimination KW - Opioid receptors (type mu ) KW - Naltrexone KW - Opioid receptors (type delta ) KW - Operant response KW - Y 25617:Mammals (excluding primates) KW - X 24180:Social poisons & drug abuse KW - N3 11139:Toxicological and psychoactive drug correlates UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17466742?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Involvement+of+mu-%2C+delta-+and+kappa-opioid+receptor+subtypes+in+the+discriminative-stimulus+effects+of+delta-9-tetrahydrocannabinol+%28THC%29+in+rats&rft.au=Solinas%2C+Marcello%3BGoldberg%2C+Steven+R&rft.aulast=Solinas&rft.aufirst=Marcello&rft.date=2005-06-01&rft.volume=179&rft.issue=4&rft.spage=804&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/10.1007%2Fs00213-004-2118-x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Discriminative stimuli; Tetrahydrocannabinol; Naltrindole; Heroin; Drug discrimination; Naltrexone; Opioid receptors (type mu ); Opioid receptors (type delta ); Operant response DO - http://dx.doi.org/10.1007/s00213-004-2118-x ER - TY - JOUR T1 - Feasibility of Using Subject-Collected Dust Samples in Epidemiologic and Clinical Studies of Indoor Allergens AN - 17381014; 6489031 AB - Studies of indoor allergen exposures are often limited by the cost and logistics of sending technicians to homes to collect dust. In this study we evaluated the feasibility of having subjects collect their own dust samples. The objectives were to compare allergen concentrations between subject- and technician-collected samples and to examine the sample return rate. Using a dust collection device and written instructions provided to them by mail, 102 subjects collected a combined dust sample from a bed and bedroom floor. Later the same day, a technician collected a side-by-side sample. Dust samples were weighed and analyzed for the cat allergen Fel d 1 and the dust mite allergen Der p 1. Fifty additional subjects who were enrolled by telephone were mailed dust collection packages and asked to return a dust sample and questionnaire by mail. A technician did not visit their homes. Correlations between subject- and technician-collected samples were strong for concentrations of Fel d 1 (r = 0.88) and Der p 1 (r = 0.87). With allergen concentrations dichotomized at lower limits of detection and clinically relevant thresholds, agreements between methodologies ranged from 91 to 98%. Although dust weights were correlated (r = 0.48, p < 0.001), subjects collected lighter samples. Among the group of 50 subjects, 46 returned a dust sample and completed questionnaire. The median number of days to receive a sample was 15. With some limitations, subject-collected dust sampling appears to be a valid and practical option for epidemiologic and clinical studies that report allergen concentration as a measure of exposure. JF - Environmental Health Perspectives AU - Arbes, SJ Jr AU - Sever, M AU - Vaughn, B AU - Mehta, J AU - Lynch, J T AU - Mitchell, H AU - Hoppin, JA AU - Spencer, H L AU - Sandler, D P AU - Zeldin, D C AD - NIEHS/NIH, P.O. Box 12233, MD D2-01, Research Triangle Park, NC 27709, USA, zeldin@niehs.nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 665 EP - 669 VL - 113 IS - 6 SN - 0091-6765, 0091-6765 KW - Pollution Abstracts KW - Feasibility studies KW - Allergens KW - Indoor air pollution KW - Air sampling KW - Dust KW - P 0000:AIR POLLUTION UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17381014?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Feasibility+of+Using+Subject-Collected+Dust+Samples+in+Epidemiologic+and+Clinical+Studies+of+Indoor+Allergens&rft.au=Arbes%2C+SJ+Jr%3BSever%2C+M%3BVaughn%2C+B%3BMehta%2C+J%3BLynch%2C+J+T%3BMitchell%2C+H%3BHoppin%2C+JA%3BSpencer%2C+H+L%3BSandler%2C+D+P%3BZeldin%2C+D+C&rft.aulast=Arbes&rft.aufirst=SJ&rft.date=2005-06-01&rft.volume=113&rft.issue=6&rft.spage=665&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7648 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Feasibility studies; Indoor air pollution; Allergens; Air sampling; Dust DO - http://dx.doi.org/10.1289/ehp.7648 ER - TY - JOUR T1 - Virulent Coxiella burnetii does not activate human dendritic cells: Role of lipopolysaccharide as a shielding molecule AN - 17364327; 6426882 AB - Coxiella burnetii is an obligate intracellular bacterium and the etiological agent of the zoonotic disease Q fever. Acute human Q fever is characterized by flu-like symptoms that, in some cases, can result in a persistent infection that may reactivate months or years after initial exposure. Mechanisms by which this obligate parasite evades clearance by the host immune response during persistent infection are unknown. Here, we characterized the interaction of C. burnetii with dendritic cells (DC), critical components of both innate and adaptive immunity. Human DC were infected with two isogenic C. burnetii strains that differ in LPS length. Infection by the Nine Mile phase I (NMI) strain, which is fully virulent and produces full-length LPS, did not result in DC maturation. In contrast, infection by the avirulent Nine Mile phase II strain, producing a severely truncated LPS, resulted in toll-like receptor 4-independent DC maturation and approximately 10-fold more IL-12 and TNF production. NMI did not actively inhibit DC maturation as NMI-infected DC subsequently matured if treated with Escherichia coli LPS or Nine Mile phase II. Furthermore, removal of LPS from NMI dramatically increased its ability to stimulate DC. We propose a model whereby LPS of virulent C. burnetii masks toll-like receptor ligands from innate immune recognition by DC, thereby allowing replication without significant maturation or inflammatory cytokine production. This immune evasion strategy may allow C. burnetii to persist in an immunocompetent host. JF - Proceedings of the National Academy of Sciences, USA AU - Shannon, Jeffrey G AU - Howe, Dale AU - Heinzen, Robert A AD - Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840 Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 8722 EP - 8727 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 24 SN - 0027-8424, 0027-8424 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17364327?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Virulent+Coxiella+burnetii+does+not+activate+human+dendritic+cells%3A+Role+of+lipopolysaccharide+as+a+shielding+molecule&rft.au=Shannon%2C+Jeffrey+G%3BHowe%2C+Dale%3BHeinzen%2C+Robert+A&rft.aulast=Shannon&rft.aufirst=Jeffrey&rft.date=2005-06-01&rft.volume=102&rft.issue=24&rft.spage=8722&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Ultrasound Contrast Media in the Study of Salivary Gland Tumors AN - 17356313; 6421819 AB - The aims of the present study were: to assess standard ultrasound and color-Doppler patterns for the study and characterization of salivary gland tumors; to define the improvement in the color-Doppler ultrasound image after injection of a signal amplifier (Levovist); to compare morphological and vascular aspects of the neoplasm with data obtained during postoperative histopathological analysis. We used color-Doppler ultrasound before and after injection of the contrast medium to study 56 patients (32 males and 24 females) between the ages of 28 and 77 years old, presenting nodular pathology of the salivary glands. Only patients with less than optimal results of the basic color-Doppler examination (grade of vascularization 0-1) were included in the study. The data were then compared with the final histological results. According to B-mode ultrasonography, all neoplasms were hypoechogenous; 8 showed irregular margins and, of these, 6 were carcinomas. We did not obtain significant data regarding size and echostructure. In 10 cases, we observed the presence of multiple locoregional formations in the lymph nodes. Patients were selected on the basis of unsatisfactory color-Doppler examination results; following injection of the contrast medium, we were able to visualize an increased number of vascular signals in 40 out of 56 patients, compared to the basic examination. This enabled us to better assess the vascular map of the neoplasms, as well as more easily trace the vascular blood flow and perform a better extrapolation of the quantitative and semi-quantitative data. This study demonstrated the importance of the vascular pattern, which is predictive of a malignant lesion when it is high-grade with multiple vascular poles and irregular vascular distribution. Furthermore, certain semi-quantitative parameters proved to be important, particularly the velocity of the systolic peak (SP), the cut-off of which was approximately 25 cm/s. The resistance index (RI) and pulsality index (PI) did not prove significant and showed overlapping values. We observed that the contrast medium was useful in characterizing lesions, of the salivary glands by allowing for a more precise vascular map of the lesions, as well as greater diagnostic accuracy in tracing the vascular blood flow and calculating quantitative and semi-quantitative data. JF - Anticancer Research AU - Gallipoli, A AU - Manganella, G AU - de Lutio di Castelguidone, E AU - Mastro, A AU - Ionna, F AU - Pezzullo, L AU - Vallone, P AD - Interventional Ultrasound, National Cancer Institute-Naples, Via M. Semmola-80131, Naples, Italy, vallone.paolo@libero.it Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 2477 EP - 2482 VL - 25 IS - 3C SN - 0250-7005, 0250-7005 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17356313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+Research&rft.atitle=Ultrasound+Contrast+Media+in+the+Study+of+Salivary+Gland+Tumors&rft.au=Gallipoli%2C+A%3BManganella%2C+G%3Bde+Lutio+di+Castelguidone%2C+E%3BMastro%2C+A%3BIonna%2C+F%3BPezzullo%2C+L%3BVallone%2C+P&rft.aulast=Gallipoli&rft.aufirst=A&rft.date=2005-06-01&rft.volume=25&rft.issue=3C&rft.spage=2477&rft.isbn=&rft.btitle=&rft.title=Anticancer+Research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Common Leptin Receptor Polymorphisms do not Modify the Effect of Alcohol Ingestion on Serum Leptin Levels in a Controlled Feeding and Alcohol Ingestion Study AN - 17355793; 6416884 AB - We explored whether serum leptin response to alcohol ingestion was related to common leptin receptor gene polymorphisms, K109R (Lys super(109)Arg), Q223R (Gln super(223)Arg), S343S [Ser(T) super(343)Ser(C)], and K656N (Lys super(656)Asn), of reported physiologic significance during a controlled intervention. Fifty-three participants rotated through three 8-week treatment periods and consumed 0, 15 (equivalent to one drink), or 30 g (equivalent to two drinks) of alcohol (95% ethanol in 12 ounces of orange juice) per day, in random order. During the controlled feeding periods, all food and beverages including alcoholic beverages were prepared and supplied by the staff of the Beltsville Human Nutrition Research Center's Human Study Facility (Beltsville, MD), and energy intake was adjusted to maintain a constant weight. Blood was collected after an overnight fast on 3 separate days during the last week of each controlled feeding period and pooled for hormone analysis. Circulating serum leptin concentration was measured in duplicate by RIA and genotype analysis was done on DNA extracted from WBC using real-time PCR analysis amplification (TaqMan). Linear mixed models with a single random intercept reflecting a participant effect were used to estimate changes in serum leptin levels at 15 and 30 g of alcohol per day relative to 0 g of alcohol per day. No significant effects were found between common leptin receptor polymorphisms and serum leptin levels (P greater than or equal to 0.26). JF - Cancer Epidemiology, Biomarkers & Prevention AU - Roth, Mark J AU - Paltoo, Dina N AU - Albert, Paul S AU - Baer, David J AU - Judd, Joseph T AU - Tangrea, Joseph AU - Taylor, Philip R AD - Nutritional Epidemiology Branch, Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, Biometric Research Branch, Division of Cancer Treatment and Diagnosis, Center for Cancer Research, National Cancer Institute, Clinical and Molecular Medicine Program, National Heart, Lung, and Blood Institute, Bethesda, Maryland Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1576 EP - 1578 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 6 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17355793?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Common+Leptin+Receptor+Polymorphisms+do+not+Modify+the+Effect+of+Alcohol+Ingestion+on+Serum+Leptin+Levels+in+a+Controlled+Feeding+and+Alcohol+Ingestion+Study&rft.au=Roth%2C+Mark+J%3BPaltoo%2C+Dina+N%3BAlbert%2C+Paul+S%3BBaer%2C+David+J%3BJudd%2C+Joseph+T%3BTangrea%2C+Joseph%3BTaylor%2C+Philip+R&rft.aulast=Roth&rft.aufirst=Mark&rft.date=2005-06-01&rft.volume=14&rft.issue=6&rft.spage=1576&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Crystal structure of the protease-resistant core domain of Yersinia pestis virulence factor YopR AN - 17353599; 6416782 AB - Yersinia pestis, the causative agent of the plague, employs a type III secretion system (T3SS) to secrete and translocate virulence factors into to the cytoplasm of mammalian host cells. One of the secreted virulence factors is YopR. Little is known about the function of YopR other than that it is secreted into the extracellular milieu during the early stages of infection and that it contributes to virulence. Hoping to gain some insight into the function of YopR, we determined the crystal structure of its protease-resistant core domain, which consists of residues 38-149 out of 165 amino acids. The core domain is composed of five alpha -helices that display unexpected structural similarity with one domain of YopN, a central regulator of type III secretion in Y. pestis. This finding raises the possibility that YopR may play a role in the regulation of type III secretion. JF - Protein Science AU - Schubot, Florian D AU - Cherry, Scott AU - Austin, Brian P AU - Tropea, Joseph E AU - Waugh, David S AD - Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702-1201, USA Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1679 EP - 1683 PB - Cold Spring Harbor Laboratory Press, [mailto:cshpress@cshl.org], [URL:http://www.cshl.org/] VL - 14 IS - 6 SN - 0961-8368, 0961-8368 KW - YopR protein KW - Microbiology Abstracts B: Bacteriology KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17353599?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Science&rft.atitle=Crystal+structure+of+the+protease-resistant+core+domain+of+Yersinia+pestis+virulence+factor+YopR&rft.au=Schubot%2C+Florian+D%3BCherry%2C+Scott%3BAustin%2C+Brian+P%3BTropea%2C+Joseph+E%3BWaugh%2C+David+S&rft.aulast=Schubot&rft.aufirst=Florian&rft.date=2005-06-01&rft.volume=14&rft.issue=6&rft.spage=1679&rft.isbn=&rft.btitle=&rft.title=Protein+Science&rft.issn=09618368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Somatic Stem Cell Marker Prominin-1/CD133 Is Expressed in Embryonic Stem Cell-Derived Progenitors AN - 17346812; 6416896 AB - Prominin-1/CD133 is a plasma membrane marker found in several types of somatic stem cells, including hematopoietic and neural stem cells. To study its role during development and with differentiation, we analyzed its temporal and spatial expression (mRNA and protein) in preimplantation embryos, undifferentiated mouse embryonic stem (ES) cells, and differentiated ES cell progeny. In early embryos, prominin-1 was expressed in trophoblast but not in cells of the inner cell mass; however, prominin-1 transcripts were detected in undifferentiated ES cells. Both ES-derived cells committed to differentiation and early progenitor cells coexpressed prominin-1 with early lineage markers, including the cytoskeletal markers (nestin, cytokeratin 18, desmin), fibulin-1, and valosin-containing protein. After spontaneous differentiation at terminal stages, prominin-1 expression was downregulated and no coexpression with markers characteristic for neuroectodermal, mesodermal, and endodermal cells was found. Upon induction of neuronal differentiation, some prominin-1-positive cells, which coexpressed nestin and showed the typical morphology of neural progenitor cells, persisted until terminal stages of differentiation. However, no coexpression of prominin-1 with markers of differentiated neural cells was detected. In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells. JF - Stem Cells AU - Kania, Gabriela AU - Corbeil, Denis AU - Fuchs, Joerg AU - Tarasov, Kirill V AU - Blyszczuk, Przemyslaw AU - Huttner, Wieland B AU - Boheler, Kenneth R AU - Wobus, Anna M AD - Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany. Medical Clinic and Polyclinic I, Carl Gustav Carus University, Dresden, Germany. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. Laboratory of Cardiovascular Science, National Institute on Aging, NIH, Baltimore, Maryland, USA Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 791 EP - 804 PB - AlphaMed Press, Inc., One Prestige Pl, Ste 290 Miamisburg OH 45342-3758 USA VL - 23 IS - 6 SN - 1066-5099, 1066-5099 KW - AC133 antigen KW - CD133 antigen KW - Prominin-1 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; CSA Neurosciences Abstracts KW - W3 33340:Other proteins, peptides, amino acids KW - N3 11044:Mammals (except primates) KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17346812?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Stem+Cells&rft.atitle=Somatic+Stem+Cell+Marker+Prominin-1%2FCD133+Is+Expressed+in+Embryonic+Stem+Cell-Derived+Progenitors&rft.au=Kania%2C+Gabriela%3BCorbeil%2C+Denis%3BFuchs%2C+Joerg%3BTarasov%2C+Kirill+V%3BBlyszczuk%2C+Przemyslaw%3BHuttner%2C+Wieland+B%3BBoheler%2C+Kenneth+R%3BWobus%2C+Anna+M&rft.aulast=Kania&rft.aufirst=Gabriela&rft.date=2005-06-01&rft.volume=23&rft.issue=6&rft.spage=791&rft.isbn=&rft.btitle=&rft.title=Stem+Cells&rft.issn=10665099&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Research on Environmental Health Interventions: Ethical Problems and Solutions AN - 17188879; 6858158 AB - This article reviews a variety of ethical issues one must consider when conducting research on environmental health interventions on human subjects. The paper uses the Kennedy Krieger Institute lead abatement study as well as a hypothetical asthma study to discuss questions concerning benefits and risks, risk minimization, safety monitoring, the duty to warn, the duty to report, the use of control groups, informed consent, equitable subject selection, privacy, conflicts of interest, and community consultation. Research on environmental health interventions can make an important contribution to our understanding of human health and disease prevention, provided it is conducted in a manner that meets prevailing scientific, ethical, and legal standards for research on human subjects. JF - Accountability in Research AU - Resnik, D B AU - Zeldin, D C AU - Sharp, R R AD - National Institute of Environmental Health Sciences, National Institutes of Health Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 69 EP - 101 VL - 12 IS - 2 SN - 0898-9621, 0898-9621 KW - Health & Safety Science Abstracts; Risk Abstracts KW - Asthma KW - Environmental health KW - accountability KW - Respiratory diseases KW - Lead KW - conflict of interests KW - risk reduction KW - Reviews KW - intervention KW - Legal aspects KW - Ethics KW - prevention KW - Research programs KW - R2 23090:Policy and planning KW - H 12000:Epidemiology and Public Health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17188879?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Accountability+in+Research&rft.atitle=Research+on+Environmental+Health+Interventions%3A+Ethical+Problems+and+Solutions&rft.au=Resnik%2C+D+B%3BZeldin%2C+D+C%3BSharp%2C+R+R&rft.aulast=Resnik&rft.aufirst=D&rft.date=2005-06-01&rft.volume=12&rft.issue=2&rft.spage=69&rft.isbn=&rft.btitle=&rft.title=Accountability+in+Research&rft.issn=08989621&rft_id=info:doi/10.1080%2F08989620590957157 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-06-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Environmental health; Asthma; Respiratory diseases; accountability; Lead; conflict of interests; risk reduction; Ethics; Legal aspects; intervention; Reviews; prevention; Research programs DO - http://dx.doi.org/10.1080/08989620590957157 ER - TY - JOUR T1 - Intermolecular complementation achieves high-specificity tumor targeting by anthrax toxin AN - 17076537; 6709175 AB - Anthrax toxin protective antigen (PrAg) forms a heptamer in which the binding site for lethal factor (LF) spans two adjacent monomers. This suggested that high cell-type specificity in tumor targeting could be obtained using monomers that generate functional LF-binding sites only through intermolecular complementation. We created PrAg mutants with mutations affecting different LF-binding subsites and containing either urokinase plasminogen activator (uPA) or matrix metalloproteinase (MMP) cleavage sites. Individually, these PrAg mutants had low toxicity as a result of impaired LF binding, but when administered together to uPA- and MMP-expressing tumor cells, they assembled into functional LF-binding heteroheptamers. The mixture of two complementing PrAg variants had greatly reduced toxicity in mice and was highly effective in the treatment of aggressive transplanted tumors of diverse origin. These results show that anthrax toxin, and by implication other multimeric toxins, offer excellent opportunities to introduce multiple-specificity determinants and thereby achieve high therapeutic indices. JF - Nature Biotechnology AU - Liu, Shihui AU - Redeye, Vivien AU - Kuremsky, Jeffrey G AU - Kuhnen, Marissa AU - Molinolo, Alfredo AU - Bugge, Thomas H AU - Leppla, Stephen H AD - Microbial Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA., thomas.bugge@nih.gov Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 725 EP - 730 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 23 IS - 6 SN - 1087-0156, 1087-0156 KW - Toxicology Abstracts; Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Lethal factor KW - protective antigen KW - Matrix metalloproteinase KW - Tumors KW - Toxicity KW - Tumor cells KW - Toxins KW - Monomers KW - Complementation KW - Anthrax KW - u-Plasminogen activator KW - Mutation KW - plasminogen KW - X 24370:Natural Toxins KW - W3 33374:Antitumor agents KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17076537?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Biotechnology&rft.atitle=Intermolecular+complementation+achieves+high-specificity+tumor+targeting+by+anthrax+toxin&rft.au=Liu%2C+Shihui%3BRedeye%2C+Vivien%3BKuremsky%2C+Jeffrey+G%3BKuhnen%2C+Marissa%3BMolinolo%2C+Alfredo%3BBugge%2C+Thomas+H%3BLeppla%2C+Stephen+H&rft.aulast=Liu&rft.aufirst=Shihui&rft.date=2005-06-01&rft.volume=23&rft.issue=6&rft.spage=725&rft.isbn=&rft.btitle=&rft.title=Nature+Biotechnology&rft.issn=10870156&rft_id=info:doi/10.1038%2Fnbt1091 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Lethal factor; protective antigen; Matrix metalloproteinase; Toxicity; Tumors; Tumor cells; Toxins; Monomers; Complementation; Anthrax; u-Plasminogen activator; Mutation; plasminogen DO - http://dx.doi.org/10.1038/nbt1091 ER - TY - JOUR T1 - Effects of salinity on copper accumulation in the common killifish (Fundulus heteroclitus) AN - 16209355; 6278928 AB - Results of laboratory and field studies have demonstrated that salinity influences the accumulation of copper. The present study is, to our knowledge, the first to examine the effect of salinity on copper accumulation in a teleost fish across a comprehensive range of salinity from freshwater to seawater. This was done in an effort to identify potential target tissues and differences in chemical interactions across salinities that will aid in the development of a seawater biotic ligand model (BLM) for copper. Killifish (Fundulus heteroclitus) were acclimated to five salinities (0, 5, 11, 22, and 28 ppt) and exposed to three copper concentrations (0 [nominal], 30, and 150 mu g L super(-1)), yielding 15 treatment groups. Fish from each group were sampled for tissue copper analysis at 0, 4, 12, and 30 d postexposure. Whole-body and liver accumulations were highest at lower salinities. The liver accounted for 57 to 86% of the whole-body copper even though it accounted for less than 4% of the body mass. Similarly, the gill accumulated more copper at lower salinities, whereas the intestine generally accumulated more copper at higher salinities. Speciation calculations indicate that CuCO sub(3) likely accounts for much of the accumulation, possibly with some contributions from CuOH super(+) and Cu(OH) sub(2). The free ion, Cu super(2+), does not appear to be associated with copper accumulation. However, the differences in physiology and in the concentrations of competing cations across salinities suggest that speciation alone cannot explain accumulation. The present findings may have implications for future development of a BLM for saline environments by identifying potential target tissues. JF - Environmental Toxicology and Chemistry AU - Blanchard, J AU - Grosell, M AD - Rosenstiel School of Marine and Atmospheric Science, Division of Marine Biology and Fisheries, NIEHS Marine and Freshwater Biomedical Sciences Center, University of Miami, 4600 Rickenbacker Causeway, Miami, Florida 33149, USA, jblanchard@rsmas.miami.edu Y1 - 2005/06// PY - 2005 DA - Jun 2005 SP - 1403 EP - 1413 VL - 24 IS - 6 SN - 0730-7268, 0730-7268 KW - Mummichog KW - ASFA 3: Aquatic Pollution & Environmental Quality; Toxicology Abstracts; Aqualine Abstracts; Water Resources Abstracts; Pollution Abstracts KW - Saline environments KW - Tissues KW - Physiology KW - Chemical Analysis KW - Copper KW - Freshwater KW - Marine fish KW - Salinity KW - Marine environment KW - Gills KW - Laboratory Equipment KW - Freshwater pollution KW - Fundulus heteroclitus KW - Freshwater environments KW - Laboratories KW - Brackish KW - Model Studies KW - Cations KW - Fish physiology KW - Intestine KW - Fish KW - Chemical interactions KW - Speciation KW - Water Analysis KW - Seawater KW - Body mass KW - Environmental factors KW - Killifish KW - Salinity effects KW - Marine KW - Toxicity KW - Bioaccumulation KW - Liver KW - Ligands KW - Q5 08504:Effects on organisms KW - AQ 00008:Effects of Pollution KW - SW 3030:Effects of pollution KW - P 6000:TOXICOLOGY AND HEALTH KW - X 24163:Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/16209355?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aaqualine&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Toxicology+and+Chemistry&rft.atitle=Effects+of+salinity+on+copper+accumulation+in+the+common+killifish+%28Fundulus+heteroclitus%29&rft.au=Blanchard%2C+J%3BGrosell%2C+M&rft.aulast=Blanchard&rft.aufirst=J&rft.date=2005-06-01&rft.volume=24&rft.issue=6&rft.spage=1403&rft.isbn=&rft.btitle=&rft.title=Environmental+Toxicology+and+Chemistry&rft.issn=07307268&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2014-05-07 N1 - SubjectsTermNotLitGenreText - Marine fish; Bioaccumulation; Fish physiology; Salinity effects; Copper; Environmental factors; Gills; Ligands; Saline environments; Cations; Freshwater environments; Marine environment; Body mass; Intestine; Liver; Tissues; Salinity; Seawater; Physiology; Chemical interactions; Toxicity; Freshwater pollution; Speciation; Water Analysis; Laboratories; Chemical Analysis; Model Studies; Killifish; Fish; Laboratory Equipment; Fundulus heteroclitus; Freshwater; Brackish; Marine ER - TY - JOUR T1 - Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans. AN - 67892126; 15905333 AB - The pathways for differentiation of human CD4(+) T cells into functionally distinct subsets of memory cells in vivo are unknown. The identification of these subsets and pathways has clear implications for the design of vaccines and immune-targeted therapies. Here, we show that populations of apparently naive CD4(+) T cells express the chemokine receptors CXCR3 or CCR4 and demonstrate patterns of gene expression and functional responses characteristic of memory cells. The proliferation history and T cell receptor repertoire of these chemokine-receptor(+) cells suggest that they are very early memory CD4(+) T cells that have "rested down" before acquiring the phenotypes described for "central" or "effector" memory T cells. In addition, the chemokine-receptor(+) "naive" populations contain Th1 and Th2 cells, respectively, demonstrating that Th1/Th2 differentiation can occur very early in vivo in the absence of markers conventionally associated with memory cells. We localized ligands for CXCR3 and CCR4 to separate foci in T cell zones of tonsil, suggesting that the chemokine-receptor(+) subsets may be recruited and contribute to segregated, polarized microenvironments within lymphoid organs. Importantly, our data suggest that CD4(+) T cells do not differentiate according to a simple schema from naive --> CD45RO(+) noneffector/central memory --> effector/effector memory cells. Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Song, Kaimei AU - Rabin, Ronald L AU - Hill, Brenna J AU - De Rosa, Stephen C AU - Perfetto, Stephen P AU - Zhang, Hongwei H AU - Foley, John F AU - Reiner, Jeffrey S AU - Liu, Jie AU - Mattapallil, Joseph J AU - Douek, Daniel C AU - Roederer, Mario AU - Farber, Joshua M AD - Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/31/ PY - 2005 DA - 2005 May 31 SP - 7916 EP - 7921 VL - 102 IS - 22 SN - 0027-8424, 0027-8424 KW - CCR4 protein, human KW - 0 KW - CXCR3 protein, human KW - Ligands KW - Receptors, CCR4 KW - Receptors, CXCR3 KW - Receptors, Chemokine KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - In Situ Hybridization KW - Palatine Tonsil -- cytology KW - Cells, Cultured KW - Humans KW - Receptors, Chemokine -- metabolism KW - Flow Cytometry KW - Reverse Transcriptase Polymerase Chain Reaction KW - T-Lymphocyte Subsets -- cytology KW - CD4-Positive T-Lymphocytes -- cytology KW - Gene Expression -- immunology KW - CD4-Positive T-Lymphocytes -- metabolism KW - Immunologic Memory -- immunology KW - Cell Differentiation -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67892126?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Characterization+of+subsets+of+CD4%2B+memory+T+cells+reveals+early+branched+pathways+of+T+cell+differentiation+in+humans.&rft.au=Song%2C+Kaimei%3BRabin%2C+Ronald+L%3BHill%2C+Brenna+J%3BDe+Rosa%2C+Stephen+C%3BPerfetto%2C+Stephen+P%3BZhang%2C+Hongwei+H%3BFoley%2C+John+F%3BReiner%2C+Jeffrey+S%3BLiu%2C+Jie%3BMattapallil%2C+Joseph+J%3BDouek%2C+Daniel+C%3BRoederer%2C+Mario%3BFarber%2C+Joshua+M&rft.aulast=Song&rft.aufirst=Kaimei&rft.date=2005-05-31&rft.volume=102&rft.issue=22&rft.spage=7916&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-06-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nature. 1999 Oct 14;401(6754):708-12 [10537110] Nat Med. 2001 Feb;7(2):245-8 [11175858] Lancet. 2000 May 27;355(9218):1875-81 [10866444] J Leukoc Biol. 2000 Oct;68(4):568-74 [11037980] Immunity. 2001 Mar;14(3):205-15 [11290331] J Exp Med. 2001 Apr 16;193(8):987-93 [11304560] J Immunol Methods. 2002 Jan 1;259(1-2):55-64 [11730841] J Exp Med. 2002 Mar 18;195(6):789-94 [11901204] Nature. 2002 May 2;417(6884):95-8 [11986671] Nat Rev Immunol. 2002 Apr;2(4):251-62 [12001996] Eur J Immunol. 2002 May;32(5):1264-73 [11981813] Nat Rev Immunol. 2002 Dec;2(12):933-44 [12461566] J Immunol. 2003 Jan 1;170(1):28-32 [12496379] Nat Immunol. 2003 Mar;4(3):225-34 [12563257] Nat Immunol. 2003 Sep;4(9):835-42 [12942084] J Immunol. 2003 Sep 15;171(6):2812-24 [12960302] Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3874-9 [15001705] Annu Rev Immunol. 2004;22:745-63 [15032595] J Exp Med. 2004 Sep 20;200(6):725-35 [15381728] J Immunol. 1993 Feb 1;150(3):1105-21 [7678616] Cytometry. 1995 Oct 1;21(2):187-96 [8582239] J Immunol. 1999 Apr 1;162(7):3840-50 [10201901] Science. 1999 Apr 30;284(5415):819-22 [10221917] Scand J Immunol. 1999 Jun;49(6):649-54 [10354377] Int Immunol. 1999 Nov;11(11):1801-10 [10545484] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Dissecting the Genetic Basis of Cancer Through the Study of Common Genetic Variation T2 - VIII International Symposium on Mutations in the Genome (Mutation Detection 2005) AN - 39684876; 3955857 JF - VIII International Symposium on Mutations in the Genome (Mutation Detection 2005) AU - Chanock, Stephen Y1 - 2005/05/31/ PY - 2005 DA - 2005 May 31 KW - Genetic diversity KW - Cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39684876?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=VIII+International+Symposium+on+Mutations+in+the+Genome+%28Mutation+Detection+2005%29&rft.atitle=Dissecting+the+Genetic+Basis+of+Cancer+Through+the+Study+of+Common+Genetic+Variation&rft.au=Chanock%2C+Stephen&rft.aulast=Chanock&rft.aufirst=Stephen&rft.date=2005-05-31&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=VIII+International+Symposium+on+Mutations+in+the+Genome+%28Mutation+Detection+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://www.genomic.unimelb.edu.au/program.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Lymphocyte toxicity and T cell receptor excision circles in workers exposed to benzene. AN - 67902522; 15935806 AB - We have previously reported that benzene decreases peripheral white blood cell and platelet counts and specifically lowers subsets of several blood cell types, including CD4+-T cells, B cells, NK cells, and granulocytes. Diminished thymus function has been implicated as a mechanism for CD4+-T cell loss in other conditions such as AIDS by assays of T cell receptor excision circles (TRECs), a marker of naive T cells that have recently emigrated from the thymus. To evaluate alteration of thymic function as a mechanism for benzene's effects on CD4+-T cell counts, we measured total TREC levels in 45 benzene-exposed workers and 45 unexposed controls. There was no significant difference in TREC levels per 10(6) peripheral blood leukocytes in the benzene-exposed workers compared to the controls. Although our study does not rule out counterbalancing alterations of TREC levels in specific T cell subsets, benzene's lymphotoxicity does not appear to be mediated through diminished thymus function. JF - Chemico-biological interactions AU - Lan, Qing AU - Zhang, Luoping AU - Hakim, Fran AU - Shen, Min AU - Memon, Sarfraz AU - Li, Guilan AU - Vermeulen, Roel AU - Smith, Martyn T AU - Rappaport, Stephen M AU - Hayes, Richard AU - Linet, Martha AU - Yin, Songnian AU - Rothman, Nathaniel AU - Rabkin, Charles S AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, 6120 Executive Blvd., EPS 8109, Bethesda, MD 20892-7240, USA. qingl@mail.nih.gov Y1 - 2005/05/30/ PY - 2005 DA - 2005 May 30 SP - 111 EP - 115 VL - 153-154 SN - 0009-2797, 0009-2797 KW - Air Pollutants, Occupational KW - 0 KW - DNA, Circular KW - Receptors, Antigen, T-Cell KW - Benzene KW - J64922108F KW - Index Medicus KW - Leukocytes -- immunology KW - Humans KW - CD4-Positive T-Lymphocytes -- immunology KW - CD4-Positive T-Lymphocytes -- drug effects KW - Thymus Gland -- drug effects KW - Blood Cell Count KW - Environmental Monitoring KW - Thymus Gland -- immunology KW - Adult KW - Recombination, Genetic KW - Shoes KW - China KW - Female KW - Male KW - Occupational Exposure KW - Benzene -- analysis KW - Air Pollutants, Occupational -- analysis KW - DNA, Circular -- blood KW - Benzene -- toxicity KW - Receptors, Antigen, T-Cell -- immunology KW - Air Pollutants, Occupational -- toxicity KW - DNA, Circular -- analysis KW - Receptors, Antigen, T-Cell -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67902522?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Lymphocyte+toxicity+and+T+cell+receptor+excision+circles+in+workers+exposed+to+benzene.&rft.au=Lan%2C+Qing%3BZhang%2C+Luoping%3BHakim%2C+Fran%3BShen%2C+Min%3BMemon%2C+Sarfraz%3BLi%2C+Guilan%3BVermeulen%2C+Roel%3BSmith%2C+Martyn+T%3BRappaport%2C+Stephen+M%3BHayes%2C+Richard%3BLinet%2C+Martha%3BYin%2C+Songnian%3BRothman%2C+Nathaniel%3BRabkin%2C+Charles+S&rft.aulast=Lan&rft.aufirst=Qing&rft.date=2005-05-30&rft.volume=153-154&rft.issue=&rft.spage=111&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-03 N1 - Date created - 2005-06-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in the pathogenesis of hepatocellular carcinoma. AN - 67871591; 15918183 AB - DNA mismatch repair (MMR) is an important mechanism for maintaining fidelity of genomic DNA. Abnormalities in one or more MMR genes are implicated in the development of many cancers. We investigated the role of expression of MMR genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in hepatocellular carcinogenesis. We evaluated the expression level of MMR genes in 33 hepatocellular carcinoma (HCC) cases using the multiplex reverse transcription (RT) PCR assays, as well as in 16 cases of normal adjacent hepatic tissues. beta-actin gene was used as an internal control and calibrator for quantification of gene expression. Out of the 33 studied cases, 25 were HCV positive and 30 (90.9%) showed reduced expression in one or more of the studied MMR genes. Reduced expression was found in hMSH2 (71.9%), hMLH1 (53.3%), GTBP (51.1%), hPMS2 (33.3%) and hPMS1 (6%). A significant correlation was found between reduced expression of hPMS2 (P = 0.0069) and GTBP (P = 0.0034), hPMS2 and non-cirrhosis (P = 0.0197), hMLH1 and high grade. On the other hand, 57.1%, 50%, 20%, 18.8%, and 6% of the normal tissues distant to tumors showed reduced expression of hMSH2, hMLH1, GTBP, hPMS2, and hPMS1 respectively. Multivariate analysis revealed a significant correlation between the expression level of hMSH2 (P = 0.008), hMLH1 (P = 0.001) and GTBP (P = 0.032) and HCC, between hPMS2, GTBP and HCV-associated HCC (P<0.001, 0.002). Reduced expression of MMR genes seems to play an important role in HCV-associated HCC. hPMS2 is likely involved at an early stage of hepatocarcinogenesis since it was detected in normal adjacent tissues. Reduced expression of hPMS2 provides a growth advantage and stimulates proliferation which encourages malignant transformation in non-cirrhotic HCV-infected patients via acquisition of more genetic damages. JF - World journal of gastroenterology AU - Zekri, Abdel-Rahman N AU - Sabry, Gelane M AU - Bahnassy, Abeer A AU - Shalaby, Kamal A AU - Abdel-Wahabh, Sabrin A AU - Zakaria, Serag AD - Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Kasr El-Aini St., Fom El-Khaig, Cairo 11796, Egypt. ncizakri@starnet.com.eg Y1 - 2005/05/28/ PY - 2005 DA - 2005 May 28 SP - 3020 EP - 3026 VL - 11 IS - 20 SN - 1007-9327, 1007-9327 KW - Index Medicus KW - Hepacivirus -- physiology KW - Humans KW - Middle Aged KW - Liver Cirrhosis -- genetics KW - Male KW - Female KW - DNA Repair -- genetics KW - Carcinoma, Hepatocellular -- virology KW - Liver Neoplasms -- pathology KW - Carcinoma, Hepatocellular -- genetics KW - Liver Neoplasms -- virology KW - Carcinoma, Hepatocellular -- pathology KW - Base Pair Mismatch -- genetics KW - Liver Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67871591?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=World+journal+of+gastroenterology&rft.atitle=Mismatch+repair+genes+%28hMLH1%2C+hPMS1%2C+hPMS2%2C+GTBP%2FhMSH6%2C+hMSH2%29+in+the+pathogenesis+of+hepatocellular+carcinoma.&rft.au=Zekri%2C+Abdel-Rahman+N%3BSabry%2C+Gelane+M%3BBahnassy%2C+Abeer+A%3BShalaby%2C+Kamal+A%3BAbdel-Wahabh%2C+Sabrin+A%3BZakaria%2C+Serag&rft.aulast=Zekri&rft.aufirst=Abdel-Rahman&rft.date=2005-05-28&rft.volume=11&rft.issue=20&rft.spage=3020&rft.isbn=&rft.btitle=&rft.title=Elementary+School+Journal&rft.issn=00135984&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Race against time. AN - 67869088; 15917781 JF - Nature AU - Fauci, Anthony S AD - National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-2520, USA. Y1 - 2005/05/26/ PY - 2005 DA - 2005 May 26 SP - 423 EP - 424 VL - 435 IS - 7041 KW - Antiviral Agents KW - 0 KW - Influenza Vaccines KW - Index Medicus KW - United States KW - Antiviral Agents -- therapeutic use KW - Animals KW - Biomedical Research -- trends KW - Humans KW - National Institutes of Health (U.S.) -- economics KW - Clinical Trials as Topic KW - Influenza Vaccines -- immunology KW - Influenza Vaccines -- economics KW - Population Surveillance KW - Genotype KW - Antiviral Agents -- supply & distribution KW - International Cooperation KW - Adult KW - Birds -- virology KW - Influenza Vaccines -- supply & distribution KW - Middle Aged KW - Antiviral Agents -- adverse effects KW - Adolescent KW - Time Factors KW - Orthomyxoviridae -- pathogenicity KW - Orthomyxoviridae -- genetics KW - Influenza, Human -- prevention & control KW - Influenza, Human -- epidemiology KW - Disaster Planning -- trends KW - Influenza, Human -- virology KW - Influenza, Human -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67869088?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Race+against+time.&rft.au=Fauci%2C+Anthony+S&rft.aulast=Fauci&rft.aufirst=Anthony&rft.date=2005-05-26&rft.volume=435&rft.issue=7041&rft.spage=423&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Uptake and neuritic transport of scrapie prion protein coincident with infection of neuronal cells. AN - 67874581; 15917460 AB - Invasion of the nervous system and neuronal spread of infection are critical, but poorly understood, steps in the pathogenesis of transmissible spongiform encephalopathies or prion diseases. To characterize pathways for the uptake and intraneuronal trafficking of infectious, protease-resistant prion protein (PrP-res), fluorescent-labeled PrP-res was used to infect a neuronally derived murine cell line (SN56) and adult hamster cortical neurons in primary culture. Concurrent with the establishment of persistent scrapie infection, SN56 cells internalized PrP-res aggregates into vesicles positive for markers for late endosomes and/or lysosomes but not synaptic, early endocytic, or raft-derived vesicles. Internalized PrP-res was then transported along neurites to points of contact with other cells. Similar trafficking was observed with dextran, Alzheimer's Abeta1-42 fibrils and noninfectious recombinant PrP fibrils, suggesting that PrP-res is internalized by a relatively nonspecific pinocytosis or transcytosis mechanism. Hamster cortical neurons were also capable of internalizing and disseminating exogenous PrP-res. Similar trafficking of exogenous PrP-res by cortical neurons cultured from the brains of PrP knock-out mice showed that uptake and neuritic transport did not require the presence of endogenous cellular PrP. These experiments visualize and characterize the initial steps associated with prion infection and transport within neuronal cells. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Magalhães, Ana Cristina AU - Baron, Gerald S AU - Lee, Kil Sun AU - Steele-Mortimer, Olivia AU - Dorward, David AU - Prado, Marco A M AU - Caughey, Byron AD - Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 SP - 5207 EP - 5216 VL - 25 IS - 21 KW - PrPSc Proteins KW - 0 KW - Green Fluorescent Proteins KW - 147336-22-9 KW - rab7 protein KW - 152989-05-4 KW - Cholera Toxin KW - 9012-63-9 KW - Endopeptidase K KW - EC 3.4.21.64 KW - rab GTP-Binding Proteins KW - EC 3.6.5.2 KW - Index Medicus KW - Animals KW - Cerebral Cortex -- cytology KW - Endopeptidase K -- pharmacology KW - Fluorescent Antibody Technique -- methods KW - Biological Transport KW - Mutagenesis -- physiology KW - Blotting, Western -- methods KW - Mice KW - rab GTP-Binding Proteins -- genetics KW - Diagnostic Imaging -- methods KW - Transfection -- methods KW - Cells, Cultured KW - rab GTP-Binding Proteins -- metabolism KW - Time Factors KW - Green Fluorescent Proteins -- metabolism KW - Protein Transport -- physiology KW - Cholera Toxin -- metabolism KW - Cricetinae KW - Neurons -- metabolism KW - Neurites -- virology KW - Nervous System Diseases -- metabolism KW - PrPSc Proteins -- metabolism KW - Neurons -- virology KW - Neurites -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67874581?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Uptake+and+neuritic+transport+of+scrapie+prion+protein+coincident+with+infection+of+neuronal+cells.&rft.au=Magalh%C3%A3es%2C+Ana+Cristina%3BBaron%2C+Gerald+S%3BLee%2C+Kil+Sun%3BSteele-Mortimer%2C+Olivia%3BDorward%2C+David%3BPrado%2C+Marco+A+M%3BCaughey%2C+Byron&rft.aulast=Magalh%C3%A3es&rft.aufirst=Ana&rft.date=2005-05-25&rft.volume=25&rft.issue=21&rft.spage=5207&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-21 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Human genetic disorders of lymphocyte homeostasis AN - 40068262; 3938287 AU - Lenardo, MJ Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40068262?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Human+genetic+disorders+of+lymphocyte+homeostasis&rft.au=Lenardo%2C+MJ&rft.aulast=Lenardo&rft.aufirst=MJ&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: Keystone Symposia, 221 Summit Place #272, Drawer 1630, Silverthorne, CO 80498; phone: 970-262-1230; fax: 970-262-1525; email: info@keystonesymposia.org; URL: www.keystonesymposia.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Pulmonary function abnormalities in children with osteogenesis imperfecta correlate with OI type and location of collagen mutation AN - 40052544; 3939927 AU - Flor-Cisneros, A AU - Chaney, H AU - Vojtova, J AU - Marini, J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40052544?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Pulmonary+function+abnormalities+in+children+with+osteogenesis+imperfecta+correlate+with+OI+type+and+location+of+collagen+mutation&rft.au=Flor-Cisneros%2C+A%3BChaney%2C+H%3BVojtova%2C+J%3BMarini%2C+J&rft.aulast=Flor-Cisneros&rft.aufirst=A&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American College of Medical Genetics, 9650 Rockville Pike, Bethesda, MD 20814, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Role of cytochrome P450 2E1 (CYP2E1) in trichloroethylene (TCE) metabolism and disposition: Comparative studies using CYP2E1-/- and wild-type mice AN - 40037524; 3930366 AU - Kim, D AU - Ghanayem, B I Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40037524?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Role+of+cytochrome+P450+2E1+%28CYP2E1%29+in+trichloroethylene+%28TCE%29+metabolism+and+disposition%3A+Comparative+studies+using+CYP2E1-%2F-+and+wild-type+mice&rft.au=Kim%2C+D%3BGhanayem%2C+B+I&rft.aulast=Kim&rft.aufirst=D&rft.date=2005-05-25&rft.volume=27&rft.issue=6&rft.spage=788&rft.isbn=&rft.btitle=&rft.title=Early+Education+and+Development&rft.issn=10409289&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - 14-week comparative study of 1, 2, 3, 4, 6, 7-hexachloronaphthalene (PCN 66) and 1, 2, 3, 5, 6, 7-hexachloronaphthalene (PCN 67) in female harlan Sprague-Dawley (HSD) and Fischer 344 (F344) rats AN - 39994287; 3931708 AU - Hooth, M AU - Vallant, M AU - Walker, N AU - Nyska, A AU - Toyoshiba, H AU - Chhabra, R AU - Ryan, M AU - Vasconcelos, D AU - Hejtmancik, M AU - Fomby, L Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39994287?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C-dependent+regulation+of+NAG-1%2Fplacental+bone+morphogenic+protein%2FMIC-1+expression+in+LNCaP+prostate+carcinoma+cells.&rft.au=Shim%2C+Minsub%3BEling%2C+Thomas+E&rft.aulast=Shim&rft.aufirst=Minsub&rft.date=2005-05-13&rft.volume=280&rft.issue=19&rft.spage=18636&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Neonatal exposure to the phytoestrogen genistein alters reproduction in female CD-1 mice AN - 39906708; 3931008 AU - Jefferson, W AU - Padilla-Bank, E AU - Newbold, R Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39906708?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Neonatal+exposure+to+the+phytoestrogen+genistein+alters+reproduction+in+female+CD-1+mice&rft.au=Jefferson%2C+W%3BPadilla-Bank%2C+E%3BNewbold%2C+R&rft.aulast=Jefferson&rft.aufirst=W&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Protocol optimization for the evaluation of in vitro cytotoxicity assays for estimating rodent and human acute systemic toxicity AN - 39901241; 3931266 AU - Paris, M AU - Strickland, J AU - Stokes, W AU - Casati, S AU - Tice, R AU - Raabe, H AU - Cao, C AU - Clothier, R AU - Haseman, J AU - Crockett, P Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39901241?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Protocol+optimization+for+the+evaluation+of+in+vitro+cytotoxicity+assays+for+estimating+rodent+and+human+acute+systemic+toxicity&rft.au=Paris%2C+M%3BStrickland%2C+J%3BStokes%2C+W%3BCasati%2C+S%3BTice%2C+R%3BRaabe%2C+H%3BCao%2C+C%3BClothier%2C+R%3BHaseman%2C+J%3BCrockett%2C+P&rft.aulast=Paris&rft.aufirst=M&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Testing the toxic equivalency factor (TEF) hypothesis: The NTP dioxin/PCB cancer bioassays AN - 39896646; 3929671 AU - Walker, N J AU - Wyde, ME AU - Crockett, P W AU - Nyska, A AU - Bucher, J R AU - Portier, C J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39896646?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Testing+the+toxic+equivalency+factor+%28TEF%29+hypothesis%3A+The+NTP+dioxin%2FPCB+cancer+bioassays&rft.au=Walker%2C+N+J%3BWyde%2C+ME%3BCrockett%2C+P+W%3BNyska%2C+A%3BBucher%2C+J+R%3BPortier%2C+C+J&rft.aulast=Walker&rft.aufirst=N&rft.date=2005-05-25&rft.volume=51&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Maturitas&rft.issn=03785122&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Proteomic analysis of whole liver and subcellular fractions following acetaminophen-induced hepatotoxicity in male rats AN - 39895127; 3929702 AU - Bruno, ME AU - Wetmore, BA AU - Madenspacher, J H AU - Pieper, R AU - McGrath, A M AU - Gatlin, CL AU - Makusky, A J AU - Zhao, M AU - Zhou, J AU - Taylor, J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39895127?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Proteomic+analysis+of+whole+liver+and+subcellular+fractions+following+acetaminophen-induced+hepatotoxicity+in+male+rats&rft.au=Bruno%2C+ME%3BWetmore%2C+BA%3BMadenspacher%2C+J+H%3BPieper%2C+R%3BMcGrath%2C+A+M%3BGatlin%2C+CL%3BMakusky%2C+A+J%3BZhao%2C+M%3BZhou%2C+J%3BTaylor%2C+J&rft.aulast=Bruno&rft.aufirst=ME&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - A Novel Algorithm for Microarray Time Series Data Analysis T2 - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AN - 39652265; 3949328 JF - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AU - McCall, Matthew N AU - Chen, Yidong AU - Davis, Sean AU - Walker, Robert L AU - Meltzer, Paul S Y1 - 2005/05/22/ PY - 2005 DA - 2005 May 22 KW - Algorithms KW - Data processing KW - U 5500:Geoscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39652265?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.atitle=A+Novel+Algorithm+for+Microarray+Time+Series+Data+Analysis&rft.au=McCall%2C+Matthew+N%3BChen%2C+Yidong%3BDavis%2C+Sean%3BWalker%2C+Robert+L%3BMeltzer%2C+Paul+S&rft.aulast=McCall&rft.aufirst=Matthew&rft.date=2005-05-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://dsplab.eng.umd.edu/gensips/program.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Using Genomic Data to Find Biological Mechanisms in Cancer: Beyond Expression Profiling T2 - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AN - 39647453; 3949281 JF - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AU - Meltzer, Paul Y1 - 2005/05/22/ PY - 2005 DA - 2005 May 22 KW - Profiling KW - Cancer KW - Genomics KW - U 5500:Geoscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39647453?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.atitle=Using+Genomic+Data+to+Find+Biological+Mechanisms+in+Cancer%3A+Beyond+Expression+Profiling&rft.au=Meltzer%2C+Paul&rft.aulast=Meltzer&rft.aufirst=Paul&rft.date=2005-05-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://dsplab.eng.umd.edu/gensips/program.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability. AN - 67949165; 15907206 AB - Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The effective correlation of observed changes in gene expression with shared transcription regulatory elements remains difficult to demonstrate convincingly. One reason for this difficulty may result from the intricate convergence of both transcriptional and mRNA turnover events which, together, directly influence steady-state mRNA levels. In order to investigate the relative contribution of gene transcription and changes in mRNA stability regulation to standard analyses of gene expression, we used two distinct microarray methods which individually measure nuclear gene transcription and changes in polyA mRNA gene expression. Gene expression profiles were obtained from both polyA mRNA (whole-cell) and nuclear run-on (newly transcribed) RNA across a time course of one hour following the activation of human Jurkat T cells with PMA plus ionomycin. Comparative analysis revealed that regulation of mRNA stability may account for as much as 50% of all measurements of changes in polyA mRNA in this system, as inferred by the absence of any corresponding regulation of nuclear gene transcription activity for these groups of genes. Genes which displayed dramatic elevations in both mRNA and nuclear run-on RNA were shown to be inhibited by Actinomycin D (ActD) pre-treatment of cells while large numbers of genes regulated only through altered mRNA turnover (both up and down) were ActD-resistant. Consistent patterns across the time course were observed for both transcribed and stability-regulated genes. We propose that regulation of mRNA stability contributes significantly to the observed changes in gene expression in response to external stimuli, as measured by high throughput systems. JF - BMC genomics AU - Cheadle, Chris AU - Fan, Jinshui AU - Cho-Chung, Yoon S AU - Werner, Thomas AU - Ray, Jill AU - Do, Lana AU - Gorospe, Myriam AU - Becker, Kevin G AD - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA. cheadlec@mail.nih.gov Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 75 VL - 6 KW - NF-kappa B KW - 0 KW - RNA, Messenger KW - Dactinomycin KW - 1CC1JFE158 KW - Poly A KW - 24937-83-5 KW - Ionomycin KW - 56092-81-0 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Cell Nucleus -- metabolism KW - Humans KW - Computational Biology -- methods KW - Jurkat Cells KW - Ionomycin -- pharmacology KW - Poly A -- metabolism KW - Lymphocyte Activation KW - Dactinomycin -- pharmacology KW - Polymerase Chain Reaction KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Time Factors KW - Cluster Analysis KW - NF-kappa B -- metabolism KW - T-Lymphocytes -- metabolism KW - RNA, Messenger -- metabolism KW - Genomics -- methods KW - Transcription, Genetic KW - Gene Expression Regulation KW - Gene Expression Profiling -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67949165?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+genomics&rft.atitle=Control+of+gene+expression+during+T+cell+activation%3A+alternate+regulation+of+mRNA+transcription+and+mRNA+stability.&rft.au=Cheadle%2C+Chris%3BFan%2C+Jinshui%3BCho-Chung%2C+Yoon+S%3BWerner%2C+Thomas%3BRay%2C+Jill%3BDo%2C+Lana%3BGorospe%2C+Myriam%3BBecker%2C+Kevin+G&rft.aulast=Cheadle&rft.aufirst=Chris&rft.date=2005-05-20&rft.volume=6&rft.issue=&rft.spage=75&rft.isbn=&rft.btitle=&rft.title=BMC+genomics&rft.issn=1471-2164&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-01 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nucleic Acids Res. 1999 Dec 1;27(23):4609-18 [10556317] Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 [9843981] Nat Genet. 2000 May;25(1):96-101 [10802665] J Biol Chem. 2000 Aug 11;275(32):24375-82 [10825165] Nucleic Acids Res. 2001 Jan 15;29(2):397-406 [11139609] Nat Immunol. 2001 Apr;2(4):316-24 [11276202] Eur J Cell Biol. 2001 May;80(5):321-8 [11432721] J Biol Chem. 2002 Feb 8;277(6):4465-76 [11694517] Restor Neurol Neurosci. 2001;18(2-3):127-35 [11847435] Nat Genet. 2002 May;31(1):19-20 [11984561] J Biol Chem. 2002 Jun 21;277(25):22175-84 [11943782] Plant J. 2002 Jul;31(2):171-88 [12121447] Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10611-6 [12149460] Nucleic Acids Res. 2002 Dec 15;30(24):5529-38 [12490721] J Mol Diagn. 2003 May;5(2):73-81 [12707371] Mol Cell. 2004 Jul 23;15(2):303-13 [15260981] Brief Funct Genomic Proteomic. 2004 Aug;3(2):112-24 [15355594] Science. 1989 Jan 20;243(4889):355-61 [2783497] J Immunol. 1989 Sep 15;143(6):1790-4 [2506269] J Biol Chem. 1991 Jul 5;266(19):12157-61 [2061303] Science. 1995 Oct 20;270(5235):467-70 [7569999] Mol Immunol. 1995 Sep;32(13):947-55 [7477000] Biotechniques. 1996 Apr;20(4):584-91 [8800675] Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10614-9 [8855227] Mol Cell Biol. 1998 Mar;18(3):1400-7 [9488455] Curr Opin Immunol. 2000 Apr;12(2):206-9 [10712937] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase I study of hepatic arterial melphalan infusion and hepatic venous hemofiltration using percutaneously placed catheters in patients with unresectable hepatic malignancies. AN - 67854233; 15908655 AB - We conducted a phase I study of a 30-minute hepatic artery infusion of melphalan via a percutaneously placed catheter and hepatic venous hemofiltration using a double balloon catheter positioned in the retrohepatic inferior vena cava to shunt hepatic venous effluent through an activated charcoal filter and then to the systemic circulation. The purpose of the study was to demonstrate feasibility in an initial cohort and subsequently determine the maximum tolerated dose and dose-limiting toxicity of melphalan. The initial cohort (n = 12) was treated with 2.0 mg/kg of melphalan before dose escalation to 3.5 mg/kg (n = 16). Total hepatic drug delivery, systemic levels, and percent filter efficiency were determined. Patients were assessed for hepatic and systemic toxicity and response. A total of 74 treatments were administered to 28 patients. Twelve patients with primary and metastatic hepatic tumors received 30 treatments (mean, 2.5 per patient) at an initial melphalan dose of 2.0 mg/kg. At 3.5 mg/kg, a dose-limiting toxicity (neutropenia and/or thrombocytopenia) was observed in two of six patients. Transient grade 3/4 hepatic and systemic toxicity was seen after 19% and 66% of treatments, respectively. An overall radiographic response rate of 30% was observed in treated patients. In the 10 patients with ocular melanoma, a 50% overall response rate was observed, including two complete responses. Delivery of melphalan via this system is feasible, with limited, manageable toxicity and evidence of substantial antitumor activity; 3 mg/kg is the maximum safe tolerated dose of melphalan administered via this technique. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Pingpank, James F AU - Libutti, Steven K AU - Chang, Richard AU - Wood, Bradford J AU - Neeman, Ziv AU - Kam, Anthony W AU - Figg, William D AU - Zhai, Souping AU - Beresneva, Tatiana AU - Seidel, Geoffrey D AU - Alexander, H Richard AD - Surgical Metabolism Section, Surgery Branch, National Cancer Institute/National Institutes of Health, Bethesda, MD 20892-1502, USA. Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 3465 EP - 3474 VL - 23 IS - 15 SN - 0732-183X, 0732-183X KW - Melphalan KW - Q41OR9510P KW - Index Medicus KW - Probability KW - Drug Administration Schedule KW - Infusions, Intra-Arterial KW - Neoplasm Staging KW - Combined Modality Therapy KW - Dose-Response Relationship, Drug KW - Humans KW - Terminally Ill KW - Aged KW - Palliative Care -- methods KW - Risk Assessment KW - Catheterization KW - Adult KW - Treatment Outcome KW - Middle Aged KW - Follow-Up Studies KW - Maximum Tolerated Dose KW - Female KW - Male KW - Survival Analysis KW - Liver Neoplasms -- pathology KW - Liver Neoplasms -- therapy KW - Melphalan -- administration & dosage KW - Liver Neoplasms -- mortality KW - Hemofiltration -- methods KW - Chemotherapy, Cancer, Regional Perfusion -- methods KW - Liver Neoplasms -- secondary UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67854233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Phase+I+study+of+hepatic+arterial+melphalan+infusion+and+hepatic+venous+hemofiltration+using+percutaneously+placed+catheters+in+patients+with+unresectable+hepatic+malignancies.&rft.au=Pingpank%2C+James+F%3BLibutti%2C+Steven+K%3BChang%2C+Richard%3BWood%2C+Bradford+J%3BNeeman%2C+Ziv%3BKam%2C+Anthony+W%3BFigg%2C+William+D%3BZhai%2C+Souping%3BBeresneva%2C+Tatiana%3BSeidel%2C+Geoffrey+D%3BAlexander%2C+H+Richard&rft.aulast=Pingpank&rft.aufirst=James&rft.date=2005-05-20&rft.volume=23&rft.issue=15&rft.spage=3465&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-24 N1 - Date created - 2005-05-23 N1 - Date revised - 2017-01-14 N1 - SuppNotes - Cited By: J Clin Oncol. 2003 Jun 1;21(11):2059-69 [12775730] CA Cancer J Clin. 2003 Jan-Feb;53(1):5-26 [12568441] Surgery. 2001 Oct;130(4):677-82; discussion 682-5 [11602899] J Clin Oncol. 2001 May 15;19(10):2687-95 [11352961] J Am Coll Surg. 2000 Nov;191(5):519-30 [11085732] Oncologist. 2000;5(5):416-24 [11040278] Clin Cancer Res. 2000 Aug;6(8):3062-70 [10955785] Br J Cancer. 1982 Jan;45(1):17-26 [7059461] Cancer. 1983 Jul 15;52(2):334-6 [6190546] Surgery. 1986 Aug;100(2):285-91 [2943036] Surv Ophthalmol. 1988 Jan-Feb;32(4):239-51 [3279559] Ophthalmology. 1991 Mar;98(3):383-9; discussion 390 [2023760] Surgery. 1993 Sep;114(3):579-85 [8367814] Cancer. 1993 Oct 1;72(7):2219-23 [7848381] Cancer Chemother Pharmacol. 1993;33(3):251-7 [8269607] Int J Hyperthermia. 1994 Jan-Feb;10(1):89-99 [7511674] J Nucl Med. 1994 Oct;35(10):1637-44 [7931662] J Clin Oncol. 1994 Dec;12(12):2723-36 [7989950] Bone Marrow Transplant. 1994 Sep;14(3):443-8 [7994270] N Engl J Med. 1995 May 11;332(19):1256-61 [7708069] J Clin Oncol. 1995 Jul;13(7):1786-99 [7602368] J Clin Oncol. 1997 Jun;15(6):2420-31 [9196158] J Clin Oncol. 1998 Apr;16(4):1479-89 [9552055] Ann Surg. 1999 Jul;230(1):1-8 [10400029] Ann Surg. 1999 Sep;230(3):309-18; discussion 318-21 [10493478] N1 - Last updated - 2017-01-19 ER - TY - JOUR T1 - Investigating HIV-1 polypurine tract geometry via targeted insertion of abasic lesions in the (-)-DNA template and (+)-RNA primer. AN - 67829396; 15778225 AB - A variety of biochemical and structural studies indicate that two regions of the human immunodeficiency virus type 1 (HIV-1) polypurine tract (PPT)-containing RNA/DNA hybrid deviate from standard Watson-Crick geometry. However, it is unclear whether and how these regions cooperate to ensure PPT primer selection by reverse transcriptase-associated ribonuclease H and subsequent removal from nascent (+)-DNA. To address these issues, we synthesized oligonucleotides containing abasic lesions in either the PPT (+)-RNA primer or (-)-DNA template to locally remove nucleobases, although retaining the sugar-phosphate backbone. KMnO(4) footprinting indicates such lesions locally alter duplex structure, whereas thermal melting studies show significantly reduced stability when lesions are positioned around the scissile bond. Substituting the (-)-DNA template between positions -15 and -13 altered cleavage specificity, whereas equivalent substitutions of the (+)-RNA had almost no effect. The unpaired base of the DNA template observed crystallographically (-11C) could also be removed without significant loss of cleavage specificity. With respect to the scissile -1/+1 phosphodiester bond, template nucleobases could be removed without loss of cleavage specificity, whereas equivalent lesions in the RNA primer were inhibitory. Our data suggest an interaction between the p66 thumb subdomain of HIV-1 reverse transcriptase, and the DNA template in the "unzipped" portion of the RNA/DNA hybrid could aid in positioning the ribonuclease H catalytic center at the PPT/U3 junction and also provides insights into nucleic acid geometry around the scissile bond required for hydrolysis. JF - The Journal of biological chemistry AU - Yi-Brunozzi, Hye Young AU - Le Grice, Stuart F J AD - Reverse Transcriptase Biochemistry Section, Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI, National Institutes of Health, Frederick, Maryland 21702, USA. Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 20154 EP - 20162 VL - 280 IS - 20 SN - 0021-9258, 0021-9258 KW - DNA, Viral KW - 0 KW - Purines KW - RNA primers KW - RNA, Viral KW - RNA KW - 63231-63-0 KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - Index Medicus KW - Base Sequence KW - In Vitro Techniques KW - HIV Reverse Transcriptase -- metabolism KW - Reverse Transcription KW - Binding Sites -- genetics KW - Purines -- chemistry KW - Nucleic Acid Conformation KW - Models, Biological KW - Mutagenesis, Insertional KW - HIV-1 -- metabolism KW - HIV-1 -- genetics KW - HIV-1 -- chemistry KW - DNA, Viral -- chemistry KW - RNA, Viral -- chemistry KW - RNA, Viral -- genetics KW - RNA, Viral -- metabolism KW - DNA, Viral -- genetics KW - DNA, Viral -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829396?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Investigating+HIV-1+polypurine+tract+geometry+via+targeted+insertion+of+abasic+lesions+in+the+%28-%29-DNA+template+and+%28%2B%29-RNA+primer.&rft.au=Yi-Brunozzi%2C+Hye+Young%3BLe+Grice%2C+Stuart+F+J&rft.aulast=Yi-Brunozzi&rft.aufirst=Hye&rft.date=2005-05-20&rft.volume=280&rft.issue=20&rft.spage=20154&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The decrease in the presynaptic calcium current is a major cause of short-term depression at a calyx-type synapse. AN - 67915862; 15944131 AB - Repetitive nerve firings cause short-term depression (STD) of release at many synapses. Its underlying mechanism is largely attributed to depletion of a readily releasable vesicle pool (RRP) and a decreased probability of releasing a readily releasable vesicle during an action potential. Which of these two mechanisms is dominant and the mechanism that decreases the release probability remain debated. Here, we report that a decreased release probability is caused by a calcium-induced inhibition of presynaptic calcium channels, particularly P/Q-type channels at the calyx of Held in rat brainstem. This mechanism was the dominant cause of STD in a wide range of stimulation conditions, such as during 2 to 20 action potential-equivalent stimuli (AP-e) at 0.2-30 Hz and after 2 to 20 AP-e at 0.2-100 Hz. Only during > or = 100 Hz AP-e was depletion the dominant mechanism. JF - Neuron AU - Xu, Jianhua AU - Wu, Ling-Gang AD - National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA. Y1 - 2005/05/19/ PY - 2005 DA - 2005 May 19 SP - 633 EP - 645 VL - 46 IS - 4 SN - 0896-6273, 0896-6273 KW - Calcium Channels KW - 0 KW - Chelating Agents KW - Enzyme Inhibitors KW - Imidazoles KW - Nuclear Proteins KW - Peptides KW - Trans-Activators KW - MLCK peptide KW - 135467-90-2 KW - Barium KW - 24GP945V5T KW - calmidazolium KW - 4R9H38JAWL KW - Egtazic Acid KW - 526U7A2651 KW - CREB-Binding Protein KW - EC 2.3.1.48 KW - Crebbp protein, rat KW - 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid KW - K22DDW77C0 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Patch-Clamp Techniques -- methods KW - Electric Capacitance KW - Nuclear Proteins -- pharmacology KW - Action Potentials -- physiology KW - Animals KW - Electric Stimulation -- methods KW - Trans-Activators -- pharmacology KW - Imidazoles -- pharmacology KW - Action Potentials -- radiation effects KW - Action Potentials -- drug effects KW - Dose-Response Relationship, Radiation KW - Peptides -- pharmacology KW - Models, Biological KW - Rats KW - Animals, Newborn KW - Chelating Agents -- pharmacology KW - In Vitro Techniques KW - Rats, Wistar KW - Enzyme Inhibitors -- pharmacology KW - Brain Stem -- cytology KW - Time Factors KW - Barium -- pharmacology KW - Calcium -- metabolism KW - Presynaptic Terminals -- drug effects KW - Egtazic Acid -- analogs & derivatives KW - Calcium Channels -- radiation effects KW - Calcium Channels -- metabolism KW - Neural Inhibition -- radiation effects KW - Calcium Channels -- drug effects KW - Neural Inhibition -- drug effects KW - Synapses -- metabolism KW - Neural Inhibition -- physiology KW - Egtazic Acid -- pharmacology KW - Presynaptic Terminals -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67915862?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuron&rft.atitle=The+decrease+in+the+presynaptic+calcium+current+is+a+major+cause+of+short-term+depression+at+a+calyx-type+synapse.&rft.au=Xu%2C+Jianhua%3BWu%2C+Ling-Gang&rft.aulast=Xu&rft.aufirst=Jianhua&rft.date=2005-05-19&rft.volume=46&rft.issue=4&rft.spage=633&rft.isbn=&rft.btitle=&rft.title=Neuron&rft.issn=08966273&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-29 N1 - Date created - 2005-06-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Neuron. 2005 May 19;46(4):523-5 [15944119] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Coronary heart disease in menopausal women: implications of primary and secondary prevention trials of hormones. AN - 67809984; 15883110 AB - In direct contrast to the observational studies, both primary and secondary prevention trials of female reproductive hormones have found no benefit for coronary heart disease (CHD). Basic science studies have elucidated several mechanisms by which estrogen may improve coronary arterial physiology and prevent pathology, but have also found mechanisms by which estrogen might increase coagulation or inflammation, or might trigger coronary events in advanced lesions. Animal studies suggest that hormones may retard early atherosclerosis, while both animal studies and human angiographic trials are conclusive that hormones do not retard progression of raised lesions. Hormone use in the primary prevention observational studies would mostly have started at the age of menopause, in women whose arteries on average would be closer to normal than those of women in the clinical trials. One hypothesis worthy of further study is that estrogen may have a beneficial effect in normal or near-normal arteries, but the opposite effect in the presence of established atherosclerosis. However, at the average age of menopause, a substantial proportion of women has raised lesions, and a smaller proportion already has advanced lesions. Also, the apparent benefit of hormone use was found in secondary prevention observational studies, i.e., in women with compromised arteries. It is likely that uncorrected biases in the observational studies lead to an overestimation of any benefit of hormone use. On the other hand, endogenous estradiol may be responsible for the later onset of coronary disease in women compared to men; if so, then the appropriate test of the estrogen hypothesis would employ transdermal estradiol in a young population of menopausal women. Hormones are not indicated for the prevention of CHD, particularly in the light of the increased risk for stroke and venous thrombosis. Their use for other indications (menopausal symptoms, osteoporosis) needs to be tempered by the risk for cardiovascular disease (CVD). JF - Maturitas AU - Rossouw, Jacques E AD - National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-7966, USA. rossouwj@nih.gov Y1 - 2005/05/16/ PY - 2005 DA - 2005 May 16 SP - 51 EP - 63 VL - 51 IS - 1 SN - 0378-5122, 0378-5122 KW - Index Medicus KW - Blood Coagulation Disorders -- chemically induced KW - Animals KW - Randomized Controlled Trials as Topic KW - Angiography KW - Humans KW - Inflammation -- chemically induced KW - Aged KW - Middle Aged KW - Female KW - Atherosclerosis -- chemically induced KW - Primary Prevention KW - Hormone Replacement Therapy -- adverse effects KW - Coronary Disease -- prevention & control KW - Menopause -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67809984?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Maturitas&rft.atitle=Coronary+heart+disease+in+menopausal+women%3A+implications+of+primary+and+secondary+prevention+trials+of+hormones.&rft.au=Rossouw%2C+Jacques+E&rft.aulast=Rossouw&rft.aufirst=Jacques&rft.date=2005-05-16&rft.volume=51&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Maturitas&rft.issn=03785122&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-22 N1 - Date created - 2005-05-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibitors of the arachidonic acid pathway and peroxisome proliferator-activated receptor ligands have superadditive effects on lung cancer growth inhibition. AN - 67837647; 15899809 AB - Arachidonic acid (AA) metabolizing enzymes and peroxisome proliferator-activated receptors (PPARs) have been shown to regulate the growth of epithelial cells. We have previously reported that exposure to the 5-lipoxygenase activating protein-directed inhibitor MK886 but not the cyclooxygenase inhibitor, indomethacin, reduced growth, increased apoptosis, and up-regulated PPARalpha and gamma expression in breast cancer cell lines. In the present study, we explore approaches to maximizing the proapoptotic effects of PPARgamma on lung cancer cell lines. Non-small-cell cancer cell line A549 revealed dose-dependent PPARgamma reporter activity after treatment with MK886. The addition of indomethacin in combination with MK886 further increases reporter activity. We also show increased growth inhibition and up-regulation of apoptosis after exposure to MK886 alone, or in combination with indomethacin and the PPAR ligand, 15-deoxy-Delta12,14-prostaglandin J2 compared with single drug exposures on the adenocarcinoma cell line A549 and small-cell cancer cell lines H345, N417, and H510. Real-time PCR analyses showed increased PPAR mRNA and retinoid X receptor (RXR)alpha mRNA expression after exposure to MK886 and indomethacin in a time-dependent fashion. The results suggest that the principal proapoptotic effect of these drugs may be mediated through the known antiproliferative effects of the PPARgamma-RXR interaction. We therefore explored a three-drug approach to attempt to maximize this effect. The combination of low-dose MK886, ciglitazone, and 13-cis-retinoic acid interacted at least in a superadditive fashion to inhibit the growth of lung cancer cell lines A549 and H1299, suggesting that targeting PPARgamma and AA action is a promising approach to lung cancer growth with a favorable therapeutic index. JF - Cancer research AU - Avis, Ingalill AU - Martínez, Alfredo AU - Tauler, Jordi AU - Zudaire, Enrique AU - Mayburd, Anatoly AU - Abu-Ghazaleh, Raed AU - Ondrey, Frank AU - Mulshine, James L AD - Intervention Section, Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 4181 EP - 4190 VL - 65 IS - 10 SN - 0008-5472, 0008-5472 KW - Acetophenones KW - 0 KW - Indoles KW - Ligands KW - Peroxisome Proliferator-Activated Receptors KW - Pyrimidines KW - RNA, Messenger KW - Retinoid X Receptor alpha KW - Tetrazoles KW - Thiazolidinediones KW - MK-886 KW - 080626SQ8C KW - Arachidonic Acid KW - 27YG812J1I KW - 9-deoxy-delta-9-prostaglandin D2 KW - 60203-57-8 KW - pirinixic acid KW - 86C4MRT55A KW - LY 171883 KW - 88107-10-2 KW - Caspases KW - EC 3.4.22.- KW - Isotretinoin KW - EH28UP18IF KW - Prostaglandin D2 KW - RXY07S6CZ2 KW - ciglitazone KW - U8QXS1WU8G KW - Index Medicus KW - Retinoid X Receptor alpha -- biosynthesis KW - Cell Growth Processes -- physiology KW - Dose-Response Relationship, Drug KW - Retinoid X Receptor alpha -- genetics KW - Humans KW - Cell Line, Tumor KW - Tetrazoles -- administration & dosage KW - RNA, Messenger -- genetics KW - Pyrimidines -- administration & dosage KW - RNA, Messenger -- biosynthesis KW - Caspases -- metabolism KW - Isotretinoin -- administration & dosage KW - Acetophenones -- administration & dosage KW - Apoptosis -- drug effects KW - Enzyme Activation -- drug effects KW - Indoles -- administration & dosage KW - Thiazolidinediones -- administration & dosage KW - Arachidonic Acid -- antagonists & inhibitors KW - Peroxisome Proliferator-Activated Receptors -- biosynthesis KW - Prostaglandin D2 -- analogs & derivatives KW - Lung Neoplasms -- drug therapy KW - Peroxisome Proliferator-Activated Receptors -- metabolism KW - Antineoplastic Combined Chemotherapy Protocols -- pharmacology KW - Prostaglandin D2 -- administration & dosage KW - Peroxisome Proliferator-Activated Receptors -- genetics KW - Arachidonic Acid -- metabolism KW - Lung Neoplasms -- pathology KW - Lung Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67837647?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Inhibitors+of+the+arachidonic+acid+pathway+and+peroxisome+proliferator-activated+receptor+ligands+have+superadditive+effects+on+lung+cancer+growth+inhibition.&rft.au=Avis%2C+Ingalill%3BMart%C3%ADnez%2C+Alfredo%3BTauler%2C+Jordi%3BZudaire%2C+Enrique%3BMayburd%2C+Anatoly%3BAbu-Ghazaleh%2C+Raed%3BOndrey%2C+Frank%3BMulshine%2C+James+L&rft.aulast=Avis&rft.aufirst=Ingalill&rft.date=2005-05-15&rft.volume=65&rft.issue=10&rft.spage=4181&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-07 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Breast cancer cells induce stromal fibroblasts to express MMP-9 via secretion of TNF-alpha and TGF-beta. AN - 67830058; 15855236 AB - We used 2D-cocultures employing fibroblasts of different genetic backgrounds and MCF10A-derived human breast epithelial cells of increasingly malignant potential to investigate tumor-stroma interactions in breast cancer and to identify possible signaling pathways involved. Tumor cells induced expression of matrix-metalloproteinase 9 (MMP-9) in fibroblasts in a pattern dependent on the degree of their malignancy. In-situ zymography localized the main gelatinolytic activity around stromal cells in cocultures and xenografted tumors. Use of Smad3 knockout fibroblasts, small molecule inhibitors, and neutralizing antibodies showed that MMP-9 expression was induced by tumor cell-derived TNF-alpha and TGF-beta, dependent on Smad-, Ras-, and PI3-kinase-signaling, and likewise modulated by subsequent HGF- and EGF-signaling. Together, our results indicate that MMP-9 levels in tumor fibroblasts are regulated by a complex tumor-stroma cross-talk, involving multiple ligands and cellular signaling pathways. JF - Journal of cell science AU - Stuelten, Christina H AU - DaCosta Byfield, Stacey AU - Arany, Praveen R AU - Karpova, Tatiana S AU - Stetler-Stevenson, William G AU - Roberts, Anita B AD - Laboratory of Cell Regulation and Carcinogenesis, CCR, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 2143 EP - 2153 VL - 118 SN - 0021-9533, 0021-9533 KW - Smad3 Protein KW - 0 KW - Smad3 protein, mouse KW - Transforming Growth Factor beta KW - Tumor Necrosis Factor-alpha KW - Epidermal Growth Factor KW - 62229-50-9 KW - Protein Kinases KW - EC 2.7.- KW - Phosphatidylinositol 3-Kinases KW - EC 2.7.1.- KW - Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Index Medicus KW - Animals KW - Coculture Techniques KW - Humans KW - Phosphatidylinositol 3-Kinases -- physiology KW - Cell Line, Tumor KW - Mice KW - Mitogen-Activated Protein Kinases -- physiology KW - Smad3 Protein -- genetics KW - Mice, Knockout KW - Epithelial Cells -- metabolism KW - Neoplasm Transplantation KW - Signal Transduction -- physiology KW - Protein Kinases -- metabolism KW - Epithelial Cells -- secretion KW - Transplantation, Heterologous KW - Stromal Cells -- metabolism KW - Epidermal Growth Factor -- physiology KW - Matrix Metalloproteinase 9 -- metabolism KW - Breast Neoplasms -- pathology KW - Transforming Growth Factor beta -- secretion KW - Breast Neoplasms -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Transforming Growth Factor beta -- metabolism KW - Fibroblasts -- metabolism KW - Tumor Necrosis Factor-alpha -- secretion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67830058?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cell+science&rft.atitle=Breast+cancer+cells+induce+stromal+fibroblasts+to+express+MMP-9+via+secretion+of+TNF-alpha+and+TGF-beta.&rft.au=Stuelten%2C+Christina+H%3BDaCosta+Byfield%2C+Stacey%3BArany%2C+Praveen+R%3BKarpova%2C+Tatiana+S%3BStetler-Stevenson%2C+William+G%3BRoberts%2C+Anita+B&rft.aulast=Stuelten&rft.aufirst=Christina&rft.date=2005-05-15&rft.volume=118&rft.issue=&rft.spage=2143&rft.isbn=&rft.btitle=&rft.title=Journal+of+cell+science&rft.issn=00219533&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-11 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - SLC18A2 promoter haplotypes and identification of a novel protective factor against alcoholism. AN - 67829328; 15829504 AB - The vesicular monoamine transporter 2 (VMAT2, SLC18A2) takes up cytosolic monoamines into intracellular secretory vesicles, preventing their neurotoxicity in the cytosol and discharging them into extracellular space by exocytosis. It has been shown that one-copy deletion of the VMAT2 gene increases locomotion activity significantly in response to drug treatments and dopamine neuron death rate in response to neurotoxin treatments in knockout mice. Little is known about promoter polymorphisms and their influence on SLC18A2 promoter activity. We have re-sequenced a 17.4 kb DNA in the SLC18A2 promoter region for Caucasians and revealed 47 polymorphisms that confer 13 haplotypes. One of the haplotypes reaches a frequency as high as 65%, likely due to positive selection. In vitro analysis showed a 20% difference in promoter activity between two frequent haplotypes and identified some of the polymorphisms that influence promoter activity. Four haplotype-defining single nucleotide polymorphisms (hdSNPs) can define the frequent haplotypes and by genotyping these hdSNPs, we find that haplotypes with -14234G and -2504C of SLC18A2 promoter region represent a protective factor against alcoholism (P = 0.0038 by Fisher's exact tests). Therefore, SLC18A2 promoter haplotypes defined here create a foundation for transcriptional characterization of individuality and for association study on monoamine-related human diseases. JF - Human molecular genetics AU - Lin, Zhicheng AU - Walther, Donna AU - Yu, Xiao-Ying AU - Li, Suxia AU - Drgon, Tomas AU - Uhl, George R AD - Molecular Neurobiology Branch, Baltimore, MD 21224, USA. zlin@intra.nida.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1393 EP - 1404 VL - 14 IS - 10 SN - 0964-6906, 0964-6906 KW - Membrane Glycoproteins KW - 0 KW - Membrane Transport Proteins KW - SLC18A2 protein, human KW - Slc18a2 protein, mouse KW - Vesicular Biogenic Amine Transport Proteins KW - Vesicular Monoamine Transport Proteins KW - Index Medicus KW - Animals KW - Haplotypes KW - Polymorphism, Genetic KW - Humans KW - Mice KW - Selection, Genetic KW - Promoter Regions, Genetic KW - Alcoholism -- genetics KW - Membrane Transport Proteins -- metabolism KW - Membrane Transport Proteins -- genetics KW - Membrane Glycoproteins -- metabolism KW - Membrane Glycoproteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829328?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+molecular+genetics&rft.atitle=SLC18A2+promoter+haplotypes+and+identification+of+a+novel+protective+factor+against+alcoholism.&rft.au=Lin%2C+Zhicheng%3BWalther%2C+Donna%3BYu%2C+Xiao-Ying%3BLi%2C+Suxia%3BDrgon%2C+Tomas%3BUhl%2C+George+R&rft.aulast=Lin&rft.aufirst=Zhicheng&rft.date=2005-05-15&rft.volume=14&rft.issue=10&rft.spage=1393&rft.isbn=&rft.btitle=&rft.title=Human+molecular+genetics&rft.issn=09646906&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microarray analysis of altered gene expression in murine fibroblasts transformed by nickel(II) to nickel(II)-resistant malignant phenotype. AN - 67817290; 15885260 AB - B200 cells are Ni(II)-transformed mouse BALB/c-3T3 fibroblasts displaying a malignant phenotype and increased resistance to Ni(II) toxicity. In an attempt to find genes whose expression has been altered by the transformation, the Atlas Mouse Stress/Toxicology cDNA Expression Array (Clontech Laboratories, Inc., Palo Alto, CA) was used to analyze the levels of gene expression in both parental and Ni(II)-transformed cells. Comparison of the results revealed a significant up- or downregulation of the expression of 62 of the 588 genes present in the array (approximately 10.5%) in B200 cells. These genes were assigned to different functional groups, including transcription factors and oncogenes (9/14; fractions in parentheses denote the number of up-regulated versus the total number of genes assigned to this group), stress and DNA damage response genes (11/12), growth factors and hormone receptors (6/9), metabolism (7/7), cell adhesion (2/7), cell cycle (3/6), apoptosis (3/4), and cell proliferation (2/3). Among those genes, overexpression of beta-catenin and its downstream targets c-myc and cyclin D1, together with upregulated cyclin G, points at the malignant character of B200 cells. While the increased expression of glutathione (GSH) synthetase, glutathione-S-transferase A4 (GSTA4), and glutathione-S-transferase theta (GSTT), together with high level of several genes responding to oxidative stress, suggests the enforcement of antioxidant defenses in Ni-transformed cells. JF - Toxicology and applied pharmacology AU - Kowara, Renata AU - Karaczyn, Aldona AU - Cheng, Robert Y S AU - Salnikow, Konstantin AU - Kasprzak, Kazimierz S AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, MD 21702, USA. Renata.Kowara@nrc-cnrc.gc.ca Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1 EP - 10 VL - 205 IS - 1 SN - 0041-008X, 0041-008X KW - CTNNB1 protein, mouse KW - 0 KW - Ccng1 protein, mouse KW - Cyclin G KW - Cyclin G1 KW - Cyclins KW - Cytoskeletal Proteins KW - Growth Substances KW - Isoenzymes KW - Membrane Proteins KW - Trans-Activators KW - Transcription Factors, General KW - beta Catenin KW - Nickel KW - 7OV03QG267 KW - Cyclooxygenase 1 KW - EC 1.14.99.1 KW - Cyclooxygenase 2 KW - Prostaglandin-Endoperoxide Synthases KW - Ptgs1 protein, mouse KW - Glutathione Transferase KW - EC 2.5.1.18 KW - Glutathione Synthase KW - EC 6.3.2.3 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Animals KW - Transcription Factors, General -- genetics KW - Cyclins -- drug effects KW - Glutathione Synthase -- genetics KW - Mice, Inbred BALB C KW - Genes, myc -- physiology KW - Apoptosis -- genetics KW - Glutathione -- genetics KW - Oncogenes -- genetics KW - Apoptosis -- drug effects KW - Gene Expression Regulation -- drug effects KW - Genes, myc -- drug effects KW - Cyclins -- genetics KW - Genes, cdc -- drug effects KW - Glutathione -- metabolism KW - Cell Line, Tumor KW - Mice KW - Oxidative Stress -- drug effects KW - Trans-Activators -- metabolism KW - Trans-Activators -- pharmacology KW - DNA Damage -- genetics KW - Growth Substances -- metabolism KW - Genes, bcl-1 -- drug effects KW - Genes, bcl-1 -- physiology KW - Trans-Activators -- genetics KW - Cyclins -- metabolism KW - Prostaglandin-Endoperoxide Synthases -- genetics KW - Cell Line, Transformed KW - Cytoskeletal Proteins -- metabolism KW - Cytoskeletal Proteins -- genetics KW - Cell Proliferation -- drug effects KW - Growth Substances -- genetics KW - Oncogenes -- drug effects KW - Glutathione Transferase -- metabolism KW - Transcription Factors, General -- metabolism KW - Cell Adhesion -- genetics KW - Glutathione Synthase -- metabolism KW - Glutathione Transferase -- genetics KW - Oxidative Stress -- genetics KW - Prostaglandin-Endoperoxide Synthases -- drug effects KW - Gene Expression Regulation -- genetics KW - DNA Damage -- drug effects KW - Transcription Factors, General -- drug effects KW - Glutathione Transferase -- drug effects KW - Cytoskeletal Proteins -- pharmacology KW - Cell Adhesion -- drug effects KW - Glutathione Synthase -- drug effects KW - Phenotype KW - Fibroblasts -- drug effects KW - Fibroblasts -- pathology KW - Microarray Analysis -- trends KW - Nickel -- adverse effects KW - Microarray Analysis -- methods KW - Gene Expression Profiling -- methods KW - Fibroblasts -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67817290?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+applied+pharmacology&rft.atitle=Microarray+analysis+of+altered+gene+expression+in+murine+fibroblasts+transformed+by+nickel%28II%29+to+nickel%28II%29-resistant+malignant+phenotype.&rft.au=Kowara%2C+Renata%3BKaraczyn%2C+Aldona%3BCheng%2C+Robert+Y+S%3BSalnikow%2C+Konstantin%3BKasprzak%2C+Kazimierz+S&rft.aulast=Kowara&rft.aufirst=Renata&rft.date=2005-05-15&rft.volume=205&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+applied+pharmacology&rft.issn=0041008X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-12 N1 - Date created - 2005-05-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Multiple costimulatory modalities enhance CTL avidity. AN - 67806642; 15879092 AB - Recent studies in both animal models and clinical trials have demonstrated that the avidity of T cells is a major determinant of antitumor and antiviral immunity. In this study, we evaluated several different vaccine strategies for their ability to enhance both the quantity and avidity of CTL responses. CD8(+) T cell quantity was measured by tetramer binding precursor frequency, and avidity was measured by both tetramer dissociation and quantitative cytolytic function. We have evaluated a peptide, a viral vector expressing the Ag transgene alone, with one costimulatory molecule (B7-1), and with three costimulatory molecules (B7-1, ICAM-1, and LFA-3), with anti-CTLA-4 mAb, with GM-CSF, and combinations of the above. We have evaluated these strategies in both a foreign Ag model using beta-galactosidase as immunogen, and in a "self" Ag model, using carcinoembryonic Ag as immunogen in carcinoembryonic Ag transgenic mice. The combined use of several of these strategies was shown to enhance not only the quantity, but, to a greater magnitude, the avidity of T cells generated; a combination strategy is also shown to enhance antitumor effects. The results reported in this study thus demonstrate multiple strategies that can be used in both antitumor and antiviral vaccine settings to generate higher avidity host T cell responses. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Hodge, James W AU - Chakraborty, Mala AU - Kudo-Saito, Chie AU - Garnett, Charlie T AU - Schlom, Jeffrey AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 5994 EP - 6004 VL - 174 IS - 10 SN - 0022-1767, 0022-1767 KW - Adjuvants, Immunologic KW - 0 KW - Antibodies, Monoclonal KW - Antigens, CD KW - Antigens, Differentiation KW - CTLA-4 Antigen KW - Cancer Vaccines KW - Carcinoembryonic Antigen KW - Ctla4 protein, mouse KW - Peptide Fragments KW - Granulocyte-Macrophage Colony-Stimulating Factor KW - 83869-56-1 KW - beta-Galactosidase KW - EC 3.2.1.23 KW - Abridged Index Medicus KW - Index Medicus KW - beta-Galactosidase -- administration & dosage KW - Animals KW - Mice, Transgenic KW - Antibodies, Monoclonal -- immunology KW - Granulocyte-Macrophage Colony-Stimulating Factor -- administration & dosage KW - Genetic Vectors KW - Colonic Neoplasms -- pathology KW - Peptide Fragments -- metabolism KW - Carcinoembryonic Antigen -- administration & dosage KW - Carcinoembryonic Antigen -- immunology KW - Combined Modality Therapy KW - Antigens, Differentiation -- immunology KW - Cell Line, Tumor KW - Mice KW - beta-Galactosidase -- immunology KW - Colonic Neoplasms -- immunology KW - Peptide Fragments -- immunology KW - Protein Binding -- immunology KW - Antibodies, Monoclonal -- administration & dosage KW - Mice, Inbred C57BL KW - beta-Galactosidase -- genetics KW - Colonic Neoplasms -- prevention & control KW - Peptide Fragments -- administration & dosage KW - Carcinoembryonic Antigen -- genetics KW - Female KW - Cytotoxicity Tests, Immunologic -- methods KW - Cancer Vaccines -- administration & dosage KW - Lymphocyte Activation -- genetics KW - Cancer Vaccines -- immunology KW - Adjuvants, Immunologic -- administration & dosage KW - Lymphocyte Activation -- immunology KW - Cancer Vaccines -- genetics KW - T-Lymphocytes, Cytotoxic -- immunology KW - Adjuvants, Immunologic -- genetics KW - T-Lymphocytes, Cytotoxic -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67806642?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Multiple+costimulatory+modalities+enhance+CTL+avidity.&rft.au=Hodge%2C+James+W%3BChakraborty%2C+Mala%3BKudo-Saito%2C+Chie%3BGarnett%2C+Charlie+T%3BSchlom%2C+Jeffrey&rft.aulast=Hodge&rft.aufirst=James&rft.date=2005-05-15&rft.volume=174&rft.issue=10&rft.spage=5994&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer Res. 2001 Oct 15;61(20):7568-76 [11606396] J Immunol. 2001 Nov 15;167(10):5824-31 [11698456] J Immunol. 2002 Feb 1;168(3):1167-71 [11801651] Immunity. 2002 Jan;16(1):23-35 [11825563] Nat Rev Immunol. 2001 Dec;1(3):209-19 [11905830] J Immunol. 2002 Jul 1;169(1):531-9 [12077285] Nat Immunol. 2002 Jul;3(7):611-8 [12087419] Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9358-63 [12093915] Cancer Res. 2002 Oct 15;62(20):5770-7 [12384537] J Immunol. 2003 Mar 1;170(5):2523-30 [12594278] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8372-7 [12826605] J Immunol. 2003 Sep 1;171(5):2427-34 [12928390] Nat Med. 2003 Nov;9(11):1377-82 [14528297] Clin Cancer Res. 2004 Feb 1;10(3):1090-9 [14871989] Curr HIV Res. 2003 Jul;1(3):287-94 [15046253] J Virol. 2004 Jul;78(13):7052-60 [15194781] Cancer Res. 1991 Jul 15;51(14):3657-62 [1712245] J Natl Cancer Inst. 1992 Jul 15;84(14):1084-91 [1619682] Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3539-43 [8097319] Cancer Res. 1994 Aug 1;54(15):4169-76 [8033149] J Exp Med. 1995 Aug 1;182(2):459-65 [7543139] Nature. 1995 Sep 28;377(6547):348-51 [7566090] Science. 1996 Mar 22;271(5256):1734-6 [8596936] Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4102-7 [8633023] Blood. 1996 Jul 1;88(1):202-10 [8704175] Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10972-7 [8855293] Methods. 1997 Jun;12(2):117-23 [9184376] Cancer Res. 1997 Sep 15;57(18):4036-41 [9307290] Ann Surg Oncol. 1997 Oct-Nov;4(7):579-85 [9367025] Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10067-71 [9707601] J Clin Invest. 1998 Sep 15;102(6):1239-48 [9739058] Novartis Found Symp. 1998;215:92-8; discussion 98-102, 186-90 [9760573] J Immunol. 1999 Jan 15;162(2):989-94 [9916724] Cancer Res. 1999 Feb 1;59(3):676-83 [9973217] J Immunol. 1999 Feb 15;162(4):2227-34 [9973498] Vaccine. 1999 Feb 26;17(7-8):893-903 [10067696] Cancer Gene Ther. 1999 Jan-Feb;6(1):89-95 [10078968] Cancer Immunol Immunother. 1999 May-Jun;48(2-3):123-31 [10414466] J Exp Med. 1999 Aug 2;190(3):355-66 [10430624] J Clin Oncol. 2005 Feb 1;23(4):720-31 [15613691] Immunol Rev. 1999 Aug;170:151-72 [10566149] Cancer Res. 1999 Nov 15;59(22):5800-7 [10582702] J Immunol. 2000 Jan 1;164(1):191-200 [10605011] Cancer Res. 2000 May 1;60(9):2444-8 [10811122] Cytokine. 2000 Jul;12(7):960-71 [10880241] J Exp Med. 2000 Jul 17;192(2):295-302 [10899916] J Natl Cancer Inst. 2000 Aug 2;92(15):1228-39 [10922408] Cancer Res. 2001 Jan 1;61(1):206-14 [11196163] Eur J Immunol. 2001 Feb;31(2):412-20 [11180105] J Immunol. 2001 Feb 1;166(3):1690-7 [11160212] J Immunol. 2001 Mar 15;166(6):3908-14 [11238635] Int Immunol. 2001 Jun;13(6):817-24 [11369710] Int Immunol. 2001 Jul;13(7):897-908 [11431420] Cancer Res. 2001 Aug 1;61(15):5850-6 [11479225] J Exp Med. 2001 Aug 20;194(4):481-9 [11514604] Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10302-7 [11517329] J Exp Med. 2001 Sep 17;194(6):823-32 [11560997] J Exp Med. 2001 Sep 17;194(6):833-46 [11560998] J Virol. 2001 Nov;75(21):10065-72 [11581375] Erratum In: J Immunol. 2005 Jun 15;174(12):8220 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling. AN - 67806210; 15879121 AB - Phospholipase Cgamma (PLCgamma) is a ubiquitous gatekeeper of calcium mobilization and diacylglycerol-mediated events induced by the activation of Ag and growth factor receptors. The activity of PLCgamma is regulated through its controlled membrane translocation and tyrosine (Y) phosphorylation. Four activation-induced tyrosine phosphorylation sites have been previously described (Y472, Y771, Y783, and Y1254), but their specific roles in Ag receptor-induced PLCgamma1 activation are not fully elucidated. Unexpectedly, we found that the phosphorylation of a PLCgamma1 construct with all four sites mutated to phenylalanine was comparable with that observed with wild-type PLCgamma1, suggesting the existence of an unidentified site(s). Sequence alignment with known phosphorylation sites in PLCgamma2 indicated homology of PLCgamma1 tyrosine residue 775 (Y775) with PLCgamma2 Y753, a characterized phosphorylation site. Tyrosine 775 was characterized as a phosphorylation site using phospho-specific anti-Y775 antiserum, and by mutational analysis. Phosphorylation of Y775 did not depend on the other tyrosines, and point mutation of PLCgamma1 Y775, or the previously described Y783, substantially reduced AgR-induced calcium, NF-AT, and AP-1 activation. Mutation of Y472, Y771, and Y1254 had no effect on overall PLCgamma1 phosphorylation or activation. Although the concomitant mutation of Y775 and Y783 abolished downstream PLCgamma1 signaling, these two tyrosines were sufficient to reconstitute the wild-type response in the absence of functional Y472, Y771, and Y1254. These data establish Y775 as a critical phosphorylation site for PLCgamma1 activation and confirm the functional importance of Y783. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Serrano, Carmen J AU - Graham, Laurie AU - DeBell, Karen AU - Rawat, Rashmi AU - Veri, Maria Concetta AU - Bonvini, Ezio AU - Rellahan, Barbara L AU - Reischl, Ilona G AD - Division of Monoclonal Antibodies, Office of Biotechnology Products, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, National Institutes of Health Campus, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 6233 EP - 6237 VL - 174 IS - 10 SN - 0022-1767, 0022-1767 KW - DNA-Binding Proteins KW - 0 KW - Diglycerides KW - Isoenzymes KW - NFATC Transcription Factors KW - Nuclear Proteins KW - Receptors, Antigen, B-Cell KW - Receptors, Antigen, T-Cell KW - Transcription Factor AP-1 KW - Transcription Factors KW - Tyrosine KW - 42HK56048U KW - Type C Phospholipases KW - EC 3.1.4.- KW - Phospholipase C gamma KW - EC 3.1.4.3 KW - Calcium KW - SY7Q814VUP KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Transcription Factors -- metabolism KW - Transcription Factor AP-1 -- metabolism KW - Humans KW - Jurkat Cells KW - Amino Acid Sequence KW - Isoenzymes -- genetics KW - Isoenzymes -- metabolism KW - Mutagenesis, Site-Directed KW - Calcium -- metabolism KW - Cattle KW - Isoenzymes -- deficiency KW - Phosphorylation KW - Transfection KW - Molecular Sequence Data KW - Diglycerides -- physiology KW - Nuclear Proteins -- metabolism KW - Cell Line KW - DNA-Binding Proteins -- metabolism KW - Receptors, Antigen, T-Cell -- metabolism KW - Signal Transduction -- genetics KW - Signal Transduction -- immunology KW - Tyrosine -- genetics KW - Tyrosine -- metabolism KW - Type C Phospholipases -- deficiency KW - Receptors, Antigen, B-Cell -- physiology KW - Type C Phospholipases -- genetics KW - Receptors, Antigen, T-Cell -- physiology KW - Type C Phospholipases -- metabolism KW - Receptors, Antigen, B-Cell -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67806210?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=A+new+tyrosine+phosphorylation+site+in+PLC+gamma+1%3A+the+role+of+tyrosine+775+in+immune+receptor+signaling.&rft.au=Serrano%2C+Carmen+J%3BGraham%2C+Laurie%3BDeBell%2C+Karen%3BRawat%2C+Rashmi%3BVeri%2C+Maria+Concetta%3BBonvini%2C+Ezio%3BRellahan%2C+Barbara+L%3BReischl%2C+Ilona+G&rft.aulast=Serrano&rft.aufirst=Carmen&rft.date=2005-05-15&rft.volume=174&rft.issue=10&rft.spage=6233&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Impact of chronic GVHD therapy on the development of squamous-cell cancers after hematopoietic stem-cell transplantation: an international case-control study. AN - 67790044; 15687239 AB - Previous studies of recipients of hematopoietic stem-cell transplants suggest that graft-versus-host disease (GVHD) and its therapy may increase the risk for solid cancers, particularly squamous-cell carcinomas (SCCs) of the buccal cavity and skin. However, the importance and magnitude of these associations are not well characterized. We conducted a case-control study of 183 patients with posttransplantation solid cancers (58 SCCs, 125 non-SCCs) and 501 matched control patients within a cohort of 24,011 patients who underwent hematopoietic stem-cell transplantation (HSCT) at 215 centers worldwide. Our results showed that chronic GVHD and its therapy were strongly related to the risk for SCC, whereas no increase in risk was found for non-SCCs. Major risk factors for the development of SCC were long duration of chronic GVHD therapy (P < .001); use of azathioprine, particularly when combined with cyclosporine and steroids (P < .001); and severe chronic GVHD (P = .004). Given that most patients who received prolonged immunosuppressive therapy and those with severe chronic GVHD were also treated with azathioprine, the independent effects of these factors could not be evaluated. Additional analyses determined that prolonged immunosuppressive therapy and azathioprine use were also significant risk factors for SCC of the skin and of the oral mucosa. These data provide further encouragement for strategies to prevent chronic GVHD and for the development of more effective and less carcinogenic treatment regimens for patients with moderate or severe chronic GVHD. Our results also suggest that clinical screening for SCC is appropriate among patients exposed to persistent chronic GVHD, prolonged immunosuppressive therapy, or both. JF - Blood AU - Curtis, Rochelle E AU - Metayer, Catherine AU - Rizzo, J Douglas AU - Socié, Gérard AU - Sobocinski, Kathleen A AU - Flowers, Mary E D AU - Travis, William D AU - Travis, Lois B AU - Horowitz, Mary M AU - Deeg, H Joachim AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Executive Plaza South, Suite 7042, 6120 Executive Blvd, Bethesda, MD 20892, USA. rcurtis@mail.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 3802 EP - 3811 VL - 105 IS - 10 SN - 0006-4971, 0006-4971 KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Child KW - Internationality KW - Child, Preschool KW - Infant KW - Immunosuppression -- adverse effects KW - Risk Factors KW - Adult KW - Case-Control Studies KW - Middle Aged KW - Follow-Up Studies KW - Adolescent KW - Time Factors KW - Female KW - Male KW - Neoplasms, Squamous Cell -- pathology KW - Neoplasms, Squamous Cell -- therapy KW - Neoplasms, Squamous Cell -- immunology KW - Graft vs Host Disease -- immunology KW - Graft vs Host Disease -- therapy KW - Hematopoietic Stem Cell Transplantation KW - Neoplasms, Squamous Cell -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67790044?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Effect+of+topical+iodine+treatment+on+early+sulfur-mustard-induced+cutaneous+changes+and+epidermal-cell+proliferation&rft.au=Trivedi%2C+S%3BWormser%2C+U%3BFlagler%2C+N%3BBrodsky%2C+B%3BPeddada%2C+S%3BNyska%2C+A&rft.aulast=Trivedi&rft.aufirst=S&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Blood. 1999 Oct 1;94(7):2208-16 [10498590] Stat Med. 1999 Mar 30;18(6):695-706 [10204198] Ann Intern Med. 1999 Nov 16;131(10):738-44 [10577296] J Natl Cancer Inst. 1999 Dec 1;91(23):1992-3 [10580016] J Clin Oncol. 2000 Jan;18(2):348-57 [10637249] Blood. 2000 Jun 15;95(12):3702-9 [10845900] Lancet. 2000 May 27;355(9218):1886-7 [10866449] J Clin Oncol. 2001 Jan 15;19(2):464-71 [11208840] Blood. 2002 Jul 15;100(2):406-14 [12091329] Blood. 2002 Aug 1;100(3):761-7 [12130483] Blood Rev. 2002 Jun;16(2):135-46 [12127957] Nature. 2002 Dec 19-26;420(6917):860-7 [12490959] Blood. 2003 Feb 1;101(3):822-6 [12393424] J Clin Oncol. 2003 Apr 1;21(7):1352-8 [12663726] Transpl Int. 2003 Mar;16(3):146-53 [12664208] N Engl J Med. 2003 Apr 24;348(17):1681-91 [12711744] Cancer Metastasis Rev. 2003 Mar;22(1):95-102 [12716041] Br J Cancer. 2003 Oct 6;89(7):1221-7 [14520450] J Natl Cancer Inst. 2003 Dec 3;95(23):1772-83 [14652239] Lancet. 1984 Mar 17;1(8377):583-7 [6142304] Blood. 1988 Aug;72(2):546-54 [3042041] Cancer. 1990 Feb 1;65(3):473-6 [2297638] Blood. 1991 Jul 15;78(2):277-9 [2070065] Leuk Lymphoma. 1992 Aug;7(5-6):419-23 [1493443] N Engl J Med. 1993 Oct 14;329(16):1152-7 [8377778] J Clin Oncol. 1994 Oct;12(10):2187-92 [7931488] Transplantation. 1996 Mar 15;61(5):715-21 [8607173] Blood. 1996 Jan 1;87(1):386-92 [8547667] Lancet. 1995 Aug 12;346(8972):403-6 [7623570] N Engl J Med. 1997 Mar 27;336(13):897-904 [9070469] Clin Dermatol. 1997 May-Jun;15(3):427-37 [9255448] Blood. 1998 Mar 15;91(6):1833-44 [9490664] Lancet. 1998 Feb 28;351(9103):623-8 [9500317] J Am Acad Dermatol. 1999 Feb;40(2 Pt 1):177-86 [10025742] Nature. 1999 Feb 11;397(6719):530-4 [10028970] J Clin Oncol. 1999 Oct;17(10):3122-7 [10506608] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitric oxide-induced resistance to hydrogen peroxide stress is a glutamate cysteine ligase activity-dependent process. AN - 67788397; 15855054 AB - Nitric oxide (*NO) is a reactive nitrogen species known to be involved in cytotoxic processes. Cells respond to cytotoxic injury by stress response induction leading to the development of cellular resistance. This report describes an *NO-induced stress response in Chinese hamster fibroblasts (HA1), which leads to glutathione synthesis-dependent resistance to H2O2-mediated oxidative stress. The development of resistance to H2O2 was completely abolished by the inhibition of glutamate cysteine ligase (GCL) during the first 8 h of recovery after *NO exposure. Altered thiol metabolism was observed immediately after *NO exposure as demonstrated by up to 75% decrease in intracellular thiol pools (glutathione, gamma-glutamylcysteine, and cysteine), which then reaccumulated during the *NO-mediated development of resistance. Immunoreactive protein and activity associated with GCL decreased immediately after exposure to *NO and then reaccumulated during the development of resistance to H2O2 challenge. Moreover, compared to N2 controls the activity levels of GCL in *NO-exposed cells increased approximately twofold 24 h after H2O2 challenge. These results demonstrate that *NO exposure is capable of inducing an adaptive response to H2O2-mediated oxidative stress in mammalian cells, which involves alterations in thiol metabolism and is dependent upon glutathione synthesis and increased GCL activity. JF - Free radical biology & medicine AU - Ridnour, Lisa A AU - Sim, Julia E AU - Choi, Jinah AU - Dickinson, Dale A AU - Forman, Henry J AU - Ahmad, Iman M AU - Coleman, Mitchell C AU - Hunt, Clayton R AU - Goswami, Prahbat C AU - Spitz, Douglas R AD - Division of Radiation and Cancer Biology, Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park Boulevard, Room 411, St. Louis, MO 63108, USA. ridnourl@mail.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1361 EP - 1371 VL - 38 IS - 10 SN - 0891-5849, 0891-5849 KW - Free Radical Scavengers KW - 0 KW - Oxidants KW - Sulfhydryl Compounds KW - Nitric Oxide KW - 31C4KY9ESH KW - Hydrogen Peroxide KW - BBX060AN9V KW - Glutamate-Cysteine Ligase KW - EC 6.3.2.2 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Oxidation-Reduction KW - Animals KW - Sulfhydryl Compounds -- metabolism KW - Cricetulus KW - Cells, Cultured KW - Glutathione -- metabolism KW - Cricetinae KW - Fibroblasts -- drug effects KW - Fibroblasts -- enzymology KW - Glutamate-Cysteine Ligase -- antagonists & inhibitors KW - Oxidants -- pharmacology KW - Oxidative Stress KW - Hydrogen Peroxide -- pharmacology KW - Nitric Oxide -- pharmacology KW - Fibroblasts -- cytology KW - Glutamate-Cysteine Ligase -- metabolism KW - Free Radical Scavengers -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67788397?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Nitric+oxide-induced+resistance+to+hydrogen+peroxide+stress+is+a+glutamate+cysteine+ligase+activity-dependent+process.&rft.au=Ridnour%2C+Lisa+A%3BSim%2C+Julia+E%3BChoi%2C+Jinah%3BDickinson%2C+Dale+A%3BForman%2C+Henry+J%3BAhmad%2C+Iman+M%3BColeman%2C+Mitchell+C%3BHunt%2C+Clayton+R%3BGoswami%2C+Prahbat+C%3BSpitz%2C+Douglas+R&rft.aulast=Ridnour&rft.aufirst=Lisa&rft.date=2005-05-15&rft.volume=38&rft.issue=10&rft.spage=1361&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Invasion of Human Tissue Ex Vivo by Borrelia burgdorferi AN - 17505003; 6403552 AB - Borrelia burgdorferi sensu stricto is an etiological agent of Lyme disease. The lack of an adequate ex vivo system for human tissue infection is an obstacle to fully understanding the molecular mechanisms of invasion of tissue by B. burgdorferi and its adaptation within the human host. Here, we report on the development of such a system. We inoculated blocks of human tonsillar tissue with B. burgdorferi spirochetes, cultured them in a low-shear rotating wall vessel (RWV) bioreactor, and analyzed them using light and electron microscopy, nested polymerase chain reaction (PCR), and quantitative real-time PCR. Also, we evaluated the expression of the outer surface proteins (Osps) OspA and OspC by use of quantitative Western blotting. Light and electron microscopic analysis revealed multiple spirochetes localized extracellularly within the tissue, and their identity was confirmed by PCR. Quantification of spirochetes inside the RWV-cultured tonsillar tissue demonstrated that the number of B. burgdorferi exceeded the initial inoculum by an order of magnitude, indicating that spirochetes replicated in the tissue. Electron microscopic analysis showed that some spirochetes were arranged in cystic structures and that invading spirochetes differentially expressed surface proteins; both of these features have been described for infected tissues in vivo. The system we have developed can be used to study B. burgdorferi pathogenesis under controlled conditions ex vivo, in particular to explore the gene activation responsible for the adaptation of B. burgdorferi to human tissue that leads to Lyme disease. JF - Journal of Infectious Diseases AU - Duray, PH AU - Yin, S-R AU - Ito, Y AU - Bezrukov, L AU - Cox, C AU - Cho, M-S AU - Fitzgerald, W AU - Dorward, D AU - Zimmerberg, J AU - Margolis, L AD - Department of Pathology, National Cancer Institute, National Institutes of Health, and National Aeronautics and Space Administration/National Institutes of Health Center for Three-Dimensional Tissue Culture, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1747 EP - 1754 VL - 191 IS - 10 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology KW - Molecular modelling KW - Adaptations KW - outer surface proteins KW - Borrelia burgdorferi KW - Spirochetes KW - Tonsil KW - Bioreactors KW - Controlled conditions KW - Polymerase chain reaction KW - Electron microscopy KW - Transcription activation KW - Lyme disease KW - J 02855:Human Bacteriology: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17505003?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Invasion+of+Human+Tissue+Ex+Vivo+by+Borrelia+burgdorferi&rft.au=Duray%2C+PH%3BYin%2C+S-R%3BIto%2C+Y%3BBezrukov%2C+L%3BCox%2C+C%3BCho%2C+M-S%3BFitzgerald%2C+W%3BDorward%2C+D%3BZimmerberg%2C+J%3BMargolis%2C+L&rft.aulast=Duray&rft.aufirst=PH&rft.date=2005-05-15&rft.volume=191&rft.issue=10&rft.spage=1747&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Borrelia burgdorferi; Spirochetes; Polymerase chain reaction; Lyme disease; Tonsil; Adaptations; Transcription activation; Molecular modelling; outer surface proteins; Bioreactors; Controlled conditions; Electron microscopy ER - TY - JOUR T1 - Protein kinase C-dependent regulation of NAG-1/placental bone morphogenic protein/MIC-1 expression in LNCaP prostate carcinoma cells. AN - 67804196; 15757899 AB - NAG-1 (nonsteroidal anti-inflammatory drug-activated gene), a member of the transforming growth factor beta superfamily, is involved in cellular processes such as inflammation, apoptosis/survival, and tumorigenesis and is regulated by p53, Sp1, and Egr-1. In the current study, the regulation of NAG-1 expression in LNCaP human prostate carcinoma cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. TPA treatment increased NAG-1 protein and mRNA levels in a time- and concentration-dependent manner as well as NF-kappa B binding/transcriptional activity in LNCaP cells. Pretreatment with protein kinase C (PKC) inhibitor blocked the TPA-induced increase in NAG-1 protein levels and NF-kappa B binding/transcriptional activity, whereas an inhibition of p38, JNK, MEK activity had no effect on TPA-induced NAG-1 levels and NF-kappa B transcriptional activity. Expression of constitutively active PKCs induced an increase in NF-kappa B transcriptional activity and NAG-1 protein levels in LNCaP cells. The expression of NF-kappa B p65 induced NAG-1 promoter activity, and chromatin immunoprecipitation assay for p65 showed that NF-kappa B binds the NAG-1 promoter in LNCaP cells. Inhibition of TPA-induced NAG-1 expression by NAG-1 short interfering RNA blocked TPA-induced apoptosis in LNCaP cells, suggesting induction of NAG-1 negatively affects LNCaP cell survival. These results demonstrate that NAG-1 expression is up-regulated by TPA in LNCaP cells through a PKC-dependent pathway involving the activation of NF-kappa B. JF - The Journal of biological chemistry AU - Shim, Minsub AU - Eling, Thomas E AD - Eicosanoids Biochemistry Section, Laboratory of Molecular Carcinogenesis, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/05/13/ PY - 2005 DA - 2005 May 13 SP - 18636 EP - 18642 VL - 280 IS - 19 SN - 0021-9258, 0021-9258 KW - Carcinogens KW - 0 KW - Cytokines KW - GDF15 protein, human KW - Growth Differentiation Factor 15 KW - NF-kappa B KW - Protein Isoforms KW - RNA, Messenger KW - RNA, Small Interfering KW - Luciferases KW - EC 1.13.12.- KW - Protein Kinase C KW - EC 2.7.11.13 KW - JNK Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - p38 Mitogen-Activated Protein Kinases KW - MAP Kinase Kinase Kinase 1 KW - EC 2.7.11.25 KW - MAP Kinase Kinase 4 KW - EC 2.7.12.2 KW - Mitogen-Activated Protein Kinase Kinases KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Blotting, Northern KW - Apoptosis KW - Cell Nucleus -- metabolism KW - Humans KW - MAP Kinase Kinase Kinase 1 -- metabolism KW - Transcription, Genetic KW - RNA, Small Interfering -- metabolism KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Mitogen-Activated Protein Kinase Kinases -- metabolism KW - Cell Survival KW - Promoter Regions, Genetic KW - Chromatin Immunoprecipitation KW - Time Factors KW - Male KW - Dose-Response Relationship, Drug KW - Luciferases -- metabolism KW - Cell Line, Tumor KW - Inflammation KW - Blotting, Western KW - RNA, Messenger -- metabolism KW - Transfection KW - JNK Mitogen-Activated Protein Kinases -- metabolism KW - NF-kappa B -- metabolism KW - Protein Kinase C -- metabolism KW - Prostatic Neoplasms -- metabolism KW - Cytokines -- genetics KW - Cytokines -- biosynthesis KW - Protein Kinase C -- chemistry KW - Gene Expression Regulation KW - Protein Kinase C -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67804196?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C-dependent+regulation+of+NAG-1%2Fplacental+bone+morphogenic+protein%2FMIC-1+expression+in+LNCaP+prostate+carcinoma+cells.&rft.au=Shim%2C+Minsub%3BEling%2C+Thomas+E&rft.aulast=Shim&rft.aufirst=Minsub&rft.date=2005-05-13&rft.volume=280&rft.issue=19&rft.spage=18636&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-12 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene expression profiling identifies IL-13 receptor alpha 2 chain as a therapeutic target in prostate tumor cells overexpressing adrenomedullin. AN - 67530898; 15609296 AB - Human adrenomedullin (AM) is a 52 amino acid peptide, which shares homology with the calcitonin gene-related peptide. Overexpression of AM in the prostate carcinoma cell line PC-3 results in growth inhibition with a 20% (for human AM) and 35% (for rat AM) increase in doubling time compared to parental or mock-transfected cells. We demonstrate by gene expression profiling that AM overexpression results in the dysregulation of approximately 100 genes. Examples of such genes include many involved in the formation of the cytoskeleton, cell adhesion and the extracellular matrix, as well as regulators of the cell cycle and apoptosis, cytokines and transcription factors. Several genes related to cell growth arrest, such as GADD45, IGF-BP6 and RUNX-3, are upregulated by AM. Interestingly, interleukin-13 receptor alpha 2 (IL-13R alpha 2) transcripts were significantly increased in clones overexpressing AM, which was confirmed by semiquantitative RT-PCR analysis. In addition, PC-3 cells treated with AM showed an overexpression of IL-13R alpha 2, which was abolished when cells were preincubated with an anti-AM blocking antibody. When PC-3 cells overexpressing AM and the IL-13R alpha 2 were treated with the highly specific IL13-PE38 cytotoxin, which binds to this receptor, a concentration-dependent inhibition of protein synthesis was observed. The IC(50) (concentration of cytotoxin inhibiting protein synthesis by 50%) ranged from 1 to 4 ng/ml. This cytotoxicity was specific as it was neutralized by the excess of IL-13 and confirmed by clonogenic assays. This study describes a novel AM-induced mechanism of tumor sensitization through the upregulation of functional IL-13R alpha 2 chain, an ideal target for the highly specific recombinant chimeric cytotoxin IL13-PE38. JF - International journal of cancer AU - Gonzalez-Moreno, Oscar AU - Calvo, Alfonso AU - Joshi, Bharat H AU - Abasolo, Ibane AU - Leland, Pamela AU - Wang, Zhou AU - Montuenga, Luis AU - Puri, Raj K AU - Green, Jeffrey E AD - Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/10/ PY - 2005 DA - 2005 May 10 SP - 870 EP - 878 VL - 114 IS - 6 SN - 0020-7136, 0020-7136 KW - IL13RA1 protein, human KW - 0 KW - Il13ra1 protein, rat KW - Interleukin-13 Receptor alpha1 Subunit KW - Peptides KW - Receptors, Interleukin KW - Receptors, Interleukin-13 KW - Adrenomedullin KW - 148498-78-6 KW - Index Medicus KW - Apoptosis KW - Tumor Cells, Cultured KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Up-Regulation KW - Reverse Transcriptase Polymerase Chain Reaction KW - Cell Cycle KW - Male KW - Prostatic Neoplasms -- pathology KW - Gene Expression Profiling KW - Receptors, Interleukin -- genetics KW - Peptides -- analysis KW - Prostatic Neoplasms -- genetics KW - Receptors, Interleukin -- physiology KW - Receptors, Interleukin -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67530898?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Relationship+of+treatment-related+decreases+in+serum+alkaline+phosphatase+activity+with+alterations+in+body+weight+for+rats+in+13-week+toxicity+studies&rft.au=Betz%2C+L%3BHarvey%2C+E%3BTravlos%2C+G&rft.aulast=Betz&rft.aufirst=L&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-16 N1 - Date created - 2005-03-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long CGG-repeat tracts are toxic to human cells: implications for carriers of Fragile X premutation alleles. AN - 67803460; 15862312 AB - People with 59-200 CGG.CCG-repeats in the 5' UTR of one of their FMR1 genes are at risk for Fragile X tremor and ataxia syndrome. Females are also at risk for premature ovarian failure. These symptoms are thought to be due to the presence of the repeats at the DNA and/or RNA level. We show here that long transcribed but untranslated CGG-repeat tracts are toxic to human cells and alter the expression of a wide variety of different genes including caspase-8, CYFIP, Neurotensin and UBE3A. JF - FEBS letters AU - Handa, Vaishali AU - Goldwater, Deena AU - Stiles, David AU - Cam, Margaret AU - Poy, George AU - Kumari, Daman AU - Usdin, Karen AD - Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA. Y1 - 2005/05/09/ PY - 2005 DA - 2005 May 09 SP - 2702 EP - 2708 VL - 579 IS - 12 SN - 0014-5793, 0014-5793 KW - 5' Untranslated Regions KW - 0 KW - Adaptor Proteins, Signal Transducing KW - Annexin A5 KW - CYFIP1 protein, human KW - Culture Media, Serum-Free KW - Herbicides KW - Neurotensin KW - 39379-15-2 KW - UBE3A protein, human KW - EC 2.3.2.26 KW - Ubiquitin-Protein Ligases KW - EC 2.3.2.27 KW - CASP8 protein, human KW - EC 3.4.22.- KW - Caspase 8 KW - Caspases KW - Staurosporine KW - H88EPA0A3N KW - Doxycycline KW - N12000U13O KW - Paraquat KW - PLG39H7695 KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Doxycycline -- pharmacology KW - Caspases -- genetics KW - Annexin A5 -- metabolism KW - Annexin A5 -- drug effects KW - Caspases -- metabolism KW - Cell Survival KW - Adaptor Proteins, Signal Transducing -- metabolism KW - Ataxia -- genetics KW - Ubiquitin-Protein Ligases -- genetics KW - Gene Expression Regulation KW - Paraquat -- pharmacology KW - Dose-Response Relationship, Drug KW - Caspases -- analysis KW - Neurotensin -- metabolism KW - Neurotensin -- genetics KW - Gene Expression Profiling KW - Staurosporine -- pharmacology KW - Blotting, Western KW - Herbicides -- pharmacology KW - Adaptor Proteins, Signal Transducing -- genetics KW - Ubiquitin-Protein Ligases -- metabolism KW - Mutation KW - Cell Line KW - Female KW - Trinucleotide Repeat Expansion -- genetics KW - Alleles KW - Fragile X Syndrome -- genetics KW - Heterozygote KW - Trinucleotide Repeat Expansion -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67803460?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+letters&rft.atitle=Long+CGG-repeat+tracts+are+toxic+to+human+cells%3A+implications+for+carriers+of+Fragile+X+premutation+alleles.&rft.au=Handa%2C+Vaishali%3BGoldwater%2C+Deena%3BStiles%2C+David%3BCam%2C+Margaret%3BPoy%2C+George%3BKumari%2C+Daman%3BUsdin%2C+Karen&rft.aulast=Handa&rft.aufirst=Vaishali&rft.date=2005-05-09&rft.volume=579&rft.issue=12&rft.spage=2702&rft.isbn=&rft.btitle=&rft.title=FEBS+letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-09 N1 - Date created - 2005-05-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Different vanilloid agonists cause different patterns of calcium response in CHO cells heterologously expressing rat TRPV1. AN - 67724522; 15820503 AB - The vanilloid receptor subtype 1 (TRPV1 or VR1) is expressed in nociceptive primary afferents of the C-fiber 'pain' pathway and has attracted considerable attention as a therapeutic target. Here, using rat TRPV1 heterologously expressed in Chinese hamster ovary cells, we show that different agonists show different patterns of modulation of the intracellular Ca2+ concentration, monitored in individual cells by fura-2 Ca2+ imaging. We identified 5 parameters (potency, maximal response, latency of response, variability of latency of response among individual cells, and desensitization) which behaved differently for different compounds. The potencies of the compounds examined ranged from EC50 values of 80 pM to 9 microM. Peak levels of induced [Ca2+]i were observed either higher (RTX) or lower (anandamide) than for capsaicin. Significant latencies of response were observed for some (e.g. RTX) but not other derivatives, with great variation among individual cells in this latency. Marked desensitization after stimulation was detected in some cases (e.g. anandamide, capsaicin); for others, no desensitization was observed. We conclude that structurally diverse vanilloid agonists induce marked diversity in the patterns of Ca2+ response. This diversity of response may provide opportunities for pharmacological exploitation. JF - Life sciences AU - Tóth, Attila AU - Wang, Yun AU - Kedei, Noémi AU - Tran, Richard AU - Pearce, Larry V AU - Kang, Sang-Uk AU - Jin, Mi-Kyung AU - Choi, Hyun-Kyung AU - Lee, Jeewoo AU - Blumberg, Peter M AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bldg. 37, Room 4048, 37 Convent Dr., MSC 4255, Bethesda, MD 20892-4255, United States. Y1 - 2005/05/06/ PY - 2005 DA - 2005 May 06 SP - 2921 EP - 2932 VL - 76 IS - 25 SN - 0024-3205, 0024-3205 KW - Arachidonic Acids KW - 0 KW - Calcium Channel Blockers KW - Diterpenes KW - Endocannabinoids KW - Fluorescent Dyes KW - Ion Channels KW - Polyunsaturated Alkamides KW - TRPV Cation Channels KW - Trpv1 protein, rat KW - resiniferatoxin KW - A5O6P1UL4I KW - Capsaicin KW - S07O44R1ZM KW - Calcium KW - SY7Q814VUP KW - Fura-2 KW - TSN3DL106G KW - anandamide KW - UR5G69TJKH KW - Index Medicus KW - Rats KW - Animals KW - Cricetulus KW - Kinetics KW - CHO Cells KW - Cricetinae KW - Calcium -- metabolism KW - Calcium Channel Blockers -- metabolism KW - Capsaicin -- metabolism KW - Arachidonic Acids -- metabolism KW - Ion Channels -- agonists KW - Diterpenes -- metabolism KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67724522?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Different+vanilloid+agonists+cause+different+patterns+of+calcium+response+in+CHO+cells+heterologously+expressing+rat+TRPV1.&rft.au=T%C3%B3th%2C+Attila%3BWang%2C+Yun%3BKedei%2C+No%C3%A9mi%3BTran%2C+Richard%3BPearce%2C+Larry+V%3BKang%2C+Sang-Uk%3BJin%2C+Mi-Kyung%3BChoi%2C+Hyun-Kyung%3BLee%2C+Jeewoo%3BBlumberg%2C+Peter+M&rft.aulast=T%C3%B3th&rft.aufirst=Attila&rft.date=2005-05-06&rft.volume=76&rft.issue=25&rft.spage=2921&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The GB1 Amyloid Fibril: Recruitment of the Peripheral beta -Strands of the Domain Swapped Dimer into the Polymeric Interface AN - 19809314; 6391087 AB - Three-dimensional domain swapping has been evoked as a mechanism for oligomerization of proteins. Here, we show for the immunoglobulin-binding domain B1 of streptococcal protein G (GB1) that fibril formation is observed readily for variants that exist as domain-swapped dimers. No fibril was formed by a revertant that exhibits the stable wild-type GB1 fold or a mutant comprising a highly destabilized, fluctuating ensemble of conformers. Structural features of the GB1 amyloid fibril were characterized by cysteine disulfide cross-linking. Residues in the outer edge beta -strands of the domain-swapped dimer readily form intermolecular disulfide bonds prior to and during fibril formation. On the basis of these data, a structural model for the assembly of domain-swapped dimers into a polymeric structure of the GB1 fibril is proposed. JF - Journal of Molecular Biology AU - Louis, J M AU - Byeon, IJL AU - Baxa, U AU - Gronenborn, A M AD - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA, gronenborn@nih.gov Y1 - 2005/05/06/ PY - 2005 DA - 2005 May 06 SP - 687 EP - 698 VL - 348 IS - 3 SN - 0022-2836, 0022-2836 KW - Microbiology Abstracts B: Bacteriology KW - Streptococcus KW - Data processing KW - Recruitment KW - Oligomerization KW - Disulfide bonds KW - Models KW - Cysteine KW - Fibrillogenesis KW - streptococcal protein G KW - Revertants KW - Fibrils KW - Amyloid KW - J 02310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19809314?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Molecular+Biology&rft.atitle=The+GB1+Amyloid+Fibril%3A+Recruitment+of+the+Peripheral+beta+-Strands+of+the+Domain+Swapped+Dimer+into+the+Polymeric+Interface&rft.au=Louis%2C+J+M%3BByeon%2C+IJL%3BBaxa%2C+U%3BGronenborn%2C+A+M&rft.aulast=Louis&rft.aufirst=J&rft.date=2005-05-06&rft.volume=348&rft.issue=3&rft.spage=687&rft.isbn=&rft.btitle=&rft.title=Journal+of+Molecular+Biology&rft.issn=00222836&rft_id=info:doi/10.1016%2Fj.jmb.2005.02.071 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Data processing; Cysteine; streptococcal protein G; Fibrillogenesis; Oligomerization; Recruitment; Disulfide bonds; Revertants; Fibrils; Models; Amyloid; Streptococcus DO - http://dx.doi.org/10.1016/j.jmb.2005.02.071 ER - TY - JOUR T1 - Qualitative assessment of IC50 values of inhibitors of the neuronal nicotinic acetylcholine receptor using a single chromatographic experiment and multivariate cluster analysis. AN - 67564153; 15797535 AB - It has been widely demonstrated that affinity chromatography can be used to derive binding affinities, and that these affinities can be correlated to data obtained using standard techniques such as membrane binding, ultrafiltration and equilibrium dialysis. The purpose of this study is to evaluate the use of immobilized nicotinic acetylcholine receptor stationary phase in chromatographic experiments to assess the functional activity of series of noncompetitive inhibitors (NCIs) as reflected in their IC50 values. Chromatographically determined retention values and computer generated molecular descriptors were obtained for 29 compounds and the data were analyzed by cluster analysis. The approach qualitatively ranked the test compounds as efficient NCIs (low IC50 values) or poor NCIs (high IC50 values). The data obtained with the 29 compounds used in this study demonstrate that the experimental approach had been able to place 25 of these compounds in the correct IC(50) clusters. To our knowledge, this is the first relationship established between chromatographic retention and IC50 for membrane-bound receptors. These results suggest that the chromatographic approach may be useful in development of lead drug candidates including the determination of off-target binding. JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences AU - Jozwiak, Krzysztof AU - Moaddel, Ruin AU - Yamaguchi, Rika AU - Ravichandran, Sarangan AU - Collins, Jack R AU - Wainer, Irving W AD - Gerontology Research Center, National institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. krzysztof.jozwiak@am.lublin.pl Y1 - 2005/05/05/ PY - 2005 DA - 2005 May 05 SP - 169 EP - 174 VL - 819 IS - 1 SN - 1570-0232, 1570-0232 KW - Nicotinic Antagonists KW - 0 KW - Receptors, Nicotinic KW - Index Medicus KW - Thermodynamics KW - Cluster Analysis KW - Multivariate Analysis KW - Nicotinic Antagonists -- pharmacology KW - Inhibitory Concentration 50 KW - Nicotinic Antagonists -- analysis KW - Chromatography, Affinity -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67564153?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+chromatography.+B%2C+Analytical+technologies+in+the+biomedical+and+life+sciences&rft.atitle=Qualitative+assessment+of+IC50+values+of+inhibitors+of+the+neuronal+nicotinic+acetylcholine+receptor+using+a+single+chromatographic+experiment+and+multivariate+cluster+analysis.&rft.au=Jozwiak%2C+Krzysztof%3BMoaddel%2C+Ruin%3BYamaguchi%2C+Rika%3BRavichandran%2C+Sarangan%3BCollins%2C+Jack+R%3BWainer%2C+Irving+W&rft.aulast=Jozwiak&rft.aufirst=Krzysztof&rft.date=2005-05-05&rft.volume=819&rft.issue=1&rft.spage=169&rft.isbn=&rft.btitle=&rft.title=Journal+of+chromatography.+B%2C+Analytical+technologies+in+the+biomedical+and+life+sciences&rft.issn=15700232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-03-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The anti-apoptotic, glucocorticoid receptor cochaperone protein BAG-1 is a long-term target for the actions of mood stabilizers. AN - 67798275; 15872096 AB - Increasing data suggest that impairments of cellular plasticity/resilience underlie the pathophysiology of bipolar disorder. A series of microarray studies with validating criteria have recently revealed a common, novel target for the long-term actions of the structurally highly dissimilar mood stabilizers lithium and valproate: BAG-1 [BCL-2 (B-cell CLL/lymphoma 2)-associated athanogene]. Because BAG-1 attenuates glucocorticoid receptor (GR) nuclear translocation, activates ERK (extracellular signal-regulated kinase) MAP (mitogen-activated protein) kinases, and potentiates anti-apoptotic functions of BCL-2, extensive additional studies were undertaken. Chronic administration of both agents at therapeutic doses increased the expression of BAG-1 in rat hippocampus. Furthermore, these findings were validated at the protein level, and the effects were seen in a time frame consistent with therapeutic effects and were specific for mood stabilizers. Functional studies showed that either lithium or valproate, at therapeutically relevant levels, inhibited dexamethasone-induced GR nuclear translocation and inhibited GR transcriptional activity. Furthermore, small interfering RNA studies showed that these inhibitory effects on GR activity were mediated, at least in part, through BAG-1. The observation that BAG-1 inhibits glucocorticoid activation suggests that mood stabilizers may counteract the deleterious effects of hypercortisolemia seen in bipolar disorder by upregulating BAG-1. Additionally, these studies suggest that regulation of GR-mediated plasticity may play a role in the treatment of bipolar disorder and raise the possibility that agents affecting BAG-1 more directly may represent novel therapies for this devastating illness. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Zhou, Rulun AU - Gray, Neil A AU - Yuan, Peixiong AU - Li, Xiaoxia AU - Chen, Jingshan AU - Chen, Guang AU - Damschroder-Williams, Patricia AU - Du, Jing AU - Zhang, Lei AU - Manji, Husseini K AD - Laboratory of Molecular Pathophysiology, National Institute of Mental Health, Bethesda, Maryland 20852, USA. Y1 - 2005/05/04/ PY - 2005 DA - 2005 May 04 SP - 4493 EP - 4502 VL - 25 IS - 18 KW - Antimanic Agents KW - 0 KW - BCL2-associated athanogene 1 protein KW - DNA-Binding Proteins KW - Indoles KW - RNA, Small Interfering KW - Receptors, Glucocorticoid KW - Transcription Factors KW - DAPI KW - 47165-04-8 KW - Valproic Acid KW - 614OI1Z5WI KW - Dexamethasone KW - 7S5I7G3JQL KW - Lithium KW - 9FN79X2M3F KW - Alkaline Phosphatase KW - EC 3.1.3.1 KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Dexamethasone -- pharmacology KW - Humans KW - Alkaline Phosphatase -- metabolism KW - Blotting, Western -- methods KW - Cell Line, Tumor KW - Immunohistochemistry -- methods KW - Alkaline Phosphatase -- genetics KW - Receptors, Glucocorticoid -- metabolism KW - Neuroblastoma KW - Molecular Weight KW - Behavior, Animal KW - Rats KW - Transfection -- methods KW - Rats, Wistar KW - RNA, Small Interfering -- pharmacology KW - Indoles -- metabolism KW - Time Factors KW - Male KW - Valproic Acid -- pharmacology KW - Antimanic Agents -- pharmacology KW - Transcription Factors -- metabolism KW - Hippocampus -- metabolism KW - DNA-Binding Proteins -- genetics KW - Transcription Factors -- genetics KW - Lithium -- pharmacology KW - Hippocampus -- drug effects KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67798275?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=The+anti-apoptotic%2C+glucocorticoid+receptor+cochaperone+protein+BAG-1+is+a+long-term+target+for+the+actions+of+mood+stabilizers.&rft.au=Zhou%2C+Rulun%3BGray%2C+Neil+A%3BYuan%2C+Peixiong%3BLi%2C+Xiaoxia%3BChen%2C+Jingshan%3BChen%2C+Guang%3BDamschroder-Williams%2C+Patricia%3BDu%2C+Jing%3BZhang%2C+Lei%3BManji%2C+Husseini+K&rft.aulast=Zhou&rft.aufirst=Rulun&rft.date=2005-05-04&rft.volume=25&rft.issue=18&rft.spage=4493&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-23 N1 - Date created - 2005-05-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functionally distinct polymorphic sequences in the human genome that are targets for p53 transactivation. AN - 67800075; 15843459 AB - The p53 tumor suppressor protein is a master regulatory transcription factor that coordinates cellular responses to DNA damage and cellular stress. Besides mutations in p53, or in proteins involved in the p53 response pathway, genetic variation in promoter response elements (REs) of p53 target genes is expected to alter biological responses to stress. To identify SNPs in p53 REs that may modify p53-controlled gene expression, we developed an approach that combines a custom bioinformatics search to identify candidate SNPs with functional yeast and mammalian cell assays to assess their effect on p53 transactivation. Among approximately 2 million human SNPs, we identified >200 that seem to disrupt functional p53 REs. Eight of these SNPs were evaluated in functional assays to determine both the activity of the putative RE and the impact of the candidate SNPs on transactivation. All eight candidate REs were functional, and in every case the SNP pair exhibited differential transactivation capacities. Additionally, six of the eight genes adjacent to these SNPs are induced by genotoxic stress or are activated directly by transfection with p53 cDNA. Thus, this strategy efficiently identifies SNPs that may differentially affect gene expression responses in the p53 regulatory pathway. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Tomso, Daniel J AU - Inga, Alberto AU - Menendez, Daniel AU - Pittman, Gary S AU - Campbell, Michelle R AU - Storici, Francesca AU - Bell, Douglas A AU - Resnick, Michael A AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Y1 - 2005/05/03/ PY - 2005 DA - 2005 May 03 SP - 6431 EP - 6436 VL - 102 IS - 18 SN - 0027-8424, 0027-8424 KW - DNA, Complementary KW - 0 KW - Tumor Suppressor Protein p53 KW - Luciferases KW - EC 1.13.12.- KW - Index Medicus KW - Yeasts KW - DNA, Complementary -- genetics KW - Plasmids -- genetics KW - Humans KW - Cell Line, Tumor KW - Computational Biology KW - Reverse Transcriptase Polymerase Chain Reaction KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Sequence Alignment KW - Transfection KW - Promoter Regions, Genetic -- genetics KW - Response Elements -- genetics KW - Polymorphism, Single Nucleotide -- genetics KW - Genome, Human KW - Transcriptional Activation -- genetics KW - Gene Expression Regulation KW - Tumor Suppressor Protein p53 -- genetics KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67800075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Functionally+distinct+polymorphic+sequences+in+the+human+genome+that+are+targets+for+p53+transactivation.&rft.au=Tomso%2C+Daniel+J%3BInga%2C+Alberto%3BMenendez%2C+Daniel%3BPittman%2C+Gary+S%3BCampbell%2C+Michelle+R%3BStorici%2C+Francesca%3BBell%2C+Douglas+A%3BResnick%2C+Michael+A&rft.aulast=Tomso&rft.aufirst=Daniel&rft.date=2005-05-03&rft.volume=102&rft.issue=18&rft.spage=6431&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cell. 2000 Sep 15;102(6):849-62 [11030628] Nucleic Acids Res. 2001 Jan 1;29(1):308-11 [11125122] Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3416-21 [11248093] Nat Genet. 2001 Apr;27(4):371-2 [11279516] Arthritis Rheum. 2001 Aug;44(8):1782-5 [11508429] Mol Cell. 2001 Jul;8(1):57-69 [11511360] Biochemistry. 2002 May 28;41(21):6714-22 [12022875] Hum Mutat. 2002 Jun;19(6):607-14 [12007217] Annu Rev Biochem. 2002;71:817-46 [12045112] Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8467-72 [12077306] Genes Dev. 1999 Nov 15;13(22):3027-33 [10580009] Genes Dev. 2000 Apr 15;14(8):981-93 [10783169] Oncogene. 2002 Nov 7;21(51):7901-11 [12420228] Mol Cell Biol. 2002 Dec;22(24):8612-25 [12446780] J Immunol. 2003 Jan 1;170(1):132-8 [12496392] Nat Genet. 2003 Apr;33(4):457-8 [12652301] Nat Genet. 2003 Apr;33(4):439-40 [12665861] Cancer Lett. 2003 Apr 10;193(1):99-107 [12691829] Environ Health Perspect. 2003 Jun;111(8):1055-64 [12826477] Gene. 2003 Jul 17;312:207-13 [12909357] Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9934-9 [12909720] Mol Cell. 2003 Oct;12(4):1015-27 [14580351] Science. 2003 Nov 7;302(5647):1041-3 [14605368] Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9 [14630945] Nucleic Acids Res. 2004 Jan 1;32(Database issue):D528-32 [14681474] Physiol Genomics. 2004 Jan 15;16(2):184-93 [14583597] Cell. 2004 Feb 20;116(4):499-509 [14980218] Science. 2004 Mar 5;303(5663):1526-9 [14976262] Cancer Res. 2004 May 15;64(10):3361-4 [15150084] Science. 1992 May 8;256(5058):827-30 [1589764] Nat Genet. 1992 Apr;1(1):45-9 [1301998] J Natl Cancer Inst. 1993 Jul 21;85(14):1159-64 [8320745] Hum Mol Genet. 1994 Sep;3(9):1537-42 [7833908] Genes Dev. 1996 May 1;10(9):1054-72 [8654922] Brain Res Dev Brain Res. 1999 Jun 2;115(2):183-93 [10407135] Cell. 2004 Nov 24;119(5):591-602 [15550242] Nature. 2000 Nov 16;408(6810):307-10 [11099028] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of a UDP-glucuronosyltransferase regulates substrate specificity. AN - 67798628; 15845768 AB - UDP-glucuronosyltransferase (UGT) isozymes catalyze detoxification of numerous chemical toxins present in our daily diet and environment by conjugation to glucuronic acid. The special properties and enzymatic mechanism(s) that enable endoplasmic reticulum-bound UGT isozymes to convert innumerable structurally diverse lipophiles to excretable glucuronides are unknown. Inhibition of cellular UGT1A7 and UGT1A10 activities and of [33P]orthophosphate incorporation into immunoprecipitable proteins after exposure to curcumin or calphostin-C indicated that the isozymes are phosphorylated. Furthermore, inhibition of UGT phosphorylation and activity by treatment with PKCepsilon-specific inhibitor peptide supported PKC involvement. Co-immunoprecipitation, colocalization by means of immunofluorescence, and cross-linking studies of PKCepsilon and UGT1A7His revealed that the proteins reside within 11.4 angstroms of each other. Moreover, mutation of three PKC sites in each UGT isozyme demonstrated that T73A/G and T202A/G caused null activity, whereas S432G-UGT1A7 caused a major shift of its pH-8.5 optimum to 6.4 with new substrate selections, including 17beta-estradiol. S432G-UGT1A10 exhibited a minor pH shift without substrate alterations. PKCepsilon involvement was confirmed by the demonstration that PKCepsilon overexpression enhanced activity of UGT1A7 but not of its S432 mutant and the conversion of 17beta-[14C]estradiol by S432G-UGT1A7 but not by UGT1A7. Consistent with these observations, treatment of UGT1A7-transfected cells with PKCepsilon-specific inhibitor peptide or general PKC inhibitors increased 17beta-estradiol catalysis between 5- and 11-fold, with parallel decreases in phosphoserine-432. Here, we report a mechanism involving PKC-mediated phosphorylation of UGT such that phosphoserine/threonine regulates substrate specificity in response to chemical exposures, which possibly confers survival benefit. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Basu, Nikhil K AU - Kovarova, Martina AU - Garza, Amanda AU - Kubota, Shigeki AU - Saha, Tapas AU - Mitra, Partha S AU - Banerjee, Rajat AU - Rivera, Juan AU - Owens, Ida S AD - Heritable Disorders Branch, National Institute of Child Health and Human Development, National Institutes of Health, Building 10, Room 9S-241, Bethesda, MD 20892-1830, USA. Y1 - 2005/05/03/ PY - 2005 DA - 2005 May 03 SP - 6285 EP - 6290 VL - 102 IS - 18 SN - 0027-8424, 0027-8424 KW - Carbon Radioisotopes KW - 0 KW - Isoenzymes KW - Naphthalenes KW - Phosphoserine KW - 17885-08-4 KW - Threonine KW - 2ZD004190S KW - Estradiol KW - 4TI98Z838E KW - Glucuronosyltransferase KW - EC 2.4.1.17 KW - UDP-glucuronosyltransferase, UGT1A7 KW - Protein Kinase C KW - EC 2.7.11.13 KW - calphostin C KW - I271P23G24 KW - Curcumin KW - IT942ZTH98 KW - Index Medicus KW - Animals KW - Threonine -- metabolism KW - COS Cells KW - Humans KW - Hydrogen-Ion Concentration KW - Immunoprecipitation KW - Cell Line, Tumor KW - Phosphoserine -- metabolism KW - Mutagenesis KW - Estradiol -- metabolism KW - Naphthalenes -- metabolism KW - Blotting, Western KW - Curcumin -- metabolism KW - Phosphorylation KW - Transfection KW - Cercopithecus aethiops KW - Mutation -- genetics KW - Substrate Specificity KW - Fluorescent Antibody Technique KW - Protein Kinase C -- metabolism KW - Protein Kinase C -- antagonists & inhibitors KW - Glucuronosyltransferase -- genetics KW - Glucuronosyltransferase -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67798628?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Protocol+optimization+for+the+evaluation+of+in+vitro+cytotoxicity+assays+for+estimating+rodent+and+human+acute+systemic+toxicity&rft.au=Paris%2C+M%3BStrickland%2C+J%3BStokes%2C+W%3BCasati%2C+S%3BTice%2C+R%3BRaabe%2C+H%3BCao%2C+C%3BClothier%2C+R%3BHaseman%2C+J%3BCrockett%2C+P&rft.aulast=Paris&rft.aufirst=M&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Biochem Biophys Res Commun. 2001 Jul 27;285(4):997-1006 [11467851] Biochem Biophys Res Commun. 1999 Jun 24;260(1):199-202 [10381366] Biochem Biophys Res Commun. 2003 Mar 28;303(1):98-104 [12646172] Cancer Res. 2003 Aug 15;63(16):5118-25 [12941843] J Biol Chem. 2004 Jan 9;279(2):1429-41 [14557274] Drug Metab Dispos. 2004 Jul;32(7):768-73 [15205394] J Biol Chem. 2004 Jul 2;279(27):28320-9 [15117964] J Biol Chem. 1972 Apr 25;247(8):2558-65 [4336378] Hepatology. 1989 Aug;10(2):163-7 [2501210] J Biol Chem. 1991 Jan 15;266(2):1043-7 [1898728] J Clin Invest. 1992 Jul;90(1):150-5 [1634606] J Clin Invest. 1992 Sep;90(3):799-809 [1522235] Carcinogenesis. 1993 May;14(5):857-61 [8504477] J Biol Chem. 1994 Jun 17;269(24):16862-6 [8207009] J Biol Chem. 1995 Feb 17;270(7):3284-91 [7852413] Biochemistry. 2000 Mar 7;39(9):2269-75 [10694393] FASEB J. 2001 Jul;15(9):1613-5 [11427503] Cancer Res. 1995 Mar 1;55(5):1045-51 [7866987] Science. 1995 Apr 14;268(5208):247-51 [7716516] Biochemistry. 1996 Aug 6;35(31):10119-24 [8756475] J Biol Chem. 1997 Jan 17;272(3):1417-20 [8999804] J Immunol. 1997 Sep 15;159(6):2624-32 [9300681] Exp Cell Res. 1998 Jul 10;242(1):294-302 [9665827] Cell Growth Differ. 1999 Mar;10(3):183-91 [10099832] Eur J Biochem. 1999 Mar;260(3):785-93 [10103008] Biochem J. 2002 May 1;363(Pt 3):537-45 [11964154] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Suppression of gross chromosomal rearrangements by the multiple functions of the Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae. AN - 67712680; 15811632 AB - The Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae has roles in the intra-S checkpoint, homologous recombination, non-homologous end joining, meiotic recombination, telomere maintenance and the suppression of gross chromosomal rearrangements (GCRs). The discovery of mutations in the genes encoding the human homologues of two MRX subunits that underlie the chromosome fragility syndromes, Ataxia telangiectasia-like disorder and Nijmegen breakage syndrome suggest that the MRX complex also functions in suppression of GCRs in human cells. Previously, we demonstrated that the deletion mutations in each of the MRX genes increased the rate of GCRs up to 1000-fold compared to wild-type rates. However, it has not been clear which molecular function of the MRX complex is important for suppression of GCRs. Here, we present evidence that at least three different activities of the MRX complex are important for suppression of GCRs. These include the nuclease activity of Mre11, an activity related to MRX complex formation and another activity that has a close link with the telomere maintenance function of the MRX complex. An activity related to MRX complex formation is especially important for the suppression of translocation type of GCRs. However, the non-homologous end joining function of MRX complex does not appear to participate in the suppression of GCRs. JF - DNA repair AU - Smith, Stephanie AU - Gupta, Amitabha AU - Kolodner, Richard D AU - Myung, Kyungjae AD - Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA. Y1 - 2005/05/02/ PY - 2005 DA - 2005 May 02 SP - 606 EP - 617 VL - 4 IS - 5 SN - 1568-7864, 1568-7864 KW - Saccharomyces cerevisiae Proteins KW - 0 KW - XRS2 protein, S cerevisiae KW - Endodeoxyribonucleases KW - EC 3.1.- KW - Exodeoxyribonucleases KW - MRE11 protein, S cerevisiae KW - Adenosine Triphosphatases KW - EC 3.6.1.- KW - RAD5 protein, S cerevisiae KW - DNA Helicases KW - EC 3.6.4.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Telomere -- genetics KW - Mutation -- genetics KW - Translocation, Genetic KW - Saccharomyces cerevisiae -- genetics KW - Saccharomyces cerevisiae Proteins -- metabolism KW - Saccharomyces cerevisiae -- metabolism KW - Saccharomyces cerevisiae Proteins -- genetics KW - Adenosine Triphosphatases -- metabolism KW - Gene Rearrangement KW - Endodeoxyribonucleases -- genetics KW - Endodeoxyribonucleases -- metabolism KW - Exodeoxyribonucleases -- genetics KW - Exodeoxyribonucleases -- metabolism KW - Adenosine Triphosphatases -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67712680?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=DNA+repair&rft.atitle=Suppression+of+gross+chromosomal+rearrangements+by+the+multiple+functions+of+the+Mre11-Rad50-Xrs2+complex+in+Saccharomyces+cerevisiae.&rft.au=Smith%2C+Stephanie%3BGupta%2C+Amitabha%3BKolodner%2C+Richard+D%3BMyung%2C+Kyungjae&rft.aulast=Smith&rft.aufirst=Stephanie&rft.date=2005-05-02&rft.volume=4&rft.issue=5&rft.spage=606&rft.isbn=&rft.btitle=&rft.title=DNA+repair&rft.issn=15687864&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-04-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of urethane-induced genotoxicity and cell proliferation in CYP2E1-null mice AN - 17503121; 6391163 AB - Urethane is a multi-site animal carcinogen and was classified as ''reasonably anticipated to be a human carcinogen.'' Urethane is a fermentation by-product and found at appreciable levels in alcoholic beverages and foods such as bread and cheese. Recent work in this laboratory demonstrated for the first time that CYP2E1 is the principal enzyme responsible for urethane metabolism. The current studies were undertaken to assess the relationships between CYP2E1-mediated metabolism and urethane-induced genotoxicity and cell proliferation as determined by induction of micronucleated erythrocytes (MN) and expression of Ki-67, respectively, using CYP2E1-null and wild-type mice. Urethane was administered at 0 (vehicle), 1, 10, or 100mg/kg/day (p.o.), 5 days/week for 6 weeks. A significant dose-dependent increase in MN was observed in wild-type mice; however, a slight increase was measured in the MN-polychromatic erythrocytes in CYP2E1-null mice treated with 100mg/kg. A significant increase in the expression of Ki-67 was detected in the livers and the lungs (terminal bronchioles, alveoli, and bronchi) of wild-type mice administered 100mg urethane /kg in comparison to controls. In contrast, CYP2E1-null mice administered this dose exhibited negligible alterations in Ki-67 expression in the livers and lungs compared to controls. Interestingly, while Ki-67 expression in the forestomach decreased in wild-type mice, it increased in CYP2E1-null mice. Subsequent comparative metabolism studies demonstrated that total urethane-derived radioactivity in the plasma, liver, and lung was significantly higher in CYP2E1-null versus wild-type mice and un-metabolized urethane constituted greater than 83% of the radioactivity in CYP2E1-null mice. Un-metabolized urethane was not detectable in the plasma, liver, and lung of wild-type mice. In conclusion, these data demonstrated that CYP2E1-mediated metabolism of urethane, presumably via epoxide formation, is necessary for the induction of genotoxicity, and cell proliferation in the liver and lung of wild-type mice. JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis AU - Hoffler, U AU - Dixon, D AU - Peddada, S AU - Ghanayem, B I AD - Meharry Medical College, Nashville, TN, USA, ghanayem@niehs.nih.gov Y1 - 2005/05/02/ PY - 2005 DA - 2005 May 02 SP - 58 EP - 72 PB - Elsevier B.V. VL - 572 IS - 1-2 SN - 0027-5107, 0027-5107 KW - Toxicology Abstracts KW - Bread KW - Epoxides KW - Alcoholic beverages KW - Fermentation KW - Erythrocytes KW - Genotoxicity KW - Carcinogens KW - Cheese KW - Alveoli KW - Bronchus KW - Lung KW - Liver KW - Radioactivity KW - Cell proliferation KW - Manganese KW - urethane KW - Metabolism KW - X 24221:Toxicity testing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17503121?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.atitle=Inhibition+of+urethane-induced+genotoxicity+and+cell+proliferation+in+CYP2E1-null+mice&rft.au=Hoffler%2C+U%3BDixon%2C+D%3BPeddada%2C+S%3BGhanayem%2C+B+I&rft.aulast=Hoffler&rft.aufirst=U&rft.date=2005-05-02&rft.volume=572&rft.issue=1-2&rft.spage=58&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.issn=00275107&rft_id=info:doi/10.1016%2Fj.mrfmmm.2004.12.005 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - urethane; Lung; Liver; Metabolism; Cell proliferation; Genotoxicity; Radioactivity; Erythrocytes; Carcinogens; Manganese; Alcoholic beverages; Bronchus; Epoxides; Alveoli; Fermentation; Bread; Cheese DO - http://dx.doi.org/10.1016/j.mrfmmm.2004.12.005 ER - TY - JOUR T1 - Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro AN - 954577441; 13860132 AB - With the advent of recent protocols to isolate multipotent human mesenchymal stem cells (MSCs), there is a need for efficient transfection methodologies for these cells. Most standard transfection methods yield poor transfection efficiencies for MSCs (<1%). Here we have optimized a high-efficiency transfection technique for low passage MSCs derived from adult human bone marrow. This technique is an extension of electroporation, termed amaxa Nucleofection(TM), where plasmid DNA is transfected directly into the cell nucleus, independent of the growth state of the cell. With this technique, we demonstrate up to 90% transfection efficiency of the viable population of MSCs, using plasmid construct containing a standard cytomegalovirus (CMV) early promoter driving expression of green fluorescent protein (GFP). Although little variation in transfection efficiency was observed between patient samples, a 2-fold difference in transfection efficiency and a 10-fold difference in expression levels per cell were seen using two distinct CMV-GFP expression plasmids. By fluorescence-activated cell sorting, the GFP expressing cells were sorted and subcultured. At 2 wk posttransfection, approx 25% of the population of sorted cells were GFP positive, and by 3 wk, nearly 10% of the cells still retained GFP expression. Transfection of these cells with plasmid containing either the collagen type I (Colla1) promoter or the cartilage oligomeric matrix protein (COMP) promoter, each driving expression of GFP, produced a somewhat lower transfection efficiency (approx 40%), due in part to the lower activity of transcription from these promoters compared to that of CMV. Transfection with the collagen type II (Col2a1) promoter linked to GFP exhibited low expression, due to the fact that collagen type II is not expressed in these cells. Upon culturing of the Col2a1-GFP transfected cells in a transforming growth factor-b3-containing medium known to induce mesenchymal chondrogensis, a significant enhancement of GFP level was seen, indicating the ability of the transfected cells to differentiate into chondrocytes and express cartilage-specific genes, such as Col2a1. Taken together, these data provide evidence of the applicability of this technique for the efficient transfection of MSCs. JF - Molecular Biotechnology AU - Haleem-Smith, Hana AU - Derfoul, Assia AU - Okafor, Chukwuka AU - Tuli, Richard AU - Olsen, Douglas AU - Hall, David J AU - Tuan, Rocky S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Drive, Rm 1503, 20892-8022, Bethesda, MD, USA, tuanr@mail.nih.gov. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 9 EP - 19 PB - Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA VL - 30 IS - 1 SN - 1073-6085, 1073-6085 KW - Biotechnology and Bioengineering Abstracts KW - Cartilage oligomeric matrix protein KW - Data processing KW - Electroporation KW - Bone marrow KW - Green fluorescent protein KW - Chondrocytes KW - Transcription KW - Plasmids KW - Cytomegalovirus KW - Flow cytometry KW - Promoters KW - Stem cells KW - Transfection KW - DNA KW - Collagen (type II) KW - Mesenchyme KW - Nuclei KW - Collagen (type I) KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/954577441?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Biotechnology&rft.atitle=Optimization+of+high-efficiency+transfection+of+adult+human+mesenchymal+stem+cells+in+vitro&rft.au=Haleem-Smith%2C+Hana%3BDerfoul%2C+Assia%3BOkafor%2C+Chukwuka%3BTuli%2C+Richard%3BOlsen%2C+Douglas%3BHall%2C+David+J%3BTuan%2C+Rocky+S&rft.aulast=Haleem-Smith&rft.aufirst=Hana&rft.date=2005-05-01&rft.volume=30&rft.issue=1&rft.spage=9&rft.isbn=&rft.btitle=&rft.title=Molecular+Biotechnology&rft.issn=10736085&rft_id=info:doi/10.1385%2FMB%3A30%3A1%3A009 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2012-03-01 N1 - Last updated - 2012-03-30 N1 - SubjectsTermNotLitGenreText - Cartilage oligomeric matrix protein; Data processing; Electroporation; Green fluorescent protein; Bone marrow; Transcription; Chondrocytes; Plasmids; Flow cytometry; Promoters; Stem cells; Transfection; DNA; Collagen (type II); Nuclei; Mesenchyme; Collagen (type I); Cytomegalovirus DO - http://dx.doi.org/10.1385/MB:30:1:009 ER - TY - JOUR T1 - Task-dependent intracortical inhibition is impaired in focal hand dystonia. AN - 85385313; pmid-15641012 AB - We tested whether task-dependent modulation of inhibition within the motor cortex is impaired in patients with dystonia. Paired-pulse transcranial magnetic stimulation (TMS) at an interstimulus interval of 2 msec was used to measure the effect of two different tasks on short ISI intracortical inhibition (SICI) in dystonic and normal subjects. In two experiments, SICI of the fourth dorsal interosseus (4DIO) and abductor pollicis brevis (APB) muscles were measured before and at the end of the training task. In the first experiment, subjects performed a nonselective task consisting of abducting the thumb, where the APB acted as agonist and the 4DIO as synergist. In the second experiment, the function of the 4DIO was changed as the subjects were asked to consciously inhibit this muscle while abducting the thumb (selective task). Therefore, while the APB was activated in both tasks, the 4DIO was activated in the nonselective task but was in the inhibitory surround in the selective task. We found that performance of the selective but not the nonselective task resulted in increased SICI in the 4DIO of normal but not in dystonic subjects. We conclude that task-dependent SICI is disturbed in patients with dystonia.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Bütefisch, Cathrin M AU - Boroojerdi, Babak AU - Chen, Robert AU - Battaglia, Fortunato AU - Hallett, Mark AD - Human Motor Control Section, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland 20892-1428, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 545 EP - 551 VL - 20 IS - 5 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Dystonic Disorders: diagnosis KW - *Dystonic Disorders: physiopathology KW - Dystonic Disorders: therapy KW - Electromyography KW - Feedback KW - Female KW - Functional Laterality: physiology KW - *Hand: physiopathology KW - Humans KW - Magnetics: instrumentation KW - Male KW - Middle Aged KW - *Neural Inhibition: physiology KW - *Neural Pathways: physiopathology KW - *Psychomotor Performance KW - Severity of Illness Index KW - Teaching: methods KW - Visual Perception UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85385313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Task-dependent+intracortical+inhibition+is+impaired+in+focal+hand+dystonia.&rft.au=B%C3%BCtefisch%2C+Cathrin+M%3BBoroojerdi%2C+Babak%3BChen%2C+Robert%3BBattaglia%2C+Fortunato%3BHallett%2C+Mark&rft.aulast=B%C3%BCtefisch&rft.aufirst=Cathrin&rft.date=2005-05-01&rft.volume=20&rft.issue=5&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2012-03-15 N1 - SuppNotes - Cites: J Neurol Neurosurg Psychiatry. 1989 Sep;52(9):1043-9[2795073]; Cites: Neurology. 1989 Jan;39(1):85-9[2909917]; Cites: Proc Natl Acad Sci U S A. 1988 Mar;85(6):2003-7[3162322]; Cites: Mov Disord. 1988;3(1):61-9[3173365]; Cites: Neurology. 1988 Jul;38(7):1005-12[3386815]; Cites: J Neurol Neurosurg Psychiatry. 1985 Aug;48(8):782-7[4031930]; Cites: Brain. 1985 Sep;108 ( Pt 3):593-608[4041776]; Cites: Electroencephalogr Clin Neurophysiol. 1994 Apr;93(2):138-46[7512920]; Cites: Electroencephalogr Clin Neurophysiol. 1994 Aug;91(2):79-92[7519144]; Cites: Ann Neurol. 1995 Feb;37(2):181-8[7847860]; Cites: Brain. 1994 Aug;117 ( Pt 4):859-76[7922471]; Cites: J Physiol. 1993 Nov;471:501-19[8120818]; Cites: J Neurol Neurosurg Psychiatry. 1995 Nov;59(5):493-8[8530933]; Cites: Mov Disord. 1995 Sep;10(5):556-61[8552105]; Cites: Exp Brain Res. 1996 Apr;109(1):127-35[8740215]; Cites: Ann Neurol. 1996 Sep;40(3):367-78[8797526]; Cites: Electroencephalogr Clin Neurophysiol. 1996 Dec;101(6):478-82[9020819]; Cites: J Physiol. 1997 Feb 1;498 ( Pt 3):817-23[9051592]; Cites: Neurosci Lett. 1997 Feb 7;222(3):167-70[9148241]; Cites: J Neurol Neurosurg Psychiatry. 1985 Jul;48(7):650-7[4031909]; Cites: Neurology. 1997 Oct;49(4):1054-9[9339689]; Cites: Exp Brain Res. 1998 Feb;118(3):421-6[9497149]; Cites: Exp Brain Res. 1998 Mar;119(2):265-8[9535577]; Cites: Brain. 1998 Jul;121 ( Pt 7):1195-212[9679773]; Cites: Adv Neurol. 1998;78:11-8[9750898]; Cites: Clin Neurophysiol. 1999 Mar;110(3):550-5[10363778]; Cites: Exp Brain Res. 2002 Sep;146(1):86-94[12192582]; Cites: J Neurol Sci. 1992 Nov;113(1):85-90[1469459]; Cites: J Neurophysiol. 1991 Jun;65(6):1542-53[1875261]; Cites: Neurology. 1991 Sep;41(9):1449-56[1891097]; Cites: J Neurophysiol. 1989 Apr;61(4):747-58[2542471]; Cites: Brain. 1989 Jun;112 ( Pt 3):681-97[2731027] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - The effects of tones on speaking frequency and intensity ranges in Mandarin and Min dialects. AN - 85382418; pmid-15957789 AB - The differences of speaking frequency and intensity in different tonal dialects has not been widely investigated. The purposes of this study were (1) to compare the speaking frequency and speaking intensity ranges of Mandarin and Min and (2) to compare the speaking frequency and intensity ranges of Mandarin and Min to those of American English. The subjects were 80 normal Taiwanese adults divided into two dialect groups, Mandarin and Min. The speaking F0, the highest speaking F0, the lowest speaking F0, the maximum range of speaking F0, and the intensity counterpart were obtained from reading in their native dialects. Statistical analysis revealed that Min speakers had a significantly greater maximum range of speaking intensity and a smaller lowest speaking intensity than Mandarin speakers, which indicated tonal effects by speakers of the Min dialect. Moreover, Mandarin and Min speakers had a greater maximum range of speaking F0 and maximum range of speaking intensity than American English speakers. The data may provide an assessment tool for Mandarin speakers and Min speakers. JF - The Journal of the Acoustical Society of America AU - Chen, Sheng H AD - Department of Speech and Hearing Disorders and Sciences, National Taipei College of Nursing, #89, Nei-Chiang Street, Wanhua 108, Taipei, Taiwan. shchen@ntcn.edu.tw Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3225 EP - 3230 VL - 117 IS - 5 SN - 0001-4966, 0001-4966 KW - Index Medicus KW - National Library of Medicine KW - Adult KW - China KW - Female KW - Humans KW - *Language KW - Male KW - *Phonation: physiology KW - *Pitch Perception KW - *Speech: physiology KW - Speech Production Measurement KW - Time Factors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85382418?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+the+Acoustical+Society+of+America&rft.atitle=The+effects+of+tones+on+speaking+frequency+and+intensity+ranges+in+Mandarin+and+Min+dialects.&rft.au=Chen%2C+Sheng+H&rft.aulast=Chen&rft.aufirst=Sheng&rft.date=2005-05-01&rft.volume=117&rft.issue=5&rft.spage=3225&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+the+Acoustical+Society+of+America&rft.issn=00014966&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Investigation of the role of congenital cytomegalovirus infection in the etiology of enlarged vestibular aqueducts. AN - 85379421; pmid-15897416 AB - To determine whether congenital cytomegalovirus (CMV) infection is an etiologic factor in the pathogenesis of enlarged vestibular aqueducts (EVA).Two different cohort studies. Subjects The study population comprised 19 subjects with a history of congenital CMV infection and sensorineural hearing loss (cohort 1); 39 subjects with nonsyndromic EVA and their unaffected mothers (cohort 2); and 16 control subjects with EVA associated with Pendred syndrome and bi-allelic mutations of the SLC26A4 gene and their unaffected mothers.In cohort 1, we detected EVA in 0 of 19 subjects with congenital CMV infection and sensorineural hearing loss. In cohort 2, anti-CMV serologic profiles were consistent with possible congenital CMV infection in 10 (26%) of 39 subjects with nonsyndromic EVA and 6 (38%) of 16 control subjects with Pendred syndrome (P = .52). These seroprevalence rates are similar to those expected in the general population (40%).In spite of their auditory phenotypic similarities, congenital CMV infection is not a significant factor in the etiology of EVA. JF - Archives of otolaryngology--head & neck surgery AU - Pryor, Shannon P AU - Demmler, Gail J AU - Madeo, Anne C AU - Yang, Yandan AU - Zalewski, Chris K AU - Brewer, Carmen C AU - Butman, John A AU - Fowler, Karen B AU - Griffith, Andrew J AD - Hearing Section, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 388 EP - 392 VL - 131 IS - 5 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - Adolescent KW - Audiometry KW - Child KW - Child, Preschool KW - Cohort Studies KW - *Cytomegalovirus Infections: complications KW - *Cytomegalovirus Infections: congenital KW - Cytomegalovirus Infections: genetics KW - Female KW - Hearing Loss, Sensorineural: virology KW - Humans KW - Infant KW - Magnetic Resonance Imaging KW - Male KW - Mutation KW - *Vestibular Aqueduct: virology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85379421?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Investigation+of+the+role+of+congenital+cytomegalovirus+infection+in+the+etiology+of+enlarged+vestibular+aqueducts.&rft.au=Pryor%2C+Shannon+P%3BDemmler%2C+Gail+J%3BMadeo%2C+Anne+C%3BYang%2C+Yandan%3BZalewski%2C+Chris+K%3BBrewer%2C+Carmen+C%3BButman%2C+John+A%3BFowler%2C+Karen+B%3BGriffith%2C+Andrew+J&rft.aulast=Pryor&rft.aufirst=Shannon&rft.date=2005-05-01&rft.volume=131&rft.issue=5&rft.spage=388&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Neurobehavioral effects of harmful algal bloom (HAB) toxins: a critical review. AN - 85378910; pmid-15892909 AB - Human exposure to naturally occurring marine toxins has been associated with a range of neurobehavioral abnormalities. The toxins are produced by harmful algal blooms (HABs) and are typically contracted through seafood consumption. The primary target of many of the HAB toxins is the neurologic system, and the neurobehavioral symptoms associated with the HAB illnesses have influenced public health policy. The HAB-related illnesses most frequently linked to neuropsychological disturbance are Amnesic Shellfish Poisoning, Ciguatera Fish Poisoning, and Possible Estuarine Associated Syndrome, which is associated with exposure to the Pfiesteria piscicida organism. Although the neurophysiologic mechanisms underlying many of the HAB illnesses have been well delineated, the literature examining the neuropsychological impairments is unclear and needs to be defined. This review is intended to introduce an emerging area of study linking HAB illnesses with neuropsychological changes. JF - Journal of the International Neuropsychological Society : JINS AU - Friedman, Melissa A AU - Levin, Bonnie E AD - The NIEHS Marine and Freshwater Biomedical Sciences Center, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Florida, USA. melissafried@yahoo.com Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 331 EP - 338 VL - 11 IS - 3 SN - 1355-6177, 1355-6177 KW - Index Medicus KW - National Library of Medicine KW - Amnesia: diagnosis KW - *Amnesia: etiology KW - *Ciguatera Poisoning: complications KW - *Ciguatera Poisoning: etiology KW - Humans KW - Neuropsychological Tests KW - *Pfiesteria piscicida UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85378910?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+International+Neuropsychological+Society+%3A+JINS&rft.atitle=Neurobehavioral+effects+of+harmful+algal+bloom+%28HAB%29+toxins%3A+a+critical+review.&rft.au=Friedman%2C+Melissa+A%3BLevin%2C+Bonnie+E&rft.aulast=Friedman&rft.aufirst=Melissa&rft.date=2005-05-01&rft.volume=11&rft.issue=3&rft.spage=331&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+International+Neuropsychological+Society+%3A+JINS&rft.issn=13556177&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Effect of catechol-O-methyltransferase val158met genotype on attentional control. AN - 85307071; pmid-15901785 AB - The cingulate cortex is richly innervated by dopaminergic projections and plays a critical role in attentional control (AC). Evidence indicates that dopamine enhances the neurophysiological signal-to-noise ratio and that dopaminergic tone in the frontal cortex is critically dependent on catechol-O-methyltransferase (COMT). A functional polymorphism (val158met) in the COMT gene accounts for some of the individual variability in executive function mediated by the dorsolateral prefrontal cortex. We explored the effect of this genetic polymorphism on cingulate engagement during a novel AC task. We found that the COMT val158met polymorphism also affects the function of the cingulate during AC. Individuals homozygous for the high-activity valine ("val") allele show greater activity and poorer performance than val/methionine ("met") heterozygotes, who in turn show greater activity and poorer performance than individuals homozygous for the low-activity met allele, and these effects are most evident at the highest demand for AC. These results indicate that met allele load and presumably enhanced dopaminergic tone improve the "efficiency" of local circuit processing within the cingulate cortex and thereby its function during AC. JF - The Journal of Neuroscience AU - Blasi Giuseppe AU - Mattay, Venkata S AU - Bertolino Alessandro AU - Elvevåg Brita AU - Callicott, Joseph H AU - Das Saumitra AU - Kolachana Bhaskar S AU - Egan, Michael F AU - Goldberg, Terry E AU - Weinberger, Daniel R AD - Clinical Brain Disorders Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1379, USA. PY - 2005 SP - 5038 EP - 5045 VL - 25 IS - 20 SN - 0270-6474, 0270-6474 KW - Magnetic Resonance Imaging KW - Regression Analysis KW - Analysis of Variance KW - Humans KW - Prefrontal Cortex KW - Methionine KW - Genotype KW - Oxygen KW - Photic Stimulation KW - Brain Mapping KW - Comparative Study KW - Catechol O-Methyltransferase KW - Adult KW - Case-Control Studies KW - Neuropsychological Tests KW - Image Processing, Computer-Assisted KW - Attention KW - Male KW - Female KW - Reaction Time KW - Valine KW - Polymorphism, Genetic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85307071?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Neuroscience&rft.atitle=Effect+of+catechol-O-methyltransferase+val158met+genotype+on+attentional+control.&rft.au=Blasi+Giuseppe%3BMattay%2C+Venkata+S%3BBertolino+Alessandro%3BElvev%C3%A5g+Brita%3BCallicott%2C+Joseph+H%3BDas+Saumitra%3BKolachana+Bhaskar+S%3BEgan%2C+Michael+F%3BGoldberg%2C+Terry+E%3BWeinberger%2C+Daniel+R&rft.aulast=Blasi+Giuseppe&rft.aufirst=&rft.date=2005-05-01&rft.volume=25&rft.issue=20&rft.spage=5038&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - An HIV vaccine: as we build it, will they come? AN - 838989665; 3349250 JF - Health affairs AU - McCluskey, Margaret M AU - Alexander, Sarah B AU - Larkin, Brenda D AU - Murguia, Matthew AU - Wakefield, Steven AD - National Institutes of Health ; Fred Hutchinson Cancer Research Center ; NIH Office of Program Operations and Scientific Information Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 643 EP - 652 VL - 24 IS - 3 SN - 0278-2715, 0278-2715 KW - Sociology KW - World Health Organization KW - Prevention KW - Health care KW - Medical research KW - AIDS KW - Policy making KW - Medical treatment KW - HIV KW - Private sector KW - Public health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/838989665?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+affairs&rft.atitle=An+HIV+vaccine%3A+as+we+build+it%2C+will+they+come%3F&rft.au=McCluskey%2C+Margaret+M%3BAlexander%2C+Sarah+B%3BLarkin%2C+Brenda+D%3BMurguia%2C+Matthew%3BWakefield%2C+Steven&rft.aulast=McCluskey&rft.aufirst=Margaret&rft.date=2005-05-01&rft.volume=24&rft.issue=3&rft.spage=643&rft.isbn=&rft.btitle=&rft.title=Health+affairs&rft.issn=02782715&rft_id=info:doi/10.1377%2Fhlthaff.24.3.643 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 7890 5792 10484; 5703 3617 6220; 7886 10902; 482 3617 6220; 10449 5772; 9625 9628; 10208; 5775 13521; 10072; 13737 6772 9030 DO - http://dx.doi.org/10.1377/hlthaff.24.3.643 ER - TY - JOUR T1 - Two C-Methyl Derivatives of [ super(11)C]WAY-100635 - Effects of an Amido a-Methyl Group on Metabolism and Brain 5-HT sub(1A) Receptor Radioligand Behavior in Monkey* AN - 754566982; 13409466 AB - [carbonyl- super(11)C]N-(2-(1-(4-(2-methoxyphenyl)-piperazinyl)ethyl)- N-pyridinyl)cyclohexanecarboxamide ([carbonyl- super(11)C]WAY-1006 35) is an effective radioligand for imaging brain 5-HT sub(1A) receptors with positron emission tomography (PET). However, this radioligand has some drawbacks for deriving relative regional receptor densities, including rapid metabolism, which acts against accurate definition of an arterial input function for compartmental modeling, and very low nonspecific binding in brain, which detracts from the accuracy of modeling by a simplified reference tissue (cerebellum) approach. Here, in a search for a radioligand that overcomes these limitations, we investigated the effects of introducing a single methyl group at either of the carbon atoms alpha to the amide bond in [ super(11)C]WAY-100635. Ligands with a methyl group on the alpha carbon of the cyclohexyl group (SWAY) or the alpha carbon of the C sub(2)H sub(4) linker ((R,S)-JWAY) were synthesized and tested for binding affinity and intrinsic activity at 5-HT sub(1A) receptors. SWAY was labeled with carbon-11 (t sub(1/2) = 20.4 minutes; b super(+) = 99.8%) in its O-methyl group and (R,S)-JWAY in its carbonyl group. Each radioligand was evaluated by PET experiments in cynomolgus monkey. SWAY and (R,S)-JWAY were found to be high-affinity antagonists at 5-HT sub(1A) receptors. After injection of [ super(11)C]SWAY into monkey, radioactivity uptake in brain reached a maximum of 3% at 4.5 minutes and decreased to 0.7% at 72 minutes. However, over the time span of the experiment, radioactivity concentrations in 5-HT sub(1A) receptor-rich brain regions were only fractionally higher than in cerebellum. Radioactivity represented by parent radioligand in plasma was 39% at 45 minutes. After injection of [ super(11)C](R,S)-JWAY alone, radioactivity uptake in brain reached a maximum of 4.8% at 2.5 minutes and decreased to 1.2% at 90 minutes. At this time, radioactivity concentration in 5-HT sub(1A) receptor-rich brain regions was markedly greater than in cerebellum. In another PET experiment, the monkey was predosed with WAY-100635 before [ super(11)C](R,S)-JWAY injection. At 90 minutes after injection, the ratio of radioactivity in 5-HT sub(1A) receptor-rich regions to that in cerebellum was reduced to near unity. Radioactivity represented by parent radioligand in plasma was 12% at 45 minutes. [ super(11)C](R,S)- JWAY, but not [ super(11)C]SWAY, gives a sizeable 5-HT sub(1A) receptor-selective PET signal in monkey. The presence of a C-methyl group adjacent to the amide bond in SWAY or (R,S)-JWAY fails to counter metabolism. JF - Molecular Imaging and Biology AU - McCarron, Julie A AU - Marchais-Oberwinkler, Sandrine AU - Pike, Victor W AU - Tarkiainen, Jari AU - Halldin, Christer AU - Sovago, Judit AU - Gulyas, Balazs AU - Wikstroem, Hakan V AU - Farde, Lars AD - MRC Cyclotron Unit, Imperial College School of Medicine, Hammersmith Hospital, Ducane Road, London, W12 0NN, UK, mccarronj@intra.nimh.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 209 EP - 219 PB - Springer-Verlag, Tiergartenstrasse 17 Heidelberg 69121 Germany VL - 7 IS - 3 SN - 1536-1632, 1536-1632 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Cerebellum KW - Brain KW - Carbon cycle KW - Antagonists KW - Carbon KW - Serotonin S1 receptors KW - Receptor density KW - Positron emission tomography KW - Radioisotopes KW - Cynomolgus KW - Radioactivity KW - carbonyls KW - amides KW - Metabolism KW - W 30910:Imaging KW - N3 11150:General and miscellaneous topics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/754566982?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Imaging+and+Biology&rft.atitle=Two+C-Methyl+Derivatives+of+%5B+super%2811%29C%5DWAY-100635+-+Effects+of+an+Amido+a-Methyl+Group+on+Metabolism+and+Brain+5-HT+sub%281A%29+Receptor+Radioligand+Behavior+in+Monkey*&rft.au=McCarron%2C+Julie+A%3BMarchais-Oberwinkler%2C+Sandrine%3BPike%2C+Victor+W%3BTarkiainen%2C+Jari%3BHalldin%2C+Christer%3BSovago%2C+Judit%3BGulyas%2C+Balazs%3BWikstroem%2C+Hakan+V%3BFarde%2C+Lars&rft.aulast=McCarron&rft.aufirst=Julie&rft.date=2005-05-01&rft.volume=7&rft.issue=3&rft.spage=209&rft.isbn=&rft.btitle=&rft.title=Molecular+Imaging+and+Biology&rft.issn=15361632&rft_id=info:doi/10.1007%2Fs11307-005-4127-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-08-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Neuroimaging; Carbon cycle; Brain; Cerebellum; Antagonists; Carbon; Serotonin S1 receptors; Radioisotopes; Positron emission tomography; Receptor density; Radioactivity; amides; carbonyls; Metabolism; Cynomolgus DO - http://dx.doi.org/10.1007/s11307-005-4127-5 ER - TY - JOUR T1 - Cyclooxygenase-2 expression is associated with increased size in human sporadic colorectal adenomas. AN - 68572842; 16158946 AB - Cyclooxygenase-2 (COX-2) has been implicated in colorectal carcinogenesis but its role is not completely defined. The expression of COX-2 was evaluated in 68 paraffin-embedded sporadic colorectal adenomas by immunohistochemistry. Associations between COX-2 expression and the clinicopathological characteristics of the adenomas were studied by contingency tables and the Chi-square test. Cytoplasmic staining for COX-2 protein was present in epithelial cells of 62 out of the 68 adenomas. COX-2 expression was not associated with age or gender. Furthermore, no significant correlations were found between the expression of the protein and histology (tubular vs tubulovillous), localization (proximal vs distal) or morphology (sessil vs pedunculated) of the adenomas. Both stromal and epithelial COX-2 expressions were higher in larger (>4 mm) compared with smaller (< or =4 mm) adenomas (p =0. 037 and p=0. 024). These data support the hypothesis that the expression of COX-2 may occur as a general phenomenon in colorectal adenomas. A size-dependent increase of COX-2 expression might be involved in colorectal carcinogenesis. JF - Anticancer research AU - Pisano, Carmela AU - Ottaiano, Alessandro AU - Tatangelo, Fabiana AU - Di Bonito, Maurizio AU - Falanga, Marzia AU - Iaffaioli, Vincenzo Rosario AU - Botti, Gerardo AU - Pignata, Sandro AU - Acquaviva, Angela Maria AD - Medical Oncology B, National Cancer Institute, via Mariano Semmola, 80131, Naples, Italy. carmen_pisano@libero.it PY - 2005 SP - 2065 EP - 2068 VL - 25 IS - 3B SN - 0250-7005, 0250-7005 KW - Membrane Proteins KW - 0 KW - Cyclooxygenase 2 KW - EC 1.14.99.1 KW - PTGS2 protein, human KW - Prostaglandin-Endoperoxide Synthases KW - Index Medicus KW - Paraffin Embedding KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Male KW - Female KW - Adenoma -- enzymology KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- enzymology KW - Adenoma -- pathology KW - Prostaglandin-Endoperoxide Synthases -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68572842?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+research&rft.atitle=Cyclooxygenase-2+expression+is+associated+with+increased+size+in+human+sporadic+colorectal+adenomas.&rft.au=Pisano%2C+Carmela%3BOttaiano%2C+Alessandro%3BTatangelo%2C+Fabiana%3BDi+Bonito%2C+Maurizio%3BFalanga%2C+Marzia%3BIaffaioli%2C+Vincenzo+Rosario%3BBotti%2C+Gerardo%3BPignata%2C+Sandro%3BAcquaviva%2C+Angela+Maria&rft.aulast=Pisano&rft.aufirst=Carmela&rft.date=2005-05-01&rft.volume=25&rft.issue=3B&rft.spage=2065&rft.isbn=&rft.btitle=&rft.title=Anticancer+research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-02 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Alcohol use disorder and illicit drug use in admissions to general hospitals in the United States. AN - 68033442; 16019976 AB - This study estimated the prevalence and explored the management of illicit drug use, illicit drug use associated with alcohol use disorder (AUD), and AUD without reported illicit drug use in a national sample of 2040 admissions to general hospitals in the United States. Surveyed in 1994, admissions were diagnosed with past 12-month DSM-IV AUD according to the Alcohol Use Disorders and Associated Disabilities Interview Schedule. Information about drug use was also included in the interview. Entries in hospital records were used to operationalize management. Prevalence of chronic drug use in hospital admissions was 5%, 14% in 18-44-year-old admissions, and 31% in admissions with an AUD. In admissions with an AUD, 45% reported no drug use. Detection rates were 82% for admissions with comorbid AUD and chronic drug use (where detection of either problem was assessed); detection rates hovered around 50% in admissions with one or the other condition. Low rates of treatment and referral (33% and 42%, respectively) were observed in the comorbid group; rates were 13-17% in admissions with AUD alone or illicit drug use alone. Findings indicate the need for increased attention to drug use and to AUD with and without other drug use among general hospital admissions. JF - The American journal on addictions AU - Smothers, Barbara A AU - Yahr, Harold T AD - Division of Epidemiology and Prevention Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA. bs86h@nih.gov PY - 2005 SP - 256 EP - 267 VL - 14 IS - 3 SN - 1055-0496, 1055-0496 KW - Street Drugs KW - 0 KW - Index Medicus KW - Mass Screening KW - Medical Records KW - Length of Stay KW - Humans KW - Adult KW - Interviews as Topic KW - Hospitals, General KW - Adolescent KW - United States -- epidemiology KW - Male KW - Female KW - Prevalence KW - Alcohol-Related Disorders -- diagnosis KW - Substance-Related Disorders -- diagnosis KW - Patient Admission KW - Substance-Related Disorders -- epidemiology KW - Alcohol-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68033442?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+on+addictions&rft.atitle=Alcohol+use+disorder+and+illicit+drug+use+in+admissions+to+general+hospitals+in+the+United+States.&rft.au=Smothers%2C+Barbara+A%3BYahr%2C+Harold+T&rft.aulast=Smothers&rft.aufirst=Barbara&rft.date=2005-05-01&rft.volume=14&rft.issue=3&rft.spage=256&rft.isbn=&rft.btitle=&rft.title=The+American+journal+on+addictions&rft.issn=10550496&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-30 N1 - Date created - 2005-07-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted disruption of p53 attenuates doxorubicin-induced cardiac toxicity in mice. AN - 68029615; 16013437 AB - Use of the chemotherapeutic agent doxorubicin (Dox) is limited by dose-dependent cardiotoxic effects. The molecular mechanism underlying these toxicities are incompletely understood, but previous results have demonstrated that Dox induces p53 expression. Because p53 is an important regulator of the cell birth and death we hypothesized that targeted disruption of the p53 gene would attenuate Dox-induced cardiotoxicity. To test this, female 6-8 wk old C57BL wild-type (WT) or p53 knockout (p53 KO) mice were randomized to either saline or Dox 20 mg/kg via intraperitoneal injection. Animals were serially imaged with high-frequency (14 MHz) two-dimensional echocardiography. Measurements of left ventricle (LV) systolic function as assessed by fractional shortening (FS) demonstrated a decline in WT mice as early as 4 days after Dox injection and by 2 wk demonstrated a reduction of 31 +/- 16% (P < 0.05) from the baseline. In contrast, in p53 KO mice, LV FS was unchanged over the 2 wk period following Dox injection. Apoptosis of cardiac myocytes as measured by the TUNEL and ligase reactions were significantly increased at 24 h after Dox treatment in WT mice but not in p53 KO mice. After Dox injection, levels of myocardial glutathione and Cu/Zn superoxide dismutase were preserved in p53 KO mice, but not in WT animals. These observations suggest that p53 mediated signals are likely to play a significant role in Dox-induced cardiac toxicity and that they may modulate Dox-induced oxidative stress. JF - Molecular and cellular biochemistry AU - Shizukuda, Yukitaka AU - Matoba, Satoaki AU - Mian, Omar Y AU - Nguyen, Tammy AU - Hwang, Paul M AD - Cardiovascular Branch, National Heart Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. shizukuy@nhlbi.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 25 EP - 32 VL - 273 IS - 1-2 SN - 0300-8177, 0300-8177 KW - Antibiotics, Antineoplastic KW - 0 KW - Cdkn1a protein, mouse KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - Reactive Oxygen Species KW - Tumor Suppressor Protein p53 KW - Doxorubicin KW - 80168379AG KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Apoptosis KW - Myocardium -- pathology KW - Glutathione -- metabolism KW - Superoxide Dismutase -- metabolism KW - Mice KW - Myocytes, Cardiac -- pathology KW - Myocardium -- metabolism KW - Cell Cycle Proteins -- metabolism KW - Mice, Knockout KW - In Situ Nick-End Labeling KW - Oxidative Stress KW - Mice, Inbred C57BL KW - Female KW - Male KW - Tumor Suppressor Protein p53 -- physiology KW - Cardiomyopathies -- metabolism KW - Cardiomyopathies -- prevention & control KW - Heart -- drug effects KW - Doxorubicin -- toxicity KW - Heart -- physiopathology KW - Antibiotics, Antineoplastic -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68029615?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biochemistry&rft.atitle=Targeted+disruption+of+p53+attenuates+doxorubicin-induced+cardiac+toxicity+in+mice.&rft.au=Shizukuda%2C+Yukitaka%3BMatoba%2C+Satoaki%3BMian%2C+Omar+Y%3BNguyen%2C+Tammy%3BHwang%2C+Paul+M&rft.aulast=Shizukuda&rft.aufirst=Yukitaka&rft.date=2005-05-01&rft.volume=273&rft.issue=1-2&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biochemistry&rft.issn=03008177&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-07-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Animal models for acquired bone marrow failure syndromes. AN - 68028036; 16012128 AB - Bone marrow failure is a disease characterized by a drastic decline in the marrow's functional ability to produce mature blood cells. Aplastic anemia, a disease in which patients have essentially empty bone marrow accompanied by severe anemia, neutropenia, and thrombocytopenia, presents a paradigm for bone marrow failure. Damage to the marrow may first result from exposure to toxic chemicals, drug overdose, radiation, and viral infection; however, it is the extended immune-mediated reaction that causes massive destruction of hematopoietic cells and leads to marrow hypoplasia and peripheral pancytopenia. In recent years, animal models of acquired bone marrow failure syndromes have helped to strengthen our understanding of the mechanisms causing bone marrow failure. In this review, animal models for bone marrow failure are summarized by two groups: 1) bone marrow failure induced by toxic chemicals and drugs such as benzene, busulfan, and chloramphenicol, and radiation, and 2) models developed by an immune-related mechanism such as viral infection or foreign lymphocyte infusion. JF - Clinical medicine & research AU - Chen, Jichun AD - Hematology Branch, NHLBI, NIH Building 10, Clinical Research Center, Room 3E-5132, Bethesda, MD 20892-1202, USA. chenji@nhlbi.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 102 EP - 108 VL - 3 IS - 2 SN - 1539-4182, 1539-4182 KW - Chloramphenicol KW - 66974FR9Q1 KW - Busulfan KW - G1LN9045DK KW - Benzene KW - J64922108F KW - Index Medicus KW - Animals KW - Anemia, Aplastic -- etiology KW - Virus Diseases -- complications KW - Syndrome KW - Humans KW - Chloramphenicol -- toxicity KW - Benzene -- toxicity KW - Disease Models, Animal KW - Radiation Injuries, Experimental -- etiology KW - Mice KW - Busulfan -- toxicity KW - Bone Marrow Diseases -- etiology KW - Bone Marrow Diseases -- chemically induced KW - Bone Marrow Diseases -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68028036?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+medicine+%26+research&rft.atitle=Animal+models+for+acquired+bone+marrow+failure+syndromes.&rft.au=Chen%2C+Jichun&rft.aulast=Chen&rft.aufirst=Jichun&rft.date=2005-05-01&rft.volume=3&rft.issue=2&rft.spage=102&rft.isbn=&rft.btitle=&rft.title=Clinical+medicine+%26+research&rft.issn=15394182&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-02 N1 - Date created - 2005-07-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Exp Pathol. 2002 Oct;83(5):225-38 [12641819] N Engl J Med. 2005 Apr 7;352(14):1413-24 [15814878] Cancer Res. 2003 Sep 1;63(17):5414-9 [14500376] Rev Med Virol. 2003 Nov-Dec;13(6):347-59 [14625883] N Engl J Med. 2004 Feb 5;350(6):586-97 [14762186] Lancet. 2004 Jul 24-30;364(9431):355-64 [15276395] Blood. 2004 Sep 15;104(6):1671-8 [15166031] Stem Cells. 2004;22(5):750-8 [15342939] Br J Haematol. 1967 Jul;13(4):482-91 [6029951] Blood. 1974 Jul;44(1):49-56 [4834516] Br J Cancer. 1975 Aug;32(2):193-8 [764844] Br J Haematol. 1976 Apr;32(4):525-31 [1259934] Blood. 1978 Apr;51(4):601-10 [305267] Exp Hematol. 1978 Nov;6(10):791-800 [33819] Int J Radiat Oncol Biol Phys. 1979 Sep;5(9):1621-5 [395140] J Lab Clin Med. 1980 Jul;96(1):36-46 [7391656] Cell Tissue Kinet. 1981 Jan;14(1):8590 [7471158] Exp Hematol. 1980 Jul;8(6):788-94 [7202582] Exp Hematol. 1982 Jan;10(1):20-5 [7060658] J Cell Physiol. 1982 Sep;112(3):345-52 [7130283] Exp Hematol. 1983 Jul;11(6):542-52 [6617789] Blut. 1984 May;48(5):277-84 [6722358] Int J Exp Pathol. 2003 Feb;84(1):31-48 [12694485] Food Chem Toxicol. 2000 Oct;38(10):925-38 [11039326] Food Chem Toxicol. 2001 Apr;39(4):375-83 [11295484] Toxicology. 2001 May 21;162(3):179-91 [11369114] Toxicol Appl Pharmacol. 2001 Jul 15;174(2):139-45 [11446829] Life Sci. 2001 Aug 10;69(12):1373-9 [11531161] J Clin Invest. 2001 Sep;108(5):765-73 [11544283] Ann Intern Med. 2002 Apr 2;136(7):534-46 [11926789] Food Chem Toxicol. 2002 Dec;40(12):1849-61 [12419700] Int J Cell Cloning. 1984 Jul;2(4):263-71 [6379064] Blood. 1984 Nov;64(5):1036-41 [6487805] Anticancer Res. 1985 Jan-Feb;5(1):101-10 [3888044] Int J Cell Cloning. 1986 Sep;4(5):357-67 [3772175] Exp Hematol. 1987 Mar;15(3):269-75 [3493173] J Exp Pathol. 1987;3(3):259-69 [2835466] Exp Hematol. 1989 May;17(4):335-9 [2468513] Toxicol Lett. 1991 Apr;56(1-2):159-66 [2017773] Blood. 1991 Aug 15;78(4):938-44 [1868253] Viral Immunol. 1991 Winter;4(4):269-80 [1668061] Exp Hematol. 1994 Jul;22(7):573-81 [8013573] Arch Toxicol. 1995;69(3):141-8 [7717869] Blood. 1995 Jun 1;85(11):3183-90 [7538820] J Toxicol Environ Health. 1995 Oct;46(2):183-201 [7563217] Br J Haematol. 1995 Sep;91(1):245-52 [7577642] Blood. 1996 May 15;87(10):4149-57 [8639773] Leuk Lymphoma. 1996 Apr;21(3-4):217-23 [8726402] J Virol. 1997 Jun;71(6):4589-98 [9151853] Hum Exp Toxicol. 1998 Jan;17(1):8-17 [9491332] J Exp Med. 1998 Jun 1;187(11):1903-20 [9607930] Exp Hematol. 1999 May;27(5):895-903 [10340406] Hum Exp Toxicol. 1999 Sep;18(9):566-76 [10523871] Exp Hematol. 2004 Dec;32(12):1163-72 [15588941] Leukemia. 2005 Feb;19(2):217-22 [15668701] Comment In: Clin Med Res. 2005 May;3(2):63-4 [16012122] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibitors of histone deacetylases alter kinetochore assembly by disrupting pericentromeric heterochromatin. AN - 67956052; 15846093 AB - The kinetochore, a multi-protein complex assembled on centromeric chromatin in mitosis, is essential for sister chromosome segregation. We show here that inhibition of histone deacetylation blocks mitotic progression at prometaphase in two human tumor cell lines by interfering with kinetochore assembly. Decreased amounts of hBUB1, CENP-F and the motor protein CENP-E were present on kinetochores of treated cells. These kinetochores failed to nucleate and inefficiently captured microtubules, resulting in activation of the mitotic checkpoint. Addition of histone deacetylase inhibitors prior to the end of S-phase resulted in decreased HP1-beta on pericentromeric heterochromatin in S-phase and G(2), decreased pericentromeric targeting of Aurora B kinase, resulting in decreased premitotic phosphorylation of pericentromeric histone H3(S10) in G(2), followed by assembly of deficient kinetochores in M-phase. HP1-beta, Aurora B and the affected kinetochore proteins all were present at normal levels in treated cells; thus, effects of the inhibitors on mitotic progression do not seem to reflect changes in gene expression. In vitro kinase activity of Aurora B isolated from treated cells was unaffected. We propose that the increased presence in pericentromeric heterochromatin of histone H3 acetylated at K9 is responsible for the mitotic defects resulting from inhibition of histone deacetylation. JF - Cell cycle (Georgetown, Tex.) AU - Robbins, April R AU - Jablonski, Sandra A AU - Yen, Tim J AU - Yoda, Kinya AU - Robey, Rob AU - Bates, Susan E AU - Sackett, Dan L AD - Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA. cwharr@helix.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 717 EP - 726 VL - 4 IS - 5 KW - Chromosomal Proteins, Non-Histone KW - 0 KW - Depsipeptides KW - Heterochromatin KW - Histone Deacetylase Inhibitors KW - Histones KW - Hydroxamic Acids KW - Indoles KW - Microcystins KW - Microfilament Proteins KW - Peptides, Cyclic KW - centromere protein E KW - centromere protein F KW - heterochromatin-specific nonhistone chromosomal protein HP-1 KW - 107283-02-3 KW - trichostatin A KW - 3X2S926L3Z KW - DAPI KW - 47165-04-8 KW - HC toxin KW - 83209-65-8 KW - romidepsin KW - CX3T89XQBK KW - Protein Kinases KW - EC 2.7.- KW - AURKB protein, human KW - EC 2.7.11.1 KW - Aurora Kinase B KW - Aurora Kinases KW - BUB1 protein, human KW - Bub1 spindle checkpoint protein KW - Protein-Serine-Threonine Kinases KW - Histone Deacetylases KW - EC 3.5.1.98 KW - cyanoginosin LR KW - EQ8332842Y KW - Methotrexate KW - YL5FZ2Y5U1 KW - Index Medicus KW - Protein Processing, Post-Translational -- drug effects KW - Protein Kinases -- analysis KW - Humans KW - Cell Division -- physiology KW - Chromosomal Proteins, Non-Histone -- metabolism KW - G2 Phase -- physiology KW - Chromosomal Proteins, Non-Histone -- analysis KW - Protein-Serine-Threonine Kinases -- analysis KW - Histone Deacetylases -- metabolism KW - Spindle Apparatus -- physiology KW - Peptides, Cyclic -- pharmacology KW - Hydroxamic Acids -- pharmacology KW - Protein-Serine-Threonine Kinases -- physiology KW - S Phase -- drug effects KW - Methotrexate -- pharmacology KW - Protein-Serine-Threonine Kinases -- drug effects KW - S Phase -- physiology KW - Cell Division -- drug effects KW - Cell Line, Tumor KW - Spindle Apparatus -- drug effects KW - Acetylation KW - G2 Phase -- drug effects KW - Depsipeptides -- pharmacology KW - Indoles -- pharmacology KW - Centromere -- physiology KW - Kinetochores -- drug effects KW - Heterochromatin -- drug effects KW - Kinetochores -- physiology KW - Heterochromatin -- physiology KW - Kinetochores -- chemistry KW - Chromosome Segregation -- drug effects KW - Heterochromatin -- chemistry KW - Histones -- metabolism KW - Mitosis -- drug effects KW - Centromere -- drug effects KW - Mitosis -- physiology KW - Histones -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67956052?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.atitle=Inhibitors+of+histone+deacetylases+alter+kinetochore+assembly+by+disrupting+pericentromeric+heterochromatin.&rft.au=Robbins%2C+April+R%3BJablonski%2C+Sandra+A%3BYen%2C+Tim+J%3BYoda%2C+Kinya%3BRobey%2C+Rob%3BBates%2C+Susan+E%3BSackett%2C+Dan+L&rft.aulast=Robbins&rft.aufirst=April&rft.date=2005-05-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-03 N1 - Date created - 2005-06-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prenatal marijuana exposure: effect on child depressive symptoms at ten years of age. AN - 67911045; 15869861 AB - Studies of the consequences of prenatal marijuana use have reported effects predominantly on the behavioral and cognitive development of the children. Research on other aspects of child neurobehavioral development, such as psychiatric symptomatology, has been limited. This study examines the relations between prenatal marijuana exposure (PME) and child depressive symptoms at 10 years of age. Data are from the 10-year follow-up of 633 mother-child dyads who participated in the Maternal Health Practices and Child Development Project. Maternal prenatal and current substance use, measures of the home environment, demographic status, and psychosocial characteristics were ascertained at prenatal months four and seven, at delivery, and at age 10. At age 10, the children also completed the Children's Depression Inventory (CDI) [M. Kovacs. The Children's Depression Inventory, Multi-Health Systems, Inc., North Tonawanda, NY, (1992).], a self-report measure of current depressive symptoms. Multivariate regressions were used to test trimester-specific effects of marijuana and their associations with the CDI total score, while controlling for significant prenatal predictors and significant current covariates of childhood depression. PME in the first and third trimesters predicted significantly increased levels of depressive symptoms. This finding remained significant after controlling for all identified covariates from both the prenatal period and the current phase at age 10. These findings reflect an association with the level of depressive symptoms rather than a diagnosis of a major depressive disorder. Other significant correlates of depressive symptoms in the children included maternal education, maternal tobacco use (prenatal or current), and the child's composite IQ score. These findings are consistent with recent reports that identify specific areas of the brain and specific brain functions that are associated with PME. JF - Neurotoxicology and teratology AU - Gray, Kimberly A AU - Day, Nancy L AU - Leech, Sharon AU - Richardson, Gale A AD - Susceptibility and Population Health Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. PY - 2005 SP - 439 EP - 448 VL - 27 IS - 3 SN - 0892-0362, 0892-0362 KW - Index Medicus KW - Environment KW - Humans KW - Social Behavior KW - Mothers -- psychology KW - Child KW - Pregnancy KW - Socioeconomic Factors KW - Psychiatric Status Rating Scales KW - Pregnancy Trimester, First KW - Prospective Studies KW - Adult KW - Cohort Studies KW - Female KW - Male KW - Substance-Related Disorders -- epidemiology KW - Intelligence Tests KW - Depressive Disorder -- epidemiology KW - Depressive Disorder -- chemically induced KW - Cannabis -- adverse effects KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67911045?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=proceeding&rft.jtitle=Neurotoxicology+and+teratology&rft.atitle=Prenatal+marijuana+exposure%3A+effect+on+child+depressive+symptoms+at+ten+years+of+age.&rft.au=Gray%2C+Kimberly+A%3BDay%2C+Nancy+L%3BLeech%2C+Sharon%3BRichardson%2C+Gale+A&rft.aulast=Gray&rft.aufirst=Kimberly&rft.date=2005-05-01&rft.volume=27&rft.issue=3&rft.spage=439&rft.isbn=&rft.btitle=&rft.title=Neurotoxicology+and+teratology&rft.issn=08920362&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-02 N1 - Date created - 2005-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Development of medications for alcohol use disorders: recent advances and ongoing challenges. AN - 67900958; 15934870 AB - During the past decade, efforts to develop medications for alcoholism have burgeoned. Three agents, disulfiram, naltrexone and acamprosate, are now approved in a large number of countries. Although many patients have benefited from existing medications, their effects are moderate, and some alcoholics fail to respond to them. A host of new agents are currently under active investigation. Critical barriers must be overcome to ensure that future efforts in the development of medications for alcohol use disorders reach full fruition. These challenges include: establishing key targets for drug discovery; validating animal and human screening models; and developing biomarkers to help predict treatment success. In addition, it is important to formulate methodological and statistical strategies to efficiently conduct alcohol pharmacotherapy trials; to specify genetic and phenotypic patient characteristics associated with efficacy and safety for lead compounds; to forge productive alliances among governmental agencies, the pharmaceutical industry and academic researchers to further drug development; and, ultimately and perhaps most difficult, to engage the practitioner community to incorporate medications into the alcohol treatment process. JF - Expert opinion on emerging drugs AU - Litten, Raye Z AU - Fertig, Joanne AU - Mattson, Margaret AU - Egli, Mark AD - Division of Treatment and Recovery Research, National Institute on Alcohol Abuse and Alcoholism, 5635 Fishers Lane, Room 2041, Bethesda, MD 20852-1705, USA. rlitten@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 323 EP - 343 VL - 10 IS - 2 KW - Drugs, Investigational KW - 0 KW - Index Medicus KW - Humans KW - Clinical Trials as Topic -- statistics & numerical data KW - Drugs, Investigational -- therapeutic use KW - Alcohol-Related Disorders -- physiopathology KW - Alcohol-Related Disorders -- drug therapy KW - Drug Industry -- trends KW - Drugs, Investigational -- chemistry KW - Alcohol-Related Disorders -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67900958?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Expert+opinion+on+emerging+drugs&rft.atitle=Development+of+medications+for+alcohol+use+disorders%3A+recent+advances+and+ongoing+challenges.&rft.au=Litten%2C+Raye+Z%3BFertig%2C+Joanne%3BMattson%2C+Margaret%3BEgli%2C+Mark&rft.aulast=Litten&rft.aufirst=Raye&rft.date=2005-05-01&rft.volume=10&rft.issue=2&rft.spage=323&rft.isbn=&rft.btitle=&rft.title=Expert+opinion+on+emerging+drugs&rft.issn=1744-7623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-04-14 N1 - Date created - 2005-06-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Disease and injury among participants in the Agricultural Health Study. AN - 67891291; 15931940 AB - The Agricultural Health Study (www.aghealth.org) is a cohort of 89,658 pesticide applicators and their spouses from Iowa and North Carolina assembled between 1993 and 1997 to evaluate riskfactorsfor disease in ruralfarm populations. This prospective study is just now reaching sufficient maturity for analysis of many disease endpoints. Nonetheless, several analyses have already provided interesting and important leads regarding disease patterns in agricultural populations and etiologic clues for the general population. Compared to the mortality experience of the general population in the two states (adjusted for race, gender, age and calendar time), the cohort experienced a very low mortality rate overall and for many specific causes and a low rate of overall cancer incidence. A few cancers, however, appear elevated, including multiple myeloma and cancers of the lip, gallbladder, ovary, prostate, and thyroid, but numbers are small for many cancers. A study of prostate cancer found associations with exposure to several pesticides, particularly among individuals with a family history of prostate cancer. Links to pesticides and other agricultural factors have been found for injuries, retinal degeneration, and respiratory wheeze. Methodological studies have determined that information collected by interview is unbiased and reliable. A third round of interviews scheduled to begin in 2005 will collect additional information on agricultural exposures and health outcomes. The study can provide data to address many health issues in the agricultural community. The study investigators welcome collaboration with interested scientists. JF - Journal of agricultural safety and health AU - Blair, A AU - Sandler, D AU - Thomas, K AU - Hoppin, J A AU - Kamel, F AU - Coble, J AU - Lee, W J AU - Rusiecki, J AU - Knott, C AU - Dosemeci, M AU - Lynch, C F AU - Lubin, J AU - Alavanja, M AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Executive Plaza South, Room 8118, Bethesda, Maryland 20892 , USA. blaira@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 141 EP - 150 VL - 11 IS - 2 SN - 1074-7583, 1074-7583 KW - Pesticides KW - 0 KW - Index Medicus KW - Prospective Studies KW - Risk Factors KW - Humans KW - Accidents, Occupational -- statistics & numerical data KW - Wounds and Injuries KW - Interviews as Topic KW - Middle Aged KW - North Carolina -- epidemiology KW - Male KW - Iowa -- epidemiology KW - Female KW - Spouses KW - Agricultural Workers' Diseases -- mortality KW - Agricultural Workers' Diseases -- etiology KW - Agricultural Workers' Diseases -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67891291?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+agricultural+safety+and+health&rft.atitle=Disease+and+injury+among+participants+in+the+Agricultural+Health+Study.&rft.au=Blair%2C+A%3BSandler%2C+D%3BThomas%2C+K%3BHoppin%2C+J+A%3BKamel%2C+F%3BCoble%2C+J%3BLee%2C+W+J%3BRusiecki%2C+J%3BKnott%2C+C%3BDosemeci%2C+M%3BLynch%2C+C+F%3BLubin%2C+J%3BAlavanja%2C+M&rft.aulast=Blair&rft.aufirst=A&rft.date=2005-05-01&rft.volume=11&rft.issue=2&rft.spage=141&rft.isbn=&rft.btitle=&rft.title=Journal+of+agricultural+safety+and+health&rft.issn=10747583&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-06-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Am J Epidemiol. 2003 May 1;157(9):800-14 [12727674] Occup Environ Med. 2003 Aug;60(8):e3 [12883030] Scand J Work Environ Health. 2005;31 Suppl 1:39-45; discussion 5-7 [16190148] Environ Health Perspect. 2004 Apr;112(5):631-5 [15064173] J Natl Cancer Inst. 2004 Sep 15;96(18):1375-82 [15367570] Am J Epidemiol. 2004 Feb 15;159(4):373-80 [14769641] Occup Med. 1991 Jul-Sep;6(3):327-34 [1948522] Scand J Work Environ Health. 1992 Aug;18(4):209-15 [1411362] Am J Ind Med. 1997 Feb;31(2):233-42 [9028440] Environ Health Perspect. 1998 Jul;106(7):415-20 [9637799] Environ Res. 1999 Feb;80(2 Pt 1):172-9 [10092410] Ann Epidemiol. 2005 Apr;15(4):279-85 [15780775] J Expo Anal Environ Epidemiol. 2005 May;15(3):225-33 [15280893] Am J Respir Crit Care Med. 2002 Mar 1;165(5):683-9 [11874814] Epidemiology. 2002 Jan;13(1):94-9 [11805592] Am J Ind Med. 2000 Jun;37(6):618-28 [10797505] J Agric Saf Health. 2003 Feb;9(1):5-18 [12673912] J Expo Anal Environ Epidemiol. 2002 Nov;12(6):418-26 [12415490] Ann Occup Hyg. 2002 Mar;46(2):245-60 [12074034] Am J Epidemiol. 2004 Nov 1;160(9):876-85 [15496540] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Two C-methyl derivatives of [11C]WAY-100635--effects of an amido alpha-methyl group on metabolism and brain 5-HT1A receptor radioligand behavior in monkey. AN - 67865927; 15912425 AB - [carbonyl-11C]N-(2-(1-(4-(2-methoxyphenyl)-piperazinyl)ethyl)-N-pyridinyl)cyclohexanecarboxamide ([carbonyl-11C]WAY-100635) is an effective radioligand for imaging brain 5-HT1A receptors with positron emission tomography (PET). However, this radioligand has some drawbacks for deriving relative regional receptor densities, including rapid metabolism, which acts against accurate definition of an arterial input function for compartmental modeling, and very low nonspecific binding in brain, which detracts from the accuracy of modeling by a simplified reference tissue (cerebellum) approach. Here, in a search for a radioligand that overcomes these limitations, we investigated the effects of introducing a single methyl group at either of the carbon atoms alpha to the amide bond in [11C]WAY-100635. Ligands with a methyl group on the alpha carbon of the cyclohexyl group (SWAY) or the alpha carbon of the C2H4 linker ((R,S)-JWAY) were synthesized and tested for binding affinity and intrinsic activity at 5-HT1A receptors. SWAY was labeled with carbon-11 (t1/2 = 20.4 minutes; beta+ = 99.8%) in its O-methyl group and (R,S)-JWAY in its carbonyl group. Each radioligand was evaluated by PET experiments in cynomolgus monkey. SWAY and (R,S)-JWAY were found to be high-affinity antagonists at 5-HT1A receptors. After injection of [11C]SWAY into monkey, radioactivity uptake in brain reached a maximum of 3% at 4.5 minutes and decreased to 0.7% at 72 minutes. However, over the time span of the experiment, radioactivity concentrations in 5-HT1A receptor-rich brain regions were only fractionally higher than in cerebellum. Radioactivity represented by parent radioligand in plasma was 39% at 45 minutes. After injection of [11C](R,S)-JWAY alone, radioactivity uptake in brain reached a maximum of 4.8% at 2.5 minutes and decreased to 1.2% at 90 minutes. At this time, radioactivity concentration in 5-HT1A receptor-rich brain regions was markedly greater than in cerebellum. In another PET experiment, the monkey was predosed with WAY-100635 before [11C](R,S)-JWAY injection. At 90 minutes after injection, the ratio of radioactivity in 5-HT1A receptor-rich regions to that in cerebellum was reduced to near unity. Radioactivity represented by parent radioligand in plasma was 12% at 45 minutes. [11C](R,S)-JWAY, but not [11C]SWAY, gives a sizeable 5-HT1A receptor-selective PET signal in monkey. The presence of a C-methyl group adjacent to the amide bond in SWAY or (R,S)-JWAY fails to counter metabolism. JF - Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging AU - McCarron, Julie A AU - Marchais-Oberwinkler, Sandrine AU - Pike, Victor W AU - Tarkiainen, Jari AU - Halldin, Christer AU - Sóvagó, Judit AU - Gulyas, Balàzs AU - Wikström, Hakan V AU - Farde, Lars AD - MRC Cyclotron Unit, Imperial College School of Medicine, Hammersmith Hospital, Ducane Road, London, W12 0NN, UK. mccarronj@intra.nimh.nih.gov PY - 2005 SP - 209 EP - 219 VL - 7 IS - 3 SN - 1536-1632, 1536-1632 KW - Carbon Radioisotopes KW - 0 KW - Ligands KW - Lipids KW - Piperazines KW - Pyridines KW - Receptors, Serotonin, 5-HT1 KW - Serotonin 5-HT1 Receptor Antagonists KW - N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide KW - 71IH826FEG KW - Index Medicus KW - Radiochemistry KW - Molecular Structure KW - Hydrophobic and Hydrophilic Interactions KW - Animals KW - Positron-Emission Tomography KW - Lipids -- chemistry KW - Inhibitory Concentration 50 KW - Methylation KW - Carbon Radioisotopes -- chemistry KW - Amination KW - Receptors, Serotonin, 5-HT1 -- metabolism KW - Pyridines -- chemistry KW - Piperazines -- chemistry KW - Brain -- drug effects KW - Pyridines -- pharmacokinetics KW - Brain -- metabolism KW - Radioligand Assay KW - Haplorhini -- metabolism KW - Piperazines -- pharmacokinetics KW - Piperazines -- chemical synthesis KW - Pyridines -- chemical synthesis KW - Pyridines -- blood KW - Piperazines -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67865927?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Validation+status+of+the+hens+egg+test-chorioallantoic+membrane+%28HET-CAM%29+test+method&rft.au=Choksi%2C+N%3BAllen%2C+D%3BInhof%2C+C%3BTruax%2C+J%3BTice%2C+R%3BStokes%2C+W&rft.aulast=Choksi&rft.aufirst=N&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Methadone for cancer pain: what have we learned from clinical studies? AN - 67860460; 15909785 AB - The analgesic ladder guideline proposed by the World Health Organization has been shown to be effective in controlling cancer pain in about 80 percent of patients, but the remaining 20 percent still experience pain. Several strategies have been used to manage refractory cancer pain and opioid toxicity. Switching opioids, alternative routes of opioid administration, optimizing adjuvants, and invasive procedures are proposed treatments. Extensive medical literature corroborates each one of those treatments. Rotation from one opioid to another is a noninvasive strategy to overcome opioid side effects and refractory pain. Frequently, methadone is used during opioid rotation. However, there is a lack of consensus on how to proceed on rotation from morphine to methadone. In the current era of evidence-based medicine, the medical literature fails to answer some cancer pain-management issues. The purpose of this review is to clarify a process for transitioning from morphine to methadone. JF - The American journal of hospice & palliative care AU - Soares, Luiz Guilherme L AD - Pain Clinic, National Cancer Institute, Rio de Janeiro, Brazil. PY - 2005 SP - 223 EP - 227 VL - 22 IS - 3 SN - 1049-9091, 1049-9091 KW - Analgesics, Opioid KW - 0 KW - Morphine KW - 76I7G6D29C KW - Methadone KW - UC6VBE7V1Z KW - Nursing KW - World Health Organization KW - Morphine -- therapeutic use KW - Dose-Response Relationship, Drug KW - Humans KW - Pain Measurement KW - Pain -- etiology KW - Methadone -- adverse effects KW - Pain -- drug therapy KW - Neoplasms -- complications KW - Methadone -- therapeutic use KW - Analgesics, Opioid -- therapeutic use KW - Methadone -- administration & dosage KW - Analgesics, Opioid -- adverse effects KW - Analgesics, Opioid -- administration & dosage KW - Palliative Care -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67860460?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+hospice+%26+palliative+care&rft.atitle=Methadone+for+cancer+pain%3A+what+have+we+learned+from+clinical+studies%3F&rft.au=Soares%2C+Luiz+Guilherme+L&rft.aulast=Soares&rft.aufirst=Luiz+Guilherme&rft.date=2005-05-01&rft.volume=22&rft.issue=3&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+hospice+%26+palliative+care&rft.issn=10499091&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Am J Hosp Palliat Care. 2005 Sep-Oct;22(5):337 [16225353] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - VEGF contributes to mammary tumor growth in transgenic mice through paracrine and autocrine mechanisms. AN - 67850508; 15765121 AB - Vascular endothelial growth factor (VEGF) has been identified as a vascular permeability factor, angiogenic cytokine, and a survival factor. To address its role in mammary carcinogenesis, we used transgenic mice with human VEGF(165) targeted to mammary epithelial cells under the control of the mouse mammary tumor virus (MMTV) promoter. Metastatic mammary carcinomas were induced by mating the MMTV-VEGF mice with MMTV-polyoma virus middle T-antigen (MT) mice to generate VEGF/MT mice. Tumor latency was decreased in the VEGF/MT mice, which developed mammary carcinomas with increased vasodilatation at 4 weeks of age. There was increased incidence, multiplicity, and weight of the mammary tumors in 6- and 8-week-old VEGF/MT mice, compared to their MT-only littermates. Macro- and microscopic lung metastases were detected in the VEGF/MT mice but not the MT mice at 6 and 8 weeks of age. Enhanced tumor growth was attributed to increased microvascular density (MVD), as well as increased tumor cell proliferation and survival. Angiogenesis array analysis showed that 24 of 25 differentially expressed genes were upregulated in the VEGF/MT tumors. In vitro studies revealed increased proliferative activity and upregulation of Flk-1 in the VEGF/MT tumor cells, compared with the MT-only tumor cells. Moreover, there was decreased proliferative activity with downregulation of Flk-1 in tumor cells isolated from conditional knockout (VEGF(-/-)) MT-induced mammary carcinomas. The slow growing VEGF(-/-) tumor cells were accumulated in the G(1)/G(0) phase of the cell cycle and this was associated with stimulation of p16(ink4a) and p21(WAF1). Similarly, p16(ink4a) was stimulated in VEGF(lox/lox)/MT mammary tumor cells following Adeno-cre-mediated VEGF gene inactivation. Collectively, the data from these transgenic models indicate that VEGF contributes to mammary tumor growth through increased neovascularization, as well as autocrine stimulation of growth and inhibition of apoptosis. JF - Laboratory investigation; a journal of technical methods and pathology AU - Schoeffner, Daniel J AU - Matheny, Shannon L AU - Akahane, Takemi AU - Factor, Valentina AU - Berry, Adam AU - Merlino, Glenn AU - Thorgeirsson, Unnur P AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 608 EP - 623 VL - 85 IS - 5 SN - 0023-6837, 0023-6837 KW - Cdkn1a protein, mouse KW - 0 KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - RNA, Messenger KW - RNA, Neoplasm KW - Vascular Endothelial Growth Factor A KW - vascular endothelial growth factor A, mouse KW - Vascular Endothelial Growth Factor Receptor-2 KW - EC 2.7.10.1 KW - Index Medicus KW - Animals KW - Blotting, Northern KW - Oligonucleotide Array Sequence Analysis KW - Lung Neoplasms -- secondary KW - Vascular Endothelial Growth Factor Receptor-2 -- genetics KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Neoplasm -- analysis KW - Mice, Transgenic KW - Cell Proliferation KW - Genes, p16 KW - Mice, Knockout KW - Apoptosis -- genetics KW - RNA, Messenger -- metabolism KW - Cell Cycle Proteins -- genetics KW - Lung Neoplasms -- genetics KW - Up-Regulation KW - Vascular Endothelial Growth Factor Receptor-2 -- metabolism KW - Immunohistochemistry KW - Male KW - Female KW - Lung Neoplasms -- metabolism KW - Adenocarcinoma -- metabolism KW - Mammary Neoplasms, Animal -- pathology KW - Mammary Neoplasms, Animal -- genetics KW - Adenocarcinoma -- secondary KW - Mammary Neoplasms, Animal -- metabolism KW - Adenocarcinoma -- genetics KW - Vascular Endothelial Growth Factor A -- genetics KW - Vascular Endothelial Growth Factor A -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67850508?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Aberrant+gene+expression+in+the+neonatal+mouse+lung+following+in+utero+exposure+to+inorganic+arsenic&rft.au=Shen%2C+J%3BXie%2C+Y%3BLiu%2C+J%3BDiwan%2C+B%3BWaalkes%2C+M&rft.aulast=Shen&rft.aufirst=J&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-05-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevalence and motives for illicit use of prescription stimulants in an undergraduate student sample. AN - 67844360; 15900989 AB - To assess the prevalence and motives for illicit use of prescription stimulants and alcohol and other drugs (AODs), associated with these motives, the authors distributed a self-administered Web survey TO a random sample of 9,161 undergraduate college students. Of the study participants, 8.1% reported lifetime and 5.4% reported past-year illicit use of prescription stimulants. The most prevalent motives given for illicit use of prescription stimulants were to (1) help with concentration, (2) increase alertness, and (3) provide a high. Although men were more likely than women were to report illicit use of prescription stimulants, the authors found no gender differences in motives. Regardless of motive, illicit use of prescription stimulants was associated with elevated rates of AOD use, and number of motives endorsed and AOD use were positively related. Students appear to be using these prescription drugs non-medically, mainly to enhance performance or get high. JF - Journal of American college health : J of ACH AU - Teter, Christian J AU - McCabe, Sean Esteban AU - Cranford, James A AU - Boyd, Carol J AU - Guthrie, Sally K AD - National Institute on Drug Abuse, College of Pharmacy and Substance Abuse Research Center at the University of Michigan, Ann Arbor, USA. c.teter@neu.edu PY - 2005 SP - 253 EP - 262 VL - 53 IS - 6 SN - 0744-8481, 0744-8481 KW - Central Nervous System Stimulants KW - 0 KW - Street Drugs KW - Index Medicus KW - Motivation KW - Attitude to Health KW - Risk Factors KW - Humans KW - Chi-Square Distribution KW - Surveys and Questionnaires KW - United States -- epidemiology KW - Male KW - Internet KW - Female KW - Prevalence KW - Students -- psychology KW - Self Disclosure KW - Students -- statistics & numerical data KW - Central Nervous System Stimulants -- administration & dosage KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67844360?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Gingival+carcinogenicity+in+female+Harlan+Sprague-Dawley+rats+after+oral+treatment+for+two+years+with+2%2C+3%2C+7%2C+8-tetrachlorodibenzo-p-dioxin+and+dioxin-like+compounds&rft.au=Yoshizawa%2C+K%3BWalker%2C+N+J%3BJokinen%2C+M+P%3BBrix%2C+A+E%3BSells%2C+D+M%3BMarsh%2C+T%3BWyde%2C+ME%3BOrzech%2C+D%3BHaseman%2C+J+K%3BNyska%2C+A&rft.aulast=Yoshizawa&rft.aufirst=K&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tumor vaccine: current trends in antigen specific immunotherapy. AN - 67840988; 15900903 AB - Effective cancer treatment to prevent the tumor growth as well as to stop its recurrence is the dream of oncologists. Currently available therapeutic measures like, radiotherapy and chemotherapy, often suffer from severe toxicity and lack of specificity of the drug towards tumor cells. Another promising approach is the 'immunotherapy', in which either the immune system is activated by tumor vaccine to combat the tumor growth or antitumor antibodies can be used. Vaccination can stimulate humoral, cellular and innate immune systems to generate various effector molecules, like antibody, cytotoxic T cells, cytokines etc. In antigen specific immunotherapy, the immune system can be stimulated actively by antigen based tumor vaccine to kill only those tumor cells, having expression of the particular tumor associated antigen. Different experimental, preclinical and clinical studies have proved that generated immune responses are effective to restrict the tumor growth. Useful strategies of antigen specific immunotherapy and outcome of various laboratory and clinic based studies are discussed. JF - Indian journal of experimental biology AU - Baral, Rathindranath AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata 700 026, India. rbaral@hotmail.com Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 389 EP - 406 VL - 43 IS - 5 SN - 0019-5189, 0019-5189 KW - Antigens, Neoplasm KW - 0 KW - Cancer Vaccines KW - Index Medicus KW - Humans KW - T-Lymphocytes -- immunology KW - Cancer Vaccines -- immunology KW - Immunotherapy KW - Cancer Vaccines -- therapeutic use KW - Neoplasms -- therapy KW - Antigens, Neoplasm -- immunology KW - Neoplasms -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67840988?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Indian+journal+of+experimental+biology&rft.atitle=Tumor+vaccine%3A+current+trends+in+antigen+specific+immunotherapy.&rft.au=Baral%2C+Rathindranath&rft.aulast=Baral&rft.aufirst=Rathindranath&rft.date=2005-05-01&rft.volume=43&rft.issue=5&rft.spage=389&rft.isbn=&rft.btitle=&rft.title=Indian+journal+of+experimental+biology&rft.issn=00195189&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Quantifying the risks associated with exceeding recommended drinking limits. AN - 67834313; 15897737 AB - Although daily and weekly drinking limits demonstrate strong sensitivity and specificity in identifying alcohol use disorders (AUDs), there are no descriptive data that present the risks associated with exceeding these limits in a format suitable for presentation to patients, students, and the general public. Data collected in the 2001-2002 National Epidemiologic Survey on Alcohol and Related Conditions were used to estimate the risks of past-year DSM-IV alcohol abuse and dependence associated with various frequencies of exceeding daily drinking limits (no more than 4 drinks for men; no more than 3 drinks for women) in a nationally representative sample of 26,946 US drinkers 18 years of age and older. These risks were further categorized by whether weekly drinking limits (no more than 14 drinks for men; no more than 7 drinks for women) were exceeded and by maximum number of drinks consumed in the past year. The prevalence of alcohol dependence with abuse increased in a fairly linear fashion with frequency of exceeding daily drinking limits. The prevalence of dependence alone (no abuse) and abuse alone (no dependence) peaked among persons who exceeded the daily limits twice a week and then leveled off, because individuals became increasingly likely to have both disorders at higher frequencies. Exceeding the weekly limits generally increased the risks of both disorders after accounting for frequency of exceeding the daily limits, but not always to a significant extent. Likewise, maximum quantity of drinks consumed was positively associated with the risks of AUDs even after accounting for frequency of risk drinking. There were few gender differences in the risk of dependence after adjusting for frequency of exceeding daily drinking limits, but the risk of alcohol abuse remained greater among men. These data provide a useful tool for illustrating the broad range of risk of AUDs associated with exceeding recommended drinking limits, and they support the utility of the daily and weekly drinking limits in predicting AUDs. JF - Alcoholism, clinical and experimental research AU - Dawson, Deborah A AU - Grant, Bridget F AU - Li, Ting-Kai AD - Laboratory of Epidemiology and Biometry Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892-9304, USA. ddawson@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 902 EP - 908 VL - 29 IS - 5 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Psychiatric Status Rating Scales KW - Sex Factors KW - Humans KW - Adult KW - United States -- epidemiology KW - Male KW - Female KW - Risk Assessment KW - Alcoholism -- epidemiology KW - Alcohol Drinking -- psychology KW - Alcohol Drinking -- epidemiology KW - Alcoholism -- psychology KW - Alcoholism -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67834313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Quantifying+the+risks+associated+with+exceeding+recommended+drinking+limits.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BLi%2C+Ting-Kai&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-05-01&rft.volume=29&rft.issue=5&rft.spage=902&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The anti-invasive activity of cyclooxygenase inhibitors is regulated by the transcription factor ATF3 (activating transcription factor 3). AN - 67833343; 15897233 AB - We previously showed that nonsteroidal anti-inflammatory drugs (NSAID) such as sulindac sulfide, which has chemopreventive activity, modulate the expression of several genes detected by microarray analysis. Activating transcription factor 3 (ATF3) was selected for further study because it is a transcription factor involved in cell proliferation, apoptosis, and invasion, and its expression is repressed in human colorectal tumors as compared with normal adjacent tissue. In this report, we show that ATF3 mRNA and protein expression are up-regulated in HCT-116 human colorectal cancer cells following treatment with NSAIDs, troglitazone, diallyl disulfide, and resveratrol. To ascertain the biological significance of ATF3, we overexpressed full-length ATF3 protein in the sense and antisense orientations. Overexpression of ATF3 in the sense orientation decreased focus formation in vitro and reduced the size of mouse tumor xenografts by 54% in vivo. Conversely, overexpression of antisense ATF3 was protumorigenic in vitro, however, not in vivo. ATF3 in the sense orientation did not modulate apoptosis, indicating another mechanism is involved. With microarray analysis, several genes relating to invasion and metastasis were identified by ATF3 overexpression and were confirmed by real-time reverse transcription-PCR, and several of these genes were modulated by sulindac sulfide, which inhibited invasion in these cells. Furthermore, overexpression of ATF3 inhibited invasion to a similar degree as sulindac sulfide treatment, whereas antisense ATF3 increased invasion. In conclusion, ATF3 represents a novel mechanism in which NSAIDs exert their anti-invasive activity, thereby linking ATF3 and its gene regulatory activity to the biological activity of these compounds. JF - Molecular cancer therapeutics AU - Bottone, Frank G AU - Moon, Yuseok AU - Kim, Jong Sik AU - Alston-Mills, Brenda AU - Ishibashi, Minako AU - Eling, Thomas E AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, P.O. Box 12233, 111 T.W. Alexander Drive, Research Triangle Park, NC 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 693 EP - 703 VL - 4 IS - 5 SN - 1535-7163, 1535-7163 KW - Activating Transcription Factor 3 KW - 0 KW - Allyl Compounds KW - Anti-Inflammatory Agents, Non-Steroidal KW - Chromans KW - Cyclooxygenase Inhibitors KW - Disulfides KW - RNA, Messenger KW - Stilbenes KW - Thiazolidinediones KW - Transcription Factors KW - Sulindac KW - 184SNS8VUH KW - diallyl disulfide KW - 5HI47O6OA7 KW - sulindac sulfide KW - 6UVA8S2DEY KW - troglitazone KW - I66ZZ0ZN0E KW - resveratrol KW - Q369O8926L KW - Index Medicus KW - Animals KW - Stilbenes -- pharmacology KW - Disulfides -- pharmacology KW - Humans KW - Allyl Compounds -- pharmacology KW - Mice, Nude KW - Mice KW - HCT116 Cells KW - RNA, Messenger -- genetics KW - Gene Expression Regulation, Neoplastic -- drug effects KW - RNA, Messenger -- metabolism KW - Chromans -- pharmacology KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Apoptosis -- drug effects KW - Thiazolidinediones -- pharmacology KW - Microarray Analysis KW - Transplantation, Heterologous KW - Up-Regulation KW - Male KW - Transcription Factors -- metabolism KW - Neoplasm Invasiveness -- pathology KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- metabolism KW - Sulindac -- pharmacology KW - Sulindac -- analogs & derivatives KW - Transcription Factors -- genetics KW - Cyclooxygenase Inhibitors -- pharmacology KW - Anti-Inflammatory Agents, Non-Steroidal -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67833343?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+therapeutics&rft.atitle=The+anti-invasive+activity+of+cyclooxygenase+inhibitors+is+regulated+by+the+transcription+factor+ATF3+%28activating+transcription+factor+3%29.&rft.au=Bottone%2C+Frank+G%3BMoon%2C+Yuseok%3BKim%2C+Jong+Sik%3BAlston-Mills%2C+Brenda%3BIshibashi%2C+Minako%3BEling%2C+Thomas+E&rft.aulast=Bottone&rft.aufirst=Frank&rft.date=2005-05-01&rft.volume=4&rft.issue=5&rft.spage=693&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-09 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A correlation study of organochlorine levels in serum, breast adipose tissue, and gluteal adipose tissue among breast cancer cases in India. AN - 67833200; 15894661 AB - We used data from a breast cancer pilot study carried out in Kerala, India in 1997, for which organochlorine levels were measured in three biological media, blood serum, breast adipose tissue, and gluteal adipose tissue, of 37 fasting breast cancer cases (pretreatment). Our objective was to investigate the relationships between organochlorine concentrations in different biological media. Gas-liquid chromatography determined serum, breast adipose, and gluteal adipose tissue levels of dichlorodiphenyltricholorethane, dichlorodiphenyldichloroethane, beta-benzene hexachloride, and polychlorinated biphenyl (PCB) congeners, PCB-153 and PCB-180. Correlation plots were made and Spearman correlation coefficients (r) calculated for breast adipose tissue versus serum, gluteal adipose tissue versus serum, and breast adipose versus gluteal adipose tissue. We also examined paired ratios of all summary statistics. There were strong correlations among serum, breast adipose tissue, and gluteal adipose tissue concentrations for most organochlorines analyzed, one exception being gluteal versus serum for PCB-153. The correlations for all other comparisons ranged from r = 0.65 to 0.94. Serum (ng/g) versus adipose ratios approached 1:1 for most of the organochlorine pesticide comparisons and did not vary by summary statistic. To our knowledge, this is the first study to use three different media from fasting subjects and to comprehensively investigate the relationship between organochlorines measured across the three media for both organochlorine pesticides and PCBs. These data indicate that blood serum reflects the present body burden of a range of organochlorines to the same extent as adipose tissue, and they support the view that serum may be collected in lieu of adipose tissue to obtain similar information. However, such measurements are a combination of both recent exposures and past exposures, which have metabolized slowly and may still persist. Therefore, investigators should use caution when assigning a level as lifetime body burden. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Rusiecki, Jennifer A AU - Matthews, Aleyama AU - Sturgeon, Susan AU - Sinha, Rashmi AU - Pellizzari, Edo AU - Zheng, Tongzhang AU - Baris, Dalsu AD - Occupational and Environmental Epidemiology Branch, National Cancer Institute, 6120 Executive Boulevard, EPS 8111, Bethesda, MD 20892-7240, USA. rusieckj@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1113 EP - 1124 VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - Environmental Pollutants KW - 0 KW - Hydrocarbons, Chlorinated KW - Insecticides KW - Pesticide Residues KW - Index Medicus KW - Age Factors KW - Chromatography, Gas KW - Humans KW - Aged KW - Pilot Projects KW - Environmental Pollutants -- analysis KW - Environmental Pollutants -- pharmacokinetics KW - India KW - Buttocks KW - Adult KW - Breast -- chemistry KW - Middle Aged KW - Statistics as Topic KW - Female KW - Insecticides -- metabolism KW - Hydrocarbons, Chlorinated -- blood KW - Hydrocarbons, Chlorinated -- analysis KW - Pesticide Residues -- blood KW - Hydrocarbons, Chlorinated -- metabolism KW - Breast Neoplasms -- chemistry KW - Pesticide Residues -- analysis KW - Pesticide Residues -- metabolism KW - Insecticides -- analysis KW - Adipose Tissue -- chemistry KW - Insecticides -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67833200?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=A+correlation+study+of+organochlorine+levels+in+serum%2C+breast+adipose+tissue%2C+and+gluteal+adipose+tissue+among+breast+cancer+cases+in+India.&rft.au=Rusiecki%2C+Jennifer+A%3BMatthews%2C+Aleyama%3BSturgeon%2C+Susan%3BSinha%2C+Rashmi%3BPellizzari%2C+Edo%3BZheng%2C+Tongzhang%3BBaris%2C+Dalsu&rft.aulast=Rusiecki&rft.aufirst=Jennifer&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1113&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Aspirin use and risk of biliary tract cancer: a population-based study in Shanghai, China. AN - 67833038; 15894693 AB - The association of gallbladder and bile duct cancers with gallstones, cholecystitis, and cholangitis suggest that chronic inflammation contributes to the carcinogenic process. However, the effect of nonsteroidal anti-inflammatory drugs, such as aspirin, on biliary tract cancer has not been well studied. In a population-based case-control study conducted in Shanghai, China, we examined the relationship between aspirin use and the risk of biliary disease. A total of 627 patients with biliary tract cancer, including cancers of the gallbladder (n = 368), extrahepatic bile duct (n = 191), and ampulla of Vater (n = 68); 1,037 patients with biliary stones; and 958 healthy adults were included in the study. Self-reported data on aspirin use was collected from study participants by in-person interview. The prevalence of aspirin use was low, with 5.7% of the population controls being regular users. After controlling for age, sex, education, and biliary stone status, aspirin use was associated with a reduced risk of gallbladder cancer [odds ratio (OR), 0.37; 95% confidence interval (CI), 0.17-0.88]. An inverse relationship was also observed for frequency and duration of use and with younger age when starting use. In addition, there was a nonsignificant reduction in the risk of bile duct (OR, 0.48; 95% CI, 0.19-1.19) and ampullary cancers (OR, 0.22; 95% CI, 0.03-1.65) associated with aspirin use, whereas no clear association was seen with biliary stones (OR, 0.92; 95% CI, 0.59-1.44). Further studies of biliary tract cancer in other populations are needed to confirm these results and to elucidate the mechanisms that underlie the reduced risk associated with use of aspirin and possibly other nonsteroidal anti-inflammatory drugs. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Liu, Enju AU - Sakoda, Lori C AU - Gao, Yu-Tang AU - Rashid, Asif AU - Shen, Ming-Chang AU - Wang, Bing-Sheng AU - Deng, Jie AU - Han, Tian-Quan AU - Zhang, Bai-He AU - Fraumeni, Joseph F AU - Hsing, Ann W AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Department of Health and Human Services, 6120 Executive Boulevard, EPS 7058, Bethesda, MD 20892-7234, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1315 EP - 1318 VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - Anti-Inflammatory Agents, Non-Steroidal KW - 0 KW - Aspirin KW - R16CO5Y76E KW - Index Medicus KW - Sex Factors KW - Humans KW - Aged KW - Gallstones -- diagnosis KW - Gallstones -- complications KW - Risk Factors KW - China -- epidemiology KW - Adult KW - Case-Control Studies KW - Interviews as Topic KW - Middle Aged KW - Chemoprevention KW - Gallstones -- surgery KW - Female KW - Male KW - Biliary Tract Neoplasms -- epidemiology KW - Anti-Inflammatory Agents, Non-Steroidal -- therapeutic use KW - Aspirin -- administration & dosage KW - Aspirin -- therapeutic use KW - Biliary Tract Neoplasms -- prevention & control KW - Biliary Tract Neoplasms -- complications KW - Anti-Inflammatory Agents, Non-Steroidal -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67833038?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Aspirin+use+and+risk+of+biliary+tract+cancer%3A+a+population-based+study+in+Shanghai%2C+China.&rft.au=Liu%2C+Enju%3BSakoda%2C+Lori+C%3BGao%2C+Yu-Tang%3BRashid%2C+Asif%3BShen%2C+Ming-Chang%3BWang%2C+Bing-Sheng%3BDeng%2C+Jie%3BHan%2C+Tian-Quan%3BZhang%2C+Bai-He%3BFraumeni%2C+Joseph+F%3BHsing%2C+Ann+W&rft.aulast=Liu&rft.aufirst=Enju&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1315&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Smoking is a risk factor for cervical intraepithelial neoplasia grade 3 among oncogenic human papillomavirus DNA-positive women with equivocal or mildly abnormal cytology. AN - 67832896; 15894667 AB - Smoking is a potential risk factor for cervical cancer and its immediate precursor, cervical intraepithelial neoplasia grade 3 (CIN3), but few studies have adequately taken into account the possible confounding effect of oncogenic human papillomavirus (HPV) infection. Women (n = 5,060) with minimally abnormal Papanicolaou smears were enrolled in the ASCUS and LSIL Triage Study, a clinical trial to evaluate management strategies, and were seen every 6 months for the 2-year duration of the study. Cervical specimens were tested for HPV DNA using both Hybrid Capture 2 and PGMY09/11 L1 consensus primer PCR with reverse line blot hybridization for genotyping. Multivariate logistics regression models were used to assess associations [odds ratio (OR) with 95% confidence intervals (95% CI)] between smoking behaviors and rigorously reviewed cases of cervical intraepithelial neoplasia grade 3 or cancer (> or =CIN3) identified throughout the study (n = 506) in women with oncogenic HPV (n = 3,133). Current smoking was only weakly associated with increased HPV infection. Among infected women, current smokers (OR, 1.7; 95% CI, 1.4-2.1) and past smokers (OR, 1.7; 95% CI, 1.2-2.4) were more likely to be diagnosed with > or =CIN3 than nonsmokers. Greater smoking intensity (P(Trend) or =2 packs/d (OR, 3.3; 95% CI, 1.5-7.5) and smoking for > or =11 years (OR, 2.1; 95% CI, 1.5-2.9) most strongly associated with > or =CIN3 as compared to non-smokers. The effects of intensity and duration seemed additive. Women with oncogenic HPV and minimally abnormal Papanicolaou smears who smoke were up to three times more likely to be diagnosed with > or =CIN3 than nonsmokers. Smoking cessation trials targeting this population might be warranted. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - McIntyre-Seltman, Kathleen AU - Castle, Philip E AU - Guido, Richard AU - Schiffman, Mark AU - Wheeler, Cosette M AU - ALTS Group AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Room 7074, 6120 Executive Boulevard, EPS MSC 7234, Bethesda, MD 20892-7234, USA. ; ALTS Group Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1165 EP - 1170 VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - DNA, Viral KW - 0 KW - Nicotine KW - 6M3C89ZY6R KW - Index Medicus KW - Neoplasm Staging KW - Nicotine -- toxicity KW - Humans KW - Aged KW - Vaginal Smears -- methods KW - Genotype KW - Polymerase Chain Reaction KW - Prospective Studies KW - Oncogenes KW - Triage KW - Aged, 80 and over KW - Logistic Models KW - Risk Factors KW - Adult KW - Middle Aged KW - Adolescent KW - DNA, Viral -- genetics KW - Female KW - Papanicolaou Test KW - Papillomavirus Infections -- epidemiology KW - Papillomaviridae -- classification KW - Uterine Cervical Neoplasms -- epidemiology KW - Papillomavirus Infections -- complications KW - Smoking -- adverse effects KW - Cervical Intraepithelial Neoplasia -- virology KW - Papillomaviridae -- genetics KW - Smoking -- epidemiology KW - Uterine Cervical Neoplasms -- virology KW - Papillomaviridae -- drug effects KW - Cervical Intraepithelial Neoplasia -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67832896?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Smoking+is+a+risk+factor+for+cervical+intraepithelial+neoplasia+grade+3+among+oncogenic+human+papillomavirus+DNA-positive+women+with+equivocal+or+mildly+abnormal+cytology.&rft.au=McIntyre-Seltman%2C+Kathleen%3BCastle%2C+Philip+E%3BGuido%2C+Richard%3BSchiffman%2C+Mark%3BWheeler%2C+Cosette+M%3BALTS+Group&rft.aulast=McIntyre-Seltman&rft.aufirst=Kathleen&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1165&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effectiveness of the derived Alcohol Use Disorders Identification Test (AUDIT-C) in screening for alcohol use disorders and risk drinking in the US general population. AN - 67829981; 15897730 AB - The three consumption questions from the Alcohol Use Disorders Identification Test (AUDIT-C) are increasingly used as a screener for alcohol use disorders (AUDs) and risk drinking. In a representative sample of US adults 18 years of age and older, AUDIT-C scores (derived from consumption questions embedded in a large national survey) were used to estimate sensitivity, specificity, and areas under receiver operator characteristic curves (AUROCs) for alcohol dependence, any AUD, and risk drinking. AUDs were defined according to DSM-IV criteria. For men, risk drinking was defined as consuming >14 drinks per week or >4 drinks in a single day at least once a month; for women, the weekly and daily limits were >7 drinks and >3 drinks, respectively. The derived AUDIT-C was evaluated among past-year drinkers (n = 26,946), within the total population (n = 43,093), in groups defined by age, sex, and race/ethnicity, and among pregnant women, persons attending an emergency room, and college students. For past-year drinkers, the AUROCs for the derived AUDIT-C were 0.887 for alcohol dependence, 0.860 for any AUD, and 0.966 for risk drinking. Scores were higher in the total population, 0.931, 0.917, and 0.981, respectively. The derived AUDIT-C performed slightly better in screening for dependence among women than men. Screening for risk drinking was better among men, probably because the third AUDIT-C question directly mirrors one of the definitions of risk drinking for men but not for women. Performance in pregnant women, past-year emergency room patients, and college students was on a par with performance in the general population. The derived AUDIT-C performs well in screening for AUDs and risk drinking. The use of variable cut points for men and women improves its sensitivity and specificity. Validation in a realistic screening situation, in which the AUDIT-C questions are asked as stand-alone and not embedded items, is a critical future step. JF - Alcoholism, clinical and experimental research AU - Dawson, Deborah A AU - Grant, Bridget F AU - Stinson, Frederick S AU - Zhou, Yuan AD - Laboratory of Epidemiology and Biometry, Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892-9304, USA. ddawson@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 844 EP - 854 VL - 29 IS - 5 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Age Factors KW - Risk-Taking KW - ROC Curve KW - Humans KW - Aged KW - Pregnancy KW - Psychiatric Status Rating Scales KW - Ethnic Groups KW - Adult KW - Middle Aged KW - Adolescent KW - United States -- epidemiology KW - Female KW - Male KW - Alcoholism -- epidemiology KW - Alcoholism -- diagnosis KW - Alcohol Drinking -- psychology KW - Alcohol Drinking -- epidemiology KW - Alcoholism -- psychology KW - Psychological Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829981?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Histone+modifications+in+enhancer+activation+and+enhancer+blocking&rft.au=Dean%2C+A&rft.aulast=Dean&rft.aufirst=A&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Factor analysis of pesticide use patterns among pesticide applicators in the Agricultural Health Study. AN - 67821697; 15280893 AB - Exposure to certain pesticides has been linked with both acute and chronic adverse health outcomes such as neurotoxicity and risk for certain cancers. Univariate analyses of pesticide exposures may not capture the complexity of these exposures since use of various pesticides often occurs simultaneously, and because specific uses have changed over time. Using data from the Agricultural Health Study, a cohort study of 89,658 licensed pesticide applicators and their spouses in Iowa and North Carolina, we employed factor analysis to order to characterize underlying patterns of self-reported exposures to 50 different pesticides. Factor analysis is a statistical method used to explain the relationships between several correlated variables by reducing them to a smaller number of conceptually meaningful, composite variables, known as factors. Three factors emerged for farmer applicators (N=45,074): (1) Iowa agriculture and herbicide use, (2) North Carolina agriculture and use of insecticides, fumigants and fungicides, and (3) older age and use of chlorinated pesticides. The patterns observed for spouses of farmers (N=17,488) were similar to those observed for the farmers themselves, whereas five factors emerged for commercial pesticide applicators (N=4,384): (1) herbicide use, (2) older age and use of chlorinated pesticides, (3) use of fungicides and residential pest treatments, (4) use of animal insecticides, and (5) use of fumigants. Pesticide exposures did not correlate with lifestyle characteristics such as race, smoking status or education. This heterogeneity in exposure patterns may be used to guide etiologic studies of health effects of farmers and other groups exposed to pesticides. JF - Journal of exposure analysis and environmental epidemiology AU - Samanic, Claudine AU - Hoppin, Jane A AU - Lubin, Jay H AU - Blair, Aaron AU - Alavanja, Michael C R AD - National Cancer Institute, Division of Cancer Epidemiology & Genetics, Bethesda, Maryland, USA. samanicc@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 225 EP - 233 VL - 15 IS - 3 SN - 1053-4245, 1053-4245 KW - Pesticides KW - 0 KW - Index Medicus KW - Life Style KW - Factor Analysis, Statistical KW - Age Factors KW - Humans KW - Cohort Studies KW - Adult KW - Middle Aged KW - Iowa KW - Male KW - Female KW - Spouses KW - Risk Assessment KW - Pesticides -- analysis KW - Occupational Exposure KW - Agriculture UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67821697?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+exposure+analysis+and+environmental+epidemiology&rft.atitle=Factor+analysis+of+pesticide+use+patterns+among+pesticide+applicators+in+the+Agricultural+Health+Study.&rft.au=Samanic%2C+Claudine%3BHoppin%2C+Jane+A%3BLubin%2C+Jay+H%3BBlair%2C+Aaron%3BAlavanja%2C+Michael+C+R&rft.aulast=Samanic&rft.aufirst=Claudine&rft.date=2005-05-01&rft.volume=15&rft.issue=3&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Journal+of+exposure+analysis+and+environmental+epidemiology&rft.issn=10534245&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-23 N1 - Date created - 2005-05-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The role of microglia in paraquat-induced dopaminergic neurotoxicity. AN - 67818527; 15890010 AB - The herbicide paraquat (PQ) has been implicated as a potential risk factor for the development of Parkinson's disease. In this study, PQ (0.5-1 microM) was shown to be selectively toxic to dopaminergic (DA) neurons through the activation of microglial NADPH oxidase and the generation of superoxide. Neuron-glia cultures exposed to PQ exhibited a decrease in DA uptake and a decline in the number of tyrosine hydroxylase-immunoreactive cells. The selectivity of PQ for DA neurons was confirmed when PQ failed to alter gamma-aminobutyric acid uptake in neuron-glia cultures. Microglia-depleted cultures exposed to 1 microM PQ failed to demonstrate a reduction in DA uptake, identifying microglia as the critical cell type mediating PQ neurotoxicity. Neuron-glia cultures treated with PQ failed to generate tumor necrosis factor-alpha and nitric oxide. However, microglia-enriched cultures exposed to PQ produced extracellular superoxide, supporting the notion that microglia are a source of PQ-derived oxidative stress. Neuron-glia cultures from NADPH oxidase-deficient (PHOX-/-) mice, which lack the functional catalytic subunit of NADPH oxidase and are unable to produce the respiratory burst, failed to show neurotoxicity in response to PQ, in contrast to PHOX+/+ mice. Here we report a novel mechanism of PQinduced oxidative stress, where at lower doses, the indirect insult generated from microglial NADPH oxidase is the essential factor mediating DA neurotoxicity. JF - Antioxidants & redox signaling AU - Wu, Xue-Fei AU - Block, Michelle L AU - Zhang, Wei AU - Qin, Liya AU - Wilson, Belinda AU - Zhang, Wan-Qin AU - Veronesi, Bellina AU - Hong, Jau-Shyong AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. PY - 2005 SP - 654 EP - 661 VL - 7 IS - 5-6 SN - 1523-0864, 1523-0864 KW - Superoxides KW - 11062-77-4 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Paraquat KW - PLG39H7695 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats KW - NADPH Oxidase -- metabolism KW - Animals KW - Superoxides -- metabolism KW - NADPH Oxidase -- deficiency KW - Cells, Cultured KW - Mice KW - Substrate Specificity KW - NADPH Oxidase -- genetics KW - Male KW - Female KW - Mice, Knockout KW - Microglia -- physiology KW - Microglia -- enzymology KW - Neurons -- metabolism KW - Neurons -- drug effects KW - Dopamine -- metabolism KW - Microglia -- drug effects KW - Paraquat -- toxicity KW - Neurons -- pathology KW - Microglia -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67818527?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antioxidants+%26+redox+signaling&rft.atitle=The+role+of+microglia+in+paraquat-induced+dopaminergic+neurotoxicity.&rft.au=Wu%2C+Xue-Fei%3BBlock%2C+Michelle+L%3BZhang%2C+Wei%3BQin%2C+Liya%3BWilson%2C+Belinda%3BZhang%2C+Wan-Qin%3BVeronesi%2C+Bellina%3BHong%2C+Jau-Shyong&rft.aulast=Wu&rft.aufirst=Xue-Fei&rft.date=2005-05-01&rft.volume=7&rft.issue=5-6&rft.spage=654&rft.isbn=&rft.btitle=&rft.title=Antioxidants+%26+redox+signaling&rft.issn=15230864&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Use of mycophenolate mofetil for chronic, refractory immune cytopenias in children with autoimmune lymphoproliferative syndrome. AN - 67805542; 15877736 AB - Autoimmune lymphoproliferative syndrome (ALPS) is a disorder of apoptosis associated most often with heritable FAS mutations leading to lymphadenopathy, hypersplenism and chronic refractory autoimmune cytopenias. Mycophenolate mofetil (MMF) was used to treat cytopenias in 13 ALPS patients aged 9 months to 17 years from a cohort of 118 children (aged < 18 years) and 82 adults. Twelve responded for a median follow-up of 49 weeks (range 38-240 weeks), defined by maintenance of adequate blood counts and reduction in dosage or cessation of other immunosuppressive agents. This preliminary experience suggests that MMF may spare steroid usage in patients with ALPS-associated cytopenias. JF - British journal of haematology AU - Rao, V Koneti AU - Dugan, Faith AU - Dale, Janet K AU - Davis, Joie AU - Tretler, Jean AU - Hurley, John K AU - Fleisher, Thomas AU - Puck, Jennifer AU - Straus, Stephen E AD - Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases/NIH, 10 Center Drive, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 534 EP - 538 VL - 129 IS - 4 SN - 0007-1048, 0007-1048 KW - Immunosuppressive Agents KW - 0 KW - Prodrugs KW - Mycophenolic Acid KW - HU9DX48N0T KW - Index Medicus KW - Thrombocytopenia -- drug therapy KW - Neutropenia -- immunology KW - Humans KW - Anemia, Hemolytic, Autoimmune -- drug therapy KW - Child KW - Leukocyte Count KW - Child, Preschool KW - Infant KW - Thrombocytopenia -- immunology KW - Pain -- chemically induced KW - Follow-Up Studies KW - Adolescent KW - Neutropenia -- drug therapy KW - Anemia, Hemolytic, Autoimmune -- immunology KW - Male KW - Lymphoproliferative Disorders -- drug therapy KW - Mycophenolic Acid -- analogs & derivatives KW - Mycophenolic Acid -- therapeutic use KW - Autoimmune Diseases -- drug therapy KW - Lymphoproliferative Disorders -- immunology KW - Mycophenolic Acid -- adverse effects KW - Immunosuppressive Agents -- therapeutic use KW - Immunosuppressive Agents -- adverse effects KW - Autoimmune Diseases -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67805542?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Signaling+pathways+for+DNA+damage+and+repair%2C+apoptosis+and+lymphoid+progenitor+cell+survival+are+dysregulated+by+N-acetyl-L-cysteine&rft.au=French%2C+JE%3BMartin%2C+K+R%3BKari%2C+F+W%3BBarrett%2C+J+C&rft.aulast=French&rft.aufirst=JE&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-14 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neoadjuvant accelerated chemotherapy followed by hyperfractionated radiation therapy in patients with operable, locally advanced head and neck carcinoma. AN - 67804680; 15878759 AB - A prospective phase II trial was carried out to evaluate an accelerated chemotherapy (CT) regimen followed by hyperfractionated radiation therapy (RT) in previously untreated Stage III-IV, operable (total laryngectomy), head and neck cancer patients. The current study evaluates overall survival, loco-regional control, organ preservation rates and toxicity. Between April 1997 and December 2002, 68 patients with advanced head and neck cancer were treated with 3 cycles of induction CT (cisplatin and 5-fluorouracil; days 1, 14, and 28) followed by hyperfractionated RT (7440 cGy/62 fractions). Sixty patients received the planned RT-CT treatment. Two months after the end of RT, 96% of patients had a clinical complete remission of the primary and 66% of the neck disease. At a median follow-up of 32 months, the 3-year overall and disease-free survival rates were 66% and 76%, respectively. Seven patients recurred on the primary site, 1 on the neck and 2 patients only had distant metastases. The organ preservation rate was 73%. Acute grade 3-4 mucositis occurred in 75% of patients and an 18% rate of CT-related cardiotoxicity was reported. The accelerated CT-RT regimen achieves a high rate of larynx preservation albeit with considerable toxicity. The current prospective clinical trial was approved by the Ethics Committee of the Centro di Riferimento Oncologico (C.R.O.) on May 27, 1996, # CRO-02-96. Written informed consent was required from all patients entering the study. JF - Oral oncology AU - Franchin, Giovanni AU - Vaccher, Emanuela AU - Gobitti, Carlo AU - Minatel, Emilio AU - Politi, Doriano AU - Talamini, Renato AU - Di Gennaro, Giampiero AU - Savignano, Gabriella AU - Trovò, Mauro G AU - Tirelli, Umberto AU - Barzan, Luigi AD - Department of Radiation Oncology, Centro di Riferimento Oncologico, National Cancer Institute, 33081 Aviano, Italy. gfranchin@cro.it Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 526 EP - 533 VL - 41 IS - 5 SN - 1368-8375, 1368-8375 KW - Granulocyte Colony-Stimulating Factor KW - 143011-72-7 KW - Cisplatin KW - Q20Q21Q62J KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Humans KW - Mouth Mucosa -- radiation effects KW - Stomatitis -- etiology KW - Aged KW - Radiotherapy -- adverse effects KW - Cisplatin -- administration & dosage KW - Fluorouracil -- administration & dosage KW - Prospective Studies KW - Adult KW - Treatment Outcome KW - Granulocyte Colony-Stimulating Factor -- administration & dosage KW - Middle Aged KW - Chemotherapy, Adjuvant KW - Female KW - Male KW - Survival Analysis KW - Radiation Injuries -- etiology KW - Head and Neck Neoplasms -- radiotherapy KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Head and Neck Neoplasms -- drug therapy KW - Carcinoma, Squamous Cell -- radiotherapy KW - Carcinoma, Squamous Cell -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67804680?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oral+oncology&rft.atitle=Neoadjuvant+accelerated+chemotherapy+followed+by+hyperfractionated+radiation+therapy+in+patients+with+operable%2C+locally+advanced+head+and+neck+carcinoma.&rft.au=Franchin%2C+Giovanni%3BVaccher%2C+Emanuela%3BGobitti%2C+Carlo%3BMinatel%2C+Emilio%3BPoliti%2C+Doriano%3BTalamini%2C+Renato%3BDi+Gennaro%2C+Giampiero%3BSavignano%2C+Gabriella%3BTrov%C3%B2%2C+Mauro+G%3BTirelli%2C+Umberto%3BBarzan%2C+Luigi&rft.aulast=Franchin&rft.aufirst=Giovanni&rft.date=2005-05-01&rft.volume=41&rft.issue=5&rft.spage=526&rft.isbn=&rft.btitle=&rft.title=Oral+oncology&rft.issn=13688375&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neuroprotection by the PGE2 EP2 receptor in permanent focal cerebral ischemia. AN - 67797641; 15852374 AB - Recent studies suggest a neuroprotective function of the PGE2 EP2 receptor in excitotoxic neuronal injury. The function of the EP2 receptor was examined at time points after excitotoxicity in an organotypic hippocampal model of N-methyl-D-aspartate (NMDA) challenge and in a permanent model of focal forebrain ischemia. Activation of EP2 led to significant neuroprotection in hippocampal slices up to 3 hours after a toxic NMDA stimulus. Genetic deletion of EP2 resulted in a marked increase in stroke volume in the permanent middle cerebral artery occlusion model. These findings support further investigation into therapeutic strategies targeting the EP2 receptor in stroke. JF - Annals of neurology AU - Liu, Dong AU - Wu, Liejun AU - Breyer, Richard AU - Mattson, Mark P AU - Andreasson, Katrin AD - Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, Baltimore, MD 21205, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 758 EP - 761 VL - 57 IS - 5 SN - 0364-5134, 0364-5134 KW - Excitatory Amino Acid Agonists KW - 0 KW - Ptger2 protein, mouse KW - Receptors, Prostaglandin E KW - Receptors, Prostaglandin E, EP2 Subtype KW - N-Methylaspartate KW - 6384-92-5 KW - Alprostadil KW - F5TD010360 KW - butaprost KW - HP16WVP23Y KW - Index Medicus KW - Animals KW - Cell Death -- physiology KW - Cerebral Infarction -- pathology KW - Mice KW - Mice, Knockout KW - Neurons -- pathology KW - Rats KW - Rats, Sprague-Dawley KW - N-Methylaspartate -- pharmacology KW - Mice, Inbred C57BL KW - Aging -- pathology KW - Excitatory Amino Acid Agonists -- pharmacology KW - Chronic Disease KW - Hippocampus -- pathology KW - Alprostadil -- pharmacology KW - Receptors, Prostaglandin E -- genetics KW - Brain Ischemia -- pathology KW - Alprostadil -- analogs & derivatives KW - Receptors, Prostaglandin E -- physiology KW - Receptors, Prostaglandin E -- agonists KW - Brain Ischemia -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67797641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+neurology&rft.atitle=Neuroprotection+by+the+PGE2+EP2+receptor+in+permanent+focal+cerebral+ischemia.&rft.au=Liu%2C+Dong%3BWu%2C+Liejun%3BBreyer%2C+Richard%3BMattson%2C+Mark+P%3BAndreasson%2C+Katrin&rft.aulast=Liu&rft.aufirst=Dong&rft.date=2005-05-01&rft.volume=57&rft.issue=5&rft.spage=758&rft.isbn=&rft.btitle=&rft.title=Annals+of+neurology&rft.issn=03645134&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-24 N1 - Date created - 2005-05-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Evidence for novel cannabinoid receptors. AN - 67786836; 15866316 AB - Cannabinoids, including the bioactive constituents of the marijuana plant, their synthetic analogs, and endogenous lipids with cannabinoid-like activity, produce their biological effects by interacting with specific receptors. To date, two G protein-coupled cannabinoid receptors have been identified by molecular cloning, CB1 receptors mainly expressed in the brain and mediating most of the neurobehavioral effects of cannabinoids and CB2 receptors expressed by immune and hematopoietic tissues. Recent findings indicate that some cannabinoid effects are not mediated by either CB1 or CB2 receptors, and in some cases there is compelling evidence to implicate additional receptors in these actions. These include transient receptor potential vanilloid 1 (TRPV1) receptors and as-yet-unidentified receptors implicated in the endothelium-dependent vasodilator effect of certain cannabinoids and in the presynaptic inhibition of glutamatergic neurotransmission in the hippocampus. The case for these additional receptors is being reviewed here. JF - Pharmacology & therapeutics AU - Begg, Malcolm AU - Pacher, Pál AU - Bátkai, Sándor AU - Osei-Hyiaman, Douglas AU - Offertáler, László AU - Mo, Fong Ming AU - Liu, Jie AU - Kunos, George AD - National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, MSC-9413 Bethesda, MD 8092-9413, United States. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 133 EP - 145 VL - 106 IS - 2 SN - 0163-7258, 0163-7258 KW - Cannabinoid Receptor Antagonists KW - 0 KW - Cannabinoids KW - Ion Channels KW - Piperidines KW - Pyrazoles KW - Receptors, Cannabinoid KW - TRPV Cation Channels KW - TRPV1 protein, human KW - rimonabant KW - RML78EN3XE KW - Index Medicus KW - Piperidines -- pharmacology KW - Pyrazoles -- pharmacology KW - Animals KW - Drug Interactions KW - Ion Channels -- pharmacology KW - Humans KW - Receptors, Cannabinoid -- physiology KW - Brain -- drug effects KW - Receptors, Cannabinoid -- drug effects KW - Brain -- metabolism KW - Cannabinoids -- pharmacology KW - Cannabinoids -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786836?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacology+%26+therapeutics&rft.atitle=Evidence+for+novel+cannabinoid+receptors.&rft.au=Begg%2C+Malcolm%3BPacher%2C+P%C3%A1l%3BB%C3%A1tkai%2C+S%C3%A1ndor%3BOsei-Hyiaman%2C+Douglas%3BOffert%C3%A1ler%2C+L%C3%A1szl%C3%B3%3BMo%2C+Fong+Ming%3BLiu%2C+Jie%3BKunos%2C+George&rft.aulast=Begg&rft.aufirst=Malcolm&rft.date=2005-05-01&rft.volume=106&rft.issue=2&rft.spage=133&rft.isbn=&rft.btitle=&rft.title=Pharmacology+%26+therapeutics&rft.issn=01637258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-30 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Identification of plasma membrane macro- and microdomains from wavelet analysis of FRET microscopy. AN - 67786764; 15722423 AB - In this study, we sought to characterize functional signaling domains by applying the multiresolution properties of the continuous wavelet transform to fluorescence resonance energy transfer (FRET) microscopic images of plasma membranes. A genetically encoded FRET reporter of protein kinase C (PKC)-dependent phosphorylation was expressed in COS1 cells. Differences between wavelet coefficient matrices revealed several heterogeneous domains (typically ranging from 1 to 5 microm), reflecting the dynamic balance between PKC and phosphatase activity during stimulation with phorbol-12,13-dibutyrate or acetylcholine. The balance in these domains was not necessarily reflected in the overall plasma membrane changes, and observed heterogeneity was absent when cells were exposed to a phosphatase or PKC inhibitor. Prolonged exposure to phorbol-12,13-dibutyrate and acetylcholine yielded more homogeneous FRET distribution in plasma membranes. The proposed wavelet-based image analysis provides, for the first time, a basis and a means of detecting and quantifying dynamic changes in functional signaling domains, and may find broader application in studying fine aspects of cellular signaling by various imaging reporters. JF - Biophysical journal AU - Kobrinsky, Evgeny AU - Mager, Donald E AU - Bentil, Sarah A AU - Murata, Shin-Ichi AU - Abernethy, Darrell R AU - Soldatov, Nikolai M AD - National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3625 EP - 3634 VL - 88 IS - 5 SN - 0006-3495, 0006-3495 KW - Adaptor Proteins, Signal Transducing KW - 0 KW - Bacterial Proteins KW - Cyan Fluorescent Protein KW - Luminescent Proteins KW - yellow fluorescent protein, Bacteria KW - Green Fluorescent Proteins KW - 147336-22-9 KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Phosphoric Monoester Hydrolases KW - EC 3.1.3.2 KW - Index Medicus KW - Animals KW - Green Fluorescent Proteins -- chemistry KW - COS Cells KW - Protein Kinase C -- genetics KW - Models, Statistical KW - Luminescent Proteins -- chemistry KW - Phosphoric Monoester Hydrolases -- metabolism KW - Adaptor Proteins, Signal Transducing -- chemistry KW - Phorbol 12,13-Dibutyrate -- chemistry KW - Bacterial Proteins -- chemistry KW - Phosphorylation KW - Protein Kinase C -- chemistry KW - Genes, Reporter KW - Protein Structure, Tertiary KW - Time Factors KW - Image Processing, Computer-Assisted KW - Signal Transduction KW - Cell Membrane -- metabolism KW - Fluorescence Resonance Energy Transfer -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786764?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biophysical+journal&rft.atitle=Identification+of+plasma+membrane+macro-+and+microdomains+from+wavelet+analysis+of+FRET+microscopy.&rft.au=Kobrinsky%2C+Evgeny%3BMager%2C+Donald+E%3BBentil%2C+Sarah+A%3BMurata%2C+Shin-Ichi%3BAbernethy%2C+Darrell+R%3BSoldatov%2C+Nikolai+M&rft.aulast=Kobrinsky&rft.aufirst=Evgeny&rft.date=2005-05-01&rft.volume=88&rft.issue=5&rft.spage=3625&rft.isbn=&rft.btitle=&rft.title=Biophysical+journal&rft.issn=00063495&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-18 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biochem. 2002 Nov;132(5):663-8 [12417013] Curr Opin Cell Biol. 2002 Aug;14(4):483-7 [12383800] J Cell Biol. 2003 Jun 9;161(5):899-909 [12782683] J Histochem Cytochem. 2003 Jul;51(7):951-8 [12810845] Biophys J. 2003 Jul;85(1):559-71 [12829510] Biophys J. 2003 Oct;85(4):2170-85 [14507683] Cell. 2004 Feb 20;116(4):577-89 [14980224] Nat Med. 2004 Mar;10(3):248-54 [14966518] Circ Res. 2004 Apr 16;94(7):866-73 [15087426] Biophys J. 2004 Aug;87(2):844-57 [15298893] J Cell Sci. 2004 Nov 1;117(Pt 23):5521-34 [15479718] Biophys J. 1993 Sep;65(3):1135-46 [8241393] J Microsc. 2004 Nov;216(Pt 2):110-22 [15516222] J Microsc. 2004 May;214(Pt 2):190-200 [15102066] Cytometry. 2001 Feb 1;43(2):94-100 [11169573] Methods. 2001 Jul;24(3):289-96 [11403577] Biophys J. 2001 Oct;81(4):2395-402 [11566809] Annu Rev Biomed Eng. 2000;2:511-50 [11701522] Science. 2002 May 3;296(5569):913-6 [11988576] J Biol Chem. 2003 Feb 14;278(7):5021-8 [12473653] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - How can drug addiction help us understand obesity? AN - 67786146; 15856062 JF - Nature neuroscience AU - Volkow, Nora D AU - Wise, Roy A AD - National Institute on Drug Abuse, National Institutes of Health, Bethesda, Maryland 20892, USA. nvolkow@nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 555 EP - 560 VL - 8 IS - 5 SN - 1097-6256, 1097-6256 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Neural Pathways -- physiopathology KW - Animals KW - Adaptation, Physiological -- physiology KW - Humans KW - Reinforcement (Psychology) KW - Neural Pathways -- physiology KW - Appetite Regulation -- physiology KW - Dopamine -- metabolism KW - Substance-Related Disorders -- physiopathology KW - Brain -- physiopathology KW - Behavior, Addictive -- psychology KW - Obesity -- psychology KW - Behavior, Addictive -- physiopathology KW - Substance-Related Disorders -- psychology KW - Obesity -- physiopathology KW - Brain -- growth & development UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786146?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Race+against+time.&rft.au=Fauci%2C+Anthony+S&rft.aulast=Fauci&rft.aufirst=Anthony&rft.date=2005-05-26&rft.volume=435&rft.issue=7041&rft.spage=423&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Efficacy, plasma pharmacokinetics, and safety of icofungipen, an inhibitor of Candida isoleucyl-tRNA synthetase, in treatment of experimental disseminated candidiasis in persistently neutropenic rabbits. AN - 67784556; 15855534 AB - Icofungipen (formerly PLD-118) is a synthetic derivative of the naturally occurring beta-amino acid cispentacin that blocks isoleucyl-tRNA synthetase, resulting in the inhibition of protein synthesis and growth of fungal cells. We investigated the efficacy, plasma pharmacokinetics, and safety of icofungipen in escalating dosages for the treatment of experimental subacute disseminated candidiasis in persistently neutropenic rabbits. Icofungipen was administered for 10 days starting 24 h after the intravenous inoculation of 10(3) Candida albicans blastoconidia. Study groups consisted of rabbits treated with icofungipen at 4 (ICO-4), 10 (ICO-10), and 25 (ICO-25) mg/kg of body weight/day in two divided dosages, rabbits treated with fluconazole at 10 mg/kg/day, rabbits treated with amphotericin B at 1 mg/kg/day, and untreated controls. Levels of icofungipen in plasma were derivatized by phthaldialdehyde and quantified by high-performance liquid chromatography with fluorescence detection. Rabbits treated with ICO-10 (P < 0.01) and ICO-25 (P < 0.001) showed significant dosage-dependent tissue clearance of C. albicans from the liver, spleen, kidney, brain, vitreous, vena cava, and lung in comparison to untreated controls. ICO-25 cleared C. albicans from all tissues and had activity comparable to that of amphotericin B versus untreated controls (P < 0.001). Pharmacokinetics of icofungipen in plasma approximated a dose-dependent relationship of the maximum concentration of drug in serum and the area under the concentration-time curve. There was no significant elevation of the levels of hepatic transaminases, alkaline phosphatase, bilirubin, urea nitrogen, or creatinine in icofungipen-treated rabbits. Icofungipen followed dose-dependent pharmacokinetics and was effective in the treatment of experimental disseminated candidiasis, including central nervous system infection, in persistently neutropenic rabbits. JF - Antimicrobial agents and chemotherapy AU - Petraitiene, Ruta AU - Petraitis, Vidmantas AU - Kelaher, Amy M AU - Sarafandi, Alia A AU - Mickiene, Diana AU - Groll, Andreas H AU - Sein, Tin AU - Bacher, John AU - Walsh, Thomas J AD - Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892-1100, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2084 EP - 2092 VL - 49 IS - 5 SN - 0066-4804, 0066-4804 KW - 2-amino-4-methylenecyclopentane-1-carboxylic acid KW - 0 KW - Antifungal Agents KW - Culture Media KW - Cycloleucine KW - 0TQU7668EI KW - Isoleucine-tRNA Ligase KW - EC 6.1.1.5 KW - Index Medicus KW - Animals KW - Spleen -- microbiology KW - Rabbits KW - Liver -- microbiology KW - Female KW - Kidney -- microbiology KW - Immunosuppression KW - Cycloleucine -- pharmacokinetics KW - Antifungal Agents -- adverse effects KW - Antifungal Agents -- pharmacokinetics KW - Cycloleucine -- pharmacology KW - Neutropenia -- complications KW - Candida albicans -- ultrastructure KW - Cycloleucine -- adverse effects KW - Cycloleucine -- analogs & derivatives KW - Candida albicans -- enzymology KW - Candidiasis -- microbiology KW - Candidiasis -- drug therapy KW - Antifungal Agents -- pharmacology KW - Isoleucine-tRNA Ligase -- antagonists & inhibitors KW - Candida albicans -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784556?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+agents+and+chemotherapy&rft.atitle=Efficacy%2C+plasma+pharmacokinetics%2C+and+safety+of+icofungipen%2C+an+inhibitor+of+Candida+isoleucyl-tRNA+synthetase%2C+in+treatment+of+experimental+disseminated+candidiasis+in+persistently+neutropenic+rabbits.&rft.au=Petraitiene%2C+Ruta%3BPetraitis%2C+Vidmantas%3BKelaher%2C+Amy+M%3BSarafandi%2C+Alia+A%3BMickiene%2C+Diana%3BGroll%2C+Andreas+H%3BSein%2C+Tin%3BBacher%2C+John%3BWalsh%2C+Thomas+J&rft.aulast=Petraitiene&rft.aufirst=Ruta&rft.date=2005-05-01&rft.volume=49&rft.issue=5&rft.spage=2084&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+agents+and+chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Trends Microbiol. 2000 May;8(5):200-1 [10785633] Clin Infect Dis. 1999 Aug;29(2):239-44 [10476719] Clin Infect Dis. 2001 Jun 15;32(12):1713-7 [11360213] Expert Opin Investig Drugs. 2001 Aug;10(8):1545-58 [11772269] Clin Infect Dis. 2002 Mar 1;34(5):600-2 [11810604] J Clin Microbiol. 2002 Apr;40(4):1298-302 [11923348] Antimicrob Agents Chemother. 2002 Jun;46(6):1857-69 [12019101] J Antimicrob Chemother. 2002 Jun;49(6):889-91 [12039879] J Clin Microbiol. 2002 Oct;40(10):3551-7 [12354845] N Engl J Med. 2002 Dec 19;347(25):2070-2 [12490691] Drug Discov Today. 2002 Mar 15;7(6):332-3 [11893534] Infect Dis Clin North Am. 2002 Dec;16(4):821-35 [12512183] Bioorg Med Chem Lett. 2003 Feb 10;13(3):433-6 [12565945] N Engl J Med. 2003 Apr 17;348(16):1546-54 [12700374] Expert Opin Pharmacother. 2003 May;4(5):807-23 [12740003] Clin Infect Dis. 2003 Sep 1;37(5):634-43 [12942393] Pediatrics. 2003 Sep;112(3 Pt 1):543-7 [12949281] Drug Resist Updat. 2003 Aug;6(4):197-218 [12962685] Lancet. 2003 Oct 4;362(9390):1142-51 [14550704] J Hosp Infect. 2003 Nov;55(3):159-68; quiz 233 [14572481] Clin Infect Dis. 2004 Apr 15;38(8):1119-27 [15095217] Pediatr Infect Dis J. 2004 Jul;23(7):635-41 [15247602] J Clin Microbiol. 1987 May;25(5):931-2 [3294892] Lab Anim Sci. 1988 Aug;38(4):467-71 [3184859] J Clin Microbiol. 1990 Jul;28(7):1616-22 [2380383] Antimicrob Agents Chemother. 1991 Jul;35(7):1321-8 [1929288] Can J Microbiol. 1991 Aug;37(8):637-46 [1954577] Antimicrob Agents Chemother. 1995 Jan;39(1):1-8 [7695288] Clin Infect Dis. 1995 Jun;20(6):1526-30 [7548503] Antimicrob Agents Chemother. 1997 Jun;41(6):1392-5 [9174207] Antimicrob Agents Chemother. 1998 May;42(5):1207-12 [9593151] Antimicrob Agents Chemother. 1998 Jul;42(7):1581-6 [9660987] Antimicrob Agents Chemother. 1998 Sep;42(9):2197-205 [9736535] Am J Health Syst Pharm. 1999 Mar 15;56(6):525-33; quiz 534-5 [10192687] Antimicrob Agents Chemother. 1999 Sep;43(9):2148-55 [10471556] Braz J Infect Dis. 2000 Aug;4(4):161-7 [11008219] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dietary supplements in weight reduction. AN - 67784457; 15867902 AB - We summarize evidence on the role of dietary supplements in weight reduction, with particular attention to their safety and benefits. Dietary supplements are used for two purposes in weight reduction: (a) providing nutrients that may be inadequate in calorie-restricted diets and (b) for their potential benefits in stimulating weight loss. The goal in planning weight-reduction diets is that total intake from food and supplements should meet recommended dietary allowance/adequate intake levels without greatly exceeding them for all nutrients, except energy. If nutrient amounts from food sources in the reducing diet fall short, dietary supplements containing a single nutrient/element or a multivitamin-mineral combination may be helpful. On hypocaloric diets, the addition of dietary supplements providing nutrients at a level equal to or below recommended dietary allowance/adequate intake levels or 100% daily value, as stated in a supplement's facts box on the label, may help dieters to achieve nutrient adequacy and maintain electrolyte balance while avoiding the risk of excessive nutrient intakes. Many botanical and other types of dietary supplements are purported to be useful for stimulating or enhancing weight loss. Evidence of their efficacy in stimulating weight loss is inconclusive at present. Although there are few examples of safety concerns related to products that are legal and on the market for this purpose, there is also a paucity of evidence on safety for this intended use. Ephedra and ephedrine-containing supplements, with or without caffeine, have been singled out in recent alerts from the Food and Drug Administration because of safety concerns, and use of products containing these substances cannot be recommended. Dietitians should periodically check the Food and Drug Administration Web site ( www.cfsan.fda.gov ) for updates and warnings and alert patients/clients to safety concerns. Dietetics professionals should also consult authoritative sources for new data on efficacy as it becomes available ( ods.od.nih.gov ). JF - Journal of the American Dietetic Association AU - Dwyer, Johanna T AU - Allison, David B AU - Coates, Paul M AD - Office of Dietary Supplements, National Institutes of Health, Bethesda, Maryland 20892, USA. DwyerJ1@od.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - S80 EP - S86 VL - 105 IS - 5 Suppl 1 SN - 0002-8223, 0002-8223 KW - Anti-Obesity Agents KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Nutritional Requirements KW - United States Food and Drug Administration KW - Consumer Product Safety KW - Humans KW - Safety KW - Nutrition Policy KW - Treatment Outcome KW - Caloric Restriction KW - Obesity -- drug therapy KW - Weight Loss -- drug effects KW - Dietary Supplements KW - Anti-Obesity Agents -- adverse effects KW - Anti-Obesity Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=World+journal+of+gastroenterology&rft.atitle=Mismatch+repair+genes+%28hMLH1%2C+hPMS1%2C+hPMS2%2C+GTBP%2FhMSH6%2C+hMSH2%29+in+the+pathogenesis+of+hepatocellular+carcinoma.&rft.au=Zekri%2C+Abdel-Rahman+N%3BSabry%2C+Gelane+M%3BBahnassy%2C+Abeer+A%3BShalaby%2C+Kamal+A%3BAbdel-Wahabh%2C+Sabrin+A%3BZakaria%2C+Serag&rft.aulast=Zekri&rft.aufirst=Abdel-Rahman&rft.date=2005-05-28&rft.volume=11&rft.issue=20&rft.spage=3020&rft.isbn=&rft.btitle=&rft.title=World+journal+of+gastroenterology&rft.issn=10079327&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Combining a recombinant cancer vaccine with standard definitive radiotherapy in patients with localized prostate cancer. AN - 67784255; 15867235 AB - Many patients with clinically localized prostate cancer develop biochemical failure despite excellent local therapy perhaps due to occult metastatic disease. One potential solution is the utilization of a well-tolerated systemic therapy (e.g., vaccine) in concert with local therapy. We present a randomized phase II clinical trial designed to determine if a poxviral vaccine encoding prostate-specific antigen (PSA) can induce a PSA-specific T-cell response when combined with radiotherapy in patients with clinically localized prostate cancer. Thirty patients were randomized in a 2:1 ratio into vaccine plus radiotherapy or radiotherapy-only arms. Those patients in the combination arm received a "priming" vaccine with recombinant vaccinia (rV) PSA plus r V containing the T-cell costimulatory molecule B7.1 (rV-B7.1) followed by monthly booster vaccines with recombinant fowlpox PSA. The vaccines were given with local granulocyte-macrophage colony-stimulating factor and low-dose systemic interleukin-2. Standard external beam radiation therapy was given between the fourth and the sixth vaccinations. Seventeen of 19 patients in the combination arm completed all eight vaccinations and 13 of these 17 patients had increases in PSA-specific T cells of at least 3-fold versus no detectable increases in the radiotherapy-only arm (P < 0.0005). There was also evidence of de novo generation of T cells to well-described prostate-associated antigens not found in the vaccine, providing indirect evidence of immune-mediated tumor killing. The vaccine was well tolerated. This vaccine regimen can be safely given in patients undergoing radiation therapy for localized prostate cancer, with the majority of patients generating a PSA-specific cellular immune response to vaccine. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Gulley, James L AU - Arlen, Philip M AU - Bastian, Anne AU - Morin, Steven AU - Marte, Jennifer AU - Beetham, Patricia AU - Tsang, Kwong-Yok AU - Yokokawa, Junko AU - Hodge, James W AU - Ménard, Cynthia AU - Camphausen, Kevin AU - Coleman, C Norman AU - Sullivan, Francis AU - Steinberg, Seth M AU - Schlom, Jeffrey AU - Dahut, William AD - Laboratory of Tumor Immunology and Biology, Medical Oncology Clinical Research Unit, Radiation Oncology Branch, and Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 3353 EP - 3362 VL - 11 IS - 9 SN - 1078-0432, 1078-0432 KW - Antigens, CD80 KW - 0 KW - Cancer Vaccines KW - Recombinant Fusion Proteins KW - Prostate-Specific Antigen KW - EC 3.4.21.77 KW - Index Medicus KW - Recombinant Fusion Proteins -- immunology KW - Combined Modality Therapy KW - Humans KW - Antigens, CD80 -- genetics KW - Aged KW - Cell Line, Tumor KW - Cytotoxicity, Immunologic -- immunology KW - Antigens, CD80 -- immunology KW - Tumor Cells, Cultured KW - Aged, 80 and over KW - Prostate-Specific Antigen -- blood KW - Treatment Outcome KW - Cytotoxicity Tests, Immunologic KW - Middle Aged KW - Recombinant Fusion Proteins -- therapeutic use KW - Cell Line KW - Male KW - Prostatic Neoplasms -- immunology KW - Cancer Vaccines -- immunology KW - Cancer Vaccines -- adverse effects KW - Cancer Vaccines -- therapeutic use KW - Prostatic Neoplasms -- therapy KW - Prostatic Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Combining+a+recombinant+cancer+vaccine+with+standard+definitive+radiotherapy+in+patients+with+localized+prostate+cancer.&rft.au=Gulley%2C+James+L%3BArlen%2C+Philip+M%3BBastian%2C+Anne%3BMorin%2C+Steven%3BMarte%2C+Jennifer%3BBeetham%2C+Patricia%3BTsang%2C+Kwong-Yok%3BYokokawa%2C+Junko%3BHodge%2C+James+W%3BM%C3%A9nard%2C+Cynthia%3BCamphausen%2C+Kevin%3BColeman%2C+C+Norman%3BSullivan%2C+Francis%3BSteinberg%2C+Seth+M%3BSchlom%2C+Jeffrey%3BDahut%2C+William&rft.aulast=Gulley&rft.aufirst=James&rft.date=2005-05-01&rft.volume=11&rft.issue=9&rft.spage=3353&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):6757-62 [16203760] Erratum In: Clin Cancer Res. 2006 Jan 1;12(1):322 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Adjusting lung cancer risks for temporal and spatial variations in radon concentration in dwellings in Gansu Province, China. AN - 67783493; 15850419 AB - Our recent study in Gansu Province, China reported an increasing risk of lung cancer with increasing residential radon concentration that was consistent with previous pooled analyses and with meta-analyses of other residential studies (Wang et al., Am. J. Epidemiol. 155, 554-564, 2002). Dosimetry used current radon measurements (1-year track-etch detectors) in homes to characterize concentrations for the previous 30 years, resulting in uncertainties in exposure and possibly reduced estimates of disease risk. We conducted a 3-year substudy in 55 houses to model the temporal and spatial variability in radon levels and to adjust estimates of radon risk. Temporal variation represented the single largest source of uncertainty, suggesting the usefulness of multi-year measurements to assess this variation; however, substantial residual variation remained unexplained. The uncertainty adjustment increased estimates of the excess odds ratio by 50-100%, suggesting that residential radon studies using similar dosimetry may also underestimate radon effects. These results have important implications for risk assessment. JF - Radiation research AU - Lubin, J H AU - Wang, Z Y AU - Wang, L D AU - Boice, J D AU - Cui, H X AU - Zhang, S R AU - Conrath, S AU - Xia, Y AU - Shang, B AU - Cao, J S AU - Kleinerman, R A AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA. lubinj@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 571 EP - 579 VL - 163 IS - 5 SN - 0033-7587, 0033-7587 KW - Radon KW - Q74S4N8N1G KW - Index Medicus KW - Space life sciences KW - Regression Analysis KW - Housing KW - Humans KW - Adult KW - Case-Control Studies KW - Aged KW - Middle Aged KW - Likelihood Functions KW - Risk Assessment KW - Air Pollution, Indoor -- adverse effects KW - Lung Neoplasms -- etiology KW - Neoplasms, Radiation-Induced -- etiology KW - Radon -- analysis KW - Radon -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783493?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+research&rft.atitle=Adjusting+lung+cancer+risks+for+temporal+and+spatial+variations+in+radon+concentration+in+dwellings+in+Gansu+Province%2C+China.&rft.au=Lubin%2C+J+H%3BWang%2C+Z+Y%3BWang%2C+L+D%3BBoice%2C+J+D%3BCui%2C+H+X%3BZhang%2C+S+R%3BConrath%2C+S%3BXia%2C+Y%3BShang%2C+B%3BCao%2C+J+S%3BKleinerman%2C+R+A&rft.aulast=Lubin&rft.aufirst=J&rft.date=2005-05-01&rft.volume=163&rft.issue=5&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Radiation+research&rft.issn=00337587&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-02 N1 - Date created - 2005-04-26 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Radiat Res. 2006 Jul;166(1 Pt 1):120; author reply 121 [16808598] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Supra-additive growth inhibition by a celecoxib analogue and carboxyamido-triazole is primarily mediated through apoptosis. AN - 67783362; 15867384 AB - Combination studies of celecoxib and chemotherapeutic agents suggest that combining cyclooxygenase-2 inhibitors with other agents may have supra-additive or synergistic effects on tumor growth inhibition. Carboxyamido-triazole (CAI), a voltage-independent calcium channel inhibitor, has been shown to induce growth inhibition and apoptosis in cancer cells. We found that continuous exposure to cytostatic doses of CAI and LM-1685, a celecoxib analogue, reduced the proliferation and survival of seven human cancer cell lines by at least one log (P < or = 0.001) over either agent alone. To explore the mechanism of action of this combination, we further studied the effects of LM-1685/CAI on CCL-250 colorectal carcinoma cells. We found that the supra-additive antiproliferative effects occurred throughout a range of LM-1685 doses (5-25 micromol/L) and paralleled a decrease in COX-2 activity as measured by prostaglandin E2 production. In these cells, treatment with LM-1685/CAI suppressed the extracellular signal-regulated kinase pathway within the first hour but ultimately results in high, sustained activation of ERK over a 9-day period (P = 0.0005). Suppression of cyclin D1 and phospho-AKT, and cleavage of caspase-3 and PARP were concomitant with persistent ERK activation. Addition of PD98059, a MEK-1 inhibitor, suppressed ERK activation and significantly but incompletely reversed these signaling events and apoptosis. Flow cytometry experiments revealed that the CAI/LM-1685 combination induced a 3-fold increase in apoptosis over control (P = 0.005) in 3 days. We show that the combination of CAI and LM-1685 produces a cytotoxic effect by suppressing proliferation and triggering apoptosis. JF - Cancer research AU - Winters, Mary E AU - Mehta, Arpita I AU - Petricoin, Emanuel F AU - Kohn, Elise C AU - Liotta, Lance A AD - Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 3853 EP - 3860 VL - 65 IS - 9 SN - 0008-5472, 0008-5472 KW - Calcium Channel Blockers KW - 0 KW - Cyclooxygenase 2 Inhibitors KW - Cyclooxygenase Inhibitors KW - Indoles KW - LM-1685 KW - Membrane Proteins KW - Pyrazoles KW - Sulfonamides KW - Triazoles KW - carboxyamido-triazole KW - 99519-84-3 KW - Cyclooxygenase 2 KW - EC 1.14.99.1 KW - PTGS2 protein, human KW - Prostaglandin-Endoperoxide Synthases KW - Receptor, Epidermal Growth Factor KW - EC 2.7.10.1 KW - Celecoxib KW - JCX84Q7J1L KW - Dinoprostone KW - K7Q1JQR04M KW - Index Medicus KW - Pyrazoles -- administration & dosage KW - Drug Screening Assays, Antitumor KW - Dinoprostone -- pharmacology KW - Humans KW - Transcriptional Activation -- drug effects KW - Cell Line, Tumor KW - Sulfonamides -- administration & dosage KW - Prostaglandin-Endoperoxide Synthases -- metabolism KW - Signal Transduction -- drug effects KW - Drug Synergism KW - Cell Cycle -- drug effects KW - Calcium Channel Blockers -- pharmacology KW - Cyclooxygenase Inhibitors -- administration & dosage KW - Apoptosis -- drug effects KW - Calcium Channel Blockers -- administration & dosage KW - Indoles -- pharmacology KW - Antineoplastic Combined Chemotherapy Protocols -- pharmacology KW - Triazoles -- pharmacology KW - Triazoles -- administration & dosage KW - Cyclooxygenase Inhibitors -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783362?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Supra-additive+growth+inhibition+by+a+celecoxib+analogue+and+carboxyamido-triazole+is+primarily+mediated+through+apoptosis.&rft.au=Winters%2C+Mary+E%3BMehta%2C+Arpita+I%3BPetricoin%2C+Emanuel+F%3BKohn%2C+Elise+C%3BLiotta%2C+Lance+A&rft.aulast=Winters&rft.aufirst=Mary&rft.date=2005-05-01&rft.volume=65&rft.issue=9&rft.spage=3853&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-15 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Overview of aerosolized Florida red tide toxins: exposures and effects. AN - 67783255; 15866773 AB - Florida red tide is caused by Karenia brevis, a dinoflagellate that periodically blooms, releasing its potent neurotoxin, brevetoxin, into the surrounding waters and air along the coast of the Gulf of Mexico. Exposure to Florida red tide toxins has been associated with adverse human health effects and massive fish and marine mammal deaths. The articles in this mini-monograph describe the ongoing interdisciplinary and interagency research program that characterizes the exposures and health effects of aerosolized Florida red tide toxins (brevetoxins). The interdisciplinary research program uses animal models and laboratory studies to develop hypotheses and apply these findings to in situ human exposures. Our ultimate goal is to develop appropriate prevention measures and medical interventions to mitigate or prevent adverse health effects from exposure to complex mixtures of aerosolized red tide toxins. JF - Environmental health perspectives AU - Fleming, Lora E AU - Backer, Lorraine C AU - Baden, Daniel G AD - National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Sciences Center, University of Miami Rosenstiel School of Marine and Atmospheric Sciences, Miami, FL 33136, USA. lfleming@med.miami.edu Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 618 EP - 620 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Aerosols KW - 0 KW - Marine Toxins KW - Oxocins KW - brevetoxin KW - 98225-48-0 KW - Index Medicus KW - Environmental Monitoring KW - Animals KW - Eutrophication KW - Humans KW - Research -- trends KW - Florida KW - Public Health KW - Oxocins -- adverse effects KW - Dinoflagellida -- pathogenicity KW - Marine Toxins -- adverse effects KW - Environmental Exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Overview+of+aerosolized+Florida+red+tide+toxins%3A+exposures+and+effects.&rft.au=Fleming%2C+Lora+E%3BBacker%2C+Lorraine+C%3BBaden%2C+Daniel+G&rft.aulast=Fleming&rft.aufirst=Lora&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=618&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 2004 Jun;112(8):A455-6 [15175187] Environ Health Perspect. 2004 Jun;112(8):A454-5 [15175186] Environ Lett. 1972;3(4):271-8 [4627989] JFMA. 1973 Nov;60(11):27-9 [4755462] Int Rev Cytol. 1983;82:99-150 [6352551] Mol Pharmacol. 1991 Dec;40(6):988-94 [1661842] Nat Toxins. 1999;7(2):45-8 [10495465] Environ Health Perspect. 2005 May;113(5):621-5 [15866774] Nat Toxins. 1994;2(4):212-21 [7952946] Indian J Exp Biol. 1997 Jun;35(6):650-4 [9357171] Toxicol Pathol. 1998 Mar-Apr;26(2):276-82 [9547868] J Toxicol Environ Health A. 1999 Jul 9;57(5):345-55 [10405188] Environ Health Perspect. 2005 May;113(5):626-31 [15866775] Environ Health Perspect. 2005 May;113(5):632-7 [15866776] Environ Health Perspect. 2005 May;113(5):638-43 [15866777] Environ Health Perspect. 2005 May;113(5):644-9 [15866778] Environ Health Perspect. 2005 May;113(5):650-7 [15866779] J Soc Biol. 1999;193(3):329-44 [10542966] Neurotoxicology. 1999 Dec;20(6):909-20 [10693972] Environ Health Perspect. 2000 Mar;108 Suppl 1:133-41 [10698729] Toxicon. 2000 Jul;38(7):981-93 [10728835] Chem Biol. 2000 Jun;7(6):385-93 [10873835] Environ Toxicol Chem. 2001 Jan;20(1):107-14 [11351396] Environ Health Perspect. 2002 Feb;110(2):179-85 [11836147] Environ Health Perspect. 2002 Sep;110(9):839-45 [12204815] Cell Mol Neurobiol. 2004 Aug;24(4):553-63 [15233378] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Scientific vision: setting forth a strategy. AN - 67783162; 15866748 JF - Environmental health perspectives AU - Schwartz, David A AD - National Institute of Environmental Health Sciences/NIH, Durham, NC, USA. schwartzd@niehs.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Environmental Pollutants KW - 0 KW - Index Medicus KW - United States KW - Humans KW - Environmental Pollutants -- poisoning KW - Risk Assessment KW - Research Support as Topic KW - Environmental Health KW - National Institutes of Health (U.S.) -- organization & administration KW - Environmental Exposure KW - Organizational Objectives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783162?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Scientific+vision%3A+setting+forth+a+strategy.&rft.au=Schwartz%2C+David+A&rft.aulast=Schwartz&rft.aufirst=David&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=A292&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serpiginous choroiditis. AN - 67778535; 15850812 AB - Serpiginous choroiditis is a rare, usually bilateral, chronic, progressive, recurrent inflammation of the choroid, retinal pigment epithelium, and choriocapillaris of unknown etiology. Based on clinical presentation, it can be classified into 1) peripapillary, 2) macular, and 3) ampiginous types. The clinical course, regardless of the presentation, is progressive with multiple recurrences leading to potentially significant visual loss. Visual outcome is directly related to the involvement of the para-fovea and fovea by the lesions or secondary choroidal neovascularization. The histological findings of the lesions are atrophy of the choriocapillaris, retinal pigment epithelium and photoreceptor cells, and moderate diffuse lymphocytic infiltrates throughout the choroid. Multiple etiologies including autoimmunity, infection, vasculopathy, and degeneration were proposed but none is well supported by clinical and laboratory evidence. Fluorescein and indocyanine green angiography have been useful in the assessment of the extent and the activity of lesions. Due to the insidious and progressive clinical course, an assessment of treatment outcomes needs long term follow-up. Currently, treatment with immunosuppressive and alkylating agents have shown possible efficacy in small case series. Larger clinical studies and interventional trials are required to further our understanding of the pathogenesis, etiology, and for the evaluation of treatment strategies. JF - Survey of ophthalmology AU - Lim, Wee-Kiak AU - Buggage, Ronald R AU - Nussenblatt, Robert B AD - National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA; and Singapore National Eye Centre, Singapore. PY - 2005 SP - 231 EP - 244 VL - 50 IS - 3 SN - 0039-6257, 0039-6257 KW - Antimetabolites, Antineoplastic KW - 0 KW - Coloring Agents KW - Immunosuppressive Agents KW - Indocyanine Green KW - IX6J1063HV KW - Index Medicus KW - Photoreceptor Cells, Vertebrate -- pathology KW - Diagnosis, Differential KW - Humans KW - Fluorescein Angiography KW - Atrophy KW - Pigment Epithelium of Eye -- pathology KW - Immunosuppressive Agents -- therapeutic use KW - Antimetabolites, Antineoplastic -- therapeutic use KW - Choroiditis -- diagnosis KW - Choroiditis -- epidemiology KW - Choroiditis -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67778535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Survey+of+ophthalmology&rft.atitle=Serpiginous+choroiditis.&rft.au=Lim%2C+Wee-Kiak%3BBuggage%2C+Ronald+R%3BNussenblatt%2C+Robert+B&rft.aulast=Lim&rft.aufirst=Wee-Kiak&rft.date=2005-05-01&rft.volume=50&rft.issue=3&rft.spage=231&rft.isbn=&rft.btitle=&rft.title=Survey+of+ophthalmology&rft.issn=00396257&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A Phase I clinical trial of ixabepilone (BMS-247550), an epothilone B analog, administered intravenously on a daily schedule for 3 days. AN - 67770263; 15800893 AB - The epothilones are a novel class of microtubule-stabilizing agents. Ixabepilone (BMS-247550; NSC 710428) is a semisynthetic analog of the natural product epothilone B. The authors conducted a Phase I study by administering ixabepilone to patients as a 1-hour intravenous infusion daily for 3 consecutive days every 21 days. Twenty-six patients were enrolled and received ixabepilone at a starting dose of 8 or 10 mg/m(2) per day for 3 consecutive days. One hundred and nineteen cycles were administered to 26 patients. The maximum-tolerated dose was 8 mg/m(2) per day of ixabepilone administered as a 1-hour intravenous infusion daily for 3 consecutive days every 21 days. The dose-limiting toxicity (DLT) was neutropenia. Other nonhematologic Grade 3 toxicities included fatigue (3 cycles), hyponatremia (1 cycle), anorexia (1 cycle), ileus (1 cycle), stomatitis (1 cycle), and emesis (1 cycle). Prolonged disease stabilization was observed in patients with mesothelioma, ovarian carcinoma, and renal cell carcinoma. The recommended Phase II dose of ixabepilone on the daily schedule for 3 days was 8-10 mg/m(2) per day. Neutropenia was the DLT. Peripheral neuropathy was mild, even after multiple cycles of therapy, and was not dose limiting. Published 2005 by the American Cancer Society. JF - Cancer AU - Zhuang, Sen H AU - Agrawal, Manish AU - Edgerly, Maureen AU - Bakke, Susan AU - Kotz, Herb AU - Thambi, Paul AU - Rutt, Ann AU - Balis, Frank M AU - Bates, Susan AU - Fojo, Tito AD - Cancer Therapeutics Branch, Center of Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 1932 EP - 1938 VL - 103 IS - 9 SN - 0008-543X, 0008-543X KW - Epothilones KW - 0 KW - ixabepilone KW - K27005NP0A KW - Abridged Index Medicus KW - Index Medicus KW - Drug Administration Schedule KW - Infusions, Intravenous KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Maximum Tolerated Dose KW - Male KW - Female KW - Neoplasms -- drug therapy KW - Epothilones -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67770263?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer&rft.atitle=A+Phase+I+clinical+trial+of+ixabepilone+%28BMS-247550%29%2C+an+epothilone+B+analog%2C+administered+intravenously+on+a+daily+schedule+for+3+days.&rft.au=Zhuang%2C+Sen+H%3BAgrawal%2C+Manish%3BEdgerly%2C+Maureen%3BBakke%2C+Susan%3BKotz%2C+Herb%3BThambi%2C+Paul%3BRutt%2C+Ann%3BBalis%2C+Frank+M%3BBates%2C+Susan%3BFojo%2C+Tito&rft.aulast=Zhuang&rft.aufirst=Sen&rft.date=2005-05-01&rft.volume=103&rft.issue=9&rft.spage=1932&rft.isbn=&rft.btitle=&rft.title=Cancer&rft.issn=0008543X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An essential role for phospholipase D in the activation of protein kinase C and degranulation in mast cells. AN - 67768072; 15843515 AB - Activation of phospholipase D (PLD) and protein kinase C (PKC) as well as calcium mobilization are essential signals for degranulation of mast cells. However, the exact role of PLD in degranulation remains undefined. In this study we have tested the hypothesis that the PLD product, phosphatidic acid, and diacylglycerides generated therefrom might promote activation of PKC. Studies were conducted in two rodent mast cell lines that were stimulated with Ag via FcepsilonRI and a pharmacologic agent, thapsigargin. Diversion of production of phosphatidic acid to phosphatidylbutanol (the transphosphatidylation reaction) by addition of l-butanol suppressed both the translocation of diacylglyceride-dependent isoforms of PKC to the membrane and degranulation. Tertiary-butanol, which is not a substrate for the transphosphatidylation, had a minimal effect on PKC translocation and degranulation, and 1-butanol itself had no effect on PKC translocation when PKC was stimulated directly with phorbol ester, 12-O-tetradecanoylphorbol-13-acetate. Also, in cells transfected with small inhibitory RNAs directed against PLD1 and PLD2, activation of PLD, generation of diacylglycerides, translocation of PKC, and degranulation were all suppressed. Phorbol ester, which did not stimulate degranulation by itself, restored degranulation when used in combination with thapsigargin whether PLD function was disrupted with 1-butanol or the small inhibitory RNAs. However, degranulation was not restored when cells were costimulated with Ag and phorbol ester. These results suggested that the production of phosphatidic acid by PLD facilitates activation of PKC and, in turn, degranulation, although additional PLD-dependent processes appear to be critical for Ag-mediated degranulation. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Peng, Ze AU - Beaven, Michael A AD - Laboratory of Molecular Immunology, National, Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5201 EP - 5208 VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - Diglycerides KW - 0 KW - Enzyme Inhibitors KW - Isoenzymes KW - Phosphatidic Acids KW - RNA, Small Interfering KW - Thapsigargin KW - 67526-95-8 KW - 1-Butanol KW - 8PJ61P6TS3 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Phospholipase D KW - EC 3.1.4.4 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Abridged Index Medicus KW - Index Medicus KW - 1-Butanol -- pharmacology KW - Phosphatidic Acids -- biosynthesis KW - Animals KW - Phosphatidic Acids -- antagonists & inhibitors KW - Protein Transport -- drug effects KW - Diglycerides -- biosynthesis KW - Mice KW - 1-Butanol -- antagonists & inhibitors KW - Cell Line, Tumor KW - Isoenzymes -- metabolism KW - Thapsigargin -- pharmacology KW - Rats KW - Isoenzymes -- antagonists & inhibitors KW - Phosphorylation KW - Mice, Inbred C57BL KW - Enzyme Activation -- drug effects KW - Tetradecanoylphorbol Acetate -- pharmacology KW - RNA, Small Interfering -- pharmacology KW - Enzyme Inhibitors -- pharmacology KW - Cell Line, Transformed KW - Drug Synergism KW - Enzyme Activation -- immunology KW - Diglycerides -- antagonists & inhibitors KW - Protein Transport -- immunology KW - Protein Kinase C -- metabolism KW - Protein Kinase C -- antagonists & inhibitors KW - Mast Cells -- metabolism KW - Phospholipase D -- antagonists & inhibitors KW - Mast Cells -- enzymology KW - Phospholipase D -- physiology KW - Mast Cells -- drug effects KW - Cell Degranulation -- immunology KW - Cell Degranulation -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67768072?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=An+essential+role+for+phospholipase+D+in+the+activation+of+protein+kinase+C+and+degranulation+in+mast+cells.&rft.au=Peng%2C+Ze%3BBeaven%2C+Michael+A&rft.aulast=Peng&rft.aufirst=Ze&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5201&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparison of asbestos exposure assessments by next-of-kin respondents, by an occupational hygienist, and by a job-exposure matrix from the National Occupational Hazard Survey. AN - 67759019; 15828074 AB - Assessments of occupational exposures in case-control studies of rapidly fatal illnesses often rely on data from next-of-kin respondents, which may be inaccurate. Three methods for assessing exposure to asbestos from case-control data on mesothelioma, including next-of-kin assessment, expert assessment, and use of a generic job-exposure matrix (JEM). Interview data [Spirtas et al. (1994): Occup Environ Med 51:804-811] were reviewed to determine exposure status by an occupational hygienist (C.R.) who was unaware of disease status. Exposure odds ratios were calculated using standard methods, and measures of agreement included the kappa statistic and conditional and marginal odds ratios. Expert assessment detected higher proportions of exposed subjects than the next-of-kin respondents or JEM methods. The disease-exposure odds ratios were highest for respondents, perhaps because of recall bias, and lowest for the JEM method. The agreement was highest between the respondent and expert assessments. A combination of respondent's assessment and JEM assessment led to the best prediction of the expert's assessment. Results for spouse respondents were similar to those for other "next-of-kin" respondents. Expert assessments were the most plausible, but the data indicate that disease associations could also be detected with the other exposure assessment methods. Using some combination of the proxy respondent's assessment and the JEM assessment, one can predict the expert's assessment. A strategy that relied on the respondent's assessment when it was positive and otherwise obtained an expert assessment could reduce costs with little error, compared to expert assessment on all subjects. Published 2005 Wiley-Liss, Inc. JF - American journal of industrial medicine AU - Nam, Jun-mo AU - Rice, Carol AU - Gail, Mitchell H AD - Biostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health & Human Services, Bethesda, Maryland, USA. namj@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 443 EP - 450 VL - 47 IS - 5 SN - 0271-3586, 0271-3586 KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Los Angeles -- epidemiology KW - Reproducibility of Results KW - Humans KW - Aged KW - Mental Recall KW - Risk Assessment -- methods KW - New York -- epidemiology KW - Registries KW - Proxy KW - Aged, 80 and over KW - Adult KW - Death Certificates KW - Health Surveys KW - Case-Control Studies KW - Middle Aged KW - Bias (Epidemiology) KW - Occupational Medicine KW - Occupational Exposure -- statistics & numerical data KW - Mesothelioma -- epidemiology KW - Mesothelioma -- etiology KW - Mesothelioma -- mortality KW - Occupational Exposure -- adverse effects KW - Family KW - Asbestos -- toxicity KW - Occupational Exposure -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67759019?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3A&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology&rft.atitle=Neural+basis+of+mindfulness+interventions+that+moderate+the+impact+of+stress+on+the+brain&rft.au=Paulus%2C+Martin+P.&rft.aulast=Paulus&rft.aufirst=Martin&rft.date=2016-01-01&rft.volume=41&rft.issue=1&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/10.1038%2Fnpp.2015.239 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Occupation and risk of stomach cancer in Poland. AN - 67756077; 15837853 AB - In spite of the dramatic decline in the incidence of stomach cancer in the twentieth century, Poland has one of the highest rates in the world. To evaluate the risk of stomach cancer by grouped occupations and industries, as well as by some specific occupational exposures. Cases (n = 443) were newly diagnosed with stomach adenocarcinomas between 1994 and 1996. Controls (n = 479) were randomly selected from the general population in Warsaw. Only a few occupations and industries were associated with significantly increased risks of stomach cancer. The most suggestive finding was for work in the leather goods industry. Risk was also significantly increased among men working in fabricated metal production and among women ever employed as managers and governmental officials. Men ever employed as teaching professionals and women employed as technical and science professionals had significantly decreased risks of stomach cancer. Among men, a significant positive trend in risk with duration of employment was observed for work in the leather industry and special trade construction. No significantly increased risks were observed for specific exposures assessed by a job-exposure matrix or by self-reports. However among men there were non-significantly increased risks with 10 or more years exposure to asbestos, metal dust, and nitrosamines assessed by a job-exposure matrix. Employment in the leather goods industry, special trade construction, and metal fabrication was associated with an increased risk of stomach cancer among men. However, there were only weak associations with specific exposures. Occupational exposures do not contribute substantially to the high rates of stomach cancer in Poland. JF - Occupational and environmental medicine AU - Krstev, S AU - Dosemeci, M AU - Lissowska, J AU - Chow, W-H AU - Zatonski, W AU - Ward, M H AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd, EPS 8104, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 318 EP - 324 VL - 62 IS - 5 KW - Carcinogens, Environmental KW - 0 KW - Dust KW - Nitrosamines KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Nitrosamines -- toxicity KW - Odds Ratio KW - Poland -- epidemiology KW - Humans KW - Asbestos -- toxicity KW - Carcinogens, Environmental -- toxicity KW - Adenocarcinoma -- epidemiology KW - Risk Factors KW - Case-Control Studies KW - Occupational Exposure -- adverse effects KW - Administrative Personnel KW - Middle Aged KW - Time Factors KW - Female KW - Male KW - Metallurgy KW - Industry KW - Occupational Diseases -- epidemiology KW - Stomach Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67756077?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Occupational+and+environmental+medicine&rft.atitle=Occupation+and+risk+of+stomach+cancer+in+Poland.&rft.au=Krstev%2C+S%3BDosemeci%2C+M%3BLissowska%2C+J%3BChow%2C+W-H%3BZatonski%2C+W%3BWard%2C+M+H&rft.aulast=Krstev&rft.aufirst=S&rft.date=2005-05-01&rft.volume=62&rft.issue=5&rft.spage=318&rft.isbn=&rft.btitle=&rft.title=Occupational+and+environmental+medicine&rft.issn=1470-7926&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer Res. 1999 Dec 1;59(23):5932-7 [10606238] Int J Cancer. 1999 Sep 24;83(1):18-29 [10449602] Occup Environ Med. 2002 May;59(5):329-37 [11983848] Am J Ind Med. 2002 Jul;42(1):11-22 [12111686] Curr Gastroenterol Rep. 2002 Dec;4(6):463-70 [12441036] Postgrad Med J. 2003 May;79(931):252-8 [12782770] Nutr Cancer. 2004;48(2):149-59 [15231449] Am Rev Respir Dis. 1980 May;121(5):887-92 [7406323] Am J Ind Med. 1990;18(1):69-78 [2378371] Br J Ind Med. 1990 Sep;47(9):602-10 [2207031] Scand J Work Environ Health. 1991 Aug;17(4):240-7 [1925435] Cancer Causes Control. 1994 May;5(3):241-8 [8061172] Am J Ind Med. 1994 Oct;26(4):511-20 [7810549] Cancer Surv. 1994;19-20:55-76 [7534641] Cancer Lett. 1995 Jun 29;93(1):17-48 [7600541] Am J Ind Med. 1996 Jul;30(1):7-15 [8837676] Epidemiol Rev. 1996;18(2):218-34 [9021314] Epidemiology. 1998 Jan;9(1):48-55 [9430268] J Occup Environ Med. 1998 Oct;40(10):855-61 [9800169] CA Cancer J Clin. 1999 Jan-Feb;49(1):33-64, 1 [10200776] Int J Cancer. 1999 Jun 11;81(6):871-6 [10362132] Eur J Cancer Prev. 1999 Jul;8(3):223-7 [10443951] Occup Environ Med. 1999 Nov;56(11):781-7 [10658565] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Occupational medicine: at a turning point or an expansion. AN - 67755939; 15837845 JF - Occupational and environmental medicine AU - Blair, A AD - blaira@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 VL - 62 IS - 5 KW - Index Medicus KW - Occupational Health KW - Culture KW - Humans KW - Neoplasms -- epidemiology KW - Occupational Exposure -- adverse effects KW - Risk Assessment -- methods KW - Neoplasms -- etiology KW - Occupational Diseases -- etiology KW - Occupational Diseases -- psychology KW - Occupational Diseases -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67755939?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Occupational+and+environmental+medicine&rft.atitle=Occupational+medicine%3A+at+a+turning+point+or+an+expansion.&rft.au=Blair%2C+A&rft.aulast=Blair&rft.aufirst=A&rft.date=2005-05-01&rft.volume=62&rft.issue=5&rft.spage=285%3B+discussion+287&rft.isbn=&rft.btitle=&rft.title=Occupational+and+environmental+medicine&rft.issn=1470-7926&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment On: Occup Environ Med. 2005 May;62(5):281-3 [15837843] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ephedrine plus caffeine causes age-dependent cardiovascular responses in Fischer 344 rats. AN - 67754760; 15653753 AB - Human consumption of ephedrine and caffeine in dietary supplements has been associated with a number of adverse effects including changes in the ECG, myocardial infarction, hyperthermia, and, in rare instances, death. The purpose of this study was to investigate the potential mechanisms associated with the cardiotoxicity of combined ephedrine and caffeine ingestion. Seven- and fourteen-week-old Fischer 344 rats treated with ephedrine in combination with caffeine exhibited increases in heart rate (HR), temperature, and corrected QT interval. Of the 14-wk-old rats treated with 25 mg/kg ephedrine plus 30 mg/kg caffeine, 57% died within 3-5 h of treatment, whereas none of the similarly treated 7-wk-old rats nor any of the rats treated with vehicle died. One hour after treatment with this dose of ephedrine plus caffeine, 14-wk-old rats exhibited a larger increase in HR (as % increase over baseline) than 7-wk-old rats. Furthermore, the 14-wk-old rats that died had a higher HR and temperature than the 14-wk-old rats that lived. Histopathological studies suggested interstitial hemorrhage and myofiber necrosis in the 14-wk-old rats treated with the highest concentration of ephedrine and caffeine. This study showed enhanced susceptibility to ephedrine plus caffeine in 14-wk-old rats compared with 7-wk-old rats. The greater mortality in the 14-wk-old rats was associated with increases in body temperature, HR, and myocardial necrosis. JF - American journal of physiology. Heart and circulatory physiology AU - Howden, Reuben AU - Hanlon, Paul R AU - Petranka, John G AU - Kleeberger, Steven AU - Bucher, John AU - Dunnick, June AU - Nyska, Abraham AU - Murphy, Elizabeth AD - Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - H2219 EP - H2224 VL - 288 IS - 5 SN - 0363-6135, 0363-6135 KW - Central Nervous System Stimulants KW - 0 KW - Caffeine KW - 3G6A5W338E KW - Ephedrine KW - GN83C131XS KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Age Factors KW - Body Temperature -- drug effects KW - In Vitro Techniques KW - Papillary Muscles -- drug effects KW - Drug Synergism KW - Papillary Muscles -- pathology KW - Male KW - Electrocardiography -- drug effects KW - Fever -- chemically induced KW - Heart Rate -- drug effects KW - Fever -- pathology KW - Caffeine -- toxicity KW - Central Nervous System Stimulants -- toxicity KW - Fever -- mortality KW - Ephedrine -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67754760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+physiology.+Heart+and+circulatory+physiology&rft.atitle=Ephedrine+plus+caffeine+causes+age-dependent+cardiovascular+responses+in+Fischer+344+rats.&rft.au=Howden%2C+Reuben%3BHanlon%2C+Paul+R%3BPetranka%2C+John+G%3BKleeberger%2C+Steven%3BBucher%2C+John%3BDunnick%2C+June%3BNyska%2C+Abraham%3BMurphy%2C+Elizabeth&rft.aulast=Howden&rft.aufirst=Reuben&rft.date=2005-05-01&rft.volume=288&rft.issue=5&rft.spage=H2219&rft.isbn=&rft.btitle=&rft.title=American+journal+of+physiology.+Heart+and+circulatory+physiology&rft.issn=03636135&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-31 N1 - Date created - 2005-04-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Advancing cancer biotherapy with proteomics. AN - 67754233; 15838374 AB - Proteomics is becoming increasingly important for cancer biotherapy. The development of high-throughput platforms now allows the analysis of multiple proteins from small quantities of material. While these techniques are being used to discover new biomarkers, they are particularly important for assessing complex biologic processes such as immunotherapy for cancer. Recent advances in this field are reviewed, as well as the use of proteomics to assess the effectiveness and toxicities of high-dose IL-2 cancer therapy. Proteomics is becoming useful in assessing cancer biotherapies and in unraveling their mechanisms of action. High-throughput proteomic technologies have now advanced to a stage where they have the potential to become effective discovery tools for biomarkers/predictors of disease, disease recurrence, and response to therapy. JF - Journal of immunotherapy (Hagerstown, Md. : 1997) AU - Stroncek, David F AU - Burns, Christine AU - Martin, Brian M AU - Rossi, Leonardo AU - Marincola, Francesco M AU - Panelli, Monica C AD - Immunogenetics Section, Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1148, USA. dstroncek@cc.nih.gov PY - 2005 SP - 183 EP - 192 VL - 28 IS - 3 SN - 1524-9557, 1524-9557 KW - Interleukin-2 KW - 0 KW - Index Medicus KW - Humans KW - Proteomics -- methods KW - Interleukin-2 -- therapeutic use KW - Neoplasms -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67754233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunotherapy+%28Hagerstown%2C+Md.+%3A+1997%29&rft.atitle=Advancing+cancer+biotherapy+with+proteomics.&rft.au=Stroncek%2C+David+F%3BBurns%2C+Christine%3BMartin%2C+Brian+M%3BRossi%2C+Leonardo%3BMarincola%2C+Francesco+M%3BPanelli%2C+Monica+C&rft.aulast=Stroncek&rft.aufirst=David&rft.date=2005-05-01&rft.volume=28&rft.issue=3&rft.spage=183&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunotherapy+%28Hagerstown%2C+Md.+%3A+1997%29&rft.issn=15249557&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-03 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - RhoA signaling is required for respiratory syncytial virus-induced syncytium formation and filamentous virion morphology. AN - 67744070; 15827147 AB - Respiratory syncytial virus (RSV) is an important human pathogen that can cause severe and life-threatening respiratory infections in infants, the elderly, and immunocompromised adults. RSV infection of HEp-2 cells induces the activation of RhoA, a small GTPase. We therefore asked whether RhoA signaling is important for RSV replication or syncytium formation. The treatment of HEp-2 cells with Clostridium botulinum C3, an enzyme that ADP-ribosylates and specifically inactivates RhoA, inhibited RSV-induced syncytium formation and cell-to-cell fusion, although similar levels of PFU were released into the medium and viral protein expression levels were equivalent. Treatment with another inhibitor of RhoA signaling, the Rho kinase inhibitor Y-27632, yielded similar results. Scanning electron microscopy of C3-treated infected cells showed reduced numbers of single blunted filaments, in contrast to the large clumps of long filaments in untreated infected cells. These data suggest that RhoA signaling is associated with filamentous virus morphology, cell-to-cell fusion, and syncytium formation but is dispensable for the efficient infection and production of infectious virus in vitro. Next, we developed a semiquantitative method to measure spherical and filamentous virus particles by using sucrose gradient velocity sedimentation. Fluorescence and transmission electron microscopy confirmed the separation of spherical and filamentous forms of infectious virus into two identifiable peaks. The C3 treatment of RSV-infected cells resulted in a shift to relatively more spherical virions than those from untreated cells. These data suggest that viral filamentous protuberances characteristic of RSV infection are associated with RhoA signaling, are important for filamentous virion morphology, and may play a role in initiating cell-to-cell fusion. JF - Journal of virology AU - Gower, Tara L AU - Pastey, Manoj K AU - Peeples, Mark E AU - Collins, Peter L AU - McCurdy, Lewis H AU - Hart, Timothy K AU - Guth, Alex AU - Johnson, Teresa R AU - Graham, Barney S AD - Vaccine Research Center, Building 40, Room 2502, NIAID, NIH, 40 Convent Dr., MSC 3017, Bethesda, MD 20892-3017, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 5326 EP - 5336 VL - 79 IS - 9 SN - 0022-538X, 0022-538X KW - Amides KW - 0 KW - Pyridines KW - Y 27632 KW - 138381-45-0 KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - exoenzyme C3, Clostridium botulinum KW - Botulinum Toxins KW - EC 3.4.24.69 KW - rhoA GTP-Binding Protein KW - EC 3.6.5.2 KW - Index Medicus KW - Virus Replication KW - Giant Cells -- ultrastructure KW - Amides -- pharmacology KW - Humans KW - Botulinum Toxins -- pharmacology KW - ADP Ribose Transferases -- pharmacology KW - Pyridines -- pharmacology KW - Cell Line KW - Respiratory Syncytial Viruses -- drug effects KW - rhoA GTP-Binding Protein -- metabolism KW - Respiratory Syncytial Viruses -- physiology KW - Respiratory Syncytial Viruses -- ultrastructure KW - Signal Transduction KW - rhoA GTP-Binding Protein -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67744070?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=RhoA+signaling+is+required+for+respiratory+syncytial+virus-induced+syncytium+formation+and+filamentous+virion+morphology.&rft.au=Gower%2C+Tara+L%3BPastey%2C+Manoj+K%3BPeeples%2C+Mark+E%3BCollins%2C+Peter+L%3BMcCurdy%2C+Lewis+H%3BHart%2C+Timothy+K%3BGuth%2C+Alex%3BJohnson%2C+Teresa+R%3BGraham%2C+Barney+S&rft.aulast=Gower&rft.aufirst=Tara&rft.date=2005-05-01&rft.volume=79&rft.issue=9&rft.spage=5326&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-03 N1 - Date created - 2005-04-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1999 Jul 23;274(30):21430-6 [10409706] Virology. 1996 Mar 1;217(1):76-87 [8599238] J Virol. 2000 Jan;74(1):228-36 [10590110] J Virol. 2000 Apr;74(7):3264-72 [10708443] J Gen Virol. 2000 May;81(Pt 5):1305-12 [10769073] J Virol. 2000 May;74(10):4634-44 [10775599] Blood. 2000 May 15;95(10):3044-51 [10807767] Virology. 2000 Jun 5;271(2):264-75 [10860881] Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14172-7 [11106388] Virology. 2001 May 10;283(2):188-96 [11336544] J Virol. 2002 Apr;76(8):3952-64 [11907235] J Gen Virol. 2002 Aug;83(Pt 8):1841-50 [12124448] J Virol. 2003 Feb;77(3):1747-56 [12525608] J Virol. 2003 Mar;77(6):3785-98 [12610153] Arch Gesamte Virusforsch. 1973;41(3):248-58 [4353798] J Virol. 1973 Nov;12(5):1173-80 [4128827] J Gen Virol. 1979 Aug;44(2):479-91 [118236] Arch Virol. 1980;63(3-4):275-84 [6766713] J Med Virol. 1979;4(3):201-11 [94087] J Cell Biol. 1987 Oct;105(4):1473-8 [3312229] AIDS Res Hum Retroviruses. 1987 Fall;3(3):245-52 [3501726] J Cell Biol. 1988 Nov;107(5):1689-95 [3182934] J Med Virol. 1988 Oct;26(2):153-62 [3183639] J Biol Chem. 1989 May 25;264(15):8602-5 [2498316] Membr Biochem. 1987-1988;7(4):231-47 [2855807] J Gen Virol. 1990 Dec;71 ( Pt 12):3075-80 [2177097] Cell. 1991 Mar 8;64(5):915-25 [1900457] J Gen Virol. 1991 Dec;72 ( Pt 12):3095-101 [1765771] Cell. 1992 Aug 7;70(3):389-99 [1643657] Nature. 1993 Mar 25;362(6418):318-24 [8455717] Cell. 1993 Nov 5;75(3):409-18 [8221884] Trends Microbiol. 1993 Nov;1(8):287-8 [8162412] Cell. 1996 Mar 22;84(6):941-51 [8601317] J Virol. 1996 Jul;70(7):4502-8 [8676475] J Biochem. 1996 Aug;120(2):215-28 [8889802] Mol Biol Cell. 1996 Nov;7(11):1825-34 [8930903] Nihon Yakurigaku Zasshi. 1997 Jan;109(1):13-7 [9067995] Trends Microbiol. 1997 Apr;5(4):142-8 [9141188] J Gen Virol. 1997 Aug;78 ( Pt 8):1885-9 [9266983] Nature. 1997 Oct 30;389(6654):990-4 [9353125] Virology. 1998 Jan 5;240(1):127-37 [9448697] Mol Biol Cell. 1998 Feb;9(2):403-19 [9450964] Proc Natl Acad Sci U S A. 1998 May 12;95(10):5746-51 [9576955] Science. 1998 Jun 26;280(5372):2074-5 [9669963] Virology. 1998 Aug 15;248(1):20-34 [9705252] J Virol. 1998 Dec;72(12):9561-6 [9811689] J Biol Chem. 1998 Dec 25;273(52):34663-6 [9856983] Virology. 1998 Dec 5;252(1):137-48 [9875324] Virology. 1994 May 1;200(2):801-5 [8178462] J Cell Biol. 1994 Jun;125(6):1371-84 [8207064] Cell. 1994 Sep 23;78(6):937-48 [7923363] J Cell Sci. 1995 Sep;108 ( Pt 9):3127-35 [8537452] Exp Cell Res. 1999 Jan 10;246(1):83-90 [9882517] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mechanisms of exocrine pancreatic toxicity induced by oral treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin in female Harlan Sprague-Dawley Rats. AN - 67740681; 15716480 AB - In previous 2-year studies of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) conducted by the National Toxicology Program on female Harlan Sprague-Dawley rats, acinar-cell vacuolation, atrophy, inflammation, and arteritis developed at high incidence, and a rare occurrence of pancreatic acinar-cell adenomas and carcinomas was noted. In this investigation, we sought to identify the mechanism involved in the early formative stages of acinar-cell lesions. Pancreas from animals treated for 14 and 31 weeks with 100 ng TCDD/kg body weight or corn oil vehicle was examined immunohistochemically and/or morphometrically. Acinar-cell kinetics were analyzed using staining with hematoxylin and eosin and proliferating cell nuclear antigen. Expressions of cytochrome P450 (CYP) 1A1 and aryl hydrocarbon receptor (AhR) were evaluated to assess direct effects of TCDD exposure. The cholecystokinin-A receptor (CCK-A receptor; CCKAR) and duodenal cholecystokinin 8 (CCK) revealed the associations of dioxin treatment with hormonal changes. Amylase localization showed acinar structural changes that could affect enzymatic production. Increased apoptotic activity in acinar cells occurred in 14- and 31-week-treated animals, with an increase in proliferative activity in the latter. Also in the latter, in the vacuolated acinar cells, CYP1A1 was overexpressed, and statistically significant decreases in expressions of AhR, CCKAR, and amylase occurred. The intensity of CCKAR expression increased in nonvacuolated acinar cells; a decrease in the size of CCK-positive epithelial cells occurred in duodenum. Our findings indicate that dioxin-induced acinar-cell lesions might be related to a direct effect of TCDD on the pancreas. Increase in CYP1A1 and decrease in CCKAR expressions in vacuolated acinar cells may be involved in the pathogenesis of pancreatic lesions. Changes in the expression of CYP or CCKAR may have induced the acinar-cell tumors by initiating proliferation. JF - Toxicological sciences : an official journal of the Society of Toxicology AU - Yoshizawa, Katsuhiko AU - Marsh, Tiwanda AU - Foley, Julie F AU - Cai, Bo AU - Peddada, Shyamal AU - Walker, Nigel J AU - Nyska, Abraham AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 594 EP - 606 VL - 85 IS - 1 SN - 1096-6080, 1096-6080 KW - Environmental Pollutants KW - 0 KW - Polychlorinated Dibenzodioxins KW - Index Medicus KW - Rats, Inbred Strains KW - Rats KW - Administration, Oral KW - Animals KW - Rats, Sprague-Dawley KW - Duodenum -- drug effects KW - Duodenum -- metabolism KW - Duodenum -- enzymology KW - Immunohistochemistry KW - Female KW - Duodenum -- pathology KW - Environmental Pollutants -- toxicity KW - Pancreas, Exocrine -- metabolism KW - Pancreas, Exocrine -- pathology KW - Apoptosis -- drug effects KW - Polychlorinated Dibenzodioxins -- toxicity KW - Pancreas, Exocrine -- drug effects KW - Pancreas, Exocrine -- enzymology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67740681?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.atitle=Mechanisms+of+exocrine+pancreatic+toxicity+induced+by+oral+treatment+with+2%2C3%2C7%2C8-tetrachlorodibenzo-p-dioxin+in+female+Harlan+Sprague-Dawley+Rats.&rft.au=Yoshizawa%2C+Katsuhiko%3BMarsh%2C+Tiwanda%3BFoley%2C+Julie+F%3BCai%2C+Bo%3BPeddada%2C+Shyamal%3BWalker%2C+Nigel+J%3BNyska%2C+Abraham&rft.aulast=Yoshizawa&rft.aufirst=Katsuhiko&rft.date=2005-05-01&rft.volume=85&rft.issue=1&rft.spage=594&rft.isbn=&rft.btitle=&rft.title=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-04-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lorazepam reinstates punishment-suppressed remifentanil self-administration in rats. AN - 67730403; 15821953 AB - We recently described a reinstatement procedure that models relapse to drug abuse in cases where abstinence results from aversive consequences of drug use. The potential value of this punishment-based model of relapse depends on its sensitivity to relapse-inducing events that are ineffective in the widely used extinction-based model. It is known that certain drugs can have anti-punishment effects, but these drugs have not been tested in the punishment-based reinstatement procedure. Therefore, the effects of the benzodiazepine, lorazepam, were examined using punishment-based and extinction-based reinstatement procedures. Rats self-administered the opioid, remifentanil (4 microg/kg per infusion). Two punishment groups were trained with response-contingent footshock that suppressed baseline rates of responding to zero. In an extinction group, remifentanil delivery was discontinued, and baseline responding stabilized at a low rate (mean=0.06 responses/min). Lorazepam (0.08-10 mg/kg, IP) was given during test sessions with the shock contingency discontinued for both punishment groups. Remifentanil delivery was maintained during testing in one punishment group but not the other. Lorazepam reinstated self-administration responding in both punishment groups but not in the extinction group. Priming injections of heroin reinstated responding in both the punishment and extinction groups, but combining heroin and lorazepam did not enhance reinstatement. This is the first demonstration that a trigger for relapse may have different effects depending on whether aversive conditioning contributed to the achievement of abstinence. It may be important to consider potential anti-punishment effects of both abused drugs and therapeutic agents in the treatment of individuals with a history of drug abuse. JF - Psychopharmacology AU - Panlilio, Leigh V AU - Thorndike, Eric B AU - Schindler, Charles W AD - Preclinical Pharmacology Section, Behavioral Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, NIH, DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. lpanlili@intra.nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 374 EP - 382 VL - 179 IS - 2 SN - 0033-3158, 0033-3158 KW - Analgesics, Opioid KW - 0 KW - Anti-Anxiety Agents KW - Piperidines KW - Heroin KW - 70D95007SX KW - Lorazepam KW - O26FZP769L KW - remifentanil KW - P10582JYYK KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Heroin -- pharmacology KW - Self Administration KW - Dose-Response Relationship, Drug KW - Extinction, Psychological -- drug effects KW - Heroin Dependence -- psychology KW - Electroshock KW - Male KW - Piperidines -- pharmacology KW - Conditioning, Operant -- drug effects KW - Anti-Anxiety Agents -- pharmacology KW - Analgesics, Opioid -- pharmacology KW - Punishment KW - Substance-Related Disorders -- psychology KW - Lorazepam -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67730403?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Lorazepam+reinstates+punishment-suppressed+remifentanil+self-administration+in+rats.&rft.au=Panlilio%2C+Leigh+V%3BThorndike%2C+Eric+B%3BSchindler%2C+Charles+W&rft.aulast=Panlilio&rft.aufirst=Leigh&rft.date=2005-05-01&rft.volume=179&rft.issue=2&rft.spage=374&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Calorie restriction increases cigarette use in adult smokers. AN - 67723943; 15565433 AB - Cigarette smokers weigh less than nonsmokers, and smokers often gain weight when they quit. This is a major barrier to smoking cessation, especially among women. However, strict dieting is not recommended during smoking cessation out of concern that it might promote relapse. This concern derives, in part, from the observation that calorie restriction increases self-administration of drugs of abuse in animals. This relationship has never been experimentally demonstrated in humans. To evaluate whether calorie restriction increases cigarette smoking in humans. Seventeen (nine males, eight females) healthy, normal-weight smokers not attempting to quit were cycled in partially counterbalanced order, double-blind, through four diets-normal calorie (2,000-2,800 kcal/day), low calorie (700 kcal/day deficit), low-carbohydrate (CHO)/normal-calorie, and low-CHO/low-calorie-for 6 days per diet in an inpatient research ward. Smoking was assessed by cigarette counts, breath carbon monoxide (CO) levels, and cigarette craving. Compared with the normal-calorie diet, while on the low-calorie diet, subjects smoked 8% more cigarettes (P<0.02) and had 11% higher breath CO levels (P<0.01). The low-CHO/normal-calorie diet showed no significant effect on either variable, but there was a 15% increase in breath CO levels (P<0.05) on the low-CHO/low-calorie diet. There were no changes in self-reported cigarette craving or mood. Consistent with animal studies, moderate calorie restriction was associated with a small but statistically significant increase in cigarette smoking, with no independent effect of CHO deprivation. These findings suggest that dieting may increase smoking behavior and could impede smoking-cessation attempts. JF - Psychopharmacology AU - Cheskin, Lawrence J AU - Hess, Judith M AU - Henningfield, Jack AU - Gorelick, David A AD - Department of Health and Human Services, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 430 EP - 436 VL - 179 IS - 2 SN - 0033-3158, 0033-3158 KW - Dietary Carbohydrates KW - 0 KW - Carbon Monoxide KW - 7U1EE4V452 KW - Index Medicus KW - Affect -- drug effects KW - Affect -- physiology KW - Double-Blind Method KW - Sex Characteristics KW - Humans KW - Prospective Studies KW - Adult KW - Tobacco Use Disorder -- psychology KW - Carbon Monoxide -- metabolism KW - Diet KW - Female KW - Male KW - Energy Intake -- physiology KW - Smoking -- metabolism KW - Diet, Reducing KW - Smoking -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67723943?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Calorie+restriction+increases+cigarette+use+in+adult+smokers.&rft.au=Cheskin%2C+Lawrence+J%3BHess%2C+Judith+M%3BHenningfield%2C+Jack%3BGorelick%2C+David+A&rft.aulast=Cheskin&rft.aufirst=Lawrence&rft.date=2005-05-01&rft.volume=179&rft.issue=2&rft.spage=430&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protection in the aged heart: preventing the heart-break of old age? AN - 67722627; 15820192 AB - The aged heart has a diminished functional and adaptive reserve capacity, an increased susceptibility to incur damage (e.g., as a result of ischemia), and a limited practical ability for repair/regeneration. Thus, there has been considerable interest to harness the heart's endogenous capacity to resist such damage, known as ischemic preconditioning (IPC), as well as other cardioprotective mechanisms. However, the translation of basic research findings into clinical practice has largely been inadequate because there have been few if any successful implementations in terms of viable therapies activating cardioprotection mechanisms to limit infarct size. Here, we provide an overview of the general mechanisms of cardioprotection, changes in the structure and function of the aged heart, and the current knowledge regarding cardioprotection in aged heart. The problems and opportunities for successful bench-to-bedside translation of cardioprotection in the elderly are discussed. JF - Cardiovascular research AU - Juhaszova, Magdalena AU - Rabuel, Christophe AU - Zorov, Dmitry B AU - Lakatta, Edward G AU - Sollott, Steven J AD - Laboratory of Cardiovascular Sciences, Gerontology Research Center, Box 13 Intramural Research Program, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Dr., Baltimore, MD 21224-6825, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 233 EP - 244 VL - 66 IS - 2 SN - 0008-6363, 0008-6363 KW - Cardiotonic Agents KW - 0 KW - Reactive Oxygen Species KW - Glycogen Synthase Kinase 3 KW - EC 2.7.11.26 KW - Index Medicus KW - Models, Animal KW - Cardiotonic Agents -- adverse effects KW - Signal Transduction -- physiology KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Myocardial Reperfusion KW - Humans KW - Cardiotonic Agents -- therapeutic use KW - Aged KW - Mitochondria, Heart -- metabolism KW - Glycogen Synthase Kinase 3 -- antagonists & inhibitors KW - Aging -- physiology KW - Ischemic Preconditioning, Myocardial KW - Myocardial Ischemia -- metabolism KW - Myocardium -- ultrastructure KW - Myocardial Ischemia -- prevention & control KW - Myocardium -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67722627?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cardiovascular+research&rft.atitle=Protection+in+the+aged+heart%3A+preventing+the+heart-break+of+old+age%3F&rft.au=Juhaszova%2C+Magdalena%3BRabuel%2C+Christophe%3BZorov%2C+Dmitry+B%3BLakatta%2C+Edward+G%3BSollott%2C+Steven+J&rft.aulast=Juhaszova&rft.aufirst=Magdalena&rft.date=2005-05-01&rft.volume=66&rft.issue=2&rft.spage=233&rft.isbn=&rft.btitle=&rft.title=Cardiovascular+research&rft.issn=00086363&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-01 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prenatal diethylstilbestrol (DES) exposure is associated with uterine leiomyoma development. AN - 67707578; 15808789 AB - Early life exposure to DES causes uterine leiomyomata in laboratory animals. We examined the relationship between prenatal DES exposure and development of uterine leiomyomata in women. Among randomly selected study participants (819 black women, 504 white women), leiomyoma status was determined by ultrasound screening (70%) or surgical record review (7%). We relied on self-report of prior diagnosis in 13%. Leiomyoma status could not be ascertained for 10% and they were excluded from analyses. Prenatal DES exposure was assessed by interview. All five of the black women who reported DES exposure had leiomyomata. Among white women, 76% who reported prenatal DES exposure had leiomyomata compared with 52% of the unexposed (adjusted odds ratio for whites: 2.4; 95% confidence interval CI: 1.1-5.4). Exposed women tended to have larger tumors. Results were robust to sensitivity analyses. Findings support experimental animal data and indicate a role for prenatal estrogen exposure in the etiology of human uterine leiomyoma. JF - Reproductive toxicology (Elmsford, N.Y.) AU - Baird, Donna Day AU - Newbold, Retha AD - Epidemiology Branch, A3-05, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, P.O. Box 12233, Research Triangle Park, NC 27709, USA. baird@niehs.nih.gov PY - 2005 SP - 81 EP - 84 VL - 20 IS - 1 SN - 0890-6238, 0890-6238 KW - Carcinogens KW - 0 KW - Diethylstilbestrol KW - 731DCA35BT KW - Index Medicus KW - Odds Ratio KW - Humans KW - Adult KW - District of Columbia -- epidemiology KW - Middle Aged KW - Female KW - Pregnancy KW - Uterine Neoplasms -- epidemiology KW - Maternal Exposure -- adverse effects KW - Leiomyoma -- epidemiology KW - Diethylstilbestrol -- adverse effects KW - Leiomyoma -- pathology KW - Uterine Neoplasms -- chemically induced KW - Leiomyoma -- chemically induced KW - Uterine Neoplasms -- pathology KW - Prenatal Exposure Delayed Effects KW - Carcinogens -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67707578?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.atitle=Prenatal+diethylstilbestrol+%28DES%29+exposure+is+associated+with+uterine+leiomyoma+development.&rft.au=Baird%2C+Donna+Day%3BNewbold%2C+Retha&rft.aulast=Baird&rft.aufirst=Donna&rft.date=2005-05-01&rft.volume=20&rft.issue=1&rft.spage=81&rft.isbn=&rft.btitle=&rft.title=Reproductive+toxicology+%28Elmsford%2C+N.Y.%29&rft.issn=08906238&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-26 N1 - Date created - 2005-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protein-protein interactions and posttranslational modifications in mammalian base excision repair. AN - 67705272; 15808410 AB - Base excision repair (BER) averts the cytotoxic and mutagenic effects of most endogenously produced DNA damage, including lesions that arise spontaneously due to the intrinsic instability of DNA or modifications that are formed from reactions with intracellular chemicals, such as reactive oxygen species and alkylating agents. Defects in the BER process have been associated with cancer susceptibility and neurodegenerative disorders. In its most simplistic form, BER can be fully reconstituted with a minimum of four human proteins and is completed in just five sequential steps: (i) excision of an inappropriate base by a DNA glycosylase (e.g., uracil DNA glycosylase); (ii) incision of the DNA backbone immediately adjacent to the resulting abasic site by apurinic/apyrimidimic endonuclease 1; (iii) removal of the 5'-abasic terminal fragment, and (iv) repair synthesis to fill the gap by DNA polymerase beta; and (v) ligation to seal the remaining nick by DNA ligase 1 or a complex of DNA ligase 3 and X-ray repair cross-complementing 1. However, BER can involve the participation of other proteins as well, such as alternative DNA polymerases or one of several nonessential "auxiliary" factors. In addition, BER operates most efficiently when specific protein-protein coordination occurs. Furthermore, several BER protein activities have been shown to be regulated by posttranslational modification, and some of the physical protein interactions link BER to other DNA transaction pathways. In this review, we summarize the current state of the emerging complexities of mammalian BER, focusing on the growing number of reported protein-protein interactions and posttranslational modifications. JF - Free radical biology & medicine AU - Fan, Jinshui AU - Wilson, David M AD - Laboratory of Molecular Gerontology, GRC, National Institute on Aging, IRP, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 1121 EP - 1138 VL - 38 IS - 9 SN - 0891-5849, 0891-5849 KW - Proteins KW - 0 KW - Index Medicus KW - Animals KW - Mammals KW - Protein Binding KW - DNA Repair KW - Protein Processing, Post-Translational KW - Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67705272?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Protein-protein+interactions+and+posttranslational+modifications+in+mammalian+base+excision+repair.&rft.au=Fan%2C+Jinshui%3BWilson%2C+David+M&rft.aulast=Fan&rft.aufirst=Jinshui&rft.date=2005-05-01&rft.volume=38&rft.issue=9&rft.spage=1121&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-16 N1 - Date created - 2005-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Importance of the surface area ratio on cytokines production by human monocytes in vitro induced by various hydroxyapatite particles. AN - 67336617; 15585239 AB - A possible complication associated with the implantation of hydroxyapatite (HA)-based prosthesis is the release of particles. Those particles can be phagocyted by monocytes that are among the first cells to colonize the inflammatory site. The activated monocytes produce inflammatory mediators, such as cytokines, which cause osteoclasts activation. It has previously been demonstrated using a surface area ratio (ratio of the total surface of the given particles to the surface area of cells) of 1 to 1 that there was a correlation between the expression and production of cytokines induced by HA. The present work studies the effect of physical characteristics of HA particles on the production of various inflammatory cytokines (tumour necrosis factor-alpha, interleukin (IL)-6, and IL-8) and anti-inflammatory cytokine (IL-10). However, the experiments were performed using a surface area ratio of 10 to 1. Our data demonstrate that all the particles, whatever their characteristics, induced a high expression of cytokines but the production was different, meaning that there was a post-transcriptional regulation. The size and sintering temperature seemed to be a characteristics that were less important compared to the shape; the needle particles appeared to induce the most important production of all the cytokines studied. JF - Biomaterials AU - Grandjean-Laquerriere, Alexia AU - Laquerriere, Patrice AU - Guenounou, Moncef AU - Laurent-Maquin, Dominique AU - Phillips, Terry M AD - Ultramicro Analytical Immunochemistry Resource, Division of Bioengineering and Physical Science, Office of Research Services, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2361 EP - 2369 VL - 26 IS - 15 SN - 0142-9612, 0142-9612 KW - Biocompatible Materials KW - 0 KW - Cytokines KW - Durapatite KW - 91D9GV0Z28 KW - Index Medicus KW - Dose-Response Relationship, Drug KW - Cells, Cultured KW - Particle Size KW - Humans KW - Materials Testing KW - Surface Properties KW - Durapatite -- adverse effects KW - Monocytes -- immunology KW - Biocompatible Materials -- adverse effects KW - Monocytes -- metabolism KW - Cytokines -- immunology KW - Monocytes -- drug effects KW - Cytokines -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67336617?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomaterials&rft.atitle=Importance+of+the+surface+area+ratio+on+cytokines+production+by+human+monocytes+in+vitro+induced+by+various+hydroxyapatite+particles.&rft.au=Grandjean-Laquerriere%2C+Alexia%3BLaquerriere%2C+Patrice%3BGuenounou%2C+Moncef%3BLaurent-Maquin%2C+Dominique%3BPhillips%2C+Terry+M&rft.aulast=Grandjean-Laquerriere&rft.aufirst=Alexia&rft.date=2005-05-01&rft.volume=26&rft.issue=15&rft.spage=2361&rft.isbn=&rft.btitle=&rft.title=Biomaterials&rft.issn=01429612&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2004-12-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Initial Evaluation of the Effects of Aerosolized Florida Red Tide Toxins (Brevetoxins) in Persons with Asthma AN - 21356477; 7683876 AB - Florida red tides annually occur in the Gulf of Mexico, resulting from blooms of the marine dinoflagellate Karenia brevis. K. brevis produces highly potent natural polyether toxins, known as brevetoxins, that activate voltage-sensitive sodium channels. In experimental animals, brevetoxins cause significant bronchoconstriction. A study of persons who visited the beach recreationally found a significant increase in self-reported respiratory symptoms after exposure to aerosolized Florida red tides. Anecdotal reports indicate that persons with underlying respiratory diseases may be particularly susceptible to adverse health effects from these aerosolized toxins. Fifty-nine persons with physician-diagnosed asthma were evaluated for 1 hr before and after going to the beach on days with and without Florida red tide. Study participants were evaluated with a brief symptom questionnaire, nose and throat swabs, and spirometry approved by the National Institute for Occupational Safety and Health. Environmental monitoring, water and air sampling (i.e., K. brevis, brevetoxins, and particulate size distribution), and personal monitoring (for toxins) were performed. Brevetoxin concentrations were measured by liquid chromatography mass spectrometry, high-performance liquid chromatography, and a newly developed brevetoxin enzyme-linked immunosorbent assay. Participants were significantly more likely to report respiratory symptoms after Florida red tide exposure. Participants demonstrated small but statistically significant decreases in forced expiratory volume in 1 sec, forced expiratory flow between 25 and 75%, and peak expiratory flow after exposure, particularly those regularly using asthma medications. Similar evaluation during nonexposure periods did not significantly differ. This is the first study to show objectively measurable adverse health effects from exposure to aerosolized Florida red tide toxins in persons with asthma. Future studies will examine the possible chronic effects of these toxins among persons with asthma and other chronic respiratory impairment. JF - Environmental Health Perspectives AU - Fleming, Lora E AU - Kirkpatrick, Barbara AU - Backer, Lorraine C AU - Bean, Judy A AU - Wanner, Adam AU - Dalpra, Dana AU - Tamer, Robert AU - Zaias, Julia AU - Cheng, Yung Sung AU - Pierce, Richard AU - Naar, Jerome AU - Abraham, William AU - Clark, Richard AU - Zhou, Yue AU - Henry, Michael S AU - Johnson, David AU - Van De Bogart, Gayl AU - Bossart, Gregory D AU - Harrington, Mark AU - Baden, Daniel G AD - super(1)National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Sciences Center, University of Miami Rosenstiel School of Marine and Atmospheric Sciences, Miami, Florida, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 650 EP - 657 PB - US Government Printing Office, Superintendent of Documents, P.O. Box 371954 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Pollution Abstracts KW - Red tides KW - Occupational safety KW - Mass spectrometry KW - Respiratory diseases KW - Dinoflagellates KW - Air sampling KW - Drugs KW - Environmental monitoring KW - ASW, USA, Florida KW - Beaches KW - Particulate size KW - Asthma KW - Toxins KW - Channels KW - Sodium KW - ASW, Mexico Gulf KW - Liquid chromatography KW - Karenia brevis KW - Immunoassays KW - P 0000:AIR POLLUTION UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21356477?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Initial+Evaluation+of+the+Effects+of+Aerosolized+Florida+Red+Tide+Toxins+%28Brevetoxins%29+in+Persons+with+Asthma&rft.au=Fleming%2C+Lora+E%3BKirkpatrick%2C+Barbara%3BBacker%2C+Lorraine+C%3BBean%2C+Judy+A%3BWanner%2C+Adam%3BDalpra%2C+Dana%3BTamer%2C+Robert%3BZaias%2C+Julia%3BCheng%2C+Yung+Sung%3BPierce%2C+Richard%3BNaar%2C+Jerome%3BAbraham%2C+William%3BClark%2C+Richard%3BZhou%2C+Yue%3BHenry%2C+Michael+S%3BJohnson%2C+David%3BVan+De+Bogart%2C+Gayl%3BBossart%2C+Gregory+D%3BHarrington%2C+Mark%3BBaden%2C+Daniel+G&rft.aulast=Fleming&rft.aufirst=Lora&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=650&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7500 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-02-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Karenia brevis; ASW, USA, Florida; ASW, Mexico Gulf; Respiratory diseases; Red tides; Toxins; Asthma; Liquid chromatography; Beaches; Sodium; Channels; Particulate size; Environmental monitoring; Air sampling; Dinoflagellates; Mass spectrometry; Immunoassays; Occupational safety; Drugs DO - http://dx.doi.org/10.1289/ehp.7500 ER - TY - JOUR T1 - Evidence that HIV-1 Encodes an siRNA and a Suppressor of RNA Silencing AN - 21138615; 6658718 AB - In plants and invertebrate animals, RNA silencing is a form of nucleic acid- based adaptive immunity. By contrast, jawed vertebrates have evolved complex protein-based adaptive immunity. Although short interfering RNAs (siRNAs) have been used as artificial tools to silence viral infection in human cells, it remains unknown whether mammalian viruses naturally elicit such immunity in vertebral cells. Here, we report the evidence that HIV-1 encodes viral siRNA precursors in its genome and that natural HIV-1 infection provokes nucleic acid- based immunity in human cells. To combat this cellular defense, HIV-1 has evolved in its Tat protein a suppressor of RNA silencing (SRS) function. Tat abrogates the cell's RNA-silencing defense by subverting the ability of Dicer to process precursor double-stranded RNAs into siRNAs. JF - Immunity AU - Bennasser, Yamina AU - Le, Shu-Yun AU - Benkirane, Monsef AU - Jeang, Kuan-Teh AD - Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, kj7e@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 607 EP - 619 PB - Cell Press, 1100 Massachusetts Avenue Cambridge MA 02138 USA, [mailto:subs@cell.com], [URL:http://www.cellpress.com] VL - 22 IS - 5 SN - 1074-7613, 1074-7613 KW - HIV-1 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Immunology Abstracts; Virology & AIDS Abstracts KW - Genomes KW - siRNA KW - Tat protein KW - Double-stranded RNA KW - Human immunodeficiency virus 1 KW - RNA-mediated interference KW - Immunity KW - Infection KW - Vertebrae KW - V 22002:AIDS: Molecular and in vitro aspects KW - W 30940:Products KW - N 14830:RNA KW - F 06104:Virus UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21138615?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Immunity&rft.atitle=Evidence+that+HIV-1+Encodes+an+siRNA+and+a+Suppressor+of+RNA+Silencing&rft.au=Bennasser%2C+Yamina%3BLe%2C+Shu-Yun%3BBenkirane%2C+Monsef%3BJeang%2C+Kuan-Teh&rft.aulast=Bennasser&rft.aufirst=Yamina&rft.date=2005-05-01&rft.volume=22&rft.issue=5&rft.spage=607&rft.isbn=&rft.btitle=&rft.title=Immunity&rft.issn=10747613&rft_id=info:doi/10.1016%2Fj.immuni.2005.03.010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Genomes; siRNA; Tat protein; Double-stranded RNA; RNA-mediated interference; Immunity; Infection; Vertebrae; Human immunodeficiency virus 1 DO - http://dx.doi.org/10.1016/j.immuni.2005.03.010 ER - TY - JOUR T1 - Fold recognition aided by constraints from small angle X-ray scattering data AN - 20857189; 6417236 AB - We performed a systematic exploration of the use of structural information derived from small angle X-ray scattering (SAXS) measurements to improve fold recognition. SAXS data provide the Fourier transform of the histogram of atomic pair distances (pair distribution function) for a given protein and hence can serve as a structural constraint on methods used to determine the native conformational fold of the protein. Here we used it to construct a similarity-based fitness score with which to evaluate candidate structures generated by a threading procedure. In order to combine the SAXS scores with the standard energy scores and other 1D profile-based scores used in threading, we made use both of a linear regression method and of a neural network-based technique to obtain optimal combined fitness scores and applied them to the ranking of candidate structures. Our results show that the use of SAXS data with gapless threading significantly improves the performance of fold recognition. JF - Protein Engineering Design and Selection AU - Zheng, Wenjun AU - Doniach, Sebastian AD - Departments of Physics and Applied Physics and Laboratory for Advanced Materials, Stanford University, CA 94305 and Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 209 EP - 219 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 18 IS - 5 SN - 1741-0126, 1741-0126 KW - Biotechnology and Bioengineering Abstracts KW - Fitness KW - Data processing KW - Protein folding KW - Energy KW - X-ray scattering KW - W 30925:Genetic Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20857189?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Engineering+Design+and+Selection&rft.atitle=Fold+recognition+aided+by+constraints+from+small+angle+X-ray+scattering+data&rft.au=Zheng%2C+Wenjun%3BDoniach%2C+Sebastian&rft.aulast=Zheng&rft.aufirst=Wenjun&rft.date=2005-05-01&rft.volume=18&rft.issue=5&rft.spage=209&rft.isbn=&rft.btitle=&rft.title=Protein+Engineering+Design+and+Selection&rft.issn=17410126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-09-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Data processing; Protein folding; Fitness; X-ray scattering; Energy ER - TY - JOUR T1 - Validation of tissue microarray immunohistochemistry staining and interpretation in diffuse large B-cell lymphoma AN - 20089464; 7382422 AB - Tissue microarrays (TMAs) show concordance with whole tissue sections in the immunohistochemical evaluation of tumor cells. However, potential inter- institutional variability among observers and immunohistochemical staining methods has not been fully addressed. We selected 21 cases of diffuse large B- cell lymphoma (DLBCL) to process for TMAs. Immunohistochemical stains were performed in 3 laboratories, and reviewed independently by 3 hematopathologists at the 3 institutions. Stains were scored on a 4-point scale. Statistical analyses of variation in the scoring among observers and among different institutions stains were performed. Stains for CD3, CD10, CD20, BCL-2, BCL-6, MIB-1, and FOX-P1 revealed little variation among observers, with an average 51?- ?82% complete agreement and 82?-?100% agreement? plus or minus ?1 numerical score. The rate of concordance when evaluating most stains performed in different laboratories was also relatively good, with an average of 55?-?72% complete agreement and 70?- ?97% agreement? plus or minus ?1 score. However, scoring of MUM-1 and p53 stains showed wider variation, with an average of only 37 and 30% complete agreement among observers, and 11 and 45% agreement when stains from different institutions were examined. Further statistical analyses were performed to compare the observers scoring of their own institutions stains (self-review) vs. observers scoring of other institutions stains (non-self). The agreement rate for the p53 stain was significantly higher when based on self-review (average 58% complete agreement) compared with an agreement rate of only 10.5% when based on a review of stains performed in another laboratory, non-self review, P ?-?0.01. This difference in the self- vs. non-self review was not seen when data for MUM-1 were analysed. In conclusion, most phenotypic markers used in the analysis of DLBCL can be evaluated in TMAs with adequate agreement among observers and laboratories. These include CD3, CD20, CD10, BCL-2, BCL-6, MIB-1, and FOX-P1. However, some markers, such as p53 and MUM-1, are more prone to inter- institutional variation. Variations in interpretation can be partially overcome by self-adjusted/adapt tendency, as seen with p53. Especially with newly developed markers, such as MUM-1, the development of standardized techniques for staining and interpretation is critical to reduce inter-observer variability. JF - Leukemia & Lymphoma AU - Zu, Youli AU - Steinberg, Seth AU - Campo, Elias AU - Hans, Christine AU - Weisenburger, Dennis AU - Braziel, Rita AU - Delabie, Jan AU - Gascoyne, Randy AU - Muller-Hermlink, Konrad AU - Pittaluga, Stefania AU - Raffeld, Mark AU - Chan, Wing AU - Jaffe, Elaine AD - Hematopathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 693 EP - 701 PB - Taylor & Francis Ltd., 11 New Fetter Lane London EC4P 4EE UK, [mailto:info@tandf.co.uk], [URL:http://www.tandf.co.uk] VL - 46 IS - 5 SN - 1042-8194, 1042-8194 KW - Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - Validation KW - monoclonal antibodies KW - tissue microarray KW - immunohistochemistry KW - diffuse large B-cell lymphoma KW - prognostic markers KW - B-cell lymphoma KW - double prime B-cell lymphoma KW - Data processing KW - Statistical analysis KW - Stains KW - Tumor cells KW - p53 protein KW - Leukemia KW - Bcl-6 protein KW - Reviews KW - CD20 antigen KW - Bcl-2 protein KW - CD3 antigen KW - Immunohistochemistry KW - F 06915:Cancer Immunology KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20089464?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Leukemia+%26+Lymphoma&rft.atitle=Validation+of+tissue+microarray+immunohistochemistry+staining+and+interpretation+in+diffuse+large+B-cell+lymphoma&rft.au=Zu%2C+Youli%3BSteinberg%2C+Seth%3BCampo%2C+Elias%3BHans%2C+Christine%3BWeisenburger%2C+Dennis%3BBraziel%2C+Rita%3BDelabie%2C+Jan%3BGascoyne%2C+Randy%3BMuller-Hermlink%2C+Konrad%3BPittaluga%2C+Stefania%3BRaffeld%2C+Mark%3BChan%2C+Wing%3BJaffe%2C+Elaine&rft.aulast=Zu&rft.aufirst=Youli&rft.date=2005-05-01&rft.volume=46&rft.issue=5&rft.spage=693&rft.isbn=&rft.btitle=&rft.title=Leukemia+%26+Lymphoma&rft.issn=10428194&rft_id=info:doi/10.1080%2F10428190500051844 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-05-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - B-cell lymphoma; Data processing; double prime B-cell lymphoma; Statistical analysis; Stains; Tumor cells; p53 protein; Leukemia; Bcl-6 protein; Reviews; CD20 antigen; CD3 antigen; Bcl-2 protein; Immunohistochemistry DO - http://dx.doi.org/10.1080/10428190500051844 ER - TY - JOUR T1 - Effect of Human Stem Cells Labeled with Ferumoxides-Poly-L-lysine on Hematologic and Biochemical Measurements in Rats AN - 19929920; 6276670 AB - PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner. [copy ] RSNA, 2005 JF - Radiology AU - Yocum, Gene T AU - Wilson, Lindsey B AU - Ashari, Parwana AU - Jordan, EKay AU - Frank, Joseph A AU - Arbab, Ali S AD - Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, Md Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 547 EP - 552 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 235 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts KW - Hemoglobin KW - Blood KW - Stem cells KW - Intravenous administration KW - Alkaline phosphatase KW - Renal function KW - Aspartate aminotransferase KW - Liver KW - Bilirubin KW - Mesenchyme KW - Iron KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19929920?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Effect+of+Human+Stem+Cells+Labeled+with+Ferumoxides-Poly-L-lysine+on+Hematologic+and+Biochemical+Measurements+in+Rats&rft.au=Yocum%2C+Gene+T%3BWilson%2C+Lindsey+B%3BAshari%2C+Parwana%3BJordan%2C+EKay%3BFrank%2C+Joseph+A%3BArbab%2C+Ali+S&rft.aulast=Yocum&rft.aufirst=Gene&rft.date=2005-05-01&rft.volume=235&rft.issue=2&rft.spage=547&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-07-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Hemoglobin; Blood; Intravenous administration; Stem cells; Alkaline phosphatase; Renal function; Aspartate aminotransferase; Liver; Bilirubin; Mesenchyme; Iron ER - TY - JOUR T1 - Standardizing global gene expression analysis between laboratories and across platforms AN - 19524308; 7924058 AB - To facilitate collaborative research efforts between multi-investigator teams using DNA microarrays, we identified sources of error and data variability between laboratories and across microarray platforms, and methods to accommodate this variability. RNA expression data were generated in seven laboratories, which compared two standard RNA samples using 12 microarray platforms. At least two standard microarray types (one spotted, one commercial) were used by all laboratories. Reproducibility for most platforms within any laboratory was typically good, but reproducibility between platforms and across laboratories was generally poor. Reproducibility between laboratories increased markedly when standardized protocols were implemented for RNA labeling, hybridization, microarray processing, data acquisition and data normalization. Reproducibility was highest when analysis was based on biological themes defined by enriched Gene Ontology (GO) categories. These findings indicate that microarray results can be comparable across multiple laboratories, especially when a common platform and set of procedures are used. JF - Nature Methods AU - Weis, B K AD - A list of authors and their affiliations appears in the Supplementary Note online., weis@niehs.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 351 EP - 356 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 2 IS - 5 SN - 1548-7091, 1548-7091 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - DNA microarrays KW - Data acquisition KW - Data processing KW - Gene expression KW - RNA KW - W 30900:Methods KW - G 07700:Molecular Genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19524308?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Methods&rft.atitle=Standardizing+global+gene+expression+analysis+between+laboratories+and+across+platforms&rft.au=Weis%2C+B+K&rft.aulast=Weis&rft.aufirst=B&rft.date=2005-05-01&rft.volume=2&rft.issue=5&rft.spage=351&rft.isbn=&rft.btitle=&rft.title=Nature+Methods&rft.issn=15487091&rft_id=info:doi/10.1038%2Fnmeth754 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2013-02-22 N1 - SubjectsTermNotLitGenreText - Gene expression; Data processing; RNA; DNA microarrays; Data acquisition DO - http://dx.doi.org/10.1038/nmeth754 ER - TY - JOUR T1 - A novel method to improve prenatal diagnosis of abnormal systemic venous connections using three- and four-dimensional ultrasonography and 'inversion mode' AN - 19436584; 6786704 AB - Objective: The precise prenatal diagnosis of abnormal venous connections of the fetal heart is challenging. Anatomical accuracy may be important in determining the best route for postnatal angiography, as well as the prognosis and treatment. This study was designed to determine the value of 'inversion mode', a new three- and four-dimensional (4D) rendering algorithm, in the visualization of the spatial relationships of an interrupted inferior vena cava (IVC) with azygos or hemiazygos vein continuation associated with and without heterotaxic syndromes. Methods: Heart volumes were acquired using 4D ultrasonography and spatiotemporal image correlation in cases of interrupted IVC with azygos/hemiazygos continuation (n = 3). Volume datasets were rendered using the 'inversion mode' algorithm and abnormal images were compared to those generated from a library of normal fetuses. Results: The 'inversion mode' rendering algorithm allowed the visualization of dilated azygos or hemiazygos veins and their spatial relationships with the descending aorta, the aortic arch, the superior vena cava, and the atria in cases of interrupted IVC with and without heterotaxic syndromes. Conclusions: The 'inversion mode' algorithm improves prenatal visualization of both dilated azygos and hemiazygos veins, as well as their spatial relationships with the surrounding vascular structures. This has implications for the accurate prenatal diagnosis and management of neonates with abnormal systemic venous connections. JF - Ultrasound in Obstetrics and Gynecology AU - Espinoza, J AU - Goncalves, L F AU - Lee, W AU - Mazor, M AU - Romero, R AD - Perinatology Research Branch, NICHD, NIH, DHHS, Wayne State University/Hutzel Women's Hospital, 3990 John R, 4th Floor, Detroit, MI 48201, USA, warfiela@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 428 EP - 434 PB - John Wiley & Sons, Ltd. VL - 25 IS - 5 SN - 0960-7692, 0960-7692 KW - Biotechnology and Bioengineering Abstracts KW - Heart KW - Gynecology KW - Aorta KW - Prognosis KW - Algorithms KW - Prenatal diagnosis KW - Ultrasonography KW - Fetuses KW - Angiography KW - Veins KW - Aortic arch KW - Inversion KW - Neonates KW - Obstetrics KW - Ultrasound KW - Vascular system KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19436584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Ultrasound+in+Obstetrics+and+Gynecology&rft.atitle=A+novel+method+to+improve+prenatal+diagnosis+of+abnormal+systemic+venous+connections+using+three-+and+four-dimensional+ultrasonography+and+%27inversion+mode%27&rft.au=Espinoza%2C+J%3BGoncalves%2C+L+F%3BLee%2C+W%3BMazor%2C+M%3BRomero%2C+R&rft.aulast=Espinoza&rft.aufirst=J&rft.date=2005-05-01&rft.volume=25&rft.issue=5&rft.spage=428&rft.isbn=&rft.btitle=&rft.title=Ultrasound+in+Obstetrics+and+Gynecology&rft.issn=09607692&rft_id=info:doi/10.1002%2Fuog.1877 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Inversion; Algorithms; Prenatal diagnosis; Veins; Ultrasonography; Fetuses; Heart; Neonates; Aortic arch; Ultrasound; Gynecology; Angiography; Obstetrics; Aorta; Vascular system; Prognosis DO - http://dx.doi.org/10.1002/uog.1877 ER - TY - JOUR T1 - Simultaneous perfusion and blood-oxygenation-level-dependent measurements using single-shot interleaved z-shim echo-planar imaging AN - 19430100; 6486294 AB - Single-shot interleaved z-shim EPI (SSIZS-EPI) was extended to a simultaneous perfusion and blood-oxygenation-level-dependent (BOLD) imaging technique that reduces susceptibility-induced signal loss while preserving rapid image acquisition. Experiments on human brains showed that images acquired with this technique had improved signal-to-noise ratio in the inferior prefrontal, meso-, and lateral-temporal lobes compared with a conventional EPI. Perfusion maps obtained from the SSIZS-EPI images at resting state illustrated substantial signal recovery in these brain areas. Perfusion and BOLD images collected with a sensorimotor paradigm demonstrated the feasibility of the technique to simultaneously measure cerebral blood flow and blood oxygenation signals associated with brain activation. Functional experiments with a neuropsychiatric paradigm showed increased brain activities in the periamygdalar regions in both perfusion and BOLD maps, consistent with a previous H sub(2) super(15)O PET study. The proposed technique, with its advantages of reducing susceptibility artifacts and fast scanning speed, would be useful for obtaining more reliable measurements of functional signals, particularly in the brain regions with field inhomogeneities. JF - Magnetic Resonance in Medicine AU - Yang, Yihong AU - Gu, Hong AU - Silbersweig, David A AU - Stern, Emily AD - Neuroimaging Research Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Building C, Room 383, Baltimore, MD 21042, USA, yihongyang@intra.nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1207 EP - 1211 PB - John Wiley & Sons, Ltd. VL - 53 IS - 5 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - sensorimotor system KW - Neuroimaging KW - Perfusion KW - Scanning KW - Functional magnetic resonance imaging KW - Magnetic resonance imaging KW - Positron emission tomography KW - Brain KW - Cerebral blood flow KW - Gene mapping KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19430100?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Simultaneous+perfusion+and+blood-oxygenation-level-dependent+measurements+using+single-shot+interleaved+z-shim+echo-planar+imaging&rft.au=Yang%2C+Yihong%3BGu%2C+Hong%3BSilbersweig%2C+David+A%3BStern%2C+Emily&rft.aulast=Yang&rft.aufirst=Yihong&rft.date=2005-05-01&rft.volume=53&rft.issue=5&rft.spage=1207&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20431 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Brain; Perfusion; Neuroimaging; Gene mapping; Scanning; sensorimotor system; Cerebral blood flow; Positron emission tomography; Magnetic resonance imaging; Functional magnetic resonance imaging DO - http://dx.doi.org/10.1002/mrm.20431 ER - TY - JOUR T1 - RESTORE: Robust estimation of tensors by outlier rejection AN - 19430041; 6486276 AB - Signal variability in diffusion-weighted imaging (DWI) is influenced by both thermal noise and spatially and temporally varying artifacts such as subject motion and cardiac pulsation. In this paper, the effects of DWI artifacts on estimated tensor values, such as trace and fractional anisotropy, are analyzed using Monte Carlo simulations. A novel approach for robust diffusion tensor estimation, called RESTORE (for robust estimation of tensors by outlier rejection), is proposed. This method uses iteratively reweighted least-squares regression to identify potential outliers and subsequently exclude them. Results from both simulated and clinical diffusion data sets indicate that the RESTORE method improves tensor estimation compared to the commonly used linear and nonlinear least-squares tensor fitting methods and a recently proposed method based on the German-McClure M-estimator. The RESTORE method could potentially remove the need for cardiac gating in DWI acquisitions and should be applicable to other MR imaging techniques that use univariate or multivariate regression to fit MRI data to a model. JF - Magnetic Resonance in Medicine AU - Chang, Lin-Ching AU - Jones, Derek K AU - Pierpaoli, Carlo AD - National Institutes of Health, Building 13, Room 3W16, 13 South Drive, Bethesda, MD 20892-5772, USA, cp1a@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1088 EP - 1095 PB - John Wiley & Sons, Ltd. VL - 53 IS - 5 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Monte Carlo simulation KW - Heart KW - Anisotropy KW - Gating KW - Magnetic resonance imaging KW - Regression analysis KW - Diffusion KW - N.M.R. KW - Models KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19430041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=RESTORE%3A+Robust+estimation+of+tensors+by+outlier+rejection&rft.au=Chang%2C+Lin-Ching%3BJones%2C+Derek+K%3BPierpaoli%2C+Carlo&rft.aulast=Chang&rft.aufirst=Lin-Ching&rft.date=2005-05-01&rft.volume=53&rft.issue=5&rft.spage=1088&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20426 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Heart; Magnetic resonance imaging; Models; N.M.R.; Diffusion; Regression analysis; Monte Carlo simulation; Anisotropy; Gating DO - http://dx.doi.org/10.1002/mrm.20426 ER - TY - JOUR T1 - Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects AN - 19424409; 6474789 AB - A new recombinant protein (NIE) for immunodiagnosis of human Strongyloides infection has 13% to 18% amino acid identity with antigen 5 insect venom allergen, but the C-terminal segment of NIE showed highest identity with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as bands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay. JF - American Journal of Tropical Medicine and Hygiene AU - Ravi, V AU - King, T P AU - Andersen, J F AU - Nutman, T B AU - Neva, F A AD - LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, USA, fneva@niaid.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 549 EP - 553 VL - 72 IS - 5 SN - 0002-9637, 0002-9637 KW - Hornets KW - Paper wasps KW - Potter wasps KW - Yellowjackets KW - Toxicology Abstracts; Entomology Abstracts KW - Western blotting KW - Enzyme-linked immunosorbent assay KW - Amino acids KW - Vespidae KW - Infection KW - Immunodiagnosis KW - Strongyloides stercoralis KW - Polistes KW - Allergens KW - Immunoglobulin E KW - Immunoglobulin G KW - Venom KW - Epitopes KW - Z 05350:Medical, Veterinary, and Agricultural Entomology KW - X 24173:Animals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19424409?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Tropical+Medicine+and+Hygiene&rft.atitle=Strongyloides+stercoralis+recombinant+NIE+antigen+shares+epitope+with+recombinant+Ves+v+5+and+Pol+a+5+allergens+of+insects&rft.au=Ravi%2C+V%3BKing%2C+T+P%3BAndersen%2C+J+F%3BNutman%2C+T+B%3BNeva%2C+F+A&rft.aulast=Ravi&rft.aufirst=V&rft.date=2005-05-01&rft.volume=72&rft.issue=5&rft.spage=549&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Tropical+Medicine+and+Hygiene&rft.issn=00029637&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Immunodiagnosis; Western blotting; Enzyme-linked immunosorbent assay; Amino acids; Immunoglobulin E; Allergens; Immunoglobulin G; Infection; Venom; Epitopes; Strongyloides stercoralis; Polistes; Vespidae ER - TY - JOUR T1 - Partial Redirection of Transgenic Human Growth Hormone Secretion from Rat Salivary Glands AN - 17886909; 6258662 AB - Regulated secretory pathway proteins, when delivered as transgenes to salivary glands, are secreted pre-dominantly into saliva. This is not useful for those proteins whose therapeutic function is required systemically, for example, human growth hormone (hGH). One strategy to improve the efficiency of hGH secretion into the bloodstream involves manipulation of existing sorting signals. The C terminus of hGH is highly conserved and contains a domain similar to the regulated pathway sorting domain of pro-opiomelanocortin (POMC). We hypothesized that, similar to POMC, mutation of this domain would divert hGH secretion from the regulated to the constitutive pathway, which in salivary glands leads to the bloodstream. Several mutations were made in the C terminus of the hGH cDNA and tested in vitro. One biologically active mutant containing E174A and E186A substitutions, and with an included C-terminal extension, was studied in greater detail. Compared with wild-type hGH, we found that this mutant hGH accumulated in the Golgi/trans-Golgi network and showed increased basal secretion in AtT20 cells, a model endocrine cell line. Importantly, in vivo, the mutant hGH displayed a relative increase in the proportion of constitutive pathway secretion seen from rat salivary glands, with a significantly lower saliva-versus-serum secretion ratio (p = 0.03). Although this mutant is unlikely to be therapeutically beneficial, these results suggest that the final destination of a transgenic secretory protein may be controlled by reengineering its sorting determinants. JF - Human Gene Therapy AU - Wang, J AU - Cawley, N X AU - Voutetakis, A AU - Rodriguez, Y M AU - Goldsmith, C M AU - Nieman, L K AU - Hoque, ATMS AU - Frank, S J AU - Snell, C R AU - Loh, Y P AU - Baum, B J AD - Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Building 10, Room 1N113, MSC-1190, Bethesda, MD 20892, USA, bbaum@dir.nidcr.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 571 EP - 583 VL - 16 IS - 5 SN - 1043-0342, 1043-0342 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Golgi apparatus KW - Growth hormone KW - Gene therapy KW - Proopiomelanocortin KW - Secretion KW - Animal models KW - Cell culture KW - Salivary gland KW - Saliva KW - Mutation KW - W 30965:Miscellaneous, Reviews KW - W3 33180:Gene based (protocols, clinical trials, and animal models) KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17886909?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+Gene+Therapy&rft.atitle=Partial+Redirection+of+Transgenic+Human+Growth+Hormone+Secretion+from+Rat+Salivary+Glands&rft.au=Wang%2C+J%3BCawley%2C+N+X%3BVoutetakis%2C+A%3BRodriguez%2C+Y+M%3BGoldsmith%2C+C+M%3BNieman%2C+L+K%3BHoque%2C+ATMS%3BFrank%2C+S+J%3BSnell%2C+C+R%3BLoh%2C+Y+P%3BBaum%2C+B+J&rft.aulast=Wang&rft.aufirst=J&rft.date=2005-05-01&rft.volume=16&rft.issue=5&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Human+Gene+Therapy&rft.issn=10430342&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Growth hormone; Secretion; Salivary gland; Proopiomelanocortin; Mutation; Animal models; Saliva; Cell culture; Golgi apparatus; Gene therapy ER - TY - JOUR T1 - Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Production Significantly Increases during Tricarboxylic Acid Cycle Stress AN - 17875150; 6245838 AB - Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing Staphylococcus epidermidis with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production. JF - Journal of Bacteriology AU - Vuong, Cuong AU - Kidder, Joshua B AU - Jacobson, Erik R AU - Otto, Michael AU - Proctor, Richard A AU - Somerville, Greg A AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, Montana 59840. Departments of Medical Microbiology and Immunology. Medicine, University of Wisconsin Medical School, 1300 University Ave., Madison, Wisconsin 53706. University of Nebraska-Lincoln, Department of Biochemistry, Beadle Center, University of Nebraska, Lincoln, Nebraska 68588-0664. University of Nebraska-Lincoln, Department of Veterinary and Biomedical Sciences, 120 VBS Fair St., Lincoln, Nebraska 68583-0905 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 2967 EP - 2973 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 9 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology KW - Growth rate KW - Adhesins KW - Glucose KW - Intracellular levels KW - Stress KW - ATP KW - Polysaccharides KW - Salts KW - Oxygen KW - Heat KW - Environmental changes KW - Biofilms KW - Tricarboxylic acid cycle KW - Environmental conditions KW - Staphylococcus epidermidis KW - Ethanol KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17875150?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Staphylococcus+epidermidis+Polysaccharide+Intercellular+Adhesin+Production+Significantly+Increases+during+Tricarboxylic+Acid+Cycle+Stress&rft.au=Vuong%2C+Cuong%3BKidder%2C+Joshua+B%3BJacobson%2C+Erik+R%3BOtto%2C+Michael%3BProctor%2C+Richard+A%3BSomerville%2C+Greg+A&rft.aulast=Vuong&rft.aufirst=Cuong&rft.date=2005-05-01&rft.volume=187&rft.issue=9&rft.spage=2967&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Growth rate; Adhesins; Intracellular levels; Glucose; ATP; Stress; Polysaccharides; Oxygen; Salts; Heat; Environmental changes; Biofilms; Environmental conditions; Tricarboxylic acid cycle; Ethanol; Staphylococcus epidermidis ER - TY - JOUR T1 - Expression systems for cloned xenobiotic transporters AN - 17874378; 6250369 AB - One challenge of modern biology is to be able to match genes and their encoded proteins with events at the molecular, cellular, tissue, and organism levels, and thus, provide a multi-level understanding of gene function and dysfunction. How well this can be done for xenobiotic transporters depends on a knowledge of the genes expressed in the tissue, the cellular locations of the gene products (do they function for uptake or efflux?), and our ability to match substrates with transporters using information obtained from cloned transporters functioning in heterologous expression systems. Clearly, making a rational choice of expression system to use for the characterization and study of cloned xenobiotic transporters is a critical part of study design. This choice requires well-defined goals, as well as an understanding of the strengths and weaknesses of candidate expression systems. JF - Toxicology and Applied Pharmacology AU - Pritchard, J B AU - Miller, D S AD - Laboratory of Pharmacology and Chemistry, NIH/National Institute of Environmental Health Sciences, 110 Alexander Drive, MD F1-03, Research Triangle Park, NC 27709, USA, pritcha3@niehs.nih.gov Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 256 EP - 262 VL - 204 IS - 3 SN - 0041-008X, 0041-008X KW - Toxicology Abstracts KW - Cloning KW - Xenobiotics KW - X 24240:Miscellaneous UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17874378?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Expression+systems+for+cloned+xenobiotic+transporters&rft.au=Pritchard%2C+J+B%3BMiller%2C+D+S&rft.aulast=Pritchard&rft.aufirst=J&rft.date=2005-05-01&rft.volume=204&rft.issue=3&rft.spage=256&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.11.018 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Xenobiotics; Cloning DO - http://dx.doi.org/10.1016/j.taap.2004.11.018 ER - TY - JOUR T1 - The role of IFN regulatory factor-3 in the cytotoxic activity of NS-9, a polyinosinic-polycytidylic acid/cationic liposome complex, against tumor cells AN - 17873841; 6278439 AB - NS-9 is a complex of polyinosinic-polycytidylic acid and a novel cationic liposome, LIC-101. The complex has strong cytotoxic activity against tumor cells derived from epithelial or fibroblastic cells. We have investigated the mechanism of the cytotoxic activity of NS-9 using knockdown cells in which the expression of proteins of interest was inhibited by RNA interference. NS-9 showed strong cytotoxic activity against knockdown cells with reduced expression of double-stranded RNA-dependent protein kinase, RNase L, or IFN- alpha / beta receptor, but showed no cytotoxic activity against IFN regulatory factor-3 (IRF3) knockdown cells. In IRF3-knockdown cells, NS-9 also did not induce either the DNA fragmentation or the rRNA degradation observed in negative control cells. We conclude that IRF3 plays a crucial role in the cytotoxic activity of NS-9 against tumor cells, whereas RNA-dependent protein kinase, RNase L, or type I IFNs are not important for its activity. JF - Molecular Cancer Therapeutics AU - Uno, Tomonori AU - Hirabayashi, Kazuko AU - Murai, Masatoshi AU - Yano, Junichi AU - Ozato, Keiko AD - Discovery Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan and Laboratory of Molecular Growth and Regulation, The National Institute of Child Health and Human Development, NIH, Bethesda, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 799 EP - 805 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 4 IS - 5 SN - 1535-7163, 1535-7163 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - ^a-Interferon KW - DNA fragmentation KW - rRNA KW - Cytotoxicity KW - Poly (I:C) KW - RNA-mediated interference KW - Protein kinase KW - Liposomes KW - Tumor cells KW - W3 33374:Antitumor agents KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17873841?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Cancer+Therapeutics&rft.atitle=The+role+of+IFN+regulatory+factor-3+in+the+cytotoxic+activity+of+NS-9%2C+a+polyinosinic-polycytidylic+acid%2Fcationic+liposome+complex%2C+against+tumor+cells&rft.au=Uno%2C+Tomonori%3BHirabayashi%2C+Kazuko%3BMurai%2C+Masatoshi%3BYano%2C+Junichi%3BOzato%2C+Keiko&rft.aulast=Uno&rft.aufirst=Tomonori&rft.date=2005-05-01&rft.volume=4&rft.issue=5&rft.spage=799&rft.isbn=&rft.btitle=&rft.title=Molecular+Cancer+Therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cytotoxicity; Tumor cells; Protein kinase; Liposomes; Poly (I:C); rRNA; RNA-mediated interference; ^a-Interferon; DNA fragmentation ER - TY - JOUR T1 - SspA is required for acid resistance in stationary phase by downregulation of H-NS in Escherichia coli AN - 17866552; 6230247 AB - The stringent starvation protein A (SspA) is a RNA polymerase-associated protein and is required for transcriptional activation of bacteriophage P1 late promoters. However, the role of SspA in gene expression in Escherichia coli is essentially unknown. In this work, we show that SspA is essential for cell survival during acid-induced stress. Apparently, SspA inhibits stationary-phase accumulation of H-NS, a global regulator which functions mostly as a repressor, thereby derepressing multiple stress defence systems including those for acid stress and nutrient starvation. Consequently, the gene expression pattern of the H-NS regulon is altered in the sspA mutant, leading to acid-sensitive and hypermotile phenotypes. Thus, our study indicates that SspA is a global regulator, which acts upstream of H-NS, and thereby plays an important role in the stress response of E. coli during stationary phase. In addition, our results indicate that the expression of the H-NS regulon is sensitive to small changes in the cellular level of H-NS, enabling the cell to response rapidly to environment cues. As SspA and H-NS are highly conserved among Gram-negative bacteria, of which many are pathogenic, the global role of SspA in the stress response and pathogenesis is discussed. JF - Molecular Microbiology AU - Hansen, Anne-Marie AU - Qiu, Yu AU - Yeh, Norman AU - Blattner, Frederick R AU - Durfee, Tim AU - Jin, Ding Jun AD - Transcription Control Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, NIH, Bldg. 469, PO Box B, Frederick, MD 21702, USA, djjin@helix.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 719 EP - 734 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 56 IS - 3 SN - 0950-382X, 0950-382X KW - sspA protein KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - Phages KW - Cell survival KW - Nutrients KW - Gene expression KW - stationary phase KW - Promoters KW - Gram-negative bacteria KW - Escherichia coli KW - Starvation KW - Stress KW - RNA KW - Repressors KW - Transcription activation KW - J 02722:Biodegradation, growth, nutrition and leaching KW - N 14080:Other UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17866552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Microbiology&rft.atitle=SspA+is+required+for+acid+resistance+in+stationary+phase+by+downregulation+of+H-NS+in+Escherichia+coli&rft.au=Hansen%2C+Anne-Marie%3BQiu%2C+Yu%3BYeh%2C+Norman%3BBlattner%2C+Frederick+R%3BDurfee%2C+Tim%3BJin%2C+Ding+Jun&rft.aulast=Hansen&rft.aufirst=Anne-Marie&rft.date=2005-05-01&rft.volume=56&rft.issue=3&rft.spage=719&rft.isbn=&rft.btitle=&rft.title=Molecular+Microbiology&rft.issn=0950382X&rft_id=info:doi/10.1111%2Fj.1365-2958.2005.04567.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - SuppNotes - Figures, 9; tables, 1; references, 104. N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Stress; stationary phase; Gene expression; Starvation; Promoters; Transcription activation; Cell survival; Gram-negative bacteria; Repressors; Phages; Nutrients; RNA DO - http://dx.doi.org/10.1111/j.1365-2958.2005.04567.x ER - TY - JOUR T1 - Parent-imposed limits on high-risk adolescent driving: are they stricter with graduated driver licensing? AN - 17813998; 6213940 AB - The purpose of this study was to determine whether parent-imposed limits on 16-year-old high-risk driving are stricter in Maryland (MD), a state with graduated driver licensing (GDL) than in Connecticut (CT), a non-GDL state. In both states, parents and adolescents completed telephone surveys about the restrictions that parents placed on their adolescents' driving at night, with adolescent passengers, and at high speeds. In Maryland, surveys took place 1 month (294 parent-adolescent pairs) and 4 months (292 parent-adolescent pairs) after provisional licensure. In Connecticut, surveys took place the first month (132 pairs) and the third month (108 pairs) after adolescent licensure. The findings indicated that after controlling for demographic characteristics, Maryland parents and adolescents reported stricter parent-imposed limits for adolescent passengers, high-speed roads, weekend night driving, and overall limits. Parents in GDL states appear better able to establish and enforce adolescent driving restrictions when the licensing state stipulates, favors, and supports regulated adolescent driving. JF - Accident Analysis & Prevention AU - Hartos, J L AU - Simons-Morton, B G AU - Beck, KH AU - Leaf, WA AD - Interdisciplinary Health Studies, Minor Barnard 221, Department of Health Behavior & Administration, College of Health & Human Services, UNC Charlotte, 9201 University Blvd, Charlotte, NC 28223-0001, USA, jessica_hartos@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 557 EP - 562 PB - Elsevier Science Ltd., Pergamon, P.O. Box 800 Kidlington Oxford OX5 1DX UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 37 IS - 3 SN - 0001-4575, 0001-4575 KW - graduated driver licensing KW - Health & Safety Science Abstracts; Risk Abstracts KW - USA, Connecticut KW - Motor vehicles KW - Licensing KW - Accidents KW - driving ability KW - State programs KW - prevention KW - Highways KW - USA, Maryland KW - Adolescents KW - Government programs KW - Traffic safety KW - R2 23020:Technological risks KW - H 2000:Transportation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17813998?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Accident+Analysis+%26+Prevention&rft.atitle=Parent-imposed+limits+on+high-risk+adolescent+driving%3A+are+they+stricter+with+graduated+driver+licensing%3F&rft.au=Hartos%2C+J+L%3BSimons-Morton%2C+B+G%3BBeck%2C+KH%3BLeaf%2C+WA&rft.aulast=Hartos&rft.aufirst=J&rft.date=2005-05-01&rft.volume=37&rft.issue=3&rft.spage=557&rft.isbn=&rft.btitle=&rft.title=Accident+Analysis+%26+Prevention&rft.issn=00014575&rft_id=info:doi/10.1016%2Fj.aap.2005.01.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - USA, Connecticut; USA, Maryland; Adolescents; driving ability; Government programs; State programs; Licensing; Accidents; Motor vehicles; Traffic safety; prevention; Highways DO - http://dx.doi.org/10.1016/j.aap.2005.01.008 ER - TY - JOUR T1 - A CD8 super(+) T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity against Chlamydia pneumoniae AN - 17624956; 6275613 AB - An intact T cell compartment and IFN- gamma signaling are required for protective immunity against CHLAMYDIA: In the mouse model of Chlamydia pneumoniae (Cpn) infection, this immunity is critically dependent on CD8 super(+) T cells. Recently we reported that Cpn-infected mice generate an MHC class I-restricted CD8 super(+) Tc1 response against various Cpn Ags, and that CD8 super(+) CTL to multiple epitopes inhibit Cpn growth in vitro. Here, we engineered a DNA minigene encoding seven H-2 super(b)-restricted Cpn CTL epitopes, the universal pan-DR epitope Th epitope, and an endoplasmic reticulum-translocating signal sequence. Immunization of C57BL/6 mice with this construct primed IFN- gamma -producing CD8 super(+) CTL against all seven CTL epitopes. CD8 super(+) T cell lines generated to minigene-encoded CTL epitopes secreted IFN- gamma and TNF- alpha and exhibited CTL activity upon recognition of Cpn-infected macrophages. Following intranasal challenge with Cpn, a 3.6 log reduction in mean lung bacterial numbers compared with control animals was obtained. Using a 20-fold increase in the Cpn challenging dose, minigene-vaccinated mice had a 60-fold reduction in lung bacterial loads, compared with controls. Immunization and challenge studies with beta sub(2)-microglobulin super(-/-) mice indicated that the reduction of lung Cpn burdens was mediated by the MHC class I-dependent CD8 super(+) T cells to minigene-included Cpn CTL epitopes, rather than by pan-DR epitope-specific CD4 super(+) T cells. This constitutes the first demonstration of significant protection achieved by immunization with a CD8 super(+) T cell epitope-based DNA construct in a bacterial system and provides the basis for the optimal design of multicomponent anti-Cpn vaccines for humans. JF - Journal of Immunology AU - Pinchuk, Irina AU - Starcher, Barry C AU - Livingston, Brian AU - Tvninnereim, Amy AU - Wu, Shiping AU - Appella, Ettore AU - Sidney, John AU - Sette, Alessandro AU - Wizel, Benjamin AD - Center for Pulmonary and Infectious Disease Control, Departments of Microbiology and Immunology and Biochemistry, University of Texas Health Center, Tyler, TX 75708. Epimmune and La Jolla Institute for Allergy and Immunology, San Diego, CA 92121. National Cancer Institute, Bethesda, MD 20892 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5729 EP - 5739 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - mice KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - J 02834:Vaccination and immunization KW - F 06100:Vaccines - active immunity KW - J 02845:Ear, nose and respiratory tract UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17624956?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=A+CD8+super%28%2B%29+T+Cell+Heptaepitope+Minigene+Vaccine+Induces+Protective+Immunity+against+Chlamydia+pneumoniae&rft.au=Pinchuk%2C+Irina%3BStarcher%2C+Barry+C%3BLivingston%2C+Brian%3BTvninnereim%2C+Amy%3BWu%2C+Shiping%3BAppella%2C+Ettore%3BSidney%2C+John%3BSette%2C+Alessandro%3BWizel%2C+Benjamin&rft.aulast=Pinchuk&rft.aufirst=Irina&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5729&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Dendritic Cells Endocytose Bacillus anthracis Spores: Implications for Anthrax Pathogenesis AN - 17624278; 6275591 AB - Phagocytosis of inhaled Bacillus anthracis spores and subsequent trafficking to lymph nodes are decisive events in the progression of inhalational anthrax because they initiate germination and dissemination of spores. Found in high frequency throughout the respiratory track, dendritic cells (DCs) routinely take up foreign particles and migrate to lymph nodes. However, the participation of DCs in phagocytosis and dissemination of spores has not been investigated previously. We found that human DCs readily engulfed fully pathogenic Ames and attenuated B. anthracis spores predominately by coiling phagocytosis. Spores provoked a loss of tissue-retaining chemokine receptors (CCR2, CCR5) with a concurrent increase in lymph node homing receptors (CCR7, CD11c) on the membrane of DCs. After spore infection, immature DCs displayed a mature phenotype (CD83 super(bright), HLA-DR super(bright), CD80 super(bright), CD86 super(bright), CD40 super(bright)) and enhanced costimulatory activity. Surprisingly, spores activated the MAPK cascade (ERK, p38) within 30 min and stimulated expression of several inflammatory response genes by 2 h. MAPK signaling was extinguished by 6 h infection, and there was a dramatic reduction of secreted TNF- alpha , IL-6, and IL-8 in the absence of DC death. This corresponded temporally with enzymatic cleavage of proximal MAPK signaling proteins (MEK-1, MEK-3, and MAP kinase kinase-4) and may indicate activity of anthrax lethal toxin. Taken together, these results suggest that B. anthracis may exploit DCs to facilitate infection. JF - Journal of Immunology AU - Brittingham, Katherine C AU - Ruthel, Gordon AU - Panchal, Rekha G AU - Fuller, Claudette L AU - Ribot, Wilson J AU - Hoover, Timothy A AU - Young, Howard A AU - Anderson, Arthur O AU - Bavari, Sina AD - U.S. Army Medical Research Institute of Infectious Diseases, and. Developmental Therapeutics Program and. Laboratory of Experimental Immunology, Centre for Cancer Research, National Cancer Institute, Frederick, MD 21702 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5545 EP - 5552 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - J 02833:Immune response and immune mechanisms KW - F 06106:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17624278?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Dendritic+Cells+Endocytose+Bacillus+anthracis+Spores%3A+Implications+for+Anthrax+Pathogenesis&rft.au=Brittingham%2C+Katherine+C%3BRuthel%2C+Gordon%3BPanchal%2C+Rekha+G%3BFuller%2C+Claudette+L%3BRibot%2C+Wilson+J%3BHoover%2C+Timothy+A%3BYoung%2C+Howard+A%3BAnderson%2C+Arthur+O%3BBavari%2C+Sina&rft.aulast=Brittingham&rft.aufirst=Katherine&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5545&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Measurement of Vasoactive Intestinal Peptide using a Competitive Fluorescent Microsphere Immunoassay or ELISA in human blood samples AN - 17623683; 6396017 AB - The concentration of Vasoactive Intestinal Peptide (VIP) as measured by recycling immunoaffinity chromatography (RIC) has been reported to be elevated in the blood of patients with autism as compared with normal subjects. In this study, we have developed a ''Competitive Fluorescent Microsphere Immunoassay'' (cFMI) in which VIP competes with biotinylated VIP in binding to polyclonal antibodies on microspheres. The results were obtained using the Luminex super(1) super(0) super(0) system. We measured VIP in serum, plasma, and material eluted from dried blood spots on filter paper with both the cFMI and an ELISA procedure. We found that a purification procedure was necessary for obtaining useful results from plasma and serum, however, a preincubation step was required with the blood eluates. This newly developed cFMI was more sensitive (2.5 vs. 20.0 pg/ml), and more reproducible than the ELISA. To get accurate measurements of VIP in eluted material high sensitivity is especially important. Thus, the cFMI using the Luminex system has definite advantages over a conventional ELISA including the possibility that samples can be assayed at higher dilutions. We have determined that the VIP concentrations of serum, plasma, and dried blood spot eluate specimens as measured with the cFMI assay system were similar to those measured with ELISA. Thus, the new cFMI using Luminex system may be useful for detection of VIP in human blood samples. JF - Journal of Immunological Methods AU - Song, E Y AU - VanDunk, C AU - Kuddo, T AU - Nelson, P G AD - National Institute of Child Health and Human Development, National Institutes of Health, Building 31 Room 2 A 25, Bethesda, MD 20892-4480, USA, nelsonp@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 63 EP - 73 VL - 300 IS - 1-2 SN - 0022-1759, 0022-1759 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - W3 33240:Immunology KW - F 06704:Immunoassays KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17623683?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunological+Methods&rft.atitle=Measurement+of+Vasoactive+Intestinal+Peptide+using+a+Competitive+Fluorescent+Microsphere+Immunoassay+or+ELISA+in+human+blood+samples&rft.au=Song%2C+E+Y%3BVanDunk%2C+C%3BKuddo%2C+T%3BNelson%2C+P+G&rft.aulast=Song&rft.aufirst=E&rft.date=2005-05-01&rft.volume=300&rft.issue=1-2&rft.spage=63&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunological+Methods&rft.issn=00221759&rft_id=info:doi/10.1016%2Fj.jim.2005.02.009 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1016/j.jim.2005.02.009 ER - TY - JOUR T1 - Characteristics Of A Human Prostate Stromal Cell Line Related To Its Use In A Stromal-Epithelial Coculture Model For The Study Of Cancer Chemoprevention AN - 17575666; 6458232 AB - An immortalized human prostate stromal cell line (PS30) was previously established using recombinant retrovirus encoding human papillomavirus 16 gene products. In this study, we further characterize this stromal cell line for its potential use in a stromal-epithelial coculture model for prostate cancer prevention. Using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and immunocytochemistry, we examined expression of androgen receptor (AR), vitamin D receptor (VDR), prostate-specific antigen (PSA), transforming growth factor- beta (TGF- beta ), and insulin-like growth factors (IGF) families and their receptors, metalloproteinases (MMP) MMP-2 and MMP-9, as well as the cells' ability to respond to the synthetic androgen R1881. The PS30 stromal cells do not express PSA, confirming their stromal origin. They are positive for both AR messenger ribonucleic acid (mRNA) and protein; however, they do not respond to growth stimulation by the synthetic androgen R1881. The PS30 cells express mRNA for VDR, TGF- beta s, IGFs and their receptors, as well as the MMPs. Moreover, they produce significant amounts of TGF- beta 1, TGF- beta 2, IGFBP-3, and MMP-2 proteins. Our observations confirm the use of PS30 for the study of stromal-epithelial interactions in the modulation of prostate carcinogenesis. JF - In Vitro Cellular & Developmental Biology - Animal AU - Diaw, L AU - Roth, M AU - Schwinn, DA AU - d'Alelio, ME AU - Green, L J AU - Tangrea, JA AD - SAIC-Frederick Inc., National Cancer Institute/Advanced Technology Center, Bethesda, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 142 EP - 148 PB - Society for In Vitro Biology VL - 41 IS - 5 SN - 1071-2690, 1071-2690 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - stromal cells KW - prostate-specific antigen KW - Transforming growth factor-^b1 KW - Androgen receptors KW - Metalloproteinase KW - Prostate cancer KW - Human papillomavirus 16 KW - Insulin-like growth factors KW - Vitamin D receptors KW - Transforming growth factor-^b KW - Gelatinase A KW - Gelatinase B KW - Insulin-like growth factor-binding protein 3 KW - W 30965:Miscellaneous, Reviews KW - W3 33220:Cell culture UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17575666?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=In+Vitro+Cellular+%26+Developmental+Biology+-+Animal&rft.atitle=Characteristics+Of+A+Human+Prostate+Stromal+Cell+Line+Related+To+Its+Use+In+A+Stromal-Epithelial+Coculture+Model+For+The+Study+Of+Cancer+Chemoprevention&rft.au=Diaw%2C+L%3BRoth%2C+M%3BSchwinn%2C+DA%3Bd%27Alelio%2C+ME%3BGreen%2C+L+J%3BTangrea%2C+JA&rft.aulast=Diaw&rft.aufirst=L&rft.date=2005-05-01&rft.volume=41&rft.issue=5&rft.spage=142&rft.isbn=&rft.btitle=&rft.title=In+Vitro+Cellular+%26+Developmental+Biology+-+Animal&rft.issn=10712690&rft_id=info:doi/10.1290%2F0412079.1 L2 - http://journals.allenpress.com/jrnlserv/?request=get-abstract&issn=1071-2690&volume=41&issue=5&page=142 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Human papillomavirus 16; Transforming growth factor-^b; stromal cells; Insulin-like growth factors; Vitamin D receptors; Gelatinase A; Transforming growth factor-^b1; Gelatinase B; prostate-specific antigen; Insulin-like growth factor-binding protein 3; Metalloproteinase; Androgen receptors; Prostate cancer DO - http://dx.doi.org/10.1290/0412079.1 ER - TY - JOUR T1 - A web-based tool for principal component and significance analysis of microarray data AN - 17565788; 6270208 AB - SUMMARY: We have developed a program for microarray data analysis, which features the false discovery rate for testing statistical significance and the principal component analysis using the singular value decomposition method for detecting the global trends of gene-expression patterns. Additional features include analysis of variance with multiple methods for error variance adjustment, correction of cross-channel correlation for two-color microarrays, identification of genes specific to each cluster of tissue samples, biplot of tissues and corresponding tissue-specific genes, clustering of genes that are correlated with each principal component (PC), three-dimensional graphics based on virtual reality modeling language and sharing of PC between different experiments. The software also supports parameter adjustment, gene search and graphical output of results. The software is implemented as a web tool and thus the speed of analysis does not depend on the power of a client computer. AVAILABILITY: The tool can be used on-line or downloaded at http://lgsun.grc.nia.nih.gov/ANOVA/ JF - Bioinformatics AU - Sharov, Alexei A AU - Dudekula, Dawood B AU - Ko, Minoru SH AD - Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health Baltimore, MD 21224, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2548 EP - 2549 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 10 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Computer programs KW - software KW - Statistics KW - Data processing KW - Principal components analysis KW - Computer graphics KW - Language KW - Bioinformatics KW - Computer applications KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17565788?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=A+web-based+tool+for+principal+component+and+significance+analysis+of+microarray+data&rft.au=Sharov%2C+Alexei+A%3BDudekula%2C+Dawood+B%3BKo%2C+Minoru+SH&rft.aulast=Sharov&rft.aufirst=Alexei&rft.date=2005-05-01&rft.volume=21&rft.issue=10&rft.spage=2548&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Computer programs; Data processing; software; Statistics; Bioinformatics; Language; Principal components analysis; Computer applications; Gene expression; Computer graphics ER - TY - JOUR T1 - Characterizing dye bias in microarray experiments AN - 17565093; 6270184 AB - MOTIVATION: Spot intensity serves as a proxy for gene expression in dual-label microarray experiments. Dye bias is defined as an intensity difference between samples labeled with different dyes attributable to the dyes instead of the gene expression in the samples. Dye bias that is not removed by array normalization can introduce bias into comparisons between samples of interest. But if the bias is consistent across samples for the same gene, it can be corrected by proper experimental design and analysis. If the dye bias is not consistent across samples for the same gene, but is different for different samples, then removing the bias becomes more problematic, perhaps indicating a technical limitation to the ability of fluorescent signals to accurately represent gene expression. Thus, it is important to characterize dye bias to determine: (1) whether it will be removed for all genes by array normalization, (2) whether it will not be removed by normalization but can be removed by proper experimental design and analysis and (3) whether dye bias correction is more problematic than either of these and is not easily removable. RESULTS: We analyzed two large (each >27 arrays) tissue culture experiments with extensive dye swap arrays to better characterize dye bias. Indirect, amino-allyl labeling was used in both experiments. We found that post-normalization dye bias that is consistent across samples does appear to exist for many genes, and that controlling and correcting for this type of dye bias in design and analysis is advisable. The extent of this type of dye bias remained unchanged under a wide range of normalization methods (median-centering, various loess normalizations) and statistical analysis techniques (parametric, rank based, permutation based, etc.). We also found dye bias related to the individual samples for a much smaller subset of genes. But these sample-specific dye biases appeared to have minimal impact on estimated gene-expression differences between the cell lines. AVAILABILITY: Supplementary information: http://linus.nci.nih.gov/~brb/TechReport.htm JF - Bioinformatics AU - Dobbin, K K AU - Kawasaki, E S AU - Petersen, D W AU - Simon, R M AD - Biometric Research Branch, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA. Advanced Technology Center, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2430 EP - 2437 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 10 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Dyes KW - Statistical analysis KW - Bioinformatics KW - Tissue culture KW - DNA microarrays KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17565093?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Characterizing+dye+bias+in+microarray+experiments&rft.au=Dobbin%2C+K+K%3BKawasaki%2C+E+S%3BPetersen%2C+D+W%3BSimon%2C+R+M&rft.aulast=Dobbin&rft.aufirst=K&rft.date=2005-05-01&rft.volume=21&rft.issue=10&rft.spage=2430&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Gene expression; Dyes; Tissue culture; Statistical analysis; Bioinformatics; DNA microarrays ER - TY - JOUR T1 - Synthesis and Characterization of Lipooligosaccharide-Based Conjugate Vaccines for Serotype B Moraxella catarrhalis AN - 17543725; 6266374 AB - Moraxella catarrhalis is an important cause of otitis media in children and respiratory tract infections in the elderly. Lipooligosaccharide (LOS) is a major surface antigen of the bacterium that elicits bactericidal antibodies. Serological studies show that three major LOS types (A, B, and C) have been identified among clinical isolates. Our previous studies demonstrated that the type A LOS-based conjugates were immunogenic in animals. In this study, LOS from type B strain 26397 was detoxified and conjugated to tetanus toxoid (TT) or a cross-reactive mutant (CRM) of diphtheria toxin to form detoxified LOS (dLOS)-TT and dLOS-CRM, respectively, as vaccine candidates. The molar ratios of dLOS to TT and CRM in the conjugates were 43:1 and 19:1, respectively, while both weight ratios were around 0.9. The antigenicity of the conjugates was similar to that of the LOS, as determined by enzyme-linked immunosorbent assay using a rabbit antiserum to strain 26397. Subcutaneous immunization with each conjugate elicited a 180- to 230-fold rise of serum anti-LOS immunoglobulin G in mice and >2,000-fold rise in rabbits. In addition, both mouse and rabbit antisera showed elevated complement-mediated bactericidal activity against the homologous strain, and a representative rabbit antiserum showed bactericidal activity against nine of twelve clinical isolates studied. The bactericidal activity of the rabbit antiserum can be fully inhibited by the type B LOS but not the A or C LOS. These results indicate that the type B LOS-based conjugates can be used as vaccine components for further investigation. JF - Infection and Immunity AU - Yu, Shengqing AU - Gu, Xin-Xing AD - Vaccine Research Facility, National Institute on Deafness and Other Communication Disorders, Rockville, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2790 EP - 2796 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 5 SN - 0019-9567, 0019-9567 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - Antigenicity KW - Toxoids KW - Tetanus KW - Geriatrics KW - Clinical isolates KW - Enzyme-linked immunosorbent assay KW - Moraxella catarrhalis KW - Immunization KW - Lipooligosaccharides KW - Otitis media KW - Immunoglobulin G KW - Vaccines KW - Serotypes KW - Complement KW - Infection KW - Bactericidal activity KW - Children KW - Diphtheria toxin KW - Respiratory tract diseases KW - Antisera KW - surface antigens KW - W3 33365:Vaccines (other) KW - J 02834:Vaccination and immunization KW - F 06100:Vaccines - active immunity KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17543725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Synthesis+and+Characterization+of+Lipooligosaccharide-Based+Conjugate+Vaccines+for+Serotype+B+Moraxella+catarrhalis&rft.au=Yu%2C+Shengqing%3BGu%2C+Xin-Xing&rft.aulast=Yu&rft.aufirst=Shengqing&rft.date=2005-05-01&rft.volume=73&rft.issue=5&rft.spage=2790&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Moraxella catarrhalis; Vaccines; Bactericidal activity; Clinical isolates; Geriatrics; Lipooligosaccharides; Diphtheria toxin; Complement; Children; surface antigens; Respiratory tract diseases; Otitis media; Serotypes; Enzyme-linked immunosorbent assay; Antisera; Tetanus; Immunoglobulin G; Immunization; Antigenicity; Toxoids; Infection ER - TY - JOUR T1 - Plasmid Partition System of the P1par Family from the pWR100 Virulence Plasmid of Shigella flexneri AN - 17542511; 6266463 AB - P1par family members promote the active segregation of a variety of plasmids and plasmid prophages in gram-negative bacteria. Each has genes for ParA and ParB proteins, followed by a parS partition site. The large virulence plasmid pWR100 of Shigella flexneri contains a new P1par family member: pWR100par. Although typical parA and parB genes are present, the putative pWR100parS site is atypical in sequence and organization. However, pWR100parS promoted accurate plasmid partition in Escherichia coli when the pWR100 Par proteins were supplied. Unique BoxB hexamer motifs within parS define species specificities among previously described family members. Although substantially different from P1parS from the P1 plasmid prophage of E. coli, pWR100parS has the same BoxB sequence. As predicted, the species specificity of the two types proved identical. They also shared partition-mediated incompatibility, consistent with the proposed mechanistic link between incompatibility and species specificity. Among several informative sequence differences between pWR100parS and P1parS is the presence of a 21-bp insert at the center of the pWR100parS site. Deletion of this insert left much of the parS activity intact. Tolerance of central inserts with integral numbers of helical DNA turns reflects the critical topology of these sites, which are bent by binding the host IHF protein. JF - Journal of Bacteriology AU - Sergueev, Kirill AU - Dabrazhynetskaya, Alena AU - Austin, Stuart AD - Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute, NCI-FCRDC, Frederick, Maryland 21702-1201 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3369 EP - 3373 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 10 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - hexamers KW - Deletion KW - IHF protein KW - ParB protein KW - Plasmids KW - Prophages KW - Virulence KW - Gram-negative bacteria KW - Shigella flexneri KW - DNA KW - Escherichia coli KW - J 02760:Plasmids KW - G 07203:Plasmids UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17542511?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Plasmid+Partition+System+of+the+P1par+Family+from+the+pWR100+Virulence+Plasmid+of+Shigella+flexneri&rft.au=Sergueev%2C+Kirill%3BDabrazhynetskaya%2C+Alena%3BAustin%2C+Stuart&rft.aulast=Sergueev&rft.aufirst=Kirill&rft.date=2005-05-01&rft.volume=187&rft.issue=10&rft.spage=3369&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Shigella flexneri; Plasmids; Virulence; Prophages; Deletion; hexamers; Gram-negative bacteria; IHF protein; ParB protein; DNA ER - TY - JOUR T1 - Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences AN - 17541084; 6266402 AB - Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated amino acid sequences that were absent in the Lyme disease spirochete Borrelia burgdorferi. The predicted amino acid sequences of these genes (BH0209, BH0512, and BH0553) have hydrophobic N termini, indicating that these proteins may be secreted. B. hermsii transcribed the three ORFs in vitro, and the BH0512- and BH0553-encoded proteins (PBH-512 and PBH-553) were produced in vitro and in experimentally infected mice. PBH-512 and PBH-553 were on the spirochete's outer surface, and antiserum to these proteins reduced the adherence of B. hermsii to red blood cells. PCR analyses of 28 isolates of B. hermsii and 8 isolates of B. turicatae demonstrated polymorphism in each gene correlated with the number of repeats. Serum samples from relapsing fever patients reacted with recombinant PBH-512 and PBH-553, suggesting that these proteins are produced during human infection. These polymorphic proteins may be involved in the pathogenicity of these relapsing fever spirochetes and provide a mechanism for antigenic heterogeneity within their populations. JF - Infection and Immunity AU - Guyard, Cyril AU - Chester, Earl M AU - Raffel, Sandra J AU - Schrumpf, Merry E AU - Policastro, Paul F AU - Porcella, Stephen F AU - Leong, John M AU - Schwan, Tom G AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana. Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, Massachussetts Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3025 EP - 3037 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 5 SN - 0019-9567, 0019-9567 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Genomes KW - Borrelia hermsii KW - Gene polymorphism KW - Nucleotide sequence KW - Relapsing fever KW - Erythrocytes KW - Hydrophobicity KW - Infection KW - Chromosomes KW - Pathogenicity KW - Polymerase chain reaction KW - Repeated DNA sequences KW - Lyme disease KW - Borrelia burgdorferi KW - Borrelia turicatae KW - Spirochetes KW - Open reading frames KW - G 07320:Bacterial genetics KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17541084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Relapsing+Fever+Spirochetes+Contain+Chromosomal+Genes+with+Unique+Direct+Tandemly+Repeated+Sequences&rft.au=Guyard%2C+Cyril%3BChester%2C+Earl+M%3BRaffel%2C+Sandra+J%3BSchrumpf%2C+Merry+E%3BPolicastro%2C+Paul+F%3BPorcella%2C+Stephen+F%3BLeong%2C+John+M%3BSchwan%2C+Tom+G&rft.aulast=Guyard&rft.aufirst=Cyril&rft.date=2005-05-01&rft.volume=73&rft.issue=5&rft.spage=3025&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Spirochetes; Borrelia burgdorferi; Borrelia turicatae; Borrelia hermsii; Relapsing fever; Nucleotide sequence; Hydrophobicity; Lyme disease; Gene polymorphism; Erythrocytes; Chromosomes; Genomes; Infection; Open reading frames; Pathogenicity; Polymerase chain reaction; Repeated DNA sequences ER - TY - JOUR T1 - Recombinant, Live-Attenuated Tetravalent Dengue Virus Vaccine Formulations Induce a Balanced, Broad, and Protective Neutralizing Antibody Response against Each of the Four Serotypes in Rhesus Monkeys AN - 17492585; 6267490 AB - Three tetravalent vaccine (TV) formulations of previously described monovalent dengue (DEN) virus vaccine candidates were compared to a tetravalent formulation of wild-type DEN viruses (T-wt) for replication in SCID mice transplanted with human liver cells (SCID-HuH-7) or for replication and immunogenicity in rhesus monkeys. TV-1 consists of recombinant DEN1, -2, -3, and -4, each with a 30-nucleotide deletion in the 3' untranslated region ( Delta 30). TV-2 consists of rDEN1 Delta 30, rDEN4 Delta 30, and two antigenic chimeric viruses, rDEN2/4 Delta 30 and rDEN3/4 Delta 30, both also bearing the Delta 30 mutation. TV-3 consists of rDEN1 Delta 30, rDEN2 Delta 30, rDEN4 Delta 30, and a 10-fold higher dose of rDEN3/4 Delta 30. TV-1 and TV-2 were attenuated in SCID-HuH-7 mice with minimal interference in replication among the virus components. TV-1, -2, and -3 were attenuated in rhesus monkeys as measured by duration and peak of viremia. Each monkey immunized with TV-1 and TV-3 seroconverted to the four DEN components by day 28 with neutralization titers ranging from 1:52 to 1:273 and 1:59 to 1:144 for TV-1 and TV-3, respectively. TV-2 induced low antibody titers to DEN2 and DEN3, but a booster immunization after 4 months increased the neutralizing antibody titers to greater than 1:100 against each serotype and elicited broad neutralizing activity against 19 of 20 DEN subtypes. A single dose of TV-2 induced protection against wild-type DEN1, DEN3, and DEN4 challenge, but not DEN2. However, two doses of TV-2 or TV-3 induced protection against DEN2 challenge. Two tetravalent formulations, TV-2 and TV-3, possess properties of a successful DEN vaccine and can be considered for evaluation in clinical trials. JF - Journal of Virology AU - Blaney, Joseph E, Jr AU - Matro, Jennifer M AU - Murphy, Brian R AU - Whitehead, Stephen S AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5516 EP - 5528 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 9 SN - 0022-538X, 0022-538X KW - Rhesus monkey KW - Rhesus macaque KW - Biotechnology and Bioengineering Abstracts; Immunology Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts KW - Dengue virus KW - Serotypes KW - Hepatocytes KW - Clinical trials KW - Liver transplantation KW - Gene deletion KW - Allografts KW - Macaca mulatta KW - Deletion KW - Replication KW - Antibody response KW - Immunization KW - Immunogenicity KW - Liver KW - Viremia KW - Vaccines KW - Mutation KW - W3 33365:Vaccines (other) KW - V 22097:Immunization: Vaccines & vaccination: Human KW - F 06100:Vaccines - active immunity KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17492585?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Recombinant%2C+Live-Attenuated+Tetravalent+Dengue+Virus+Vaccine+Formulations+Induce+a+Balanced%2C+Broad%2C+and+Protective+Neutralizing+Antibody+Response+against+Each+of+the+Four+Serotypes+in+Rhesus+Monkeys&rft.au=Blaney%2C+Joseph+E%2C+Jr%3BMatro%2C+Jennifer+M%3BMurphy%2C+Brian+R%3BWhitehead%2C+Stephen+S&rft.aulast=Blaney&rft.aufirst=Joseph&rft.date=2005-05-01&rft.volume=79&rft.issue=9&rft.spage=5516&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Macaca mulatta; Dengue virus; Vaccines; Replication; Serotypes; Gene deletion; Immunogenicity; Liver transplantation; Viremia; Clinical trials; Liver; Allografts; Hepatocytes; Antibody response; Deletion; Immunization; Mutation ER - TY - JOUR T1 - Cancer Risk Prediction Models: A Workshop on Development, Evaluation, and Application AN - 17491940; 6276440 AB - Cancer researchers, clinicians, and the public are increasingly interested in statistical models designed to predict the occurrence of cancer. As the number and sophistication of cancer risk prediction models have grown, so too has interest in ensuring that they are appropriately applied, correctly developed, and rigorously evaluated. On May 20-21, 2004, the National Cancer Institute sponsored a workshop in which experts identified strengths and limitations of cancer and genetic susceptibility prediction models that were currently in use and under development and explored methodologic issues related to their development, evaluation, and validation. Participants also identified research priorities and resources in the areas of 1) revising existing breast cancer risk assessment models and developing new models, 2) encouraging the development of new risk models, 3) obtaining data to develop more accurate risk models, 4) supporting validation mechanisms and resources, 5) strengthening model development efforts and encouraging coordination, and 6) promoting effective cancer risk communication and decision-making. JF - Journal of the National Cancer Institute AU - Freedman, Andrew N AU - Seminara, Daniela AU - Gail, Mitchell H AU - Hartge, Patricia AU - Colditz, Graham A AU - Ballard-Barbash, Rachel AU - Pfeiffer, Ruth M AD - Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, MD Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 715 EP - 723 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 97 IS - 10 SN - 0027-8874, 0027-8874 KW - Risk Abstracts KW - Prediction KW - Mathematical models KW - Breast cancer KW - Cancer KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17491940?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Cancer+Risk+Prediction+Models%3A+A+Workshop+on+Development%2C+Evaluation%2C+and+Application&rft.au=Freedman%2C+Andrew+N%3BSeminara%2C+Daniela%3BGail%2C+Mitchell+H%3BHartge%2C+Patricia%3BColditz%2C+Graham+A%3BBallard-Barbash%2C+Rachel%3BPfeiffer%2C+Ruth+M&rft.aulast=Freedman&rft.aufirst=Andrew&rft.date=2005-05-01&rft.volume=97&rft.issue=10&rft.spage=715&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; Prediction; Mathematical models; Breast cancer ER - TY - JOUR T1 - Bactericidal Activity of First-Choice Antibiotics against Gamma Interferon-Induced Persistent Infection of Human Epithelial Cells by Chlamydia trachomatis AN - 17491853; 6276893 AB - Chlamydia trachomatis is responsible for clinically important chronic inflammatory diseases of humans, including trachoma and pelvic inflammatory disease. Persistent infection of mucosal sites may contribute to the development of these chronic inflammatory diseases. Standard clinical therapy results in satisfactory cure rates of acute infections; however, chronic infection associated with persistence has been suggested to be less responsive to antibiotic therapy. We report the efficiency of two first-line chlamydial antibiotics, azithromycin and doxycycline, under conditions of eradication of C. trachomatis persistent infection using the in vitro model of gamma interferon (IFN- gamma )-mediated persistence and reactivation from persistence. Doxycycline was superior in eradicating acute (minimal bactericidal concentration [MBC] sub(100) = 2.5 to 5.0 mu g/ml) compared to persistent (MBC sub(100) = 10 to 50 mu g/ml) infection. In contrast, azithromycin was significantly more effective in eradicating persistent infection (MBC sub(100) = 2.5 to 5.0 mu g/ml) than acute infection (MBC sub(100) = 10 to 50 mu g/ml). The superior bactericidal effect of azithromycin against persistent infection was found to correlate with the enhanced uptake of the drug by IFN- gamma -treated infected epithelial cells. Based on these findings, we hypothesize that azithromycin should be a particularly efficacious anti-infective agent for the eradication of IFN- gamma -induced chlamydial persistent infection in vivo. JF - Antimicrobial Agents & Chemotherapy AU - Reveneau, Nathalie AU - Crane, Deborah D AU - Fischer, Elizabeth AU - Caldwell, Harlan D AD - Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1787 EP - 1793 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 49 IS - 5 SN - 0066-4804, 0066-4804 KW - Microbiology Abstracts B: Bacteriology KW - gamma -Interferon KW - Epithelial cells KW - Chlamydia trachomatis KW - Antibiotics KW - Persistent infection KW - Trachoma KW - Antimicrobial agents KW - Inflammatory diseases KW - Azithromycin KW - Chronic infection KW - Pelvic inflammatory disease KW - Bactericidal activity KW - Doxycycline KW - J 02849:Sexually-transmitted diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17491853?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+Agents+%26+Chemotherapy&rft.atitle=Bactericidal+Activity+of+First-Choice+Antibiotics+against+Gamma+Interferon-Induced+Persistent+Infection+of+Human+Epithelial+Cells+by+Chlamydia+trachomatis&rft.au=Reveneau%2C+Nathalie%3BCrane%2C+Deborah+D%3BFischer%2C+Elizabeth%3BCaldwell%2C+Harlan+D&rft.aulast=Reveneau&rft.aufirst=Nathalie&rft.date=2005-05-01&rft.volume=49&rft.issue=5&rft.spage=1787&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+Agents+%26+Chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Epithelial cells; gamma -Interferon; Inflammatory diseases; Azithromycin; Chronic infection; Pelvic inflammatory disease; Antibiotics; Bactericidal activity; Doxycycline; Persistent infection; Antimicrobial agents; Trachoma; Chlamydia trachomatis ER - TY - JOUR T1 - Solution NMR Structure of the 48-kDa IIA super(Mannose)-HPr Complex of the Escherichia coli Mannose Phosphotransferase System AN - 17490333; 6274971 AB - The solution structure of the 48-kDa IIA super(Man)-HPr complex of the mannose branch of the Escherichia coli phosphotransferase system has been solved by NMR using conjoined rigid body/torsion angle-simulated annealing on the basis of intermolecular nuclear Overhauser enhancement data and residual dipolar couplings. IIA super(Man) is dimeric and has two symmetrically related binding sites per dimer for HPr. A convex surface on HPr, formed primarily by helices 1 and 2, interacts with a deep groove at the interface of the two subunits of IIA super(Man). The interaction surface on IIA super(Man) is predominantly helical, comprising helix 3 from the subunit that bears the active site His-10 and helices 1, 4, and 5 from the other subunit. The total buried accessible surface area at the protein-protein interface is 1450 Aa super(2). The binding sites on the two proteins are complementary in terms of shape and distribution of hydrophobic, hydrophilic, and charged residues. The active site histidines, His-10 of IIA super(Man) and His-15 (italics indicate HPr residues) of HPr, are in close proximity. An associative transition state involving a pentacoordinate phosphoryl group with trigonal bipyramidal geometry bonded to the N- epsilon 2 atom of His-10 and the N- delta 1 atom of His-15 can be readily formed with negligible displacement in the backbone coordinates of the residues immediately adjacent to the active site histidines. Comparing the structures of complexes of HPr with three other structurally unrelated phosphotransferase system proteins, enzymes I, IIA super(glucose), and IIA super(mannitol), reveals a number of common features that provide a molecular basis for understanding how HPr specifically recognizes a wide range of diverse proteins. JF - Journal of Biological Chemistry AU - Williams, David CJr AU - Cai, Mengli AU - Suh, Jeong-Yong AU - Peterkofsky, Alan AU - Clore, GMarius AD - Laboratory of Chemical Physics, NIDDK, and the Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 20775 EP - 20784 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 21 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Mannose KW - Histidine KW - Surface area KW - Escherichia coli KW - N.M.R. KW - Hydrophobicity KW - phosphotransferase KW - J 02728:Enzymes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17490333?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Solution+NMR+Structure+of+the+48-kDa+IIA+super%28Mannose%29-HPr+Complex+of+the+Escherichia+coli+Mannose+Phosphotransferase+System&rft.au=Williams%2C+David+CJr%3BCai%2C+Mengli%3BSuh%2C+Jeong-Yong%3BPeterkofsky%2C+Alan%3BClore%2C+GMarius&rft.aulast=Williams&rft.aufirst=David&rft.date=2005-05-01&rft.volume=280&rft.issue=21&rft.spage=20775&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Surface area; Histidine; Mannose; Hydrophobicity; N.M.R.; phosphotransferase; Escherichia coli ER - TY - JOUR T1 - Selective depletion strategies in allogeneic stem cell transplantation AN - 17475706; 6654778 AB - Despite improved prophylaxis and treatment, GvHD remains a major limitation to optimal allogeneic stem cell transplantation. Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while useful donor immune function is preserved. The elimination of alloreactive and thereby GvHD-mediating T cells has been shown to be feasible in both pre-clinical and more recently clinical studies. However, SD techniques and the translational research needed for clinical application are still under development. Here we summarize and discuss the following aspects of the SD approach: selection of an appropriate allogeneic stimulator; the responder population; the alloresponse; methods for removal of alloreacting T cells; product testing; clinical considerations. Our review highlights the diversity of possible approaches and the need to develop different techniques for specific clinical applications. JF - Cytotherapy AU - Mielke, S AU - Solomon AU - Barrett, A J AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, National Heart Lung and Blood Institute, National Institutes of Health (NIH), Bethesda, Maryland, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 109 EP - 115 VL - 7 IS - 2 SN - 1465-3249, 1465-3249 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - stem cell transplantation KW - Reviews KW - Prophylaxis KW - Lymphocytes T KW - Graft-versus-host reaction KW - W3 33170:Cellular based KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17475706?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cytotherapy&rft.atitle=Selective+depletion+strategies+in+allogeneic+stem+cell+transplantation&rft.au=Mielke%2C+S%3BSolomon%3BBarrett%2C+A+J&rft.aulast=Mielke&rft.aufirst=S&rft.date=2005-05-01&rft.volume=7&rft.issue=2&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=Cytotherapy&rft.issn=14653249&rft_id=info:doi/10.1080%2F14653240510018172 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Graft-versus-host reaction; Reviews; Lymphocytes T; Prophylaxis; stem cell transplantation DO - http://dx.doi.org/10.1080/14653240510018172 ER - TY - JOUR T1 - IQ and Blood Lead from 2 to 7 Years of Age: Are the Effects in Older Children the Residual of High Blood Lead Concentrations in 2-Year-Olds? AN - 17426486; 6566818 AB - Increases in peak blood lead concentrations, which occur at 18-30 months of age in the United States, are thought to result in lower IQ scores at 4-6 years of age, when IQ becomes stable and measurable. Data from a prospective study conducted in Boston suggested that blood lead concentrations at 2 years of age were more predictive of cognitive deficits in older children than were later blood lead concentrations or blood lead concentrations measured concurrently with IQ. Therefore, cross-sectional associations between blood lead and IQ in school-age children have been widely interpreted as the residual effects of higher blood lead concentrations at an earlier age or the tendency of less intelligent children to ingest more leaded dust or paint chips, rather than as a causal relationship in older children. Here we analyze data from a clinical trial in which children were treated for elevated blood lead concentrations (20-44 mu g/dL) at about 2 years of age and followed until 7 years of age with serial IQ tests and measurements of blood lead. We found that cross-sectional associations increased in strength as the children became older, whereas the relation between baseline blood lead and IQ attenuated. Peak blood lead level thus does not fully account for the observed association in older children between their lower blood lead concentrations and IQ. The effect of concurrent blood level on IQ may therefore be greater than currently believed. JF - Environmental Health Perspectives AU - Chen, A AU - Dietrich, K N AU - Ware, J H AU - Radcliffe, J AU - Rogan, W J AD - Epidemiology Branch, NIEHS, MD A3-05, P.O. Box 12233, Research Triangle Park, NC 27709, USA, rogan@niehs.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 597 EP - 601 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Toxicology Abstracts; Pollution Abstracts; Health & Safety Science Abstracts KW - Age KW - Data processing KW - Environmental health KW - Ingestion KW - Children KW - Clinical trials KW - Lead KW - Dust KW - Blood levels KW - USA, Massachusetts, Boston KW - Cognitive ability KW - Residual effects KW - Paints KW - H 12000:Epidemiology and Public Health KW - P 6000:TOXICOLOGY AND HEALTH KW - X 24163:Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17426486?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=IQ+and+Blood+Lead+from+2+to+7+Years+of+Age%3A+Are+the+Effects+in+Older+Children+the+Residual+of+High+Blood+Lead+Concentrations+in+2-Year-Olds%3F&rft.au=Chen%2C+A%3BDietrich%2C+K+N%3BWare%2C+J+H%3BRadcliffe%2C+J%3BRogan%2C+W+J&rft.aulast=Chen&rft.aufirst=A&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=597&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7625 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Data processing; Cognitive ability; Residual effects; Children; Clinical trials; Dust; Lead; Paints; Blood levels; Age; Environmental health; Ingestion; USA, Massachusetts, Boston DO - http://dx.doi.org/10.1289/ehp.7625 ER - TY - JOUR T1 - Ascorbate Depletion: A Critical Step in Nickel Carcinogenesis? AN - 17425166; 6566815 AB - Nickel compounds are known to cause respiratory cancer in humans and induce tumors in experimental animals. The underlying molecular mechanisms may involve genotoxic effects; however, the data from different research groups are not easy to reconcile. Here, we challenge the common premise that direct genotoxic effects are central to nickel carcinogenesis and probably to that of other metals. Instead, we propose that it is formation of metal complexes with proteins and other molecules that changes cellular homeostasis and provides conditions for selection of cells with transformed phenotype. This is concordant with the major requirement for nickel carcinogenicity, which is prolonged action on the target tissue. If DNA is not the main nickel target, is there another unique molecule that can be attacked with carcinogenic consequences? Our recent observations indicate that ascorbate may be such a molecule. Nickel depletes intracellular ascorbate, which leads to the inhibition of cellular hydroxylases, manifested by the loss of hypoxia-inducible factor (HIF)-1 alpha and -2 alpha hydroxylation and hypoxia-like stress. Proline hydroxylation is crucial for collagen and extracellular matrix assembly as well as for assembly of other protein molecules that have collagen-like domains, including surfactants and complement. Thus, the depletion of ascorbate by chronic exposure to nickel could be deleterious for lung cells and may lead to lung cancer. JF - Environmental Health Perspectives AU - Salnikow, K AU - Kasprzak, K S AD - National Cancer Institute at Frederick, Building 538, Room 205 E, Frederick, MD 21702, USA, salnikow@ncifcrf.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 577 EP - 584 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Toxicology Abstracts KW - Metals KW - Proline KW - Nickel KW - Genotoxicity KW - Ascorbic acid KW - Collagen KW - Hydroxylation KW - Carcinogenesis KW - DNA KW - Hydroxylase KW - Hypoxia-inducible factors KW - Lung cancer KW - X 24165:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17425166?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Ascorbate+Depletion%3A+A+Critical+Step+in+Nickel+Carcinogenesis%3F&rft.au=Salnikow%2C+K%3BKasprzak%2C+K+S&rft.aulast=Salnikow&rft.aufirst=K&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=577&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7605 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Metals; Proline; Genotoxicity; Carcinogenesis; Nickel; DNA; Hydroxylase; Hypoxia-inducible factors; Lung cancer; Hydroxylation; Collagen; Ascorbic acid DO - http://dx.doi.org/10.1289/ehp.7605 ER - TY - JOUR T1 - Endogenous Kappa -Opioid Receptor Systems Regulate Mesoaccumbal Dopamine Dynamics and Vulnerability to Cocaine AN - 17356317; 6277425 AB - Genetic and pharmacological approaches were used to examine Kappa -opioid receptor (KOR-1) regulation of dopamine (DA) dynamics in the nucleus accumbens and vulnerability to cocaine. Microdialysis revealed that basal DA release and DA extraction fraction (E sub(d)), an indirect measure of DA uptake, are enhanced in KOR-1 knock-out mice. Analysis of DA uptake revealed a decreased K sub(m) but unchanged V sub(max) in knock-outs. Knock-out mice exhibited an augmented locomotor response to cocaine, which did not differ from that of wild-types administered a behavioral sensitizing cocaine treatment. The ability of cocaine to increase DA was enhanced in knock-outs, whereas c-fos induction was decreased. Although repeated cocaine administration to wild types produced behavioral sensitization, knock-outs exhibited no additional enhancement of behavior. Administration of the long-acting KOR antagonist nor-binaltorphimine to wild-type mice increased DA dynamics. However, the effects varied with the duration of KOR-1 blockade. Basal DA release was increased whereas E sub(d) was unaltered after 1 h blockade. After 24 h, release and E sub(d) were increased. The behavioral and neurochemical effects of cocaine were enhanced at both time points. These data demonstrate the existence of an endogenous KOR-1 system that tonically inhibits mesoaccumbal DA neurotransmission. Its loss induces neuroadaptations characteristic of "cocaine-sensitized" animals, indicating a critical role of KOR-1 in attenuating responsiveness to cocaine. The increased DA uptake after pharmacological inactivation or gene deletion highlights the plasticity of mesoaccumbal DA neurons and suggests that loss of KOR-1 and the resultant disinhibition of DA neurons trigger short- and long-term DA transporter adaptations that maintain normal DA levels, despite enhanced release. JF - Journal of Neuroscience AU - Chefer, Vladimir I AU - Czyzyk, Traci AU - Bolan, Elizabeth A AU - Moron, Jose AU - Pintar, John E AU - Shippenberg, Toni S AD - Department of Neuroscience and Cell Biology, Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, and Integrative Neuroscience Section, Behavioral Neurosciences Branch, National Institute on Drug Abuse/Intramural Research Program, Baltimore, Maryland 21224 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 5029 EP - 5037 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 20 SN - 0270-6474, 0270-6474 KW - Opioid receptors (type Kappa ) KW - knockout mice KW - CSA Neurosciences Abstracts; Toxicology Abstracts KW - N3 11106:Neurobiology of drug abuse KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17356317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Endogenous+Kappa+-Opioid+Receptor+Systems+Regulate+Mesoaccumbal+Dopamine+Dynamics+and+Vulnerability+to+Cocaine&rft.au=Chefer%2C+Vladimir+I%3BCzyzyk%2C+Traci%3BBolan%2C+Elizabeth+A%3BMoron%2C+Jose%3BPintar%2C+John+E%3BShippenberg%2C+Toni+S&rft.aulast=Chefer&rft.aufirst=Vladimir&rft.date=2005-05-01&rft.volume=25&rft.issue=20&rft.spage=5029&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Pulsed High-Intensity Focused Ultrasound Enhances Systemic Administration of Naked DNA in Squamous Cell Carcinoma Model: Initial Experience AN - 17337134; 6276669 AB - PURPOSE: To determine whether exposures to pulsed high-intensity focused ultrasound can enhance local delivery and expression of a reporter gene, administered with systemic injection of naked DNA, in tumors in mice. MATERIALS AND METHODS: The study was performed according to an approved animal protocol and in compliance with guidelines of the institutional animal care and use committee. Squamous cell carcinoma (SCC7) tumors were induced subcutaneously in both flanks of female C3H mice (n = 3) and allowed to grow to average size of 0.4 cm super(3). In each mouse, one tumor was exposed to pulsed high-intensity focused ultrasound while a second tumor served as a control. Immediately after ultrasound exposure, a solution containing a cytomegalovirus-green fluorescent protein (GFP) reporter gene construct was injected intravenously via the tail vein. The mouse was sacrificed 24 hours later. Tissue specimens were viewed with fluorescence microscopy to determine the presence of GFP expression, and Western blot analysis was performed, at which signal intensities of expressed GFP were quantitated. A paired Student t test was used to compare mean values in controls with those in treated tumors. Histologic analyses were performed with specific techniques (hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) to determine whether tumor cells had been damaged by ultrasound exposure. RESULTS: GFP expression was present in all sections of tumors that received ultrasound exposure but not in control tumors. Results of signal intensity measurement at Western blot analysis showed expressed GFP to be nine times greater in ultrasound-exposed tumors (160.2 plus or minus 24.5 [standard deviation]) than in controls (17.4 plus or minus 11.8) (P = .004, paired Student t test). Comparison of histologic sections from treated tumors with those from controls revealed no destructive effects from ultrasound exposure. CONCLUSION: Local exposure to pulsed high-intensity focused ultrasound in tumors can enhance the delivery and expression of systemically injected naked DNA. JF - Radiology AU - Dittmar, Kristin M AU - Xie, Jianwu AU - Hunter, Finie AU - Trimble, Cameron AU - Bur, Monica AU - Frenkel, Victor AU - Li, King CP AD - Department of Radiology, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Md Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 541 EP - 546 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 235 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17337134?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Pulsed+High-Intensity+Focused+Ultrasound+Enhances+Systemic+Administration+of+Naked+DNA+in+Squamous+Cell+Carcinoma+Model%3A+Initial+Experience&rft.au=Dittmar%2C+Kristin+M%3BXie%2C+Jianwu%3BHunter%2C+Finie%3BTrimble%2C+Cameron%3BBur%2C+Monica%3BFrenkel%2C+Victor%3BLi%2C+King+CP&rft.aulast=Dittmar&rft.aufirst=Kristin&rft.date=2005-05-01&rft.volume=235&rft.issue=2&rft.spage=541&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Application of fuzzy multiple goal programming on the selection for power plants considering site and environmental constraints AN - 16201084; 6434059 AB - A linear programming model with fuzzy multiple goals is presented herein from a cost-effective view to assess the impacts of environmental regulations to restrict carbon emissions on a generation plant. The model contains factors relevant to existing coal power plant scenarios, including clean coal technologies, fuel characteristics, technical alternatives, capital and operation costs, economic and regulatory environment, pipeline issues, and their bounded rationality. Moreover, to achieve the objective of cost minimization, whilst complying with the environmental regulation of carbon emission control in total scale from a long-term perspective, the model explores fuzzy multiple goals based on a power plant scheme. The proposed model was examined via implementations on a real power plant with varying characteristics through fuzzy analysis. Various designed scenarios are analyzed to reveal the option transactions in electricity generation alternatives with pipeline and distance schemes. The results obtained herein provide utility planners with further insight into the systematic analysis and potentials for the cost effectiveness in realistic applications. JF - Journal of Information & Optimization Sciences AU - Tsai, Deng Maw AU - Wang, Earl Juei AD - Department of Industrial Management, National Pingtung University of Science and Technology, 1, Hseuh-Fu Road, Nei-Pu Hsiang, Pingtung (912), Taiwan, R.O.C., dmtsai@npust.edu.tw Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 343 EP - 361 VL - 26 IS - 2 SN - 0252-2667, 0252-2667 KW - Pollution Abstracts KW - Siting criteria KW - Electric power generation KW - Fuels KW - Economics KW - Power plants KW - environmental regulations KW - Emission control KW - Coal KW - P 9000:ENVIRONMENTAL ACTION UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/16201084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Information+%26+Optimization+Sciences&rft.atitle=Application+of+fuzzy+multiple+goal+programming+on+the+selection+for+power+plants+considering+site+and+environmental+constraints&rft.au=Tsai%2C+Deng+Maw%3BWang%2C+Earl+Juei&rft.aulast=Tsai&rft.aufirst=Deng&rft.date=2005-05-01&rft.volume=26&rft.issue=2&rft.spage=343&rft.isbn=&rft.btitle=&rft.title=Journal+of+Information+%26+Optimization+Sciences&rft.issn=02522667&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Siting criteria; Fuels; Electric power generation; Economics; Power plants; environmental regulations; Emission control; Coal ER - TY - JOUR T1 - Plasmodial surface anion channel-independent phloridzin resistance in Plasmodium falciparum. AN - 67779185; 15701633 AB - The plasmodial surface anion channel (PSAC) is an unusual ion channel induced on the human red blood cell membrane after infection with the malaria parasite, Plasmodium falciparum. Because PSAC is permeant to small metabolic precursors essential for parasite growth and is present on red blood cells infected with geographically divergent parasite isolates, it may be an ideal target for future antimalarial development. Here, we used chemically induced mutagenesis and known PSAC antagonists that inhibit in vitro parasite growth to examine whether resistance mutations in PSAC can be readily induced. Stable mutants resistant to phloridzin were generated and selected within 3 weeks after treatment with 1-methyl-3-nitro-1-nitrosoguanidine. These mutants were evaluated with osmotic lysis and electrophysiological transport assays, which indicate that PSAC inhibition by phloridzin is complex with at least two different modes of inhibition. Mutants resistant to the growth inhibitory effects of phloridzin expressed PSAC activity indistinguishable from that on sensitive parasites, indicating selection of resistance via mutations in one or more other parasite targets. Failure to induce mutations in PSAC activity is consistent with a highly constrained channel protein less susceptible to resistance mutations; whether this protein is parasite- or host-encoded remains to be determined. JF - The Journal of biological chemistry AU - Desai, Sanjay A AU - Alkhalil, Abdulnaser AU - Kang, Myungsa AU - Ashfaq, Umar AU - Nguyen, My-Le AD - Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA. sdesai@niaid.nih.gov Y1 - 2005/04/29/ PY - 2005 DA - 2005 Apr 29 SP - 16861 EP - 16867 VL - 280 IS - 17 SN - 0021-9258, 0021-9258 KW - Anions KW - 0 KW - Diuretics KW - Ion Channels KW - Ions KW - Lipid Bilayers KW - Mutagens KW - Nitrosoguanidines KW - surface anion channel protein, Plasmodium falciparum KW - Furosemide KW - 7LXU5N7ZO5 KW - Phlorhizin KW - CU9S17279X KW - Index Medicus KW - Nitrosoguanidines -- pharmacology KW - Animals KW - Dose-Response Relationship, Drug KW - Algorithms KW - Diuretics -- pharmacology KW - Anions -- chemistry KW - Plasmodium falciparum KW - Electrophysiology KW - Protein Binding KW - Models, Biological KW - Osmosis KW - Mutagenesis KW - Kinetics KW - Models, Chemical KW - Furosemide -- pharmacology KW - Allosteric Site KW - Time Factors KW - Mutation KW - Lipid Bilayers -- metabolism KW - Drug Resistance KW - Phlorhizin -- pharmacology KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67779185?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Plasmodial+surface+anion+channel-independent+phloridzin+resistance+in+Plasmodium+falciparum.&rft.au=Desai%2C+Sanjay+A%3BAlkhalil%2C+Abdulnaser%3BKang%2C+Myungsa%3BAshfaq%2C+Umar%3BNguyen%2C+My-Le&rft.aulast=Desai&rft.aufirst=Sanjay&rft.date=2005-04-29&rft.volume=280&rft.issue=17&rft.spage=16861&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural basis for the function of stringent starvation protein a as a transcription factor. AN - 67769285; 15735307 AB - Stringent starvation protein A (SspA) of Escherichia coli is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. We report the crystal structure of Yersinia pestis SspA, which is 83% identical to E. coli SspA in amino acid sequence and is functionally complementary in supporting the lytic growth of phage P1 and acid resistance of an E. coli sspA mutant. The structure reveals that SspA assumes the characteristic fold of glutathione S-transferase (GST). However, SspA lacks GST activity and does not bind glutathione. Three regions of SspA are flexible, the N and C termini and the alpha2-helix. The structure also reveals a conserved surface-exposed pocket composed of residues from a loop between helices alpha3 and alpha4. The functional roles of these structural features were investigated by assessing the ability of deletion and site-directed mutants to confer acid resistance of E. coli and to activate transcription from a phage P1 late promoter, thereby supporting the lytic growth of phage P1. The results indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. The size, shape, and property of the pocket suggest that it mediates protein-protein interactions. SspA orthologs from Y. pestis, Vibrio cholerae, and Pseudomonas aeruginosa are all functional in acid resistance of E. coli, whereas only Y. pestis SspA supports phage P1 growth. JF - The Journal of biological chemistry AU - Hansen, Anne-Marie AU - Gu, Yijun AU - Li, Mi AU - Andrykovitch, Michelle AU - Waugh, David S AU - Jin, Ding Jun AU - Ji, Xinhua AD - Transcription Control Section, Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA. Y1 - 2005/04/29/ PY - 2005 DA - 2005 Apr 29 SP - 17380 EP - 17391 VL - 280 IS - 17 SN - 0021-9258, 0021-9258 KW - Adhesins, Bacterial KW - 0 KW - SspA protein, bacteria KW - Glutathione Transferase KW - EC 2.5.1.18 KW - DNA-Directed RNA Polymerases KW - EC 2.7.7.6 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Protein Structure, Secondary KW - Stereoisomerism KW - Catalytic Domain KW - Cell Proliferation KW - Mutagenesis, Site-Directed KW - Promoter Regions, Genetic KW - Databases, Protein KW - Genetic Complementation Test KW - Molecular Sequence Data KW - Vibrio cholerae -- metabolism KW - Sequence Homology, Amino Acid KW - Time Factors KW - Protein Conformation KW - Plasmids -- metabolism KW - Models, Molecular KW - Glutathione -- metabolism KW - Dimerization KW - Glutathione Transferase -- metabolism KW - Amino Acid Sequence KW - Gene Deletion KW - Pseudomonas aeruginosa -- metabolism KW - DNA-Directed RNA Polymerases -- metabolism KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Mutation KW - Escherichia coli -- metabolism KW - Adhesins, Bacterial -- metabolism KW - Yersinia pestis -- metabolism KW - Transcription, Genetic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67769285?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Structural+basis+for+the+function+of+stringent+starvation+protein+a+as+a+transcription+factor.&rft.au=Hansen%2C+Anne-Marie%3BGu%2C+Yijun%3BLi%2C+Mi%3BAndrykovitch%2C+Michelle%3BWaugh%2C+David+S%3BJin%2C+Ding+Jun%3BJi%2C+Xinhua&rft.aulast=Hansen&rft.aufirst=Anne-Marie&rft.date=2005-04-29&rft.volume=280&rft.issue=17&rft.spage=17380&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1YY7; PDB N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Expression and distribution of vanilloid receptor 1 (TRPV1) in the adult rat brain. AN - 67784458; 15857679 AB - The vanilloid receptor (TRPV1 or VR1) is a molecular integrator of various painful stimuli, including capsaicin, acid, and high temperature. It can also be activated by endogenous ligands, like the cannabinoid 1 receptor (CB1) agonist anandamide. TRPV1 is well characterized at the terminals of sensory nerves involved in the pain pathway. There is also evidence that TRPV1 is expressed in the brain but little is known about its function. Here, using commercially available specific antibodies to investigate the localization of TRPV1 in the brain of the rat, we report that TRPV1 was expressed in hippocampus, cortex, cerebellum, olfactory bulb, mesencephalon and hindbrain. Immunohistochemical analyses showed high expression in the cell bodies and dendrites of neurons in the hippocampus and in the cortex. To address the question of subcellular localization, immunoelectronmicroscopy was used. TRPV1-like staining was detected in the synapses (mostly, but not exclusively in post-synaptic dendritic spines), on the end feet of astrocytes and in pericytes. In summary, TRPV1 expression shows wide distribution in the brain of the rat, being found in astrocytes and pericytes as well as in neurons. Its localization is consistent with multiple functions within the central nervous system, including the regulation of brain vasculature. JF - Brain research. Molecular brain research AU - Tóth, Attila AU - Boczán, Judit AU - Kedei, Noémi AU - Lizanecz, Erzsébet AU - Bagi, Zsolt AU - Papp, Zoltán AU - Edes, István AU - Csiba, László AU - Blumberg, Peter M AD - Molecular Mechanisms of Tumor Promotion Section, Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. atitoth@jaguar.unideb.hu Y1 - 2005/04/27/ PY - 2005 DA - 2005 Apr 27 SP - 162 EP - 168 VL - 135 IS - 1-2 SN - 0169-328X, 0169-328X KW - Ion Channels KW - 0 KW - TRPV Cation Channels KW - Trpv1 protein, rat KW - Index Medicus KW - Animals KW - Subcellular Fractions -- ultrastructure KW - Astrocytes -- ultrastructure KW - Pericytes -- metabolism KW - Blotting, Western -- methods KW - Immunohistochemistry -- methods KW - Rats KW - Rats, Sprague-Dawley KW - Microscopy, Immunoelectron -- methods KW - Pericytes -- ultrastructure KW - In Vitro Techniques KW - Subcellular Fractions -- metabolism KW - Male KW - Astrocytes -- metabolism KW - Brain -- anatomy & histology KW - Brain -- metabolism KW - Ion Channels -- genetics KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784458?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research.+Molecular+brain+research&rft.atitle=Expression+and+distribution+of+vanilloid+receptor+1+%28TRPV1%29+in+the+adult+rat+brain.&rft.au=T%C3%B3th%2C+Attila%3BBocz%C3%A1n%2C+Judit%3BKedei%2C+No%C3%A9mi%3BLizanecz%2C+Erzs%C3%A9bet%3BBagi%2C+Zsolt%3BPapp%2C+Zolt%C3%A1n%3BEdes%2C+Istv%C3%A1n%3BCsiba%2C+L%C3%A1szl%C3%B3%3BBlumberg%2C+Peter+M&rft.aulast=T%C3%B3th&rft.aufirst=Attila&rft.date=2005-04-27&rft.volume=135&rft.issue=1-2&rft.spage=162&rft.isbn=&rft.btitle=&rft.title=Brain+research.+Molecular+brain+research&rft.issn=0169328X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-25 N1 - Date created - 2005-04-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity. AN - 67776812; 15837797 AB - Excessive accumulation of amyloid beta-peptide (Abeta) plays an early and critical role in synapse and neuronal loss in Alzheimer's Disease (AD). Increased oxidative stress is one of the mechanisms whereby Abeta induces neuronal death. Given the lessened susceptibility to oxidative stress exhibited by mice lacking p66Shc, we investigated the role of p66Shc in Abeta toxicity. Treatment of cells and primary neuronal cultures with Abeta caused apoptotic death and induced p66Shc phosphorylation at Ser36. Ectopic expression of a dominant-negative SEK1 mutant or chemical JNK inhibition reduced Abeta-induced JNK activation and p66Shc phosphorylation (Ser36), suggesting that JNK phosphorylates p66Shc. Abeta induced the phosphorylation and hence inactivation of forkhead transcription factors in a p66Shc-dependent manner. Ectopic expression of p66ShcS36A or antioxidant treatment protected cells against Abeta-induced death and reduced forkhead phosphorylation, suggesting that p66Shc phosphorylation critically influences the redox regulation of forkhead proteins and underlies Abeta toxicity. These findings underscore the potential usefulness of JNK, p66Shc, and forkhead proteins as therapeutic targets for AD. JF - The Journal of cell biology AU - Smith, Wanli W AU - Norton, Darrell D AU - Gorospe, Myriam AU - Jiang, Haibing AU - Nemoto, Shino AU - Holbrook, Nikki J AU - Finkel, Toren AU - Kusiak, John W AD - Molecular Neurobiology Unit, Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. wsmith60@jhmi.edu Y1 - 2005/04/25/ PY - 2005 DA - 2005 Apr 25 SP - 331 EP - 339 VL - 169 IS - 2 SN - 0021-9525, 0021-9525 KW - Adaptor Proteins, Signal Transducing KW - 0 KW - Amyloid beta-Peptides KW - Forkhead Transcription Factors KW - Nuclear Proteins KW - SHC1 protein, human KW - Shc Signaling Adaptor Proteins KW - Shc1 protein, mouse KW - Shc1 protein, rat KW - Src Homology 2 Domain-Containing, Transforming Protein 1 KW - Transcription Factors KW - Serine KW - 452VLY9402 KW - JNK Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - MAP Kinase Kinase 4 KW - EC 2.7.12.2 KW - Mitogen-Activated Protein Kinase Kinases KW - Index Medicus KW - Animals KW - Neurons -- metabolism KW - Oxidation-Reduction -- drug effects KW - Alzheimer Disease -- physiopathology KW - Humans KW - Mice KW - Serine -- metabolism KW - Mitogen-Activated Protein Kinase Kinases -- metabolism KW - Phosphorylation -- drug effects KW - Rats KW - Point Mutation KW - Amino Acid Substitution KW - JNK Mitogen-Activated Protein Kinases -- metabolism KW - PC12 Cells KW - Adaptor Proteins, Signal Transducing -- metabolism KW - Transcription Factors -- metabolism KW - Amyloid beta-Peptides -- toxicity KW - Signal Transduction -- drug effects KW - Apoptosis -- drug effects KW - Adaptor Proteins, Signal Transducing -- genetics KW - Nuclear Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67776812?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+cell+biology&rft.atitle=Phosphorylation+of+p66Shc+and+forkhead+proteins+mediates+Abeta+toxicity.&rft.au=Smith%2C+Wanli+W%3BNorton%2C+Darrell+D%3BGorospe%2C+Myriam%3BJiang%2C+Haibing%3BNemoto%2C+Shino%3BHolbrook%2C+Nikki+J%3BFinkel%2C+Toren%3BKusiak%2C+John+W&rft.aulast=Smith&rft.aufirst=Wanli&rft.date=2005-04-25&rft.volume=169&rft.issue=2&rft.spage=331&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+cell+biology&rft.issn=00219525&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-04-26 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Neural Transm Suppl. 2000;59:133-54 [10961426] J Neurosci. 2000 Sep 1;20(17):6442-51 [10964950] Cancer Res. 2000 Sep 15;60(18):5171-8 [11016645] Curr Biol. 2000 Oct 5;10(19):1201-4 [11050388] Trends Biochem Sci. 2002 Jul;27(7):352-60 [12114024] J Neurochem. 2001 Jan;76(2):435-41 [11208906] Brain. 2002 Sep;125(Pt 9):2036-43 [12183349] Neurobiol Aging. 2002 Sep-Oct;23(5):655-64 [12392766] J Biol Chem. 2002 Oct 25;277(43):40302-8 [12192006] Ann N Y Acad Sci. 2004 Mar;1012:153-63 [15105262] Cell. 2004 May 14;117(4):421-6 [15137936] Neurobiol Aging. 2004 May-Jun;25(5):563-8 [15172731] Nature. 2004 Jun 3;429(6991):562-6 [15175753] Science. 2004 Mar 26;303(5666):2011-5 [14976264] J Neurochem. 2004 Jun;89(6):1528-36 [15189356] Science. 2004 Jul 16;305(5682):361 [15192154] Biochim Biophys Acta. 1992 Feb 3;1133(3):275-85 [1737061] Cell. 1992 Jul 10;70(1):93-104 [1623525] Nature. 1992 Dec 17;360(6405):689-92 [1465135] J Biol Chem. 1993 Mar 15;268(8):5748-53 [8449939] J Biol Chem. 1993 Apr 15;268(11):7610-2 [7681824] Nature. 1993 May 6;363(6424):45-51 [8479536] Science. 1993 Jun 25;260(5116):1953-5 [8316835] J Biol Chem. 1993 Oct 15;268(29):21463-5 [7691810] J Biol Chem. 1994 Jan 14;269(2):1143-8 [8288573] Cell. 1994 Jun 17;77(6):817-27 [8004671] Cell. 1994 Sep 23;78(6):949-61 [7923364] Science. 1994 Dec 16;266(5192):1862-5 [7527937] Biochem J. 1995 Oct 1;311 ( Pt 1):1-16 [7575439] J Neurochem. 1995 Oct;65(4):1487-98 [7561842] Nature. 1996 Mar 7;380(6569):75-9 [8598911] Trends Biochem Sci. 1996 Jul;21(7):257-61 [8755247] J Neurosci. 1996 Mar 1;16(5):1710-9 [8774439] EMBO J. 1997 Feb 17;16(4):706-16 [9049300] J Neurosci. 1997 Apr 15;17(8):2653-7 [9092586] Endocrinology. 1997 Jun;138(6):2474-80 [9165038] Neurobiol Dis. 1996 Feb;3(1):3-15 [9173909] Nature. 1997 Oct 30;389(6654):994-9 [9353126] Science. 1997 Nov 14;278(5341):1319-22 [9360933] Mol Cell Biol. 1999 Jan;19(1):751-63 [9858598] Neurobiol Aging. 1998 Sep-Oct;19(5):393-400 [9880041] Cell. 1999 Mar 19;96(6):857-68 [10102273] Nature. 1999 Apr 15;398(6728):630-4 [10217147] J Biol Chem. 1999 Jun 11;274(24):16741-6 [10358014] J Biol Chem. 1999 Jun 11;274(24):17184-92 [10358076] J Neurosci Res. 1999 Jun 1;56(5):457-70 [10369213] Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7421-6 [10377430] Biochemistry. 1999 Jun 15;38(24):7609-16 [10386999] Mol Cell Biol. 2000 Dec;20(23):8969-82 [11073996] Curr Opin Genet Dev. 2000 Dec;10(6):668-74 [11088019] Mol Cell Biol. 2000 Dec;20(24):9138-48 [11094066] Brain Res Mol Brain Res. 2000 Dec 28;85(1-2):221-33 [11146125] Physiol Rev. 2001 Apr;81(2):741-66 [11274343] Nat Genet. 2001 Jun;28(2):139-45 [11381260] Curr Opin Neurobiol. 2001 Jun;11(3):297-305 [11399427] J Biol Chem. 2001 Dec 21;276(51):48332-6 [11602589] Mol Cell Biol. 2002 Apr;22(7):2025-36 [11884591] Science. 2002 Mar 29;295(5564):2450-2 [11884717] J Neurochem. 2002 Mar;80(6):1049-61 [11953455] Science. 2002 Apr 19;296(5567):530-4 [11964479] J Biol Chem. 2002 May 17;277(20):17649-56 [11882652] Oncogene. 2002 May 30;21(24):3872-8 [12032825] J Cell Mol Med. 2001 Jan-Mar;5(1):1-17 [12067447] J Biol Chem. 2003 Jan 31;278(5):3437-45 [12435748] Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11836-41 [10518537] Nature. 1999 Nov 18;402(6759):309-13 [10580504] J Biol Chem. 1999 Dec 24;274(52):37111-6 [10601271] Nature. 2000 Apr 13;404(6779):782-7 [10783894] IUBMB Life. 2000 Mar;49(3):177-80 [10868907] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure. AN - 67753758; 15701627 AB - Mouse fibroblasts, deficient in DNA polymerase beta, are hypersensitive to monofunctional DNA methylating agents such as methyl methanesulfonate (MMS). Both wild-type and, in particular, repair-deficient DNA polymerase beta null cells are highly sensitized to the cytotoxic effects of MMS by 4-amino-1,8-naphthalimide (4-AN), an inhibitor of poly(ADP-ribose) polymerase (PARP) activity. Experiments with synchronized cells suggest that exposure during S-phase of the cell cycle is required for the 4-AN effect. 4-AN elicits a similar extreme sensitization to the thymidine analog, 5-hydroxymethyl-2'-deoxyuridine, implicating the requirement for an intermediate of DNA repair. In PARP-1-expressing fibroblasts treated with a combination of MMS and 4-AN, a complete inhibition of DNA synthesis is apparent after 4 h, and by 24 h, all cells are arrested in S-phase of the cell cycle. Continuous incubation with 4-AN is required to maintain the cell cycle arrest. Caffeine, an inhibitor of the upstream checkpoint kinases ATM (ataxia telangiectasia-mutated) and ATR (ATM and Rad3-related), has no effect on the early inhibition of DNA synthesis, but cells are no longer able to maintain the block after 8 h. Instead, the addition of caffeine leads to arrest of cells in G(2)/M rather than S-phase after 24 h. Analysis of signaling pathways in cell extracts reveals an activation of Chk1 after treatment with MMS and 4-AN, which can be suppressed by caffeine. Our results suggest that inhibition of PARP activity results in sensitization to MMS through maintenance of an ATR and Chk1-dependent S-phase checkpoint. JF - The Journal of biological chemistry AU - Horton, Julie K AU - Stefanick, Donna F AU - Naron, Jana M AU - Kedar, Padmini S AU - Wilson, Samuel H AD - Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/04/22/ PY - 2005 DA - 2005 Apr 22 SP - 15773 EP - 15785 VL - 280 IS - 16 SN - 0021-9258, 0021-9258 KW - Antineoplastic Agents, Alkylating KW - 0 KW - Naphthalimides KW - Poly(ADP-ribose) Polymerase Inhibitors KW - Quinolones KW - 4-amino-1,8-naphthalimide KW - 1742-95-6 KW - DNA KW - 9007-49-2 KW - 1-Naphthylamine KW - 9753I242R5 KW - Methyl Methanesulfonate KW - AT5C31J09G KW - Poly(ADP-ribose) Polymerases KW - EC 2.4.2.30 KW - Index Medicus KW - Fibroblasts -- drug effects KW - Animals KW - DNA Methylation KW - Antineoplastic Agents, Alkylating -- pharmacology KW - Mice KW - Fibroblasts -- metabolism KW - Quinolones -- pharmacology KW - Methyl Methanesulfonate -- pharmacology KW - DNA Damage -- drug effects KW - Signal Transduction -- physiology KW - Poly(ADP-ribose) Polymerases -- genetics KW - 1-Naphthylamine -- analogs & derivatives KW - DNA -- metabolism KW - Cell Cycle -- physiology KW - Poly(ADP-ribose) Polymerases -- metabolism KW - 1-Naphthylamine -- pharmacology KW - Cell Cycle -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67753758?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Poly%28ADP-ribose%29+polymerase+activity+prevents+signaling+pathways+for+cell+cycle+arrest+after+DNA+methylating+agent+exposure.&rft.au=Horton%2C+Julie+K%3BStefanick%2C+Donna+F%3BNaron%2C+Jana+M%3BKedar%2C+Padmini+S%3BWilson%2C+Samuel+H&rft.aulast=Horton&rft.aufirst=Julie&rft.date=2005-04-22&rft.volume=280&rft.issue=16&rft.spage=15773&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Male breast cancer incidence among atomic bomb survivors. AN - 67762536; 15840883 AB - To learn more about the role of ionizing radiation in the development of male breast cancer, we evaluated male breast cancer incidence among 45 880 male members of the Life Span Study cohort of Japanese atomic bomb survivors. Male breast cancers, diagnosed between January 1, 1958, and December 31, 1998, were identified through the Hiroshima and Nagasaki Tumor Registries. Nine male breast cancers were diagnosed among exposed Life Span Study members (crude rate = 1.8 per 100,000 person-years), and three were diagnosed among nonexposed cohort members (crude rate = 0.5 per 100,000 person-years). A statistically significant dose-response relation was observed (excess relative risk per sievert = 8, 95% confidence interval = 0.8 to 48; P = .01). Our finding of a statistically significant association between ionizing radiation and male breast cancer incidence adds to the very limited information that shows an association between radiation exposure and an increased risk of male breast cancer. JF - Journal of the National Cancer Institute AU - Ron, Elaine AU - Ikeda, Takayoshi AU - Preston, Dale L AU - Tokuoka, Shoji AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, 6120 Executive Blvd., MSC 7362, Bethesda, MD 20892-7362, USA. eron@mail.nih.gov Y1 - 2005/04/20/ PY - 2005 DA - 2005 Apr 20 SP - 603 EP - 605 VL - 97 IS - 8 KW - Index Medicus KW - Japan -- epidemiology KW - Radiation Dosage KW - Humans KW - Incidence KW - Confidence Intervals KW - Aged KW - Middle Aged KW - Time Factors KW - Male KW - Risk Assessment KW - Survivors -- statistics & numerical data KW - Breast Neoplasms, Male -- epidemiology KW - Nuclear Warfare KW - Neoplasms, Radiation-Induced -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67762536?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Male+breast+cancer+incidence+among+atomic+bomb+survivors.&rft.au=Ron%2C+Elaine%3BIkeda%2C+Takayoshi%3BPreston%2C+Dale+L%3BTokuoka%2C+Shoji&rft.aulast=Ron&rft.aufirst=Elaine&rft.date=2005-04-20&rft.volume=97&rft.issue=8&rft.spage=603&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=1460-2105&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-25 N1 - Date created - 2005-04-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase II clinical trial of ixabepilone (BMS-247550), an epothilone B analog, in metastatic and locally advanced breast cancer. AN - 67757381; 15837987 AB - Ixabepilone (BMS-247550) is an epothilone B analog that stabilizes microtubules and has antitumor activity in taxane-refractory patients in phase I studies. In a phase II trial, we evaluated the efficacy and safety of ixabepilone in women with metastatic and locally advanced breast cancer. Breast cancer patients with measurable disease who had paclitaxel and/or docetaxel as prior neoadjuvant, adjuvant, or metastatic therapy were treated with ixabepilone at 6 mg/m2/d intravenously on days 1 through 5 every 3 weeks. Levels of glutamate (glu) -terminated and acetylated alpha-tubulin, markers of microtubule stabilization, were detected by Western blot and by immunohistochemistry in a subset of matched pre- and post-treatment tumor biopsies. Thirty-seven patients received 153 cycles of ixabepilone. The best responses were a complete response in one patient (3%), partial responses in seven patients (19%), and stable disease in 13 patients (35%). Grade 3 and 4 toxicities included neutropenia (35%), febrile neutropenia (14%), fatigue (14%), diarrhea (11%), nausea/vomiting (5%), myalgia/arthralgia (3%), and sensory neuropathy (3%). Two patients were removed from study because of prolonged grade 2 or 3 neurotoxicity, and three patients were removed from study for other grade 3 and 4 nonhematologic toxicities. Compared with baseline levels, levels of both glu-terminated and acetylated alpha-tubulin were increased in tumor biopsies performed after ixabepilone therapy. An objective response was seen in 22% of the patients in a population who had been previously treated with a taxane. Sensory neuropathy was mild with grade 3 neurotoxicity rarely seen. Microtubule stabilization occurred in tumor biopsies after treatment with ixabepilone. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Low, Jennifer A AU - Wedam, Suparna B AU - Lee, James J AU - Berman, Arlene W AU - Brufsky, Adam AU - Yang, Sherry X AU - Poruchynsky, Marianne S AU - Steinberg, Seth M AU - Mannan, Nitin AU - Fojo, Tito AU - Swain, Sandra M AD - Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20889-5015, USA. Y1 - 2005/04/20/ PY - 2005 DA - 2005 Apr 20 SP - 2726 EP - 2734 VL - 23 IS - 12 SN - 0732-183X, 0732-183X KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Epothilones KW - Taxoids KW - docetaxel KW - 15H5577CQD KW - ixabepilone KW - K27005NP0A KW - Paclitaxel KW - P88XT4IS4D KW - Index Medicus KW - Infusions, Intravenous KW - Humans KW - Antineoplastic Agents, Phytogenic -- therapeutic use KW - Nervous System -- drug effects KW - Adult KW - Treatment Outcome KW - Paclitaxel -- therapeutic use KW - Middle Aged KW - Taxoids -- therapeutic use KW - Nervous System -- pathology KW - Immunohistochemistry KW - Female KW - Breast Neoplasms -- drug therapy KW - Epothilones -- therapeutic use KW - Breast Neoplasms -- pathology KW - Epothilones -- adverse effects KW - Epothilones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67757381?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Phase+II+clinical+trial+of+ixabepilone+%28BMS-247550%29%2C+an+epothilone+B+analog%2C+in+metastatic+and+locally+advanced+breast+cancer.&rft.au=Low%2C+Jennifer+A%3BWedam%2C+Suparna+B%3BLee%2C+James+J%3BBerman%2C+Arlene+W%3BBrufsky%2C+Adam%3BYang%2C+Sherry+X%3BPoruchynsky%2C+Marianne+S%3BSteinberg%2C+Seth+M%3BMannan%2C+Nitin%3BFojo%2C+Tito%3BSwain%2C+Sandra+M&rft.aulast=Low&rft.aufirst=Jennifer&rft.date=2005-04-20&rft.volume=23&rft.issue=12&rft.spage=2726&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-19 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase. AN - 67751565; 15837423 AB - Inositol hexakisphosphate and other inositol high polyphosphates have diverse and critical roles in eukaryotic regulatory pathways. Inositol 1,3,4-trisphosphate 5/6-kinase catalyzes the rate-limiting step in inositol high polyphosphate synthesis in animals. This multifunctional enzyme also has inositol 3,4,5,6-tetrakisphosphate 1-kinase and other activities. The structure of an archetypal family member, from Entamoeba histolytica, has been determined to 1.2 A resolution in binary and ternary complexes with nucleotide, substrate, and product. The structure reveals an ATP-grasp fold. The inositol ring faces ATP edge-on such that the 5- and 6-hydroxyl groups are nearly equidistant from the ATP gamma-phosphate in catalytically productive phosphoacceptor positions and explains the unusual dual site specificity of this kinase. Inositol tris- and tetrakisphosphates interact via three phosphate binding subsites and one solvent-exposed site that could in principle be occupied by 18 different substrates, explaining the mechanisms for the multiple specificities and catalytic activities of this enzyme. JF - Molecular cell AU - Miller, Gregory J AU - Wilson, Monita P AU - Majerus, Philip W AU - Hurley, James H AD - Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, United States Department of Health and Human Services, Bethesda, Maryland 20892, USA. Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 201 EP - 212 VL - 18 IS - 2 SN - 1097-2765, 1097-2765 KW - Inositol Phosphates KW - 0 KW - Ligands KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - inositol 1,3,4-trisphosphate KW - 98102-63-7 KW - Phosphotransferases (Alcohol Group Acceptor) KW - EC 2.7.1.- KW - myo-inositol-trisphosphate 6-kinase KW - EC 2.7.1.134 KW - Magnesium KW - I38ZP9992A KW - Index Medicus KW - Molecular Structure KW - Animals KW - Stereoisomerism KW - Protein Structure, Secondary KW - Electrons KW - Models, Molecular KW - Inositol Phosphates -- metabolism KW - DNA Mutational Analysis KW - Humans KW - Amino Acid Sequence KW - Entamoeba histolytica -- chemistry KW - Protein Binding KW - Binding Sites KW - Mutagenesis, Site-Directed KW - Magnesium -- metabolism KW - Adenosine Triphosphate -- metabolism KW - Molecular Sequence Data KW - Substrate Specificity KW - Molecular Conformation KW - Sequence Homology, Amino Acid KW - Protein Structure, Tertiary KW - Spodoptera -- cytology KW - Adenosine Diphosphate -- metabolism KW - Phosphotransferases (Alcohol Group Acceptor) -- chemistry KW - Phosphotransferases (Alcohol Group Acceptor) -- biosynthesis KW - Crystallography, X-Ray KW - Phosphotransferases (Alcohol Group Acceptor) -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67751565?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cell&rft.atitle=Specificity+determinants+in+inositol+polyphosphate+synthesis%3A+crystal+structure+of+inositol+1%2C3%2C4-trisphosphate+5%2F6-kinase.&rft.au=Miller%2C+Gregory+J%3BWilson%2C+Monita+P%3BMajerus%2C+Philip+W%3BHurley%2C+James+H&rft.aulast=Miller&rft.aufirst=Gregory&rft.date=2005-04-15&rft.volume=18&rft.issue=2&rft.spage=201&rft.isbn=&rft.btitle=&rft.title=Molecular+cell&rft.issn=10972765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-31 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1Z2P; PDB; 1Z2O; 1Z2N N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Colorectal papillomavirus infection in patients with colorectal cancer. AN - 67749292; 15837733 AB - Infection with human papillomaviruses (HPV) is associated with the development of cervical cancer, but whether HPVs have a role in colorectal cancer remains controversial. To determine the relationship between HPV and colorectal cancer, we did a retrospective, controlled study using tumor and tumor-adjacent colorectal tissues dissected from patients with colorectal cancer, as well as colorectal tissues from control individuals with no cancer. The samples were processed in a blinded fashion for nested PCR and in situ PCR detection of HPV DNAs. The PCR products were gel-purified and sequenced for HPV genotyping. We found that colorectal tissues from 28 of 55 (51%) patients with colorectal cancer were positive for HPV DNA. Colorectal tissues from all 10 control individuals were negative for HPV DNA (P = 0.0034). Of the 107 usable (GAPDH(+)) samples collected as paired colorectal tissues (tumor and tumor-adjacent tissues) from the patients, 38 (36%) had HPV16 (n = 31), HPV18 (n = 5), or HPV45 (n = 2), with HPV DNA in both tumor and tumor-adjacent tissues of 10 paired samples, 13 in only the tumor, and 5 in only tumor-adjacent tissues. In situ PCR detection of the tumor tissues confirmed the presence of HPV DNA in tumor cells. Our results suggest that colorectal HPV infection is common in patients with colorectal cancer, albeit at a low DNA copy number, with HPV16 being the most prevalent type. HPV infection may play a role in colorectal carcinogenesis. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Bodaghi, Sohrab AU - Yamanegi, Koji AU - Xiao, Shu-Yuan AU - Da Costa, Maria AU - Palefsky, Joel M AU - Zheng, Zhi-Ming AD - HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 2862 EP - 2867 VL - 11 IS - 8 SN - 1078-0432, 1078-0432 KW - DNA, Viral KW - 0 KW - Index Medicus KW - Rectum -- virology KW - Colon -- virology KW - Reproducibility of Results KW - Colon -- pathology KW - Humans KW - Aged KW - Carcinoma, Squamous Cell -- virology KW - Adenocarcinoma -- pathology KW - Rectum -- pathology KW - Aged, 80 and over KW - Carcinoma, Squamous Cell -- pathology KW - Polymerase Chain Reaction -- methods KW - Adult KW - Middle Aged KW - DNA, Viral -- isolation & purification KW - Adenocarcinoma -- virology KW - DNA, Viral -- genetics KW - Male KW - Female KW - Papillomavirus Infections -- pathology KW - Colorectal Neoplasms -- pathology KW - Papillomavirus Infections -- virology KW - Colorectal Neoplasms -- ethnology KW - Papillomaviridae -- genetics KW - Colorectal Neoplasms -- virology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67749292?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Colorectal+papillomavirus+infection+in+patients+with+colorectal+cancer.&rft.au=Bodaghi%2C+Sohrab%3BYamanegi%2C+Koji%3BXiao%2C+Shu-Yuan%3BDa+Costa%2C+Maria%3BPalefsky%2C+Joel+M%3BZheng%2C+Zhi-Ming&rft.aulast=Bodaghi&rft.aufirst=Sohrab&rft.date=2005-04-15&rft.volume=11&rft.issue=8&rft.spage=2862&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-29 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Natl Cancer Inst Monogr. 2003;(31):52-6 [12807946] Cancer Res. 2003 Nov 1;63(21):7515-9 [14612553] Arch Surg. 1990 Jul;125(7):862-5 [2164371] Science. 1991 Jun 21;252(5013):1643-51 [2047872] Am J Surg Pathol. 1992 Mar;16(3):269-75 [1317997] Gastroenterology. 2001 Mar;120(4):988-94 [11231953] J Natl Cancer Inst. 2000 May 3;92(9):709-20 [10793107] Cancer Res. 1999 Dec 15;59(24):6132-6 [10626803] J Clin Microbiol. 2000 Jan;38(1):357-61 [10618116] Eur J Cancer Prev. 1998 Aug;7(4):305-13 [9806119] N Engl J Med. 1997 Nov 6;337(19):1350-8 [9358129] J Gen Virol. 1995 Apr;76 ( Pt 4):1057-62 [9049358] Gut. 1995 Jul;37(1):87-90 [7672688] J Clin Microbiol. 1995 Apr;33(4):901-5 [7790457] J Surg Oncol. 1992 Sep;51(1):5-7 [1325576] Am J Surg. 1993 Dec;166(6):738-40; discussion 741-2 [8273860] Int J Cancer. 2003 Apr 10;104(3):336-44 [12569557] CA Cancer J Clin. 2003 Jan-Feb;53(1):5-26 [12568441] Clin Cancer Res. 2002 Oct;8(10):3187-92 [12374687] J Clin Pathol. 2002 Oct;55(10):721-8 [12354793] Int J Colorectal Dis. 2002 Nov;17(6):396-401 [12355215] J Med Virol. 2002 Nov;68(3):412-6 [12226830] Nat Rev Cancer. 2002 May;2(5):342-50 [12044010] J Infect Dis. 2002 May 1;185(9):1229-37 [12001039] J Clin Virol. 2001 May;21(2):129-34 [11378493] J Microbiol Immunol Infect. 2001 Jun;34(2):87-91 [11456365] Pathologica. 2001 Oct;93(5):531-4 [11725354] Cancer Res. 2001 Apr 1;61(7):2799-803 [11306446] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of prolidase activity by nickel causes decreased growth of proline auxotrophic CHO cells. AN - 67560817; 15696600 AB - Occupational exposure to nickel has been epidemiologically linked to increased cancer risk in the respiratory tract. Nickel-induced cell transformation is associated with both genotoxic and epigenetic mechanisms that are poorly understood. Prolidase [E.C.3.4.13.9] is a cytosolic Mn(II)-activated metalloproteinase that specifically hydrolyzes imidodipeptides with C-terminal proline or hydroxyproline and plays an important role in the recycling of proline for protein synthesis and cell growth. Prolidase also provides free proline as substrate for proline oxidase, whose gene is activated by p53 during apoptosis. The inhibition of prolidase activity by nickel has not yet been studied. We first showed that Ni(II) chloride specifically inhibited prolidase activity in CHO-K1 cells in situ. This interpretation was possible because CHO-K1 cells are proline auxotrophs requiring added free proline or proline released from added Gly-Pro by prolidase. In a dose-dependent fashion, Ni(II) inhibited growth on Gly-Pro but did not inhibit growth on proline, thereby showing inhibition of prolidase in situ in the absence of nonspecific toxicity. Studies using cell-free extracts showed that Ni(II) inhibited prolidase activity when present during prolidase activation with Mn(II) or during incubation with Gly-Pro. In kinetic studies, we found that Ni(II) inhibition of prolidase varied with respect to Mn(II) concentration. Analysis of these data suggested that increasing concentrations of Mn(II) stabilized the enzyme protein against Ni(II) inhibition. Because prolidase is an important enzyme in collagen metabolism, inhibition of the enzyme activity by nickel could alter the metabolism of collagen and other matrix proteins, and thereby alter cell-matrix and cell-cell interactions involved in gene expression, genomic stability, cellular differentiation, and cell proliferation. JF - Journal of cellular biochemistry AU - Miltyk, Wojciech AU - Surazynski, Arkadiusz AU - Kasprzak, Kazimierz S AU - Fivash, Matthew J AU - Buzard, Gregory S AU - Phang, James M AD - Metabolism and Cancer Susceptibility Section, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA. Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 1210 EP - 1217 VL - 94 IS - 6 SN - 0730-2312, 0730-2312 KW - Manganese KW - 42Z2K6ZL8P KW - Nickel KW - 7OV03QG267 KW - Dipeptidases KW - EC 3.4.13.- KW - proline dipeptidase KW - EC 3.4.13.9 KW - Index Medicus KW - Animals KW - Manganese -- pharmacology KW - CHO Cells KW - Cricetinae KW - Nickel -- pharmacology KW - Dipeptidases -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67560817?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cellular+biochemistry&rft.atitle=Inhibition+of+prolidase+activity+by+nickel+causes+decreased+growth+of+proline+auxotrophic+CHO+cells.&rft.au=Miltyk%2C+Wojciech%3BSurazynski%2C+Arkadiusz%3BKasprzak%2C+Kazimierz+S%3BFivash%2C+Matthew+J%3BBuzard%2C+Gregory+S%3BPhang%2C+James+M&rft.aulast=Miltyk&rft.aufirst=Wojciech&rft.date=2005-04-15&rft.volume=94&rft.issue=6&rft.spage=1210&rft.isbn=&rft.btitle=&rft.title=Journal+of+cellular+biochemistry&rft.issn=07302312&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-11 N1 - Date created - 2005-03-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Supplementary analysis of probabilities at the termination of a group sequential phase II trial. AN - 67523574; 15565737 AB - We consider estimation of various probabilities after termination of a group sequential phase II trial. A motivating example is that the stopping rule of a phase II oncologic trial is determined solely based on response to a drug treatment, and at the end of the trial estimating the rate of toxicity and response is desirable. The conventional maximum likelihood estimator (sample proportion) of a probability is shown to be biased, and two alternative estimators are proposed to correct for bias, a bias-reduced estimator obtained by using Whitehead's bias-adjusted approach, and an unbiased estimator from the Rao-Blackwell method of conditioning. All three estimation procedures are shown to have certain invariance property in bias. Moreover, estimators of a probability and their bias and precision can be evaluated through the observed response rate and the stage at which the trial stops, thus avoiding extensive computation. Copyright 2004 John Wiley & Sons, Ltd. JF - Statistics in medicine AU - Liu, Aiyi AU - Wu, Chengqing AU - Yu, Kai F AU - Gehan, Edmund AD - Biometry and Mathematical Statistics Branch, Department of Health and Human Services, National Institute of Child Health and Human Development, 6100 Executive Boulevard, Rockville, MD 20892, USA. liua@mail.nih.gov Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 1009 EP - 1027 VL - 24 IS - 7 SN - 0277-6715, 0277-6715 KW - Index Medicus KW - Computer Simulation KW - Humans KW - Drug-Related Side Effects and Adverse Reactions KW - Bias (Epidemiology) KW - Data Interpretation, Statistical KW - Clinical Trials, Phase III as Topic -- methods KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67523574?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Statistics+in+medicine&rft.atitle=Supplementary+analysis+of+probabilities+at+the+termination+of+a+group+sequential+phase+II+trial.&rft.au=Liu%2C+Aiyi%3BWu%2C+Chengqing%3BYu%2C+Kai+F%3BGehan%2C+Edmund&rft.aulast=Liu&rft.aufirst=Aiyi&rft.date=2005-04-15&rft.volume=24&rft.issue=7&rft.spage=1009&rft.isbn=&rft.btitle=&rft.title=Statistics+in+medicine&rft.issn=02776715&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CONF T1 - Hepatitis C virus infection and substance abuse: medical management and developing models of integrated care--an introduction. AN - 67515709; 15768332 JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Kresina, Thomas F AU - Khalsa, Jag AU - Cesari, Helen AU - Francis, Henry Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - S259 EP - S262 VL - 40 Suppl 5 KW - Index Medicus KW - Models, Organizational KW - Risk Factors KW - Mental Disorders -- epidemiology KW - Humans KW - Delivery of Health Care -- classification KW - Treatment Outcome KW - Mental Disorders -- complications KW - Health Services Accessibility KW - Comorbidity KW - Hepatitis C -- therapy KW - HIV Infections -- complications KW - Delivery of Health Care, Integrated -- organization & administration KW - Hepatitis C -- complications KW - HIV Infections -- therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- epidemiology KW - Hepatitis C -- epidemiology KW - Substance Abuse, Intravenous -- complications KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67515709?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Hepatitis+C+virus+infection+and+substance+abuse%3A+medical+management+and+developing+models+of+integrated+care--an+introduction.&rft.au=Kresina%2C+Thomas+F%3BKhalsa%2C+Jag%3BCesari%2C+Helen%3BFrancis%2C+Henry&rft.aulast=Kresina&rft.aufirst=Thomas&rft.date=2005-04-15&rft.volume=40+Suppl+5&rft.issue=&rft.spage=S259&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-10-06 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Daily versus As-Needed Corticosteroids for Mild Persistent Asthma AN - 223931590; 15829533 AB - Background Although guidelines recommend daily therapy for patients with mild persistent asthma, prescription patterns suggest that most such patients use these so-called controller therapies intermittently. In patients with mild persistent asthma, we evaluated the efficacy of intermittent short-course corticosteroid treatment guided by a symptom-based action plan alone or in addition to daily treatment with either inhaled budesonide or oral zafirlukast over a one-year period. Methods In a double-blind trial, 225 adults underwent randomization. The primary outcome was morning peak expiratory flow (PEF). Other outcomes included the forced expiratory volume in one second (FEV1 ) before and after bronchodilator treatment, the frequency of exacerbations, the degree of asthma control, the number of symptom-free days, and the quality of life. Results The three treatments produced similar increases in morning PEF (7.1 to 8.3 percent; approximately 32 liters per minute; P=0.90) and similar rates of asthma exacerbations (P=0.24), even though the intermittent-treatment group took budesonide, on average, for only 0.5 week of the year. As compared with intermittent therapy or daily zafirlukast therapy, daily budesonide therapy produced greater improvements in pre-bronchodilator FEV1 (P=0.005), bronchial reactivity (P<0.001), the percentage of eosinophils in sputum (P=0.007), exhaled nitric oxide levels (P=0.006), scores for asthma control (P<0.001), and the number of symptom-free days (P=0.03), but not in post-bronchodilator FEV1 (P=0.29) or in the quality of life (P=0.18). Daily zafirlukast therapy did not differ significantly from intermittent treatment in any outcome measured. Conclusions It may be possible to treat mild persistent asthma with short, intermittent courses of inhaled or oral corticosteroids taken when symptoms worsen. Further studies are required to determine whether this novel approach to treatment should be recommended. JF - The New England Journal of Medicine AU - Boushey, Homer A, MD AU - Sorkness, Christine A, PharmD AU - King, Tonya S, PhD AU - Sullivan, Sean D, PhD AU - Fahy, John V, MD AU - Lazarus, Stephen C, MD AU - Chinchilli, Vernon M, PhD AU - Craig, Timothy J, DO AU - Dimango, Emily A, MD AU - Deykin, Aaron, MD AU - Fagan, Joanne K, PhD AU - Fish, James E, MD AU - Ford, Jean G, MD AU - Kraft, Monica, MD AU - Lemanske, Robert F, Jr, MD AU - Leone, Frank T, MD AU - Martin, Richard J, MD AU - Mauger, Elizabeth A, PhD AU - Pesola, Gene R, MD, MPH AU - Peters, Stephen P, MD, PhD AU - Rollings, Nancy J, MEd AU - Szefler, Stanley J, MD AU - Wechsler, Michael E, MD AU - Israel, Elliot, MD Y1 - 2005/04/14/ PY - 2005 DA - 2005 Apr 14 SP - 1519 EP - 28 CY - Boston PB - Massachusetts Medical Society VL - 352 IS - 15 SN - 00284793 KW - Medical Sciences KW - Adrenal Cortex Hormones KW - Anti-Asthmatic Agents KW - Bronchodilator Agents KW - Tosyl Compounds KW - zafirlukast KW - Budesonide KW - Asthma KW - Airway management KW - Administration, Oral KW - Asthma -- classification KW - Drug Administration Schedule KW - Double-Blind Method KW - Bronchodilator Agents -- administration & dosage KW - Humans KW - Peak Expiratory Flow Rate -- drug effects KW - Asthma -- physiopathology KW - Adult KW - Administration, Inhalation KW - Female KW - Forced Expiratory Volume -- drug effects KW - Male KW - Asthma -- drug therapy KW - Anti-Asthmatic Agents -- administration & dosage KW - Adrenal Cortex Hormones -- administration & dosage KW - Budesonide -- administration & dosage KW - Tosyl Compounds -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/223931590?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+Journal+of+Medicine&rft.atitle=Daily+versus+As-Needed+Corticosteroids+for+Mild+Persistent+Asthma&rft.au=Boushey%2C+Homer+A%2C+MD%3BSorkness%2C+Christine+A%2C+PharmD%3BKing%2C+Tonya+S%2C+PhD%3BSullivan%2C+Sean+D%2C+PhD%3BFahy%2C+John+V%2C+MD%3BLazarus%2C+Stephen+C%2C+MD%3BChinchilli%2C+Vernon+M%2C+PhD%3BCraig%2C+Timothy+J%2C+DO%3BDimango%2C+Emily+A%2C+MD%3BDeykin%2C+Aaron%2C+MD%3BFagan%2C+Joanne+K%2C+PhD%3BFish%2C+James+E%2C+MD%3BFord%2C+Jean+G%2C+MD%3BKraft%2C+Monica%2C+MD%3BLemanske%2C+Robert+F%2C+Jr%2C+MD%3BLeone%2C+Frank+T%2C+MD%3BMartin%2C+Richard+J%2C+MD%3BMauger%2C+Elizabeth+A%2C+PhD%3BPesola%2C+Gene+R%2C+MD%2C+MPH%3BPeters%2C+Stephen+P%2C+MD%2C+PhD%3BRollings%2C+Nancy+J%2C+MEd%3BSzefler%2C+Stanley+J%2C+MD%3BWechsler%2C+Michael+E%2C+MD%3BIsrael%2C+Elliot%2C+MD&rft.aulast=Boushey&rft.aufirst=Homer&rft.date=2005-04-14&rft.volume=352&rft.issue=15&rft.spage=1519&rft.isbn=&rft.btitle=&rft.title=The+New+England+Journal+of+Medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Central N1 - Copyright - Copyright © 2005 Massachusetts Medical Society. All rights reserved. N1 - Document feature - Graphs; Tables; References N1 - Last updated - 2014-04-29 N1 - CODEN - NEJMAG ER - TY - JOUR T1 - Metabotropic glutamate receptors and dopamine receptors cooperate to enhance extracellular signal-regulated kinase phosphorylation in striatal neurons. AN - 67743234; 15829628 AB - Striatal medium spiny neurons are an important site of convergence for signaling mediated by the neurotransmitters dopamine and glutamate. We report that in striatal neurons in primary culture, signaling through group I metabotropic glutamate receptors (mGluRs) 1/5 and the D1 class of dopamine receptors (DRs) 1/5 converges to increase phosphorylation of the mitogen-activated protein kinase ERK2 (extracellular signal-regulated kinase 2). Induction of mitogen-activated protein kinase kinase-dependent signaling cascades by either mGluR1/5 or DR1/5 gave rise to increases in phosphorylation of ERK2. Coactivation of mGluR1/5 and DR1/5 with (S)-3,5-dihydroxyphenylglycine and (+)-1-phenyl-2,3,4,5-tetrahydro-(1H)-3-benzazepine-7,8-diol hydrochloride enhanced the phosphorylation of ERK2. This interaction between mGluR1/5 and DR1/5 required protein kinase C (PKC), because the PKC inhibitors calphostin C, bisindolylmaleimide I, and Gö6976 blocked DR1/5-enhanced phosphorylation of ERK2. Use of the phosphatase inhibitors calyculin and okadaic acid indicated that inhibition of protein phosphatases 1 and 2A dramatically enhanced ERK2 phosphorylation by mGluR1/5. Coactivation of mGluR1/5 and DR1/5 also enhanced cAMP-response element binding protein (CREB) phosphorylation (compared with each receptor agonist alone) but did not enhance CREB-mediated transcriptional activity. Thus, signal transduction pathways activated by DR1/5 and mGluR5 interact to modify downstream events in striatal neurons while retaining numerous regulatory checkpoints. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Voulalas, Pamela J AU - Holtzclaw, Lynne AU - Wolstenholme, Jennifer AU - Russell, James T AU - Hyman, Steven E AD - Molecular Plasticity Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA. pvoul001@umaryland.edu Y1 - 2005/04/13/ PY - 2005 DA - 2005 Apr 13 SP - 3763 EP - 3773 VL - 25 IS - 15 KW - 2-chloro-5-hydroxyphenylglycine KW - 0 KW - Benzazepines KW - Benzoates KW - Dicarboxylic Acids KW - Dopamine Agonists KW - Dopamine Antagonists KW - Enzyme Inhibitors KW - Excitatory Amino Acid Agonists KW - Excitatory Amino Acid Antagonists KW - Phenylacetates KW - Receptors, Dopamine D1 KW - Receptors, Metabotropic Glutamate KW - metabotropic glutamate receptor type 1 KW - alpha-methyl-4-carboxyphenylglycine KW - 146669-29-6 KW - Methoxyhydroxyphenylglycol KW - 534-82-7 KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine KW - 67287-49-4 KW - dihydroxyphenylethylene glycol KW - CF5G2G268A KW - Protein Kinase C KW - EC 2.7.11.13 KW - Mitogen-Activated Protein Kinase 1 KW - EC 2.7.11.24 KW - Calcium KW - SY7Q814VUP KW - Glycine KW - TE7660XO1C KW - Index Medicus KW - Animals KW - Drug Interactions KW - Benzazepines -- pharmacology KW - Analysis of Variance KW - Phenylacetates -- pharmacology KW - Dopamine Antagonists -- pharmacology KW - Dicarboxylic Acids -- pharmacology KW - Methoxyhydroxyphenylglycol -- analogs & derivatives KW - Glycine -- analogs & derivatives KW - 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine -- pharmacology KW - Models, Biological KW - Excitatory Amino Acid Antagonists -- pharmacology KW - Rats KW - Calcium -- metabolism KW - Phosphorylation KW - Dopamine Agonists -- pharmacology KW - Glycine -- pharmacology KW - Excitatory Amino Acid Agonists -- pharmacology KW - Protein Kinase C -- pharmacology KW - Embryo, Mammalian KW - Fluorescent Antibody Technique KW - Benzoates -- pharmacology KW - Pregnancy KW - Rats, Sprague-Dawley KW - Blotting, Western KW - Transfection KW - Cells, Cultured KW - Enzyme Inhibitors -- pharmacology KW - Methoxyhydroxyphenylglycol -- pharmacology KW - Female KW - Corpus Striatum -- cytology KW - Neurons -- metabolism KW - Receptors, Dopamine D1 -- physiology KW - Neurons -- drug effects KW - Mitogen-Activated Protein Kinase 1 -- metabolism KW - Receptors, Metabotropic Glutamate -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67743234?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Metabotropic+glutamate+receptors+and+dopamine+receptors+cooperate+to+enhance+extracellular+signal-regulated+kinase+phosphorylation+in+striatal+neurons.&rft.au=Voulalas%2C+Pamela+J%3BHoltzclaw%2C+Lynne%3BWolstenholme%2C+Jennifer%3BRussell%2C+James+T%3BHyman%2C+Steven+E&rft.aulast=Voulalas&rft.aufirst=Pamela&rft.date=2005-04-13&rft.volume=25&rft.issue=15&rft.spage=3763&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-13 N1 - Date created - 2005-04-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Activation of orbital and medial prefrontal cortex by methylphenidate in cocaine-addicted subjects but not in controls: relevance to addiction. AN - 67743194; 15829645 AB - Drugs of abuse are rewarding to addicted and nonaddicted subjects, but they trigger craving and compulsive intake only in addicted subjects. Here, we used positron emission tomography (PET) and [18F] deoxyglucose to compare the brain metabolic responses (marker of brain function) of cocaine-addicted subjects (n = 21) and controls (n = 15) to identify brain regions that are uniquely activated in addicted subjects by intravenous methylphenidate (a drug that cocaine-addicted subjects report to be similar to cocaine). In parallel, we also measured the changes in dopamine (DA) induced by intravenous methylphenidate (using PET and [11C] raclopride) in the striatum and in the thalamus. Metabolic responses between groups differed significantly only in the right medial orbital prefrontal cortex [Brodmann's area (BA) 25 and medial BA 11], where methylphenidate increased metabolism in addicted subjects but decreased metabolism in controls. These changes were associated in all subjects with increased "desire for methylphenidate" and in the addicted subjects with "cocaine craving." In addicted subjects, increases in BA 25 were also associated with mood elevation. Methylphenidate-induced increases in metabolism in the medial orbital prefrontal cortex were associated with its increase of DA in the thalamus but not in the striatum. These findings provide evidence that enhanced sensitivity of BA 25 (region involved with emotional reactivity) and BA 11 (region involved with salience attribution and motivation) in cocaine-addicted subjects may underlie the strong emotional response to the drug and the intense desire to procure it that results in craving and compulsive drug intake. It also suggests that the mesothalamic DA pathway may contribute to these processes. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Volkow, Nora D AU - Wang, Gene-Jack AU - Ma, Yeming AU - Fowler, Joanna S AU - Wong, Christopher AU - Ding, Yu-Shin AU - Hitzemann, Robert AU - Swanson, James M AU - Kalivas, Peter AD - National Institute of Drug Abuse, Rockville, Maryland 20857, USA. nvolkow@nida.nih.gov Y1 - 2005/04/13/ PY - 2005 DA - 2005 Apr 13 SP - 3932 EP - 3939 VL - 25 IS - 15 KW - Carbon Isotopes KW - 0 KW - Central Nervous System Stimulants KW - Dopamine Antagonists KW - Fluorodeoxyglucose F18 KW - 0Z5B2CJX4D KW - Methylphenidate KW - 207ZZ9QZ49 KW - Raclopride KW - 430K3SOZ7G KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Brain Mapping KW - Fluorodeoxyglucose F18 -- pharmacokinetics KW - Positron-Emission Tomography -- methods KW - Humans KW - Adult KW - Carbon Isotopes -- pharmacokinetics KW - Dopamine -- metabolism KW - Dopamine Antagonists -- pharmacokinetics KW - Statistics as Topic KW - Raclopride -- pharmacokinetics KW - Male KW - Functional Laterality KW - Prefrontal Cortex -- diagnostic imaging KW - Methylphenidate -- administration & dosage KW - Behavior, Addictive -- chemically induced KW - Cocaine-Related Disorders -- psychology KW - Behavior, Addictive -- metabolism KW - Central Nervous System Stimulants -- administration & dosage KW - Prefrontal Cortex -- drug effects KW - Cocaine-Related Disorders -- metabolism KW - Behavior, Addictive -- diagnostic imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67743194?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Activation+of+orbital+and+medial+prefrontal+cortex+by+methylphenidate+in+cocaine-addicted+subjects+but+not+in+controls%3A+relevance+to+addiction.&rft.au=Volkow%2C+Nora+D%3BWang%2C+Gene-Jack%3BMa%2C+Yeming%3BFowler%2C+Joanna+S%3BWong%2C+Christopher%3BDing%2C+Yu-Shin%3BHitzemann%2C+Robert%3BSwanson%2C+James+M%3BKalivas%2C+Peter&rft.aulast=Volkow&rft.aufirst=Nora&rft.date=2005-04-13&rft.volume=25&rft.issue=15&rft.spage=3932&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-13 N1 - Date created - 2005-04-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Substrate specificity of the human protein phosphatase 2Cdelta, Wip1. AN - 67704863; 15807522 AB - Wip1, the wild-type p53-induced phosphatase, selectively dephosphorylates a threonine residue on p38 MAPK and mediates a negative feedback loop of the p38 MAPK-p53 signaling pathway. To identify the substrate specificity of Wip1, we prepared a recombinant human Wip1 catalytic domain (rWip1) and measured kinetic parameters for phosphopeptides containing the dephosphorylation sites in p38alpha and in a new substrate, UNG2. rWip1 showed properties that were comparable to those of PP2Calpha or full-length Wip1 in terms of affinity for Mg(2+), insensitivity to okadaic acid, and threonine dephosphorylation. The substrate specificity constant k(cat)/K(m) for a diphosphorylated peptide with a pTXpY sequence was 6-8-fold higher than that of a monophosphorylated peptide with a pTXY sequence, while PP2Calpha showed a preference for monophosphorylated peptides. Although individual side chains before and after the pTXpY sequence of the substrate did not have a significant effect on rWip1 activity, a chain length of at least five residues, including the pTXpY sequence, was important for substrate recognition by rWip1. Moreover, the X residue in the pTXpY sequence affected affinity for rWip1 and correlated with selectivity for MAPKs. These findings suggest that substrate recognition by Wip1 is centered toward a very narrow region around the pTXpY sequence. Three-dimension homology models of Wip1 with bound substrate peptides were constructed, and site-directed mutagenesis was performed to confirm the importance of specific residues for substrate recognition. The results of our study should be useful for predicting new physiological substrates and for designing specific Wip1 inhibitors. JF - Biochemistry AU - Yamaguchi, Hiroshi AU - Minopoli, Giuseppina AU - Demidov, Oleg N AU - Chatterjee, Deb K AU - Anderson, Carl W AU - Durell, Stewart R AU - Appella, Ettore AD - Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/04/12/ PY - 2005 DA - 2005 Apr 12 SP - 5285 EP - 5294 VL - 44 IS - 14 SN - 0006-2960, 0006-2960 KW - DNA Primers KW - 0 KW - Enzyme Inhibitors KW - Neoplasm Proteins KW - Okadaic Acid KW - 1W21G5Q4N2 KW - Threonine KW - 2ZD004190S KW - PPM1D protein, human KW - EC 3.1.3.16 KW - Phosphoprotein Phosphatases KW - Protein Phosphatase 2C KW - Index Medicus KW - Threonine -- metabolism KW - Humans KW - Catalytic Domain KW - Amino Acid Sequence KW - Base Sequence KW - Phosphorylation KW - Kinetics KW - Molecular Sequence Data KW - Okadaic Acid -- pharmacology KW - Enzyme Inhibitors -- pharmacology KW - Substrate Specificity KW - Sequence Homology, Amino Acid KW - Phosphoprotein Phosphatases -- antagonists & inhibitors KW - Neoplasm Proteins -- antagonists & inhibitors KW - Phosphoprotein Phosphatases -- metabolism KW - Phosphoprotein Phosphatases -- chemistry KW - Neoplasm Proteins -- chemistry KW - Neoplasm Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67704863?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Substrate+specificity+of+the+human+protein+phosphatase+2Cdelta%2C+Wip1.&rft.au=Yamaguchi%2C+Hiroshi%3BMinopoli%2C+Giuseppina%3BDemidov%2C+Oleg+N%3BChatterjee%2C+Deb+K%3BAnderson%2C+Carl+W%3BDurell%2C+Stewart+R%3BAppella%2C+Ettore&rft.aulast=Yamaguchi&rft.aufirst=Hiroshi&rft.date=2005-04-12&rft.volume=44&rft.issue=14&rft.spage=5285&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cadmium-induced malignant transformation in rat liver cells: role of aberrant oncogene expression and minimal role of oxidative stress. AN - 67430536; 15551354 AB - Our study examined the role of oxidative stress and aberrant gene expression in malignant transformation induced by chronic, low-level cadmium exposure in non-tumorigenic rat liver epithelial cell line, TRL 1215. Cells were cultured in 1.0 microM cadmium (as CdCl(2)) for up to 28 weeks and compared to passage-matched control cells. The level of cadmium used for transformation produced no evidence of increased superoxide (O(2) (-*.)) or hydrogen peroxide (H(2)O(2)) levels in the early stages of exposure ( 100 transformants/10(7) conidia). The majority of transformants harbored a randomly integrated single copy of T-DNA and were mitotically stable. We chose alb1, a polyketide synthase gene, as the target gene for homologous integration because of the clear phenotype difference between the white colonies of Deltaalb1 mutant strains and the bluish-green colonies of wild-type strains. ATMT with a T-DNA-containing alb1 disruption construct resulted in 66% albino transformants. Southern analysis revealed that 19 of the 20 randomly chosen albino transformants (95%) were disrupted by homologous recombination. These results suggest that ATMT is an efficient tool for transformation, random insertional mutagenesis, and gene disruption in A. fumigatus. JF - Applied and environmental microbiology AU - Sugui, Janyce A AU - Chang, Yun C AU - Kwon-Chung, K J AD - Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 1798 EP - 1802 VL - 71 IS - 4 SN - 0099-2240, 0099-2240 KW - Polyketide Synthases KW - 79956-01-7 KW - Index Medicus KW - Polyketide Synthases -- genetics KW - Humans KW - Mutagenesis, Insertional KW - Gene Deletion KW - Agrobacterium tumefaciens -- genetics KW - Transformation, Bacterial KW - Aspergillus fumigatus -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67708552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+and+environmental+microbiology&rft.atitle=Agrobacterium+tumefaciens-mediated+transformation+of+Aspergillus+fumigatus%3A+an+efficient+tool+for+insertional+mutagenesis+and+targeted+gene+disruption.&rft.au=Sugui%2C+Janyce+A%3BChang%2C+Yun+C%3BKwon-Chung%2C+K+J&rft.aulast=Sugui&rft.aufirst=Janyce&rft.date=2005-04-01&rft.volume=71&rft.issue=4&rft.spage=1798&rft.isbn=&rft.btitle=&rft.title=Applied+and+environmental+microbiology&rft.issn=00992240&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-06 N1 - Date created - 2005-04-06 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nat Biotechnol. 1998 Sep;16(9):839-42 [9743116] J Bacteriol. 1998 Jun;180(12):3031-8 [9620950] Nat Biotechnol. 1999 Jun;17(6):598-601 [10385327] J Bacteriol. 2001 Dec;183(23):6852-61 [11698374] Annu Rev Microbiol. 2002;56:433-55 [12142473] Microbiol Mol Biol Rev. 2003 Mar;67(1):16-37, table of contents [12626681] Infect Immun. 2003 May;71(5):2819-26 [12704156] Curr Genet. 2003 Aug;43(5):371-7 [12756496] Fungal Genet Biol. 2003 Aug;39(3):264-75 [12892639] Curr Genet. 2004 Feb;45(1):54-60 [14586554] Gene. 1987;56(1):117-24 [2824287] EMBO J. 1995 Jul 3;14(13):3206-14 [7621833] Methods Mol Biol. 1995;55:63-72 [8528423] J Bacteriol. 1996 Mar;178(6):1498-504 [8626274] Clin Infect Dis. 1998 Apr;26(4):781-803; quiz 804-5 [9564455] Med Microbiol Immunol. 1998 Oct;187(2):79-89 [9832321] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Safety and efficacy of the nicotine patch and gum for the treatment of adolescent tobacco addiction. AN - 67704168; 15805342 AB - To determine the safety and efficacy of the nicotine patch and gum for adolescents who want to quit smoking. Double-blind, double-dummy, randomized, 3-arm trial with a nicotine patch (21 mg), nicotine gum (2 and 4 mg), or a placebo patch and gum; all participants received cognitive-behavioral group therapy. Inner-city, outpatient clinic on the East Coast. Subjects. Thirteen- to 17-year-old adolescents who smoked > or =10 cigarettes per day (CPD), scored > or =5 on the Fagerstrom Test of Nicotine Dependence, and were motivated to quit smoking. Intervention. Twelve weeks of nicotine patch or gum therapy with cognitive-behavioral therapy, with a follow-up visit at 6 months (3 months after the end of treatment). Safety assessed on the basis of adverse event reports for all 3 groups, prolonged abstinence, assessed through self-report and verified with exhaled carbon monoxide (CO) levels of < or =6 ppm, in intent-to-treat analyses, and smoking reduction (CPD and thiocyanate concentrations) among trial completers. A total of 120 participants were randomized (72% white, 70% female; age: 15.2 +/- 1.33 years; smoking: 18.8 +/- 8.56 CPD; Fagerstrom Test of Nicotine Dependence score: 7.04 +/- 1.29) from 1999 to 2003. Participants started smoking at 11.2 +/- 1.98 years of age and had been smoking daily for 2.66 +/- 1.56 years; 75% had at least 1 current psychiatric diagnosis. Mean compliance across groups was higher for the patch (mean: 78.4-82.8%) than for the gum (mean: 38.5-50.7%). Both the patch and gum were well tolerated, and adverse events were similar to those reported in adult trials. Changes in mean saliva cotinine concentrations throughout treatment were not statistically significant. Intent-to-treat analyses of all randomized participants showed CO-confirmed prolonged abstinence rates of 18% for the active-patch group, 6.5% for the active-gum group, and 2.5% for the placebo group; the difference between the active-patch and placebo arms was statistically significant. There was no significant effect of patch versus gum or gum versus placebo on cessation outcomes. Abstinence rates at the 3-month follow-up assessment were sustained but were not significantly associated with treatment group. Mean smoking rates, but not CO or thiocyanate concentrations, decreased significantly in all 3 arms but not as a function of treatment group. Nicotine patch therapy combined with cognitive-behavioral intervention was effective, compared with placebo, for treatment of tobacco dependence among adolescent smokers. Decreases in the numbers of cigarettes smoked appeared to be offset by compensatory smoking. Additional study of nicotine gum, with enhanced instructional support, is needed to assess its efficacy among adolescent smokers. JF - Pediatrics AU - Moolchan, Eric T AU - Robinson, Miqun L AU - Ernst, Monique AU - Cadet, Jean Lud AU - Pickworth, Wallace B AU - Heishman, Stephen J AU - Schroeder, Jennifer R AD - Teen Tobacco Addiction Research Clinic, Clinical Pharmacology and Therapeutics Research Branch, National Institute on Drug Abuse, Intramural Research Program, 5500 Nathan Shock Dr, Baltimore, Maryland 21224, USA. emoolcha@intra.nida.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - e407 EP - e414 VL - 115 IS - 4 KW - Chewing Gum KW - 0 KW - Nicotinic Agonists KW - Nicotine KW - 6M3C89ZY6R KW - Abridged Index Medicus KW - Index Medicus KW - Administration, Cutaneous KW - Double-Blind Method KW - Logistic Models KW - Humans KW - Adolescent KW - Male KW - Female KW - Nicotine -- therapeutic use KW - Nicotinic Agonists -- therapeutic use KW - Chewing Gum -- adverse effects KW - Tobacco Use Disorder -- drug therapy KW - Nicotine -- adverse effects KW - Smoking Cessation -- methods KW - Nicotinic Agonists -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67704168?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatrics&rft.atitle=Safety+and+efficacy+of+the+nicotine+patch+and+gum+for+the+treatment+of+adolescent+tobacco+addiction.&rft.au=Moolchan%2C+Eric+T%3BRobinson%2C+Miqun+L%3BErnst%2C+Monique%3BCadet%2C+Jean+Lud%3BPickworth%2C+Wallace+B%3BHeishman%2C+Stephen+J%3BSchroeder%2C+Jennifer+R&rft.aulast=Moolchan&rft.aufirst=Eric&rft.date=2005-04-01&rft.volume=115&rft.issue=4&rft.spage=e407&rft.isbn=&rft.btitle=&rft.title=Pediatrics&rft.issn=1098-4275&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-11 N1 - Date created - 2005-04-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effects on alcohol related fatal crashes of a community based initiative to increase substance abuse treatment and reduce alcohol availability. AN - 67699911; 15805436 AB - This analysis tested whether comprehensive community interventions that focus on reducing alcohol availability and increasing substance abuse treatment can reduce alcohol related fatal traffic crashes. Five of 14 communities awarded Fighting Back grants by The Robert Wood Johnson Foundation to reduce substance abuse and related problems attempted to reduce availability of alcohol and expand substance abuse treatment programs (FBAT communities). Program implementation began on 1 January 1992. A quasi-experimental design matched each program community to two or three other communities of similar demographic composition in the same state. The ratio of fatal crashes involving a driver or pedestrian with a blood alcohol concentration of 0.01% or higher, 0.08% or higher, or 0.15% or higher were examined relative to fatal crashes where no alcohol was involved for 10 years preceding and 10 years following program initiation. Relative to their comparison communities, the five FBAT communities experienced significant declines of 22% in alcohol related fatal crashes at 0.01% BAC or higher, 20% at 0.08% or higher, and 17% at 0.15% or higher relative to fatal crashes not involving alcohol. Community interventions to reduce alcohol availability and increase substance abuse treatment can reduce alcohol related fatal traffic crashes. JF - Injury prevention : journal of the International Society for Child and Adolescent Injury Prevention AU - Hingson, R W AU - Zakocs, R C AU - Heeren, T AU - Winter, M R AU - Rosenbloom, D AU - DeJong, W AD - Boston University School of Public Health, Center to Prevent Alcohol-related Problems Among Young People, Boston, MA 02118, USA. rhingson@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 84 EP - 90 VL - 11 IS - 2 SN - 1353-8047, 1353-8047 KW - Ethanol KW - 3K9958V90M KW - Index Medicus KW - United States KW - Ethanol -- blood KW - Program Evaluation -- methods KW - Humans KW - Alcoholic Beverages -- supply & distribution KW - Automobile Driving KW - Accidents, Traffic -- mortality KW - Community Health Services -- methods KW - Accidents, Traffic -- trends KW - Consumer Advocacy KW - Alcohol-Related Disorders -- prevention & control KW - Alcohol Drinking -- prevention & control KW - Accidents, Traffic -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67699911?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Injury+prevention+%3A+journal+of+the+International+Society+for+Child+and+Adolescent+Injury+Prevention&rft.atitle=Effects+on+alcohol+related+fatal+crashes+of+a+community+based+initiative+to+increase+substance+abuse+treatment+and+reduce+alcohol+availability.&rft.au=Hingson%2C+R+W%3BZakocs%2C+R+C%3BHeeren%2C+T%3BWinter%2C+M+R%3BRosenbloom%2C+D%3BDeJong%2C+W&rft.aulast=Hingson&rft.aufirst=R&rft.date=2005-04-01&rft.volume=11&rft.issue=2&rft.spage=84&rft.isbn=&rft.btitle=&rft.title=Injury+prevention+%3A+journal+of+the+International+Society+for+Child+and+Adolescent+Injury+Prevention&rft.issn=13538047&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-04-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Consult Clin Psychol. 1999 Dec;67(6):989-94 [10596521] Alcohol Alcohol. 1999 Jul-Aug;34(4):609-21 [10456590] Inj Prev. 2000 Jun;6(2):109-14 [10875666] JAMA. 2000 Nov 8;284(18):2341-7 [11066184] Am J Prev Med. 2001 Nov;21(4 Suppl):66-88 [11691562] J Stud Alcohol. 2001 Nov;62(6):806-16 [11838918] J Stud Alcohol Suppl. 2002 Mar;(14):226-40 [12022727] Ann Intern Med. 2004 Apr 6;140(7):557-68 [15068985] Traffic Inj Prev. 2004 Sep;5(3):220-7 [15276922] Traffic Inj Prev. 2004 Sep;5(3):278-91 [15276929] Alcohol Res Health. 2003;27(1):63-78 [15301401] J Pediatr. 2004 Sep;145(3):396-402 [15343198] Lancet. 2004 Oct 9-15;364(9442):1334-9 [15474136] Control Clin Trials. 1986 Sep;7(3):177-88 [3802833] Issues Sci Technol. 1991 Summer;7(4):78-84 [10170798] Bull N Y Acad Med. 1994 Summer;71(1):111-35 [8069272] Addiction. 1995 Jul;90(7):907-26 [7663313] Am J Public Health. 1996 Jun;86(6):791-7 [8659651] Addiction. 2000 Feb;95(2):209-17 [10723849] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - BLM helicase facilitates Mus81 endonuclease activity in human cells. AN - 67698358; 15805243 AB - Bloom syndrome is a rare, autosomal recessive inherited disorder in humans. The product of the Bloom syndrome mutated gene, designated BLM, is a member of the RecQ helicase family. BLM has been proposed to function at the interface of replication and recombination, and to facilitate the repair of DNA damage. Here, we report in vivo physical interaction and colocalization of BLM and a DNA structure-specific endonuclease, Mus81, at sites of stalled replication forks outside the promyelocytic leukemia nuclear bodies during the S-phase arrest of the cell cycle. Amino acids 125 to 244 of Mus81 interact with the C-terminal region (amino acids 1,007-1,417) of BLM. Whereas Mus81 does not have any effect on the helicase activity of BLM, BLM can stimulate Mus81 endonuclease activity on the nicked Holliday junctions and 3' flap. This stimulation is due to enhanced binding of Mus81 to the DNA substrates. These data suggest a new function of BLM in cooperating with Mus81 during processing and restoration of stalled replication forks. JF - Cancer research AU - Zhang, Ran AU - Sengupta, Sagar AU - Yang, Qin AU - Linke, Steven P AU - Yanaihara, Nozomu AU - Bradsher, John AU - Blais, Veronique AU - McGowan, Clare H AU - Harris, Curtis C AD - Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USA. Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 2526 EP - 2531 VL - 65 IS - 7 SN - 0008-5472, 0008-5472 KW - DNA-Binding Proteins KW - 0 KW - DNA KW - 9007-49-2 KW - Endonucleases KW - EC 3.1.- KW - MUS81 protein, human KW - Adenosine Triphosphatases KW - EC 3.6.1.- KW - Bloom syndrome protein KW - DNA Helicases KW - EC 3.6.4.- KW - RecQ Helicases KW - EC 3.6.4.12 KW - Index Medicus KW - Fibroblasts -- enzymology KW - Peptide Mapping KW - Transfection KW - Humans KW - DNA -- metabolism KW - DNA Replication -- physiology KW - HCT116 Cells KW - DNA -- biosynthesis KW - Cell Line KW - Binding Sites KW - Endonucleases -- metabolism KW - DNA Helicases -- metabolism KW - DNA-Binding Proteins -- genetics KW - DNA Helicases -- genetics KW - Adenosine Triphosphatases -- metabolism KW - Endonucleases -- genetics KW - Adenosine Triphosphatases -- genetics KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67698358?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=BLM+helicase+facilitates+Mus81+endonuclease+activity+in+human+cells.&rft.au=Zhang%2C+Ran%3BSengupta%2C+Sagar%3BYang%2C+Qin%3BLinke%2C+Steven+P%3BYanaihara%2C+Nozomu%3BBradsher%2C+John%3BBlais%2C+Veronique%3BMcGowan%2C+Clare+H%3BHarris%2C+Curtis+C&rft.aulast=Zhang&rft.aufirst=Ran&rft.date=2005-04-01&rft.volume=65&rft.issue=7&rft.spage=2526&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-26 N1 - Date created - 2005-04-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Risk of new cancers after radiotherapy in long-term survivors of retinoblastoma: an extended follow-up. AN - 67569412; 15800318 AB - Many children diagnosed with retinoblastoma (Rb) survive into adulthood and are prone to subsequent cancers, particularly hereditary patients, who have germline Rb-1 mutations. We have extended the follow-up of a large cohort of Rb patients for 7 more years to provide new information on the risk of additional cancers after radiotherapy in long-term survivors. We analyzed the risk of new cancers through 2000 in 1,601 Rb survivors, diagnosed from 1914 to 1984, at two US medical centers. The standardized incidence ratio (SIR) was calculated as the ratio of the observed number of cancers after hereditary and nonhereditary Rb to the expected number from the Connecticut Tumor Registry. The cumulative incidence of a new cancer after hereditary and nonhereditary Rb and radiotherapy was calculated with adjustment for competing risk of death. Subsequent cancer risk in 963 hereditary patients (SIR, 19; 95% CI, 16 to 21) exceeded the risk in 638 nonhereditary Rb patients (SIR, 1.2; 95% CI, 0.7 to 2.0). Radiation further increased the risk of another cancer in hereditary patients by 3.1-fold (95% CI, 2.0 to 5.3). Hereditary patients continued to be at significantly increased risk for sarcomas, melanoma, and cancers of the brain and nasal cavities. The cumulative incidence for developing a new cancer at 50 years after diagnosis of Rb was 36% (95% CI, 31% to 41%) for hereditary and 5.7% (95% CI, 2.4% to 11%) for nonhereditary patients. Hereditary Rb predisposes to a variety of new cancers over time, with radiotherapy further enhancing the risk of tumors arising in the radiation field. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Kleinerman, Ruth A AU - Tucker, Margaret A AU - Tarone, Robert E AU - Abramson, David H AU - Seddon, Johanna M AU - Stovall, Marilyn AU - Li, Frederick P AU - Fraumeni, Joseph F AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Rockville, MD 20852-7362, USA. Kleinerr@mail.nih.gov Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 2272 EP - 2279 VL - 23 IS - 10 SN - 0732-183X, 0732-183X KW - Index Medicus KW - Nasal Cavity KW - Skin Neoplasms -- etiology KW - Brachytherapy -- adverse effects KW - Humans KW - Melanoma -- etiology KW - Child KW - Child, Preschool KW - Infant KW - Nose Neoplasms -- etiology KW - Incidence KW - Follow-Up Studies KW - Sarcoma -- etiology KW - Female KW - Male KW - Retinal Neoplasms -- genetics KW - Neoplasms, Radiation-Induced -- etiology KW - Registries -- statistics & numerical data KW - Retinoblastoma -- genetics KW - Retinal Neoplasms -- radiotherapy KW - Retinoblastoma -- radiotherapy KW - Genetic Predisposition to Disease KW - Survivors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67569412?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Risk+of+new+cancers+after+radiotherapy+in+long-term+survivors+of+retinoblastoma%3A+an+extended+follow-up.&rft.au=Kleinerman%2C+Ruth+A%3BTucker%2C+Margaret+A%3BTarone%2C+Robert+E%3BAbramson%2C+David+H%3BSeddon%2C+Johanna+M%3BStovall%2C+Marilyn%3BLi%2C+Frederick+P%3BFraumeni%2C+Joseph+F&rft.aulast=Kleinerman&rft.aufirst=Ruth&rft.date=2005-04-01&rft.volume=23&rft.issue=10&rft.spage=2272&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-21 N1 - Date created - 2005-03-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with refractory metastatic melanoma. AN - 67567850; 15800326 AB - We investigated the combination of lymphodepleting chemotherapy followed by the adoptive transfer of autologous tumor reactive lymphocytes for the treatment of patients with refractory metastatic melanoma. Thirty-five patients with metastatic melanoma, all but one with disease refractory to treatment with high-dose interleukin (IL) -2 and many with progressive disease after chemotherapy, underwent lymphodepleting conditioning with two days of cyclophosphamide (60 mg/kg) followed by five days of fludarabine (25 mg/m(2)). On the day following the final dose of fludarabine, all patients received cell infusion with autologous tumor-reactive, rapidly expanded tumor infiltrating lymphocyte cultures and high-dose IL-2 therapy. Eighteen (51%) of 35 treated patients experienced objective clinical responses including three ongoing complete responses and 15 partial responses with a mean duration of 11.5 +/- 2.2 months. Sites of regression included metastases to lung, liver, lymph nodes, brain, and cutaneous and subcutaneous tissues. Toxicities of treatment included the expected hematologic toxicities of chemotherapy including neutropenia, thrombocytopenia, and lymphopenia, the transient toxicities of high-dose IL-2 therapy, two patients who developed Pneumocystis pneumonia and one patient who developed an Epstein-Barr virus-related lymphoproliferation. Lymphodepleting chemotherapy followed by the transfer of highly avid antitumor lymphocytes can mediate significant tumor regression in heavily pretreated patients with IL-2 refractory metastatic melanoma. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Dudley, Mark E AU - Wunderlich, John R AU - Yang, James C AU - Sherry, Richard M AU - Topalian, Suzanne L AU - Restifo, Nicholas P AU - Royal, Richard E AU - Kammula, Udai AU - White, Don E AU - Mavroukakis, Sharon A AU - Rogers, Linda J AU - Gracia, Gerald J AU - Jones, Stephanie A AU - Mangiameli, David P AU - Pelletier, Michelle M AU - Gea-Banacloche, Juan AU - Robinson, Michael R AU - Berman, David M AU - Filie, Armando C AU - Abati, Andrea AU - Rosenberg, Steven A AD - Surgery Branch, National Cancer Institute, NIH, CRC 3-3940, 10 Center Dr MSC 1201, Bethesda MD 20892-1202, USA. Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 2346 EP - 2357 VL - 23 IS - 10 SN - 0732-183X, 0732-183X KW - Interleukin-2 KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Vidarabine KW - FA2DM6879K KW - fludarabine KW - P2K93U8740 KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Interleukin-2 -- pharmacology KW - Humans KW - Disease Progression KW - Aged KW - Child KW - Drug Resistance, Neoplasm KW - Adult KW - Treatment Outcome KW - Neoplasm Metastasis KW - Middle Aged KW - Adolescent KW - Female KW - Male KW - Vidarabine -- analogs & derivatives KW - Adoptive Transfer KW - Skin Neoplasms -- immunology KW - Skin Neoplasms -- therapy KW - Melanoma -- therapy KW - Vidarabine -- administration & dosage KW - Melanoma -- immunology KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Lymphocytes, Tumor-Infiltrating UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67567850?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Adoptive+cell+transfer+therapy+following+non-myeloablative+but+lymphodepleting+chemotherapy+for+the+treatment+of+patients+with+refractory+metastatic+melanoma.&rft.au=Dudley%2C+Mark+E%3BWunderlich%2C+John+R%3BYang%2C+James+C%3BSherry%2C+Richard+M%3BTopalian%2C+Suzanne+L%3BRestifo%2C+Nicholas+P%3BRoyal%2C+Richard+E%3BKammula%2C+Udai%3BWhite%2C+Don+E%3BMavroukakis%2C+Sharon+A%3BRogers%2C+Linda+J%3BGracia%2C+Gerald+J%3BJones%2C+Stephanie+A%3BMangiameli%2C+David+P%3BPelletier%2C+Michelle+M%3BGea-Banacloche%2C+Juan%3BRobinson%2C+Michael+R%3BBerman%2C+David+M%3BFilie%2C+Armando+C%3BAbati%2C+Andrea%3BRosenberg%2C+Steven+A&rft.aulast=Dudley&rft.aufirst=Mark&rft.date=2005-04-01&rft.volume=23&rft.issue=10&rft.spage=2346&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-21 N1 - Date created - 2005-03-31 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Immunother. 2003 Sep-Oct;26(5):385-93 [12973027] Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1969-74 [14762166] J Immunol. 2004 May 15;172(10):6057-64 [15128789] J Immunol. 1980 Aug;125(2):711-4 [6156213] J Exp Med. 1982 Apr 1;155(4):1063-74 [6460831] J Exp Med. 1982 Aug 1;156(2):385-97 [6980254] Science. 1986 Sep 19;233(4770):1318-21 [3489291] J Immunol Methods. 1990 Apr 17;128(2):189-201 [1691237] J Exp Med. 1994 Jul 1;180(1):347-52 [7516411] J Natl Cancer Inst. 1994 Aug 3;86(15):1159-66 [8028037] J Immunol. 1995 Apr 15;154(8):3961-8 [7706734] Int J Cancer. 1996 Feb 8;65(4):413-21 [8621219] Int J Cancer. 1998 Feb 9;75(4):517-24 [9466650] Immunol Rev. 1997 Dec;160:91-102 [9476668] J Exp Med. 1998 Jul 20;188(2):277-86 [9670040] Science. 2002 Oct 25;298(5594):850-4 [12242449] Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2982-7 [10077623] Cancer. 1999 Feb 25;87(1):37-42 [10096358] J Exp Med. 2003 Aug 18;198(4):569-80 [12925674] Nat Rev Cancer. 2003 Sep;3(9):666-75 [12951585] J Immunol. 2005 Mar 1;174(5):2591-601 [15728465] Eur J Immunol. 2003 Aug;33(8):2123-32 [12884286] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8372-7 [12826605] J Immunother. 2003 Jul-Aug;26(4):332-42 [12843795] Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16168-73 [12427970] Blood. 2002 Dec 1;100(12):3877-86 [12433695] Blood. 2002 Oct 1;100(7):2562-71 [12239170] J Immunother. 2002 May-Jun;25(3):243-51 [12000866] J Immunother. 2001 Jul-Aug;24(4):363-73 [11565838] J Clin Oncol. 2001 Aug 1;19(15):3477-82 [11481353] Cancer J Sci Am. 2000 Feb;6 Suppl 1:S11-4 [10685652] Blood. 2000 Apr 1;95(7):2269-74 [10733495] N Engl J Med. 2000 Sep 14;343(11):750-8 [10984562] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The mitotic chromosome binding activity of the papillomavirus E2 protein correlates with interaction with the cellular chromosomal protein, Brd4. AN - 67564681; 15795266 AB - The papillomavirus transcriptional activator, E2, is involved in key functions of the viral life cycle. These include transcriptional regulation, viral DNA replication, and viral genome segregation. The transactivation domain of E2 is required for each of these functions. To identify the regions of the domain that mediate binding to mitotic chromosomes, a panel of mutations has been generated and their effect on various E2 functions has been analyzed. A structural model of the bovine papillomavirus type 1 (BPV1) E2 transactivation domain was generated based on its homology with the solved structure of the human papillomavirus type 16 (HPV16) domain. This model was used to identify distinct surfaces of the domain to be targeted by point mutation to further delineate the functional region of the transactivation domain responsible for mitotic chromosome association. The mutated E2 proteins were assessed for mitotic chromosome binding and, in addition, transcriptional activation and transcriptional repression activities. Mutation of amino acids R37 and I73, which are located on a surface of the domain that in HPV16 E2 is reported to mediate self-interaction, completely eliminated mitotic chromosome binding. Mitotic chromosome binding activity was found to correlate well with the ability to interact with the cellular chromosomal associated factor Brd4, which has recently been proposed to mediate the association between BPV1 E2 and mitotic chromosomes. JF - Journal of virology AU - Baxter, Michael K AU - McPhillips, Maria G AU - Ozato, Keiko AU - McBride, Alison A AD - Laboratory of Viral Diseases, NIAID, NIH, Building 4, Room 137, 4 Center Dr., MSC 0455, Bethesda, MD 20892-0455, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 4806 EP - 4818 VL - 79 IS - 8 SN - 0022-538X, 0022-538X KW - BRD4 protein, human KW - 0 KW - DNA-Binding Proteins KW - E2 protein, Bovine papillomavirus KW - Nuclear Proteins KW - Oncogene Proteins, Fusion KW - Oncogene Proteins, Viral KW - Recombinant Proteins KW - Transcription Factors KW - Viral Proteins KW - oncogene protein E2, Human papillomavirus type 1 KW - Index Medicus KW - Animals KW - HeLa Cells KW - Models, Molecular KW - Humans KW - Viral Proteins -- chemistry KW - Transcriptional Activation KW - Mutagenesis, Site-Directed KW - Recombinant Proteins -- metabolism KW - Oncogene Proteins, Fusion -- chemistry KW - Oncogene Proteins, Fusion -- genetics KW - Viral Proteins -- metabolism KW - Oncogene Proteins, Fusion -- metabolism KW - Chromosomes, Human -- genetics KW - DNA Replication KW - Protein Conformation KW - Cell Division KW - DNA-Binding Proteins -- chemistry KW - Oncogene Proteins, Viral -- chemistry KW - Mitosis -- genetics KW - DNA-Binding Proteins -- genetics KW - Oncogene Proteins, Viral -- genetics KW - Oncogene Proteins, Viral -- metabolism KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67564681?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=The+mitotic+chromosome+binding+activity+of+the+papillomavirus+E2+protein+correlates+with+interaction+with+the+cellular+chromosomal+protein%2C+Brd4.&rft.au=Baxter%2C+Michael+K%3BMcPhillips%2C+Maria+G%3BOzato%2C+Keiko%3BMcBride%2C+Alison+A&rft.aulast=Baxter&rft.aufirst=Michael&rft.date=2005-04-01&rft.volume=79&rft.issue=8&rft.spage=4806&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-17 N1 - Date created - 2005-03-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Gen Virol. 2002 Jan;83(Pt 1):179-88 [11752715] Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2998-3003 [15710895] Mol Cell Biol. 2002 Sep;22(18):6509-20 [12192049] J Virol. 2002 Nov;76(21):10914-20 [12368334] J Virol. 2003 Jun;77(12):6946-56 [12768013] Cell Mol Life Sci. 2003 Apr;60(4):721-41 [12785719] Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8758-63 [12840145] J Virol. 2004 Feb;78(4):2100-13 [14747575] J Biol Chem. 2004 Feb 20;279(8):6976-85 [14634007] Cell. 2004 Apr 30;117(3):349-60 [15109495] Genes Dev. 2004 Aug 15;18(16):1981-96 [15289463] Cell. 1985 Aug;42(1):183-91 [2990724] Nature. 1987 Dec 17-23;330(6149):670-2 [3317067] EMBO J. 1987 Nov;6(11):3391-7 [2828029] Acta Med Okayama. 1988 Aug;42(4):243-5 [2845712] EMBO J. 1988 Dec 1;7(12):3807-16 [2850174] Proc Natl Acad Sci U S A. 1989 Jan;86(2):510-4 [2536165] Genes Dev. 1989 Jan;3(1):38-48 [2540059] J Virol. 1989 Dec;63(12):5076-85 [2555544] EMBO J. 1991 Feb;10(2):449-57 [1846806] Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3204-8 [1849647] J Virol. 1991 Oct;65(10):5314-22 [1654443] EMBO J. 1991 Dec;10(13):4321-9 [1661672] Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5086-90 [8389467] J Virol. 1993 Jul;67(7):3720-9 [8389903] Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7051-5 [8041744] J Virol. 1995 Oct;69(10):6199-208 [7666521] J Virol. 1995 Dec;69(12):7791-9 [7494290] J Virol. 1996 Jan;70(1):23-9 [8523530] J Biol Chem. 1995 Dec 29;270(52):30914-8 [8537346] EMBO J. 1996 Jan 2;15(1):1-11 [8598191] J Virol. 1996 Mar;70(3):1602-11 [8627680] J Virol. 1996 Jul;70(7):4193-9 [8676438] Virology. 1996 Jul 1;221(1):34-43 [8661412] Virology. 1996 Jul 1;221(1):44-53 [8661413] J Virol. 2002 Nov;76(21):11042-53 [12368347] J Virol. 2002 Nov;76(22):11596-604 [12388720] J Virol. 1999 Dec;73(12):9789-95 [10559289] Nature. 2000 Feb 17;403(6771):805-9 [10693813] J Virol. 2000 Apr;74(8):3752-60 [10729150] Virology. 2000 Apr 25;270(1):124-34 [10772985] J Virol. 2000 Jul;74(13):5872-9 [10846067] Mol Cell Biol. 2000 Sep;20(17):6537-49 [10938129] Virology. 2000 Oct 25;276(2):304-14 [11040122] Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12513-8 [11070078] EMBO J. 2001 Jan 15;20(1-2):222-30 [11226172] Mol Cell Biol. 2001 May;21(10):3576-88 [11313483] Front Biosci. 2001 Aug 1;6:D1008-18 [11487468] J Virol. 1996 Sep;70(9):6169-79 [8709243] J Biol Chem. 1997 Nov 21;272(47):29873-9 [9368061] J Virol. 1998 Mar;72(3):2079-88 [9499063] Virology. 1998 Feb 15;241(2):312-22 [9499806] Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4338-43 [9539738] J Virol. 1998 May;72(5):3925-34 [9557678] J Mol Biol. 1998 Jul 3;280(1):1-9 [9653027] J Virol. 1999 Apr;73(4):2587-95 [10074103] J Virol. 1999 May;73(5):4385-92 [10196336] J Virol. 1999 May;73(5):4404-12 [10196338] Mol Cell Biol. 1999 May;19(5):3349-59 [10207059] J Virol. 2005 Feb;79(3):1500-9 [15650176] Virology. 2005 Feb 5;332(1):78-88 [15661142] J Virol. 2002 Mar;76(5):2480-90 [11836426] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Convection perfusion of glucocerebrosidase for neuronopathic Gaucher's disease. AN - 67557889; 15786474 AB - Systemic enzyme replacement for Gaucher's disease has not prevented premature death or severe morbidity in patients with a neuronopathic phenotype, because the enzyme does not cross the blood-brain barrier. We used convection-enhanced delivery for regional distribution of glucocerebrosidase in rat and primate brains and examined its safety and feasibility for neuronopathic Gaucher's disease. Rats underwent intrastriatal infusion and were observed and then sacrificed at 14 hours, 4 days, or 6 weeks. Primates underwent serial magnetic resonance imaging during enzyme perfusion of the right frontal lobe or brainstem, were observed and then sacrificed after infusion completion. Animals underwent histologic and enzymatic tissue analyses. Magnetic resonance imaging revealed perfusion of the primate right frontal lobe or pons with infusate. Enzyme activity was substantially and significantly (p < 0.05) increased in cortex and white matter of the infused frontal lobe and pons compared to control. Immunohistochemistry demonstrated intraneuronal glucocerebrosidase. There was no toxicity. Convection-enhanced delivery can be used to safely perfuse large regions of the brain and brainstem with therapeutic levels of glucocerebrosidase. Patients with neuronopathic Gaucher's disease and similar central nervous system disorders may benefit from this treatment. JF - Annals of neurology AU - Lonser, Russell R AU - Walbridge, Stuart AU - Murray, Gary J AU - Aizenberg, Michele R AU - Vortmeyer, Alexander O AU - Aerts, Johannes M F G AU - Brady, Roscoe O AU - Oldfield, Edward H AD - Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA. lonserr@ninds.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 542 EP - 548 VL - 57 IS - 4 SN - 0364-5134, 0364-5134 KW - Glucosylceramidase KW - EC 3.2.1.45 KW - Index Medicus KW - Rats KW - Magnetic Resonance Imaging KW - Animals KW - Perfusion -- methods KW - Macaca mulatta KW - Immunohistochemistry KW - Male KW - Convection KW - Brain -- enzymology KW - Glucosylceramidase -- administration & dosage KW - Glucosylceramidase -- metabolism KW - Gaucher Disease -- drug therapy KW - Drug Delivery Systems -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67557889?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+neurology&rft.atitle=Convection+perfusion+of+glucocerebrosidase+for+neuronopathic+Gaucher%27s+disease.&rft.au=Lonser%2C+Russell+R%3BWalbridge%2C+Stuart%3BMurray%2C+Gary+J%3BAizenberg%2C+Michele+R%3BVortmeyer%2C+Alexander+O%3BAerts%2C+Johannes+M+F+G%3BBrady%2C+Roscoe+O%3BOldfield%2C+Edward+H&rft.aulast=Lonser&rft.aufirst=Russell&rft.date=2005-04-01&rft.volume=57&rft.issue=4&rft.spage=542&rft.isbn=&rft.btitle=&rft.title=Annals+of+neurology&rft.issn=03645134&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-20 N1 - Date created - 2005-03-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Secondary structure and gating rearrangements of transmembrane segments in rat P2X4 receptor channels. AN - 67556651; 15795310 AB - P2X receptors are cation selective channels that are activated by extracellular nucleotides. These channels are likely formed by three identical or related subunits, each having two transmembrane segments (TM1 and TM2). To identify regions that undergo rearrangement during gating and to probe their secondary structure, we performed tryptophan scanning mutagenesis on the two putative TMs of the rat P2X4 receptor channel. Mutant channels were expressed in Xenopus oocytes, concentration-response relationships constructed for ATP, and the EC50 estimated by fitting the Hill equation to the data. Of the 22 mutations in TM1 and 24 in TM2, all but one in TM1 and seven in TM2 result in functional channels. Interestingly, the majority of the functional mutants display an increased sensitivity to ATP, and in general these perturbations are more pronounced for TM2 when compared with TM1. For TM1 and for the outer half of TM2, the perturbations are consistent with these regions adopting alpha-helical secondary structures. In addition, the greatest perturbations in the gating equilibrium occur for mutations near the outer ends of both TM1 and TM2. Surface biotinylation experiments reveal that all the nonfunctional mutants traffic to the surface membrane at levels comparable to the WT channel, suggesting that these mutations likely disrupt ion conduction or gating. Taken together, these results suggest that the outer parts of TM1 and TM2 are helical and that they move during activation. The observation that the majority of nonconducting mutations are clustered toward the inner end of TM2 suggests a critical functional role for this region. JF - The Journal of general physiology AU - Silberberg, Shai D AU - Chang, Tsg-Hui AU - Swartz, Kenton J AD - Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. silberbs@ninds.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 347 EP - 359 VL - 125 IS - 4 SN - 0022-1295, 0022-1295 KW - P2rx4 protein, rat KW - 0 KW - Receptors, Purinergic P2 KW - Receptors, Purinergic P2X4 KW - Index Medicus KW - Rats KW - Mutagenesis, Site-Directed KW - Xenopus laevis KW - Animals KW - Protein Structure, Secondary KW - Molecular Sequence Data KW - Oocytes -- physiology KW - Amino Acid Sequence KW - Amino Acid Substitution KW - Structure-Activity Relationship KW - Ion Channel Gating -- physiology KW - Models, Molecular KW - Receptors, Purinergic P2 -- chemistry KW - Cell Membrane -- chemistry KW - Models, Chemical KW - Cell Membrane -- physiology KW - Models, Biological KW - Receptors, Purinergic P2 -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67556651?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+general+physiology&rft.atitle=Secondary+structure+and+gating+rearrangements+of+transmembrane+segments+in+rat+P2X4+receptor+channels.&rft.au=Silberberg%2C+Shai+D%3BChang%2C+Tsg-Hui%3BSwartz%2C+Kenton+J&rft.aulast=Silberberg&rft.aufirst=Shai&rft.date=2005-04-01&rft.volume=125&rft.issue=4&rft.spage=347&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+general+physiology&rft.issn=00221295&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-03-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 2001 May 4;276(18):14902-8 [11278888] J Neurosci. 2001 Aug 15;21(16):5885-92 [11487611] J Biol Chem. 2001 Aug 17;276(33):30934-41 [11402044] J Biol Chem. 2001 Aug 31;276(35):32793-8 [11438537] Neuron. 2001 Nov 20;32(4):649-56 [11719205] Nature. 2002 Jan 17;415(6869):287-94 [11796999] Mol Pharmacol. 2002 Feb;61(2):303-11 [11809854] Annu Rev Biophys Biomol Struct. 2002;31:207-33 [11988468] J Gen Physiol. 2002 Jun;119(6):521-32 [12034760] J Gen Physiol. 2002 Jun;119(6):581-91 [12034765] Nature. 2002 May 30;417(6888):515-22 [12037559] Nature. 2002 May 30;417(6888):523-6 [12037560] Physiol Rev. 2002 Oct;82(4):1013-67 [12270951] Neuropharmacology. 2002 Sep;43(4):669-78 [12367612] Nat Neurosci. 1999 Apr;2(4):322-30 [10204538] Mol Pharmacol. 1999 Nov;56(5):973-81 [10531403] Cell. 2002 Oct 18;111(2):231-9 [12408867] Neuron. 2003 Apr 10;38(1):61-7 [12691664] Nature. 2003 May 1;423(6935):33-41 [12721618] Science. 2003 Jun 20;300(5627):1922-6 [12738871] Sci STKE. 2003 Jun 24;2003(188):re10 [12824476] Nature. 2003 Jun 26;423(6943):949-55 [12827192] J Neurosci. 2003 Oct 1;23(26):8903-10 [14523092] Biochemistry. 2003 Oct 28;42(42):12243-50 [14567686] J Gen Physiol. 2003 Nov;122(5):541-56 [14557403] J Gen Physiol. 2004 Mar;123(3):281-93 [14769846] J Biol Chem. 2004 Mar 5;279(10):9043-55 [14699168] J Neurosci. 2004 Mar 31;24(13):3413-20 [15056721] Nature. 2004 Apr 22;428(6985):864-8 [15103379] Nat Struct Mol Biol. 2004 Jun;11(6):499-501 [15164005] Trends Neurosci. 2004 Jun;27(6):321-8 [15165736] Trends Neurosci. 2004 Jun;27(6):329-36 [15165737] J Mol Biol. 2004 Sep 3;342(1):333-43 [15313628] J Neurosci. 2004 Aug 18;24(33):7378-86 [15317863] J Gen Physiol. 2004 Oct;124(4):319-32 [15365093] J Mol Biol. 1987 Jun 5;195(3):659-85 [3656427] Proc Natl Acad Sci U S A. 1988 Dec;85(23):9012-6 [3057498] Science. 1989 Aug 4;245(4917):510-3 [2667138] Annu Rev Biochem. 1989;58:607-33 [2673018] Neuron. 1992 Nov;9(5):861-71 [1419000] Nature. 1994 Oct 6;371(6497):516-9 [7523951] Nature. 1994 Oct 6;371(6497):519-23 [7523952] Neuron. 1995 Jun;14(6):1293-301 [7605638] Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12046-9 [8618841] Biophys J. 1997 Apr;72(4):1489-500 [9083655] Proc Natl Acad Sci U S A. 1997 May 13;94(10):5456-60 [9144259] Nature. 1997 Jun 5;387(6633):624-7 [9177353] EMBO J. 1997 Jun 16;16(12):3446-54 [9218787] J Neurosci. 1998 Apr 1;18(7):2350-9 [9502796] Biochem Biophys Res Commun. 1998 Mar 17;244(2):599-603 [9514958] J Gen Physiol. 1999 Mar;113(3):415-23 [10051517] Nat Neurosci. 1999 Apr;2(4):315-21 [10204537] J Gen Physiol. 2000 Jan;115(1):33-50 [10613917] J Gen Physiol. 2000 Jan;115(1):51-8 [10613918] Neuron. 2000 Feb;25(2):411-23 [10719895] Biochemistry. 2000 Apr 25;39(16):4666-73 [10769122] J Biol Chem. 2000 Sep 22;275(38):29361-7 [10827197] J Biol Chem. 2000 Nov 3;275(44):34190-6 [10940304] J Gen Physiol. 2001 Mar;117(3):205-18 [11222625] J Gen Physiol. 2001 Apr;117(4):345-60 [11279254] Neuron. 2001 Mar;29(3):657-67 [11301025] Science. 1998 Apr 3;280(5360):69-77 [9525859] EMBO J. 1998 Jun 1;17(11):3016-28 [9606184] J Biol Chem. 1998 Jun 12;273(24):15177-82 [9614131] Eur J Pharmacol. 1998 Apr 17;347(1):141-4 [9650860] Science. 1998 Dec 18;282(5397):2220-6 [9856938] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Needs and opportunities for research in hypersensitivity pneumonitis. AN - 67556038; 15657460 AB - Hypersensitivity pneumonitis (HP) develops after inhalation of many different environmental antigens, causing variable clinical symptoms that often make diagnosis uncertain. The prevalence of HP is higher than recognized, especially its chronic form. Mechanisms of disease are still incompletely known. Strategies to improve detection and diagnosis are needed, and treatment options, principally avoidance, are limited. A workshop recommended: a population-based study to more accurately document the incidence and prevalence of HP; better classification of disease stages, including natural history; evaluation of diagnostic tests and biomarkers used to detect disease; better correlation of computerized tomography lung imaging and pathologic changes; more study of inflammatory and immune mechanisms; and improvement of animal models that are more relevant for human disease. JF - American journal of respiratory and critical care medicine AU - Fink, Jordan N AU - Ortega, Hector G AU - Reynolds, Herbert Y AU - Cormier, Yvon F AU - Fan, Leland L AU - Franks, Teri J AU - Kreiss, Kathleen AU - Kunkel, Steven AU - Lynch, David AU - Quirce, Santiago AU - Rose, Cecile AU - Schleimer, Robert P AU - Schuyler, Mark R AU - Selman, Moises AU - Trout, Douglas AU - Yoshizawa, Yasuyuki AD - DLD/NHLBI, Two Rockledge Center, 6701 Rockledge Drive, Bethesda, MD 20892-7952, USA. Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 792 EP - 798 VL - 171 IS - 7 SN - 1073-449X, 1073-449X KW - Abridged Index Medicus KW - Index Medicus KW - Severity of Illness Index KW - Animals KW - Health Services Needs and Demand KW - Humans KW - Tomography, X-Ray Computed KW - Prognosis KW - Disease Models, Animal KW - Child KW - Mice KW - Child, Preschool KW - Risk Assessment KW - Adult KW - Occupational Exposure -- adverse effects KW - Environmental Exposure -- adverse effects KW - Research -- trends KW - Alveolitis, Extrinsic Allergic -- etiology KW - Alveolitis, Extrinsic Allergic -- epidemiology KW - Alveolitis, Extrinsic Allergic -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67556038?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+respiratory+and+critical+care+medicine&rft.atitle=Needs+and+opportunities+for+research+in+hypersensitivity+pneumonitis.&rft.au=Fink%2C+Jordan+N%3BOrtega%2C+Hector+G%3BReynolds%2C+Herbert+Y%3BCormier%2C+Yvon+F%3BFan%2C+Leland+L%3BFranks%2C+Teri+J%3BKreiss%2C+Kathleen%3BKunkel%2C+Steven%3BLynch%2C+David%3BQuirce%2C+Santiago%3BRose%2C+Cecile%3BSchleimer%2C+Robert+P%3BSchuyler%2C+Mark+R%3BSelman%2C+Moises%3BTrout%2C+Douglas%3BYoshizawa%2C+Yasuyuki&rft.aulast=Fink&rft.aufirst=Jordan&rft.date=2005-04-01&rft.volume=171&rft.issue=7&rft.spage=792&rft.isbn=&rft.btitle=&rft.title=American+journal+of+respiratory+and+critical+care+medicine&rft.issn=1073449X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-08 N1 - Date created - 2005-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Regions of the varicella-zoster virus open reading frame 63 latency-associated protein important for replication in vitro are also critical for efficient establishment of latency. AN - 67554177; 15795292 AB - Varicella-zoster virus (VZV) open reading frame 63 (ORF63) is one of the most abundant transcripts expressed during VZV latency in humans, and ORF63 protein has been detected in human ganglia by several laboratories. Deletion of over 90% of the ORF63 gene showed that the protein is required for efficient establishment of latency in rodents. We have constructed viruses with a series of mutations in ORF63. While prior experiments showed that transfection of cells with a plasmid expressing ORF63 but lacking the putative nuclear localization signal of the protein resulted in increased expression of the protein in the cytoplasm, we found that ORF63 protein remained in the nucleus in cells infected with a VZV ORF63 nuclear localization signal deletion mutant. This mutant was not impaired for growth in cell culture or for latency in rodents. Replacement of five serine or threonine phosphorylation sites in ORF63 with alanines resulted in a virus that was impaired for replication in vitro and for latency. A series of ORF63 carboxy-terminal mutants showed that the last 70 amino acids do not affect replication in vitro or latency in rodents; however, the last 108 amino acids are important for replication and latency. Thus, regions of ORF63 that are important for replication in vitro are also required for efficient establishment of latency. JF - Journal of virology AU - Cohen, Jeffrey I AU - Krogmann, Tammy AU - Bontems, Sebastien AU - Sadzot-Delvaux, Catherine AU - Pesnicak, Lesley AD - Laboratory of Clinical Infectious Diseases, Bldg. 10, Room 11N228, National Institutes of Health, 10 Center Dr., Bethesda, MD 20892, USA. jcohen@niaid.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 5069 EP - 5077 VL - 79 IS - 8 SN - 0022-538X, 0022-538X KW - Immediate-Early Proteins KW - 0 KW - Viral Envelope Proteins KW - immediate early protein 63, Human herpesvirus 3 KW - Index Medicus KW - Animals KW - Cell Nucleus -- virology KW - Humans KW - Transcription, Genetic KW - Cell Line, Tumor KW - Cosmids KW - Sigmodontinae KW - Cloning, Molecular KW - Melanoma KW - Mutagenesis KW - Ganglia -- virology KW - Phosphorylation KW - Restriction Mapping KW - Herpesvirus 3, Human -- growth & development KW - Virus Latency -- genetics KW - Herpesvirus 3, Human -- genetics KW - Virus Replication -- genetics KW - Immediate-Early Proteins -- genetics KW - Open Reading Frames -- genetics KW - Herpesvirus 3, Human -- physiology KW - Viral Envelope Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67554177?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=Regions+of+the+varicella-zoster+virus+open+reading+frame+63+latency-associated+protein+important+for+replication+in+vitro+are+also+critical+for+efficient+establishment+of+latency.&rft.au=Cohen%2C+Jeffrey+I%3BKrogmann%2C+Tammy%3BBontems%2C+Sebastien%3BSadzot-Delvaux%2C+Catherine%3BPesnicak%2C+Lesley&rft.aulast=Cohen&rft.aufirst=Jeffrey&rft.date=2005-04-01&rft.volume=79&rft.issue=8&rft.spage=5069&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-17 N1 - Date created - 2005-03-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):2122-4 [8700895] J Virol. 1996 May;70(5):2789-96 [8627753] Virology. 1998 Jul 5;246(2):306-16 [9657949] Virology. 1999 Jun 5;258(2):451-4 [10366583] J Virol. 2000 Dec;74(24):11464-71 [11090142] J Virol. 2000 Dec;74(24):11893-8 [11090189] J Virol. 2001 Sep;75(17):8224-39 [11483768] J Virol. 2001 Sep;75(18):8854-8 [11507231] Virology. 2001 Oct 25;289(2):218-23 [11689044] J Biol Chem. 2002 Jun 7;277(23):21050-60 [11912195] J Virol. 2002 Nov;76(21):11012-23 [12368344] Virology. 2002 Oct 10;302(1):71-82 [12429517] J Virol. 2003 Jun;77(12):6660-5 [12767985] J Virol. 2003 Oct;77(20):11180-5 [14512565] J Virol. 2004 Feb;78(3):1181-94 [14722273] J Virol. 2004 Nov;78(21):11833-40 [15479825] J Gen Virol. 1986 Sep;67 ( Pt 9):1759-816 [3018124] Virology. 1993 Mar;193(1):193-200 [7679857] Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7376-80 [8394020] J Virol. 1995 Jan;69(1):476-91 [7983744] J Virol. 1995 May;69(5):3240-5 [7707559] J Virol. 1995 Jul;69(7):4274-82 [7769688] J Virol. 1995 Nov;69(11):7367-70 [7474171] J Virol. 1996 Jan;70(1):658-62 [8523589] J Virol. 1996 Feb;70(2):1050-60 [8551563] Neurology. 1995 Dec;45(12 Suppl 8):S18-20 [8545010] Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7080-5 [9618542] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Efficacy and safety of generic amphotericin B in experimental pulmonary aspergillosis. AN - 67553699; 15793161 AB - The recent shortage of the brand name drug Fungizone has necessitated a change to generic formulations of amphotericin B deoxycholate. Clinical trials cannot be conducted in a timely manner to provide data on the safety and efficacy of these formulations. We therefore compared generic amphotericin B and Fungizone for activity and safety in the treatment of experimental invasive pulmonary aspergillosis (IPA) in persistently neutropenic rabbits. Fungizone and generic amphotericin B are similar in efficacy, pharmacokinetics, and safety in the treatment of experimental IPA. JF - Antimicrobial agents and chemotherapy AU - Petraitis, Vidmantas AU - Petraitiene, Ruta AU - Lin, Pengxin AU - Calis, Karim AU - Kelaher, Amy M AU - Muray, Heidi A AU - Mya-San, Christine AU - Mickiene, Diana AU - Bacher, John AU - Walsh, Thomas J AD - Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Building 10, CRC, Rm. 1W-5740, 10 Center Dr., MSC 1100, Bethesda, Maryland 20892-1100, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 1642 EP - 1645 VL - 49 IS - 4 SN - 0066-4804, 0066-4804 KW - Antifungal Agents KW - 0 KW - Drugs, Generic KW - Amphotericin B KW - 7XU7A7DROE KW - Index Medicus KW - Animals KW - Neutropenia -- complications KW - Rabbits KW - Antifungal Agents -- adverse effects KW - Lung Diseases, Fungal -- drug therapy KW - Drugs, Generic -- therapeutic use KW - Drugs, Generic -- adverse effects KW - Aspergillosis -- drug therapy KW - Amphotericin B -- adverse effects KW - Amphotericin B -- therapeutic use KW - Antifungal Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67553699?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+agents+and+chemotherapy&rft.atitle=Efficacy+and+safety+of+generic+amphotericin+B+in+experimental+pulmonary+aspergillosis.&rft.au=Petraitis%2C+Vidmantas%3BPetraitiene%2C+Ruta%3BLin%2C+Pengxin%3BCalis%2C+Karim%3BKelaher%2C+Amy+M%3BMuray%2C+Heidi+A%3BMya-San%2C+Christine%3BMickiene%2C+Diana%3BBacher%2C+John%3BWalsh%2C+Thomas+J&rft.aulast=Petraitis&rft.aufirst=Vidmantas&rft.date=2005-04-01&rft.volume=49&rft.issue=4&rft.spage=1642&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+agents+and+chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-12 N1 - Date created - 2005-03-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Pharmacotherapy. 2003 May;23(5):572-8 [12741430] Lab Anim Sci. 1988 Aug;38(4):467-71 [3184859] Infect Dis Clin North Am. 1989 Sep;3(3):641-51 [2671144] Chemotherapy. 1989;35(5):320-5 [2791709] Clin Infect Dis. 1992 Mar;14 Suppl 1:S43-53 [1562695] Cancer. 1992 Jun 1;69(11):2653-62 [1315207] Clin Microbiol Infect. 2001;7 Suppl 2:25-31 [11525215] J Infect. 1996 Jul;33(1):23-32 [8842991] J Clin Microbiol. 1997 Jan;35(1):139-43 [8968895] J Infect Dis. 1997 Jun;175(6):1459-66 [9180187] Clin Microbiol Rev. 1999 Apr;12(2):310-50 [10194462] J Infect Dis. 2000 Jul;182(1):274-82 [10882607] J Infect Dis. 1994 Feb;169(2):356-68 [8106769] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Aggregated alpha-synuclein activates microglia: a process leading to disease progression in Parkinson's disease. AN - 67553696; 15791003 AB - A growing body of evidence indicates that an inflammatory process in the substantia nigra, characterized by activation of resident microglia, likely either initiates or aggravates nigral neurodegeneration in Parkinson's disease (PD). To study the mechanisms by which nigral microglia are activated in PD, the potential role of alpha-synuclein (a major component of Lewy bodies that can cause neurodegeneration when aggregated) in microglial activation was investigated. The results demonstrated that in a primary mesencephalic neuron-glia culture system, extracellular aggregated human alpha-synuclein indeed activated microglia; microglial activation enhanced dopaminergic neurodegeneration induced by aggregated alpha-synuclein. Furthermore, microglial enhancement of alpha-synuclein-mediated neurotoxicity depended on phagocytosis of alpha-synuclein and activation of NADPH oxidase with production of reactive oxygen species. These results suggest that nigral neuronal damage, regardless of etiology, may release aggregated alpha-synuclein into substantia nigra, which activates microglia with production of proinflammatory mediators, thereby leading to persistent and progressive nigral neurodegeneration in PD. Finally, NADPH oxidase could be an ideal target for potential pharmaceutical intervention, given that it plays a critical role in alpha-synuclein-mediated microglial activation and associated neurotoxicity. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Zhang, Wei AU - Wang, Tongguang AU - Pei, Zhong AU - Miller, David S AU - Wu, Xuefei AU - Block, Michelle L AU - Wilson, Belinda AU - Zhang, Wanqin AU - Zhou, Yong AU - Hong, Jau-Shyong AU - Zhang, Jing AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences/National Institutes of Health, Research Triangle Park, North Carolina, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 533 EP - 542 VL - 19 IS - 6 KW - Nitrites KW - 0 KW - Polymers KW - Reactive Oxygen Species KW - Recombinant Proteins KW - Tumor Necrosis Factor-alpha KW - alpha-Synuclein KW - Tritium KW - 10028-17-8 KW - Nitric Oxide KW - 31C4KY9ESH KW - gamma-Aminobutyric Acid KW - 56-12-2 KW - Tyrosine 3-Monooxygenase KW - EC 1.14.16.2 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Dinoprostone KW - K7Q1JQR04M KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Nitrites -- analysis KW - NADPH Oxidase -- deficiency KW - Polymers -- pharmacology KW - Humans KW - Dopamine -- physiology KW - Nerve Degeneration -- chemically induced KW - Nitric Oxide -- metabolism KW - Neuroglia -- physiology KW - Mice, Knockout KW - Rats KW - Tyrosine 3-Monooxygenase -- analysis KW - Mesencephalon KW - Rats, Inbred F344 KW - Oxidative Stress KW - Neurons -- physiology KW - NADPH Oxidase -- physiology KW - gamma-Aminobutyric Acid -- metabolism KW - Phagocytosis KW - Embryo, Mammalian KW - Enzyme Activation KW - Brain KW - Astrocytes -- physiology KW - Mice KW - Cells, Cultured KW - Dinoprostone -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Immunohistochemistry KW - Substantia Nigra -- metabolism KW - Parkinson Disease -- etiology KW - alpha-Synuclein -- pharmacology KW - Microglia -- physiology KW - alpha-Synuclein -- chemistry KW - alpha-Synuclein -- physiology KW - Microglia -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67553696?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=Aggregated+alpha-synuclein+activates+microglia%3A+a+process+leading+to+disease+progression+in+Parkinson%27s+disease.&rft.au=Zhang%2C+Wei%3BWang%2C+Tongguang%3BPei%2C+Zhong%3BMiller%2C+David+S%3BWu%2C+Xuefei%3BBlock%2C+Michelle+L%3BWilson%2C+Belinda%3BZhang%2C+Wanqin%3BZhou%2C+Yong%3BHong%2C+Jau-Shyong%3BZhang%2C+Jing&rft.aulast=Zhang&rft.aufirst=Wei&rft.date=2005-04-01&rft.volume=19&rft.issue=6&rft.spage=533&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-14 N1 - Date created - 2005-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microglial NADPH oxidase is a novel target for femtomolar neuroprotection against oxidative stress. AN - 67553024; 15791005 AB - Inflammation has been increasingly recognized to contribute to the pathogenesis of Parkinson's disease. Several compounds are neuroprotective at femtomolar concentrations through the inhibition of inflammation. However, the mechanisms mediating femtomolar-acting compounds are poorly understood. Here we show that both gly-gly-phe (GGF), a tri-peptide contained in the dynorphin opioid peptide, and naloxone are neuroprotective at femtomolar concentrations against LPS-induced dopaminergic neurotoxicity through the reduction of microglial activation. Mechanistic studies demonstrated the critical role of NADPH oxidase in the GGF and naloxone inhibition of microglial activation and associated DA neurotoxicity. Pharmacophore analysis of the neuroprotective dynorphin peptides and naloxone revealed common chemical properties (hydrogen bond acceptor, hydrogen bond donor, positive ionizable, hydrophobic) of these femtomolar-acting compounds. These results support a common high-affinity site of action for several femtomolar-acting compounds, where NADPH oxidase is the critical mechanism governing neuroprotection, suggesting a novel avenue of anti-inflammatory and neuroprotective therapy. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Qin, Liya AU - Block, Michelle L AU - Liu, Yuxin AU - Bienstock, Rachelle J AU - Pei, Zhong AU - Zhang, Wei AU - Wu, Xuefei AU - Wilson, Belinda AU - Burka, Tom AU - Hong, Jau-Shyong AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 550 EP - 557 VL - 19 IS - 6 KW - Enzyme Inhibitors KW - 0 KW - Lipopolysaccharides KW - Neuroprotective Agents KW - Peptide Fragments KW - Reactive Oxygen Species KW - Tumor Necrosis Factor-alpha KW - Superoxides KW - 11062-77-4 KW - Naloxone KW - 36B82AMQ7N KW - Dynorphins KW - 74913-18-1 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Naloxone -- pharmacology KW - Naloxone -- administration & dosage KW - Animals KW - Hydrophobic and Hydrophilic Interactions KW - Naloxone -- chemistry KW - Lipopolysaccharides -- pharmacology KW - Amino Acid Sequence KW - Mice KW - Structure-Activity Relationship KW - Mice, Knockout KW - Rats KW - Superoxides -- metabolism KW - Rats, Inbred F344 KW - Peptide Fragments -- chemistry KW - Dynorphins -- administration & dosage KW - Peptide Fragments -- pharmacology KW - Molecular Sequence Data KW - Dynorphins -- chemistry KW - Mice, Inbred C57BL KW - Enzyme Inhibitors -- pharmacology KW - Tumor Necrosis Factor-alpha -- metabolism KW - Hydrogen Bonding KW - Dynorphins -- pharmacology KW - Microglia -- enzymology KW - NADPH Oxidase -- deficiency KW - NADPH Oxidase -- antagonists & inhibitors KW - Oxidative Stress -- drug effects KW - NADPH Oxidase -- physiology KW - Microglia -- drug effects KW - Neuroprotective Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67553024?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=Microglial+NADPH+oxidase+is+a+novel+target+for+femtomolar+neuroprotection+against+oxidative+stress.&rft.au=Qin%2C+Liya%3BBlock%2C+Michelle+L%3BLiu%2C+Yuxin%3BBienstock%2C+Rachelle+J%3BPei%2C+Zhong%3BZhang%2C+Wei%3BWu%2C+Xuefei%3BWilson%2C+Belinda%3BBurka%2C+Tom%3BHong%2C+Jau-Shyong&rft.aulast=Qin&rft.aufirst=Liya&rft.date=2005-04-01&rft.volume=19&rft.issue=6&rft.spage=550&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-14 N1 - Date created - 2005-03-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tumor necrosis factor enhances SN38-mediated apoptosis in mesothelioma cells: the role of nuclear factor-kappaB pathway activation. AN - 67547722; 15700263 AB - Despite the best and most aggressive, often integrated, standard therapeutic approaches for mesothelioma, overall survival remains very poor. The actual failure points out clearly the need for the development of novel therapy. One of the promising paths of experimentation is artificial induction of apoptosis. A therapeutic strategy that relies on the down-regulation of BCL-XL inhibition nuclear factor kappaB (NF-kappaB) with a combination of SN38 and tumor necrosis factor (TNF) was studied in human mesothelioma cell lines (MSTO-221H, IST-MES1, IST-MES2, MPP89, H28, H513, H2052, and H290). Cell proliferation (clonogenic assay) was inhibited strongly by the combination of TNF and SN38. Examining the persistence of the NF-kappaB complexes using an electrophoretic mobility-shift assay, it appeared that they still were present at 24 hours in TNF-treated cells. In SN38-treated cells, NF-kappaB complexes persisted for 6 hours. In cells that were treated with combined SN38 and TNF, NF-kappaB complexes disappeared quickly and became undetectable at 6 hours. In flow cytometry analysis, only cells that were treated with combined SN38 and TNF demonstrated significant cellular accumulation in the sub-G0-G1 phase, suggesting a specific induction of apoptosis. Morphologic examination (4,6-diamidino-2-phenylindole staining and electron microscopy) and internucleosomal DNA fragmentation (gel ladder) confirmed rigorously the induction of apoptosis. Because of NF-kappaB inhibition with the combination of SN38 and TNF, the expression of BCL-XL (both the protein [Western blot analysis] and the mRNA [reverse transcriptase-polymerase chain reaction analysis]) was down-regulated, cytochrome c was released into the cytoplasm, caspase 3 was activated (Western blot analysis), and, consequently, apoptosis was triggered. The authors hope that the results of the current study may contribute to the design and implementation of a novel therapeutic approach that improves patients' responses to treatment for mesothelioma. Copyright 2005 American Cancer Society. JF - Cancer AU - Russo, Patrizia AU - Catassi, Alessia AU - Malacarne, Davide AU - Margaritora, Stefano AU - Cesario, Alfredo AU - Festi, Luigi AU - Mulé, Antonino AU - Ferri, Luigi AU - Granone, Pierluigi AD - Laboratory of Translational Research B(Lung Cancer), Department of Integrated Medical Oncology, National Cancer Institute, Genoa, Italy. patrizia.russo@istge.it Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 1503 EP - 1518 VL - 103 IS - 7 SN - 0008-543X, 0008-543X KW - BCL2L1 protein, human KW - 0 KW - NF-kappa B KW - Proto-Oncogene Proteins c-bcl-2 KW - Tumor Necrosis Factors KW - bcl-X Protein KW - irinotecan KW - 0H43101T0J KW - Cytochromes c KW - 9007-43-6 KW - Caspases KW - EC 3.4.22.- KW - Camptothecin KW - XT3Z54Z28A KW - Abridged Index Medicus KW - Index Medicus KW - Cytochromes c -- metabolism KW - Tumor Cells, Cultured KW - Cell Survival -- drug effects KW - DNA Damage KW - Humans KW - Proto-Oncogene Proteins c-bcl-2 -- metabolism KW - Drug Synergism KW - DNA Fragmentation KW - Cell Cycle -- drug effects KW - Caspases -- metabolism KW - Mesothelioma -- drug therapy KW - Camptothecin -- pharmacology KW - Apoptosis -- drug effects KW - Camptothecin -- analogs & derivatives KW - Tumor Necrosis Factors -- pharmacology KW - NF-kappa B -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67547722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer&rft.atitle=Tumor+necrosis+factor+enhances+SN38-mediated+apoptosis+in+mesothelioma+cells%3A+the+role+of+nuclear+factor-kappaB+pathway+activation.&rft.au=Russo%2C+Patrizia%3BCatassi%2C+Alessia%3BMalacarne%2C+Davide%3BMargaritora%2C+Stefano%3BCesario%2C+Alfredo%3BFesti%2C+Luigi%3BMul%C3%A9%2C+Antonino%3BFerri%2C+Luigi%3BGranone%2C+Pierluigi&rft.aulast=Russo&rft.aufirst=Patrizia&rft.date=2005-04-01&rft.volume=103&rft.issue=7&rft.spage=1503&rft.isbn=&rft.btitle=&rft.title=Cancer&rft.issn=0008543X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-05 N1 - Date created - 2005-03-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Retraction In: Cancer. 2010 May 1;116(9):2285 [20229574] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Steroid hormone receptor expression and proliferation in rat mammary gland carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. AN - 67536030; 15637090 AB - 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mammary gland carcinogen present in the human diet. Herein, the expression of estrogen receptor alpha (ERalpha), estrogen receptor beta (ER beta) and progesterone receptor (PR) was examined in mammary gland carcinomas induced by PhIP in female Sprague-Dawley rats. Quantitative real-time polymerase chain reaction demonstrated that ER alpha, ER beta and PR were statistically elevated by 3-, 4- and 8-fold in carcinomas compared with normal mammary glands. By immunohistochemistry, carcinomas showed statistically higher nuclear expression of all three steroid receptors with the majority of carcinomas showing at least 10% of epithelial cells stained for ER alpha (49/55, 89%), ER beta (41/55, 75%) and PR (48/55, 87%). Furthermore, the level of expression of the three steroid hormone receptors was positively correlated with each other across the bank of carcinomas (Spearman analysis, P < 0.05). The expression of ER alpha in carcinomas was associated with tumor grade, extent of nuclear pleomorphism and cellular proliferation as measured by proliferating cell nuclear antigen (PCNA) and phospho-Rb immunostaining (Spearman analysis, P < 0.05). Confocal microscopy was used to measure the percentage of epithelial cells showing nuclear colocalization of receptors, PCNA, and cyclin D1. Colocalization of the receptors, and the colocalization of the receptors with PCNA and cyclin D1 was strikingly higher in carcinomas than in the normal mammary gland. In carcinoma cells, 37% of ER alpha positive epithelial cells were colocalized with PCNA in contrast to just 0.25% of cells in the normal mammary gland. The findings from this study indicate that ER alpha, ER beta and PR were co-upregulated and nuclear localized in epithelial cells from rat mammary carcinomas compared with normal mammary glands, and that the co-upregulation was positively correlated with proliferation and cell cycle progression in carcinomas. JF - Carcinogenesis AU - Qiu, Cunping AU - Shan, Liang AU - Yu, Minshu AU - Snyderwine, Elizabeth G AD - Chemical Carcinogenesis Section, Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 763 EP - 769 VL - 26 IS - 4 SN - 0143-3334, 0143-3334 KW - Carcinogens KW - 0 KW - Estrogen Receptor alpha KW - Estrogen Receptor beta KW - Imidazoles KW - Proliferating Cell Nuclear Antigen KW - RNA, Messenger KW - Receptors, Progesterone KW - Retinoblastoma Protein KW - Cyclin D1 KW - 136601-57-5 KW - 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine KW - 909C6UN66T KW - Index Medicus KW - Animals KW - Cell Nucleus -- metabolism KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Messenger -- genetics KW - Rats KW - Epithelial Cells -- ultrastructure KW - Rats, Sprague-Dawley KW - Cyclin D1 -- metabolism KW - RNA, Messenger -- metabolism KW - Mammary Glands, Animal -- metabolism KW - Mammary Glands, Animal -- pathology KW - Retinoblastoma Protein -- metabolism KW - Cell Cycle -- drug effects KW - Female KW - Immunoenzyme Techniques KW - Proliferating Cell Nuclear Antigen -- metabolism KW - Receptors, Progesterone -- genetics KW - Cell Proliferation -- drug effects KW - Imidazoles -- toxicity KW - Receptors, Progesterone -- metabolism KW - Estrogen Receptor alpha -- genetics KW - Carcinogens -- toxicity KW - Estrogen Receptor alpha -- metabolism KW - Estrogen Receptor beta -- metabolism KW - Mammary Neoplasms, Experimental -- metabolism KW - Mammary Neoplasms, Experimental -- pathology KW - Estrogen Receptor beta -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67536030?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Steroid+hormone+receptor+expression+and+proliferation+in+rat+mammary+gland+carcinomas+induced+by+2-amino-1-methyl-6-phenylimidazo%5B4%2C5-b%5Dpyridine.&rft.au=Qiu%2C+Cunping%3BShan%2C+Liang%3BYu%2C+Minshu%3BSnyderwine%2C+Elizabeth+G&rft.aulast=Qiu&rft.aufirst=Cunping&rft.date=2005-04-01&rft.volume=26&rft.issue=4&rft.spage=763&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-03-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thrombopoietin alone or in the presence of stem cell factor supports the growth of KIT(CD117)low/ MPL(CD110)+ human mast cells from hematopoietic progenitor cells. AN - 67534240; 15781331 AB - Thrombopoietin (TPO) is known to promote platelet number, have growth-promoting potential for human megakaryocytes (HuMKs), and increase erythrocyte, monocyte, mast cell, and granulocyte numbers in the presence of additional growth factors. We explored the ability of TPO alone or in the presence of stem cell factor (SCF) to support human mast cells (HuMCs). CD34+ pluripotent and CD34+/CD117+/CD13+ HuMC progenitor cells were cultured in rhTPO and examined for HuMCs. Similarly, we added rhTPO to CD34(+) cells cultured in stem cell factor (SCF), which promotes HuMC development. When CD34+ cells were cultured in 10 ng/mL rhTPO and 10 ng/mL rhSCF, TPO enhanced HuMC numbers compared to rhSCF alone. Higher concentrations of rhTPO (50 ng/mL) in the presence of 100 ng/mL rhSCF inhibited the rhSCF-dependent subpopulation of CD117high HuMCs, while promoting CD117low HuMCs. Human CD34+/CD117+/CD13+ cells cultured in rhTPO alone for 1 to 2 weeks differentiated into CD41+/CD110+ HuMKs (85-90%) and FcepsilonRI+/CD117low/CD13+ HuMCs (5-10%). RhTPO-induced HuMCs expressed the TPO (CD110) receptor, tryptase, and chymase and survived when recultured in rhSCF. The effect of TPO on HuMCs in the presence of rhSCF varies, depending on the relative concentration of each growth factor, while TPO alone or in combination with rhSCF supports a unique population of CD117low/CD110+ HuMCs. JF - Experimental hematology AU - Kirshenbaum, Arnold S AU - Akin, Cem AU - Goff, Julie P AU - Metcalfe, Dean D AD - Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1881, USA. Akirshenba@niaid.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 413 EP - 421 VL - 33 IS - 4 SN - 0301-472X, 0301-472X KW - Proto-Oncogene Proteins KW - 0 KW - Receptors, Cytokine KW - Receptors, Thrombopoietin KW - Stem Cell Factor KW - MPL protein, human KW - 143641-95-6 KW - Thrombopoietin KW - 9014-42-0 KW - Proto-Oncogene Proteins c-kit KW - EC 2.7.10.1 KW - Index Medicus KW - Drug Interactions KW - Cells, Cultured KW - Humans KW - Cell Culture Techniques KW - Flow Cytometry KW - Immunophenotyping KW - Cell Proliferation -- drug effects KW - Thrombopoietin -- pharmacology KW - Stem Cell Factor -- pharmacology KW - Hematopoietic Stem Cells -- cytology KW - Mast Cells -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67534240?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+hematology&rft.atitle=Thrombopoietin+alone+or+in+the+presence+of+stem+cell+factor+supports+the+growth+of+KIT%28CD117%29low%2F+MPL%28CD110%29%2B+human+mast+cells+from+hematopoietic+progenitor+cells.&rft.au=Kirshenbaum%2C+Arnold+S%3BAkin%2C+Cem%3BGoff%2C+Julie+P%3BMetcalfe%2C+Dean+D&rft.aulast=Kirshenbaum&rft.aufirst=Arnold&rft.date=2005-04-01&rft.volume=33&rft.issue=4&rft.spage=413&rft.isbn=&rft.btitle=&rft.title=Experimental+hematology&rft.issn=0301472X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-18 N1 - Date created - 2005-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mortality among participants in the agricultural health study. AN - 67534049; 15780775 AB - This analysis of the Agricultural Health Study cohort assesses the mortality experience of licensed pesticide applicators and their spouses. This report is based on 52,393 private applicators (who are mostly farmers) and 32,345 spouses of farmers in Iowa and North Carolina. At enrollment, each pesticide applicator completed a 21-page enrollment questionnaire. Mortality assessment from enrollment (1994-1997) through 2000 provided an average follow-up of about 5.3 years, 447,154 person-years, and 2055 deaths. Compared with the general population in the two states, the cohort experienced a very low mortality rate. Standardized mortality ratios (SMRs) for total mortality, cardiovascular disease, diabetes, COPD, total cancer, and cancers of the esophagus, stomach, and lung were 0.6 or lower for both farmers and spouses. These deficits varied little by farm size, type of crops or livestock on the farm, years of handling pesticides, holding a non-farm job, or length of follow up. SMRs among ever smokers were not as low as among never smokers, but were still less than 1.0 for all smoking-related causes of death. No statistically significant excesses occurred, but slightly elevated SMRs, or those near 1.0, were noted for diseases that have been associated with farming in previous studies. Several factors may contribute to the low mortality observed in this population, including the healthy worker effect typically seen in cohorts of working populations (which may decline in future years), a short follow-up interval, and a healthier lifestyle manifested through lower cigarette use and an occupation that has traditionally required high levels of physical activity. JF - Annals of epidemiology AU - Blair, Aaron AU - Sandler, Dale P AU - Tarone, Robert AU - Lubin, Jay AU - Thomas, Kent AU - Hoppin, Jane A AU - Samanic, Claudine AU - Coble, Joseph AU - Kamel, Freya AU - Knott, Charles AU - Dosemeci, Mustafa AU - Zahm, Shelia Hoar AU - Lynch, Charles F AU - Rothman, Nathaniel AU - Alavanja, Michael C R AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, Bethesda, MD, USA. blaira@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 279 EP - 285 VL - 15 IS - 4 SN - 1047-2797, 1047-2797 KW - Pesticides KW - 0 KW - Index Medicus KW - Occupational Exposure KW - Humans KW - Cohort Studies KW - Middle Aged KW - North Carolina -- epidemiology KW - Male KW - Iowa -- epidemiology KW - Female KW - Cause of Death KW - Spouses KW - Agriculture KW - Disease -- classification KW - Neoplasms -- classification KW - Neoplasms -- mortality KW - Pesticides -- poisoning KW - Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67534049?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+epidemiology&rft.atitle=Mortality+among+participants+in+the+agricultural+health+study.&rft.au=Blair%2C+Aaron%3BSandler%2C+Dale+P%3BTarone%2C+Robert%3BLubin%2C+Jay%3BThomas%2C+Kent%3BHoppin%2C+Jane+A%3BSamanic%2C+Claudine%3BCoble%2C+Joseph%3BKamel%2C+Freya%3BKnott%2C+Charles%3BDosemeci%2C+Mustafa%3BZahm%2C+Shelia+Hoar%3BLynch%2C+Charles+F%3BRothman%2C+Nathaniel%3BAlavanja%2C+Michael+C+R&rft.aulast=Blair&rft.aufirst=Aaron&rft.date=2005-04-01&rft.volume=15&rft.issue=4&rft.spage=279&rft.isbn=&rft.btitle=&rft.title=Annals+of+epidemiology&rft.issn=10472797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Molecular determinants of the agonist binding domain of a P2X receptor channel. AN - 67533877; 15632318 AB - P2 purinergic receptor channel receptors (P2XRs) are a family of ligand-gated cation channels composed of two transmembrane domains, N and C termini located intracellularly, and a large extracellular loop containing the ATP binding domain. To identify regions important for binding and gating, previous experimental work was focused on mutagenesis of conserved ectodomain residues. Here, we used the known sequence and secondary structure similarities between the Lys180-Lys326 ectodomain region of P2X(4) and the class II aminoacyl-tRNA synthetases as a guide to generate a three-dimensional model of the receptor-binding site and to design mutants. The interplay between homology modeling and site-directed mutagenesis suggested that Asp280 residue of P2X(4)R coordinates ATP binding via the magnesium ion, Phe230 residue coordinates the binding of the adenine ring of ATP, and Lys190, His286, and Arg278 residues coordinate the actions of negatively charged alpha-, beta-, and gamma-phosphate groups, respectively. Until the crystal structure of the channel is solved, this model could provide a useful approach for future studies on the identification of ATP binding domain and gating of P2XRs. JF - Molecular pharmacology AU - Yan, Zonghe AU - Liang, Zhaodong AU - Tomic, Melanija AU - Obsil, Tomas AU - Stojilkovic, Stanko S AD - Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development/NIH, Building 49, Room 6A-36, 49 Convent Drive, Bethesda, MD 20892-4510, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 1078 EP - 1088 VL - 67 IS - 4 SN - 0026-895X, 0026-895X KW - Purinergic P2 Receptor Agonists KW - 0 KW - Receptors, Purinergic P2 KW - Receptors, Purinergic P2X KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Index Medicus KW - Rats KW - Mutagenesis, Site-Directed KW - Animals KW - Models, Molecular KW - Guinea Pigs KW - Cells, Cultured KW - Humans KW - Mice KW - Binding Sites KW - Receptors, Purinergic P2 -- metabolism KW - Receptors, Purinergic P2 -- chemistry KW - Adenosine Triphosphate -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67533877?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+pharmacology&rft.atitle=Molecular+determinants+of+the+agonist+binding+domain+of+a+P2X+receptor+channel.&rft.au=Yan%2C+Zonghe%3BLiang%2C+Zhaodong%3BTomic%2C+Melanija%3BObsil%2C+Tomas%3BStojilkovic%2C+Stanko+S&rft.aulast=Yan&rft.aufirst=Zonghe&rft.date=2005-04-01&rft.volume=67&rft.issue=4&rft.spage=1078&rft.isbn=&rft.btitle=&rft.title=Molecular+pharmacology&rft.issn=0026895X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-20 N1 - Date created - 2005-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Insurance coverage, usual source of care, and receipt of clinically indicated care for comorbid conditions among adults living with human immunodeficiency virus. AN - 67532702; 15778643 AB - Associations of insurance coverage and source of care with use of human immunodeficiency virus (HIV)-related health, mental health, and substance abuse services are examined in a large, diverse, highly active antiretroviral therapy-era cohort. Adults who were infected with HIV (n = 3818) were interviewed in clinics and community agencies in Los Angeles, Milwaukee, New York, and San Francisco regarding drug use behaviors, health status, and health care utilization. Most participants were insured by Medicaid. During the previous 3 months, 90% of privately insured, 87% of publicly insured, and 78% of uninsured participants had visited any provider. Publicly and privately insured participants were similar in receipt of antiretrovirals, prophylaxis against Pneumocystis carinii pneumonia, substance abuse services, and antidepressants. Uninsured participants were less likely to receive antiretrovirals but were more likely to use substance abuse services. Participants with no usual source of care were less likely to receive PCP prophylaxis. A lack of insurance is associated with barriers to care, but the advantage of private over public coverage appears smaller than in previous studies. PCP prophylaxis, substance abuse treatment, and antidepressants remain markedly underutilized. Educational initiatives about these treatments targeting providers and patients are indicated. JF - Medical care AU - Goldstein, Risë B AU - Rotheram-Borus, Mary Jane AU - Johnson, Mallory O AU - Weinhardt, Lance S AU - Remien, Robert H AU - Lightfoot, Marguerita AU - Catz, Sheryl L AU - Gore-Felton, Cheryl AU - Kirshenbaum, Sheri AU - Morin, Stephen F AU - NIMH Healthy Living Trial Group AD - Center for Community Health, UCLA Neuropsychiatric Institute, Los Angeles, California, USA. goldster@mail.nih.gov ; NIMH Healthy Living Trial Group Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 401 EP - 410 VL - 43 IS - 4 SN - 0025-7079, 0025-7079 KW - Anti-Retroviral Agents KW - 0 KW - Antidepressive Agents KW - Index Medicus KW - Private Practice -- utilization KW - Health Maintenance Organizations -- utilization KW - Pneumonia, Pneumocystis -- economics KW - Antibiotic Prophylaxis -- utilization KW - Depression -- economics KW - Humans KW - AIDS-Related Opportunistic Infections -- prevention & control KW - Substance-Related Disorders -- economics KW - Anti-Retroviral Agents -- therapeutic use KW - Substance-Related Disorders -- prevention & control KW - Depression -- prevention & control KW - Pneumonia, Pneumocystis -- prevention & control KW - Antiretroviral Therapy, Highly Active -- utilization KW - Adult KW - Depression -- epidemiology KW - Antidepressive Agents -- therapeutic use KW - United States -- epidemiology KW - AIDS-Related Opportunistic Infections -- economics KW - Male KW - Ambulatory Care Facilities -- utilization KW - Female KW - Substance-Related Disorders -- epidemiology KW - HIV Infections -- complications KW - Health Services Accessibility -- economics KW - HIV Infections -- therapy KW - Insurance, Health KW - Health Services -- utilization KW - Medicaid KW - HIV Infections -- economics KW - Insurance Coverage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67532702?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diagnostic+Microbiology+and+Infectious+Disease&rft.atitle=Use+of+housekeeping+gene+sequencing+for+species+identification+of+viridans+streptococci&rft.au=Kiratisin%2C+P%3BLi%2C+L%3BMurray%2C+PR%3BFischer%2C+SH&rft.aulast=Kiratisin&rft.aufirst=P&rft.date=2005-04-01&rft.volume=51&rft.issue=4&rft.spage=297&rft.isbn=&rft.btitle=&rft.title=Diagnostic+Microbiology+and+Infectious+Disease&rft.issn=07328893&rft_id=info:doi/10.1016%2Fj.diagmicrobio.2004.12.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-03-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of CD4(+)25+ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide. AN - 67529995; 15591121 AB - Regulatory T cells (T(REGs)) control the key aspects of tolerance and play a role in the lack of antitumor immune responses. Cyclophosphamide (CY) is a chemotherapeutic agent with a dose-dependent, bimodal effect on the immune system. Although a previous study demonstrated that CY reduces the number of T(REGs), the mechanism involved in this process has yet to be defined. In this report, it is established that low-dose CY not only decreases cell number but leads to decreased functionality of T(REGs). CY treatment enhances apoptosis and decreases homeostatic proliferation of these cells. Expression of GITR and FoxP3, which are involved in the suppressive activity of T(REGs), is down-regulated after CY administration, though the level of expression varies depending on the time studied. This is the first report demonstrating that CY, in addition to decreasing cell number, inhibits the suppressive capability of T(REGs). The relevance of the loss of suppressor functionality and the changes in gene expression are further discussed. JF - Blood AU - Lutsiak, M E Christine AU - Semnani, Roshanak T AU - De Pascalis, Roberto AU - Kashmiri, Syed V S AU - Schlom, Jeffrey AU - Sabzevari, Helen AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 10, Rm 8B09, Bethesda, MD 20892, USA. Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 2862 EP - 2868 VL - 105 IS - 7 SN - 0006-4971, 0006-4971 KW - Antigens, Surface KW - 0 KW - DNA-Binding Proteins KW - Forkhead Transcription Factors KW - Foxp3 protein, mouse KW - Glucocorticoid-Induced TNFR-Related Protein KW - Immunosuppressive Agents KW - Receptors, Interleukin-2 KW - Receptors, Nerve Growth Factor KW - Receptors, Tumor Necrosis Factor KW - Tnfrsf18 protein, mouse KW - Cyclophosphamide KW - 8N3DW7272P KW - Abridged Index Medicus KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Receptors, Nerve Growth Factor -- genetics KW - B-Lymphocytes -- cytology KW - Apoptosis -- immunology KW - DNA-Binding Proteins -- genetics KW - Mice KW - B-Lymphocytes -- immunology KW - Antigens, Surface -- genetics KW - Antigens, Surface -- immunology KW - Cell Differentiation -- immunology KW - B-Lymphocytes -- drug effects KW - Receptors, Interleukin-2 -- metabolism KW - Gene Expression -- immunology KW - Apoptosis -- drug effects KW - Mice, Inbred C57BL KW - Cell Differentiation -- drug effects KW - Receptors, Tumor Necrosis Factor -- genetics KW - Female KW - CD4-Positive T-Lymphocytes -- cytology KW - CD4-Positive T-Lymphocytes -- immunology KW - CD4-Positive T-Lymphocytes -- drug effects KW - Immunosuppressive Agents -- pharmacology KW - Cyclophosphamide -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67529995?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Inhibition+of+CD4%28%2B%2925%2B+T+regulatory+cell+function+implicated+in+enhanced+immune+response+by+low-dose+cyclophosphamide.&rft.au=Lutsiak%2C+M+E+Christine%3BSemnani%2C+Roshanak+T%3BDe+Pascalis%2C+Roberto%3BKashmiri%2C+Syed+V+S%3BSchlom%2C+Jeffrey%3BSabzevari%2C+Helen&rft.aulast=Lutsiak&rft.aufirst=M+E&rft.date=2005-04-01&rft.volume=105&rft.issue=7&rft.spage=2862&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dopamine D3 receptor ligands block nicotine-induced conditioned place preferences through a mechanism that does not involve discriminative-stimulus or antidepressant-like effects. AN - 67523833; 15562293 AB - Environmental stimuli previously paired with drug taking appear to play a critical role in nicotine dependence. Converging anatomical, pharmacological, and behavioral evidence implicates dopamine D3 receptors (D3Rs) in the mechanisms underlying stimulus-controlled drug-seeking behavior. This study assessed the effects of BP 897, a D3R partial agonist and ST 198, a D3R antagonist, on nicotine-induced conditioned place preferences (CPPs), used as a measure of drug-seeking behavior, on food-maintained responding and on discrimination performance under a two-lever-choice nicotine discrimination procedure. BP 897 and ST 198 both blocked the expression of nicotine-induced CPP at doses selective for D3R. They had no effect on locomotor activity in the CPP apparatus and no significant effect on nicotine discrimination performance or food-maintained responding under the discrimination procedure. Involvement of antidepressant actions in the effects of BP 897 and ST 198 on CPP is unlikely, since we found no effect of D3R blockade with BP 897 or genetic depletion of D3Rs in a forced swimming test, used as a behavioral test for antidepressant activity. This suggests that D3R ligands reduce the motivational effects of nicotine by a mechanism distinct from those of nicotine replacement therapy and bupropion, the two currently used aids for smoking cessation in humans. These findings support the use of D3R ligands as aids for smoking cessation and indicate that their effects would be selective for those rewarding or reinforcing effects of nicotine that contribute to the maintenance of tobacco-smoking behavior, without affecting subjective responses to nicotine or producing any antidepressant-like effects. JF - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology AU - Le Foll, Bernard AU - Sokoloff, Pierre AU - Stark, Holger AU - Goldberg, Steven R AD - Preclinical Pharmacology Section, Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, MD 21224, USA. blefoll@intra.nida.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 720 EP - 730 VL - 30 IS - 4 SN - 0893-133X, 0893-133X KW - ((E)-N-4-(1,2,3,4-tetrahydroisoquinolin-2-yl)butyl)-3-phenylacrylamide KW - 0 KW - Acrylamides KW - Antidepressive Agents KW - BP 897 KW - Dopamine Agents KW - Dopamine Agonists KW - Dopamine Antagonists KW - Drd3 protein, mouse KW - Drd3 protein, rat KW - Isoquinolines KW - Ligands KW - Piperazines KW - Receptors, Dopamine D2 KW - Receptors, Dopamine D3 KW - Nicotine KW - 6M3C89ZY6R KW - Index Medicus KW - Limbic System -- physiopathology KW - Animals KW - Discrimination (Psychology) -- drug effects KW - Antidepressive Agents -- pharmacology KW - Limbic System -- metabolism KW - Discrimination (Psychology) -- physiology KW - Dopamine Antagonists -- pharmacology KW - Disease Models, Animal KW - Mice KW - Piperazines -- pharmacology KW - Drug Interactions -- physiology KW - Mice, Knockout KW - Conditioning (Psychology) -- drug effects KW - Rats KW - Isoquinolines -- pharmacology KW - Rats, Sprague-Dawley KW - Limbic System -- drug effects KW - Dopamine Agonists -- pharmacology KW - Acrylamides -- pharmacology KW - Conditioning (Psychology) -- physiology KW - Male KW - Female KW - Tobacco Use Disorder -- physiopathology KW - Tobacco Use Disorder -- metabolism KW - Nicotine -- antagonists & inhibitors KW - Spatial Behavior -- drug effects KW - Tobacco Use Disorder -- drug therapy KW - Nicotine -- pharmacology KW - Receptors, Dopamine D2 -- agonists KW - Dopamine Agents -- pharmacology KW - Receptors, Dopamine D2 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67523833?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.atitle=Dopamine+D3+receptor+ligands+block+nicotine-induced+conditioned+place+preferences+through+a+mechanism+that+does+not+involve+discriminative-stimulus+or+antidepressant-like+effects.&rft.au=Le+Foll%2C+Bernard%3BSokoloff%2C+Pierre%3BStark%2C+Holger%3BGoldberg%2C+Steven+R&rft.aulast=Le+Foll&rft.aufirst=Bernard&rft.date=2005-04-01&rft.volume=30&rft.issue=4&rft.spage=720&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-03-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Molecular profiles of mRNA levels in laser capture microdissected murine hippocampal regions differentially responsive to TMT-induced cell death. AN - 67523816; 15773920 AB - Using a chemical-induced model of dentate granule (DG) cell death, cDNA microarray analysis was used to identify gene profiles from the laser-captured microdissected (LCM) hippocampal DG cell region versus the CA pyramidal cell layer (CA) from 21-day-old male CD1 mice injected with trimethyltin hydroxide (TMT; 3.0 mg/kg, i.p.). At 6 h post-TMT, lectin + microglia displaying a reactive morphology were in contact with active caspase 3+ neurons. By 18 h, amoeboid microglia and signs of phagocytosis, and a mild astrocytic response were present in the DG. There was no evidence of IgG extravasation in the hippocampus, or cell death and glial reactivity in the CA. Atlas 1.2K Clontech array detected 115 genes changed in the hippocampus with TMT and included genes associated with immediate-early responses, calcium homeostasis, cellular signaling, cell cycle, immunomodulation and DNA repair. Early responses localized to LCM DG samples consisted of elevations in inflammatory factors such as tumor necrosis factor-alpha and receptors, as well as MIP1alpha, CD14, CD18, and a decrease in factors associated with calcium buffering. By 18 h, in the DG, changes occurred in transcripts associated with apoptosis, cell adhesion, DNA repair, cell proliferation and growth. In the CA, a differential level of elevation was seen in CD86 antigen, zinc finger protein 38 and DNA damage inducible transcript 3. A significant number of genes was decreased at these early time points in both hippocampal regions. JF - Journal of neurochemistry AU - Lefebvre d'Hellencourt, Christian AU - Harry, G Jean AD - Neurotoxicology Group, Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Health and Human Services, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 206 EP - 220 VL - 93 IS - 1 SN - 0022-3042, 0022-3042 KW - Glial Fibrillary Acidic Protein KW - 0 KW - Lectins KW - RNA, Messenger KW - Trimethyltin Compounds KW - trimethyltin hydroxide KW - 56-24-6 KW - Casp3 protein, mouse KW - EC 3.4.22.- KW - Caspase 3 KW - Caspases KW - Phosphopyruvate Hydratase KW - EC 4.2.1.11 KW - Index Medicus KW - Animals KW - Microdissection -- methods KW - Glial Fibrillary Acidic Protein -- metabolism KW - Disease Models, Animal KW - Mice KW - Immunohistochemistry -- methods KW - Reverse Transcriptase Polymerase Chain Reaction -- methods KW - Cell Death -- drug effects KW - Caspases -- metabolism KW - Glial Fibrillary Acidic Protein -- genetics KW - Phosphopyruvate Hydratase -- metabolism KW - Cell Count -- methods KW - Microarray Analysis -- methods KW - Lasers KW - Gene Expression Regulation -- drug effects KW - Time Factors KW - Astrocytes -- pathology KW - Male KW - Lectins -- metabolism KW - Astrocytes -- metabolism KW - Neurons -- metabolism KW - Brain Injuries -- genetics KW - RNA, Messenger -- metabolism KW - Hippocampus -- metabolism KW - Hippocampus -- pathology KW - Brain Injuries -- chemically induced KW - Neurons -- pathology KW - Brain Injuries -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67523816?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurochemistry&rft.atitle=Molecular+profiles+of+mRNA+levels+in+laser+capture+microdissected+murine+hippocampal+regions+differentially+responsive+to+TMT-induced+cell+death.&rft.au=Lefebvre+d%27Hellencourt%2C+Christian%3BHarry%2C+G+Jean&rft.aulast=Lefebvre+d%27Hellencourt&rft.aufirst=Christian&rft.date=2005-04-01&rft.volume=93&rft.issue=1&rft.spage=206&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurochemistry&rft.issn=00223042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-16 N1 - Date created - 2005-03-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - On criteria for evaluating models of absolute risk. AN - 67521658; 15772102 AB - Absolute risk is the probability that an individual who is free of a given disease at an initial age, a, will develop that disease in the subsequent interval (a, t]. Absolute risk is reduced by mortality from competing risks. Models of absolute risk that depend on covariates have been used to design intervention studies, to counsel patients regarding their risks of disease and to inform clinical decisions, such as whether or not to take tamoxifen to prevent breast cancer. Several general criteria have been used to evaluate models of absolute risk, including how well the model predicts the observed numbers of events in subsets of the population ("calibration"), and "discriminatory power," measured by the concordance statistic. In this paper we review some general criteria and develop specific loss function-based criteria for two applications, namely whether or not to screen a population to select subjects for further evaluation or treatment and whether or not to use a preventive intervention that has both beneficial and adverse effects. We find that high discriminatory power is much more crucial in the screening application than in the preventive intervention application. These examples indicate that the usefulness of a general criterion such as concordance depends on the application, and that using specific loss functions can lead to more appropriate assessments. JF - Biostatistics (Oxford, England) AU - Gail, Mitchell H AU - Pfeiffer, Ruth M AD - Biostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Executive Plaza South, EPS 8032, Bethesda, MD 20892-7244, USA. gailm@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 227 EP - 239 VL - 6 IS - 2 SN - 1465-4644, 1465-4644 KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Tamoxifen KW - 094ZI81Y45 KW - Index Medicus KW - Antineoplastic Agents, Phytogenic -- adverse effects KW - Tamoxifen -- therapeutic use KW - Humans KW - Antineoplastic Agents, Phytogenic -- therapeutic use KW - Tamoxifen -- adverse effects KW - Breast Neoplasms -- prevention & control KW - Middle Aged KW - Colonoscopy -- standards KW - Colonic Neoplasms -- diagnosis KW - Female KW - Risk KW - Models, Statistical KW - Decision Making KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67521658?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Expert+review+of+anticancer+therapy&rft.atitle=Clodronate+in+the+prevention+and+treatment+of+skeletal+metastasis.&rft.au=Gulley%2C+James%3BDahut%2C+William+L&rft.aulast=Gulley&rft.aufirst=James&rft.date=2005-04-01&rft.volume=5&rft.issue=2&rft.spage=221&rft.isbn=&rft.btitle=&rft.title=Expert+review+of+anticancer+therapy&rft.issn=1744-8328&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-21 N1 - Date created - 2005-03-17 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Biostatistics. 2005 Jul;6(3):500-2; author reply 503-4 [15917375] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitrogen mineralization potentials in three tropical soils treated with biosolids. AN - 67513179; 15763098 AB - This study attained anaerobic biosolids (DS) and aerobic biosolids (MS) from the wastewater treatment plants in Kaohsiung and Taipei, Taiwan. Three tropical soils (Lt, Cp and Ca) were selected for incubation with the two biosolids at application rates of 10, 50 and 100 Mg ha(-1) for 48 weeks. This study aims to characterize the influence of the application of biosolids on the soil potential for N mineralization (N0) and also to elucidate the kinetics of N mineralization in tropical soils treated with different biosolids. Experimental results indicate that the amounts of N mineralized accumulated in the biosolids-treated soils during the incubation period tended to match the first-order kinetics calculated by the nonlinear least squares equation. The N0 values of the MS biosolids-treated soils greatly exceeded those of the DS soils. The rates of N mineralization (k) of the DS biosolids-treated soils varied greatly from 0.047 to 0.075 week(-1) and that of the MS soils varied from 0.047 to 0.105 week(-1). Little of the organic N fraction in the biosolids remained available for further mineralization following 48 weeks of incubation. Based on the demand of N uptake by vegetables grown in Taiwanese soils, the rates of biosolids application to the soils are safe, as determined by the amount of N mineralization that does not cause nitrate accumulation. JF - Chemosphere AU - Hseu, Zeng-Yei AU - Huang, Cheng-Chieh AD - Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, 1, Hseih-Fu Rd., Nei-Pu, Pingtung 91201, Taiwan, ROC. zyhseu@mail.npust.edu.tw Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 447 EP - 454 VL - 59 IS - 3 SN - 0045-6535, 0045-6535 KW - Sewage KW - 0 KW - Soil KW - Nitrogen KW - N762921K75 KW - Index Medicus KW - Taiwan KW - Analysis of Variance KW - Kinetics KW - Hydrogen-Ion Concentration KW - Least-Squares Analysis KW - Tropical Climate KW - Nitrogen -- chemistry KW - Soil -- analysis KW - Sewage -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67513179?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemosphere&rft.atitle=Nitrogen+mineralization+potentials+in+three+tropical+soils+treated+with+biosolids.&rft.au=Hseu%2C+Zeng-Yei%3BHuang%2C+Cheng-Chieh&rft.aulast=Hseu&rft.aufirst=Zeng-Yei&rft.date=2005-04-01&rft.volume=59&rft.issue=3&rft.spage=447&rft.isbn=&rft.btitle=&rft.title=Chemosphere&rft.issn=00456535&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-09 N1 - Date created - 2005-03-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Salivary hypofunction and xerostomia: diagnosis and treatment. AN - 67494214; 15755407 AB - Salivary gland hypofunction and complaints of xerostomia are common in elderly patients, irrespective of their living situation. Medication use is frequently related to dry mouth symptoms and reductions in salivary flow rates. Patients with reduced salivary flow are at increased risk for caries, oral fungal infections, swallowing problems, and diminished or altered taste. Oral health care providers should institute aggressive preventive measures and recommend palliative care for patients with significant reduction in salivary gland function. The systemic agents pilocarpine and cevimeline may help selected patients. Selective use of fluoride-releasing restorative materials and conservative treatment plans are recommended for this patient group. JF - Dental clinics of North America AU - Atkinson, Jane C AU - Grisius, Margaret AU - Massey, Ward AD - Comprehensive Care and Therapeutics, University of Maryland Dental School, 666 West Baltimore Street, 3E-32, Baltimore, MD 21201-1586, USA. jatkinso@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 309 EP - 326 VL - 49 IS - 2 SN - 0011-8532, 0011-8532 KW - Cariostatic Agents KW - 0 KW - Quinuclidines KW - Thiophenes KW - Pilocarpine KW - 01MI4Q9DI3 KW - cevimeline KW - K9V0CDQ56E KW - Fluorides KW - Q80VPU408O KW - Dentistry KW - Index Medicus KW - Thiophenes -- therapeutic use KW - Pilocarpine -- therapeutic use KW - Aged, 80 and over KW - Salivation -- drug effects KW - Humans KW - Polypharmacy KW - Cariostatic Agents -- therapeutic use KW - Quinuclidines -- therapeutic use KW - Aged KW - Sjogren's Syndrome -- complications KW - Radiotherapy -- adverse effects KW - Fluorides -- therapeutic use KW - Xerostomia -- etiology KW - Xerostomia -- prevention & control KW - Xerostomia -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67494214?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Dental+clinics+of+North+America&rft.atitle=Salivary+hypofunction+and+xerostomia%3A+diagnosis+and+treatment.&rft.au=Atkinson%2C+Jane+C%3BGrisius%2C+Margaret%3BMassey%2C+Ward&rft.aulast=Atkinson&rft.aufirst=Jane&rft.date=2005-04-01&rft.volume=49&rft.issue=2&rft.spage=309&rft.isbn=&rft.btitle=&rft.title=Dental+clinics+of+North+America&rft.issn=00118532&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-28 N1 - Date created - 2005-03-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization. AN - 67488808; 15749393 AB - 5,5-Dimethyl-1-pyrroline N-oxide (DMPO) spin trapping in conjunction with antibodies specific for the DMPO nitrone epitope was used on hydrogen peroxide-treated sperm whale and horse heart myoglobins to determine the site of protein nitrone adduct formation. The present study demonstrates that the sperm whale myoglobin tyrosyl radical, formed by hydrogen peroxide-dependent self-peroxidation, can either react with another tyrosyl radical, resulting in a dityrosine cross-linkage, or react with the spin trap DMPO to form a diamagnetic nitrone adduct. The reaction of sperm whale myoglobin with equimolar hydrogen peroxide resulted in the formation of a myoglobin dimer detectable by electrophoresis/protein staining. Addition of DMPO resulted in the trapping of the globin radical, which was detected by Western blot. The location of this adduct was demonstrated to be at tyrosine-103 by MS/MS and site-specific mutagenicity. Interestingly, formation of the myoglobin dimer, which is known to be formed primarily by cross-linkage of tyrosine-151, was inhibited by the addition of DMPO. JF - Free radical biology & medicine AU - Detweiler, Charles D AU - Lardinois, Olivier M AU - Deterding, Leesa J AU - de Montellano, Paul R Ortiz AU - Tomer, Kenneth B AU - Mason, Ronald P AD - Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0-01, Research Triangle Park, NC 27709, USA. Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 969 EP - 976 VL - 38 IS - 7 SN - 0891-5849, 0891-5849 KW - Antibodies KW - 0 KW - Cyclic N-Oxides KW - Epitopes KW - Free Radicals KW - Myoglobin KW - Nitrogen Oxides KW - Spin Labels KW - nitrones KW - Tyrosine KW - 42HK56048U KW - 5,5-dimethyl-1-pyrroline-1-oxide KW - 7170JZ1QF3 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Index Medicus KW - Free Radicals -- analysis KW - Animals KW - Antibodies -- immunology KW - Dimerization KW - Hydrogen Peroxide -- pharmacology KW - Amino Acid Sequence KW - Mutagenesis, Site-Directed KW - Cyclic N-Oxides -- analysis KW - Nitrogen Oxides -- immunology KW - Molecular Sequence Data KW - Cyclic N-Oxides -- chemistry KW - Epitopes -- immunology KW - Whales KW - Antibodies -- chemistry KW - Spin Trapping KW - Myoglobin -- immunology KW - Myoglobin -- genetics KW - Myoglobin -- chemistry KW - Tyrosine -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67488808?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Identification+of+the+myoglobin+tyrosyl+radical+by+immuno-spin+trapping+and+its+dimerization.&rft.au=Detweiler%2C+Charles+D%3BLardinois%2C+Olivier+M%3BDeterding%2C+Leesa+J%3Bde+Montellano%2C+Paul+R+Ortiz%3BTomer%2C+Kenneth+B%3BMason%2C+Ronald+P&rft.aulast=Detweiler&rft.aufirst=Charles&rft.date=2005-04-01&rft.volume=38&rft.issue=7&rft.spage=969&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-03-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cytotoxicity of RH1: NAD(P)H:quinone acceptor oxidoreductase (NQO1)-independent oxidative stress and apoptosis induction. AN - 67486176; 15746574 AB - The elevated expression of the flavoprotein NAD(P)H:quinone acceptor oxidoreductase (NQO1) (EC 1.6.99.2) in many human solid tumors, along with its ability to activate quinone-based anticancer agents, makes it an excellent target for enzyme-directed drug development. Previous studies have shown a significant statistical correlation between NQO1 enzymatic activity and the cytotoxicity of certain antitumor quinones. RH1 [2,5-diaziridinyl-3-(hydroxymethyl)-6-methyl-1,4-benzoquinone], presently in late preclinical and entering early clinical development, has been previously considered to be an excellent substrate for activation by NQO1. In this study we investigate the cytotoxicity of RH1 in cell lines selected from the NCI's 60 tumor cell line panel, expressing varying levels of NQO1 activity. Exposure time- and concentration-dependent cytotoxicity was seen, apparently independent from levels of NQO1 activity in these cells. Furthermore, the NQO1 inhibitor dicoumarol had no impact on the sensitivity profiles of RH1 response. The HL-60 myeloid leukemia cells, which do not have detectable NQO1 activity, were further investigated. RH1 treatment of HL-60 cells generated high levels of free radicals, which was accompanied by robust redox cycling, oxygen consumption and induction of apoptosis. These results are in agreement with previous data suggesting that, in addition to its activation by NQO1, RH1-induced cytotoxicity might involve alternative pathways for activation of this compound. Furthermore, the high cytotoxicity of RH1 in the leukemia/lymphoma subpanel of the NCI in vitro cell line screen would suggest an empirical rationale for the utilization of this compound in the treatment of these malignancies. JF - Anti-cancer drugs AU - Tudor, Gabriela AU - Alley, Mike AU - Nelson, Christopher M AU - Huang, Ruili AU - Covell, David G AU - Gutierrez, Peter AU - Sausville, Edward A AD - Science Applications International Corp., National Cancer Institute, Frederick, MD, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 381 EP - 391 VL - 16 IS - 4 SN - 0959-4973, 0959-4973 KW - 2,5-diaziridinyl-3-(hydroxymethyl)-6-methyl-1,4-benzoquinone KW - 0 KW - Aziridines KW - Benzoquinones KW - Free Radicals KW - NAD(P)H Dehydrogenase (Quinone) KW - EC 1.6.5.2 KW - NQO1 protein, human KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Oxidation-Reduction KW - HL-60 Cells -- metabolism KW - HL-60 Cells -- drug effects KW - Transfection KW - Dose-Response Relationship, Drug KW - Oxygen -- metabolism KW - Humans KW - Time Factors KW - Free Radicals -- metabolism KW - Benzoquinones -- toxicity KW - Aziridines -- toxicity KW - Apoptosis -- drug effects KW - Oxidative Stress -- drug effects KW - NAD(P)H Dehydrogenase (Quinone) -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67486176?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anti-cancer+drugs&rft.atitle=Cytotoxicity+of+RH1%3A+NAD%28P%29H%3Aquinone+acceptor+oxidoreductase+%28NQO1%29-independent+oxidative+stress+and+apoptosis+induction.&rft.au=Tudor%2C+Gabriela%3BAlley%2C+Mike%3BNelson%2C+Christopher+M%3BHuang%2C+Ruili%3BCovell%2C+David+G%3BGutierrez%2C+Peter%3BSausville%2C+Edward+A&rft.aulast=Tudor&rft.aufirst=Gabriela&rft.date=2005-04-01&rft.volume=16&rft.issue=4&rft.spage=381&rft.isbn=&rft.btitle=&rft.title=Anti-cancer+drugs&rft.issn=09594973&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetics of individual differences in bitter taste perception: lessons from the PTC gene. AN - 67470059; 15733260 AB - The ability or inability to taste the compound phenylthiocarbamide (PTC) is a classic inherited trait in humans and has been the subject of genetic and anthropological studies for over 70 years. This trait has also been shown to correlate with a number of dietary preferences and thus may have important implications for human health. The recent identification of the gene that underlies this phenotype has produced several surprising findings. This gene is a member of the T2R family of bitter taste receptor genes. It exists in seven different allelic forms, although only two of these, designated the major taster and major non-taster forms, exist at high frequency outside sub-Saharan Africa. The non-taster allele resides on a small chromosomal region identical by descent, indicating that non-tasters are descended from an ancient founder individual, and consistent with an origin of the non-taster allele preceding the emergence of modern humans out of Africa. The two major forms differ from each other at three amino acid positions, and both alleles have been maintained at high frequency by balancing natural selection, suggesting that the non-taster allele serves some function. We hypothesize that this function is to serve as a receptor for another, as yet unidentified toxic bitter substance. At least some of the remaining five haplotypes appear to confer intermediate sensitivity to PTC, suggesting future detailed studies of the relationships between receptor structure and taste function. JF - Clinical genetics AU - Kim, U K AU - Drayna, D AD - National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 275 EP - 280 VL - 67 IS - 4 SN - 0009-9163, 0009-9163 KW - Receptors, Cell Surface KW - 0 KW - Receptors, G-Protein-Coupled KW - taste receptors, type 2 KW - Phenylthiourea KW - 6F82C6Q54C KW - Index Medicus KW - Phenotype KW - Genotype KW - Genetic Linkage KW - Alleles KW - Haplotypes KW - Genetics, Population KW - African Continental Ancestry Group -- genetics KW - Humans KW - Founder Effect KW - Evolution, Molecular KW - Taste -- genetics KW - Receptors, Cell Surface -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67470059?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+genetics&rft.atitle=Genetics+of+individual+differences+in+bitter+taste+perception%3A+lessons+from+the+PTC+gene.&rft.au=Kim%2C+U+K%3BDrayna%2C+D&rft.aulast=Kim&rft.aufirst=U&rft.date=2005-04-01&rft.volume=67&rft.issue=4&rft.spage=275&rft.isbn=&rft.btitle=&rft.title=Clinical+genetics&rft.issn=00099163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-16 N1 - Date created - 2005-02-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: Clin Genet. 2005 Jun;67(6):534 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tissue homeostasis and the control of the neoplastic phenotype in epithelial cancers. AN - 67370199; 15652452 AB - Neoplastic cells at various stages of tumor progression may remain dormant for many years. The suppression of the neoplastic phenotype and tumor outgrowth depends on close contact of neoplastic cells with surrounding normal cells. This review examines the nature of these contacts primarily in models for skin cancer induction. Junctional complexes, membrane associated growth factors and their receptors, and paracrine mechanisms likely contribute to this state of tumor cell dormancy. Understanding these mechanisms will be important in primary cancer prevention and for counteracting recurrences in cancer survivors. JF - Seminars in cancer biology AU - Glick, Adam B AU - Yuspa, Stuart H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, MSC-4255, Building 37, Room 4068A1, Bethesda, MD 20892-4255, USA. Y1 - 2005/04// PY - 2005 DA - April 2005 SP - 75 EP - 83 VL - 15 IS - 2 SN - 1044-579X, 1044-579X KW - Index Medicus KW - Phenotype KW - Animals KW - Gap Junctions -- physiology KW - Cell Communication KW - Homeostasis KW - Carcinoma -- etiology KW - Carcinoma -- pathology KW - Skin Neoplasms -- etiology KW - Skin Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67370199?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Seminars+in+cancer+biology&rft.atitle=Tissue+homeostasis+and+the+control+of+the+neoplastic+phenotype+in+epithelial+cancers.&rft.au=Glick%2C+Adam+B%3BYuspa%2C+Stuart+H&rft.aulast=Glick&rft.aufirst=Adam&rft.date=2005-04-01&rft.volume=15&rft.issue=2&rft.spage=75&rft.isbn=&rft.btitle=&rft.title=Seminars+in+cancer+biology&rft.issn=1044579X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-14 N1 - Date created - 2005-01-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Quantifying the Magnitude of Baseline Covariate Imbalances Resulting from Selection Bias in Randomized Clinical Trials AN - 21125513; 11157936 AB - Selection bias is most common in observational studies, when patients select their own treatments or treatments are assigned based on patient characteristics, such as disease severity. This first-order selection bias, as we call it, is eliminated by randomization, but there is residual selection bias that may occur even in randomized trials which occurs when, subconsciously or otherwise, an investigator uses advance knowledge of upcoming treatment allocations as the basis for deciding whom to enroll. For example, patients more likely to respond may be preferentially enrolled when the active treatment is due to be allocated, and patients less likely to respond may be enrolled when the control group is due to be allocated. If the upcoming allocations can be observed in their entirety, then we will call the resulting selection bias second-order selection bias. Allocation concealment minimizes the ability to observe upcoming allocations, yet upcoming allocations may still be predicted (imperfectly), or even determined with certainty, if at least some of the previous allocations are known, and if restrictions (such as randomized blocks) were placed on the randomization. This mechanism, based on prediction but not observation of upcoming allocations, is the third-order selection bias that is controlled by perfectly successful masking, but without perfect masking is not controlled even by the combination of advance randomization and allocation concealment. Our purpose is to quantify the magnitude of baseline imbalance that can result from third-order selection bias when the randomized block procedure is used. The smaller the block sizes, the more accurately one can predict future treatment assignments in the same block as known previous assignments, so this magnitude will depend on the block size, as well as on the level of certainty about upcoming allocations required to bias the patient selection. We find that a binary covariate can, on average, be up to 50% unbalanced by third-order selection bias. JF - Biometrical Journal AU - Berger, Vance W AD - National Cancer Institute, University of Maryland Baltimore County, Biometry Research Group, National Cancer Institute, Executive Plaza North, Suite 3131, 6130 Executive Boulevard, MSC 7354, Bethesda, MD 20892-7354, USA, vb78c@nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 119 EP - 127 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 47 IS - 2 SN - 0323-3847, 0323-3847 KW - Biotechnology and Bioengineering Abstracts KW - Statistics KW - Biometrics KW - Clinical trials KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21125513?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biometrical+Journal&rft.atitle=Quantifying+the+Magnitude+of+Baseline+Covariate+Imbalances+Resulting+from+Selection+Bias+in+Randomized+Clinical+Trials&rft.au=Berger%2C+Vance+W&rft.aulast=Berger&rft.aufirst=Vance&rft.date=2005-04-01&rft.volume=47&rft.issue=2&rft.spage=119&rft.isbn=&rft.btitle=&rft.title=Biometrical+Journal&rft.issn=03233847&rft_id=info:doi/10.1002%2Fbimj.200410106 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Clinical trials; Biometrics; Statistics DO - http://dx.doi.org/10.1002/bimj.200410106 ER - TY - JOUR T1 - Nonparametric Adjustment Techniques for Binary Covariates AN - 21095186; 11132754 AB - Though a variety of reasons are often articulated for adjusting analyses for covariates, these reasons often fall into one of two general objectives, specifically to increase precision or to decrease bias. In practice, one does not generally choose between these objectives, because the methods that address one tend to address the other, as well. Because of this, no distinction is made in the methods used to correct for a baseline imbalance with respect to a prognostic covariate versus to ensure a fair comparison across treatment groups by making the comparisons within the levels of a prognostic covariate. Yet the literal translation of these two uses of covariate adjustment will lead to two distinct adjustment methods. We illustrate this divergence in the simplest case of a single binary covariate, a binary outcome, and two treatments, and we note that it is possible to combine the two approaches to derive yet a third approach. Each of these approaches is nonparametric and exact, and so it is the precise reason for adjusting that should dictate which would be used in any given situation. JF - Biometrical Journal AU - Berger, Vance W AD - National Cancer Institute and University of Maryland Baltimore County, EPN 3131, 6130 Executive Boulevard, MSC-7354, Bethesda, MD 20892-7354, USA, vb78c@nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 199 EP - 205 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 47 IS - 2 SN - 0323-3847, 0323-3847 KW - Biotechnology and Bioengineering Abstracts KW - Translation KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21095186?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biometrical+Journal&rft.atitle=Nonparametric+Adjustment+Techniques+for+Binary+Covariates&rft.au=Berger%2C+Vance+W&rft.aulast=Berger&rft.aufirst=Vance&rft.date=2005-04-01&rft.volume=47&rft.issue=2&rft.spage=199&rft.isbn=&rft.btitle=&rft.title=Biometrical+Journal&rft.issn=03233847&rft_id=info:doi/10.1002%2Fbimj.200410100 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Translation DO - http://dx.doi.org/10.1002/bimj.200410100 ER - TY - JOUR T1 - Power and Sample Size Calculation of Comparative Diagnostic Accuracy Studies with Multiple Correlated Test Results AN - 21075080; 11132748 AB - We consider the power and sample size calculation of diagnostic studies with normally distributed multiple correlated test results. We derive test statistics and obtain power and sample size formulas. The methods are illustrated using an example of comparison of CT and PET scanner for detecting extra-hepatic disease for colorectal cancer. JF - Biometrical Journal AU - Liu, Aiyi AU - Schisterman, Enrique F AU - Mazumdar, Madhu AU - Hu, Jiang AD - Division of Epidemiology, Statistics and Prevention Research, National Institute of Child Health and Human Development, Department of Health and Human Services, 6100 Executive Blvd., Rockville, MD 20852, USA, liua@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 140 EP - 150 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 USA VL - 47 IS - 2 SN - 0323-3847, 0323-3847 KW - Biotechnology and Bioengineering Abstracts KW - Statistics KW - Colorectal cancer KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21075080?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biometrical+Journal&rft.atitle=Power+and+Sample+Size+Calculation+of+Comparative+Diagnostic+Accuracy+Studies+with+Multiple+Correlated+Test+Results&rft.au=Liu%2C+Aiyi%3BSchisterman%2C+Enrique+F%3BMazumdar%2C+Madhu%3BHu%2C+Jiang&rft.aulast=Liu&rft.aufirst=Aiyi&rft.date=2005-04-01&rft.volume=47&rft.issue=2&rft.spage=140&rft.isbn=&rft.btitle=&rft.title=Biometrical+Journal&rft.issn=03233847&rft_id=info:doi/10.1002%2Fbimj.200410094 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Colorectal cancer; Statistics DO - http://dx.doi.org/10.1002/bimj.200410094 ER - TY - JOUR T1 - Differential Expression of Cytokines and Chemokines in Human Monocytes Induced by Lipid Formulations of Amphotericin B AN - 20714068; 6243586 AB - The immunomodulatory effects of liposomal amphotericin B (LAMB), amphotericin B lipid complex (ABLC), and amphotericin B colloidal dispersion (ABCD) on mRNA and protein profiles of five cytokines and chemokines expressed by human monocyte-enriched mononuclear leukocytes (MNCs) were comprehensively evaluated by semiquantitative reverse transcription-PCR and enzyme-linked immunosorbent assays; they were compared to those of deoxycholate amphotericin B (DAMB). mRNAs of interleukin-1 beta (IL-1 beta ), IL-1 receptor antagonist (IL-1ra), tumor necrosis factor alpha (TNF- alpha ), monocyte chemotactic protein 1 (MCP-1), and macrophage inflammatory protein 1 beta (MIP-1 beta ) were assessed after treatment of MNCs with each drug for 0.5, 2, 6, and 22 h. The cytokine protein profiles were obtained after incubation of MNCs with the drugs for 2 h (TNF- alpha ) or 6 h (all the others). In the mRNA studies, DAMB resulted in an early increase of inflammatory cytokines or chemokines IL-1 beta , TNF- alpha , MCP-1, and MIP-1 beta (2 to 6 h) and in a late increase of anti-inflammatory IL-1ra (22 h). ABCD showed a general similar trend of inflammatory gene up-regulation. LAMB and ABLC decreased or did not affect IL-1 beta and TNF- alpha , whereas ABLC additionally decreased MIP-1 beta . In protein measurement studies, DAMB and ABCD up-regulated production of IL-1 beta (P < 0.05), decreased the IL-1ra/IL-1 beta ratio, and up-regulated the production of MCP-1 and MIP-1 beta . In comparison, LAMB and ABLC down-regulated or did not affect the production of these cytokines/chemokines compared to untreated MNCs; furthermore, ABLC tended to increase the IL-1ra/IL-1 beta ratio. These studies demonstrate that amphotericin B formulations differentially affect gene expression and release of an array of proinflammatory and anti-inflammatory cytokines that potentially may explain the differences in infusion-related reactions and dose-dependent nephrotoxicity as well as modulation of the host immune response to invasive fungal infections. JF - Antimicrobial Agents & Chemotherapy AU - Simitsopoulou, M AU - Roilides, E AU - Dotis, J AU - Dalakiouridou, M AU - Dudkova, F AU - Andreadou, E AU - Walsh, T J AD - Laboratory of Infectious Diseases, 3rd Department of Pediatrics, School of Medicine, Aristotle University of Thessaloniki. Laboratory of Medical Biotechnology, Department of Medical Laboratories, Technological Educational Institute, Thessaloniki, Greece. Immunocompromised Host Section, National Cancer Institute, Bethesda, Maryland Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1397 EP - 1403 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 49 IS - 4 SN - 0066-4804, 0066-4804 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Microbiology Abstracts A: Industrial & Applied Microbiology; Immunology Abstracts KW - Leukocytes (mononuclear) KW - Amphotericin B KW - Chemokines KW - Enzyme-linked immunosorbent assay KW - Monocyte chemoattractant protein 1 KW - Lipids KW - Interleukin 1 KW - Transcription KW - Infection KW - Immunomodulation KW - Interleukin 1 receptor antagonist KW - Inflammation KW - Antimicrobial agents KW - Gene expression KW - Cytokines KW - Immune response KW - Monocytes KW - Tumor necrosis factor- alpha KW - Macrophage inflammatory protein KW - Drugs KW - A 01340:Antibiotics & Antimicrobials KW - F 06955:Immunomodulation & Immunopharmacology KW - K 03310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20714068?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+Gene+Therapy&rft.atitle=Transfer+of+a+TCR+Gene+Derived+from+a+Patient+with+a+Marked+Antitumor+Response+Conveys+Highly+Active+T-Cell+Effector+Functions&rft.au=Hughes%3BYu%2C+YYL%3BDudley%2C+ME%3BZheng%2C+Z%3BRobbins%2C+P+F%3BLi%2C+Y%3BWunderlich%2C+J%3BHawley%2C+R+G%3BMoayeri%2C+M%3BRosenberg%2C+SA%3BMorgan%2C+R+A&rft.aulast=Hughes&rft.aufirst=&rft.date=2005-04-01&rft.volume=16&rft.issue=4&rft.spage=457&rft.isbn=&rft.btitle=&rft.title=Human+Gene+Therapy&rft.issn=10430342&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Amphotericin B; Leukocytes (mononuclear); Enzyme-linked immunosorbent assay; Chemokines; Monocyte chemoattractant protein 1; Lipids; Interleukin 1; Transcription; Infection; Immunomodulation; Interleukin 1 receptor antagonist; Antimicrobial agents; Inflammation; Gene expression; Cytokines; Tumor necrosis factor- alpha; Monocytes; Immune response; Macrophage inflammatory protein; Drugs ER - TY - JOUR T1 - Anticancer metal compounds in NCI's tumor-screening database: putative mode of action AN - 20237971; 6201941 AB - Clustering analysis of tumor cell cytotoxicity profiles for the National Cancer Institute (NCI)'s open compound repository has been used to catalog over 1100 metal or metalloid containing compounds with potential anticancer activity. The molecular features and corresponding reactivity of these compounds have been analyzed in terms of properties of their metals, their associated organic components (ligands) and their capacity to inhibit tumor cell growth. Cytotoxic responses are influenced by both the identity of the metal and the properties of its coordination ligand, with clear associations between structural similarities and cytotoxicity. Assignments of mechanisms of action (MOAs) for these compounds could be segregated into four broad response classes according to preference for binding to biological sulfhydryl groups, chelation, generation of reactive oxygen species (ROS), and production of lipophilic ions. Correlations between specific cytotoxic responses and differential gene expression profiles within the NCI's tumor cell panel serve as a validation for candidate biological targets and putative MOA classes. In addition, specific sensitivity toward subsets of metal containing agents has been found for certain tumor cell panels. Taken together, our results expand the knowledge base available for evaluating, designing and developing new metal-based anticancer drugs that may provide the basis for target-specific therapeutics. JF - Biochemical Pharmacology AU - Huang, R AU - Wallqvist, A AU - Covell, D G AD - National Cancer Institute at Frederick, Developmental Therapeutics Program, Screening Technologies Branch, Laboratory of Computational Technologies, Frederick, MD 21702, USA, covell@mail.ncifcrf.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1009 EP - 1039 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 69 IS - 7 SN - 0006-2952, 0006-2952 KW - Biotechnology and Bioengineering Abstracts KW - Data mining KW - NCI tumor screen KW - Metal compounds KW - Mechanism of action KW - Drug discovery KW - Cancer KW - Ions KW - Metals KW - Catalogs KW - Chelation KW - Sulfhydryl groups KW - Antitumor agents KW - Tumor cells KW - Lipophilic KW - Gene expression KW - Databases KW - Cytotoxicity KW - Reactive oxygen species KW - Antitumor activity KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20237971?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+Pharmacology&rft.atitle=Anticancer+metal+compounds+in+NCI%27s+tumor-screening+database%3A+putative+mode+of+action&rft.au=Huang%2C+R%3BWallqvist%2C+A%3BCovell%2C+D+G&rft.aulast=Huang&rft.aufirst=R&rft.date=2005-04-01&rft.volume=69&rft.issue=7&rft.spage=1009&rft.isbn=&rft.btitle=&rft.title=Biochemical+Pharmacology&rft.issn=00062952&rft_id=info:doi/10.1016%2Fj.bcp.2005.01.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Metals; Ions; Catalogs; Chelation; Sulfhydryl groups; Tumor cells; Antitumor agents; Lipophilic; Gene expression; Databases; Cytotoxicity; Reactive oxygen species; Antitumor activity DO - http://dx.doi.org/10.1016/j.bcp.2005.01.001 ER - TY - JOUR T1 - The Rate of Serious Bacterial Infections Among HIV-Infected Children With Immune Reconstitution Who Have Discontinued Opportunistic Infection Prophylaxis AN - 20193653; 6267834 AB - OBJECTIVE: Receipt of highly active antiretroviral therapy is associated with a decrease in the incidence of opportunistic infections (OIs) among HIV-infected adults. The goal of Pediatric AIDS Clinical Trials Group protocol 1008 was to evaluate prospectively the incidence of serious bacterial infections (SBIs) and other OIs after discontinuation of OI and/or Pneumocystis jiroveci pneumonia (PCP) prophylaxis among HIV-infected pediatric subjects who experienced immune reconstitution while receiving stable antiretroviral therapy. METHODS: HIV-infected children and adolescents, 2 to 21 years of age, who had received OI and/or PCP prophylaxis for greater than or equal to 6 months were enrolled if they had sustained responses (>16 weeks before study entry) to antiretroviral therapy, with CD4 super(+) cell percentages of greater than or equal to 20% for patients >6 years of age or greater than or equal to 25% for patients 2 to 6 years of age. Prophylaxis was discontinued at entry. To identify whether any correlation existed between functional immune reconstitution and protection from OIs, subjects were immunized with the hepatitis A virus vaccine. The association between the humoral immune response and the likelihood of developing an OI was evaluated. RESULTS: A total of 235 HIV-infected subjects from 43 participating sites had a median follow-up period of 132 weeks, yielding 547 person-years of observation. Twenty SBIs were observed among 19 subjects, resulting in an incidence rate of 3.66 SBIs per 100 person-years (95% confidence interval: 2.24-5.66 SBIs per 100 person-years). Sixteen of the events were presumed bacterial pneumonia, with 4 proven SBIs. One participant experienced 2 separate pneumonia episodes, of presumed bacterial cause. Ten subjects who developed SBIs had baseline CD4 super(+) cell counts of greater than or equal to 750 cells per mm super(3), and 15 had CD4 super(+) cell percentages of greater than or equal to 25% at the time of their SBIs. Two subjects died as a result of non-SBI-related causes. There were no statistically significant differences in changes over time in CD4 super(+) cell counts or CD4 super(+) cell percentages between subjects who experienced primary end points and those who did not. There was no evidence that baseline protease inhibitor use, gender, race/ethnicity, age, or CD4 super(+) cell count or percentage affected the time to development of a SBI. CONCLUSIONS: OI or PCP prophylaxis can be withdrawn safely for HIV-infected pediatric patients who experience CD4 super(+) cell recovery while receiving stable antiretroviral therapy. More studies are needed to assess the association between antibody responses to neoantigens and the development of SBIs. JF - Pediatrics AU - Nachman, Sharon AU - Gona, Philimon AU - Dankner, Wayne AU - Weinberg, Adrianna AU - Yogev, Ram AU - Gershon, Anne AU - Rathore, Mobeen AU - Read, Jennifer S AU - Huang, Sharon AU - Elgie, Carol AU - Hudgens, Kim AU - Hughes, Walter AD - Stony Brook University, State University of New York, Stony Brook, New York. Center for Biostatistics in AIDS Research, Harvard School of Public Health, Boston, Massachusetts. Duke University Medical Center, Durham, North Carolina. University of Colorado Health Sciences Center, Denver, Colorado. Northwestern University, Chicago, Illinois. Columbia Presbyterian Medical Center, New York, New York. University of Florida Health Science Center, Jacksonville, FL. National Institute of Child Health and Human Development, Bethesda, Maryland. Frontier Science and Technology Research Foundation, Amherst, New York. Social and Scientific Systems, Silver Spring, Maryland. St Jude Children's Research Hospital, Memphis, Tennessee Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - e488 EP - e494 PB - American Academy of Pediatrics, 141 Northwest Point Blvd. Elk Grove Village IL 60007-1098 USA, [mailto:journals@aap.org], [URL:http://www.aap.org] VL - 115 IS - 4 SN - 0031-4005, 0031-4005 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Acquired immune deficiency syndrome KW - Age KW - Hepatitis A virus KW - Pneumocystis KW - Statistical analysis KW - Clinical trials KW - Immune reconstitution KW - CD4 antigen KW - Immune response (humoral) KW - Ethnic groups KW - Races KW - Bacteria KW - Pediatrics KW - Adolescence KW - Proteinase inhibitors KW - antiretroviral therapy KW - Children KW - Opportunist infection KW - Antibodies KW - Human immunodeficiency virus KW - highly active antiretroviral therapy KW - Prophylaxis KW - Vaccines KW - Pneumonia KW - A 01340:Antibiotics & Antimicrobials KW - V 22360:AIDS and HIV KW - F 06905:Vaccines KW - J 02350:Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20193653?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatrics&rft.atitle=The+Rate+of+Serious+Bacterial+Infections+Among+HIV-Infected+Children+With+Immune+Reconstitution+Who+Have+Discontinued+Opportunistic+Infection+Prophylaxis&rft.au=Nachman%2C+Sharon%3BGona%2C+Philimon%3BDankner%2C+Wayne%3BWeinberg%2C+Adrianna%3BYogev%2C+Ram%3BGershon%2C+Anne%3BRathore%2C+Mobeen%3BRead%2C+Jennifer+S%3BHuang%2C+Sharon%3BElgie%2C+Carol%3BHudgens%2C+Kim%3BHughes%2C+Walter&rft.aulast=Nachman&rft.aufirst=Sharon&rft.date=2005-04-01&rft.volume=115&rft.issue=4&rft.spage=e488&rft.isbn=&rft.btitle=&rft.title=Pediatrics&rft.issn=00314005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Age; Acquired immune deficiency syndrome; Pediatrics; Adolescence; antiretroviral therapy; Proteinase inhibitors; Statistical analysis; Children; Clinical trials; Opportunist infection; Immune reconstitution; Antibodies; CD4 antigen; highly active antiretroviral therapy; Prophylaxis; Vaccines; Immune response (humoral); Races; Ethnic groups; Pneumonia; Bacteria; Human immunodeficiency virus; Hepatitis A virus; Pneumocystis ER - TY - JOUR T1 - Bacterial Small RNA Regulators AN - 19779564; 7373930 AB - Small regulatory RNAs can modify the activity of proteins and the stability and translation of mRNAs. They have now been found in a wide range of organisms, and can play previously unsuspected critical regulatory roles. The bacterial small RNAs include two major classes. The largest family (with at least 20 members in Escherichia coli K12) acts by basepairing with target mRNAs to modify mRNA translation or stability; this class of RNAs also uses an RNA chaperone protein, Hfq. DsrA is the best-studied example of this family of RNAs. It has been shown to positively regulate translation of the transcription factor RpoS by opening an inhibitory hairpin in the mRNA, and to negatively regulate translation of hns by pairing just beyond the translation initiation codon. The class of RNAs that modify activity of proteins is exemplified by CsrB and CsrC of E. coli, two RNAs that bind to and inhibit CsrA, a protein translational regulator. Homologs of CsrA and related regulatory RNAs have been implicated in the regulation of gluconeogenesis, biofilm formation, and virulence factor expression in plant and human pathogens. JF - Critical Reviews in Biochemistry and Molecular Biology AU - Majdalani, N AU - Vanderpool, C K AU - Gottesman, S AD - Bldg. 37, Rm. 5132, National Cancer Institute, Bethesda, MD 20892, USA, susang@helix.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 93 EP - 113 VL - 40 IS - 2 SN - 1040-9238, 1040-9238 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - Translation KW - mRNA stability KW - virulence factors KW - Translation initiation KW - Transcription factors KW - Gluconeogenesis KW - Escherichia coli KW - Codons KW - Chaperones KW - Pathogens KW - Biofilms KW - A 01360:Plant Diseases KW - J 02320:Cell Biology KW - N 14830:RNA UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19779564?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Critical+Reviews+in+Biochemistry+and+Molecular+Biology&rft.atitle=Bacterial+Small+RNA+Regulators&rft.au=Majdalani%2C+N%3BVanderpool%2C+C+K%3BGottesman%2C+S&rft.aulast=Majdalani&rft.aufirst=N&rft.date=2005-04-01&rft.volume=40&rft.issue=2&rft.spage=93&rft.isbn=&rft.btitle=&rft.title=Critical+Reviews+in+Biochemistry+and+Molecular+Biology&rft.issn=10409238&rft_id=info:doi/10.1080%2F10409230590918702 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-05-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Translation; mRNA stability; virulence factors; Translation initiation; Transcription factors; Gluconeogenesis; Codons; Chaperones; Biofilms; Pathogens; Escherichia coli DO - http://dx.doi.org/10.1080/10409230590918702 ER - TY - JOUR T1 - Data and Safety Monitoring in Clinical Research: A National Institute of Neurologic Disorders and Stroke Perspective AN - 19520350; 7231945 AB - The National Institute of Neurologic Disorders and Stroke supports a broad spectrum of research in the diagnosis and treatment of neurologic disease. Emergency medicine is increasingly involved in clinical research for patients with neurologic emergencies. Independent data and safety monitoring are critical components of clinical trials to ensure the protection of patients and the scientific integrity of the research. We review National Institute of Neurologic Disorders and Stroke principles of data and safety monitoring and provide examples to illustrate key concepts. JF - Annals of Emergency Medicine AU - Conwit, R A AU - Hart, R G AU - Moy, C S AU - Marler, J R AD - National Institute of Neurological Disorders and Stroke, National Institutes of Health, Neuroscience Center, Bethesda, MD, USA Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 388 EP - 392 VL - 45 IS - 4 SN - 0196-0644, 0196-0644 KW - neurological disorders KW - Health & Safety Science Abstracts KW - Reviews KW - clinical trials KW - Research programs KW - H 13000:Medical Safety UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19520350?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+Emergency+Medicine&rft.atitle=Data+and+Safety+Monitoring+in+Clinical+Research%3A+A+National+Institute+of+Neurologic+Disorders+and+Stroke+Perspective&rft.au=Conwit%2C+R+A%3BHart%2C+R+G%3BMoy%2C+C+S%3BMarler%2C+J+R&rft.aulast=Conwit&rft.aufirst=R&rft.date=2005-04-01&rft.volume=45&rft.issue=4&rft.spage=388&rft.isbn=&rft.btitle=&rft.title=Annals+of+Emergency+Medicine&rft.issn=01960644&rft_id=info:doi/10.1016%2Fj.annemergmed.2004.08.006 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Reviews; clinical trials; Research programs DO - http://dx.doi.org/10.1016/j.annemergmed.2004.08.006 ER - TY - JOUR T1 - The prevalence and clinical significance of amniotic fluid 'sludge' in patients with preterm labor and intact membranes AN - 19432416; 6786691 AB - Objective: To determine the prevalence and clinical significance of amniotic fluid (AF) 'sludge' observed during transvaginal ultrasound examination of the cervix in patients with preterm labor and intact membranes, and in those with uncomplicated pregnancies. Methods: This retrospective study included patients with preterm labor and intact membranes (n = 84) and those with uncomplicated term pregnancies (n = 298). The outcome variables included the occurrence of documented microbial invasion of the amniotic cavity (MIAC), histological chorioamnionitis, examination-to-delivery interval, admission to the neonatal intensive care unit (NICU), a composite neonatal morbidity, perinatal death, and delivery within 48 h, 7 days, and < 35 weeks and < 32 weeks. Statistical analysis included Chi-square test, stepwise logistic regression analysis and survival analysis. Results: The prevalence of AF 'sludge' was 1% (3/298) in patients with uncomplicated term pregnancies and 22.6% (19/84) in those with preterm labor and intact membranes. Among patients with preterm labor and intact membranes: (1) cervical length less than or equal to 15 mm was present in 58.3% (49/84) of the patients; (2) the prevalence of MIAC and histological chorioamnionitis was 12.1% (7/58) and 32.9% (25/76), respectively; (3) the rate of spontaneous preterm delivery within 48 h, 7 days, and < 32 weeks and < 35 weeks of gestation was 13.6% (8/59), 28.8% (17/59), 39.5% (17/43) and 50.8% (30/59), respectively; (4) patients with AF 'sludge' had a higher frequency of positive AF cultures [33.3% (6/18) vs. 2.5% (1/40), P = 0.003] and histological chorioamnionitis [77.8% (14/18) vs. 19% (11/58), P < 0.001] than those without AF 'sludge'; (5) a higher proportion of neonates born to patients with AF 'sludge' was admitted to the NICU [64.3% (9/14) vs. 12.9% (8/62), P < 0.01], had a composite neonatal morbidity [36.8% (7/19) vs. 13.8% (9/65), P = 0.04] and died in the perinatal period [36.8% (7/19) vs. 4.6% (3/65), P = 0.001] than those born to women without 'sludge'; (6) a higher proportion of patients with AF 'sludge' had spontaneous delivery within 48 h [42.9% (6/14) vs. 4.4% (2/45), P = 0.001], within 7 days [71.4% (10/14) vs. 15.6% (7/45), P < 0.001], < 32 weeks [75% (9/12) vs. 25.8% (8/31), P = 0.005] and < 35 weeks [92.9% (13/14) vs. 37.8% (17/45), P < 0.001] than those without AF 'sludge'; and (7) patients with AF 'sludge' had a shorter examination-to-delivery interval than those without AF 'sludge' [AF 'sludge' median, 1 (IQR, 1-5) days vs. no AF 'sludge' median, 33 (IQR, 18-58) days; P < 0.001]. Conclusion: The presence of AF 'sludge' in patients with preterm labor and intact membranes is a risk factor for MIAC, histological chorioamnionitis and impending preterm delivery. JF - Ultrasound in Obstetrics and Gynecology AU - Espinoza, J AU - Goncalves, L F AU - Romero, R AU - Nien, J K AU - Stites, S AU - Kim, Y M AU - Hassan, S AU - Gomez, R AU - Yoon, B H AU - Chaiworapongsa, T AU - Lee, W AU - Mazor, M AD - Perinatology Research Branch, NICHD/NIH/DHHS, 4707 St Antoine Boulevard, Detroit, MI 48201, USA, warfiela@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 346 EP - 352 PB - John Wiley & Sons, Ltd. VL - 25 IS - 4 SN - 0960-7692, 0960-7692 KW - Biotechnology and Bioengineering Abstracts KW - Amniotic fluid KW - Gynecology KW - Sludges KW - Statistical analysis KW - Survival KW - Morbidity KW - Pregnancy KW - Intensive care units KW - Risk factors KW - Gestation KW - Regression analysis KW - Chorioamnionitis KW - Neonates KW - Cervix KW - Obstetrics KW - Ultrasound KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19432416?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Ultrasound+in+Obstetrics+and+Gynecology&rft.atitle=The+prevalence+and+clinical+significance+of+amniotic+fluid+%27sludge%27+in+patients+with+preterm+labor+and+intact+membranes&rft.au=Espinoza%2C+J%3BGoncalves%2C+L+F%3BRomero%2C+R%3BNien%2C+J+K%3BStites%2C+S%3BKim%2C+Y+M%3BHassan%2C+S%3BGomez%2C+R%3BYoon%2C+B+H%3BChaiworapongsa%2C+T%3BLee%2C+W%3BMazor%2C+M&rft.aulast=Espinoza&rft.aufirst=J&rft.date=2005-04-01&rft.volume=25&rft.issue=4&rft.spage=346&rft.isbn=&rft.btitle=&rft.title=Ultrasound+in+Obstetrics+and+Gynecology&rft.issn=09607692&rft_id=info:doi/10.1002%2Fuog.1871 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Sludges; Neonates; Chorioamnionitis; Pregnancy; Ultrasound; Morbidity; Amniotic fluid; Survival; Gynecology; Gestation; Risk factors; Obstetrics; Statistical analysis; Intensive care units; Regression analysis; Cervix DO - http://dx.doi.org/10.1002/uog.1871 ER - TY - JOUR T1 - Cross-Resistance of Escherichia coli RNA Polymerases Conferring Rifampin Resistance to Different Antibiotics AN - 17872740; 6245818 AB - In this study we further defined the rifampin-binding sites in Escherichia coli RNA polymerase (RNAP) and determined the relationship between rifampin-binding sites and the binding sites of other antibiotics, including two rifamycin derivatives, rifabutin and rifapentine, and streptolydigin and sorangicin A, which are unrelated to rifampin, using a purified in vitro system. We found that there is almost a complete correlation between resistance to rifampin (Rif super(r)) and reduced rifampin binding to 12 RNAPs purified from different rpoB Rif super(r) mutants and a complete cross-resistance among the different rifamycin derivatives. Most Rif super(r) RNAPs were sensitive to streptolydigin, although some exhibited weak resistance to this antibiotic. However, 5 out of the 12 Rif super(r) RNAPs were partially resistant to sorangicin A, and one was completely cross-resistant to sorangicin A, indicating that the binding site(s) for these two antibiotics overlaps. Both rifampin and sorangicin A inhibited the transition step between transcription initiation and elongation; however, longer abortive initiation products were produced in the presence of the latter, indicating that the binding site for sorangicin A is within the rifampin-binding site. Competition experiments of different antibiotics with super(3)H-labeled rifampin for binding to wild-type RNAP further confirmed that the binding sites for rifampin, rifabutin, rifapentine, and sorangicin A are shared, whereas the binding sites for rifampin and streptolydigin are distinct. Because Rif super(r) mutations are highly conserved in eubacteria, our results indicate that this set of Rif super(r) mutant RNAPs can be used to screen for new antibiotics that will inhibit the growth of Rif super(r) pathogenic bacteria. JF - Journal of Bacteriology AU - Xu, Ming AU - Zhou, Yan Ning AU - Goldstein, Beth P AU - Jin, Ding Jun AD - Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute-Frederick, National Institutes of Health, Frederick. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland. Lepetit Research Center, Gerenzano, Varese, Italy Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 2783 EP - 2792 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 8 SN - 0021-9193, 0021-9193 KW - rifapentine KW - sorangicin A KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - Rifampin KW - DNA-directed RNA polymerase KW - Rifabutin KW - Escherichia coli KW - Cross-resistance KW - Rifamycins KW - Competition KW - Mutation KW - Antibiotic resistance KW - Transcription initiation KW - rpoB gene KW - N 14090:RNA: Ribozymes, Ribonucleoproteins, RNA-binding protein KW - J 02795:Antibiotic resistance UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17872740?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Cross-Resistance+of+Escherichia+coli+RNA+Polymerases+Conferring+Rifampin+Resistance+to+Different+Antibiotics&rft.au=Xu%2C+Ming%3BZhou%2C+Yan+Ning%3BGoldstein%2C+Beth+P%3BJin%2C+Ding+Jun&rft.aulast=Xu&rft.aufirst=Ming&rft.date=2005-04-01&rft.volume=187&rft.issue=8&rft.spage=2783&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Rifampin; DNA-directed RNA polymerase; Rifabutin; Mutation; Competition; Rifamycins; Cross-resistance; Antibiotic resistance; rpoB gene; Transcription initiation; Escherichia coli ER - TY - JOUR T1 - Infrared spectroscopic imaging for histopathologic recognition AN - 17865475; 6251079 AB - The process of histopathology, comprising tissue staining and morphological pattern recognition, has remained largely unchanged for over 140 years. Although it is integral to clinical and research activities, histopathologic recognition remains a time-consuming, subjective process to which only limited statistical confidence can be assigned because of inherent operator variability. Although immunohistochemical approaches allow limited molecular detection, significant challenges remain in using them for quantitative, automated pathology. Vibrational spectroscopic approaches, by contrast, directly provide nonperturbing molecular descriptors, but a practical spectroscopic protocol for histopathology is lacking. Here we couple high-throughput Fourier transform infrared (FTIR) spectroscopic imaging of tissue microarrays with statistical pattern recognition of spectra indicative of endogenous molecular composition and demonstrate histopathologic characterization of prostatic tissue. This automated histologic segmentation is applied to routine archival tissue samples, incorporates well-defined tests of statistical significance and eliminates any requirement for dyes or molecular probes. Finally, we differentiate benign from malignant prostatic epithelium by spectroscopic analyses. JF - Nature Biotechnology AU - Fernandez, Daniel C AU - Bhargava, Rohit AU - Hewitt, Stephen M AU - Levin, Ira W AD - Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892- 0520, USA., iwl@helix.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 469 EP - 474 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 23 IS - 4 SN - 1087-0156, 1087-0156 KW - histopathology KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Bioengineering Abstracts KW - Statistics KW - IR spectroscopy KW - Statistical analysis KW - imaging KW - Pattern recognition KW - Dyes KW - Epithelium KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews KW - W3 33055:Genetic engineering (general) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17865475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Surprising+Dependence+on+Postsegregational+Killing+of+Host+Cells+for+Maintenance+of+the+Large+Virulence+Plasmid+of+Shigella+flexneri&rft.au=Sayeed%2C+Sameera%3BBrendler%2C+Therese%3BDavis%2C+Michael%3BReaves%2C+Lucretia%3BAustin%2C+Stuart&rft.aulast=Sayeed&rft.aufirst=Sameera&rft.date=2005-04-01&rft.volume=187&rft.issue=8&rft.spage=2768&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Statistics; imaging; Pattern recognition; Epithelium; Dyes; Statistical analysis; IR spectroscopy DO - http://dx.doi.org/10.1038/nbt1080 ER - TY - JOUR T1 - Polygenic Effects and Cigarette Smoking Account for a Portion of the Familial Aggregation of Nuclear Sclerosis AN - 17846192; 6244013 AB - Cataract is the most common cause of blindness worldwide. Nuclear cataract, an advanced stage of nuclear sclerosis, is the most common type of age-related cataract. The authors assessed data from 2,089 persons within 620 extended pedigrees who participated in the 1988-1990 Beaver Dam Eye Study in Wisconsin to determine whether the observed familial aggregation of nuclear sclerosis could be explained by inheritance of a major gene. Familial correlations were examined and segregation analyses were performed on nuclear sclerosis measurements adjusted for age, sex, and pack-years of cigarette smoking. There was modest correlation among close family members after adjustment for age, sex, and pack-years of cigarette smoking: 0.084 between parents and offspring, and 0.198 between sibling pairs. Although results do not support involvement of a single major locus in the etiology of nuclear sclerosis, models that allowed for familial correlation, attributable in part to polygenic effects, did provide a better fit to the observed data than models without a polygenic effect. This finding suggests that several genes of modest effect may influence development of nuclear lens opacity, possibly in conjunction with environmental factors. Cigarette smoking was an important covariate in these analyses. Overall, results highlight the complex etiology of nuclear sclerosis. JF - American Journal of Epidemiology AU - Klein, Alison P AU - Duggal, Priya AU - Lee, Kristine E AU - O'Neill, Jennifer A AU - Klein, Ronald AU - Bailey-Wilson, Joan E AU - Klein, Barbara EK AD - Statistical Genetics Section, Inherited Disease Research Branch, National Human Genome Research Institute, Baltimore, MD Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 707 EP - 713 PB - Oxford University Press, Oxford Journals Health, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 161 IS - 8 SN - 0002-9262, 0002-9262 KW - Toxicology Abstracts KW - Etiology KW - Heredity KW - Cataracts KW - Cigarette smoking KW - Eye lens KW - Siblings KW - Progeny KW - Sclerosis KW - Blindness KW - Environmental factors KW - Polygenic inheritance KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17846192?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Epidemiology&rft.atitle=Polygenic+Effects+and+Cigarette+Smoking+Account+for+a+Portion+of+the+Familial+Aggregation+of+Nuclear+Sclerosis&rft.au=Klein%2C+Alison+P%3BDuggal%2C+Priya%3BLee%2C+Kristine+E%3BO%27Neill%2C+Jennifer+A%3BKlein%2C+Ronald%3BBailey-Wilson%2C+Joan+E%3BKlein%2C+Barbara+EK&rft.aulast=Klein&rft.aufirst=Alison&rft.date=2005-04-01&rft.volume=161&rft.issue=8&rft.spage=707&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Sclerosis; Cigarette smoking; Cataracts; Polygenic inheritance; Etiology; Heredity; Progeny; Siblings; Blindness; Environmental factors; Eye lens ER - TY - JOUR T1 - Chlamydial Infection Induces Pathobiotype-Specific Protein Tyrosine Phosphorylation in Epithelial Cells AN - 17827903; 6188724 AB - Members of the genus Chlamydia are strict obligate intracellular pathogens that exhibit marked differences in host range and tissue tropism despite sharing a remarkable level of genomic synteny. These pathobiotype differences among chlamydiae are also mirrored in their early interactions with cultured mammalian host cells. Chlamydial attachment and entry is known to trigger protein tyrosine phosphorylation. In this study, we examined the kinetics and pattern of protein tyrosine phosphorylation induced by infection with a comprehensive collection of chlamydial strains exhibiting diversity in host, tissue, and disease tropisms. We report new findings showing that protein tyrosine phosphorylation patterns induced by infection directly correlate with the pathobiotype of the infecting organism. Patterns of protein tyrosine phosphorylation were induced following early infection that unambiguously categorized chlamydial pathobiotypes into four distinct groups: (i) Chlamydia trachomatis trachoma biovars (serovars A to H), (ii) C. trachomatis lymphogranuloma venereum biovars (serovars L1 to L3), (iii) C. muridarum, and (iv) C. pneumoniae and C. caviae. Notably, chlamydia- infected murine and human epithelial cells exhibited the same protein tyrosine phosphorylation patterns; this is indirect evidence suggesting that the phosphorylated protein(s) is of chlamydial origin. If our hypothesis is correct, these heretofore-uncharacterized proteins may represent a novel class of bacterial molecules that influence pathogen-host range or tissue tropism. JF - Infection and Immunity AU - Virok, Dezso P AU - Nelson, David E AU - Whitmire, William M AU - Crane, Deborah D AU - Goheen, Morgan M AU - Caldwell, Harlan D AD - Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1939 EP - 1946 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 4 SN - 0019-9567, 0019-9567 KW - Microbiology Abstracts B: Bacteriology KW - Synteny KW - Epithelial cells KW - Host range KW - Lymphogranuloma venereum KW - Tropism KW - Tyrosine KW - Chlamydia trachomatis KW - Pathogens KW - Trachoma KW - Phosphorylation KW - Kinetics KW - genomics KW - J 02849:Sexually-transmitted diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17827903?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Chlamydial+Infection+Induces+Pathobiotype-Specific+Protein+Tyrosine+Phosphorylation+in+Epithelial+Cells&rft.au=Virok%2C+Dezso+P%3BNelson%2C+David+E%3BWhitmire%2C+William+M%3BCrane%2C+Deborah+D%3BGoheen%2C+Morgan+M%3BCaldwell%2C+Harlan+D&rft.aulast=Virok&rft.aufirst=Dezso&rft.date=2005-04-01&rft.volume=73&rft.issue=4&rft.spage=1939&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Chlamydia trachomatis; Phosphorylation; Tyrosine; Tropism; Epithelial cells; Host range; Pathogens; Lymphogranuloma venereum; genomics; Kinetics; Synteny; Trachoma ER - TY - JOUR T1 - Uterine Cancer after Use of Clomiphene Citrate to Induce Ovulation AN - 17827847; 6187364 AB - Clomiphene citrate, a selective estrogen receptor modulator, increases estradiol levels and consequently may increase risk of cancer of the uterine corpus. The authors conducted a retrospective cohort study of 8,431 US women (145,876 woman-years) evaluated for infertility during 1965-1988. Through 1999, 39 uterine cancers were ascertained by questionnaire or cancer and death registries. Poisson regression estimated adjusted rate ratios. Study results suggest that clomiphene may increase uterine cancer risk (rate ratio (RR) = 1.79, 95% confidence interval (CI): 0.9, 3.4) and present evidence of a dose response (p sub(trend) = 0.07) and latency effect (p sub(trend) = 0.04). Uterine cancer risk increased with clomiphene dose (RR = 1.93, 95% CI: 0.9, 4.0 for >900 mg), menstrual cycles of use (RR = 2.16, 95% CI: 0.9, 5.2 for =>6 cycles), and time elapsed since initial use (RR = 2.50, 95% CI: 0.9, 7.2 for women followed for =>20 years). Risk was more strongly associated with clomiphene among nulligravid (RR = 3.49, 95% CI: 1.3, 9.3) and obese (RR = 6.02, 95% CI: 1.2, 30.0) women, with risk substantially elevated among women who were both obese and nulligravid (RR = 12.52, 95% CI: 1.5, 108.0). Clomiphene may increase uterine cancer risk, with higher doses leading to higher risk. Long-term follow- up of infertility cohorts is necessary to clarify the association between clomiphene use and uterine cancer. JF - American Journal of Epidemiology AU - Althuis, Michelle D AU - Moghissi, Kamran S AU - Westhoff, Carolyn L AU - Scoccia, Bert AU - Lamb, Emmet J AU - Lubin, Jay H AU - Brinton, Louise A AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 607 EP - 615 PB - Oxford University Press, Oxford Journals Health, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 161 IS - 7 SN - 0002-9262, 0002-9262 KW - clomiphene citrate KW - Clomiphene citrate KW - Risk Abstracts; Toxicology Abstracts; Health & Safety Science Abstracts KW - Infertility KW - Fertility KW - selective estrogen receptor modulators KW - Ovulation KW - Regression analysis KW - Mortality KW - Estradiol KW - Cancer KW - Uterine cancer KW - Side effects KW - Citric acid KW - estrogens KW - R2 23060:Medical and environmental health KW - H 4000:Food and Drugs KW - X 24113:Side effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17827847?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Epidemiology&rft.atitle=Uterine+Cancer+after+Use+of+Clomiphene+Citrate+to+Induce+Ovulation&rft.au=Althuis%2C+Michelle+D%3BMoghissi%2C+Kamran+S%3BWesthoff%2C+Carolyn+L%3BScoccia%2C+Bert%3BLamb%2C+Emmet+J%3BLubin%2C+Jay+H%3BBrinton%2C+Louise+A&rft.aulast=Althuis&rft.aufirst=Michelle&rft.date=2005-04-01&rft.volume=161&rft.issue=7&rft.spage=607&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; Mortality; estrogens; Side effects; Fertility; Uterine cancer; Infertility; Citric acid; Regression analysis; Estradiol; selective estrogen receptor modulators; Ovulation ER - TY - JOUR T1 - Early Role of CD4 super(+) Th1 Cells and Antibodies in HER-2 Adenovirus Vaccine Protection against Autochthonous Mammary Carcinomas AN - 17815458; 6190561 AB - HER-2 is an oncogenic tumor-associated Ag that is overexpressed in several human tumors including breast and ovarian cancer. The efficacy and mechanism of a HER-2-expressing recombinant adenoviral vaccine to protect against tumorigenesis was examined using HER-2 transgenic (BALB-neuT) mice, which develop spontaneous breast tumors in all 10 mammary glands, and also using a transplantable mouse tumor model. Vaccination beginning at 6-8 wk of age (through 19 wk of age) prevented development of spontaneous mammary tumors even after 50 wk, whereas the animals in the control groups had tumors in all mammary glands by 25 wk. Such long-term protection after the last boost has not been achieved previously in this transgenic mouse in which the oncogene is continuously spawning tumorigenesis. Using beta sub(2)-microglobulin-knockout, IFN- gamma -knockout, and B cell-deficient mice, CD4 super(+) and CD8 super(+) cell depletion, and Ab transfer studies, we show that induction of anti-HER-2/neu Abs are both necessary and sufficient for protection, and the IgG2a isotype is most effective. In contrast, CD8 super(+) T cells are not necessary at all, and CD4 super(+) T cells are necessary for only 36-48 h after immunization to provide help for B cells but not as effector cells. Equal protection in immunized mice deficient in Fc gamma RI/III excluded an FcR-mediated mechanism. Anti-HER-2 serum not only inhibited growth of mammary tumor cell lines expressing HER-2 in vitro but also protected mice from tumors in vivo, suggesting a direct action of Ab on the tumor cells. Such a vaccine may provide Ab-mediated protection against HER-2- expressing breast cancers in humans. JF - Journal of Immunology AU - Park, Jong Myun AU - Terabe, Masaki AU - Sakai, Yoshio AU - Munasinghe, Jeeva AU - Forni, Guido AU - Morris, John C AU - Berzofsky, Jay A AD - Vaccine Branch and Cancer Gene Therapy Section, Metabolism Branch, Center for Cancer Research, National Cancer Institute, and Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, Bethesda, MD Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 4228 EP - 4236 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 7 SN - 0022-1767, 0022-1767 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Tumor cells KW - CD4 antigen KW - HER2 protein KW - Oncogenes KW - Lymphocytes T KW - Lymphocytes B KW - Mammary gland KW - Tumorigenesis KW - Adenovirus KW - CD8 antigen KW - Transgenic mice KW - Antibodies KW - Breast cancer KW - F 06818:Cancer immunotherapy KW - W3 33350:Cancer vaccines KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17815458?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Early+Role+of+CD4+super%28%2B%29+Th1+Cells+and+Antibodies+in+HER-2+Adenovirus+Vaccine+Protection+against+Autochthonous+Mammary+Carcinomas&rft.au=Park%2C+Jong+Myun%3BTerabe%2C+Masaki%3BSakai%2C+Yoshio%3BMunasinghe%2C+Jeeva%3BForni%2C+Guido%3BMorris%2C+John+C%3BBerzofsky%2C+Jay+A&rft.aulast=Park&rft.aufirst=Jong&rft.date=2005-04-01&rft.volume=174&rft.issue=7&rft.spage=4228&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adenovirus; Mammary gland; HER2 protein; CD4 antigen; Lymphocytes T; Antibodies; CD8 antigen; Tumorigenesis; Lymphocytes B; Breast cancer; Transgenic mice; Tumor cells; Oncogenes ER - TY - JOUR T1 - Maintenance of Pulmonary Th1 Effector Function in Chronic Tuberculosis Requires Persistent IL-12 Production AN - 17770847; 6190555 AB - The mechanisms that prevent reactivation of latent Mycobacterium tuberculosis infection in asymptomatic individuals are poorly understood. Although IL-12 is critical for the induction of IFN- gamma -dependent host control of M. tuberculosis, the requirement for the cytokine in the maintenance of host resistance and pulmonary Th1 effector function has not yet been formally examined. In this study, we reconstituted IL-12p40-deficient mice with IL-12 during the first 4 wk of infection and then assessed the effects of cytokine withdrawal. Although IL-12 administration initially resulted in restricted mycobacterial growth and prolonged survival, the reconstituted animals eventually succumbed to infection. This breakdown in bacterial control was accompanied by a marked reduction in the numbers of IFN- gamma -producing CD4 super(+) T cells in lungs. Moreover, whereas CD4 super(+) T cells isolated from chronically infected wild-type mice expanded and transferred long-term protection to M. tuberculosis-challenged RAG super(-/-) mice, they failed to do so in IL-12p40- deficient RAG super(-/-) recipients and were clearly reduced in frequency within pulmonary granulomas in the latter animals. These studies establish that continuous IL-12 production is necessary for maintenance of the pulmonary Th1 cells required for host control of persistent M. tuberculosis infection and suggest that breakdown of this mechanism could be a contributing factor in reactivated disease. JF - Journal of Immunology AU - Feng, Carl G AU - Jankovic, Dragana AU - Kullberg, Marika AU - Cheever, Allen AU - Scanga, Charles A AU - Hieny, Sara AU - Caspar, Patricia AU - Yap, George S AU - Sher, Alan AD - Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 4185 EP - 4192 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 7 SN - 0022-1767, 0022-1767 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - g-Interferon KW - Helper cells KW - Granuloma KW - Infection KW - Interleukin 12 KW - CD4 antigen KW - ^g-Interferon KW - Lung KW - Lymphocytes T KW - Tuberculosis KW - Mycobacterium tuberculosis KW - F 06801:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17770847?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Maintenance+of+Pulmonary+Th1+Effector+Function+in+Chronic+Tuberculosis+Requires+Persistent+IL-12+Production&rft.au=Feng%2C+Carl+G%3BJankovic%2C+Dragana%3BKullberg%2C+Marika%3BCheever%2C+Allen%3BScanga%2C+Charles+A%3BHieny%2C+Sara%3BCaspar%2C+Patricia%3BYap%2C+George+S%3BSher%2C+Alan&rft.aulast=Feng&rft.aufirst=Carl&rft.date=2005-04-01&rft.volume=174&rft.issue=7&rft.spage=4185&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; Interleukin 12; Lymphocytes T; Infection; Lung; Tuberculosis; Helper cells; g-Interferon; CD4 antigen; Granuloma; ^g-Interferon ER - TY - JOUR T1 - Use of housekeeping gene sequencing for species identification of viridans streptococci AN - 17654360; 6445287 AB - Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis-sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified. JF - Diagnostic Microbiology and Infectious Disease AU - Kiratisin, P AU - Li, L AU - Murray, PR AU - Fischer, SH AD - Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, sfischer@cc.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 297 EP - 301 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 51 IS - 4 SN - 0732-8893, 0732-8893 KW - streptococci KW - Microbiology Abstracts B: Bacteriology KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17654360?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Diagnostic+Microbiology+and+Infectious+Disease&rft.atitle=Use+of+housekeeping+gene+sequencing+for+species+identification+of+viridans+streptococci&rft.au=Kiratisin%2C+P%3BLi%2C+L%3BMurray%2C+PR%3BFischer%2C+SH&rft.aulast=Kiratisin&rft.aufirst=P&rft.date=2005-04-01&rft.volume=51&rft.issue=4&rft.spage=297&rft.isbn=&rft.btitle=&rft.title=Diagnostic+Microbiology+and+Infectious+Disease&rft.issn=07328893&rft_id=info:doi/10.1016%2Fj.diagmicrobio.2004.12.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1016/j.diagmicrobio.2004.12.001 ER - TY - JOUR T1 - MC1R and the response of melanocytes to ultraviolet radiation AN - 17642593; 6389291 AB - The constitutive color of our skin plays a dramatic role in our photoprotection from solar ultraviolet radiation (UVR) that reaches the Earth and in minimizing DNA damage that gives rise to skin cancer. More than 120 genes have been identified and shown to regulate pigmentation, one of the key genes being melanocortin 1 receptor (MC1R) that encodes the melanocortin 1 receptor (MC1R), a seven-transmembrane G protein-coupled receptor expressed on the surface of melanocytes. Modulation of MC1R function regulates melanin synthesis by melanocytes qualitatively and quantitatively. The MC1R is regulated by the physiological agonists alpha -melanocyte-stimulating hormone ( alpha MSH) and adrenocorticotropic hormone (ACTH), and antagonist agouti signaling protein (ASP). Activation of the MC1R by binding of an agonist stimulates the synthesis of eumelanin primarily via activation of adenylate cyclase. The significance of cutaneous pigmentation lies in the photoprotective effect of melanin, particularly eumelanin, against sun-induced carcinogenesis. Epidermal melanocytes and keratinocytes respond to UVR by increasing their expression of alpha MSH and ACTH, which up-regulate the expression of MC1R, and consequently enhance the response of melanocytes to melanocortins. Constitutive skin pigmentation dramatically affects the incidence of skin cancer. The pigmentary phenotype characterized by red hair, fair complexion, inability to tan and tendency to freckle is an independent risk factor for all skin cancers, including melanoma. The MC1R gene is highly polymorphic in human populations, and allelic variation at this locus accounts, to a large extent, for the variation in pigmentary phenotypes and skin phototypes (SPT) in humans. Several allelic variants of the MC1R gene are associated with the red hair and fair skin (RHC) phenotype, and carrying one of these variants is thought to diminish the ability of the epidermis to respond to DNA damage elicited by UVR. The MC1R gene is considered a melanoma susceptibility gene, and its significance in determining the risk for skin cancer is of tremendous interest. JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis AU - Rouzaud, F AU - Kadekaro, AL AU - Abdel-Malek, Z A AU - Hearing, V J AD - National Cancer Institute, National Institutes of Health, Building 37, Room 2132, Bethesda, MD 20892, USA, hearingv@nih.gov Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 133 EP - 152 PB - Elsevier B.V. VL - 571 IS - 1-2 SN - 0027-5107, 0027-5107 KW - Genetics Abstracts; Toxicology Abstracts KW - X 24210:Radiation & radioactive materials KW - G 07470:Cytogenetics & general UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17642593?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.atitle=MC1R+and+the+response+of+melanocytes+to+ultraviolet+radiation&rft.au=Rouzaud%2C+F%3BKadekaro%2C+AL%3BAbdel-Malek%2C+Z+A%3BHearing%2C+V+J&rft.aulast=Rouzaud&rft.aufirst=F&rft.date=2005-04-01&rft.volume=571&rft.issue=1-2&rft.spage=133&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.issn=00275107&rft_id=info:doi/10.1016%2Fj.mrfmmm.2004.09.014 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-10-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1016/j.mrfmmm.2004.09.014 ER - TY - JOUR T1 - Global Transcriptional Programs Reveal a Carbon Source Foraging Strategy by Escherichia coli AN - 17620981; 6274508 AB - By exploring global gene expression of Escherichia coli growing on six different carbon sources, we discovered a striking genome transcription pattern: as carbon substrate quality declines, cells systematically increase the number of genes expressed. Gene induction occurs in a hierarchical manner and includes many factors for uptake and metabolism of better but currently unavailable carbon sources. Concomitantly, cells also increase their motility. Thus, as the growth potential of the environment decreases, cells appear to devote progressively more energy on the mere possibility of improving conditions. This adaptation is not what would be predicated by classic regulatory models alone. We also observe an inverse correlation between gene activation and rRNA synthesis suggesting that reapportioning RNA polymerase (RNAP) contributes to the expanded genome activation. Significant differences in RNAP distribution in vivo, monitored using an RNAP-green fluorescent protein fusion, from energy-rich and energy-poor carbon source cultures support this hypothesis. Together, these findings represent the integration of both substrate-specific and global regulatory systems, and may be a bacterial approximation to metazoan risk-prone foraging behavior. JF - Journal of Biological Chemistry AU - Liu, Mingzhu AU - Durfee, Tim AU - Cabrera, Julio E AU - Zhao, Kai AU - Jin, Ding J AU - Blattner, Frederick R AD - Department of Genetics and McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706 and the Transcription Control Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, NCI at Frederick, National Institutes of Health, Frederick, Maryland 21702 Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 15921 EP - 15927 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 16 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - J 02726:RNA and ribosomes KW - N 14045:Transcriptional regulation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17620981?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Global+Transcriptional+Programs+Reveal+a+Carbon+Source+Foraging+Strategy+by+Escherichia+coli&rft.au=Liu%2C+Mingzhu%3BDurfee%2C+Tim%3BCabrera%2C+Julio+E%3BZhao%2C+Kai%3BJin%2C+Ding+J%3BBlattner%2C+Frederick+R&rft.aulast=Liu&rft.aufirst=Mingzhu&rft.date=2005-04-01&rft.volume=280&rft.issue=16&rft.spage=15921&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Systematic variation normalization in microarray data to get gene expression comparison unbiased AN - 17574477; 6386147 AB - Normalization removes or minimizes the biases of systematic variation that exists in experimental data sets. This study presents a systematic variation normalization (SVN) procedure for removing systematic variation in two channel microarray gene expression data. Based on an analysis of how systematic variation contributes to variability in microarray data sets, our normalization procedure includes background subtraction determined from the distribution of pixel intensity values from each data acquisition channel and log conversion, linear or non-linear regression, restoration or transformation, and multiarray normalization. In the case when a non-linear regression is required, an empirical polynomial approximation approach is used. Either the high terminated points or their averaged values in the distributions of the pixel intensity values observed in control channels may be used for rescaling multiarray datasets. These pre-processing steps remove systematic variation in the data attributable to variability in microarray slides, assay-batches, the array process, or experimenters. Biologically meaningful. JF - Journal of Bioinformatics and Computational Biology AU - Chou, J W AU - Paules, R S AU - Bushel, PR AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709, USA, chou@nichs.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 225 EP - 241 VL - 3 IS - 2 SN - 0219-7200, 0219-7200 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Databases KW - Mathematical models KW - Data processing KW - Bioinformatics KW - DNA microarrays KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17574477?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bioinformatics+and+Computational+Biology&rft.atitle=Systematic+variation+normalization+in+microarray+data+to+get+gene+expression+comparison+unbiased&rft.au=Chou%2C+J+W%3BPaules%2C+R+S%3BBushel%2C+PR&rft.aulast=Chou&rft.aufirst=J&rft.date=2005-04-01&rft.volume=3&rft.issue=2&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bioinformatics+and+Computational+Biology&rft.issn=02197200&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Data processing; DNA microarrays; Gene expression; Databases; Mathematical models; Bioinformatics ER - TY - JOUR T1 - Effects of Electron-Beam Irradiation on Whole Genome Amplification AN - 17540509; 6269758 AB - Electron-beam (E-beam) irradiation, currently being used to sterilize mail addressed to selected ZIP codes in the United States, has significant negative effects on the genomic integrity of DNA extracted from buccal-cell washes. We investigated the yield, composition, and genotyping performance of whole genome amplified DNA (wgaDNA) derived from 24 matched samples of E-beam-irradiated and nonirradiated genomic DNA (gDNA) as a model for the effects of degraded gDNA on the performance of whole genome amplification. gDNA was amplified using the Multiple Displacement Amplification method. Three methods of DNA quantification analysis were used to estimate the yield and composition of wgaDNA, and 65 short tandem repeat and single nucleotide polymorphism genotyping assays were used to evaluate the genotyping performance of irradiated and nonirradiated gDNA and wgaDNA. Compared with wgaDNA derived from nonirradiated gDNA, wgaDNA derived from irradiated gDNA exhibited a significantly reduced yield of wgaDNA and significantly reduced short tandem repeat and single nucleotide polymorphism genotyping completion and concordance rates (P < 0.0001). Increasing the amount of irradiated gDNA input into whole genome amplification improved genotyping performance of wgaDNA but not to the level of wgaDNA derived from nonirradiated gDNA. Multiple Displacement Amplification wgaDNA derived from E-beam-irradiated gDNA is not suitable for genotyping analysis. JF - Cancer Epidemiology, Biomarkers & Prevention AU - Bergen, Andrew W AU - Qi, Ying AU - Haque, Kashif A AU - Welch, Robert A AU - Garcia-Closas, Montserrat AU - Chanock, Stephen J AU - Vaught, Jim AU - Castle, Philip E AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1016 EP - 1019 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 4 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Genomes KW - Short tandem repeats KW - Radiation KW - Single-nucleotide polymorphism KW - Genotyping KW - DNA KW - genomics KW - biomarkers KW - Models KW - X 24210:Radiation & radioactive materials KW - N 14020:DNA/RNA genomics sequence UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17540509?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Effects+of+Electron-Beam+Irradiation+on+Whole+Genome+Amplification&rft.au=Bergen%2C+Andrew+W%3BQi%2C+Ying%3BHaque%2C+Kashif+A%3BWelch%2C+Robert+A%3BGarcia-Closas%2C+Montserrat%3BChanock%2C+Stephen+J%3BVaught%2C+Jim%3BCastle%2C+Philip+E&rft.aulast=Bergen&rft.aufirst=Andrew&rft.date=2005-04-01&rft.volume=14&rft.issue=4&rft.spage=1016&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Genomes; Short tandem repeats; Radiation; Single-nucleotide polymorphism; Genotyping; DNA; genomics; biomarkers; Models ER - TY - JOUR T1 - A structure-based method for protein sequence alignment AN - 17540433; 6244425 AB - MOTIVATION: With the continuing rapid growth of protein sequence data, protein sequence comparison methods have become the most widely used tools of bioinformatics. Among these methods are those that use position-specific scoring matrices (PSSMs) to describe protein families. PSSMs can capture information about conserved patterns within families, which can be used to increase the sensitivity of searches for related sequences. Certain types of structural information, however, are not generally captured by PSSM search methods. Here we introduce a program, Structure-based ALignment TOol (SALTO), that aligns protein query sequences to PSSMs using rules for placing and scoring gaps that are consistent with the conserved regions of domain alignments from NCBI's Conserved Domain Database. RESULTS: In most cases, the alignment scores obtained using the local alignment version follow an extreme value distribution. SALTO's performance in finding related sequences and producing accurate alignments is similar to or better than that of IMPALA; one advantage of SALTO is that it imposes an explicit gapping model on each protein family. AVAILABILITY: A stand-alone version of the program that can generate global or local alignments is available by ftp distribution (ftp://ftp.ncbi.nih.gov/pub/SALTO/), and has been incorporated to Cn3D structure/alignment viewer. JF - Bioinformatics AU - Kann, Maricel G AU - Thiessen, Paul A AU - Panchenko, Anna R AU - Schaeffer, Alejandro A AU - Altschul, Stephen F AU - Bryant, Stephen H AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Department of Health and Human Services Bethesda, MD 20894, USA, bryant@ncbi.nlm.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1451 EP - 1456 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 8 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Protein structure KW - Computer programs KW - Databases KW - protein families KW - Bioinformatics KW - Amino acid sequence KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17540433?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=A+structure-based+method+for+protein+sequence+alignment&rft.au=Kann%2C+Maricel+G%3BThiessen%2C+Paul+A%3BPanchenko%2C+Anna+R%3BSchaeffer%2C+Alejandro+A%3BAltschul%2C+Stephen+F%3BBryant%2C+Stephen+H&rft.aulast=Kann&rft.aufirst=Maricel&rft.date=2005-04-01&rft.volume=21&rft.issue=8&rft.spage=1451&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Amino acid sequence; protein families; Bioinformatics; Databases; Computer programs; Protein structure ER - TY - JOUR T1 - Surprising Dependence on Postsegregational Killing of Host Cells for Maintenance of the Large Virulence Plasmid of Shigella flexneri AN - 17539047; 6245816 AB - Low-copy-number plasmids all encode multiple systems to ensure their propagation, including replication, partition (active segregation), and postsegregational killing (PSK) systems. PSK systems kill those rare cells that lose the plasmid due to replication or segregation errors. PSK systems should not be used as the principle means of maintaining the plasmid. The metabolic cost of killing the many cured cells that would arise from random plasmid segregation is far too high. Here we describe an interesting exception to this rule. Maintenance of the large virulence plasmid of Shigella flexneri is highly dependent on one of its PSK systems, mvp, at 37 degree C, the temperature experienced during pathogenesis. At 37 degree C, the plasmid is very unstable and mvp efficiently kills the resulting cured bacterial cells. This imposes a major growth disadvantage on the virulent bacterial population. The systems that normally ensure accurate plasmid replication and segregation are attenuated or overridden at 37 degree C. At 30 degree C, a temperature encountered by Shigella in the outside environment, the maintenance systems function normally and the plasmid is no longer dependent on mvp. We discuss why the virulent pathogen tolerates this self-destructive method of propagation at the temperature of infection. JF - Journal of Bacteriology AU - Sayeed, Sameera AU - Brendler, Therese AU - Davis, Michael AU - Reaves, Lucretia AU - Austin, Stuart AD - Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute-Frederick, Frederick, Maryland. Department of Biological Sciences, Central Connecticut State University, New Britain, Connecticut Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 2768 EP - 2773 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 8 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - Temperature effects KW - Replication KW - Pathogens KW - Infection KW - Plasmids KW - Virulence KW - Shigella flexneri KW - N 14025:RNA/DNA role in infection & immune response KW - J 02760:Plasmids UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17539047?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Surprising+Dependence+on+Postsegregational+Killing+of+Host+Cells+for+Maintenance+of+the+Large+Virulence+Plasmid+of+Shigella+flexneri&rft.au=Sayeed%2C+Sameera%3BBrendler%2C+Therese%3BDavis%2C+Michael%3BReaves%2C+Lucretia%3BAustin%2C+Stuart&rft.aulast=Sayeed&rft.aufirst=Sameera&rft.date=2005-04-01&rft.volume=187&rft.issue=8&rft.spage=2768&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Shigella flexneri; Plasmids; Temperature effects; Replication; Virulence; Infection; Pathogens ER - TY - JOUR T1 - Boolean relationships among genes responsive to ionizing radiation in the NCI 60 ACDS AN - 17536496; 6244437 AB - MOTIVATION: An early use of gene-expression data coming from microarrays was to discover non-linear multivariate intergene relationships. Pursuing this direction, the motivation for this paper is 2-fold: (1) to discover and elucidate multivariate logical predictive relations among gene expressions in a dataset arising from radiation studies using the NCI 60 Anti-Cancer Drug Screen (ACDS) cell lines; and (2) to demonstrate how these logical relations based on coarse quantization reflect corresponding relations in the continuous data. RESULTS: Using the coefficient of determination, a large number of logical relationships have been discovered among genes in the NCI 60 ACDS cell lines. Moreover, these relationships can be seen directly in the original continuous data, and many are robust relative to the thresholds used to obtain the logical data from the continuous data. A key observation is that a number of intergene relationships appear to be considerably stronger when p53 is functional as compared to when it is not, which is consistent with earlier findings in the literature. AVAILABILITY: The appendix is available at http://gsp.tamu.edu/Publications/supplement.htm JF - Bioinformatics AU - Pal, Ranadip AU - Datta, Aniruddha AU - Fornace, Albert JJr AU - Bittner, Michael L AU - Dougherty, Edward R AD - Department of Electrical Engineering, Texas A&M University College Station, TX 77843, USA. National Cancer Institute NIH Bethesda, MD 20892, USA. Translational Genomics Research Institute 400 North Fifth Street, Suite 1600, Phoenix, AZ 85004, USA. University of Texas M.D. Anderson Cancer Center Houston, TX 77030, USA, edward@ee.tamu.edu Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 1542 EP - 1549 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 8 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Ionizing radiation KW - Appendix KW - Bioinformatics KW - DNA microarrays KW - p53 protein KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17536496?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Boolean+relationships+among+genes+responsive+to+ionizing+radiation+in+the+NCI+60+ACDS&rft.au=Pal%2C+Ranadip%3BDatta%2C+Aniruddha%3BFornace%2C+Albert+JJr%3BBittner%2C+Michael+L%3BDougherty%2C+Edward+R&rft.aulast=Pal&rft.aufirst=Ranadip&rft.date=2005-04-01&rft.volume=21&rft.issue=8&rft.spage=1542&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Gene expression; DNA microarrays; Ionizing radiation; Appendix; p53 protein; Bioinformatics ER - TY - JOUR T1 - Dynamic Fluorescent Imaging of Human Immunodeficiency Virus Type 1 Gag in Live Cells by Biarsenical Labeling AN - 17529347; 6185782 AB - Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both Pr55 super(Gag) expression and full-length proviral constructs. Membrane-permeable biarsenical compounds FlAsH and ReAsH covalently bond to this tetracysteine sequence and specifically fluoresce, effectively labeling Gag in the cell. Biarsenical labeling readily and specifically detected a tetracysteine-tagged HIV-1 Gag protein (Gag-TC) in HeLa, Mel JuSo, and Jurkat T cells by deconvolution fluorescence microscopy. Gag-TC was localized primarily at or near the plasma membrane in all cell types examined. Fluorescent two-color analysis of Gag-TC in HeLa cells revealed that nascent Gag was present mostly at the plasma membrane in distinct regions. Intracellular imaging of a Gag-TC myristylation mutant observed a diffuse signal throughout the cell, consistent with the role of myristylation in Gag localization to the plasma membrane. In contrast, mutation of the L-domain core sequence did not appreciably alter the localization of Gag, suggesting that the PTAP L domain functions at the site of budding rather than as a targeting signal. Taken together, our results show that Gag concentrates in specific plasma membrane areas rapidly after translation and demonstrate the utility of biarsenical labeling for visualizing the dynamic localization of Gag. JF - Journal of Virology AU - Rudner, Lynnie AU - Nydegger, Sascha AU - Coren, Lori V AU - Nagashima, Kunio AU - Thali, Markus AU - Ott, David E AD - Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont. Basic Research. Research Technology Programs, SAIC- Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 4055 EP - 4065 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 7 SN - 0022-538X, 0022-538X KW - HIV-1 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts; Virology & AIDS Abstracts KW - Translation KW - Fluorescence KW - Plasma membranes KW - Microscopy KW - Human immunodeficiency virus 1 KW - Lymphocytes T KW - Mutation KW - imaging KW - Structural proteins KW - Gag protein KW - Budding KW - V 22002:AIDS: Molecular and in vitro aspects KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17529347?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Dynamic+Fluorescent+Imaging+of+Human+Immunodeficiency+Virus+Type+1+Gag+in+Live+Cells+by+Biarsenical+Labeling&rft.au=Rudner%2C+Lynnie%3BNydegger%2C+Sascha%3BCoren%2C+Lori+V%3BNagashima%2C+Kunio%3BThali%2C+Markus%3BOtt%2C+David+E&rft.aulast=Rudner&rft.aufirst=Lynnie&rft.date=2005-04-01&rft.volume=79&rft.issue=7&rft.spage=4055&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Human immunodeficiency virus 1; Plasma membranes; imaging; Translation; Microscopy; Gag protein; Fluorescence; Budding; Lymphocytes T; Structural proteins; Mutation ER - TY - JOUR T1 - Transfer of a TCR Gene Derived from a Patient with a Marked Antitumor Response Conveys Highly Active T-Cell Effector Functions AN - 17523832; 6238009 AB - The genes for the alpha and beta chains of a highly reactive anti-MART-1 T-cell receptor were isolated from T-lymphocytes that mediated in vivo regression of tumor in a patient with metastatic melanoma. These genes were cloned and inserted into MSCV-based retroviral vectors. After transduction, greater than 50% gene transfer efficiency was demonstrated in primary T-lymphocytes stimulated by an anti-CD3 antibody. The specificity and biologic activity of TCR gene-transduced T-cells was determined by cytokine production after coculture of T-cells with stimulator cells pulsed with MART-1 peptide. The production of interferon- gamma and granulocyte macrophage-colony stimulating factor (GM-CSF) was comparable to highly active MART-1 specific peripheral blood lymphocytes (PBL) in the amount of cytokine produced and transduced cells recognized peptide pulsed cells at dilutions similar to cytotoxic T lymphocyte (CTL) clones. Human leukocyte antigen (HLA) class I restricted recognition was demonstrated by mobilization of degranulation marker CD107a, by cell lysis, by cytokine production, and by proliferation in the presence of HLA-A2-positive but not HLA-A2-negative melanoma cell lines. Similar data was obtained when tumor-infiltrating lymphocytes (TIL) were transduced with the TCR genes, converting previously nonreactive cells to tumor reactive cells. TCR-transduced T-cells are thus attractive candidates for evaluation in cell transfer therapies of patients with cancer. JF - Human Gene Therapy AU - Hughes AU - Yu, YYL AU - Dudley, ME AU - Zheng, Z AU - Robbins, P F AU - Li, Y AU - Wunderlich, J AU - Hawley, R G AU - Moayeri, M AU - Rosenberg, SA AU - Morgan, R A AD - National Cancer Institute Surgery Branch, National Institutes of Health, Building 10 CRC rm 3-5940, 10 Center Drive, Bethesda, MD 20892-1201, USA, rmorgan@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 457 EP - 472 VL - 16 IS - 4 SN - 1043-0342, 1043-0342 KW - Biotechnology and Bioengineering Abstracts; Genetics Abstracts; Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Histocompatibility antigen HLA KW - T-cell receptor KW - Melanoma KW - Metastases KW - Lymphocytes T KW - Cytokines KW - g-Interferon KW - Gene therapy KW - Granulocyte-macrophage colony-stimulating factor KW - Peripheral blood KW - Tumor-infiltrating lymphocytes KW - Cancer KW - Cytotoxicity KW - ^AT-cell receptor KW - Antibodies KW - MART-1 antigen KW - ^g-Interferon KW - W3 33181:Gene therapy vectors KW - G 07443:Gene therapy KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17523832?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+Gene+Therapy&rft.atitle=Transfer+of+a+TCR+Gene+Derived+from+a+Patient+with+a+Marked+Antitumor+Response+Conveys+Highly+Active+T-Cell+Effector+Functions&rft.au=Hughes%3BYu%2C+YYL%3BDudley%2C+ME%3BZheng%2C+Z%3BRobbins%2C+P+F%3BLi%2C+Y%3BWunderlich%2C+J%3BHawley%2C+R+G%3BMoayeri%2C+M%3BRosenberg%2C+SA%3BMorgan%2C+R+A&rft.aulast=Hughes&rft.aufirst=&rft.date=2005-04-01&rft.volume=16&rft.issue=4&rft.spage=457&rft.isbn=&rft.btitle=&rft.title=Human+Gene+Therapy&rft.issn=10430342&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Lymphocytes T; T-cell receptor; Histocompatibility antigen HLA; Cytokines; MART-1 antigen; Granulocyte-macrophage colony-stimulating factor; Melanoma; g-Interferon; Antibodies; Tumor-infiltrating lymphocytes; Cancer; Gene therapy; ^AT-cell receptor; ^g-Interferon; Cytotoxicity; Peripheral blood; Metastases ER - TY - JOUR T1 - A nationwide survey of physician office visits found that inappropriate antibiotic prescriptions were issued for bacterial respiratory tract infections in ambulatory patients AN - 17502669; 6398200 AB - Correlations between probabilities of resistance and the frequencies with which antibiotics were prescribed for treating bacterial respiratory infections were examined in a nationwide ambulatory population. The data of a nationwide probability sample survey of visits to physician offices in the United States in 1999 were used to conduct this study of drug use. A clinical pharmacologist identified antibiotics prescribed during those visits using a large online database. The participating physicians diagnosed the bacterial respiratory infections. An infectious disease expert determined the probabilities of bacterial resistance from a nationwide antibiotic surveillance database. Various bacterial respiratory infections were diagnosed during 6.5% of physician office visits in 1999. One or more antibiotics were prescribed during 51.0% of those visits. The probabilities of resistance to the most frequently prescribed antibiotics varied from 20% to 40% and showed a weak positive correlation with the frequencies of antibiotic prescriptions. A significant number of inappropriate antibiotic prescriptions were issued for infections with a high probability of bacterial resistance to the prescribed antibiotics. JF - Journal of Clinical Epidemiology AU - Huang, Boji AU - Martin, S J AU - Bachmann, KA AU - He, Xuming AU - Reese, J H AU - Wei, Ying AU - Iwuagwu, C AD - Department of Health Evaluation Sciences, Penn State College of Medicine, 600 Centerview Drive, Hershey, PA 17022-0855, USA, HuangBo@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 414 EP - 420 VL - 58 IS - 4 SN - 0895-4356, 0895-4356 KW - Microbiology Abstracts B: Bacteriology KW - Databases KW - Respiratory tract diseases KW - USA KW - Infectious diseases KW - Antibiotic resistance KW - Medical personnel KW - J 02845:Ear, nose and respiratory tract UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17502669?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Epidemiology&rft.atitle=A+nationwide+survey+of+physician+office+visits+found+that+inappropriate+antibiotic+prescriptions+were+issued+for+bacterial+respiratory+tract+infections+in+ambulatory+patients&rft.au=Huang%2C+Boji%3BMartin%2C+S+J%3BBachmann%2C+KA%3BHe%2C+Xuming%3BReese%2C+J+H%3BWei%2C+Ying%3BIwuagwu%2C+C&rft.aulast=Huang&rft.aufirst=Boji&rft.date=2005-04-01&rft.volume=58&rft.issue=4&rft.spage=414&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Epidemiology&rft.issn=08954356&rft_id=info:doi/10.1016%2Fj.jclinepi.2004.09.006 LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Respiratory tract diseases; Databases; Infectious diseases; Medical personnel; Antibiotic resistance; USA DO - http://dx.doi.org/10.1016/j.jclinepi.2004.09.006 ER - TY - JOUR T1 - The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression AN - 17502145; 6397737 AB - Background Recombinant adeno-associated viruses (rAAV) are commonly used in pre-clinical and clinical gene transfer studies. However, the relatively slow kinetics of rAAV transgene expression complicates in vitro and in vivo experiments. Methods 293 and COS-1 cells were transduced with rAAV2-EGFP, rAAV1-EGFP, or rAAV5-EGFP. The rAAV-EGFP expression was analyzed in the presence of Hoechst 33 258 or 33 342 as a function of time and concentration by flow cytometry and fluorescent microscope. Effects of Hoechst on cell cycle populations were determined by flow cytometry. Enhanced green fluorescent protein (EGFP) expression plasmids with or without AAV inverted terminal repeats (ITR) were constructed and gene expression by transient transfection was compared in the presence of Hoechst. Results We found that Hoechst 33 258 and 33 342 increase both the level and the population of EGFP gene expressing cells, transduced by several different serotypes of rAAV-EGFP. The augmentation of rAAV-EGFP expression occurs in different cell types in a concentration-dependent manner. In addition, the Hoechst 33 258 or 33 342 mediated enhancement of rAAV gene expression correlated with an increase of cells in S phase and G2/M phases of the cell cycle. Finally, gene expression from transfected ITR-containing plasmid DNA was also enhanced by Hoechst dyes. Conclusions Our results revealed that two different, although related, DNA-binding drugs, Hoechst 33 258 and 33 342, accelerate the kinetics of rAAV transgene expression. These findings may provide the basis for more sensitive assessment of rAAV biological activity and also extend the applications of rAAV for in vivo gene transfer. JF - Journal of Gene Medicine AU - Li, L AU - Yang, L AU - Kotin, R M AD - Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bldg. 10, Rm. 7D05, 10 Center Dr., Bethesda, MD 20892, USA, kotinr@nhlbi.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 420 EP - 431 VL - 7 IS - 4 SN - 1099-498X, 1099-498X KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Cell cycle KW - Green fluorescent protein KW - Plasmids KW - Adeno-associated virus KW - Flow cytometry KW - Gene expression KW - S phase KW - Transfection KW - Gene transfer KW - W3 33181:Gene therapy vectors KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17502145?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Gene+Medicine&rft.atitle=The+DNA+minor+groove+binding+agents+Hoechst+33258+and+33342+enhance+recombinant+adeno-associated+virus+%28rAAV%29+transgene+expression&rft.au=Li%2C+L%3BYang%2C+L%3BKotin%2C+R+M&rft.aulast=Li&rft.aufirst=L&rft.date=2005-04-01&rft.volume=7&rft.issue=4&rft.spage=420&rft.isbn=&rft.btitle=&rft.title=Journal+of+Gene+Medicine&rft.issn=1099498X&rft_id=info:doi/10.1002%2Fjgm.681 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adeno-associated virus; Gene expression; Flow cytometry; Plasmids; Cell cycle; Gene transfer; Transfection; S phase; Green fluorescent protein DO - http://dx.doi.org/10.1002/jgm.681 ER - TY - JOUR T1 - Active Tolerance Induction and Prevention of Autoimmune Diabetes by Immunogene Therapy Using Recombinant Adenoassociated Virus Expressing Glutamic Acid Decarboxylase 65 Peptide GAD sub(500-585) AN - 17500552; 6267133 AB - Tolerance induction of autoreactive T cells against pancreatic beta cell-specific autoantigens such as glutamic acid decarboxylase 65 (GAD65) and insulin has been attempted as a method to prevent autoimmune diabetes. In this study, we investigate whether adenoassociated virus (AAV) gene delivery of multiple immunodominant epitopes expressing GAD sub(500-585) could induce potent immune tolerance and persistently suppress autoimmune diabetes in NOD mice. A single muscle injection of 7-wk-old female NOD mice with rAAV/GAD sub(500-585) (3 x 10 super(11) IU/mouse) quantitatively reduced pancreatic insulitis and efficiently prevented the development of overt type I diabetes. This prevention was marked by the inactivation of GAD sub(500-585)-responsive T lymphocytes, the enhanced GAD sub(500-585)-specific Th2 response (characterized by increased IL-4, IL-10 production, and decreased IFN- gamma production; especially elevated anti-GAD sub(500-585) IgG1 titer; and relatively unchanged anti-GAD sub(500-585) IgG2b titer), the increased secretion of TGF- beta , and the production of protective regulatory cells. Our studies also revealed that peptides 509-528, 570-585, and 554-546 in the region of GAD sub(500-585) played important roles in rAAV/GAD sub(500-585) immunization-induced immune tolerance. These data indicate that using AAV, a vector with advantage for therapeutic gene delivery, to transfer autoantigen peptide GAD sub(500-585), can induce immunological tolerance through active suppression of effector T cells and prevent type I diabetes in NOD mice. JF - Journal of Immunology AU - Han, Gencheng AU - Li, Yan AU - Wang, Jianan AU - Wang, Renxi AU - Chen, Guojiang AU - Song, Lun AU - Xu, Ruonan AU - Yu, Ming AU - Wu, Xiaobing AU - Qian, Jiahua AU - Shen, Beifen AD - Department of Molecular Immunology, Institute of Basic Medical Sciences, and. National Lab of Molecular Virology and Genetic Engineering, Beijing, People's Republic of China. National Cancer Institute, Vaccine Branch, Bethesda, MD 20889 Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 4516 EP - 4524 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 8 SN - 0022-1767, 0022-1767 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Immunoregulation KW - Pancreas KW - Autoimmune diseases KW - Insulin KW - Adeno-associated virus KW - Lymphocytes T KW - g-Interferon KW - Immunological tolerance KW - Autoantigens KW - Diabetes mellitus KW - ^g-Interferon KW - Gene transfer KW - Immunoglobulin G KW - Glutamate decarboxylase KW - F 06328:Diabetes: Animal models KW - W 30965:Miscellaneous, Reviews KW - W3 33180:Gene based (protocols, clinical trials, and animal models) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17500552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Active+Tolerance+Induction+and+Prevention+of+Autoimmune+Diabetes+by+Immunogene+Therapy+Using+Recombinant+Adenoassociated+Virus+Expressing+Glutamic+Acid+Decarboxylase+65+Peptide+GAD+sub%28500-585%29&rft.au=Han%2C+Gencheng%3BLi%2C+Yan%3BWang%2C+Jianan%3BWang%2C+Renxi%3BChen%2C+Guojiang%3BSong%2C+Lun%3BXu%2C+Ruonan%3BYu%2C+Ming%3BWu%2C+Xiaobing%3BQian%2C+Jiahua%3BShen%2C+Beifen&rft.aulast=Han&rft.aufirst=Gencheng&rft.date=2005-04-01&rft.volume=174&rft.issue=8&rft.spage=4516&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adeno-associated virus; Lymphocytes T; Immunological tolerance; Diabetes mellitus; Autoimmune diseases; Pancreas; Autoantigens; Gene transfer; Glutamate decarboxylase; Immunoglobulin G; g-Interferon; Immunoregulation; Insulin; ^g-Interferon ER - TY - JOUR T1 - The Molecular Chaperone, ClpA, Has a Single High Affinity Peptide Binding Site per Hexamer AN - 17498348; 6266578 AB - Substrate recognition by Clp chaperones is dependent on interactions with motifs composed of specific peptide sequences. We studied the binding of short motif-bearing peptides to ClpA, the chaperone component of the ATP-dependent ClpAP protease of Escherichia coli in the presence of ATP gamma S and Mg super(2+) at pH 7.5. Binding was measured by isothermal titration calorimetry (ITC) using the peptide, AANDENYALAA, which corresponds to the SsrA degradation motif found at the C terminus of abnormal nascent polypeptides in vivo. One SsrA peptide was bound per hexamer of ClpA with an association constant (K sub(A)) of 5 x 10 super(6) M super(-1). Binding was also assayed by changes in fluorescence of an N-terminal dansylated SsrA peptide, which bound with the same stoichiometry of one per ClpA hexamer (K sub(A) similar to 1 x 10 super(7) M super(-1)). Similar results were obtained when ATP was substituted for ATP gamma S at 6 degree C. Two additional peptides, derived from the phage P1 RepA protein and the E. coli HemA protein, which bear different substrate motifs, were competitive inhibitors of SsrA binding and bound to ClpA hexamers with K sub(A)' > 3 x 10 super(7) M super(-1). DNS-SsrA bound with only slightly reduced affinity to deletion mutants of ClpA missing either the N-terminal domain or the C-terminal nucleotide-binding domain, indicating that the binding site for SsrA lies within the N-terminal nucleotide-binding domain. Because only one protein at a time can be unfolded and translocated by ClpA hexamers, restricting the number of peptides initially bound should avoid nonproductive binding of substrates and aggregation of partially processed proteins. JF - Journal of Biological Chemistry AU - Piszczek, Grzegorz AU - Rozycki, Jan AU - Singh, Satyendra K AU - Ginsburg, Ann AU - Maurizi, Michael R AD - Laboratory of Biochemistry, NHLBI, and the Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/04/01/ PY - 2005 DA - 2005 Apr 01 SP - 12221 EP - 12230 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 13 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Phages KW - hexamers KW - Deletion mutant KW - Fluorescence KW - ATP KW - RepA protein KW - Titration KW - Escherichia coli KW - Calorimetry KW - Proteinase KW - Chaperones KW - Magnesium KW - pH effects KW - Phage P1 KW - ClpA protein KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17498348?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=The+Molecular+Chaperone%2C+ClpA%2C+Has+a+Single+High+Affinity+Peptide+Binding+Site+per+Hexamer&rft.au=Piszczek%2C+Grzegorz%3BRozycki%2C+Jan%3BSingh%2C+Satyendra+K%3BGinsburg%2C+Ann%3BMaurizi%2C+Michael+R&rft.aulast=Piszczek&rft.aufirst=Grzegorz&rft.date=2005-04-01&rft.volume=280&rft.issue=13&rft.spage=12221&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Phages; hexamers; Fluorescence; Deletion mutant; ATP; RepA protein; Titration; Calorimetry; Chaperones; Proteinase; Magnesium; pH effects; ClpA protein; Escherichia coli; Phage P1 ER - TY - JOUR T1 - Residential Herbicide Use and Risk of Non-Hodgkin Lymphoma AN - 17494550; 6269794 AB - Context: Environmental exposure to herbicides has been hypothesized to contribute to the long-term increase in non-Hodgkin lymphoma (NHL). Objective: To estimate the effects of residential herbicide exposure on NHL risk. Design: Population-based case-control study. Setting: Iowa and metropolitan Detroit, Los Angeles, and Seattle, 1998 to 2000. Participants: NHL patients ages 20 to 74 years and unaffected residents identified by random digit dialing and Medicare eligibility files. Main Outcome Measures: Computer-assisted personal interviews (1,321 cases, 1,057 controls) elicited data on herbicide use at each home occupied since 1970. Levels of 2,4-dichlorophenoxy-acetic acid and dicamba were measured in dust taken from used vacuum cleaner bags in the current home (679 cases, 510 controls who had owned at least half of their carpets for greater than or equal to 5 years). Results: Herbicide use on the lawn or garden was similar among cases and controls (adjusted relative risk, 1.02; 95% confidence interval, 0.84-1.23). Estimated risk did not increase with greater duration, frequency, or total number of applications of herbicides to the lawn, the garden, or to both combined. Risk was not elevated for respondents who applied the herbicides themselves and not for those exposed during the 1970s, 1980s, or 1990s. We detected 2,4-dichlorophenoxy-acetic acid equally often in homes of cases and controls (78%). We found dicamba in homes of 15% of cases and 20% of controls. We also found no elevation in risk among the respondents who had the highest dust levels and highest self-reported exposures. We found no consistent patterns for specific histologies. Conclusions: We found no detectable excess associated with residential exposures. Residential herbicide exposures are unlikely to explain the long-term increase in NHL. JF - Cancer Epidemiology, Biomarkers & Prevention AU - Hartge, Patricia AU - Colt, Joanne S AU - Severson, Richard K AU - Cerhan, James R AU - Cozen, Wendy AU - Camann, David AU - Zahm, Shelia Hoar AU - Davis, Scott AD - Department of Health and Human Services, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 934 EP - 937 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 4 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts KW - Risk assessment KW - Carpets KW - Vacuum KW - Herbicides KW - biomarkers KW - Lymphoma KW - Dust KW - X 24134:Pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17494550?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Residential+Herbicide+Use+and+Risk+of+Non-Hodgkin+Lymphoma&rft.au=Hartge%2C+Patricia%3BColt%2C+Joanne+S%3BSeverson%2C+Richard+K%3BCerhan%2C+James+R%3BCozen%2C+Wendy%3BCamann%2C+David%3BZahm%2C+Shelia+Hoar%3BDavis%2C+Scott&rft.aulast=Hartge&rft.aufirst=Patricia&rft.date=2005-04-01&rft.volume=14&rft.issue=4&rft.spage=934&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Herbicides; Lymphoma; Dust; Vacuum; Carpets; Risk assessment; biomarkers ER - TY - JOUR T1 - Models of Phase 1 vaccine trials: optimization of trial design to minimize risks of multiple serious adverse events AN - 17493098; 6242357 AB - A mathematical model of Phase 1 vaccine trial design was used to investigate strategies for minimizing the number of serious adverse events (SAEs) that could be encountered in the first Phase 1 trials of new vaccine formulations. For a relatively standard dose escalation trial with three dose groups each with 10 subjects, an optimal balanced between risk of more than one serious adverse event and trial design is achieved by splitting each dose group into two subgroups of three and seven. Based on the modeling, for a two vaccination, dose-escalating Phase 1 trial, a design where all subjects receive the first vaccination before any subject receives a second vaccination generally carries a lower risk of multiple serious adverse events than other designs. JF - Vaccine AU - Saul, A AD - Malaria Vaccine Development Branch/NIAID/NIH, 5460 Fishers Lane, Room 1113, Twinbrook I, Rockville, MD 20852, USA, asaul@niaid.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 3068 EP - 3075 PB - Butterworth-Heinemann, 313 Washington St. Newton MA 02158 USA VL - 23 IS - 23 SN - 0264-410X, 0264-410X KW - Health & Safety Science Abstracts; Risk Abstracts; Immunology Abstracts KW - Mathematical models KW - Risk reduction KW - Vaccination KW - Clinical trials KW - Vaccines KW - Side effects KW - F 06100:Vaccines - active immunity KW - R2 23060:Medical and environmental health KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17493098?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Vaccine&rft.atitle=Models+of+Phase+1+vaccine+trials%3A+optimization+of+trial+design+to+minimize+risks+of+multiple+serious+adverse+events&rft.au=Saul%2C+A&rft.aulast=Saul&rft.aufirst=A&rft.date=2005-04-01&rft.volume=23&rft.issue=23&rft.spage=3068&rft.isbn=&rft.btitle=&rft.title=Vaccine&rft.issn=0264410X&rft_id=info:doi/10.1016%2Fj.vaccine.2004.10.048 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Side effects; Risk reduction; Vaccines; Clinical trials; Vaccination; Mathematical models DO - http://dx.doi.org/10.1016/j.vaccine.2004.10.048 ER - TY - JOUR T1 - Increased Immunogenicity of an Anchor-Modified Tumor-Associated Antigen Is Due to the Enhanced Stability of the Peptide/MHC Complex: Implications for Vaccine Design AN - 17492843; 6267168 AB - The use of "anchor-fixed" altered peptide ligands is of considerable interest in the development of therapeutic vaccines for cancer and infectious diseases, but the mechanism by which successful altered peptide ligands elicit enhanced immunity is unclear. In this study, we have determined the crystallographic structure of a major tumor rejection Ag, gp100 sub(209-217), in complex with the HLA-A*0201 (HLA-A2) molecule, as well as the structure of a modified version of the peptide which substitutes methionine for threonine at position 2 (T2M; gp100 sub(209-2M)). The T2M-modified peptide, which is more immunogenic in vitro and in vivo, binds HLA-A2 with a similar to 9-fold greater affinity and has a similar to 7-fold slower dissociation rate at physiological temperature. Within the limit of the crystallographic data, the T2M substitution does not alter the structure of the peptide/HLA-A2 complex. Consistent with this finding, in peripheral blood from 95 human subjects, we were unable to identify higher frequencies of T cells specific for either the native or modified peptide. These data strongly support the conclusion that the greater immunogenicity of the gp100 sub(209-2M) peptide is due to the enhanced stability of the peptide/MHC complex, validating the anchor-fixing approach for generating therapeutic vaccine candidates. Thermodynamic data suggest that the enhanced stability of the T2M-modified peptide/HLA-A2 complex is attributable to the increased hydrophobicity of the modified peptide, but the gain due to hydrophobicity is offset considerably by the loss of a hydrogen bond made by the native peptide to the HLA-A2 molecule. Our findings have broad implications for the optimization of current vaccine-design strategies. JF - Journal of Immunology AU - Borbulevych, Oleg Y AU - Baxter, Tiffany K AU - Yu, Zhiya AU - Restifo, Nicholas P AU - Baker, Brian M AD - Department of Chemistry and Biochemistry and. Walther Cancer Research Center, University of Notre Dame, Notre Dame, IN 46556. National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 4812 EP - 4820 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 8 SN - 0022-1767, 0022-1767 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Temperature effects KW - Histocompatibility antigen HLA KW - Gene polymorphism KW - Major histocompatibility complex KW - Peripheral blood KW - Hydrophobicity KW - Infectious diseases KW - Hydrogen bonding KW - Immunogenicity KW - Antigen (tumor-associated) KW - Vaccines KW - W3 33365:Vaccines (other) KW - F 06150:Immunotherapy KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17492843?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Increased+Immunogenicity+of+an+Anchor-Modified+Tumor-Associated+Antigen+Is+Due+to+the+Enhanced+Stability+of+the+Peptide%2FMHC+Complex%3A+Implications+for+Vaccine+Design&rft.au=Borbulevych%2C+Oleg+Y%3BBaxter%2C+Tiffany+K%3BYu%2C+Zhiya%3BRestifo%2C+Nicholas+P%3BBaker%2C+Brian+M&rft.aulast=Borbulevych&rft.aufirst=Oleg&rft.date=2005-04-01&rft.volume=174&rft.issue=8&rft.spage=4812&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Histocompatibility antigen HLA; Vaccines; Hydrophobicity; Immunogenicity; Major histocompatibility complex; Hydrogen bonding; Antigen (tumor-associated); Gene polymorphism; Temperature effects; Peripheral blood; Infectious diseases ER - TY - JOUR T1 - A human phase 1 vaccine clinical trial of the Plasmodium falciparum malaria vaccine candidate apical membrane antigen 1 in Montanide ISA720 adjuvant AN - 17491489; 6242350 AB - A dose escalating, placebo-controlled phase 1 trial was conducted to test the safety and immunogenicity of a vaccine containing recombinant Plasmodium falciparum apical membrane antigen 1 (AMA1) formulated in Montanide ISA720. Three groups of volunteers were vaccinated intramuscularly with 5 mu g, 20 mu g or 80 mu g of AMA1, respectively, in 0.5 mL of formulation at 0, 3 and 6 months. Anti-AMA1 antibody levels and T cell stimulation indices were measured before and after each vaccination. No vaccine-related serious adverse events were recorded. Most subjects generated a mild to moderate, transient local reaction after the first vaccination. Three subjects developed a local reaction approximately 10 days following vaccination. Six of the 29 subjects seroconverted. Only one of these developed a high antibody titre. However, the interpretation of this trial was compromised by a loss of potency of the formulated vaccine during the course of the study. JF - Vaccine AU - Saul, A AU - Lawrence, G AU - Allworth, A AU - Elliott, S AU - Anderson, K AU - Rzepczyk, C AU - Martin, L B AU - Taylor, D AU - Eisen, D P AU - Irving, DO AU - Pye, D AU - Crewther, P E AU - Hodder, AN AU - Murphy, V J AU - Anders, R F AD - The Cooperative Research Centre for Vaccine Technology, Brisbane, Qld, Australia, asaul@niaid.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 3076 EP - 3083 PB - Butterworth-Heinemann, 313 Washington St. Newton MA 02158 USA VL - 23 IS - 23 SN - 0264-410X, 0264-410X KW - Biotechnology and Bioengineering Abstracts; Health & Safety Science Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Apical membrane antigen 1 KW - vaccines KW - Malaria KW - Adjuvants KW - clinical trials KW - Clinical trials KW - Lymphocytes T KW - Plasmodium falciparum KW - Vaccination KW - Antibodies KW - Immunogenicity KW - K 03086:Immunology & vaccination KW - W3 33365:Vaccines (other) KW - F 06100:Vaccines - active immunity KW - W 30965:Miscellaneous, Reviews KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17491489?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Vaccine&rft.atitle=A+human+phase+1+vaccine+clinical+trial+of+the+Plasmodium+falciparum+malaria+vaccine+candidate+apical+membrane+antigen+1+in+Montanide+ISA720+adjuvant&rft.au=Saul%2C+A%3BLawrence%2C+G%3BAllworth%2C+A%3BElliott%2C+S%3BAnderson%2C+K%3BRzepczyk%2C+C%3BMartin%2C+L+B%3BTaylor%2C+D%3BEisen%2C+D+P%3BIrving%2C+DO%3BPye%2C+D%3BCrewther%2C+P+E%3BHodder%2C+AN%3BMurphy%2C+V+J%3BAnders%2C+R+F&rft.aulast=Saul&rft.aufirst=A&rft.date=2005-04-01&rft.volume=23&rft.issue=23&rft.spage=3076&rft.isbn=&rft.btitle=&rft.title=Vaccine&rft.issn=0264410X&rft_id=info:doi/10.1016%2Fj.vaccine.2004.09.040 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Plasmodium falciparum; vaccines; clinical trials; Vaccination; Clinical trials; Antibodies; Apical membrane antigen 1; Malaria; Lymphocytes T; Immunogenicity; Adjuvants DO - http://dx.doi.org/10.1016/j.vaccine.2004.09.040 ER - TY - JOUR T1 - Epidemiology of alcohol-associated cancers AN - 17455537; 6645213 AB - Alcohol, especially in combination with smoking, is a well-established risk factor for cancers of the oral cavity and pharynx, esophagus, and larynx, with 25% to 80% of these cancers being attributable to alcohol. Rates of these cancers in the United States have been decreasing in recent years, possibly because of reductions in cigarette smoking and alcohol use. Chronic alcohol consumption has been linked with increased risk of liver cancer in epidemiologic studies. However, the rising rates of this cancer in the United States are most likely due to the increasing prevalence of chronic hepatitis B and C infections. Epidemiologic evidence has linked light to moderate intake of alcohol to cancers of the colorectum and female breast. These cancers are common in developed countries, so even small increases in risk can have important public health implications. Although results of most epidemiologic studies have provided little or no support for a causal relation between light and moderate alcohol use and risk of pancreatic cancer, a possible role of heavy alcohol consumption cannot be ruled out. Further studies of these cancers are needed to clarify the role of type of alcoholic beverage, the role of alcohol concentration, and the dose-response curve at low concentrations of alcohol. Future research also should be designed to promote the use of uniform ways to report alcohol intake and uniform measures for analysis, to include the investigation of alcohol- associated cancer risks in U.S. minority populations, to enhance experimental work to better understand the underlying mechanisms through which alcohol promotes carcinogenesis, and to develop preventive strategies. JF - Alcohol AU - Brown, Linda Morris AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, 6120 Executive Boulevard, Room 8026, MSC 7244, Bethesda, MD 20892-7244, USA, brownl@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 161 EP - 168 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 35 IS - 3 SN - 0741-8329, 0741-8329 KW - colorectal carcinoma KW - pancreas KW - Virology & AIDS Abstracts; Toxicology Abstracts; Health & Safety Science Abstracts; Risk Abstracts KW - Alcohol KW - Breast cancer KW - Cancer KW - Colorectal cancer KW - Esophageal cancer KW - Laryngeal cancer KW - Oral cancer KW - Pancreatic cancer KW - Pharyngeal cancer KW - Tobacco KW - Trends KW - Pharynx KW - Liver cancer KW - hepatitis B KW - Oral cavity KW - Public health KW - Dose-response effects KW - Risk factors KW - Cigarette smoking KW - Hepatitis B KW - alcohols KW - Ethanol KW - Esophagus KW - Alcoholic beverages KW - Breast KW - Light effects KW - Epidemiology KW - Carcinogenesis KW - Chronic infection KW - Liver KW - Larynx KW - H 11000:Diseases/Injuries/Trauma KW - R2 23060:Medical and environmental health KW - X 24180:Social poisons & drug abuse KW - V 22400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17455537?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcohol&rft.atitle=Epidemiology+of+alcohol-associated+cancers&rft.au=Brown%2C+Linda+Morris&rft.aulast=Brown&rft.aufirst=Linda&rft.date=2005-04-01&rft.volume=35&rft.issue=3&rft.spage=161&rft.isbn=&rft.btitle=&rft.title=Alcohol&rft.issn=07418329&rft_id=info:doi/10.1016%2Fj.alcohol.2005.03.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Esophagus; Pharynx; Alcoholic beverages; Liver cancer; Breast; Pancreatic cancer; Oral cavity; Light effects; Public health; Epidemiology; Risk factors; Chronic infection; Cigarette smoking; Carcinogenesis; Larynx; alcohols; Hepatitis B; Breast cancer; Ethanol; Alcohol; Dose-response effects; Liver; hepatitis B; Cancer DO - http://dx.doi.org/10.1016/j.alcohol.2005.03.008 ER - TY - JOUR T1 - DNA adducts from acetaldehyde: implications for alcohol-related carcinogenesis AN - 17451956; 6645210 AB - Alcoholic beverage consumption is classified as a known human carcinogen, causally related to an increased risk of cancer of the upper gastrointestinal tract. The formation of acetaldehyde from ethanol metabolism seems to be the major mechanism underlying this effect. Acetaldehyde is carcinogenic in rodents and causes sister chromatid exchanges and chromosomal aberrations in human cells. The best-studied DNA adduct from acetaldehyde is N super(2)-ethyl-2'- deoxyguanosine, which is increased in liver DNA obtained from ethanol-treated rodents and in white blood cells obtained from human alcohol abusers. However, the carcinogenic relevance of this adduct is unclear in view of the lack of evidence that it is mutagenic in mammalian cells. A different DNA adduct, 1,N super(2)-propano-2'-deoxyguanosine (PdG), can also be formed from acetaldehyde in the presence of histones and other basic molecules. PdG has been shown to be responsible for the genotoxic and mutagenic effects of crotonaldehyde. The PdG adduct can exist in either of two forms: a ring-closed form or a ring-opened aldehyde form. Whereas the ring-closed form is mutagenic, the aldehyde form can participate in the formation of secondary lesions, including DNA-protein cross-links and DNA interstrand cross-links. The formation of these types of complex secondary DNA lesions resulting from PdG may explain many of the observed genotoxic effects of acetaldehyde described above. Repair of PdG and its associated adducts is complex, involving multiple pathways. Inherited variation in the genes encoding the proteins involved in the repair of PdG and its secondary adducts may contribute to susceptibility to alcoholic beverage-related carcinogenesis. JF - Alcohol AU - Brooks, Philip J AU - Theruvathu, Jacob A AD - Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Room 3S32, MSC 9412, Bethesda, MD 20892-9412, USA, pjbrooks@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 187 EP - 193 PB - Elsevier Science Inc., Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 35 IS - 3 SN - 0741-8329, 0741-8329 KW - Biochemistry Abstracts 2: Nucleic Acids; Toxicology Abstracts KW - Crotonaldehyde KW - Acetaldehyde KW - DNA-protein cross-links KW - DNA interstrand cross-links KW - DNA repair KW - Fanconi anemia KW - Xeroderma pigmentosum KW - DNA adducts KW - Alcoholic beverages KW - Histones KW - Genotoxicity KW - Leukocytes KW - sister chromatids KW - Carcinogens KW - Deoxyguanosine KW - Cancer KW - Alcoholics KW - Digestive tract KW - Mammalian cells KW - Carcinogenesis KW - Liver KW - Gastrointestinal tract KW - Aldehydes KW - Chromosome aberrations KW - Metabolism KW - Ethanol KW - N 14820:DNA Metabolism & Structure KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17451956?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcohol&rft.atitle=DNA+adducts+from+acetaldehyde%3A+implications+for+alcohol-related+carcinogenesis&rft.au=Brooks%2C+Philip+J%3BTheruvathu%2C+Jacob+A&rft.aulast=Brooks&rft.aufirst=Philip&rft.date=2005-04-01&rft.volume=35&rft.issue=3&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Alcohol&rft.issn=07418329&rft_id=info:doi/10.1016%2Fj.alcohol.2005.03.009 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - DNA adducts; Histones; Alcoholic beverages; Acetaldehyde; Leukocytes; Genotoxicity; sister chromatids; Carcinogens; Deoxyguanosine; Alcoholics; Cancer; Digestive tract; Mammalian cells; Carcinogenesis; Liver; Gastrointestinal tract; Aldehydes; Chromosome aberrations; Metabolism; Ethanol DO - http://dx.doi.org/10.1016/j.alcohol.2005.03.009 ER - TY - JOUR T1 - Nicotine induces conditioned place preferences over a large range of doses in rats AN - 17395037; 6484030 AB - Rationale: Conditioned place preference (CPP) procedures provide one measure of potential rewarding effects of abused drugs. Many attempts to induce CPP with nicotine have been unsuccessful. Objectives: To assess the influence of nicotine dose and stimulus assignment procedure on development of nicotine-induced CPP. Methods: Initial preferences for one side of a two-compartment apparatus were first determined in Sprague-Dawley rats. In subsequent conditioning trials, the compartment paired with nicotine was the initially preferred side for half of the rats, and the initially non-preferred side for the other half. Rats received either an injection of nicotine (0.01-2 mg/kg SC) before being placed in one compartment (three trials) or saline before being placed in the other compartment (three trials). Control rats had saline injections associated with both compartments. A final test trial with no injection assessed final place preference. Results: Significant CPP were induced by 0.1-1.4 mg/kg doses of nicotine. Nicotine-induced CPP were only apparent when nicotine was paired with the initially non-preferred side. Moreover, a very high dose of nicotine (2 mg/kg) induced conditioned place aversion when paired with the initially preferred side of the apparatus. Conclusions: Nicotine induced significant CPP across a wide range of doses, in accordance with its role as the primary addictive component of tobacco. Small preferences for one side of the apparatus played a major role in the development of nicotine-induced CPP. These findings suggest that biased procedures may be more suitable than unbiased procedures for evaluation of rewarding effects of nicotine using CPP paradigms. JF - Psychopharmacology AU - Le Foll, Bernard AU - Goldberg, Steven R AD - Department of Health and Human Services, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA, blefoll@intra.nida.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 481 EP - 492 PB - Springer-Verlag (Berlin), Heidelberger Platz 3 Berlin 14197 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 178 IS - 4 SN - 0033-3158, 0033-3158 KW - CSA Neurosciences Abstracts; Animal Behavior Abstracts; Toxicology Abstracts KW - Place preferences KW - Dose dependency KW - Nicotine KW - Aversion KW - Reinforcement KW - Tobacco KW - Drug abuse KW - N3 11047:Neuropsychobiology KW - X 24180:Social poisons & drug abuse KW - Y 25817:Mammals (excluding primates) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17395037?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Adoptive+cell+transfer+therapy+following+non-myeloablative+but+lymphodepleting+chemotherapy+for+the+treatment+of+patients+with+refractory+metastatic+melanoma.&rft.au=Dudley%2C+Mark+E%3BWunderlich%2C+John+R%3BYang%2C+James+C%3BSherry%2C+Richard+M%3BTopalian%2C+Suzanne+L%3BRestifo%2C+Nicholas+P%3BRoyal%2C+Richard+E%3BKammula%2C+Udai%3BWhite%2C+Don+E%3BMavroukakis%2C+Sharon+A%3BRogers%2C+Linda+J%3BGracia%2C+Gerald+J%3BJones%2C+Stephanie+A%3BMangiameli%2C+David+P%3BPelletier%2C+Michelle+M%3BGea-Banacloche%2C+Juan%3BRobinson%2C+Michael+R%3BBerman%2C+David+M%3BFilie%2C+Armando+C%3BAbati%2C+Andrea%3BRosenberg%2C+Steven+A&rft.aulast=Dudley&rft.aufirst=Mark&rft.date=2005-04-01&rft.volume=23&rft.issue=10&rft.spage=2346&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Dose dependency; Place preferences; Nicotine; Aversion; Tobacco; Reinforcement; Drug abuse DO - http://dx.doi.org/10.1007/s00213-004-2021-5 ER - TY - JOUR T1 - Modeling a description logic vocabulary for cancer research AN - 17367073; 6444901 AB - The National Cancer Institute has developed the NCI Thesaurus, a biomedical vocabulary for cancer research, covering terminology across a wide range of cancer research domains. A major design goal of the NCI Thesaurus is to facilitate translational research. We describe: the features of Ontylog, a description logic used to build NCI Thesaurus; our methodology for enhancing the terminology through collaboration between ontologists and domain experts, and for addressing certain real world challenges arising in modeling the Thesaurus; and finally, we describe the conversion of NCI Thesaurus from Ontylog into Web Ontology Language Lite. Ontylog has proven well suited for constructing big biomedical vocabularies. We have capitalized on the Ontylog constructs Kind and Role in the collaboration process described in this paper to facilitate communication between ontologists and domain experts. The artifacts and processes developed by NCI for collaboration may be useful in other biomedical terminology development efforts. JF - Journal of Biomedical Informatics AU - Hartel, F W AU - De Coronado, S AU - Dionne, R AU - Fragoso, G AU - Golbeck, J AD - National Cancer Institute Center for Bioinformatics, 6116 Executive Blvd, Suite 403, Bethesda, MD 20892-8335, United States, decorons@mail.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 114 EP - 129 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 38 IS - 2 SN - 1532-0464, 1532-0464 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Translation KW - Communication KW - Language KW - Bioinformatics KW - Cancer KW - W4 140:Bioinformatics & Computers in Health & Medicine KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17367073?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biomedical+Informatics&rft.atitle=Modeling+a+description+logic+vocabulary+for+cancer+research&rft.au=Hartel%2C+F+W%3BDe+Coronado%2C+S%3BDionne%2C+R%3BFragoso%2C+G%3BGolbeck%2C+J&rft.aulast=Hartel&rft.aufirst=F&rft.date=2005-04-01&rft.volume=38&rft.issue=2&rft.spage=114&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biomedical+Informatics&rft.issn=15320464&rft_id=info:doi/10.1016%2Fj.jbi.2004.09.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; Language; Translation; Communication; Bioinformatics DO - http://dx.doi.org/10.1016/j.jbi.2004.09.001 ER - TY - JOUR T1 - Initial Ocular Following in Humans Depends Critically on the Fourier Components of the Motion Stimulus AN - 1513504645; 19284763 AB - Abstract: Visual motion is sensed by low-level (energy-based) and high-level (feature-based) mechanisms. Our interest is in the motion detectors underlying the initial ocular following responses (OFR) that are elicited at ultrashort latencies by sudden motions of large images. OFR were elicited in humans by applying horizontal motion to vertical square-wave gratings lacking the fundamental. In the frequency domain, a pure square wave is composed of the odd harmonics-first, third, fifth, seventh, etc.-such that the third, fifth, seventh, etc., have amplitudes that are one-third, one-fifth, one-seventh, etc., that of the first, and the missing fundamental stimulus lacks the first harmonic. Motion consisted of successive quarter-wavelength steps, so the features and 4n+1 harmonics (where n= integer) shifted forward, whereas the 4n-1 harmonics-including the strongest Fourier component (the third harmonic)-shifted backward (spatial aliasing). Thus, the net Fourier energy and the non-Fourier features moved in opposite directions. Initial OFR, recorded with the search coil technique, had minimum latencies of 60 to 70 ms and were always in the direction of the third harmonic, for example, leftward steps resulted in rightward OFR. Thus, the earliest OFR were strongly dependent on the motion of the major Fourier component, consistent with mediation by oriented spatiotemporal visual filters as in the well-known energy model of motion detection. Introducing interstimulus intervals of 10 to 100 ms (during which the screen was uniform gray) reversed the initial direction of tracking, consistent with extensive neurophysiological and psychophysical data suggesting that the visual input to the motion detectors has a biphasic temporal impulse response. JF - Annals of the New York Academy of Sciences AU - Chen, K J AU - Sheliga, B M AU - Fitzgibbon, E J AU - Miles, F A AD - Laboratory of Sensorimotor Research, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-4435, USA Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 260 EP - 271 PB - Wiley-Blackwell, 111 River Street Hoboken NJ 07030-5774 United States VL - 1039 IS - 1 SN - 0077-8923, 0077-8923 KW - Solid State and Superconductivity Abstracts (SO); Environmental Engineering Abstracts (EN); Electronics and Communications Abstracts (EA) KW - Harmonics KW - Frequency domains KW - Human KW - Fourier analysis KW - Detectors KW - Image detection KW - Searching KW - Visual UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1513504645?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aenvironmentalengabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.atitle=Initial+Ocular+Following+in+Humans+Depends+Critically+on+the+Fourier+Components+of+the+Motion+Stimulus&rft.au=Chen%2C+K+J%3BSheliga%2C+B+M%3BFitzgibbon%2C+E+J%3BMiles%2C+F+A&rft.aulast=Chen&rft.aufirst=K&rft.date=2005-04-01&rft.volume=1039&rft.issue=1&rft.spage=260&rft.isbn=&rft.btitle=&rft.title=Annals+of+the+New+York+Academy+of+Sciences&rft.issn=00778923&rft_id=info:doi/10.1196%2Fannals.1325.025 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2014-04-01 N1 - Document feature - figure 5 N1 - Last updated - 2014-06-02 DO - http://dx.doi.org/10.1196/annals.1325.025 ER - TY - JOUR T1 - Cancer risk associated with receipt of vaccines contaminated with simian virus 40: epidemiologic research AN - 1028021801; 16614491 AB - Simian virus (SV)40 was an accidental contaminant of poliovirus vaccines used widely in the USA and other countries in 1955-1962. Exposure to SV40 via contaminated vaccines has led to concern as SV40 causes cancer in laboratory animals. In addition, some laboratories, although not all, have detected SV40 DNA in human tumors including mesothelioma, certain brain tumors, osteosarcoma and non-Hodgkin''s lymphoma. This article reviews the data regarding contamination of poliovirus vaccines with SV40 and summarizes the results from epidemiologic studies of vaccine recipients. Long-term follow-up studies have not revealed recipients of SV40-contaminated poliovirus vaccines to be at an increased risk for cancer. Thus, these studies are somewhat reassuring and indicate that either SV40 does not readily infect humans or, following infection, does not cause cancer. Recognizing that the history of SV40 contamination of vaccines highlights an inherent risk of contamination of vaccines with adventitious agents, the Institute of Medicine recently called for the development of a comprehensive US plan to prevent vaccine contamination and respond to potential contamination events when they arise. JF - Expert Review of Vaccines AU - Engels, Eric A AD - National Cancer InstituteViral Epidemiology Branch, Division of Cancer Epidemiology and Genetics, DHHS, 6120 Executive Blvd, EPS 8010Rockville, MD 20892USA, engelse@exchange.nih.gov Y1 - 2005/04// PY - 2005 DA - Apr 2005 SP - 197 EP - 206 PB - Future Science Group (FSG), Unitec House, 2 Albert Place London N3 1QB United Kingdom VL - 4 IS - 2 SN - 1476-0584, 1476-0584 KW - Virology & AIDS Abstracts; Risk Abstracts; Health & Safety Science Abstracts KW - Brain tumors KW - Cancer KW - Contaminants KW - Contamination KW - DNA KW - Data processing KW - Historical account KW - Infection KW - Laboratory animals KW - Lymphoma KW - Osteosarcoma KW - Poliovirus KW - Reviews KW - Risk factors KW - Tumors KW - Vaccines KW - mesothelioma KW - vaccines KW - Simian virus 40 KW - USA KW - R2 23060:Medical and environmental health KW - H 4000:Food and Drugs KW - V 22370:Oncology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1028021801?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Maintenance+of+Pulmonary+Th1+Effector+Function+in+Chronic+Tuberculosis+Requires+Persistent+IL-12+Production&rft.au=Feng%2C+Carl+G%3BJankovic%2C+Dragana%3BKullberg%2C+Marika%3BCheever%2C+Allen%3BScanga%2C+Charles+A%3BHieny%2C+Sara%3BCaspar%2C+Patricia%3BYap%2C+George+S%3BSher%2C+Alan&rft.aulast=Feng&rft.aufirst=Carl&rft.date=2005-04-01&rft.volume=174&rft.issue=7&rft.spage=4185&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2012-07-01 N1 - Number of references - 55 N1 - Last updated - 2013-01-11 N1 - SubjectsTermNotLitGenreText - Data processing; Contamination; Laboratory animals; Osteosarcoma; Infection; Cancer; Brain tumors; Reviews; Risk factors; DNA; mesothelioma; Vaccines; Contaminants; Lymphoma; Historical account; Poliovirus; vaccines; Tumors; Simian virus 40; USA DO - http://dx.doi.org/10.1586/14760584.4.2.197 ER - TY - JOUR T1 - Solution Structure of Enzyme IIA super(Chitobiose) from the N,N'-Diacetylchitobiose Branch of the Escherichia coli Phosphotransferase System AN - 17482529; 6189660 AB - The solution structure of trimeric Escherichia coli enzyme IIA super(Chb) (34 kDa), a component of the N,N'-diacetylchitobiose/lactose branch of the phosphotransferase signal transduction system, has been determined by NMR spectroscopy. Backbone residual dipolar couplings were used to provide long range orientational restraints, and long range (|i -j| => 5 residues) nuclear Overhauser enhancement restraints were derived exclusively from samples in which at least one subunit was super(15)N/ super(13)C/ super(2)H/(Val-Leu-Ile)-methyl-protonated. Each subunit consists of a three-helix bundle. Hydrophobic residues lining helix 3 of each subunit are largely responsible for the formation of a parallel coiled-coil trimer. The active site histidines (His-89 from each subunit) are located in three symmetrically placed deep crevices located at the interface of two adjacent subunits (A and C, C and B, and B and A). Partially shielded from bulk solvent, structural modeling suggests that phosphorylated His-89 is stabilized by electrostatic interactions with the side chains of His-93 from the same subunit and Gln-91 from the adjacent subunit. Comparison with the x-ray structure of Lactobacillus lactis IIA super(Lac) reveals some substantial structural differences, particularly in regard to helix 3, which exhibits a 40 degree kink in IIA super(Lac) versus a 7 degree bend in IIA super(Chb). This is associated with the presence of an unusually large (230-Aa super(3)) buried hydrophobic cavity at the trimer interface in IIA super(Lac) that is reduced to only 45 Aa super(3) in IIA super(Chb). JF - Journal of Biological Chemistry AU - Tang, Chun AU - Williams, David C AU - Ghirlando, Rodolfo AU - Clore, GMarius AD - Laboratories of Chemical Physics and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland Y1 - 2005/03/25/ PY - 2005 DA - 2005 Mar 25 SP - 11770 EP - 11780 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 12 SN - 0021-9258, 0021-9258 KW - chitobiose KW - N,N'-diacetylchitobiose KW - Microbiology Abstracts B: Bacteriology KW - Lactose KW - Magnetic resonance spectroscopy KW - Histidine KW - Lactobacillus lactis KW - Escherichia coli KW - Hydrophobicity KW - phosphotransferase KW - Signal transduction KW - J 02728:Enzymes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17482529?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Solution+Structure+of+Enzyme+IIA+super%28Chitobiose%29+from+the+N%2CN%27-Diacetylchitobiose+Branch+of+the+Escherichia+coli+Phosphotransferase+System&rft.au=Tang%2C+Chun%3BWilliams%2C+David+C%3BGhirlando%2C+Rodolfo%3BClore%2C+GMarius&rft.aulast=Tang&rft.aufirst=Chun&rft.date=2005-03-25&rft.volume=280&rft.issue=12&rft.spage=11770&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Lactobacillus lactis; Hydrophobicity; phosphotransferase; Histidine; Magnetic resonance spectroscopy; Signal transduction; Lactose ER - TY - JOUR T1 - Compliance and toxicity of adjuvant CMF in elderly breast cancer patients: a single-center experience. AN - 67741415; 15790416 AB - Few data are available on compliance and safety of adjuvant chemotherapy when indicated in elderly breast cancer patients; CMF (cyclophosphamide, methotrexate, fluorouracil) can be reasonably considered the most widely accepted standard of treatment. We retrospectively reviewed compliance and safety of adjuvant CMF in patients older than 60. The treatment was indicated if patients had no severe comorbidity, a high-risk of recurrence, and were younger than 75. Toxicity was coded by NCI-CTC. Toxicity and compliance were compared between two age subgroups ( or = 65) by Fisher exact test and exact Wilcoxon rank-sum test. From March 1991 to March 2002, 180 patients were identified, 100 older than 60 and younger than 65, and 80 aged 65 or older. Febrile neutropenia was more frequent among older patients (p = 0.05). Leukopenia, neutropenia, nausea, cardiac toxicity and thrombophlebitis tended to be more frequent or severe among elderlies, while mucositis tended to be more evident among younger patients, all not significantly. Almost one half (47%) of the older patients receiving concomitant radiotherapy experienced grade 3-4 haematological toxicity. Compliance was similar in the two groups, with 6 cycles administered in 86% and 79%, day-8 chemotherapy omitted at least once in 36% and 39%, dose reduction in 27% and 38%, prolonged treatment duration (> or = 29 weeks) in 10% and 11% and need of G-CSF in 9% and 18%, among younger and older patients, respectively. Our data show that, in a highly selected population of patients 65 or more years old, CMF is as feasible as in patients older than 60 and younger than 65, but with a relevant burden of toxicity. We suggest that prospective trials in elderly patients testing less toxic treatment schemes are mandatory before indicating adjuvant chemotherapy to all elderly patients with significant risk of breast cancer recurrence. JF - BMC cancer AU - De Maio, Ermelinda AU - Gravina, Adriano AU - Pacilio, Carmen AU - Amabile, Gerardo AU - Labonia, Vincenzo AU - Landi, Gabriella AU - Nuzzo, Francesco AU - Rossi, Emanuela AU - D'Aiuto, Giuseppe AU - Capasso, Immacolata AU - Rinaldo, Massimo AU - Morrica, Brunello AU - Elmo, Massimo AU - Di Maio, Massimo AU - Perrone, Francesco AU - de Matteis, Andrea AD - Clinical Trials Unit, National Cancer Institute, Naples, Italy. e.demaio@unina.it Y1 - 2005/03/24/ PY - 2005 DA - 2005 Mar 24 SP - 30 VL - 5 KW - Cyclophosphamide KW - 8N3DW7272P KW - Fluorouracil KW - U3P01618RT KW - Methotrexate KW - YL5FZ2Y5U1 KW - Index Medicus KW - Disease-Free Survival KW - Humans KW - Retrospective Studies KW - Aged KW - Cyclophosphamide -- adverse effects KW - Fluorouracil -- therapeutic use KW - Fluorouracil -- adverse effects KW - Hematologic Diseases -- chemically induced KW - Radiotherapy, Adjuvant KW - Cyclophosphamide -- therapeutic use KW - Methotrexate -- adverse effects KW - Methotrexate -- therapeutic use KW - Middle Aged KW - Chemotherapy, Adjuvant KW - Female KW - Breast Neoplasms -- drug therapy KW - Patient Compliance KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Breast Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67741415?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+cancer&rft.atitle=Compliance+and+toxicity+of+adjuvant+CMF+in+elderly+breast+cancer+patients%3A+a+single-center+experience.&rft.au=De+Maio%2C+Ermelinda%3BGravina%2C+Adriano%3BPacilio%2C+Carmen%3BAmabile%2C+Gerardo%3BLabonia%2C+Vincenzo%3BLandi%2C+Gabriella%3BNuzzo%2C+Francesco%3BRossi%2C+Emanuela%3BD%27Aiuto%2C+Giuseppe%3BCapasso%2C+Immacolata%3BRinaldo%2C+Massimo%3BMorrica%2C+Brunello%3BElmo%2C+Massimo%3BDi+Maio%2C+Massimo%3BPerrone%2C+Francesco%3Bde+Matteis%2C+Andrea&rft.aulast=De+Maio&rft.aufirst=Ermelinda&rft.date=2005-03-24&rft.volume=5&rft.issue=&rft.spage=30&rft.isbn=&rft.btitle=&rft.title=BMC+cancer&rft.issn=1471-2407&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-04-15 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Clin Oncol. 2001 Aug 1;19(15):3500-5 [11481356] J Natl Cancer Inst. 2001 Jul 4;93(13):979-89 [11438563] J Natl Cancer Inst Monogr. 2001;(30):146-52 [11773309] Lancet Oncol. 2001 Nov;2(11):691-7 [11902540] Cancer. 2002 Sep 1;95(5):989-96 [12209681] Br J Surg. 2003 Apr;90(4):388-99 [12673739] J Clin Oncol. 2003 Apr 15;21(8):1431-9 [12668651] Cancer. 2003 May 1;97(9):2150-9 [12712466] J Clin Oncol. 2003 Sep 1;21(17):3357-65 [12847142] J Clin Oncol. 2003 Sep 1;21(17):3214-9 [12874269] Cancer. 2003 Sep 15;98(6):1141-9 [12973837] J Clin Oncol. 2003 Oct 1;21(19):3580-7 [12913099] Ann Oncol. 2004 Feb;15(2):207-10 [14760110] J Natl Cancer Inst. 1996 Nov 6;88(21):1571-9 [8901855] J Clin Oncol. 1998 Aug;16(8):2651-8 [9704715] J Natl Cancer Inst. 1998 Nov 4;90(21):1601-8 [9811309] Lancet. 1999 Jul 10;354(9173):130-1 [10408495] J Clin Oncol. 2004 Dec 1;22(23):4622-30 [15505276] N Engl J Med. 1999 Dec 30;341(27):2061-7 [10615079] J Clin Oncol. 2000 Apr;18(7):1412-22 [10735888] J Clin Oncol. 2000 Apr;18(8):1709-17 [10764431] Cancer. 2000 Aug 1;89(3):561-73 [10931455] J Clin Oncol. 2001 Jan 1;19(1):220-30 [11134216] J Clin Oncol. 2001 Feb 1;19(3):602-11 [11157009] J Clin Oncol. 2001 Sep 15;19(18):3817-27 [11559719] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitrite infusions to prevent delayed cerebral vasospasm in a primate model of subarachnoid hemorrhage. AN - 67539289; 15784871 AB - Delayed cerebral vasospasm causes permanent neurological deficits or death in at least 15% of patients following otherwise successful treatment for ruptured intracranial aneurysm. Decreased bioavailability of nitric oxide has been associated with the development of cerebral vasospasm. To determine whether infusions of nitrite will prevent delayed cerebral vasospasm. A total of 14 anesthetized cynomolgus monkeys had an autologous blood clot placed around the right middle cerebral artery. Cerebral arteriography was performed before clot placement and on days 7 and 14 to assess vasospasm. The study was conducted from August 2003 to February 2004. A 90-mg sodium nitrite intravenous solution infused over 24 hours plus a 45-mg sodium nitrite bolus daily (n = 3); a 180-mg sodium nitrite intravenous solution infused over 24 hours (n = 3); or a control saline solution infusion (n = 8). Each was infused continuously for 14 days. Nitrite, S-nitrosothiol, and methemoglobin levels in blood and cerebrospinal fluid and degree of arteriographic vasospasm. In control monkeys, mean (SD) cerebrospinal fluid nitrite levels decreased from 3.1 (1.5) micromol/L to 0.4 (0.1) micromol/L at day 7 and to 0.4 (0.4) micromol/L at day 14 (P = .03). All 8 control monkeys developed significant vasospasm of the right middle cerebral artery, which was complicated by stroke and death in 1 animal. Sodium nitrite infusions increased the nitrite and methemoglobin levels (<2.1% of total hemoglobin) in the blood and cerebrospinal fluid without evoking systemic hypotension. Nitrite infusion prevented development of vasospasm (no animals developed significant vasospasm; mean [SD] reduction in right middle cerebral artery area on day 7 after subarachnoid hemorrhage of 8% [9%] in nitrite-treated monkeys vs 47% [5%] in saline-treated controls; P<.001). There was a negative correlation between the concentration of nitrite in cerebrospinal fluid and the degree of cerebral vasospasm (P<.001). Pharmacological effects of nitrite infusion were also associated with the formation of S-nitrosothiol in cerebrospinal fluid. There was no clinical or pathological evidence of nitrite toxicity. Subacute sodium nitrite infusions prevented delayed cerebral vasospasm in a primate model of subarachnoid hemorrhage. JF - JAMA AU - Pluta, Ryszard M AU - Dejam, Andre AU - Grimes, George AU - Gladwin, Mark T AU - Oldfield, Edward H AD - Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Md 20892, USA. rysiek@ninds.nih.gov Y1 - 2005/03/23/ PY - 2005 DA - 2005 Mar 23 SP - 1477 EP - 1484 VL - 293 IS - 12 KW - Nitrites KW - 0 KW - S-Nitrosothiols KW - Methemoglobin KW - 9008-37-1 KW - Sodium Nitrite KW - M0KG633D4F KW - Abridged Index Medicus KW - Index Medicus KW - Nitrites -- blood KW - Animals KW - Cerebral Angiography KW - Macaca fascicularis KW - Infusions, Intravenous KW - Infarction, Middle Cerebral Artery KW - Nitrites -- cerebrospinal fluid KW - Disease Models, Animal KW - S-Nitrosothiols -- cerebrospinal fluid KW - S-Nitrosothiols -- blood KW - Methemoglobin -- analysis KW - Sodium Nitrite -- administration & dosage KW - Vasospasm, Intracranial -- prevention & control KW - Vasospasm, Intracranial -- diagnostic imaging KW - Sodium Nitrite -- therapeutic use KW - Vasospasm, Intracranial -- metabolism KW - Vasospasm, Intracranial -- etiology KW - Subarachnoid Hemorrhage -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67539289?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=JAMA&rft.atitle=Nitrite+infusions+to+prevent+delayed+cerebral+vasospasm+in+a+primate+model+of+subarachnoid+hemorrhage.&rft.au=Pluta%2C+Ryszard+M%3BDejam%2C+Andre%3BGrimes%2C+George%3BGladwin%2C+Mark+T%3BOldfield%2C+Edward+H&rft.aulast=Pluta&rft.aufirst=Ryszard&rft.date=2005-03-23&rft.volume=293&rft.issue=12&rft.spage=1477&rft.isbn=&rft.btitle=&rft.title=JAMA&rft.issn=1538-3598&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-29 N1 - Date created - 2005-03-23 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: JAMA. 2005 Jul 6;294(1):40; author reply 40-1 [15998885] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Intramolecular interaction between phosphorylated tyrosine-783 and the C-terminal Src homology 2 domain activates phospholipase C-gamma1. AN - 67546745; 15764700 AB - Phospholipase C-gamma1 (PLC-gamma1) contains two tandem Src homology 2 (SH2) domains. The NH(2)-terminal SH2 domain has been known to mediate the binding of PLC-gamma1 to receptor protein tyrosine kinases, which then activate PLC-gamma1 via phosphorylation at Y783. We now show that the phosphorylated Y783 residue (pY783) associates with the COOH-terminal SH2 domain [SH2(C)] within the same molecule of PLC-gamma1. The specificity of this intramolecular interaction is demonstrated in several ways. The mutation of SH2(C), but not of the NH(2)-terminal SH2 domain, exposes pY783 and makes it available for binding by anti-pY783 antibodies, for intermolecular association with a GST fusion protein containing the tandem SH2 domains of PLC-gamma1 and for dephosphorylation by phosphatases. The intramolecular interaction between pY783 and SH2(C) induces a rearrangement of surface charge such that PLC-gamma1 molecules phosphorylated at Y783 are retained more strongly by heparin resins than are unphosphorylated molecules. Finally, the intramolecular interaction of pY783 with SH2(C) results in activation of phospholipase activity. Our results thus clarify the molecular mechanism of PLC-gamma1 activation, revealing the specific function of pY783 and the distinct roles of the two SH2 domains in this process. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Poulin, Benoit AU - Sekiya, Fujio AU - Rhee, Sue Goo AD - Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8015, USA. Y1 - 2005/03/22/ PY - 2005 DA - 2005 Mar 22 SP - 4276 EP - 4281 VL - 102 IS - 12 SN - 0027-8424, 0027-8424 KW - Recombinant Proteins KW - 0 KW - Tyrosine KW - 42HK56048U KW - Type C Phospholipases KW - EC 3.1.4.- KW - Phospholipase C gamma KW - EC 3.1.4.3 KW - Index Medicus KW - Animals KW - Enzyme Activation KW - Recombinant Proteins -- genetics KW - Models, Biological KW - src Homology Domains KW - Binding Sites KW - Tyrosine -- chemistry KW - Mutagenesis, Site-Directed KW - Rats KW - Phosphorylation KW - Recombinant Proteins -- metabolism KW - In Vitro Techniques KW - Recombinant Proteins -- chemistry KW - Protein Conformation KW - Type C Phospholipases -- chemistry KW - Type C Phospholipases -- genetics KW - Type C Phospholipases -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67546745?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Intramolecular+interaction+between+phosphorylated+tyrosine-783+and+the+C-terminal+Src+homology+2+domain+activates+phospholipase+C-gamma1.&rft.au=Poulin%2C+Benoit%3BSekiya%2C+Fujio%3BRhee%2C+Sue+Goo&rft.aulast=Poulin&rft.aufirst=Benoit&rft.date=2005-03-22&rft.volume=102&rft.issue=12&rft.spage=4276&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-03-23 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1999 Sep 10;274(37):26091-7 [10473558] J Immunol. 1999 Aug 15;163(4):1746-9 [10438904] Exp Cell Res. 1999 Nov 25;253(1):15-24 [10579907] J Biol Chem. 2000 Mar 3;275(9):6411-6 [10692443] Annu Rev Biochem. 2001;70:281-312 [11395409] Development. 2002 Aug;129(15):3533-44 [12117804] J Biol Chem. 2004 Jul 30;279(31):32181-90 [15161916] Mol Cell Biol. 2004 Nov;24(22):9986-99 [15509800] Mol Cell Biol. 1985 Dec;5(12):3403-9 [3939316] J Biol Chem. 1988 Oct 5;263(28):14497-504 [2459119] J Biol Chem. 1990 Mar 5;265(7):3940-3 [1689310] J Biol Chem. 1990 Mar 5;265(7):3944-8 [1689311] Science. 1990 Nov 30;250(4985):1253-6 [1700866] Cell. 1991 May 3;65(3):435-41 [1708307] J Biol Chem. 1992 May 15;267(14):9678-83 [1315766] J Biol Chem. 1992 May 25;267(15):10447-56 [1316902] J Biol Chem. 1992 Aug 15;267(23):16048-55 [1644792] Cell. 1994 May 6;77(3):461-72 [8181064] Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2999-3003 [9096335] J Biol Chem. 1998 Jan 9;273(2):729-35 [9422724] EMBO J. 1998 Jan 15;17(2):414-22 [9430633] J Biol Chem. 1998 Feb 20;273(8):4465-9 [9468499] J Immunol. 1998 Feb 1;160(3):1059-66 [9570517] Mol Cell Biol. 1999 Jul;19(7):4961-70 [10373546] Mol Cell Biol. 1999 Nov;19(11):7388-98 [10523627] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - HIF-1alpha induces genetic instability by transcriptionally downregulating MutSalpha expression. AN - 67533941; 15780936 AB - Hypoxia promotes genetic instability by undefined mechanisms. The transcription factor HIF-1alpha is crucial for the cellular response to hypoxia and is frequently overexpressed in human cancers, resulting in the activation of genes essential for cell survival. Here, we demonstrate that HIF-1alpha is responsible for genetic instability at the nucleotide level by inhibiting MSH2 and MSH6, thereby decreasing levels of the MSH2-MSH6 complex, MutSalpha, which recognizes base mismatches. HIF-1alpha displaces the transcriptional activator Myc from Sp1 binding to repress MutSalpha expression in a p53-dependent manner; Sp1 serves as a molecular switch by recruiting HIF-1alpha to the gene promoter under hypoxia. Furthermore, in human sporadic colon cancers, HIF-1alpha overexpression is statistically associated with the loss of MSH2 expression, especially when p53 is immunochemically undetectable. These findings indicate that the regulation of DNA repair is an integral part of the hypoxic response, providing molecular insights into the mechanisms underlying hypoxia-induced genetic instability. JF - Molecular cell AU - Koshiji, Minori AU - To, Kenneth K-W AU - Hammer, Stefanie AU - Kumamoto, Kensuke AU - Harris, Adrian L AU - Modrich, Paul AU - Huang, L Eric AD - Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/03/18/ PY - 2005 DA - 2005 Mar 18 SP - 793 EP - 803 VL - 17 IS - 6 SN - 1097-2765, 1097-2765 KW - DNA-Binding Proteins KW - 0 KW - G-T mismatch-binding protein KW - HIF1A protein, human KW - Hypoxia-Inducible Factor 1, alpha Subunit KW - MYC protein, human KW - Proto-Oncogene Proteins KW - Proto-Oncogene Proteins c-myc KW - Sp1 Transcription Factor KW - Transcription Factors KW - Tumor Suppressor Protein p53 KW - MSH2 protein, human KW - EC 3.6.1.3 KW - MutS Homolog 2 Protein KW - Index Medicus KW - Sp1 Transcription Factor -- genetics KW - Colonic Neoplasms -- genetics KW - DNA Repair KW - Humans KW - Proto-Oncogene Proteins c-myc -- genetics KW - Proto-Oncogene Proteins c-myc -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism KW - Protein Binding KW - Tumor Cells, Cultured KW - Down-Regulation KW - Sp1 Transcription Factor -- metabolism KW - Colonic Neoplasms -- metabolism KW - Colonic Neoplasms -- pathology KW - Transcription Factors -- metabolism KW - Chromosomal Instability KW - Proto-Oncogene Proteins -- metabolism KW - DNA-Binding Proteins -- genetics KW - Transcription, Genetic KW - Promoter Regions, Genetic -- genetics KW - Proto-Oncogene Proteins -- genetics KW - Cell Hypoxia KW - Transcription Factors -- genetics KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67533941?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cell&rft.atitle=HIF-1alpha+induces+genetic+instability+by+transcriptionally+downregulating+MutSalpha+expression.&rft.au=Koshiji%2C+Minori%3BTo%2C+Kenneth+K-W%3BHammer%2C+Stefanie%3BKumamoto%2C+Kensuke%3BHarris%2C+Adrian+L%3BModrich%2C+Paul%3BHuang%2C+L+Eric&rft.aulast=Koshiji&rft.aufirst=Minori&rft.date=2005-03-18&rft.volume=17&rft.issue=6&rft.spage=793&rft.isbn=&rft.btitle=&rft.title=Molecular+cell&rft.issn=10972765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-26 N1 - Date created - 2005-03-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cancer. An anchor for tumor cell invasion. AN - 67523054; 15774745 JF - Science (New York, N.Y.) AU - Yuspa, Stuart H AU - Epstein, Ervin H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. sy12j@nih.gov Y1 - 2005/03/18/ PY - 2005 DA - 2005 Mar 18 SP - 1727 EP - 1728 VL - 307 IS - 5716 KW - Cell Adhesion Molecules KW - 0 KW - Collagen Type VII KW - I-kappa B Proteins KW - NFKBIA protein, human KW - Nfkbia protein, mouse KW - Transforming Growth Factor beta KW - kalinin KW - NF-KappaB Inhibitor alpha KW - 139874-52-5 KW - Index Medicus KW - Animals KW - Neoplasm Invasiveness KW - Disease Susceptibility KW - Humans KW - I-kappa B Proteins -- metabolism KW - Transduction, Genetic KW - Mice KW - Genes, ras KW - Cell Adhesion Molecules -- metabolism KW - I-kappa B Proteins -- genetics KW - Protein Structure, Tertiary KW - Transforming Growth Factor beta -- metabolism KW - Mutation KW - Cell Transformation, Neoplastic KW - Epidermolysis Bullosa Dystrophica -- metabolism KW - Carcinoma, Squamous Cell -- etiology KW - Collagen Type VII -- chemistry KW - Skin Neoplasms -- etiology KW - Skin Neoplasms -- pathology KW - Collagen Type VII -- genetics KW - Epidermolysis Bullosa Dystrophica -- complications KW - Epidermolysis Bullosa Dystrophica -- pathology KW - Skin Neoplasms -- genetics KW - Skin Neoplasms -- physiopathology KW - Epidermolysis Bullosa Dystrophica -- genetics KW - Carcinoma, Squamous Cell -- pathology KW - Carcinoma, Squamous Cell -- physiopathology KW - Carcinoma, Squamous Cell -- genetics KW - Keratinocytes -- pathology KW - Keratinocytes -- metabolism KW - Collagen Type VII -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67523054?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Science+%28New+York%2C+N.Y.%29&rft.atitle=Cancer.+An+anchor+for+tumor+cell+invasion.&rft.au=Yuspa%2C+Stuart+H%3BEpstein%2C+Ervin+H&rft.aulast=Yuspa&rft.aufirst=Stuart&rft.date=2005-03-18&rft.volume=307&rft.issue=5716&rft.spage=1727&rft.isbn=&rft.btitle=&rft.title=Science+%28New+York%2C+N.Y.%29&rft.issn=1095-9203&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-31 N1 - Date created - 2005-03-18 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment On: Science. 2005 Mar 18;307(5716):1773-6 [15774758] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Purified Bacillus anthracis Lethal Toxin Complex Formed in Vitro and during Infection Exhibits Functional and Biological Activity AN - 17482917; 6189550 AB - Anthrax protective antigen (PA, 83 kDa), a pore-forming protein, upon protease activation to 63 kDa (PA sub(63)), translocates lethal factor (LF) and edema factor (EF) from endosomes into the cytosol of the cell. The relatively small size of the heptameric PA sub(63) pore ([approx]12 Aa) raises questions as to how large molecules such as LF and EF can move through the pore. In addition, the reported high binding affinity between PA and EF/LF suggests that EF/LF may not dissociate but remain complexed with activated PA sub(63). In this study, we found that purified (PA sub(63)) sub(7)-LF complex exhibited biological and functional activities similar to the free LF. Purified LF complexed with PA sub(63) heptamer was able to cleave both a synthetic peptide substrate and endogenous mitogen-activated protein kinase kinase substrates and kill susceptible macrophage cells. Electrophysiological studies of the complex showed strong rectification of the ionic current at positive voltages, an effect similar to that observed if LF is added to the channels formed by heptameric PA sub(63) pore. Complexes of (PA sub(63)) sub(7)-LF found in the plasma of infected animals showed functional activity. Identifying active complex in the blood of infected animals has important implications for therapeutic design, especially those directed against PA and LF. Our studies suggest that the individual toxin components and the complex must be considered as critical targets for anthrax therapeutics. JF - Journal of Biological Chemistry AU - Panchal, Rekha G AU - Halverson, Kelly M AU - Ribot, Wilson AU - Lane, Douglas AU - Kenny, Tara AU - Abshire, Teresa G AU - Ezzell, John W AU - Hoover, Timothy A AU - Powell, Bradford AU - Little, Stephen AU - Kasianowicz, John J AU - Bavari, Sina AD - Developmental Therapeutics Program, Target Structure-based Drug Discovery Group, NCI SAIC-Frederick, National Institutes of Health, Frederick, Maryland Y1 - 2005/03/18/ PY - 2005 DA - 2005 Mar 18 SP - 10834 EP - 10839 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 11 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Macrophages KW - MAP kinase KW - synthetic peptides KW - Channel pores KW - Lethal factor KW - protective antigen KW - Edema KW - pore-forming proteins KW - Bacillus anthracis KW - Toxins KW - endosomes KW - Anthrax KW - Proteinase KW - J 02823:In vitro and in vivo effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17482917?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Purified+Bacillus+anthracis+Lethal+Toxin+Complex+Formed+in+Vitro+and+during+Infection+Exhibits+Functional+and+Biological+Activity&rft.au=Panchal%2C+Rekha+G%3BHalverson%2C+Kelly+M%3BRibot%2C+Wilson%3BLane%2C+Douglas%3BKenny%2C+Tara%3BAbshire%2C+Teresa+G%3BEzzell%2C+John+W%3BHoover%2C+Timothy+A%3BPowell%2C+Bradford%3BLittle%2C+Stephen%3BKasianowicz%2C+John+J%3BBavari%2C+Sina&rft.aulast=Panchal&rft.aufirst=Rekha&rft.date=2005-03-18&rft.volume=280&rft.issue=11&rft.spage=10834&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Bacillus anthracis; Channel pores; Toxins; Anthrax; Proteinase; protective antigen; MAP kinase; synthetic peptides; Macrophages; Edema; pore-forming proteins; endosomes; Lethal factor ER - TY - JOUR T1 - Deriving the glutamate clearance time course from transporter currents in CA1 hippocampal astrocytes: transmitter uptake gets faster during development. AN - 67527890; 15772350 AB - At many excitatory synapses, the neurotransmitter glutamate diffuses beyond the synaptic cleft to activate extrasynaptic targets. The extent and impact of such transmitter "spillover" on the processing capacity of neuronal networks are unclear, in part because it remains unknown how far transmitter diffuses from its point of release before being removed from the extracellular space by high-affinity glutamate transporters. Synaptically activated, transporter-mediated currents (STCs) recorded in hippocampal astrocytes provide an experimental measure of glutamate uptake, but the time course of the STC may be shaped, or "filtered," by other factors and therefore not represent a direct indication of clearance rate. Here, STCs were recorded from astrocytes in rat hippocampal slices under conditions in which uptake capacity was reduced and the STC decay reflected a slowed rate of glutamate clearance. The temporal characteristics of the filtering mechanisms were extracted from these responses, and the glutamate clearance time course in control conditions was derived. The results indicate that glutamate can be cleared from the extrasynaptic space within 1 ms. Clearance is fastest in adult neuropil, corresponding to a developmental increase in glial transporter expression. Synaptically released glutamate is taken up at the same rate as glutamate released via flash photolysis, indicating that the spatial location of transporters relative to the site of glutamate release does not affect the time course of clearance. Slower clearance in young animals would permit glutamate to diffuse greater distances, indicating a particularly important role for extrasynaptic receptors early in development. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Diamond, Jeffrey S AD - Synaptic Physiology Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-3701, USA. diamondj@ninds.nih.gov Y1 - 2005/03/16/ PY - 2005 DA - 2005 Mar 16 SP - 2906 EP - 2916 VL - 25 IS - 11 KW - Amino Acid Transport System X-AG KW - 0 KW - Excitatory Amino Acid Antagonists KW - Glutamates KW - alpha-(4,5-dimethoxy-2-nitrobenzyl) glutamate KW - benzyloxyaspartate KW - Picrotoxin KW - 124-87-8 KW - Aspartic Acid KW - 30KYC7MIAI KW - Glutamic Acid KW - 3KX376GY7L KW - picrotoxinin KW - 9K011NUF0R KW - Index Medicus KW - Patch-Clamp Techniques -- methods KW - Animals KW - Membrane Potentials -- radiation effects KW - Age Factors KW - Electric Stimulation -- methods KW - Dose-Response Relationship, Drug KW - Dose-Response Relationship, Radiation KW - Excitatory Amino Acid Antagonists -- pharmacology KW - Rats KW - Animals, Newborn KW - Aspartic Acid -- pharmacology KW - Rats, Sprague-Dawley KW - Excitatory Postsynaptic Potentials -- drug effects KW - Glutamates -- pharmacology KW - Excitatory Postsynaptic Potentials -- radiation effects KW - Picrotoxin -- pharmacology KW - In Vitro Techniques KW - Picrotoxin -- analogs & derivatives KW - Amino Acid Transport System X-AG -- antagonists & inhibitors KW - Time Factors KW - Male KW - Excitatory Postsynaptic Potentials -- physiology KW - Synapses -- physiology KW - Glutamic Acid -- metabolism KW - Synapses -- drug effects KW - Hippocampus -- growth & development KW - Synapses -- radiation effects KW - Hippocampus -- cytology KW - Astrocytes -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67527890?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Deriving+the+glutamate+clearance+time+course+from+transporter+currents+in+CA1+hippocampal+astrocytes%3A+transmitter+uptake+gets+faster+during+development.&rft.au=Diamond%2C+Jeffrey+S&rft.aulast=Diamond&rft.aufirst=Jeffrey&rft.date=2005-03-16&rft.volume=25&rft.issue=11&rft.spage=2906&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-13 N1 - Date created - 2005-03-17 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: J Neurosci. 2005 Apr 6;25(14):3739 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad. AN - 67493969; 15751984 AB - Deregulation of PI3K/Akt and Raf/Mek/Erk signal transduction cascades is one of the principal causes of neoplastic transformation. The inactivation of the proapoptotic protein Bad, upon phosphorylation by different kinases of these two pathways, may play an important role in different human malignancies. Therefore, we have expressed and purified a new chimeric protein, hGM-CSF-Bad, linking the human granulocyte-macrophage colony-stimulating factor to the N-terminus of the proapoptotic protein human Bad, to deliver Bad into tumor cells and induce apoptosis. Indeed, the human GM-CSF receptor is a good target because it is overexpressed on many leukemias and solid tumors and is not detectable on stem cells. We found that the chimeric protein binds the human GM-CSF receptor, is endocytosed, and appears to reach the cytosol via retrograde ER transport. After entering cells, the protein is able to induce apoptosis of human leukemia cells and human colon and gastric carcinoma cell lines (IC(50) values as low as 1 muM). We conclude that GM-CSF-Bad can overcome the inappropriate survival stimuli in transformed cells and restore the apoptotic pathway. The completely human sequence and the elevated selectivity for cancer cells could prevent immunogenicity and the nonspecific toxicity of targeted toxins in future clinical application of this fusion protein. JF - Biochemistry AU - Antignani, Antonella AU - Youle, Richard J AD - Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-3704, USA. Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 4074 EP - 4082 VL - 44 IS - 10 SN - 0006-2960, 0006-2960 KW - BAD protein, human KW - 0 KW - BH3 Interacting Domain Death Agonist Protein KW - BID protein, human KW - Carrier Proteins KW - Growth Inhibitors KW - Proto-Oncogene Proteins c-bcl-2 KW - Receptors, Granulocyte-Macrophage Colony-Stimulating Factor KW - Recombinant Fusion Proteins KW - bcl-Associated Death Protein KW - Serine KW - 452VLY9402 KW - Granulocyte-Macrophage Colony-Stimulating Factor KW - 83869-56-1 KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Cell Survival -- genetics KW - HL-60 Cells KW - HeLa Cells KW - Humans KW - Granulocyte-Macrophage Colony-Stimulating Factor -- toxicity KW - Growth Inhibitors -- genetics KW - Intracellular Fluid -- metabolism KW - Cell Line, Tumor KW - Serine -- genetics KW - Growth Inhibitors -- toxicity KW - Protein Structure, Tertiary -- genetics KW - Receptors, Granulocyte-Macrophage Colony-Stimulating Factor -- metabolism KW - Protein Transport -- genetics KW - Phosphorylation KW - Granulocyte-Macrophage Colony-Stimulating Factor -- genetics KW - Receptors, Granulocyte-Macrophage Colony-Stimulating Factor -- genetics KW - Alanine -- genetics KW - Granulocyte-Macrophage Colony-Stimulating Factor -- metabolism KW - U937 Cells KW - Growth Inhibitors -- metabolism KW - Recombinant Fusion Proteins -- metabolism KW - Apoptosis -- genetics KW - Carrier Proteins -- metabolism KW - Carrier Proteins -- genetics KW - Proto-Oncogene Proteins c-bcl-2 -- metabolism KW - Carrier Proteins -- toxicity KW - Carrier Proteins -- physiology KW - Recombinant Fusion Proteins -- toxicity KW - Proto-Oncogene Proteins c-bcl-2 -- genetics KW - Recombinant Fusion Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67493969?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=A+chimeric+protein+induces+tumor+cell+apoptosis+by+delivering+the+human+Bcl-2+family+BH3-only+protein+Bad.&rft.au=Antignani%2C+Antonella%3BYoule%2C+Richard+J&rft.aulast=Antignani&rft.aufirst=Antonella&rft.date=2005-03-15&rft.volume=44&rft.issue=10&rft.spage=4074&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-03-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Biomarkers of oxidative stress study II: are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning? AN - 67450110; 15721980 AB - Oxidation products of lipids, proteins, and DNA in the blood, plasma, and urine of rats were measured as part of a comprehensive, multilaboratory validation study searching for noninvasive biomarkers of oxidative stress. This article is the second report of the nationwide Biomarkers of Oxidative Stress Study using acute CCl4 poisoning as a rodent model for oxidative stress. The time-dependent (2, 7, and 16 h) and dose-dependent (120 and 1200 mg/kg i.p.) effects of CCl4 on concentrations of lipid hydroperoxides, TBARS, malondialdehyde (MDA), isoprostanes, protein carbonyls, methionine sulfoxidation, tyrosine products, 8-hydroxy-2'-deoxyguanosine (8-OHdG), leukocyte DNA-MDA adducts, and DNA-strand breaks were investigated to determine whether the oxidative effects of CCl4 would result in increased generation of these oxidation products. Plasma concentrations of MDA and isoprostanes (both measured by GC-MS) and urinary concentrations of isoprostanes (measured with an immunoassay or LC/MS/MS) were increased in both low-dose and high-dose CCl4-treated rats at more than one time point. The other urinary markers (MDA and 8-OHdG) showed significant elevations with treatment under three of the four conditions tested. It is concluded that measurements of MDA and isoprostanes in plasma and urine as well as 8-OHdG in urine are potential candidates for general biomarkers of oxidative stress. All other products were not changed by CCl4 or showed fewer significant effects. JF - Free radical biology & medicine AU - Kadiiska, M B AU - Gladen, B C AU - Baird, D D AU - Germolec, D AU - Graham, L B AU - Parker, C E AU - Nyska, A AU - Wachsman, J T AU - Ames, B N AU - Basu, S AU - Brot, N AU - Fitzgerald, G A AU - Floyd, R A AU - George, M AU - Heinecke, J W AU - Hatch, G E AU - Hensley, K AU - Lawson, J A AU - Marnett, L J AU - Morrow, J D AU - Murray, D M AU - Plastaras, J AU - Roberts, L J AU - Rokach, J AU - Shigenaga, M K AU - Sohal, R S AU - Sun, J AU - Tice, R R AU - Van Thiel, D H AU - Wellner, D AU - Walter, P B AU - Tomer, K B AU - Mason, R P AU - Barrett, J C AD - Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, P.O. Box 12233, MD F0-02, Research Triangle Park, NC 27709, USA. Kadiiska@niehs.nih.gov Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 698 EP - 710 VL - 38 IS - 6 SN - 0891-5849, 0891-5849 KW - Free Radicals KW - 0 KW - Thiobarbituric Acid Reactive Substances KW - Tyrosine KW - 42HK56048U KW - Malondialdehyde KW - 4Y8F71G49Q KW - 8-oxo-7-hydrodeoxyguanosine KW - 88847-89-6 KW - DNA KW - 9007-49-2 KW - Methionine KW - AE28F7PNPL KW - Hydrogen Peroxide KW - BBX060AN9V KW - Carbon Tetrachloride KW - CL2T97X0V0 KW - Deoxyguanosine KW - G9481N71RO KW - Oxygen KW - S88TT14065 KW - Index Medicus KW - Animals KW - Comet Assay KW - Immunoblotting KW - DNA Damage KW - Oxygen -- metabolism KW - Hydrogen Peroxide -- metabolism KW - Methionine -- metabolism KW - Liver -- metabolism KW - Tyrosine -- chemistry KW - Rats KW - Rats, Inbred F344 KW - Malondialdehyde -- pharmacology KW - Gas Chromatography-Mass Spectrometry KW - Spectrophotometry KW - Tyrosine -- metabolism KW - Time Factors KW - Male KW - Immunoassay KW - Carbon Tetrachloride Poisoning -- metabolism KW - DNA -- metabolism KW - Oxidative Stress KW - Deoxyguanosine -- pharmacology KW - Carbon Tetrachloride -- toxicity KW - Lipid Metabolism KW - Deoxyguanosine -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67450110?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Biomarkers+of+oxidative+stress+study+II%3A+are+oxidation+products+of+lipids%2C+proteins%2C+and+DNA+markers+of+CCl4+poisoning%3F&rft.au=Kadiiska%2C+M+B%3BGladen%2C+B+C%3BBaird%2C+D+D%3BGermolec%2C+D%3BGraham%2C+L+B%3BParker%2C+C+E%3BNyska%2C+A%3BWachsman%2C+J+T%3BAmes%2C+B+N%3BBasu%2C+S%3BBrot%2C+N%3BFitzgerald%2C+G+A%3BFloyd%2C+R+A%3BGeorge%2C+M%3BHeinecke%2C+J+W%3BHatch%2C+G+E%3BHensley%2C+K%3BLawson%2C+J+A%3BMarnett%2C+L+J%3BMorrow%2C+J+D%3BMurray%2C+D+M%3BPlastaras%2C+J%3BRoberts%2C+L+J%3BRokach%2C+J%3BShigenaga%2C+M+K%3BSohal%2C+R+S%3BSun%2C+J%3BTice%2C+R+R%3BVan+Thiel%2C+D+H%3BWellner%2C+D%3BWalter%2C+P+B%3BTomer%2C+K+B%3BMason%2C+R+P%3BBarrett%2C+J+C&rft.aulast=Kadiiska&rft.aufirst=M&rft.date=2005-03-15&rft.volume=38&rft.issue=6&rft.spage=698&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-02-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Biomarkers of oxidative stress study III. Effects of the nonsteroidal anti-inflammatory agents indomethacin and meclofenamic acid on measurements of oxidative products of lipids in CCl4 poisoning. AN - 67445853; 15721981 AB - Plasma and urinary levels of malondialdehyde-like products (MDA) and isoprostanes were identified as markers of in vivo lipid peroxidation in an animal model of CCl4 poisoning. We sought to determine the extent to which the formation of these oxidation products is influenced by inhibition of the cyclooxygenase enzymes which catalytically generate proinflammatory lipid peroxidation products known as prostaglandins and thromboxane. In the present studies, after induction of oxidant stress in rats with CCl4, lipid peroxidation products measured in plasma and urine demonstrate that isoprostanes and MDA can be partially inhibited by cyclooxygenase inhibitors, albeit to different extents. The lowering of isoprostane and MDA formation, however, may not to due primarily to the diminution of catalytic generation of isoprostanes or MDA by the cyclooxygenases but, rather, may be the result of the suppression of nonenzymatic lipid peroxidation. This is suggested since 8,12-iso-iPF2alpha-VI is also reduced by indomethacin, yet, unlike other isoprostanes and MDA, it is not generated catalytically by the cyclooxygenase. Thus, although the two cyclooxygenase inhibitors we tested have statistically significant effects on the measurements of both isoprostanes and MDA in this study, the results provide evidence that these lipid-degradation products primarily constitute markers of oxidative stress. JF - Free radical biology & medicine AU - Kadiiska, M B AU - Gladen, B C AU - Baird, D D AU - Graham, L B AU - Parker, C E AU - Ames, B N AU - Basu, S AU - Fitzgerald, G A AU - Lawson, J A AU - Marnett, L J AU - Morrow, J D AU - Murray, D M AU - Plastaras, J AU - Roberts, L J AU - Rokach, J AU - Shigenaga, M K AU - Sun, J AU - Walter, P B AU - Tomer, K B AU - Barrett, J C AU - Mason, R P AD - National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Kadiiska@niehs.nih.gov Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 711 EP - 718 VL - 38 IS - 6 SN - 0891-5849, 0891-5849 KW - Anti-Inflammatory Agents, Non-Steroidal KW - 0 KW - Biomarkers KW - Free Radicals KW - Prostaglandins KW - Protein Isoforms KW - Meclofenamic Acid KW - 48I5LU4ZWD KW - Thromboxane A2 KW - 57576-52-0 KW - Carbon Tetrachloride KW - CL2T97X0V0 KW - Oxygen KW - S88TT14065 KW - Indomethacin KW - XXE1CET956 KW - Index Medicus KW - Animals KW - Mass Spectrometry KW - Oxygen -- metabolism KW - Lipid Peroxidation KW - Chromatography, High Pressure Liquid KW - Inflammation KW - Rats KW - Rats, Inbred F344 KW - Thromboxane A2 -- metabolism KW - Gas Chromatography-Mass Spectrometry KW - Time Factors KW - Prostaglandins -- metabolism KW - Immunoassay KW - Meclofenamic Acid -- pharmacology KW - Indomethacin -- metabolism KW - Oxidative Stress KW - Carbon Tetrachloride Poisoning -- drug therapy KW - Biomarkers -- metabolism KW - Carbon Tetrachloride -- toxicity KW - Lipid Metabolism KW - Indomethacin -- pharmacology KW - Anti-Inflammatory Agents, Non-Steroidal -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67445853?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Regions+of+the+varicella-zoster+virus+open+reading+frame+63+latency-associated+protein+important+for+replication+in+vitro+are+also+critical+for+efficient+establishment+of+latency.&rft.au=Cohen%2C+Jeffrey+I%3BKrogmann%2C+Tammy%3BBontems%2C+Sebastien%3BSadzot-Delvaux%2C+Catherine%3BPesnicak%2C+Lesley&rft.aulast=Cohen&rft.aufirst=Jeffrey&rft.date=2005-04-01&rft.volume=79&rft.issue=8&rft.spage=5069&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-02-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitric oxide and chemically induced hepatotoxicity: beneficial effects of the liver-selective nitric oxide donor, V-PYRRO/NO. AN - 67401840; 15691592 AB - Nitric oxide (NO) is endogenously produced by the enzyme NO synthase in the cell or pharmacologically delivered to tissues as NO prodrugs. This simple molecule is a potent biological mediator in a myriad of physiological and pathological events. The liver plays a central role in metabolism and immune processes, and is a major target organ influenced by NO. NO production in the liver is usually increased in response to acute insult with hepatotoxicants, and may be decreased during chronic liver diseases. The induction of NO production could be envisioned as an early adaptive response, which may become a mediator of tissue damage when in excess. In this regard, inhibition of endogenous NO synthase has been shown to be beneficial in some cases and detrimental in others. The creation of eNOS and iNOS knockout animals has advanced our understanding of NO function in hepatic response to toxic insults. Knocking endogenous NO production can be beneficial in response to certain toxicants; however, in general it weakens the body's defense mechanisms against toxic insults. A variety of pharmacological NO prodrugs have been developed, and, when used appropriately, most of them have demonstrated beneficial effects in the liver in a variety of pathological settings. In this review, we discuss the relationship between NO and hepatotoxicity, and the beneficial effects of NO donors on the liver, using the liver-selective NO donor, V-PYRRO/NO, as an example to demonstrate that pharmacologically delivered NO could have therapeutic benefits for liver disorders. JF - Toxicology AU - Liu, Jie AU - Waalkes, Michael P AD - Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at NIEHS, Mail Drop F0-09, Research Triangle Park, NC 27709, USA. liu6@niehs.nih.gov Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 289 EP - 297 VL - 208 IS - 2 SN - 0300-483X, 0300-483X KW - Nitric Oxide Donors KW - 0 KW - O(2)-vinyl-1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate KW - Pyrrolidines KW - Xenobiotics KW - Nitric Oxide KW - 31C4KY9ESH KW - Index Medicus KW - Animals KW - Liver -- drug effects KW - Humans KW - Liver -- metabolism KW - Nitric Oxide Donors -- pharmacology KW - Chemical and Drug Induced Liver Injury -- etiology KW - Pyrrolidines -- therapeutic use KW - Nitric Oxide Donors -- therapeutic use KW - Drug-Related Side Effects and Adverse Reactions KW - Xenobiotics -- adverse effects KW - Pyrrolidines -- pharmacology KW - Nitric Oxide -- biosynthesis KW - Chemical and Drug Induced Liver Injury -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67401840?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=Nitric+oxide+and+chemically+induced+hepatotoxicity%3A+beneficial+effects+of+the+liver-selective+nitric+oxide+donor%2C+V-PYRRO%2FNO.&rft.au=Liu%2C+Jie%3BWaalkes%2C+Michael+P&rft.aulast=Liu&rft.aufirst=Jie&rft.date=2005-03-15&rft.volume=208&rft.issue=2&rft.spage=289&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-15 N1 - Date created - 2005-02-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparison of phenethyl and 6-phenylhexyl isothiocyanate-induced toxicity in rat esophageal cell lines with and without glutathione depletion. AN - 67361627; 15649627 AB - Phenethyl isothiocyanate (PEITC) and its synthetic homolog, 6-phenylhexyl isothiocyanate (PHITC), are both potent inhibitors of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung mice tumorigenesis. However, unlike PEITC, PHITC enhanced N-nitrosomethylbenzylamine (NMBA)-induced rat esophageal tumorigenesis. These findings imply that due to its unique chemical properties, PHITC's effects on esophageal cells are procarcinogenic rather than chemopreventive. Relative to PEITC, PHITC is more lipophilic and less reactive, which could result in higher PHITC intracellular levels. Due to ITCs' inherently high level of thiol reactivity, increased intracellular levels of PHITC have the potential to deplete intracellular glutathione (GSH) reserves. Since GSH is a primary intracellular antioxidant and cytoprotective enzyme cofactor, preservation of intracellular GSH status is crucial for cytoprotection. Despite the recognized importance of isothiocyanate structure with the potential for toxicity, no studies have yet investigated the association between the primary intracellular free thiol, GSH, and isothiocyanate-induced toxicity in this target cell population. The present study investigated whether PEITC and PHITC display unique cytotoxic profiles in cultured rat esophageal cells, and also monitored the effects of ITC challenge on cellular GSH status. A final series of experiments investigated the converse i.e., affects of modulation of intracellular GSH status on ITC-mediated toxicity. Dose-response curves revealed that PEITC was significantly more toxic in tumorigenic and non-tumorigenic cells relative to PHITC. The ITC-GSH interaction studies demonstrated comparable GSH levels following either PEITC or PHITC challenge, and also showed that GSH depletion did not augment ITC-mediated cellular toxicity. While our data demonstrate structure related differences in ITC-mediated cytotoxicities, these differences do not appear to be directly attributable to cellular GSH pools. JF - Toxicology letters AU - Hudson, Tamaro S AU - Stoner, Gary D AU - Morse, Mark A AU - Young, Heather AU - Mallery, Susan R AD - Division of Cancer Prevention, Cancer Prevention Fellowship Program, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA. Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 427 EP - 436 VL - 155 IS - 3 SN - 0378-4274, 0378-4274 KW - Carcinogens KW - 0 KW - Isoenzymes KW - Isothiocyanates KW - 6-phenylhexyl isothiocyanate KW - 133920-06-6 KW - phenethyl isothiocyanate KW - 6U7TFK75KV KW - Glutathione Peroxidase KW - EC 1.11.1.9 KW - Glutathione Reductase KW - EC 1.8.1.7 KW - Hypoxanthine Phosphoribosyltransferase KW - EC 2.4.2.8 KW - Glutathione Transferase KW - EC 2.5.1.18 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Rats KW - Animals KW - Glutathione Peroxidase -- metabolism KW - Glutathione Reductase -- metabolism KW - Glutathione Transferase -- metabolism KW - Hypoxanthine Phosphoribosyltransferase -- metabolism KW - Isoenzymes -- metabolism KW - Cell Line KW - Cell Survival KW - Isothiocyanates -- toxicity KW - Glutathione -- metabolism KW - Carcinogens -- toxicity KW - Glutathione -- deficiency UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67361627?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+letters&rft.atitle=Comparison+of+phenethyl+and+6-phenylhexyl+isothiocyanate-induced+toxicity+in+rat+esophageal+cell+lines+with+and+without+glutathione+depletion.&rft.au=Hudson%2C+Tamaro+S%3BStoner%2C+Gary+D%3BMorse%2C+Mark+A%3BYoung%2C+Heather%3BMallery%2C+Susan+R&rft.aulast=Hudson&rft.aufirst=Tamaro&rft.date=2005-03-15&rft.volume=155&rft.issue=3&rft.spage=427&rft.isbn=&rft.btitle=&rft.title=Toxicology+letters&rft.issn=03784274&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-04 N1 - Date created - 2005-01-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Advances in MR elastic displacement imaging and non-invasive measurements of myocardial compliance AN - 40040348; 3924611 AU - Wen, H Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 7000:Multidisciplinary UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40040348?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Advances+in+MR+elastic+displacement+imaging+and+non-invasive+measurements+of+myocardial+compliance&rft.au=Wen%2C+H&rft.aulast=Wen&rft.aufirst=H&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: AVS Science & Technology Society, ; phone: 212 248 0200; fax: 212 248 0245; URL: www.avs.org/ N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Ternary SNARE complexes are associated with lipid rafts in RBL mast cells AN - 40022528; 3915301 AU - Puri, N AU - Roche, P A Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40022528?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Ternary+SNARE+complexes+are+associated+with+lipid+rafts+in+RBL+mast+cells&rft.au=Puri%2C+N%3BRoche%2C+P+A&rft.aulast=Puri&rft.aufirst=N&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Functional architecture of the ATP-dependent Clp proteases: Discrimination of static and mobile elements by cryo-electron microscopy AN - 40017228; 3915455 AU - Ishikawa, T AU - Maurizi, M R AU - Steven, A C Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40017228?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lancet+%28London%2C+England%29&rft.atitle=Hormones+and+endometrial+cancer--new+data+from+the+Million+Women+Study.&rft.au=Brinton%2C+Louise+A%3BLacey%2C+James+V%3BTrimble%2C+Edward+L&rft.aulast=Brinton&rft.aufirst=Louise&rft.date=2005-04-01&rft.volume=365&rft.issue=9470&rft.spage=1517&rft.isbn=&rft.btitle=&rft.title=Lancet+%28London%2C+England%29&rft.issn=1474-547X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Evidence for endoplasmic reticulum origins of peroxisomes AN - 40014351; 3915506 AU - Kim, P K AU - Mullen, R T AU - Lippincott-Schwartz, J Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40014351?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Evidence+for+endoplasmic+reticulum+origins+of+peroxisomes&rft.au=Kim%2C+P+K%3BMullen%2C+R+T%3BLippincott-Schwartz%2C+J&rft.aulast=Kim&rft.aufirst=P&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Depletion of GAK/Auxilin 2 by RNAi: Effect on clathrin distribution and receptor mediated endocytosis AN - 39998705; 3915318 AU - Lee, D AU - Zhao, X AU - Greene, L AU - Eisenberg, E Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39998705?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Depletion+of+GAK%2FAuxilin+2+by+RNAi%3A+Effect+on+clathrin+distribution+and+receptor+mediated+endocytosis&rft.au=Lee%2C+D%3BZhao%2C+X%3BGreene%2C+L%3BEisenberg%2C+E&rft.aulast=Lee&rft.aufirst=D&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Quantitative analysis of microtubule polymerization in extracellular matrix-mediated axon guidance AN - 39968629; 3915174 AU - Vartanian, K AU - Katagiri, Y AU - Peter, P AU - Tan, F AU - Wang, H AU - Geller, H M Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39968629?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Quantitative+analysis+of+microtubule+polymerization+in+extracellular+matrix-mediated+axon+guidance&rft.au=Vartanian%2C+K%3BKatagiri%2C+Y%3BPeter%2C+P%3BTan%2C+F%3BWang%2C+H%3BGeller%2C+H+M&rft.aulast=Vartanian&rft.aufirst=K&rft.date=2005-03-15&rft.volume=&rft.issue=4&rft.spage=401&rft.isbn=&rft.btitle=&rft.title=Medical+care&rft.issn=00257079&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Fusion pathway mediated by sindbis virus E1 glycoprotein: Membrane intermediates and capsid-envelope interactions AN - 39966181; 3915287 AU - Zaitseva, E AU - Mittal, A AU - Griffin, D AU - Chernomordik, L Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39966181?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Fusion+pathway+mediated+by+sindbis+virus+E1+glycoprotein%3A+Membrane+intermediates+and+capsid-envelope+interactions&rft.au=Zaitseva%2C+E%3BMittal%2C+A%3BGriffin%2C+D%3BChernomordik%2C+L&rft.aulast=Zaitseva&rft.aufirst=E&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Retrocyclin-2 inhibits early stages of membrane fusion reactions mediated by envelope glycoproteins of diverse viruses AN - 39953975; 3915288 AU - Leikina, E AU - Melikov, K AU - Delanoe, H AU - Waring, A J AU - Lehrer, R I AU - Chernomordik, LV Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39953975?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Retrocyclin-2+inhibits+early+stages+of+membrane+fusion+reactions+mediated+by+envelope+glycoproteins+of+diverse+viruses&rft.au=Leikina%2C+E%3BMelikov%2C+K%3BDelanoe%2C+H%3BWaring%2C+A+J%3BLehrer%2C+R+I%3BChernomordik%2C+LV&rft.aulast=Leikina&rft.aufirst=E&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - T cells' signaling machinery sets the threshold for sensitive and fast self/nonself discrimination AN - 39925920; 3915495 AU - Altan-Bonnet, G AU - Stefanova, I AU - Germain, R N Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39925920?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=T+cells%27+signaling+machinery+sets+the+threshold+for+sensitive+and+fast+self%2Fnonself+discrimination&rft.au=Altan-Bonnet%2C+G%3BStefanova%2C+I%3BGermain%2C+R+N&rft.aulast=Altan-Bonnet&rft.aufirst=G&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Characterization of early stages of nuclear envelope assembly driven by cytosolic proteins AN - 39902450; 3915337 AU - Ramos, C AU - Melikov, K C AU - Zaitseva, E AU - Wilson, K L AU - Chernomordik, LV Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39902450?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+therapeutics&rft.atitle=Inhibition+of+the+epidermal+growth+factor+receptor+increases+expression+of+genes+that+stimulate+inflammation%2C+apoptosis%2C+and+cell+attachment.&rft.au=Woodworth%2C+Craig+D%3BMichael%2C+Evan%3BMarker%2C+Dan%3BAllen%2C+Sarah%3BSmith%2C+Laura%3BNees%2C+Matthias&rft.aulast=Woodworth&rft.aufirst=Craig&rft.date=2005-04-01&rft.volume=4&rft.issue=4&rft.spage=650&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - High-throughput antibody screening of clinical serum samples AN - 39896989; 3915449 AU - Gannot, G AU - Tangrea, MA AU - Gillespie, J W AU - Erickson, H S AU - Wallis, B S AU - Knezevic, V AU - Hartmann, D P AU - Chuaqui, R F AU - Emmert-Buck, M R Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39896989?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=High-throughput+antibody+screening+of+clinical+serum+samples&rft.au=Gannot%2C+G%3BTangrea%2C+MA%3BGillespie%2C+J+W%3BErickson%2C+H+S%3BWallis%2C+B+S%3BKnezevic%2C+V%3BHartmann%2C+D+P%3BChuaqui%2C+R+F%3BEmmert-Buck%2C+M+R&rft.aulast=Gannot&rft.aufirst=G&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Isoform specific glycosylation of tau by O-linked GlcNAc transferase: Implications for tauopathies AN - 39892797; 3914976 AU - Lazarus, B D AU - Hanover, JA Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39892797?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Isoform+specific+glycosylation+of+tau+by+O-linked+GlcNAc+transferase%3A+Implications+for+tauopathies&rft.au=Lazarus%2C+B+D%3BHanover%2C+JA&rft.aulast=Lazarus&rft.aufirst=B&rft.date=2005-03-15&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American Society for Cell Biology, 8120 Woodmont Avenue, Suite 750, Bethesda, MD 20814-2762, USA; email: ascbinfo@ascb.org; URL: www.ascb.org N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver AN - 17288716; 6173442 AB - Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the risk of rAAV integration because integration occurs preferentially in genes and accompanies chromosomal deletions, which may lead to loss-of-function insertional mutagenesis. Here, by analyzing 347 rAAV integrations in mice, we elucidate novel features of rAAV integration: the presence of hot spots for integration and a strong preference for integrating near gene regulatory sequences. The most prominent hot spot was a harmless chromosomal niche in the rRNA gene repeats, whereas nearly half of the integrations landed near transcription start sites or CpG islands, suggesting the possibility of activating flanking cellular disease genes by vector integration, similar to retroviral gain-of-function insertional mutagenesis. Possible cancer-related genes were hit by rAAV integration at a frequency of 3.5%. In addition, the information about chromosomal changes at 218 integration sites and 602 breakpoints of vector genomes have provided a clue to how vector terminal repeats and host chromosomal DNA are joined in the integration process. Thus, the present study provides new insights into the risk of rAAV-mediated insertional mutagenesis and the mechanisms of rAAV integration. JF - Journal of Virology AU - Nakai, Hiroyuki AU - Wu, Xiaolin AU - Fuess, Sally AU - Storm, Theresa A AU - Munroe, David AU - Montini, Eugenio AU - Burgess, Shawn M AU - Grompe, Markus AU - Kay, Mark A AD - Departments of Pediatrics. Genetics, Stanford University School of Medicine, Stanford, California. Laboratory of Molecular Technology, SAIC-Frederick, National Cancer Institute-Frederick, Frederick. Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland. Departments of Molecular and Medical Genetics. Pediatrics, Oregon Health & Science University, Portland, Oregon Y1 - 2005/03/15/ PY - 2005 DA - 2005 Mar 15 SP - 3606 EP - 3614 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 6 SN - 0022-538X, 0022-538X KW - mice KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts KW - Genomes KW - Hot spots KW - Adeno-associated virus KW - Expression vectors KW - Breakpoints KW - rRNA KW - Integration KW - insertional mutagenesis KW - Gene therapy KW - Regulatory sequences KW - Transcription KW - CpG islands KW - Chromosome deletion KW - Liver KW - W3 33181:Gene therapy vectors KW - V 22050:Viral genetics including virus reactivation KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17288716?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Large-Scale+Molecular+Characterization+of+Adeno-Associated+Virus+Vector+Integration+in+Mouse+Liver&rft.au=Nakai%2C+Hiroyuki%3BWu%2C+Xiaolin%3BFuess%2C+Sally%3BStorm%2C+Theresa+A%3BMunroe%2C+David%3BMontini%2C+Eugenio%3BBurgess%2C+Shawn+M%3BGrompe%2C+Markus%3BKay%2C+Mark+A&rft.aulast=Nakai&rft.aufirst=Hiroyuki&rft.date=2005-03-15&rft.volume=79&rft.issue=6&rft.spage=3606&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adeno-associated virus; Integration; insertional mutagenesis; Hot spots; Genomes; CpG islands; rRNA; Chromosome deletion; Regulatory sequences; Transcription; Expression vectors; Liver; Breakpoints; Gene therapy ER - TY - JOUR T1 - Thiazide diuretics and the risk of gallbladder disease requiring surgery in women. AN - 67515179; 15767534 AB - Previous studies have suggested that thiazide diuretic use increases the risk of cholecystitis. We prospectively examined the association between thiazide use and cholecystectomy, a surrogate for symptomatic cholelithiasis, in a cohort of 81 351 US women who were aged 30 to 55 years in 1980 and followed up to 2000. Regular use of thiazide diuretics was assessed at baseline by asking the participants to report whether they currently took "any of the following medications in most weeks" and listing "thiazide diuretics (eg, Diuril and Hydrodiuril)" among other drugs. Respondents were also requested to report the duration of thiazide diuretic use. Assessment of thiazide diuretic use was updated in 1982, 1988, 1994, 1996, and 1998. Cox regression was used to adjust simultaneously for other potential risk factors for cholecystectomy. During follow-up, 8607 women reported undergoing a cholecystectomy. A modest positive relation between the use of thiazide diuretics and cholecystectomy was observed. Compared with never users of thiazide diuretics, the multivariate relative risk of cholecystectomy for past users was 1.16 (95% confidence interval,1.08-1.24) and the multivariate relative risk for current users was 1.39 (95% confidence interval, 1.29-1.50). These findings are compatible with the hypothesis that the use of thiazide diuretics increases the risk of symptomatic cholecystitis. However, we cannot rule out the possibility that our results are in part explained by unconsidered factors related to the indication for antihypertensive therapy or by differences in medical surveillance between users and nonusers of thiazide diuretics. JF - Archives of internal medicine AU - Leitzmann, Michael F AU - Tsai, Chung-Jyi AU - Stampfer, Meir J AU - Willett, Walter C AU - Giovannucci, Edward AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, EPS-MSC 7232, 6120 Executive Boulevard, Bethesda, MD 20892, USA. leitzmann@mail.nih.gov Y1 - 2005/03/14/ PY - 2005 DA - 2005 Mar 14 SP - 567 EP - 573 VL - 165 IS - 5 SN - 0003-9926, 0003-9926 KW - Benzothiadiazines KW - 0 KW - Diuretics KW - Sodium Chloride Symporter Inhibitors KW - Abridged Index Medicus KW - Index Medicus KW - Risk KW - Prospective Studies KW - Humans KW - Cohort Studies KW - Adult KW - Incidence KW - Middle Aged KW - Cholecystectomy -- statistics & numerical data KW - Time Factors KW - Female KW - Proportional Hazards Models KW - Multivariate Analysis KW - Cholecystitis -- chemically induced KW - Sodium Chloride Symporter Inhibitors -- adverse effects KW - Cholecystitis -- surgery UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67515179?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+internal+medicine&rft.atitle=Thiazide+diuretics+and+the+risk+of+gallbladder+disease+requiring+surgery+in+women.&rft.au=Leitzmann%2C+Michael+F%3BTsai%2C+Chung-Jyi%3BStampfer%2C+Meir+J%3BWillett%2C+Walter+C%3BGiovannucci%2C+Edward&rft.aulast=Leitzmann&rft.aufirst=Michael&rft.date=2005-03-14&rft.volume=165&rft.issue=5&rft.spage=567&rft.isbn=&rft.btitle=&rft.title=Archives+of+internal+medicine&rft.issn=00039926&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effect of nandrolone decanoate therapy on weight and lean body mass in HIV-infected women with weight loss: a randomized, double-blind, placebo-controlled, multicenter trial. AN - 67513524; 15767536 AB - Weight loss is associated with accelerated mortality and disease progression in patients with human immunodeficiency virus (HIV) infection. Although studies have examined a variety of anabolic therapies in HIV-infected men, the safety and efficacy of such treatments in women have not been adequately studied. In this randomized, double-blind, placebo-controlled, multicenter, phase I/II study, 38 HIV-infected women with documented weight loss of 5% or greater in the preceding year or a body mass index of less than 20 kg/m(2) were randomized to receive nandrolone decanoate (100 mg) or an equivalent volume of placebo every other week by intramuscular injection. Subjects received blinded treatment for 12 weeks, followed by open-label therapy for 12 weeks. Lean body mass and fat (bioelectrical impedance analysis) and weight were measured at baseline and at weeks 6, 12, 18, and 24. Biochemical assessments of safety (hematologic analyses, liver function tests, and sex hormone measurements) were performed at these same time points. Clinical signs and symptoms were monitored biweekly. Subjects randomized to receive nandrolone had significant increases in weight and lean body mass during blinded treatment (4.6 kg [9.0%] and 3.5 kg [8.6%], respectively; P<.001 vs baseline and placebo in each case). Fat mass did not change statistically significantly in either group. Although there were no statistically significant differences between groups in biochemical measures, the number of grade 3 or greater toxicities, or reports of virilizing effects, a full assessment of safety cannot be made in a trial of this size. Nandrolone decanoate therapy may prove to be generally safe and beneficial in reversing weight loss and lean tissue loss in women with HIV infection and other chronic catabolic diseases. JF - Archives of internal medicine AU - Mulligan, Kathleen AU - Zackin, Robert AU - Clark, Rebecca A AU - Alston-Smith, Beverly AU - Liu, Tun AU - Sattler, Fred R AU - Delvers, Thomas B AU - Currier, Judith S AU - AIDS Clinical Trials Group 329 Study Team AU - National Institute of Allergy and Infectious Diseases Adult AIDS Clinical Trials Group AD - Division of Endocrinology, San Francisco General Hospital, 1001 Potrero Avenue, Rm. 3501K, San Francisco, CA 94110, USA. kmulligan@sfghgcrc.ucsf.edu ; AIDS Clinical Trials Group 329 Study Team ; National Institute of Allergy and Infectious Diseases Adult AIDS Clinical Trials Group Y1 - 2005/03/14/ PY - 2005 DA - 2005 Mar 14 SP - 578 EP - 585 VL - 165 IS - 5 SN - 0003-9926, 0003-9926 KW - Anabolic Agents KW - 0 KW - Hormones KW - Nandrolone KW - 6PG9VR430D KW - nandrolone decanoate KW - H45187T098 KW - Abridged Index Medicus KW - Index Medicus KW - Nutritional Status KW - Blood Chemical Analysis KW - Double-Blind Method KW - Humans KW - Safety KW - Injections, Intramuscular KW - Hormones -- blood KW - Body Mass Index KW - Hematologic Tests KW - Placebo Effect KW - Adult KW - Body Weight -- drug effects KW - Treatment Outcome KW - Female KW - HIV Wasting Syndrome -- blood KW - HIV Wasting Syndrome -- drug therapy KW - Anabolic Agents -- therapeutic use KW - Nandrolone -- analogs & derivatives KW - Nandrolone -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67513524?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+internal+medicine&rft.atitle=Effect+of+nandrolone+decanoate+therapy+on+weight+and+lean+body+mass+in+HIV-infected+women+with+weight+loss%3A+a+randomized%2C+double-blind%2C+placebo-controlled%2C+multicenter+trial.&rft.au=Mulligan%2C+Kathleen%3BZackin%2C+Robert%3BClark%2C+Rebecca+A%3BAlston-Smith%2C+Beverly%3BLiu%2C+Tun%3BSattler%2C+Fred+R%3BDelvers%2C+Thomas+B%3BCurrier%2C+Judith+S%3BAIDS+Clinical+Trials+Group+329+Study+Team%3BNational+Institute+of+Allergy+and+Infectious+Diseases+Adult+AIDS+Clinical+Trials+Group&rft.aulast=Mulligan&rft.aufirst=Kathleen&rft.date=2005-04-01&rft.volume=30&rft.issue=4&rft.spage=720&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Metal-catalyzed oxidation of alpha-synuclein: helping to define the relationship between oligomers, protofibrils, and filaments. AN - 67487475; 15615715 AB - Oxidative stress is implicated in a number of neuro-degenerative diseases and is associated with the selective loss of dopaminergic neurons of the substantia nigra in Parkinson's disease. The role of alpha-synuclein as a potential target of intracellular oxidants has been demonstrated by the identification of posttranslational modifications of synuclein within intracellular aggregates that accumulate in Parkinson's disease brains, as well as the ability of a number of oxidative insults to induce synuclein oligomerization. The relationship between these relatively small soluble oligomers, potentially neurotoxic synuclein protofibrils, and synuclein filaments remains unclear. We have found that metal-catalyzed oxidation of alpha-synuclein inhibited formation of synuclein filaments with a concomitant accumulation of beta sheet-rich oligomers that may represent synuclein protofibrils. Similar results with a number of oxidative and enzymatic treatments suggest that the covalent association of synuclein into higher molecular mass oligomers/protofibrils represents an alternate pathway from filament formation and renders synuclein less prone to proteasomal degradation. JF - The Journal of biological chemistry AU - Cole, Nelson B AU - Murphy, Diane D AU - Lebowitz, Jacob AU - Di Noto, Luca AU - Levine, Rodney L AU - Nussbaum, Robert L AD - Genetic Diseases Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. ncole@mail.nih.gov Y1 - 2005/03/11/ PY - 2005 DA - 2005 Mar 11 SP - 9678 EP - 9690 VL - 280 IS - 10 SN - 0021-9258, 0021-9258 KW - Macromolecular Substances KW - 0 KW - Metals KW - Nerve Tissue Proteins KW - Oxidants KW - Recombinant Proteins KW - SNCA protein, human KW - Synucleins KW - alpha-Synuclein KW - Iron KW - E1UOL152H7 KW - Index Medicus KW - Actin Cytoskeleton -- ultrastructure KW - Humans KW - Protein Processing, Post-Translational KW - Parkinson Disease -- metabolism KW - Brain -- metabolism KW - Macromolecular Substances -- metabolism KW - Models, Biological KW - Iron -- metabolism KW - Oxidation-Reduction KW - Recombinant Proteins -- metabolism KW - Kinetics KW - Oxidative Stress KW - Oxidants -- metabolism KW - Recombinant Proteins -- chemistry KW - Macromolecular Substances -- chemistry KW - Nerve Tissue Proteins -- metabolism KW - Metals -- metabolism KW - Nerve Tissue Proteins -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67487475?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Metal-catalyzed+oxidation+of+alpha-synuclein%3A+helping+to+define+the+relationship+between+oligomers%2C+protofibrils%2C+and+filaments.&rft.au=Cole%2C+Nelson+B%3BMurphy%2C+Diane+D%3BLebowitz%2C+Jacob%3BDi+Noto%2C+Luca%3BLevine%2C+Rodney+L%3BNussbaum%2C+Robert+L&rft.aulast=Cole&rft.aufirst=Nelson&rft.date=2005-03-11&rft.volume=280&rft.issue=10&rft.spage=9678&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-19 N1 - Date created - 2005-03-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The Salmonella Effector Protein SopB Protects Epithelial Cells from Apoptosis by Sustained Activation of Akt AN - 17617880; 6189892 AB - Invasion of epithelial cells by Salmonella enterica is mediated by bacterial "effector" proteins that are delivered into the host cell by a type III secretion system. Although primarily known for their roles in actin rearrangements and membrane ruffling, translocated effectors also affect host cell processes that are not directly associated with invasion. Here, we show that SopB/SigD, an effector with phosphoinositide phosphatase activity, has anti-apoptotic activity in Salmonella-infected epithelial cells. Salmonella induced the sustained activation of Akt/protein kinase B, a pro-survival kinase, in a SopB-dependent manner. Failure to activate Akt resulted in increased levels of apoptosis after infection with a sopB deletion mutant ( delta sopB). Furthermore, cells infected with wild type bacteria, but not the delta sopB strain, were protected from camptothecin-induced cleavage of caspase-3 and subsequent apoptosis. The anti-apoptotic activity of SopB was dependent on its phosphatase activity, because a catalytically inactive mutant was unable to protect cells from the effects of camptothecin. Finally, small interfering RNA was used to demonstrate the essential role of Akt in SopB-mediated protection against apoptosis. These results provide new insights into the mechanisms of apoptosis and highlight how bacterial effectors can intercept signaling pathways to manipulate host responses. JF - Journal of Biological Chemistry AU - Knodler, Leigh A AU - Finlay, BBrett AU - Steele-Mortimer, Olivia AD - Laboratory of Intracellular Parasites, NIAID, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana Y1 - 2005/03/11/ PY - 2005 DA - 2005 Mar 11 SP - 9058 EP - 9064 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 10 SN - 0021-9258, 0021-9258 KW - SigD protein KW - SopB protein KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - G 07320:Bacterial genetics KW - J 02727:Amino acids, peptides and proteins UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17617880?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=The+Salmonella+Effector+Protein+SopB+Protects+Epithelial+Cells+from+Apoptosis+by+Sustained+Activation+of+Akt&rft.au=Knodler%2C+Leigh+A%3BFinlay%2C+BBrett%3BSteele-Mortimer%2C+Olivia&rft.aulast=Knodler&rft.aufirst=Leigh&rft.date=2005-03-11&rft.volume=280&rft.issue=10&rft.spage=9058&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-07-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - A receptor domain controls the intracellular sorting of the ferrichrome transporter, ARN1. AN - 67504115; 15719020 AB - The Saccharomyces cerevisiae transporter Arn1p takes up the ferric-siderophore ferrichrome, and extracellular ferrichrome dramatically influences the intracellular trafficking of Arn1p. In the absence of ferrichrome, Arn1p sorts directly to the endosomal compartment. At low concentrations of ferrichrome, Arn1p stably relocalizes to the plasma membrane, yet little to no uptake of ferrichrome occurs at these low concentrations. At higher concentrations of ferrichrome, Arn1p cycles between the plasma membrane and endosome. Arn1p contains two binding sites for ferrichrome: one site has an affinity similar to the K(T) for transport, but the second site has a much higher affinity. Here we report that this high-affinity binding site lies within a unique extracytosolic, carboxyl-terminal domain. Mutations within this domain lead to loss of ferrichrome binding and uptake activities and missorting of Arn1p, including a failure to relocalize to the plasma membrane in the presence of ferrichrome. Mutation of phenylalanine residues in the cytosolic tail of Arn1p also lead to missorting, but without defects in ferrichrome binding. We propose that the carboxyl terminus of Arn1p contains a receptor domain that controls the intracellular trafficking of the transporter. JF - The EMBO journal AU - Kim, Youngwoo AU - Lampert, Sarah M AU - Philpott, Caroline C AD - Liver Diseases Branch, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/03/09/ PY - 2005 DA - 2005 Mar 09 SP - 952 EP - 962 VL - 24 IS - 5 SN - 0261-4189, 0261-4189 KW - Arn1 protein, S cerevisiae KW - 0 KW - DNA, Fungal KW - Membrane Transport Proteins KW - Saccharomyces cerevisiae Proteins KW - Ubiquitin KW - Ferrichrome KW - 15630-64-5 KW - Alanine KW - OF5P57N2ZX KW - Index Medicus KW - Cytosol -- metabolism KW - Models, Molecular KW - Alanine -- chemistry KW - Biological Transport, Active KW - Models, Biological KW - Mutagenesis, Site-Directed KW - Saccharomyces cerevisiae -- genetics KW - Base Sequence KW - Ubiquitin -- metabolism KW - Ubiquitin -- chemistry KW - Saccharomyces cerevisiae -- metabolism KW - DNA, Fungal -- genetics KW - Binding Sites -- genetics KW - Cell Membrane -- metabolism KW - Protein Structure, Tertiary KW - Saccharomyces cerevisiae Proteins -- metabolism KW - Saccharomyces cerevisiae Proteins -- genetics KW - Membrane Transport Proteins -- chemistry KW - Ferrichrome -- metabolism KW - Saccharomyces cerevisiae Proteins -- chemistry KW - Membrane Transport Proteins -- metabolism KW - Membrane Transport Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67504115?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+EMBO+journal&rft.atitle=A+receptor+domain+controls+the+intracellular+sorting+of+the+ferrichrome+transporter%2C+ARN1.&rft.au=Kim%2C+Youngwoo%3BLampert%2C+Sarah+M%3BPhilpott%2C+Caroline+C&rft.aulast=Kim&rft.aufirst=Youngwoo&rft.date=2005-03-09&rft.volume=24&rft.issue=5&rft.spage=952&rft.isbn=&rft.btitle=&rft.title=The+EMBO+journal&rft.issn=02614189&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-28 N1 - Date created - 2005-03-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2003 May 13;100(10):5664-9 [12721368] EMBO J. 2003 May 15;22(10):2309-17 [12743025] Science. 2003 Aug 1;301(5633):616-20 [12893936] FEBS Lett. 2003 Nov 27;555(1):96-101 [14630326] Nat Rev Mol Cell Biol. 2004 Jan;5(1):23-32 [14708007] Mol Biol Cell. 2004 Feb;15(2):883-95 [14657252] J Gen Microbiol. 1987 Nov;133(11):3229-36 [3328775] Genetics. 1989 May;122(1):19-27 [2659436] Mol Cell Biol. 1990 May;10(5):2294-301 [2183029] J Biol Chem. 1991 Nov 5;266(31):21150-7 [1718971] Cell. 1994 Jan 28;76(2):403-10 [8293473] Mol Cell Biol. 1994 May;14(5):3065-73 [8164662] J Biol Chem. 1995 Nov 10;270(45):26723-6 [7592901] Science. 1996 Mar 15;271(5255):1552-7 [8599111] EMBO J. 1996 Nov 15;15(22):6084-95 [8947031] Yeast. 1997 Jan;13(1):43-54 [9046086] J Biol Chem. 1997 Aug 22;272(34):21461-6 [9261163] EMBO J. 1998 Sep 1;17(17):5026-36 [9724638] Science. 1998 Dec 18;282(5397):2215-20 [9856937] Nat Struct Biol. 2000 Apr;7(4):287-91 [10742172] Biochem J. 2000 Mar 1;346 Pt 2:329-36 [10677350] Microbiology. 1998 Dec;144 ( Pt 12):3455-62 [9884238] J Biol Chem. 2000 Apr 7;275(14):10709-15 [10744769] FEMS Microbiol Lett. 2000 May 15;186(2):221-7 [10802175] Biometals. 1999 Dec;12(4):301-6 [10816729] J Biol Chem. 2000 May 26;275(21):16354-9 [10748025] Biometals. 2000 Mar;13(1):65-72 [10831226] Mol Biol Cell. 2001 Feb;12(2):421-35 [11179425] Nat Rev Mol Cell Biol. 2001 Mar;2(3):195-201 [11265249] J Biol Chem. 2001 Mar 30;276(13):10218-23 [11120744] Curr Biol. 2001 Nov 13;11(22):R932-4 [11719242] Mol Biol Cell. 2001 Dec;12(12):4129-38 [11739806] EMBO J. 2002 Jul 15;21(14):3632-42 [12110576] Traffic. 2002 Aug;3(8):537-46 [12121417] J Biol Chem. 2002 Aug 23;277(34):30598-605 [12060662] Biochim Biophys Acta. 2003 Feb 17;1610(1):11-22 [12586375] Science. 2003 Aug 1;301(5633):610-5 [12893935] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I. AN - 67470063; 15736931 AB - Beta-1,4-galactosyltransferase I (beta4Gal-T1) normally transfers Gal from UDP-Gal to GlcNAc in the presence of Mn(2+) ion (Gal-T activity) and also transfers Glc from UDP-Glc to GlcNAc (Glc-T activity), albeit at only 0.3% efficiency. In addition, alpha-lactalbumin (LA) enhances this Glc-T activity more than 25 times. Comparison of the crystal structures of UDP-Gal- and UDP-Glc-bound beta4Gal-T1 reveals that the O4 hydroxyl group in both Gal and Glc moieties forms a hydrogen bond with the side chain carboxylate group of Glu317. The orientation of the O4 hydroxyl of glucose causes a steric hindrance to the side chain carboxylate group of Glu317, accounting for the enzyme's low Glc-T activity. In this study, we show that mutation of Arg228, a residue in the vicinity of Glu317, to lysine (R228K-Gal-T1) results in a 15-fold higher Glc-T activity, which is further enhanced by LA to nearly 25% of the Gal-T activity of the wild type. The kinetic parameters indicate that the main effect of the mutation of Arg228 to lysine is on the k(cat) of Glc-T, which increases 3-4-fold, both in the absence and in the presence of LA; simultaneously, the k(cat) for the Gal-T reaction is reduced 30-fold. The crystal structure of R228K-Gal-T1 complexed with LA, UDP-Gal, and Mn(2+) determined at 1.9 A resolution shows that the Asp318 side chain exhibits a minor alternate conformation, compared to that in the wild type. This alternate conformation now causes a steric hindrance to the O4 hydroxyl group of the Gal moiety of UDP-Gal, probably causing the dissociation of UDP-Gal and the reduced k(cat) of the Gal-T reaction. JF - Biochemistry AU - Ramakrishnan, Boopathy AU - Boeggeman, Elizabeth AU - Qasba, Pradman K AD - Structural Glycobiology Section, Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, Maryland 21702-1201, USA. Y1 - 2005/03/08/ PY - 2005 DA - 2005 Mar 08 SP - 3202 EP - 3210 VL - 44 IS - 9 SN - 0006-2960, 0006-2960 KW - Macromolecular Substances KW - 0 KW - Uridine Diphosphate Galactose KW - 2956-16-3 KW - Manganese KW - 42Z2K6ZL8P KW - Lactalbumin KW - 9013-90-5 KW - Arginine KW - 94ZLA3W45F KW - Galactosyltransferases KW - EC 2.4.1.- KW - Glucosyltransferases KW - beta-1,4-galactosyltransferase I KW - Lysine KW - K3Z4F929H6 KW - Uridine Diphosphate Glucose KW - V50K1D7P4Y KW - Index Medicus KW - Crystallization KW - Animals KW - Lactalbumin -- metabolism KW - Cattle KW - Kinetics KW - Manganese -- chemistry KW - Uridine Diphosphate Galactose -- metabolism KW - Glucosyltransferases -- metabolism KW - Crystallography, X-Ray KW - Macromolecular Substances -- metabolism KW - Uridine Diphosphate Glucose -- metabolism KW - Enzyme Activation -- genetics KW - Mutagenesis, Site-Directed KW - Galactosyltransferases -- metabolism KW - Arginine -- genetics KW - Galactosyltransferases -- genetics KW - Lysine -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67470063?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Mutation+of+arginine+228+to+lysine+enhances+the+glucosyltransferase+activity+of+bovine+beta-1%2C4-galactosyltransferase+I.&rft.au=Ramakrishnan%2C+Boopathy%3BBoeggeman%2C+Elizabeth%3BQasba%2C+Pradman+K&rft.aulast=Ramakrishnan&rft.aufirst=Boopathy&rft.date=2005-03-08&rft.volume=44&rft.issue=9&rft.spage=3202&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-06 N1 - Date created - 2005-03-01 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1YRO; PDB N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - (salen)MnIII compounds as nonpeptidyl mimics of catalase. Mechanism-based tuning of catalase activity: a theoretical study. AN - 67468366; 15732983 AB - We present the results of the first theoretical investigation of salen-manganese complexes as synthetic catalytic scavengers of hydrogen peroxide molecules that mimic catalase enzymes. Catalase mimics can be used as therapeutic agents against oxidative stress in treatment of many diseases, including Alzheimer's disease, stroke, heart disease, aging, and cancer. A ping-pong mechanism approach has been considered to describe the H2O2 dismutation reaction. The real compounds reacting with a peroxide molecule were utilized in our BP density functional calculations to avoid uncertainties connected with using incomplete models. Part I of the dismutation reaction-converting a peroxide molecule into a water molecule with simultaneous oxidation of the metal atom of the catalyst-can be done quite effectively at the Mn catalytic center. To act as catalytic scavengers of hydrogen peroxide, the oxomanganese salen complexes have to be deoxidized during part II of the dismutation reaction. It has been shown that there are two possible reaction routes for the second part of the dismutation reaction: the top and the side substrate approach routes. Our results suggest that the catalyst could be at least temporarily deactivated (poisoned) in the side approach reaction route due to the formation of a kinetically stable intermediate. Overall, the side approach reaction route for the catalyst recovery is the bottleneck for the whole dismutation process. On the basis of the detailed knowledge of the mode of action of the (salen)MnIII catalase mimics, we suggest and rationalize structural changes of the catalyst that should lead to better therapeutic properties. The available experimental data support our conclusions. Our findings on the reaction dismutation mechanism could be the starting point for further improvement of salen-manganese complexes as synthetic catalytic scavengers of reactive oxygen species. JF - Inorganic chemistry AU - Abashkin, Yuri G AU - Burt, Stanley K AD - Advanced Biomedical Computing Center, SAIC-Frederick, Inc., National Cancer Institute at Frederick, PO Box B, Frederick, Maryland 21702-1201, USA. abashkin@ncifcrf.gov Y1 - 2005/03/07/ PY - 2005 DA - 2005 Mar 07 SP - 1425 EP - 1432 VL - 44 IS - 5 SN - 0020-1669, 0020-1669 KW - Ethylenediamines KW - 0 KW - Organometallic Compounds KW - N,N'-bis(salicylideneamino)ethane-manganese(II) KW - 53177-12-1 KW - Hydrogen Peroxide KW - BBX060AN9V KW - Catalase KW - EC 1.11.1.6 KW - Index Medicus KW - Molecular Structure KW - Models, Molecular KW - Kinetics KW - Molecular Mimicry KW - Models, Chemical KW - Molecular Conformation KW - Catalase -- metabolism KW - Catalase -- chemistry KW - Ethylenediamines -- chemistry KW - Organometallic Compounds -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67468366?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Inorganic+chemistry&rft.atitle=%28salen%29MnIII+compounds+as+nonpeptidyl+mimics+of+catalase.+Mechanism-based+tuning+of+catalase+activity%3A+a+theoretical+study.&rft.au=Abashkin%2C+Yuri+G%3BBurt%2C+Stanley+K&rft.aulast=Abashkin&rft.aufirst=Yuri&rft.date=2005-03-07&rft.volume=44&rft.issue=5&rft.spage=1425&rft.isbn=&rft.btitle=&rft.title=Inorganic+chemistry&rft.issn=00201669&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-17 N1 - Date created - 2005-02-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Three-dimensional Structure of a Macromolecular Assembly that Regulates Type III Secretion in Yersinia pestis AN - 20816645; 8247600 AB - Yersinia pestis, the causative agent of plague, utilizes a type III secretion system (T3SS) to inject effector proteins directly into the cytosol of mammalian cells where they interfere with signal transduction pathways that regulate actin cytoskeleton dynamics and inflammation, thereby enabling the bacterium to avoid engulfment and destruction by macrophages. Type III secretion normally does not occur in the absence of close contact with eukaryotic cells. Negative regulation is mediated in part by a multiprotein complex that has been proposed to act as a physical impediment to type III secretion by blocking the entrance to the secretion apparatus prior to contact with mammalian cells. This complex is composed of YopN, its heterodimeric secretion chaperone SycN-YscB, and TyeA. Here, we report two crystal structures of YopN in complex with its heterodimeric secretion chaperone SycN-YscB and the co-regulatory protein TyeA, respectively. By merging these two overlapping structures, it was possible to construct a credible theoretical model of the YopN-SycN-YscB-TyeA complex. The modeled assembly features the secretion signaling elements of YopN at one end of an elongated structure and the secretion regulating TyeA binding site at the other. A patch of highly conserved residues on the surface of the C-terminal alpha -helix of TyeA may mediate its interaction with structural components of the secretion apparatus. Conserved arginine residues that reside inside a prominent cavity at the dimer interface of SycN-YscB were mutated in order to investigate whether they play a role in targeting the YopN-chaperone complex to the type III secretion apparatus. One of the mutants exhibited a phenotype that is consistent with this hypothesis. JF - Journal of Molecular Biology AU - Schubot, F D AU - Jackson, M W AU - Penrose, K J AU - Cherry, S AU - Tropea, JE AU - Plano, G V AU - Waugh, D S AD - Center for Cancer Research, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702-1201, USA, waughd@ncifcrf.gov Y1 - 2005/03/04/ PY - 2005 DA - 2005 Mar 04 SP - 1147 EP - 1161 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 346 IS - 4 SN - 0022-2836, 0022-2836 KW - Microbiology Abstracts B: Bacteriology KW - Macrophages KW - Macromolecules KW - Arginine KW - Secretion KW - Yersinia pestis KW - Inflammation KW - Cytoskeleton KW - Mammalian cells KW - Crystal structure KW - Cytosol KW - Actin KW - Chaperones KW - Plague KW - Signal transduction KW - J 02310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20816645?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Molecular+Biology&rft.atitle=Three-dimensional+Structure+of+a+Macromolecular+Assembly+that+Regulates+Type+III+Secretion+in+Yersinia+pestis&rft.au=Schubot%2C+F+D%3BJackson%2C+M+W%3BPenrose%2C+K+J%3BCherry%2C+S%3BTropea%2C+JE%3BPlano%2C+G+V%3BWaugh%2C+D+S&rft.aulast=Schubot&rft.aufirst=F&rft.date=2005-03-04&rft.volume=346&rft.issue=4&rft.spage=1147&rft.isbn=&rft.btitle=&rft.title=Journal+of+Molecular+Biology&rft.issn=00222836&rft_id=info:doi/10.1016%2Fj.jmb.2004.12.036 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Macrophages; Macromolecules; Arginine; Secretion; Inflammation; Cytoskeleton; Mammalian cells; Cytosol; Crystal structure; Chaperones; Actin; Plague; Signal transduction; Yersinia pestis DO - http://dx.doi.org/10.1016/j.jmb.2004.12.036 ER - TY - JOUR T1 - Risks and benefits of phase 1 oncology trials, 1991 through 2002. AN - 67484075; 15745980 AB - Previous reviews of phase 1 oncology trials reported a rate of response to treatment of 4 to 6 percent and a toxicity-related death rate of 0.5 percent. These results may not reflect the rates in current phase 1 oncology trials. We reviewed all nonpediatric phase 1 oncology trials sponsored by the Cancer Therapy Evaluation Program at the National Cancer Institute between 1991 and 2002. We report the rates of response to treatment, of stable disease, of grade 4 toxic events, and of treatment-related deaths. We analyzed 460 trials involving 11,935 participants, all of whom were assessed for toxicity and 10,402 of whom were assessed for a response to therapy. The overall response rate (i.e., for both complete and partial responses) was 10.6 percent, with considerable variation among trials. "Classic" phase 1 trials of single investigational chemotherapeutic agents represented only 20 percent of the trials and had a response rate of 4.4 percent. Studies that included at least one anticancer agent approved by the Food and Drug Administration constituted 46.3 percent of the trials and had a response rate of 17.8. An additional 34.1 percent of participants had stable disease or a less-than-partial response. The overall rate of death due to toxic events was 0.49 percent. Of 3465 participants for whom data on patient-specific grade 4 toxic events were available, 14.3 percent had had at least one episode of grade 4 toxic events. Overall response rates among phase 1 oncology trials are higher than previously reported, although they have not changed for classic phase 1 trials, and toxicity-related death rates have remained stable. Rates of response and toxicity vary, however, among the various types of phase 1 oncology trials. Copyright 2005 Massachusetts Medical Society. JF - The New England journal of medicine AU - Horstmann, Elizabeth AU - McCabe, Mary S AU - Grochow, Louise AU - Yamamoto, Seiichiro AU - Rubinstein, Larry AU - Budd, Troy AU - Shoemaker, Dale AU - Emanuel, Ezekiel J AU - Grady, Christine AD - Department of Clinical Bioethics, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Md 20892-1156, USA. Y1 - 2005/03/03/ PY - 2005 DA - 2005 Mar 03 SP - 895 EP - 904 VL - 352 IS - 9 KW - Antineoplastic Agents KW - 0 KW - Cancer Vaccines KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Retrospective Studies KW - Outcome Assessment (Health Care) KW - Risk Assessment KW - Genetic Therapy -- adverse effects KW - Cancer Vaccines -- adverse effects KW - Neoplasms -- mortality KW - Clinical Trials, Phase I as Topic KW - Cancer Vaccines -- therapeutic use KW - Neoplasms -- therapy KW - Antineoplastic Agents -- therapeutic use KW - Antineoplastic Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67484075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Risks+and+benefits+of+phase+1+oncology+trials%2C+1991+through+2002.&rft.au=Horstmann%2C+Elizabeth%3BMcCabe%2C+Mary+S%3BGrochow%2C+Louise%3BYamamoto%2C+Seiichiro%3BRubinstein%2C+Larry%3BBudd%2C+Troy%3BShoemaker%2C+Dale%3BEmanuel%2C+Ezekiel+J%3BGrady%2C+Christine&rft.aulast=Horstmann&rft.aufirst=Elizabeth&rft.date=2005-03-03&rft.volume=352&rft.issue=9&rft.spage=895&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=1533-4406&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-08 N1 - Date created - 2005-03-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: N Engl J Med. 2005 Mar 3;352(9):930-2 [15745986] N Engl J Med. 2005 Jun 9;352(23):2451-3; author reply 2451-3 [15948271] N Engl J Med. 2005 Jun 9;352(23):2451-3; author reply 2451-3 [15948269] N Engl J Med. 2005 Jun 9;352(23):2451-3; author reply 2451-3 [15944433] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tumor suppressor p53 represses transcription of RECQ4 helicase. AN - 67479548; 15674334 AB - RECQ4 is a member of the RecQ helicase family, which has been implicated in the regulation of DNA replication, recombination and repair. p53 modulates the functions of RecQ helicases including BLM and WRN. In this study, we demonstrate that p53 can regulate the transcription of RECQ4. Using nontransformed, immortalized normal human fibroblasts, we show that p53-dependent downregulation of RECQ4 expression occurred in G1-arrested cells, both in the absence or presence of exogenous DNA damage. Wild-type p53 (but not the tumor-derived mutant forms) repressed RECQ4 promoter activity. The camptothecin or etoposide-dependent p53-mediated repression was attenuated by trichostatin A (TSA), an inhibitor of histone deacetylases (HDACs). Repression of the RECQ4 promoter was accompanied with an increased accumulation of HDAC1, and the loss of SP1 and p53 binding to the promoter. The simultaneous formation of a camptothecin-dependent p53-SP1 complex indicated its occurrence outside of the RECQ4 promoter. These data suggest that p53-mediated repression of RECQ4 transcription during DNA damage results from the modulation of the promoter occupancy of transcription activators and repressors. JF - Oncogene AU - Sengupta, Sagar AU - Shimamoto, Akira AU - Koshiji, Minori AU - Pedeux, Remy AU - Rusin, Marek AU - Spillare, Elisa A AU - Shen, Jiang Cheng AU - Huang, L Eric AU - Lindor, Noralane M AU - Furuichi, Yasuhiro AU - Harris, Curtis C AD - Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/03/03/ PY - 2005 DA - 2005 Mar 03 SP - 1738 EP - 1748 VL - 24 IS - 10 SN - 0950-9232, 0950-9232 KW - Hydroxamic Acids KW - 0 KW - Repressor Proteins KW - Tumor Suppressor Protein p53 KW - trichostatin A KW - 3X2S926L3Z KW - Histone Deacetylases KW - EC 3.5.1.98 KW - DNA Helicases KW - EC 3.6.4.- KW - RecQ Helicases KW - EC 3.6.4.12 KW - Index Medicus KW - Promoter Regions, Genetic KW - DNA Damage KW - Cells, Cultured KW - Humans KW - Histone Deacetylases -- metabolism KW - G1 Phase KW - Hydroxamic Acids -- pharmacology KW - Transcriptional Activation KW - Tumor Suppressor Protein p53 -- physiology KW - Repressor Proteins -- physiology KW - DNA Helicases -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67479548?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oncogene&rft.atitle=Tumor+suppressor+p53+represses+transcription+of+RECQ4+helicase.&rft.au=Sengupta%2C+Sagar%3BShimamoto%2C+Akira%3BKoshiji%2C+Minori%3BPedeux%2C+Remy%3BRusin%2C+Marek%3BSpillare%2C+Elisa+A%3BShen%2C+Jiang+Cheng%3BHuang%2C+L+Eric%3BLindor%2C+Noralane+M%3BFuruichi%2C+Yasuhiro%3BHarris%2C+Curtis+C&rft.aulast=Sengupta&rft.aufirst=Sagar&rft.date=2005-03-03&rft.volume=24&rft.issue=10&rft.spage=1738&rft.isbn=&rft.btitle=&rft.title=Oncogene&rft.issn=09509232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-24 N1 - Date created - 2005-03-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serum alpha-Tocopherol and gamma-Tocopherol in Relation to Prostate Cancer Risk in a Prospective Study AN - 20713221; 6190845 AB - The Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study demonstrated a 32% reduction in prostate cancer incidence in response to daily alpha-tocopherol supplementation. We examined baseline serum concentrations of alpha-tocopherol and gamma-tocopherol to compare their respective associations with prostate cancer risk. From the ATBC Study cohort of 29 133 Finnish men, 50- 69 years old, we randomly selected 100 incident prostate cancer case patients and matched 200 control subjects. Odds ratios and 95% confidence intervals (CIs) were estimated for the serum tocopherols (measured by high-performance liquid chromatography) using logistic regression models. All P values were two-sided. Odds ratios for the highest versus the lowest tertiles were 0.49 (95% CI = 0.24 to 1.01, P sub(trend) = .05) for alpha-tocopherol and 0.57 (95% CI = 0.31 to 1.06, P sub(trend) = .08) for gamma-tocopherol. Further analyses indicated that the association of high serum tocopherols with low prostate cancer risk was stronger in the alpha-tocopherol-supplemented group than in those not receiving alpha- tocopherol. Participants with higher circulating concentrations of the major vitamin E fractions, alpha-tocopherol and gamma-tocopherol, had similarly lower prostate cancer risk. JF - Journal of the National Cancer Institute AU - Weinstein, Stephanie J AU - Wright, Margaret E AU - Pietinen, Pirjo AU - King, Irena AU - Tan, Carly AU - Taylor, Philip R AU - Virtamo, Jarmo AU - Albanes, Demetrius AD - Division of Cancer Epidemiology and Genetics (SJW, MEW, DA) and Center for Cancer Research (PRT), National Cancer Institute, NIH, DHHS, Bethesda, MD Y1 - 2005/03/02/ PY - 2005 DA - 2005 Mar 02 SP - 396 EP - 399 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 97 IS - 5 SN - 0027-8874, 0027-8874 KW - Risk Abstracts KW - vitamins KW - Liquid chromatography KW - prevention KW - prostate cancer KW - Cancer KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20713221?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Serum+alpha-Tocopherol+and+gamma-Tocopherol+in+Relation+to+Prostate+Cancer+Risk+in+a+Prospective+Study&rft.au=Weinstein%2C+Stephanie+J%3BWright%2C+Margaret+E%3BPietinen%2C+Pirjo%3BKing%2C+Irena%3BTan%2C+Carly%3BTaylor%2C+Philip+R%3BVirtamo%2C+Jarmo%3BAlbanes%2C+Demetrius&rft.aulast=Weinstein&rft.aufirst=Stephanie&rft.date=2005-03-02&rft.volume=97&rft.issue=5&rft.spage=396&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-10-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - vitamins; Liquid chromatography; prevention; prostate cancer; Cancer ER - TY - JOUR T1 - Optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by Candida guilliermondii AN - 856760879; 13858279 AB - The dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/L of xylose, which were fermented with a high inoculum concentration (10 g/L of the yeast Candida guilliermondii IM/UFRJ 50088). The addition of urea reduced the time of conversion (t sub(C)) to 75 h (without nitrogen source addition t sub(C)>127 h), and, consequently, improving the rates of xylitol bioproduction. Fermentator experiments, using the optimized conditions, resulted in enhanced conversion rates, reducing t sub(C) to 30 h. The stability of the yeast in the hydrolysate was also verified in a 480-h cultivation. JF - Applied Biochemistry and Biotechnology AU - Fogel, Rafael AU - Garcia, Rafaela Rodrigues AU - Oliveira, Rebeca da Silva AU - Palacio, Denise Neves Menchero AU - Madeira, Luciana da Silva AU - Pereira, Nei AD - Departmento de Engenharia Bioquimica, Escola de Quimica/CT, Universidade Federal do Rio de Janeiro, CEP: 21949-900, RJ, Brazil, nei@eq.ufrj.br Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 741 EP - 752 PB - Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA VL - 122 IS - 1-3 SN - 0273-2289, 0273-2289 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts C: Algology, Mycology & Protozoology; Biotechnology and Bioengineering Abstracts KW - Bagasse KW - Statistics KW - Xylose KW - Nitrogen sources KW - Xylitol KW - Inoculum KW - Urea KW - Candida guilliermondii KW - Hydrolysis KW - Hydrolysates KW - A 01310:Products of Microorganisms KW - W 30945:Fermentation & Cell Culture KW - K 03320:Cell Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/856760879?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+Biochemistry+and+Biotechnology&rft.atitle=Optimization+of+acid+hydrolysis+of+sugarcane+bagasse+and+investigations+on+its+fermentability+for+the+production+of+xylitol+by+Candida+guilliermondii&rft.au=Fogel%2C+Rafael%3BGarcia%2C+Rafaela+Rodrigues%3BOliveira%2C+Rebeca+da+Silva%3BPalacio%2C+Denise+Neves+Menchero%3BMadeira%2C+Luciana+da+Silva%3BPereira%2C+Nei&rft.aulast=Fogel&rft.aufirst=Rafael&rft.date=2005-03-01&rft.volume=122&rft.issue=1-3&rft.spage=741&rft.isbn=&rft.btitle=&rft.title=Applied+Biochemistry+and+Biotechnology&rft.issn=02732289&rft_id=info:doi/10.1385%2FABAB%3A122%3A1-3%3A0741 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2011-03-01 N1 - Last updated - 2013-12-04 N1 - SubjectsTermNotLitGenreText - Bagasse; Xylose; Statistics; Nitrogen sources; Xylitol; Inoculum; Urea; Hydrolysis; Hydrolysates; Candida guilliermondii DO - http://dx.doi.org/10.1385/ABAB:122:1-3:0741 ER - TY - JOUR T1 - Motor training as treatment in focal hand dystonia. AN - 85380899; pmid-15486996 AB - Focal hand dystonia may arise as a result of aberrant plasticity from excessive repetitive use. Improvement might be possible with appropriate motor training. Focusing on trying to decrease abnormal overflow of movement to fingers not involved in a task, we developed a motor training program for individualized finger movements. Ten patients with writer's cramp participated in the motor training program. Evaluation was done with the Fahn dystonia scale, kinematic analysis of handwriting, transcranial magnetic stimulation (TMS), and electroencephalography (EEG). Clinical improvement of dystonia was significant using the Fahn dystonia scale, and 6 patients reported an improvement in writing. The handwriting analysis showed a trend for improvement after training in simple exercises. There were no changes in cortical excitability measured by TMS and EEG. Whereas this method of motor training for 4 weeks led to mild subjective improvement and some improvement in handwriting, it is not sufficient to reverse motor cortex abnormalities measured by TMS and EEG.2004 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Zeuner, Kirsten E AU - Shill, Holly A AU - Sohn, Young H AU - Molloy, Fiona M AU - Thornton, Bonnie C AU - Dambrosia, James M AU - Hallett, Mark AD - Human Motor Control Section, Medical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-1428, USA. Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 335 EP - 341 VL - 20 IS - 3 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Cerebral Cortex: physiology KW - Dystonia: diagnosis KW - *Dystonia: physiopathology KW - *Dystonia: therapy KW - Dystonic Disorders: physiopathology KW - Dystonic Disorders: therapy KW - Electroencephalography KW - Electromyography: instrumentation KW - Evoked Potentials, Motor: physiology KW - Fingers: physiology KW - *Hand: physiopathology KW - Humans KW - *Magnetics: instrumentation KW - Middle Aged KW - Severity of Illness Index KW - *Teaching: methods KW - Wrist: physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85380899?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Motor+training+as+treatment+in+focal+hand+dystonia.&rft.au=Zeuner%2C+Kirsten+E%3BShill%2C+Holly+A%3BSohn%2C+Young+H%3BMolloy%2C+Fiona+M%3BThornton%2C+Bonnie+C%3BDambrosia%2C+James+M%3BHallett%2C+Mark&rft.aulast=Zeuner&rft.aufirst=Kirsten&rft.date=2005-03-01&rft.volume=20&rft.issue=3&rft.spage=335&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - High-frequency gamma-band activity in the basal temporal cortex during picture-naming and lexical-decision tasks. AN - 85298075; pmid-15800183 AB - Gamma-band activity (GBA) in electroencephalograms (EEGs) has been shown to reflect various cognitive processes. GBA has typically been recorded in the 30-60 Hz range in scalp EEGs. Recently, task-related "high GBA" (HGBA) with frequencies up to 100 Hz has been observed in studies with invasive electrocorticograms (ECoGs). In the present study, we recorded ECoGs from the bilateral basal temporal cortices in a patient with epilepsy and evaluated the task-related HGBA (most prominently in the 80-120 Hz range) accompanying picture-naming and lexical-decision tasks. We examined picture naming using two categories (line drawings of animals and tools). The lexical-decision task was performed using words and pseudowords of two distinct Japanese writing forms, kanji (morphograms) and kana (syllabograms). Task-related HGBA was observed bilaterally during the naming task. Recordings from some electrodes revealed significant differences in HGBA between animal and tool pictures. In contrast to the naming task, there was apparent left dominance in the lexical-decision task. Furthermore, significant differences in HGBA were observed between the Japanese kanji and kana words and between the kanji words and kanji pseudowords. A number of differences in the HGBA observed in the recordings from the basal temporal area were consistent with previous findings from neuroimaging and patient studies and suggest that HGBA is a good correlate of visual cognitive functions. JF - The Journal of Neuroscience AU - Tanji Kazuyo AU - Suzuki, Kyoko AU - Delorme Arnaud AU - Shamoto Hiroshi AU - Nakasato Nobukazu AD - Graduate School of International Cultural Studies, Tohoku University, Sendai 980-8576, Japan. tanjik@mail.nih.gov PY - 2005 SP - 3287 EP - 3293 VL - 25 IS - 13 SN - 0270-6474, 0270-6474 KW - Humans KW - Electroencephalography KW - Decision Making KW - Research Support, Non-U.S. Gov't KW - Photic Stimulation KW - Brain Mapping KW - Comparative Study KW - Evoked Potentials KW - Pattern Recognition, Visual KW - Adult KW - Acoustic Stimulation KW - Laterality KW - Time Factors KW - Male KW - Epilepsy, Temporal Lobe KW - Reaction Time KW - Reading KW - Language KW - High-Frequency Ventilation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85298075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Neuroscience&rft.atitle=High-frequency+gamma-band+activity+in+the+basal+temporal+cortex+during+picture-naming+and+lexical-decision+tasks.&rft.au=Tanji+Kazuyo%3BSuzuki%2C+Kyoko%3BDelorme+Arnaud%3BShamoto+Hiroshi%3BNakasato+Nobukazu&rft.aulast=Tanji+Kazuyo&rft.aufirst=&rft.date=2005-03-01&rft.volume=25&rft.issue=13&rft.spage=3287&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays AN - 839703816; 13860126 AB - We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions, including signal-to-background (S:B) ratios, signal dynamic range, usable spots, and reproducibility. Statistical analysis showed that better results were obtained when spotted, presynthesized long oligonucleotide arrays were blocked with succinic anhydride and hybridized at 42C in the presence of 50% formamide. JF - Molecular Biotechnology AU - Tsai, Mong-Hsun AU - Yan, Hailing AU - Chen, Xi AU - Chandramouli, GVR AU - Zhao, Shuping AU - Coffin, Deborah AU - Coleman, CNorman AU - Mitchell, James B AU - Chuang, Eric Y AD - Radiation Oncology Sciences Program, Radiation Oncology Branch, Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 8717 Grovemont Cr., Room 109J, 20878, Gaithersburg, MD, chuange@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 221 EP - 224 PB - Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA VL - 29 IS - 3 SN - 1073-6085, 1073-6085 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Data processing KW - Statistical analysis KW - DNA microarrays KW - W 30910:Imaging KW - N 14810:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/839703816?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Biotechnology&rft.atitle=Evaluation+of+hybridization+conditions+for+spotted+oligonucleotide-based+DNA+microarrays&rft.au=Tsai%2C+Mong-Hsun%3BYan%2C+Hailing%3BChen%2C+Xi%3BChandramouli%2C+GVR%3BZhao%2C+Shuping%3BCoffin%2C+Deborah%3BColeman%2C+CNorman%3BMitchell%2C+James+B%3BChuang%2C+Eric+Y&rft.aulast=Tsai&rft.aufirst=Mong-Hsun&rft.date=2005-03-01&rft.volume=29&rft.issue=3&rft.spage=221&rft.isbn=&rft.btitle=&rft.title=Molecular+Biotechnology&rft.issn=10736085&rft_id=info:doi/10.1385%2FMB%3A29%3A3%3A221 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2011-01-01 N1 - Last updated - 2012-03-29 N1 - SubjectsTermNotLitGenreText - Data processing; Statistical analysis; DNA microarrays DO - http://dx.doi.org/10.1385/MB:29:3:221 ER - TY - JOUR T1 - Management of neurodegenerative disorders: Parkinson's disease and Alzheimer's disease. AN - 68600798; 16173294 AB - Neurodegenerative disorders result from premature progressive degeneration of specific neurons, and manifest as diseases or syndromes with varied combinations of cognitive, motor, sensory and autonomic dysfunctions. The management involves pharmacotherapy as well as non-pharmacological measures and also to lessen the burden of the care-givers. The medications available for medical treatment are: Levodopa, dopamine agonists, amantadine, anticholinergics, enzyme inhibitors, etc. Advanced Parkinson's disease is concerned with management of motor complications and non-motor complications. Recently surgical treatment is a great option for managing motor complication. Orthostatic hypotension, gait distiurbances, emotional and psychiatric problems, sleep disturbances can be managed and had been discussed in brief. Currently there is no medication available for the cure of Alzheimer's disease. The specific medications claimed to improve patient's well being and cognition include cholinesterase inhibitors, N-methyl-D-aspartate receptor antagonist, anti-oxidants, and anti-amyloid therapy. While medical and surgical treatments for Parkinson's disease have revolutionised the management, still drug therapy for Alzheimer's disease is dismal. JF - Journal of the Indian Medical Association AU - Pal, P K AU - Netravathi, M AD - Department of Neurology, National Institute of Mental Health and Neurosciences, Bangalore 560029. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 168 EP - 70, 172, 174-6 VL - 103 IS - 3 SN - 0019-5847, 0019-5847 KW - Antiparkinson Agents KW - 0 KW - Nootropic Agents KW - Index Medicus KW - Antiparkinson Agents -- adverse effects KW - Physical Therapy Modalities KW - Humans KW - Antiparkinson Agents -- therapeutic use KW - Nootropic Agents -- therapeutic use KW - Antiparkinson Agents -- pharmacology KW - Nootropic Agents -- pharmacology KW - Nootropic Agents -- adverse effects KW - Alzheimer Disease -- complications KW - Alzheimer Disease -- drug therapy KW - Alzheimer Disease -- therapy KW - Parkinson Disease -- complications KW - Parkinson Disease -- drug therapy KW - Parkinson Disease -- surgery UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68600798?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Indian+Medical+Association&rft.atitle=Management+of+neurodegenerative+disorders%3A+Parkinson%27s+disease+and+Alzheimer%27s+disease.&rft.au=Pal%2C+P+K%3BNetravathi%2C+M&rft.aulast=Pal&rft.aufirst=P&rft.date=2005-03-01&rft.volume=103&rft.issue=3&rft.spage=168&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Indian+Medical+Association&rft.issn=00195847&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-17 N1 - Date created - 2005-09-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Occupation and lung cancer risk in Leningrad Province, Russia. AN - 68009877; 16001514 AB - To investigate the association between occupation and lung cancer risk in Leningrad Province, Russia, we identified 540 pathologically diagnosed lung cancer cases (474 males and 66 females) and 582 controls (453 males and 129 females) from the 1993-1998 autopsy records of the 88 state hospitals of the Province. Lifetime occupational histories were obtained from personal records coded according to the standard Russian occupational classification system. Lung cancer risk was increased in workers in the manufacturing industry, particularly in the food industry and wholesale/retail trade and in the miscellaneous manufacturing industry. An increased risk was also found in subjects employed in chemical and metal production for 10 years or more. When we considered the association between specific occupations and lung cancer, waste incineration operators and loaders exhibited an excess risk that increased with employment duration. The present study, which is the first to evaluate the risk of lung cancer by occupation in Russia, suggests the presence in Leningrad Province of exposure in the workplace to lung carcinogens that require further characterization to develop targeted and effective preventive measures. JF - La Medicina del lavoro AU - Baccarelli, A AU - Tretiakova, Maria AU - Gorbanev, S AU - Lomtev, A AU - Klimkina, Irina AU - Tchibissov, V AU - Averkina, Olga AU - Dosemeci, M AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20892, USA. PY - 2005 SP - 142 EP - 154 VL - 96 IS - 2 SN - 0025-7818, 0025-7818 KW - Index Medicus KW - Humans KW - Aged KW - Forms and Records Control KW - Smoking -- epidemiology KW - Cause of Death KW - Russia -- epidemiology KW - Risk KW - Aged, 80 and over KW - Agricultural Workers' Diseases -- epidemiology KW - Adult KW - Case-Control Studies KW - Food Industry KW - Administrative Personnel KW - Middle Aged KW - Male KW - Chemical Industry KW - Female KW - Industry KW - Lung Neoplasms -- epidemiology KW - Lung Neoplasms -- mortality KW - Occupations -- classification UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68009877?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.atitle=Focal+adhesion+kinase+as+a+potential+target+in+arsenic+toxicity.&rft.au=Liu%2C+Jie%3BWaalkes%2C+Michael&rft.aulast=Liu&rft.aufirst=Jie&rft.date=2005-04-01&rft.volume=84&rft.issue=2&rft.spage=212&rft.isbn=&rft.btitle=&rft.title=Toxicological+sciences+%3A+an+official+journal+of+the+Society+of+Toxicology&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-20 N1 - Date created - 2005-07-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A potential role for vascular endothelial growth factor-D as an autocrine growth factor for human breast carcinoma cells. AN - 67790614; 15868899 AB - Vascular endothelial growth factor-D (VEGF-D) binds and activates vascular endothelial growth factor receptor-2 (VEGFR-2), which signals for angiogenesis, and VEGFR-3, which signals for lymphangiogenesis. Besides endothelial cells, VEGFR-2 has been detected on malignant cells, including human breast carcinoma cells. It was examined if ectopic expression of human VEGF-D affected growth of breast carcinoma cell lines in vitro and in vivo. VEGF-D overexpressing clonal MCF-7 and MDA-MB-231 cell lines displayed increased proliferative activities and upregulation of cyclins A, D1 and D3, compared to the vector control. Following subcutaneous inoculation of the MDA-MB-231 cells into nude mice, growth of the VEGF-D overexpressing cells was greatly accelerated. The tumor weight gain was accompanied by increased proliferative activity, cyclin A expression and microvascular density. These findings suggest that VEGF-D functions both as an autocrine growth factor and a stimulator of angiogenesis in breast cancer. JF - Anticancer research AU - Akahane, Manabu AU - Akahane, Takemi AU - Shah, Amy AU - Okajima, Eijiro AU - Thorgeirsson, Unnur P AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, MD 20892-4255, USA. PY - 2005 SP - 701 EP - 707 VL - 25 IS - 2A SN - 0250-7005, 0250-7005 KW - CCND3 protein, human KW - 0 KW - Ccnd3 protein, mouse KW - Cyclin A KW - Cyclin D3 KW - Cyclins KW - DNA, Complementary KW - Vascular Endothelial Growth Factor D KW - Cyclin D1 KW - 136601-57-5 KW - Index Medicus KW - Animals KW - Cyclin A -- genetics KW - Cyclins -- biosynthesis KW - DNA, Complementary -- genetics KW - Cell Growth Processes -- physiology KW - Humans KW - Neovascularization, Pathologic -- metabolism KW - Mice, Nude KW - Mice KW - Cell Line, Tumor KW - Cyclin D1 -- biosynthesis KW - Cyclin D1 -- genetics KW - Neovascularization, Pathologic -- pathology KW - Cyclin A -- biosynthesis KW - Blotting, Western KW - Transfection KW - Enzyme-Linked Immunosorbent Assay KW - Up-Regulation KW - Female KW - Cyclins -- genetics KW - Vascular Endothelial Growth Factor D -- physiology KW - Breast Neoplasms -- genetics KW - Carcinoma -- pathology KW - Carcinoma -- blood supply KW - Breast Neoplasms -- pathology KW - Vascular Endothelial Growth Factor D -- genetics KW - Vascular Endothelial Growth Factor D -- biosynthesis KW - Breast Neoplasms -- metabolism KW - Carcinoma -- metabolism KW - Breast Neoplasms -- blood supply KW - Carcinoma -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67790614?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+research&rft.atitle=A+potential+role+for+vascular+endothelial+growth+factor-D+as+an+autocrine+growth+factor+for+human+breast+carcinoma+cells.&rft.au=Akahane%2C+Manabu%3BAkahane%2C+Takemi%3BShah%2C+Amy%3BOkajima%2C+Eijiro%3BThorgeirsson%2C+Unnur+P&rft.aulast=Akahane&rft.aufirst=Manabu&rft.date=2005-03-01&rft.volume=25&rft.issue=2A&rft.spage=701&rft.isbn=&rft.btitle=&rft.title=Anticancer+research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-09 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Primary chemotherapy with epirubicin and vinorelbine in women with locally advanced breast cancer. AN - 67781614; 15865089 AB - To assess the activity and toxicity of primary chemotherapy with epirubicin (60 mg/m2 every other week) and vinorelbine (25 mg/m2, weekly) plus granulocyte colony-stimulating factor (G-CSF) for 12 weeks, in patients with locally advanced breast cancer in a multicenter setting. Patients with stage IIIA or IIIB breast cancer, not older than 70, were eligible. A two-stage phase II design was applied. Response was assessed clinically, instrumentally and pathologically. Out of 48 enrolled patients, 87.5% received all planned cycles, with a median dose-intensity of 30 mg/m2/week for epirubicin and 23.8 mg/m2/week for vinorelbine. A clinical or instrumental objective response was reached in 42 patients (87.5%, exact 95% CI: 74.7-95.3); significant downstaging was reached in all but one patient; 6 cases had a pathological complete response in the breast, and 2 cases in the lymph nodes too (pathological complete response rate 4.2%, exact 95% CI: 0.5-14.2); a further 2 patients had only microscopic cancer foci at pathological examination of the breast. Radiological tests underestimated the treatment effect on the breast. Toxicity was mild, neutropenia being the most frequent (grade 3-4 in 47% of patients), but never complicated with fever or sepsis. Mild constipation (< or =grade 2) occurred in 35% of patients. Moderate to severe asthenia occurred in 12% of 6 patients. No cardiac toxicity was reported. At 3 years, disease-free survival was 68% and overall survival 81%. Primary chemotherapy with epirubicin every other week, weekly vinorelbine and G-CSF support is highly active and well tolerated in patients with locally advanced breast cancer. JF - Anticancer research AU - Nisticò, Cecilia AU - De Matteis, Andrea AU - Rossi, Emanuela AU - Carnino, Flavio AU - Valenza, Roberto AU - Agostara, Biagio AU - Bria, Emilio AU - Farris, Antonio AU - Cremonesi, Marco AU - D'Ottavio, Anna Maria AU - Vaccaro, Angela AU - Garufi, Carlo AU - Giunta, Salvatore AU - Botti, Claudio AU - Perrone, Francesco AU - Terzoli, Edmondo AD - Department of Medical Oncology, Regina Elena National Cancer Institute, Rome, Italy. cnistico@ifo.it PY - 2005 SP - 1343 EP - 1348 VL - 25 IS - 2B SN - 0250-7005, 0250-7005 KW - Granulocyte Colony-Stimulating Factor KW - 143011-72-7 KW - Epirubicin KW - 3Z8479ZZ5X KW - Vinblastine KW - 5V9KLZ54CY KW - vinorelbine KW - Q6C979R91Y KW - Index Medicus KW - Disease-Free Survival KW - Survival Rate KW - Humans KW - Adult KW - Neutropenia -- chemically induced KW - Aged KW - Middle Aged KW - Mastectomy KW - Granulocyte Colony-Stimulating Factor -- administration & dosage KW - Female KW - Breast Neoplasms -- drug therapy KW - Vinblastine -- analogs & derivatives KW - Vinblastine -- administration & dosage KW - Epirubicin -- administration & dosage KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects KW - Breast Neoplasms -- surgery KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67781614?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+research&rft.atitle=Primary+chemotherapy+with+epirubicin+and+vinorelbine+in+women+with+locally+advanced+breast+cancer.&rft.au=Nistic%C3%B2%2C+Cecilia%3BDe+Matteis%2C+Andrea%3BRossi%2C+Emanuela%3BCarnino%2C+Flavio%3BValenza%2C+Roberto%3BAgostara%2C+Biagio%3BBria%2C+Emilio%3BFarris%2C+Antonio%3BCremonesi%2C+Marco%3BD%27Ottavio%2C+Anna+Maria%3BVaccaro%2C+Angela%3BGarufi%2C+Carlo%3BGiunta%2C+Salvatore%3BBotti%2C+Claudio%3BPerrone%2C+Francesco%3BTerzoli%2C+Edmondo&rft.aulast=Nistic%C3%B2&rft.aufirst=Cecilia&rft.date=2005-03-01&rft.volume=25&rft.issue=2B&rft.spage=1343&rft.isbn=&rft.btitle=&rft.title=Anticancer+research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-10 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Hepatic DNA adduct dosimetry in rats fed tamoxifen: a comparison of methods. AN - 67770734; 15755801 AB - Liver homogenates from rats fed tamoxifen (TAM) in the diet were shared among four different laboratories. TAM-DNA adducts were assayed by high pressure liquid chromatography-electrospray tandem mass spectrometry (HPLC-ES-MS/MS), TAM-DNA chemiluminescence immunoassay (TAM-DNA CIA), and (32)P-postlabeling with either thin layer ((32)P-P-TLC) or liquid chromatography ((32)P-P-HPLC) separation. In the first study, rats were fed a diet containing 500 p.p.m. TAM for 2 months, and the values for measurements of the (E)-alpha-(deoxyguanosin-N(2)-yl)-tamoxifen (dG-N(2)-TAM) adduct in replicate rat livers varied by 3.5-fold when quantified using 'in house' TAM-DNA standards, or other approaches where appropriate. In the second study, rats were fed 0, 50, 250 or 500 p.p.m. TAM for 2 months, and TAM-DNA values were quantified using both 'in house' approaches as well as a newly synthesized [N-methyl-(3)H]TAM-DNA standard that was shared among all the participating groups. In the second study, the total TAM-DNA adduct values varied by 2-fold, while values for the dG-N(2)-TAM varied by 2.5-fold. Ratios of dG-N(2)-TAM:(E)-alpha-(deoxyguanosin-N(2)-yl)-N-desmethyltamoxifen (dG-N(2)-N-desmethyl-TAM) in the second study were approximately 1:1 over the range of doses examined. The study demonstrated a remarkably good agreement for TAM-DNA adduct measurements among the diverse methods employed. JF - Mutagenesis AU - Schild, Laura J AU - Phillips, David H AU - Osborne, Martin R AU - Hewer, Alan AU - Beland, Frederick A AU - Churchwell, Mona I AU - Brown, Karen AU - Gaskell, Margaret AU - Wright, Elizabeth AU - Poirier, Miriam C AD - Carcinogen-DNA Interactions Section, National Cancer Institute, Building 37, Room 4032 NIH, 37 Convent Drive MSC-4255, Bethesda, MD 20892-4255, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 115 EP - 124 VL - 20 IS - 2 SN - 0267-8357, 0267-8357 KW - Antineoplastic Agents, Hormonal KW - 0 KW - DNA Adducts KW - Tamoxifen KW - 094ZI81Y45 KW - Index Medicus KW - Rats KW - Spectrometry, Mass, Electrospray Ionization KW - Animals KW - Rats, Inbred F344 KW - Luminescent Measurements KW - Dose-Response Relationship, Drug KW - Chromatography, Thin Layer KW - Female KW - Chromatography, High Pressure Liquid KW - Tamoxifen -- toxicity KW - DNA Adducts -- analysis KW - Liver -- drug effects KW - DNA Adducts -- chemistry KW - Tamoxifen -- chemistry KW - Antineoplastic Agents, Hormonal -- administration & dosage KW - DNA Adducts -- drug effects KW - Liver -- chemistry KW - Antineoplastic Agents, Hormonal -- chemistry KW - Tamoxifen -- analogs & derivatives KW - Tamoxifen -- administration & dosage KW - Antineoplastic Agents, Hormonal -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67770734?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutagenesis&rft.atitle=Hepatic+DNA+adduct+dosimetry+in+rats+fed+tamoxifen%3A+a+comparison+of+methods.&rft.au=Schild%2C+Laura+J%3BPhillips%2C+David+H%3BOsborne%2C+Martin+R%3BHewer%2C+Alan%3BBeland%2C+Frederick+A%3BChurchwell%2C+Mona+I%3BBrown%2C+Karen%3BGaskell%2C+Margaret%3BWright%2C+Elizabeth%3BPoirier%2C+Miriam+C&rft.aulast=Schild&rft.aufirst=Laura&rft.date=2005-03-01&rft.volume=20&rft.issue=2&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Mutagenesis&rft.issn=02678357&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-08 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Zidovudine induces S-phase arrest and cell cycle gene expression changes in human cells. AN - 67765616; 15784690 AB - Antiretroviral therapy for the human immunodeficiency virus-1 (HIV-1) typically includes two nucleoside reverse transcriptase inhibitors (NRTIs). 3'-Azido-3'-deoxythymidine (AZT, Zidovudine) plus 2'-deoxy-3'-thiacytidine (3TC, Lamivudine) is a combination that is used frequently. The NRTIs are mutagenic nucleoside analogs that become incorporated into DNA and terminate replication. We therefore hypothesized that exposure to this class of drug may alter cell cycle parameters. We used flow cytometry to examine the cell cycle in human epithelioid carcinoma (HeLa) cells exposed to AZT and 3TC alone, as well as a series of AZT/3TC dose combinations: (A) 125.0 microM AZT/12.5 microM 3TC; (B) 250.0 microM AZT/25.0 microM 3TC; and (C) 500 microM AZT/50 microM 3TC. At 24 h, at all doses, there was a good cell viability (>/=68%), and incorporation of AZT into nuclear DNA. Using flow cytometry, a dose-related increase in the percentage of cells in S phase, from 9.5% with no drug, to 36.0% with dose C, was observed in cells exposed for 24 h (P = 0.001, ANOVA). A concomitant decrease in the percentage of cells in G(1) phase, from 82.6% with no drug to 58.5% with dose C, was observed in cells exposed for 24 h (P = 0.017, ANOVA). A similar S phase arrest was seen in cells exposed to 125, 250 and 500 microM AZT alone, but there was no S phase alteration with 50 microM 3TC alone, suggesting that AZT is responsible for the accumulation of cells in S phase. To elucidate the accumulation of cells in S phase and explore the cell cycle gene expression changes induced by AZT and 3TC, we used c-DNA microarray, Cell Cycle Super Array and real-time PCR. There was a strong upregulation of the DNA damage-inducible transcript 3 (DDIT3 or GADD153) in NRTI-exposed cells. In addition, AZT induced an upregulation of cyclin D1 accompanied by a downregulation of the cyclin D1-associated inhibitors P18 and P57, and the G(1)-S check point gene P21, the net effect of which would be to foster a cell progression into S phase. Cyclin A2 was down-regulated in cells exposed to AZT, suggesting a block in S-G(2)-M progression that would also be consistent with the accumulation of cells in S phase. Overall, the study demonstrates that AZT, but not 3TC, causes an arrest of cells in S phase with a consistent alteration in the expression of several cell cycle genes. JF - Mutagenesis AU - Olivero, Ofelia A AU - Tejera, Agueda M AU - Fernandez, Juan J AU - Taylor, Barbara J AU - Das, Shreyasi AU - Divi, Rao L AU - Poirier, Miriam C AD - Carcinogen-DNA Interactions Section, Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, NIH, Bethesda, MD 20892-4255, USA. oliveroo@exchange.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 139 EP - 146 VL - 20 IS - 2 SN - 0267-8357, 0267-8357 KW - Anti-HIV Agents KW - 0 KW - DNA Adducts KW - Mutagens KW - Reverse Transcriptase Inhibitors KW - Lamivudine KW - 2T8Q726O95 KW - Zidovudine KW - 4B9XT59T7S KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Gene Expression -- drug effects KW - Reverse Transcriptase Inhibitors -- administration & dosage KW - Oligonucleotide Array Sequence Analysis KW - HeLa Cells KW - Humans KW - Mutagens -- toxicity KW - Reverse Transcriptase Inhibitors -- toxicity KW - Mutagens -- administration & dosage KW - DNA Adducts -- metabolism KW - Lamivudine -- toxicity KW - Polymerase Chain Reaction KW - Base Sequence KW - DNA -- genetics KW - Lamivudine -- administration & dosage KW - Anti-HIV Agents -- toxicity KW - S Phase -- drug effects KW - Zidovudine -- toxicity KW - Anti-HIV Agents -- administration & dosage KW - Zidovudine -- administration & dosage KW - Cell Cycle -- genetics KW - Cell Cycle -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67765616?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutagenesis&rft.atitle=Zidovudine+induces+S-phase+arrest+and+cell+cycle+gene+expression+changes+in+human+cells.&rft.au=Olivero%2C+Ofelia+A%3BTejera%2C+Agueda+M%3BFernandez%2C+Juan+J%3BTaylor%2C+Barbara+J%3BDas%2C+Shreyasi%3BDivi%2C+Rao+L%3BPoirier%2C+Miriam+C&rft.aulast=Olivero&rft.aufirst=Ofelia&rft.date=2005-03-01&rft.volume=20&rft.issue=2&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=Mutagenesis&rft.issn=02678357&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-08 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Immune reconstitution following hematopoietic progenitor cell transplantation: challenges for the future. AN - 67710792; 15812532 AB - Successful hematopoietic progenitor cell transplantation requires rapid and complete transfer of the donor hematopoietic and immune systems to the host. Whereas the uncontrolled transfer of a nontolerant donor immune system results in GVHD in many cases, strategies which diminish GVHD also diminish immune reconstitution. Thus, the reliable, rapid and safe transfer of immunity from donor to host remains a major challenge for the field. Advances in the understanding of the biology of immune reconstitution have elucidated that thymic-dependent immune reconstitution can restore global immunity, but is especially vulnerable to toxicities associated with transplant. Alternatively, homeostatic peripheral expansion can be exploited for targeted immunity toward pathogens and tumors, but is difficult to manipulate without exacerbating GVHD risk. New translatable strategies are needed to safely augment one or both of these pathways in the setting of allogeneic hematopoietic progenitor cell transplantation. JF - Bone marrow transplantation AU - Fry, T J AU - Mackall, C L AD - Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - S53 EP - S57 VL - 35 Suppl 1 SN - 0268-3369, 0268-3369 KW - Index Medicus KW - Animals KW - Humans KW - Graft Survival KW - Transplantation, Autologous KW - Graft vs Host Disease -- prevention & control KW - Hematopoietic Stem Cell Transplantation -- trends KW - Recovery of Function KW - Hematopoietic Stem Cell Transplantation -- methods KW - Immune System UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67710792?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.atitle=Green+tea+consumption%2C+genetic+susceptibility%2C+PAH-rich+smoky+coal%2C+and+the+risk+of+lung+cancer&rft.au=Bonner%2C+M+R%3BRothman%2C+N%3BMumford%2C+J+L%3BHe%2C+X%3BShen%2C+M%3BWelch%2C+R%3BYeager%2C+M%3BChanock%2C+S%3BCaporaso%2C+N%3BLan%2C+Q&rft.aulast=Bonner&rft.aufirst=M&rft.date=2005-04-04&rft.volume=582&rft.issue=1-2&rft.spage=53&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.issn=13835718&rft_id=info:doi/10.1016%2Fj.mrgentox.2004.12.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-30 N1 - Date created - 2005-04-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Influence of functional group substitutions on the carcinogenicity of anthraquinone in rats and mice: analysis of long-term bioassays by the National Cancer Institute and the National Toxicology Program. AN - 67700199; 15804751 AB - The carcinogenic activities of anthraquinone and six derivatives were compared and contrasted. Studies included representatives of amino, alkyl, nitro, hydroxy, or halogen-containing anthraquinones, with the purpose of uncovering general structure-activity relationships. Anthraquinone, 2-aminoanthraquinone, 1-amino-2-methylanthraquinone, 2-methyl-1-nitroanthraquinone,1-amino-2,4-dibromoanthraquinone, 1,4,5,8-tetraaminoanthraquinone, and 1,3,8-trihydroxy-6-methylanthraquinone (of varying purities) were administered via feed to Fischer 344/N rats and B6C3F, mice. In rats, anthraquinone induced tumors in the liver, kidney, and urinary bladder. A 2-amino substitution narrowed the carcinogenicity to the liver, while multiple amino substitutions led to a carcinogenic response in the urinary bladder alone. A methyl substitution ortho to a 1-aminogroup preserved the hepatic and renal neoplasms seen with the parent anthraquinone, but did not induce urinary bladder tumors; amino or bromo substitutions para to a 1-amino group were related to urinary bladder neoplasms. The intestine may have been a target organ for bromine-substituted anthraquinones. The presence of a nitro group altered the targets of carcinogenicity, and skin tumors may have been associated with this particular functional group in both rats and mice. Over-all for mice, the findings were somewhat different and limited by the small number of common target organs. The parent anthraquinone was clearly carcinogenic only to the liver. There were no other effects of single amino substitutions, in the presence or absence of an additional methyl group, on the carcinogenicity or the site of carcinogenesis of anthraquinone in mice. Multiple amino substitutions diminished, while bromine substitutions enhanced the carcinogenicity induced by anthraquinone and extended the target organs to include forestomach and lung. JF - Journal of toxicology and environmental health. Part B, Critical reviews AU - Doi, Adriana M AU - Irwin, Richard D AU - Bucher, John R AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA. doi@niehs.nih.gov PY - 2005 SP - 109 EP - 126 VL - 8 IS - 2 SN - 1093-7404, 1093-7404 KW - Anthraquinones KW - 0 KW - Carcinogens KW - Index Medicus KW - Rats KW - Animals KW - Biological Assay KW - Mice KW - Structure-Activity Relationship KW - Neoplasms, Experimental -- chemically induced KW - Carcinogens -- toxicity KW - Anthraquinones -- metabolism KW - Anthraquinones -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67700199?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+toxicology+and+environmental+health.+Part+B%2C+Critical+reviews&rft.atitle=Influence+of+functional+group+substitutions+on+the+carcinogenicity+of+anthraquinone+in+rats+and+mice%3A+analysis+of+long-term+bioassays+by+the+National+Cancer+Institute+and+the+National+Toxicology+Program.&rft.au=Doi%2C+Adriana+M%3BIrwin%2C+Richard+D%3BBucher%2C+John+R&rft.aulast=Doi&rft.aufirst=Adriana&rft.date=2005-03-01&rft.volume=8&rft.issue=2&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=Journal+of+toxicology+and+environmental+health.+Part+B%2C+Critical+reviews&rft.issn=10937404&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-28 N1 - Date created - 2005-04-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Heterologous protein production from the inducible MET25 promoter in Saccharomyces cerevisiae. AN - 67573523; 15801808 AB - Heterologous protein production late in Saccharomyces cerevisiae fermentations is often desirable because it may help avoid the unintentional selection of more rapidly growing, non-protein-expressing cells or allow for the expression of toxic proteins. Here, we describe the use of the MET25 promoter for the production of human serum albumin (HSA) and HSA-fusion proteins in S. cerevisiae. In media lacking methionine, the MET25 promoter yielded high expression levels of HSA and HSA fused to human glucagon, human growth hormone, human interferon alpha, and human interleukin-2. More importantly, we have shown that this system can be used to delay heterologous protein production until late log phase of the growth of the culture and does not require the addition of an exogenous inducer. JF - Biotechnology progress AU - Solow, Steven P AU - Sengbusch, Jennifer AU - Laird, Michael W AD - Human Genome Sciences, Inc., Rockville, Maryland 20850, USA. solow@ncbi.nlm.nih.gov PY - 2005 SP - 617 EP - 620 VL - 21 IS - 2 SN - 8756-7938, 8756-7938 KW - Recombinant Fusion Proteins KW - 0 KW - Saccharomyces cerevisiae Proteins KW - Methionine KW - AE28F7PNPL KW - Index Medicus KW - Recombinant Fusion Proteins -- metabolism KW - Recombinant Fusion Proteins -- biosynthesis KW - Electrophoresis, Polyacrylamide Gel KW - Recombinant Fusion Proteins -- genetics KW - Methionine -- metabolism KW - Plasmids KW - Saccharomyces cerevisiae Proteins -- metabolism KW - Promoter Regions, Genetic KW - Saccharomyces cerevisiae Proteins -- genetics KW - Saccharomyces cerevisiae Proteins -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67573523?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biotechnology+progress&rft.atitle=Heterologous+protein+production+from+the+inducible+MET25+promoter+in+Saccharomyces+cerevisiae.&rft.au=Solow%2C+Steven+P%3BSengbusch%2C+Jennifer%3BLaird%2C+Michael+W&rft.aulast=Solow&rft.aufirst=Steven&rft.date=2005-03-01&rft.volume=21&rft.issue=2&rft.spage=617&rft.isbn=&rft.btitle=&rft.title=Biotechnology+progress&rft.issn=87567938&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-06 N1 - Date created - 2005-04-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Analysis of target genes induced by IL-13 cytotoxin in human glioblastoma cells. AN - 67569226; 15803373 AB - IL-13 cytotoxin comprised of IL-13 and a mutated form of Pseudomonas exotoxin (fusion protein termed IL-13-PE38QQR) has been shown to inhibit protein synthesis leading to necrotic and apoptotic cell death in glioblastoma cells that express high levels of interleukin-13 receptors (IL-13R). To identify target genes of cell death and other cellular genes with IL-13 receptors in glioblastoma cells, we utilized the cDNA microarrays to analyze global gene expression profiles after IL-13 cytotoxin and IL-13 treatment. IL-13 cytotoxin mediated cytotoxicity to U251 cells in a dose-dependent manner. Hierarchical cluster analysis of differentially expressed genes in U251 glioma cells at different time points after IL-13 cytotoxin treatment showed three major groups, each representing a specific expression pattern. Randomly selected differentially expressed genes from each group were confirmed by RT-PCR analysis. Most down-regulated genes belong to cell adhesion, motility, angiogenesis, DNA repair, and metabolic pathways. While up-regulated genes belong to cell cycle arrest, apoptosis, signaling and various metabolic pathways. Unexpectedly, at early time points, both IL-13 and IL-13 cytotoxin induced several genes belonging to different pathways most notably IL-8, DIO2, END1, and ALDH1A3 indicating that these genes are early response genes and their products may be associated with IL-13R. In addition, IL-13 cytotoxin induced IL-13Ralpha2 mRNA expression during the treatment in glioma cells. Our results indicate that novel cellular genes are involved with IL-13 receptors and that IL-13 cytotoxin induced cell death involves various target genes in human glioblastoma cells. On going studies will determine the role of associated genes and their products in the IL-13R functions in glioma cells. JF - Journal of neuro-oncology AU - Han, Jing AU - Yang, Liming AU - Puri, Raj K AD - Laboratory of Molecular Tumor Biology, CBER/NCI Genomics Program, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, CBER/FDA, NIH, Bethesda, MD 20892, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 35 EP - 46 VL - 72 IS - 1 SN - 0167-594X, 0167-594X KW - Antineoplastic Agents KW - 0 KW - Bacterial Toxins KW - Cytotoxins KW - Exotoxins KW - IL13-PE38QQR KW - IL13RA1 protein, human KW - Immunotoxins KW - Interleukin-13 KW - Interleukin-13 Receptor alpha1 Subunit KW - Neoplasm Proteins KW - Receptors, Interleukin KW - Receptors, Interleukin-13 KW - Recombinant Fusion Proteins KW - Virulence Factors KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - toxA protein, Pseudomonas aeruginosa KW - EC 2.4.2.31 KW - Index Medicus KW - Receptors, Interleukin -- metabolism KW - Gene Silencing KW - Dose-Response Relationship, Drug KW - Humans KW - Apoptosis -- physiology KW - Bacterial Toxins -- pharmacology KW - Cell Line, Tumor KW - Cytotoxins -- pharmacology KW - ADP Ribose Transferases -- pharmacology KW - Cell Death -- drug effects KW - Gene Expression Regulation, Neoplastic -- drug effects KW - Virulence Factors -- pharmacology KW - Gene Expression Profiling KW - Apoptosis -- drug effects KW - Receptors, Interleukin -- drug effects KW - Antineoplastic Agents -- pharmacology KW - Cluster Analysis KW - Neoplasm Proteins -- drug effects KW - Glioblastoma -- genetics KW - Exotoxins -- pharmacology KW - Brain Neoplasms -- drug therapy KW - Brain Neoplasms -- genetics KW - Glioblastoma -- drug therapy KW - Immunotoxins -- pharmacology KW - Neoplasm Proteins -- metabolism KW - Interleukin-13 -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67569226?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Modeling+spontaneous+activity+in+the+developing+spinal+cord+using+activity-dependent+variations+of+intracellular+chloride.&rft.au=Marchetti%2C+Cristina%3BTabak%2C+Joel%3BChub%2C+Nikolai%3BO%27Donovan%2C+Michael+J%3BRinzel%2C+John&rft.aulast=Marchetti&rft.aufirst=Cristina&rft.date=2005-04-06&rft.volume=25&rft.issue=14&rft.spage=3601&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-04-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The fetal basis of adult disease: Role of environmental exposures--introduction. AN - 67566105; 15751038 JF - Birth defects research. Part A, Clinical and molecular teratology AU - Heindel, Jerrold J AD - Division of Extramural Research and Training, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Sciences, Research Triangle Park, North Carolina, USA. heindelj@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 131 EP - 132 VL - 73 IS - 3 SN - 1542-0752, 1542-0752 KW - Index Medicus KW - Animals KW - Humans KW - Gene Expression Regulation, Developmental -- drug effects KW - Female KW - Pregnancy KW - Disease Susceptibility -- etiology KW - Disease Susceptibility -- embryology KW - Environmental Exposure -- adverse effects KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67566105?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.atitle=The+fetal+basis+of+adult+disease%3A+Role+of+environmental+exposures--introduction.&rft.au=Heindel%2C+Jerrold+J&rft.aulast=Heindel&rft.aufirst=Jerrold&rft.date=2005-03-01&rft.volume=73&rft.issue=3&rft.spage=131&rft.isbn=&rft.btitle=&rft.title=Birth+defects+research.+Part+A%2C+Clinical+and+molecular+teratology&rft.issn=15420752&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-23 N1 - Date created - 2005-03-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selective ablation of nociceptive neurons for elimination of hyperalgesia and neurogenic inflammation. AN - 67559095; 15796388 AB - Neuropathic pain is mediated by nociceptive neurons that selectively express the vanilloid receptor 1 (VR1). Resiniferatoxin (RTX) is an excitotoxic VR1 agonist that causes destruction of VR1-positive neurons. To determine whether RTX can be used to ablate VR1-positive neurons selectively and to eliminate hyperalgesia and neurogenic inflammation without affecting tactile sensation and motor function, the authors infused it unilaterally into the trigeminal ganglia in Rhesus monkeys. Either RTX (three animals) or vehicle (one animal) was directly infused (20 microl) into the right trigeminal ganglion in Rhesus monkeys. Animals were tested postoperatively at 1, 4, and 7 weeks thereafter for touch and pain perception in the trigeminal distribution (application of saline and capsaicin to the cornea). The number of eye blinks, eye wipes, and duration of squinting were recorded. Neurogenic inflammation was tested using capsaicin cream. Animals were killed 4 (one monkey) and 12 (three monkeys) weeks postinfusion. Histological and immunohistochemical analyses were performed. Throughout the duration of the study, response to high-intensity pain stimulation (capsaicin) was selectively and significantly reduced (p < 0.001, RTX-treated compared with vehicle-treated eye [mean +/- standard deviation]): blinks, 25.7 +/- 4.4 compared with 106.6 +/- 20.8; eye wipes, 1.4 +/- 0.8 compared with 19.3 +/- 2.5; and squinting, 1.4 +/- 0.6 seconds compared with 11.4 +/- 1.6 seconds. Normal response to sensation was maintained. Animals showed no neurological deficit or sign of toxicity. Neurogenic inflammation was blocked on the RTX-treated side. Immunohistochemical analysis of the RTX-treated ganglia showed selective elimination of VR1-positive neurons. Nociceptive neurons can be selectively ablated by intraganglionic RTX infusion, resulting in the elimination of high-intensity pain perception and neurogenic inflammation while maintaining normal sensation and motor function. Analysis of these findings indicated that intraganglionic RTX infusion may provide a new treatment for pain syndromes such as trigeminal neuralgia as well as others. JF - Journal of neurosurgery AU - Tender, Gabriel C AU - Walbridge, Stuart AU - Olah, Zoltan AU - Karai, Laszlo AU - Iadarola, Michael AU - Oldfield, Edward H AU - Lonser, Russell R AD - Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-1414, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 522 EP - 525 VL - 102 IS - 3 SN - 0022-3085, 0022-3085 KW - Diterpenes KW - 0 KW - resiniferatoxin KW - A5O6P1UL4I KW - Capsaicin KW - S07O44R1ZM KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Trigeminal Ganglion -- drug effects KW - Macaca mulatta KW - Immunohistochemistry KW - Capsaicin -- pharmacology KW - Neurogenic Inflammation -- pathology KW - Hyperalgesia -- pathology KW - Diterpenes -- administration & dosage KW - Neurogenic Inflammation -- drug therapy KW - Neurons, Afferent -- drug effects KW - Diterpenes -- therapeutic use KW - Nociceptors -- drug effects KW - Hyperalgesia -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67559095?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurosurgery&rft.atitle=Selective+ablation+of+nociceptive+neurons+for+elimination+of+hyperalgesia+and+neurogenic+inflammation.&rft.au=Tender%2C+Gabriel+C%3BWalbridge%2C+Stuart%3BOlah%2C+Zoltan%3BKarai%2C+Laszlo%3BIadarola%2C+Michael%3BOldfield%2C+Edward+H%3BLonser%2C+Russell+R&rft.aulast=Tender&rft.aufirst=Gabriel&rft.date=2005-03-01&rft.volume=102&rft.issue=3&rft.spage=522&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurosurgery&rft.issn=00223085&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-15 N1 - Date created - 2005-03-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Health status and plasma dioxin levels in chloracne cases 20 years after the Seveso, Italy accident. AN - 67546607; 15787814 AB - The Seveso, Italy accident of 1976 exposed a large population to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or simply dioxin). The accident resulted, mostly among children, in one of the largest ever-reported outbreaks of chloracne, the typical skin disorder due to halogenated-hydrocarbon compounds. Approximately 20 years after the accident, we conducted an epidemiological study in Seveso to investigate (a) the health status of chloracne cases; (b) TCDD-chloracne exposure-response relationship; and (c) factors modifying TCDD toxicity. From 1993 to 1998, we recruited 101 chloracne cases and 211 controls. Trained interviewers administered a structured questionnaire assessing, among other epidemiological variables, information on an extensive list of diseases. During the interview, individual pigmentary characteristics were determined. We measured plasma TCDD levels using high-resolution gas chromatography/mass spectrometry. Plasma TCDD was still elevated (> 10 ppt) in 78 (26.6%) of the 293 subjects with adequate plasma samples, particularly in females, in subjects who had eaten home-grown animals, and in individuals with older age, higher body mass index and residence near the accident site. After 20 years, health conditions of chloracne cases were similar to those of controls from the Seveso area. Elevated plasma TCDD was associated with chloracne [odds ratio (OR) = 3.7, 95% confidence interval (CI) 1.6-8.8, adjusted for age, sex and residence]. Chloracne risk was higher in subjects younger than 8 years at the accident (OR = 7.4, 95% CI 1.8-30.3) and, contrary to previous hypotheses, did not increase at puberty onset or in teenage years. Subjects with elevated TCDD levels and light hair colour had higher relative odds of chloracne (OR = 9.2, 95% CI 2.6-32.5). Dioxin toxicity in chloracne cases was confined to the acute dermatotoxic effects. Chloracne occurrence appeared related to younger age and light hair colour. Age-related dioxin elimination or dilution must be taken into account in interpreting these results. JF - The British journal of dermatology AU - Baccarelli, A AU - Pesatori, A C AU - Consonni, D AU - Mocarelli, P AU - Patterson, D G AU - Caporaso, N E AU - Bertazzi, P A AU - Landi, M T AD - Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, DHHS, Behtesda, MD 20892-7236, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 459 EP - 465 VL - 152 IS - 3 SN - 0007-0963, 0007-0963 KW - Polychlorinated Dibenzodioxins KW - 0 KW - Index Medicus KW - Age Factors KW - Hair Color KW - Humans KW - Environmental Exposure -- analysis KW - Child KW - Body Mass Index KW - Child, Preschool KW - Infant KW - Risk Factors KW - Adult KW - Case-Control Studies KW - Middle Aged KW - Italy -- epidemiology KW - Adolescent KW - Male KW - Female KW - Health Status KW - Polychlorinated Dibenzodioxins -- toxicity KW - Polychlorinated Dibenzodioxins -- blood KW - Acne Vulgaris -- chemically induced KW - Acne Vulgaris -- epidemiology KW - Occupational Diseases -- chemically induced KW - Accidents, Occupational UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67546607?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+British+journal+of+dermatology&rft.atitle=Health+status+and+plasma+dioxin+levels+in+chloracne+cases+20+years+after+the+Seveso%2C+Italy+accident.&rft.au=Baccarelli%2C+A%3BPesatori%2C+A+C%3BConsonni%2C+D%3BMocarelli%2C+P%3BPatterson%2C+D+G%3BCaporaso%2C+N+E%3BBertazzi%2C+P+A%3BLandi%2C+M+T&rft.aulast=Baccarelli&rft.aufirst=A&rft.date=2005-03-01&rft.volume=152&rft.issue=3&rft.spage=459&rft.isbn=&rft.btitle=&rft.title=The+British+journal+of+dermatology&rft.issn=00070963&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-03-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CONF T1 - Introduction: new dynamics of HIV risk among drug-using men who have sex with men. AN - 67525389; 15738326 JF - Journal of urban health : bulletin of the New York Academy of Medicine AU - Lambert, Elizabeth AU - Normand, Jacques AU - Stall, Ron AU - Aral, Sevgi AU - Vlahov, David Y1 - 2005/03// PY - 2005 DA - March 2005 SP - i1 EP - i8 VL - 82 IS - 1 Suppl 1 KW - Index Medicus KW - Sexual Behavior KW - Risk-Taking KW - Public Health Practice KW - Humans KW - Urban Population KW - Male KW - HIV Infections -- transmission KW - HIV Infections -- prevention & control KW - Substance-Related Disorders -- complications KW - HIV Infections -- epidemiology KW - Homosexuality, Male -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67525389?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Substrate+specificity+of+the+human+protein+phosphatase+2Cdelta%2C+Wip1.&rft.au=Yamaguchi%2C+Hiroshi%3BMinopoli%2C+Giuseppina%3BDemidov%2C+Oleg+N%3BChatterjee%2C+Deb+K%3BAnderson%2C+Carl+W%3BDurell%2C+Stewart+R%3BAppella%2C+Ettore&rft.aulast=Yamaguchi&rft.aufirst=Hiroshi&rft.date=2005-04-12&rft.volume=44&rft.issue=14&rft.spage=5285&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-15 N1 - Date created - 2005-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Thr105Ile, a functional polymorphism of histamine N-methyltransferase, is associated with alcoholism in two independent populations. AN - 67514388; 15770103 AB - Histamine is expressed in cortical and limbic areas that are involved in emotion and cognition and modulates these behaviors. H1 receptor antagonists are sedative. Histamine N-methyltransferase (HNMT) catalyzes the Ntau methylation of histamine, the sole pathway for termination of the neurotransmitter action of histamine in mammalian brain. A common and functionally significant polymorphism, a C314T transition in exon 4 of the HNMT gene results in a Thr105Ile substitution of the protein encoded. The Thr105 allele is associated with approximately 2-fold higher enzyme activity, leading to the prediction that it might be associated with diminished histamine levels, resulting in differences in anxiety, cognition, and sedation that play important roles in alcoholism. In two ethnically distinct populations, we tested whether the Thr105Ile polymorphism was associated with alcoholism and with harm avoidance, a dimensional measure of anxious personality. A 5' exonuclease assay (TaqMan) was used to genotype Thr105Ile in psychiatrically interviewed Finnish Caucasian (n = 218) and Plains American Indian (n = 186) alcoholics, along with ethnically matched, psychiatrically interviewed, controls (Finns: n = 313, Plains Indian: n = 140). Ile105 allele frequencies were significantly lower in alcoholics compared with nonalcoholics in both populations (Finns: 0.12 vs. 0.17, chi(2) = 6, p = 0.015; Plains Indians: 0.03 vs. 0.08, chi(2) = 5, p = 0.023). Genotype distributions also differed significantly. In Finns, Ile105 showed borderline significance for an association with lower harm avoidance (p = 0.070) after correcting for alcoholism diagnosis. Decreased levels of brain histamine consequent to the Thr105 allele may result in higher levels of anxiety and, as a consequence, vulnerability to alcoholism. JF - Alcoholism, clinical and experimental research AU - Oroszi, Gabor AU - Enoch, Mary-Anne AU - Chun, Jeffrey AU - Virkkunen, Matti AU - Goldman, David AD - Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, USA. goroszi@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 303 EP - 309 VL - 29 IS - 3 SN - 0145-6008, 0145-6008 KW - Histamine N-Methyltransferase KW - EC 2.1.1.8 KW - Phosphodiesterase I KW - EC 3.1.4.1 KW - Index Medicus KW - Anxiety -- psychology KW - Gene Frequency KW - Personality Tests KW - Polymorphism, Genetic -- genetics KW - Humans KW - Phosphodiesterase I -- genetics KW - Genotype KW - Alleles KW - Indians, North American KW - Psychiatric Status Rating Scales KW - Anxiety -- genetics KW - European Continental Ancestry Group KW - Adult KW - United States -- epidemiology KW - Finland -- epidemiology KW - Female KW - Male KW - Histamine N-Methyltransferase -- genetics KW - Alcoholism -- genetics KW - Alcoholism -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67514388?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Thr105Ile%2C+a+functional+polymorphism+of+histamine+N-methyltransferase%2C+is+associated+with+alcoholism+in+two+independent+populations.&rft.au=Oroszi%2C+Gabor%3BEnoch%2C+Mary-Anne%3BChun%2C+Jeffrey%3BVirkkunen%2C+Matti%3BGoldman%2C+David&rft.aulast=Oroszi&rft.aufirst=Gabor&rft.date=2005-03-01&rft.volume=29&rft.issue=3&rft.spage=303&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-27 N1 - Date created - 2005-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The conventional nonsteroidal anti-inflammatory drug sulindac sulfide arrests ovarian cancer cell growth via the expression of NAG-1/MIC-1/GDF-15. AN - 67513850; 15767558 AB - Although the chemopreventive and antitumorigenic activities of nonsteroidal anti-inflammatory drug (NSAID) against colorectal cancer are well established, the molecular mechanisms responsible for these properties in ovarian cancer have not been elucidated. Therefore, there is an urgent need to develop mechanism-based approaches for the management of ovarian cancer. To this end, the effect of several NSAIDs on ovarian cancer cells was investigated as assessed by the induction of NAG-1/MIC-1/GDF-15, a proapoptotic gene belonging to the transforming growth factor-beta superfamily. Sulindac sulfide was the most significant NSAID activated gene 1 (NAG-1) inducer and its expression was inversely associated with cell viability as determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. This growth suppression by sulindac sulfide was recovered by transfection of NAG-1 small interfering RNA. These results indicate that NAG-1 is one of the genes responsible for growth suppression by sulindac sulfide. Furthermore, we observed down-regulation of p21 WAF1/CIP1 by introduction of NAG-1 small interfering RNA into sulindac sulfide-treated cells. In addition, to elucidate other potential molecular mechanisms involved in sulindac sulfide treatment of ovarian cancer cells, we did a membrane-based microarray experiment. We found that cyclin D1, MMP-1, PI3KR1, and uPA were down-regulated by sulindac sulfide. In conclusion, a novel molecular mechanism is proposed to explain the experimental results and provide a rationale for the chemopreventive activity of NSAIDs in ovarian cancer. JF - Molecular cancer therapeutics AU - Kim, Jong-Sik AU - Baek, Seung Joon AU - Sali, Tina AU - Eling, Thomas E AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, MD: E4-09, P.O. Box 12233, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 487 EP - 493 VL - 4 IS - 3 SN - 1535-7163, 1535-7163 KW - Anti-Inflammatory Agents, Non-Steroidal KW - 0 KW - CDKN1A protein, human KW - Cell Cycle Proteins KW - Coloring Agents KW - Cyclin-Dependent Kinase Inhibitor p21 KW - Cytokines KW - GDF15 protein, human KW - Growth Differentiation Factor 15 KW - RNA, Small Interfering KW - Tetrazolium Salts KW - Thiazoles KW - Cyclin D1 KW - 136601-57-5 KW - Sulindac KW - 184SNS8VUH KW - sulindac sulfide KW - 6UVA8S2DEY KW - Luciferases KW - EC 1.13.12.- KW - thiazolyl blue KW - EUY85H477I KW - Index Medicus KW - Coloring Agents -- pharmacology KW - Apoptosis KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Luciferases -- metabolism KW - Cell Line, Tumor KW - Cytokines -- metabolism KW - Reverse Transcriptase Polymerase Chain Reaction KW - Tetrazolium Salts -- pharmacology KW - RNA, Small Interfering -- metabolism KW - Cell Cycle Proteins -- metabolism KW - Cell Survival KW - Thiazoles -- pharmacology KW - Promoter Regions, Genetic KW - Blotting, Western KW - Cyclin D1 -- metabolism KW - Down-Regulation KW - Up-Regulation KW - Time Factors KW - Female KW - Ovarian Neoplasms -- metabolism KW - Sulindac -- pharmacology KW - Sulindac -- analogs & derivatives KW - Ovarian Neoplasms -- drug therapy KW - Anti-Inflammatory Agents, Non-Steroidal -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67513850?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+therapeutics&rft.atitle=The+conventional+nonsteroidal+anti-inflammatory+drug+sulindac+sulfide+arrests+ovarian+cancer+cell+growth+via+the+expression+of+NAG-1%2FMIC-1%2FGDF-15.&rft.au=Kim%2C+Jong-Sik%3BBaek%2C+Seung+Joon%3BSali%2C+Tina%3BEling%2C+Thomas+E&rft.aulast=Kim&rft.aufirst=Jong-Sik&rft.date=2005-03-01&rft.volume=4&rft.issue=3&rft.spage=487&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Developmental phases of inflammation-induced massive lymphoid hyperplasia and extensive changes in epithelium in an experimental model of allergy: implications for a direct link between inflammation and carcinogenesis. AN - 67513653; 15767829 AB - Direct evidence that inflammation is linked to carcinogenesis has yet to be established. Very few data are available on the developmental phases of inflammation-induced immune dysfunction that may lead to tumorigenesis. In a series of studies conducted in the 1980s and 1990s, an experimental model of acute and chronic inflammation was established in guinea pig conjunctiva by topical application of fluoresceinyl ovalbumin (FLOA) for up to 30 months. In this updated report, some of the findings are reanalyzed and expanded to identify that at lease 3 developmental phases were involved during the entire course of inflammatory responses including (1) an acute response (phase A) involving IgE-mast cell sensitization and degranulation; (2) an intermediate phase (phase B), a desensitization phenomenon and loss of mast cell function and neovascularization; (3) a chronic response (phase C) and induction of massive lymphoid hyperplasia, follicular formation with germinal centers, increased swollen goblet cells, extensive epithelial thickening and thinning, and angiogenesis. The results suggest evidence of a direct association between inflammation and the development of tumor-like lesions in lymphoid tissues and extensive changes in adjacent epithelia. Confirmation that inflammation induces irreversible changes in lymphoid and epithelial tissues leading to lymphoid tumorigenesis and/or carcinogenesis requires further studies. Understanding the developmental phases of immune dysfunction may provide unique opportunities for diagnosis and treatment of inflammatory diseases, autoimmune disorders, and cancers including lymphomas associated with Sjogren syndrome, squamous cell carcinoma of the conjunctiva, and other lymphomas or epithelial cancers. It is suggested that inflammatory mediators are ideal targets (biomarkers) for diagnosis, chemoprevention, and therapy for several cancers. JF - American journal of therapeutics AU - Khatami, Mahin AD - University of Pennsylvania School of Medicine, Philadelphia, PA, USA. Khatamim@mail.nih.gov PY - 2005 SP - 117 EP - 126 VL - 12 IS - 2 SN - 1075-2765, 1075-2765 KW - Inflammation Mediators KW - 0 KW - Index Medicus KW - Acute Disease KW - Animals KW - Mast Cells -- immunology KW - Hypersensitivity, Immediate -- immunology KW - Cell Degranulation KW - Hyperplasia -- immunology KW - Guinea Pigs KW - Antibody Formation KW - Disease Models, Animal KW - Haplorhini KW - Inflammation Mediators -- metabolism KW - Neovascularization, Pathologic -- immunology KW - Animals, Newborn KW - Neovascularization, Pathologic -- etiology KW - Hyperplasia -- etiology KW - Dogs KW - Chronic Disease KW - Epithelium -- pathology KW - Lymphoid Tissue -- pathology KW - Conjunctivitis, Allergic -- pathology KW - Lymphoid Tissue -- immunology KW - Conjunctivitis, Allergic -- immunology KW - Conjunctivitis, Allergic -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67513653?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+therapeutics&rft.atitle=Developmental+phases+of+inflammation-induced+massive+lymphoid+hyperplasia+and+extensive+changes+in+epithelium+in+an+experimental+model+of+allergy%3A+implications+for+a+direct+link+between+inflammation+and+carcinogenesis.&rft.au=Khatami%2C+Mahin&rft.aulast=Khatami&rft.aufirst=Mahin&rft.date=2005-03-01&rft.volume=12&rft.issue=2&rft.spage=117&rft.isbn=&rft.btitle=&rft.title=American+journal+of+therapeutics&rft.issn=10752765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Interfacial inhibition of macromolecular interactions: nature's paradigm for drug discovery. AN - 67490217; 15749159 AB - One of nature's strategies for interfering with molecular interactions is to trap macromolecules in transition states with their partners in dead-end complexes that are unable to complete their biological function. This type of inhibition, which we refer to as "interfacial inhibition", is illustrated by two natural inhibitors, brefeldin A (BFA) and camptothecin (CPT), whose modes of action have been elucidated fully in structural studies. Interfacial inhibition occurs at the protein-protein interface in the case of BFA and at the protein-DNA interface in the case of CPT. In both systems, the drugs take advantage of transient structural and energetic conditions created by the macromolecular complex, which give rise to "hot-spots" for drug binding. In addition to these examples, several natural compounds such as forskolin, tubulin inhibitors and immunophilins target protein interfaces. We propose that interfacial inhibition is a paradigm for the discovery of drugs that interfere with macromolecular complexes. JF - Trends in pharmacological sciences AU - Pommier, Yves AU - Cherfils, Jacqueline AD - Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA. Pommier@nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 138 EP - 145 VL - 26 IS - 3 SN - 0165-6147, 0165-6147 KW - Macromolecular Substances KW - 0 KW - Pharmaceutical Preparations KW - Index Medicus KW - Protein Binding -- physiology KW - Animals KW - Protein Binding -- drug effects KW - Humans KW - Drug Interactions -- physiology KW - Pharmaceutical Preparations -- metabolism KW - Pharmaceutical Preparations -- chemistry KW - Macromolecular Substances -- antagonists & inhibitors KW - Macromolecular Substances -- metabolism KW - Macromolecular Substances -- chemistry KW - Drug Design UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67490217?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Trends+in+pharmacological+sciences&rft.atitle=Interfacial+inhibition+of+macromolecular+interactions%3A+nature%27s+paradigm+for+drug+discovery.&rft.au=Pommier%2C+Yves%3BCherfils%2C+Jacqueline&rft.aulast=Pommier&rft.aufirst=Yves&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=138&rft.isbn=&rft.btitle=&rft.title=Trends+in+pharmacological+sciences&rft.issn=01656147&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-26 N1 - Date created - 2005-03-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - No phenotype associated with established lipopolysaccharide model for cerebral palsy. AN - 67487182; 15746664 AB - Cerebral palsy (CP) is associated with childhood spasticity, seizures, and paralysis. Oligodendrocyte damage resulting in periventicular leukomalacia (PVL) in the developing brain has been implicated. Animal models of CP have used prenatal hypoxia and infection with histopathology of PVL as the end point. To evaluate whether this histologic end point is associated with a CP phenotype, we reproduced a lipopolysaccharide (LPS) model for PVL, 1 and evaluated developmental, behavioral, and motor outcomes. On gestational day 15, Fischer 344 rats were intracervically injected with .1 mg/kg LPS (n = 5) or saline (n = 4). After delivery, evaluation for developmental milestones was performed on days 1 to 21 (LPS = 45; control = 30 pups). Males were also tested at 2.5 months using open-field, rotarod, and anxiety tests. On day 21, 2 pups/litter were perfused for immunohistochemistry, and stained with 2 oligodendrocyte antibodies: 2', d'-cyclic nucleotide phosphodiesterase (CNP), and myelin proteolipid protein (PLP) with relative densities of staining assessed using NIH Image software. Statistical analysis included Mann-Whitney U and analysis of variance (ANOVA). LPS pups demonstrated decreased CNP (P = .04) and PLP (P = .06) staining, replicating the model. There was no difference seen in neonatal weight, righting, negative geotaxis, cliff aversion, rooting, forelimb grasp, audio startle, air righting, eye opening, and activity. Surprisingly, LPS-exposed neonatal rats mastered forelimb placement (P < .01) and surface righting (P = .02) earlier than control rats. There were no differences between adult groups in open field distance traveled (P = .8), open-field locomotion time (P = .6), rotarod (P = .6), or anxiety (P = .7). Histologic evidence of white matter damage can be replicated using an LPS model for intrauterine inflammation. Significant phenotypic differences consistent with the motor and cognitive damage sequelae of such lesions (ie, CP) were not demonstrated. When evaluating animal models, it is important to assess not only biochemical markers for human disease, but also clinically relevant phenotypes. JF - American journal of obstetrics and gynecology AU - Poggi, Sarah H AU - Park, Jane AU - Toso, Laura AU - Abebe, Daniel AU - Roberson, Robin AU - Woodard, Jade E AU - Spong, Catherine Y AD - Unit on Perinatal and Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. sarah.poggi@inova.com Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 727 EP - 733 VL - 192 IS - 3 SN - 0002-9378, 0002-9378 KW - Lipopolysaccharides KW - 0 KW - Myelin Proteolipid Protein KW - 2',3'-Cyclic-Nucleotide Phosphodiesterases KW - EC 3.1.4.- KW - Abridged Index Medicus KW - Index Medicus KW - Rats KW - Myelin Proteolipid Protein -- analysis KW - Animals KW - Rats, Inbred F344 KW - 2',3'-Cyclic-Nucleotide Phosphodiesterases -- analysis KW - Humans KW - Infant, Newborn KW - Disease Models, Animal KW - Immunohistochemistry KW - Male KW - Leukomalacia, Periventricular -- physiopathology KW - Cognition -- physiology KW - Lipopolysaccharides -- administration & dosage KW - Cerebral Palsy -- physiopathology KW - Motor Activity -- physiology KW - Leukomalacia, Periventricular -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67487182?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+obstetrics+and+gynecology&rft.atitle=No+phenotype+associated+with+established+lipopolysaccharide+model+for+cerebral+palsy.&rft.au=Poggi%2C+Sarah+H%3BPark%2C+Jane%3BToso%2C+Laura%3BAbebe%2C+Daniel%3BRoberson%2C+Robin%3BWoodard%2C+Jade+E%3BSpong%2C+Catherine+Y&rft.aulast=Poggi&rft.aufirst=Sarah&rft.date=2005-03-01&rft.volume=192&rft.issue=3&rft.spage=727&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=00029378&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-01 N1 - Date created - 2005-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The nitric oxide prodrug, V-PYRRO/NO, protects against cadmium toxicity and apoptosis at the cellular level. AN - 67483301; 15740985 AB - The liver is an important target tissue of cadmium. The compound O2-vinyl 1-(pyrrolidin-1-yl)diazen-1-ium-1,2 diolate (V-PYRRO/NO) is a liver-selective nitric oxide (NO) prodrug that is metabolized by hepatic P450 enzymes to release NO in hepatocytes. In vivo, V-PYRRO/NO can protect against the toxicity of various hepatotoxicants, including cadmium. Since NO is an effective vasodilator, whether this protective effect against cadmium toxicity is at the level of the hepatic vascular system or actually within the liver cells has not been defined. Thus, we studied the effects of V-PYRRO/NO pretreatment on cadmium-induced toxicity and apoptosis in cultured rat liver epithelial (TRL 1215) cells. Cells were pretreated with V-PYRRO/NO at 500 or 1000 microM for up to 24 h, then exposed to cadmium (as CdCl2) for additional 24 h and cytotoxicity was measured. Cadmium was significantly less cytotoxic in V-PYRRO/NO (1000 microM) pretreated cells (LC50=6.1+/-0.6 microM) compared to control cells (LC50=3.5+/-0.4 microM). TRL 1215 cells acted upon the prodrug to release NO, producing nitrite levels in the extracellular media after 24 h of exposure to 500 or 1000 microM V-PYRRO/NO measured at 87.0+/-4.2 and 324+/-14.8 microM, respectively, compared to basal levels of 7.70+/-0.46 microM. V-PYRRO/NO alone produced small increases in metallothionein (MT), a metal-binding protein associated with cadmium tolerance. However, V-PYRRO/NO pretreatment greatly enhanced cadmium induction of MT. V-PYRRO/NO pretreatment also markedly reduced apoptotic cell death induced by cadmium (5 microM), apparently by blocking cadmium-induced activation of the c-Jun N-terminal kinase (JNK) pathway. Thus, the prodrug, V-PYRRO/NO, protects against the adverse effects of cadmium directly within rat liver cells apparently through generation of NO and, at least in part, by facilitation of cadmium-induced MT synthesis. JF - Nitric oxide : biology and chemistry AU - Qu, Wei AU - Liu, Jie AU - Fuquay, Richard AU - Shimoda, Ryuya AU - Sakurai, Teruaki AU - Saavedra, Joseph E AU - Keefer, Larry K AU - Waalkes, Michael P AD - Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at the National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 114 EP - 120 VL - 12 IS - 2 SN - 1089-8603, 1089-8603 KW - Nitrites KW - 0 KW - O(2)-vinyl-1-(pyrrolidin-1-yl)diazen-1-ium-1,2-diolate KW - Prodrugs KW - Pyrrolidines KW - Nitric Oxide KW - 31C4KY9ESH KW - Metallothionein KW - 9038-94-2 KW - JNK Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Cadmium Chloride KW - J6K4F9V3BA KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Nitrites -- metabolism KW - Dose-Response Relationship, Drug KW - Prodrugs -- pharmacology KW - Metallothionein -- drug effects KW - Enzyme-Linked Immunosorbent Assay KW - Nitric Oxide -- biosynthesis KW - Metallothionein -- metabolism KW - JNK Mitogen-Activated Protein Kinases -- drug effects KW - Cell Line KW - JNK Mitogen-Activated Protein Kinases -- metabolism KW - Apoptosis -- physiology KW - Cadmium Chloride -- toxicity KW - Apoptosis -- drug effects KW - Pyrrolidines -- pharmacology KW - Cadmium Chloride -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67483301?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nitric+oxide+%3A+biology+and+chemistry&rft.atitle=The+nitric+oxide+prodrug%2C+V-PYRRO%2FNO%2C+protects+against+cadmium+toxicity+and+apoptosis+at+the+cellular+level.&rft.au=Qu%2C+Wei%3BLiu%2C+Jie%3BFuquay%2C+Richard%3BShimoda%2C+Ryuya%3BSakurai%2C+Teruaki%3BSaavedra%2C+Joseph+E%3BKeefer%2C+Larry+K%3BWaalkes%2C+Michael+P&rft.aulast=Qu&rft.aufirst=Wei&rft.date=2005-03-01&rft.volume=12&rft.issue=2&rft.spage=114&rft.isbn=&rft.btitle=&rft.title=Nitric+oxide+%3A+biology+and+chemistry&rft.issn=10898603&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-26 N1 - Date created - 2005-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - 3-hydroxymorphinan is neurotrophic to dopaminergic neurons and is also neuroprotective against LPS-induced neurotoxicity. AN - 67482729; 15596482 AB - The purpose of this study was to develop a novel therapy for Parkinson's disease (PD). We recently reported that dextromethorphan (DM), an active ingredient in a variety of widely used anticough remedies, protected dopaminergic neurons in rat primary mesencephalic neuron-glia cultures against lipopolysaccharide (LPS)-mediated degeneration and provided potent protection for dopaminergic neurons in a MPTP mouse model. The underlying mechanism for the protective effect of DM was attributed to its anti-inflammatory activity through inhibition of microglia activation. In an effort to develop more potent compounds for the treatment of PD, we have screened a series of analogs of DM, and 3-hydroxymorphinan (3-HM) emerged as a promising candidate for this purpose. Our study using primary mesencephalic neuron-glia cultures showed that 3-HM provided more potent neuroprotection against LPS-induced dopaminergic neurotoxicity than its parent compound. The higher potency of 3-HM was attributed to its neurotrophic effect in addition to the anti-inflammatory effect shared by both DM and 3-HM. First, we showed that 3-HM exerted potent neuroprotective and neurotrophic effects on dopaminergic neurons in rat primary mesencephalic neuron-glia cultures treated with LPS. The neurotrophic effect of 3-HM was glia-dependent since 3-HM failed to show any protective effect in the neuron-enriched cultures. We subsequently demonstrated that it was the astroglia, not the microglia, that contributed to the neurotrophic effect of 3-HM. This conclusion was based on the reconstitution studies, in which we added different percentages of microglia (10-20%) or astroglia (40-50%) back to the neuron-enriched cultures and found that 3-HM was neurotrophic after the addition of astroglia, but not microglia. Furthermore, 3-HM-treated astroglia-derived conditioned media exerted a significant neurotrophic effect on dopaminergic neurons. It appeared likely that 3-HM caused the release of neurotrophic factor(s) from astroglia, which in turn was responsible for the neurotrophic effect. Second, the anti-inflammatory mechanism was also important for the neuroprotective activity of 3-HM because the more microglia were added back to the neuron-enriched cultures, the more significant neuroprotective effect was observed. The anti-inflammatory mechanism of 3-HM was attributed to its inhibition of LPS-induced production of an array of pro-inflammatory and neurotoxic factors, including nitric oxide (NO), tumor necrosis factor alpha (TNF-alpha), prostaglandin E2 (PGE2) and reactive oxygen species (ROS). In conclusion, this study showed that 3-HM exerted potent neuroprotection by acting on two different targets: a neurotrophic effect mediated by astroglia and an anti-inflammatory effect mediated by the inhibition of microglial activation. 3-HM thus possesses these two important features necessary for an effective neuroprotective agent. In view of the well-documented very low toxicity of DM and its analogs, this report may provide an important new direction for the development of therapeutic interventions for inflammation-related diseases such as PD. JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology AU - Zhang, Wei AU - Qin, Liya AU - Wang, Tongguang AU - Wei, Sung-Jen AU - Gao, Hui-ming AU - Liu, Jie AU - Wilson, Belinda AU - Liu, Bin AU - Zhang, Wanqin AU - Kim, Hyoung-Chun AU - Hong, Jau-Shyong AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences/National Institutes of Health, Research Triangle Park, P.O. Box 12233, NC, USA. zhang11@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 395 EP - 397 VL - 19 IS - 3 KW - Anti-Inflammatory Agents KW - 0 KW - Culture Media, Conditioned KW - Lipopolysaccharides KW - Nerve Growth Factors KW - Neuroprotective Agents KW - Reactive Oxygen Species KW - Tumor Necrosis Factor-alpha KW - Nitric Oxide KW - 31C4KY9ESH KW - Dextromethorphan KW - 7355X3ROTS KW - norlevorphanol KW - IL94262N7K KW - Dinoprostone KW - K7Q1JQR04M KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Dinoprostone -- biosynthesis KW - Astrocytes -- drug effects KW - Astrocytes -- physiology KW - Tumor Necrosis Factor-alpha -- biosynthesis KW - Neuroglia -- drug effects KW - Nitric Oxide -- biosynthesis KW - Mesencephalon -- cytology KW - Neuroglia -- physiology KW - Parkinson Disease -- drug therapy KW - Rats KW - Microglia -- physiology KW - Microglia -- drug effects KW - Anti-Inflammatory Agents -- pharmacology KW - Dextromethorphan -- analogs & derivatives KW - Nerve Growth Factors -- pharmacology KW - Lipopolysaccharides -- antagonists & inhibitors KW - Neurons -- drug effects KW - Dextromethorphan -- pharmacology KW - Neurons -- physiology KW - Dopamine -- physiology KW - Lipopolysaccharides -- toxicity KW - Neuroprotective Agents -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67482729?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.atitle=3-hydroxymorphinan+is+neurotrophic+to+dopaminergic+neurons+and+is+also+neuroprotective+against+LPS-induced+neurotoxicity.&rft.au=Zhang%2C+Wei%3BQin%2C+Liya%3BWang%2C+Tongguang%3BWei%2C+Sung-Jen%3BGao%2C+Hui-ming%3BLiu%2C+Jie%3BWilson%2C+Belinda%3BLiu%2C+Bin%3BZhang%2C+Wanqin%3BKim%2C+Hyoung-Chun%3BHong%2C+Jau-Shyong&rft.aulast=Zhang&rft.aufirst=Wei&rft.date=2005-03-01&rft.volume=19&rft.issue=3&rft.spage=395&rft.isbn=&rft.btitle=&rft.title=FASEB+journal+%3A+official+publication+of+the+Federation+of+American+Societies+for+Experimental+Biology&rft.issn=1530-6860&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-25 N1 - Date created - 2005-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Vitamin A supplementation for extremely low birth weight infants: outcome at 18 to 22 months. AN - 67482339; 15713907 AB - A National Institute of Child Health and Human Development Neonatal Research Network randomized trial showed that vitamin A supplementation reduced bronchopulmonary dysplasia (O2 at 36 weeks' postmenstrual age) or death in extremely low birth weight (ELBW) neonates (relative risk [RR]: 0.89). As with postnatal steroids or other interventions, it is important to ensure that there are no longer-term adverse effects that outweigh neonatal benefits. To determine if vitamin A supplementation in ELBW infants during the first month after birth affects survival without neurodevelopmental impairment at a corrected age of 18 to 22 months. Infants enrolled in the National Institute of Child Health and Human Development vitamin A trial were evaluated at 18 to 22 months by carefully standardized assessments: Bayley Mental Index (MDI) and Psychomotor Index (PDI), visual and hearing screens, and physical examination for cerebral palsy (CP). The medical history was also obtained. Neurodevelopmental impairment (NDI) was predefined as > or =1 of MDI <70, PDI <70, CP, blind in both eyes, or hearing aids in both ears. Of 807 enrolled infants, 133 died before and 16 died after discharge. Five hundred seventy-nine (88%) of the 658 remaining infants were followed up. The primary outcome of NDI or death could be determined for 687 of 807 randomized infants (85%). Baseline characteristics and predischarge and postdischarge mortality were comparable in both study groups. NDI or death by 18 to 22 months occurred in 190 of 345 (55%) infants in the vitamin A group and in 204 of 342 (60%) of the control group (RR: 0.94; 95% confidence interval: 0.80-1.07). RRs for low MDI, low PDI, and CP were also <1.0. We found no evidence that neonatal vitamin A supplementation reduces hospitalizations or pulmonary problems after discharge. Vitamin A supplementation for ELBW infants reduces bronchopulmonary dysplasia without increasing mortality or neurodevelopmental impairment at 18 to 22 months. However, this study was not powered to evaluate small magnitudes of change in long-term outcomes. JF - Pediatrics AU - Ambalavanan, Namasivayam AU - Tyson, Jon E AU - Kennedy, Kathleen A AU - Hansen, Nellie I AU - Vohr, Betty R AU - Wright, Linda L AU - Carlo, Waldemar A AU - National Institute of Child Health and Human Development Neonatal Research Network AD - Department of Pediatrics, University of Alabama, Birmingham, Alabama 35249, USA. ambal@uab.edu ; National Institute of Child Health and Human Development Neonatal Research Network Y1 - 2005/03// PY - 2005 DA - March 2005 SP - e249 EP - e254 VL - 115 IS - 3 KW - Vitamin A KW - 11103-57-4 KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Infant, Newborn KW - Infant Mortality KW - Follow-Up Studies KW - Neuropsychological Tests KW - Cerebral Palsy -- chemically induced KW - Male KW - Female KW - Vitamin A -- therapeutic use KW - Infant, Very Low Birth Weight KW - Developmental Disabilities -- chemically induced KW - Vitamin A -- adverse effects KW - Bronchopulmonary Dysplasia -- prevention & control KW - Child Development -- drug effects KW - Developmental Disabilities -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67482339?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatrics&rft.atitle=Vitamin+A+supplementation+for+extremely+low+birth+weight+infants%3A+outcome+at+18+to+22+months.&rft.au=Ambalavanan%2C+Namasivayam%3BTyson%2C+Jon+E%3BKennedy%2C+Kathleen+A%3BHansen%2C+Nellie+I%3BVohr%2C+Betty+R%3BWright%2C+Linda+L%3BCarlo%2C+Waldemar+A%3BNational+Institute+of+Child+Health+and+Human+Development+Neonatal+Research+Network&rft.aulast=Ambalavanan&rft.aufirst=Namasivayam&rft.date=2005-03-01&rft.volume=115&rft.issue=3&rft.spage=e249&rft.isbn=&rft.btitle=&rft.title=Pediatrics&rft.issn=1098-4275&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-17 N1 - Date created - 2005-03-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Occupational exposure to carbofuran and the incidence of cancer in the Agricultural Health Study. AN - 67477836; 15743716 AB - Carbofuran is a carbamate insecticide registered for use on a variety of food crops including corn, alfalfa, rice, and tobacco. An estimated 5 million pounds of carbofuran is used annually in the United States, and 45% of urban African-American women have detectable levels of carbofuran in their plasma. Nitrosated carbofuran has demonstrated mutagenic properties. We examined exposure to carbofuran and several tumor sites among 49,877 licensed pesticide applicators from Iowa and North Carolina enrolled in the Agricultural Health Study. We obtained information regarding years of use, frequency of use in an average year, and when use began for 22 pesticides using self-administered questionnaires. Poisson regression was used to calculate rate ratios (RR) and 95% confidence intervals (CIs) adjusting for potential confounders. Lung cancer risk was 3-fold higher for those with > 109 days of lifetime exposure to carbofuran (RR = 3.05; 95% CI, 0.94-9.87) compared with those with < 9 lifetime exposure days, with a significant dose-response trend for both days of use per year and total years of use. However, carbofuran use was not associated with lung cancer risk when nonexposed persons were used as the referent. In addition, carbofuran exposure was not associated with any other cancer site examined. Although carbamate pesticides are suspected human carcinogens, these results should be interpreted cautiously because there was no a priori hypothesis specifically linking carbofuran to lung cancer. JF - Environmental health perspectives AU - Bonner, Matthew R AU - Lee, Won Jin AU - Sandler, Dale P AU - Hoppin, Jane A AU - Dosemeci, Mustafa AU - Alavanja, Michael C R AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland, USA. bonnerm@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 285 EP - 289 VL - 113 IS - 3 SN - 0091-6765, 0091-6765 KW - Insecticides KW - 0 KW - Carbofuran KW - SKF77S6Y67 KW - Index Medicus KW - Agriculture KW - Epidemiologic Studies KW - Humans KW - Adult KW - Incidence KW - Aged KW - Middle Aged KW - North Carolina -- epidemiology KW - Male KW - Iowa -- epidemiology KW - Female KW - Occupational Exposure KW - Insecticides -- poisoning KW - Neoplasms -- epidemiology KW - Carbofuran -- poisoning KW - Neoplasms -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67477836?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Occupational+exposure+to+carbofuran+and+the+incidence+of+cancer+in+the+Agricultural+Health+Study.&rft.au=Bonner%2C+Matthew+R%3BLee%2C+Won+Jin%3BSandler%2C+Dale+P%3BHoppin%2C+Jane+A%3BDosemeci%2C+Mustafa%3BAlavanja%2C+Michael+C+R&rft.aulast=Bonner&rft.aufirst=Matthew&rft.date=2005-03-01&rft.volume=113&rft.issue=3&rft.spage=285&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-18 N1 - Date created - 2005-03-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mutat Res. 1983 Mar;116(3-4):185-216 [6339892] J Environ Pathol Toxicol. 1980 Nov;4(5-6):53-63 [7217860] IARC Sci Publ. 1987;(82):1-406 [3329634] J Occup Med. 1990 Oct;32(10):996-1002 [2262830] Cancer Causes Control. 1993 Sep;4(5):449-54 [8218877] Cancer Causes Control. 1994 Jul;5(4):310-8 [8080942] J Toxicol Environ Health. 1994 Dec;43(4):383-418 [7990167] Environ Health Perspect. 1996 Apr;104(4):362-9 [8732939] Am J Ind Med. 1996 Nov;30(5):601-9 [8909609] Mutagenesis. 1998 Mar;13(2):157-66 [9568589] Int J Epidemiol. 1999 Jun;28(3):365-74 [10405835] Environ Res. 1986 Dec;41(2):633-45 [3490967] Toxicology. 2000 Feb 7;143(1):1-118 [10675783] Mutat Res. 2000 Feb 16;465(1-2):123-9 [10708977] J Occup Environ Med. 2001 Jul;43(7):641-9 [11464396] Cancer Epidemiol Biomarkers Prev. 2001 Nov;10(11):1155-63 [11700263] Toxicology. 2001 Dec 14;169(2):153-61 [11718956] Epidemiology. 2002 Jan;13(1):94-9 [11805592] Ann Occup Hyg. 2002 Mar;46(2):245-60 [12074034] Environ Health Perspect. 2003 May;111(5):749-56 [12727605] J Allergy Clin Immunol. 2003 Jul;112(1):219-20 [12847509] Ann Agric Environ Med. 2003;10(2):229-32 [14677917] Arch Environ Health. 2003 May;58(5):316-7; author reply 317 [14738278] J Occup Med. 1970 Jan;12(1):16-9 [5410616] Toxicol Appl Pharmacol. 1975 Jun;32(3):587-602 [50651] Int J Cancer. 1976 Jun 15;17(6):742-7 [988851] Neoplasma. 1977;24(1):119-22 [576495] Erratum In: Environ Health Perspect. 2005 May;113(5):A297 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Invasive human magnetic resonance imaging: feasibility during revascularization in a combined XMR suite. AN - 67477527; 15736247 AB - We tested the feasibility and safety of invasive magnetic resonance imaging (MRI) during peripheral angioplasty. Real-time MRI can image soft tissue and may potentially guide therapeutic procedures without ionizing radiation or nephrotoxic contrast. MRI-guided diagnostic catheterization has been described recently, but safe and conspicuous catheter devices are not widely available. An active guidewire, which serves as an MRI receiver antenna, might be useful to guide catheterization or even to image atheroma. We describe a combined interventional suite offering both X-ray fluoroscopy and real-time MRI. We used a 0.030'' active guidewire receiver coil for invasive MRI after X-ray lesion traversal in patients undergoing percutaneous iliofemoral artery revascularization. Intravascular MRI was compared with noninvasive MRI, X-ray angiography, and intravascular ultrasound (IVUS). Seven eligible patients consented to participate, but three were excluded because of lengthy revascularization procedures. Four remaining patients safely underwent combined X-ray fluoroscopy and real-time magnetic resonance imaging (XMR) transport, continuous monitoring, and all imaging modalities. There was no device dislodgment, contamination or evidence of heating. The intravascular MRI coil was well visualized except at the tip, but did not provide superior mural imaging compared with IVUS. Therefore, because an adequate safety and workflow experience was obtained, enrollment was terminated after only four subjects. Invasive MRI is feasible and apparently safe during peripheral angioplasty. Patients can safely be transported and monitored in an XMR interventional suite. An active quarter-wavelength guidewire coil does not provide superior imaging compared with IVUS, but provides satisfactory guidewire visualization. These tools may prove useful for advanced therapeutic procedures in the future. JF - Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions AU - Dick, Alexander J AU - Raman, Venkatesh K AU - Raval, Amish N AU - Guttman, Michael A AU - Thompson, Richard B AU - Ozturk, Cengizhan AU - Peters, Dana C AU - Stine, Annette M AU - Wright, Victor J AU - Schenke, William H AU - Lederman, Robert J AD - Cardiovascular Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 265 EP - 274 VL - 64 IS - 3 SN - 1522-1946, 1522-1946 KW - Index Medicus KW - Ultrasonography, Interventional KW - Iliac Artery -- diagnostic imaging KW - Feasibility Studies KW - Angiography KW - Iliac Artery -- pathology KW - Humans KW - Femoral Artery -- diagnostic imaging KW - Equipment Safety KW - Aged KW - Intraoperative Period KW - Femoral Artery -- pathology KW - Magnetic Resonance Angiography -- instrumentation KW - Radiology Department, Hospital KW - Operating Rooms KW - Intermittent Claudication -- diagnosis KW - Angioplasty, Balloon KW - Intermittent Claudication -- therapy KW - Magnetic Resonance Angiography -- standards UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67477527?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Catheterization+and+cardiovascular+interventions+%3A+official+journal+of+the+Society+for+Cardiac+Angiography+%26+Interventions&rft.atitle=Invasive+human+magnetic+resonance+imaging%3A+feasibility+during+revascularization+in+a+combined+XMR+suite.&rft.au=Dick%2C+Alexander+J%3BRaman%2C+Venkatesh+K%3BRaval%2C+Amish+N%3BGuttman%2C+Michael+A%3BThompson%2C+Richard+B%3BOzturk%2C+Cengizhan%3BPeters%2C+Dana+C%3BStine%2C+Annette+M%3BWright%2C+Victor+J%3BSchenke%2C+William+H%3BLederman%2C+Robert+J&rft.aulast=Dick&rft.aufirst=Alexander&rft.date=2005-03-01&rft.volume=64&rft.issue=3&rft.spage=265&rft.isbn=&rft.btitle=&rft.title=Catheterization+and+cardiovascular+interventions+%3A+official+journal+of+the+Society+for+Cardiac+Angiography+%26+Interventions&rft.issn=15221946&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-07 N1 - Date created - 2005-03-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Cardiovasc Magn Reson. 2002;4(4):431-42 [12549231] Phys Med Biol. 2003 Jul 21;48(14):R37-64 [12894968] J Vasc Interv Radiol. 2003 Oct;14(10):1317-27 [14551280] Circulation. 2003 Dec 9;108(23):2899-904 [14656911] Lancet. 2003 Dec 6;362(9399):1877-82 [14667742] Magn Reson Med. 2004 Apr;51(4):668-75 [15065238] J Magn Reson Imaging. 1998 Jan-Feb;8(1):220-5 [9500284] J Magn Reson Imaging. 1998 Jan-Feb;8(1):226-34 [9500285] J Magn Reson Imaging. 1998 Nov-Dec;8(6):1338-42 [9848749] Radiology. 1999 Sep;212(3):876-84 [10478260] J Am Coll Cardiol. 2005 Jun 21;45(12):2069-77 [15963411] Magn Reson Med. 2003 Feb;49(2):216-22 [12541240] Circulation. 2003 Jan 7;107(1):132-8 [12515755] Circulation. 2002 Jul 23;106(4):511-5 [12135954] Med Sci Monit. 2002 Jul;8(7):MT113-7 [12118208] Circulation. 2002 Mar 19;105(11):1282-4 [11901036] Circulation. 2002 Feb 19;105(7):874-9 [11854130] Magn Reson Med. 2002 Jan;47(1):187-93 [11754458] J Magn Reson Imaging. 2001 Jul;14(1):56-62 [11436215] J Magn Reson Imaging. 2001 Jan;13(1):105-14 [11169811] Int J Epidemiol. 2000 Jun;29(3):424-8 [10869313] J Magn Reson Imaging. 2003 May;17(5):615-9 [12720273] Magn Reson Med. 2003 Aug;50(2):383-90 [12876715] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prediction and prevention of transplant-related mortality from pulmonary causes after total body irradiation and allogeneic stem cell transplantation. AN - 67476492; 15744241 AB - Between July 1997 and August 2004, 146 consecutive patients with hematologic malignancies received a T cell-depleted peripheral blood stem cell transplant from an HLA-identical sibling by using total body irradiation (TBI) and cyclophosphamide conditioning regimens. Eighty-five patients received 13.6 Gy of TBI with no lung shielding, and 61 received lung shielding (total lung dose, 6-12 Gy). Ninety-four patients (65.5%) had standard-risk disease; the remainder had more advanced disease or unfavorable diagnoses. Of the 21 transplant-related deaths, 14 were from pulmonary causes (10 idiopathic pulmonary syndromes and 4 from infection) that occurred at a median of 90 days (range, 23-238 days) after transplantation. Independent risk factors for pulmonary transplant-related mortality (PTRM) were pretransplantation diffusion capacity for carbon monoxide (relative risk, 5.7 for diffusion capacity for carbon monoxide <85%), smoking (relative risk, 5.0), and CD34 cell dose (relative risk, 9.4 for a CD34 dose of <5 x 10(6) cells per kilogram). Patients receiving lung shielding had significantly lower PTRM (3.3% versus 14.1%; P = .02) and better overall survival (70% +/- 6% versus 52% +/- 5%; P = .04), but lung shielding was not a significant independent factor for determining PTRM. These results suggest that pulmonary mortality after TBI-based preparative regimens is predictable and that higher CD34 cell doses can reduce the risk. JF - Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation AU - Savani, Bipin N AU - Montero, Aldemar AU - Wu, Colin AU - Nlonda, Nene AU - Read, Elizabeth AU - Dunbar, Cynthia AU - Childs, Richard AU - Solomon, Scott AU - Barrett, A John AD - Stem Cell Allotransplant Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 223 EP - 230 VL - 11 IS - 3 SN - 1083-8791, 1083-8791 KW - Antigens, CD34 KW - 0 KW - Cyclophosphamide KW - 8N3DW7272P KW - Index Medicus KW - Humans KW - Retrospective Studies KW - Pneumonia, Pneumococcal KW - Prognosis KW - Child KW - Transplantation, Homologous KW - Transplantation Conditioning -- adverse effects KW - Cyclophosphamide -- therapeutic use KW - Risk Factors KW - Transplantation Conditioning -- methods KW - Adult KW - Follow-Up Studies KW - Middle Aged KW - Adolescent KW - Female KW - Male KW - Lung Diseases -- etiology KW - Whole-Body Irradiation -- mortality KW - Whole-Body Irradiation -- methods KW - Hematopoietic Stem Cell Transplantation -- mortality KW - Whole-Body Irradiation -- adverse effects KW - Lung Diseases -- mortality KW - Lung Diseases -- prevention & control KW - Hematopoietic Stem Cell Transplantation -- methods KW - Hematopoietic Stem Cell Transplantation -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67476492?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biology+of+blood+and+marrow+transplantation+%3A+journal+of+the+American+Society+for+Blood+and+Marrow+Transplantation&rft.atitle=Prediction+and+prevention+of+transplant-related+mortality+from+pulmonary+causes+after+total+body+irradiation+and+allogeneic+stem+cell+transplantation.&rft.au=Savani%2C+Bipin+N%3BMontero%2C+Aldemar%3BWu%2C+Colin%3BNlonda%2C+Nene%3BRead%2C+Elizabeth%3BDunbar%2C+Cynthia%3BChilds%2C+Richard%3BSolomon%2C+Scott%3BBarrett%2C+A+John&rft.aulast=Savani&rft.aufirst=Bipin&rft.date=2005-03-01&rft.volume=11&rft.issue=3&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=Biology+of+blood+and+marrow+transplantation+%3A+journal+of+the+American+Society+for+Blood+and+Marrow+Transplantation&rft.issn=10838791&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-22 N1 - Date created - 2005-03-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Integrase inhibitors to treat HIV/AIDS. AN - 67476071; 15729361 AB - HIV integrase is a rational target for treating HIV infection and preventing AIDS. It took approximately 12 years to develop clinically usable inhibitors of integrase, and Phase I clinical trials of integrase inhibitors have just begun. This review focuses on the molecular basis and rationale for developing integrase inhibitors. The main classes of lead compounds are also described, as well as the concept of interfacial inhibitors of protein-nucleic-acid interactions that might apply to the clinically used strand-transfer inhibitors. JF - Nature reviews. Drug discovery AU - Pommier, Yves AU - Johnson, Allison A AU - Marchand, Christophe AD - Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 236 EP - 248 VL - 4 IS - 3 SN - 1474-1776, 1474-1776 KW - HIV Integrase Inhibitors KW - 0 KW - Index Medicus KW - United States KW - Antiretroviral Therapy, Highly Active -- adverse effects KW - United States Food and Drug Administration KW - Africa South of the Sahara -- epidemiology KW - Humans KW - Drug Approval KW - Mutation KW - Structure-Activity Relationship KW - HIV Integrase Inhibitors -- therapeutic use KW - HIV -- physiology KW - Virus Replication -- drug effects KW - HIV Integrase Inhibitors -- chemistry KW - HIV Infections -- drug therapy KW - HIV Infections -- mortality KW - Virus Replication -- physiology KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67476071?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+reviews.+Drug+discovery&rft.atitle=Integrase+inhibitors+to+treat+HIV%2FAIDS.&rft.au=Pommier%2C+Yves%3BJohnson%2C+Allison+A%3BMarchand%2C+Christophe&rft.aulast=Pommier&rft.aufirst=Yves&rft.date=2005-03-01&rft.volume=4&rft.issue=3&rft.spage=236&rft.isbn=&rft.btitle=&rft.title=Nature+reviews.+Drug+discovery&rft.issn=14741776&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-01 N1 - Date created - 2005-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The promise of genetically engineered mice for cancer prevention studies. AN - 67475504; 15738982 AB - Sophisticated genetic technologies have led to the development of mouse models of human cancers that recapitulate important features of human oncogenesis. Many of these genetically engineered mouse models promise to be very relevant and relatively rapid systems for determining the efficacy of chemopreventive agents and their mechanisms of action. The validation of such models for chemoprevention will help the selection of appropriate agents for large-scale clinical trials and allow the testing of combination therapies. JF - Nature reviews. Cancer AU - Green, Jeffrey E AU - Hudson, Tamaro AD - Transgenic Oncogenesis Group, Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, Besthesda, MD 20892, USA. jegreen@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 184 EP - 198 VL - 5 IS - 3 SN - 1474-175X, 1474-175X KW - Antineoplastic Agents KW - 0 KW - Index Medicus KW - Animals KW - Colonic Neoplasms -- genetics KW - Humans KW - Colonic Neoplasms -- veterinary KW - Mammary Neoplasms, Experimental -- genetics KW - Mice KW - Antineoplastic Agents -- pharmacology KW - Disease Models, Animal KW - Neoplasms -- prevention & control KW - Chemoprevention KW - Animals, Genetically Modified KW - Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67475504?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+reviews.+Cancer&rft.atitle=The+promise+of+genetically+engineered+mice+for+cancer+prevention+studies.&rft.au=Green%2C+Jeffrey+E%3BHudson%2C+Tamaro&rft.aulast=Green&rft.aufirst=Jeffrey&rft.date=2005-03-01&rft.volume=5&rft.issue=3&rft.spage=184&rft.isbn=&rft.btitle=&rft.title=Nature+reviews.+Cancer&rft.issn=1474175X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-22 N1 - Date created - 2005-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - New science-based endpoints to accelerate oncology drug development. AN - 67473871; 15737552 AB - Although several new oncology drugs have reached the market, more than 80% of drugs for all indications entering clinical development do not get marketing approval, with many failing late in development often in Phase III trials, because of unexpected safety issues or difficulty determining efficacy, including confounded outcomes. These factors contribute to the high costs of oncology drug development and clearly show the need for faster, more cost-effective strategies for evaluating oncology drugs and better definition of patients who will benefit from treatment. Remarkable advances in the understanding of neoplastic progression at the cellular and molecular levels have spurred the discovery of molecularly targeted drugs. This progress along with advances in imaging and bioassay technologies are the basis for describing and evaluating new biomarker endpoints as well as for defining other biomarkers for identifying patient populations, potential toxicity, and providing evidence of drug effect and efficacy. Definitions and classifications of these biomarkers for use in oncology drug development are presented in this paper. Science-based and practical criteria for validating biomarkers have been developed including considerations of mechanistic plausibility, available methods and technology, and clinical feasibility. New promising tools for measuring biomarkers have also been developed and are based on genomics and proteomics, direct visualisation by microscopy (e.g., confocal microscopy and computer-assisted image analysis of cellular features), nanotechnologies, and direct and remote imaging (e.g., fluorescence endoscopy and anatomical, functional and molecular imaging techniques). The identification and evaluation of potential surrogate endpoints and other biomarkers require access to and analysis of large amounts of data, new technologies and extensive research resources. Further, there is a requirement for a convergence of research, regulatory and drug developer thinking - an effort that will not be accomplished by individual scientists or research institutions. Research collaborations are needed to foster development of these new endpoints and other biomarkers and, in the United States (US), include ongoing efforts among the Food and Drug Administration (FDA), National Cancer Institute (NCI), academia, and industry. JF - European journal of cancer (Oxford, England : 1990) AU - Kelloff, Gary J AU - Sigman, Caroline C AD - Division of Cancer Treatment and Diagnosis, Cancer Imaging Program, National Cancer Institute, Executive Plaza North Room 6038, 9000 Rockville Pike, Bethesda, MD 20892, USA. kelloffg@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 491 EP - 501 VL - 41 IS - 4 SN - 0959-8049, 0959-8049 KW - Antineoplastic Agents KW - 0 KW - Biomarkers, Tumor KW - Index Medicus KW - Costs and Cost Analysis KW - Humans KW - Prognosis KW - Biomarkers, Tumor -- analysis KW - Forecasting KW - Neoplasms -- drug therapy KW - Antineoplastic Agents -- economics KW - Antineoplastic Agents -- therapeutic use KW - Drug Design UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67473871?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=European+journal+of+cancer+%28Oxford%2C+England+%3A+1990%29&rft.atitle=New+science-based+endpoints+to+accelerate+oncology+drug+development.&rft.au=Kelloff%2C+Gary+J%3BSigman%2C+Caroline+C&rft.aulast=Kelloff&rft.aufirst=Gary&rft.date=2005-03-01&rft.volume=41&rft.issue=4&rft.spage=491&rft.isbn=&rft.btitle=&rft.title=European+journal+of+cancer+%28Oxford%2C+England+%3A+1990%29&rft.issn=09598049&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Association of a functional polymorphism in the serotonin transporter gene with abnormal emotional processing in ecstasy users. AN - 67473793; 15741482 AB - The long-term effects of the use of 3,4-methylenedioxymethamphetamine (MDMA, or Ecstasy) in humans are controversial and unclear. The authors' goal was to assess the contribution of a functional polymorphism in the gene encoding serotonin transporter to changes in emotional processing following chronic Ecstasy use. They investigated Beck Depression Inventory scores and performance on the Affective Go/No-Go test, a computerized neuropsychological test sensitive to emotional processing, in Ecstasy users and comparison subjects, stratifying the results by serotonin transporter genotype. Ecstasy use was associated with higher Beck Depression Inventory score and abnormalities in the Affective Go/No-Go test in individuals with the ss and ls genotype but not those with the ll genotype. Ecstasy users carrying the s allele, but not comparison subjects carrying the s allele, showed abnormal emotional processing. On the basis of a comparison with acute tryptophan depletion, the authors hypothesize that chronic Ecstasy use may cause long-term changes to the serotonin system, and that Ecstasy users carrying the s allele may be at particular risk for emotional dysfunction. JF - The American journal of psychiatry AU - Roiser, Jonathan P AU - Cook, Lynnette J AU - Cooper, Jason D AU - Rubinsztein, David C AU - Sahakian, Barbara J AD - Department of Psychiatry, University of Cambridge School of Clinical Medicine, Addenbrooke,s Hospital, Cambridge CB2 2QQ, UK. roiserj@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 609 EP - 612 VL - 162 IS - 3 SN - 0002-953X, 0002-953X KW - Membrane Glycoproteins KW - 0 KW - Membrane Transport Proteins KW - Nerve Tissue Proteins KW - SLC6A4 protein, human KW - Serotonin Plasma Membrane Transport Proteins KW - Tryptophan KW - 8DUH1N11BX KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Abridged Index Medicus KW - Index Medicus KW - Genotype KW - Humans KW - Adult KW - Diagnosis, Dual (Psychiatry) KW - Depressive Disorder -- diagnosis KW - Depressive Disorder -- genetics KW - Personality Inventory KW - Tryptophan -- deficiency KW - Neuropsychological Tests KW - Male KW - Female KW - Substance-Related Disorders -- physiopathology KW - Affective Symptoms -- physiopathology KW - Nerve Tissue Proteins -- physiology KW - Affective Symptoms -- diagnosis KW - N-Methyl-3,4-methylenedioxyamphetamine -- adverse effects KW - Membrane Glycoproteins -- physiology KW - Polymorphism, Genetic -- genetics KW - Affective Symptoms -- genetics KW - Membrane Transport Proteins -- physiology KW - Nerve Tissue Proteins -- genetics KW - Substance-Related Disorders -- diagnosis KW - Polymorphism, Genetic -- physiology KW - Membrane Transport Proteins -- genetics KW - Substance-Related Disorders -- genetics KW - Membrane Glycoproteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67473793?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Association+of+a+functional+polymorphism+in+the+serotonin+transporter+gene+with+abnormal+emotional+processing+in+ecstasy+users.&rft.au=Roiser%2C+Jonathan+P%3BCook%2C+Lynnette+J%3BCooper%2C+Jason+D%3BRubinsztein%2C+David+C%3BSahakian%2C+Barbara+J&rft.aulast=Roiser&rft.aufirst=Jonathan&rft.date=2005-03-01&rft.volume=162&rft.issue=3&rft.spage=609&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-22 N1 - Date created - 2005-03-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Psychol Med. 1999 Nov;29(6):1307-21 [10616937] Neurology. 2000 Jul 25;55(2):294-6 [10908909] Psychopharmacology (Berl). 2001 Mar;154(3):319-26 [11351939] Neuroscience. 2002;110(1):41-8 [11882371] Lancet. 2001 Dec 1;358(9296):1864-9 [11741626] J Psychopharmacol. 2001 Sep;15(3):181-6 [11565625] Arch Gen Psychiatry. 2002 Jul;59(7):597-604 [12090812] J Psychopharmacol. 2002 Mar;16(1):103 [11949766] Arch Gen Psychiatry. 2002 Jul;59(7):613-20 [12090814] Psychopharmacology (Berl). 2002 Aug;163(1):42-53 [12185399] Pharmacol Rev. 2003 Sep;55(3):463-508 [12869661] Psychopharmacology (Berl). 2004 Jan;171(3):286-97 [12955284] Science. 1996 Nov 29;274(5292):1527-31 [8929413] Am J Med Genet. 1998 Feb 7;81(1):58-63 [9514589] Neuropsychopharmacology. 1998 Jul;19(1):26-35 [9608574] Mol Psychiatry. 1998 Nov;3(6):508-11 [9857976] Arch Gen Psychiatry. 1961 Jun;4:561-71 [13688369] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Electroporation loading of calcium-sensitive dyes into the CNS. AN - 67471573; 15509647 AB - Calcium imaging of neural network function has been limited by the extent of tissue labeled or the time taken for labeling. We now describe the use of electroporation-an established technique for transfecting cells with genes-to load neurons with calcium-sensitive dyes in the isolated spinal cord of the neonatal mouse in vitro. The dyes were injected subdurally, intravascularly, or into the central canal. This technique results in rapid and extensive labeling of neurons and their processes at all depths of the spinal cord, over a rostrocaudal extent determined by the position and size of the electrodes. Our results suggest that vascular distribution of the dye is involved in all three types of injections. Electroporation disrupts local reflex and network function only transiently (approximately 1 h), after which time they recover. We describe applications of the method to image activity of neuronal populations and individual neurons during antidromic, reflex, and locomotor-like behaviors. We show that these different motor behaviors are characterized by distinct patterns of activation among the labeled populations of cells. JF - Journal of neurophysiology AU - Bonnot, Agnès AU - Mentis, George Z AU - Skoch, Jesse AU - O'Donovan, Michael J AD - Laboratory of Neural Control, Section on Developmental Neurobiology, NINDS, National Institutes of Health, 35 Convent Dr., Rm. 3C1010, Bethesda, MD 20892, USA. bonnota@ninds.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 1793 EP - 1808 VL - 93 IS - 3 SN - 0022-3077, 0022-3077 KW - Fluorescent Dyes KW - 0 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Animals KW - Microinjections -- methods KW - Electric Stimulation -- methods KW - Staining and Labeling -- methods KW - Membrane Potentials -- physiology KW - Mice KW - Dose-Response Relationship, Radiation KW - Cell Survival KW - Diagnostic Imaging -- methods KW - Animals, Newborn KW - Calcium Signaling -- physiology KW - Electrophysiology -- methods KW - In Vitro Techniques KW - Time Factors KW - Calcium -- metabolism KW - Fluorescent Dyes -- metabolism KW - Neurons -- metabolism KW - Electroporation KW - Neurons -- classification KW - Spinal Cord -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67471573?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+neurophysiology&rft.atitle=Electroporation+loading+of+calcium-sensitive+dyes+into+the+CNS.&rft.au=Bonnot%2C+Agn%C3%A8s%3BMentis%2C+George+Z%3BSkoch%2C+Jesse%3BO%27Donovan%2C+Michael+J&rft.aulast=Bonnot&rft.aufirst=Agn%C3%A8s&rft.date=2005-03-01&rft.volume=93&rft.issue=3&rft.spage=1793&rft.isbn=&rft.btitle=&rft.title=Journal+of+neurophysiology&rft.issn=00223077&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-02 N1 - Date created - 2005-03-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Molecular epidemiology of HIV-1 subtypes in southern China. AN - 67465594; 15735457 AB - The driving forces of the HIV-1 epidemic in Guangxi, China were assessed by investigating virologic and epidemiologic data from a cohort of longitudinally followed injection drug users (IDUs) in Binyang and Pingxiang, major urban areas along 2 separate drug routes in the province. Sera and interview data were obtained in September and October of 2000. Sequence analysis of HIV-1 was performed on the gag-pol region (HXB2 nt 1850-3005) and C2 to V4 env (HXB2 nt 6704-7626). Sequence data demonstrated that 88% of the infections in Pingxiang were CRF01_AE, whereas 96% in Bin-yang were CRF08_BC. Three recently infected subjects in Pingxiang were infected with CRF08_BC, and 1 chronically infected subject had evidence of a recombinant virus. Intersubject distances were statistically greater for CRF01_AE-infected subjects than CRF08_BC-infected subjects for all regions except V4. The epidemic in Binyang is similar to previously described IDU-based epidemics, with a strong founder effect with little variation in V3. The epidemic in Pingxiang may have had multiple introductions of the CRF01_AE epidemic into the city and greater spread through sexual transmission, resulting in greater variation in V3 than typically seen in purely parenterally based epidemics. JF - Journal of acquired immune deficiency syndromes (1999) AU - Laeyendecker, Oliver AU - Zhang, Guang Wen AU - Quinn, Thomas C AU - Garten, Rebecca AU - Ray, Stuart C AU - Lai, Shenghan AU - Liu, Wei AU - Chen, Jie AU - Yu, Xiao-Fang AD - Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 356 EP - 362 VL - 38 IS - 3 SN - 1525-4135, 1525-4135 KW - DNA, Complementary KW - 0 KW - DNA, Viral KW - Fusion Proteins, gag-pol KW - Gene Products, env KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - HIV Protease KW - EC 3.4.23.- KW - Index Medicus KW - AIDS/HIV KW - Humans KW - Genes, pol KW - HIV Reverse Transcriptase -- genetics KW - Sequence Alignment KW - Molecular Epidemiology KW - Genes, env KW - Adult KW - Molecular Sequence Data KW - Urban Population KW - Genes, gag KW - Male KW - Gene Products, env -- chemistry KW - Phylogeny KW - HIV Protease -- genetics KW - Fusion Proteins, gag-pol -- genetics KW - Amino Acid Sequence KW - HIV Reverse Transcriptase -- chemistry KW - HIV Protease -- chemistry KW - Sequence Analysis, DNA KW - DNA, Viral -- chemistry KW - China -- epidemiology KW - DNA, Viral -- isolation & purification KW - Middle Aged KW - Substance Abuse, Intravenous -- complications KW - Female KW - HIV-1 -- genetics KW - HIV Infections -- virology KW - HIV-1 -- isolation & purification KW - HIV Infections -- epidemiology KW - HIV-1 -- classification UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67465594?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+acquired+immune+deficiency+syndromes+%281999%29&rft.atitle=Molecular+epidemiology+of+HIV-1+subtypes+in+southern+China.&rft.au=Laeyendecker%2C+Oliver%3BZhang%2C+Guang+Wen%3BQuinn%2C+Thomas+C%3BGarten%2C+Rebecca%3BRay%2C+Stuart+C%3BLai%2C+Shenghan%3BLiu%2C+Wei%3BChen%2C+Jie%3BYu%2C+Xiao-Fang&rft.aulast=Laeyendecker&rft.aufirst=Oliver&rft.date=2005-03-01&rft.volume=38&rft.issue=3&rft.spage=356&rft.isbn=&rft.btitle=&rft.title=Journal+of+acquired+immune+deficiency+syndromes+%281999%29&rft.issn=15254135&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-12 N1 - Date created - 2005-02-28 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - AY635760; GENBANK; AY635761; AY635762; AY635722; AY635721; AY635720; AY635726; AY635725; AY635724; AY635723; AY635729; AY635728; AY635727; AY635731; AY635730; AY635733; AY635732; AY635735; AY635734; AY635737; AY635736; AY635739; AY635738; AY635749; AY635745; AY635746; AY635747; AY635748; AY635741; AY635742; AY635743; AY635744; AY635740; AY635700; AY635708; AY635707; AY635706; AY635705; AY635704; AY635703; AY635702; AY635701; AY635758; AY635709; AY635691; AY635759; AY635692; AY635756; AY635757; AY635754; AY635755; AY635752; AY635753; AY635750; AY635751; AY635711; AY635710; AY635696; AY635717; AY635695; AY635716; AY635694; AY635719; AY635693; AY635718; AY635713; AY635712; AY635699; AY635715; AY635698; AY635714; AY635697 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Recovery from DSM-IV alcohol dependence: United States, 2001-2002. AN - 67465065; 15733237 AB - To investigate the prevalence and correlates of recovery from Diagnostic and Statistical Manual version IV (DSM-IV) alcohol dependence by examining the past-year status of individuals who met the criteria for prior-to-past-year (PPY) dependence. Cross-sectional, retrospective survey of a nationally representative sample of US adults 18 years of age and over (first wave of a planned longitudinal survey). This analysis is based on data from the 2001-02 National Epidemiologic Survey on Alcohol and Related Conditions (NESARC), in which data were collected in personal interviews conducted with one randomly selected adult in each sample household. A subset of the NESARC sample (total n = 43 093), consisting of 4422 US adults 18 years of age and over classified with PPY DSM-IV alcohol dependence, were evaluated with respect to their past-year recovery status: past-year dependence, partial remission, full remission, asymptomatic risk drinking, abstinent recovery (AR) and non-abstinent recovery (NR). Correlates of past-year status were examined in bivariate analyses and using multivariate logistic regression models. Of people classified with PPY alcohol dependence, 25.0% were still classified as dependent in the past year; 27.3% were classified as being in partial remission; 11.8% were asymptomatic risk drinkers who demonstrated a pattern of drinking that put them at risk of relapse; 17.7% were low-risk drinkers; and 18.2% were abstainers. Only 25.5% of people with PPY dependence ever received treatment. Being married was associated positively with the odds of both AR and NR, and ethanol intake was negatively associated with both. Severity of dependence increased the odds of AR but decreased the odds of NR. The odds of AR (but not NR) increased with age and female gender but were decreased by the presence of a personality disorder. Treatment history modified the effects of college attendance/graduation, age at onset and interval since onset on the odds of recovery. There is a substantial level of recovery from alcohol dependence. Information on factors associated with recovery may be useful in targeting appropriate treatment modalities. JF - Addiction (Abingdon, England) AU - Dawson, Deborah A AU - Grant, Bridget F AU - Stinson, Frederick S AU - Chou, Patricia S AU - Huang, Boji AU - Ruan, W June AD - Laboratory of Epidemiology and Biometry, Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA. ddawson@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 281 EP - 292 VL - 100 IS - 3 SN - 0965-2140, 0965-2140 KW - Index Medicus KW - Epidemiologic Methods KW - Humans KW - Aged KW - Recurrence KW - Remission, Spontaneous KW - Adult KW - Middle Aged KW - Adolescent KW - United States -- epidemiology KW - Temperance -- statistics & numerical data KW - Diagnostic and Statistical Manual of Mental Disorders KW - Female KW - Male KW - Alcoholism -- rehabilitation KW - Alcoholism -- epidemiology KW - Alcoholism -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67465065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=Recovery+from+DSM-IV+alcohol+dependence%3A+United+States%2C+2001-2002.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BStinson%2C+Frederick+S%3BChou%2C+Patricia+S%3BHuang%2C+Boji%3BRuan%2C+W+June&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-03-01&rft.volume=100&rft.issue=3&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-02-28 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Addiction. 2005 Mar;100(3):294; discussion 296-8 [15733239] Addiction. 2005 Mar;100(3):295-6; discussion 296-8 [15733241] Addiction. 2005 Mar;100(3):294-5; discussion 296-8 [15733240] Addiction. 2005 Mar;100(3):293; discussion 296-8 [15733238] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Retrospective analyses of pooled data from CREST I and CREST II trials for treatment of cocaine dependence. AN - 67460135; 15730353 AB - To analyze pooled data from the Cocaine Rapid Evaluation Screening Trial (CREST). Pooling data from these small pilot trials into four major drug classes permitted data exploration for treatment and covariate effects with increased sample size. Small pilot trials were conducted to screen fifteen medications as prospective treatments for cocaine dependence. Studies included a flexible 2-week to 4-week screening/baseline period followed by an 8-week randomized treatment condition. Participants were randomized equally to one of up to three active medications or placebo. Five Medications Development Research Units at the five academic centers of University of Cincinnati, New York University, University of Pennsylvania, University of California Los Angeles and Boston University. The pooled data set consisted of 357 total subjects. Standardized inclusion and exclusion criteria were employed in subject selection to enhance consistency of cocaine-dependent study participants across all sites (see reports on individual trials in this supplement for details). All participants provided at least two urine samples that were positive for cocaine metabolite during a two-week period prior to being randomized. All subjects in these trials, those randomized to placebo and active medications, received active treatment in the form of evidence-based cognitive behavioral therapy. Quantitative urine benzoylecgonine (BE), self-report of cocaine use, and total Brief Substance Craving Scale (BSCS) scores were compared between each class of medication and its matched-placebo group. Regression analysis of pooled data did not identify any statistically significant differences between treatment and matched-placebo for any of the four classes. Exploration of the effects of baseline covariates indicated that gender and African American status were associated significantly with outcome. Female gender was consistently associated with poorer outcomes for medication and placebo groups, while the direction of association between African American status and outcome differed by treatment groups. Retention was also examined: dropout rates may have been somewhat higher for placebo than treatment groups during the early active-treatment period. Classification trees were used to identify characteristics of subjects who were abstinent for at least two weeks during the eight-week trial; only 4.0% of females while 17.9% of males achieved this criterion. Results presented here may prove useful for planning future clinical trials for therapies targeting cocaine dependence. JF - Addiction (Abingdon, England) AU - Elkashef, Ahmed AU - Holmes, Tyson H AU - Bloch, Daniel A AU - Shoptaw, Steve AU - Kampman, Kyle AU - Reid, Malcolm S AU - Somoza, Eugene AU - Ciraulo, Domenic AU - Rotrosen, John AU - Leiderman, Deborah AU - Montgomery, Ann AU - Vocci, Frank AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 91 EP - 101 VL - 100 Suppl 1 SN - 0965-2140, 0965-2140 KW - Central Nervous System Agents KW - 0 KW - Dopamine Agonists KW - Placebos KW - Index Medicus KW - Randomized Controlled Trials as Topic KW - Logistic Models KW - Humans KW - Retrospective Studies KW - Clinical Trials as Topic KW - Pilot Projects KW - Male KW - Female KW - Dopamine Agonists -- therapeutic use KW - Cocaine-Related Disorders -- drug therapy KW - Central Nervous System Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67460135?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction+%28Abingdon%2C+England%29&rft.atitle=Retrospective+analyses+of+pooled+data+from+CREST+I+and+CREST+II+trials+for+treatment+of+cocaine+dependence.&rft.au=Elkashef%2C+Ahmed%3BHolmes%2C+Tyson+H%3BBloch%2C+Daniel+A%3BShoptaw%2C+Steve%3BKampman%2C+Kyle%3BReid%2C+Malcolm+S%3BSomoza%2C+Eugene%3BCiraulo%2C+Domenic%3BRotrosen%2C+John%3BLeiderman%2C+Deborah%3BMontgomery%2C+Ann%3BVocci%2C+Frank&rft.aulast=Elkashef&rft.aufirst=Ahmed&rft.date=2005-03-01&rft.volume=100+Suppl+1&rft.issue=&rft.spage=91&rft.isbn=&rft.btitle=&rft.title=Addiction+%28Abingdon%2C+England%29&rft.issn=09652140&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-02-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Polymorphisms in XPD and TP53 and mutation in human lung cancer. AN - 67455681; 15564288 AB - The pattern of somatic mutations in TP53 is distinct for particular cancers and carcinogenic exposures, providing clues to disease etiology, e.g. G:C-->T:A mutations in TP53 are more frequently observed in smoking-associated lung cancers. In order to investigate possible causes and mechanisms of lung cancer susceptibility differences, the TP53 gene was sequenced in a case-only study of lung cancers (206 men and 103 women). Our primary hypothesis was that the TP53 mutation spectrum is influenced by polymorphisms in genes involved in DNA repair and apoptosis. We observed a TP53 mutation frequency in exons 5-8 of 25%. Functional polymorphisms in XPD (Asp312Asn, rs1799793 and Lys751Gln, rs1052559), a protein required for nucleotide excision repair and with roles in p53-mediated apoptosis, were modestly associated with G:C-->T:A mutations in TP53 in lung tumors [Asp/Asn312 + Asn/Asn312 and/or Lys/Gln751 + Gln/Gln751 versus Asp/Asp312 + Lys/Lys751; odds ratio (OR) 2.73, 95% confidence interval (CI) 0.98-7.61], consistent with the role of this protein in repair of bulky carcinogen-DNA adducts. In addition, a TP53 polymorphism (Arg72Pro, rs1042522) with a known role in the efficiency of apoptosis was also associated with the presence of a TP53 mutation (Pro/Arg72 or Pro/Pro72 versus Arg/Arg72; OR 2.25, 95% CI 1.21-4.17) or a G:C-->T:A mutation in TP53 (Pro/Arg72 or Pro/Pro72 versus Arg/Arg72; OR 2.42, 95% CI 0.97-6.04). An interaction between the XPD variant alleles (Asn312 and Gln751) and the TP53 Pro72 allele was observed for TP53 mutations (any TP53 mutation P(int) = 0.027, G:C-->T:A TP53 mutation P(int) = 0.041). The statistical interaction observed in our study is consistent with the observed biological interaction for XPD and p53 in nucleotide excision repair and apoptosis. In conclusion, differences in TP53 mutation spectra in lung tumors are associated with several genetic factors and may reflect differences in lung cancer susceptibility and carcinogenesis. JF - Carcinogenesis AU - Mechanic, Leah E AU - Marrogi, Aizen J AU - Welsh, Judith A AU - Bowman, Elise D AU - Khan, Mohammed A AU - Enewold, Lindsey AU - Zheng, Yun-Ling AU - Chanock, Stephen AU - Shields, Peter G AU - Harris, Curtis C AD - Laboratory of Human Carcinogenesis, NCI Center for Cancer Research, 37 Convent Drive, Bethesda, MD 20892-4255, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 597 EP - 604 VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - DNA Primers KW - 0 KW - DNA-Binding Proteins KW - Transcription Factors KW - DNA Helicases KW - EC 3.6.4.- KW - Xeroderma Pigmentosum Group D Protein KW - EC 3.6.4.12 KW - ERCC2 protein, human KW - EC 5.99.- KW - Index Medicus KW - Base Sequence KW - Humans KW - Male KW - Female KW - Genes, p53 KW - Polymorphism, Genetic KW - DNA-Binding Proteins -- genetics KW - DNA Helicases -- genetics KW - Lung Neoplasms -- genetics KW - Transcription Factors -- genetics KW - Mutation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67455681?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Polymorphisms+in+XPD+and+TP53+and+mutation+in+human+lung+cancer.&rft.au=Mechanic%2C+Leah+E%3BMarrogi%2C+Aizen+J%3BWelsh%2C+Judith+A%3BBowman%2C+Elise+D%3BKhan%2C+Mohammed+A%3BEnewold%2C+Lindsey%3BZheng%2C+Yun-Ling%3BChanock%2C+Stephen%3BShields%2C+Peter+G%3BHarris%2C+Curtis+C&rft.aulast=Mechanic&rft.aufirst=Leah&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=597&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-13 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Meat intake, cooking-related mutagens and risk of colorectal adenoma in a sigmoidoscopy-based case-control study. AN - 67454846; 15579480 AB - Reported habits of red meat consumption, particularly red meat that has been cooked to the degree termed 'well-done', is a positive risk factor for colorectal cancer. Under high, pyrolytic temperatures, heterocyclic amines (HCA) and benzo[a]pyrene (BP) molecules can form inside and on the surface of red meat, respectively. These compounds are precursors that are metabolically converted to compounds known to act as mutagens and carcinogens in animal models, yet their role in human colorectal carcinogenesis remains to be clarified. We investigated whether intake of these compounds is associated with risk of colorectal adenoma in the context of a polyp-screening study conducted in Southern California. Using a database of individual HCAs and BP in meats of various types and subjected to specified methods and degrees of cooking, we estimated nanogram consumption of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 2-amino-3,4,8-trimethylimidazo[4,5-f] quinoxaline, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline and benzo[a]pyrene (BP). We observed a 6% increased risk of large (>1 cm) adenoma per 10 ng/day consumption of BP [OR = 1.06 (95% CI, 1.00-1.12), P (trend) = 0.04]. A major source of BP is red meat exposed to a naked flame, as occurs during the barbecuing process. Consistent with this finding an incremental increase of 10 g of barbecued red meat per day was associated with a 29% increased risk of large adenoma [OR = 1.29 (95% CI, 1.02-1.63), P (trend) = 0.04]. Individuals in the top quintile of barbecued red meat intake were at increased risk of large adenoma [OR = 1.90 (95% CI, 1.04-3.45)], compared with never consuming barbecued red meat. The consumption of oven-broiled red meat was inversely related to adenoma risk compared with non-consumers [OR = 0.49 (95% CI, 0.28-0.85)]. We did not identify any association with consumption of individual HCAs and colorectal adenoma risk. These results support the hypothesis that BP contributes to colorectal carcinogenesis. JF - Carcinogenesis AU - Gunter, Marc J AU - Probst-Hensch, Nicole M AU - Cortessis, Victoria K AU - Kulldorff, Martin AU - Haile, Robert W AU - Sinha, Rashmi AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, EPS, 6120 Executive Boulevard, Rockville, MD 20852, USA. gunterm@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 637 EP - 642 VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - Mutagens KW - 0 KW - Index Medicus KW - Humans KW - Case-Control Studies KW - Middle Aged KW - Male KW - Female KW - Meat KW - Sigmoidoscopy KW - Adenoma -- chemically induced KW - Mutagens -- toxicity KW - Colorectal Neoplasms -- etiology KW - Adenoma -- etiology KW - Diet KW - Colorectal Neoplasms -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67454846?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Meat+intake%2C+cooking-related+mutagens+and+risk+of+colorectal+adenoma+in+a+sigmoidoscopy-based+case-control+study.&rft.au=Gunter%2C+Marc+J%3BProbst-Hensch%2C+Nicole+M%3BCortessis%2C+Victoria+K%3BKulldorff%2C+Martin%3BHaile%2C+Robert+W%3BSinha%2C+Rashmi&rft.aulast=Gunter&rft.aufirst=Marc&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=637&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-13 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Modeling metastasis in vivo. AN - 67454795; 15358632 AB - Metastasis, the spread of a tumor from its primary site to other parts of the body, continues to be the most significant problem in the field of cancer. Patients who present with metastatic disease or those who develop metastases after successful management of the primary tumor carry a universally grave prognosis. To improve treatment outcomes for these patients a broader understanding of the biology of metastases is necessary. The biological complexity that characterizes metastasis requires complex experimental systems for its study. To a large extent the modeling of this biological complexity is only possible using animal models. The following review will summarize the strengths and weaknesses of available in vivo models of metastasis including transplantable syngeneic mouse and human-mouse xenografts, genetically engineered mice and naturally occurring cancers of companion animals (pet dogs and cats). No single metastasis model is sufficient to answer all questions. As such, the selection of the optimal model(s) for each biological or translational question is necessary. JF - Carcinogenesis AU - Khanna, Chand AU - Hunter, Kent AD - Comparative Oncology Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. khannac@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 513 EP - 523 VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - Index Medicus KW - Neoplasm Transplantation KW - Animals KW - Humans KW - Neoplasm Metastasis KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67454795?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Modeling+metastasis+in+vivo.&rft.au=Khanna%2C+Chand%3BHunter%2C+Kent&rft.aulast=Khanna&rft.aufirst=Chand&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=513&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-13 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cutting edge: p27Kip1 deficiency reduces the requirement for CD28-mediated costimulation in naive CD8+ but not CD4+ T lymphocytes. AN - 67454313; 15728451 AB - Cell cycle re-entry of quiescent T cells is dependent upon cyclin-dependent kinase 2. Inhibition of cyclin-dependent kinase 2 by p27(Kip1) is believed to be the principal constraint on S-phase entry in T cells. We report that deficiency for p27(Kip1) has a more pronounced effect on the expansion of murine naive CD8(+) T cells and that this disparity is due to a reduced requirement for CD28-mediated costimulation in CD8(+) but not CD4(+) T cells lacking p27(Kip1). These data highlight a previously unappreciated difference in the way CD28 signaling is coupled to the core cell cycle machinery in these two T cell subsets. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Wolfraim, Lawrence A AU - Letterio, John J AD - Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. wolfraim@tolergenics.com Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 2481 EP - 2484 VL - 174 IS - 5 SN - 0022-1767, 0022-1767 KW - Antigens, CD28 KW - 0 KW - Cdkn1a protein, mouse KW - Cdkn1b protein, mouse KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - Tumor Suppressor Proteins KW - Cyclin-Dependent Kinase Inhibitor p27 KW - 147604-94-2 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Resting Phase, Cell Cycle -- immunology KW - Mice KW - CD4-CD8 Ratio KW - Cell Proliferation KW - Resting Phase, Cell Cycle -- genetics KW - Mice, Knockout KW - Cells, Cultured KW - Kinetics KW - Signal Transduction -- genetics KW - Mice, Inbred C57BL KW - Signal Transduction -- immunology KW - Female KW - Male KW - Tumor Suppressor Proteins -- deficiency KW - CD4-Positive T-Lymphocytes -- cytology KW - CD8-Positive T-Lymphocytes -- metabolism KW - Cell Cycle Proteins -- biosynthesis KW - Antigens, CD28 -- physiology KW - Tumor Suppressor Proteins -- genetics KW - CD4-Positive T-Lymphocytes -- immunology KW - Down-Regulation -- immunology KW - Tumor Suppressor Proteins -- biosynthesis KW - CD8-Positive T-Lymphocytes -- cytology KW - Antigens, CD28 -- genetics KW - Lymphocyte Activation -- genetics KW - CD4-Positive T-Lymphocytes -- metabolism KW - Cell Cycle Proteins -- genetics KW - Down-Regulation -- genetics KW - Lymphocyte Activation -- immunology KW - CD8-Positive T-Lymphocytes -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67454313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Cutting+edge%3A+p27Kip1+deficiency+reduces+the+requirement+for+CD28-mediated+costimulation+in+naive+CD8%2B+but+not+CD4%2B+T+lymphocytes.&rft.au=Wolfraim%2C+Lawrence+A%3BLetterio%2C+John+J&rft.aulast=Wolfraim&rft.aufirst=Lawrence&rft.date=2005-03-01&rft.volume=174&rft.issue=5&rft.spage=2481&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-19 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - 4-Hydroxybutyl(butyl)nitrosamine-induced urinary bladder cancers in mice: characterization of FHIT and survivin expression and chemopreventive effects of indomethacin. AN - 67453690; 15591090 AB - The administration of 4-hydroxybutyl(butyl)nitrosamine (OH-BBN) to male B6D2F1 mice yielded a high incidence of large palpable urinary bladder cancers. Since prior studies demonstrated chemopreventive effects of non-steroidal anti-inflammatory drugs (NSAIDs), we further explored the efficacy of the NSAID indomethacin using different treatment regimens. OH-BBN was administered twice per week for 12 weeks (the first week of treatment was designated week 1). In Experiment I continual indomethacin treatment (20 mg/kg diet) was initiated either prior to (week -1) or following (week 13) OH-BBN dosing. Palpable bladder masses (subsequently diagnosed as cancers) developed in 32% of carcinogen-treated only mice by 32 weeks, while mice administered indomethacin either prior to or after OH-BBN developed palpable masses in 3 and 6% of the animals, respectively. In Experiment II mice were treated with indomethacin beginning 1 week after OH-BBN for either 12 weeks (limited treatment, weeks 13-24) or for 30 weeks (weeks 13-42). Continual treatment resulted in a 77% decrease in palpable bladder masses and an 82% decrease in all cancers (palpable and microscopic), while limited treatment decreased palpable masses by 48% but failed to decrease the number of bladder cancers (palpable plus microscopic). In Experiment III OH-BBN-treated mice were followed for 61 weeks. Palpable masses developed in 66% of control mice, while 26% of mice treated with indomethacin continually from 1 week after OH-BBN (weeks 13-61) developed palpable masses. A separate group in this study treated with indomethacin beginning when 5% of the mice had palpable bladder masses continued to develop new masses for an additional 4 weeks. By 6 weeks after beginning indomethacin treatment, however, these animals showed a profound decrease in the development of additional cancers. The expressions of FHIT and survivin in normal urinary bladder epithelium and in bladder cancers were determined by immunohistochemical analysis. FHIT was expressed at high levels in normal epithelium, but was minimally expressed in cancers, and even showed decreased expression in papillomas. The anti-apoptotic protein survivin was not expressed in normal bladder epithelium, but was variably expressed in cancers. FHIT and survivin expressions were similar in cancers from indomethacin-treated and non-treated mice. JF - Carcinogenesis AU - Lubet, Ronald A AU - Huebner, Kay AU - Fong, Louise Y Y AU - Altieri, Dario C AU - Steele, Vernon E AU - Kopelovich, Levy AU - Kavanaugh, Claudine AU - Juliana, M Margaret AU - Soong, Seng-Jaw AU - Grubbs, Clinton J AD - Division of Cancer Prevention, National Cancer Institute, Executive Plaza North, Suite 2110, 6130 Executive Boulevard, Bethesda, MD 20852, USA. rl57@nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 571 EP - 578 VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - Anti-Inflammatory Agents, Non-Steroidal KW - 0 KW - Birc5 protein, mouse KW - Carcinogens KW - Inhibitor of Apoptosis Proteins KW - Microtubule-Associated Proteins KW - Neoplasm Proteins KW - Repressor Proteins KW - fragile histidine triad protein KW - Butylhydroxybutylnitrosamine KW - 3817-11-6 KW - Acid Anhydride Hydrolases KW - EC 3.6.- KW - Indomethacin KW - XXE1CET956 KW - Index Medicus KW - Animals KW - Mice KW - Immunohistochemistry KW - Male KW - Butylhydroxybutylnitrosamine -- toxicity KW - Microtubule-Associated Proteins -- metabolism KW - Urinary Bladder Neoplasms -- prevention & control KW - Carcinogens -- toxicity KW - Acid Anhydride Hydrolases -- metabolism KW - Neoplasm Proteins -- metabolism KW - Urinary Bladder Neoplasms -- chemically induced KW - Indomethacin -- pharmacology KW - Anti-Inflammatory Agents, Non-Steroidal -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67453690?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=4-Hydroxybutyl%28butyl%29nitrosamine-induced+urinary+bladder+cancers+in+mice%3A+characterization+of+FHIT+and+survivin+expression+and+chemopreventive+effects+of+indomethacin.&rft.au=Lubet%2C+Ronald+A%3BHuebner%2C+Kay%3BFong%2C+Louise+Y+Y%3BAltieri%2C+Dario+C%3BSteele%2C+Vernon+E%3BKopelovich%2C+Levy%3BKavanaugh%2C+Claudine%3BJuliana%2C+M+Margaret%3BSoong%2C+Seng-Jaw%3BGrubbs%2C+Clinton+J&rft.aulast=Lubet&rft.aufirst=Ronald&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-13 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeting malignant B-cell lymphoma with a humanized anti-CD22 scFv-angiogenin immunoenzyme. AN - 67450248; 15725080 AB - We report on the generation and functional characterization of a humanized immunoenzyme comprising a stable humanized single chain Fv (scFv) with grafted specificity of the anti-CD22 murine monoclonal antibody RFB4 and the human ribonuclease angiogenin (ANG). The fusion protein produced from transiently transfected mammalian Chinese hamster ovary cells could easily be purified to homogeneity, retained full ribonucleolytic activity, and efficiently killed CD22(+) tumour cells with an IC(50) of 56 nmol/l. In contrast, incubation of tumour cells with either ANG or scFv alone did not result in any cytotoxicity. Potent receptor-mediated killing of target cells, expected lack of extracellular toxicity, predictable low immunogenic potential, and ease of production, suggest that this novel immunoenzyme has potential for the immunotherapy of CD22(+) malignancies. JF - British journal of haematology AU - Krauss, Jürgen AU - Arndt, Michaela A E AU - Vu, Bang K AU - Newton, Dianne L AU - Rybak, Susanna M AD - SAIC, National Cancer Institute at Frederick, Frederick, MD 21702, USA. juergen.krauss@uni-essen.de Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 602 EP - 609 VL - 128 IS - 5 SN - 0007-1048, 0007-1048 KW - Antigens, CD KW - 0 KW - Antigens, Differentiation, B-Lymphocyte KW - CD22 protein, human KW - Cell Adhesion Molecules KW - Immunoglobulin Fragments KW - Lectins KW - Recombinant Fusion Proteins KW - Sialic Acid Binding Ig-like Lectin 2 KW - angiogenin KW - EC 3.1.27.- KW - Ribonuclease, Pancreatic KW - EC 3.1.27.5 KW - Index Medicus KW - Transfection -- methods KW - Animals KW - Genetic Engineering KW - Humans KW - Cytotoxicity Tests, Immunologic KW - CHO Cells KW - Cell Line, Tumor KW - Recombinant Fusion Proteins -- therapeutic use KW - Cricetinae KW - Lymphoma, B-Cell -- therapy KW - Lymphoma, B-Cell -- immunology KW - Cell Adhesion Molecules -- immunology KW - Antigens, Differentiation, B-Lymphocyte -- immunology KW - Immunization, Passive -- methods KW - Lectins -- immunology KW - Antigens, CD -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67450248?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+journal+of+haematology&rft.atitle=Targeting+malignant+B-cell+lymphoma+with+a+humanized+anti-CD22+scFv-angiogenin+immunoenzyme.&rft.au=Krauss%2C+J%C3%BCrgen%3BArndt%2C+Michaela+A+E%3BVu%2C+Bang+K%3BNewton%2C+Dianne+L%3BRybak%2C+Susanna+M&rft.aulast=Krauss&rft.aufirst=J%C3%BCrgen&rft.date=2005-03-01&rft.volume=128&rft.issue=5&rft.spage=602&rft.isbn=&rft.btitle=&rft.title=British+journal+of+haematology&rft.issn=00071048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-12 N1 - Date created - 2005-02-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - N-substituted piperazines abused by humans mimic the molecular mechanism of 3,4-methylenedioxymethamphetamine (MDMA, or 'Ecstasy'). AN - 67446160; 15496938 AB - 3,4-Methylenedioxymethamphetamine (MDMA, or 'Ecstasy') is an illicit drug that stimulates the release of serotonin (5-HT) and dopamine (DA) from neurons. Recent evidence reveals that drug users are ingesting piperazine analogs, like 1-benzylpiperazine (BZP, or 'A2') and 1-(m-trifluoromethylphenyl)piperazine (TFMPP, or 'Molly'), to mimic psychoactive effects of MDMA. In the present study, we compared the neurochemistry of MDMA, BZP, and TFMPP in rats. The effects of MDMA, BZP, and TFMPP on transporter-mediated efflux of [3H]5-HT and [3H]MPP+ (DA transporter substrate) were determined in synaptosomes. The effects of drugs on extracellular levels of 5-HT and DA were examined using in vivo microdialysis in conscious rats. MDMA evoked transporter-mediated release of [3H]5-HT and [3H]MPP+. BZP released [3H]MPP+, whereas TFMPP was a selective releaser of [3H]5-HT. MDMA (1-3 mg/kg, i.v.) increased dialysate 5-HT and DA in a dose-related fashion, with actions on 5-HT being predominant. BZP (3-10 mg/kg, i.v.) elevated dialysate DA and 5-HT, while TFMPP (3-10 mg/kg, i.v.) elevated 5-HT. Administration of BZP plus TFMPP at a 1:1 ratio (BZP/TFMPP) produced parallel increases in dialysate 5-HT and DA; a 3 mg/kg dose of BZP/TFMPP mirrored the effects of MDMA. At a 10 mg/kg dose, BZP/TFMPP increased dialysate DA more than the summed effects of each drug alone, and some rats developed seizures. Our results show that BZP/TFMPP and MDMA share the ability to evoke monoamine release, but dangerous drug-drug synergism may occur when piperazines are coadministered at high doses. JF - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology AU - Baumann, Michael H AU - Clark, Robert D AU - Budzynski, Allison G AU - Partilla, John S AU - Blough, Bruce E AU - Rothman, Richard B AD - Clinical Psychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. mbaumann@intra.nida.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 550 EP - 560 VL - 30 IS - 3 SN - 0893-133X, 0893-133X KW - Piperazines KW - 0 KW - Receptors, Serotonin KW - Serotonin KW - 333DO1RDJY KW - N-Methyl-3,4-methylenedioxyamphetamine KW - KE1SEN21RM KW - Dopamine KW - VTD58H1Z2X KW - Norepinephrine KW - X4W3ENH1CV KW - Index Medicus KW - Receptors, Serotonin -- physiology KW - Animals KW - Synaptosomes -- drug effects KW - Humans KW - Disease Models, Animal KW - Dopamine -- metabolism KW - Receptors, Serotonin -- metabolism KW - Microdialysis KW - Rats KW - Rats, Sprague-Dawley KW - Kinetics KW - Norepinephrine -- metabolism KW - Serotonin -- metabolism KW - Synaptosomes -- metabolism KW - Male KW - Substance-Related Disorders KW - N-Methyl-3,4-methylenedioxyamphetamine -- pharmacology KW - Piperazines -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67446160?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.atitle=N-substituted+piperazines+abused+by+humans+mimic+the+molecular+mechanism+of+3%2C4-methylenedioxymethamphetamine+%28MDMA%2C+or+%27Ecstasy%27%29.&rft.au=Baumann%2C+Michael+H%3BClark%2C+Robert+D%3BBudzynski%2C+Allison+G%3BPartilla%2C+John+S%3BBlough%2C+Bruce+E%3BRothman%2C+Richard+B&rft.aulast=Baumann&rft.aufirst=Michael&rft.date=2005-03-01&rft.volume=30&rft.issue=3&rft.spage=550&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-21 N1 - Date created - 2005-02-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prophylactic high-dose N(omega)-monomethyl-L-arginine prevents the late cardiac dysfunction associated with lethal tumor necrosis factor-alpha challenge in dogs. AN - 67439480; 15718929 AB - We investigated nitric oxide (NO) as a possible cause of the cardiac dysfunction associated with high, lethal doses of tumor necrosis factor-alpha (TNF-alpha) in dogs. Eighty-seven awake, 2-year-old (10-12 kg), purpose-bred beagles were randomized to receive an infusion of saline or N-monomethyl-L-arginine (L-NMMA), a nonselective NO synthase (NOS) inhibitor, as a 40 mg kg bolus followed by a 40 mg kg(-1) h(-1) infusion for 3 to 6 h 3 h before (prophylactic) or 3 h after (therapeutic) challenge with TNF-alpha (60 microg kg(-1)) or vehicle. Serial radionuclide-heart scans and thermodilution pulmonary artery catheter hemodynamic measurements were performed. The effects of prophylactic L-NMMA on TNF-alpha-induced cardiac dysfunction as measured by decreases in mean left ventricular (LV) ejection fraction and downward and rightward shifts of LV function plots (peak systolic pressure versus end systolic volume index and LV stroke work index versus end diastolic volume index) were significantly different comparing early (3-6 h) and delayed (24 h) time points (P = 0.02). Prophylactic L-NMMA therapy did not appear to fully prevent early (3-6 h) TNF-alpha-induced cardiac dysfunction, but at 24 h, complete protection was seen. Therapeutic L-NMMA did not appear to fully protect the heart from TNF-alpha-induced early or delayed cardiac dysfunction (P = NS). Similarly, L-NMMA given prophylactically, but not therapeutically, blocked TNF-alpha-induced increases in exhaled NO flow rates and plasma nitrite and nitrate concentrations (both P = 0.02). These data suggest that TNF-alpha initiates two phases of cardiac injury: an early (3-6 h) phase that may be partially NO independent and a delayed (24 h) phase that is NO dependent. The delayed, more persistent dysfunction can be completely blocked by high doses of a nonselective NOS inhibitor administered before TNF-alpha. JF - Shock (Augusta, Ga.) AU - Sevransky, Jonathan AU - Vandivier, R William AU - Gerstenberger, Eric AU - Correa, Rosalee AU - Ferantz, Victor AU - Banks, Steven M AU - Danner, Robert L AU - Eichacker, Peter Q AU - Natanson, Charles AD - Critical Care Medicine Department, National Institutes of Health, Bethesda, Maryland, USA. sevransj@jhmi.edu Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 281 EP - 288 VL - 23 IS - 3 SN - 1073-2322, 1073-2322 KW - Enzyme Inhibitors KW - 0 KW - Nitrates KW - Nitrites KW - Tumor Necrosis Factor-alpha KW - omega-N-Methylarginine KW - 27JT06E6GR KW - Nitric Oxide KW - 31C4KY9ESH KW - Nitric Oxide Synthase KW - EC 1.14.13.39 KW - Index Medicus KW - Ventricular Function, Left -- drug effects KW - Nitrites -- blood KW - Animals KW - Heart Rate -- drug effects KW - Nitric Oxide Synthase -- antagonists & inhibitors KW - Dogs KW - Nitric Oxide -- metabolism KW - Enzyme Inhibitors -- pharmacology KW - Vascular Resistance -- drug effects KW - Blood Pressure -- drug effects KW - Stroke Volume -- drug effects KW - Nitrates -- blood KW - omega-N-Methylarginine -- therapeutic use KW - Tumor Necrosis Factor-alpha -- toxicity KW - Heart Diseases -- drug therapy KW - omega-N-Methylarginine -- administration & dosage KW - Heart Diseases -- chemically induced KW - Heart Diseases -- prevention & control KW - Heart Diseases -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67439480?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Shock+%28Augusta%2C+Ga.%29&rft.atitle=Prophylactic+high-dose+N%28omega%29-monomethyl-L-arginine+prevents+the+late+cardiac+dysfunction+associated+with+lethal+tumor+necrosis+factor-alpha+challenge+in+dogs.&rft.au=Sevransky%2C+Jonathan%3BVandivier%2C+R+William%3BGerstenberger%2C+Eric%3BCorrea%2C+Rosalee%3BFerantz%2C+Victor%3BBanks%2C+Steven+M%3BDanner%2C+Robert+L%3BEichacker%2C+Peter+Q%3BNatanson%2C+Charles&rft.aulast=Sevransky&rft.aufirst=Jonathan&rft.date=2005-03-01&rft.volume=23&rft.issue=3&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Shock+%28Augusta%2C+Ga.%29&rft.issn=10732322&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-11 N1 - Date created - 2005-02-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Amalgam exposure and neurological function. AN - 67437866; 15713345 AB - Concerns regarding the safety of silver-mercury amalgam fillings continue to be raised in the absence of any direct evidence of harm. The widespread population exposure to amalgam mandated that a thorough investigation be conducted of its potential effects on the nervous system. The National Institute of Dental and Craniofacial Research and U.S. Air Force investigators collaborated in the ongoing Air Force Health Study (AFHS) of Vietnam era veterans. The primary study question involved adverse health effects associated with exposure to herbicides or dioxin. An assessment of exposure to dental amalgam fillings was added to the 1997-1998 health examination to investigate possible associations between amalgam exposure and neurological abnormalities. Our study population consisted of 1663 dentate AFHS participants, comprised of 986 AFHS controls and 677 Ranch Hand veterans who were exposed to dioxin in Vietnam. Two hundred and fifty-two of the participants had confirmed diabetes mellitus. Study outcomes included clinical neurological signs, vibrotactile thresholds, and summary variables for different levels of peripheral neuropathy. A limitation of our study is that our database did not include more sensitive continuous measures such as nerve conduction studies. No significant associations were found between amalgam exposure and clinical neurological signs of abnormal tremor, coordination, station or gait, strength, sensation, or muscle stretch reflexes or for any level of peripheral neuropathy among our study participants. A statistically significant association was detected between amalgam exposure and the continuous vibrotactile sensation response for the combined non-diabetic participants and separately for non-diabetic AFHS controls. No significant association in this measure was detectable for non-diabetic Ranch Hand veterans or among the combined diabetic participants. The association is a sub-clinical finding that was not associated with symptoms, clinically evident signs of neuropathy, or any functional impairment. Overall, we found no association between amalgam exposure and neurological signs or clinically evident peripheral neuropathy. Our findings do not support the hypothesis that exposure to amalgam produces adverse, clinically evident neurological effects. JF - Neurotoxicology AU - Kingman, Albert AU - Albers, James W AU - Arezzo, Joseph C AU - Garabrant, David H AU - Michalek, Joel E AD - Division of Clinical Research and Health Promotion, National Institute of Dental and Craniofacial Research, National Institutes of Health, 45 Center Drive, Room 4As-25U, Bethesda, MD 20892-6401, USA. Albert.Kingman@nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 241 EP - 255 VL - 26 IS - 2 SN - 0161-813X, 0161-813X KW - Dental Amalgam KW - 8049-85-2 KW - Index Medicus KW - United States KW - Humans KW - Diagnostic Techniques, Neurological KW - Middle Aged KW - Male KW - Multivariate Analysis KW - Veterans KW - Peripheral Nervous System Diseases -- epidemiology KW - Dental Amalgam -- adverse effects KW - Peripheral Nervous System Diseases -- chemically induced UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67437866?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurotoxicology&rft.atitle=Amalgam+exposure+and+neurological+function.&rft.au=Kingman%2C+Albert%3BAlbers%2C+James+W%3BArezzo%2C+Joseph+C%3BGarabrant%2C+David+H%3BMichalek%2C+Joel+E&rft.aulast=Kingman&rft.aufirst=Albert&rft.date=2005-03-01&rft.volume=26&rft.issue=2&rft.spage=241&rft.isbn=&rft.btitle=&rft.title=Neurotoxicology&rft.issn=0161813X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-03 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pathogen inactivation technology: cleansing the blood supply. AN - 67437424; 15715679 AB - The calculated residual infectious risk of HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV) from blood transfusion is extremely low. However, the risk of bacterial contamination remains and a variety of other agents including emerging viruses, protozoa and tick-borne agents threaten blood supplies and undermine public confidence in blood safety. Traditional methods of donor screening and testing have limited ability to further reduce disease transmission and cannot prevent an emerging infectious agent from entering the blood supply. Pathogen inactivation technologies have all but eliminated the infectious risks of plasma-derived protein fractions, but as yet no technique has proved sufficiently safe and effective for traditional blood components. Half-way technologies can reduce the risk of pathogen transmission from fresh frozen plasma and cryoprecipitate. Traditional methods of mechanical removal such as washing and filtration have limited success in reducing the risk of cell-associated agents, but methods aimed at sterilizing blood have either proved toxic to the cells or to the recipients of blood components. Several promising methods that target pathogen nucleic acid have recently entered clinical testing. JF - Journal of internal medicine AU - Klein, H G AD - Department of Medicine and Pathology, The Johns Hopkins School of Medicine, Baltimore, MD, USA. hklein@cc.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 224 EP - 237 VL - 257 IS - 3 SN - 0954-6820, 0954-6820 KW - Index Medicus KW - Acquired Immunodeficiency Syndrome -- prevention & control KW - Hepatitis -- blood KW - Blood Platelets -- microbiology KW - Acquired Immunodeficiency Syndrome -- virology KW - Disinfection -- methods KW - Acquired Immunodeficiency Syndrome -- blood KW - Hepatitis -- virology KW - Humans KW - Acquired Immunodeficiency Syndrome -- transmission KW - Hepatitis -- prevention & control KW - Erythrocytes -- microbiology KW - Graft vs Host Disease -- etiology KW - Communicable Disease Control -- methods KW - Blood Transfusion -- adverse effects KW - Blood-Borne Pathogens UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67437424?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+internal+medicine&rft.atitle=Pathogen+inactivation+technology%3A+cleansing+the+blood+supply.&rft.au=Klein%2C+H+G&rft.aulast=Klein&rft.aufirst=H&rft.date=2005-03-01&rft.volume=257&rft.issue=3&rft.spage=224&rft.isbn=&rft.btitle=&rft.title=Journal+of+internal+medicine&rft.issn=09546820&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-31 N1 - Date created - 2005-02-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cannabinoid CB1 receptor antagonists as promising new medications for drug dependence. AN - 67436556; 15525797 AB - This review examines the development of cannabinoid CB(1) receptor antagonists as a new class of therapeutic agents for drug addiction. Abused drugs [alcohol, opiates, Delta(9)-tetrahydrocannabinol (Delta(9)-THC), and psychostimulants, including nicotine] elicit a variety of chronically relapsing disorders by interacting with endogenous neural pathways in the brain. In particular, they share the common property of activating mesolimbic dopamine brain reward systems, and virtually all abused drugs elevate dopamine levels in the nucleus accumbens. Cannabinoid CB(1) receptors are expressed in this brain reward circuit and modulate the dopamine-releasing effects of Delta(9)-THC and nicotine. Rimonabant (SR141716), a CB(1) receptor antagonist, blocks both the dopamine-releasing and discriminative and rewarding effects of Delta(9)-THC in animals. Blockade of CB(1) receptor activity by genetic invalidation also decreases rewarding effects of opiates and alcohol in animals. Although CB(1) receptor blockade is generally ineffective in reducing the self-administration of cocaine in rodents and primates, it reduces the reinstatement of extinguished cocaine-seeking behavior produced by cocaine-associated conditioned stimuli and cocaine-priming injections. Likewise, CB(1) receptor blockade is effective in reducing nicotine-seeking behavior induced by re-exposure to nicotine-associated stimuli. Some of these findings have been recently validated in humans. In clinical trials, Rimonabant blocks the subjective effects of Delta(9)-THC in humans and prevents relapse to smoking in exsmokers. Findings from both clinical and preclinical studies suggest that ligands blocking CB(1) receptors offer a novel approach for patients suffering from drug dependence that may be efficacious across different classes of abused drugs. JF - The Journal of pharmacology and experimental therapeutics AU - Le Foll, Bernard AU - Goldberg, Steven R AD - Preclinical Pharmacology Section, NIDA, NIH, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. blefoll@intra.nida.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 875 EP - 883 VL - 312 IS - 3 SN - 0022-3565, 0022-3565 KW - Receptor, Cannabinoid, CB1 KW - 0 KW - Dronabinol KW - 7J8897W37S KW - Index Medicus KW - Models, Animal KW - Animals KW - Self Administration KW - Reward KW - Conditioning (Psychology) KW - Humans KW - Dronabinol -- pharmacology KW - Brain -- drug effects KW - Receptor, Cannabinoid, CB1 -- antagonists & inhibitors KW - Substance-Related Disorders -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67436556?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.atitle=Cannabinoid+CB1+receptor+antagonists+as+promising+new+medications+for+drug+dependence.&rft.au=Le+Foll%2C+Bernard%3BGoldberg%2C+Steven+R&rft.aulast=Le+Foll&rft.aufirst=Bernard&rft.date=2005-03-01&rft.volume=312&rft.issue=3&rft.spage=875&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+pharmacology+and+experimental+therapeutics&rft.issn=00223565&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The cyp2e1-humanized transgenic mouse: role of cyp2e1 in acetaminophen hepatotoxicity. AN - 67435102; 15576447 AB - The cytochrome P450 (P450) CYP2E1 enzyme metabolizes and activates a wide array of toxicological substrates, including alcohols, the widely used analgesic acetaminophen, acetone, benzene, halothane, and carcinogens such as azoxymethane and dimethylhydrazine. Most studies on the biochemical and pharmacological actions of CYP2E1 are derived from studies with rodents, rabbits, and cultured hepatocytes; therefore, extrapolation of the results to humans can be difficult. Creating "humanized" mice by introducing the human CYP2E1 gene into Cyp2e1-null mice can circumvent this disadvantage. A transgenic mouse line expressing the human CYP2E1 gene was established. Western blot and high-performance liquid chromatography/mass spectrometry analyses revealed human CYP2E1 protein expression and enzymatic activity in the liver of CYP2E1-humanized mice. Treatment of mice with the CYP2E1 inducer acetone demonstrated that human CYP2E1 was inducible in this transgenic model. The response to the CYP2E1 substrate acetaminophen was explored in the CYP2E1-humanized mice. Hepatotoxicity, resulting from the CYP2E1-mediated activation of acetaminophen, was demonstrated in the livers of CYP2E1-humanized mice by elevated serum alanine aminotransferase levels, increased hepatocyte necrosis, and decreased P450 levels. These data establish that in this humanized mouse model, human CYP2E1 is functional and can metabolize and activate different CYP2E1 substrates such as chlorzoxazone, p-nitrophenol, acetaminophen, and acetone. CYP2E1-humanized mice will be of great value for delineating the role of human CYP2E1 in ethanol-induced oxidative stress and alcoholic liver damage. They will also function as an important in vivo tool for predicting drug metabolism and disposition and drug-drug interactions of chemicals that are substrates for human CYP2E1. JF - Drug metabolism and disposition: the biological fate of chemicals AU - Cheung, Connie AU - Yu, Ai-Ming AU - Ward, Jerrold M AU - Krausz, Kristopher W AU - Akiyama, Taro E AU - Feigenbaum, Lionel AU - Gonzalez, Frank J AD - Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, 9000 Rockville Pike, Bethesda, MD 20892, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 449 EP - 457 VL - 33 IS - 3 SN - 0090-9556, 0090-9556 KW - Analgesics, Non-Narcotic KW - 0 KW - Acetone KW - 1364PS73AF KW - Acetaminophen KW - 362O9ITL9D KW - Cytochrome P-450 CYP2E1 KW - EC 1.14.13.- KW - Index Medicus KW - Animals KW - Necrosis KW - Enzyme Induction -- drug effects KW - Microsomes, Liver -- metabolism KW - Humans KW - Mice KW - Mice, Transgenic KW - Male KW - Models, Animal KW - Hepatocytes -- drug effects KW - Cytochrome P-450 CYP2E1 -- biosynthesis KW - Analgesics, Non-Narcotic -- toxicity KW - Hepatocytes -- pathology KW - Cytochrome P-450 CYP2E1 -- genetics KW - Acetaminophen -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67435102?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.atitle=The+cyp2e1-humanized+transgenic+mouse%3A+role+of+cyp2e1+in+acetaminophen+hepatotoxicity.&rft.au=Cheung%2C+Connie%3BYu%2C+Ai-Ming%3BWard%2C+Jerrold+M%3BKrausz%2C+Kristopher+W%3BAkiyama%2C+Taro+E%3BFeigenbaum%2C+Lionel%3BGonzalez%2C+Frank+J&rft.aulast=Cheung&rft.aufirst=Connie&rft.date=2005-03-01&rft.volume=33&rft.issue=3&rft.spage=449&rft.isbn=&rft.btitle=&rft.title=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-25 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Rituximab plus infusional cyclophosphamide, doxorubicin, and etoposide in HIV-associated non-Hodgkin lymphoma: pooled results from 3 phase 2 trials. AN - 67432467; 15550484 AB - Evidence suggests that infusional therapy is a more effective means for administering cytotoxic therapy than intravenous bolus therapy for lymphoma and offers greater potential for therapeutic synergy with rituximab, which has a long half-life. We pooled the results of 3 prospective phase 2 trials evaluating rituximab in combination with 96-hour infusion of cyclophosphamide (187.5-200 mg/m2 per day), doxorubicin (12.5 mg/m2 per day), and etoposide (60 mg/m2 per day) (R-CDE) plus granulocyte-colony-stimulating factor (G-CSF) in 74 patients with HIV-associated, B-cell non-Hodgkin lymphoma, of whom 56 (76%) patients received concurrent highly active antiretroviral therapy (HAART). The complete remission (CR) rate was 70% (95% confidence interval [CI], 59%-81%), and the estimated 2-year failure-free survival and overall survival rates were 59% (95% CI, 47%-71%) and 64% (95% CI, 52%-76%), respectively. Ten (14%) patients had opportunistic infections during or within 3 months of the end of R-CDE, and 17 (23%) patients developed nonopportunistic infections after that time. Six (8%) patients died because of infection; 2 (3%) of those infections were bacterial sepsis during R-CDE, and 4 (5%) were opportunistic infections that occurred between 2 and 8 months after the completion of R-CDE. R-CDE produced a 70% CR rate and a 59% 2-year failure-free survival rate in patients with HIV-associated lymphoma. Consistent with other reports, adding rituximab to cytotoxic therapy in this population may increase the risk for life-threatening infection. Further studies evaluating rituximab in combination with infusional chemotherapy are warranted, but caution is advised. JF - Blood AU - Spina, Michele AU - Jaeger, Ulrich AU - Sparano, Joseph A AU - Talamini, Renato AU - Simonelli, Cecilia AU - Michieli, Mariagrazia AU - Rossi, Giuseppe AU - Nigra, Ezio AU - Berretta, Massimiliano AU - Cattaneo, Chiara AU - Rieger, Armin C AU - Vaccher, Emanuela AU - Tirelli, Umberto AD - Division of Medical Oncology A, National Cancer Institute, Via Pedemontana Occidentale 12, 33081, Aviano (PN) Italy. Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 1891 EP - 1897 VL - 105 IS - 5 SN - 0006-4971, 0006-4971 KW - Antibodies, Monoclonal KW - 0 KW - Antibodies, Monoclonal, Murine-Derived KW - Granulocyte Colony-Stimulating Factor KW - 143011-72-7 KW - Rituximab KW - 4F4X42SYQ6 KW - Etoposide KW - 6PLQ3CP4P3 KW - Doxorubicin KW - 80168379AG KW - Cyclophosphamide KW - 8N3DW7272P KW - Abridged Index Medicus KW - Index Medicus KW - Cyclophosphamide -- administration & dosage KW - Opportunistic Infections KW - Infusions, Intravenous KW - Humans KW - Aged KW - Doxorubicin -- administration & dosage KW - Infection KW - Lymphoma, B-Cell -- drug therapy KW - Lymphoma, B-Cell -- mortality KW - Etoposide -- administration & dosage KW - Adult KW - Antiretroviral Therapy, Highly Active KW - Middle Aged KW - Granulocyte Colony-Stimulating Factor -- administration & dosage KW - Male KW - Female KW - Survival Analysis KW - Remission Induction KW - Lymphoma, AIDS-Related -- mortality KW - Lymphoma, AIDS-Related -- drug therapy KW - Antineoplastic Combined Chemotherapy Protocols -- administration & dosage KW - Antineoplastic Combined Chemotherapy Protocols -- toxicity KW - Antibodies, Monoclonal -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67432467?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Blood&rft.atitle=Rituximab+plus+infusional+cyclophosphamide%2C+doxorubicin%2C+and+etoposide+in+HIV-associated+non-Hodgkin+lymphoma%3A+pooled+results+from+3+phase+2+trials.&rft.au=Spina%2C+Michele%3BJaeger%2C+Ulrich%3BSparano%2C+Joseph+A%3BTalamini%2C+Renato%3BSimonelli%2C+Cecilia%3BMichieli%2C+Mariagrazia%3BRossi%2C+Giuseppe%3BNigra%2C+Ezio%3BBerretta%2C+Massimiliano%3BCattaneo%2C+Chiara%3BRieger%2C+Armin+C%3BVaccher%2C+Emanuela%3BTirelli%2C+Umberto&rft.aulast=Spina&rft.aufirst=Michele&rft.date=2005-03-01&rft.volume=105&rft.issue=5&rft.spage=1891&rft.isbn=&rft.btitle=&rft.title=Blood&rft.issn=00064971&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-24 N1 - Date created - 2005-02-17 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Blood. 2005 Mar 1;105(5):1842 [15747398] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An overview of lung cancer genomics and proteomics. AN - 67431827; 15713815 AB - Lung cancer is the cause of nearly 170,000 cancer deaths in the United States each year, accounting for nearly 25% of all deaths from cancer. The 5-yr survival rate for lung cancer is < 15% from the time of diagnosis. This is largely due to the late stage of diagnosis and the lack of effective treatments, reflecting the need for a better understanding of the mechanisms that underlie lung carcinogenesis. Unlike the study of a single gene, protein, or pathway, genomic and proteomic technologies enable a systematic overview that provides the potential to improve our understanding of this disease. Ultimately, this could improve the diagnosis, prognosis, and clinical management of patients with lung cancer. Here, we review studies that generated profiles of gene and protein expression in lung cancer specimens and relevant model systems, and make recommendations to facilitate the clinical application of these technologies. JF - American journal of respiratory cell and molecular biology AU - Granville, Courtney A AU - Dennis, Phillip A AD - National Cancer Institute/NIH, Building 8, Room 5101, 8901 Wisconsin Avenue, Bethesda, MD 20889, USA. pdennis@nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 169 EP - 176 VL - 32 IS - 3 SN - 1044-1549, 1044-1549 KW - Proteome KW - 0 KW - Index Medicus KW - Protein Array Analysis KW - Gene Expression Profiling KW - Animals KW - Tumor Cells, Cultured KW - Humans KW - Disease Models, Animal KW - Mice KW - Lung Neoplasms -- genetics KW - Lung Neoplasms -- classification KW - Lung Neoplasms -- metabolism KW - Genomics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67431827?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+respiratory+cell+and+molecular+biology&rft.atitle=An+overview+of+lung+cancer+genomics+and+proteomics.&rft.au=Granville%2C+Courtney+A%3BDennis%2C+Phillip+A&rft.aulast=Granville&rft.aufirst=Courtney&rft.date=2005-03-01&rft.volume=32&rft.issue=3&rft.spage=169&rft.isbn=&rft.btitle=&rft.title=American+journal+of+respiratory+cell+and+molecular+biology&rft.issn=10441549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-29 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A randomized, phase II trial of ketoconazole plus alendronate versus ketoconazole alone in patients with androgen independent prostate cancer and bone metastases. AN - 67430883; 15711271 AB - Alendronate (AL), a potent oral bisphosphonate, blocks the secretion of matrix metalloproteinase-2 and the establishment of bone metastases in animal models. Ketoconazole (KT) has demonstrated activity in androgen independent prostate cancer (AIPC). In this study we determined whether KT plus AL produced acceptable disease responses compared with KT alone. As the experimental design, 72 patients with progressive AIPC metastatic to bone were randomized to receive KT (1,200 mg daily) plus hydrocortisone (H) (30 mg daily) with or without AL (40 mg daily). Prostate specific antigen (PSA) consensus criteria and radiographic scans were used to determine the proportion of patients with a PSA decrease, time to progression and response duration. The pharmacokinetics of KT and AL were characterized and changes in circulating angiogenic factors were assessed. At a median potential followup of 23.9 months the proportion of patients with a greater than 50% decrease in PSA was similar in the KT/H/AL and KT/H, groups (50% and 47%, respectively). The median duration of response was 8.9 and 6.3 months in the KT/H/AL and KT/H groups, respectively (p = 0.125). Median progression-free survival was not significantly prolonged in the KT/H/AL group (4.6 vs 3.8 months, p = 0.27). There was no significant difference in overall survival between the 2 treatment arms but there was a trend toward improved survival in the KT/H arm (p = 0.074). Toxicity in the 2 groups was mild and there were no clear associations between changes in circulating angiogenic factor levels and clinical outcomes in either treatment arm. There were no statistically significant differences in response rate, progression-free survival or overall survival between KT/H alone and KT/H plus AL treatment in patients with AIPC. The addition of AL to KT/H may increase the response duration with an acceptable safety profile compared with treatment with KT/H alone. However, the addition of AL offers no survival benefit in patients with AIPC. JF - The Journal of urology AU - Figg, William D AU - Liu, Yinong AU - Arlen, Philip AU - Gulley, James AU - Steinberg, Seth M AU - Liewehr, David J AU - Cox, Michael C AU - Zhai, Suoping AU - Cremers, Serge AU - Parr, Allyson AU - Yang, Xiaowei AU - Chen, Clara C AU - Jones, Elizabeth AU - Dahut, William L AD - Center for Cancer Research, National Cancer Institute, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA. wdfigg@helix.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 790 EP - 796 VL - 173 IS - 3 SN - 0022-5347, 0022-5347 KW - Ketoconazole KW - R9400W927I KW - Alendronate KW - X1J18R4W8P KW - Abridged Index Medicus KW - Index Medicus KW - Drug Therapy, Combination KW - Humans KW - Aged KW - Middle Aged KW - Male KW - Ketoconazole -- pharmacokinetics KW - Prostatic Neoplasms -- pathology KW - Bone Neoplasms -- blood KW - Ketoconazole -- therapeutic use KW - Prostatic Neoplasms -- blood KW - Bone Neoplasms -- drug therapy KW - Alendronate -- therapeutic use KW - Prostatic Neoplasms -- drug therapy KW - Bone Neoplasms -- secondary KW - Alendronate -- pharmacokinetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67430883?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+urology&rft.atitle=A+randomized%2C+phase+II+trial+of+ketoconazole+plus+alendronate+versus+ketoconazole+alone+in+patients+with+androgen+independent+prostate+cancer+and+bone+metastases.&rft.au=Figg%2C+William+D%3BLiu%2C+Yinong%3BArlen%2C+Philip%3BGulley%2C+James%3BSteinberg%2C+Seth+M%3BLiewehr%2C+David+J%3BCox%2C+Michael+C%3BZhai%2C+Suoping%3BCremers%2C+Serge%3BParr%2C+Allyson%3BYang%2C+Xiaowei%3BChen%2C+Clara+C%3BJones%2C+Elizabeth%3BDahut%2C+William+L&rft.aulast=Figg&rft.aufirst=William&rft.date=2005-03-01&rft.volume=173&rft.issue=3&rft.spage=790&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+urology&rft.issn=00225347&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-29 N1 - Date created - 2005-02-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Coronary heart disease after radiotherapy for peptic ulcer disease. AN - 67430389; 15708264 AB - To evaluate the risk of coronary heart disease (CHD) and cerebrovascular disease after radiotherapy (RT) for peptic ulcer disease. Peptic ulcer disease patients treated with RT (n = 1859) or by other means (n = 1860) at the University of Chicago Medical Center between 1936 and 1965, were followed through 1997. The observed numbers of cause-specific deaths were compared with the expected numbers from the general population rates. During RT, 5% of the heart was in the treatment field and the remainder of the heart mostly received scattered radiation. A volume-weighted cardiac dose was computed to describe the average tissue dose to the entire organ. We used Cox proportional hazards regression analysis to analyze the CHD and cerebrovascular disease risk associated with RT, adjusting for confounding factors. Greater than expected CHD mortality was observed among the irradiated patients. The irradiated patients received volume-weighted cardiac doses ranging from 1.6 to 3.9 Gy and the portion of the heart directly in the field received doses of 7.6-18.4 Gy. The CHD risk increased with the cardiac dose (p trend = 0.01). The cerebrovascular disease risk was not associated with the surrogate carotid dose. The excess CHD risk in patients undergoing RT for peptic ulcer disease decades previously indicates the need for long-term follow-up for cardiovascular disease after chest RT. JF - International journal of radiation oncology, biology, physics AU - Carr, Zhanat A AU - Land, Charles E AU - Kleinerman, Ruth A AU - Weinstock, Robert W AU - Stovall, Marilyn AU - Griem, Melvin L AU - Mabuchi, Kiyohiko AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH/DHHS, 6120 Executive Boulevard, EPS 7038, Rockville, MD 20852, USA. Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 842 EP - 850 VL - 61 IS - 3 SN - 0360-3016, 0360-3016 KW - Index Medicus KW - Regression Analysis KW - Risk Factors KW - Radiotherapy Dosage KW - Humans KW - Confounding Factors (Epidemiology) KW - Cohort Studies KW - Adult KW - Smoking -- adverse effects KW - Middle Aged KW - Male KW - Female KW - Cause of Death KW - Peptic Ulcer -- radiotherapy KW - Coronary Disease -- etiology KW - Coronary Disease -- mortality KW - Heart -- radiation effects KW - Stroke -- etiology KW - Stroke -- mortality KW - Radiation Injuries -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67430389?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.atitle=Coronary+heart+disease+after+radiotherapy+for+peptic+ulcer+disease.&rft.au=Carr%2C+Zhanat+A%3BLand%2C+Charles+E%3BKleinerman%2C+Ruth+A%3BWeinstock%2C+Robert+W%3BStovall%2C+Marilyn%3BGriem%2C+Melvin+L%3BMabuchi%2C+Kiyohiko&rft.aulast=Carr&rft.aufirst=Zhanat&rft.date=2005-03-01&rft.volume=61&rft.issue=3&rft.spage=842&rft.isbn=&rft.btitle=&rft.title=International+journal+of+radiation+oncology%2C+biology%2C+physics&rft.issn=03603016&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-24 N1 - Date created - 2005-02-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Int J Radiat Oncol Biol Phys. 2006 Jul 1;65(3):957; author reply 957 [16751083] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Polychlorinated biphenyls and menstrual cycle characteristics. AN - 67420623; 15703533 AB - Experimental studies in nonhuman primates provide evidence that low-level exposure to persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs) may affect aspects of their menstrual cycle, including cycle length, regularity, and bleeding duration. Few studies have examined these associations in humans. We used data from 2314 pregnant women who participated in the Collaborative Perinatal Project, a cohort study that enrolled pregnant women in the 1960s in 12 centers in the United States. Information about usual (prepregnancy) menstrual cycle length, regularity, bleeding duration, and dysmenorrhea was collected at enrollment, and 11 PCBs and p,p'-DDE (1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) were measured in stored blood samples collected during the third trimester of pregnancy. We used multivariate linear and logistic regression to examine the association between organochlorine levels and menstrual cycles, adjusting for demographic factors, cholesterol, and triglycerides. Total PCBs were positively associated with increasing menstrual cycle length (adjusted difference across 5 categories of PCB exposure = 0.7 days, trend-test P value = 0.02). Irregular cycles were slightly more frequent among those in the 2 highest categories of PCB exposure (odds ratio for highest category = 1.5; 95% confidence interval = 0.70-3.3), and there also was some evidence of an association with DDE. The strengths of these associations increased with various exclusions made to decrease potential misclassification of the outcome and the exposures. There was little evidence for associations between DDE or PCBs and bleeding duration, heavy bleeding, or dysmenorrhea. This study supports experimental studies in monkeys showing an effect of low-dose PCB exposure on menstrual function. JF - Epidemiology (Cambridge, Mass.) AU - Cooper, Glinda S AU - Klebanoff, Mark A AU - Promislow, Joanne AU - Brock, John W AU - Longnecker, Matthew P AD - Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA. cooper1@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 191 EP - 200 VL - 16 IS - 2 SN - 1044-3983, 1044-3983 KW - Environmental Pollutants KW - 0 KW - Insecticides KW - Dichlorodiphenyl Dichloroethylene KW - 4M7FS82U08 KW - Polychlorinated Biphenyls KW - DFC2HB4I0K KW - Index Medicus KW - Epidemiologic Studies KW - Humans KW - Cohort Studies KW - Adult KW - Middle Aged KW - Adolescent KW - Female KW - Pregnancy KW - Multivariate Analysis KW - Insecticides -- poisoning KW - Dichlorodiphenyl Dichloroethylene -- poisoning KW - Environmental Pollutants -- poisoning KW - Environmental Exposure KW - Polychlorinated Biphenyls -- poisoning KW - Menstrual Cycle -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67420623?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Epidemiology+%28Cambridge%2C+Mass.%29&rft.atitle=Polychlorinated+biphenyls+and+menstrual+cycle+characteristics.&rft.au=Cooper%2C+Glinda+S%3BKlebanoff%2C+Mark+A%3BPromislow%2C+Joanne%3BBrock%2C+John+W%3BLongnecker%2C+Matthew+P&rft.aulast=Cooper&rft.aufirst=Glinda&rft.date=2005-03-01&rft.volume=16&rft.issue=2&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Epidemiology+%28Cambridge%2C+Mass.%29&rft.issn=10443983&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-10 N1 - Date created - 2005-02-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mutational analysis of the DEAD-box RNA helicase eIF4AII characterizes its interaction with transformation suppressor Pdcd4 and eIF4GI. AN - 67415976; 15661843 AB - Eukaryotic initiation factor (eIF) 4A unwinds secondary and tertiary structures in the 5'-untranslated region of mRNA, permitting translation initiation. Programmed cell death 4 (Pdcd4) is a novel transformation suppressor and eIF4A-binding partner that inhibits eIF4A helicase activity and translation. To elucidate the regions of eIF4A that are functionally significant in binding to Pdcd4, we generated point mutations of eIF4A. Two-hybrid analysis revealed that five eIF4A mutants completely lost binding to Pdcd4 while four eIF4A mutants retained wild-type levels of binding. The residues that, when mutated, inactivated Pdcd4 binding specified ATP binding, ATP hydrolysis, or RNA binding. With the exception of the Q-motif mutant eIF4AP56L, the eIF4A mutants inactivated for Pdcd4 binding were inactivated for binding to eIF4G (GM, GC, or both) and for enhancing translation. Several eIF4A mutants showing wild-type level binding to Pdcd4 were also inactivated for binding to eIF4G and for enhancing translation. Thus, significant dissociation of eIF4A's Pdcd4- and eIF4G-binding regions appears to occur. Because three of the four eIF4A mutants that retained Pdcd4 binding also suppressed translation activity in a dominant-negative manner, the structure that defines the Pdcd4-binding domain of eIF4A may be necessary but is insufficient for translation. A structural homology model of eIF4A shows regions important for binding to Pdcd4 and/or eIF4G lying on the perimeters of the hinge area of eIF4A. A competition experiment revealed that Pdcd4 competes with C-terminal eIF4G for binding to eIF4A. In summary, the Pdcd4-binding domains on eIF4A impact both binding to eIF4G and translation initiation in cells. JF - RNA (New York, N.Y.) AU - Zakowicz, Halina AU - Yang, Hsin-Sheng AU - Stark, Cristi AU - Wlodawer, Alexander AU - Laronde-Leblanc, Nicole AU - Colburn, Nancy H AD - Laboratory of Cancer Prevention, Bldg. 576, Rm. 101, National Cancer Institute, Frederick, MD 21702, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 261 EP - 274 VL - 11 IS - 3 SN - 1355-8382, 1355-8382 KW - DNA Primers KW - 0 KW - EIF4G1 protein, human KW - Eukaryotic Initiation Factor-4G KW - Peptide Fragments KW - Peptide Initiation Factors KW - Eukaryotic Initiation Factor-4A KW - EC 2.7.7.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Protein Biosynthesis KW - Base Sequence KW - Models, Molecular KW - Point Mutation KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Protein Binding KW - Peptide Fragments -- metabolism KW - Peptide Initiation Factors -- metabolism KW - Eukaryotic Initiation Factor-4A -- metabolism KW - Eukaryotic Initiation Factor-4A -- genetics KW - Eukaryotic Initiation Factor-4A -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67415976?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=RNA+%28New+York%2C+N.Y.%29&rft.atitle=Mutational+analysis+of+the+DEAD-box+RNA+helicase+eIF4AII+characterizes+its+interaction+with+transformation+suppressor+Pdcd4+and+eIF4GI.&rft.au=Zakowicz%2C+Halina%3BYang%2C+Hsin-Sheng%3BStark%2C+Cristi%3BWlodawer%2C+Alexander%3BLaronde-Leblanc%2C+Nicole%3BColburn%2C+Nancy+H&rft.aulast=Zakowicz&rft.aufirst=Halina&rft.date=2005-03-01&rft.volume=11&rft.issue=3&rft.spage=261&rft.isbn=&rft.btitle=&rft.title=RNA+%28New+York%2C+N.Y.%29&rft.issn=13558382&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-21 N1 - Date created - 2005-02-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1999 Apr 30;274(18):12236-44 [10212190] RNA. 1998 Jul;4(7):828-36 [9671055] Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14037-42 [10570194] Mol Cell Biol. 2000 Jan;20(2):468-77 [10611225] Mol Cell Biol. 2000 Aug;20(16):6019-29 [10913184] J Biol Chem. 2000 Aug 11;275(32):24776-80 [10811643] Genome Res. 2000 Aug;10(8):1172-84 [10958635] Trends Biochem Sci. 2000 Sep;25(9):423-6 [10973054] Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13080-5 [11087862] J Biol Chem. 2000 Dec 29;275(52):41369-76 [11022043] Oncogene. 2001 Feb 8;20(6):669-76 [11314000] RNA. 2001 Mar;7(3):382-94 [11333019] J Biol Chem. 2001 Jan 26;276(4):2872-9 [11060291] J Biol Chem. 2001 Aug 3;276(31):29111-5 [11408474] J Biol Chem. 2001 Aug 17;276(33):30914-22 [11418588] Mol Cell. 2001 Aug;8(2):251-62 [11545728] Mol Cell Biol. 2003 Jan;23(1):26-37 [12482958] Mol Cell. 2003 Jan;11(1):127-38 [12535527] Oncogene. 2003 Jun 12;22(24):3712-20 [12802278] Biol Cell. 2003 May-Jun;95(3-4):157-67 [12867080] Nat Rev Mol Cell Biol. 2004 Mar;5(3):232-41 [14991003] Mol Cell Biol. 2004 May;24(9):3894-906 [15082783] Oncogene. 2004 Oct 21;23(49):8135-45 [15361828] J Biol Chem. 1985 Jun 25;260(12):7651-8 [3838990] Nature. 1989 Jan 12;337(6203):121-2 [2563148] Mol Cell Biol. 1990 Mar;10(3):1134-44 [2304461] EMBO J. 1992 Jul;11(7):2643-54 [1378397] Mol Cell Biol. 1993 Nov;13(11):6789-98 [8413273] EMBO J. 1994 Mar 1;13(5):1205-15 [8131750] Eur J Biochem. 1996 Mar 15;236(3):747-71 [8665893] Mol Cell Biol. 1997 Dec;17(12):6940-7 [9372926] Mol Cell Biol. 1997 Dec;17(12):7283-94 [9372960] Electrophoresis. 1997 Dec;18(15):2714-23 [9504803] Am J Physiol Cell Physiol. 2004 Dec;287(6):C1541-6 [15317660] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Synthesis, structure-activity relationship, and p210(bcr-abl) protein tyrosine kinase activity of novel AG 957 analogs. AN - 67413833; 15698792 AB - A series of novel, sterically hindered lipophilic analogs of AG 957 was designed and synthesized as potential protein tyrosine kinase (PTK) inhibitors. The in vitro activity, in vivo anti-leukemia activity, and pharmacology of these PTK inhibitors were studied. Some aspects of the structure-activity relationship associated with the carboxylic acid, phenol ring, and linker modifications are discussed. We have demonstrated that the 1,4-hydroquinone moiety is essential for activity and that sterically hindered esters contribute to enhanced in vivo efficacy. Adaphostin (NSC 680410) has emerged as the improved compound with the maximum in vivo anti-leukemia hollow fiber activity, concordant with the original lead compound AG 957. Currently, adaphostin is undergoing preclinical toxicology studies. JF - Bioorganic & medicinal chemistry AU - Kaur, Gurmeet AU - Narayanan, Ven L AU - Risbood, Prabhakar A AU - Hollingshead, Melinda G AU - Stinson, Sherman F AU - Varma, Ravi K AU - Sausville, Edward A AD - Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD 20892, USA. Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 1749 EP - 1761 VL - 13 IS - 5 SN - 0968-0896, 0968-0896 KW - Enzyme Inhibitors KW - 0 KW - Tyrphostins KW - tyrphostin AG957 KW - Protein-Tyrosine Kinases KW - EC 2.7.10.1 KW - Fusion Proteins, bcr-abl KW - EC 2.7.10.2 KW - Index Medicus KW - Structure-Activity Relationship KW - Tyrphostins -- pharmacokinetics KW - Tyrphostins -- chemistry KW - Enzyme Inhibitors -- chemistry KW - Enzyme Inhibitors -- pharmacology KW - Enzyme Inhibitors -- pharmacokinetics KW - Enzyme Inhibitors -- chemical synthesis KW - Protein-Tyrosine Kinases -- metabolism KW - Tyrphostins -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67413833?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioorganic+%26+medicinal+chemistry&rft.atitle=Synthesis%2C+structure-activity+relationship%2C+and+p210%28bcr-abl%29+protein+tyrosine+kinase+activity+of+novel+AG+957+analogs.&rft.au=Kaur%2C+Gurmeet%3BNarayanan%2C+Ven+L%3BRisbood%2C+Prabhakar+A%3BHollingshead%2C+Melinda+G%3BStinson%2C+Sherman+F%3BVarma%2C+Ravi+K%3BSausville%2C+Edward+A&rft.aulast=Kaur&rft.aufirst=Gurmeet&rft.date=2005-03-01&rft.volume=13&rft.issue=5&rft.spage=1749&rft.isbn=&rft.btitle=&rft.title=Bioorganic+%26+medicinal+chemistry&rft.issn=09680896&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-25 N1 - Date created - 2005-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Continuous dopaminergic stimulation reduces risk of motor complications in parkinsonian primates. AN - 67410613; 15698620 AB - Levodopa or short-acting dopamine (DA) agonist treatment of advanced parkinsonian patients exposes striatal DA receptors to non-physiologic intermittent stimulation that contributes to the development of dyskinesias and other motor complications. To determine whether continuous dopaminergic stimulation can delay or prevent onset of motor complications, four MPTP-lesioned, levodopa-naive cynomolgus monkeys were implanted subcutaneously with apomorphine containing ethylene vinyl acetate rods. Three other MPTP-lesioned monkeys received daily injections of apomorphine. Animals receiving apomorphine rods showed improved motor function ('ON' state) within 1 day of implantation, and remained continually 'ON' for the duration of treatment (up to 6 months) without developing dyskinesias. Injected animals also showed similar improvement in motor function after each apomorphine injection. However, these primates remained 'ON' for only 90 min and within 7-10 days all developed severe dyskinesias. Implanted monkeys evidenced local irritation, which was alleviated by steroid co-therapy. JF - Experimental neurology AU - Bibbiani, Francesco AU - Costantini, Lauren C AU - Patel, Raj AU - Chase, Thomas N AD - Experimental Therapeutic Branch, Building 10, Room 5C103, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892-1406, USA. Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 73 EP - 78 VL - 192 IS - 1 SN - 0014-4886, 0014-4886 KW - Antiparkinson Agents KW - 0 KW - Dopamine Agonists KW - Drug Implants KW - Polyvinyls KW - Steroids KW - ethylenevinylacetate copolymer KW - 24937-78-8 KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - 9P21XSP91P KW - Apomorphine KW - N21FAR7B4S KW - Index Medicus KW - Steroids -- therapeutic use KW - Animals KW - Polyvinyls -- administration & dosage KW - Drug Administration Schedule KW - Macaca fascicularis KW - Dose-Response Relationship, Drug KW - Polyvinyls -- adverse effects KW - Inflammation -- chemically induced KW - Disease Models, Animal KW - Inflammation -- drug therapy KW - Recovery of Function -- drug effects KW - Injections, Subcutaneous KW - Male KW - Movement -- drug effects KW - Apomorphine -- administration & dosage KW - Antiparkinson Agents -- adverse effects KW - Apomorphine -- adverse effects KW - Parkinsonian Disorders -- physiopathology KW - Antiparkinson Agents -- administration & dosage KW - Dyskinesias -- etiology KW - Apomorphine -- blood KW - Dyskinesias -- prevention & control KW - Dopamine Agonists -- adverse effects KW - Dopamine Agonists -- administration & dosage KW - Parkinsonian Disorders -- drug therapy KW - Parkinsonian Disorders -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67410613?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+neurology&rft.atitle=Continuous+dopaminergic+stimulation+reduces+risk+of+motor+complications+in+parkinsonian+primates.&rft.au=Bibbiani%2C+Francesco%3BCostantini%2C+Lauren+C%3BPatel%2C+Raj%3BChase%2C+Thomas+N&rft.aulast=Bibbiani&rft.aufirst=Francesco&rft.date=2005-03-01&rft.volume=192&rft.issue=1&rft.spage=73&rft.isbn=&rft.btitle=&rft.title=Experimental+neurology&rft.issn=00144886&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-14 N1 - Date created - 2005-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Airpuff startle probes: an efficacious and less aversive alternative to white-noise. AN - 67349620; 15620795 AB - Fear-potentiated startle (FPS) is an increasingly popular psychophysiological method for the objective assessment of fear and anxiety. Studies applying this method often elicit the startle reflex with loud white-noise stimuli. Such intense stimuli may, however, alter psychological processes of interest by creating unintended emotional or attentional artifacts. Additionally, loud acoustic probes may be unsuitable for use with infants, children, the elderly, and those with hearing damage. Past studies have noted robust and reliable startle reflexes elicited by low intensity airpuffs. The current study compares the aversiveness of white-noise (102 dB) and airpuff (3 psi) probes and examines the sensitivity of each probe for the assessment of fear-potentiated startle. Results point to less physiological arousal and self-reported reactivity to airpuff versus white-noise probes. Additionally, both probes elicited equal startle magnitudes, response probabilities, and levels of fear-potentiated startle. Such results support the use of low intensity airpuffs as efficacious and relatively non-aversive startle probes. JF - Biological psychology AU - Lissek, Shmuel AU - Baas, Johanna M P AU - Pine, Daniel S AU - Orme, Kaebah AU - Dvir, Sharone AU - Nugent, Monique AU - Rosenberger, Emily AU - Rawson, Elizabeth AU - Grillon, Christian AD - Mood and Anxiety Disorders Program, National Institute of Mental Health (NIH), DHHS, 15K North Drive, Bldg 15k, MSC 2670, Bethesda, MD 20892-2670, USA. lisseks@intra.nimh.nih.gov Y1 - 2005/03// PY - 2005 DA - March 2005 SP - 283 EP - 297 VL - 68 IS - 3 SN - 0301-0511, 0301-0511 KW - Index Medicus KW - Emotions KW - Artifacts KW - Reproducibility of Results KW - Anxiety Disorders KW - Acoustics KW - Humans KW - Adult KW - Air Movements KW - Male KW - Female KW - Fear KW - Reflex, Startle -- physiology KW - Noise -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67349620?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biological+psychology&rft.atitle=Airpuff+startle+probes%3A+an+efficacious+and+less+aversive+alternative+to+white-noise.&rft.au=Lissek%2C+Shmuel%3BBaas%2C+Johanna+M+P%3BPine%2C+Daniel+S%3BOrme%2C+Kaebah%3BDvir%2C+Sharone%3BNugent%2C+Monique%3BRosenberger%2C+Emily%3BRawson%2C+Elizabeth%3BGrillon%2C+Christian&rft.aulast=Lissek&rft.aufirst=Shmuel&rft.date=2005-03-01&rft.volume=68&rft.issue=3&rft.spage=283&rft.isbn=&rft.btitle=&rft.title=Biological+psychology&rft.issn=03010511&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-10 N1 - Date created - 2004-12-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pathways to reading: the role of oral language in the transition to reading AN - 38057073; 2915104 AB - What is the role of oral language in reading competence during the transition to school? Is oral language in preschool best conceptualized as vocabulary knowledge or as more comprehensive language including grammar, vocabulary, and semantics? These questions were examined longitudinally using 1,137 children from the National Institute of Child Health and Human Development Study of Early Child Care and Youth Development. Children were followed from age 3 through 3rd grade, and the results suggest that oral language conceptualized broadly plays both a direct and an indirect role in word recognition during the transition to school and serves as a better foundation for early reading skill than does vocabulary alone. Implications of these findings are discussed in terms of both theoretical models of early reading and practical implications for policy and assessment. Reprinted by permission of the American Psychological Association JF - Developmental psychology Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 428 EP - 442 VL - 41 IS - 2 SN - 0012-1649, 0012-1649 KW - Sociology KW - Pre-school education KW - Reading KW - Vocabulary KW - Language KW - Developmental psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/38057073?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Developmental+psychology&rft.atitle=Pathways+to+reading%3A+the+role+of+oral+language+in+the+transition+to+reading&rft.au=&rft.aulast=&rft.aufirst=&rft.date=2005-03-01&rft.volume=41&rft.issue=2&rft.spage=428&rft.isbn=&rft.btitle=&rft.title=Developmental+psychology&rft.issn=00121649&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 10627 11713; 7226; 13341 7226; 3518 10404; 10002 4049 ER - TY - JOUR T1 - Human brain derived cell culture models of HIV-1 infection AN - 20696161; 10263163 AB - During the clinical course of acquired immune deficiency syndrome, infection of the CNS by human immunodeficiency virus-1 (HIV-1) may ultimately result in the impairment of cognitive, behavioral and motor functions. Viral neuropathogenesis involves inflammatory molecules and neurotoxins produced from infected and immune-activated lymphocytes, microglial cells and astrocytes. Here, we discuss the current understanding of HIV-1 infection of the CNS and various cell culture systems from the developing human brain in order to study the neurobiology of HIV-1 infection, the mechanisms contributing to HIV-1 infection, and disease progression. JF - Neurotoxicity Research AU - Seth, Pankaj AU - Major, Eugene O AD - Molecular and Cellular Neuroscience, National Brain Research Centre, Manesar, India, major@ninds.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 83 EP - 89 PB - Taylor & Francis Group Ltd., 2 Park Square Milton Park, Abingdon Oxford OX14 4RN UK, [URL:http://www.taylorandfrancis.co.uk/] VL - 8 IS - 1-2 SN - 1029-8428, 1029-8428 KW - Toxicology Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Central nervous system KW - Acquired immune deficiency syndrome KW - Astrocytes KW - Neuropathogenesis KW - Immunodeficiency KW - Brain KW - Cell culture KW - Lymphocytes KW - Infection KW - Inflammation KW - Nervous system KW - Cognitive ability KW - Human immunodeficiency virus 1 KW - Neurotoxicity KW - Neurotoxins KW - Microglial cells KW - V 22360:AIDS and HIV KW - N3 11028:Neuropharmacology & toxicology KW - W 30945:Fermentation & Cell Culture KW - X 24300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20696161?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurotoxicity+Research&rft.atitle=Human+brain+derived+cell+culture+models+of+HIV-1+infection&rft.au=Seth%2C+Pankaj%3BMajor%2C+Eugene+O&rft.aulast=Seth&rft.aufirst=Pankaj&rft.date=2005-03-01&rft.volume=8&rft.issue=1-2&rft.spage=83&rft.isbn=&rft.btitle=&rft.title=Neurotoxicity+Research&rft.issn=10298428&rft_id=info:doi/10.1007%2FBF03033821 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-08-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Central nervous system; Acquired immune deficiency syndrome; Astrocytes; Brain; Immunodeficiency; Neuropathogenesis; Cell culture; Lymphocytes; Infection; Inflammation; Nervous system; Cognitive ability; Neurotoxicity; Neurotoxins; Microglial cells; Human immunodeficiency virus 1 DO - http://dx.doi.org/10.1007/BF03033821 ER - TY - JOUR T1 - Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry AN - 20642663; 7760291 AB - The finding of angiogenic and vasculogenic cells in the peripheral circulation may have profound effects on the course of a variety of diseases ranging from cancer to cardiovascular disease. These cells are ascribed to be endothelial in nature and are generally referred to as circulating endothelial cells if mature or as endothelial progenitor cells if immature. Different approaches have been used to detect these cells, including in vitro culture, magnetic bead isolation, and flow cytometry. We review flow cytometric methods for the detection and enumeration of these cells and provide technical suggestions to promote the accurate enumeration of circulating endothelial cells and endothelial progenitor cells. JF - Cytometry Part B AU - Khan, Sameena S AU - Solomon, Michael A AU - McCoy Jr, J Philip AD - Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, mccoyj@nhlbi.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 1 EP - 8 PB - John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/] VL - 64B IS - 1 SN - 1552-4949, 1552-4949 KW - Biotechnology Research Abstracts (through 1992) KW - Endothelial cells KW - Flow cytometry KW - Stem cells KW - Reviews KW - Angiogenesis KW - Cell culture KW - Cardiovascular diseases KW - Cancer KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20642663?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cytometry+Part+B&rft.atitle=Detection+of+circulating+endothelial+cells+and+endothelial+progenitor+cells+by+flow+cytometry&rft.au=Khan%2C+Sameena+S%3BSolomon%2C+Michael+A%3BMcCoy+Jr%2C+J+Philip&rft.aulast=Khan&rft.aufirst=Sameena&rft.date=2005-03-01&rft.volume=64B&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Cytometry+Part+B&rft.issn=15524949&rft_id=info:doi/10.1002%2Fcyto.b.20040 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Flow cytometry; Endothelial cells; Stem cells; Cell culture; Reviews; Angiogenesis; Cancer; Cardiovascular diseases DO - http://dx.doi.org/10.1002/cyto.b.20040 ER - TY - JOUR T1 - Biomarkers of Oxidative Stress Study II: Are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning? AN - 20614675; 7983811 AB - Oxidation products of lipids, proteins, and DNA in the blood, plasma, and urine of rats were measured as part of a comprehensive, multilaboratory validation study searching for noninvasive biomarkers of oxidative stress. This article is the second report of the nationwide Biomarkers of Oxidative Stress Study using acute CCl4 poisoning as a rodent model for oxidative stress. The time-dependent (2, 7, and 16 h) and dose-dependent (120 and 1200 mg/kg ip) effects of CCl4 on concentrations of lipid hydroperoxides, TBARS, malondialdehyde (MDA), isoprostanes, protein carbonyls, methionine sulfoxidation, tyrosine products, 8-hydroxy-2'-deoxyguanosine (8-OHdG), leukocyte DNA-MDA adducts, and DNA-strand breaks were investigated to determine whether the oxidative effects of CCl4 would result in increased generation of these oxidation products. Plasma concentrations of MDA and isoprostanes (both measured by GC-MS) and urinary concentrations of isoprostanes (measured with an immunoassay or LC/MS/MS) were increased in both low-dose and high-dose CCl4-treated rats at more than one time point. The other urinary markers (MDA and 8-OHdG) showed significant elevations with treatment under three of the four conditions tested. It is concluded that measurements of MDA and isoprostanes in plasma and urine as well as 8-OHdG in urine are potential candidates for general biomarkers of oxidative stress. All other products were not changed by CCl4 or showed fewer significant effects. JF - Free Radical Biology and Medicine AU - Kadiiska, M B AU - Gladen, B C AU - Baird, D D AU - Germolec, D AU - Graham, L B AU - Parker, C E AU - Nyska, A AU - Wachsman, J T AU - Ames, B N AU - Basu, S AU - Brot, N AU - Fitzgerald, G A AU - Floyd, R A AU - George, M AU - Heinecke, J W AU - Hatch, G E AU - Hensley, K AU - Lawson, J A AU - Marnett, L J AU - Morrow, J D AU - Murray, D M AU - Plastaras, J AU - Roberts II, L J AU - Rokach, J AU - Shigenaga, M K AU - Sohal, R S AU - Sun, J AU - Tice, R R AU - Van Thiel, D H AU - Wellner, D AU - Walter, P B AU - Tomer, K B AU - Mason, R P AU - Barrett, J C AD - Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, P.O. Box 12233, MD F0-02, Research Triangle Park, NC 27709, USA, Kadiiska@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 698 EP - 710 PB - Elsevier Science, The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 38 IS - 6 SN - 0891-5849, 0891-5849 KW - Biochemistry Abstracts 2: Nucleic Acids; Toxicology Abstracts KW - CCl4 KW - Rat KW - Plasma KW - Urine KW - Lipid hydroperoxides KW - TBARS KW - MDA KW - Isoprostanes KW - Protein carbonyls KW - Methionine sulfoxidation KW - Tyrosine products KW - 8-OHdG KW - M1G KW - DNA strand breaks KW - Free radicals KW - Lipids KW - Adducts KW - Leukocytes KW - Poisoning KW - Tyrosine KW - biomarkers KW - Lipid peroxidation KW - Methionine KW - Blood KW - Oxidative stress KW - sulfoxidation KW - DNA KW - Immunoassays KW - carbonyls KW - Malondialdehyde KW - N 14845:Miscellaneous KW - X 24300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20614675?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+Radical+Biology+and+Medicine&rft.atitle=Biomarkers+of+Oxidative+Stress+Study+II%3A+Are+oxidation+products+of+lipids%2C+proteins%2C+and+DNA+markers+of+CCl4+poisoning%3F&rft.au=Kadiiska%2C+M+B%3BGladen%2C+B+C%3BBaird%2C+D+D%3BGermolec%2C+D%3BGraham%2C+L+B%3BParker%2C+C+E%3BNyska%2C+A%3BWachsman%2C+J+T%3BAmes%2C+B+N%3BBasu%2C+S%3BBrot%2C+N%3BFitzgerald%2C+G+A%3BFloyd%2C+R+A%3BGeorge%2C+M%3BHeinecke%2C+J+W%3BHatch%2C+G+E%3BHensley%2C+K%3BLawson%2C+J+A%3BMarnett%2C+L+J%3BMorrow%2C+J+D%3BMurray%2C+D+M%3BPlastaras%2C+J%3BRoberts+II%2C+L+J%3BRokach%2C+J%3BShigenaga%2C+M+K%3BSohal%2C+R+S%3BSun%2C+J%3BTice%2C+R+R%3BVan+Thiel%2C+D+H%3BWellner%2C+D%3BWalter%2C+P+B%3BTomer%2C+K+B%3BMason%2C+R+P%3BBarrett%2C+J+C&rft.aulast=Kadiiska&rft.aufirst=M&rft.date=2005-03-01&rft.volume=38&rft.issue=6&rft.spage=698&rft.isbn=&rft.btitle=&rft.title=Free+Radical+Biology+and+Medicine&rft.issn=08915849&rft_id=info:doi/10.1016%2Fj.freeradbiomed.2004.09.017 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Adducts; Lipids; Leukocytes; Poisoning; Tyrosine; biomarkers; Methionine; Lipid peroxidation; Blood; Oxidative stress; Isoprostanes; Urine; sulfoxidation; DNA; carbonyls; Immunoassays; Malondialdehyde DO - http://dx.doi.org/10.1016/j.freeradbiomed.2004.09.017 ER - TY - JOUR T1 - Inverse treatment planning based on MRI for HDR prostate brachytherapy AN - 20541571; 8067293 AB - Purpose To develop and optimize a technique for inverse treatment planning based solely on magnetic resonance imaging (MRI) during high-dose-rate brachytherapy for prostate cancer. Methods and materials Phantom studies were performed to verify the spatial integrity of treatment planning based on MRI. Data were evaluated from 10 patients with clinically localized prostate cancer who had undergone two high-dose-rate prostate brachytherapy boosts under MRI guidance before and after pelvic radiotherapy. Treatment planning MRI scans were systematically evaluated to derive a class solution for inverse planning constraints that would reproducibly result in acceptable target and normal tissue dosimetry. Results We verified the spatial integrity of MRI for treatment planning. MRI anatomic evaluation revealed no significant displacement of the prostate in the left lateral decubitus position, a mean distance of 14.47 mm from the prostatic apex to the penile bulb, and clear demarcation of the neurovascular bundles on postcontrast imaging. Derivation of a class solution for inverse planning constraints resulted in a mean target volume receiving 100% of the prescribed dose of 95.69%, while maintaining a rectal volume receiving 75% of the prescribed dose of <5% (mean 1.36%) and urethral volume receiving 125% of the prescribed dose of <2% (mean 0.54%). Conclusion Systematic evaluation of image spatial integrity, delineation uncertainty, and inverse planning constraints in our procedure reduced uncertainty in planning and treatment. JF - International Journal of Radiation Oncology, Biology, & Physics AU - Citrin, Deborah AU - Ning, Holly AU - Guion, Peter AU - Li, Guang AU - Susil, Robert C AU - Miller, Robert W AU - Lessard, Etienne AU - Pouliot, Jean AU - Huchen, Xie AU - Capala, Jacek AU - Coleman, C Norman AU - Camphausen, Kevin AU - Menard, Cynthia AD - Radiation Oncology Branch, CCR, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, citrind@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 1267 EP - 1275 PB - Elsevier Science, Box 882 New York NY 10159 USA, [mailto:usinfo-f@elsevier.com] VL - 61 IS - 4 SN - 0360-3016, 0360-3016 KW - Biotechnology and Bioengineering Abstracts KW - Brachytherapy KW - High dose rate KW - Inverse planning KW - MRI KW - Prostate KW - Pelvis KW - Prostate cancer KW - Rectum KW - Magnetic resonance imaging KW - Dosimetry KW - Radiotherapy KW - Penis KW - Bulbs KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20541571?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Radiation+Oncology%2C+Biology%2C+%26+Physics&rft.atitle=Inverse+treatment+planning+based+on+MRI+for+HDR+prostate+brachytherapy&rft.au=Citrin%2C+Deborah%3BNing%2C+Holly%3BGuion%2C+Peter%3BLi%2C+Guang%3BSusil%2C+Robert+C%3BMiller%2C+Robert+W%3BLessard%2C+Etienne%3BPouliot%2C+Jean%3BHuchen%2C+Xie%3BCapala%2C+Jacek%3BColeman%2C+C+Norman%3BCamphausen%2C+Kevin%3BMenard%2C+Cynthia&rft.aulast=Citrin&rft.aufirst=Deborah&rft.date=2005-03-01&rft.volume=61&rft.issue=4&rft.spage=1267&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Radiation+Oncology%2C+Biology%2C+%26+Physics&rft.issn=03603016&rft_id=info:doi/10.1016%2Fj.ijrobp.2004.11.024 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-04-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Pelvis; Rectum; Prostate cancer; Brachytherapy; Dosimetry; Magnetic resonance imaging; Radiotherapy; Penis; Bulbs DO - http://dx.doi.org/10.1016/j.ijrobp.2004.11.024 ER - TY - JOUR T1 - Diagnostic and Prognostic Value of Early MR Imaging Vessel Signs in Hyperacute Stroke Patients Imaged <3 Hours and Treated with Recombinant Tissue Plasminogen Activator AN - 20235142; 6187178 AB - BACKGROUND: AND PURPOSE: Analogous to the CT hyperattenuated vessel sign (HMCAS), MR imaging may show hypo-or hyperintense vessels in acute ischemic stroke (AIS) patients. We assessed the diagnostic and prognostic strength of early MR imaging vessel signs in AIS patients treated with intravenous thrombolysis (IVT) within 3 hours of the onset of symptoms. METHODS: We studied AIS patients both treated with IVT and stroke MR imaged within 3 hours of the onset of symptoms and at 2 hours and 24 hours after treatment. We assessed the presence or absence of early vessel signs (hyperintense fluid-attenuated inversion recovery sign [FLAIR HVS]; gradient-echo susceptibility vessel sign [GRE SVS]) compared with a combined MR angiography/perfusion-weighted imaging reference and their strength for predicting clinical outcome (favorable vs. poor, independent vs. dependent, or dead, death), recanalization (by clot composition and flow), and hemorrhage in uni-and multivariate analysis. RESULTS: Fifty-six patients (age range, 76 years +/-13 years; median National Institutes of Health stroke scale score [NIHSSS], 11) met the inclusion criteria. Forty-four patients (78.6%) had a vessel occlusion at baseline; 22 of them (50%) recanalized. Nineteen patients (33.9%) suffered some form of intracranial hemorrhage (ICH), 24 patients (42.9%) had an independent outcome, 18 patients (32.1%) a favorable outcome, and 14 patients died. Compared with our combined reference for vessel status PWI/MRA, the sensitivities of CT HMCAS, FLAIR HVS, and GRE SVS were 40%, 66%, and 34%, respectively, and improved during the hours that followed. Localization was accurately reflected by FLAIR HVS but not by GRE SVS. Only NIHSSS and age were independent predictors for recanalization and all clinical outcomes in multiple logistic regression analysis. CONCLUSION: Although early vessel signs can be helpful in the diagnosis of intravascular disease, they do not independently predict recanalization, ICH, or any of the three clinical outcomes in a multivariate logistic regression model. Thrombus composition as reflected by signal intensity characteristics on GRE and FLAIR does not predict the therapeutic effect of IVT. JF - American Journal of Neuroradiology AU - Schellinger, Peter D AU - Chalela, Julio A AU - Kang, Dong-Wha AU - Latour, Lawrence L AU - Warach, Steven AD - National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. Department of Neurology, University of Heidelberg, Heidelberg, Germany Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 618 EP - 624 PB - American Society of Neuroradiology, 2210 Midwest Rd. Ste. 207 Oak Brood IL 60521 USA, [mailto:ajnr@interaccess.com] VL - 26 IS - 3 SN - 0195-6108, 0195-6108 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - thrombolysis KW - Intravenous administration KW - Age KW - Magnetic resonance imaging KW - Stroke KW - Ischemia KW - Hemorrhage KW - t-Plasminogen activator KW - Models KW - Angiography KW - Inversion KW - Multivariate analysis KW - Occlusion KW - Computed tomography KW - Regression analysis KW - W 30910:Imaging KW - N3 11027:Neurology & neuropathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20235142?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Neuroradiology&rft.atitle=Diagnostic+and+Prognostic+Value+of+Early+MR+Imaging+Vessel+Signs+in+Hyperacute+Stroke+Patients+Imaged+%26lt%3B3+Hours+and+Treated+with+Recombinant+Tissue+Plasminogen+Activator&rft.au=Schellinger%2C+Peter+D%3BChalela%2C+Julio+A%3BKang%2C+Dong-Wha%3BLatour%2C+Lawrence+L%3BWarach%2C+Steven&rft.aulast=Schellinger&rft.aufirst=Peter&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=618&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Neuroradiology&rft.issn=01956108&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Age; Intravenous administration; thrombolysis; Stroke; Magnetic resonance imaging; Ischemia; Hemorrhage; t-Plasminogen activator; Models; Angiography; Multivariate analysis; Inversion; Occlusion; Computed tomography; Regression analysis ER - TY - JOUR T1 - Clinical Issues and Research in Respiratory Failure from Severe Acute Respiratory Syndrome AN - 199626630; 15591472 AB - The National Heart, Lung, and Blood Institute, along with the Centers for Disease Control and Prevention and the National Institute of Allergy and Infectious Diseases, convened a panel to develop recommendations for treatment, prevention, and research for respiratory failure from severe acute respiratory syndrome (SARS) and other newly emerging infections. The clinical and pathological features of acute lung injury (ALI) from SARS appear indistinguishable from ALI from other causes. The mainstay of treatments for ALI remains supportive. Patients with ALI from SARS who require mechanical ventilation should receive a lung protective, low tidal volume strategy. Adjuvant treatments recommended include prevention of venous thromboembolism, stress ulcer prophylaxis, and semirecumbent positioning during ventilation. Based on previous experience in Canada, infection control resources and protocols were recommended. Leadership structure, communication, training, and morale are an essential aspect of SARS management. A multicenter, placebo-controlled trial of corticosteroids for late SARS is justified because of widespread clinical use and uncertainties about relative risks and benefits. Studies of combined pathophysiologic endpoints were recommended, with mortality as a secondary endpoint. The group recommended preparation for studies, including protocols, ethical considerations, Web-based registries, and data entry systems. JF - American Journal of Respiratory and Critical Care Medicine AU - Levy, Mitchell M AU - Baylor, Melisse S AU - Bernard, Gordon R AU - Fowler, Rob AU - et al Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 518 EP - 26 CY - New York PB - American Thoracic Society VL - 171 IS - 5 SN - 1073449X KW - Medical Sciences--Respiratory Diseases KW - Adrenal Cortex Hormones KW - Antiviral Agents KW - United States KW - Antiviral Agents -- therapeutic use KW - Pulmonary Medicine -- methods KW - Pneumonia -- prevention & control KW - Pulmonary Medicine -- trends KW - Humans KW - Respiratory Insufficiency -- etiology KW - Infection Control -- organization & administration KW - Lung -- pathology KW - Research -- trends KW - Community-Acquired Infections -- prevention & control KW - Adrenal Cortex Hormones -- therapeutic use KW - Severe Acute Respiratory Syndrome -- complications KW - Canada KW - Infection Control -- methods KW - Respiration, Artificial -- methods KW - Severe Acute Respiratory Syndrome -- pathology KW - Community-Acquired Infections -- complications KW - Pneumonia -- complications KW - Clinical Protocols -- standards KW - Pulmonary Medicine -- standards KW - Infection Control -- standards KW - Severe Acute Respiratory Syndrome -- therapy KW - Respiratory Insufficiency -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/199626630?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=unknown&rft.jtitle=American+Journal+of+Respiratory+and+Critical+Care+Medicine&rft.atitle=Clinical+Issues+and+Research+in+Respiratory+Failure+from+Severe+Acute+Respiratory+Syndrome&rft.au=Levy%2C+Mitchell+M%3BBaylor%2C+Melisse+S%3BBernard%2C+Gordon+R%3BFowler%2C+Rob%3Bet+al&rft.aulast=Levy&rft.aufirst=Mitchell&rft.date=2005-03-01&rft.volume=171&rft.issue=5&rft.spage=518&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Respiratory+and+Critical+Care+Medicine&rft.issn=1073449X&rft_id=info:doi/ LA - English DB - ProQuest Central N1 - Copyright - Copyright American Thoracic Society Mar 1, 2005 N1 - Last updated - 2017-01-07 ER - TY - JOUR T1 - Annotation: PANDAS: a model for human autoimmune disease AN - 19836354; 6800595 AB - Background:Pediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcus infections (PANDAS) is a recently recognized syndrome in which pre-adolescent children have abrupt onsets of tics and/or obsessive-compulsive symptoms, a recurring and remitting course of illness temporally related to streptococcal infections, and associated neurologic findings including adventitious movements, hyperactivity and emotional lability. Methods:Inspired by observations of similar symptoms in children with Sydenham's chorea, a search was undertaken for clinical and laboratory evidence in support of the new syndrome. Results:Consistent and predictable clinical findings have been described in a large case series. Magnetic resonance imaging has supported the postulated pathobiology of the syndrome with evidence of inflammatory changes in basal ganglia. Antibasal ganglia antibodies have been found in some acute cases, mimicking streptococcal antigen epitopes. Conclusions:While PANDAS remains a controversial diagnostic concept, it has stimulated new research endeavors into the possible links between bacterial pathogens, autoimmune reactions, and neuropsychiatric symptoms. JF - Journal of Child Psychology and Psychiatry and Allied Disciplines AU - Swedo, Susan E AU - Grant, Paul J AD - National Institute of Mental Health, Bethesda, Maryland, USA, paul.grant@nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 227 EP - 234 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 46 IS - 3 SN - 0021-9630, 0021-9630 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts; CSA Neurosciences Abstracts KW - Streptococcus KW - Mimicry KW - Emotions KW - Autoimmune diseases KW - Magnetic resonance imaging KW - Pathogens KW - Children KW - Infection KW - Inflammation KW - Chorea KW - Antibodies KW - Mental disorders KW - Obsessive compulsive disorder KW - Lability KW - Basal ganglia KW - Epitopes KW - Hyperactivity KW - N3 11001:Behavioral and Cognitive Neuroscience KW - J 02400:Human Diseases KW - F 06930:Autoimmunity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19836354?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Child+Psychology+and+Psychiatry+and+Allied+Disciplines&rft.atitle=Annotation%3A+PANDAS%3A+a+model+for+human+autoimmune+disease&rft.au=Swedo%2C+Susan+E%3BGrant%2C+Paul+J&rft.aulast=Swedo&rft.aufirst=Susan&rft.date=2005-03-01&rft.volume=46&rft.issue=3&rft.spage=227&rft.isbn=&rft.btitle=&rft.title=Journal+of+Child+Psychology+and+Psychiatry+and+Allied+Disciplines&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2004.00386.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - SuppNotes - Tables, 1. N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Emotions; Mimicry; Magnetic resonance imaging; Autoimmune diseases; Pathogens; Infection; Children; Inflammation; Chorea; Mental disorders; Antibodies; Obsessive compulsive disorder; Lability; Epitopes; Basal ganglia; Hyperactivity; Streptococcus DO - http://dx.doi.org/10.1111/j.1469-7610.2004.00386.x ER - TY - JOUR T1 - Measurement of eight scalar and dipolar couplings for methine-methylene pairs in proteins and nucleic acids AN - 19828028; 6533552 AB - A new 3D, spin-state-selective coherence transfer NMR experiment is described that yields accurate measurements for eight scalar or dipolar couplings within a spin system composed of a methylene adjacent to a methine group. Implementations of the experiment have been optimized for proteins and for nucleic acids. The experiments are demonstrated for C super( beta )-C super( alpha ) moieties of the third IgG-binding domain from Streptococcal Protein G (GB3) and for C[Equation]-C[Equation] groups in a 24-nt RNA oligomer. Chemical shifts of C super( alpha ), C super( beta ) and H super( beta ) (respectively C[Equation], C[Equation] and H[Equation]) are dispersed in the three orthogonal dimensions, and the absence of heteronuclear decoupling leads to distinct and well-resolved E.COSY multiplet patterns. In an isotropic sample, the E.COSY displacements correspond to super(1)J sub(C alpha H alpha ), super(2)J sub(C alpha H beta 2)+ super(2)J sub(C alpha H beta 3), super(2)J sub(C beta H alpha ), super(1)J sub(C beta H beta 2)+ super(1)J sub(C beta H beta 3), super(1)J sub(C beta H beta 2)- super(2)J sub(H beta 2H beta 3), super(1)J sub(C beta H beta 3)- super(2)J sub(H beta 2H beta 3), super(3)J sub(H alpha H beta 2) and super(3)J sub(H alpha H beta 3) for proteins, and super(1)J[Equation], super(2)J[Equation]J[Equation], super(2)J[Equation], super(1)J[Equation]+ super(1)J[Equation], super(1)J[Equation]J[Equation], super(1)J[Equation]J[Equation], super(3)J[Equation] and super(3)J[Equation] in nucleic acids. The experiment, based on relaxation-optimized spectroscopy, yields best results when applied to residues where the methine-methylene group corresponds to a reasonably isolated spin system, as applies for C, F, Y, W, D, N and H residues in proteins, or the C[Equation]-C[Equation] groups in nucleic acids. Splittings can be measured under either isotropic or weakly aligned conditions, yielding valuable structural information both through the super(3)J couplings and the one-, two- and three-bond dipolar interactions. Dipolar couplings for 10 out of 13 sidechains in GB3 are found to be in excellent agreement with its X-ray structure, whereas one residue adopts a different backbone geometry, and two residues are subject to extensive chi sub(1) rotamer averaging. The abundance of dipolar couplings can also yield stereospecific assignments of the non-equivalent methylene protons. For the RNA oligomer, dipolar data yielded stereospecific assignments for six out of the eight C[Equation]H sub(2) groups in the loop region of the oligomer, in all cases confirmed by super(1)J[Equation]J[Equation], and H[Equation] resonating downfield of H[Equation]. JF - Journal of Biomolecular NMR AU - Miclet, Emeric AU - Boisbouvier, Jerome AU - Bax, Ad AD - National Institutes of Health, Bethesda, Maryland, 20892-0520, USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 201 EP - 216 PB - Springer-Verlag (Heidelberg), Tiergartenstrasse 17 Heidelberg 69121 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 31 IS - 3 SN - 0925-2738, 0925-2738 KW - Microbiology Abstracts B: Bacteriology; Biotechnology and Bioengineering Abstracts KW - Streptococcus KW - nucleic acids KW - Data processing KW - RNA KW - Protons KW - streptococcal protein G KW - Ionizing radiation KW - Abundance KW - N.M.R. KW - Spectroscopy KW - Splitting KW - W 30910:Imaging KW - J 02330:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19828028?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biomolecular+NMR&rft.atitle=Measurement+of+eight+scalar+and+dipolar+couplings+for+methine-methylene+pairs+in+proteins+and+nucleic+acids&rft.au=Miclet%2C+Emeric%3BBoisbouvier%2C+Jerome%3BBax%2C+Ad&rft.aulast=Miclet&rft.aufirst=Emeric&rft.date=2005-03-01&rft.volume=31&rft.issue=3&rft.spage=201&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biomolecular+NMR&rft.issn=09252738&rft_id=info:doi/10.1007%2Fs10858-005-0175-z LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Data processing; nucleic acids; RNA; Protons; Ionizing radiation; streptococcal protein G; Abundance; N.M.R.; Spectroscopy; Splitting; Streptococcus DO - http://dx.doi.org/10.1007/s10858-005-0175-z ER - TY - JOUR T1 - Measurement of the Osmotic Properties of Thin Polymer Films and Biological Tissue Samples AN - 19425862; 6655040 AB - A new type of micro-osmometer is described in which water absorption of small tissue samples is measured by a quartz crystal microbalance (QCM). The swelling of the sample deposited on the surface of a quartz crystal is determined by monitoring the change in resonance frequency of the quartz sensor as a function of the vapor pressure in the surrounding environment. The measurement principle is verified by studying the water uptake of poly(vinyl alcohol) films. Reasonable agreement is found between the results obtained by the QCM-based osmometer and previous osmotic pressure measurements made on a similar poly(vinyl alcohol) sample. The feasibility of the new method is demonstrated by measuring the osmotic response of tissue-engineered cartilage samples. It is found that the osmotic pressure of cartilage substantially increases with culture time. The present result is consistent with cartilage models, suggesting that the proteoglycan content governs the compressive resistance of the tissue. JF - Biomacromolecules AU - Horkay, F AU - Horkayne-Szakaly, I AU - Basser, P J AD - Section on Tissue Biophysics and Biomimetics, Laboratory of Integrative and Medical Biophysics, NICHD, National Institutes of Health, 13 South Drive, Bethesda, Maryland 20892, USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 988 EP - 993 VL - 6 IS - 2 SN - 1525-7797, 1525-7797 KW - Biotechnology and Bioengineering Abstracts KW - Cartilage KW - Crystals KW - Tissue engineering KW - Osmotic pressure KW - Models KW - Water uptake KW - Proteoglycans KW - Vapors KW - Quartz KW - alcohols KW - Pressure KW - Films KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19425862?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomacromolecules&rft.atitle=Measurement+of+the+Osmotic+Properties+of+Thin+Polymer+Films+and+Biological+Tissue+Samples&rft.au=Horkay%2C+F%3BHorkayne-Szakaly%2C+I%3BBasser%2C+P+J&rft.aulast=Horkay&rft.aufirst=F&rft.date=2005-03-01&rft.volume=6&rft.issue=2&rft.spage=988&rft.isbn=&rft.btitle=&rft.title=Biomacromolecules&rft.issn=15257797&rft_id=info:doi/10.1021%2Fbm049332c LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Quartz; Cartilage; Films; alcohols; Crystals; Osmotic pressure; Proteoglycans; Water uptake; Vapors; Models; Tissue engineering; Pressure DO - http://dx.doi.org/10.1021/bm049332c ER - TY - JOUR T1 - Accurate measurement of super(15)N- super(13)C residual dipolar couplings in nucleic acids AN - 19420834; 6533554 AB - New 3D HCN quantitative J (QJ) pulse schemes are presented for the precise and accurate measurement of one-bond super(15)N sub(1/9)- super(13)C sub(1'), super(15)N sub(1/9)- super(13)C sub(6/8), and super(15)N sub(1/9)- super(13)C sub(2/4) residual dipolar couplings (RDCs) in weakly aligned nucleic acids. The methods employ super(1)H- super(13)C multiple quantum (MQ) coherence or TROSY-type pulse sequences for optimal resolution and sensitivity. RDCs are obtained from the intensity ratio of H sub(1')-C sub(1')-N sub(1/9) (MQ-HCN-QJ) or H sub(6/8)-C sub(6/8)-N sub(1/9) (TROSY-HCN-QJ) correlations in two interleaved 3D NMR spectra, with dephasing intervals of zero (reference spectrum) and similar to 1/(J2dNC (attenuated spectrum). The different types of super(15)N- super(13)C couplings can be obtained by using either the 3D MQ-HCN-QJ or TROSY-HCN-QJ pulse scheme, with the appropriate setting of the duration of the constant-time super(15)N evolution period and the offset of two frequency-selective super(13)C pulses. The methods are demonstrated for a uniformly super(13)C, super(15)N-enriched 24-nucleotide stem-loop RNA sequence, helix-35 psi , aligned in the magnetic field using phage Pf1. For measurements of RDCs systematic errors are found to be negligible, and experiments performed on a 1.5 mM helix-35 psi sample result in an estimated precision of ca. 0.07 Hz for super(1)D sub(NC), indicating the utility of the measured RDCs in structure validation and refinement. Indeed, for a complete set of super(15)N sub(1/9)- super(13)C sub(1'), super(15)N sub(1/9)- super(13)C sub(6/8), and super(15)N sub(1/9)- super(13)C sub(2/4) dipolar couplings obtained for the stem nucleotides, the measured RDCs are in excellent agreement with those predicted for an NMR structure of helix-35 psi , refined using independently measured observables, including super(13)C- super(1)H, super(13)C- super(13)C and super(1)H- super(1)H dipolar couplings. JF - Journal of Biomolecular NMR AU - Jaroniec, Christopher P AU - Boisbouvier, Jerome AU - Tworowska, Izabela AU - Nikonowicz, Edward P AU - Bax, Ad AD - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 231 EP - 241 PB - Springer-Verlag (Heidelberg), Tiergartenstrasse 17 Heidelberg 69121 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 31 IS - 3 SN - 0925-2738, 0925-2738 KW - Biotechnology and Bioengineering Abstracts KW - Phages KW - Magnetic fields KW - nucleic acids KW - RNA KW - Nucleotide sequence KW - Phage Pf1 KW - N.M.R. KW - Evolution KW - Nucleotides KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19420834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biomolecular+NMR&rft.atitle=Accurate+measurement+of+super%2815%29N-+super%2813%29C+residual+dipolar+couplings+in+nucleic+acids&rft.au=Jaroniec%2C+Christopher+P%3BBoisbouvier%2C+Jerome%3BTworowska%2C+Izabela%3BNikonowicz%2C+Edward+P%3BBax%2C+Ad&rft.aulast=Jaroniec&rft.aufirst=Christopher&rft.date=2005-03-01&rft.volume=31&rft.issue=3&rft.spage=231&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biomolecular+NMR&rft.issn=09252738&rft_id=info:doi/10.1007%2Fs10858-005-0646-2 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Phage Pf1; N.M.R.; nucleic acids; Magnetic fields; RNA; Nucleotides; Phages; Evolution; Nucleotide sequence DO - http://dx.doi.org/10.1007/s10858-005-0646-2 ER - TY - JOUR T1 - Neurodevelopmental and Growth Outcomes of Extremely Low Birth Weight Infants After Necrotizing Enterocolitis AN - 19412312; 6186004 AB - OBJECTIVES: Necrotizing enterocolitis (NEC) is a significant complication for the premature infant. However, subsequent neurodevelopmental and growth outcomes of extremely low birth weight (ELBW) infants with NEC have not been well described. We hypothesized that ELBW infants with surgically managed (SurgNEC) are at greater risk for poor neurodevelopmental and growth outcomes than infants with medically managed NEC (MedNEC) compared with infants without a history of NEC (NoNEC). The objective of this study was to compare growth, neurologic, and cognitive outcomes among ELBW survivors of SurgNEC and MedNEC with NoNEC at 18 to 22 months' corrected age. METHODS: Multicenter, retrospective analysis was conducted of infants who were born between January 1, 1995, and December 31, 1998, and had a birth weight <1000 g in the National Institute of Child Health and Human Development Neonatal Research Network Registry. Neurodevelopment and growth were assessed at 18 to 22 months' postmenstrual age. [Chi] super(2), t test, and logistic regression analyses were used. RESULTS: A total of 2948 infants were evaluated at 18 to 22 months, 124 of whom were SurgNEC and 121 of whom were MedNEC. Compared with NoNEC, both SurgNEC and MedNEC infants were of lower birth weight and had a greater incidence of late sepsis; SurgNEC but not MedNEC infants were more likely to have received a diagnosis of cystic periventricular leukomalacia and bronchopulmonary dysplasia and been treated with postnatal steroids. Weight, length, and head circumference <10 percentile at 18 to 22 months were significantly more likely among SurgNEC but not MedNEC compared with NoNEC infants. After correction for anthropometric measures at birth and adjusted age at follow-up, all growth parameters at 18 to 22 months for SurgNEC but not MedNEC infants were significantly less than for NoNEC infants. SurgNEC but not MedNEC was a significant independent risk factor for Mental Developmental Index <70 (odds ratio [OR]: 1.61; 95% confidence interval [CI]: 1.05-2.50), Psychomotor Developmental Index <70 (OR: 1.95; 95% CI: 1.25-3.04), and neurodevelopmental impairment (OR: 1.78; 95% CI: 1.17-2.73) compared with NoNEC. CONCLUSIONS: Among ELBW infants, SurgNEC is associated with significant growth delay and adverse neurodevelopmental outcomes at 18 to 22 months' corrected age compared with NoNEC. MedNEC does not seem to confer additional risk. SurgNEC is likely to be associated with greater severity of disease. JF - Pediatrics AU - Hintz, Susan R AU - Kendrick, Douglas E AU - Stoll, Barbara J AU - Vohr, Betty R AU - Fanaroff, Avroy A AU - Donovan, Edward F AU - Poole, WKenneth AU - Blakely, Martin L AU - Wright, Linda AU - Higgins, Rosemary AD - Division of Neonatal and Developmental Medicine, Stanford University School of Medicine, Palo Alto, California. Research Triangle Institute, Research Triangle Park, North Carolina. Department of Pediatrics, Emory University, Atlanta, Georgia. Department of Pediatrics, Women and Infants Hospital, Providence, RI. Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio. Department of Pediatrics, University of Cincinnati, Cincinnati, Ohio. Department of Pediatric Surgery, University of Texas, Houston, TX. National Institute of Child Health and Human Development, Washington, DC Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 696 EP - 703 PB - American Academy of Pediatrics, 141 Northwest Point Blvd. Elk Grove Village IL 60007-1098 USA, [mailto:journals@aap.org], [URL:http://www.aap.org] VL - 115 IS - 3 SN - 0031-4005, 0031-4005 KW - Microbiology Abstracts B: Bacteriology; CSA Neurosciences Abstracts KW - Risk assessment KW - Neurodevelopmental disorders KW - Birth weight KW - Age KW - Dysplasia KW - Head KW - Necrotizing enterocolitis KW - Steroid hormones KW - Development KW - Sepsis KW - Cognitive ability KW - Risk factors KW - Regression analysis KW - Neonates KW - periventricular leukomalacia KW - Infants KW - N3 11003:Developmental neuroscience KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19412312?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pediatrics&rft.atitle=Neurodevelopmental+and+Growth+Outcomes+of+Extremely+Low+Birth+Weight+Infants+After+Necrotizing+Enterocolitis&rft.au=Hintz%2C+Susan+R%3BKendrick%2C+Douglas+E%3BStoll%2C+Barbara+J%3BVohr%2C+Betty+R%3BFanaroff%2C+Avroy+A%3BDonovan%2C+Edward+F%3BPoole%2C+WKenneth%3BBlakely%2C+Martin+L%3BWright%2C+Linda%3BHiggins%2C+Rosemary&rft.aulast=Hintz&rft.aufirst=Susan&rft.date=2005-03-01&rft.volume=115&rft.issue=3&rft.spage=696&rft.isbn=&rft.btitle=&rft.title=Pediatrics&rft.issn=00314005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Neurodevelopmental disorders; Risk assessment; Birth weight; Age; Head; Dysplasia; Necrotizing enterocolitis; Development; Steroid hormones; Sepsis; Cognitive ability; Risk factors; Regression analysis; Neonates; Infants; periventricular leukomalacia ER - TY - JOUR T1 - Improvement of Motor Function with Noninvasive Cortical Stimulation in a Patient with Chronic Stroke AN - 19409002; 6176653 AB - This manuscript reports the effects of transcranial DC stimulation (tDCS), a technique that enhances cortical plasticity in healthy humans, on motor function in a patient with chronic subcortical ischemic stroke. tDCS but not sham applied in a double-blind protocol to motor regions of the affected hemisphere led to improvements in pinch force, Jebsen-Taylor Hand Function Test, and simple reaction times in the paretic hand that outlasted the stimulation period for at least 40 min. These changes were accompanied by increased corticomotor excitability identified by enhanced recruitment curves and reduced intracortical inhibition to transcranial magnetic stimulation. These results document a beneficial effect of noninvasive brain stimulation on motor function in a human patient with stroke and raise the hypothesis of its potential application in neurorehabilitation. JF - Neurorehabilitation and Neural Repair AU - Hummel, Friedhelm AU - Cohen, Leonardo G AD - Human Cortical Physiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, Cortical Physiology Research Group, Department of Neurology, and Hertie Institute for Clinical Brain Research, Eberhard-Karls University Tuebingen, Tuebingen, Germany Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 14 EP - 19 PB - Sage Publications, Inc., 2455 Teller Road Thousand Oaks CA 91320 USA, [mailto:info@sagepub.com], [URL:http://www.sagepub.com/] VL - 19 IS - 1 SN - 1545-9683, 1545-9683 KW - Physical Education Index; CSA Neurosciences Abstracts KW - Hands KW - Recruitment KW - Plasticity (cortical) KW - Stroke KW - Brain KW - Hand KW - Motor performance tests KW - Techniques KW - Patients KW - Health KW - Ischemia KW - Excitability KW - Inhibition KW - Reaction time KW - Transcranial magnetic stimulation KW - Cortex KW - Reaction time task KW - Stimuli KW - PE 110:Physical Therapy KW - N3 11027:Neurology & neuropathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19409002?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurorehabilitation+and+Neural+Repair&rft.atitle=Improvement+of+Motor+Function+with+Noninvasive+Cortical+Stimulation+in+a+Patient+with+Chronic+Stroke&rft.au=Hummel%2C+Friedhelm%3BCohen%2C+Leonardo+G&rft.aulast=Hummel&rft.aufirst=Friedhelm&rft.date=2005-03-01&rft.volume=19&rft.issue=1&rft.spage=14&rft.isbn=&rft.btitle=&rft.title=Neurorehabilitation+and+Neural+Repair&rft.issn=15459683&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Reaction time; Hands; Stroke; Brain; Techniques; Motor performance tests; Stimuli; Health; Patients; Inhibition; Transcranial magnetic stimulation; Cortex; Reaction time task; Plasticity (cortical); Recruitment; Hand; Ischemia; Excitability ER - TY - JOUR T1 - Manganese-enhanced magnetic resonance imaging of mouse brain after systemic administration of MnCl sub(2): Dose-dependent and temporal evolution of T sub(1) contrast AN - 19408111; 6499405 AB - Manganese is a useful contrast agent for MRI of animals. Previously, it has been shown that systemic doses of MnCl sub(2) provide unique contrast in the rodent brain, enabling visualization of neuroarchitecture. The present work investigates the dose and temporal dependence of brain enhancement after i.v. administration of MnCl sub(2). Varying doses of MnCl sub(2) (9-175 mg/kg) were administered to mice from 0 to 24 h prior to T sub(1)-weighted manganese-enhanced MRI (MEMRI) at 11.7 T. Pre-MnCl sub(2) T sub(1) values measured in different brain regions ranged from 1.17 plus or minus 0.03 to 1.76 plus or minus 0.01 s. Post-MnCl sub(2) T sub(1) measured 24 hr after administration of MnCl sub(2) were significantly decreased, even after the lowest dose of MnCl sub(2). The largest decreases occurred in the pituitary gland, where post-MnCl sub(2) T sub(1) ranged from 231 plus or minus 23 ms following the lowest dose to 143 plus or minus 43 ms after the highest dose, while the smallest decreases were observed in cortex (post-MnCl sub(2) T sub(1) = 1060 plus or minus 5 ms for low dose and 637 plus or minus 5 ms for high dose). The contrast resulting after 14 hr did not change up to 24 hr. Enhancement first occurred in subarachnoid spaces, followed by ventricles and periventricular tissues, and finally reached the remainder of the brain. Cortical layers were detected at higher doses (>88 mg/kg) and olfactory bulb layers were detected with the lowest dose (9 mg/kg). Temporal evolution of the enhancement of the olfactory bulb layers was observed. In some regions of the brain, such as hippocampus and thalamus, the changes in contrast detected between 2 and 14 hr used very specific pathways. These results demonstrate that both the dose and the time after MnCl sub(2) can be manipulated to optimize brain contrast in a region-specific manner. JF - Magnetic Resonance in Medicine AU - Lee, Jung Hee AU - Silva, Afonso C AU - Merkle, Hellmut AU - Koretsky, Alan P AD - Laboratory of Functional and Molecular Imaging, National Institutes of Neurological Disorders and Stroke, 10 Center Drive, Building 10 Room B1D118, Bethesda, MD 20892-1065, USA, KoretskyA@ninds.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 640 EP - 648 PB - John Wiley & Sons, Ltd. VL - 53 IS - 3 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Hippocampus KW - Magnetic resonance imaging KW - Brain KW - subarachnoid space KW - Thalamus KW - Olfactory bulb KW - Cortex KW - Pituitary KW - Contrast media KW - N.M.R. KW - Manganese KW - Evolution KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19408111?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Manganese-enhanced+magnetic+resonance+imaging+of+mouse+brain+after+systemic+administration+of+MnCl+sub%282%29%3A+Dose-dependent+and+temporal+evolution+of+T+sub%281%29+contrast&rft.au=Lee%2C+Jung+Hee%3BSilva%2C+Afonso+C%3BMerkle%2C+Hellmut%3BKoretsky%2C+Alan+P&rft.aulast=Lee&rft.aufirst=Jung&rft.date=2005-03-01&rft.volume=53&rft.issue=3&rft.spage=640&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20368 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Brain; Magnetic resonance imaging; Evolution; Olfactory bulb; Cortex; Hippocampus; Neuroimaging; Thalamus; Pituitary; N.M.R.; subarachnoid space; Manganese; Contrast media DO - http://dx.doi.org/10.1002/mrm.20368 ER - TY - JOUR T1 - Montanide super([registeredsign]) ISA 720 vaccines: quality control of emulsions, stability of formulated antigens, and comparative immunogenicity of vaccine formulations AN - 17863641; 6202451 AB - Montanide super([registeredsign]) ISA 720 is an experimental adjuvant, formulated as water-in-oil emulsions, that induces high antibody titers in several animal species. It has been used in human vaccine trials with malaria and HIV vaccines. The heightened response is likely due, in part, to the formation of a depot at the injection site. However, post-formulation modifications were seen with seven proteins tested during storage of ISA 720 formulations at 37 C for 1 week and two proteins stored longer at 4 C. Potency studies in mice, in which the stored vaccines were diluted into placebo emulsions for appropriate dosing, indicated that this instability could lead to loss of immunogenicity in the post-injection depot, limiting the allowable storage time of preformed vaccines. We describe point-of-injection formulation for ISA 720 vaccines that meets the requirement for in vitro stability. For preformed vaccines, addition of glycine or glycylglycine prevented antigen modification on storage at 37 C, providing a potential way of stabilizing antigen/ISA 720 formulations for in vitro storage and the post-injection depot. JF - Vaccine AU - Miles, A P AU - McClellan, HA AU - Rausch, K M AU - Zhu, D AU - Whitmore, MD AU - Singh, S AU - Martin, L B AU - Wu, Y AU - Giersing, B K AU - Stowers, A W AU - Long, CA AU - Saul, A AD - Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 5640 Fishers Lane, Twinbrook I Room 1118, Rockville, MD 20852, USA, amiles@niaid.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 2530 EP - 2539 PB - Butterworth-Heinemann, 313 Washington St. Newton MA 02158 USA VL - 23 IS - 19 SN - 0264-410X, 0264-410X KW - HIV KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - Glycine KW - Malaria KW - Adjuvants KW - Antibodies KW - Immunogenicity KW - Human immunodeficiency virus KW - Quality control KW - Vaccines KW - W3 33365:Vaccines (other) KW - F 06807:Active immunization KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17863641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Vaccine&rft.atitle=Montanide+super%28%5Bregisteredsign%5D%29+ISA+720+vaccines%3A+quality+control+of+emulsions%2C+stability+of+formulated+antigens%2C+and+comparative+immunogenicity+of+vaccine+formulations&rft.au=Miles%2C+A+P%3BMcClellan%2C+HA%3BRausch%2C+K+M%3BZhu%2C+D%3BWhitmore%2C+MD%3BSingh%2C+S%3BMartin%2C+L+B%3BWu%2C+Y%3BGiersing%2C+B+K%3BStowers%2C+A+W%3BLong%2C+CA%3BSaul%2C+A&rft.aulast=Miles&rft.aufirst=A&rft.date=2005-03-01&rft.volume=23&rft.issue=19&rft.spage=2530&rft.isbn=&rft.btitle=&rft.title=Vaccine&rft.issn=0264410X&rft_id=info:doi/10.1016%2Fj.vaccine.2004.08.049 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Human immunodeficiency virus; Vaccines; Immunogenicity; Glycine; Antibodies; Malaria; Adjuvants; Quality control DO - http://dx.doi.org/10.1016/j.vaccine.2004.08.049 ER - TY - JOUR T1 - Factors Characterizing Staphylococcus epidermidis Invasiveness Determined by Comparative Genomics AN - 17853618; 6169388 AB - Virulence mechanisms of the leading nosocomial pathogen Staphylococcus epidermidis are poorly understood. We used microarray-based genome-wide comparison of clinical and commensal S. epidermidis strains to identify putative virulence determinants. Our study revealed high genetic variability of the S. epidermidis genome, new markers for invasiveness of S. epidermidis, and potential targets for drug development against S. epidermidis infections. JF - Infection and Immunity AU - Yao, Yufeng AU - Sturdevant, Daniel E AU - Villaruz, Amer AU - Xu, Lin AU - Gao, Qian AU - Otto, Michael AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, The National Institutes of Health, Hamilton, Montana. Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 1856 EP - 1860 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 3 SN - 0019-9567, 0019-9567 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Virulence KW - Invasiveness KW - Commensals KW - Genetic diversity KW - Drug development KW - Pathogens KW - genomics KW - Infection KW - Staphylococcus epidermidis KW - Lead KW - G 07320:Bacterial genetics KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17853618?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Factors+Characterizing+Staphylococcus+epidermidis+Invasiveness+Determined+by+Comparative+Genomics&rft.au=Yao%2C+Yufeng%3BSturdevant%2C+Daniel+E%3BVillaruz%2C+Amer%3BXu%2C+Lin%3BGao%2C+Qian%3BOtto%2C+Michael&rft.aulast=Yao&rft.aufirst=Yufeng&rft.date=2005-03-01&rft.volume=73&rft.issue=3&rft.spage=1856&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Virulence; Invasiveness; Commensals; Genetic diversity; Drug development; genomics; Pathogens; Infection; Lead; Staphylococcus epidermidis ER - TY - JOUR T1 - Digital biology: an emerging and promising discipline AN - 17837347; 6213464 AB - This article examines the role of computation and quantitative methods in modern biomedical research to identify emerging scientific, technical, policy and organizational trends. It identifies common concerns and practices in the emerging community of computationally-oriented bio-scientists by reviewing a national symposium, Digital Biology: the Emerging Paradigm, held at the National Institutes of Health in Bethesda, Maryland, November 6 super(t) super(h) and 7 super(t) super(h) 2003. This meeting showed how biomedical computing promises scientific breakthroughs that will yield significant health benefits. Three key areas that define the emerging discipline of digital biology are: scientific data integration, multi-scale modeling and networked science. Each area faces unique technical challenges and information policy issues that must be addressed as the field matures. Here we summarize the emergent challenges and offer suggestions to academia, industry and government on how best to expand the role of computation in their scientific activities. JF - Trends in Biotechnology AU - Morris, R W AU - Bean, CA AU - Farber, G K AU - Gallahan, D AU - Jakobsson, E AU - Liu, Y AU - Lyster, P M AU - Peng, GCY AU - Roberts, F S AU - Twery, M AU - Whitmarsh, J AU - Skinner, K Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 113 EP - 117 VL - 23 IS - 3 SN - 0167-7799, 0167-7799 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Reviews KW - Computer applications KW - Data acquisition KW - W4 140:Bioinformatics & Computers in Health & Medicine KW - W3 33080:Bioinformatics and computer applications KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17837347?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Trends+in+Biotechnology&rft.atitle=Digital+biology%3A+an+emerging+and+promising+discipline&rft.au=Morris%2C+R+W%3BBean%2C+CA%3BFarber%2C+G+K%3BGallahan%2C+D%3BJakobsson%2C+E%3BLiu%2C+Y%3BLyster%2C+P+M%3BPeng%2C+GCY%3BRoberts%2C+F+S%3BTwery%2C+M%3BWhitmarsh%2C+J%3BSkinner%2C+K&rft.aulast=Morris&rft.aufirst=R&rft.date=2005-03-01&rft.volume=23&rft.issue=3&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Trends+in+Biotechnology&rft.issn=01677799&rft_id=info:doi/10.1016%2Fj.tibtech.2005.01.005 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Reviews; Data acquisition; Computer applications DO - http://dx.doi.org/10.1016/j.tibtech.2005.01.005 ER - TY - JOUR T1 - Unique patterns of gene expression changes in liver after treatment of mice for 2 weeks with different known carcinogens and non-carcinogens AN - 17836219; 6167264 AB - Previously we demonstrated that the mouse liver tumor response to the non- genotoxic carcinogens oxazepam and Wyeth-14,643 involved more differences than similarities in changes in early gene expression. In this study we used quantitative real-time PCR and oligonucleotide microarray analysis to identify genes that were up-or down-regulated in mouse liver early after treatment with different known carcinogens, including oxazepam (125 and 2500 p.p.m.), o- nitrotoluene (1250 and 5000 p.p.m.) and methyleugenol (75 mg/kg/day), or the non-carcinogens p-nitrotoluene (5000 p.p.m.), eugenol (75 mg/kg/day) and acetaminophen (6000 p.p.m.). Starting at 6 weeks of age, mice were treated with the different compounds for 2 weeks in the diet, at which time the livers were collected. First, expression of 12 genes found previously to be altered in liver after 2 weeks treatment with oxazepam and/or Wyeth-14,643 was examined in livers from the various chemical treatment groups. These gene expression changes were confirmed for the livers from the oxazepam-treated mice in the present study, but were not good early markers for all the carcinogens in this study. In addition, expression of 20 842 genes was assessed by oligonucleotide microarray [n = 4 livers/group, 2 hybridizations/liver (with fluor reversals)] and the results were analyzed using the Rosetta Resolver System and GeneSpring software. The analyses revealed that several cancer-related genes, including Fhit, Wwox, Tsc-22 and Gadd45b, were induced or repressed in unique patterns for specific carcinogens and not altered by the non-carcinogens. The data indicate that even if the tumor response, including molecular alterations, is similar, such as for oxazepam and methyleugenol, early gene expression changes appear to be carcinogen specific and seem to involve apoptosis and cell cycle-related genes. JF - Carcinogenesis AU - Iida, Mari AU - Anna, Colleen H AU - Holliday, Wanda M AU - Collins, Jennifer B AU - Cunningham, Michael L AU - Sills, Robert C AU - Devereux, Theodora R AD - Laboratory of Molecular Carcinogenesis, National Center for Toxicogenomics, Laboratory of Pharmacology and Chemistry and Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, NC Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 689 EP - 699 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - Mice KW - Wyeth-14,643 KW - methyleugenol KW - oxazepam KW - Toxicology Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Diets KW - Apoptosis KW - Genotoxicity KW - Cell cycle KW - Carcinogens KW - Oligonucleotides KW - Gene expression KW - Computer programs KW - Carcinogenesis KW - Liver KW - Polymerase chain reaction KW - eugenol KW - Acetaminophen KW - X 24240:Miscellaneous KW - N 14010:Physical & Computer Methods & Assays UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17836219?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Unique+patterns+of+gene+expression+changes+in+liver+after+treatment+of+mice+for+2+weeks+with+different+known+carcinogens+and+non-carcinogens&rft.au=Iida%2C+Mari%3BAnna%2C+Colleen+H%3BHolliday%2C+Wanda+M%3BCollins%2C+Jennifer+B%3BCunningham%2C+Michael+L%3BSills%2C+Robert+C%3BDevereux%2C+Theodora+R&rft.aulast=Iida&rft.aufirst=Mari&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=689&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Diets; Apoptosis; Cell cycle; Genotoxicity; Carcinogens; Oligonucleotides; Gene expression; Computer programs; Carcinogenesis; Liver; eugenol; Polymerase chain reaction; Acetaminophen ER - TY - JOUR T1 - Identification of Mycobacterium Species by secA1 Sequences AN - 17830701; 6190279 AB - We describe a novel molecular method for the differentiation and identification of 29 mycobacterial species. The target is the secA1 gene that codes for the essential protein SecA1, a key component of the major pathway of protein secretion across the cytoplasmic membrane. A 700-bp region of the secA1 gene was amplified and sequenced from 47 American Type Culture Collection strains of 29 Mycobacterium species as well as from 59 clinical isolates. Sequence variability in the amplified segment of the secA1 gene allowed the differentiation of all species except for the members of the Mycobacterium tuberculosis (MTB) complex, which had identical sequences. A range of 83.3 to 100% interspecies similarity was observed. All species could also be differentiated by their amino acid sequences as deduced from the sequenced region of the secA1 gene, with the exception of the MTB complex. Partial sequences of secA1 from clinical isolates belonging to nine frequently isolated species of mycobacteria revealed a very high intraspecies similarity at the DNA level (typically >99%; range, 96.0 to 100%); all clinical isolates were correctly identified. Comparison of the deduced 233-amino-acid sequences among clinical isolates of the same species showed between 99.6 and 100% similarity. To our knowledge, this is the first time a secretion-related gene has been used for the identification of the species within a bacterial genus. JF - Journal of Clinical Microbiology AU - Zelazny, Adrian M AU - Calhoun, Leslie B AU - Li, Li AU - Shea, Yvonne R AU - Fischer, Steven H AD - Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 1051 EP - 1058 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 43 IS - 3 SN - 0095-1137, 0095-1137 KW - SecA1 protein KW - Microbiology Abstracts B: Bacteriology KW - Clinical isolates KW - Differentiation KW - Amino acids KW - Nucleotide sequence KW - Secretion KW - Cytoplasmic membranes KW - DNA KW - American Type Culture Collection KW - Mycobacterium tuberculosis KW - J 02710:Identification, taxonomy and typing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17830701?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Clinical+Microbiology&rft.atitle=Identification+of+Mycobacterium+Species+by+secA1+Sequences&rft.au=Zelazny%2C+Adrian+M%3BCalhoun%2C+Leslie+B%3BLi%2C+Li%3BShea%2C+Yvonne+R%3BFischer%2C+Steven+H&rft.aulast=Zelazny&rft.aufirst=Adrian&rft.date=2005-03-01&rft.volume=43&rft.issue=3&rft.spage=1051&rft.isbn=&rft.btitle=&rft.title=Journal+of+Clinical+Microbiology&rft.issn=00951137&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Mycobacterium tuberculosis; Clinical isolates; Differentiation; Secretion; American Type Culture Collection; Amino acids; DNA; Cytoplasmic membranes; Nucleotide sequence ER - TY - JOUR T1 - The Validation of a Pesticide Exposure Algorithm Using Biological Monitoring Results AN - 17809414; 6220416 AB - A pesticide exposure algorithm was developed to calculate pesticide exposure intensity scores based on responses to questions about pesticide handling procedures and application methods in a self-administered questionnaire. The validity of the algorithm was evaluated through comparison of the algorithm scores with biological monitoring data from a study of 126 pesticide applicators who applied the herbicdes MCPA or 2,4-D. The variability in the algorithm scores calculated for these applicators was due primarily to differences in their use of personal protective equipment (PPE). Rubber gloves were worn by 75% of applicators when mixing and 22% when applying pesticides, rubber boots were worn by 33% when mixing and 23% when applying, and goggles were worn by 33% and 17% of applicators when mixing and when applying, respectively. Only 2% of applicators wore all three types of PPE when both mixing and applying, and 15% wore none of these three types of PPE when either mixing or applying. Substantial variability was also observed in the concentrations of pesticides detected in the post application urine samples. The concentration of MCPA detected in urine samples collected on the second day after the application ranged from less than <1.0 to 610 mu g/L among 84 of the applicators who applied MCPA. The concentrations of 2,4-D detected in the urine samples ranged from less than <1.0 to 514 mu g/L among 41 of the applicators who applied 2,4-D. When categorized into three groups based on the algorithm scores, the geometric mean in the highest exposure group was 20 mu g/L compared with 5 mu g/L in the lowest exposure group for the MCPA applicators, and 29 mu g/L in highest exposure group compared with 2 mu g/L in the low exposure group for the 2,4-D applicators. A regression analysis detected statistically significant trends in the geometric mean of the urine concentrations across the exposure categories for both the 2,4-D and the MCPA applicators. The algorithm scores, based primarily on the use of PPE, appear to provide a reasonably valid measure of exposure intensity for these applicators, however, further studies are needed to generalize these results to other types of pesticides and application methods. JF - Journal of Occupational and Environmental Hygiene AU - Coble, J AU - Arbuckle, T AU - Lee, Wonjin AU - Alavanja, M AU - Dosemeci, M AD - Occupational and Environmental Epidemiology Branch, Department of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd., EPQ 8110, Rockville, MD 20892-7240, USA, jcoble@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 194 EP - 201 VL - 2 IS - 3 SN - 1545-9624, 1545-9624 KW - Health & Safety Science Abstracts; Toxicology Abstracts KW - 2,4-D KW - Bioindicators KW - Algorithms KW - gloves KW - biomarkers KW - Protective clothing KW - Urine KW - Pesticides KW - Gloves KW - 2,4-Dichlorophenoxyacetic acid KW - Occupational exposure KW - X 24222:Analytical procedures KW - H 5000:Pesticides UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17809414?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Occupational+and+Environmental+Hygiene&rft.atitle=The+Validation+of+a+Pesticide+Exposure+Algorithm+Using+Biological+Monitoring+Results&rft.au=Coble%2C+J%3BArbuckle%2C+T%3BLee%2C+Wonjin%3BAlavanja%2C+M%3BDosemeci%2C+M&rft.aulast=Coble&rft.aufirst=J&rft.date=2005-03-01&rft.volume=2&rft.issue=3&rft.spage=194&rft.isbn=&rft.btitle=&rft.title=Journal+of+Occupational+and+Environmental+Hygiene&rft.issn=15459624&rft_id=info:doi/10.1080%2F15459620590923343 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Pesticides; 2,4-Dichlorophenoxyacetic acid; Occupational exposure; Urine; Protective clothing; gloves; Bioindicators; Algorithms; 2,4-D; Gloves; biomarkers DO - http://dx.doi.org/10.1080/15459620590923343 ER - TY - JOUR T1 - Induction of preneoplastic lung lesions in guinea pigs by cigarette smoke inhalation and their exacerbation by high dietary levels of vitamins C and E AN - 17805750; 6167240 AB - The development of effective chemopreventive agents against cigarette smoke- induced lung cancer could be greatly facilitated by the availability of suitable laboratory animal models. Here we report that male Hartley guinea pigs treated with cigarette smoke by inhalation twice a day for 28 days developed preneoplastic lung lesions, including bronchial hyperplasia, dysplasia and squamous metaplasia, analogous to those found in human smokers. The lesions were accompanied by increased expression of proliferating cell nuclear antigen and activation of the serine/threonine kinase Akt in the bronchial epithelium. In contrast, no lung lesions were found in guinea pigs ('sham smoked') that were submitted to identical procedures but without cigarettes. Compared with a diet low in vitamin C (50 p.p.m.) and vitamin E (15 p.p.m.), a diet high in vitamin C (4000 p.p.m.) and vitamin E (40 p.p.m.) significantly increased the incidence of these lesions. The inclusion of 1,4-phenylenebis(methylene)selenocyanate (p- XSC), a synthetic chemopreventive organoselenium compound, in the high vitamin C-high vitamin E diet at a level of 15 p.p.m. as selenium appeared to decrease the lesion incidence. Administration of (-)-epigallocatechin gallate, a powerful green tea polyphenolic antioxidant, at 560 p.p.m. in the drinking water had no effect. As in human smokers, levels of ascorbate in blood plasma, lung, liver and the adrenal glands were significantly decreased by cigarette smoke inhalation. These results identify a relevant in vivo laboratory model of cigarette smoke-induced lung cancer, suggest that p-XSC may have activity as a chemopreventive agent against cigarette smoke-induced lung lesions and provide additional evidence that very high dietary levels of certain antioxidants can have co-carcinogenic activity in cigarette smoke-induced lung cancer. JF - Carcinogenesis AU - Fiala, Emerich S AU - Sohn, Ock Soon AU - Wang, Chung-Xiou AU - Seibert, Eleanore AU - Tsurutani, Junji AU - Dennis, Phillip A AU - El-Bayoumy, Karam AU - Sodum, Rama S AU - Desai, Dhimant AU - Reinhardt, Joel AU - Aliaga, Cesar AD - Institute for Cancer Prevention, Valhalla, NY, USA and Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 605 EP - 612 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 26 IS - 3 SN - 0143-3334, 0143-3334 KW - 1,4-phenylenebis(methylene)selenocyanate KW - guinea pigs KW - Toxicology Abstracts KW - Inhalation KW - Diets KW - Antioxidants KW - Protein-serine/threonine kinase KW - green tea KW - Laboratory animals KW - Cigarette smoke KW - Proliferating cell nuclear antigen KW - epigallocatechin gallate KW - Ascorbic acid KW - Hyperplasia KW - Vitamin E KW - Metaplasia KW - Vitamins KW - Carcinogenesis KW - AKT protein KW - chemopreventive agents KW - Drinking water KW - Lung cancer KW - X 24120:Food, additives & contaminants KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17805750?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Induction+of+preneoplastic+lung+lesions+in+guinea+pigs+by+cigarette+smoke+inhalation+and+their+exacerbation+by+high+dietary+levels+of+vitamins+C+and+E&rft.au=Fiala%2C+Emerich+S%3BSohn%2C+Ock+Soon%3BWang%2C+Chung-Xiou%3BSeibert%2C+Eleanore%3BTsurutani%2C+Junji%3BDennis%2C+Phillip+A%3BEl-Bayoumy%2C+Karam%3BSodum%2C+Rama+S%3BDesai%2C+Dhimant%3BReinhardt%2C+Joel%3BAliaga%2C+Cesar&rft.aulast=Fiala&rft.aufirst=Emerich&rft.date=2005-03-01&rft.volume=26&rft.issue=3&rft.spage=605&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Ascorbic acid; Vitamin E; Diets; Lung cancer; Inhalation; Cigarette smoke; chemopreventive agents; Antioxidants; Vitamins; Drinking water; Hyperplasia; Proliferating cell nuclear antigen; AKT protein; Protein-serine/threonine kinase; Laboratory animals; Carcinogenesis; Metaplasia; epigallocatechin gallate; green tea ER - TY - JOUR T1 - Apoptotic volume decrease and nitric oxide AN - 17774382; 6149262 AB - Apoptosis is a physiological cell death process whose well-defined characteristics distinguish it from more accidental cell death processes. The loss of cell volume, or cell shrinkage, recently termed apoptotic volume decrease (AVD), is considered a hallmark of the apoptotic process. The activation and/or repression of the AVD process has been shown to be quite complex during apoptosis, with the involvement of multiple ionic transport mechanisms acting in both a cell type and stimulus specific manner. Similarly, the role of nitric oxide (NO) during apoptosis has also been shown to be just as complex, specifically in its ability to either induce and/or prevent apoptosis. This review examines current evidence for a link between AVD and NO and how they may interact during the programmed cell death process. JF - Toxicology AU - Bortner, C D AD - The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health, Research Triangle Park, NC 27709, USA, bortner@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 213 EP - 221 PB - Elsevier Science Ireland Ltd., P.O. Box 85 Limerick Ireland VL - 208 IS - 2 SN - 0300-483X, 0300-483X KW - Toxicology Abstracts KW - Cell death KW - Apoptosis KW - Reviews KW - Cell size KW - Atrophy KW - Nitric oxide KW - X 24250:Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17774382?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=Apoptotic+volume+decrease+and+nitric+oxide&rft.au=Bortner%2C+C+D&rft.aulast=Bortner&rft.aufirst=C&rft.date=2005-03-01&rft.volume=208&rft.issue=2&rft.spage=213&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/10.1016%2Fj.tox.2004.11.024 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Apoptosis; Cell death; Nitric oxide; Reviews; Cell size; Atrophy DO - http://dx.doi.org/10.1016/j.tox.2004.11.024 ER - TY - JOUR T1 - Synergistic Production of Lung Free Radicals by Diesel Exhaust Particles and Endotoxin AN - 17545499; 6433543 AB - The present study tested the hypothesis that free radicals were involved in the pathogenesis of lung injury caused by diesel exhaust particles (DEP) and bacterial lipopolysaccharides (LPS). Intratracheal coinstillation of DEP and LPS in rat lungs resulted in synergistic enhancement of free radical generation in the lungs. The radical metabolites were characterized as lipid-derived by electron spin resonance (ESR). The free radical generation was paralleled by a synergistic increase in total protein and by infiltration of neutrophils in the bronchoalveolar lavage (BAL) fluid of the lungs. Experiments with NADP-reduced (NADPH) oxidase and iNOS knockout mice showed that NADPH oxidase and iNOS did not contribute to free radical generation. However, pretreatment with the macrophage toxicant GdCl sub(3), the xanthine oxidase (XO) inhibitor allopurinol, and the Fe super(III) chelator Desferal resulted in a marked decrease in free radical generation, lung inflammation, and lung injury. These effects were concomitant with the inhibition of XO activity in BAL, suggesting that the activated macrophages and the activity of XO contributed to the generation of free radicals caused by DEP and LPS. This is the first demonstration that DEP and LPS work synergistically to enhance free radical generation in lungs, mediated by the activation of local XO. JF - American Journal of Respiratory and Critical Care Medicine AU - Arimoto, Toyoko AU - Kadiiska, M B AU - Sato, Keizo AU - Corbett, J AU - Mason, R P AD - Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health (NIEHS/NIH), Research Triangle Park, NC 27709, USA, mason4@niehs.nih.gov Y1 - 2005/03/01/ PY - 2005 DA - 2005 Mar 01 SP - 379 EP - 387 VL - 171 IS - 5 SN - 0003-0805, 0003-0805 KW - Toxicology Abstracts KW - Macrophages KW - Endotoxins KW - Injuries KW - Toxicants KW - Free radicals KW - Leukocytes (neutrophilic) KW - Metabolites KW - Alveoli KW - Exhausts KW - Inflammation KW - Nitric-oxide synthase KW - allopurinol KW - Bronchus KW - Lung KW - Xanthine oxidase KW - Lipopolysaccharides KW - Diesel KW - Trachea KW - NADPH oxidase KW - Radicals KW - X 24171:Microbial UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17545499?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Respiratory+and+Critical+Care+Medicine&rft.atitle=Synergistic+Production+of+Lung+Free+Radicals+by+Diesel+Exhaust+Particles+and+Endotoxin&rft.au=Arimoto%2C+Toyoko%3BKadiiska%2C+M+B%3BSato%2C+Keizo%3BCorbett%2C+J%3BMason%2C+R+P&rft.aulast=Arimoto&rft.aufirst=Toyoko&rft.date=2005-03-01&rft.volume=171&rft.issue=5&rft.spage=379&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Respiratory+and+Critical+Care+Medicine&rft.issn=00030805&rft_id=info:doi/10.1164%2Frccm.200402-248OC LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Endotoxins; Macrophages; Toxicants; Injuries; Free radicals; Leukocytes (neutrophilic); Metabolites; Alveoli; Inflammation; Exhausts; Nitric-oxide synthase; allopurinol; Bronchus; Lung; Xanthine oxidase; Lipopolysaccharides; Diesel; Trachea; Radicals; NADPH oxidase DO - http://dx.doi.org/10.1164/rccm.200402-248OC ER - TY - JOUR T1 - Image informatics at a national research center AN - 17524411; 6201669 AB - Image informatics at the Communications Engineering Branch of the Lister Hill National Center for Biomedical Communications (LHNCBC), an R&D division of the National Library of Medicine (NLM), includes document and biomedical images. In both domains, research into computer-assisted methods for information extraction, and the implementation of prototype systems incorporating such methods, is central to our mission. Current document image research focuses on extracting bibliographic data from scanned journal articles. Current biomedical imaging work focuses on content-based image retrieval (CBIR) and related problems in segmentation, indexing, and classifying collections of images of the spine and of the uterine cervix. JF - Computerized Medical Imaging and Graphics AU - Long, L R AU - Antani, S K AU - Thoma, G R AD - Department of Health and Human Services, Communications Engineering Branch, US National Library of Medicine, Lister Hill National Center for Biomedical Communications, National Institutes of Health, 8600 Rockville Pike, Bldg 38A MS 55, Bethesda, MD 20894, USA, rlong@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 171 EP - 193 PB - Pergamon Press Inc., 660 White Plains Rd., Floor 2 Tarrytown NY 10591-5153 USA VL - 29 IS - 2-3 SN - 0895-6111, 0895-6111 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Uterus KW - Spine KW - Segmentation KW - Bioinformatics KW - Cervix KW - imaging KW - W4 150:Medical Imaging KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17524411?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Computerized+Medical+Imaging+and+Graphics&rft.atitle=Image+informatics+at+a+national+research+center&rft.au=Long%2C+L+R%3BAntani%2C+S+K%3BThoma%2C+G+R&rft.aulast=Long&rft.aufirst=L&rft.date=2005-03-01&rft.volume=29&rft.issue=2-3&rft.spage=171&rft.isbn=&rft.btitle=&rft.title=Computerized+Medical+Imaging+and+Graphics&rft.issn=08956111&rft_id=info:doi/10.1016%2Fj.compmedimag.2004.09.015 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cervix; Segmentation; Spine; imaging; Uterus; Bioinformatics DO - http://dx.doi.org/10.1016/j.compmedimag.2004.09.015 ER - TY - JOUR T1 - BM stem cells and cardiac repair: where do we stand in 2004? AN - 17468694; 6654769 AB - Adult BM stem cells are being investigated for their potential to regenerate injured tissues by a process referred to as plasticity or transdifferentiation. Although data supporting stem cell plasticity is extensive, a controversy has emerged based on findings that propose cell-cell fusion as a more appropriate interpretation for this phenomenon. A major focus of this controversy is the claim that acutely infarcted myocardium in adult hearts can be regenerated by BM stem cells. Many researchers consider the adult heart to be a post-mitotic organ, whereas others believe that a low level of cardiomyocyte renewal occurs throughout life. If renewal occurs, it may be in response to cardiac stem cell activity or to stem cells that migrate from distant tissues. Post-mortem microscopic analysis of experimentally induced myocardial infarctions in several rodent models suggests that cardiomyocyte renewal is achieved by stem cells that infiltrate the damaged tissue. For a better understanding of the possible involvement of stem cells in myocardial regeneration, it is important to develop appropriate technologies to monitor myocardial repair over time with an emphasis on large animal models. Studies on non-human primate, swine and canine models of acute myocardial infarctions would enable investigators to utilize clinical quality cell-delivery devices, track labeled donor cells after precision transplantation and utilize non-invasive imaging for functional assays over time with clinical accuracy. In addition, if stem cell plasticity is to reach the next level of acceptance, it is important to identify the environmental cues needed for stem cell trafficking and to define the genetic and cellular mechanisms that initiate transdifferentiation. Only then will it be possible to determine if, and to what extent, BM stem cells are involved in myocardial regeneration and to begin to regulate precisely tissue repair. JF - Cytotherapy AU - Orlic, D AD - Cardiovascular Branch National Heart Lung and Blood Institute, NIH Bethesda Maryland USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 3 EP - 15 VL - 7 IS - 1 SN - 1465-3249, 1465-3249 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Heart KW - Donors KW - Stem cells KW - Animal models KW - cardiomyocytes KW - imaging KW - Cell fusion KW - Myocardial infarction KW - Myocardium KW - Plasticity (functional) KW - W3 33170:Cellular based KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17468694?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cytotherapy&rft.atitle=BM+stem+cells+and+cardiac+repair%3A+where+do+we+stand+in+2004%3F&rft.au=Orlic%2C+D&rft.aulast=Orlic&rft.aufirst=D&rft.date=2005-03-01&rft.volume=7&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Cytotherapy&rft.issn=14653249&rft_id=info:doi/10.1080%2F14653240510018028 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Stem cells; Heart; Animal models; Myocardial infarction; cardiomyocytes; imaging; Cell fusion; Plasticity (functional); Donors; Myocardium DO - http://dx.doi.org/10.1080/14653240510018028 ER - TY - JOUR T1 - Review Article Toxic Oil Syndrome: Review of Immune Aspects of the Disease AN - 17436098; 6585582 AB - In 1981-1982, individuals in fourteen central and northwest provinces in Spain were affected by an illness that was eventually labeled toxic oil syndrome (TOS) by the World Health Organization. Thousands of individuals were diagnosed with, and 356 people eventually died from, the disease. The disease shares striking similarities with several autoimmune diseases, particularly eosinophilia-myalgia syndrome (EMS) and diffuse fasciitis with eosinophilia (DFE). As with many other autoimmune diseases, women were more severely affected than men and made up a significant portion of TOS-related deaths. While a number of etiologic agents were investigated, disease occurrence was found to be significantly associated with consumption of contaminated rapeseed oil produced by a particular refinery. Two compounds, 1,2-di-oleyl ester (DEPAP) and oleic anilide are considered to be biologically relevant contaminants that may contribute to disease development. Toxic oil syndrome was a three-phase disease with an initial non-necrotizing vasculitis in multiple organs. Suspected immune mechanisms in TOS include activation of T-cells, altered cytokine production, and several studies have associated disease severity with HLA-DR2 and polymorphisms in metabolism and immune response genes. While a number of animal models have been used to investigate the underlying immune mechanisms in TOS, only a few studies in rodents have demonstrated the classical symptoms of TOS. Biotransformation and oxidation of the parent compound(s) to reactive intermediates prior to induction of autoreactive pathways appears to be an important component of the disease process. These reactive intermediates could haptenate self-proteins and activate autoreactive T-cells, disrupt signal transduction, or induce apoptosis and necrosis to release abnormal forms of self-antigens. Although the TOS epidemic was limited to a discrete period of time, the origin of the contamination determined, and the spread of the disease halted by government intervention, the underlying immune mechanisms have yet to be elucidated. JF - Journal of Immunotoxicology AU - Patterson, R AU - Germolec, D AD - Laboratory of Molecular Toxicology, Environmental Toxicology Program, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, North Carolina, USA Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 51 EP - 58 VL - 2 IS - 1 SN - 1547-691X, 1547-691X KW - Immunology Abstracts; Toxicology Abstracts KW - Histocompatibility antigen HLA KW - Apoptosis KW - Contamination KW - Gene polymorphism KW - Autoimmune diseases KW - Animal models KW - biotransformation KW - Oil KW - Necrosis KW - Lymphocytes T KW - Cytokines KW - Epidemics KW - Vasculitis KW - Eosinophilia-myalgia syndrome KW - Toxic oil syndrome KW - Esters KW - Eosinophilia KW - Autoantigens KW - Reviews KW - Oxidation KW - Fasciitis KW - Immune response KW - Contaminants KW - Metabolism KW - Signal transduction KW - X 24240:Miscellaneous KW - X 24250:Reviews KW - F 06930:Autoimmunity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17436098?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunotoxicology&rft.atitle=Review+Article+Toxic+Oil+Syndrome%3A+Review+of+Immune+Aspects+of+the+Disease&rft.au=Patterson%2C+R%3BGermolec%2C+D&rft.aulast=Patterson&rft.aufirst=R&rft.date=2005-03-01&rft.volume=2&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunotoxicology&rft.issn=1547691X&rft_id=info:doi/10.1080%2F15476910590960143 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Histocompatibility antigen HLA; Apoptosis; Contamination; Gene polymorphism; Autoimmune diseases; biotransformation; Animal models; Oil; Necrosis; Lymphocytes T; Cytokines; Vasculitis; Epidemics; Eosinophilia-myalgia syndrome; Toxic oil syndrome; Esters; Eosinophilia; Autoantigens; Reviews; Oxidation; Immune response; Fasciitis; Contaminants; Metabolism; Signal transduction DO - http://dx.doi.org/10.1080/15476910590960143 ER - TY - JOUR T1 - Executive Function Correlates with Walking Speed in Older Persons: The InCHIANTI Study AN - 17346681; 6238259 AB - Objectives: To study the association between performance on psychological tests of executive function and performance on lower extremity tasks with different attentional demands in a large sample of nondemented, older adults. Design: Cross-sectional study. Setting: Community-based. Participants: Nine hundred twenty-six persons aged 65 and older, without dementia, stroke, parkinsonism, visual impairment, or current treatment with neuroleptics, enrolled in a large epidemiological study. Measurements: Trail Making Test (TMT) parts A and B and two performance-based measures of lower extremity function that require different executive-attentional-demanding skills: walking speed on a 4-m course at usual pace and walking speed on a 7-m obstacle course at fast pace. A difference score (Delta TMT), obtained by subtracting time to perform part A from time to perform part B of the TMT, was used as an indicator of executive function. Based on Delta TMT, subjects were divided into poor performance, intermediate performance, and good performance. Results: After adjustment, no association between Delta TMT and 4-m course usual-pace walking speed was found. Participants with poor Delta TMT and with intermediate Delta TMT performance were more likely to be in the lowest tertile for 7-m obstacle course walking speed. Conclusion: In nondemented older persons, executive function is independently associated with tasks of lower extremity function that require high attentional demand. JF - Journal of the American Geriatrics Society AU - Ble, Alesandro AU - Volpato, Stefano AU - Zuliani, Giovanni AU - Guralnik, Jack M AU - Bandinelli, Stefania AU - Lauretani, Fulvio AU - Bartali, Benedetta AU - Maraldi, Cinzia AU - Fellin, Renato AU - Ferrucci, Luigi AD - Luigi Ferrucci, MD, PhD, National Institutes of Heath, National Institute on Aging, Harbor Hospital, 3001 S. Hanover Street, Baltimore, MD 21225, ferruccilu@grc.nia.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 410 EP - 415 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 53 IS - 3 SN - 0002-8614, 0002-8614 KW - Physical Education Index KW - PE 100:Kinesiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17346681?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Geriatrics+Society&rft.atitle=Executive+Function+Correlates+with+Walking+Speed+in+Older+Persons%3A+The+InCHIANTI+Study&rft.au=Ble%2C+Alesandro%3BVolpato%2C+Stefano%3BZuliani%2C+Giovanni%3BGuralnik%2C+Jack+M%3BBandinelli%2C+Stefania%3BLauretani%2C+Fulvio%3BBartali%2C+Benedetta%3BMaraldi%2C+Cinzia%3BFellin%2C+Renato%3BFerrucci%2C+Luigi&rft.aulast=Ble&rft.aufirst=Alesandro&rft.date=2005-03-01&rft.volume=53&rft.issue=3&rft.spage=410&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Geriatrics+Society&rft.issn=00028614&rft_id=info:doi/10.1111%2Fj.1532-5415.2005.53157.x LA - English DB - Physical Education Index N1 - Date revised - 2006-12-01 N1 - SuppNotes - Figures, 1; tables, 2; references, 30. N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1111/j.1532-5415.2005.53157.x ER - TY - JOUR T1 - Global Gene Expression Associated with Hepatocarcinogenesis in Adult Male Mice Induced by in Utero Arsenic Exposure AN - 17230733; 6963734 AB - Our previous work has shown that exposure to inorganic arsenic in utero produces hepatocellular carcinoma (HCC) in adult male mice. To explore further the molecular mechanisms of transplacental arsenic hepatocarcinogenesis, we conducted a second arsenic transplacental carcinogenesis study and used a genomewide microarray to profile arsenic-induced aberrant gene expression more extensively. Briefly, pregnant C3H mice were given drinking water containing 85 ppm arsenic as sodium arsenite or unaltered water from days 8 to 18 of gestation. The incidence of HCC in adult male offspring was increased 4-fold and tumor multiplicity 3-fold after transplacental arsenic exposure. Samples of normal liver and liver tumors were taken at autopsy for genomic analysis. Arsenic exposure in utero resulted in significant alterations (p 90% concordance. Arsenic-altered gene expression included activation of oncogenes and HCC biomarkers, and increased expression of cell proliferation-related genes, stress proteins, and insulin-like growth factors and genes involved in cell-cell communications. Liver feminization was evidenced by increased expression of estrogen-linked genes and altered expression of genes that encode gender-related metabolic enzymes. These novel findings are in agreement with the biology and histology of arsenic-induced HCC, thereby indicating that multiple genetic events are associated with transplacental arsenic hepatocarcinogenesis. JF - Environmental Health Perspectives AU - Liu, Jie AU - Xie, Yaxiong AU - Ducharme, DMK AU - Shen, Jun AU - Diwan, BA AU - Merrick, BA AU - Grissom, S F AU - Tucker, C J AU - Paules, R S AU - Tennant, R AU - Waalkes, M P AD - Inorganic Carcinogenesis Section, NCI at NIEHS, Mail Drop F0-09, Research Triangle Park, NC 27709, USA, waalkes@niehs.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 404 EP - 411 VL - 114 IS - 3 SN - 0091-6765, 0091-6765 KW - Genetics Abstracts; Toxicology Abstracts KW - Autopsy KW - Molecular modelling KW - DNA microarrays KW - Gene expression KW - Myc protein KW - Oncogenes KW - Gestation KW - Polymerase chain reaction KW - Intercellular signalling KW - Hepatocellular carcinoma KW - Western blotting KW - Arsenic KW - Sodium arsenite KW - stress proteins KW - Enzymes KW - Tumors KW - Intrauterine exposure KW - biomarkers KW - Transplacental carcinogenesis KW - Pregnancy KW - Insulin-like growth factors KW - Genomic analysis KW - Progeny KW - Drinking water KW - X 24165:Biochemistry KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17230733?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Global+Gene+Expression+Associated+with+Hepatocarcinogenesis+in+Adult+Male+Mice+Induced+by+in+Utero+Arsenic+Exposure&rft.au=Liu%2C+Jie%3BXie%2C+Yaxiong%3BDucharme%2C+DMK%3BShen%2C+Jun%3BDiwan%2C+BA%3BMerrick%2C+BA%3BGrissom%2C+S+F%3BTucker%2C+C+J%3BPaules%2C+R+S%3BTennant%2C+R%3BWaalkes%2C+M+P&rft.aulast=Liu&rft.aufirst=Jie&rft.date=2005-03-01&rft.volume=114&rft.issue=3&rft.spage=404&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.8534 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Molecular modelling; Autopsy; Western blotting; Arsenic; Sodium arsenite; stress proteins; Enzymes; Intrauterine exposure; Tumors; biomarkers; DNA microarrays; Transplacental carcinogenesis; Pregnancy; Myc protein; Gene expression; Oncogenes; Genomic analysis; Gestation; Insulin-like growth factors; Polymerase chain reaction; Progeny; Drinking water; Intercellular signalling; Hepatocellular carcinoma DO - http://dx.doi.org/10.1289/ehp.8534 ER - TY - JOUR T1 - Exercise treadmill testing using a modified exercise protocol in women with suspected myocardial ischemia: Findings from the National Heart, Lung and Blood Institute-sponsored Women's Ischemia Syndrome Evaluation (WISE) AN - 1504432186; 15864243 AB - Background Exercise testing, a major diagnostic modality in individuals with suspected coronary artery disease (CAD), has in general demonstrated less overall diagnostic accuracy in women compared to men. As part of the WISE, a modified protocol was examined with the intention of improving reliability of exercise testing. Methods Criteria for entry in the WISE study include clinically indicated coronary angiography. Exercise testing was performed using a protocol modified to be more appropriate for women. The study population consisted of 96 women, mean age of 55.8 y (range 34-77), who completed exercise treadmill test (ETT). Most (78%) were postmenopausal; 96% had ?2 risk factors for CAD. Results By core laboratory angiography, 29/96 women had stenosis ?50% in at least one coronary artery. Of these 29 women, 9 had abnormal ETT, yielding overall sensitivity of 31%. The remaining 20 women had normal (12/29, 41%) or nondiagnostic (8/29, 28%) studies. Among the 67 women with minimal or no coronary stenosis, 35 had no ischemic ST-segment changes during ETT, yielding overall specificity of 52%. Analysis with exclusion of women with nondiagnostic studies yielded sensitivity and specificity of 43% and 66%, respectively. The presence of coronary artery stenosis and inability to perform ETT, but not results of testing, predicted the outcomes of myocardial infarction, heart failure, and death. Conclusions Exercise treadmill test appears to be of limited diagnostic value in women with suspected myocardial ischemia referred for coronary angiography. Sensitivity and specificity remain poor even with modified exercise protocol and core laboratory angiographic analysis. These findings merit consideration in view of current guidelines that recommend exercise testing in women with suspected CAD. (Am Heart J 2005;149:1-7.) JF - The American Heart Journal AU - Lewis, Jannet F AU - McGorray, Susan AU - Lin, Lang AU - Pepine, Carl J AU - Chaitman, Bernard AU - Doyle, Mark AU - Edmundowicz, Daniel AU - Sharaf, Barry L AU - Merz, C Noel Bairey Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 527 EP - 33 CY - Philadelphia PB - Elsevier Limited VL - 149 IS - 3 SN - 00028703 KW - Medical Sciences--Cardiovascular Diseases KW - Exercise KW - Heart attacks KW - Coronary vessels KW - Colleges & universities KW - Statistical methods KW - Cardiovascular disease KW - Ischemia KW - Medical imaging KW - Blood pressure KW - Heart rate KW - Patients KW - Heart failure KW - Diabetes KW - Fitness equipment KW - Myocardial Ischemia -- etiology KW - Humans KW - Electrocardiography KW - Coronary Stenosis -- complications KW - Coronary Angiography KW - Adult KW - Aged KW - Middle Aged KW - Follow-Up Studies KW - Sensitivity & Specificity KW - Female KW - Clinical Protocols KW - Exercise Test -- methods KW - Myocardial Ischemia -- diagnosis KW - Women's Health KW - Coronary Stenosis -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1504432186?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+Heart+Journal&rft.atitle=Exercise+treadmill+testing+using+a+modified+exercise+protocol+in+women+with+suspected+myocardial+ischemia%3A+Findings+from+the+National+Heart%2C+Lung+and+Blood+Institute-sponsored+Women%27s+Ischemia+Syndrome+Evaluation+%28WISE%29&rft.au=Lewis%2C+Jannet+F%3BMcGorray%2C+Susan%3BLin%2C+Lang%3BPepine%2C+Carl+J%3BChaitman%2C+Bernard%3BDoyle%2C+Mark%3BEdmundowicz%2C+Daniel%3BSharaf%2C+Barry+L%3BMerz%2C+C+Noel+Bairey&rft.aulast=Lewis&rft.aufirst=Jannet&rft.date=2005-03-01&rft.volume=149&rft.issue=3&rft.spage=527&rft.isbn=&rft.btitle=&rft.title=The+American+Heart+Journal&rft.issn=00028703&rft_id=info:doi/10.1016%2Fj.ahj.2004.03.068 LA - English DB - ProQuest Central N1 - Copyright - Copyright Elsevier Limited Mar 2005 N1 - Last updated - 2014-04-30 N1 - CODEN - AHJOA2 DO - http://dx.doi.org/10.1016/j.ahj.2004.03.068 ER - TY - JOUR T1 - Mechanisms of resistance to anticancer drugs: the role of the polymorphic ABC transporters ABCB1 and ABCG2 AN - 1020847002; 16781670 AB - ATP-binding cassette (ABC) genes play a role in the resistance of malignant cells to anticancer agents. The ABC gene products, including ABCB1 (P-glycoprotein) and ABCG2 (breast cancer-resistance protein [BCRP], mitoxantrone-resistance protein [MXR], or ABC transporter in placenta [ABCP]), are also known to influence oral absorption and disposition of a wide variety of drugs. As a result, the expression levels of these proteins in humans have important consequences for an individual's susceptibility to certain drug-induced side effects, interactions, and treatment efficacy. Naturally occurring variants in ABC transporter genes have been identified that might affect the function and expression of the protein. This review focuses on recent advances in the pharmacogenetics of the ABC transporters ABCB1 and ABCG2, and discusses potential implications of genetic variants for the chemotherapeutic treatment of cancer. JF - Pharmacogenomics AU - Lepper, Erin R AU - Nooter, Kees AU - Verweij, Jaap AU - Acharya, Milin R AU - Figg, William D AU - Sparreboom, Alex AD - National Cancer Institute, Clinical Pharmacology Research Core, Building 10, Room 5A01, 9000 Rockville Pike, Bethesda, MD 20892, USATel.: +1 (301) 402 9498; Fax: +1 (301) 402 8606; E-mail:, sparreba@mail.nih.gov Y1 - 2005/03// PY - 2005 DA - Mar 2005 SP - 115 EP - 138 PB - Future Science Group (FSG), Unitec House, 2 Albert Place London N3 1QB United Kingdom VL - 6 IS - 2 SN - 1462-2416, 1462-2416 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - ABC transporter KW - G 07720:Immunogenetics KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1020847002?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacogenomics&rft.atitle=Mechanisms+of+resistance+to+anticancer+drugs%3A+the+role+of+the+polymorphic+ABC+transporters+ABCB1+and+ABCG2&rft.au=Lepper%2C+Erin+R%3BNooter%2C+Kees%3BVerweij%2C+Jaap%3BAcharya%2C+Milin+R%3BFigg%2C+William+D%3BSparreboom%2C+Alex&rft.aulast=Lepper&rft.aufirst=Erin&rft.date=2005-03-01&rft.volume=6&rft.issue=2&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Pharmacogenomics&rft.issn=14622416&rft_id=info:doi/10.1517%2F14622416.6.2.115 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2012-06-01 N1 - Number of references - 1 N1 - Last updated - 2014-03-10 N1 - SubjectsTermNotLitGenreText - ABC transporter DO - http://dx.doi.org/10.1517/14622416.6.2.115 ER - TY - JOUR T1 - Mitochondria, oxidants, and aging. AN - 67470702; 15734681 AB - The free radical theory of aging postulates that the production of intracellular reactive oxygen species is the major determinant of life span. Numerous cell culture, invertebrate, and mammalian models exist that lend support to this half-century-old hypothesis. Here we review the evidence that both supports and conflicts with the free radical theory and examine the growing link between mitochondrial metabolism, oxidant formation, and the biology of aging. JF - Cell AU - Balaban, Robert S AU - Nemoto, Shino AU - Finkel, Toren AD - Laboratory of Cardiac Energetics, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/02/25/ PY - 2005 DA - 2005 Feb 25 SP - 483 EP - 495 VL - 120 IS - 4 SN - 0092-8674, 0092-8674 KW - Free Radicals KW - 0 KW - Oxidants KW - Reactive Oxygen Species KW - Index Medicus KW - Yeasts KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Mutation -- physiology KW - Mutation -- genetics KW - Nutritional Physiological Phenomena -- physiology KW - Aging -- physiology KW - Mitochondria -- metabolism KW - Oxidants -- metabolism KW - Free Radicals -- metabolism KW - Mitochondria -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67470702?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell&rft.atitle=Mitochondria%2C+oxidants%2C+and+aging.&rft.au=Balaban%2C+Robert+S%3BNemoto%2C+Shino%3BFinkel%2C+Toren&rft.aulast=Balaban&rft.aufirst=Robert&rft.date=2005-02-25&rft.volume=120&rft.issue=4&rft.spage=483&rft.isbn=&rft.btitle=&rft.title=Cell&rft.issn=00928674&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-14 N1 - Date created - 2005-02-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Chromatin-dependent E1A activity modulates NF-kappaB RelA-mediated repression of glucocorticoid receptor-dependent transcription. AN - 67446227; 15556937 AB - The role of chromatin-dependent regulatory mechanisms in the repression of glucocorticoid-dependent transcription from the murine mammary tumor virus (MMTV) promoter by p65 and E1A was investigated by using chromatin and transiently transfected reporters. The p65 RelA subunit of NF-kappaB represses MMTV expression on either transient or integrated reporters. In contrast, the viral oncoprotein E1A represses a transient but not an integrated MMTV. E1A repression is attenuated by chromatin, suggesting p65 but not E1A manipulates chromatin appropriately to inhibit the GR. Coexpression of p65 and E1A additively represses the transient MMTV but restores the transcriptional activation of the chromatin MMTV in response to glucocorticoids. This indicates that E1A has a dominant chromatin-dependent activity that attenuates repression by p65. E1A, p65, and GR bind the MMTV promoter, and chromatin remodeling enhances binding on both repressed and activated promoters. In addition, p65 requires Brg for repression of the integrated MMTV. This suggests that neither p65 repression nor E1A attenuation of repression results from an inhibition of remodeling that prevents transcription factor binding. Furthermore, p300/CBP is also required for both repression and attenuation by p65 and E1A. E1A and p65 mutants that do not bind p300/CBP are inactive, indicative of a requirement for p300/CBP-dependent complex formation for both repression and attenuation with chromatin. These data suggest that both the p65-dependent repression and the E1A-mediated attenuation of repression require the Brg1-dependent chromatin remodeling function and p300/CBP-dependent complex formation at a promoter assembled within chromatin. JF - The Journal of biological chemistry AU - Burkhart, Barbara A AU - Hebbar, Pratibha B AU - Trotter, Kevin W AU - Archer, Trevor K AD - Laboratory of Molecular Carcinogenesis, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/02/25/ PY - 2005 DA - 2005 Feb 25 SP - 6349 EP - 6358 VL - 280 IS - 8 SN - 0021-9258, 0021-9258 KW - Chromatin KW - 0 KW - FGF3 protein, human KW - Fibroblast Growth Factor 3 KW - NF-kappa B KW - Nuclear Proteins KW - Receptors, Glucocorticoid KW - Repressor Proteins KW - Trans-Activators KW - Transcription Factor RelA KW - Transcription Factors KW - Fibroblast Growth Factors KW - 62031-54-3 KW - SMARCA4 protein, human KW - EC 3.6.1.- KW - DNA Helicases KW - EC 3.6.4.- KW - Index Medicus KW - Transcription Factors -- physiology KW - Promoter Regions, Genetic KW - Humans KW - Chromatin Assembly and Disassembly KW - Fibroblast Growth Factors -- genetics KW - Cell Line KW - Repressor Proteins -- physiology KW - Chromatin -- physiology KW - Transcription, Genetic KW - Gene Expression Regulation KW - Trans-Activators -- physiology KW - NF-kappa B -- physiology KW - Receptors, Glucocorticoid -- physiology KW - Nuclear Proteins -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67446227?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Chromatin-dependent+E1A+activity+modulates+NF-kappaB+RelA-mediated+repression+of+glucocorticoid+receptor-dependent+transcription.&rft.au=Burkhart%2C+Barbara+A%3BHebbar%2C+Pratibha+B%3BTrotter%2C+Kevin+W%3BArcher%2C+Trevor+K&rft.aulast=Burkhart&rft.aufirst=Barbara&rft.date=2005-02-25&rft.volume=280&rft.issue=8&rft.spage=6349&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-08 N1 - Date created - 2005-02-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Novel Anticholinesterases Based on the Molecular Skeletons of Furobenzofuran and Methanobenzodioxepinev AN - 19384201; 7149661 AB - Reductive cyclization of 5-hydroxy-3-methyl-3-methoxycarbonylmethylenebenzofuran-2(3H)-one (4) gave 5-hydroxy-3a-methyl-2,3,3a,8a-tatrahydrofiiro[2,3-b]benzofuran (5) and the rearrangement product 7-hydroxy-4,5-dihydro-2,5-methano-1,3-benzodioxepine (6). Reaction of compounds 5 and 6 with different isocyanates provided two series novel carbamates (7-12) whose structures were confirmed by X-ray crystallography. These were assessed for anticholinesterase action against freshly prepared human enzyme and proved to be potent inhibitors of either acetyl-(AChE) or butyrylcholinesterase (BChE) with specific compounds exhibiting remarkable selectivity. Because the two series of carbamates (7-12) differ in their phenolic moieties, their respective potency and selectivity for AChE versus BChE was governed by their N-substituted groups. This same characteristic was also present in a series of physovenine analogues (1,13,15,17) and physostigmine analogues (2, 14, 16, 18). These structure-activity relations proved valuable in elucidating the mechanisms underpinning the interaction between carbamate-based cholinesterase inhibitors and their enzyme target. In addition, because physostigmine analogues have demonstrated activity in lowering the Alzheimer's disease protein, amyloid precursor protein (APP), examples of the two new series of carbamates were characterized in culture assays of quantifying cell viability and synthesis of APP. JF - Journal of Medicinal Chemistry AU - Luo, W AU - Yu, Q-S AU - Zhan, M AU - Parrish, D AU - Deschamps, J R AU - Kulkarni, S S AU - Holloway, H W AU - Alley, G M AU - Lahiri, D K AU - Brossi, A AU - Greig, N H AD - Drug Design & Development Section, Laboratory of Neurosciences, and Bioinformatics Unit, Research Resources Branch, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA Y1 - 2005/02/24/ PY - 2005 DA - 2005 Feb 24 SP - 986 EP - 994 VL - 48 IS - 4 SN - 0022-2623, 0022-2623 KW - Biotechnology and Bioengineering Abstracts KW - X-ray crystallography KW - Neurodegenerative diseases KW - Alzheimer's disease KW - phenolic compounds KW - Enzymes KW - Cell culture KW - Physostigmine KW - Cholinesterase KW - isocyanates KW - Amyloid precursor protein KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19384201?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Novel+Anticholinesterases+Based+on+the+Molecular+Skeletons+of+Furobenzofuran+and+Methanobenzodioxepinev&rft.au=Luo%2C+W%3BYu%2C+Q-S%3BZhan%2C+M%3BParrish%2C+D%3BDeschamps%2C+J+R%3BKulkarni%2C+S+S%3BHolloway%2C+H+W%3BAlley%2C+G+M%3BLahiri%2C+D+K%3BBrossi%2C+A%3BGreig%2C+N+H&rft.aulast=Luo&rft.aufirst=W&rft.date=2005-02-24&rft.volume=48&rft.issue=4&rft.spage=986&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm049309%2B LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Alzheimer's disease; Amyloid precursor protein; Physostigmine; Enzymes; X-ray crystallography; Cholinesterase; isocyanates; Cell culture; Neurodegenerative diseases; phenolic compounds DO - http://dx.doi.org/10.1021/jm049309+ ER - TY - JOUR T1 - Identification of a dopamine transporter ligand that blocks the stimulant effects of cocaine. AN - 67454773; 15728828 AB - There is a large unmet medical need for cocaine addiction treatments. Studies have indicated that the dopamine transporter (DAT) is the primary biological target of cocaine, and most drugs that have DAT affinity have behavioral effects like those of cocaine. However, analogs of benztropine have high DAT affinity and behavioral effects that show varying degrees of similarity to cocaine. We now report the discovery that a benztropine analog, JHW007, with high affinity for the DAT does not have cocaine-like behavioral effects and antagonizes the effects of cocaine. JHW007 occupied the DAT in vivo more slowly than did cocaine and had not reached an apparent plateau up to 270 min after injection. The in vivo binding of cocaine to the DAT suggested rate of DAT occupancy as an important contributor to its behavioral effects, and the slow association with the DAT may provide an explanation for JHW007 being relatively devoid of cocaine-like behavioral effects. The antagonism of cocaine suggests that DAT ligands with reduced cocaine-like activity can function as cocaine antagonists and suggests JHW007 as a lead for discovery of cocaine-abuse pharmacotherapeutics. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Desai, Rajeev I AU - Kopajtic, Theresa A AU - Koffarnus, Mikhail AU - Newman, Amy Hauck AU - Katz, Jonathan L AD - Psychobiology, Medications Discovery Research Branch, National Institute on Drug Abuse, Intramural Research Program, Department of Health and Human Services, National Institutes of Health, Baltimore, Maryland 21224, USA. Y1 - 2005/02/23/ PY - 2005 DA - 2005 Feb 23 SP - 1889 EP - 1893 VL - 25 IS - 8 KW - Central Nervous System Stimulants KW - 0 KW - Dopamine Antagonists KW - Dopamine Plasma Membrane Transport Proteins KW - Ligands KW - Membrane Glycoproteins KW - Membrane Transport Proteins KW - N-(n-butyl)-(bis-fluorophenyl)methoxytropane KW - N-allyl-(bisfluorophenyl)methoxytropane KW - Nerve Tissue Proteins KW - Slc6a3 protein, mouse KW - Slc6a3 protein, rat KW - RTI 121 KW - 146145-21-3 KW - Benztropine KW - 1NHL2J4X8K KW - Cocaine KW - I5Y540LHVR KW - Index Medicus KW - Animals KW - Dose-Response Relationship, Drug KW - Corpus Striatum -- metabolism KW - Dopamine Antagonists -- pharmacology KW - Mice KW - Cerebellum -- metabolism KW - Rats KW - Cerebellum -- drug effects KW - Corpus Striatum -- drug effects KW - Motor Activity -- drug effects KW - Inhibitory Concentration 50 KW - Male KW - Nerve Tissue Proteins -- drug effects KW - Membrane Glycoproteins -- drug effects KW - Cocaine -- analogs & derivatives KW - Benztropine -- analogs & derivatives KW - Membrane Transport Proteins -- drug effects KW - Central Nervous System Stimulants -- antagonists & inhibitors KW - Benztropine -- pharmacology KW - Cocaine -- pharmacology KW - Cocaine -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67454773?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Identification+of+a+dopamine+transporter+ligand+that+blocks+the+stimulant+effects+of+cocaine.&rft.au=Desai%2C+Rajeev+I%3BKopajtic%2C+Theresa+A%3BKoffarnus%2C+Mikhail%3BNewman%2C+Amy+Hauck%3BKatz%2C+Jonathan+L&rft.aulast=Desai&rft.aufirst=Rajeev&rft.date=2005-02-23&rft.volume=25&rft.issue=8&rft.spage=1889&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-26 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Solutes probe hydration in specific association of cyclodextrin and adamantane. AN - 67436324; 15713096 AB - Using microcalorimetry, we follow changes in the association free energy of beta-cyclodextrin (CD) with the hydrophobic part of adamantane carboxylate (AD) due to added salt or polar (net-neutral) solutes that are excluded from the molecular interacting surfaces. Changes in binding constants with solution osmotic pressure (water activity) translate into changes in the preferential hydration upon complex formation. We find that these changes correspond to a release of 15-25 solute-excluding waters upon CD/AD association. Reflecting the preferential interaction of solute with reactants versus products, we find that changes in hydration depend on the type of solute used. All solutes used here result in a large change in the enthalpy of the CD-AD binding reaction. In one class of solutes, the corresponding entropy change is much smaller, while in the other class, the entropy change almost fully compensates the solute-specific enthalpy. For many of the solutes, the number of waters released correlates well with their effect on air-water surface tensions. We corroborate these results using vapor pressure osmometry to probe individually the hydration of reactants and products of association, and we discuss the possible interactions and forces between cosolute and hydrophobic surfaces responsible for different kinds of solute exclusion. JF - Journal of the American Chemical Society AU - Harries, Daniel AU - Rau, Donald C AU - Parsegian, V Adrian AD - Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-0924, USA. harries@helix.nih.gov Y1 - 2005/02/23/ PY - 2005 DA - 2005 Feb 23 SP - 2184 EP - 2190 VL - 127 IS - 7 SN - 0002-7863, 0002-7863 KW - beta-Cyclodextrins KW - 0 KW - Water KW - 059QF0KO0R KW - adamantanecarboxylic acid KW - 828-51-3 KW - betadex KW - JV039JZZ3A KW - Adamantane KW - PJY633525U KW - Index Medicus KW - Calorimetry -- methods KW - Osmotic Pressure KW - Thermodynamics KW - Water -- chemistry KW - Adamantane -- chemistry KW - beta-Cyclodextrins -- chemistry KW - Adamantane -- analogs & derivatives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67436324?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Chemical+Society&rft.atitle=Solutes+probe+hydration+in+specific+association+of+cyclodextrin+and+adamantane.&rft.au=Harries%2C+Daniel%3BRau%2C+Donald+C%3BParsegian%2C+V+Adrian&rft.aulast=Harries&rft.aufirst=Daniel&rft.date=2005-02-23&rft.volume=127&rft.issue=7&rft.spage=2184&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Chemical+Society&rft.issn=00027863&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-18 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cardiovascular risk factors and migraine: the GEM population-based study. AN - 67459160; 15728281 AB - Migraine, particularly with aura, is a risk factor for early-onset ischemic stroke. The underlying mechanisms are unknown, but may in part be due to migraineurs having an increased risk profile for cardiovascular disease. In this study, the authors compare the cardiovascular risk profile of adult migraineurs to that of nonmigraineurs. Participants (n = 5,755, 48% men, age 20 to 65 years) are from the Genetic Epidemiology of Migraine (GEM) study, a population-based study in the Netherlands. A total of 620 current migraineurs were identified: 31% with aura (MA), 64% without aura (MO), and 5% unclassified. Controls were 5,135 individuals without lifetime migraine. Measured cardiovascular risk factors included blood pressure (BP), serum total and high-density lipoprotein cholesterol (TC, HDL), smoking, oral contraceptive use, and the Framingham risk score for myocardial infarction or coronary heart disease (CHD) death. Compared to controls, migraineurs were more likely to smoke (OR = 1.43 [1.1 to 1.8]), less likely to consume alcohol (OR = 0.58 [0.5 to 0.7]), and more likely to report a parental history of early myocardial infarction. Migraineurs with aura were more likely to have an unfavorable cholesterol profile (TC > or = 240 mg/dL [OR = 1.43 (0.97 to 2.1)], TC:HDL ratio > 5.0 [OR = 1.64 (1.1 to 2.4)]), have elevated BP (systolic BP > 140 mm Hg or diastolic BP > 90 mm Hg [OR = 1.76 (1.04 to 3.0)]), and report a history of early onset CHD or stroke (OR = 3.96 [1.1 to 14.3]); female migraineurs with aura were more likely to be using oral contraceptives (OR = 2.06 [1.05 to 4.0]). The odds of having an elevated Framingham risk score for CHD were approximately doubled for the migraineurs with aura. Migraineurs, particularly with aura, have a higher cardiovascular risk profile than individuals without migraine. JF - Neurology AU - Scher, A I AU - Terwindt, G M AU - Picavet, H S J AU - Verschuren, W M M AU - Ferrari, M D AU - Launer, L J AD - Laboratory of Epidemiology, Demography, and Biometry, National Institute on Aging, National Institutes of Health, Bethesda, MD, USA. ascher@usuhs.mil Y1 - 2005/02/22/ PY - 2005 DA - 2005 Feb 22 SP - 614 EP - 620 VL - 64 IS - 4 KW - Contraceptives, Oral, Hormonal KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - Netherlands -- epidemiology KW - Pregnancy Complications, Cardiovascular -- epidemiology KW - Myocardial Infarction -- genetics KW - Humans KW - Aged KW - Contraceptives, Oral, Hormonal -- adverse effects KW - Alcohol Drinking -- epidemiology KW - Smoking -- epidemiology KW - Pregnancy KW - Stroke -- epidemiology KW - Migraine without Aura -- epidemiology KW - Cross-Sectional Studies KW - Migraine with Aura -- epidemiology KW - Social Class KW - Risk Factors KW - Hypertension -- epidemiology KW - Adult KW - Middle Aged KW - Female KW - Male KW - Hypercholesterolemia -- epidemiology KW - Migraine Disorders -- epidemiology KW - Cardiovascular Diseases -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67459160?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurology&rft.atitle=Cardiovascular+risk+factors+and+migraine%3A+the+GEM+population-based+study.&rft.au=Scher%2C+A+I%3BTerwindt%2C+G+M%3BPicavet%2C+H+S+J%3BVerschuren%2C+W+M+M%3BFerrari%2C+M+D%3BLauner%2C+L+J&rft.aulast=Scher&rft.aufirst=A&rft.date=2005-02-22&rft.volume=64&rft.issue=4&rft.spage=614&rft.isbn=&rft.btitle=&rft.title=Neurology&rft.issn=1526-632X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-18 N1 - Date created - 2005-02-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Neurology. 2005 Nov 22;65(10):1683; author reply 1683 [16301516] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Agonist-induced conformational changes in thyrotropin-releasing hormone receptor type I: disulfide cross-linking and molecular modeling approaches. AN - 67429357; 15709754 AB - The conformational changes at the cytoplasmic ends of transmembrane helices 5 and 6 (TMH5 and TMH6) of thyrotropin-releasing hormone (TRH) receptor type I (TRH-R1) during activation were analyzed by cysteine-scanning mutagenesis followed by disulfide cross-linking and molecular modeling. Sixteen double cysteine mutants were constructed by substitution of one residue at the cytoplasmic end of TMH5 and the other at that of TMH6. The cross-linking experiments indicate that four mutants, Q263C/G212C, Q263C/Y211C, T265C/G212C, and T265C/Y211C, exhibited disulfide bond formation that was sensitive to TRH occupancy. We refined our previous TRH-R1 models by embedding them into a hydrated explicit lipid bilayer. Molecular dynamics simulations of the models, as well as in silico double cysteine models, generated trajectories that were in agreement with experimental results. Our findings suggest that TRH binding induces a separation of the cytoplasmic ends of TMH5 and TMH6 and a rotation of TMH6. These changes likely increase the surface accessible area at the juxtamembrane region of intracellular loop 3 that could promote interactions between G proteins and key residues within the receptor. JF - Biochemistry AU - Huang, Wei AU - Osman, Roman AU - Gershengorn, Marvin C AD - Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Building 50/4134, Bethesda, Maryland 20892-1818, USA. Y1 - 2005/02/22/ PY - 2005 DA - 2005 Feb 22 SP - 2419 EP - 2431 VL - 44 IS - 7 SN - 0006-2960, 0006-2960 KW - Cross-Linking Reagents KW - 0 KW - Disulfides KW - Phenanthrolines KW - Receptors, Thyrotropin-Releasing Hormone KW - Thyrotropin-Releasing Hormone KW - 5Y5F15120W KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Animals KW - Computer Simulation KW - Cysteine -- genetics KW - Humans KW - Computational Biology -- methods KW - Amino Acid Sequence KW - Mice KW - Cytoplasm -- chemistry KW - Mutagenesis, Site-Directed KW - Cysteine -- chemistry KW - Phenanthrolines -- chemistry KW - Cytoplasm -- genetics KW - Cytoplasm -- metabolism KW - Molecular Sequence Data KW - Protein Structure, Secondary -- genetics KW - Cell Line KW - Protein Conformation KW - Receptors, Thyrotropin-Releasing Hormone -- chemistry KW - Receptors, Thyrotropin-Releasing Hormone -- agonists KW - Disulfides -- chemistry KW - Cross-Linking Reagents -- chemistry KW - Models, Molecular KW - Thyrotropin-Releasing Hormone -- metabolism KW - Receptors, Thyrotropin-Releasing Hormone -- genetics KW - Thyrotropin-Releasing Hormone -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67429357?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Agonist-induced+conformational+changes+in+thyrotropin-releasing+hormone+receptor+type+I%3A+disulfide+cross-linking+and+molecular+modeling+approaches.&rft.au=Huang%2C+Wei%3BOsman%2C+Roman%3BGershengorn%2C+Marvin+C&rft.aulast=Huang&rft.aufirst=Wei&rft.date=2005-02-22&rft.volume=44&rft.issue=7&rft.spage=2419&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-25 N1 - Date created - 2005-02-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Strategic interactions in multi-institutional epidemics of antibiotic resistance AN - 19818902; 6175319 AB - The increasing frequency of antibiotic resistance in hospital-acquired infections is a major public health concern that has both biological and economic causes. Here we develop conceptual mathematical models that couple the economic incentives and population biology of hospital infection control (HIC). We show that the optimal investment by a hospital for HIC changes with the proportion of patients already colonized with antibiotic-resistant bacteria (ARB) at the time of admission. As that proportion increases, the optimal behavior of a hospital is to increase spending to control ARB with low transmissibility and decrease spending on those with high transmissibility. In some cases, the global optimum investment in HIC can shift discontinuously from one that contains transmission to a do-nothing policy once the proportion already colonized at the time of admission becomes too great. We also show that investments in HIC are determined by a strategic game when several hospitals share patients. Hospitals acting selfishly and rationally will free-ride on the investments of other hospitals, and the level of free-riding should increase with the number of other hospitals in the area. Thus, in areas with many hospitals, the rational strategy for each hospital is to spend less than in areas with few hospitals. Thus, we predict that transmission rates and the prevalence of ARB should be higher in urban hospitals, for instance, compared with rural hospitals. We conclude that regional coordination and planning for HIC is an essential element of public health planning for hospital-acquired infections. JF - Proceedings of the National Academy of Sciences, USA AU - Smith, David L AU - Levin, Simon A AU - Laxminarayan, Ramanan AD - Fogarty International Center, National Institutes of Health, Bethesda, MD Y1 - 2005/02/22/ PY - 2005 DA - 2005 Feb 22 SP - 3153 EP - 3158 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 8 SN - 0027-8424, 0027-8424 KW - Microbiology Abstracts B: Bacteriology KW - Mathematical models KW - Epidemics KW - Economics KW - Infection KW - Antibiotic resistance KW - Hospitals KW - Public health KW - Disease transmission KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19818902?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Strategic+interactions+in+multi-institutional+epidemics+of+antibiotic+resistance&rft.au=Smith%2C+David+L%3BLevin%2C+Simon+A%3BLaxminarayan%2C+Ramanan&rft.aulast=Smith&rft.aufirst=David&rft.date=2005-02-22&rft.volume=102&rft.issue=8&rft.spage=3153&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Epidemics; Mathematical models; Economics; Infection; Antibiotic resistance; Disease transmission; Public health; Hospitals ER - TY - JOUR T1 - Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization AN - 17525180; 6175269 AB - Professional antigen-presenting dendritic cells (DCs) are critical in regulating T cell immune responses at both systemic and mucosal sites. Many Lactobacillus species are normal members of the human gut microflora and most are regarded as safe when administered as probiotics. Because DCs can naturally or therapeutically encounter lactobacilli, we investigated the effects of several well defined strains, representing three species of Lactobacillus on human myeloid DCs (MDCs) and found that they modulated the phenotype and functions of human MDCs. Lactobacillus-exposed MDCs up-regulated HLA-DR, CD83, CD40, CD80, and CD86 and secreted high levels of IL-12 and IL-18, but not IL-10. IL-12 was sustained in MDCs exposed to all three Lactobacillus species in the presence of LPS from Escherichia coli, whereas LPS-induced IL-10 was greatly inhibited. MDCs activated with lactobacilli clearly skewed CD4 super(+) and CD8 super(+) T cells to T helper 1 and Tc1 polarization, as evidenced by secretion of IFN- gamma, but not IL-4 or IL-13. These results emphasize a potentially important role for lactobacilli in modulating immunological functions of DCs and suggest that certain strains could be particularly advantageous as vaccine adjuvants, by promoting DCs to regulate T cell responses toward T helper 1 and Tc1 pathways. JF - Proceedings of the National Academy of Sciences, USA AU - Mohamadzadeh, Mansour AU - Olson, Scott AU - Kalina, Warren V AU - Ruthel, Gordon AU - Demmin, Gretchen L AU - Warfield, Kelly L AU - Bavari, Sina AU - Klaenhammer, Todd R AD - National Cancer Institute, Frederick, MD Y1 - 2005/02/22/ PY - 2005 DA - 2005 Feb 22 SP - 2880 EP - 2885 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 102 IS - 8 SN - 0027-8424, 0027-8424 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - Histocompatibility antigen HLA KW - CD83 antigen KW - CD86 antigen KW - Adjuvants KW - Interleukin 10 KW - Interleukin 12 KW - Dendritic cells KW - Interleukin 13 KW - CD4 antigen KW - Lactobacillus KW - Interleukin 18 KW - Lymphocytes T KW - Escherichia coli KW - Lipopolysaccharides KW - CD80 antigen KW - CD40 antigen KW - g-Interferon KW - probiotics KW - CD8 antigen KW - Polarization KW - Digestive tract KW - ^g-Interferon KW - Vaccines KW - F 06106:Bacteria KW - J 02833:Immune response and immune mechanisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17525180?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Lactobacilli+activate+human+dendritic+cells+that+skew+T+cells+toward+T+helper+1+polarization&rft.au=Mohamadzadeh%2C+Mansour%3BOlson%2C+Scott%3BKalina%2C+Warren+V%3BRuthel%2C+Gordon%3BDemmin%2C+Gretchen+L%3BWarfield%2C+Kelly+L%3BBavari%2C+Sina%3BKlaenhammer%2C+Todd+R&rft.aulast=Mohamadzadeh&rft.aufirst=Mansour&rft.date=2005-02-22&rft.volume=102&rft.issue=8&rft.spage=2880&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Lactobacillus; Escherichia coli; Dendritic cells; Lymphocytes T; Interleukin 12; Interleukin 10; CD4 antigen; CD80 antigen; g-Interferon; probiotics; Digestive tract; Vaccines; Adjuvants; Polarization; ^g-Interferon; CD86 antigen; CD8 antigen; CD40 antigen; Interleukin 18; CD83 antigen; Histocompatibility antigen HLA; Interleukin 13; Lipopolysaccharides ER - TY - JOUR T1 - Identification of the epitope of a monoclonal antibody to DJ-1. AN - 67373498; 15663963 AB - Mutations in DJ-1 can cause early onset parkinsonism. Various antibodies have been generated to detect this protein, one of which is a commonly used monoclonal antibody (clone 3E8). Since results of in situ examinations of DJ-1 expression with this antibody have differed from analyses with species-specific antibodies (e.g. rat), it would be useful to know the epitope for this antibody. Using GFP-tagged deletion constructs of human DJ-1, we have localized the epitope region for this antibody to within residues 56-78 of human DJ-1. Mapping this region to the published three-dimensional structure of DJ-1 indicates that this is a solvent-accessible surface epitope. Immunonegativity of E64D mutant DJ-1 with the monoclonal antibody suggests that glutamate 64 of human DJ-1 contributes to the epitope recognized by this antibody. Moreover, the loss of immunoreactivity due to such a small substitution demonstrates the remarkable sensitivity of the monoclonal antibody 3E8 to DJ-1. JF - Neuroscience letters AU - Miller, David W AU - Wilson, Carmen R AU - Kaleem, Mona A AU - Blackinton, Jeff AU - Cookson, Mark R AD - Laboratory of Neurogenetics, National Institute on Aging, Bldg 35, Rm 1A-1002, 35 Convent Drive, Bethesda, MD 20892, USA. millerda@mail.nih.gov Y1 - 2005/02/21/ PY - 2005 DA - 2005 Feb 21 SP - 203 EP - 206 VL - 374 IS - 3 SN - 0304-3940, 0304-3940 KW - Antibodies, Monoclonal KW - 0 KW - Epitopes KW - Intracellular Signaling Peptides and Proteins KW - Oncogene Proteins KW - PARK7 protein, human KW - EC 3.1.2.- KW - Protein Deglycase DJ-1 KW - Index Medicus KW - Animals KW - Antigen-Antibody Reactions KW - Protein Structure, Secondary KW - Humans KW - Amino Acid Sequence KW - Blotting, Western -- methods KW - Mice KW - Rats KW - Transfection -- methods KW - Mutagenesis, Site-Directed -- immunology KW - Cell Line KW - Antibody Specificity KW - Oncogene Proteins -- immunology KW - Antibodies, Monoclonal -- chemistry KW - Epitopes -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67373498?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+letters&rft.atitle=Identification+of+the+epitope+of+a+monoclonal+antibody+to+DJ-1.&rft.au=Miller%2C+David+W%3BWilson%2C+Carmen+R%3BKaleem%2C+Mona+A%3BBlackinton%2C+Jeff%3BCookson%2C+Mark+R&rft.aulast=Miller&rft.aufirst=David&rft.date=2005-02-21&rft.volume=374&rft.issue=3&rft.spage=203&rft.isbn=&rft.btitle=&rft.title=Neuroscience+letters&rft.issn=03043940&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-03 N1 - Date created - 2005-01-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Reactions of Benzene Oxide with Thiols Including Glutathione AN - 17832570; 6194511 AB - S-Phenylmercapturic acid is a minor metabolite of benzene used as a biomarker for human benzene exposures. The reaction of intracellular glutathione with benzene oxide-oxepin, the initial metabolite of benzene, is presumed to give 1-(S-glutathionyl)-cyclohexa-3,5-dien-2-ol, which undergoes dehydration to S-phenylglutathione, the precursor of S-phenylmercapturic acid. To validate the proposed route to S-phenylglutathione, reactions of benzene oxide-oxepin with glutathione and other sulfur nucleophiles have been studied. The reaction of benzene oxide with an excess of aqueous sodium sulfide, followed by acetylation, gave bis-(6-trans-5-acetoxycyclohexa-1,3-dienyl)sulfide, the structure of which was proved by X-ray crystallography. Reactions of benzene oxide-oxepin in a 95:5 (v/v) mixture of phosphate buffer in D sub(2)O with (CD sub(3)) sub(2)SO were monitored by super(1)H NMR spectroscopy. In the absence of glutathione, the half-life of benzene oxide-oxepin was ca. 34 min at 25 degree C and pD 7.0. The half-life was not affected in the range of 2-15 mM glutathione in the presence and absence of a commercial sample of human glutathione S-transferase (at pH 7.0, 8.0, 8.5, or 10.0). The adduct 1-(S-glutathionyl)-cyclohexa-3,5-diene-2-ol was identified in these reaction mixtures, especially at higher pH, by mass spectrometry and by its acid-catalyzed decomposition to S-phenylglutathione. Incubation of benzene oxide with N-acetyl-L-cysteine at 37 degree C and pH 10.0 and subsequent mass spectrometric analysis of the mixture showed formation of pre-S-phenylmercapturic acid and the dehydration product, S-phenylmercapturic acid. The data validate the premise that benzene oxide-oxepin can be captured by glutathione to give (1R,2R)- and/or (1S,2S)-1-(S-glutathionyl)-cyclohexa-3,5-dien-2-ol, which dehydrate to S-phenylglutathione. The capture is a relatively inefficient process at pH 7 that is accelerated at higher pH. These studies account for the observation that the metabolism of benzene is dominated by the formation of phenol. The pathway leading to S-phenylmercapturic acid is necessarily minor on account of the low efficiency of benzene oxide capture by glutathione at pH 7 vs spontaneous rearrangement to phenol. JF - Chemical Research in Toxicology AU - Henderson, A P AU - Barnes, M L AU - Bleasdale, C AU - Cameron, R AU - Clegg, W AU - Heath, S L AU - Lindstrom, AB AU - Rappaport, S M AU - Waidyanatha, S AU - Watson, W P AU - Golding, B T AD - School of Natural Sciences-Chemistry, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NEI 7RU, United Kingdom Y1 - 2005/02/21/ PY - 2005 DA - 2005 Feb 21 SP - 265 EP - 270 VL - 18 IS - 2 SN - 0893-228X, 0893-228X KW - Toxicology Abstracts KW - Sulfur KW - Adducts KW - Metabolites KW - Glutathione transferase KW - biomarkers KW - Decomposition KW - Phenols KW - Benzene KW - X-ray crystallography KW - Sodium KW - Sulfide KW - Nucleophiles KW - Acetylation KW - N-Acetyl-L-cysteine KW - Phosphate KW - Magnetic resonance spectroscopy KW - Thiols KW - oxides KW - Metabolism KW - Dehydration KW - X 24222:Analytical procedures UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17832570?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemical+Research+in+Toxicology&rft.atitle=Reactions+of+Benzene+Oxide+with+Thiols+Including+Glutathione&rft.au=Henderson%2C+A+P%3BBarnes%2C+M+L%3BBleasdale%2C+C%3BCameron%2C+R%3BClegg%2C+W%3BHeath%2C+S+L%3BLindstrom%2C+AB%3BRappaport%2C+S+M%3BWaidyanatha%2C+S%3BWatson%2C+W+P%3BGolding%2C+B+T&rft.aulast=Henderson&rft.aufirst=A&rft.date=2005-02-21&rft.volume=18&rft.issue=2&rft.spage=265&rft.isbn=&rft.btitle=&rft.title=Chemical+Research+in+Toxicology&rft.issn=0893228X&rft_id=info:doi/10.1021%2Ftx049781y LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Sulfur; Adducts; Metabolites; Glutathione transferase; Decomposition; biomarkers; Benzene; Phenols; Sodium; X-ray crystallography; Acetylation; Nucleophiles; Sulfide; N-Acetyl-L-cysteine; Phosphate; Magnetic resonance spectroscopy; Thiols; oxides; Metabolism; Dehydration DO - http://dx.doi.org/10.1021/tx049781y ER - TY - JOUR T1 - 3-nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. AN - 67431070; 15710238 AB - 3-nitropropionic acid (3-NPA), a complex II inhibitor of the electron transport chain, causes Huntington disease-like symptoms after administration into animals. However, primary mechanisms of cell death are not clearly understood. This study tested the hypothesis that 3-NPA leads to the generation of reactive oxygen species (ROS), mitochondrial DNA damage, and loss of mitochondrial function. Amplex red and horseradish peroxidase were used to accurately measure the amount of H2O2, and showed that PC12 cells treated with 3-NPA (4 mM) lead to the production of hydrogen peroxide (1 nmol/10(6) cells/h). This amount of 3-NPA also leads to a rapid decline of ATP levels. There was time- and dose-dependent mitochondrial DNA damage following 3-NPA treatment. Overexpression of the proto-oncogene bcl-2 protects cells from apoptosis induced by various stimuli. Overexpression of Bcl-2 leads to almost threefold higher levels of ATP and also decreased the 3-NPA-mediated induction of hydrogen peroxide by over 50%. Bcl-2-overexpressing PC12 cells were also protected from mitochondrial DNA damage. These data show that ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage. JF - Brain research. Molecular brain research AU - Mandavilli, Bhaskar S AU - Boldogh, Istvan AU - Van Houten, Bennett AD - Laboratory of Molecular Genetics, National Institute of Environmental and Health Sciences, National Institutes of Health, 111 Alexander Drive, P.O. Box 12233, Research Triangle Park, NC 27709, USA. Y1 - 2005/02/18/ PY - 2005 DA - 2005 Feb 18 SP - 215 EP - 223 VL - 133 IS - 2 SN - 0169-328X, 0169-328X KW - Convulsants KW - 0 KW - DNA, Mitochondrial KW - Nitro Compounds KW - Propionates KW - Proto-Oncogene Proteins c-bcl-2 KW - RNA, Messenger KW - Hydrogen Peroxide KW - BBX060AN9V KW - 3-nitropropionic acid KW - QY4L0FOX0D KW - Dihydrotachysterol KW - R5LM3H112R KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Dose-Response Relationship, Drug KW - Convulsants -- pharmacology KW - Reverse Transcriptase Polymerase Chain Reaction -- methods KW - Cell Death -- drug effects KW - Flow Cytometry -- methods KW - Rats KW - DNA, Mitochondrial -- drug effects KW - RNA, Messenger -- metabolism KW - Cell Survival -- drug effects KW - Cell Count -- methods KW - DNA, Mitochondrial -- metabolism KW - Gene Expression Regulation -- drug effects KW - Time Factors KW - PC12 Cells KW - Dihydrotachysterol -- metabolism KW - Hydrogen Peroxide -- metabolism KW - Proto-Oncogene Proteins c-bcl-2 -- physiology KW - Proto-Oncogene Proteins c-bcl-2 -- metabolism KW - Propionates -- pharmacology KW - DNA Damage -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67431070?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research.+Molecular+brain+research&rft.atitle=3-nitropropionic+acid-induced+hydrogen+peroxide%2C+mitochondrial+DNA+damage%2C+and+cell+death+are+attenuated+by+Bcl-2+overexpression+in+PC12+cells.&rft.au=Mandavilli%2C+Bhaskar+S%3BBoldogh%2C+Istvan%3BVan+Houten%2C+Bennett&rft.aulast=Mandavilli&rft.aufirst=Bhaskar&rft.date=2005-02-18&rft.volume=133&rft.issue=2&rft.spage=215&rft.isbn=&rft.btitle=&rft.title=Brain+research.+Molecular+brain+research&rft.issn=0169328X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-09 N1 - Date created - 2005-02-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Random mutagenesis of the M3 muscarinic acetylcholine receptor expressed in yeast: identification of second-site mutations that restore function to a coupling-deficient mutant M3 receptor. AN - 67424745; 15572356 AB - The M(3) muscarinic receptor is a prototypical member of the class A family of G protein-coupled receptors (GPCRs). To gain insight into the structural mechanisms governing agonist-mediated M(3) receptor activation, we recently developed a genetically modified yeast strain (Saccharomyces cerevisiae) which allows the efficient screening of large libraries of mutant M(3) receptors to identify mutant receptors with altered/novel functional properties. Class A GPCRs contain a highly conserved Asp residue located in transmembrane domain II (TM II; corresponding to Asp-113 in the rat M(3) muscarinic receptor) which is of fundamental importance for receptor activation. As observed previously with other GPCRs analyzed in mammalian expression systems, the D113N point mutation abolished agonist-induced receptor/G protein coupling in yeast. We then subjected the D113N mutant M(3) receptor to PCR-based random mutagenesis followed by a yeast genetic screen to recover point mutations that can restore G protein coupling to the D113N mutant receptor. A large scale screening effort led to the identification of three such second-site suppressor mutations, R165W, R165M, and Y250D. When expressed in the wild-type receptor background, these three point mutations did not lead to an increase in basal activity and reduced the efficiency of receptor/G protein coupling. Similar results were obtained when the various mutant receptors were expressed and analyzed in transfected mammalian cells (COS-7 cells). Interestingly, like Asp-113, Arg-165 and Tyr-250, which are located at the cytoplasmic ends of TM III and TM V, respectively, are also highly conserved among class A GPCRs. Our data suggest a conformational link between the highly conserved Asp-113, Arg-165, and Tyr-250 residues which is critical for receptor activation. JF - The Journal of biological chemistry AU - Li, Bo AU - Nowak, Nicola M AU - Kim, Soo-Kyung AU - Jacobson, Kenneth A AU - Bagheri, Ali AU - Schmidt, Clarice AU - Wess, Jürgen AD - Department of Molecular Signaling, Laboratory of Bioorganic Chemistry, NIDDK, National Institutes of Health, 8 Center Dr., Bethesda, MD 20892, USA. Y1 - 2005/02/18/ PY - 2005 DA - 2005 Feb 18 SP - 5664 EP - 5675 VL - 280 IS - 7 SN - 0021-9258, 0021-9258 KW - Ligands KW - 0 KW - Phosphatidylinositols KW - Receptor, Muscarinic M3 KW - Tyrosine KW - 42HK56048U KW - Arginine KW - 94ZLA3W45F KW - Index Medicus KW - Point Mutation -- genetics KW - Animals KW - Phosphatidylinositols -- metabolism KW - Arginine -- metabolism KW - COS Cells KW - Suppression, Genetic -- genetics KW - Radioligand Assay KW - Hydrolysis KW - Structure-Activity Relationship KW - Rats KW - Alleles KW - Arginine -- genetics KW - Tyrosine -- genetics KW - Tyrosine -- metabolism KW - Saccharomyces cerevisiae -- genetics KW - Receptor, Muscarinic M3 -- metabolism KW - Mutagenesis -- genetics KW - Receptor, Muscarinic M3 -- chemistry KW - Receptor, Muscarinic M3 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67424745?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Random+mutagenesis+of+the+M3+muscarinic+acetylcholine+receptor+expressed+in+yeast%3A+identification+of+second-site+mutations+that+restore+function+to+a+coupling-deficient+mutant+M3+receptor.&rft.au=Li%2C+Bo%3BNowak%2C+Nicola+M%3BKim%2C+Soo-Kyung%3BJacobson%2C+Kenneth+A%3BBagheri%2C+Ali%3BSchmidt%2C+Clarice%3BWess%2C+J%C3%BCrgen&rft.aulast=Li&rft.aufirst=Bo&rft.date=2005-02-18&rft.volume=280&rft.issue=7&rft.spage=5664&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-18 N1 - Date created - 2005-02-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Toll-like receptor 4 on nonhematopoietic cells sustains CNS inflammation during endotoxemia, independent of systemic cytokines. AN - 67440036; 15716415 AB - Inflammatory agonists such as lipopolysaccharide (LPS) induce robust systemic as well as CNS responses after peripheral administration. Responses in the innate immune system require triggering of toll-like receptor 4 (TLR4), but the origin of CNS sequelas has been controversial. We demonstrate expression of TLR4 transcripts in mouse brain in the meninges, ventricular ependyma, circumventricular organs, along the vasculature, and in parenchymal microglia. The contribution of TLR4 expressed in CNS resident versus hematopoietic cells to the development of CNS inflammation was examined using chimeric mice. Reciprocal bone marrow chimeras between wild-type and TLR4 mutant mice show that TLR4 on CNS resident cells is critically required for sustained inflammation in the brain after systemic LPS administration. Hematopoietic TLR4 alone supported the systemic release of acute phase cytokines, but transcription of proinflammatory genes in the CNS was reduced in duration. In contrast, TLR4 function in radiation-resistant cells was sufficient for inflammatory progression in the brains of chimeric mice, despite the striking absence of cytokine elevations in serum. Surprisingly, a temporal rise in serum corticosterone was also dependent on TLR4 signaling in nonhematopoietic cells. Our findings demonstrate a requirement for TLR4 function in CNS-resident cells, independent of systemic cytokine effects, for sustained CNS-specific inflammation and corticosterone rise during endotoxemia. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Chakravarty, Sumana AU - Herkenham, Miles AD - Section on Functional Neuroanatomy, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA. Y1 - 2005/02/16/ PY - 2005 DA - 2005 Feb 16 SP - 1788 EP - 1796 VL - 25 IS - 7 KW - Cx3cr1 protein, mouse KW - 0 KW - Cytokines KW - I-kappa B Proteins KW - Lipopolysaccharides KW - Nfkbia protein, mouse KW - RNA, Messenger KW - Receptors, Chemokine KW - Receptors, Immunologic KW - Tlr4 protein, mouse KW - Toll-Like Receptor 4 KW - lipopolysaccharide, E coli O55-B5 KW - NF-KappaB Inhibitor alpha KW - 139874-52-5 KW - Corticosterone KW - W980KJ009P KW - Index Medicus KW - Animals KW - Cell Lineage KW - Spleen -- metabolism KW - Hypothalamo-Hypophyseal System -- physiopathology KW - Meninges -- metabolism KW - Receptors, Chemokine -- genetics KW - Mice KW - RNA, Messenger -- genetics KW - RNA, Messenger -- biosynthesis KW - Receptors, Chemokine -- biosynthesis KW - Choroid Plexus -- metabolism KW - Corticosterone -- blood KW - Pituitary-Adrenal System -- physiopathology KW - Mice, Inbred C57BL KW - Mice, Inbred C3H KW - Cytokines -- physiology KW - Lipopolysaccharides -- toxicity KW - Gene Expression Regulation KW - I-kappa B Proteins -- genetics KW - I-kappa B Proteins -- biosynthesis KW - Radiation Chimera KW - Bone Marrow Transplantation KW - Male KW - Receptors, Immunologic -- genetics KW - Endotoxemia -- pathology KW - Encephalitis -- pathology KW - Endotoxemia -- complications KW - Receptors, Immunologic -- physiology KW - Encephalitis -- etiology KW - Brain -- metabolism KW - Receptors, Immunologic -- biosynthesis KW - Encephalitis -- metabolism KW - Receptors, Immunologic -- deficiency KW - Endotoxemia -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67440036?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Toll-like+receptor+4+on+nonhematopoietic+cells+sustains+CNS+inflammation+during+endotoxemia%2C+independent+of+systemic+cytokines.&rft.au=Chakravarty%2C+Sumana%3BHerkenham%2C+Miles&rft.aulast=Chakravarty&rft.aufirst=Sumana&rft.date=2005-02-16&rft.volume=25&rft.issue=7&rft.spage=1788&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-02-17 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Epilepsy Curr. 2006 Jan-Feb;6(1):11-3 [16477316] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Zinc concentration in esophageal biopsy specimens measured by x-ray fluorescence and esophageal cancer risk. AN - 67438091; 15713965 AB - In rodents, zinc deficiency potentiates the effects of certain nitrosamines that act as esophageal carcinogens. Studies of the association between zinc and esophageal squamous cell carcinoma in humans have been hampered by plasma zinc homeostasis, which obscures individual differences in total zinc stores, and by the uncertainty regarding zinc bioavailability when estimating dietary zinc intake because phytate from whole grains effectively prohibits zinc absorption. By using baseline tissue biopsy specimens collected in a prospective observational study, we determined the association between incident esophageal squamous cell carcinoma and baseline element concentrations in tissue sections from residents of Linzhou, China, participating in a nutrition intervention trial. We used x-ray fluorescence spectroscopy to measure zinc, copper, iron, nickel, and sulfur concentrations in single 5-microm-thick sections from formalin-fixed, paraffin-embedded esophageal biopsy specimens collected in 1985 from 60 eventual case and 72 control subjects. Subjects were matched on baseline histology and followed for 16 years. We used Cox proportional hazards models to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) for the association between each element and risk of incident esophageal cancer. All statistical tests were two-sided. The risk of developing esophageal cancer was much lower for subjects in the highest quartile of esophageal tissue zinc concentration compared with those in the lowest quartile (HR = 0.21, 95% CI = 0.065 to 0.68). The association was statistically significant across quartiles (P(trend) = .015). Individuals in the highest quartile of sulfur concentration had a lower risk of esophageal cancer than individuals in the lowest quartile (HR = 0.29, 95% CI = 0.095 to 0.85), but the association across quartiles was not statistically significant (P(trend) = .081). There was no association between copper, iron, or nickel concentrations and risk of esophageal cancer. High tissue zinc concentration was strongly associated with a reduced risk of developing esophageal squamous cell carcinoma. X-ray fluorescence spectroscopy can be used to assess relationships among concentrations of both nutritional and toxic elements and disease risk in banked tissue specimens. JF - Journal of the National Cancer Institute AU - Abnet, Christian C AU - Lai, Barry AU - Qiao, You-Lin AU - Vogt, Stefan AU - Luo, Xian-Mao AU - Taylor, Philip R AU - Dong, Zhi-Wei AU - Mark, Steven D AU - Dawsey, Sanford M AD - Cancer Prevention Studies Branch, National Cancer Institute, National Institutes of Health, 6116 Executive Blvd., Rm. 705, Bethesda, MD 20892, USA. abnetc@mail.nih.gov Y1 - 2005/02/16/ PY - 2005 DA - 2005 Feb 16 SP - 301 EP - 306 VL - 97 IS - 4 KW - Sulfur KW - 70FD1KFU70 KW - Copper KW - 789U1901C5 KW - Nickel KW - 7OV03QG267 KW - Iron KW - E1UOL152H7 KW - Zinc KW - J41CSQ7QDS KW - Index Medicus KW - Odds Ratio KW - Iron -- analysis KW - Spectrometry, X-Ray Emission KW - Humans KW - Nickel -- analysis KW - Biopsy KW - China -- epidemiology KW - Case-Control Studies KW - Confidence Intervals KW - Incidence KW - Copper -- analysis KW - Sulfur -- analysis KW - Proportional Hazards Models KW - Zinc -- analysis KW - Carcinoma, Squamous Cell -- epidemiology KW - Carcinoma, Squamous Cell -- pathology KW - Esophageal Neoplasms -- chemistry KW - Carcinoma, Squamous Cell -- chemistry KW - Esophageal Neoplasms -- pathology KW - Esophageal Neoplasms -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67438091?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Zinc+concentration+in+esophageal+biopsy+specimens+measured+by+x-ray+fluorescence+and+esophageal+cancer+risk.&rft.au=Abnet%2C+Christian+C%3BLai%2C+Barry%3BQiao%2C+You-Lin%3BVogt%2C+Stefan%3BLuo%2C+Xian-Mao%3BTaylor%2C+Philip+R%3BDong%2C+Zhi-Wei%3BMark%2C+Steven+D%3BDawsey%2C+Sanford+M&rft.aulast=Abnet&rft.aufirst=Christian&rft.date=2005-02-16&rft.volume=97&rft.issue=4&rft.spage=301&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=1460-2105&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-02-22 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Taxanes with anthracyclines as first-line chemotherapy for metastatic breast carcinoma. AN - 67435382; 15637696 AB - The magnitude of the benefit of adding taxanes to anthracyclines in first-line chemotherapy for metastatic breast carcinoma is still unclear. The authors performed a pooled analysis as well as a meta-analysis of all Phase III trials, to determine whether the combination of anthracyclines plus taxanes provided an advantage over standard anthracyclines-based regimens. All Phase III peer-reviewed published or presented trials were considered eligible. A pooled analysis (Method A) and a literature-based meta-analysis (Method B) were accomplished, and event-based relative risk ratios (RR(A-B)) with 95% confidence intervals were derived. Both analyses were performed to examine for significant differences in time to disease progression (TTP), overall response rate (ORR), overall survival (OS), complete response rate (CR), neutropenia, and febrile neutropenia (FN). For both analyses, a heterogeneity test was applied. Seven trials (2805 patients) were gathered. When data were pooled and plotted, significant differences in favor of taxanes were seen for ORR (RR(A-B) 1.21, P<0.001), CR (RR(A) 2.04; RR(B) 1.81, P<0.001), even though they caused a significant increase in neutropenia (RR(A) 1.19; RR(B) 1.15, P<0.001) and FN (RR(A) 2.82; RR(B) 3.44, P<0.001). A borderline significance in favor of taxanes was seen in TTP (RR(A) 1.10, P=0.05; RR(B) 1.06, P=0.07). A nonsignificant trend for taxanes was found in OS. No significant heterogeneity (except for neutropenia, P<0.01) was observed. The adjunction of taxanes to anthracyclines in first-line chemotherapy for metastatic breast carcinoma yielded a significant benefit in activity (ORR, CR), a slight advantage in TTP, and a trend in OS, although with a significant cost in hematologic toxicity. Copyright (c) 2005 American Cancer Society. JF - Cancer AU - Bria, Emilio AU - Giannarelli, Diana AU - Felici, Alessandra AU - Peters, William P AU - Nisticò, Cecilia AU - Vanni, Barbara AU - Cuppone, Federica AU - Cognetti, Francesco AU - Terzoli, Edmondo AD - Department of Medical Oncology, Regina Elena National Cancer Institute, Rome, Italy. emiliobria@yahoo.it Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 672 EP - 679 VL - 103 IS - 4 SN - 0008-543X, 0008-543X KW - Anthracyclines KW - 0 KW - Antineoplastic Agents KW - Taxoids KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Taxoids -- adverse effects KW - Clinical Trials as Topic KW - Anthracyclines -- adverse effects KW - Survival Analysis KW - Antineoplastic Agents -- adverse effects KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- mortality KW - Antineoplastic Combined Chemotherapy Protocols -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67435382?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer&rft.atitle=Taxanes+with+anthracyclines+as+first-line+chemotherapy+for+metastatic+breast+carcinoma.&rft.au=Bria%2C+Emilio%3BGiannarelli%2C+Diana%3BFelici%2C+Alessandra%3BPeters%2C+William+P%3BNistic%C3%B2%2C+Cecilia%3BVanni%2C+Barbara%3BCuppone%2C+Federica%3BCognetti%2C+Francesco%3BTerzoli%2C+Edmondo&rft.aulast=Bria&rft.aufirst=Emilio&rft.date=2005-02-15&rft.volume=103&rft.issue=4&rft.spage=672&rft.isbn=&rft.btitle=&rft.title=Cancer&rft.issn=0008543X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-29 N1 - Date created - 2005-02-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins. AN - 67433072; 15701688 AB - The Golgi-localized, gamma-ear-containing, Arf (ADP-ribosylation factor)-binding (GGA) proteins are clathrin adaptors that mediate the sorting of transmembrane-cargo molecules at the trans-Golgi network and endosomes. Cargo proteins can be directed into the GGA pathway by at least two different types of sorting signals: acidic cluster-dileucine motifs and covalent modification by ubiquitin. The latter modification is recognized by the GGAs through binding to their GAT [GGA and TOM (target of Myb)] domain. Here we report the crystal structure of the GAT domain of human GGA3 in a 1:1 complex with ubiquitin at 2.8-A resolution. Ubiquitin binds to a hydrophobic and acidic patch on helices alpha1 and alpha2 of the GAT three-helix bundle that includes Asn-223, Leu-227, Glu-230, Met-231, Asp-244, Glu-246, Leu-247, Glu-250, and Leu-251. The GAT-binding surface on ubiquitin is a hydrophobic patch centered on Ile-44 that is also responsible for binding most other ubiquitin effectors. The ubiquitin-binding site observed in the crystal is distinct from the Rabaptin-5-binding site on helices alpha2 and alpha3 of the GAT domain. Mutational analysis and modeling of the ubiquitin-Rabaptin-5-GAT ternary complex indicates that ubiquitin and Rabaptin-5 can bind to the GAT domain at two different sites without any steric conflict. This ability highlights the GAT domain as a hub for interactions with multiple partners in trafficking. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Prag, Gali AU - Lee, Sangho AU - Mattera, Rafael AU - Arighi, Cecilia N AU - Beach, Bridgette M AU - Bonifacino, Juan S AU - Hurley, James H AD - Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 2334 EP - 2339 VL - 102 IS - 7 SN - 0027-8424, 0027-8424 KW - Adaptor Proteins, Vesicular Transport KW - 0 KW - GGA adaptor proteins KW - Multiprotein Complexes KW - RABEP1 protein, human KW - Recombinant Proteins KW - Ubiquitin KW - Vesicular Transport Proteins KW - ADP-Ribosylation Factors KW - EC 3.6.5.2 KW - Index Medicus KW - Molecular Structure KW - Models, Molecular KW - Humans KW - Amino Acid Sequence KW - Recombinant Proteins -- genetics KW - Binding Sites KW - Static Electricity KW - Mutagenesis, Site-Directed KW - Ubiquitin -- metabolism KW - Ubiquitin -- chemistry KW - Recombinant Proteins -- metabolism KW - In Vitro Techniques KW - Molecular Sequence Data KW - Crystallography, X-Ray KW - Vesicular Transport Proteins -- metabolism KW - Recombinant Proteins -- chemistry KW - Sequence Homology, Amino Acid KW - Protein Structure, Tertiary KW - Golgi Apparatus -- metabolism KW - Vesicular Transport Proteins -- chemistry KW - Protein Conformation KW - ADP-Ribosylation Factors -- genetics KW - ADP-Ribosylation Factors -- metabolism KW - Adaptor Proteins, Vesicular Transport -- metabolism KW - Adaptor Proteins, Vesicular Transport -- chemistry KW - ADP-Ribosylation Factors -- chemistry KW - Adaptor Proteins, Vesicular Transport -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67433072?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Structural+mechanism+for+ubiquitinated-cargo+recognition+by+the+Golgi-localized%2C+gamma-ear-containing%2C+ADP-ribosylation-factor-binding+proteins.&rft.au=Prag%2C+Gali%3BLee%2C+Sangho%3BMattera%2C+Rafael%3BArighi%2C+Cecilia+N%3BBeach%2C+Bridgette+M%3BBonifacino%2C+Juan+S%3BHurley%2C+James+H&rft.aulast=Prag&rft.aufirst=Gali&rft.date=2005-02-15&rft.volume=102&rft.issue=7&rft.spage=2334&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-13 N1 - Date created - 2005-02-16 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1YD8; PDB N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):861-6 [8570649] J Mol Biol. 1993 Dec 20;234(4):946-50 [8263940] Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21 [9757107] Protein Expr Purif. 1999 Feb;15(1):34-9 [10024467] EMBO J. 2004 Nov 10;23(22):4371-83 [15496985] J Biol Chem. 2004 Dec 24;279(52):54808-16 [15494413] Nat Rev Mol Cell Biol. 2001 Mar;2(3):195-201 [11265249] Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1127-34 [11468396] J Biol Chem. 2001 Aug 10;276(32):30483-9 [11399765] Nat Rev Mol Cell Biol. 2002 Dec;3(12):893-905 [12461556] EMBO J. 2003 Jan 2;22(1):78-88 [12505986] Dev Cell. 2003 Mar;4(3):321-32 [12636914] Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4451-6 [12668765] Nat Struct Biol. 2003 May;10(5):386-93 [12679809] Biochemistry. 2003 Jun 3;42(21):6392-9 [12767220] Nat Rev Mol Cell Biol. 2003 Jun;4(6):491-7 [12778128] Cell. 2003 May 30;113(5):609-20 [12787502] Cell. 2003 May 30;113(5):621-30 [12787503] EMBO J. 2003 Sep 15;22(18):4597-606 [12970172] Trends Biochem Sci. 2003 Nov;28(11):598-603 [14607090] Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2237-41 [14646082] J Biol Chem. 2003 Dec 26;278(52):52865-72 [14563850] Nat Rev Mol Cell Biol. 2004 Jan;5(1):23-32 [14708007] J Biol Chem. 2004 Feb 20;279(8):7105-11 [14660606] Nat Cell Biol. 2004 Mar;6(3):244-51 [15039775] Nat Cell Biol. 2004 Mar;6(3):252-9 [15039776] Mol Cell. 2004 Mar 26;13(6):783-9 [15053872] EMBO J. 2004 Apr 7;23(7):1411-21 [15029239] J Biol Chem. 2004 Jun 4;279(23):24435-43 [15047686] Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W606-9 [15215460] J Biol Chem. 2004 Jul 2;279(27):28689-96 [15044434] J Biol Chem. 2004 Jul 23;279(30):31409-18 [15143060] EMBO J. 2004 Oct 1;23(19):3701-10 [15359277] EMBO J. 2004 Oct 13;23(20):3909-17 [15457209] J Biol Chem. 2004 Oct 29;279(44):46191-203 [15292237] Eur J Cell Biol. 2004 Jul;83(6):257-62 [15511083] Acta Crystallogr A. 1991 Mar 1;47 ( Pt 2):110-9 [2025413] Methods Enzymol. 1997;276:344-58 [9048378] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - TGF-beta and vitamin D3 utilize distinct pathways to suppress IL-12 production and modulate rapid differentiation of human monocytes into CD83+ dendritic cells. AN - 67413126; 15699136 AB - We previously demonstrated that agents known to signal infection or inflammation can rapidly and directly drive differentiation of human CD14+ monocytes into CD83+ dendritic cells (DCs) when introduced to cells under serum-free conditions. In this study, we evaluated the effects of TGF-beta and vitamin D3 (VitD3) on the proportion and function of monocytes that adopt DC characteristics. TGF-beta significantly decreased the proportion of cells that rapidly adopted stable DC characteristics in response to LPS, but had little or no effect on calcium ionophore-induced differentiation. In contrast, VitD3 showed no such pathway specificity and dramatically suppressed differentiation of monocytes into DCs in response to these agents. Both TGF-beta and VitD3 altered cytokine and chemokine production in LPS-treated monocytes, inhibited IL-12 and IL-10 secretion, and decreased the functional capacity of DCs. Despite the similar effects of TGF-beta and VitD3, there are significant differences in the signaling pathways used by these agents, as evidenced by their distinct effects on LPS- and calcium ionophore-induced DC differentiation, on LPS-induced secretion of IL-10, and on two members of the NF-kappaB family of transcription factors, RelB and cRel. These studies identify TGF-beta and VitD3 as potent regulatory factors that use distinct pathways to suppress both the differentiation of DCs as well as their capacity to secrete the Th1-polarizing cytokine IL-12. Because these agents are present in serum and negatively affect DC differentiation at physiological concentrations, our findings are likely to have significance regarding the in vivo role of TGF-beta and VitD3 in determining the type of immune responses. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Lyakh, Lyudmila A AU - Sanford, Michael AU - Chekol, Sebel AU - Young, Howard A AU - Roberts, Anita B AD - Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, Bethesda, MD 20892, USA. Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 2061 EP - 2070 VL - 174 IS - 4 SN - 0022-1767, 0022-1767 KW - Antigens, CD KW - 0 KW - CD83 antigen KW - Culture Media, Serum-Free KW - DNA-Binding Proteins KW - Immunoglobulins KW - Immunologic Factors KW - Lipopolysaccharides KW - Membrane Glycoproteins KW - NF-kappa B KW - Receptors, Cell Surface KW - Smad Proteins KW - Toll-Like Receptors KW - Trans-Activators KW - Transforming Growth Factor beta KW - Interleukin-12 KW - 187348-17-0 KW - Cholecalciferol KW - 1C6V77QF41 KW - p38 Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Abridged Index Medicus KW - Index Medicus KW - Trans-Activators -- metabolism KW - Lipopolysaccharides -- antagonists & inhibitors KW - Humans KW - Lipopolysaccharides -- pharmacology KW - Lymphocyte Culture Test, Mixed KW - Down-Regulation -- immunology KW - Receptors, Cell Surface -- biosynthesis KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Cell Differentiation -- immunology KW - Active Transport, Cell Nucleus -- immunology KW - p38 Mitogen-Activated Protein Kinases -- antagonists & inhibitors KW - Monocytes -- cytology KW - Cells, Cultured KW - Monocytes -- immunology KW - Monocytes -- metabolism KW - Antigen-Presenting Cells -- immunology KW - Immunophenotyping KW - NF-kappa B -- metabolism KW - NF-kappa B -- antagonists & inhibitors KW - DNA-Binding Proteins -- metabolism KW - Dendritic Cells -- immunology KW - Transforming Growth Factor beta -- physiology KW - Membrane Glycoproteins -- biosynthesis KW - Immunologic Factors -- physiology KW - Dendritic Cells -- metabolism KW - Cholecalciferol -- physiology KW - Interleukin-12 -- biosynthesis KW - Signal Transduction -- immunology KW - Immunoglobulins -- biosynthesis KW - Interleukin-12 -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67413126?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=TGF-beta+and+vitamin+D3+utilize+distinct+pathways+to+suppress+IL-12+production+and+modulate+rapid+differentiation+of+human+monocytes+into+CD83%2B+dendritic+cells.&rft.au=Lyakh%2C+Lyudmila+A%3BSanford%2C+Michael%3BChekol%2C+Sebel%3BYoung%2C+Howard+A%3BRoberts%2C+Anita+B&rft.aulast=Lyakh&rft.aufirst=Lyudmila&rft.date=2005-02-15&rft.volume=174&rft.issue=4&rft.spage=2061&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-29 N1 - Date created - 2005-02-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Identification of key amino acids in the gastrin-releasing peptide receptor (GRPR) responsible for high affinity binding of gastrin-releasing peptide (GRP). AN - 67379839; 15670577 AB - The bombesin (Bn) receptor family includes the gastrin-releasing peptide (GRPR) and neuromedin B (NMBR) receptors, Bn receptor subtype 3 (BRS-3) and Bn receptor subtype 4 (BB(4)). They share 50% homology, yet their affinities for gastrin-releasing peptide (GRP) differ. The determinants of GRP high affinity for GRPR and BB(4), and low affinity for BRS-3 are largely unknown. To address this question we made an analysis of structural homologies in Bn receptor members correlated with their affinities for GRP to develop criteria to identify amino acids important for GRP selectivity. Fourteen differences were identified and each was mutated singly in GRPR to that found in hBRS-3. Eleven mutants had a loss of GRP affinity. Furthermore, three of four amino acids in the GRPR selected used a similar approach and previously reported to be important for high affinity Bn binding, were important for GRP affinity. Some GRPR mutants containing combinations of these mutations had greater decreases in GRP affinity than any single mutation. Particularly important for GRP selectivity were K101, Q121, A198, P199, S293, R288, T297 in GRPR. These results were confirmed by making the reverse mutations in BRS-3 to make GRP gain of affinity mutants. Modeling studies demonstrated a number of the important amino acids had side-chains oriented inward and within 6A of the binding pocket. These results demonstrated this approach could identify amino acids needed for GRP affinity and complemented results from chimera/mutagenesis studies by identifying which differences in the extracellular domains of Bn receptors were important for GRP affinity. JF - Biochemical pharmacology AU - Nakagawa, Tomoo AU - Hocart, Simon J AU - Schumann, Michael AU - Tapia, Jose A AU - Mantey, Samuel A AU - Coy, David H AU - Tokita, Kenji AU - Katsuno, Tatsuro AU - Jensen, Robert T AD - Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD 20892-1804, USA. Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 579 EP - 593 VL - 69 IS - 4 SN - 0006-2952, 0006-2952 KW - Receptors, Bombesin KW - 0 KW - Gastrin-Releasing Peptide KW - 80043-53-4 KW - Index Medicus KW - Animals KW - BALB 3T3 Cells KW - Models, Molecular KW - Molecular Sequence Data KW - Mice KW - Amino Acid Sequence KW - Structure-Activity Relationship KW - Binding Sites KW - Gastrin-Releasing Peptide -- metabolism KW - Receptors, Bombesin -- metabolism KW - Receptors, Bombesin -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67379839?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemical+pharmacology&rft.atitle=Identification+of+key+amino+acids+in+the+gastrin-releasing+peptide+receptor+%28GRPR%29+responsible+for+high+affinity+binding+of+gastrin-releasing+peptide+%28GRP%29.&rft.au=Nakagawa%2C+Tomoo%3BHocart%2C+Simon+J%3BSchumann%2C+Michael%3BTapia%2C+Jose+A%3BMantey%2C+Samuel+A%3BCoy%2C+David+H%3BTokita%2C+Kenji%3BKatsuno%2C+Tatsuro%3BJensen%2C+Robert+T&rft.aulast=Nakagawa&rft.aufirst=Tomoo&rft.date=2005-02-15&rft.volume=69&rft.issue=4&rft.spage=579&rft.isbn=&rft.btitle=&rft.title=Biochemical+pharmacology&rft.issn=00062952&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-07 N1 - Date created - 2005-01-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Analyses of Recombinant Vaccinia and Fowlpox Vaccine Vectors Expressing Transgenes for Two Human Tumor Antigens and Three Human Costimulatory Molecules AN - 19929553; 6188066 AB - PURPOSE: The poor immunogenicity of tumor antigens and the antigenic heterogeneity of tumors call for vaccine strategies to enhance T-cell responses to multiple antigens. Two antigens expressed noncoordinately on most human carcinomas are carcinoembryonic antigen (CEA) and MUC-1. We report here the construction and characterization of two viral vector vaccines to address these issues. Experimental Design: The two viral vectors analyzed are the replication- competent recombinant vaccinia virus (rV-) and the avipox vector, fowlpox (rF-), which is replication incompetent in mammalian cells. Each vector encodes the transgenes for three human costimulatory molecules (B7-1, ICAM-1, and LFA-3, designated TRICOM) and the CEA and MUC-1 transgenes (which also contain agonist epitopes). The vectors are designated rV-CEA/MUC/TRICOM and rF-CEA/MUC/TRICOM. RESULTS: Each of the vectors is shown to be capable of faithfully expressing all five transgenes in human dendritic cells (DC). DCs infected with either vector are shown to activate both CEA-and MUC-1-specific T-cell lines to the same level as DCs infected with CEA-TRICOM or MUC-1-TRICOM vectors. Thus, no evidence of antigenic competition between CEA and MUC-1 was observed. Human DCs infected with rV-CEA/MUC/TRICOM or rF-CEA/MUC/TRICOM are also shown to be capable of generating both MUC-1-and CEA-specific T-cell lines; these T-cell lines are in turn shown to be capable of lysing targets pulsed with MUC-1 or CEA peptides as well as human tumor cells endogenously expressing MUC-1 and/or CEA. CONCLUSION: These studies provide the rationale for the clinical evaluation of these multigene vectors in patients with a range of carcinomas expressing MUC-1 and/or CEA. JF - Clinical Cancer Research AU - Tsang, Kwong Y AU - Palena, Claudia AU - Yokokawa, Junko AU - Arlen, Philip M AU - Gulley, James L AU - Mazzara, Gail P AU - Gritz, Linda AU - Gomez Yafal, Alicia AU - Ogueta, Sandra AU - Greenhalgh, Patricia AU - Manson, Kelledy AU - Panicali, Dennis AU - Schlom, Jeffrey AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland and Therion Biologics Corporation, Cambridge, Massachusetts Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 1597 EP - 1607 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 4 SN - 1078-0432, 1078-0432 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - Fowlpox KW - Vaccinia KW - Replication KW - Carcinoembryonic antigen KW - Transgenes KW - CD58 antigen KW - Tumors KW - Tumor cells KW - Carcinoma KW - Antigenic competition KW - Costimulator KW - Dendritic cells KW - B7-1 antigen KW - Vaccinia virus KW - Mammalian cells KW - Immunogenicity KW - Antigen (tumor-associated) KW - intercellular adhesion molecule 1 KW - Lymphocytes T KW - Vaccines KW - Epitopes KW - V 22350:Immunology KW - F 06915:Cancer Immunology KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19929553?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=Analyses+of+Recombinant+Vaccinia+and+Fowlpox+Vaccine+Vectors+Expressing+Transgenes+for+Two+Human+Tumor+Antigens+and+Three+Human+Costimulatory+Molecules&rft.au=Tsang%2C+Kwong+Y%3BPalena%2C+Claudia%3BYokokawa%2C+Junko%3BArlen%2C+Philip+M%3BGulley%2C+James+L%3BMazzara%2C+Gail+P%3BGritz%2C+Linda%3BGomez+Yafal%2C+Alicia%3BOgueta%2C+Sandra%3BGreenhalgh%2C+Patricia%3BManson%2C+Kelledy%3BPanicali%2C+Dennis%3BSchlom%2C+Jeffrey&rft.aulast=Tsang&rft.aufirst=Kwong&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1597&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Fowlpox; Replication; Vaccinia; Transgenes; Carcinoembryonic antigen; CD58 antigen; Tumors; Tumor cells; Antigenic competition; Carcinoma; B7-1 antigen; Dendritic cells; Costimulator; Mammalian cells; Immunogenicity; intercellular adhesion molecule 1; Antigen (tumor-associated); Lymphocytes T; Vaccines; Epitopes; Vaccinia virus ER - TY - JOUR T1 - HA22 (R490A) Is a Recombinant Immunotoxin with Increased Antitumor Activity without an Increase in Animal Toxicity AN - 19820675; 6188060 AB - PURPOSE: RFB4 (dsFv)-PE38 (BL22) is a recombinant immunotoxin containing an anti-CD22 (Fv) fused to truncated Pseudomonas exotoxin A, which induces a high complete remission rate in patients with purine analogue-resistant hairy cell leukemia. HA22 is a mutant of BL22 with mutations in heavy-chain CDR3 resulting in increased cytotoxic activity. Our goal was to improve the activity of HA22. Experimental Design: Arg super(490), which is located in the catalytic domain (III) of the immunotoxin HA22, was mutated to alanine. Purified immunotoxins were produced and tested for cytotoxic activity in cell culture and for antitumor activity and nonspecific toxicity in mice. ADP-ribosylation activity was also measured. RESULTS: HA22 (R490A) is [approx]2-fold more cytotoxic than HA22 on several CD22-positive cell lines. When injected i.v., HA22 (R490A) has more potent antitumor activity than HA22 against CA46 tumors in mice. HA22 and HA22 (R490A) have similar LD sub(50)s ([approx]1.3 mg/kg) and similar plasma half- lives. The R490A mutation also improved the cytotoxicity of the antimesothelin recombinant immunotoxin SS1 (dsFv)-PE38 (SS1P). In vitro ADP-ribosylation assays show that HA22 R490A has increased activity. Increased cytotoxic activity is probably related to this increase in ADP-ribosylation activity. CONCLUSION: Protein engineering can be used to increase the efficacy of recombinant immunotoxins. Because HA22 (R490A) has increased antitumor activity without increased animal toxicity, immunotoxins with this mutation are candidates for clinical development. JF - Clinical Cancer Research AU - Bang, Sookhee AU - Nagata, Satoshi AU - Onda, Masanori AU - Kreitman, Robert J AU - Pastan, Ira AD - Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 1545 EP - 1550 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 11 IS - 4 SN - 1078-0432, 1078-0432 KW - Toxicology Abstracts; Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - complementarity-determining region 3 KW - Alanine KW - Protein engineering KW - Remission KW - Pseudomonas KW - Cell culture KW - Toxicity KW - Tumors KW - exotoxin A KW - Fv KW - purines KW - Immunotoxins KW - Cytotoxicity KW - Mutation KW - ADP-ribosylation KW - Hairy cell leukemia KW - Antitumor activity KW - X 24310:Pharmaceuticals KW - F 06915:Cancer Immunology KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19820675?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+Cancer+Research&rft.atitle=HA22+%28R490A%29+Is+a+Recombinant+Immunotoxin+with+Increased+Antitumor+Activity+without+an+Increase+in+Animal+Toxicity&rft.au=Bang%2C+Sookhee%3BNagata%2C+Satoshi%3BOnda%2C+Masanori%3BKreitman%2C+Robert+J%3BPastan%2C+Ira&rft.aulast=Bang&rft.aufirst=Sookhee&rft.date=2005-02-15&rft.volume=11&rft.issue=4&rft.spage=1545&rft.isbn=&rft.btitle=&rft.title=Clinical+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - complementarity-determining region 3; Protein engineering; Alanine; Remission; Cell culture; Tumors; Toxicity; exotoxin A; Immunotoxins; purines; Fv; Cytotoxicity; Hairy cell leukemia; ADP-ribosylation; Mutation; Antitumor activity; Pseudomonas ER - TY - JOUR T1 - Serologic Correlates of Protection against Enterotoxigenic Escherichia coli Diarrhea AN - 17802796; 6160874 AB - We conducted a nested case-control study in 397 rural Egyptian children <36 months of age to assess the correlation between serum levels of antibodies against toxin and colonization factors (CFs) and the risk of homologous enterotoxigenic Escherichia coli (ETEC) diarrhea. Active case detection was performed via semiweekly home visits, and blood was obtained at 3-month intervals. After each serosurvey, case subjects were selected from children experiencing a CF antigen (CFA)/I-, CFA/II-, CFA/IV-, or heat-labile enterotoxin (LT)-ETEC diarrheal episode during the subsequent 3 months. Up to 5 control subjects per case subject were selected from children who did not experience an ETEC diarrheal episode during the corresponding interval. Serum titers of immunoglobulin G antibodies against CFA/I, coli surface antigen (CS) 3, CS6, and LT were measured by enzyme-linked immunosorbant assay. The distribution of serum titers of LT, CS3, and CS6 antibodies did not differ between the case and control subjects. For children <18 months of age, serum titers of CFA/I antibody were inversely related to the risk of CFA/I-ETEC diarrhea; reciprocal serum titers of CFA/I antibody greater than or equal to 76 were associated with a 77% reduction in the odds of CFA/I-ETEC diarrhea. Induction of reciprocal serum titers of antibodies against CFA/I within or above the 76-186 range should be further evaluated as a predictor for assessment of the ability of candidate vaccines to protect against CFA/I-ETEC diarrhea. JF - Journal of Infectious Diseases AU - Rao, M R AU - Wierzba, T F AU - Savarino, S J AU - Abu-Elyazeed, R AU - El-Ghoreb, N AU - Hall, E R AU - Naficy, A AU - Abdel-Messih, I AU - Frenck, RW Jr AU - Svennerholm, A-M AU - Clemens, J D AD - Division of Epidemiology, Statistics, and Prevention Research, National Institute of Child Health and Human Development, Bethesda, MD, USA Y1 - 2005/02/15/ PY - 2005 DA - 2005 Feb 15 SP - 562 EP - 570 VL - 191 IS - 4 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology KW - Serum levels KW - Blood KW - Diarrhea KW - surface antigens KW - Immunoglobulin G KW - Escherichia coli KW - Vaccines KW - Children KW - Toxins KW - Colonization factor KW - heat-labile enterotoxin KW - J 02846:Gastrointestinal tract UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17802796?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Serologic+Correlates+of+Protection+against+Enterotoxigenic+Escherichia+coli+Diarrhea&rft.au=Rao%2C+M+R%3BWierzba%2C+T+F%3BSavarino%2C+S+J%3BAbu-Elyazeed%2C+R%3BEl-Ghoreb%2C+N%3BHall%2C+E+R%3BNaficy%2C+A%3BAbdel-Messih%2C+I%3BFrenck%2C+RW+Jr%3BSvennerholm%2C+A-M%3BClemens%2C+J+D&rft.aulast=Rao&rft.aufirst=M&rft.date=2005-02-15&rft.volume=191&rft.issue=4&rft.spage=562&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Diarrhea; Children; Serum levels; Colonization factor; Blood; heat-labile enterotoxin; Vaccines; Toxins; surface antigens; Immunoglobulin G ER - TY - JOUR T1 - DLC-1, a Rho GTPase-activating protein with tumor suppressor function, is essential for embryonic development. AN - 67426838; 15710412 AB - DLC-1 (deleted in liver cancer 1) is a Rho GTPase-activating protein that is able to inhibit cell growth and suppress tumorigenesis. We have used homologous recombination to inactivate the mouse DLC-1 gene (Arhgap7). Mice heterozygous for the targeted allele were phenotypically normal, but homozygous mutant embryos did not survive beyond 10.5 days post coitum. Histological analysis revealed that DLC-1-/- embryos had defects in the neural tube, brain, heart, and placenta. Cultured fibroblasts from DLC-1-deficient embryos displayed alterations in the organization of actin filaments and focal adhesions. JF - FEBS letters AU - Durkin, Marian E AU - Avner, Miriam R AU - Huh, Chang-Goo AU - Yuan, Bao-Zhu AU - Thorgeirsson, Snorri S AU - Popescu, Nicholas C AD - Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA. marian_durkin@nih.gov Y1 - 2005/02/14/ PY - 2005 DA - 2005 Feb 14 SP - 1191 EP - 1196 VL - 579 IS - 5 SN - 0014-5793, 0014-5793 KW - DLC-1 (deleted in liver cancer) protein, mouse KW - 0 KW - GTPase-Activating Proteins KW - RNA, Messenger KW - Tumor Suppressor Proteins KW - Index Medicus KW - Animals KW - Cytoskeleton -- metabolism KW - Transcription, Genetic -- genetics KW - Mice KW - RNA, Messenger -- genetics KW - Fibroblasts KW - Gene Deletion KW - Mice, Knockout KW - RNA, Messenger -- metabolism KW - Cells, Cultured KW - Heterozygote KW - Cytoskeleton -- pathology KW - Mutation -- genetics KW - Cytoskeleton -- genetics KW - Gene Expression Regulation, Developmental KW - Tumor Suppressor Proteins -- deficiency KW - GTPase-Activating Proteins -- genetics KW - GTPase-Activating Proteins -- metabolism KW - Embryo, Mammalian -- embryology KW - GTPase-Activating Proteins -- deficiency KW - Tumor Suppressor Proteins -- metabolism KW - Tumor Suppressor Proteins -- genetics KW - Embryo, Mammalian -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67426838?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+letters&rft.atitle=DLC-1%2C+a+Rho+GTPase-activating+protein+with+tumor+suppressor+function%2C+is+essential+for+embryonic+development.&rft.au=Durkin%2C+Marian+E%3BAvner%2C+Miriam+R%3BHuh%2C+Chang-Goo%3BYuan%2C+Bao-Zhu%3BThorgeirsson%2C+Snorri+S%3BPopescu%2C+Nicholas+C&rft.aulast=Durkin&rft.aufirst=Marian&rft.date=2005-02-14&rft.volume=579&rft.issue=5&rft.spage=1191&rft.isbn=&rft.btitle=&rft.title=FEBS+letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-02-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Psychopathology associated with drinking and alcohol use disorders in the college and general adult populations. AN - 67370889; 15664715 AB - This paper examines the associations between past-year drinking status and the prevalence of 15 different past-year anxiety, mood and personality disorders, using a large (n = 43,093) nationally representative sample of the U.S. population. The prevalence of these disorders and their associations with drinking are compared for college students 18-29 years of age, other youth 18-29 years of age, and adults 30 years of age and older. After adjusting for sociodemographic characteristics and past-year tobacco and illicit drug use, only drinkers with alcohol dependence experienced an excess risk of a mood or anxiety disorder among college students 18-29 years of age, OR = 2.4. In contrast, the excess risk of any mood or anxiety disorder associated with drinking status among non-college youth varied from an OR of 1.8 for non-binge drinkers to 4.7 for drinkers with alcohol dependence. Among persons 30 years of age and older, the degree of excess risk was slightly lower but still higher than those for college students, OR = 1.5-3.8. Similarly, the excess odds of any personality disorder associated with drinking varied from 1.6 to 5.0 for the younger, non-college group and from 1.5 to 3.8 for the older adults, with no significant effect observed among college students. Factors that may help explain the weaker association of psychopathology and drinking in the college population include selectivity and greater availability of social and treatment resources that serve as alternatives to self-medicating the symptoms of psychological distress with alcohol. JF - Drug and alcohol dependence AU - Dawson, Deborah A AU - Grant, Bridget F AU - Stinson, Frederick S AU - Chou, Patricia S AD - U.S. Department of Health and Human Services, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, NIAAA/DBE, Bethesda, MD 20892-7003, USA. ddawson@wqillco.niaaa.nih.gov Y1 - 2005/02/14/ PY - 2005 DA - 2005 Feb 14 SP - 139 EP - 150 VL - 77 IS - 2 SN - 0376-8716, 0376-8716 KW - Index Medicus KW - Humans KW - Adult KW - Psychopathology KW - Adolescent KW - Male KW - Female KW - Prevalence KW - Students -- psychology KW - Alcoholism -- epidemiology KW - Mental Disorders -- epidemiology KW - Alcohol Drinking -- psychology KW - Mental Disorders -- psychology KW - Mental Disorders -- etiology KW - Universities KW - Alcohol Drinking -- epidemiology KW - Alcoholism -- psychology KW - Alcoholism -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67370889?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+and+alcohol+dependence&rft.atitle=Psychopathology+associated+with+drinking+and+alcohol+use+disorders+in+the+college+and+general+adult+populations.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BStinson%2C+Frederick+S%3BChou%2C+Patricia+S&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-02-14&rft.volume=77&rft.issue=2&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=Drug+and+alcohol+dependence&rft.issn=03768716&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-01-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - CYP1A1 and CYP1B1 genotypes, haplotypes, and TCDD-induced gene expression in subjects from Seveso, Italy. AN - 67323316; 15596250 AB - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is highly toxic in experimental animals, and is known to induce cytochrome P450 (CYP) gene expression. We investigated the effect of CYP1A1 and CYP1B1 variant genotypes and haplotypes on CYP1A1 and CYP1B1 mRNA expression and ethoxyresorufin-O-deethylase (EROD) activity in lymphocytes from 121 subjects from the Seveso population, Italy, accidentally exposed to TCDD in 1976. The 3'UTR 3801T>C and I462V variants of CYP1A1 were present in 16% and 6% of the subjects, respectively. The frequency of CYP1B1 variants was 85.2% for L432V, 49.6% for R48G and A119S, and 28.7% for N453S. There was complete linkage disequilibrium (LD) among the CYP1B1 variant loci (D'=-1) and high LD among the CYP1A1 loci (D'=0.86). Gene expression measured by RT-PCR did not vary by CYP1B1 genotype in uncultured lymphocytes. However, when lymphocytes were treated in vitro with 10 nM TCDD, CYP1B1 and CYP1A1 mRNA expression was strongly induced and modified by CYP variant alleles. Specifically, the CYP1B1*3 haplotype (L432V) was associated with increased CYP1B1 mRNA expression (P=0.03), following an additive model; the CYP1A1 I462V polymorphism was positively, although not significantly, associated with CYP1A1 expression. The CYP1B1*3 variant may have affected CYP1B1 expression in subjects highly and acutely exposed to dioxin at the time of the accident. Although based on small number of subjects, a slight increase in eczema (P=0.05, n=8) and urticaria (P=0.02, n=2) was observed 20 years after the accident in subjects carrying the CYP1B1*3 allele. Genetic variation in cytochrome P450 induction may identify subjects with variable responsiveness to TCDD and potentially increased risk of disease. JF - Toxicology AU - Landi, Maria Teresa AU - Bergen, Andrew W AU - Baccarelli, Andrea AU - Patterson, Donald G AU - Grassman, Jean AU - Ter-Minassian, Monica AU - Mocarelli, Paolo AU - Caporaso, Neil AU - Masten, Scott A AU - Pesatori, Angela C AU - Pittman, Gary S AU - Bell, Douglas A AD - Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, 6120 Executive Blvd., EPS, Bethesda, MD 20892-7236, USA. landim@mail.nih.gov Y1 - 2005/02/14/ PY - 2005 DA - 2005 Feb 14 SP - 191 EP - 202 VL - 207 IS - 2 SN - 0300-483X, 0300-483X KW - Environmental Pollutants KW - 0 KW - Polychlorinated Dibenzodioxins KW - RNA KW - 63231-63-0 KW - Aryl Hydrocarbon Hydroxylases KW - EC 1.14.14.1 KW - CYP1B1 protein, human KW - Cytochrome P-450 CYP1A1 KW - Cytochrome P-450 CYP1B1 KW - Index Medicus KW - Genotype KW - Haplotypes KW - Enzyme Induction -- drug effects KW - Cells, Cultured KW - Humans KW - Male KW - Italy KW - Female KW - RNA -- biosynthesis KW - Leukocytes, Mononuclear -- enzymology KW - Environmental Pollutants -- pharmacology KW - Cytochrome P-450 CYP1A1 -- genetics KW - Environmental Pollutants -- toxicity KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Polychlorinated Dibenzodioxins -- pharmacology KW - Polychlorinated Dibenzodioxins -- toxicity KW - Aryl Hydrocarbon Hydroxylases -- genetics KW - Leukocytes, Mononuclear -- drug effects KW - Cytochrome P-450 CYP1A1 -- biosynthesis KW - Aryl Hydrocarbon Hydroxylases -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67323316?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology&rft.atitle=CYP1A1+and+CYP1B1+genotypes%2C+haplotypes%2C+and+TCDD-induced+gene+expression+in+subjects+from+Seveso%2C+Italy.&rft.au=Landi%2C+Maria+Teresa%3BBergen%2C+Andrew+W%3BBaccarelli%2C+Andrea%3BPatterson%2C+Donald+G%3BGrassman%2C+Jean%3BTer-Minassian%2C+Monica%3BMocarelli%2C+Paolo%3BCaporaso%2C+Neil%3BMasten%2C+Scott+A%3BPesatori%2C+Angela+C%3BPittman%2C+Gary+S%3BBell%2C+Douglas+A&rft.aulast=Landi&rft.aufirst=Maria&rft.date=2005-02-14&rft.volume=207&rft.issue=2&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Toxicology&rft.issn=0300483X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-21 N1 - Date created - 2004-12-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A region in the seven-transmembrane domain of the human Ca2+ receptor critical for response to Ca2+. AN - 67407185; 15591042 AB - Of 12 naturally occurring, activating mutations in the seven-transmembrane (7TM) domain of the human Ca2+ receptor (CaR) identified previously in subjects with autosomal dominant hypocalcemia (ADH), five appear at the junction of TM helices 6 and 7 between residue Ile819 and Glu837. After identifying a sixth activating mutation in this region, V836L, in an ADH patient, we studied the remaining residues in this region to determine whether they are potential sites for activating mutations. Alanine-scanning mutagenesis revealed five additional residues in this region that when substituted by alanine led to CaR activation. We also found that, whereas E837A did not activate the receptor, E837D and E837K mutations did. Thus, region Ile819-Glu837 of the 7TM domain represents a "hot spot" for naturally occurring, activating mutations of the receptor, and most of the residues in this region apparently maintain the 7TM domain in its inactive configuration. Unique among the residues in this region, Pro823, which is highly conserved in family 3 of the G protein-coupled receptors, when mutated to either alanine or glycine, despite good expression severely impaired CaR activation by Ca2+. Both the P823A mutation and NPS 2143, a negative allosteric modulator that acts on the 7TM through a critical interaction with Glu837, blocked activation of the CaR by various ADH mutations. These results suggest that the 7TM domain region Ile819-Glu837 plays a key role in CaR activation by Ca2+. The implications of our finding that NPS 2143 corrects the molecular defect of ADH mutations for treatment of this disease are also discussed. JF - The Journal of biological chemistry AU - Hu, Jianxin AU - McLarnon, Stuart J AU - Mora, Stefano AU - Jiang, Jiankang AU - Thomas, Craig AU - Jacobson, Kenneth A AU - Spiegel, Allen M AD - Molecular Pathophysiology Section, NIDCD, National Institutes of Health, Bethesda, Maryland 20892, USA. jianxinh@intra.niddk.nih.gov Y1 - 2005/02/11/ PY - 2005 DA - 2005 Feb 11 SP - 5113 EP - 5120 VL - 280 IS - 6 SN - 0021-9258, 0021-9258 KW - Ions KW - 0 KW - Phosphatidylinositols KW - Receptors, Calcium-Sensing KW - Isoleucine KW - 04Y7590D77 KW - Glutamine KW - 0RH81L854J KW - Proline KW - 9DLQ4CIU6V KW - Alanine KW - OF5P57N2ZX KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Phosphatidylinositols -- chemistry KW - Proline -- chemistry KW - Immunoblotting KW - Protein Structure, Secondary KW - Dose-Response Relationship, Drug KW - Electrophoresis, Polyacrylamide Gel KW - Humans KW - Alanine -- chemistry KW - Protein Binding KW - Hydrolysis KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Transfection KW - Molecular Sequence Data KW - Glutamine -- chemistry KW - Cell Membrane -- metabolism KW - Protein Structure, Tertiary KW - Allosteric Site KW - Mutation KW - Cell Line KW - Isoleucine -- chemistry KW - Calcium -- metabolism KW - Receptors, Calcium-Sensing -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67407185?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=A+region+in+the+seven-transmembrane+domain+of+the+human+Ca2%2B+receptor+critical+for+response+to+Ca2%2B.&rft.au=Hu%2C+Jianxin%3BMcLarnon%2C+Stuart+J%3BMora%2C+Stefano%3BJiang%2C+Jiankang%3BThomas%2C+Craig%3BJacobson%2C+Kenneth+A%3BSpiegel%2C+Allen+M&rft.aulast=Hu&rft.aufirst=Jianxin&rft.date=2005-02-11&rft.volume=280&rft.issue=6&rft.spage=5113&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-02-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Epigenetic silencing of the human nucleotide excision repair gene, hHR23B, in interleukin-6-responsive multiple myeloma KAS-6/1 cells. AN - 67406179; 15550378 AB - During tumorigenesis, selective proliferative advantage in certain cell subsets is associated with accumulation of multiple genetic alterations. For instance, multiple myeloma is characterized by frequent karyotypic instability at the earliest stage, progressing to extreme genetic abnormalities as the disease progresses. These successive genetic alterations can be attributed, in part, to defects in DNA repair pathways, perhaps based on epigenetic gene silencing of proteins involved in DNA damage repair. Here we report epigenetic hypermethylation of the hHR23B gene, a key component of the nucleotide excision repair in response to DNA damage, in interleukin-6 (IL-6)-responsive myeloma KAS-6/1 cells. This hypermethylation was significantly abated by Zebularine, a potent demethylating agent, with a consequent increase in the hHR23B mRNA level. Subsequent removal of this drug and supplementation with IL-6 in the culture medium re-established DNA hypermethylation of the hHR23B gene and silencing of mRNA expression levels. The inclination of DNA to be remethylated, at least within the hHR23B gene promoter region, reflects an epigenetic driving force by the cytogenetic/tumorigenic status of KAS-6/1 myeloma. The IL-6 response of KAS-6/1 myeloma also raises a question of whether the proneoplastic growth factor, such as IL-6, supports the epigenetic silencing of important DNA repair genes via promoter hypermethylation during the development of multiple myeloma. JF - The Journal of biological chemistry AU - Peng, Benjamin AU - Hodge, David R AU - Thomas, Suneetha Betsy AU - Cherry, James M AU - Munroe, David J AU - Pompeia, Celine AU - Xiao, Weihua AU - Farrar, William L AD - Cytokine Molecular Mechanisms Section, Laboratory of Molecular Immunoregulation, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA. Y1 - 2005/02/11/ PY - 2005 DA - 2005 Feb 11 SP - 4182 EP - 4187 VL - 280 IS - 6 SN - 0021-9258, 0021-9258 KW - Culture Media KW - 0 KW - DNA, Complementary KW - DNA-Binding Proteins KW - Interleukin-6 KW - Pyrimidine Nucleosides KW - RAD23B protein, human KW - RNA, Messenger KW - Sulfites KW - RAD23A protein, human KW - 156533-33-4 KW - Cytidine KW - 5CSZ8459RP KW - pyrimidin-2-one beta-ribofuranoside KW - 7A9Y5SX0GY KW - Cytosine KW - 8J337D1HZY KW - DNA KW - 9007-49-2 KW - DNA Repair Enzymes KW - EC 6.5.1.- KW - Index Medicus KW - Karyotyping KW - Ultraviolet Rays KW - DNA Damage KW - Gene Silencing KW - Humans KW - Pyrimidine Nucleosides -- pharmacology KW - Transcription, Genetic KW - Culture Media -- pharmacology KW - Gene Expression Regulation, Neoplastic KW - Promoter Regions, Genetic KW - DNA Methylation KW - Down-Regulation KW - Genes, Reporter KW - Molecular Sequence Data KW - Gene Expression Regulation KW - Plasmids -- metabolism KW - DNA Repair KW - Sulfites -- pharmacology KW - Cytosine -- chemistry KW - DNA -- metabolism KW - Cell Line, Tumor KW - Dose-Response Relationship, Radiation KW - Reverse Transcriptase Polymerase Chain Reaction KW - Cytidine -- analogs & derivatives KW - Base Sequence KW - RNA, Messenger -- metabolism KW - DNA, Complementary -- metabolism KW - CpG Islands KW - Kinetics KW - Interleukin-6 -- metabolism KW - Multiple Myeloma -- metabolism KW - DNA-Binding Proteins -- genetics KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67406179?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Epigenetic+silencing+of+the+human+nucleotide+excision+repair+gene%2C+hHR23B%2C+in+interleukin-6-responsive+multiple+myeloma+KAS-6%2F1+cells.&rft.au=Peng%2C+Benjamin%3BHodge%2C+David+R%3BThomas%2C+Suneetha+Betsy%3BCherry%2C+James+M%3BMunroe%2C+David+J%3BPompeia%2C+Celine%3BXiao%2C+Weihua%3BFarrar%2C+William+L&rft.aulast=Peng&rft.aufirst=Benjamin&rft.date=2005-02-11&rft.volume=280&rft.issue=6&rft.spage=4182&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-05 N1 - Date created - 2005-02-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Novel Heterocyclic Trans Olefin Analogues of N-{4-[4-(2,3-Dichlorophenyl) piperazin-1-yl]butyl}arylcarboxamides as Selective Probes with High Affinity for the Dopamine D3 Receptor AN - 19382094; 7149639 AB - Dopamine D3 receptor subtypes have been hypothesized to play a pivotal role in modulating the reinforcing and drug-seeking effects induced by cocaine. However, definitive pharmacological investigations have been hampered by the lack of highly D3 receptor selective compounds that can be used in vivo. To address this problem, the potent and D3-receptor-selective antagonist NGB 2904 (1, 9H-fluorene-2-carboxylic acid {4-[(2,3-dichlorophenyl)-piperazin-1-yl]-butyl}-amide, K sub(i) (hD3) = 2.0 nM, K sub(i) (hD2 sub(L)) = 112 nM, D2/D3 selectivity ratio of 56) was chosen as a lead structure for chemical modification in an attempt to reduce its high lipophilicity (c log D = 6.94) while optimizing D3 receptor binding affinity and D2/D3 selectivity. A series of >30 novel analogues were synthesized, and their binding affinities were evaluated in competition binding assays in HEK 293 cells transfected with either D2 sub(L), D3, or D4 human dopamine receptors using the high affinity, selective D2-like receptor antagonist super(125)I-IABN. Structural diversity in the aryl amide end of the molecule was found to have a major influence on (sub)nanomolar D3 receptor affinity and D2/D3 selectivity, which was optimized using a more rigid trans-butcnyl linker between the aryl amide and the piperazine. Several analogues demonstrated superior D3 receptor binding affinities and selectivities as compared to the parent ligand. Compound 29 (N-{4-[4-(2,3-dichloropheny the most promising pharmacological profile (K sub(i) (hD3) = 0.7 nM, K sub(i) (hD2 sub(L)) = 93.3 nM, D2/D3 selectivity ratio of 133). In addition, this ligand inhibited quinpirole stimulation of mitogenesis at human dopamine D3 receptors transfected into Chinese hamster ovary (CHO) cells, with an EC sub(50) value of 3.0 nM. Compound 29 was a nearly 5 times more potent antagonist at the D3 receptor than 1 (EC sub(50) = 14.4 nM). Moreover, a decrease in c log D value of similar to 2 orders of magnitude was determined for this novel D3-receptor-preferring ligand, compared to 1. In summary, chemical modification of 1 has resulted in compounds with high affinity and selectivity for D3 receptors. The most promising candidate, compound 29, is currently being evaluated in animal models of cocaine abuse and will provide an important tool with which to elucidate the role of D3 receptors in drug reinforcement in vivo. JF - Journal of Medicinal Chemistry AU - Grundt, P AU - Carlson, EE AU - Cao, J AU - Bennett, C J AU - McElveen, E AU - Taylor, M AU - Luedtke, R R AU - Newman, AH AD - Medicinal Chemistry Section, National Institute on Drug Abuse-Intramural Research Program, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland 21224, USA Y1 - 2005/02/10/ PY - 2005 DA - 2005 Feb 10 SP - 839 EP - 848 VL - 48 IS - 3 SN - 0022-2623, 0022-2623 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Dopamine D3 receptors KW - Probes KW - piperazine KW - Animal models KW - quinpirole KW - Drug development KW - Drug abuse KW - Dopamine D4 receptors KW - Cocaine KW - amides KW - Chemical modification KW - N3 11008:Neurochemistry KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19382094?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Novel+Heterocyclic+Trans+Olefin+Analogues+of+N-%7B4-%5B4-%282%2C3-Dichlorophenyl%29+piperazin-1-yl%5Dbutyl%7Darylcarboxamides+as+Selective+Probes+with+High+Affinity+for+the+Dopamine+D3+Receptor&rft.au=Grundt%2C+P%3BCarlson%2C+EE%3BCao%2C+J%3BBennett%2C+C+J%3BMcElveen%2C+E%3BTaylor%2C+M%3BLuedtke%2C+R+R%3BNewman%2C+AH&rft.aulast=Grundt&rft.aufirst=P&rft.date=2005-02-10&rft.volume=48&rft.issue=3&rft.spage=839&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm049465g LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Dopamine D3 receptors; Chemical modification; amides; Cocaine; Drug abuse; Drug development; Dopamine D4 receptors; Animal models; quinpirole; piperazine; Probes DO - http://dx.doi.org/10.1021/jm049465g ER - TY - JOUR T1 - Design and Synthesis of Conformationally Constrained Grb2 SH2 Domain Binding Peptides Employing alpha -Methylphenylalanyl Based Phosphotyrosyl Mimetics AN - 19380673; 7149636 AB - Previous work has shown that incorporation of either 1-aminocyclohexanecarboxylic acid (Ac sub(6)c) or alpha -methyl-p-phosphonophenylalanine (( alpha -Me)Ppp) in the phosphotyrosyl (pTyr) C-proximal position (pY + 1 residue) of Grb2 SH2 domain binding peptides confers high affinity. The tetralin-based (S)-2-amino-6-phosphonotetralin-2-carboxylic acid (Atc(6-PO sub(3)H sub(2))) simultaneously presents structural features of both ( alpha -Me)Ppp and Ac sub(6)c residues. The current study compares the affinity of this tetralin hybrid Atc(6-PO sub(3)H sub(2)) versus Ac sub(6)c and ( alpha -Me)Ppp residues when incorporated into the pY + 1 position of a high-affinity Grb2 SH2 domain binding tripeptide platform. The highest binding affinity (K sub(D) = 14.8 nM) was exhibited by the ( alpha -Me)Ppp-containing parent, with the corresponding Ac sub(6)c-containing peptide being nearly 2-fold less potent (K sub(D) = 23.8 nM). The lower K sub(D) value was attributable primarily to a 50% increase in off-rate. Replacement of the Ac sub(6)c residue with the tetralin-based hybrid resulted in a further 4-fold decrease in binding affhity (K sub(D) = 97.8 nM), which was the result of a further 6-fold increase in off-rate, offset by an approximate 45% increase in on-rate. Therefore, by incorporation of the key structural components found in ( alpha -Me)Ppp into the Ac sub(6)c residue, the tetralin hybrid does enhance binding on-rate. However, net binding affinity is decreased due to an associated increase in binding off-rate. Alternatively, global conformational constraint of an ( alpha -Me)Ppp-containing peptide by beta -macrocyclization did result in pronounced elevation of binding affinity, which was achieved primarily through a decrease in the binding off-rate. Mathematical fitting using a simple model that assumed a single binding site yielded an effective K sub(D) of 2.28 nM. However this did not closely approximate the data obtained. Rather, use of a complex model that assumed two binding sites resulted in a very close fit of data and provided K sub(D) values of 97 pM and 72 nM for the separate sites, respectively. Therefore, although local conformational constraint in the pY + 1 residue proved to be deleterious, global conformational constraint through beta -macrocyclization achieved higher affinity. JF - Journal of Medicinal Chemistry AU - Oishi, S AU - Karki, R G AU - Kang, S-U AU - Wang, X AU - Worthy, K M AU - Bindu, L K AU - Nicklaus, M C AU - Fisher, R J AU - Burke, TR Jr AD - Laboratory of Medicinal Chemistry, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA Y1 - 2005/02/10/ PY - 2005 DA - 2005 Feb 10 SP - 764 EP - 772 VL - 48 IS - 3 SN - 0022-2623, 0022-2623 KW - Biotechnology and Bioengineering Abstracts KW - Grb2 protein KW - Mathematical models KW - Hybrids KW - tetralin KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19380673?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medicinal+Chemistry&rft.atitle=Design+and+Synthesis+of+Conformationally+Constrained+Grb2+SH2+Domain+Binding+Peptides+Employing+alpha+-Methylphenylalanyl+Based+Phosphotyrosyl+Mimetics&rft.au=Oishi%2C+S%3BKarki%2C+R+G%3BKang%2C+S-U%3BWang%2C+X%3BWorthy%2C+K+M%3BBindu%2C+L+K%3BNicklaus%2C+M+C%3BFisher%2C+R+J%3BBurke%2C+TR+Jr&rft.aulast=Oishi&rft.aufirst=S&rft.date=2005-02-10&rft.volume=48&rft.issue=3&rft.spage=764&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medicinal+Chemistry&rft.issn=00222623&rft_id=info:doi/10.1021%2Fjm0492709 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Hybrids; Grb2 protein; tetralin; Mathematical models DO - http://dx.doi.org/10.1021/jm0492709 ER - TY - JOUR T1 - Effects of cholinergic deafferentation of the rhinal cortex on visual recognition memory in monkeys. AN - 67419233; 15684066 AB - Excitotoxic lesion studies have confirmed that the rhinal cortex is essential for visual recognition ability in monkeys. To evaluate the mnemonic role of cholinergic inputs to this cortical region, we compared the visual recognition performance of monkeys given rhinal cortex infusions of a selective cholinergic immunotoxin, ME20.4-SAP, with the performance of monkeys given control infusions into this same tissue. The immunotoxin, which leads to selective cholinergic deafferentation of the infused cortex, yielded recognition deficits of the same magnitude as those produced by excitotoxic lesions of this region, providing the most direct demonstration to date that cholinergic activation of the rhinal cortex is essential for storing the representations of new visual stimuli and thereby enabling their later recognition. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Turchi, Janita AU - Saunders, Richard C AU - Mishkin, Mortimer AD - Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health, Building 49, Room 1B80, Bethesda, MD 20892, USA. turchij@mail.nih.gov Y1 - 2005/02/08/ PY - 2005 DA - 2005 Feb 08 SP - 2158 EP - 2161 VL - 102 IS - 6 SN - 0027-8424, 0027-8424 KW - Acetylcholine KW - N9YNS0M02X KW - Index Medicus KW - Animals KW - Humans KW - Macaca mulatta KW - Behavior -- physiology KW - Male KW - Recognition (Psychology) -- physiology KW - Entorhinal Cortex -- pathology KW - Acetylcholine -- metabolism KW - Entorhinal Cortex -- physiology KW - Entorhinal Cortex -- anatomy & histology KW - Afferent Pathways -- anatomy & histology KW - Memory -- physiology KW - Pattern Recognition, Visual -- physiology KW - Afferent Pathways -- metabolism KW - Afferent Pathways -- surgery UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67419233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Effects+of+cholinergic+deafferentation+of+the+rhinal+cortex+on+visual+recognition+memory+in+monkeys.&rft.au=Turchi%2C+Janita%3BSaunders%2C+Richard+C%3BMishkin%2C+Mortimer&rft.aulast=Turchi&rft.aufirst=Janita&rft.date=2005-02-08&rft.volume=102&rft.issue=6&rft.spage=2158&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-04-22 N1 - Date created - 2005-02-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Neuron. 2003 Jun 19;38(6):987-96 [12818183] Psychopharmacology (Berl). 1997 Nov;134(1):95-106 [9399372] Neurol Res. 1979;1(2):203-9 [95356] J Comp Neurol. 1983 Feb 20;214(2):170-97 [6841683] Eur J Neurosci. 2001 Mar;13(6):1228-38 [11285020] Neuroreport. 2001 Jul 3;12(9):1913-7 [11435922] Cereb Cortex. 2002 Jul;12(7):729-36 [12050084] Brain Res Bull. 1985 Sep;15(3):307-14 [2413969] Behav Neural Biol. 1986 Jan;45(1):81-7 [3954717] Brain Res. 1986 Mar 5;367(1-2):301-8 [3516304] J Histochem Cytochem. 1986 Nov;34(11):1431-8 [2430009] Psychopharmacology (Berl). 1987;92(3):292-300 [3114781] Brain. 1988 Jun;111 ( Pt 3):695-718 [3382917] Brain Res. 1988 Oct 25;463(1):133-9 [3196902] Behav Neural Biol. 1991 Jan;55(1):61-7 [1996948] Exp Brain Res. 1991;86(1):18-26 [1756788] Brain Res. 1991 Oct 18;562(1):149-53 [1666014] Trends Neurosci. 1991 Nov;14(11):494-501 [1726766] J Neurosci. 1993 Jun;13(6):2430-51 [8501516] J Comp Neurol. 1999 May 24;408(1):11-22 [10331577] J Neurosci. 1993 Dec;13(12):5418-32 [8254384] J Neurosci. 1994 Jan;14(1):167-86 [8283232] J Neurosci. 1994 Mar;14(3 Pt 1):1271-89 [8120624] Brain Res. 1994 Nov 14;663(2):277-86 [7874512] Pharmacol Biochem Behav. 1996 Feb;53(2):277-83 [8808132] Neuroreport. 1996 Sep 2;7(13):2231-5 [8930995] Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12667-9 [9356507] Neurosci Lett. 2004 Feb 19;356(3):191-4 [15036627] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cerebrovascular perfusion in marijuana users during a month of monitored abstinence. AN - 67414425; 15699380 AB - To determine possible effects of prolonged marijuana use on the cerebrovascular system during a month of monitored abstinence and to assess how the intensity of current use might have influenced cerebrovascular perfusion in these marijuana users. The authors recorded blood flow velocity in the anterior and middle cerebral arteries using transcranial Doppler sonography in three groups of marijuana users who differed in the intensity of recent use (light: n = 11; moderate: n = 23; and heavy: n = 20) and in control subjects (n = 18) to assess the nature and duration of any potential abnormalities. Blood flow velocity was recorded within 3 days of admission and 28 to 30 days of monitored abstinence on an inpatient research unit in order to evaluate subacute effects of the drug and any abstinence-generated changes. Pulsatility index, a measure of cerebrovascular resistance, and systolic velocity were significantly increased in the marijuana users vs control subjects. These increases persisted in the heavy marijuana users after a month of monitored abstinence. Chronic marijuana use is associated with increased cerebrovascular resistance through changes mediated, in part, in blood vessels or in the brain parenchyma. These findings might provide a partial explanation for the cognitive deficits observed in a similar group of marijuana users. JF - Neurology AU - Herning, Ronald I AU - Better, Warren E AU - Tate, Kimberly AU - Cadet, Jean L AD - Molecular Neuropsychiatry Branch, National Institute on Drug Abuse, National Institutes of Health, PO Box 5180, Baltimore, MD 21224, USA. rherning@intra.nida.nih.gov Y1 - 2005/02/08/ PY - 2005 DA - 2005 Feb 08 SP - 488 EP - 493 VL - 64 IS - 3 KW - Abridged Index Medicus KW - Index Medicus KW - Hemodynamics -- drug effects KW - Severity of Illness Index KW - Tobacco Use Disorder -- physiopathology KW - Blood Flow Velocity KW - Humans KW - Ultrasonography, Doppler, Transcranial KW - Vascular Resistance -- drug effects KW - Smoking -- physiopathology KW - Antisocial Personality Disorder -- complications KW - Adult KW - Alcohol Drinking -- physiopathology KW - Inpatients KW - Adolescent KW - Tobacco Use Disorder -- complications KW - Time Factors KW - Female KW - Male KW - Antisocial Personality Disorder -- physiopathology KW - Substance Withdrawal Syndrome -- physiopathology KW - Marijuana Abuse -- complications KW - Cerebrovascular Circulation -- drug effects KW - Cerebral Arteries -- physiopathology KW - Cannabis -- adverse effects KW - Marijuana Smoking -- physiopathology KW - Marijuana Abuse -- physiopathology KW - Cerebral Arteries -- diagnostic imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67414425?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurology&rft.atitle=Cerebrovascular+perfusion+in+marijuana+users+during+a+month+of+monitored+abstinence.&rft.au=Herning%2C+Ronald+I%3BBetter%2C+Warren+E%3BTate%2C+Kimberly%3BCadet%2C+Jean+L&rft.aulast=Herning&rft.aufirst=Ronald&rft.date=2005-02-08&rft.volume=64&rft.issue=3&rft.spage=488&rft.isbn=&rft.btitle=&rft.title=Neurology&rft.issn=1526-632X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-02-08 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Neurology. 2005 Oct 11;65(7):1145; author reply 1145 [16217086] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In the quest for stable rescuing mutants of p53: computational mutagenesis of flexible loop L1. AN - 67394279; 15683227 AB - p53 is a protein with marginal stability. Its transcriptional functions are often inactivated by single missense mutations, shown to be associated with half of all human cancers. Here, we aim to design stable functional p53 mutants. We target loop L1, one of the most mobile structural motifs in the p53 core domain (p53C). Specifically, we selected Ser116 in the middle of loop L1 and mutated it to 14 other amino acids. All resulting mutants were subjected to molecular dynamics simulations, revealing a wide spectrum of stabilities. Among these, mutant S116M displayed a remarkable stability, with a structural deviation comparable to that of the experimental quadruple mutant M133L/V203A/N239Y/N268D that is thermodynamically more stable than that of the wild type by 2.6 kcal/mol. Structural analysis showed that the high stability of the S116M mutant was indeed due to the preservation of the p53C loop L1 conformation and the reduction of mobility in that region. The differential stabilities conferred by the single mutations are rationalized based on the geometries and chemical properties of the side chains introduced into this site. Linearity (i.e., nonbranched), moderate size, and balanced hydrophobic and hydrophilic properties of the side chain are crucial to the stabilizing effect of the residue substitutions. JF - Biochemistry AU - Pan, Yongping AU - Ma, Buyong AU - Venkataraghavan, R Babu AU - Levine, Arnold J AU - Nussinov, Ruth AD - Basic Research Program, SAIC-Frederick, Incorporated, Laboratory of Experimental and Computational Biology, NCI-Frederick, Frederick, Maryland 21702, USA. Y1 - 2005/02/08/ PY - 2005 DA - 2005 Feb 08 SP - 1423 EP - 1432 VL - 44 IS - 5 SN - 0006-2960, 0006-2960 KW - Neoplasm Proteins KW - 0 KW - Tumor Suppressor Protein p53 KW - Serine KW - 452VLY9402 KW - Proline KW - 9DLQ4CIU6V KW - Methionine KW - AE28F7PNPL KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Proline -- genetics KW - Methionine -- genetics KW - Amino Acid Substitution -- genetics KW - Humans KW - Cysteine -- genetics KW - Computational Biology -- methods KW - Protein Structure, Secondary -- genetics KW - Hydrogen Bonding KW - Serine -- genetics KW - Protein Conformation KW - Mutagenesis, Site-Directed KW - Computer Simulation KW - Thermodynamics KW - Models, Molecular KW - Neoplasm Proteins -- genetics KW - Tumor Suppressor Protein p53 -- chemistry KW - Neoplasm Proteins -- chemistry KW - Tumor Suppressor Protein p53 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67394279?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=In+the+quest+for+stable+rescuing+mutants+of+p53%3A+computational+mutagenesis+of+flexible+loop+L1.&rft.au=Pan%2C+Yongping%3BMa%2C+Buyong%3BVenkataraghavan%2C+R+Babu%3BLevine%2C+Arnold+J%3BNussinov%2C+Ruth&rft.aulast=Pan&rft.aufirst=Yongping&rft.date=2005-02-08&rft.volume=44&rft.issue=5&rft.spage=1423&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=00062960&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-02-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Portraits of a Y-family DNA polymerase. AN - 67392539; 15680965 AB - Members of the Y-family of DNA polymerases catalyze template-dependent DNA synthesis but share no sequence homology with other known DNA polymerases. Y-family polymerases exhibit high error rates and low processivity when copying normal DNA but are able to synthesize DNA opposite damaged templates. In the past three years, much has been learned about this family of polymerases including determination of more than a dozen crystal structures with various substrates. In this short review, I will summarize the biochemical properties and structural features of Y-family DNA polymerases. JF - FEBS letters AU - Yang, Wei AD - Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Wei.Yang@nih.gov Y1 - 2005/02/07/ PY - 2005 DA - 2005 Feb 07 SP - 868 EP - 872 VL - 579 IS - 4 SN - 0014-5793, 0014-5793 KW - Archaeal Proteins KW - 0 KW - Pyrimidine Dimers KW - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide KW - 55097-80-8 KW - DNA KW - 9007-49-2 KW - DNA-Directed DNA Polymerase KW - EC 2.7.7.7 KW - Index Medicus KW - Molecular Structure KW - Base Pairing -- drug effects KW - Pyrimidine Dimers -- metabolism KW - DNA Damage KW - DNA -- metabolism KW - Archaea -- enzymology KW - Crystallography KW - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide -- pharmacology KW - Protein Conformation KW - DNA -- drug effects KW - Binding Sites KW - DNA-Directed DNA Polymerase -- physiology KW - Archaeal Proteins -- physiology KW - Archaeal Proteins -- chemistry KW - DNA-Directed DNA Polymerase -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67392539?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+letters&rft.atitle=Portraits+of+a+Y-family+DNA+polymerase.&rft.au=Yang%2C+Wei&rft.aulast=Yang&rft.aufirst=Wei&rft.date=2005-02-07&rft.volume=579&rft.issue=4&rft.spage=868&rft.isbn=&rft.btitle=&rft.title=FEBS+letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-03-10 N1 - Date created - 2005-01-31 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An integrated model of Plasmodium falciparum dynamics AN - 20931079; 8246764 AB - The within-host and between-host dynamics of malaria are linked in myriad ways, but most obviously by gametocytes, the parasite blood forms transmissible from human to mosquito. Gametocyte dynamics depend on those of non-transmissible blood forms, which stimulate immune responses, impeding transmission as well as within-host parasite densities. These dynamics can, in turn, influence antigenic diversity and recombination between genetically distinct parasites. Here, we embed a differential-equation model of parasite-immune system interactions within each of the individual humans represented in a discrete-event model of Plasmodium falciparum transmission, and examine the effects of human population turnover, parasite antigenic diversity, recombination, and gametocyte production on the dynamics of malaria. Our results indicate that the local persistence of P. falciparum increases with turnover in the human population and antigenic diversity in the parasite, particularly in combination, and that antigenic diversity arising from meiotic recombination in the parasite has complex differential effects on the persistence of founder and progeny genotypes. We also find that reductions in the duration of individual human infectivity to mosquitoes, even if universal, produce population-level effects only if near-absolute, and that, in competition, the persistence and prevalence of parasite genotypes with gametocyte production concordant with data exceed those of genotypes with higher gametocyte production. This new, integrated approach provides a framework for investigating relationships between pathogen dynamics within an individual host and pathogen dynamics within interacting host and vector populations. JF - Journal of Theoretical Biology AU - McKenzie, F E AU - Bossert, W H AD - Division of Engineering and Applied Sciences, Harvard University, 33 Oxford Street, Cambridge, MA 02138, USA, mckenzel@mail.nih.gov Y1 - 2005/02/07/ PY - 2005 DA - 2005 Feb 07 SP - 411 EP - 426 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl] VL - 232 IS - 3 SN - 0022-5193, 0022-5193 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Genetics Abstracts; Ecology Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Immunology Abstracts KW - Parasites KW - Human diseases KW - Malaria KW - Hosts KW - Genotypes KW - Models KW - Public health KW - Disease transmission KW - Recombination KW - Competition KW - Gametocytes KW - Vectors KW - Plasmodium falciparum KW - Pathogens KW - Blood KW - Infectivity KW - Meiosis KW - Progeny KW - K 03350:Immunology KW - G 07800:Plants and Algae KW - D 04030:Models, Methods, Remote Sensing KW - Q1 08484:Species interactions: parasites and diseases KW - F 06910:Microorganisms & Parasites KW - Q5 08524:Public health, medicines, dangerous organisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20931079?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Theoretical+Biology&rft.atitle=An+integrated+model+of+Plasmodium+falciparum+dynamics&rft.au=McKenzie%2C+F+E%3BBossert%2C+W+H&rft.aulast=McKenzie&rft.aufirst=F&rft.date=2005-02-07&rft.volume=232&rft.issue=3&rft.spage=411&rft.isbn=&rft.btitle=&rft.title=Journal+of+Theoretical+Biology&rft.issn=00225193&rft_id=info:doi/10.1016%2Fj.jtbi.2004.08.021 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Recombination; Parasites; Human diseases; Malaria; Pathogens; Genotypes; Hosts; Disease transmission; Public health; Gametocytes; Vectors; Models; Blood; Infectivity; Meiosis; Progeny; Competition; Plasmodium falciparum DO - http://dx.doi.org/10.1016/j.jtbi.2004.08.021 ER - TY - JOUR T1 - Transmitted mutational events induced in mouse germ cells following acrylamide or glycidamide exposure AN - 17815465; 6198011 AB - An increase in the germ line mutation rate in humans will result in an increase in the incidence of genetically determined diseases in subsequent generations. Thus, it is important to identify those agents that are mutagenic in mammalian germ cells. Acrylamide is water soluble, absorbed and distributed in the body, chemically reactive with nucleophilic sites, and there are known sources of human exposure. Here we review all seven published studies that assessed the effectiveness of acrylamide or its active metabolite, glycidamide, in inducing transmitted reciprocal translocations or gene mutations in the mouse. Major conclusions were (a) acrylamide is mutagenic in spermatozoa and spermatid stages of the male germ line; (b) in these spermatogenic stages acrylamide is mainly or exclusively a clastogen; (c) per unit dose, i.p. exposure is more effective than dermal exposure; and (d) per unit dose, glycidamide is more effective than acrylamide. Since stem cell spermatogonia persist and may accumulate mutations throughout the reproductive life of males, assessment of induced mutations in this germ cell stage is critical for the assessment of genetic risk associated with exposure to a mutagen. The two specific-locus mutation experiments which studied the stem cell spermatogonial stage yielded conflicting results. This discrepancy should be resolved. Finally, it is noted that no experiments have studied the mutagenic potential of acrylamide to increase the frequency of transmitted mutational events following exposure in the female germ line. JF - Mutation Research-Genetic Toxicology and Environmental Mutagenesis AU - Favor, J AU - Shelby, MD AD - NTP-CERHR, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA, favor@gsf.de Y1 - 2005/02/07/ PY - 2005 DA - 2005 Feb 07 SP - 21 EP - 30 VL - 580 IS - 1-2 SN - 1383-5718, 1383-5718 KW - glycidamide KW - mice KW - Genetics Abstracts; Toxicology Abstracts KW - Skin KW - Point mutation KW - Germ cells KW - Metabolites KW - Sperm KW - Mutation rates KW - Mutagenesis KW - Stem cells KW - Acrylamide KW - Spermatids KW - Reviews KW - Translocations KW - Spermatogonia KW - X 24222:Analytical procedures KW - G 07221:Specific chemicals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17815465?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.atitle=Transmitted+mutational+events+induced+in+mouse+germ+cells+following+acrylamide+or+glycidamide+exposure&rft.au=Favor%2C+J%3BShelby%2C+MD&rft.aulast=Favor&rft.aufirst=J&rft.date=2005-02-07&rft.volume=580&rft.issue=1-2&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Genetic+Toxicology+and+Environmental+Mutagenesis&rft.issn=13835718&rft_id=info:doi/10.1016%2Fj.mrgentox.2004.09.010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Acrylamide; Germ cells; Stem cells; Translocations; Sperm; Spermatogonia; Skin; Reviews; Metabolites; Point mutation; Spermatids; Mutation rates; Mutagenesis DO - http://dx.doi.org/10.1016/j.mrgentox.2004.09.010 ER -