TY - JOUR T1 - Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans. AN - 67892126; 15905333 AB - The pathways for differentiation of human CD4(+) T cells into functionally distinct subsets of memory cells in vivo are unknown. The identification of these subsets and pathways has clear implications for the design of vaccines and immune-targeted therapies. Here, we show that populations of apparently naive CD4(+) T cells express the chemokine receptors CXCR3 or CCR4 and demonstrate patterns of gene expression and functional responses characteristic of memory cells. The proliferation history and T cell receptor repertoire of these chemokine-receptor(+) cells suggest that they are very early memory CD4(+) T cells that have "rested down" before acquiring the phenotypes described for "central" or "effector" memory T cells. In addition, the chemokine-receptor(+) "naive" populations contain Th1 and Th2 cells, respectively, demonstrating that Th1/Th2 differentiation can occur very early in vivo in the absence of markers conventionally associated with memory cells. We localized ligands for CXCR3 and CCR4 to separate foci in T cell zones of tonsil, suggesting that the chemokine-receptor(+) subsets may be recruited and contribute to segregated, polarized microenvironments within lymphoid organs. Importantly, our data suggest that CD4(+) T cells do not differentiate according to a simple schema from naive --> CD45RO(+) noneffector/central memory --> effector/effector memory cells. Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Song, Kaimei AU - Rabin, Ronald L AU - Hill, Brenna J AU - De Rosa, Stephen C AU - Perfetto, Stephen P AU - Zhang, Hongwei H AU - Foley, John F AU - Reiner, Jeffrey S AU - Liu, Jie AU - Mattapallil, Joseph J AU - Douek, Daniel C AU - Roederer, Mario AU - Farber, Joshua M AD - Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/31/ PY - 2005 DA - 2005 May 31 SP - 7916 EP - 7921 VL - 102 IS - 22 SN - 0027-8424, 0027-8424 KW - CCR4 protein, human KW - 0 KW - CXCR3 protein, human KW - Ligands KW - Receptors, CCR4 KW - Receptors, CXCR3 KW - Receptors, Chemokine KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - In Situ Hybridization KW - Palatine Tonsil -- cytology KW - Cells, Cultured KW - Humans KW - Receptors, Chemokine -- metabolism KW - Flow Cytometry KW - Reverse Transcriptase Polymerase Chain Reaction KW - T-Lymphocyte Subsets -- cytology KW - CD4-Positive T-Lymphocytes -- cytology KW - Gene Expression -- immunology KW - CD4-Positive T-Lymphocytes -- metabolism KW - Immunologic Memory -- immunology KW - Cell Differentiation -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67892126?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Characterization+of+subsets+of+CD4%2B+memory+T+cells+reveals+early+branched+pathways+of+T+cell+differentiation+in+humans.&rft.au=Song%2C+Kaimei%3BRabin%2C+Ronald+L%3BHill%2C+Brenna+J%3BDe+Rosa%2C+Stephen+C%3BPerfetto%2C+Stephen+P%3BZhang%2C+Hongwei+H%3BFoley%2C+John+F%3BReiner%2C+Jeffrey+S%3BLiu%2C+Jie%3BMattapallil%2C+Joseph+J%3BDouek%2C+Daniel+C%3BRoederer%2C+Mario%3BFarber%2C+Joshua+M&rft.aulast=Song&rft.aufirst=Kaimei&rft.date=2005-05-31&rft.volume=102&rft.issue=22&rft.spage=7916&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-06-02 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nature. 1999 Oct 14;401(6754):708-12 [10537110] Nat Med. 2001 Feb;7(2):245-8 [11175858] Lancet. 2000 May 27;355(9218):1875-81 [10866444] J Leukoc Biol. 2000 Oct;68(4):568-74 [11037980] Immunity. 2001 Mar;14(3):205-15 [11290331] J Exp Med. 2001 Apr 16;193(8):987-93 [11304560] J Immunol Methods. 2002 Jan 1;259(1-2):55-64 [11730841] J Exp Med. 2002 Mar 18;195(6):789-94 [11901204] Nature. 2002 May 2;417(6884):95-8 [11986671] Nat Rev Immunol. 2002 Apr;2(4):251-62 [12001996] Eur J Immunol. 2002 May;32(5):1264-73 [11981813] Nat Rev Immunol. 2002 Dec;2(12):933-44 [12461566] J Immunol. 2003 Jan 1;170(1):28-32 [12496379] Nat Immunol. 2003 Mar;4(3):225-34 [12563257] Nat Immunol. 2003 Sep;4(9):835-42 [12942084] J Immunol. 2003 Sep 15;171(6):2812-24 [12960302] Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3874-9 [15001705] Annu Rev Immunol. 2004;22:745-63 [15032595] J Exp Med. 2004 Sep 20;200(6):725-35 [15381728] J Immunol. 1993 Feb 1;150(3):1105-21 [7678616] Cytometry. 1995 Oct 1;21(2):187-96 [8582239] J Immunol. 1999 Apr 1;162(7):3840-50 [10201901] Science. 1999 Apr 30;284(5415):819-22 [10221917] Scand J Immunol. 1999 Jun;49(6):649-54 [10354377] Int Immunol. 1999 Nov;11(11):1801-10 [10545484] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lymphocyte toxicity and T cell receptor excision circles in workers exposed to benzene. AN - 67902522; 15935806 AB - We have previously reported that benzene decreases peripheral white blood cell and platelet counts and specifically lowers subsets of several blood cell types, including CD4+-T cells, B cells, NK cells, and granulocytes. Diminished thymus function has been implicated as a mechanism for CD4+-T cell loss in other conditions such as AIDS by assays of T cell receptor excision circles (TRECs), a marker of naive T cells that have recently emigrated from the thymus. To evaluate alteration of thymic function as a mechanism for benzene's effects on CD4+-T cell counts, we measured total TREC levels in 45 benzene-exposed workers and 45 unexposed controls. There was no significant difference in TREC levels per 10(6) peripheral blood leukocytes in the benzene-exposed workers compared to the controls. Although our study does not rule out counterbalancing alterations of TREC levels in specific T cell subsets, benzene's lymphotoxicity does not appear to be mediated through diminished thymus function. JF - Chemico-biological interactions AU - Lan, Qing AU - Zhang, Luoping AU - Hakim, Fran AU - Shen, Min AU - Memon, Sarfraz AU - Li, Guilan AU - Vermeulen, Roel AU - Smith, Martyn T AU - Rappaport, Stephen M AU - Hayes, Richard AU - Linet, Martha AU - Yin, Songnian AU - Rothman, Nathaniel AU - Rabkin, Charles S AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, 6120 Executive Blvd., EPS 8109, Bethesda, MD 20892-7240, USA. qingl@mail.nih.gov Y1 - 2005/05/30/ PY - 2005 DA - 2005 May 30 SP - 111 EP - 115 VL - 153-154 SN - 0009-2797, 0009-2797 KW - Air Pollutants, Occupational KW - 0 KW - DNA, Circular KW - Receptors, Antigen, T-Cell KW - Benzene KW - J64922108F KW - Index Medicus KW - Leukocytes -- immunology KW - Humans KW - CD4-Positive T-Lymphocytes -- immunology KW - CD4-Positive T-Lymphocytes -- drug effects KW - Thymus Gland -- drug effects KW - Blood Cell Count KW - Environmental Monitoring KW - Thymus Gland -- immunology KW - Adult KW - Recombination, Genetic KW - Shoes KW - China KW - Female KW - Male KW - Occupational Exposure KW - Benzene -- analysis KW - Air Pollutants, Occupational -- analysis KW - DNA, Circular -- blood KW - Benzene -- toxicity KW - Receptors, Antigen, T-Cell -- immunology KW - Air Pollutants, Occupational -- toxicity KW - DNA, Circular -- analysis KW - Receptors, Antigen, T-Cell -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67902522?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chemico-biological+interactions&rft.atitle=Lymphocyte+toxicity+and+T+cell+receptor+excision+circles+in+workers+exposed+to+benzene.&rft.au=Lan%2C+Qing%3BZhang%2C+Luoping%3BHakim%2C+Fran%3BShen%2C+Min%3BMemon%2C+Sarfraz%3BLi%2C+Guilan%3BVermeulen%2C+Roel%3BSmith%2C+Martyn+T%3BRappaport%2C+Stephen+M%3BHayes%2C+Richard%3BLinet%2C+Martha%3BYin%2C+Songnian%3BRothman%2C+Nathaniel%3BRabkin%2C+Charles+S&rft.aulast=Lan&rft.aufirst=Qing&rft.date=2005-05-30&rft.volume=153-154&rft.issue=&rft.spage=111&rft.isbn=&rft.btitle=&rft.title=Chemico-biological+interactions&rft.issn=00092797&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-03 N1 - Date created - 2005-06-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in the pathogenesis of hepatocellular carcinoma. AN - 67871591; 15918183 AB - DNA mismatch repair (MMR) is an important mechanism for maintaining fidelity of genomic DNA. Abnormalities in one or more MMR genes are implicated in the development of many cancers. We investigated the role of expression of MMR genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in hepatocellular carcinogenesis. We evaluated the expression level of MMR genes in 33 hepatocellular carcinoma (HCC) cases using the multiplex reverse transcription (RT) PCR assays, as well as in 16 cases of normal adjacent hepatic tissues. beta-actin gene was used as an internal control and calibrator for quantification of gene expression. Out of the 33 studied cases, 25 were HCV positive and 30 (90.9%) showed reduced expression in one or more of the studied MMR genes. Reduced expression was found in hMSH2 (71.9%), hMLH1 (53.3%), GTBP (51.1%), hPMS2 (33.3%) and hPMS1 (6%). A significant correlation was found between reduced expression of hPMS2 (P = 0.0069) and GTBP (P = 0.0034), hPMS2 and non-cirrhosis (P = 0.0197), hMLH1 and high grade. On the other hand, 57.1%, 50%, 20%, 18.8%, and 6% of the normal tissues distant to tumors showed reduced expression of hMSH2, hMLH1, GTBP, hPMS2, and hPMS1 respectively. Multivariate analysis revealed a significant correlation between the expression level of hMSH2 (P = 0.008), hMLH1 (P = 0.001) and GTBP (P = 0.032) and HCC, between hPMS2, GTBP and HCV-associated HCC (P<0.001, 0.002). Reduced expression of MMR genes seems to play an important role in HCV-associated HCC. hPMS2 is likely involved at an early stage of hepatocarcinogenesis since it was detected in normal adjacent tissues. Reduced expression of hPMS2 provides a growth advantage and stimulates proliferation which encourages malignant transformation in non-cirrhotic HCV-infected patients via acquisition of more genetic damages. JF - World journal of gastroenterology AU - Zekri, Abdel-Rahman N AU - Sabry, Gelane M AU - Bahnassy, Abeer A AU - Shalaby, Kamal A AU - Abdel-Wahabh, Sabrin A AU - Zakaria, Serag AD - Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Kasr El-Aini St., Fom El-Khaig, Cairo 11796, Egypt. ncizakri@starnet.com.eg Y1 - 2005/05/28/ PY - 2005 DA - 2005 May 28 SP - 3020 EP - 3026 VL - 11 IS - 20 SN - 1007-9327, 1007-9327 KW - Index Medicus KW - Hepacivirus -- physiology KW - Humans KW - Middle Aged KW - Liver Cirrhosis -- genetics KW - Male KW - Female KW - DNA Repair -- genetics KW - Carcinoma, Hepatocellular -- virology KW - Liver Neoplasms -- pathology KW - Carcinoma, Hepatocellular -- genetics KW - Liver Neoplasms -- virology KW - Carcinoma, Hepatocellular -- pathology KW - Base Pair Mismatch -- genetics KW - Liver Neoplasms -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67871591?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=World+journal+of+gastroenterology&rft.atitle=Mismatch+repair+genes+%28hMLH1%2C+hPMS1%2C+hPMS2%2C+GTBP%2FhMSH6%2C+hMSH2%29+in+the+pathogenesis+of+hepatocellular+carcinoma.&rft.au=Zekri%2C+Abdel-Rahman+N%3BSabry%2C+Gelane+M%3BBahnassy%2C+Abeer+A%3BShalaby%2C+Kamal+A%3BAbdel-Wahabh%2C+Sabrin+A%3BZakaria%2C+Serag&rft.aulast=Zekri&rft.aufirst=Abdel-Rahman&rft.date=2005-05-28&rft.volume=11&rft.issue=20&rft.spage=3020&rft.isbn=&rft.btitle=&rft.title=Elementary+School+Journal&rft.issn=00135984&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Race against time. AN - 67869088; 15917781 JF - Nature AU - Fauci, Anthony S AD - National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-2520, USA. Y1 - 2005/05/26/ PY - 2005 DA - 2005 May 26 SP - 423 EP - 424 VL - 435 IS - 7041 KW - Antiviral Agents KW - 0 KW - Influenza Vaccines KW - Index Medicus KW - United States KW - Antiviral Agents -- therapeutic use KW - Animals KW - Biomedical Research -- trends KW - Humans KW - National Institutes of Health (U.S.) -- economics KW - Clinical Trials as Topic KW - Influenza Vaccines -- immunology KW - Influenza Vaccines -- economics KW - Population Surveillance KW - Genotype KW - Antiviral Agents -- supply & distribution KW - International Cooperation KW - Adult KW - Birds -- virology KW - Influenza Vaccines -- supply & distribution KW - Middle Aged KW - Antiviral Agents -- adverse effects KW - Adolescent KW - Time Factors KW - Orthomyxoviridae -- pathogenicity KW - Orthomyxoviridae -- genetics KW - Influenza, Human -- prevention & control KW - Influenza, Human -- epidemiology KW - Disaster Planning -- trends KW - Influenza, Human -- virology KW - Influenza, Human -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67869088?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Race+against+time.&rft.au=Fauci%2C+Anthony+S&rft.aulast=Fauci&rft.aufirst=Anthony&rft.date=2005-05-26&rft.volume=435&rft.issue=7041&rft.spage=423&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Uptake and neuritic transport of scrapie prion protein coincident with infection of neuronal cells. AN - 67874581; 15917460 AB - Invasion of the nervous system and neuronal spread of infection are critical, but poorly understood, steps in the pathogenesis of transmissible spongiform encephalopathies or prion diseases. To characterize pathways for the uptake and intraneuronal trafficking of infectious, protease-resistant prion protein (PrP-res), fluorescent-labeled PrP-res was used to infect a neuronally derived murine cell line (SN56) and adult hamster cortical neurons in primary culture. Concurrent with the establishment of persistent scrapie infection, SN56 cells internalized PrP-res aggregates into vesicles positive for markers for late endosomes and/or lysosomes but not synaptic, early endocytic, or raft-derived vesicles. Internalized PrP-res was then transported along neurites to points of contact with other cells. Similar trafficking was observed with dextran, Alzheimer's Abeta1-42 fibrils and noninfectious recombinant PrP fibrils, suggesting that PrP-res is internalized by a relatively nonspecific pinocytosis or transcytosis mechanism. Hamster cortical neurons were also capable of internalizing and disseminating exogenous PrP-res. Similar trafficking of exogenous PrP-res by cortical neurons cultured from the brains of PrP knock-out mice showed that uptake and neuritic transport did not require the presence of endogenous cellular PrP. These experiments visualize and characterize the initial steps associated with prion infection and transport within neuronal cells. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Magalhães, Ana Cristina AU - Baron, Gerald S AU - Lee, Kil Sun AU - Steele-Mortimer, Olivia AU - Dorward, David AU - Prado, Marco A M AU - Caughey, Byron AD - Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 SP - 5207 EP - 5216 VL - 25 IS - 21 KW - PrPSc Proteins KW - 0 KW - Green Fluorescent Proteins KW - 147336-22-9 KW - rab7 protein KW - 152989-05-4 KW - Cholera Toxin KW - 9012-63-9 KW - Endopeptidase K KW - EC 3.4.21.64 KW - rab GTP-Binding Proteins KW - EC 3.6.5.2 KW - Index Medicus KW - Animals KW - Cerebral Cortex -- cytology KW - Endopeptidase K -- pharmacology KW - Fluorescent Antibody Technique -- methods KW - Biological Transport KW - Mutagenesis -- physiology KW - Blotting, Western -- methods KW - Mice KW - rab GTP-Binding Proteins -- genetics KW - Diagnostic Imaging -- methods KW - Transfection -- methods KW - Cells, Cultured KW - rab GTP-Binding Proteins -- metabolism KW - Time Factors KW - Green Fluorescent Proteins -- metabolism KW - Protein Transport -- physiology KW - Cholera Toxin -- metabolism KW - Cricetinae KW - Neurons -- metabolism KW - Neurites -- virology KW - Nervous System Diseases -- metabolism KW - PrPSc Proteins -- metabolism KW - Neurons -- virology KW - Neurites -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67874581?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Uptake+and+neuritic+transport+of+scrapie+prion+protein+coincident+with+infection+of+neuronal+cells.&rft.au=Magalh%C3%A3es%2C+Ana+Cristina%3BBaron%2C+Gerald+S%3BLee%2C+Kil+Sun%3BSteele-Mortimer%2C+Olivia%3BDorward%2C+David%3BPrado%2C+Marco+A+M%3BCaughey%2C+Byron&rft.aulast=Magalh%C3%A3es&rft.aufirst=Ana&rft.date=2005-05-25&rft.volume=25&rft.issue=21&rft.spage=5207&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-21 N1 - Date created - 2005-05-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - Human genetic disorders of lymphocyte homeostasis AN - 40068262; 3938287 AU - Lenardo, MJ Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40068262?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Human+genetic+disorders+of+lymphocyte+homeostasis&rft.au=Lenardo%2C+MJ&rft.aulast=Lenardo&rft.aufirst=MJ&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: Keystone Symposia, 221 Summit Place #272, Drawer 1630, Silverthorne, CO 80498; phone: 970-262-1230; fax: 970-262-1525; email: info@keystonesymposia.org; URL: www.keystonesymposia.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Pulmonary function abnormalities in children with osteogenesis imperfecta correlate with OI type and location of collagen mutation AN - 40052544; 3939927 AU - Flor-Cisneros, A AU - Chaney, H AU - Vojtova, J AU - Marini, J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40052544?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Pulmonary+function+abnormalities+in+children+with+osteogenesis+imperfecta+correlate+with+OI+type+and+location+of+collagen+mutation&rft.au=Flor-Cisneros%2C+A%3BChaney%2C+H%3BVojtova%2C+J%3BMarini%2C+J&rft.aulast=Flor-Cisneros&rft.aufirst=A&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: American College of Medical Genetics, 9650 Rockville Pike, Bethesda, MD 20814, USA; phone: 301-634-7127; fax: 301-634-7275; email: acmg@acmg.net; URL: http://www.acmg.net/resources/ACGM/2005/2005-program-00.asp N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Validation status of the isolated chicken eye (ICE) test method AN - 40047087; 3931645 AU - Allen, D AU - Choksi, N AU - Inhof, C AU - Truax, J AU - Tice, R AU - Stokes, W Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40047087?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Validation+status+of+the+isolated+chicken+eye+%28ICE%29+test+method&rft.au=Allen%2C+D%3BChoksi%2C+N%3BInhof%2C+C%3BTruax%2C+J%3BTice%2C+R%3BStokes%2C+W&rft.aulast=Allen&rft.aufirst=D&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - 14-week comparative study of 1, 2, 3, 4, 6, 7-hexachloronaphthalene (PCN 66) and 1, 2, 3, 5, 6, 7-hexachloronaphthalene (PCN 67) in female harlan Sprague-Dawley (HSD) and Fischer 344 (F344) rats AN - 39994287; 3931708 AU - Hooth, M AU - Vallant, M AU - Walker, N AU - Nyska, A AU - Toyoshiba, H AU - Chhabra, R AU - Ryan, M AU - Vasconcelos, D AU - Hejtmancik, M AU - Fomby, L Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39994287?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C-dependent+regulation+of+NAG-1%2Fplacental+bone+morphogenic+protein%2FMIC-1+expression+in+LNCaP+prostate+carcinoma+cells.&rft.au=Shim%2C+Minsub%3BEling%2C+Thomas+E&rft.aulast=Shim&rft.aufirst=Minsub&rft.date=2005-05-13&rft.volume=280&rft.issue=19&rft.spage=18636&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Comparative analysis of gene networks at multiple doses and time points in livers of rats exposed to acetaminophen AN - 39957691; 3931014 AU - Sone, H AU - Toyoshiba, H AU - Yamanaka, T AU - Parham, F AU - Irwin, R AU - Boorman, G AU - Portier, C J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39957691?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Comparative+analysis+of+gene+networks+at+multiple+doses+and+time+points+in+livers+of+rats+exposed+to+acetaminophen&rft.au=Sone%2C+H%3BToyoshiba%2C+H%3BYamanaka%2C+T%3BParham%2C+F%3BIrwin%2C+R%3BBoorman%2C+G%3BPortier%2C+C+J&rft.aulast=Sone&rft.aufirst=H&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; 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phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Proteomic analysis of whole liver and subcellular fractions following acetaminophen-induced hepatotoxicity in male rats AN - 39895127; 3929702 AU - Bruno, ME AU - Wetmore, BA AU - Madenspacher, J H AU - Pieper, R AU - McGrath, A M AU - Gatlin, CL AU - Makusky, A J AU - Zhao, M AU - Zhou, J AU - Taylor, J Y1 - 2005/05/25/ PY - 2005 DA - 2005 May 25 KW - CPI, Conference Papers Index KW - U 2000:Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39895127?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Proteomic+analysis+of+whole+liver+and+subcellular+fractions+following+acetaminophen-induced+hepatotoxicity+in+male+rats&rft.au=Bruno%2C+ME%3BWetmore%2C+BA%3BMadenspacher%2C+J+H%3BPieper%2C+R%3BMcGrath%2C+A+M%3BGatlin%2C+CL%3BMakusky%2C+A+J%3BZhao%2C+M%3BZhou%2C+J%3BTaylor%2C+J&rft.aulast=Bruno&rft.aufirst=ME&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - SuppNotes - Availability: The Society of Toxicology, 1767 Business Center Drive, Suite 302, Resont, VA 20190-5332, USA; phone: 703-438-3115; fax: 703-438-3113; URL: http://www.toxicology.org N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - A Novel Algorithm for Microarray Time Series Data Analysis T2 - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AN - 39652265; 3949328 JF - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AU - McCall, Matthew N AU - Chen, Yidong AU - Davis, Sean AU - Walker, Robert L AU - Meltzer, Paul S Y1 - 2005/05/22/ PY - 2005 DA - 2005 May 22 KW - Algorithms KW - Data processing KW - U 5500:Geoscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39652265?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.atitle=A+Novel+Algorithm+for+Microarray+Time+Series+Data+Analysis&rft.au=McCall%2C+Matthew+N%3BChen%2C+Yidong%3BDavis%2C+Sean%3BWalker%2C+Robert+L%3BMeltzer%2C+Paul+S&rft.aulast=McCall&rft.aufirst=Matthew&rft.date=2005-05-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://dsplab.eng.umd.edu/gensips/program.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Using Genomic Data to Find Biological Mechanisms in Cancer: Beyond Expression Profiling T2 - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AN - 39647453; 3949281 JF - Third IEEE/EURASIP International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2005) AU - Meltzer, Paul Y1 - 2005/05/22/ PY - 2005 DA - 2005 May 22 KW - Profiling KW - Cancer KW - Genomics KW - U 5500:Geoscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39647453?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.atitle=Using+Genomic+Data+to+Find+Biological+Mechanisms+in+Cancer%3A+Beyond+Expression+Profiling&rft.au=Meltzer%2C+Paul&rft.aulast=Meltzer&rft.aufirst=Paul&rft.date=2005-05-22&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Third+IEEE%2FEURASIP+International+Workshop+on+Genomic+Signal+Processing+and+Statistics+%28GENSIPS+2005%29&rft.issn=&rft_id=info:doi/ L2 - http://dsplab.eng.umd.edu/gensips/program.html LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-21 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability. AN - 67949165; 15907206 AB - Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The effective correlation of observed changes in gene expression with shared transcription regulatory elements remains difficult to demonstrate convincingly. One reason for this difficulty may result from the intricate convergence of both transcriptional and mRNA turnover events which, together, directly influence steady-state mRNA levels. In order to investigate the relative contribution of gene transcription and changes in mRNA stability regulation to standard analyses of gene expression, we used two distinct microarray methods which individually measure nuclear gene transcription and changes in polyA mRNA gene expression. Gene expression profiles were obtained from both polyA mRNA (whole-cell) and nuclear run-on (newly transcribed) RNA across a time course of one hour following the activation of human Jurkat T cells with PMA plus ionomycin. Comparative analysis revealed that regulation of mRNA stability may account for as much as 50% of all measurements of changes in polyA mRNA in this system, as inferred by the absence of any corresponding regulation of nuclear gene transcription activity for these groups of genes. Genes which displayed dramatic elevations in both mRNA and nuclear run-on RNA were shown to be inhibited by Actinomycin D (ActD) pre-treatment of cells while large numbers of genes regulated only through altered mRNA turnover (both up and down) were ActD-resistant. Consistent patterns across the time course were observed for both transcribed and stability-regulated genes. We propose that regulation of mRNA stability contributes significantly to the observed changes in gene expression in response to external stimuli, as measured by high throughput systems. JF - BMC genomics AU - Cheadle, Chris AU - Fan, Jinshui AU - Cho-Chung, Yoon S AU - Werner, Thomas AU - Ray, Jill AU - Do, Lana AU - Gorospe, Myriam AU - Becker, Kevin G AD - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA. cheadlec@mail.nih.gov Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 75 VL - 6 KW - NF-kappa B KW - 0 KW - RNA, Messenger KW - Dactinomycin KW - 1CC1JFE158 KW - Poly A KW - 24937-83-5 KW - Ionomycin KW - 56092-81-0 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Oligonucleotide Array Sequence Analysis KW - Cell Nucleus -- metabolism KW - Humans KW - Computational Biology -- methods KW - Jurkat Cells KW - Ionomycin -- pharmacology KW - Poly A -- metabolism KW - Lymphocyte Activation KW - Dactinomycin -- pharmacology KW - Polymerase Chain Reaction KW - Tetradecanoylphorbol Acetate -- pharmacology KW - Time Factors KW - Cluster Analysis KW - NF-kappa B -- metabolism KW - T-Lymphocytes -- metabolism KW - RNA, Messenger -- metabolism KW - Genomics -- methods KW - Transcription, Genetic KW - Gene Expression Regulation KW - Gene Expression Profiling -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67949165?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+genomics&rft.atitle=Control+of+gene+expression+during+T+cell+activation%3A+alternate+regulation+of+mRNA+transcription+and+mRNA+stability.&rft.au=Cheadle%2C+Chris%3BFan%2C+Jinshui%3BCho-Chung%2C+Yoon+S%3BWerner%2C+Thomas%3BRay%2C+Jill%3BDo%2C+Lana%3BGorospe%2C+Myriam%3BBecker%2C+Kevin+G&rft.aulast=Cheadle&rft.aufirst=Chris&rft.date=2005-05-20&rft.volume=6&rft.issue=&rft.spage=75&rft.isbn=&rft.btitle=&rft.title=BMC+genomics&rft.issn=1471-2164&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-05-01 N1 - Date created - 2005-06-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nucleic Acids Res. 1999 Dec 1;27(23):4609-18 [10556317] Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 [9843981] Nat Genet. 2000 May;25(1):96-101 [10802665] J Biol Chem. 2000 Aug 11;275(32):24375-82 [10825165] Nucleic Acids Res. 2001 Jan 15;29(2):397-406 [11139609] Nat Immunol. 2001 Apr;2(4):316-24 [11276202] Eur J Cell Biol. 2001 May;80(5):321-8 [11432721] J Biol Chem. 2002 Feb 8;277(6):4465-76 [11694517] Restor Neurol Neurosci. 2001;18(2-3):127-35 [11847435] Nat Genet. 2002 May;31(1):19-20 [11984561] J Biol Chem. 2002 Jun 21;277(25):22175-84 [11943782] Plant J. 2002 Jul;31(2):171-88 [12121447] Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10611-6 [12149460] Nucleic Acids Res. 2002 Dec 15;30(24):5529-38 [12490721] J Mol Diagn. 2003 May;5(2):73-81 [12707371] Mol Cell. 2004 Jul 23;15(2):303-13 [15260981] Brief Funct Genomic Proteomic. 2004 Aug;3(2):112-24 [15355594] Science. 1989 Jan 20;243(4889):355-61 [2783497] J Immunol. 1989 Sep 15;143(6):1790-4 [2506269] J Biol Chem. 1991 Jul 5;266(19):12157-61 [2061303] Science. 1995 Oct 20;270(5235):467-70 [7569999] Mol Immunol. 1995 Sep;32(13):947-55 [7477000] Biotechniques. 1996 Apr;20(4):584-91 [8800675] Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10614-9 [8855227] Mol Cell Biol. 1998 Mar;18(3):1400-7 [9488455] Curr Opin Immunol. 2000 Apr;12(2):206-9 [10712937] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase I study of hepatic arterial melphalan infusion and hepatic venous hemofiltration using percutaneously placed catheters in patients with unresectable hepatic malignancies. AN - 67854233; 15908655 AB - We conducted a phase I study of a 30-minute hepatic artery infusion of melphalan via a percutaneously placed catheter and hepatic venous hemofiltration using a double balloon catheter positioned in the retrohepatic inferior vena cava to shunt hepatic venous effluent through an activated charcoal filter and then to the systemic circulation. The purpose of the study was to demonstrate feasibility in an initial cohort and subsequently determine the maximum tolerated dose and dose-limiting toxicity of melphalan. The initial cohort (n = 12) was treated with 2.0 mg/kg of melphalan before dose escalation to 3.5 mg/kg (n = 16). Total hepatic drug delivery, systemic levels, and percent filter efficiency were determined. Patients were assessed for hepatic and systemic toxicity and response. A total of 74 treatments were administered to 28 patients. Twelve patients with primary and metastatic hepatic tumors received 30 treatments (mean, 2.5 per patient) at an initial melphalan dose of 2.0 mg/kg. At 3.5 mg/kg, a dose-limiting toxicity (neutropenia and/or thrombocytopenia) was observed in two of six patients. Transient grade 3/4 hepatic and systemic toxicity was seen after 19% and 66% of treatments, respectively. An overall radiographic response rate of 30% was observed in treated patients. In the 10 patients with ocular melanoma, a 50% overall response rate was observed, including two complete responses. Delivery of melphalan via this system is feasible, with limited, manageable toxicity and evidence of substantial antitumor activity; 3 mg/kg is the maximum safe tolerated dose of melphalan administered via this technique. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Pingpank, James F AU - Libutti, Steven K AU - Chang, Richard AU - Wood, Bradford J AU - Neeman, Ziv AU - Kam, Anthony W AU - Figg, William D AU - Zhai, Souping AU - Beresneva, Tatiana AU - Seidel, Geoffrey D AU - Alexander, H Richard AD - Surgical Metabolism Section, Surgery Branch, National Cancer Institute/National Institutes of Health, Bethesda, MD 20892-1502, USA. Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 3465 EP - 3474 VL - 23 IS - 15 SN - 0732-183X, 0732-183X KW - Melphalan KW - Q41OR9510P KW - Index Medicus KW - Probability KW - Drug Administration Schedule KW - Infusions, Intra-Arterial KW - Neoplasm Staging KW - Combined Modality Therapy KW - Dose-Response Relationship, Drug KW - Humans KW - Terminally Ill KW - Aged KW - Palliative Care -- methods KW - Risk Assessment KW - Catheterization KW - Adult KW - Treatment Outcome KW - Middle Aged KW - Follow-Up Studies KW - Maximum Tolerated Dose KW - Female KW - Male KW - Survival Analysis KW - Liver Neoplasms -- pathology KW - Liver Neoplasms -- therapy KW - Melphalan -- administration & dosage KW - Liver Neoplasms -- mortality KW - Hemofiltration -- methods KW - Chemotherapy, Cancer, Regional Perfusion -- methods KW - Liver Neoplasms -- secondary UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67854233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Phase+I+study+of+hepatic+arterial+melphalan+infusion+and+hepatic+venous+hemofiltration+using+percutaneously+placed+catheters+in+patients+with+unresectable+hepatic+malignancies.&rft.au=Pingpank%2C+James+F%3BLibutti%2C+Steven+K%3BChang%2C+Richard%3BWood%2C+Bradford+J%3BNeeman%2C+Ziv%3BKam%2C+Anthony+W%3BFigg%2C+William+D%3BZhai%2C+Souping%3BBeresneva%2C+Tatiana%3BSeidel%2C+Geoffrey+D%3BAlexander%2C+H+Richard&rft.aulast=Pingpank&rft.aufirst=James&rft.date=2005-05-20&rft.volume=23&rft.issue=15&rft.spage=3465&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-24 N1 - Date created - 2005-05-23 N1 - Date revised - 2017-01-14 N1 - SuppNotes - Cited By: J Clin Oncol. 2003 Jun 1;21(11):2059-69 [12775730] CA Cancer J Clin. 2003 Jan-Feb;53(1):5-26 [12568441] Surgery. 2001 Oct;130(4):677-82; discussion 682-5 [11602899] J Clin Oncol. 2001 May 15;19(10):2687-95 [11352961] J Am Coll Surg. 2000 Nov;191(5):519-30 [11085732] Oncologist. 2000;5(5):416-24 [11040278] Clin Cancer Res. 2000 Aug;6(8):3062-70 [10955785] Br J Cancer. 1982 Jan;45(1):17-26 [7059461] Cancer. 1983 Jul 15;52(2):334-6 [6190546] Surgery. 1986 Aug;100(2):285-91 [2943036] Surv Ophthalmol. 1988 Jan-Feb;32(4):239-51 [3279559] Ophthalmology. 1991 Mar;98(3):383-9; discussion 390 [2023760] Surgery. 1993 Sep;114(3):579-85 [8367814] Cancer. 1993 Oct 1;72(7):2219-23 [7848381] Cancer Chemother Pharmacol. 1993;33(3):251-7 [8269607] Int J Hyperthermia. 1994 Jan-Feb;10(1):89-99 [7511674] J Nucl Med. 1994 Oct;35(10):1637-44 [7931662] J Clin Oncol. 1994 Dec;12(12):2723-36 [7989950] Bone Marrow Transplant. 1994 Sep;14(3):443-8 [7994270] N Engl J Med. 1995 May 11;332(19):1256-61 [7708069] J Clin Oncol. 1995 Jul;13(7):1786-99 [7602368] J Clin Oncol. 1997 Jun;15(6):2420-31 [9196158] J Clin Oncol. 1998 Apr;16(4):1479-89 [9552055] Ann Surg. 1999 Jul;230(1):1-8 [10400029] Ann Surg. 1999 Sep;230(3):309-18; discussion 318-21 [10493478] N1 - Last updated - 2017-01-19 ER - TY - JOUR T1 - Investigating HIV-1 polypurine tract geometry via targeted insertion of abasic lesions in the (-)-DNA template and (+)-RNA primer. AN - 67829396; 15778225 AB - A variety of biochemical and structural studies indicate that two regions of the human immunodeficiency virus type 1 (HIV-1) polypurine tract (PPT)-containing RNA/DNA hybrid deviate from standard Watson-Crick geometry. However, it is unclear whether and how these regions cooperate to ensure PPT primer selection by reverse transcriptase-associated ribonuclease H and subsequent removal from nascent (+)-DNA. To address these issues, we synthesized oligonucleotides containing abasic lesions in either the PPT (+)-RNA primer or (-)-DNA template to locally remove nucleobases, although retaining the sugar-phosphate backbone. KMnO(4) footprinting indicates such lesions locally alter duplex structure, whereas thermal melting studies show significantly reduced stability when lesions are positioned around the scissile bond. Substituting the (-)-DNA template between positions -15 and -13 altered cleavage specificity, whereas equivalent substitutions of the (+)-RNA had almost no effect. The unpaired base of the DNA template observed crystallographically (-11C) could also be removed without significant loss of cleavage specificity. With respect to the scissile -1/+1 phosphodiester bond, template nucleobases could be removed without loss of cleavage specificity, whereas equivalent lesions in the RNA primer were inhibitory. Our data suggest an interaction between the p66 thumb subdomain of HIV-1 reverse transcriptase, and the DNA template in the "unzipped" portion of the RNA/DNA hybrid could aid in positioning the ribonuclease H catalytic center at the PPT/U3 junction and also provides insights into nucleic acid geometry around the scissile bond required for hydrolysis. JF - The Journal of biological chemistry AU - Yi-Brunozzi, Hye Young AU - Le Grice, Stuart F J AD - Reverse Transcriptase Biochemistry Section, Resistance Mechanisms Laboratory, HIV Drug Resistance Program, NCI, National Institutes of Health, Frederick, Maryland 21702, USA. Y1 - 2005/05/20/ PY - 2005 DA - 2005 May 20 SP - 20154 EP - 20162 VL - 280 IS - 20 SN - 0021-9258, 0021-9258 KW - DNA, Viral KW - 0 KW - Purines KW - RNA primers KW - RNA, Viral KW - RNA KW - 63231-63-0 KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - Index Medicus KW - Base Sequence KW - In Vitro Techniques KW - HIV Reverse Transcriptase -- metabolism KW - Reverse Transcription KW - Binding Sites -- genetics KW - Purines -- chemistry KW - Nucleic Acid Conformation KW - Models, Biological KW - Mutagenesis, Insertional KW - HIV-1 -- metabolism KW - HIV-1 -- genetics KW - HIV-1 -- chemistry KW - DNA, Viral -- chemistry KW - RNA, Viral -- chemistry KW - RNA, Viral -- genetics KW - RNA, Viral -- metabolism KW - DNA, Viral -- genetics KW - DNA, Viral -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829396?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Investigating+HIV-1+polypurine+tract+geometry+via+targeted+insertion+of+abasic+lesions+in+the+%28-%29-DNA+template+and+%28%2B%29-RNA+primer.&rft.au=Yi-Brunozzi%2C+Hye+Young%3BLe+Grice%2C+Stuart+F+J&rft.aulast=Yi-Brunozzi&rft.aufirst=Hye&rft.date=2005-05-20&rft.volume=280&rft.issue=20&rft.spage=20154&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The decrease in the presynaptic calcium current is a major cause of short-term depression at a calyx-type synapse. AN - 67915862; 15944131 AB - Repetitive nerve firings cause short-term depression (STD) of release at many synapses. Its underlying mechanism is largely attributed to depletion of a readily releasable vesicle pool (RRP) and a decreased probability of releasing a readily releasable vesicle during an action potential. Which of these two mechanisms is dominant and the mechanism that decreases the release probability remain debated. Here, we report that a decreased release probability is caused by a calcium-induced inhibition of presynaptic calcium channels, particularly P/Q-type channels at the calyx of Held in rat brainstem. This mechanism was the dominant cause of STD in a wide range of stimulation conditions, such as during 2 to 20 action potential-equivalent stimuli (AP-e) at 0.2-30 Hz and after 2 to 20 AP-e at 0.2-100 Hz. Only during > or = 100 Hz AP-e was depletion the dominant mechanism. JF - Neuron AU - Xu, Jianhua AU - Wu, Ling-Gang AD - National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA. Y1 - 2005/05/19/ PY - 2005 DA - 2005 May 19 SP - 633 EP - 645 VL - 46 IS - 4 SN - 0896-6273, 0896-6273 KW - Calcium Channels KW - 0 KW - Chelating Agents KW - Enzyme Inhibitors KW - Imidazoles KW - Nuclear Proteins KW - Peptides KW - Trans-Activators KW - MLCK peptide KW - 135467-90-2 KW - Barium KW - 24GP945V5T KW - calmidazolium KW - 4R9H38JAWL KW - Egtazic Acid KW - 526U7A2651 KW - CREB-Binding Protein KW - EC 2.3.1.48 KW - Crebbp protein, rat KW - 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid KW - K22DDW77C0 KW - Calcium KW - SY7Q814VUP KW - Index Medicus KW - Patch-Clamp Techniques -- methods KW - Electric Capacitance KW - Nuclear Proteins -- pharmacology KW - Action Potentials -- physiology KW - Animals KW - Electric Stimulation -- methods KW - Trans-Activators -- pharmacology KW - Imidazoles -- pharmacology KW - Action Potentials -- radiation effects KW - Action Potentials -- drug effects KW - Dose-Response Relationship, Radiation KW - Peptides -- pharmacology KW - Models, Biological KW - Rats KW - Animals, Newborn KW - Chelating Agents -- pharmacology KW - In Vitro Techniques KW - Rats, Wistar KW - Enzyme Inhibitors -- pharmacology KW - Brain Stem -- cytology KW - Time Factors KW - Barium -- pharmacology KW - Calcium -- metabolism KW - Presynaptic Terminals -- drug effects KW - Egtazic Acid -- analogs & derivatives KW - Calcium Channels -- radiation effects KW - Calcium Channels -- metabolism KW - Neural Inhibition -- radiation effects KW - Calcium Channels -- drug effects KW - Neural Inhibition -- drug effects KW - Synapses -- metabolism KW - Neural Inhibition -- physiology KW - Egtazic Acid -- pharmacology KW - Presynaptic Terminals -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67915862?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuron&rft.atitle=The+decrease+in+the+presynaptic+calcium+current+is+a+major+cause+of+short-term+depression+at+a+calyx-type+synapse.&rft.au=Xu%2C+Jianhua%3BWu%2C+Ling-Gang&rft.aulast=Xu&rft.aufirst=Jianhua&rft.date=2005-05-19&rft.volume=46&rft.issue=4&rft.spage=633&rft.isbn=&rft.btitle=&rft.title=Neuron&rft.issn=08966273&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-29 N1 - Date created - 2005-06-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Neuron. 2005 May 19;46(4):523-5 [15944119] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Coronary heart disease in menopausal women: implications of primary and secondary prevention trials of hormones. AN - 67809984; 15883110 AB - In direct contrast to the observational studies, both primary and secondary prevention trials of female reproductive hormones have found no benefit for coronary heart disease (CHD). Basic science studies have elucidated several mechanisms by which estrogen may improve coronary arterial physiology and prevent pathology, but have also found mechanisms by which estrogen might increase coagulation or inflammation, or might trigger coronary events in advanced lesions. Animal studies suggest that hormones may retard early atherosclerosis, while both animal studies and human angiographic trials are conclusive that hormones do not retard progression of raised lesions. Hormone use in the primary prevention observational studies would mostly have started at the age of menopause, in women whose arteries on average would be closer to normal than those of women in the clinical trials. One hypothesis worthy of further study is that estrogen may have a beneficial effect in normal or near-normal arteries, but the opposite effect in the presence of established atherosclerosis. However, at the average age of menopause, a substantial proportion of women has raised lesions, and a smaller proportion already has advanced lesions. Also, the apparent benefit of hormone use was found in secondary prevention observational studies, i.e., in women with compromised arteries. It is likely that uncorrected biases in the observational studies lead to an overestimation of any benefit of hormone use. On the other hand, endogenous estradiol may be responsible for the later onset of coronary disease in women compared to men; if so, then the appropriate test of the estrogen hypothesis would employ transdermal estradiol in a young population of menopausal women. Hormones are not indicated for the prevention of CHD, particularly in the light of the increased risk for stroke and venous thrombosis. Their use for other indications (menopausal symptoms, osteoporosis) needs to be tempered by the risk for cardiovascular disease (CVD). JF - Maturitas AU - Rossouw, Jacques E AD - National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-7966, USA. rossouwj@nih.gov Y1 - 2005/05/16/ PY - 2005 DA - 2005 May 16 SP - 51 EP - 63 VL - 51 IS - 1 SN - 0378-5122, 0378-5122 KW - Index Medicus KW - Blood Coagulation Disorders -- chemically induced KW - Animals KW - Randomized Controlled Trials as Topic KW - Angiography KW - Humans KW - Inflammation -- chemically induced KW - Aged KW - Middle Aged KW - Female KW - Atherosclerosis -- chemically induced KW - Primary Prevention KW - Hormone Replacement Therapy -- adverse effects KW - Coronary Disease -- prevention & control KW - Menopause -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67809984?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Maturitas&rft.atitle=Coronary+heart+disease+in+menopausal+women%3A+implications+of+primary+and+secondary+prevention+trials+of+hormones.&rft.au=Rossouw%2C+Jacques+E&rft.aulast=Rossouw&rft.aufirst=Jacques&rft.date=2005-05-16&rft.volume=51&rft.issue=1&rft.spage=51&rft.isbn=&rft.btitle=&rft.title=Maturitas&rft.issn=03785122&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-22 N1 - Date created - 2005-05-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibitors of the arachidonic acid pathway and peroxisome proliferator-activated receptor ligands have superadditive effects on lung cancer growth inhibition. AN - 67837647; 15899809 AB - Arachidonic acid (AA) metabolizing enzymes and peroxisome proliferator-activated receptors (PPARs) have been shown to regulate the growth of epithelial cells. We have previously reported that exposure to the 5-lipoxygenase activating protein-directed inhibitor MK886 but not the cyclooxygenase inhibitor, indomethacin, reduced growth, increased apoptosis, and up-regulated PPARalpha and gamma expression in breast cancer cell lines. In the present study, we explore approaches to maximizing the proapoptotic effects of PPARgamma on lung cancer cell lines. Non-small-cell cancer cell line A549 revealed dose-dependent PPARgamma reporter activity after treatment with MK886. The addition of indomethacin in combination with MK886 further increases reporter activity. We also show increased growth inhibition and up-regulation of apoptosis after exposure to MK886 alone, or in combination with indomethacin and the PPAR ligand, 15-deoxy-Delta12,14-prostaglandin J2 compared with single drug exposures on the adenocarcinoma cell line A549 and small-cell cancer cell lines H345, N417, and H510. Real-time PCR analyses showed increased PPAR mRNA and retinoid X receptor (RXR)alpha mRNA expression after exposure to MK886 and indomethacin in a time-dependent fashion. The results suggest that the principal proapoptotic effect of these drugs may be mediated through the known antiproliferative effects of the PPARgamma-RXR interaction. We therefore explored a three-drug approach to attempt to maximize this effect. The combination of low-dose MK886, ciglitazone, and 13-cis-retinoic acid interacted at least in a superadditive fashion to inhibit the growth of lung cancer cell lines A549 and H1299, suggesting that targeting PPARgamma and AA action is a promising approach to lung cancer growth with a favorable therapeutic index. JF - Cancer research AU - Avis, Ingalill AU - Martínez, Alfredo AU - Tauler, Jordi AU - Zudaire, Enrique AU - Mayburd, Anatoly AU - Abu-Ghazaleh, Raed AU - Ondrey, Frank AU - Mulshine, James L AD - Intervention Section, Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 4181 EP - 4190 VL - 65 IS - 10 SN - 0008-5472, 0008-5472 KW - Acetophenones KW - 0 KW - Indoles KW - Ligands KW - Peroxisome Proliferator-Activated Receptors KW - Pyrimidines KW - RNA, Messenger KW - Retinoid X Receptor alpha KW - Tetrazoles KW - Thiazolidinediones KW - MK-886 KW - 080626SQ8C KW - Arachidonic Acid KW - 27YG812J1I KW - 9-deoxy-delta-9-prostaglandin D2 KW - 60203-57-8 KW - pirinixic acid KW - 86C4MRT55A KW - LY 171883 KW - 88107-10-2 KW - Caspases KW - EC 3.4.22.- KW - Isotretinoin KW - EH28UP18IF KW - Prostaglandin D2 KW - RXY07S6CZ2 KW - ciglitazone KW - U8QXS1WU8G KW - Index Medicus KW - Retinoid X Receptor alpha -- biosynthesis KW - Cell Growth Processes -- physiology KW - Dose-Response Relationship, Drug KW - Retinoid X Receptor alpha -- genetics KW - Humans KW - Cell Line, Tumor KW - Tetrazoles -- administration & dosage KW - RNA, Messenger -- genetics KW - Pyrimidines -- administration & dosage KW - RNA, Messenger -- biosynthesis KW - Caspases -- metabolism KW - Isotretinoin -- administration & dosage KW - Acetophenones -- administration & dosage KW - Apoptosis -- drug effects KW - Enzyme Activation -- drug effects KW - Indoles -- administration & dosage KW - Thiazolidinediones -- administration & dosage KW - Arachidonic Acid -- antagonists & inhibitors KW - Peroxisome Proliferator-Activated Receptors -- biosynthesis KW - Prostaglandin D2 -- analogs & derivatives KW - Lung Neoplasms -- drug therapy KW - Peroxisome Proliferator-Activated Receptors -- metabolism KW - Antineoplastic Combined Chemotherapy Protocols -- pharmacology KW - Prostaglandin D2 -- administration & dosage KW - Peroxisome Proliferator-Activated Receptors -- genetics KW - Arachidonic Acid -- metabolism KW - Lung Neoplasms -- pathology KW - Lung Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67837647?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Inhibitors+of+the+arachidonic+acid+pathway+and+peroxisome+proliferator-activated+receptor+ligands+have+superadditive+effects+on+lung+cancer+growth+inhibition.&rft.au=Avis%2C+Ingalill%3BMart%C3%ADnez%2C+Alfredo%3BTauler%2C+Jordi%3BZudaire%2C+Enrique%3BMayburd%2C+Anatoly%3BAbu-Ghazaleh%2C+Raed%3BOndrey%2C+Frank%3BMulshine%2C+James+L&rft.aulast=Avis&rft.aufirst=Ingalill&rft.date=2005-05-15&rft.volume=65&rft.issue=10&rft.spage=4181&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-07 N1 - Date created - 2005-05-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Breast cancer cells induce stromal fibroblasts to express MMP-9 via secretion of TNF-alpha and TGF-beta. AN - 67830058; 15855236 AB - We used 2D-cocultures employing fibroblasts of different genetic backgrounds and MCF10A-derived human breast epithelial cells of increasingly malignant potential to investigate tumor-stroma interactions in breast cancer and to identify possible signaling pathways involved. Tumor cells induced expression of matrix-metalloproteinase 9 (MMP-9) in fibroblasts in a pattern dependent on the degree of their malignancy. In-situ zymography localized the main gelatinolytic activity around stromal cells in cocultures and xenografted tumors. Use of Smad3 knockout fibroblasts, small molecule inhibitors, and neutralizing antibodies showed that MMP-9 expression was induced by tumor cell-derived TNF-alpha and TGF-beta, dependent on Smad-, Ras-, and PI3-kinase-signaling, and likewise modulated by subsequent HGF- and EGF-signaling. Together, our results indicate that MMP-9 levels in tumor fibroblasts are regulated by a complex tumor-stroma cross-talk, involving multiple ligands and cellular signaling pathways. JF - Journal of cell science AU - Stuelten, Christina H AU - DaCosta Byfield, Stacey AU - Arany, Praveen R AU - Karpova, Tatiana S AU - Stetler-Stevenson, William G AU - Roberts, Anita B AD - Laboratory of Cell Regulation and Carcinogenesis, CCR, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 2143 EP - 2153 VL - 118 SN - 0021-9533, 0021-9533 KW - Smad3 Protein KW - 0 KW - Smad3 protein, mouse KW - Transforming Growth Factor beta KW - Tumor Necrosis Factor-alpha KW - Epidermal Growth Factor KW - 62229-50-9 KW - Protein Kinases KW - EC 2.7.- KW - Phosphatidylinositol 3-Kinases KW - EC 2.7.1.- KW - Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Index Medicus KW - Animals KW - Coculture Techniques KW - Humans KW - Phosphatidylinositol 3-Kinases -- physiology KW - Cell Line, Tumor KW - Mice KW - Mitogen-Activated Protein Kinases -- physiology KW - Smad3 Protein -- genetics KW - Mice, Knockout KW - Epithelial Cells -- metabolism KW - Neoplasm Transplantation KW - Signal Transduction -- physiology KW - Protein Kinases -- metabolism KW - Epithelial Cells -- secretion KW - Transplantation, Heterologous KW - Stromal Cells -- metabolism KW - Epidermal Growth Factor -- physiology KW - Matrix Metalloproteinase 9 -- metabolism KW - Breast Neoplasms -- pathology KW - Transforming Growth Factor beta -- secretion KW - Breast Neoplasms -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Transforming Growth Factor beta -- metabolism KW - Fibroblasts -- metabolism KW - Tumor Necrosis Factor-alpha -- secretion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67830058?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+cell+science&rft.atitle=Breast+cancer+cells+induce+stromal+fibroblasts+to+express+MMP-9+via+secretion+of+TNF-alpha+and+TGF-beta.&rft.au=Stuelten%2C+Christina+H%3BDaCosta+Byfield%2C+Stacey%3BArany%2C+Praveen+R%3BKarpova%2C+Tatiana+S%3BStetler-Stevenson%2C+William+G%3BRoberts%2C+Anita+B&rft.aulast=Stuelten&rft.aufirst=Christina&rft.date=2005-05-15&rft.volume=118&rft.issue=&rft.spage=2143&rft.isbn=&rft.btitle=&rft.title=Journal+of+cell+science&rft.issn=00219533&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-01-11 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - SLC18A2 promoter haplotypes and identification of a novel protective factor against alcoholism. AN - 67829328; 15829504 AB - The vesicular monoamine transporter 2 (VMAT2, SLC18A2) takes up cytosolic monoamines into intracellular secretory vesicles, preventing their neurotoxicity in the cytosol and discharging them into extracellular space by exocytosis. It has been shown that one-copy deletion of the VMAT2 gene increases locomotion activity significantly in response to drug treatments and dopamine neuron death rate in response to neurotoxin treatments in knockout mice. Little is known about promoter polymorphisms and their influence on SLC18A2 promoter activity. We have re-sequenced a 17.4 kb DNA in the SLC18A2 promoter region for Caucasians and revealed 47 polymorphisms that confer 13 haplotypes. One of the haplotypes reaches a frequency as high as 65%, likely due to positive selection. In vitro analysis showed a 20% difference in promoter activity between two frequent haplotypes and identified some of the polymorphisms that influence promoter activity. Four haplotype-defining single nucleotide polymorphisms (hdSNPs) can define the frequent haplotypes and by genotyping these hdSNPs, we find that haplotypes with -14234G and -2504C of SLC18A2 promoter region represent a protective factor against alcoholism (P = 0.0038 by Fisher's exact tests). Therefore, SLC18A2 promoter haplotypes defined here create a foundation for transcriptional characterization of individuality and for association study on monoamine-related human diseases. JF - Human molecular genetics AU - Lin, Zhicheng AU - Walther, Donna AU - Yu, Xiao-Ying AU - Li, Suxia AU - Drgon, Tomas AU - Uhl, George R AD - Molecular Neurobiology Branch, Baltimore, MD 21224, USA. zlin@intra.nida.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1393 EP - 1404 VL - 14 IS - 10 SN - 0964-6906, 0964-6906 KW - Membrane Glycoproteins KW - 0 KW - Membrane Transport Proteins KW - SLC18A2 protein, human KW - Slc18a2 protein, mouse KW - Vesicular Biogenic Amine Transport Proteins KW - Vesicular Monoamine Transport Proteins KW - Index Medicus KW - Animals KW - Haplotypes KW - Polymorphism, Genetic KW - Humans KW - Mice KW - Selection, Genetic KW - Promoter Regions, Genetic KW - Alcoholism -- genetics KW - Membrane Transport Proteins -- metabolism KW - Membrane Transport Proteins -- genetics KW - Membrane Glycoproteins -- metabolism KW - Membrane Glycoproteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829328?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+molecular+genetics&rft.atitle=SLC18A2+promoter+haplotypes+and+identification+of+a+novel+protective+factor+against+alcoholism.&rft.au=Lin%2C+Zhicheng%3BWalther%2C+Donna%3BYu%2C+Xiao-Ying%3BLi%2C+Suxia%3BDrgon%2C+Tomas%3BUhl%2C+George+R&rft.aulast=Lin&rft.aufirst=Zhicheng&rft.date=2005-05-15&rft.volume=14&rft.issue=10&rft.spage=1393&rft.isbn=&rft.btitle=&rft.title=Human+molecular+genetics&rft.issn=09646906&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-26 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Multiple costimulatory modalities enhance CTL avidity. AN - 67806642; 15879092 AB - Recent studies in both animal models and clinical trials have demonstrated that the avidity of T cells is a major determinant of antitumor and antiviral immunity. In this study, we evaluated several different vaccine strategies for their ability to enhance both the quantity and avidity of CTL responses. CD8(+) T cell quantity was measured by tetramer binding precursor frequency, and avidity was measured by both tetramer dissociation and quantitative cytolytic function. We have evaluated a peptide, a viral vector expressing the Ag transgene alone, with one costimulatory molecule (B7-1), and with three costimulatory molecules (B7-1, ICAM-1, and LFA-3), with anti-CTLA-4 mAb, with GM-CSF, and combinations of the above. We have evaluated these strategies in both a foreign Ag model using beta-galactosidase as immunogen, and in a "self" Ag model, using carcinoembryonic Ag as immunogen in carcinoembryonic Ag transgenic mice. The combined use of several of these strategies was shown to enhance not only the quantity, but, to a greater magnitude, the avidity of T cells generated; a combination strategy is also shown to enhance antitumor effects. The results reported in this study thus demonstrate multiple strategies that can be used in both antitumor and antiviral vaccine settings to generate higher avidity host T cell responses. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Hodge, James W AU - Chakraborty, Mala AU - Kudo-Saito, Chie AU - Garnett, Charlie T AU - Schlom, Jeffrey AD - Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 5994 EP - 6004 VL - 174 IS - 10 SN - 0022-1767, 0022-1767 KW - Adjuvants, Immunologic KW - 0 KW - Antibodies, Monoclonal KW - Antigens, CD KW - Antigens, Differentiation KW - CTLA-4 Antigen KW - Cancer Vaccines KW - Carcinoembryonic Antigen KW - Ctla4 protein, mouse KW - Peptide Fragments KW - Granulocyte-Macrophage Colony-Stimulating Factor KW - 83869-56-1 KW - beta-Galactosidase KW - EC 3.2.1.23 KW - Abridged Index Medicus KW - Index Medicus KW - beta-Galactosidase -- administration & dosage KW - Animals KW - Mice, Transgenic KW - Antibodies, Monoclonal -- immunology KW - Granulocyte-Macrophage Colony-Stimulating Factor -- administration & dosage KW - Genetic Vectors KW - Colonic Neoplasms -- pathology KW - Peptide Fragments -- metabolism KW - Carcinoembryonic Antigen -- administration & dosage KW - Carcinoembryonic Antigen -- immunology KW - Combined Modality Therapy KW - Antigens, Differentiation -- immunology KW - Cell Line, Tumor KW - Mice KW - beta-Galactosidase -- immunology KW - Colonic Neoplasms -- immunology KW - Peptide Fragments -- immunology KW - Protein Binding -- immunology KW - Antibodies, Monoclonal -- administration & dosage KW - Mice, Inbred C57BL KW - beta-Galactosidase -- genetics KW - Colonic Neoplasms -- prevention & control KW - Peptide Fragments -- administration & dosage KW - Carcinoembryonic Antigen -- genetics KW - Female KW - Cytotoxicity Tests, Immunologic -- methods KW - Cancer Vaccines -- administration & dosage KW - Lymphocyte Activation -- genetics KW - Cancer Vaccines -- immunology KW - Adjuvants, Immunologic -- administration & dosage KW - Lymphocyte Activation -- immunology KW - Cancer Vaccines -- genetics KW - T-Lymphocytes, Cytotoxic -- immunology KW - Adjuvants, Immunologic -- genetics KW - T-Lymphocytes, Cytotoxic -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67806642?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Multiple+costimulatory+modalities+enhance+CTL+avidity.&rft.au=Hodge%2C+James+W%3BChakraborty%2C+Mala%3BKudo-Saito%2C+Chie%3BGarnett%2C+Charlie+T%3BSchlom%2C+Jeffrey&rft.aulast=Hodge&rft.aufirst=James&rft.date=2005-05-15&rft.volume=174&rft.issue=10&rft.spage=5994&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cancer Res. 2001 Oct 15;61(20):7568-76 [11606396] J Immunol. 2001 Nov 15;167(10):5824-31 [11698456] J Immunol. 2002 Feb 1;168(3):1167-71 [11801651] Immunity. 2002 Jan;16(1):23-35 [11825563] Nat Rev Immunol. 2001 Dec;1(3):209-19 [11905830] J Immunol. 2002 Jul 1;169(1):531-9 [12077285] Nat Immunol. 2002 Jul;3(7):611-8 [12087419] Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9358-63 [12093915] Cancer Res. 2002 Oct 15;62(20):5770-7 [12384537] J Immunol. 2003 Mar 1;170(5):2523-30 [12594278] Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8372-7 [12826605] J Immunol. 2003 Sep 1;171(5):2427-34 [12928390] Nat Med. 2003 Nov;9(11):1377-82 [14528297] Clin Cancer Res. 2004 Feb 1;10(3):1090-9 [14871989] Curr HIV Res. 2003 Jul;1(3):287-94 [15046253] J Virol. 2004 Jul;78(13):7052-60 [15194781] Cancer Res. 1991 Jul 15;51(14):3657-62 [1712245] J Natl Cancer Inst. 1992 Jul 15;84(14):1084-91 [1619682] Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3539-43 [8097319] Cancer Res. 1994 Aug 1;54(15):4169-76 [8033149] J Exp Med. 1995 Aug 1;182(2):459-65 [7543139] Nature. 1995 Sep 28;377(6547):348-51 [7566090] Science. 1996 Mar 22;271(5256):1734-6 [8596936] Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4102-7 [8633023] Blood. 1996 Jul 1;88(1):202-10 [8704175] Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10972-7 [8855293] Methods. 1997 Jun;12(2):117-23 [9184376] Cancer Res. 1997 Sep 15;57(18):4036-41 [9307290] Ann Surg Oncol. 1997 Oct-Nov;4(7):579-85 [9367025] Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10067-71 [9707601] J Clin Invest. 1998 Sep 15;102(6):1239-48 [9739058] Novartis Found Symp. 1998;215:92-8; discussion 98-102, 186-90 [9760573] J Immunol. 1999 Jan 15;162(2):989-94 [9916724] Cancer Res. 1999 Feb 1;59(3):676-83 [9973217] J Immunol. 1999 Feb 15;162(4):2227-34 [9973498] Vaccine. 1999 Feb 26;17(7-8):893-903 [10067696] Cancer Gene Ther. 1999 Jan-Feb;6(1):89-95 [10078968] Cancer Immunol Immunother. 1999 May-Jun;48(2-3):123-31 [10414466] J Exp Med. 1999 Aug 2;190(3):355-66 [10430624] J Clin Oncol. 2005 Feb 1;23(4):720-31 [15613691] Immunol Rev. 1999 Aug;170:151-72 [10566149] Cancer Res. 1999 Nov 15;59(22):5800-7 [10582702] J Immunol. 2000 Jan 1;164(1):191-200 [10605011] Cancer Res. 2000 May 1;60(9):2444-8 [10811122] Cytokine. 2000 Jul;12(7):960-71 [10880241] J Exp Med. 2000 Jul 17;192(2):295-302 [10899916] J Natl Cancer Inst. 2000 Aug 2;92(15):1228-39 [10922408] Cancer Res. 2001 Jan 1;61(1):206-14 [11196163] Eur J Immunol. 2001 Feb;31(2):412-20 [11180105] J Immunol. 2001 Feb 1;166(3):1690-7 [11160212] J Immunol. 2001 Mar 15;166(6):3908-14 [11238635] Int Immunol. 2001 Jun;13(6):817-24 [11369710] Int Immunol. 2001 Jul;13(7):897-908 [11431420] Cancer Res. 2001 Aug 1;61(15):5850-6 [11479225] J Exp Med. 2001 Aug 20;194(4):481-9 [11514604] Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10302-7 [11517329] J Exp Med. 2001 Sep 17;194(6):823-32 [11560997] J Exp Med. 2001 Sep 17;194(6):833-46 [11560998] J Virol. 2001 Nov;75(21):10065-72 [11581375] Erratum In: J Immunol. 2005 Jun 15;174(12):8220 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling. AN - 67806210; 15879121 AB - Phospholipase Cgamma (PLCgamma) is a ubiquitous gatekeeper of calcium mobilization and diacylglycerol-mediated events induced by the activation of Ag and growth factor receptors. The activity of PLCgamma is regulated through its controlled membrane translocation and tyrosine (Y) phosphorylation. Four activation-induced tyrosine phosphorylation sites have been previously described (Y472, Y771, Y783, and Y1254), but their specific roles in Ag receptor-induced PLCgamma1 activation are not fully elucidated. Unexpectedly, we found that the phosphorylation of a PLCgamma1 construct with all four sites mutated to phenylalanine was comparable with that observed with wild-type PLCgamma1, suggesting the existence of an unidentified site(s). Sequence alignment with known phosphorylation sites in PLCgamma2 indicated homology of PLCgamma1 tyrosine residue 775 (Y775) with PLCgamma2 Y753, a characterized phosphorylation site. Tyrosine 775 was characterized as a phosphorylation site using phospho-specific anti-Y775 antiserum, and by mutational analysis. Phosphorylation of Y775 did not depend on the other tyrosines, and point mutation of PLCgamma1 Y775, or the previously described Y783, substantially reduced AgR-induced calcium, NF-AT, and AP-1 activation. Mutation of Y472, Y771, and Y1254 had no effect on overall PLCgamma1 phosphorylation or activation. Although the concomitant mutation of Y775 and Y783 abolished downstream PLCgamma1 signaling, these two tyrosines were sufficient to reconstitute the wild-type response in the absence of functional Y472, Y771, and Y1254. These data establish Y775 as a critical phosphorylation site for PLCgamma1 activation and confirm the functional importance of Y783. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Serrano, Carmen J AU - Graham, Laurie AU - DeBell, Karen AU - Rawat, Rashmi AU - Veri, Maria Concetta AU - Bonvini, Ezio AU - Rellahan, Barbara L AU - Reischl, Ilona G AD - Division of Monoclonal Antibodies, Office of Biotechnology Products, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, National Institutes of Health Campus, Bethesda, MD 20892, USA. Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 6233 EP - 6237 VL - 174 IS - 10 SN - 0022-1767, 0022-1767 KW - DNA-Binding Proteins KW - 0 KW - Diglycerides KW - Isoenzymes KW - NFATC Transcription Factors KW - Nuclear Proteins KW - Receptors, Antigen, B-Cell KW - Receptors, Antigen, T-Cell KW - Transcription Factor AP-1 KW - Transcription Factors KW - Tyrosine KW - 42HK56048U KW - Type C Phospholipases KW - EC 3.1.4.- KW - Phospholipase C gamma KW - EC 3.1.4.3 KW - Calcium KW - SY7Q814VUP KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Transcription Factors -- metabolism KW - Transcription Factor AP-1 -- metabolism KW - Humans KW - Jurkat Cells KW - Amino Acid Sequence KW - Isoenzymes -- genetics KW - Isoenzymes -- metabolism KW - Mutagenesis, Site-Directed KW - Calcium -- metabolism KW - Cattle KW - Isoenzymes -- deficiency KW - Phosphorylation KW - Transfection KW - Molecular Sequence Data KW - Diglycerides -- physiology KW - Nuclear Proteins -- metabolism KW - Cell Line KW - DNA-Binding Proteins -- metabolism KW - Receptors, Antigen, T-Cell -- metabolism KW - Signal Transduction -- genetics KW - Signal Transduction -- immunology KW - Tyrosine -- genetics KW - Tyrosine -- metabolism KW - Type C Phospholipases -- deficiency KW - Receptors, Antigen, B-Cell -- physiology KW - Type C Phospholipases -- genetics KW - Receptors, Antigen, T-Cell -- physiology KW - Type C Phospholipases -- metabolism KW - Receptors, Antigen, B-Cell -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67806210?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=A+new+tyrosine+phosphorylation+site+in+PLC+gamma+1%3A+the+role+of+tyrosine+775+in+immune+receptor+signaling.&rft.au=Serrano%2C+Carmen+J%3BGraham%2C+Laurie%3BDeBell%2C+Karen%3BRawat%2C+Rashmi%3BVeri%2C+Maria+Concetta%3BBonvini%2C+Ezio%3BRellahan%2C+Barbara+L%3BReischl%2C+Ilona+G&rft.aulast=Serrano&rft.aufirst=Carmen&rft.date=2005-05-15&rft.volume=174&rft.issue=10&rft.spage=6233&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Impact of chronic GVHD therapy on the development of squamous-cell cancers after hematopoietic stem-cell transplantation: an international case-control study. AN - 67790044; 15687239 AB - Previous studies of recipients of hematopoietic stem-cell transplants suggest that graft-versus-host disease (GVHD) and its therapy may increase the risk for solid cancers, particularly squamous-cell carcinomas (SCCs) of the buccal cavity and skin. However, the importance and magnitude of these associations are not well characterized. We conducted a case-control study of 183 patients with posttransplantation solid cancers (58 SCCs, 125 non-SCCs) and 501 matched control patients within a cohort of 24,011 patients who underwent hematopoietic stem-cell transplantation (HSCT) at 215 centers worldwide. Our results showed that chronic GVHD and its therapy were strongly related to the risk for SCC, whereas no increase in risk was found for non-SCCs. Major risk factors for the development of SCC were long duration of chronic GVHD therapy (P < .001); use of azathioprine, particularly when combined with cyclosporine and steroids (P < .001); and severe chronic GVHD (P = .004). Given that most patients who received prolonged immunosuppressive therapy and those with severe chronic GVHD were also treated with azathioprine, the independent effects of these factors could not be evaluated. Additional analyses determined that prolonged immunosuppressive therapy and azathioprine use were also significant risk factors for SCC of the skin and of the oral mucosa. These data provide further encouragement for strategies to prevent chronic GVHD and for the development of more effective and less carcinogenic treatment regimens for patients with moderate or severe chronic GVHD. Our results also suggest that clinical screening for SCC is appropriate among patients exposed to persistent chronic GVHD, prolonged immunosuppressive therapy, or both. JF - Blood AU - Curtis, Rochelle E AU - Metayer, Catherine AU - Rizzo, J Douglas AU - Socié, Gérard AU - Sobocinski, Kathleen A AU - Flowers, Mary E D AU - Travis, William D AU - Travis, Lois B AU - Horowitz, Mary M AU - Deeg, H Joachim AD - Radiation Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Executive Plaza South, Suite 7042, 6120 Executive Blvd, Bethesda, MD 20892, USA. rcurtis@mail.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 3802 EP - 3811 VL - 105 IS - 10 SN - 0006-4971, 0006-4971 KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Child KW - Internationality KW - Child, Preschool KW - Infant KW - Immunosuppression -- adverse effects KW - Risk Factors KW - Adult KW - Case-Control Studies KW - Middle Aged KW - Follow-Up Studies KW - Adolescent KW - Time Factors KW - Female KW - Male KW - Neoplasms, Squamous Cell -- pathology KW - Neoplasms, Squamous Cell -- therapy KW - Neoplasms, Squamous Cell -- immunology KW - Graft vs Host Disease -- immunology KW - Graft vs Host Disease -- therapy KW - Hematopoietic Stem Cell Transplantation KW - Neoplasms, Squamous Cell -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67790044?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Effect+of+topical+iodine+treatment+on+early+sulfur-mustard-induced+cutaneous+changes+and+epidermal-cell+proliferation&rft.au=Trivedi%2C+S%3BWormser%2C+U%3BFlagler%2C+N%3BBrodsky%2C+B%3BPeddada%2C+S%3BNyska%2C+A&rft.aulast=Trivedi&rft.aufirst=S&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Blood. 1999 Oct 1;94(7):2208-16 [10498590] Stat Med. 1999 Mar 30;18(6):695-706 [10204198] Ann Intern Med. 1999 Nov 16;131(10):738-44 [10577296] J Natl Cancer Inst. 1999 Dec 1;91(23):1992-3 [10580016] J Clin Oncol. 2000 Jan;18(2):348-57 [10637249] Blood. 2000 Jun 15;95(12):3702-9 [10845900] Lancet. 2000 May 27;355(9218):1886-7 [10866449] J Clin Oncol. 2001 Jan 15;19(2):464-71 [11208840] Blood. 2002 Jul 15;100(2):406-14 [12091329] Blood. 2002 Aug 1;100(3):761-7 [12130483] Blood Rev. 2002 Jun;16(2):135-46 [12127957] Nature. 2002 Dec 19-26;420(6917):860-7 [12490959] Blood. 2003 Feb 1;101(3):822-6 [12393424] J Clin Oncol. 2003 Apr 1;21(7):1352-8 [12663726] Transpl Int. 2003 Mar;16(3):146-53 [12664208] N Engl J Med. 2003 Apr 24;348(17):1681-91 [12711744] Cancer Metastasis Rev. 2003 Mar;22(1):95-102 [12716041] Br J Cancer. 2003 Oct 6;89(7):1221-7 [14520450] J Natl Cancer Inst. 2003 Dec 3;95(23):1772-83 [14652239] Lancet. 1984 Mar 17;1(8377):583-7 [6142304] Blood. 1988 Aug;72(2):546-54 [3042041] Cancer. 1990 Feb 1;65(3):473-6 [2297638] Blood. 1991 Jul 15;78(2):277-9 [2070065] Leuk Lymphoma. 1992 Aug;7(5-6):419-23 [1493443] N Engl J Med. 1993 Oct 14;329(16):1152-7 [8377778] J Clin Oncol. 1994 Oct;12(10):2187-92 [7931488] Transplantation. 1996 Mar 15;61(5):715-21 [8607173] Blood. 1996 Jan 1;87(1):386-92 [8547667] Lancet. 1995 Aug 12;346(8972):403-6 [7623570] N Engl J Med. 1997 Mar 27;336(13):897-904 [9070469] Clin Dermatol. 1997 May-Jun;15(3):427-37 [9255448] Blood. 1998 Mar 15;91(6):1833-44 [9490664] Lancet. 1998 Feb 28;351(9103):623-8 [9500317] J Am Acad Dermatol. 1999 Feb;40(2 Pt 1):177-86 [10025742] Nature. 1999 Feb 11;397(6719):530-4 [10028970] J Clin Oncol. 1999 Oct;17(10):3122-7 [10506608] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nitric oxide-induced resistance to hydrogen peroxide stress is a glutamate cysteine ligase activity-dependent process. AN - 67788397; 15855054 AB - Nitric oxide (*NO) is a reactive nitrogen species known to be involved in cytotoxic processes. Cells respond to cytotoxic injury by stress response induction leading to the development of cellular resistance. This report describes an *NO-induced stress response in Chinese hamster fibroblasts (HA1), which leads to glutathione synthesis-dependent resistance to H2O2-mediated oxidative stress. The development of resistance to H2O2 was completely abolished by the inhibition of glutamate cysteine ligase (GCL) during the first 8 h of recovery after *NO exposure. Altered thiol metabolism was observed immediately after *NO exposure as demonstrated by up to 75% decrease in intracellular thiol pools (glutathione, gamma-glutamylcysteine, and cysteine), which then reaccumulated during the *NO-mediated development of resistance. Immunoreactive protein and activity associated with GCL decreased immediately after exposure to *NO and then reaccumulated during the development of resistance to H2O2 challenge. Moreover, compared to N2 controls the activity levels of GCL in *NO-exposed cells increased approximately twofold 24 h after H2O2 challenge. These results demonstrate that *NO exposure is capable of inducing an adaptive response to H2O2-mediated oxidative stress in mammalian cells, which involves alterations in thiol metabolism and is dependent upon glutathione synthesis and increased GCL activity. JF - Free radical biology & medicine AU - Ridnour, Lisa A AU - Sim, Julia E AU - Choi, Jinah AU - Dickinson, Dale A AU - Forman, Henry J AU - Ahmad, Iman M AU - Coleman, Mitchell C AU - Hunt, Clayton R AU - Goswami, Prahbat C AU - Spitz, Douglas R AD - Division of Radiation and Cancer Biology, Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park Boulevard, Room 411, St. Louis, MO 63108, USA. ridnourl@mail.nih.gov Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1361 EP - 1371 VL - 38 IS - 10 SN - 0891-5849, 0891-5849 KW - Free Radical Scavengers KW - 0 KW - Oxidants KW - Sulfhydryl Compounds KW - Nitric Oxide KW - 31C4KY9ESH KW - Hydrogen Peroxide KW - BBX060AN9V KW - Glutamate-Cysteine Ligase KW - EC 6.3.2.2 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Oxidation-Reduction KW - Animals KW - Sulfhydryl Compounds -- metabolism KW - Cricetulus KW - Cells, Cultured KW - Glutathione -- metabolism KW - Cricetinae KW - Fibroblasts -- drug effects KW - Fibroblasts -- enzymology KW - Glutamate-Cysteine Ligase -- antagonists & inhibitors KW - Oxidants -- pharmacology KW - Oxidative Stress KW - Hydrogen Peroxide -- pharmacology KW - Nitric Oxide -- pharmacology KW - Fibroblasts -- cytology KW - Glutamate-Cysteine Ligase -- metabolism KW - Free Radical Scavengers -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67788397?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Nitric+oxide-induced+resistance+to+hydrogen+peroxide+stress+is+a+glutamate+cysteine+ligase+activity-dependent+process.&rft.au=Ridnour%2C+Lisa+A%3BSim%2C+Julia+E%3BChoi%2C+Jinah%3BDickinson%2C+Dale+A%3BForman%2C+Henry+J%3BAhmad%2C+Iman+M%3BColeman%2C+Mitchell+C%3BHunt%2C+Clayton+R%3BGoswami%2C+Prahbat+C%3BSpitz%2C+Douglas+R&rft.aulast=Ridnour&rft.aufirst=Lisa&rft.date=2005-05-15&rft.volume=38&rft.issue=10&rft.spage=1361&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microarray analysis of altered gene expression in murine fibroblasts transformed by nickel(II) to nickel(II)-resistant malignant phenotype AN - 19530216; 8249270 AB - B200 cells are Ni(II)-transformed mouse BALB/c-3T3 fibroblasts displaying a malignant phenotype and increased resistance to Ni(II) toxicity. In an attempt to find genes whose expression has been altered by the transformation, the Atlas Mouse Stress/Toxicology cDNA Expression Array (Clontech Laboratories, Inc., Palo Alto, CA) was used to analyze the levels of gene expression in both parental and Ni(II)-transformed cells. Comparison of the results revealed a significant up- or downregulation of the expression of 62 of the 588 genes present in the array (approximately 10.5%) in B200 cells. These genes were assigned to different functional groups, including transcription factors and oncogenes (9/14; fractions in parentheses denote the number of up-regulated versus the total number of genes assigned to this group), stress and DNA damage response genes (11 /12), growth factors and hormone receptors (6/9), metabolism (7/7), cell adhesion (2/7), cell cycle (3/6), apoptosis (3/4), and cell proliferation (2/3). Among those genes, overexpression of beta-catenin and its downstream targets c-myc and cyclin D1, together with upregulated cyclin G, points at the malignant character of B200 cells. While the increased expression of glutathione (GSH) synthetase, glutathione-S-transferase A4 (GSTA4), and glutathione-S-transferase theta (GSTT), together with high level of several genes responding to oxidative stress, suggests the enforcement of antioxidant defenses in Ni-transformed cells. JF - Toxicology and Applied Pharmacology AU - Kowara, R AU - Karaczyn, A AU - Cheng, RYS AU - Salnikow, K AU - Kasprzak, K S AD - National Cancer Institute at Frederick, Frederick, MD 21702, USA, Renata.Kowara@nrc-cnrc.gc.ca Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1 EP - 10 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 205 IS - 1 SN - 0041-008X, 0041-008X KW - Genetics Abstracts; Toxicology Abstracts; Biotechnology and Bioengineering Abstracts KW - Transformation KW - Apoptosis KW - Antioxidants KW - Glutathione KW - Cell cycle KW - Glutathione transferase KW - DNA microarrays KW - Hormones KW - Fibroblasts KW - Gene expression KW - Oncogenes KW - Oxidative stress KW - Growth factors KW - c-Myc protein KW - cyclin D1 KW - Toxicity KW - Cell adhesion KW - DNA damage KW - catenin KW - Atlases KW - Transcription factors KW - Cell proliferation KW - Metabolism KW - Cyclin G KW - W 30925:Genetic Engineering KW - X 24310:Pharmaceuticals KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19530216?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Microarray+analysis+of+altered+gene+expression+in+murine+fibroblasts+transformed+by+nickel%28II%29+to+nickel%28II%29-resistant+malignant+phenotype&rft.au=Kowara%2C+R%3BKaraczyn%2C+A%3BCheng%2C+RYS%3BSalnikow%2C+K%3BKasprzak%2C+K+S&rft.aulast=Kowara&rft.aufirst=R&rft.date=2005-05-15&rft.volume=205&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.10.006 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-07-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Transformation; Antioxidants; Apoptosis; Glutathione; Cell cycle; Toxicity; Glutathione transferase; Hormones; DNA microarrays; Fibroblasts; Cell adhesion; Gene expression; DNA damage; catenin; Oncogenes; Atlases; Oxidative stress; Transcription factors; Growth factors; Cell proliferation; Metabolism; c-Myc protein; cyclin D1; Cyclin G DO - http://dx.doi.org/10.1016/j.taap.2004.10.006 ER - TY - JOUR T1 - Invasion of Human Tissue Ex Vivo by Borrelia burgdorferi AN - 17505003; 6403552 AB - Borrelia burgdorferi sensu stricto is an etiological agent of Lyme disease. The lack of an adequate ex vivo system for human tissue infection is an obstacle to fully understanding the molecular mechanisms of invasion of tissue by B. burgdorferi and its adaptation within the human host. Here, we report on the development of such a system. We inoculated blocks of human tonsillar tissue with B. burgdorferi spirochetes, cultured them in a low-shear rotating wall vessel (RWV) bioreactor, and analyzed them using light and electron microscopy, nested polymerase chain reaction (PCR), and quantitative real-time PCR. Also, we evaluated the expression of the outer surface proteins (Osps) OspA and OspC by use of quantitative Western blotting. Light and electron microscopic analysis revealed multiple spirochetes localized extracellularly within the tissue, and their identity was confirmed by PCR. Quantification of spirochetes inside the RWV-cultured tonsillar tissue demonstrated that the number of B. burgdorferi exceeded the initial inoculum by an order of magnitude, indicating that spirochetes replicated in the tissue. Electron microscopic analysis showed that some spirochetes were arranged in cystic structures and that invading spirochetes differentially expressed surface proteins; both of these features have been described for infected tissues in vivo. The system we have developed can be used to study B. burgdorferi pathogenesis under controlled conditions ex vivo, in particular to explore the gene activation responsible for the adaptation of B. burgdorferi to human tissue that leads to Lyme disease. JF - Journal of Infectious Diseases AU - Duray, PH AU - Yin, S-R AU - Ito, Y AU - Bezrukov, L AU - Cox, C AU - Cho, M-S AU - Fitzgerald, W AU - Dorward, D AU - Zimmerberg, J AU - Margolis, L AD - Department of Pathology, National Cancer Institute, National Institutes of Health, and National Aeronautics and Space Administration/National Institutes of Health Center for Three-Dimensional Tissue Culture, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2005/05/15/ PY - 2005 DA - 2005 May 15 SP - 1747 EP - 1754 VL - 191 IS - 10 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology KW - Molecular modelling KW - Adaptations KW - outer surface proteins KW - Borrelia burgdorferi KW - Spirochetes KW - Tonsil KW - Bioreactors KW - Controlled conditions KW - Polymerase chain reaction KW - Electron microscopy KW - Transcription activation KW - Lyme disease KW - J 02855:Human Bacteriology: Others UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17505003?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Invasion+of+Human+Tissue+Ex+Vivo+by+Borrelia+burgdorferi&rft.au=Duray%2C+PH%3BYin%2C+S-R%3BIto%2C+Y%3BBezrukov%2C+L%3BCox%2C+C%3BCho%2C+M-S%3BFitzgerald%2C+W%3BDorward%2C+D%3BZimmerberg%2C+J%3BMargolis%2C+L&rft.aulast=Duray&rft.aufirst=PH&rft.date=2005-05-15&rft.volume=191&rft.issue=10&rft.spage=1747&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Borrelia burgdorferi; Spirochetes; Polymerase chain reaction; Lyme disease; Tonsil; Adaptations; Transcription activation; Molecular modelling; outer surface proteins; Bioreactors; Controlled conditions; Electron microscopy ER - TY - JOUR T1 - Protein kinase C-dependent regulation of NAG-1/placental bone morphogenic protein/MIC-1 expression in LNCaP prostate carcinoma cells. AN - 67804196; 15757899 AB - NAG-1 (nonsteroidal anti-inflammatory drug-activated gene), a member of the transforming growth factor beta superfamily, is involved in cellular processes such as inflammation, apoptosis/survival, and tumorigenesis and is regulated by p53, Sp1, and Egr-1. In the current study, the regulation of NAG-1 expression in LNCaP human prostate carcinoma cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. TPA treatment increased NAG-1 protein and mRNA levels in a time- and concentration-dependent manner as well as NF-kappa B binding/transcriptional activity in LNCaP cells. Pretreatment with protein kinase C (PKC) inhibitor blocked the TPA-induced increase in NAG-1 protein levels and NF-kappa B binding/transcriptional activity, whereas an inhibition of p38, JNK, MEK activity had no effect on TPA-induced NAG-1 levels and NF-kappa B transcriptional activity. Expression of constitutively active PKCs induced an increase in NF-kappa B transcriptional activity and NAG-1 protein levels in LNCaP cells. The expression of NF-kappa B p65 induced NAG-1 promoter activity, and chromatin immunoprecipitation assay for p65 showed that NF-kappa B binds the NAG-1 promoter in LNCaP cells. Inhibition of TPA-induced NAG-1 expression by NAG-1 short interfering RNA blocked TPA-induced apoptosis in LNCaP cells, suggesting induction of NAG-1 negatively affects LNCaP cell survival. These results demonstrate that NAG-1 expression is up-regulated by TPA in LNCaP cells through a PKC-dependent pathway involving the activation of NF-kappa B. JF - The Journal of biological chemistry AU - Shim, Minsub AU - Eling, Thomas E AD - Eicosanoids Biochemistry Section, Laboratory of Molecular Carcinogenesis, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/05/13/ PY - 2005 DA - 2005 May 13 SP - 18636 EP - 18642 VL - 280 IS - 19 SN - 0021-9258, 0021-9258 KW - Carcinogens KW - 0 KW - Cytokines KW - GDF15 protein, human KW - Growth Differentiation Factor 15 KW - NF-kappa B KW - Protein Isoforms KW - RNA, Messenger KW - RNA, Small Interfering KW - Luciferases KW - EC 1.13.12.- KW - Protein Kinase C KW - EC 2.7.11.13 KW - JNK Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - p38 Mitogen-Activated Protein Kinases KW - MAP Kinase Kinase Kinase 1 KW - EC 2.7.11.25 KW - MAP Kinase Kinase 4 KW - EC 2.7.12.2 KW - Mitogen-Activated Protein Kinase Kinases KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Index Medicus KW - Blotting, Northern KW - Apoptosis KW - Cell Nucleus -- metabolism KW - Humans KW - MAP Kinase Kinase Kinase 1 -- metabolism KW - Transcription, Genetic KW - RNA, Small Interfering -- metabolism KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Mitogen-Activated Protein Kinase Kinases -- metabolism KW - Cell Survival KW - Promoter Regions, Genetic KW - Chromatin Immunoprecipitation KW - Time Factors KW - Male KW - Dose-Response Relationship, Drug KW - Luciferases -- metabolism KW - Cell Line, Tumor KW - Inflammation KW - Blotting, Western KW - RNA, Messenger -- metabolism KW - Transfection KW - JNK Mitogen-Activated Protein Kinases -- metabolism KW - NF-kappa B -- metabolism KW - Protein Kinase C -- metabolism KW - Prostatic Neoplasms -- metabolism KW - Cytokines -- genetics KW - Cytokines -- biosynthesis KW - Protein Kinase C -- chemistry KW - Gene Expression Regulation KW - Protein Kinase C -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67804196?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Protein+kinase+C-dependent+regulation+of+NAG-1%2Fplacental+bone+morphogenic+protein%2FMIC-1+expression+in+LNCaP+prostate+carcinoma+cells.&rft.au=Shim%2C+Minsub%3BEling%2C+Thomas+E&rft.aulast=Shim&rft.aufirst=Minsub&rft.date=2005-05-13&rft.volume=280&rft.issue=19&rft.spage=18636&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-12 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene expression profiling identifies IL-13 receptor alpha 2 chain as a therapeutic target in prostate tumor cells overexpressing adrenomedullin. AN - 67530898; 15609296 AB - Human adrenomedullin (AM) is a 52 amino acid peptide, which shares homology with the calcitonin gene-related peptide. Overexpression of AM in the prostate carcinoma cell line PC-3 results in growth inhibition with a 20% (for human AM) and 35% (for rat AM) increase in doubling time compared to parental or mock-transfected cells. We demonstrate by gene expression profiling that AM overexpression results in the dysregulation of approximately 100 genes. Examples of such genes include many involved in the formation of the cytoskeleton, cell adhesion and the extracellular matrix, as well as regulators of the cell cycle and apoptosis, cytokines and transcription factors. Several genes related to cell growth arrest, such as GADD45, IGF-BP6 and RUNX-3, are upregulated by AM. Interestingly, interleukin-13 receptor alpha 2 (IL-13R alpha 2) transcripts were significantly increased in clones overexpressing AM, which was confirmed by semiquantitative RT-PCR analysis. In addition, PC-3 cells treated with AM showed an overexpression of IL-13R alpha 2, which was abolished when cells were preincubated with an anti-AM blocking antibody. When PC-3 cells overexpressing AM and the IL-13R alpha 2 were treated with the highly specific IL13-PE38 cytotoxin, which binds to this receptor, a concentration-dependent inhibition of protein synthesis was observed. The IC(50) (concentration of cytotoxin inhibiting protein synthesis by 50%) ranged from 1 to 4 ng/ml. This cytotoxicity was specific as it was neutralized by the excess of IL-13 and confirmed by clonogenic assays. This study describes a novel AM-induced mechanism of tumor sensitization through the upregulation of functional IL-13R alpha 2 chain, an ideal target for the highly specific recombinant chimeric cytotoxin IL13-PE38. JF - International journal of cancer AU - Gonzalez-Moreno, Oscar AU - Calvo, Alfonso AU - Joshi, Bharat H AU - Abasolo, Ibane AU - Leland, Pamela AU - Wang, Zhou AU - Montuenga, Luis AU - Puri, Raj K AU - Green, Jeffrey E AD - Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05/10/ PY - 2005 DA - 2005 May 10 SP - 870 EP - 878 VL - 114 IS - 6 SN - 0020-7136, 0020-7136 KW - IL13RA1 protein, human KW - 0 KW - Il13ra1 protein, rat KW - Interleukin-13 Receptor alpha1 Subunit KW - Peptides KW - Receptors, Interleukin KW - Receptors, Interleukin-13 KW - Adrenomedullin KW - 148498-78-6 KW - Index Medicus KW - Apoptosis KW - Tumor Cells, Cultured KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Up-Regulation KW - Reverse Transcriptase Polymerase Chain Reaction KW - Cell Cycle KW - Male KW - Prostatic Neoplasms -- pathology KW - Gene Expression Profiling KW - Receptors, Interleukin -- genetics KW - Peptides -- analysis KW - Prostatic Neoplasms -- genetics KW - Receptors, Interleukin -- physiology KW - Receptors, Interleukin -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67530898?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Relationship+of+treatment-related+decreases+in+serum+alkaline+phosphatase+activity+with+alterations+in+body+weight+for+rats+in+13-week+toxicity+studies&rft.au=Betz%2C+L%3BHarvey%2C+E%3BTravlos%2C+G&rft.aulast=Betz&rft.aufirst=L&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-16 N1 - Date created - 2005-03-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Different vanilloid agonists cause different patterns of calcium response in CHO cells heterologously expressing rat TRPV1. AN - 67724522; 15820503 AB - The vanilloid receptor subtype 1 (TRPV1 or VR1) is expressed in nociceptive primary afferents of the C-fiber 'pain' pathway and has attracted considerable attention as a therapeutic target. Here, using rat TRPV1 heterologously expressed in Chinese hamster ovary cells, we show that different agonists show different patterns of modulation of the intracellular Ca2+ concentration, monitored in individual cells by fura-2 Ca2+ imaging. We identified 5 parameters (potency, maximal response, latency of response, variability of latency of response among individual cells, and desensitization) which behaved differently for different compounds. The potencies of the compounds examined ranged from EC50 values of 80 pM to 9 microM. Peak levels of induced [Ca2+]i were observed either higher (RTX) or lower (anandamide) than for capsaicin. Significant latencies of response were observed for some (e.g. RTX) but not other derivatives, with great variation among individual cells in this latency. Marked desensitization after stimulation was detected in some cases (e.g. anandamide, capsaicin); for others, no desensitization was observed. We conclude that structurally diverse vanilloid agonists induce marked diversity in the patterns of Ca2+ response. This diversity of response may provide opportunities for pharmacological exploitation. JF - Life sciences AU - Tóth, Attila AU - Wang, Yun AU - Kedei, Noémi AU - Tran, Richard AU - Pearce, Larry V AU - Kang, Sang-Uk AU - Jin, Mi-Kyung AU - Choi, Hyun-Kyung AU - Lee, Jeewoo AU - Blumberg, Peter M AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bldg. 37, Room 4048, 37 Convent Dr., MSC 4255, Bethesda, MD 20892-4255, United States. Y1 - 2005/05/06/ PY - 2005 DA - 2005 May 06 SP - 2921 EP - 2932 VL - 76 IS - 25 SN - 0024-3205, 0024-3205 KW - Arachidonic Acids KW - 0 KW - Calcium Channel Blockers KW - Diterpenes KW - Endocannabinoids KW - Fluorescent Dyes KW - Ion Channels KW - Polyunsaturated Alkamides KW - TRPV Cation Channels KW - Trpv1 protein, rat KW - resiniferatoxin KW - A5O6P1UL4I KW - Capsaicin KW - S07O44R1ZM KW - Calcium KW - SY7Q814VUP KW - Fura-2 KW - TSN3DL106G KW - anandamide KW - UR5G69TJKH KW - Index Medicus KW - Rats KW - Animals KW - Cricetulus KW - Kinetics KW - CHO Cells KW - Cricetinae KW - Calcium -- metabolism KW - Calcium Channel Blockers -- metabolism KW - Capsaicin -- metabolism KW - Arachidonic Acids -- metabolism KW - Ion Channels -- agonists KW - Diterpenes -- metabolism KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67724522?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Life+sciences&rft.atitle=Different+vanilloid+agonists+cause+different+patterns+of+calcium+response+in+CHO+cells+heterologously+expressing+rat+TRPV1.&rft.au=T%C3%B3th%2C+Attila%3BWang%2C+Yun%3BKedei%2C+No%C3%A9mi%3BTran%2C+Richard%3BPearce%2C+Larry+V%3BKang%2C+Sang-Uk%3BJin%2C+Mi-Kyung%3BChoi%2C+Hyun-Kyung%3BLee%2C+Jeewoo%3BBlumberg%2C+Peter+M&rft.aulast=T%C3%B3th&rft.aufirst=Attila&rft.date=2005-05-06&rft.volume=76&rft.issue=25&rft.spage=2921&rft.isbn=&rft.btitle=&rft.title=Life+sciences&rft.issn=00243205&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The GB1 Amyloid Fibril: Recruitment of the Peripheral beta -Strands of the Domain Swapped Dimer into the Polymeric Interface AN - 19809314; 6391087 AB - Three-dimensional domain swapping has been evoked as a mechanism for oligomerization of proteins. Here, we show for the immunoglobulin-binding domain B1 of streptococcal protein G (GB1) that fibril formation is observed readily for variants that exist as domain-swapped dimers. No fibril was formed by a revertant that exhibits the stable wild-type GB1 fold or a mutant comprising a highly destabilized, fluctuating ensemble of conformers. Structural features of the GB1 amyloid fibril were characterized by cysteine disulfide cross-linking. Residues in the outer edge beta -strands of the domain-swapped dimer readily form intermolecular disulfide bonds prior to and during fibril formation. On the basis of these data, a structural model for the assembly of domain-swapped dimers into a polymeric structure of the GB1 fibril is proposed. JF - Journal of Molecular Biology AU - Louis, J M AU - Byeon, IJL AU - Baxa, U AU - Gronenborn, A M AD - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA, gronenborn@nih.gov Y1 - 2005/05/06/ PY - 2005 DA - 2005 May 06 SP - 687 EP - 698 VL - 348 IS - 3 SN - 0022-2836, 0022-2836 KW - Microbiology Abstracts B: Bacteriology KW - Streptococcus KW - Data processing KW - Recruitment KW - Oligomerization KW - Disulfide bonds KW - Models KW - Cysteine KW - Fibrillogenesis KW - streptococcal protein G KW - Revertants KW - Fibrils KW - Amyloid KW - J 02310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19809314?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Molecular+Biology&rft.atitle=The+GB1+Amyloid+Fibril%3A+Recruitment+of+the+Peripheral+beta+-Strands+of+the+Domain+Swapped+Dimer+into+the+Polymeric+Interface&rft.au=Louis%2C+J+M%3BByeon%2C+IJL%3BBaxa%2C+U%3BGronenborn%2C+A+M&rft.aulast=Louis&rft.aufirst=J&rft.date=2005-05-06&rft.volume=348&rft.issue=3&rft.spage=687&rft.isbn=&rft.btitle=&rft.title=Journal+of+Molecular+Biology&rft.issn=00222836&rft_id=info:doi/10.1016%2Fj.jmb.2005.02.071 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Data processing; Cysteine; streptococcal protein G; Fibrillogenesis; Oligomerization; Recruitment; Disulfide bonds; Revertants; Fibrils; Models; Amyloid; Streptococcus DO - http://dx.doi.org/10.1016/j.jmb.2005.02.071 ER - TY - JOUR T1 - Qualitative assessment of IC50 values of inhibitors of the neuronal nicotinic acetylcholine receptor using a single chromatographic experiment and multivariate cluster analysis. AN - 67564153; 15797535 AB - It has been widely demonstrated that affinity chromatography can be used to derive binding affinities, and that these affinities can be correlated to data obtained using standard techniques such as membrane binding, ultrafiltration and equilibrium dialysis. The purpose of this study is to evaluate the use of immobilized nicotinic acetylcholine receptor stationary phase in chromatographic experiments to assess the functional activity of series of noncompetitive inhibitors (NCIs) as reflected in their IC50 values. Chromatographically determined retention values and computer generated molecular descriptors were obtained for 29 compounds and the data were analyzed by cluster analysis. The approach qualitatively ranked the test compounds as efficient NCIs (low IC50 values) or poor NCIs (high IC50 values). The data obtained with the 29 compounds used in this study demonstrate that the experimental approach had been able to place 25 of these compounds in the correct IC(50) clusters. To our knowledge, this is the first relationship established between chromatographic retention and IC50 for membrane-bound receptors. These results suggest that the chromatographic approach may be useful in development of lead drug candidates including the determination of off-target binding. JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences AU - Jozwiak, Krzysztof AU - Moaddel, Ruin AU - Yamaguchi, Rika AU - Ravichandran, Sarangan AU - Collins, Jack R AU - Wainer, Irving W AD - Gerontology Research Center, National institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. krzysztof.jozwiak@am.lublin.pl Y1 - 2005/05/05/ PY - 2005 DA - 2005 May 05 SP - 169 EP - 174 VL - 819 IS - 1 SN - 1570-0232, 1570-0232 KW - Nicotinic Antagonists KW - 0 KW - Receptors, Nicotinic KW - Index Medicus KW - Thermodynamics KW - Cluster Analysis KW - Multivariate Analysis KW - Nicotinic Antagonists -- pharmacology KW - Inhibitory Concentration 50 KW - Nicotinic Antagonists -- analysis KW - Chromatography, Affinity -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67564153?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+chromatography.+B%2C+Analytical+technologies+in+the+biomedical+and+life+sciences&rft.atitle=Qualitative+assessment+of+IC50+values+of+inhibitors+of+the+neuronal+nicotinic+acetylcholine+receptor+using+a+single+chromatographic+experiment+and+multivariate+cluster+analysis.&rft.au=Jozwiak%2C+Krzysztof%3BMoaddel%2C+Ruin%3BYamaguchi%2C+Rika%3BRavichandran%2C+Sarangan%3BCollins%2C+Jack+R%3BWainer%2C+Irving+W&rft.aulast=Jozwiak&rft.aufirst=Krzysztof&rft.date=2005-05-05&rft.volume=819&rft.issue=1&rft.spage=169&rft.isbn=&rft.btitle=&rft.title=Journal+of+chromatography.+B%2C+Analytical+technologies+in+the+biomedical+and+life+sciences&rft.issn=15700232&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-03-30 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The anti-apoptotic, glucocorticoid receptor cochaperone protein BAG-1 is a long-term target for the actions of mood stabilizers. AN - 67798275; 15872096 AB - Increasing data suggest that impairments of cellular plasticity/resilience underlie the pathophysiology of bipolar disorder. A series of microarray studies with validating criteria have recently revealed a common, novel target for the long-term actions of the structurally highly dissimilar mood stabilizers lithium and valproate: BAG-1 [BCL-2 (B-cell CLL/lymphoma 2)-associated athanogene]. Because BAG-1 attenuates glucocorticoid receptor (GR) nuclear translocation, activates ERK (extracellular signal-regulated kinase) MAP (mitogen-activated protein) kinases, and potentiates anti-apoptotic functions of BCL-2, extensive additional studies were undertaken. Chronic administration of both agents at therapeutic doses increased the expression of BAG-1 in rat hippocampus. Furthermore, these findings were validated at the protein level, and the effects were seen in a time frame consistent with therapeutic effects and were specific for mood stabilizers. Functional studies showed that either lithium or valproate, at therapeutically relevant levels, inhibited dexamethasone-induced GR nuclear translocation and inhibited GR transcriptional activity. Furthermore, small interfering RNA studies showed that these inhibitory effects on GR activity were mediated, at least in part, through BAG-1. The observation that BAG-1 inhibits glucocorticoid activation suggests that mood stabilizers may counteract the deleterious effects of hypercortisolemia seen in bipolar disorder by upregulating BAG-1. Additionally, these studies suggest that regulation of GR-mediated plasticity may play a role in the treatment of bipolar disorder and raise the possibility that agents affecting BAG-1 more directly may represent novel therapies for this devastating illness. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Zhou, Rulun AU - Gray, Neil A AU - Yuan, Peixiong AU - Li, Xiaoxia AU - Chen, Jingshan AU - Chen, Guang AU - Damschroder-Williams, Patricia AU - Du, Jing AU - Zhang, Lei AU - Manji, Husseini K AD - Laboratory of Molecular Pathophysiology, National Institute of Mental Health, Bethesda, Maryland 20852, USA. Y1 - 2005/05/04/ PY - 2005 DA - 2005 May 04 SP - 4493 EP - 4502 VL - 25 IS - 18 KW - Antimanic Agents KW - 0 KW - BCL2-associated athanogene 1 protein KW - DNA-Binding Proteins KW - Indoles KW - RNA, Small Interfering KW - Receptors, Glucocorticoid KW - Transcription Factors KW - DAPI KW - 47165-04-8 KW - Valproic Acid KW - 614OI1Z5WI KW - Dexamethasone KW - 7S5I7G3JQL KW - Lithium KW - 9FN79X2M3F KW - Alkaline Phosphatase KW - EC 3.1.3.1 KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Dexamethasone -- pharmacology KW - Humans KW - Alkaline Phosphatase -- metabolism KW - Blotting, Western -- methods KW - Cell Line, Tumor KW - Immunohistochemistry -- methods KW - Alkaline Phosphatase -- genetics KW - Receptors, Glucocorticoid -- metabolism KW - Neuroblastoma KW - Molecular Weight KW - Behavior, Animal KW - Rats KW - Transfection -- methods KW - Rats, Wistar KW - RNA, Small Interfering -- pharmacology KW - Indoles -- metabolism KW - Time Factors KW - Male KW - Valproic Acid -- pharmacology KW - Antimanic Agents -- pharmacology KW - Transcription Factors -- metabolism KW - Hippocampus -- metabolism KW - DNA-Binding Proteins -- genetics KW - Transcription Factors -- genetics KW - Lithium -- pharmacology KW - Hippocampus -- drug effects KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67798275?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=The+anti-apoptotic%2C+glucocorticoid+receptor+cochaperone+protein+BAG-1+is+a+long-term+target+for+the+actions+of+mood+stabilizers.&rft.au=Zhou%2C+Rulun%3BGray%2C+Neil+A%3BYuan%2C+Peixiong%3BLi%2C+Xiaoxia%3BChen%2C+Jingshan%3BChen%2C+Guang%3BDamschroder-Williams%2C+Patricia%3BDu%2C+Jing%3BZhang%2C+Lei%3BManji%2C+Husseini+K&rft.aulast=Zhou&rft.aufirst=Rulun&rft.date=2005-05-04&rft.volume=25&rft.issue=18&rft.spage=4493&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-23 N1 - Date created - 2005-05-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Functionally distinct polymorphic sequences in the human genome that are targets for p53 transactivation. AN - 67800075; 15843459 AB - The p53 tumor suppressor protein is a master regulatory transcription factor that coordinates cellular responses to DNA damage and cellular stress. Besides mutations in p53, or in proteins involved in the p53 response pathway, genetic variation in promoter response elements (REs) of p53 target genes is expected to alter biological responses to stress. To identify SNPs in p53 REs that may modify p53-controlled gene expression, we developed an approach that combines a custom bioinformatics search to identify candidate SNPs with functional yeast and mammalian cell assays to assess their effect on p53 transactivation. Among approximately 2 million human SNPs, we identified >200 that seem to disrupt functional p53 REs. Eight of these SNPs were evaluated in functional assays to determine both the activity of the putative RE and the impact of the candidate SNPs on transactivation. All eight candidate REs were functional, and in every case the SNP pair exhibited differential transactivation capacities. Additionally, six of the eight genes adjacent to these SNPs are induced by genotoxic stress or are activated directly by transfection with p53 cDNA. Thus, this strategy efficiently identifies SNPs that may differentially affect gene expression responses in the p53 regulatory pathway. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Tomso, Daniel J AU - Inga, Alberto AU - Menendez, Daniel AU - Pittman, Gary S AU - Campbell, Michelle R AU - Storici, Francesca AU - Bell, Douglas A AU - Resnick, Michael A AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Y1 - 2005/05/03/ PY - 2005 DA - 2005 May 03 SP - 6431 EP - 6436 VL - 102 IS - 18 SN - 0027-8424, 0027-8424 KW - DNA, Complementary KW - 0 KW - Tumor Suppressor Protein p53 KW - Luciferases KW - EC 1.13.12.- KW - Index Medicus KW - Yeasts KW - DNA, Complementary -- genetics KW - Plasmids -- genetics KW - Humans KW - Cell Line, Tumor KW - Computational Biology KW - Reverse Transcriptase Polymerase Chain Reaction KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Sequence Alignment KW - Transfection KW - Promoter Regions, Genetic -- genetics KW - Response Elements -- genetics KW - Polymorphism, Single Nucleotide -- genetics KW - Genome, Human KW - Transcriptional Activation -- genetics KW - Gene Expression Regulation KW - Tumor Suppressor Protein p53 -- genetics KW - Tumor Suppressor Protein p53 -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67800075?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.atitle=Functionally+distinct+polymorphic+sequences+in+the+human+genome+that+are+targets+for+p53+transactivation.&rft.au=Tomso%2C+Daniel+J%3BInga%2C+Alberto%3BMenendez%2C+Daniel%3BPittman%2C+Gary+S%3BCampbell%2C+Michelle+R%3BStorici%2C+Francesca%3BBell%2C+Douglas+A%3BResnick%2C+Michael+A&rft.aulast=Tomso&rft.aufirst=Daniel&rft.date=2005-05-03&rft.volume=102&rft.issue=18&rft.spage=6431&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Cell. 2000 Sep 15;102(6):849-62 [11030628] Nucleic Acids Res. 2001 Jan 1;29(1):308-11 [11125122] Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3416-21 [11248093] Nat Genet. 2001 Apr;27(4):371-2 [11279516] Arthritis Rheum. 2001 Aug;44(8):1782-5 [11508429] Mol Cell. 2001 Jul;8(1):57-69 [11511360] Biochemistry. 2002 May 28;41(21):6714-22 [12022875] Hum Mutat. 2002 Jun;19(6):607-14 [12007217] Annu Rev Biochem. 2002;71:817-46 [12045112] Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8467-72 [12077306] Genes Dev. 1999 Nov 15;13(22):3027-33 [10580009] Genes Dev. 2000 Apr 15;14(8):981-93 [10783169] Oncogene. 2002 Nov 7;21(51):7901-11 [12420228] Mol Cell Biol. 2002 Dec;22(24):8612-25 [12446780] J Immunol. 2003 Jan 1;170(1):132-8 [12496392] Nat Genet. 2003 Apr;33(4):457-8 [12652301] Nat Genet. 2003 Apr;33(4):439-40 [12665861] Cancer Lett. 2003 Apr 10;193(1):99-107 [12691829] Environ Health Perspect. 2003 Jun;111(8):1055-64 [12826477] Gene. 2003 Jul 17;312:207-13 [12909357] Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9934-9 [12909720] Mol Cell. 2003 Oct;12(4):1015-27 [14580351] Science. 2003 Nov 7;302(5647):1041-3 [14605368] Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9 [14630945] Nucleic Acids Res. 2004 Jan 1;32(Database issue):D528-32 [14681474] Physiol Genomics. 2004 Jan 15;16(2):184-93 [14583597] Cell. 2004 Feb 20;116(4):499-509 [14980218] Science. 2004 Mar 5;303(5663):1526-9 [14976262] Cancer Res. 2004 May 15;64(10):3361-4 [15150084] Science. 1992 May 8;256(5058):827-30 [1589764] Nat Genet. 1992 Apr;1(1):45-9 [1301998] J Natl Cancer Inst. 1993 Jul 21;85(14):1159-64 [8320745] Hum Mol Genet. 1994 Sep;3(9):1537-42 [7833908] Genes Dev. 1996 May 1;10(9):1054-72 [8654922] Brain Res Dev Brain Res. 1999 Jun 2;115(2):183-93 [10407135] Cell. 2004 Nov 24;119(5):591-602 [15550242] Nature. 2000 Nov 16;408(6810):307-10 [11099028] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of a UDP-glucuronosyltransferase regulates substrate specificity. AN - 67798628; 15845768 AB - UDP-glucuronosyltransferase (UGT) isozymes catalyze detoxification of numerous chemical toxins present in our daily diet and environment by conjugation to glucuronic acid. The special properties and enzymatic mechanism(s) that enable endoplasmic reticulum-bound UGT isozymes to convert innumerable structurally diverse lipophiles to excretable glucuronides are unknown. Inhibition of cellular UGT1A7 and UGT1A10 activities and of [33P]orthophosphate incorporation into immunoprecipitable proteins after exposure to curcumin or calphostin-C indicated that the isozymes are phosphorylated. Furthermore, inhibition of UGT phosphorylation and activity by treatment with PKCepsilon-specific inhibitor peptide supported PKC involvement. Co-immunoprecipitation, colocalization by means of immunofluorescence, and cross-linking studies of PKCepsilon and UGT1A7His revealed that the proteins reside within 11.4 angstroms of each other. Moreover, mutation of three PKC sites in each UGT isozyme demonstrated that T73A/G and T202A/G caused null activity, whereas S432G-UGT1A7 caused a major shift of its pH-8.5 optimum to 6.4 with new substrate selections, including 17beta-estradiol. S432G-UGT1A10 exhibited a minor pH shift without substrate alterations. PKCepsilon involvement was confirmed by the demonstration that PKCepsilon overexpression enhanced activity of UGT1A7 but not of its S432 mutant and the conversion of 17beta-[14C]estradiol by S432G-UGT1A7 but not by UGT1A7. Consistent with these observations, treatment of UGT1A7-transfected cells with PKCepsilon-specific inhibitor peptide or general PKC inhibitors increased 17beta-estradiol catalysis between 5- and 11-fold, with parallel decreases in phosphoserine-432. Here, we report a mechanism involving PKC-mediated phosphorylation of UGT such that phosphoserine/threonine regulates substrate specificity in response to chemical exposures, which possibly confers survival benefit. JF - Proceedings of the National Academy of Sciences of the United States of America AU - Basu, Nikhil K AU - Kovarova, Martina AU - Garza, Amanda AU - Kubota, Shigeki AU - Saha, Tapas AU - Mitra, Partha S AU - Banerjee, Rajat AU - Rivera, Juan AU - Owens, Ida S AD - Heritable Disorders Branch, National Institute of Child Health and Human Development, National Institutes of Health, Building 10, Room 9S-241, Bethesda, MD 20892-1830, USA. Y1 - 2005/05/03/ PY - 2005 DA - 2005 May 03 SP - 6285 EP - 6290 VL - 102 IS - 18 SN - 0027-8424, 0027-8424 KW - Carbon Radioisotopes KW - 0 KW - Isoenzymes KW - Naphthalenes KW - Phosphoserine KW - 17885-08-4 KW - Threonine KW - 2ZD004190S KW - Estradiol KW - 4TI98Z838E KW - Glucuronosyltransferase KW - EC 2.4.1.17 KW - UDP-glucuronosyltransferase, UGT1A7 KW - Protein Kinase C KW - EC 2.7.11.13 KW - calphostin C KW - I271P23G24 KW - Curcumin KW - IT942ZTH98 KW - Index Medicus KW - Animals KW - Threonine -- metabolism KW - COS Cells KW - Humans KW - Hydrogen-Ion Concentration KW - Immunoprecipitation KW - Cell Line, Tumor KW - Phosphoserine -- metabolism KW - Mutagenesis KW - Estradiol -- metabolism KW - Naphthalenes -- metabolism KW - Blotting, Western KW - Curcumin -- metabolism KW - Phosphorylation KW - Transfection KW - Cercopithecus aethiops KW - Mutation -- genetics KW - Substrate Specificity KW - Fluorescent Antibody Technique KW - Protein Kinase C -- metabolism KW - Protein Kinase C -- antagonists & inhibitors KW - Glucuronosyltransferase -- genetics KW - Glucuronosyltransferase -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67798628?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Protocol+optimization+for+the+evaluation+of+in+vitro+cytotoxicity+assays+for+estimating+rodent+and+human+acute+systemic+toxicity&rft.au=Paris%2C+M%3BStrickland%2C+J%3BStokes%2C+W%3BCasati%2C+S%3BTice%2C+R%3BRaabe%2C+H%3BCao%2C+C%3BClothier%2C+R%3BHaseman%2C+J%3BCrockett%2C+P&rft.aulast=Paris&rft.aufirst=M&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Biochem Biophys Res Commun. 2001 Jul 27;285(4):997-1006 [11467851] Biochem Biophys Res Commun. 1999 Jun 24;260(1):199-202 [10381366] Biochem Biophys Res Commun. 2003 Mar 28;303(1):98-104 [12646172] Cancer Res. 2003 Aug 15;63(16):5118-25 [12941843] J Biol Chem. 2004 Jan 9;279(2):1429-41 [14557274] Drug Metab Dispos. 2004 Jul;32(7):768-73 [15205394] J Biol Chem. 2004 Jul 2;279(27):28320-9 [15117964] J Biol Chem. 1972 Apr 25;247(8):2558-65 [4336378] Hepatology. 1989 Aug;10(2):163-7 [2501210] J Biol Chem. 1991 Jan 15;266(2):1043-7 [1898728] J Clin Invest. 1992 Jul;90(1):150-5 [1634606] J Clin Invest. 1992 Sep;90(3):799-809 [1522235] Carcinogenesis. 1993 May;14(5):857-61 [8504477] J Biol Chem. 1994 Jun 17;269(24):16862-6 [8207009] J Biol Chem. 1995 Feb 17;270(7):3284-91 [7852413] Biochemistry. 2000 Mar 7;39(9):2269-75 [10694393] FASEB J. 2001 Jul;15(9):1613-5 [11427503] Cancer Res. 1995 Mar 1;55(5):1045-51 [7866987] Science. 1995 Apr 14;268(5208):247-51 [7716516] Biochemistry. 1996 Aug 6;35(31):10119-24 [8756475] J Biol Chem. 1997 Jan 17;272(3):1417-20 [8999804] J Immunol. 1997 Sep 15;159(6):2624-32 [9300681] Exp Cell Res. 1998 Jul 10;242(1):294-302 [9665827] Cell Growth Differ. 1999 Mar;10(3):183-91 [10099832] Eur J Biochem. 1999 Mar;260(3):785-93 [10103008] Biochem J. 2002 May 1;363(Pt 3):537-45 [11964154] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Suppression of gross chromosomal rearrangements by the multiple functions of the Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae. AN - 67712680; 15811632 AB - The Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae has roles in the intra-S checkpoint, homologous recombination, non-homologous end joining, meiotic recombination, telomere maintenance and the suppression of gross chromosomal rearrangements (GCRs). The discovery of mutations in the genes encoding the human homologues of two MRX subunits that underlie the chromosome fragility syndromes, Ataxia telangiectasia-like disorder and Nijmegen breakage syndrome suggest that the MRX complex also functions in suppression of GCRs in human cells. Previously, we demonstrated that the deletion mutations in each of the MRX genes increased the rate of GCRs up to 1000-fold compared to wild-type rates. However, it has not been clear which molecular function of the MRX complex is important for suppression of GCRs. Here, we present evidence that at least three different activities of the MRX complex are important for suppression of GCRs. These include the nuclease activity of Mre11, an activity related to MRX complex formation and another activity that has a close link with the telomere maintenance function of the MRX complex. An activity related to MRX complex formation is especially important for the suppression of translocation type of GCRs. However, the non-homologous end joining function of MRX complex does not appear to participate in the suppression of GCRs. JF - DNA repair AU - Smith, Stephanie AU - Gupta, Amitabha AU - Kolodner, Richard D AU - Myung, Kyungjae AD - Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892, USA. Y1 - 2005/05/02/ PY - 2005 DA - 2005 May 02 SP - 606 EP - 617 VL - 4 IS - 5 SN - 1568-7864, 1568-7864 KW - Saccharomyces cerevisiae Proteins KW - 0 KW - XRS2 protein, S cerevisiae KW - Endodeoxyribonucleases KW - EC 3.1.- KW - Exodeoxyribonucleases KW - MRE11 protein, S cerevisiae KW - Adenosine Triphosphatases KW - EC 3.6.1.- KW - RAD5 protein, S cerevisiae KW - DNA Helicases KW - EC 3.6.4.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Telomere -- genetics KW - Mutation -- genetics KW - Translocation, Genetic KW - Saccharomyces cerevisiae -- genetics KW - Saccharomyces cerevisiae Proteins -- metabolism KW - Saccharomyces cerevisiae -- metabolism KW - Saccharomyces cerevisiae Proteins -- genetics KW - Adenosine Triphosphatases -- metabolism KW - Gene Rearrangement KW - Endodeoxyribonucleases -- genetics KW - Endodeoxyribonucleases -- metabolism KW - Exodeoxyribonucleases -- genetics KW - Exodeoxyribonucleases -- metabolism KW - Adenosine Triphosphatases -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67712680?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=DNA+repair&rft.atitle=Suppression+of+gross+chromosomal+rearrangements+by+the+multiple+functions+of+the+Mre11-Rad50-Xrs2+complex+in+Saccharomyces+cerevisiae.&rft.au=Smith%2C+Stephanie%3BGupta%2C+Amitabha%3BKolodner%2C+Richard+D%3BMyung%2C+Kyungjae&rft.aulast=Smith&rft.aufirst=Stephanie&rft.date=2005-05-02&rft.volume=4&rft.issue=5&rft.spage=606&rft.isbn=&rft.btitle=&rft.title=DNA+repair&rft.issn=15687864&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-28 N1 - Date created - 2005-04-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of urethane-induced genotoxicity and cell proliferation in CYP2E1-null mice AN - 17503121; 6391163 AB - Urethane is a multi-site animal carcinogen and was classified as ''reasonably anticipated to be a human carcinogen.'' Urethane is a fermentation by-product and found at appreciable levels in alcoholic beverages and foods such as bread and cheese. Recent work in this laboratory demonstrated for the first time that CYP2E1 is the principal enzyme responsible for urethane metabolism. The current studies were undertaken to assess the relationships between CYP2E1-mediated metabolism and urethane-induced genotoxicity and cell proliferation as determined by induction of micronucleated erythrocytes (MN) and expression of Ki-67, respectively, using CYP2E1-null and wild-type mice. Urethane was administered at 0 (vehicle), 1, 10, or 100mg/kg/day (p.o.), 5 days/week for 6 weeks. A significant dose-dependent increase in MN was observed in wild-type mice; however, a slight increase was measured in the MN-polychromatic erythrocytes in CYP2E1-null mice treated with 100mg/kg. A significant increase in the expression of Ki-67 was detected in the livers and the lungs (terminal bronchioles, alveoli, and bronchi) of wild-type mice administered 100mg urethane /kg in comparison to controls. In contrast, CYP2E1-null mice administered this dose exhibited negligible alterations in Ki-67 expression in the livers and lungs compared to controls. Interestingly, while Ki-67 expression in the forestomach decreased in wild-type mice, it increased in CYP2E1-null mice. Subsequent comparative metabolism studies demonstrated that total urethane-derived radioactivity in the plasma, liver, and lung was significantly higher in CYP2E1-null versus wild-type mice and un-metabolized urethane constituted greater than 83% of the radioactivity in CYP2E1-null mice. Un-metabolized urethane was not detectable in the plasma, liver, and lung of wild-type mice. In conclusion, these data demonstrated that CYP2E1-mediated metabolism of urethane, presumably via epoxide formation, is necessary for the induction of genotoxicity, and cell proliferation in the liver and lung of wild-type mice. JF - Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis AU - Hoffler, U AU - Dixon, D AU - Peddada, S AU - Ghanayem, B I AD - Meharry Medical College, Nashville, TN, USA, ghanayem@niehs.nih.gov Y1 - 2005/05/02/ PY - 2005 DA - 2005 May 02 SP - 58 EP - 72 PB - Elsevier B.V. VL - 572 IS - 1-2 SN - 0027-5107, 0027-5107 KW - Toxicology Abstracts KW - Bread KW - Epoxides KW - Alcoholic beverages KW - Fermentation KW - Erythrocytes KW - Genotoxicity KW - Carcinogens KW - Cheese KW - Alveoli KW - Bronchus KW - Lung KW - Liver KW - Radioactivity KW - Cell proliferation KW - Manganese KW - urethane KW - Metabolism KW - X 24221:Toxicity testing UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17503121?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.atitle=Inhibition+of+urethane-induced+genotoxicity+and+cell+proliferation+in+CYP2E1-null+mice&rft.au=Hoffler%2C+U%3BDixon%2C+D%3BPeddada%2C+S%3BGhanayem%2C+B+I&rft.aulast=Hoffler&rft.aufirst=U&rft.date=2005-05-02&rft.volume=572&rft.issue=1-2&rft.spage=58&rft.isbn=&rft.btitle=&rft.title=Mutation+Research-Fundamental+and+Molecular+Mechanisms+of+Mutagenesis&rft.issn=00275107&rft_id=info:doi/10.1016%2Fj.mrfmmm.2004.12.005 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - urethane; Lung; Liver; Metabolism; Cell proliferation; Genotoxicity; Radioactivity; Erythrocytes; Carcinogens; Manganese; Alcoholic beverages; Bronchus; Epoxides; Alveoli; Fermentation; Bread; Cheese DO - http://dx.doi.org/10.1016/j.mrfmmm.2004.12.005 ER - TY - JOUR T1 - Task-dependent intracortical inhibition is impaired in focal hand dystonia. AN - 85385313; pmid-15641012 AB - We tested whether task-dependent modulation of inhibition within the motor cortex is impaired in patients with dystonia. Paired-pulse transcranial magnetic stimulation (TMS) at an interstimulus interval of 2 msec was used to measure the effect of two different tasks on short ISI intracortical inhibition (SICI) in dystonic and normal subjects. In two experiments, SICI of the fourth dorsal interosseus (4DIO) and abductor pollicis brevis (APB) muscles were measured before and at the end of the training task. In the first experiment, subjects performed a nonselective task consisting of abducting the thumb, where the APB acted as agonist and the 4DIO as synergist. In the second experiment, the function of the 4DIO was changed as the subjects were asked to consciously inhibit this muscle while abducting the thumb (selective task). Therefore, while the APB was activated in both tasks, the 4DIO was activated in the nonselective task but was in the inhibitory surround in the selective task. We found that performance of the selective but not the nonselective task resulted in increased SICI in the 4DIO of normal but not in dystonic subjects. We conclude that task-dependent SICI is disturbed in patients with dystonia.Copyright 2005 Movement Disorder Society. JF - Movement disorders : official journal of the Movement Disorder Society AU - Bütefisch, Cathrin M AU - Boroojerdi, Babak AU - Chen, Robert AU - Battaglia, Fortunato AU - Hallett, Mark AD - Human Motor Control Section, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland 20892-1428, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 545 EP - 551 VL - 20 IS - 5 SN - 0885-3185, 0885-3185 KW - Index Medicus KW - National Library of Medicine KW - Dystonic Disorders: diagnosis KW - *Dystonic Disorders: physiopathology KW - Dystonic Disorders: therapy KW - Electromyography KW - Feedback KW - Female KW - Functional Laterality: physiology KW - *Hand: physiopathology KW - Humans KW - Magnetics: instrumentation KW - Male KW - Middle Aged KW - *Neural Inhibition: physiology KW - *Neural Pathways: physiopathology KW - *Psychomotor Performance KW - Severity of Illness Index KW - Teaching: methods KW - Visual Perception UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85385313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.atitle=Task-dependent+intracortical+inhibition+is+impaired+in+focal+hand+dystonia.&rft.au=B%C3%BCtefisch%2C+Cathrin+M%3BBoroojerdi%2C+Babak%3BChen%2C+Robert%3BBattaglia%2C+Fortunato%3BHallett%2C+Mark&rft.aulast=B%C3%BCtefisch&rft.aufirst=Cathrin&rft.date=2005-05-01&rft.volume=20&rft.issue=5&rft.spage=545&rft.isbn=&rft.btitle=&rft.title=Movement+disorders+%3A+official+journal+of+the+Movement+Disorder+Society&rft.issn=08853185&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2012-03-15 N1 - SuppNotes - Cites: J Neurol Neurosurg Psychiatry. 1989 Sep;52(9):1043-9[2795073]; Cites: Neurology. 1989 Jan;39(1):85-9[2909917]; Cites: Proc Natl Acad Sci U S A. 1988 Mar;85(6):2003-7[3162322]; Cites: Mov Disord. 1988;3(1):61-9[3173365]; Cites: Neurology. 1988 Jul;38(7):1005-12[3386815]; Cites: J Neurol Neurosurg Psychiatry. 1985 Aug;48(8):782-7[4031930]; Cites: Brain. 1985 Sep;108 ( Pt 3):593-608[4041776]; Cites: Electroencephalogr Clin Neurophysiol. 1994 Apr;93(2):138-46[7512920]; Cites: Electroencephalogr Clin Neurophysiol. 1994 Aug;91(2):79-92[7519144]; Cites: Ann Neurol. 1995 Feb;37(2):181-8[7847860]; Cites: Brain. 1994 Aug;117 ( Pt 4):859-76[7922471]; Cites: J Physiol. 1993 Nov;471:501-19[8120818]; Cites: J Neurol Neurosurg Psychiatry. 1995 Nov;59(5):493-8[8530933]; Cites: Mov Disord. 1995 Sep;10(5):556-61[8552105]; Cites: Exp Brain Res. 1996 Apr;109(1):127-35[8740215]; Cites: Ann Neurol. 1996 Sep;40(3):367-78[8797526]; Cites: Electroencephalogr Clin Neurophysiol. 1996 Dec;101(6):478-82[9020819]; Cites: J Physiol. 1997 Feb 1;498 ( Pt 3):817-23[9051592]; Cites: Neurosci Lett. 1997 Feb 7;222(3):167-70[9148241]; Cites: J Neurol Neurosurg Psychiatry. 1985 Jul;48(7):650-7[4031909]; Cites: Neurology. 1997 Oct;49(4):1054-9[9339689]; Cites: Exp Brain Res. 1998 Feb;118(3):421-6[9497149]; Cites: Exp Brain Res. 1998 Mar;119(2):265-8[9535577]; Cites: Brain. 1998 Jul;121 ( Pt 7):1195-212[9679773]; Cites: Adv Neurol. 1998;78:11-8[9750898]; Cites: Clin Neurophysiol. 1999 Mar;110(3):550-5[10363778]; Cites: Exp Brain Res. 2002 Sep;146(1):86-94[12192582]; Cites: J Neurol Sci. 1992 Nov;113(1):85-90[1469459]; Cites: J Neurophysiol. 1991 Jun;65(6):1542-53[1875261]; Cites: Neurology. 1991 Sep;41(9):1449-56[1891097]; Cites: J Neurophysiol. 1989 Apr;61(4):747-58[2542471]; Cites: Brain. 1989 Jun;112 ( Pt 3):681-97[2731027] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - The effects of tones on speaking frequency and intensity ranges in Mandarin and Min dialects. AN - 85382418; pmid-15957789 AB - The differences of speaking frequency and intensity in different tonal dialects has not been widely investigated. The purposes of this study were (1) to compare the speaking frequency and speaking intensity ranges of Mandarin and Min and (2) to compare the speaking frequency and intensity ranges of Mandarin and Min to those of American English. The subjects were 80 normal Taiwanese adults divided into two dialect groups, Mandarin and Min. The speaking F0, the highest speaking F0, the lowest speaking F0, the maximum range of speaking F0, and the intensity counterpart were obtained from reading in their native dialects. Statistical analysis revealed that Min speakers had a significantly greater maximum range of speaking intensity and a smaller lowest speaking intensity than Mandarin speakers, which indicated tonal effects by speakers of the Min dialect. Moreover, Mandarin and Min speakers had a greater maximum range of speaking F0 and maximum range of speaking intensity than American English speakers. The data may provide an assessment tool for Mandarin speakers and Min speakers. JF - The Journal of the Acoustical Society of America AU - Chen, Sheng H AD - Department of Speech and Hearing Disorders and Sciences, National Taipei College of Nursing, #89, Nei-Chiang Street, Wanhua 108, Taipei, Taiwan. shchen@ntcn.edu.tw Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3225 EP - 3230 VL - 117 IS - 5 SN - 0001-4966, 0001-4966 KW - Index Medicus KW - National Library of Medicine KW - Adult KW - China KW - Female KW - Humans KW - *Language KW - Male KW - *Phonation: physiology KW - *Pitch Perception KW - *Speech: physiology KW - Speech Production Measurement KW - Time Factors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85382418?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+the+Acoustical+Society+of+America&rft.atitle=The+effects+of+tones+on+speaking+frequency+and+intensity+ranges+in+Mandarin+and+Min+dialects.&rft.au=Chen%2C+Sheng+H&rft.aulast=Chen&rft.aufirst=Sheng&rft.date=2005-05-01&rft.volume=117&rft.issue=5&rft.spage=3225&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+the+Acoustical+Society+of+America&rft.issn=00014966&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Investigation of the role of congenital cytomegalovirus infection in the etiology of enlarged vestibular aqueducts. AN - 85379421; pmid-15897416 AB - To determine whether congenital cytomegalovirus (CMV) infection is an etiologic factor in the pathogenesis of enlarged vestibular aqueducts (EVA).Two different cohort studies. Subjects The study population comprised 19 subjects with a history of congenital CMV infection and sensorineural hearing loss (cohort 1); 39 subjects with nonsyndromic EVA and their unaffected mothers (cohort 2); and 16 control subjects with EVA associated with Pendred syndrome and bi-allelic mutations of the SLC26A4 gene and their unaffected mothers.In cohort 1, we detected EVA in 0 of 19 subjects with congenital CMV infection and sensorineural hearing loss. In cohort 2, anti-CMV serologic profiles were consistent with possible congenital CMV infection in 10 (26%) of 39 subjects with nonsyndromic EVA and 6 (38%) of 16 control subjects with Pendred syndrome (P = .52). These seroprevalence rates are similar to those expected in the general population (40%).In spite of their auditory phenotypic similarities, congenital CMV infection is not a significant factor in the etiology of EVA. JF - Archives of otolaryngology--head & neck surgery AU - Pryor, Shannon P AU - Demmler, Gail J AU - Madeo, Anne C AU - Yang, Yandan AU - Zalewski, Chris K AU - Brewer, Carmen C AU - Butman, John A AU - Fowler, Karen B AU - Griffith, Andrew J AD - Hearing Section, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 388 EP - 392 VL - 131 IS - 5 SN - 0886-4470, 0886-4470 KW - National Library of Medicine KW - Adolescent KW - Audiometry KW - Child KW - Child, Preschool KW - Cohort Studies KW - *Cytomegalovirus Infections: complications KW - *Cytomegalovirus Infections: congenital KW - Cytomegalovirus Infections: genetics KW - Female KW - Hearing Loss, Sensorineural: virology KW - Humans KW - Infant KW - Magnetic Resonance Imaging KW - Male KW - Mutation KW - *Vestibular Aqueduct: virology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85379421?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+otolaryngology--head+%26+neck+surgery&rft.atitle=Investigation+of+the+role+of+congenital+cytomegalovirus+infection+in+the+etiology+of+enlarged+vestibular+aqueducts.&rft.au=Pryor%2C+Shannon+P%3BDemmler%2C+Gail+J%3BMadeo%2C+Anne+C%3BYang%2C+Yandan%3BZalewski%2C+Chris+K%3BBrewer%2C+Carmen+C%3BButman%2C+John+A%3BFowler%2C+Karen+B%3BGriffith%2C+Andrew+J&rft.aulast=Pryor&rft.aufirst=Shannon&rft.date=2005-05-01&rft.volume=131&rft.issue=5&rft.spage=388&rft.isbn=&rft.btitle=&rft.title=Archives+of+otolaryngology--head+%26+neck+surgery&rft.issn=08864470&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Neurobehavioral effects of harmful algal bloom (HAB) toxins: a critical review. AN - 85378910; pmid-15892909 AB - Human exposure to naturally occurring marine toxins has been associated with a range of neurobehavioral abnormalities. The toxins are produced by harmful algal blooms (HABs) and are typically contracted through seafood consumption. The primary target of many of the HAB toxins is the neurologic system, and the neurobehavioral symptoms associated with the HAB illnesses have influenced public health policy. The HAB-related illnesses most frequently linked to neuropsychological disturbance are Amnesic Shellfish Poisoning, Ciguatera Fish Poisoning, and Possible Estuarine Associated Syndrome, which is associated with exposure to the Pfiesteria piscicida organism. Although the neurophysiologic mechanisms underlying many of the HAB illnesses have been well delineated, the literature examining the neuropsychological impairments is unclear and needs to be defined. This review is intended to introduce an emerging area of study linking HAB illnesses with neuropsychological changes. JF - Journal of the International Neuropsychological Society : JINS AU - Friedman, Melissa A AU - Levin, Bonnie E AD - The NIEHS Marine and Freshwater Biomedical Sciences Center, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Florida, USA. melissafried@yahoo.com Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 331 EP - 338 VL - 11 IS - 3 SN - 1355-6177, 1355-6177 KW - Index Medicus KW - National Library of Medicine KW - Amnesia: diagnosis KW - *Amnesia: etiology KW - *Ciguatera Poisoning: complications KW - *Ciguatera Poisoning: etiology KW - Humans KW - Neuropsychological Tests KW - *Pfiesteria piscicida UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85378910?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+International+Neuropsychological+Society+%3A+JINS&rft.atitle=Neurobehavioral+effects+of+harmful+algal+bloom+%28HAB%29+toxins%3A+a+critical+review.&rft.au=Friedman%2C+Melissa+A%3BLevin%2C+Bonnie+E&rft.aulast=Friedman&rft.aufirst=Melissa&rft.date=2005-05-01&rft.volume=11&rft.issue=3&rft.spage=331&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+International+Neuropsychological+Society+%3A+JINS&rft.issn=13556177&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Effect of catechol-O-methyltransferase val158met genotype on attentional control. AN - 85307071; pmid-15901785 AB - The cingulate cortex is richly innervated by dopaminergic projections and plays a critical role in attentional control (AC). Evidence indicates that dopamine enhances the neurophysiological signal-to-noise ratio and that dopaminergic tone in the frontal cortex is critically dependent on catechol-O-methyltransferase (COMT). A functional polymorphism (val158met) in the COMT gene accounts for some of the individual variability in executive function mediated by the dorsolateral prefrontal cortex. We explored the effect of this genetic polymorphism on cingulate engagement during a novel AC task. We found that the COMT val158met polymorphism also affects the function of the cingulate during AC. Individuals homozygous for the high-activity valine ("val") allele show greater activity and poorer performance than val/methionine ("met") heterozygotes, who in turn show greater activity and poorer performance than individuals homozygous for the low-activity met allele, and these effects are most evident at the highest demand for AC. These results indicate that met allele load and presumably enhanced dopaminergic tone improve the "efficiency" of local circuit processing within the cingulate cortex and thereby its function during AC. JF - The Journal of Neuroscience AU - Blasi Giuseppe AU - Mattay, Venkata S AU - Bertolino Alessandro AU - Elvevåg Brita AU - Callicott, Joseph H AU - Das Saumitra AU - Kolachana Bhaskar S AU - Egan, Michael F AU - Goldberg, Terry E AU - Weinberger, Daniel R AD - Clinical Brain Disorders Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1379, USA. PY - 2005 SP - 5038 EP - 5045 VL - 25 IS - 20 SN - 0270-6474, 0270-6474 KW - Magnetic Resonance Imaging KW - Regression Analysis KW - Analysis of Variance KW - Humans KW - Prefrontal Cortex KW - Methionine KW - Genotype KW - Oxygen KW - Photic Stimulation KW - Brain Mapping KW - Comparative Study KW - Catechol O-Methyltransferase KW - Adult KW - Case-Control Studies KW - Neuropsychological Tests KW - Image Processing, Computer-Assisted KW - Attention KW - Male KW - Female KW - Reaction Time KW - Valine KW - Polymorphism, Genetic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85307071?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Neuroscience&rft.atitle=Effect+of+catechol-O-methyltransferase+val158met+genotype+on+attentional+control.&rft.au=Blasi+Giuseppe%3BMattay%2C+Venkata+S%3BBertolino+Alessandro%3BElvev%C3%A5g+Brita%3BCallicott%2C+Joseph+H%3BDas+Saumitra%3BKolachana+Bhaskar+S%3BEgan%2C+Michael+F%3BGoldberg%2C+Terry+E%3BWeinberger%2C+Daniel+R&rft.aulast=Blasi+Giuseppe&rft.aufirst=&rft.date=2005-05-01&rft.volume=25&rft.issue=20&rft.spage=5038&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - eng DB - ComDisDome N1 - Last updated - 2010-05-07 ER - TY - JOUR T1 - An HIV vaccine: as we build it, will they come? AN - 838989665; 3349250 JF - Health affairs AU - McCluskey, Margaret M AU - Alexander, Sarah B AU - Larkin, Brenda D AU - Murguia, Matthew AU - Wakefield, Steven AD - National Institutes of Health ; Fred Hutchinson Cancer Research Center ; NIH Office of Program Operations and Scientific Information Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 643 EP - 652 VL - 24 IS - 3 SN - 0278-2715, 0278-2715 KW - Sociology KW - World Health Organization KW - Prevention KW - Health care KW - Medical research KW - AIDS KW - Policy making KW - Medical treatment KW - HIV KW - Private sector KW - Public health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/838989665?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+affairs&rft.atitle=An+HIV+vaccine%3A+as+we+build+it%2C+will+they+come%3F&rft.au=McCluskey%2C+Margaret+M%3BAlexander%2C+Sarah+B%3BLarkin%2C+Brenda+D%3BMurguia%2C+Matthew%3BWakefield%2C+Steven&rft.aulast=McCluskey&rft.aufirst=Margaret&rft.date=2005-05-01&rft.volume=24&rft.issue=3&rft.spage=643&rft.isbn=&rft.btitle=&rft.title=Health+affairs&rft.issn=02782715&rft_id=info:doi/10.1377%2Fhlthaff.24.3.643 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 7890 5792 10484; 5703 3617 6220; 7886 10902; 482 3617 6220; 10449 5772; 9625 9628; 10208; 5775 13521; 10072; 13737 6772 9030 DO - http://dx.doi.org/10.1377/hlthaff.24.3.643 ER - TY - JOUR T1 - Two C-Methyl Derivatives of [ super(11)C]WAY-100635 - Effects of an Amido a-Methyl Group on Metabolism and Brain 5-HT sub(1A) Receptor Radioligand Behavior in Monkey* AN - 754566982; 13409466 AB - [carbonyl- super(11)C]N-(2-(1-(4-(2-methoxyphenyl)-piperazinyl)ethyl)- N-pyridinyl)cyclohexanecarboxamide ([carbonyl- super(11)C]WAY-1006 35) is an effective radioligand for imaging brain 5-HT sub(1A) receptors with positron emission tomography (PET). However, this radioligand has some drawbacks for deriving relative regional receptor densities, including rapid metabolism, which acts against accurate definition of an arterial input function for compartmental modeling, and very low nonspecific binding in brain, which detracts from the accuracy of modeling by a simplified reference tissue (cerebellum) approach. Here, in a search for a radioligand that overcomes these limitations, we investigated the effects of introducing a single methyl group at either of the carbon atoms alpha to the amide bond in [ super(11)C]WAY-100635. Ligands with a methyl group on the alpha carbon of the cyclohexyl group (SWAY) or the alpha carbon of the C sub(2)H sub(4) linker ((R,S)-JWAY) were synthesized and tested for binding affinity and intrinsic activity at 5-HT sub(1A) receptors. SWAY was labeled with carbon-11 (t sub(1/2) = 20.4 minutes; b super(+) = 99.8%) in its O-methyl group and (R,S)-JWAY in its carbonyl group. Each radioligand was evaluated by PET experiments in cynomolgus monkey. SWAY and (R,S)-JWAY were found to be high-affinity antagonists at 5-HT sub(1A) receptors. After injection of [ super(11)C]SWAY into monkey, radioactivity uptake in brain reached a maximum of 3% at 4.5 minutes and decreased to 0.7% at 72 minutes. However, over the time span of the experiment, radioactivity concentrations in 5-HT sub(1A) receptor-rich brain regions were only fractionally higher than in cerebellum. Radioactivity represented by parent radioligand in plasma was 39% at 45 minutes. After injection of [ super(11)C](R,S)-JWAY alone, radioactivity uptake in brain reached a maximum of 4.8% at 2.5 minutes and decreased to 1.2% at 90 minutes. At this time, radioactivity concentration in 5-HT sub(1A) receptor-rich brain regions was markedly greater than in cerebellum. In another PET experiment, the monkey was predosed with WAY-100635 before [ super(11)C](R,S)-JWAY injection. At 90 minutes after injection, the ratio of radioactivity in 5-HT sub(1A) receptor-rich regions to that in cerebellum was reduced to near unity. Radioactivity represented by parent radioligand in plasma was 12% at 45 minutes. [ super(11)C](R,S)- JWAY, but not [ super(11)C]SWAY, gives a sizeable 5-HT sub(1A) receptor-selective PET signal in monkey. The presence of a C-methyl group adjacent to the amide bond in SWAY or (R,S)-JWAY fails to counter metabolism. JF - Molecular Imaging and Biology AU - McCarron, Julie A AU - Marchais-Oberwinkler, Sandrine AU - Pike, Victor W AU - Tarkiainen, Jari AU - Halldin, Christer AU - Sovago, Judit AU - Gulyas, Balazs AU - Wikstroem, Hakan V AU - Farde, Lars AD - MRC Cyclotron Unit, Imperial College School of Medicine, Hammersmith Hospital, Ducane Road, London, W12 0NN, UK, mccarronj@intra.nimh.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 209 EP - 219 PB - Springer-Verlag, Tiergartenstrasse 17 Heidelberg 69121 Germany VL - 7 IS - 3 SN - 1536-1632, 1536-1632 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Cerebellum KW - Brain KW - Carbon cycle KW - Antagonists KW - Carbon KW - Serotonin S1 receptors KW - Receptor density KW - Positron emission tomography KW - Radioisotopes KW - Cynomolgus KW - Radioactivity KW - carbonyls KW - amides KW - Metabolism KW - W 30910:Imaging KW - N3 11150:General and miscellaneous topics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/754566982?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Imaging+and+Biology&rft.atitle=Two+C-Methyl+Derivatives+of+%5B+super%2811%29C%5DWAY-100635+-+Effects+of+an+Amido+a-Methyl+Group+on+Metabolism+and+Brain+5-HT+sub%281A%29+Receptor+Radioligand+Behavior+in+Monkey*&rft.au=McCarron%2C+Julie+A%3BMarchais-Oberwinkler%2C+Sandrine%3BPike%2C+Victor+W%3BTarkiainen%2C+Jari%3BHalldin%2C+Christer%3BSovago%2C+Judit%3BGulyas%2C+Balazs%3BWikstroem%2C+Hakan+V%3BFarde%2C+Lars&rft.aulast=McCarron&rft.aufirst=Julie&rft.date=2005-05-01&rft.volume=7&rft.issue=3&rft.spage=209&rft.isbn=&rft.btitle=&rft.title=Molecular+Imaging+and+Biology&rft.issn=15361632&rft_id=info:doi/10.1007%2Fs11307-005-4127-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-08-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Neuroimaging; Carbon cycle; Brain; Cerebellum; Antagonists; Carbon; Serotonin S1 receptors; Radioisotopes; Positron emission tomography; Receptor density; Radioactivity; amides; carbonyls; Metabolism; Cynomolgus DO - http://dx.doi.org/10.1007/s11307-005-4127-5 ER - TY - JOUR T1 - Characteristics of a human prostate stromal cell line related to its use in a stromal-epithelial coculture model for the study of cancer chemoprevention. AN - 68576604; 16153146 AB - An immortalized human prostate stromal cell line (PS30) was previously established using recombinant retrovirus encoding human papillomavirus 16 gene products. In this study, we further characterize this stromal cell line for its potential use in a stromal-epithelial coculture model for prostate cancer prevention. Using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and immunocytochemistry, we examined expression of androgen receptor (AR), vitamin D receptor (VDR), prostate-specific antigen (PSA), transforming growth factor-beta (TGF-beta), and insulin-like growth factors (IGF) families and their receptors, metalloproteinases (MMP) MMP-2 and MMP-9, as well as the cells' ability to respond to the synthetic androgen R1881. The PS30 stromal cells do not express PSA, confirming their stromal origin. They are positive for both AR messenger ribonucleic acid (mRNA) and protein; however, they do not respond to growth stimulation by the synthetic androgen R1881. The PS30 cells express mRNA for VDR, TGF-betas, IGFs and their receptors, as well as the MMPs. Moreover, they produce significant amounts of TGF-beta1, TGF-beta2, IGFBP-3, and MMP-2 proteins. Our observations confirm the use of PS30 for the study of stromal-epithelial interactions in the modulation of prostate carcinogenesis. JF - In vitro cellular & developmental biology. Animal AU - Diaw, Lena AU - Roth, Mark AU - Schwinn, Debra A AU - d'Alelio, Mary E AU - Green, Lisa J AU - Tangrea, Joseph A AD - SAIC-Frederick Inc., National Cancer Institute/Advanced Technology Center, Bethesda, Maryland, USA. diawl@mail.nih.gov PY - 2005 SP - 142 EP - 148 VL - 41 IS - 5-6 SN - 1071-2690, 1071-2690 KW - DNA Primers KW - 0 KW - Receptors, Androgen KW - Receptors, Calcitriol KW - Transforming Growth Factor beta KW - Metribolone KW - 2C323EGI97 KW - Prostate-Specific Antigen KW - EC 3.4.21.77 KW - Matrix Metalloproteinase 2 KW - EC 3.4.24.24 KW - Matrix Metalloproteinase 9 KW - EC 3.4.24.35 KW - Index Medicus KW - Humans KW - Reverse Transcriptase Polymerase Chain Reaction KW - Cell Proliferation KW - Matrix Metalloproteinase 2 -- metabolism KW - Matrix Metalloproteinase 9 -- metabolism KW - Prostate-Specific Antigen -- metabolism KW - Receptors, Androgen -- metabolism KW - Enzyme-Linked Immunosorbent Assay KW - Receptors, Calcitriol -- metabolism KW - Transforming Growth Factor beta -- metabolism KW - Immunohistochemistry KW - Male KW - Cell Line -- metabolism KW - Cell Line -- cytology KW - Prostatic Neoplasms -- prevention & control KW - Stromal Cells -- metabolism KW - Stromal Cells -- cytology KW - Prostate -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68576604?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=In+vitro+cellular+%26+developmental+biology.+Animal&rft.atitle=Characteristics+of+a+human+prostate+stromal+cell+line+related+to+its+use+in+a+stromal-epithelial+coculture+model+for+the+study+of+cancer+chemoprevention.&rft.au=Diaw%2C+Lena%3BRoth%2C+Mark%3BSchwinn%2C+Debra+A%3Bd%27Alelio%2C+Mary+E%3BGreen%2C+Lisa+J%3BTangrea%2C+Joseph+A&rft.aulast=Diaw&rft.aufirst=Lena&rft.date=2005-05-01&rft.volume=41&rft.issue=5-6&rft.spage=142&rft.isbn=&rft.btitle=&rft.title=In+vitro+cellular+%26+developmental+biology.+Animal&rft.issn=10712690&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-28 N1 - Date created - 2005-09-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cyclooxygenase-2 expression is associated with increased size in human sporadic colorectal adenomas. AN - 68572842; 16158946 AB - Cyclooxygenase-2 (COX-2) has been implicated in colorectal carcinogenesis but its role is not completely defined. The expression of COX-2 was evaluated in 68 paraffin-embedded sporadic colorectal adenomas by immunohistochemistry. Associations between COX-2 expression and the clinicopathological characteristics of the adenomas were studied by contingency tables and the Chi-square test. Cytoplasmic staining for COX-2 protein was present in epithelial cells of 62 out of the 68 adenomas. COX-2 expression was not associated with age or gender. Furthermore, no significant correlations were found between the expression of the protein and histology (tubular vs tubulovillous), localization (proximal vs distal) or morphology (sessil vs pedunculated) of the adenomas. Both stromal and epithelial COX-2 expressions were higher in larger (>4 mm) compared with smaller (< or =4 mm) adenomas (p =0. 037 and p=0. 024). These data support the hypothesis that the expression of COX-2 may occur as a general phenomenon in colorectal adenomas. A size-dependent increase of COX-2 expression might be involved in colorectal carcinogenesis. JF - Anticancer research AU - Pisano, Carmela AU - Ottaiano, Alessandro AU - Tatangelo, Fabiana AU - Di Bonito, Maurizio AU - Falanga, Marzia AU - Iaffaioli, Vincenzo Rosario AU - Botti, Gerardo AU - Pignata, Sandro AU - Acquaviva, Angela Maria AD - Medical Oncology B, National Cancer Institute, via Mariano Semmola, 80131, Naples, Italy. carmen_pisano@libero.it PY - 2005 SP - 2065 EP - 2068 VL - 25 IS - 3B SN - 0250-7005, 0250-7005 KW - Membrane Proteins KW - 0 KW - Cyclooxygenase 2 KW - EC 1.14.99.1 KW - PTGS2 protein, human KW - Prostaglandin-Endoperoxide Synthases KW - Index Medicus KW - Paraffin Embedding KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Male KW - Female KW - Adenoma -- enzymology KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- enzymology KW - Adenoma -- pathology KW - Prostaglandin-Endoperoxide Synthases -- biosynthesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68572842?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Anticancer+research&rft.atitle=Cyclooxygenase-2+expression+is+associated+with+increased+size+in+human+sporadic+colorectal+adenomas.&rft.au=Pisano%2C+Carmela%3BOttaiano%2C+Alessandro%3BTatangelo%2C+Fabiana%3BDi+Bonito%2C+Maurizio%3BFalanga%2C+Marzia%3BIaffaioli%2C+Vincenzo+Rosario%3BBotti%2C+Gerardo%3BPignata%2C+Sandro%3BAcquaviva%2C+Angela+Maria&rft.aulast=Pisano&rft.aufirst=Carmela&rft.date=2005-05-01&rft.volume=25&rft.issue=3B&rft.spage=2065&rft.isbn=&rft.btitle=&rft.title=Anticancer+research&rft.issn=02507005&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-11-02 N1 - Date created - 2005-09-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Alcohol use disorder and illicit drug use in admissions to general hospitals in the United States. AN - 68033442; 16019976 AB - This study estimated the prevalence and explored the management of illicit drug use, illicit drug use associated with alcohol use disorder (AUD), and AUD without reported illicit drug use in a national sample of 2040 admissions to general hospitals in the United States. Surveyed in 1994, admissions were diagnosed with past 12-month DSM-IV AUD according to the Alcohol Use Disorders and Associated Disabilities Interview Schedule. Information about drug use was also included in the interview. Entries in hospital records were used to operationalize management. Prevalence of chronic drug use in hospital admissions was 5%, 14% in 18-44-year-old admissions, and 31% in admissions with an AUD. In admissions with an AUD, 45% reported no drug use. Detection rates were 82% for admissions with comorbid AUD and chronic drug use (where detection of either problem was assessed); detection rates hovered around 50% in admissions with one or the other condition. Low rates of treatment and referral (33% and 42%, respectively) were observed in the comorbid group; rates were 13-17% in admissions with AUD alone or illicit drug use alone. Findings indicate the need for increased attention to drug use and to AUD with and without other drug use among general hospital admissions. JF - The American journal on addictions AU - Smothers, Barbara A AU - Yahr, Harold T AD - Division of Epidemiology and Prevention Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA. bs86h@nih.gov PY - 2005 SP - 256 EP - 267 VL - 14 IS - 3 SN - 1055-0496, 1055-0496 KW - Street Drugs KW - 0 KW - Index Medicus KW - Mass Screening KW - Medical Records KW - Length of Stay KW - Humans KW - Adult KW - Interviews as Topic KW - Hospitals, General KW - Adolescent KW - United States -- epidemiology KW - Male KW - Female KW - Prevalence KW - Alcohol-Related Disorders -- diagnosis KW - Substance-Related Disorders -- diagnosis KW - Patient Admission KW - Substance-Related Disorders -- epidemiology KW - Alcohol-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68033442?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+on+addictions&rft.atitle=Alcohol+use+disorder+and+illicit+drug+use+in+admissions+to+general+hospitals+in+the+United+States.&rft.au=Smothers%2C+Barbara+A%3BYahr%2C+Harold+T&rft.aulast=Smothers&rft.aufirst=Barbara&rft.date=2005-05-01&rft.volume=14&rft.issue=3&rft.spage=256&rft.isbn=&rft.btitle=&rft.title=The+American+journal+on+addictions&rft.issn=10550496&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-30 N1 - Date created - 2005-07-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Targeted disruption of p53 attenuates doxorubicin-induced cardiac toxicity in mice. AN - 68029615; 16013437 AB - Use of the chemotherapeutic agent doxorubicin (Dox) is limited by dose-dependent cardiotoxic effects. The molecular mechanism underlying these toxicities are incompletely understood, but previous results have demonstrated that Dox induces p53 expression. Because p53 is an important regulator of the cell birth and death we hypothesized that targeted disruption of the p53 gene would attenuate Dox-induced cardiotoxicity. To test this, female 6-8 wk old C57BL wild-type (WT) or p53 knockout (p53 KO) mice were randomized to either saline or Dox 20 mg/kg via intraperitoneal injection. Animals were serially imaged with high-frequency (14 MHz) two-dimensional echocardiography. Measurements of left ventricle (LV) systolic function as assessed by fractional shortening (FS) demonstrated a decline in WT mice as early as 4 days after Dox injection and by 2 wk demonstrated a reduction of 31 +/- 16% (P < 0.05) from the baseline. In contrast, in p53 KO mice, LV FS was unchanged over the 2 wk period following Dox injection. Apoptosis of cardiac myocytes as measured by the TUNEL and ligase reactions were significantly increased at 24 h after Dox treatment in WT mice but not in p53 KO mice. After Dox injection, levels of myocardial glutathione and Cu/Zn superoxide dismutase were preserved in p53 KO mice, but not in WT animals. These observations suggest that p53 mediated signals are likely to play a significant role in Dox-induced cardiac toxicity and that they may modulate Dox-induced oxidative stress. JF - Molecular and cellular biochemistry AU - Shizukuda, Yukitaka AU - Matoba, Satoaki AU - Mian, Omar Y AU - Nguyen, Tammy AU - Hwang, Paul M AD - Cardiovascular Branch, National Heart Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. shizukuy@nhlbi.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 25 EP - 32 VL - 273 IS - 1-2 SN - 0300-8177, 0300-8177 KW - Antibiotics, Antineoplastic KW - 0 KW - Cdkn1a protein, mouse KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - Reactive Oxygen Species KW - Tumor Suppressor Protein p53 KW - Doxorubicin KW - 80168379AG KW - Superoxide Dismutase KW - EC 1.15.1.1 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Apoptosis KW - Myocardium -- pathology KW - Glutathione -- metabolism KW - Superoxide Dismutase -- metabolism KW - Mice KW - Myocytes, Cardiac -- pathology KW - Myocardium -- metabolism KW - Cell Cycle Proteins -- metabolism KW - Mice, Knockout KW - In Situ Nick-End Labeling KW - Oxidative Stress KW - Mice, Inbred C57BL KW - Female KW - Male KW - Tumor Suppressor Protein p53 -- physiology KW - Cardiomyopathies -- metabolism KW - Cardiomyopathies -- prevention & control KW - Heart -- drug effects KW - Doxorubicin -- toxicity KW - Heart -- physiopathology KW - Antibiotics, Antineoplastic -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68029615?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biochemistry&rft.atitle=Targeted+disruption+of+p53+attenuates+doxorubicin-induced+cardiac+toxicity+in+mice.&rft.au=Shizukuda%2C+Yukitaka%3BMatoba%2C+Satoaki%3BMian%2C+Omar+Y%3BNguyen%2C+Tammy%3BHwang%2C+Paul+M&rft.aulast=Shizukuda&rft.aufirst=Yukitaka&rft.date=2005-05-01&rft.volume=273&rft.issue=1-2&rft.spage=25&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biochemistry&rft.issn=03008177&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-13 N1 - Date created - 2005-07-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Animal models for acquired bone marrow failure syndromes. AN - 68028036; 16012128 AB - Bone marrow failure is a disease characterized by a drastic decline in the marrow's functional ability to produce mature blood cells. Aplastic anemia, a disease in which patients have essentially empty bone marrow accompanied by severe anemia, neutropenia, and thrombocytopenia, presents a paradigm for bone marrow failure. Damage to the marrow may first result from exposure to toxic chemicals, drug overdose, radiation, and viral infection; however, it is the extended immune-mediated reaction that causes massive destruction of hematopoietic cells and leads to marrow hypoplasia and peripheral pancytopenia. In recent years, animal models of acquired bone marrow failure syndromes have helped to strengthen our understanding of the mechanisms causing bone marrow failure. In this review, animal models for bone marrow failure are summarized by two groups: 1) bone marrow failure induced by toxic chemicals and drugs such as benzene, busulfan, and chloramphenicol, and radiation, and 2) models developed by an immune-related mechanism such as viral infection or foreign lymphocyte infusion. JF - Clinical medicine & research AU - Chen, Jichun AD - Hematology Branch, NHLBI, NIH Building 10, Clinical Research Center, Room 3E-5132, Bethesda, MD 20892-1202, USA. chenji@nhlbi.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 102 EP - 108 VL - 3 IS - 2 SN - 1539-4182, 1539-4182 KW - Chloramphenicol KW - 66974FR9Q1 KW - Busulfan KW - G1LN9045DK KW - Benzene KW - J64922108F KW - Index Medicus KW - Animals KW - Anemia, Aplastic -- etiology KW - Virus Diseases -- complications KW - Syndrome KW - Humans KW - Chloramphenicol -- toxicity KW - Benzene -- toxicity KW - Disease Models, Animal KW - Radiation Injuries, Experimental -- etiology KW - Mice KW - Busulfan -- toxicity KW - Bone Marrow Diseases -- etiology KW - Bone Marrow Diseases -- chemically induced KW - Bone Marrow Diseases -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68028036?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+medicine+%26+research&rft.atitle=Animal+models+for+acquired+bone+marrow+failure+syndromes.&rft.au=Chen%2C+Jichun&rft.aulast=Chen&rft.aufirst=Jichun&rft.date=2005-05-01&rft.volume=3&rft.issue=2&rft.spage=102&rft.isbn=&rft.btitle=&rft.title=Clinical+medicine+%26+research&rft.issn=15394182&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-02 N1 - Date created - 2005-07-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Exp Pathol. 2002 Oct;83(5):225-38 [12641819] N Engl J Med. 2005 Apr 7;352(14):1413-24 [15814878] Cancer Res. 2003 Sep 1;63(17):5414-9 [14500376] Rev Med Virol. 2003 Nov-Dec;13(6):347-59 [14625883] N Engl J Med. 2004 Feb 5;350(6):586-97 [14762186] Lancet. 2004 Jul 24-30;364(9431):355-64 [15276395] Blood. 2004 Sep 15;104(6):1671-8 [15166031] Stem Cells. 2004;22(5):750-8 [15342939] Br J Haematol. 1967 Jul;13(4):482-91 [6029951] Blood. 1974 Jul;44(1):49-56 [4834516] Br J Cancer. 1975 Aug;32(2):193-8 [764844] Br J Haematol. 1976 Apr;32(4):525-31 [1259934] Blood. 1978 Apr;51(4):601-10 [305267] Exp Hematol. 1978 Nov;6(10):791-800 [33819] Int J Radiat Oncol Biol Phys. 1979 Sep;5(9):1621-5 [395140] J Lab Clin Med. 1980 Jul;96(1):36-46 [7391656] Cell Tissue Kinet. 1981 Jan;14(1):8590 [7471158] Exp Hematol. 1980 Jul;8(6):788-94 [7202582] Exp Hematol. 1982 Jan;10(1):20-5 [7060658] J Cell Physiol. 1982 Sep;112(3):345-52 [7130283] Exp Hematol. 1983 Jul;11(6):542-52 [6617789] Blut. 1984 May;48(5):277-84 [6722358] Int J Exp Pathol. 2003 Feb;84(1):31-48 [12694485] Food Chem Toxicol. 2000 Oct;38(10):925-38 [11039326] Food Chem Toxicol. 2001 Apr;39(4):375-83 [11295484] Toxicology. 2001 May 21;162(3):179-91 [11369114] Toxicol Appl Pharmacol. 2001 Jul 15;174(2):139-45 [11446829] Life Sci. 2001 Aug 10;69(12):1373-9 [11531161] J Clin Invest. 2001 Sep;108(5):765-73 [11544283] Ann Intern Med. 2002 Apr 2;136(7):534-46 [11926789] Food Chem Toxicol. 2002 Dec;40(12):1849-61 [12419700] Int J Cell Cloning. 1984 Jul;2(4):263-71 [6379064] Blood. 1984 Nov;64(5):1036-41 [6487805] Anticancer Res. 1985 Jan-Feb;5(1):101-10 [3888044] Int J Cell Cloning. 1986 Sep;4(5):357-67 [3772175] Exp Hematol. 1987 Mar;15(3):269-75 [3493173] J Exp Pathol. 1987;3(3):259-69 [2835466] Exp Hematol. 1989 May;17(4):335-9 [2468513] Toxicol Lett. 1991 Apr;56(1-2):159-66 [2017773] Blood. 1991 Aug 15;78(4):938-44 [1868253] Viral Immunol. 1991 Winter;4(4):269-80 [1668061] Exp Hematol. 1994 Jul;22(7):573-81 [8013573] Arch Toxicol. 1995;69(3):141-8 [7717869] Blood. 1995 Jun 1;85(11):3183-90 [7538820] J Toxicol Environ Health. 1995 Oct;46(2):183-201 [7563217] Br J Haematol. 1995 Sep;91(1):245-52 [7577642] Blood. 1996 May 15;87(10):4149-57 [8639773] Leuk Lymphoma. 1996 Apr;21(3-4):217-23 [8726402] J Virol. 1997 Jun;71(6):4589-98 [9151853] Hum Exp Toxicol. 1998 Jan;17(1):8-17 [9491332] J Exp Med. 1998 Jun 1;187(11):1903-20 [9607930] Exp Hematol. 1999 May;27(5):895-903 [10340406] Hum Exp Toxicol. 1999 Sep;18(9):566-76 [10523871] Exp Hematol. 2004 Dec;32(12):1163-72 [15588941] Leukemia. 2005 Feb;19(2):217-22 [15668701] Comment In: Clin Med Res. 2005 May;3(2):63-4 [16012122] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibitors of histone deacetylases alter kinetochore assembly by disrupting pericentromeric heterochromatin. AN - 67956052; 15846093 AB - The kinetochore, a multi-protein complex assembled on centromeric chromatin in mitosis, is essential for sister chromosome segregation. We show here that inhibition of histone deacetylation blocks mitotic progression at prometaphase in two human tumor cell lines by interfering with kinetochore assembly. Decreased amounts of hBUB1, CENP-F and the motor protein CENP-E were present on kinetochores of treated cells. These kinetochores failed to nucleate and inefficiently captured microtubules, resulting in activation of the mitotic checkpoint. Addition of histone deacetylase inhibitors prior to the end of S-phase resulted in decreased HP1-beta on pericentromeric heterochromatin in S-phase and G(2), decreased pericentromeric targeting of Aurora B kinase, resulting in decreased premitotic phosphorylation of pericentromeric histone H3(S10) in G(2), followed by assembly of deficient kinetochores in M-phase. HP1-beta, Aurora B and the affected kinetochore proteins all were present at normal levels in treated cells; thus, effects of the inhibitors on mitotic progression do not seem to reflect changes in gene expression. In vitro kinase activity of Aurora B isolated from treated cells was unaffected. We propose that the increased presence in pericentromeric heterochromatin of histone H3 acetylated at K9 is responsible for the mitotic defects resulting from inhibition of histone deacetylation. JF - Cell cycle (Georgetown, Tex.) AU - Robbins, April R AU - Jablonski, Sandra A AU - Yen, Tim J AU - Yoda, Kinya AU - Robey, Rob AU - Bates, Susan E AU - Sackett, Dan L AD - Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA. cwharr@helix.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 717 EP - 726 VL - 4 IS - 5 KW - Chromosomal Proteins, Non-Histone KW - 0 KW - Depsipeptides KW - Heterochromatin KW - Histone Deacetylase Inhibitors KW - Histones KW - Hydroxamic Acids KW - Indoles KW - Microcystins KW - Microfilament Proteins KW - Peptides, Cyclic KW - centromere protein E KW - centromere protein F KW - heterochromatin-specific nonhistone chromosomal protein HP-1 KW - 107283-02-3 KW - trichostatin A KW - 3X2S926L3Z KW - DAPI KW - 47165-04-8 KW - HC toxin KW - 83209-65-8 KW - romidepsin KW - CX3T89XQBK KW - Protein Kinases KW - EC 2.7.- KW - AURKB protein, human KW - EC 2.7.11.1 KW - Aurora Kinase B KW - Aurora Kinases KW - BUB1 protein, human KW - Bub1 spindle checkpoint protein KW - Protein-Serine-Threonine Kinases KW - Histone Deacetylases KW - EC 3.5.1.98 KW - cyanoginosin LR KW - EQ8332842Y KW - Methotrexate KW - YL5FZ2Y5U1 KW - Index Medicus KW - Protein Processing, Post-Translational -- drug effects KW - Protein Kinases -- analysis KW - Humans KW - Cell Division -- physiology KW - Chromosomal Proteins, Non-Histone -- metabolism KW - G2 Phase -- physiology KW - Chromosomal Proteins, Non-Histone -- analysis KW - Protein-Serine-Threonine Kinases -- analysis KW - Histone Deacetylases -- metabolism KW - Spindle Apparatus -- physiology KW - Peptides, Cyclic -- pharmacology KW - Hydroxamic Acids -- pharmacology KW - Protein-Serine-Threonine Kinases -- physiology KW - S Phase -- drug effects KW - Methotrexate -- pharmacology KW - Protein-Serine-Threonine Kinases -- drug effects KW - S Phase -- physiology KW - Cell Division -- drug effects KW - Cell Line, Tumor KW - Spindle Apparatus -- drug effects KW - Acetylation KW - G2 Phase -- drug effects KW - Depsipeptides -- pharmacology KW - Indoles -- pharmacology KW - Centromere -- physiology KW - Kinetochores -- drug effects KW - Heterochromatin -- drug effects KW - Kinetochores -- physiology KW - Heterochromatin -- physiology KW - Kinetochores -- chemistry KW - Chromosome Segregation -- drug effects KW - Heterochromatin -- chemistry KW - Histones -- metabolism KW - Mitosis -- drug effects KW - Centromere -- drug effects KW - Mitosis -- physiology KW - Histones -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67956052?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+cycle+%28Georgetown%2C+Tex.%29&rft.atitle=Inhibitors+of+histone+deacetylases+alter+kinetochore+assembly+by+disrupting+pericentromeric+heterochromatin.&rft.au=Robbins%2C+April+R%3BJablonski%2C+Sandra+A%3BYen%2C+Tim+J%3BYoda%2C+Kinya%3BRobey%2C+Rob%3BBates%2C+Susan+E%3BSackett%2C+Dan+L&rft.aulast=Robbins&rft.aufirst=April&rft.date=2005-05-01&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-03-03 N1 - Date created - 2005-06-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prenatal marijuana exposure: effect on child depressive symptoms at ten years of age. AN - 67911045; 15869861 AB - Studies of the consequences of prenatal marijuana use have reported effects predominantly on the behavioral and cognitive development of the children. Research on other aspects of child neurobehavioral development, such as psychiatric symptomatology, has been limited. This study examines the relations between prenatal marijuana exposure (PME) and child depressive symptoms at 10 years of age. Data are from the 10-year follow-up of 633 mother-child dyads who participated in the Maternal Health Practices and Child Development Project. Maternal prenatal and current substance use, measures of the home environment, demographic status, and psychosocial characteristics were ascertained at prenatal months four and seven, at delivery, and at age 10. At age 10, the children also completed the Children's Depression Inventory (CDI) [M. Kovacs. The Children's Depression Inventory, Multi-Health Systems, Inc., North Tonawanda, NY, (1992).], a self-report measure of current depressive symptoms. Multivariate regressions were used to test trimester-specific effects of marijuana and their associations with the CDI total score, while controlling for significant prenatal predictors and significant current covariates of childhood depression. PME in the first and third trimesters predicted significantly increased levels of depressive symptoms. This finding remained significant after controlling for all identified covariates from both the prenatal period and the current phase at age 10. These findings reflect an association with the level of depressive symptoms rather than a diagnosis of a major depressive disorder. Other significant correlates of depressive symptoms in the children included maternal education, maternal tobacco use (prenatal or current), and the child's composite IQ score. These findings are consistent with recent reports that identify specific areas of the brain and specific brain functions that are associated with PME. JF - Neurotoxicology and teratology AU - Gray, Kimberly A AU - Day, Nancy L AU - Leech, Sharon AU - Richardson, Gale A AD - Susceptibility and Population Health Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. PY - 2005 SP - 439 EP - 448 VL - 27 IS - 3 SN - 0892-0362, 0892-0362 KW - Index Medicus KW - Environment KW - Humans KW - Social Behavior KW - Mothers -- psychology KW - Child KW - Pregnancy KW - Socioeconomic Factors KW - Psychiatric Status Rating Scales KW - Pregnancy Trimester, First KW - Prospective Studies KW - Adult KW - Cohort Studies KW - Female KW - Male KW - Substance-Related Disorders -- epidemiology KW - Intelligence Tests KW - Depressive Disorder -- epidemiology KW - Depressive Disorder -- chemically induced KW - Cannabis -- adverse effects KW - Prenatal Exposure Delayed Effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67911045?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=proceeding&rft.jtitle=Neurotoxicology+and+teratology&rft.atitle=Prenatal+marijuana+exposure%3A+effect+on+child+depressive+symptoms+at+ten+years+of+age.&rft.au=Gray%2C+Kimberly+A%3BDay%2C+Nancy+L%3BLeech%2C+Sharon%3BRichardson%2C+Gale+A&rft.aulast=Gray&rft.aufirst=Kimberly&rft.date=2005-05-01&rft.volume=27&rft.issue=3&rft.spage=439&rft.isbn=&rft.btitle=&rft.title=Neurotoxicology+and+teratology&rft.issn=08920362&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-02 N1 - Date created - 2005-06-07 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Development of medications for alcohol use disorders: recent advances and ongoing challenges. AN - 67900958; 15934870 AB - During the past decade, efforts to develop medications for alcoholism have burgeoned. Three agents, disulfiram, naltrexone and acamprosate, are now approved in a large number of countries. Although many patients have benefited from existing medications, their effects are moderate, and some alcoholics fail to respond to them. A host of new agents are currently under active investigation. Critical barriers must be overcome to ensure that future efforts in the development of medications for alcohol use disorders reach full fruition. These challenges include: establishing key targets for drug discovery; validating animal and human screening models; and developing biomarkers to help predict treatment success. In addition, it is important to formulate methodological and statistical strategies to efficiently conduct alcohol pharmacotherapy trials; to specify genetic and phenotypic patient characteristics associated with efficacy and safety for lead compounds; to forge productive alliances among governmental agencies, the pharmaceutical industry and academic researchers to further drug development; and, ultimately and perhaps most difficult, to engage the practitioner community to incorporate medications into the alcohol treatment process. JF - Expert opinion on emerging drugs AU - Litten, Raye Z AU - Fertig, Joanne AU - Mattson, Margaret AU - Egli, Mark AD - Division of Treatment and Recovery Research, National Institute on Alcohol Abuse and Alcoholism, 5635 Fishers Lane, Room 2041, Bethesda, MD 20852-1705, USA. rlitten@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 323 EP - 343 VL - 10 IS - 2 KW - Drugs, Investigational KW - 0 KW - Index Medicus KW - Humans KW - Clinical Trials as Topic -- statistics & numerical data KW - Drugs, Investigational -- therapeutic use KW - Alcohol-Related Disorders -- physiopathology KW - Alcohol-Related Disorders -- drug therapy KW - Drug Industry -- trends KW - Drugs, Investigational -- chemistry KW - Alcohol-Related Disorders -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67900958?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Expert+opinion+on+emerging+drugs&rft.atitle=Development+of+medications+for+alcohol+use+disorders%3A+recent+advances+and+ongoing+challenges.&rft.au=Litten%2C+Raye+Z%3BFertig%2C+Joanne%3BMattson%2C+Margaret%3BEgli%2C+Mark&rft.aulast=Litten&rft.aufirst=Raye&rft.date=2005-05-01&rft.volume=10&rft.issue=2&rft.spage=323&rft.isbn=&rft.btitle=&rft.title=Expert+opinion+on+emerging+drugs&rft.issn=1744-7623&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-04-14 N1 - Date created - 2005-06-06 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Disease and injury among participants in the Agricultural Health Study. AN - 67891291; 15931940 AB - The Agricultural Health Study (www.aghealth.org) is a cohort of 89,658 pesticide applicators and their spouses from Iowa and North Carolina assembled between 1993 and 1997 to evaluate riskfactorsfor disease in ruralfarm populations. This prospective study is just now reaching sufficient maturity for analysis of many disease endpoints. Nonetheless, several analyses have already provided interesting and important leads regarding disease patterns in agricultural populations and etiologic clues for the general population. Compared to the mortality experience of the general population in the two states (adjusted for race, gender, age and calendar time), the cohort experienced a very low mortality rate overall and for many specific causes and a low rate of overall cancer incidence. A few cancers, however, appear elevated, including multiple myeloma and cancers of the lip, gallbladder, ovary, prostate, and thyroid, but numbers are small for many cancers. A study of prostate cancer found associations with exposure to several pesticides, particularly among individuals with a family history of prostate cancer. Links to pesticides and other agricultural factors have been found for injuries, retinal degeneration, and respiratory wheeze. Methodological studies have determined that information collected by interview is unbiased and reliable. A third round of interviews scheduled to begin in 2005 will collect additional information on agricultural exposures and health outcomes. The study can provide data to address many health issues in the agricultural community. The study investigators welcome collaboration with interested scientists. JF - Journal of agricultural safety and health AU - Blair, A AU - Sandler, D AU - Thomas, K AU - Hoppin, J A AU - Kamel, F AU - Coble, J AU - Lee, W J AU - Rusiecki, J AU - Knott, C AU - Dosemeci, M AU - Lynch, C F AU - Lubin, J AU - Alavanja, M AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Executive Plaza South, Room 8118, Bethesda, Maryland 20892 , USA. blaira@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 141 EP - 150 VL - 11 IS - 2 SN - 1074-7583, 1074-7583 KW - Pesticides KW - 0 KW - Index Medicus KW - Prospective Studies KW - Risk Factors KW - Humans KW - Accidents, Occupational -- statistics & numerical data KW - Wounds and Injuries KW - Interviews as Topic KW - Middle Aged KW - North Carolina -- epidemiology KW - Male KW - Iowa -- epidemiology KW - Female KW - Spouses KW - Agricultural Workers' Diseases -- mortality KW - Agricultural Workers' Diseases -- etiology KW - Agricultural Workers' Diseases -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67891291?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+agricultural+safety+and+health&rft.atitle=Disease+and+injury+among+participants+in+the+Agricultural+Health+Study.&rft.au=Blair%2C+A%3BSandler%2C+D%3BThomas%2C+K%3BHoppin%2C+J+A%3BKamel%2C+F%3BCoble%2C+J%3BLee%2C+W+J%3BRusiecki%2C+J%3BKnott%2C+C%3BDosemeci%2C+M%3BLynch%2C+C+F%3BLubin%2C+J%3BAlavanja%2C+M&rft.aulast=Blair&rft.aufirst=A&rft.date=2005-05-01&rft.volume=11&rft.issue=2&rft.spage=141&rft.isbn=&rft.btitle=&rft.title=Journal+of+agricultural+safety+and+health&rft.issn=10747583&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-06-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Am J Epidemiol. 2003 May 1;157(9):800-14 [12727674] Occup Environ Med. 2003 Aug;60(8):e3 [12883030] Scand J Work Environ Health. 2005;31 Suppl 1:39-45; discussion 5-7 [16190148] Environ Health Perspect. 2004 Apr;112(5):631-5 [15064173] J Natl Cancer Inst. 2004 Sep 15;96(18):1375-82 [15367570] Am J Epidemiol. 2004 Feb 15;159(4):373-80 [14769641] Occup Med. 1991 Jul-Sep;6(3):327-34 [1948522] Scand J Work Environ Health. 1992 Aug;18(4):209-15 [1411362] Am J Ind Med. 1997 Feb;31(2):233-42 [9028440] Environ Health Perspect. 1998 Jul;106(7):415-20 [9637799] Environ Res. 1999 Feb;80(2 Pt 1):172-9 [10092410] Ann Epidemiol. 2005 Apr;15(4):279-85 [15780775] J Expo Anal Environ Epidemiol. 2005 May;15(3):225-33 [15280893] Am J Respir Crit Care Med. 2002 Mar 1;165(5):683-9 [11874814] Epidemiology. 2002 Jan;13(1):94-9 [11805592] Am J Ind Med. 2000 Jun;37(6):618-28 [10797505] J Agric Saf Health. 2003 Feb;9(1):5-18 [12673912] J Expo Anal Environ Epidemiol. 2002 Nov;12(6):418-26 [12415490] Ann Occup Hyg. 2002 Mar;46(2):245-60 [12074034] Am J Epidemiol. 2004 Nov 1;160(9):876-85 [15496540] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Two C-methyl derivatives of [11C]WAY-100635--effects of an amido alpha-methyl group on metabolism and brain 5-HT1A receptor radioligand behavior in monkey. AN - 67865927; 15912425 AB - [carbonyl-11C]N-(2-(1-(4-(2-methoxyphenyl)-piperazinyl)ethyl)-N-pyridinyl)cyclohexanecarboxamide ([carbonyl-11C]WAY-100635) is an effective radioligand for imaging brain 5-HT1A receptors with positron emission tomography (PET). However, this radioligand has some drawbacks for deriving relative regional receptor densities, including rapid metabolism, which acts against accurate definition of an arterial input function for compartmental modeling, and very low nonspecific binding in brain, which detracts from the accuracy of modeling by a simplified reference tissue (cerebellum) approach. Here, in a search for a radioligand that overcomes these limitations, we investigated the effects of introducing a single methyl group at either of the carbon atoms alpha to the amide bond in [11C]WAY-100635. Ligands with a methyl group on the alpha carbon of the cyclohexyl group (SWAY) or the alpha carbon of the C2H4 linker ((R,S)-JWAY) were synthesized and tested for binding affinity and intrinsic activity at 5-HT1A receptors. SWAY was labeled with carbon-11 (t1/2 = 20.4 minutes; beta+ = 99.8%) in its O-methyl group and (R,S)-JWAY in its carbonyl group. Each radioligand was evaluated by PET experiments in cynomolgus monkey. SWAY and (R,S)-JWAY were found to be high-affinity antagonists at 5-HT1A receptors. After injection of [11C]SWAY into monkey, radioactivity uptake in brain reached a maximum of 3% at 4.5 minutes and decreased to 0.7% at 72 minutes. However, over the time span of the experiment, radioactivity concentrations in 5-HT1A receptor-rich brain regions were only fractionally higher than in cerebellum. Radioactivity represented by parent radioligand in plasma was 39% at 45 minutes. After injection of [11C](R,S)-JWAY alone, radioactivity uptake in brain reached a maximum of 4.8% at 2.5 minutes and decreased to 1.2% at 90 minutes. At this time, radioactivity concentration in 5-HT1A receptor-rich brain regions was markedly greater than in cerebellum. In another PET experiment, the monkey was predosed with WAY-100635 before [11C](R,S)-JWAY injection. At 90 minutes after injection, the ratio of radioactivity in 5-HT1A receptor-rich regions to that in cerebellum was reduced to near unity. Radioactivity represented by parent radioligand in plasma was 12% at 45 minutes. [11C](R,S)-JWAY, but not [11C]SWAY, gives a sizeable 5-HT1A receptor-selective PET signal in monkey. The presence of a C-methyl group adjacent to the amide bond in SWAY or (R,S)-JWAY fails to counter metabolism. JF - Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging AU - McCarron, Julie A AU - Marchais-Oberwinkler, Sandrine AU - Pike, Victor W AU - Tarkiainen, Jari AU - Halldin, Christer AU - Sóvagó, Judit AU - Gulyas, Balàzs AU - Wikström, Hakan V AU - Farde, Lars AD - MRC Cyclotron Unit, Imperial College School of Medicine, Hammersmith Hospital, Ducane Road, London, W12 0NN, UK. mccarronj@intra.nimh.nih.gov PY - 2005 SP - 209 EP - 219 VL - 7 IS - 3 SN - 1536-1632, 1536-1632 KW - Carbon Radioisotopes KW - 0 KW - Ligands KW - Lipids KW - Piperazines KW - Pyridines KW - Receptors, Serotonin, 5-HT1 KW - Serotonin 5-HT1 Receptor Antagonists KW - N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide KW - 71IH826FEG KW - Index Medicus KW - Radiochemistry KW - Molecular Structure KW - Hydrophobic and Hydrophilic Interactions KW - Animals KW - Positron-Emission Tomography KW - Lipids -- chemistry KW - Inhibitory Concentration 50 KW - Methylation KW - Carbon Radioisotopes -- chemistry KW - Amination KW - Receptors, Serotonin, 5-HT1 -- metabolism KW - Pyridines -- chemistry KW - Piperazines -- chemistry KW - Brain -- drug effects KW - Pyridines -- pharmacokinetics KW - Brain -- metabolism KW - Radioligand Assay KW - Haplorhini -- metabolism KW - Piperazines -- pharmacokinetics KW - Piperazines -- chemical synthesis KW - Pyridines -- chemical synthesis KW - Pyridines -- blood KW - Piperazines -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67865927?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Validation+status+of+the+hens+egg+test-chorioallantoic+membrane+%28HET-CAM%29+test+method&rft.au=Choksi%2C+N%3BAllen%2C+D%3BInhof%2C+C%3BTruax%2C+J%3BTice%2C+R%3BStokes%2C+W&rft.aulast=Choksi&rft.aufirst=N&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Methadone for cancer pain: what have we learned from clinical studies? AN - 67860460; 15909785 AB - The analgesic ladder guideline proposed by the World Health Organization has been shown to be effective in controlling cancer pain in about 80 percent of patients, but the remaining 20 percent still experience pain. Several strategies have been used to manage refractory cancer pain and opioid toxicity. Switching opioids, alternative routes of opioid administration, optimizing adjuvants, and invasive procedures are proposed treatments. Extensive medical literature corroborates each one of those treatments. Rotation from one opioid to another is a noninvasive strategy to overcome opioid side effects and refractory pain. Frequently, methadone is used during opioid rotation. However, there is a lack of consensus on how to proceed on rotation from morphine to methadone. In the current era of evidence-based medicine, the medical literature fails to answer some cancer pain-management issues. The purpose of this review is to clarify a process for transitioning from morphine to methadone. JF - The American journal of hospice & palliative care AU - Soares, Luiz Guilherme L AD - Pain Clinic, National Cancer Institute, Rio de Janeiro, Brazil. PY - 2005 SP - 223 EP - 227 VL - 22 IS - 3 SN - 1049-9091, 1049-9091 KW - Analgesics, Opioid KW - 0 KW - Morphine KW - 76I7G6D29C KW - Methadone KW - UC6VBE7V1Z KW - Nursing KW - World Health Organization KW - Morphine -- therapeutic use KW - Dose-Response Relationship, Drug KW - Humans KW - Pain Measurement KW - Pain -- etiology KW - Methadone -- adverse effects KW - Pain -- drug therapy KW - Neoplasms -- complications KW - Methadone -- therapeutic use KW - Analgesics, Opioid -- therapeutic use KW - Methadone -- administration & dosage KW - Analgesics, Opioid -- adverse effects KW - Analgesics, Opioid -- administration & dosage KW - Palliative Care -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67860460?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+hospice+%26+palliative+care&rft.atitle=Methadone+for+cancer+pain%3A+what+have+we+learned+from+clinical+studies%3F&rft.au=Soares%2C+Luiz+Guilherme+L&rft.aulast=Soares&rft.aufirst=Luiz+Guilherme&rft.date=2005-05-01&rft.volume=22&rft.issue=3&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+hospice+%26+palliative+care&rft.issn=10499091&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-05-24 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Am J Hosp Palliat Care. 2005 Sep-Oct;22(5):337 [16225353] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - VEGF contributes to mammary tumor growth in transgenic mice through paracrine and autocrine mechanisms. AN - 67850508; 15765121 AB - Vascular endothelial growth factor (VEGF) has been identified as a vascular permeability factor, angiogenic cytokine, and a survival factor. To address its role in mammary carcinogenesis, we used transgenic mice with human VEGF(165) targeted to mammary epithelial cells under the control of the mouse mammary tumor virus (MMTV) promoter. Metastatic mammary carcinomas were induced by mating the MMTV-VEGF mice with MMTV-polyoma virus middle T-antigen (MT) mice to generate VEGF/MT mice. Tumor latency was decreased in the VEGF/MT mice, which developed mammary carcinomas with increased vasodilatation at 4 weeks of age. There was increased incidence, multiplicity, and weight of the mammary tumors in 6- and 8-week-old VEGF/MT mice, compared to their MT-only littermates. Macro- and microscopic lung metastases were detected in the VEGF/MT mice but not the MT mice at 6 and 8 weeks of age. Enhanced tumor growth was attributed to increased microvascular density (MVD), as well as increased tumor cell proliferation and survival. Angiogenesis array analysis showed that 24 of 25 differentially expressed genes were upregulated in the VEGF/MT tumors. In vitro studies revealed increased proliferative activity and upregulation of Flk-1 in the VEGF/MT tumor cells, compared with the MT-only tumor cells. Moreover, there was decreased proliferative activity with downregulation of Flk-1 in tumor cells isolated from conditional knockout (VEGF(-/-)) MT-induced mammary carcinomas. The slow growing VEGF(-/-) tumor cells were accumulated in the G(1)/G(0) phase of the cell cycle and this was associated with stimulation of p16(ink4a) and p21(WAF1). Similarly, p16(ink4a) was stimulated in VEGF(lox/lox)/MT mammary tumor cells following Adeno-cre-mediated VEGF gene inactivation. Collectively, the data from these transgenic models indicate that VEGF contributes to mammary tumor growth through increased neovascularization, as well as autocrine stimulation of growth and inhibition of apoptosis. JF - Laboratory investigation; a journal of technical methods and pathology AU - Schoeffner, Daniel J AU - Matheny, Shannon L AU - Akahane, Takemi AU - Factor, Valentina AU - Berry, Adam AU - Merlino, Glenn AU - Thorgeirsson, Unnur P AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 608 EP - 623 VL - 85 IS - 5 SN - 0023-6837, 0023-6837 KW - Cdkn1a protein, mouse KW - 0 KW - Cell Cycle Proteins KW - Cyclin-Dependent Kinase Inhibitor p21 KW - RNA, Messenger KW - RNA, Neoplasm KW - Vascular Endothelial Growth Factor A KW - vascular endothelial growth factor A, mouse KW - Vascular Endothelial Growth Factor Receptor-2 KW - EC 2.7.10.1 KW - Index Medicus KW - Animals KW - Blotting, Northern KW - Oligonucleotide Array Sequence Analysis KW - Lung Neoplasms -- secondary KW - Vascular Endothelial Growth Factor Receptor-2 -- genetics KW - Mice KW - Reverse Transcriptase Polymerase Chain Reaction KW - RNA, Neoplasm -- analysis KW - Mice, Transgenic KW - Cell Proliferation KW - Genes, p16 KW - Mice, Knockout KW - Apoptosis -- genetics KW - RNA, Messenger -- metabolism KW - Cell Cycle Proteins -- genetics KW - Lung Neoplasms -- genetics KW - Up-Regulation KW - Vascular Endothelial Growth Factor Receptor-2 -- metabolism KW - Immunohistochemistry KW - Male KW - Female KW - Lung Neoplasms -- metabolism KW - Adenocarcinoma -- metabolism KW - Mammary Neoplasms, Animal -- pathology KW - Mammary Neoplasms, Animal -- genetics KW - Adenocarcinoma -- secondary KW - Mammary Neoplasms, Animal -- metabolism KW - Adenocarcinoma -- genetics KW - Vascular Endothelial Growth Factor A -- genetics KW - Vascular Endothelial Growth Factor A -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67850508?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Aberrant+gene+expression+in+the+neonatal+mouse+lung+following+in+utero+exposure+to+inorganic+arsenic&rft.au=Shen%2C+J%3BXie%2C+Y%3BLiu%2C+J%3BDiwan%2C+B%3BWaalkes%2C+M&rft.aulast=Shen&rft.aufirst=J&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-05-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Prevalence and motives for illicit use of prescription stimulants in an undergraduate student sample. AN - 67844360; 15900989 AB - To assess the prevalence and motives for illicit use of prescription stimulants and alcohol and other drugs (AODs), associated with these motives, the authors distributed a self-administered Web survey TO a random sample of 9,161 undergraduate college students. Of the study participants, 8.1% reported lifetime and 5.4% reported past-year illicit use of prescription stimulants. The most prevalent motives given for illicit use of prescription stimulants were to (1) help with concentration, (2) increase alertness, and (3) provide a high. Although men were more likely than women were to report illicit use of prescription stimulants, the authors found no gender differences in motives. Regardless of motive, illicit use of prescription stimulants was associated with elevated rates of AOD use, and number of motives endorsed and AOD use were positively related. Students appear to be using these prescription drugs non-medically, mainly to enhance performance or get high. JF - Journal of American college health : J of ACH AU - Teter, Christian J AU - McCabe, Sean Esteban AU - Cranford, James A AU - Boyd, Carol J AU - Guthrie, Sally K AD - National Institute on Drug Abuse, College of Pharmacy and Substance Abuse Research Center at the University of Michigan, Ann Arbor, USA. c.teter@neu.edu PY - 2005 SP - 253 EP - 262 VL - 53 IS - 6 SN - 0744-8481, 0744-8481 KW - Central Nervous System Stimulants KW - 0 KW - Street Drugs KW - Index Medicus KW - Motivation KW - Attitude to Health KW - Risk Factors KW - Humans KW - Chi-Square Distribution KW - Surveys and Questionnaires KW - United States -- epidemiology KW - Male KW - Internet KW - Female KW - Prevalence KW - Students -- psychology KW - Self Disclosure KW - Students -- statistics & numerical data KW - Central Nervous System Stimulants -- administration & dosage KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67844360?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Gingival+carcinogenicity+in+female+Harlan+Sprague-Dawley+rats+after+oral+treatment+for+two+years+with+2%2C+3%2C+7%2C+8-tetrachlorodibenzo-p-dioxin+and+dioxin-like+compounds&rft.au=Yoshizawa%2C+K%3BWalker%2C+N+J%3BJokinen%2C+M+P%3BBrix%2C+A+E%3BSells%2C+D+M%3BMarsh%2C+T%3BWyde%2C+ME%3BOrzech%2C+D%3BHaseman%2C+J+K%3BNyska%2C+A&rft.aulast=Yoshizawa&rft.aufirst=K&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tumor vaccine: current trends in antigen specific immunotherapy. AN - 67840988; 15900903 AB - Effective cancer treatment to prevent the tumor growth as well as to stop its recurrence is the dream of oncologists. Currently available therapeutic measures like, radiotherapy and chemotherapy, often suffer from severe toxicity and lack of specificity of the drug towards tumor cells. Another promising approach is the 'immunotherapy', in which either the immune system is activated by tumor vaccine to combat the tumor growth or antitumor antibodies can be used. Vaccination can stimulate humoral, cellular and innate immune systems to generate various effector molecules, like antibody, cytotoxic T cells, cytokines etc. In antigen specific immunotherapy, the immune system can be stimulated actively by antigen based tumor vaccine to kill only those tumor cells, having expression of the particular tumor associated antigen. Different experimental, preclinical and clinical studies have proved that generated immune responses are effective to restrict the tumor growth. Useful strategies of antigen specific immunotherapy and outcome of various laboratory and clinic based studies are discussed. JF - Indian journal of experimental biology AU - Baral, Rathindranath AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata 700 026, India. rbaral@hotmail.com Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 389 EP - 406 VL - 43 IS - 5 SN - 0019-5189, 0019-5189 KW - Antigens, Neoplasm KW - 0 KW - Cancer Vaccines KW - Index Medicus KW - Humans KW - T-Lymphocytes -- immunology KW - Cancer Vaccines -- immunology KW - Immunotherapy KW - Cancer Vaccines -- therapeutic use KW - Neoplasms -- therapy KW - Antigens, Neoplasm -- immunology KW - Neoplasms -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67840988?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Indian+journal+of+experimental+biology&rft.atitle=Tumor+vaccine%3A+current+trends+in+antigen+specific+immunotherapy.&rft.au=Baral%2C+Rathindranath&rft.aulast=Baral&rft.aufirst=Rathindranath&rft.date=2005-05-01&rft.volume=43&rft.issue=5&rft.spage=389&rft.isbn=&rft.btitle=&rft.title=Indian+journal+of+experimental+biology&rft.issn=00195189&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-05-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Quantifying the risks associated with exceeding recommended drinking limits. AN - 67834313; 15897737 AB - Although daily and weekly drinking limits demonstrate strong sensitivity and specificity in identifying alcohol use disorders (AUDs), there are no descriptive data that present the risks associated with exceeding these limits in a format suitable for presentation to patients, students, and the general public. Data collected in the 2001-2002 National Epidemiologic Survey on Alcohol and Related Conditions were used to estimate the risks of past-year DSM-IV alcohol abuse and dependence associated with various frequencies of exceeding daily drinking limits (no more than 4 drinks for men; no more than 3 drinks for women) in a nationally representative sample of 26,946 US drinkers 18 years of age and older. These risks were further categorized by whether weekly drinking limits (no more than 14 drinks for men; no more than 7 drinks for women) were exceeded and by maximum number of drinks consumed in the past year. The prevalence of alcohol dependence with abuse increased in a fairly linear fashion with frequency of exceeding daily drinking limits. The prevalence of dependence alone (no abuse) and abuse alone (no dependence) peaked among persons who exceeded the daily limits twice a week and then leveled off, because individuals became increasingly likely to have both disorders at higher frequencies. Exceeding the weekly limits generally increased the risks of both disorders after accounting for frequency of exceeding the daily limits, but not always to a significant extent. Likewise, maximum quantity of drinks consumed was positively associated with the risks of AUDs even after accounting for frequency of risk drinking. There were few gender differences in the risk of dependence after adjusting for frequency of exceeding daily drinking limits, but the risk of alcohol abuse remained greater among men. These data provide a useful tool for illustrating the broad range of risk of AUDs associated with exceeding recommended drinking limits, and they support the utility of the daily and weekly drinking limits in predicting AUDs. JF - Alcoholism, clinical and experimental research AU - Dawson, Deborah A AU - Grant, Bridget F AU - Li, Ting-Kai AD - Laboratory of Epidemiology and Biometry Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892-9304, USA. ddawson@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 902 EP - 908 VL - 29 IS - 5 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Psychiatric Status Rating Scales KW - Sex Factors KW - Humans KW - Adult KW - United States -- epidemiology KW - Male KW - Female KW - Risk Assessment KW - Alcoholism -- epidemiology KW - Alcohol Drinking -- psychology KW - Alcohol Drinking -- epidemiology KW - Alcoholism -- psychology KW - Alcoholism -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67834313?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Quantifying+the+risks+associated+with+exceeding+recommended+drinking+limits.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BLi%2C+Ting-Kai&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2005-05-01&rft.volume=29&rft.issue=5&rft.spage=902&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The anti-invasive activity of cyclooxygenase inhibitors is regulated by the transcription factor ATF3 (activating transcription factor 3). AN - 67833343; 15897233 AB - We previously showed that nonsteroidal anti-inflammatory drugs (NSAID) such as sulindac sulfide, which has chemopreventive activity, modulate the expression of several genes detected by microarray analysis. Activating transcription factor 3 (ATF3) was selected for further study because it is a transcription factor involved in cell proliferation, apoptosis, and invasion, and its expression is repressed in human colorectal tumors as compared with normal adjacent tissue. In this report, we show that ATF3 mRNA and protein expression are up-regulated in HCT-116 human colorectal cancer cells following treatment with NSAIDs, troglitazone, diallyl disulfide, and resveratrol. To ascertain the biological significance of ATF3, we overexpressed full-length ATF3 protein in the sense and antisense orientations. Overexpression of ATF3 in the sense orientation decreased focus formation in vitro and reduced the size of mouse tumor xenografts by 54% in vivo. Conversely, overexpression of antisense ATF3 was protumorigenic in vitro, however, not in vivo. ATF3 in the sense orientation did not modulate apoptosis, indicating another mechanism is involved. With microarray analysis, several genes relating to invasion and metastasis were identified by ATF3 overexpression and were confirmed by real-time reverse transcription-PCR, and several of these genes were modulated by sulindac sulfide, which inhibited invasion in these cells. Furthermore, overexpression of ATF3 inhibited invasion to a similar degree as sulindac sulfide treatment, whereas antisense ATF3 increased invasion. In conclusion, ATF3 represents a novel mechanism in which NSAIDs exert their anti-invasive activity, thereby linking ATF3 and its gene regulatory activity to the biological activity of these compounds. JF - Molecular cancer therapeutics AU - Bottone, Frank G AU - Moon, Yuseok AU - Kim, Jong Sik AU - Alston-Mills, Brenda AU - Ishibashi, Minako AU - Eling, Thomas E AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, P.O. Box 12233, 111 T.W. Alexander Drive, Research Triangle Park, NC 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 693 EP - 703 VL - 4 IS - 5 SN - 1535-7163, 1535-7163 KW - Activating Transcription Factor 3 KW - 0 KW - Allyl Compounds KW - Anti-Inflammatory Agents, Non-Steroidal KW - Chromans KW - Cyclooxygenase Inhibitors KW - Disulfides KW - RNA, Messenger KW - Stilbenes KW - Thiazolidinediones KW - Transcription Factors KW - Sulindac KW - 184SNS8VUH KW - diallyl disulfide KW - 5HI47O6OA7 KW - sulindac sulfide KW - 6UVA8S2DEY KW - troglitazone KW - I66ZZ0ZN0E KW - resveratrol KW - Q369O8926L KW - Index Medicus KW - Animals KW - Stilbenes -- pharmacology KW - Disulfides -- pharmacology KW - Humans KW - Allyl Compounds -- pharmacology KW - Mice, Nude KW - Mice KW - HCT116 Cells KW - RNA, Messenger -- genetics KW - Gene Expression Regulation, Neoplastic -- drug effects KW - RNA, Messenger -- metabolism KW - Chromans -- pharmacology KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Apoptosis -- drug effects KW - Thiazolidinediones -- pharmacology KW - Microarray Analysis KW - Transplantation, Heterologous KW - Up-Regulation KW - Male KW - Transcription Factors -- metabolism KW - Neoplasm Invasiveness -- pathology KW - Colorectal Neoplasms -- pathology KW - Colorectal Neoplasms -- metabolism KW - Sulindac -- pharmacology KW - Sulindac -- analogs & derivatives KW - Transcription Factors -- genetics KW - Cyclooxygenase Inhibitors -- pharmacology KW - Anti-Inflammatory Agents, Non-Steroidal -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67833343?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+therapeutics&rft.atitle=The+anti-invasive+activity+of+cyclooxygenase+inhibitors+is+regulated+by+the+transcription+factor+ATF3+%28activating+transcription+factor+3%29.&rft.au=Bottone%2C+Frank+G%3BMoon%2C+Yuseok%3BKim%2C+Jong+Sik%3BAlston-Mills%2C+Brenda%3BIshibashi%2C+Minako%3BEling%2C+Thomas+E&rft.aulast=Bottone&rft.aufirst=Frank&rft.date=2005-05-01&rft.volume=4&rft.issue=5&rft.spage=693&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-09 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Aspirin use and risk of biliary tract cancer: a population-based study in Shanghai, China. AN - 67833038; 15894693 AB - The association of gallbladder and bile duct cancers with gallstones, cholecystitis, and cholangitis suggest that chronic inflammation contributes to the carcinogenic process. However, the effect of nonsteroidal anti-inflammatory drugs, such as aspirin, on biliary tract cancer has not been well studied. In a population-based case-control study conducted in Shanghai, China, we examined the relationship between aspirin use and the risk of biliary disease. A total of 627 patients with biliary tract cancer, including cancers of the gallbladder (n = 368), extrahepatic bile duct (n = 191), and ampulla of Vater (n = 68); 1,037 patients with biliary stones; and 958 healthy adults were included in the study. Self-reported data on aspirin use was collected from study participants by in-person interview. The prevalence of aspirin use was low, with 5.7% of the population controls being regular users. After controlling for age, sex, education, and biliary stone status, aspirin use was associated with a reduced risk of gallbladder cancer [odds ratio (OR), 0.37; 95% confidence interval (CI), 0.17-0.88]. An inverse relationship was also observed for frequency and duration of use and with younger age when starting use. In addition, there was a nonsignificant reduction in the risk of bile duct (OR, 0.48; 95% CI, 0.19-1.19) and ampullary cancers (OR, 0.22; 95% CI, 0.03-1.65) associated with aspirin use, whereas no clear association was seen with biliary stones (OR, 0.92; 95% CI, 0.59-1.44). Further studies of biliary tract cancer in other populations are needed to confirm these results and to elucidate the mechanisms that underlie the reduced risk associated with use of aspirin and possibly other nonsteroidal anti-inflammatory drugs. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Liu, Enju AU - Sakoda, Lori C AU - Gao, Yu-Tang AU - Rashid, Asif AU - Shen, Ming-Chang AU - Wang, Bing-Sheng AU - Deng, Jie AU - Han, Tian-Quan AU - Zhang, Bai-He AU - Fraumeni, Joseph F AU - Hsing, Ann W AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Department of Health and Human Services, 6120 Executive Boulevard, EPS 7058, Bethesda, MD 20892-7234, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1315 EP - 1318 VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - Anti-Inflammatory Agents, Non-Steroidal KW - 0 KW - Aspirin KW - R16CO5Y76E KW - Index Medicus KW - Sex Factors KW - Humans KW - Aged KW - Gallstones -- diagnosis KW - Gallstones -- complications KW - Risk Factors KW - China -- epidemiology KW - Adult KW - Case-Control Studies KW - Interviews as Topic KW - Middle Aged KW - Chemoprevention KW - Gallstones -- surgery KW - Female KW - Male KW - Biliary Tract Neoplasms -- epidemiology KW - Anti-Inflammatory Agents, Non-Steroidal -- therapeutic use KW - Aspirin -- administration & dosage KW - Aspirin -- therapeutic use KW - Biliary Tract Neoplasms -- prevention & control KW - Biliary Tract Neoplasms -- complications KW - Anti-Inflammatory Agents, Non-Steroidal -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67833038?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Aspirin+use+and+risk+of+biliary+tract+cancer%3A+a+population-based+study+in+Shanghai%2C+China.&rft.au=Liu%2C+Enju%3BSakoda%2C+Lori+C%3BGao%2C+Yu-Tang%3BRashid%2C+Asif%3BShen%2C+Ming-Chang%3BWang%2C+Bing-Sheng%3BDeng%2C+Jie%3BHan%2C+Tian-Quan%3BZhang%2C+Bai-He%3BFraumeni%2C+Joseph+F%3BHsing%2C+Ann+W&rft.aulast=Liu&rft.aufirst=Enju&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1315&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Smoking is a risk factor for cervical intraepithelial neoplasia grade 3 among oncogenic human papillomavirus DNA-positive women with equivocal or mildly abnormal cytology. AN - 67832896; 15894667 AB - Smoking is a potential risk factor for cervical cancer and its immediate precursor, cervical intraepithelial neoplasia grade 3 (CIN3), but few studies have adequately taken into account the possible confounding effect of oncogenic human papillomavirus (HPV) infection. Women (n = 5,060) with minimally abnormal Papanicolaou smears were enrolled in the ASCUS and LSIL Triage Study, a clinical trial to evaluate management strategies, and were seen every 6 months for the 2-year duration of the study. Cervical specimens were tested for HPV DNA using both Hybrid Capture 2 and PGMY09/11 L1 consensus primer PCR with reverse line blot hybridization for genotyping. Multivariate logistics regression models were used to assess associations [odds ratio (OR) with 95% confidence intervals (95% CI)] between smoking behaviors and rigorously reviewed cases of cervical intraepithelial neoplasia grade 3 or cancer (> or =CIN3) identified throughout the study (n = 506) in women with oncogenic HPV (n = 3,133). Current smoking was only weakly associated with increased HPV infection. Among infected women, current smokers (OR, 1.7; 95% CI, 1.4-2.1) and past smokers (OR, 1.7; 95% CI, 1.2-2.4) were more likely to be diagnosed with > or =CIN3 than nonsmokers. Greater smoking intensity (P(Trend) or =2 packs/d (OR, 3.3; 95% CI, 1.5-7.5) and smoking for > or =11 years (OR, 2.1; 95% CI, 1.5-2.9) most strongly associated with > or =CIN3 as compared to non-smokers. The effects of intensity and duration seemed additive. Women with oncogenic HPV and minimally abnormal Papanicolaou smears who smoke were up to three times more likely to be diagnosed with > or =CIN3 than nonsmokers. Smoking cessation trials targeting this population might be warranted. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - McIntyre-Seltman, Kathleen AU - Castle, Philip E AU - Guido, Richard AU - Schiffman, Mark AU - Wheeler, Cosette M AU - ALTS Group AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Room 7074, 6120 Executive Boulevard, EPS MSC 7234, Bethesda, MD 20892-7234, USA. ; ALTS Group Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1165 EP - 1170 VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - DNA, Viral KW - 0 KW - Nicotine KW - 6M3C89ZY6R KW - Index Medicus KW - Neoplasm Staging KW - Nicotine -- toxicity KW - Humans KW - Aged KW - Vaginal Smears -- methods KW - Genotype KW - Polymerase Chain Reaction KW - Prospective Studies KW - Oncogenes KW - Triage KW - Aged, 80 and over KW - Logistic Models KW - Risk Factors KW - Adult KW - Middle Aged KW - Adolescent KW - DNA, Viral -- genetics KW - Female KW - Papanicolaou Test KW - Papillomavirus Infections -- epidemiology KW - Papillomaviridae -- classification KW - Uterine Cervical Neoplasms -- epidemiology KW - Papillomavirus Infections -- complications KW - Smoking -- adverse effects KW - Cervical Intraepithelial Neoplasia -- virology KW - Papillomaviridae -- genetics KW - Smoking -- epidemiology KW - Uterine Cervical Neoplasms -- virology KW - Papillomaviridae -- drug effects KW - Cervical Intraepithelial Neoplasia -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67832896?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Smoking+is+a+risk+factor+for+cervical+intraepithelial+neoplasia+grade+3+among+oncogenic+human+papillomavirus+DNA-positive+women+with+equivocal+or+mildly+abnormal+cytology.&rft.au=McIntyre-Seltman%2C+Kathleen%3BCastle%2C+Philip+E%3BGuido%2C+Richard%3BSchiffman%2C+Mark%3BWheeler%2C+Cosette+M%3BALTS+Group&rft.aulast=McIntyre-Seltman&rft.aufirst=Kathleen&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1165&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-12-12 N1 - Date created - 2005-05-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effectiveness of the derived Alcohol Use Disorders Identification Test (AUDIT-C) in screening for alcohol use disorders and risk drinking in the US general population. AN - 67829981; 15897730 AB - The three consumption questions from the Alcohol Use Disorders Identification Test (AUDIT-C) are increasingly used as a screener for alcohol use disorders (AUDs) and risk drinking. In a representative sample of US adults 18 years of age and older, AUDIT-C scores (derived from consumption questions embedded in a large national survey) were used to estimate sensitivity, specificity, and areas under receiver operator characteristic curves (AUROCs) for alcohol dependence, any AUD, and risk drinking. AUDs were defined according to DSM-IV criteria. For men, risk drinking was defined as consuming >14 drinks per week or >4 drinks in a single day at least once a month; for women, the weekly and daily limits were >7 drinks and >3 drinks, respectively. The derived AUDIT-C was evaluated among past-year drinkers (n = 26,946), within the total population (n = 43,093), in groups defined by age, sex, and race/ethnicity, and among pregnant women, persons attending an emergency room, and college students. For past-year drinkers, the AUROCs for the derived AUDIT-C were 0.887 for alcohol dependence, 0.860 for any AUD, and 0.966 for risk drinking. Scores were higher in the total population, 0.931, 0.917, and 0.981, respectively. The derived AUDIT-C performed slightly better in screening for dependence among women than men. Screening for risk drinking was better among men, probably because the third AUDIT-C question directly mirrors one of the definitions of risk drinking for men but not for women. Performance in pregnant women, past-year emergency room patients, and college students was on a par with performance in the general population. The derived AUDIT-C performs well in screening for AUDs and risk drinking. The use of variable cut points for men and women improves its sensitivity and specificity. Validation in a realistic screening situation, in which the AUDIT-C questions are asked as stand-alone and not embedded items, is a critical future step. JF - Alcoholism, clinical and experimental research AU - Dawson, Deborah A AU - Grant, Bridget F AU - Stinson, Frederick S AU - Zhou, Yuan AD - Laboratory of Epidemiology and Biometry, Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892-9304, USA. ddawson@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 844 EP - 854 VL - 29 IS - 5 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Age Factors KW - Risk-Taking KW - ROC Curve KW - Humans KW - Aged KW - Pregnancy KW - Psychiatric Status Rating Scales KW - Ethnic Groups KW - Adult KW - Middle Aged KW - Adolescent KW - United States -- epidemiology KW - Female KW - Male KW - Alcoholism -- epidemiology KW - Alcoholism -- diagnosis KW - Alcohol Drinking -- psychology KW - Alcohol Drinking -- epidemiology KW - Alcoholism -- psychology KW - Psychological Tests UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67829981?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Histone+modifications+in+enhancer+activation+and+enhancer+blocking&rft.au=Dean%2C+A&rft.aulast=Dean&rft.aufirst=A&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-27 N1 - Date created - 2005-05-17 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Factor analysis of pesticide use patterns among pesticide applicators in the Agricultural Health Study. AN - 67821697; 15280893 AB - Exposure to certain pesticides has been linked with both acute and chronic adverse health outcomes such as neurotoxicity and risk for certain cancers. Univariate analyses of pesticide exposures may not capture the complexity of these exposures since use of various pesticides often occurs simultaneously, and because specific uses have changed over time. Using data from the Agricultural Health Study, a cohort study of 89,658 licensed pesticide applicators and their spouses in Iowa and North Carolina, we employed factor analysis to order to characterize underlying patterns of self-reported exposures to 50 different pesticides. Factor analysis is a statistical method used to explain the relationships between several correlated variables by reducing them to a smaller number of conceptually meaningful, composite variables, known as factors. Three factors emerged for farmer applicators (N=45,074): (1) Iowa agriculture and herbicide use, (2) North Carolina agriculture and use of insecticides, fumigants and fungicides, and (3) older age and use of chlorinated pesticides. The patterns observed for spouses of farmers (N=17,488) were similar to those observed for the farmers themselves, whereas five factors emerged for commercial pesticide applicators (N=4,384): (1) herbicide use, (2) older age and use of chlorinated pesticides, (3) use of fungicides and residential pest treatments, (4) use of animal insecticides, and (5) use of fumigants. Pesticide exposures did not correlate with lifestyle characteristics such as race, smoking status or education. This heterogeneity in exposure patterns may be used to guide etiologic studies of health effects of farmers and other groups exposed to pesticides. JF - Journal of exposure analysis and environmental epidemiology AU - Samanic, Claudine AU - Hoppin, Jane A AU - Lubin, Jay H AU - Blair, Aaron AU - Alavanja, Michael C R AD - National Cancer Institute, Division of Cancer Epidemiology & Genetics, Bethesda, Maryland, USA. samanicc@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 225 EP - 233 VL - 15 IS - 3 SN - 1053-4245, 1053-4245 KW - Pesticides KW - 0 KW - Index Medicus KW - Life Style KW - Factor Analysis, Statistical KW - Age Factors KW - Humans KW - Cohort Studies KW - Adult KW - Middle Aged KW - Iowa KW - Male KW - Female KW - Spouses KW - Risk Assessment KW - Pesticides -- analysis KW - Occupational Exposure KW - Agriculture UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67821697?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+exposure+analysis+and+environmental+epidemiology&rft.atitle=Factor+analysis+of+pesticide+use+patterns+among+pesticide+applicators+in+the+Agricultural+Health+Study.&rft.au=Samanic%2C+Claudine%3BHoppin%2C+Jane+A%3BLubin%2C+Jay+H%3BBlair%2C+Aaron%3BAlavanja%2C+Michael+C+R&rft.aulast=Samanic&rft.aufirst=Claudine&rft.date=2005-05-01&rft.volume=15&rft.issue=3&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Journal+of+exposure+analysis+and+environmental+epidemiology&rft.issn=10534245&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-23 N1 - Date created - 2005-05-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The role of microglia in paraquat-induced dopaminergic neurotoxicity. AN - 67818527; 15890010 AB - The herbicide paraquat (PQ) has been implicated as a potential risk factor for the development of Parkinson's disease. In this study, PQ (0.5-1 microM) was shown to be selectively toxic to dopaminergic (DA) neurons through the activation of microglial NADPH oxidase and the generation of superoxide. Neuron-glia cultures exposed to PQ exhibited a decrease in DA uptake and a decline in the number of tyrosine hydroxylase-immunoreactive cells. The selectivity of PQ for DA neurons was confirmed when PQ failed to alter gamma-aminobutyric acid uptake in neuron-glia cultures. Microglia-depleted cultures exposed to 1 microM PQ failed to demonstrate a reduction in DA uptake, identifying microglia as the critical cell type mediating PQ neurotoxicity. Neuron-glia cultures treated with PQ failed to generate tumor necrosis factor-alpha and nitric oxide. However, microglia-enriched cultures exposed to PQ produced extracellular superoxide, supporting the notion that microglia are a source of PQ-derived oxidative stress. Neuron-glia cultures from NADPH oxidase-deficient (PHOX-/-) mice, which lack the functional catalytic subunit of NADPH oxidase and are unable to produce the respiratory burst, failed to show neurotoxicity in response to PQ, in contrast to PHOX+/+ mice. Here we report a novel mechanism of PQinduced oxidative stress, where at lower doses, the indirect insult generated from microglial NADPH oxidase is the essential factor mediating DA neurotoxicity. JF - Antioxidants & redox signaling AU - Wu, Xue-Fei AU - Block, Michelle L AU - Zhang, Wei AU - Qin, Liya AU - Wilson, Belinda AU - Zhang, Wan-Qin AU - Veronesi, Bellina AU - Hong, Jau-Shyong AD - Neuropharmacology Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA. PY - 2005 SP - 654 EP - 661 VL - 7 IS - 5-6 SN - 1523-0864, 1523-0864 KW - Superoxides KW - 11062-77-4 KW - NADPH Oxidase KW - EC 1.6.3.1 KW - Paraquat KW - PLG39H7695 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Rats KW - NADPH Oxidase -- metabolism KW - Animals KW - Superoxides -- metabolism KW - NADPH Oxidase -- deficiency KW - Cells, Cultured KW - Mice KW - Substrate Specificity KW - NADPH Oxidase -- genetics KW - Male KW - Female KW - Mice, Knockout KW - Microglia -- physiology KW - Microglia -- enzymology KW - Neurons -- metabolism KW - Neurons -- drug effects KW - Dopamine -- metabolism KW - Microglia -- drug effects KW - Paraquat -- toxicity KW - Neurons -- pathology KW - Microglia -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67818527?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antioxidants+%26+redox+signaling&rft.atitle=The+role+of+microglia+in+paraquat-induced+dopaminergic+neurotoxicity.&rft.au=Wu%2C+Xue-Fei%3BBlock%2C+Michelle+L%3BZhang%2C+Wei%3BQin%2C+Liya%3BWilson%2C+Belinda%3BZhang%2C+Wan-Qin%3BVeronesi%2C+Bellina%3BHong%2C+Jau-Shyong&rft.aulast=Wu&rft.aufirst=Xue-Fei&rft.date=2005-05-01&rft.volume=7&rft.issue=5-6&rft.spage=654&rft.isbn=&rft.btitle=&rft.title=Antioxidants+%26+redox+signaling&rft.issn=15230864&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-27 N1 - Date created - 2005-05-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Use of mycophenolate mofetil for chronic, refractory immune cytopenias in children with autoimmune lymphoproliferative syndrome. AN - 67805542; 15877736 AB - Autoimmune lymphoproliferative syndrome (ALPS) is a disorder of apoptosis associated most often with heritable FAS mutations leading to lymphadenopathy, hypersplenism and chronic refractory autoimmune cytopenias. Mycophenolate mofetil (MMF) was used to treat cytopenias in 13 ALPS patients aged 9 months to 17 years from a cohort of 118 children (aged < 18 years) and 82 adults. Twelve responded for a median follow-up of 49 weeks (range 38-240 weeks), defined by maintenance of adequate blood counts and reduction in dosage or cessation of other immunosuppressive agents. This preliminary experience suggests that MMF may spare steroid usage in patients with ALPS-associated cytopenias. JF - British journal of haematology AU - Rao, V Koneti AU - Dugan, Faith AU - Dale, Janet K AU - Davis, Joie AU - Tretler, Jean AU - Hurley, John K AU - Fleisher, Thomas AU - Puck, Jennifer AU - Straus, Stephen E AD - Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases/NIH, 10 Center Drive, Bethesda, MD 20892, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 534 EP - 538 VL - 129 IS - 4 SN - 0007-1048, 0007-1048 KW - Immunosuppressive Agents KW - 0 KW - Prodrugs KW - Mycophenolic Acid KW - HU9DX48N0T KW - Index Medicus KW - Thrombocytopenia -- drug therapy KW - Neutropenia -- immunology KW - Humans KW - Anemia, Hemolytic, Autoimmune -- drug therapy KW - Child KW - Leukocyte Count KW - Child, Preschool KW - Infant KW - Thrombocytopenia -- immunology KW - Pain -- chemically induced KW - Follow-Up Studies KW - Adolescent KW - Neutropenia -- drug therapy KW - Anemia, Hemolytic, Autoimmune -- immunology KW - Male KW - Lymphoproliferative Disorders -- drug therapy KW - Mycophenolic Acid -- analogs & derivatives KW - Mycophenolic Acid -- therapeutic use KW - Autoimmune Diseases -- drug therapy KW - Lymphoproliferative Disorders -- immunology KW - Mycophenolic Acid -- adverse effects KW - Immunosuppressive Agents -- therapeutic use KW - Immunosuppressive Agents -- adverse effects KW - Autoimmune Diseases -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67805542?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=&rft.atitle=Signaling+pathways+for+DNA+damage+and+repair%2C+apoptosis+and+lymphoid+progenitor+cell+survival+are+dysregulated+by+N-acetyl-L-cysteine&rft.au=French%2C+JE%3BMartin%2C+K+R%3BKari%2C+F+W%3BBarrett%2C+J+C&rft.aulast=French&rft.aufirst=JE&rft.date=2005-05-25&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=&rft.issn=&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-14 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neoadjuvant accelerated chemotherapy followed by hyperfractionated radiation therapy in patients with operable, locally advanced head and neck carcinoma. AN - 67804680; 15878759 AB - A prospective phase II trial was carried out to evaluate an accelerated chemotherapy (CT) regimen followed by hyperfractionated radiation therapy (RT) in previously untreated Stage III-IV, operable (total laryngectomy), head and neck cancer patients. The current study evaluates overall survival, loco-regional control, organ preservation rates and toxicity. Between April 1997 and December 2002, 68 patients with advanced head and neck cancer were treated with 3 cycles of induction CT (cisplatin and 5-fluorouracil; days 1, 14, and 28) followed by hyperfractionated RT (7440 cGy/62 fractions). Sixty patients received the planned RT-CT treatment. Two months after the end of RT, 96% of patients had a clinical complete remission of the primary and 66% of the neck disease. At a median follow-up of 32 months, the 3-year overall and disease-free survival rates were 66% and 76%, respectively. Seven patients recurred on the primary site, 1 on the neck and 2 patients only had distant metastases. The organ preservation rate was 73%. Acute grade 3-4 mucositis occurred in 75% of patients and an 18% rate of CT-related cardiotoxicity was reported. The accelerated CT-RT regimen achieves a high rate of larynx preservation albeit with considerable toxicity. The current prospective clinical trial was approved by the Ethics Committee of the Centro di Riferimento Oncologico (C.R.O.) on May 27, 1996, # CRO-02-96. Written informed consent was required from all patients entering the study. JF - Oral oncology AU - Franchin, Giovanni AU - Vaccher, Emanuela AU - Gobitti, Carlo AU - Minatel, Emilio AU - Politi, Doriano AU - Talamini, Renato AU - Di Gennaro, Giampiero AU - Savignano, Gabriella AU - Trovò, Mauro G AU - Tirelli, Umberto AU - Barzan, Luigi AD - Department of Radiation Oncology, Centro di Riferimento Oncologico, National Cancer Institute, 33081 Aviano, Italy. gfranchin@cro.it Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 526 EP - 533 VL - 41 IS - 5 SN - 1368-8375, 1368-8375 KW - Granulocyte Colony-Stimulating Factor KW - 143011-72-7 KW - Cisplatin KW - Q20Q21Q62J KW - Fluorouracil KW - U3P01618RT KW - Index Medicus KW - Humans KW - Mouth Mucosa -- radiation effects KW - Stomatitis -- etiology KW - Aged KW - Radiotherapy -- adverse effects KW - Cisplatin -- administration & dosage KW - Fluorouracil -- administration & dosage KW - Prospective Studies KW - Adult KW - Treatment Outcome KW - Granulocyte Colony-Stimulating Factor -- administration & dosage KW - Middle Aged KW - Chemotherapy, Adjuvant KW - Female KW - Male KW - Survival Analysis KW - Radiation Injuries -- etiology KW - Head and Neck Neoplasms -- radiotherapy KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Head and Neck Neoplasms -- drug therapy KW - Carcinoma, Squamous Cell -- radiotherapy KW - Carcinoma, Squamous Cell -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67804680?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Oral+oncology&rft.atitle=Neoadjuvant+accelerated+chemotherapy+followed+by+hyperfractionated+radiation+therapy+in+patients+with+operable%2C+locally+advanced+head+and+neck+carcinoma.&rft.au=Franchin%2C+Giovanni%3BVaccher%2C+Emanuela%3BGobitti%2C+Carlo%3BMinatel%2C+Emilio%3BPoliti%2C+Doriano%3BTalamini%2C+Renato%3BDi+Gennaro%2C+Giampiero%3BSavignano%2C+Gabriella%3BTrov%C3%B2%2C+Mauro+G%3BTirelli%2C+Umberto%3BBarzan%2C+Luigi&rft.aulast=Franchin&rft.aufirst=Giovanni&rft.date=2005-05-01&rft.volume=41&rft.issue=5&rft.spage=526&rft.isbn=&rft.btitle=&rft.title=Oral+oncology&rft.issn=13688375&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-05-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neuroprotection by the PGE2 EP2 receptor in permanent focal cerebral ischemia. AN - 67797641; 15852374 AB - Recent studies suggest a neuroprotective function of the PGE2 EP2 receptor in excitotoxic neuronal injury. The function of the EP2 receptor was examined at time points after excitotoxicity in an organotypic hippocampal model of N-methyl-D-aspartate (NMDA) challenge and in a permanent model of focal forebrain ischemia. Activation of EP2 led to significant neuroprotection in hippocampal slices up to 3 hours after a toxic NMDA stimulus. Genetic deletion of EP2 resulted in a marked increase in stroke volume in the permanent middle cerebral artery occlusion model. These findings support further investigation into therapeutic strategies targeting the EP2 receptor in stroke. JF - Annals of neurology AU - Liu, Dong AU - Wu, Liejun AU - Breyer, Richard AU - Mattson, Mark P AU - Andreasson, Katrin AD - Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, Baltimore, MD 21205, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 758 EP - 761 VL - 57 IS - 5 SN - 0364-5134, 0364-5134 KW - Excitatory Amino Acid Agonists KW - 0 KW - Ptger2 protein, mouse KW - Receptors, Prostaglandin E KW - Receptors, Prostaglandin E, EP2 Subtype KW - N-Methylaspartate KW - 6384-92-5 KW - Alprostadil KW - F5TD010360 KW - butaprost KW - HP16WVP23Y KW - Index Medicus KW - Animals KW - Cell Death -- physiology KW - Cerebral Infarction -- pathology KW - Mice KW - Mice, Knockout KW - Neurons -- pathology KW - Rats KW - Rats, Sprague-Dawley KW - N-Methylaspartate -- pharmacology KW - Mice, Inbred C57BL KW - Aging -- pathology KW - Excitatory Amino Acid Agonists -- pharmacology KW - Chronic Disease KW - Hippocampus -- pathology KW - Alprostadil -- pharmacology KW - Receptors, Prostaglandin E -- genetics KW - Brain Ischemia -- pathology KW - Alprostadil -- analogs & derivatives KW - Receptors, Prostaglandin E -- physiology KW - Receptors, Prostaglandin E -- agonists KW - Brain Ischemia -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67797641?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+neurology&rft.atitle=Neuroprotection+by+the+PGE2+EP2+receptor+in+permanent+focal+cerebral+ischemia.&rft.au=Liu%2C+Dong%3BWu%2C+Liejun%3BBreyer%2C+Richard%3BMattson%2C+Mark+P%3BAndreasson%2C+Katrin&rft.aulast=Liu&rft.aufirst=Dong&rft.date=2005-05-01&rft.volume=57&rft.issue=5&rft.spage=758&rft.isbn=&rft.btitle=&rft.title=Annals+of+neurology&rft.issn=03645134&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-24 N1 - Date created - 2005-05-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Evidence for novel cannabinoid receptors. AN - 67786836; 15866316 AB - Cannabinoids, including the bioactive constituents of the marijuana plant, their synthetic analogs, and endogenous lipids with cannabinoid-like activity, produce their biological effects by interacting with specific receptors. To date, two G protein-coupled cannabinoid receptors have been identified by molecular cloning, CB1 receptors mainly expressed in the brain and mediating most of the neurobehavioral effects of cannabinoids and CB2 receptors expressed by immune and hematopoietic tissues. Recent findings indicate that some cannabinoid effects are not mediated by either CB1 or CB2 receptors, and in some cases there is compelling evidence to implicate additional receptors in these actions. These include transient receptor potential vanilloid 1 (TRPV1) receptors and as-yet-unidentified receptors implicated in the endothelium-dependent vasodilator effect of certain cannabinoids and in the presynaptic inhibition of glutamatergic neurotransmission in the hippocampus. The case for these additional receptors is being reviewed here. JF - Pharmacology & therapeutics AU - Begg, Malcolm AU - Pacher, Pál AU - Bátkai, Sándor AU - Osei-Hyiaman, Douglas AU - Offertáler, László AU - Mo, Fong Ming AU - Liu, Jie AU - Kunos, George AD - National Institute of Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, MSC-9413 Bethesda, MD 8092-9413, United States. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 133 EP - 145 VL - 106 IS - 2 SN - 0163-7258, 0163-7258 KW - Cannabinoid Receptor Antagonists KW - 0 KW - Cannabinoids KW - Ion Channels KW - Piperidines KW - Pyrazoles KW - Receptors, Cannabinoid KW - TRPV Cation Channels KW - TRPV1 protein, human KW - rimonabant KW - RML78EN3XE KW - Index Medicus KW - Piperidines -- pharmacology KW - Pyrazoles -- pharmacology KW - Animals KW - Drug Interactions KW - Ion Channels -- pharmacology KW - Humans KW - Receptors, Cannabinoid -- physiology KW - Brain -- drug effects KW - Receptors, Cannabinoid -- drug effects KW - Brain -- metabolism KW - Cannabinoids -- pharmacology KW - Cannabinoids -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786836?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacology+%26+therapeutics&rft.atitle=Evidence+for+novel+cannabinoid+receptors.&rft.au=Begg%2C+Malcolm%3BPacher%2C+P%C3%A1l%3BB%C3%A1tkai%2C+S%C3%A1ndor%3BOsei-Hyiaman%2C+Douglas%3BOffert%C3%A1ler%2C+L%C3%A1szl%C3%B3%3BMo%2C+Fong+Ming%3BLiu%2C+Jie%3BKunos%2C+George&rft.aulast=Begg&rft.aufirst=Malcolm&rft.date=2005-05-01&rft.volume=106&rft.issue=2&rft.spage=133&rft.isbn=&rft.btitle=&rft.title=Pharmacology+%26+therapeutics&rft.issn=01637258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-30 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Identification of plasma membrane macro- and microdomains from wavelet analysis of FRET microscopy. AN - 67786764; 15722423 AB - In this study, we sought to characterize functional signaling domains by applying the multiresolution properties of the continuous wavelet transform to fluorescence resonance energy transfer (FRET) microscopic images of plasma membranes. A genetically encoded FRET reporter of protein kinase C (PKC)-dependent phosphorylation was expressed in COS1 cells. Differences between wavelet coefficient matrices revealed several heterogeneous domains (typically ranging from 1 to 5 microm), reflecting the dynamic balance between PKC and phosphatase activity during stimulation with phorbol-12,13-dibutyrate or acetylcholine. The balance in these domains was not necessarily reflected in the overall plasma membrane changes, and observed heterogeneity was absent when cells were exposed to a phosphatase or PKC inhibitor. Prolonged exposure to phorbol-12,13-dibutyrate and acetylcholine yielded more homogeneous FRET distribution in plasma membranes. The proposed wavelet-based image analysis provides, for the first time, a basis and a means of detecting and quantifying dynamic changes in functional signaling domains, and may find broader application in studying fine aspects of cellular signaling by various imaging reporters. JF - Biophysical journal AU - Kobrinsky, Evgeny AU - Mager, Donald E AU - Bentil, Sarah A AU - Murata, Shin-Ichi AU - Abernethy, Darrell R AU - Soldatov, Nikolai M AD - National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3625 EP - 3634 VL - 88 IS - 5 SN - 0006-3495, 0006-3495 KW - Adaptor Proteins, Signal Transducing KW - 0 KW - Bacterial Proteins KW - Cyan Fluorescent Protein KW - Luminescent Proteins KW - yellow fluorescent protein, Bacteria KW - Green Fluorescent Proteins KW - 147336-22-9 KW - Phorbol 12,13-Dibutyrate KW - 37558-16-0 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Phosphoric Monoester Hydrolases KW - EC 3.1.3.2 KW - Index Medicus KW - Animals KW - Green Fluorescent Proteins -- chemistry KW - COS Cells KW - Protein Kinase C -- genetics KW - Models, Statistical KW - Luminescent Proteins -- chemistry KW - Phosphoric Monoester Hydrolases -- metabolism KW - Adaptor Proteins, Signal Transducing -- chemistry KW - Phorbol 12,13-Dibutyrate -- chemistry KW - Bacterial Proteins -- chemistry KW - Phosphorylation KW - Protein Kinase C -- chemistry KW - Genes, Reporter KW - Protein Structure, Tertiary KW - Time Factors KW - Image Processing, Computer-Assisted KW - Signal Transduction KW - Cell Membrane -- metabolism KW - Fluorescence Resonance Energy Transfer -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786764?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biophysical+journal&rft.atitle=Identification+of+plasma+membrane+macro-+and+microdomains+from+wavelet+analysis+of+FRET+microscopy.&rft.au=Kobrinsky%2C+Evgeny%3BMager%2C+Donald+E%3BBentil%2C+Sarah+A%3BMurata%2C+Shin-Ichi%3BAbernethy%2C+Darrell+R%3BSoldatov%2C+Nikolai+M&rft.aulast=Kobrinsky&rft.aufirst=Evgeny&rft.date=2005-05-01&rft.volume=88&rft.issue=5&rft.spage=3625&rft.isbn=&rft.btitle=&rft.title=Biophysical+journal&rft.issn=00063495&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-18 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biochem. 2002 Nov;132(5):663-8 [12417013] Curr Opin Cell Biol. 2002 Aug;14(4):483-7 [12383800] J Cell Biol. 2003 Jun 9;161(5):899-909 [12782683] J Histochem Cytochem. 2003 Jul;51(7):951-8 [12810845] Biophys J. 2003 Jul;85(1):559-71 [12829510] Biophys J. 2003 Oct;85(4):2170-85 [14507683] Cell. 2004 Feb 20;116(4):577-89 [14980224] Nat Med. 2004 Mar;10(3):248-54 [14966518] Circ Res. 2004 Apr 16;94(7):866-73 [15087426] Biophys J. 2004 Aug;87(2):844-57 [15298893] J Cell Sci. 2004 Nov 1;117(Pt 23):5521-34 [15479718] Biophys J. 1993 Sep;65(3):1135-46 [8241393] J Microsc. 2004 Nov;216(Pt 2):110-22 [15516222] J Microsc. 2004 May;214(Pt 2):190-200 [15102066] Cytometry. 2001 Feb 1;43(2):94-100 [11169573] Methods. 2001 Jul;24(3):289-96 [11403577] Biophys J. 2001 Oct;81(4):2395-402 [11566809] Annu Rev Biomed Eng. 2000;2:511-50 [11701522] Science. 2002 May 3;296(5569):913-6 [11988576] J Biol Chem. 2003 Feb 14;278(7):5021-8 [12473653] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - How can drug addiction help us understand obesity? AN - 67786146; 15856062 JF - Nature neuroscience AU - Volkow, Nora D AU - Wise, Roy A AD - National Institute on Drug Abuse, National Institutes of Health, Bethesda, Maryland 20892, USA. nvolkow@nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 555 EP - 560 VL - 8 IS - 5 SN - 1097-6256, 1097-6256 KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Neural Pathways -- physiopathology KW - Animals KW - Adaptation, Physiological -- physiology KW - Humans KW - Reinforcement (Psychology) KW - Neural Pathways -- physiology KW - Appetite Regulation -- physiology KW - Dopamine -- metabolism KW - Substance-Related Disorders -- physiopathology KW - Brain -- physiopathology KW - Behavior, Addictive -- psychology KW - Obesity -- psychology KW - Behavior, Addictive -- physiopathology KW - Substance-Related Disorders -- psychology KW - Obesity -- physiopathology KW - Brain -- growth & development UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67786146?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature&rft.atitle=Race+against+time.&rft.au=Fauci%2C+Anthony+S&rft.aulast=Fauci&rft.aufirst=Anthony&rft.date=2005-05-26&rft.volume=435&rft.issue=7041&rft.spage=423&rft.isbn=&rft.btitle=&rft.title=Nature&rft.issn=1476-4687&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-08 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Efficacy, plasma pharmacokinetics, and safety of icofungipen, an inhibitor of Candida isoleucyl-tRNA synthetase, in treatment of experimental disseminated candidiasis in persistently neutropenic rabbits. AN - 67784556; 15855534 AB - Icofungipen (formerly PLD-118) is a synthetic derivative of the naturally occurring beta-amino acid cispentacin that blocks isoleucyl-tRNA synthetase, resulting in the inhibition of protein synthesis and growth of fungal cells. We investigated the efficacy, plasma pharmacokinetics, and safety of icofungipen in escalating dosages for the treatment of experimental subacute disseminated candidiasis in persistently neutropenic rabbits. Icofungipen was administered for 10 days starting 24 h after the intravenous inoculation of 10(3) Candida albicans blastoconidia. Study groups consisted of rabbits treated with icofungipen at 4 (ICO-4), 10 (ICO-10), and 25 (ICO-25) mg/kg of body weight/day in two divided dosages, rabbits treated with fluconazole at 10 mg/kg/day, rabbits treated with amphotericin B at 1 mg/kg/day, and untreated controls. Levels of icofungipen in plasma were derivatized by phthaldialdehyde and quantified by high-performance liquid chromatography with fluorescence detection. Rabbits treated with ICO-10 (P < 0.01) and ICO-25 (P < 0.001) showed significant dosage-dependent tissue clearance of C. albicans from the liver, spleen, kidney, brain, vitreous, vena cava, and lung in comparison to untreated controls. ICO-25 cleared C. albicans from all tissues and had activity comparable to that of amphotericin B versus untreated controls (P < 0.001). Pharmacokinetics of icofungipen in plasma approximated a dose-dependent relationship of the maximum concentration of drug in serum and the area under the concentration-time curve. There was no significant elevation of the levels of hepatic transaminases, alkaline phosphatase, bilirubin, urea nitrogen, or creatinine in icofungipen-treated rabbits. Icofungipen followed dose-dependent pharmacokinetics and was effective in the treatment of experimental disseminated candidiasis, including central nervous system infection, in persistently neutropenic rabbits. JF - Antimicrobial agents and chemotherapy AU - Petraitiene, Ruta AU - Petraitis, Vidmantas AU - Kelaher, Amy M AU - Sarafandi, Alia A AU - Mickiene, Diana AU - Groll, Andreas H AU - Sein, Tin AU - Bacher, John AU - Walsh, Thomas J AD - Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892-1100, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2084 EP - 2092 VL - 49 IS - 5 SN - 0066-4804, 0066-4804 KW - 2-amino-4-methylenecyclopentane-1-carboxylic acid KW - 0 KW - Antifungal Agents KW - Culture Media KW - Cycloleucine KW - 0TQU7668EI KW - Isoleucine-tRNA Ligase KW - EC 6.1.1.5 KW - Index Medicus KW - Animals KW - Spleen -- microbiology KW - Rabbits KW - Liver -- microbiology KW - Female KW - Kidney -- microbiology KW - Immunosuppression KW - Cycloleucine -- pharmacokinetics KW - Antifungal Agents -- adverse effects KW - Antifungal Agents -- pharmacokinetics KW - Cycloleucine -- pharmacology KW - Neutropenia -- complications KW - Candida albicans -- ultrastructure KW - Cycloleucine -- adverse effects KW - Cycloleucine -- analogs & derivatives KW - Candida albicans -- enzymology KW - Candidiasis -- microbiology KW - Candidiasis -- drug therapy KW - Antifungal Agents -- pharmacology KW - Isoleucine-tRNA Ligase -- antagonists & inhibitors KW - Candida albicans -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784556?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+agents+and+chemotherapy&rft.atitle=Efficacy%2C+plasma+pharmacokinetics%2C+and+safety+of+icofungipen%2C+an+inhibitor+of+Candida+isoleucyl-tRNA+synthetase%2C+in+treatment+of+experimental+disseminated+candidiasis+in+persistently+neutropenic+rabbits.&rft.au=Petraitiene%2C+Ruta%3BPetraitis%2C+Vidmantas%3BKelaher%2C+Amy+M%3BSarafandi%2C+Alia+A%3BMickiene%2C+Diana%3BGroll%2C+Andreas+H%3BSein%2C+Tin%3BBacher%2C+John%3BWalsh%2C+Thomas+J&rft.aulast=Petraitiene&rft.aufirst=Ruta&rft.date=2005-05-01&rft.volume=49&rft.issue=5&rft.spage=2084&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+agents+and+chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-14 N1 - Date created - 2005-04-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Trends Microbiol. 2000 May;8(5):200-1 [10785633] Clin Infect Dis. 1999 Aug;29(2):239-44 [10476719] Clin Infect Dis. 2001 Jun 15;32(12):1713-7 [11360213] Expert Opin Investig Drugs. 2001 Aug;10(8):1545-58 [11772269] Clin Infect Dis. 2002 Mar 1;34(5):600-2 [11810604] J Clin Microbiol. 2002 Apr;40(4):1298-302 [11923348] Antimicrob Agents Chemother. 2002 Jun;46(6):1857-69 [12019101] J Antimicrob Chemother. 2002 Jun;49(6):889-91 [12039879] J Clin Microbiol. 2002 Oct;40(10):3551-7 [12354845] N Engl J Med. 2002 Dec 19;347(25):2070-2 [12490691] Drug Discov Today. 2002 Mar 15;7(6):332-3 [11893534] Infect Dis Clin North Am. 2002 Dec;16(4):821-35 [12512183] Bioorg Med Chem Lett. 2003 Feb 10;13(3):433-6 [12565945] N Engl J Med. 2003 Apr 17;348(16):1546-54 [12700374] Expert Opin Pharmacother. 2003 May;4(5):807-23 [12740003] Clin Infect Dis. 2003 Sep 1;37(5):634-43 [12942393] Pediatrics. 2003 Sep;112(3 Pt 1):543-7 [12949281] Drug Resist Updat. 2003 Aug;6(4):197-218 [12962685] Lancet. 2003 Oct 4;362(9390):1142-51 [14550704] J Hosp Infect. 2003 Nov;55(3):159-68; quiz 233 [14572481] Clin Infect Dis. 2004 Apr 15;38(8):1119-27 [15095217] Pediatr Infect Dis J. 2004 Jul;23(7):635-41 [15247602] J Clin Microbiol. 1987 May;25(5):931-2 [3294892] Lab Anim Sci. 1988 Aug;38(4):467-71 [3184859] J Clin Microbiol. 1990 Jul;28(7):1616-22 [2380383] Antimicrob Agents Chemother. 1991 Jul;35(7):1321-8 [1929288] Can J Microbiol. 1991 Aug;37(8):637-46 [1954577] Antimicrob Agents Chemother. 1995 Jan;39(1):1-8 [7695288] Clin Infect Dis. 1995 Jun;20(6):1526-30 [7548503] Antimicrob Agents Chemother. 1997 Jun;41(6):1392-5 [9174207] Antimicrob Agents Chemother. 1998 May;42(5):1207-12 [9593151] Antimicrob Agents Chemother. 1998 Jul;42(7):1581-6 [9660987] Antimicrob Agents Chemother. 1998 Sep;42(9):2197-205 [9736535] Am J Health Syst Pharm. 1999 Mar 15;56(6):525-33; quiz 534-5 [10192687] Antimicrob Agents Chemother. 1999 Sep;43(9):2148-55 [10471556] Braz J Infect Dis. 2000 Aug;4(4):161-7 [11008219] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Dietary supplements in weight reduction. AN - 67784457; 15867902 AB - We summarize evidence on the role of dietary supplements in weight reduction, with particular attention to their safety and benefits. Dietary supplements are used for two purposes in weight reduction: (a) providing nutrients that may be inadequate in calorie-restricted diets and (b) for their potential benefits in stimulating weight loss. The goal in planning weight-reduction diets is that total intake from food and supplements should meet recommended dietary allowance/adequate intake levels without greatly exceeding them for all nutrients, except energy. If nutrient amounts from food sources in the reducing diet fall short, dietary supplements containing a single nutrient/element or a multivitamin-mineral combination may be helpful. On hypocaloric diets, the addition of dietary supplements providing nutrients at a level equal to or below recommended dietary allowance/adequate intake levels or 100% daily value, as stated in a supplement's facts box on the label, may help dieters to achieve nutrient adequacy and maintain electrolyte balance while avoiding the risk of excessive nutrient intakes. Many botanical and other types of dietary supplements are purported to be useful for stimulating or enhancing weight loss. Evidence of their efficacy in stimulating weight loss is inconclusive at present. Although there are few examples of safety concerns related to products that are legal and on the market for this purpose, there is also a paucity of evidence on safety for this intended use. Ephedra and ephedrine-containing supplements, with or without caffeine, have been singled out in recent alerts from the Food and Drug Administration because of safety concerns, and use of products containing these substances cannot be recommended. Dietitians should periodically check the Food and Drug Administration Web site ( www.cfsan.fda.gov ) for updates and warnings and alert patients/clients to safety concerns. Dietetics professionals should also consult authoritative sources for new data on efficacy as it becomes available ( ods.od.nih.gov ). JF - Journal of the American Dietetic Association AU - Dwyer, Johanna T AU - Allison, David B AU - Coates, Paul M AD - Office of Dietary Supplements, National Institutes of Health, Bethesda, Maryland 20892, USA. DwyerJ1@od.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - S80 EP - S86 VL - 105 IS - 5 Suppl 1 SN - 0002-8223, 0002-8223 KW - Anti-Obesity Agents KW - 0 KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Nutritional Requirements KW - United States Food and Drug Administration KW - Consumer Product Safety KW - Humans KW - Safety KW - Nutrition Policy KW - Treatment Outcome KW - Caloric Restriction KW - Obesity -- drug therapy KW - Weight Loss -- drug effects KW - Dietary Supplements KW - Anti-Obesity Agents -- adverse effects KW - Anti-Obesity Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784457?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=World+journal+of+gastroenterology&rft.atitle=Mismatch+repair+genes+%28hMLH1%2C+hPMS1%2C+hPMS2%2C+GTBP%2FhMSH6%2C+hMSH2%29+in+the+pathogenesis+of+hepatocellular+carcinoma.&rft.au=Zekri%2C+Abdel-Rahman+N%3BSabry%2C+Gelane+M%3BBahnassy%2C+Abeer+A%3BShalaby%2C+Kamal+A%3BAbdel-Wahabh%2C+Sabrin+A%3BZakaria%2C+Serag&rft.aulast=Zekri&rft.aufirst=Abdel-Rahman&rft.date=2005-05-28&rft.volume=11&rft.issue=20&rft.spage=3020&rft.isbn=&rft.btitle=&rft.title=World+journal+of+gastroenterology&rft.issn=10079327&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-28 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Combining a recombinant cancer vaccine with standard definitive radiotherapy in patients with localized prostate cancer. AN - 67784255; 15867235 AB - Many patients with clinically localized prostate cancer develop biochemical failure despite excellent local therapy perhaps due to occult metastatic disease. One potential solution is the utilization of a well-tolerated systemic therapy (e.g., vaccine) in concert with local therapy. We present a randomized phase II clinical trial designed to determine if a poxviral vaccine encoding prostate-specific antigen (PSA) can induce a PSA-specific T-cell response when combined with radiotherapy in patients with clinically localized prostate cancer. Thirty patients were randomized in a 2:1 ratio into vaccine plus radiotherapy or radiotherapy-only arms. Those patients in the combination arm received a "priming" vaccine with recombinant vaccinia (rV) PSA plus r V containing the T-cell costimulatory molecule B7.1 (rV-B7.1) followed by monthly booster vaccines with recombinant fowlpox PSA. The vaccines were given with local granulocyte-macrophage colony-stimulating factor and low-dose systemic interleukin-2. Standard external beam radiation therapy was given between the fourth and the sixth vaccinations. Seventeen of 19 patients in the combination arm completed all eight vaccinations and 13 of these 17 patients had increases in PSA-specific T cells of at least 3-fold versus no detectable increases in the radiotherapy-only arm (P < 0.0005). There was also evidence of de novo generation of T cells to well-described prostate-associated antigens not found in the vaccine, providing indirect evidence of immune-mediated tumor killing. The vaccine was well tolerated. This vaccine regimen can be safely given in patients undergoing radiation therapy for localized prostate cancer, with the majority of patients generating a PSA-specific cellular immune response to vaccine. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Gulley, James L AU - Arlen, Philip M AU - Bastian, Anne AU - Morin, Steven AU - Marte, Jennifer AU - Beetham, Patricia AU - Tsang, Kwong-Yok AU - Yokokawa, Junko AU - Hodge, James W AU - Ménard, Cynthia AU - Camphausen, Kevin AU - Coleman, C Norman AU - Sullivan, Francis AU - Steinberg, Seth M AU - Schlom, Jeffrey AU - Dahut, William AD - Laboratory of Tumor Immunology and Biology, Medical Oncology Clinical Research Unit, Radiation Oncology Branch, and Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 3353 EP - 3362 VL - 11 IS - 9 SN - 1078-0432, 1078-0432 KW - Antigens, CD80 KW - 0 KW - Cancer Vaccines KW - Recombinant Fusion Proteins KW - Prostate-Specific Antigen KW - EC 3.4.21.77 KW - Index Medicus KW - Recombinant Fusion Proteins -- immunology KW - Combined Modality Therapy KW - Humans KW - Antigens, CD80 -- genetics KW - Aged KW - Cell Line, Tumor KW - Cytotoxicity, Immunologic -- immunology KW - Antigens, CD80 -- immunology KW - Tumor Cells, Cultured KW - Aged, 80 and over KW - Prostate-Specific Antigen -- blood KW - Treatment Outcome KW - Cytotoxicity Tests, Immunologic KW - Middle Aged KW - Recombinant Fusion Proteins -- therapeutic use KW - Cell Line KW - Male KW - Prostatic Neoplasms -- immunology KW - Cancer Vaccines -- immunology KW - Cancer Vaccines -- adverse effects KW - Cancer Vaccines -- therapeutic use KW - Prostatic Neoplasms -- therapy KW - Prostatic Neoplasms -- radiotherapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Combining+a+recombinant+cancer+vaccine+with+standard+definitive+radiotherapy+in+patients+with+localized+prostate+cancer.&rft.au=Gulley%2C+James+L%3BArlen%2C+Philip+M%3BBastian%2C+Anne%3BMorin%2C+Steven%3BMarte%2C+Jennifer%3BBeetham%2C+Patricia%3BTsang%2C+Kwong-Yok%3BYokokawa%2C+Junko%3BHodge%2C+James+W%3BM%C3%A9nard%2C+Cynthia%3BCamphausen%2C+Kevin%3BColeman%2C+C+Norman%3BSullivan%2C+Francis%3BSteinberg%2C+Seth+M%3BSchlom%2C+Jeffrey%3BDahut%2C+William&rft.aulast=Gulley&rft.aufirst=James&rft.date=2005-05-01&rft.volume=11&rft.issue=9&rft.spage=3353&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-01 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):6757-62 [16203760] Erratum In: Clin Cancer Res. 2006 Jan 1;12(1):322 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Supra-additive growth inhibition by a celecoxib analogue and carboxyamido-triazole is primarily mediated through apoptosis. AN - 67783362; 15867384 AB - Combination studies of celecoxib and chemotherapeutic agents suggest that combining cyclooxygenase-2 inhibitors with other agents may have supra-additive or synergistic effects on tumor growth inhibition. Carboxyamido-triazole (CAI), a voltage-independent calcium channel inhibitor, has been shown to induce growth inhibition and apoptosis in cancer cells. We found that continuous exposure to cytostatic doses of CAI and LM-1685, a celecoxib analogue, reduced the proliferation and survival of seven human cancer cell lines by at least one log (P < or = 0.001) over either agent alone. To explore the mechanism of action of this combination, we further studied the effects of LM-1685/CAI on CCL-250 colorectal carcinoma cells. We found that the supra-additive antiproliferative effects occurred throughout a range of LM-1685 doses (5-25 micromol/L) and paralleled a decrease in COX-2 activity as measured by prostaglandin E2 production. In these cells, treatment with LM-1685/CAI suppressed the extracellular signal-regulated kinase pathway within the first hour but ultimately results in high, sustained activation of ERK over a 9-day period (P = 0.0005). Suppression of cyclin D1 and phospho-AKT, and cleavage of caspase-3 and PARP were concomitant with persistent ERK activation. Addition of PD98059, a MEK-1 inhibitor, suppressed ERK activation and significantly but incompletely reversed these signaling events and apoptosis. Flow cytometry experiments revealed that the CAI/LM-1685 combination induced a 3-fold increase in apoptosis over control (P = 0.005) in 3 days. We show that the combination of CAI and LM-1685 produces a cytotoxic effect by suppressing proliferation and triggering apoptosis. JF - Cancer research AU - Winters, Mary E AU - Mehta, Arpita I AU - Petricoin, Emanuel F AU - Kohn, Elise C AU - Liotta, Lance A AD - Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 3853 EP - 3860 VL - 65 IS - 9 SN - 0008-5472, 0008-5472 KW - Calcium Channel Blockers KW - 0 KW - Cyclooxygenase 2 Inhibitors KW - Cyclooxygenase Inhibitors KW - Indoles KW - LM-1685 KW - Membrane Proteins KW - Pyrazoles KW - Sulfonamides KW - Triazoles KW - carboxyamido-triazole KW - 99519-84-3 KW - Cyclooxygenase 2 KW - EC 1.14.99.1 KW - PTGS2 protein, human KW - Prostaglandin-Endoperoxide Synthases KW - Receptor, Epidermal Growth Factor KW - EC 2.7.10.1 KW - Celecoxib KW - JCX84Q7J1L KW - Dinoprostone KW - K7Q1JQR04M KW - Index Medicus KW - Pyrazoles -- administration & dosage KW - Drug Screening Assays, Antitumor KW - Dinoprostone -- pharmacology KW - Humans KW - Transcriptional Activation -- drug effects KW - Cell Line, Tumor KW - Sulfonamides -- administration & dosage KW - Prostaglandin-Endoperoxide Synthases -- metabolism KW - Signal Transduction -- drug effects KW - Drug Synergism KW - Cell Cycle -- drug effects KW - Calcium Channel Blockers -- pharmacology KW - Cyclooxygenase Inhibitors -- administration & dosage KW - Apoptosis -- drug effects KW - Calcium Channel Blockers -- administration & dosage KW - Indoles -- pharmacology KW - Antineoplastic Combined Chemotherapy Protocols -- pharmacology KW - Triazoles -- pharmacology KW - Triazoles -- administration & dosage KW - Cyclooxygenase Inhibitors -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783362?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Supra-additive+growth+inhibition+by+a+celecoxib+analogue+and+carboxyamido-triazole+is+primarily+mediated+through+apoptosis.&rft.au=Winters%2C+Mary+E%3BMehta%2C+Arpita+I%3BPetricoin%2C+Emanuel+F%3BKohn%2C+Elise+C%3BLiotta%2C+Lance+A&rft.aulast=Winters&rft.aufirst=Mary&rft.date=2005-05-01&rft.volume=65&rft.issue=9&rft.spage=3853&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-15 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Overview of aerosolized Florida red tide toxins: exposures and effects. AN - 67783255; 15866773 AB - Florida red tide is caused by Karenia brevis, a dinoflagellate that periodically blooms, releasing its potent neurotoxin, brevetoxin, into the surrounding waters and air along the coast of the Gulf of Mexico. Exposure to Florida red tide toxins has been associated with adverse human health effects and massive fish and marine mammal deaths. The articles in this mini-monograph describe the ongoing interdisciplinary and interagency research program that characterizes the exposures and health effects of aerosolized Florida red tide toxins (brevetoxins). The interdisciplinary research program uses animal models and laboratory studies to develop hypotheses and apply these findings to in situ human exposures. Our ultimate goal is to develop appropriate prevention measures and medical interventions to mitigate or prevent adverse health effects from exposure to complex mixtures of aerosolized red tide toxins. JF - Environmental health perspectives AU - Fleming, Lora E AU - Backer, Lorraine C AU - Baden, Daniel G AD - National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Sciences Center, University of Miami Rosenstiel School of Marine and Atmospheric Sciences, Miami, FL 33136, USA. lfleming@med.miami.edu Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 618 EP - 620 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Aerosols KW - 0 KW - Marine Toxins KW - Oxocins KW - brevetoxin KW - 98225-48-0 KW - Index Medicus KW - Environmental Monitoring KW - Animals KW - Eutrophication KW - Humans KW - Research -- trends KW - Florida KW - Public Health KW - Oxocins -- adverse effects KW - Dinoflagellida -- pathogenicity KW - Marine Toxins -- adverse effects KW - Environmental Exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Overview+of+aerosolized+Florida+red+tide+toxins%3A+exposures+and+effects.&rft.au=Fleming%2C+Lora+E%3BBacker%2C+Lorraine+C%3BBaden%2C+Daniel+G&rft.aulast=Fleming&rft.aufirst=Lora&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=618&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 2004 Jun;112(8):A455-6 [15175187] Environ Health Perspect. 2004 Jun;112(8):A454-5 [15175186] Environ Lett. 1972;3(4):271-8 [4627989] JFMA. 1973 Nov;60(11):27-9 [4755462] Int Rev Cytol. 1983;82:99-150 [6352551] Mol Pharmacol. 1991 Dec;40(6):988-94 [1661842] Nat Toxins. 1999;7(2):45-8 [10495465] Environ Health Perspect. 2005 May;113(5):621-5 [15866774] Nat Toxins. 1994;2(4):212-21 [7952946] Indian J Exp Biol. 1997 Jun;35(6):650-4 [9357171] Toxicol Pathol. 1998 Mar-Apr;26(2):276-82 [9547868] J Toxicol Environ Health A. 1999 Jul 9;57(5):345-55 [10405188] Environ Health Perspect. 2005 May;113(5):626-31 [15866775] Environ Health Perspect. 2005 May;113(5):632-7 [15866776] Environ Health Perspect. 2005 May;113(5):638-43 [15866777] Environ Health Perspect. 2005 May;113(5):644-9 [15866778] Environ Health Perspect. 2005 May;113(5):650-7 [15866779] J Soc Biol. 1999;193(3):329-44 [10542966] Neurotoxicology. 1999 Dec;20(6):909-20 [10693972] Environ Health Perspect. 2000 Mar;108 Suppl 1:133-41 [10698729] Toxicon. 2000 Jul;38(7):981-93 [10728835] Chem Biol. 2000 Jun;7(6):385-93 [10873835] Environ Toxicol Chem. 2001 Jan;20(1):107-14 [11351396] Environ Health Perspect. 2002 Feb;110(2):179-85 [11836147] Environ Health Perspect. 2002 Sep;110(9):839-45 [12204815] Cell Mol Neurobiol. 2004 Aug;24(4):553-63 [15233378] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Scientific vision: setting forth a strategy. AN - 67783162; 15866748 JF - Environmental health perspectives AU - Schwartz, David A AD - National Institute of Environmental Health Sciences/NIH, Durham, NC, USA. schwartzd@niehs.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Environmental Pollutants KW - 0 KW - Index Medicus KW - United States KW - Humans KW - Environmental Pollutants -- poisoning KW - Risk Assessment KW - Research Support as Topic KW - Environmental Health KW - National Institutes of Health (U.S.) -- organization & administration KW - Environmental Exposure KW - Organizational Objectives UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67783162?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Scientific+vision%3A+setting+forth+a+strategy.&rft.au=Schwartz%2C+David+A&rft.aulast=Schwartz&rft.aufirst=David&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=A292&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - IQ and blood lead from 2 to 7 years of age: are the effects in older children the residual of high blood lead concentrations in 2-year-olds? AN - 67780351; 15866769 AB - Increases in peak blood lead concentrations, which occur at 18-30 months of age in the United States, are thought to result in lower IQ scores at 4-6 years of age, when IQ becomes stable and measurable. Data from a prospective study conducted in Boston suggested that blood lead concentrations at 2 years of age were more predictive of cognitive deficits in older children than were later blood lead concentrations or blood lead concentrations measured concurrently with IQ. Therefore, cross-sectional associations between blood lead and IQ in school-age children have been widely interpreted as the residual effects of higher blood lead concentrations at an earlier age or the tendency of less intelligent children to ingest more leaded dust or paint chips, rather than as a causal relationship in older children. Here we analyze data from a clinical trial in which children were treated for elevated blood lead concentrations (20-44 microg/dL) at about 2 years of age and followed until 7 years of age with serial IQ tests and measurements of blood lead. We found that cross-sectional associations increased in strength as the children became older, whereas the relation between baseline blood lead and IQ attenuated. Peak blood lead level thus does not fully account for the observed association in older children between their lower blood lead concentrations and IQ. The effect of concurrent blood level on IQ may therefore be greater than currently believed. JF - Environmental health perspectives AU - Chen, Aimin AU - Dietrich, Kim N AU - Ware, James H AU - Radcliffe, Jerilynn AU - Rogan, Walter J AD - Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health/DHHS, Research Triangle Park, NC 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 597 EP - 601 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Chelating Agents KW - 0 KW - Placebos KW - Lead KW - 2P299V784P KW - Succimer KW - DX1U2629QE KW - Index Medicus KW - Intelligence KW - Cross-Sectional Studies KW - Age Factors KW - Humans KW - Confounding Factors (Epidemiology) KW - Child Development KW - Child KW - Male KW - Female KW - Child, Preschool KW - Cognition Disorders -- etiology KW - Lead Poisoning -- drug therapy KW - Chelating Agents -- therapeutic use KW - Succimer -- therapeutic use KW - Lead Poisoning -- complications KW - Lead -- blood UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67780351?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=IQ+and+blood+lead+from+2+to+7+years+of+age%3A+are+the+effects+in+older+children+the+residual+of+high+blood+lead+concentrations+in+2-year-olds%3F&rft.au=Chen%2C+Aimin%3BDietrich%2C+Kim+N%3BWare%2C+James+H%3BRadcliffe%2C+Jerilynn%3BRogan%2C+Walter+J&rft.aulast=Chen&rft.aufirst=Aimin&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=597&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Environ Health Perspect. 1997 Sep;105(9):956-62 [9410739] BMJ. 1996 Jun 22;312(7046):1569-75 [8664666] Paediatr Perinat Epidemiol. 1998 Jul;12(3):313-33 [9690266] Environ Health Perspect. 1998 Nov;106(11):745-50 [9799191] JAMA. 1998 Dec 9;280(22):1915-9 [9851476] Pediatr Res. 2000 Nov;48(5):593-9 [11044477] Neurotoxicol Teratol. 2000 Nov-Dec;22(6):811-8 [11120386] N Engl J Med. 2001 May 10;344(19):1421-6 [11346806] Public Health Rep. 2000 Nov-Dec;115(6):521-9 [11354334] Am J Epidemiol. 2001 Oct 15;154(8):711-7 [11590083] Pediatrics. 2002 Oct;110(4):787-91 [12359796] N Engl J Med. 2003 Apr 17;348(16):1517-26 [12700371] Pediatrics. 2004 Jul;114(1):19-26 [15231903] J Clin Psychol. 1985 Jan;41(1):95-7 [3973047] Analyst. 1987 Dec;112(12):1701-4 [3445938] Pediatrics. 1992 Dec;90(6):855-61 [1437425] JAMA. 1993 Apr 7;269(13):1641-6 [8455297] Neurotoxicol Teratol. 1993 Jan-Feb;15(1):37-44 [8459787] JAMA. 1993 Aug 18;270(7):827-8; author reply 828-9 [8340972] Environ Res. 1994 Apr;65(1):42-55 [8162884] BMJ. 1994 Nov 5;309(6963):1189-97 [7987149] Neurotoxicol Teratol. 1995 May-Jun;17(3):223-5; discussion 249-51 [7542730] Comment In: Environ Health Perspect. 2005 Sep;113(9):A581-2 [16140604] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Initial evaluation of the effects of aerosolized Florida red tide toxins (brevetoxins) in persons with asthma. AN - 67780286; 15866779 AB - Florida red tides annually occur in the Gulf of Mexico, resulting from blooms of the marine dinoflagellate Karenia brevis. K. brevis produces highly potent natural polyether toxins, known as brevetoxins, that activate voltage-sensitive sodium channels. In experimental animals, brevetoxins cause significant bronchoconstriction. A study of persons who visited the beach recreationally found a significant increase in self-reported respiratory symptoms after exposure to aerosolized Florida red tides. Anecdotal reports indicate that persons with underlying respiratory diseases may be particularly susceptible to adverse health effects from these aerosolized toxins. Fifty-nine persons with physician-diagnosed asthma were evaluated for 1 hr before and after going to the beach on days with and without Florida red tide. Study participants were evaluated with a brief symptom questionnaire, nose and throat swabs, and spirometry approved by the National Institute for Occupational Safety and Health. Environmental monitoring, water and air sampling (i.e., K. brevis, brevetoxins, and particulate size distribution), and personal monitoring (for toxins) were performed. Brevetoxin concentrations were measured by liquid chromatography mass spectrometry, high-performance liquid chromatography, and a newly developed brevetoxin enzyme-linked immunosorbent assay. Participants were significantly more likely to report respiratory symptoms after Florida red tide exposure. Participants demonstrated small but statistically significant decreases in forced expiratory volume in 1 sec, forced expiratory flow between 25 and 75%, and peak expiratory flow after exposure, particularly those regularly using asthma medications. Similar evaluation during nonexposure periods did not significantly differ. This is the first study to show objectively measurable adverse health effects from exposure to aerosolized Florida red tide toxins in persons with asthma. Future studies will examine the possible chronic effects of these toxins among persons with asthma and other chronic respiratory impairment. JF - Environmental health perspectives AU - Fleming, Lora E AU - Kirkpatrick, Barbara AU - Backer, Lorraine C AU - Bean, Judy A AU - Wanner, Adam AU - Dalpra, Dana AU - Tamer, Robert AU - Zaias, Julia AU - Cheng, Yung Sung AU - Pierce, Richard AU - Naar, Jerome AU - Abraham, William AU - Clark, Richard AU - Zhou, Yue AU - Henry, Michael S AU - Johnson, David AU - Van De Bogart, Gayl AU - Bossart, Gregory D AU - Harrington, Mark AU - Baden, Daniel G AD - National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Sciences Center, University of Miami Rosenstiel School of Marine and Atmospheric Sciences, Miami, FL 33136, USA. lfleming@med.miami.edu Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 650 EP - 657 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Aerosols KW - 0 KW - Marine Toxins KW - Oxocins KW - brevetoxin KW - 98225-48-0 KW - Index Medicus KW - Respiratory Function Tests KW - Animals KW - Mass Spectrometry KW - Humans KW - Aged KW - Child KW - Florida KW - Chromatography, High Pressure Liquid KW - Recreation KW - Adult KW - Middle Aged KW - Adolescent KW - Female KW - Male KW - Asthma -- etiology KW - Oxocins -- adverse effects KW - Inhalation Exposure KW - Dinoflagellida -- pathogenicity KW - Marine Toxins -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67780286?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+health+perspectives&rft.atitle=Initial+evaluation+of+the+effects+of+aerosolized+Florida+red+tide+toxins+%28brevetoxins%29+in+persons+with+asthma.&rft.au=Fleming%2C+Lora+E%3BKirkpatrick%2C+Barbara%3BBacker%2C+Lorraine+C%3BBean%2C+Judy+A%3BWanner%2C+Adam%3BDalpra%2C+Dana%3BTamer%2C+Robert%3BZaias%2C+Julia%3BCheng%2C+Yung+Sung%3BPierce%2C+Richard%3BNaar%2C+Jerome%3BAbraham%2C+William%3BClark%2C+Richard%3BZhou%2C+Yue%3BHenry%2C+Michael+S%3BJohnson%2C+David%3BVan+De+Bogart%2C+Gayl%3BBossart%2C+Gregory+D%3BHarrington%2C+Mark%3BBaden%2C+Daniel+G&rft.aulast=Fleming&rft.aufirst=Lora&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=650&rft.isbn=&rft.btitle=&rft.title=Environmental+health+perspectives&rft.issn=00916765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-16 N1 - Date created - 2005-05-03 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Am J Respir Crit Care Med. 1999 Dec;160(6):2006-11 [10588621] Environ Health Perspect. 2005 May;113(5):644-9 [15866778] Chem Biol. 2000 Jun;7(6):385-93 [10873835] Int J Tuberc Lung Dis. 2000 Jul;4(7):633-8 [10907766] Environ Toxicol Chem. 2001 Jan;20(1):107-14 [11351396] Environ Health Perspect. 2002 Feb;110(2):179-85 [11836147] Respiration. 2002;69(1):38-45 [11844961] Environ Res. 2002 May;89(1):29-37 [12051782] Occup Environ Med. 2002 Sep;59(9):629-33 [12205238] Bull Environ Contam Toxicol. 2003 Jan;70(1):161-5 [12478439] Am J Ind Med. 2003 Feb;43(2):196-203 [12541275] Ann Allergy Asthma Immunol. 2003 May;90(5 Suppl 2):47-51 [12772952] Cell Mol Neurobiol. 2004 Aug;24(4):553-63 [15233378] JFMA. 1973 Nov;60(11):27-9 [4755462] J Allergy Clin Immunol. 1982 May;69(5):418-28 [7200498] Int Rev Cytol. 1983;82:99-150 [6352551] J Toxicol Environ Health. 1985;15(2):197-214 [3892021] Toxicon. 1989;27(6):647-54 [2546295] Am J Public Health. 1991 Apr;81(4):471-4 [2003627] Am Rev Respir Dis. 1991 Nov;144(5):1202-18 [1952453] Am J Respir Crit Care Med. 1995 Sep;152(3):1107-36 [7663792] Am J Ind Med. 1997 Jun;31(6):671-7 [9131220] Toxicol Pathol. 1998 Mar-Apr;26(2):276-82 [9547868] Am J Respir Crit Care Med. 1999 Jan;159(1):179-87 [9872837] J Toxicol Environ Health A. 1999 Jul 9;57(5):345-55 [10405188] Environ Health Perspect. 2005 May;113(5):621-5 [15866774] Environ Health Perspect. 2005 May;113(5):626-31 [15866775] Environ Health Perspect. 2005 May;113(5):632-7 [15866776] Environ Health Perspect. 2005 May;113(5):638-43 [15866777] Toxicon. 2000 Jul;38(7):981-93 [10728835] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serpiginous choroiditis. AN - 67778535; 15850812 AB - Serpiginous choroiditis is a rare, usually bilateral, chronic, progressive, recurrent inflammation of the choroid, retinal pigment epithelium, and choriocapillaris of unknown etiology. Based on clinical presentation, it can be classified into 1) peripapillary, 2) macular, and 3) ampiginous types. The clinical course, regardless of the presentation, is progressive with multiple recurrences leading to potentially significant visual loss. Visual outcome is directly related to the involvement of the para-fovea and fovea by the lesions or secondary choroidal neovascularization. The histological findings of the lesions are atrophy of the choriocapillaris, retinal pigment epithelium and photoreceptor cells, and moderate diffuse lymphocytic infiltrates throughout the choroid. Multiple etiologies including autoimmunity, infection, vasculopathy, and degeneration were proposed but none is well supported by clinical and laboratory evidence. Fluorescein and indocyanine green angiography have been useful in the assessment of the extent and the activity of lesions. Due to the insidious and progressive clinical course, an assessment of treatment outcomes needs long term follow-up. Currently, treatment with immunosuppressive and alkylating agents have shown possible efficacy in small case series. Larger clinical studies and interventional trials are required to further our understanding of the pathogenesis, etiology, and for the evaluation of treatment strategies. JF - Survey of ophthalmology AU - Lim, Wee-Kiak AU - Buggage, Ronald R AU - Nussenblatt, Robert B AD - National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA; and Singapore National Eye Centre, Singapore. PY - 2005 SP - 231 EP - 244 VL - 50 IS - 3 SN - 0039-6257, 0039-6257 KW - Antimetabolites, Antineoplastic KW - 0 KW - Coloring Agents KW - Immunosuppressive Agents KW - Indocyanine Green KW - IX6J1063HV KW - Index Medicus KW - Photoreceptor Cells, Vertebrate -- pathology KW - Diagnosis, Differential KW - Humans KW - Fluorescein Angiography KW - Atrophy KW - Pigment Epithelium of Eye -- pathology KW - Immunosuppressive Agents -- therapeutic use KW - Antimetabolites, Antineoplastic -- therapeutic use KW - Choroiditis -- diagnosis KW - Choroiditis -- epidemiology KW - Choroiditis -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67778535?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Survey+of+ophthalmology&rft.atitle=Serpiginous+choroiditis.&rft.au=Lim%2C+Wee-Kiak%3BBuggage%2C+Ronald+R%3BNussenblatt%2C+Robert+B&rft.aulast=Lim&rft.aufirst=Wee-Kiak&rft.date=2005-05-01&rft.volume=50&rft.issue=3&rft.spage=231&rft.isbn=&rft.btitle=&rft.title=Survey+of+ophthalmology&rft.issn=00396257&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-29 N1 - Date created - 2005-04-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A Phase I clinical trial of ixabepilone (BMS-247550), an epothilone B analog, administered intravenously on a daily schedule for 3 days. AN - 67770263; 15800893 AB - The epothilones are a novel class of microtubule-stabilizing agents. Ixabepilone (BMS-247550; NSC 710428) is a semisynthetic analog of the natural product epothilone B. The authors conducted a Phase I study by administering ixabepilone to patients as a 1-hour intravenous infusion daily for 3 consecutive days every 21 days. Twenty-six patients were enrolled and received ixabepilone at a starting dose of 8 or 10 mg/m(2) per day for 3 consecutive days. One hundred and nineteen cycles were administered to 26 patients. The maximum-tolerated dose was 8 mg/m(2) per day of ixabepilone administered as a 1-hour intravenous infusion daily for 3 consecutive days every 21 days. The dose-limiting toxicity (DLT) was neutropenia. Other nonhematologic Grade 3 toxicities included fatigue (3 cycles), hyponatremia (1 cycle), anorexia (1 cycle), ileus (1 cycle), stomatitis (1 cycle), and emesis (1 cycle). Prolonged disease stabilization was observed in patients with mesothelioma, ovarian carcinoma, and renal cell carcinoma. The recommended Phase II dose of ixabepilone on the daily schedule for 3 days was 8-10 mg/m(2) per day. Neutropenia was the DLT. Peripheral neuropathy was mild, even after multiple cycles of therapy, and was not dose limiting. Published 2005 by the American Cancer Society. JF - Cancer AU - Zhuang, Sen H AU - Agrawal, Manish AU - Edgerly, Maureen AU - Bakke, Susan AU - Kotz, Herb AU - Thambi, Paul AU - Rutt, Ann AU - Balis, Frank M AU - Bates, Susan AU - Fojo, Tito AD - Cancer Therapeutics Branch, Center of Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 1932 EP - 1938 VL - 103 IS - 9 SN - 0008-543X, 0008-543X KW - Epothilones KW - 0 KW - ixabepilone KW - K27005NP0A KW - Abridged Index Medicus KW - Index Medicus KW - Drug Administration Schedule KW - Infusions, Intravenous KW - Aged, 80 and over KW - Humans KW - Adult KW - Aged KW - Middle Aged KW - Maximum Tolerated Dose KW - Male KW - Female KW - Neoplasms -- drug therapy KW - Epothilones -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67770263?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer&rft.atitle=A+Phase+I+clinical+trial+of+ixabepilone+%28BMS-247550%29%2C+an+epothilone+B+analog%2C+administered+intravenously+on+a+daily+schedule+for+3+days.&rft.au=Zhuang%2C+Sen+H%3BAgrawal%2C+Manish%3BEdgerly%2C+Maureen%3BBakke%2C+Susan%3BKotz%2C+Herb%3BThambi%2C+Paul%3BRutt%2C+Ann%3BBalis%2C+Frank+M%3BBates%2C+Susan%3BFojo%2C+Tito&rft.aulast=Zhuang&rft.aufirst=Sen&rft.date=2005-05-01&rft.volume=103&rft.issue=9&rft.spage=1932&rft.isbn=&rft.btitle=&rft.title=Cancer&rft.issn=0008543X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-07 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An essential role for phospholipase D in the activation of protein kinase C and degranulation in mast cells. AN - 67768072; 15843515 AB - Activation of phospholipase D (PLD) and protein kinase C (PKC) as well as calcium mobilization are essential signals for degranulation of mast cells. However, the exact role of PLD in degranulation remains undefined. In this study we have tested the hypothesis that the PLD product, phosphatidic acid, and diacylglycerides generated therefrom might promote activation of PKC. Studies were conducted in two rodent mast cell lines that were stimulated with Ag via FcepsilonRI and a pharmacologic agent, thapsigargin. Diversion of production of phosphatidic acid to phosphatidylbutanol (the transphosphatidylation reaction) by addition of l-butanol suppressed both the translocation of diacylglyceride-dependent isoforms of PKC to the membrane and degranulation. Tertiary-butanol, which is not a substrate for the transphosphatidylation, had a minimal effect on PKC translocation and degranulation, and 1-butanol itself had no effect on PKC translocation when PKC was stimulated directly with phorbol ester, 12-O-tetradecanoylphorbol-13-acetate. Also, in cells transfected with small inhibitory RNAs directed against PLD1 and PLD2, activation of PLD, generation of diacylglycerides, translocation of PKC, and degranulation were all suppressed. Phorbol ester, which did not stimulate degranulation by itself, restored degranulation when used in combination with thapsigargin whether PLD function was disrupted with 1-butanol or the small inhibitory RNAs. However, degranulation was not restored when cells were costimulated with Ag and phorbol ester. These results suggested that the production of phosphatidic acid by PLD facilitates activation of PKC and, in turn, degranulation, although additional PLD-dependent processes appear to be critical for Ag-mediated degranulation. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Peng, Ze AU - Beaven, Michael A AD - Laboratory of Molecular Immunology, National, Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5201 EP - 5208 VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - Diglycerides KW - 0 KW - Enzyme Inhibitors KW - Isoenzymes KW - Phosphatidic Acids KW - RNA, Small Interfering KW - Thapsigargin KW - 67526-95-8 KW - 1-Butanol KW - 8PJ61P6TS3 KW - Protein Kinase C KW - EC 2.7.11.13 KW - Phospholipase D KW - EC 3.1.4.4 KW - Tetradecanoylphorbol Acetate KW - NI40JAQ945 KW - Abridged Index Medicus KW - Index Medicus KW - 1-Butanol -- pharmacology KW - Phosphatidic Acids -- biosynthesis KW - Animals KW - Phosphatidic Acids -- antagonists & inhibitors KW - Protein Transport -- drug effects KW - Diglycerides -- biosynthesis KW - Mice KW - 1-Butanol -- antagonists & inhibitors KW - Cell Line, Tumor KW - Isoenzymes -- metabolism KW - Thapsigargin -- pharmacology KW - Rats KW - Isoenzymes -- antagonists & inhibitors KW - Phosphorylation KW - Mice, Inbred C57BL KW - Enzyme Activation -- drug effects KW - Tetradecanoylphorbol Acetate -- pharmacology KW - RNA, Small Interfering -- pharmacology KW - Enzyme Inhibitors -- pharmacology KW - Cell Line, Transformed KW - Drug Synergism KW - Enzyme Activation -- immunology KW - Diglycerides -- antagonists & inhibitors KW - Protein Transport -- immunology KW - Protein Kinase C -- metabolism KW - Protein Kinase C -- antagonists & inhibitors KW - Mast Cells -- metabolism KW - Phospholipase D -- antagonists & inhibitors KW - Mast Cells -- enzymology KW - Phospholipase D -- physiology KW - Mast Cells -- drug effects KW - Cell Degranulation -- immunology KW - Cell Degranulation -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67768072?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=An+essential+role+for+phospholipase+D+in+the+activation+of+protein+kinase+C+and+degranulation+in+mast+cells.&rft.au=Peng%2C+Ze%3BBeaven%2C+Michael+A&rft.aulast=Peng&rft.aufirst=Ze&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5201&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Comparison of asbestos exposure assessments by next-of-kin respondents, by an occupational hygienist, and by a job-exposure matrix from the National Occupational Hazard Survey. AN - 67759019; 15828074 AB - Assessments of occupational exposures in case-control studies of rapidly fatal illnesses often rely on data from next-of-kin respondents, which may be inaccurate. Three methods for assessing exposure to asbestos from case-control data on mesothelioma, including next-of-kin assessment, expert assessment, and use of a generic job-exposure matrix (JEM). Interview data [Spirtas et al. (1994): Occup Environ Med 51:804-811] were reviewed to determine exposure status by an occupational hygienist (C.R.) who was unaware of disease status. Exposure odds ratios were calculated using standard methods, and measures of agreement included the kappa statistic and conditional and marginal odds ratios. Expert assessment detected higher proportions of exposed subjects than the next-of-kin respondents or JEM methods. The disease-exposure odds ratios were highest for respondents, perhaps because of recall bias, and lowest for the JEM method. The agreement was highest between the respondent and expert assessments. A combination of respondent's assessment and JEM assessment led to the best prediction of the expert's assessment. Results for spouse respondents were similar to those for other "next-of-kin" respondents. Expert assessments were the most plausible, but the data indicate that disease associations could also be detected with the other exposure assessment methods. Using some combination of the proxy respondent's assessment and the JEM assessment, one can predict the expert's assessment. A strategy that relied on the respondent's assessment when it was positive and otherwise obtained an expert assessment could reduce costs with little error, compared to expert assessment on all subjects. Published 2005 Wiley-Liss, Inc. JF - American journal of industrial medicine AU - Nam, Jun-mo AU - Rice, Carol AU - Gail, Mitchell H AD - Biostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health & Human Services, Bethesda, Maryland, USA. namj@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 443 EP - 450 VL - 47 IS - 5 SN - 0271-3586, 0271-3586 KW - Asbestos KW - 1332-21-4 KW - Index Medicus KW - Los Angeles -- epidemiology KW - Reproducibility of Results KW - Humans KW - Aged KW - Mental Recall KW - Risk Assessment -- methods KW - New York -- epidemiology KW - Registries KW - Proxy KW - Aged, 80 and over KW - Adult KW - Death Certificates KW - Health Surveys KW - Case-Control Studies KW - Middle Aged KW - Bias (Epidemiology) KW - Occupational Medicine KW - Occupational Exposure -- statistics & numerical data KW - Mesothelioma -- epidemiology KW - Mesothelioma -- etiology KW - Mesothelioma -- mortality KW - Occupational Exposure -- adverse effects KW - Family KW - Asbestos -- toxicity KW - Occupational Exposure -- analysis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67759019?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3A&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology&rft.atitle=Neural+basis+of+mindfulness+interventions+that+moderate+the+impact+of+stress+on+the+brain&rft.au=Paulus%2C+Martin+P.&rft.aulast=Paulus&rft.aufirst=Martin&rft.date=2016-01-01&rft.volume=41&rft.issue=1&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/10.1038%2Fnpp.2015.239 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-19 N1 - Date created - 2005-04-21 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Occupational medicine: at a turning point or an expansion. AN - 67755939; 15837845 JF - Occupational and environmental medicine AU - Blair, A AD - blaira@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 VL - 62 IS - 5 KW - Index Medicus KW - Occupational Health KW - Culture KW - Humans KW - Neoplasms -- epidemiology KW - Occupational Exposure -- adverse effects KW - Risk Assessment -- methods KW - Neoplasms -- etiology KW - Occupational Diseases -- etiology KW - Occupational Diseases -- psychology KW - Occupational Diseases -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67755939?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Occupational+and+environmental+medicine&rft.atitle=Occupational+medicine%3A+at+a+turning+point+or+an+expansion.&rft.au=Blair%2C+A&rft.aulast=Blair&rft.aufirst=A&rft.date=2005-05-01&rft.volume=62&rft.issue=5&rft.spage=285%3B+discussion+287&rft.isbn=&rft.btitle=&rft.title=Occupational+and+environmental+medicine&rft.issn=1470-7926&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment On: Occup Environ Med. 2005 May;62(5):281-3 [15837843] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ephedrine plus caffeine causes age-dependent cardiovascular responses in Fischer 344 rats. AN - 67754760; 15653753 AB - Human consumption of ephedrine and caffeine in dietary supplements has been associated with a number of adverse effects including changes in the ECG, myocardial infarction, hyperthermia, and, in rare instances, death. The purpose of this study was to investigate the potential mechanisms associated with the cardiotoxicity of combined ephedrine and caffeine ingestion. Seven- and fourteen-week-old Fischer 344 rats treated with ephedrine in combination with caffeine exhibited increases in heart rate (HR), temperature, and corrected QT interval. Of the 14-wk-old rats treated with 25 mg/kg ephedrine plus 30 mg/kg caffeine, 57% died within 3-5 h of treatment, whereas none of the similarly treated 7-wk-old rats nor any of the rats treated with vehicle died. One hour after treatment with this dose of ephedrine plus caffeine, 14-wk-old rats exhibited a larger increase in HR (as % increase over baseline) than 7-wk-old rats. Furthermore, the 14-wk-old rats that died had a higher HR and temperature than the 14-wk-old rats that lived. Histopathological studies suggested interstitial hemorrhage and myofiber necrosis in the 14-wk-old rats treated with the highest concentration of ephedrine and caffeine. This study showed enhanced susceptibility to ephedrine plus caffeine in 14-wk-old rats compared with 7-wk-old rats. The greater mortality in the 14-wk-old rats was associated with increases in body temperature, HR, and myocardial necrosis. JF - American journal of physiology. Heart and circulatory physiology AU - Howden, Reuben AU - Hanlon, Paul R AU - Petranka, John G AU - Kleeberger, Steven AU - Bucher, John AU - Dunnick, June AU - Nyska, Abraham AU - Murphy, Elizabeth AD - Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - H2219 EP - H2224 VL - 288 IS - 5 SN - 0363-6135, 0363-6135 KW - Central Nervous System Stimulants KW - 0 KW - Caffeine KW - 3G6A5W338E KW - Ephedrine KW - GN83C131XS KW - Index Medicus KW - Rats KW - Animals KW - Rats, Inbred F344 KW - Age Factors KW - Body Temperature -- drug effects KW - In Vitro Techniques KW - Papillary Muscles -- drug effects KW - Drug Synergism KW - Papillary Muscles -- pathology KW - Male KW - Electrocardiography -- drug effects KW - Fever -- chemically induced KW - Heart Rate -- drug effects KW - Fever -- pathology KW - Caffeine -- toxicity KW - Central Nervous System Stimulants -- toxicity KW - Fever -- mortality KW - Ephedrine -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67754760?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+physiology.+Heart+and+circulatory+physiology&rft.atitle=Ephedrine+plus+caffeine+causes+age-dependent+cardiovascular+responses+in+Fischer+344+rats.&rft.au=Howden%2C+Reuben%3BHanlon%2C+Paul+R%3BPetranka%2C+John+G%3BKleeberger%2C+Steven%3BBucher%2C+John%3BDunnick%2C+June%3BNyska%2C+Abraham%3BMurphy%2C+Elizabeth&rft.aulast=Howden&rft.aufirst=Reuben&rft.date=2005-05-01&rft.volume=288&rft.issue=5&rft.spage=H2219&rft.isbn=&rft.btitle=&rft.title=American+journal+of+physiology.+Heart+and+circulatory+physiology&rft.issn=03636135&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-31 N1 - Date created - 2005-04-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Advancing cancer biotherapy with proteomics. AN - 67754233; 15838374 AB - Proteomics is becoming increasingly important for cancer biotherapy. The development of high-throughput platforms now allows the analysis of multiple proteins from small quantities of material. While these techniques are being used to discover new biomarkers, they are particularly important for assessing complex biologic processes such as immunotherapy for cancer. Recent advances in this field are reviewed, as well as the use of proteomics to assess the effectiveness and toxicities of high-dose IL-2 cancer therapy. Proteomics is becoming useful in assessing cancer biotherapies and in unraveling their mechanisms of action. High-throughput proteomic technologies have now advanced to a stage where they have the potential to become effective discovery tools for biomarkers/predictors of disease, disease recurrence, and response to therapy. JF - Journal of immunotherapy (Hagerstown, Md. : 1997) AU - Stroncek, David F AU - Burns, Christine AU - Martin, Brian M AU - Rossi, Leonardo AU - Marincola, Francesco M AU - Panelli, Monica C AD - Immunogenetics Section, Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1148, USA. dstroncek@cc.nih.gov PY - 2005 SP - 183 EP - 192 VL - 28 IS - 3 SN - 1524-9557, 1524-9557 KW - Interleukin-2 KW - 0 KW - Index Medicus KW - Humans KW - Proteomics -- methods KW - Interleukin-2 -- therapeutic use KW - Neoplasms -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67754233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunotherapy+%28Hagerstown%2C+Md.+%3A+1997%29&rft.atitle=Advancing+cancer+biotherapy+with+proteomics.&rft.au=Stroncek%2C+David+F%3BBurns%2C+Christine%3BMartin%2C+Brian+M%3BRossi%2C+Leonardo%3BMarincola%2C+Francesco+M%3BPanelli%2C+Monica+C&rft.aulast=Stroncek&rft.aufirst=David&rft.date=2005-05-01&rft.volume=28&rft.issue=3&rft.spage=183&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunotherapy+%28Hagerstown%2C+Md.+%3A+1997%29&rft.issn=15249557&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-03 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - RhoA signaling is required for respiratory syncytial virus-induced syncytium formation and filamentous virion morphology. AN - 67744070; 15827147 AB - Respiratory syncytial virus (RSV) is an important human pathogen that can cause severe and life-threatening respiratory infections in infants, the elderly, and immunocompromised adults. RSV infection of HEp-2 cells induces the activation of RhoA, a small GTPase. We therefore asked whether RhoA signaling is important for RSV replication or syncytium formation. The treatment of HEp-2 cells with Clostridium botulinum C3, an enzyme that ADP-ribosylates and specifically inactivates RhoA, inhibited RSV-induced syncytium formation and cell-to-cell fusion, although similar levels of PFU were released into the medium and viral protein expression levels were equivalent. Treatment with another inhibitor of RhoA signaling, the Rho kinase inhibitor Y-27632, yielded similar results. Scanning electron microscopy of C3-treated infected cells showed reduced numbers of single blunted filaments, in contrast to the large clumps of long filaments in untreated infected cells. These data suggest that RhoA signaling is associated with filamentous virus morphology, cell-to-cell fusion, and syncytium formation but is dispensable for the efficient infection and production of infectious virus in vitro. Next, we developed a semiquantitative method to measure spherical and filamentous virus particles by using sucrose gradient velocity sedimentation. Fluorescence and transmission electron microscopy confirmed the separation of spherical and filamentous forms of infectious virus into two identifiable peaks. The C3 treatment of RSV-infected cells resulted in a shift to relatively more spherical virions than those from untreated cells. These data suggest that viral filamentous protuberances characteristic of RSV infection are associated with RhoA signaling, are important for filamentous virion morphology, and may play a role in initiating cell-to-cell fusion. JF - Journal of virology AU - Gower, Tara L AU - Pastey, Manoj K AU - Peeples, Mark E AU - Collins, Peter L AU - McCurdy, Lewis H AU - Hart, Timothy K AU - Guth, Alex AU - Johnson, Teresa R AU - Graham, Barney S AD - Vaccine Research Center, Building 40, Room 2502, NIAID, NIH, 40 Convent Dr., MSC 3017, Bethesda, MD 20892-3017, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 5326 EP - 5336 VL - 79 IS - 9 SN - 0022-538X, 0022-538X KW - Amides KW - 0 KW - Pyridines KW - Y 27632 KW - 138381-45-0 KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - exoenzyme C3, Clostridium botulinum KW - Botulinum Toxins KW - EC 3.4.24.69 KW - rhoA GTP-Binding Protein KW - EC 3.6.5.2 KW - Index Medicus KW - Virus Replication KW - Giant Cells -- ultrastructure KW - Amides -- pharmacology KW - Humans KW - Botulinum Toxins -- pharmacology KW - ADP Ribose Transferases -- pharmacology KW - Pyridines -- pharmacology KW - Cell Line KW - Respiratory Syncytial Viruses -- drug effects KW - rhoA GTP-Binding Protein -- metabolism KW - Respiratory Syncytial Viruses -- physiology KW - Respiratory Syncytial Viruses -- ultrastructure KW - Signal Transduction KW - rhoA GTP-Binding Protein -- antagonists & inhibitors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67744070?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+virology&rft.atitle=RhoA+signaling+is+required+for+respiratory+syncytial+virus-induced+syncytium+formation+and+filamentous+virion+morphology.&rft.au=Gower%2C+Tara+L%3BPastey%2C+Manoj+K%3BPeeples%2C+Mark+E%3BCollins%2C+Peter+L%3BMcCurdy%2C+Lewis+H%3BHart%2C+Timothy+K%3BGuth%2C+Alex%3BJohnson%2C+Teresa+R%3BGraham%2C+Barney+S&rft.aulast=Gower&rft.aufirst=Tara&rft.date=2005-05-01&rft.volume=79&rft.issue=9&rft.spage=5326&rft.isbn=&rft.btitle=&rft.title=Journal+of+virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-03 N1 - Date created - 2005-04-13 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Biol Chem. 1999 Jul 23;274(30):21430-6 [10409706] Virology. 1996 Mar 1;217(1):76-87 [8599238] J Virol. 2000 Jan;74(1):228-36 [10590110] J Virol. 2000 Apr;74(7):3264-72 [10708443] J Gen Virol. 2000 May;81(Pt 5):1305-12 [10769073] J Virol. 2000 May;74(10):4634-44 [10775599] Blood. 2000 May 15;95(10):3044-51 [10807767] Virology. 2000 Jun 5;271(2):264-75 [10860881] Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14172-7 [11106388] Virology. 2001 May 10;283(2):188-96 [11336544] J Virol. 2002 Apr;76(8):3952-64 [11907235] J Gen Virol. 2002 Aug;83(Pt 8):1841-50 [12124448] J Virol. 2003 Feb;77(3):1747-56 [12525608] J Virol. 2003 Mar;77(6):3785-98 [12610153] Arch Gesamte Virusforsch. 1973;41(3):248-58 [4353798] J Virol. 1973 Nov;12(5):1173-80 [4128827] J Gen Virol. 1979 Aug;44(2):479-91 [118236] Arch Virol. 1980;63(3-4):275-84 [6766713] J Med Virol. 1979;4(3):201-11 [94087] J Cell Biol. 1987 Oct;105(4):1473-8 [3312229] AIDS Res Hum Retroviruses. 1987 Fall;3(3):245-52 [3501726] J Cell Biol. 1988 Nov;107(5):1689-95 [3182934] J Med Virol. 1988 Oct;26(2):153-62 [3183639] J Biol Chem. 1989 May 25;264(15):8602-5 [2498316] Membr Biochem. 1987-1988;7(4):231-47 [2855807] J Gen Virol. 1990 Dec;71 ( Pt 12):3075-80 [2177097] Cell. 1991 Mar 8;64(5):915-25 [1900457] J Gen Virol. 1991 Dec;72 ( Pt 12):3095-101 [1765771] Cell. 1992 Aug 7;70(3):389-99 [1643657] Nature. 1993 Mar 25;362(6418):318-24 [8455717] Cell. 1993 Nov 5;75(3):409-18 [8221884] Trends Microbiol. 1993 Nov;1(8):287-8 [8162412] Cell. 1996 Mar 22;84(6):941-51 [8601317] J Virol. 1996 Jul;70(7):4502-8 [8676475] J Biochem. 1996 Aug;120(2):215-28 [8889802] Mol Biol Cell. 1996 Nov;7(11):1825-34 [8930903] Nihon Yakurigaku Zasshi. 1997 Jan;109(1):13-7 [9067995] Trends Microbiol. 1997 Apr;5(4):142-8 [9141188] J Gen Virol. 1997 Aug;78 ( Pt 8):1885-9 [9266983] Nature. 1997 Oct 30;389(6654):990-4 [9353125] Virology. 1998 Jan 5;240(1):127-37 [9448697] Mol Biol Cell. 1998 Feb;9(2):403-19 [9450964] Proc Natl Acad Sci U S A. 1998 May 12;95(10):5746-51 [9576955] Science. 1998 Jun 26;280(5372):2074-5 [9669963] Virology. 1998 Aug 15;248(1):20-34 [9705252] J Virol. 1998 Dec;72(12):9561-6 [9811689] J Biol Chem. 1998 Dec 25;273(52):34663-6 [9856983] Virology. 1998 Dec 5;252(1):137-48 [9875324] Virology. 1994 May 1;200(2):801-5 [8178462] J Cell Biol. 1994 Jun;125(6):1371-84 [8207064] Cell. 1994 Sep 23;78(6):937-48 [7923363] J Cell Sci. 1995 Sep;108 ( Pt 9):3127-35 [8537452] Exp Cell Res. 1999 Jan 10;246(1):83-90 [9882517] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Lorazepam reinstates punishment-suppressed remifentanil self-administration in rats. AN - 67730403; 15821953 AB - We recently described a reinstatement procedure that models relapse to drug abuse in cases where abstinence results from aversive consequences of drug use. The potential value of this punishment-based model of relapse depends on its sensitivity to relapse-inducing events that are ineffective in the widely used extinction-based model. It is known that certain drugs can have anti-punishment effects, but these drugs have not been tested in the punishment-based reinstatement procedure. Therefore, the effects of the benzodiazepine, lorazepam, were examined using punishment-based and extinction-based reinstatement procedures. Rats self-administered the opioid, remifentanil (4 microg/kg per infusion). Two punishment groups were trained with response-contingent footshock that suppressed baseline rates of responding to zero. In an extinction group, remifentanil delivery was discontinued, and baseline responding stabilized at a low rate (mean=0.06 responses/min). Lorazepam (0.08-10 mg/kg, IP) was given during test sessions with the shock contingency discontinued for both punishment groups. Remifentanil delivery was maintained during testing in one punishment group but not the other. Lorazepam reinstated self-administration responding in both punishment groups but not in the extinction group. Priming injections of heroin reinstated responding in both the punishment and extinction groups, but combining heroin and lorazepam did not enhance reinstatement. This is the first demonstration that a trigger for relapse may have different effects depending on whether aversive conditioning contributed to the achievement of abstinence. It may be important to consider potential anti-punishment effects of both abused drugs and therapeutic agents in the treatment of individuals with a history of drug abuse. JF - Psychopharmacology AU - Panlilio, Leigh V AU - Thorndike, Eric B AU - Schindler, Charles W AD - Preclinical Pharmacology Section, Behavioral Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, NIH, DHHS, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA. lpanlili@intra.nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 374 EP - 382 VL - 179 IS - 2 SN - 0033-3158, 0033-3158 KW - Analgesics, Opioid KW - 0 KW - Anti-Anxiety Agents KW - Piperidines KW - Heroin KW - 70D95007SX KW - Lorazepam KW - O26FZP769L KW - remifentanil KW - P10582JYYK KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Heroin -- pharmacology KW - Self Administration KW - Dose-Response Relationship, Drug KW - Extinction, Psychological -- drug effects KW - Heroin Dependence -- psychology KW - Electroshock KW - Male KW - Piperidines -- pharmacology KW - Conditioning, Operant -- drug effects KW - Anti-Anxiety Agents -- pharmacology KW - Analgesics, Opioid -- pharmacology KW - Punishment KW - Substance-Related Disorders -- psychology KW - Lorazepam -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67730403?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Lorazepam+reinstates+punishment-suppressed+remifentanil+self-administration+in+rats.&rft.au=Panlilio%2C+Leigh+V%3BThorndike%2C+Eric+B%3BSchindler%2C+Charles+W&rft.aulast=Panlilio&rft.aufirst=Leigh&rft.date=2005-05-01&rft.volume=179&rft.issue=2&rft.spage=374&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Calorie restriction increases cigarette use in adult smokers. AN - 67723943; 15565433 AB - Cigarette smokers weigh less than nonsmokers, and smokers often gain weight when they quit. This is a major barrier to smoking cessation, especially among women. However, strict dieting is not recommended during smoking cessation out of concern that it might promote relapse. This concern derives, in part, from the observation that calorie restriction increases self-administration of drugs of abuse in animals. This relationship has never been experimentally demonstrated in humans. To evaluate whether calorie restriction increases cigarette smoking in humans. Seventeen (nine males, eight females) healthy, normal-weight smokers not attempting to quit were cycled in partially counterbalanced order, double-blind, through four diets-normal calorie (2,000-2,800 kcal/day), low calorie (700 kcal/day deficit), low-carbohydrate (CHO)/normal-calorie, and low-CHO/low-calorie-for 6 days per diet in an inpatient research ward. Smoking was assessed by cigarette counts, breath carbon monoxide (CO) levels, and cigarette craving. Compared with the normal-calorie diet, while on the low-calorie diet, subjects smoked 8% more cigarettes (P<0.02) and had 11% higher breath CO levels (P<0.01). The low-CHO/normal-calorie diet showed no significant effect on either variable, but there was a 15% increase in breath CO levels (P<0.05) on the low-CHO/low-calorie diet. There were no changes in self-reported cigarette craving or mood. Consistent with animal studies, moderate calorie restriction was associated with a small but statistically significant increase in cigarette smoking, with no independent effect of CHO deprivation. These findings suggest that dieting may increase smoking behavior and could impede smoking-cessation attempts. JF - Psychopharmacology AU - Cheskin, Lawrence J AU - Hess, Judith M AU - Henningfield, Jack AU - Gorelick, David A AD - Department of Health and Human Services, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 430 EP - 436 VL - 179 IS - 2 SN - 0033-3158, 0033-3158 KW - Dietary Carbohydrates KW - 0 KW - Carbon Monoxide KW - 7U1EE4V452 KW - Index Medicus KW - Affect -- drug effects KW - Affect -- physiology KW - Double-Blind Method KW - Sex Characteristics KW - Humans KW - Prospective Studies KW - Adult KW - Tobacco Use Disorder -- psychology KW - Carbon Monoxide -- metabolism KW - Diet KW - Female KW - Male KW - Energy Intake -- physiology KW - Smoking -- metabolism KW - Diet, Reducing KW - Smoking -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67723943?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=Calorie+restriction+increases+cigarette+use+in+adult+smokers.&rft.au=Cheskin%2C+Lawrence+J%3BHess%2C+Judith+M%3BHenningfield%2C+Jack%3BGorelick%2C+David+A&rft.aulast=Cheskin&rft.aufirst=Lawrence&rft.date=2005-05-01&rft.volume=179&rft.issue=2&rft.spage=430&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-10-13 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protection in the aged heart: preventing the heart-break of old age? AN - 67722627; 15820192 AB - The aged heart has a diminished functional and adaptive reserve capacity, an increased susceptibility to incur damage (e.g., as a result of ischemia), and a limited practical ability for repair/regeneration. Thus, there has been considerable interest to harness the heart's endogenous capacity to resist such damage, known as ischemic preconditioning (IPC), as well as other cardioprotective mechanisms. However, the translation of basic research findings into clinical practice has largely been inadequate because there have been few if any successful implementations in terms of viable therapies activating cardioprotection mechanisms to limit infarct size. Here, we provide an overview of the general mechanisms of cardioprotection, changes in the structure and function of the aged heart, and the current knowledge regarding cardioprotection in aged heart. The problems and opportunities for successful bench-to-bedside translation of cardioprotection in the elderly are discussed. JF - Cardiovascular research AU - Juhaszova, Magdalena AU - Rabuel, Christophe AU - Zorov, Dmitry B AU - Lakatta, Edward G AU - Sollott, Steven J AD - Laboratory of Cardiovascular Sciences, Gerontology Research Center, Box 13 Intramural Research Program, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Dr., Baltimore, MD 21224-6825, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 233 EP - 244 VL - 66 IS - 2 SN - 0008-6363, 0008-6363 KW - Cardiotonic Agents KW - 0 KW - Reactive Oxygen Species KW - Glycogen Synthase Kinase 3 KW - EC 2.7.11.26 KW - Index Medicus KW - Models, Animal KW - Cardiotonic Agents -- adverse effects KW - Signal Transduction -- physiology KW - Reactive Oxygen Species -- metabolism KW - Animals KW - Myocardial Reperfusion KW - Humans KW - Cardiotonic Agents -- therapeutic use KW - Aged KW - Mitochondria, Heart -- metabolism KW - Glycogen Synthase Kinase 3 -- antagonists & inhibitors KW - Aging -- physiology KW - Ischemic Preconditioning, Myocardial KW - Myocardial Ischemia -- metabolism KW - Myocardium -- ultrastructure KW - Myocardial Ischemia -- prevention & control KW - Myocardium -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67722627?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cardiovascular+research&rft.atitle=Protection+in+the+aged+heart%3A+preventing+the+heart-break+of+old+age%3F&rft.au=Juhaszova%2C+Magdalena%3BRabuel%2C+Christophe%3BZorov%2C+Dmitry+B%3BLakatta%2C+Edward+G%3BSollott%2C+Steven+J&rft.aulast=Juhaszova&rft.aufirst=Magdalena&rft.date=2005-05-01&rft.volume=66&rft.issue=2&rft.spage=233&rft.isbn=&rft.btitle=&rft.title=Cardiovascular+research&rft.issn=00086363&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-01 N1 - Date created - 2005-04-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Protein-protein interactions and posttranslational modifications in mammalian base excision repair. AN - 67705272; 15808410 AB - Base excision repair (BER) averts the cytotoxic and mutagenic effects of most endogenously produced DNA damage, including lesions that arise spontaneously due to the intrinsic instability of DNA or modifications that are formed from reactions with intracellular chemicals, such as reactive oxygen species and alkylating agents. Defects in the BER process have been associated with cancer susceptibility and neurodegenerative disorders. In its most simplistic form, BER can be fully reconstituted with a minimum of four human proteins and is completed in just five sequential steps: (i) excision of an inappropriate base by a DNA glycosylase (e.g., uracil DNA glycosylase); (ii) incision of the DNA backbone immediately adjacent to the resulting abasic site by apurinic/apyrimidimic endonuclease 1; (iii) removal of the 5'-abasic terminal fragment, and (iv) repair synthesis to fill the gap by DNA polymerase beta; and (v) ligation to seal the remaining nick by DNA ligase 1 or a complex of DNA ligase 3 and X-ray repair cross-complementing 1. However, BER can involve the participation of other proteins as well, such as alternative DNA polymerases or one of several nonessential "auxiliary" factors. In addition, BER operates most efficiently when specific protein-protein coordination occurs. Furthermore, several BER protein activities have been shown to be regulated by posttranslational modification, and some of the physical protein interactions link BER to other DNA transaction pathways. In this review, we summarize the current state of the emerging complexities of mammalian BER, focusing on the growing number of reported protein-protein interactions and posttranslational modifications. JF - Free radical biology & medicine AU - Fan, Jinshui AU - Wilson, David M AD - Laboratory of Molecular Gerontology, GRC, National Institute on Aging, IRP, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA. Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 1121 EP - 1138 VL - 38 IS - 9 SN - 0891-5849, 0891-5849 KW - Proteins KW - 0 KW - Index Medicus KW - Animals KW - Mammals KW - Protein Binding KW - DNA Repair KW - Protein Processing, Post-Translational KW - Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67705272?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Free+radical+biology+%26+medicine&rft.atitle=Protein-protein+interactions+and+posttranslational+modifications+in+mammalian+base+excision+repair.&rft.au=Fan%2C+Jinshui%3BWilson%2C+David+M&rft.aulast=Fan&rft.aufirst=Jinshui&rft.date=2005-05-01&rft.volume=38&rft.issue=9&rft.spage=1121&rft.isbn=&rft.btitle=&rft.title=Free+radical+biology+%26+medicine&rft.issn=08915849&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-09-16 N1 - Date created - 2005-04-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Evidence that HIV-1 Encodes an siRNA and a Suppressor of RNA Silencing AN - 21138615; 6658718 AB - In plants and invertebrate animals, RNA silencing is a form of nucleic acid- based adaptive immunity. By contrast, jawed vertebrates have evolved complex protein-based adaptive immunity. Although short interfering RNAs (siRNAs) have been used as artificial tools to silence viral infection in human cells, it remains unknown whether mammalian viruses naturally elicit such immunity in vertebral cells. Here, we report the evidence that HIV-1 encodes viral siRNA precursors in its genome and that natural HIV-1 infection provokes nucleic acid- based immunity in human cells. To combat this cellular defense, HIV-1 has evolved in its Tat protein a suppressor of RNA silencing (SRS) function. Tat abrogates the cell's RNA-silencing defense by subverting the ability of Dicer to process precursor double-stranded RNAs into siRNAs. JF - Immunity AU - Bennasser, Yamina AU - Le, Shu-Yun AU - Benkirane, Monsef AU - Jeang, Kuan-Teh AD - Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, kj7e@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 607 EP - 619 PB - Cell Press, 1100 Massachusetts Avenue Cambridge MA 02138 USA, [mailto:subs@cell.com], [URL:http://www.cellpress.com] VL - 22 IS - 5 SN - 1074-7613, 1074-7613 KW - HIV-1 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Immunology Abstracts; Virology & AIDS Abstracts KW - Genomes KW - siRNA KW - Tat protein KW - Double-stranded RNA KW - Human immunodeficiency virus 1 KW - RNA-mediated interference KW - Immunity KW - Infection KW - Vertebrae KW - V 22002:AIDS: Molecular and in vitro aspects KW - W 30940:Products KW - N 14830:RNA KW - F 06104:Virus UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21138615?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Immunity&rft.atitle=Evidence+that+HIV-1+Encodes+an+siRNA+and+a+Suppressor+of+RNA+Silencing&rft.au=Bennasser%2C+Yamina%3BLe%2C+Shu-Yun%3BBenkirane%2C+Monsef%3BJeang%2C+Kuan-Teh&rft.aulast=Bennasser&rft.aufirst=Yamina&rft.date=2005-05-01&rft.volume=22&rft.issue=5&rft.spage=607&rft.isbn=&rft.btitle=&rft.title=Immunity&rft.issn=10747613&rft_id=info:doi/10.1016%2Fj.immuni.2005.03.010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Genomes; siRNA; Tat protein; Double-stranded RNA; RNA-mediated interference; Immunity; Infection; Vertebrae; Human immunodeficiency virus 1 DO - http://dx.doi.org/10.1016/j.immuni.2005.03.010 ER - TY - JOUR T1 - Fold recognition aided by constraints from small angle X-ray scattering data AN - 20857189; 6417236 AB - We performed a systematic exploration of the use of structural information derived from small angle X-ray scattering (SAXS) measurements to improve fold recognition. SAXS data provide the Fourier transform of the histogram of atomic pair distances (pair distribution function) for a given protein and hence can serve as a structural constraint on methods used to determine the native conformational fold of the protein. Here we used it to construct a similarity-based fitness score with which to evaluate candidate structures generated by a threading procedure. In order to combine the SAXS scores with the standard energy scores and other 1D profile-based scores used in threading, we made use both of a linear regression method and of a neural network-based technique to obtain optimal combined fitness scores and applied them to the ranking of candidate structures. Our results show that the use of SAXS data with gapless threading significantly improves the performance of fold recognition. JF - Protein Engineering Design and Selection AU - Zheng, Wenjun AU - Doniach, Sebastian AD - Departments of Physics and Applied Physics and Laboratory for Advanced Materials, Stanford University, CA 94305 and Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 209 EP - 219 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 18 IS - 5 SN - 1741-0126, 1741-0126 KW - Biotechnology and Bioengineering Abstracts KW - Fitness KW - Data processing KW - Protein folding KW - Energy KW - X-ray scattering KW - W 30925:Genetic Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20857189?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Engineering+Design+and+Selection&rft.atitle=Fold+recognition+aided+by+constraints+from+small+angle+X-ray+scattering+data&rft.au=Zheng%2C+Wenjun%3BDoniach%2C+Sebastian&rft.aulast=Zheng&rft.aufirst=Wenjun&rft.date=2005-05-01&rft.volume=18&rft.issue=5&rft.spage=209&rft.isbn=&rft.btitle=&rft.title=Protein+Engineering+Design+and+Selection&rft.issn=17410126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-09-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Data processing; Protein folding; Fitness; X-ray scattering; Energy ER - TY - JOUR T1 - Validation of tissue microarray immunohistochemistry staining and interpretation in diffuse large B-cell lymphoma AN - 20089464; 7382422 AB - Tissue microarrays (TMAs) show concordance with whole tissue sections in the immunohistochemical evaluation of tumor cells. However, potential inter- institutional variability among observers and immunohistochemical staining methods has not been fully addressed. We selected 21 cases of diffuse large B- cell lymphoma (DLBCL) to process for TMAs. Immunohistochemical stains were performed in 3 laboratories, and reviewed independently by 3 hematopathologists at the 3 institutions. Stains were scored on a 4-point scale. Statistical analyses of variation in the scoring among observers and among different institutions stains were performed. Stains for CD3, CD10, CD20, BCL-2, BCL-6, MIB-1, and FOX-P1 revealed little variation among observers, with an average 51?- ?82% complete agreement and 82?-?100% agreement? plus or minus ?1 numerical score. The rate of concordance when evaluating most stains performed in different laboratories was also relatively good, with an average of 55?-?72% complete agreement and 70?- ?97% agreement? plus or minus ?1 score. However, scoring of MUM-1 and p53 stains showed wider variation, with an average of only 37 and 30% complete agreement among observers, and 11 and 45% agreement when stains from different institutions were examined. Further statistical analyses were performed to compare the observers scoring of their own institutions stains (self-review) vs. observers scoring of other institutions stains (non-self). The agreement rate for the p53 stain was significantly higher when based on self-review (average 58% complete agreement) compared with an agreement rate of only 10.5% when based on a review of stains performed in another laboratory, non-self review, P ?-?0.01. This difference in the self- vs. non-self review was not seen when data for MUM-1 were analysed. In conclusion, most phenotypic markers used in the analysis of DLBCL can be evaluated in TMAs with adequate agreement among observers and laboratories. These include CD3, CD20, CD10, BCL-2, BCL-6, MIB-1, and FOX-P1. However, some markers, such as p53 and MUM-1, are more prone to inter- institutional variation. Variations in interpretation can be partially overcome by self-adjusted/adapt tendency, as seen with p53. Especially with newly developed markers, such as MUM-1, the development of standardized techniques for staining and interpretation is critical to reduce inter-observer variability. JF - Leukemia & Lymphoma AU - Zu, Youli AU - Steinberg, Seth AU - Campo, Elias AU - Hans, Christine AU - Weisenburger, Dennis AU - Braziel, Rita AU - Delabie, Jan AU - Gascoyne, Randy AU - Muller-Hermlink, Konrad AU - Pittaluga, Stefania AU - Raffeld, Mark AU - Chan, Wing AU - Jaffe, Elaine AD - Hematopathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 693 EP - 701 PB - Taylor & Francis Ltd., 11 New Fetter Lane London EC4P 4EE UK, [mailto:info@tandf.co.uk], [URL:http://www.tandf.co.uk] VL - 46 IS - 5 SN - 1042-8194, 1042-8194 KW - Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - Validation KW - monoclonal antibodies KW - tissue microarray KW - immunohistochemistry KW - diffuse large B-cell lymphoma KW - prognostic markers KW - B-cell lymphoma KW - double prime B-cell lymphoma KW - Data processing KW - Statistical analysis KW - Stains KW - Tumor cells KW - p53 protein KW - Leukemia KW - Bcl-6 protein KW - Reviews KW - CD20 antigen KW - Bcl-2 protein KW - CD3 antigen KW - Immunohistochemistry KW - F 06915:Cancer Immunology KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20089464?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Leukemia+%26+Lymphoma&rft.atitle=Validation+of+tissue+microarray+immunohistochemistry+staining+and+interpretation+in+diffuse+large+B-cell+lymphoma&rft.au=Zu%2C+Youli%3BSteinberg%2C+Seth%3BCampo%2C+Elias%3BHans%2C+Christine%3BWeisenburger%2C+Dennis%3BBraziel%2C+Rita%3BDelabie%2C+Jan%3BGascoyne%2C+Randy%3BMuller-Hermlink%2C+Konrad%3BPittaluga%2C+Stefania%3BRaffeld%2C+Mark%3BChan%2C+Wing%3BJaffe%2C+Elaine&rft.aulast=Zu&rft.aufirst=Youli&rft.date=2005-05-01&rft.volume=46&rft.issue=5&rft.spage=693&rft.isbn=&rft.btitle=&rft.title=Leukemia+%26+Lymphoma&rft.issn=10428194&rft_id=info:doi/10.1080%2F10428190500051844 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-05-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - B-cell lymphoma; Data processing; double prime B-cell lymphoma; Statistical analysis; Stains; Tumor cells; p53 protein; Leukemia; Bcl-6 protein; Reviews; CD20 antigen; CD3 antigen; Bcl-2 protein; Immunohistochemistry DO - http://dx.doi.org/10.1080/10428190500051844 ER - TY - JOUR T1 - Effect of Human Stem Cells Labeled with Ferumoxides-Poly-L-lysine on Hematologic and Biochemical Measurements in Rats AN - 19929920; 6276670 AB - PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner. [copy ] RSNA, 2005 JF - Radiology AU - Yocum, Gene T AU - Wilson, Lindsey B AU - Ashari, Parwana AU - Jordan, EKay AU - Frank, Joseph A AU - Arbab, Ali S AD - Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, Md Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 547 EP - 552 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 235 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts KW - Hemoglobin KW - Blood KW - Stem cells KW - Intravenous administration KW - Alkaline phosphatase KW - Renal function KW - Aspartate aminotransferase KW - Liver KW - Bilirubin KW - Mesenchyme KW - Iron KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19929920?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Effect+of+Human+Stem+Cells+Labeled+with+Ferumoxides-Poly-L-lysine+on+Hematologic+and+Biochemical+Measurements+in+Rats&rft.au=Yocum%2C+Gene+T%3BWilson%2C+Lindsey+B%3BAshari%2C+Parwana%3BJordan%2C+EKay%3BFrank%2C+Joseph+A%3BArbab%2C+Ali+S&rft.aulast=Yocum&rft.aufirst=Gene&rft.date=2005-05-01&rft.volume=235&rft.issue=2&rft.spage=547&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-07-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Hemoglobin; Blood; Intravenous administration; Stem cells; Alkaline phosphatase; Renal function; Aspartate aminotransferase; Liver; Bilirubin; Mesenchyme; Iron ER - TY - JOUR T1 - Long CGG-repeat tracts are toxic to human cells: Implications for carriers of Fragile X premutation alleles AN - 19703331; 7493404 AB - People with 59-200 CGG·CCG-repeats in the 5' UTR of one of their FMR1 genes are at risk for Fragile X tremor and ataxia syndrome. Females are also at risk for premature ovarian failure. These symptoms are thought to be due to the presence of the repeats at the DNA and/or RNA level. We show here that long transcribed but untranslated CGG-repeat tracts are toxic to human cells and alter the expression of a wide variety of different genes including caspase-8, CYFIP, Neurotensin and UBE3A. JF - FEBS Letters AU - Handa, Vaishali AU - Goldwater, Deena AU - Stiles, David AU - Cam, Margaret AU - Poy, George AU - Kumari, Daman AU - Usdin, Karen AD - Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, 8 Center Drive MSC 0830, Bethesda, MD 20892-0830, United States, ku@helix.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2702 EP - 2708 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 579 IS - 12 SN - 0014-5793, 0014-5793 KW - Toxicology Abstracts; Genetics Abstracts; CSA Neurosciences Abstracts KW - Fragile X syndrome KW - CGG-repeat KW - Premutation KW - POF KW - Fragile X tremor and ataxia syndrome KW - Risk assessment KW - Neurotensin KW - RNA KW - Caspase-8 KW - Ataxia KW - DNA KW - tremor KW - fragile X mental retardation protein KW - Repeated DNA sequences KW - Ubiquitin-protein ligase KW - X 24490:Other KW - N3 11027:Neurology & neuropathology KW - G 07700:Molecular Genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19703331?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+Letters&rft.atitle=Long+CGG-repeat+tracts+are+toxic+to+human+cells%3A+Implications+for+carriers+of+Fragile+X+premutation+alleles&rft.au=Handa%2C+Vaishali%3BGoldwater%2C+Deena%3BStiles%2C+David%3BCam%2C+Margaret%3BPoy%2C+George%3BKumari%2C+Daman%3BUsdin%2C+Karen&rft.aulast=Handa&rft.aufirst=Vaishali&rft.date=2005-05-01&rft.volume=579&rft.issue=12&rft.spage=2702&rft.isbn=&rft.btitle=&rft.title=FEBS+Letters&rft.issn=00145793&rft_id=info:doi/10.1016%2Fj.febslet.2005.04.004 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-08-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Risk assessment; Neurotensin; RNA; Caspase-8; DNA; Ataxia; fragile X mental retardation protein; tremor; Repeated DNA sequences; Ubiquitin-protein ligase DO - http://dx.doi.org/10.1016/j.febslet.2005.04.004 ER - TY - JOUR T1 - Standardizing global gene expression analysis between laboratories and across platforms AN - 19524308; 7924058 AB - To facilitate collaborative research efforts between multi-investigator teams using DNA microarrays, we identified sources of error and data variability between laboratories and across microarray platforms, and methods to accommodate this variability. RNA expression data were generated in seven laboratories, which compared two standard RNA samples using 12 microarray platforms. At least two standard microarray types (one spotted, one commercial) were used by all laboratories. Reproducibility for most platforms within any laboratory was typically good, but reproducibility between platforms and across laboratories was generally poor. Reproducibility between laboratories increased markedly when standardized protocols were implemented for RNA labeling, hybridization, microarray processing, data acquisition and data normalization. Reproducibility was highest when analysis was based on biological themes defined by enriched Gene Ontology (GO) categories. These findings indicate that microarray results can be comparable across multiple laboratories, especially when a common platform and set of procedures are used. JF - Nature Methods AU - Weis, B K AD - A list of authors and their affiliations appears in the Supplementary Note online., weis@niehs.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 351 EP - 356 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 2 IS - 5 SN - 1548-7091, 1548-7091 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - DNA microarrays KW - Data acquisition KW - Data processing KW - Gene expression KW - RNA KW - W 30900:Methods KW - G 07700:Molecular Genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19524308?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Methods&rft.atitle=Standardizing+global+gene+expression+analysis+between+laboratories+and+across+platforms&rft.au=Weis%2C+B+K&rft.aulast=Weis&rft.aufirst=B&rft.date=2005-05-01&rft.volume=2&rft.issue=5&rft.spage=351&rft.isbn=&rft.btitle=&rft.title=Nature+Methods&rft.issn=15487091&rft_id=info:doi/10.1038%2Fnmeth754 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2013-02-22 N1 - SubjectsTermNotLitGenreText - Gene expression; Data processing; RNA; DNA microarrays; Data acquisition DO - http://dx.doi.org/10.1038/nmeth754 ER - TY - JOUR T1 - A novel method to improve prenatal diagnosis of abnormal systemic venous connections using three- and four-dimensional ultrasonography and 'inversion mode' AN - 19436584; 6786704 AB - Objective: The precise prenatal diagnosis of abnormal venous connections of the fetal heart is challenging. Anatomical accuracy may be important in determining the best route for postnatal angiography, as well as the prognosis and treatment. This study was designed to determine the value of 'inversion mode', a new three- and four-dimensional (4D) rendering algorithm, in the visualization of the spatial relationships of an interrupted inferior vena cava (IVC) with azygos or hemiazygos vein continuation associated with and without heterotaxic syndromes. Methods: Heart volumes were acquired using 4D ultrasonography and spatiotemporal image correlation in cases of interrupted IVC with azygos/hemiazygos continuation (n = 3). Volume datasets were rendered using the 'inversion mode' algorithm and abnormal images were compared to those generated from a library of normal fetuses. Results: The 'inversion mode' rendering algorithm allowed the visualization of dilated azygos or hemiazygos veins and their spatial relationships with the descending aorta, the aortic arch, the superior vena cava, and the atria in cases of interrupted IVC with and without heterotaxic syndromes. Conclusions: The 'inversion mode' algorithm improves prenatal visualization of both dilated azygos and hemiazygos veins, as well as their spatial relationships with the surrounding vascular structures. This has implications for the accurate prenatal diagnosis and management of neonates with abnormal systemic venous connections. JF - Ultrasound in Obstetrics and Gynecology AU - Espinoza, J AU - Goncalves, L F AU - Lee, W AU - Mazor, M AU - Romero, R AD - Perinatology Research Branch, NICHD, NIH, DHHS, Wayne State University/Hutzel Women's Hospital, 3990 John R, 4th Floor, Detroit, MI 48201, USA, warfiela@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 428 EP - 434 PB - John Wiley & Sons, Ltd. VL - 25 IS - 5 SN - 0960-7692, 0960-7692 KW - Biotechnology and Bioengineering Abstracts KW - Heart KW - Gynecology KW - Aorta KW - Prognosis KW - Algorithms KW - Prenatal diagnosis KW - Ultrasonography KW - Fetuses KW - Angiography KW - Veins KW - Aortic arch KW - Inversion KW - Neonates KW - Obstetrics KW - Ultrasound KW - Vascular system KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19436584?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Ultrasound+in+Obstetrics+and+Gynecology&rft.atitle=A+novel+method+to+improve+prenatal+diagnosis+of+abnormal+systemic+venous+connections+using+three-+and+four-dimensional+ultrasonography+and+%27inversion+mode%27&rft.au=Espinoza%2C+J%3BGoncalves%2C+L+F%3BLee%2C+W%3BMazor%2C+M%3BRomero%2C+R&rft.aulast=Espinoza&rft.aufirst=J&rft.date=2005-05-01&rft.volume=25&rft.issue=5&rft.spage=428&rft.isbn=&rft.btitle=&rft.title=Ultrasound+in+Obstetrics+and+Gynecology&rft.issn=09607692&rft_id=info:doi/10.1002%2Fuog.1877 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Inversion; Algorithms; Prenatal diagnosis; Veins; Ultrasonography; Fetuses; Heart; Neonates; Aortic arch; Ultrasound; Gynecology; Angiography; Obstetrics; Aorta; Vascular system; Prognosis DO - http://dx.doi.org/10.1002/uog.1877 ER - TY - JOUR T1 - Simultaneous perfusion and blood-oxygenation-level-dependent measurements using single-shot interleaved z-shim echo-planar imaging AN - 19430100; 6486294 AB - Single-shot interleaved z-shim EPI (SSIZS-EPI) was extended to a simultaneous perfusion and blood-oxygenation-level-dependent (BOLD) imaging technique that reduces susceptibility-induced signal loss while preserving rapid image acquisition. Experiments on human brains showed that images acquired with this technique had improved signal-to-noise ratio in the inferior prefrontal, meso-, and lateral-temporal lobes compared with a conventional EPI. Perfusion maps obtained from the SSIZS-EPI images at resting state illustrated substantial signal recovery in these brain areas. Perfusion and BOLD images collected with a sensorimotor paradigm demonstrated the feasibility of the technique to simultaneously measure cerebral blood flow and blood oxygenation signals associated with brain activation. Functional experiments with a neuropsychiatric paradigm showed increased brain activities in the periamygdalar regions in both perfusion and BOLD maps, consistent with a previous H sub(2) super(15)O PET study. The proposed technique, with its advantages of reducing susceptibility artifacts and fast scanning speed, would be useful for obtaining more reliable measurements of functional signals, particularly in the brain regions with field inhomogeneities. JF - Magnetic Resonance in Medicine AU - Yang, Yihong AU - Gu, Hong AU - Silbersweig, David A AU - Stern, Emily AD - Neuroimaging Research Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Building C, Room 383, Baltimore, MD 21042, USA, yihongyang@intra.nida.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1207 EP - 1211 PB - John Wiley & Sons, Ltd. VL - 53 IS - 5 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - sensorimotor system KW - Neuroimaging KW - Perfusion KW - Scanning KW - Functional magnetic resonance imaging KW - Magnetic resonance imaging KW - Positron emission tomography KW - Brain KW - Cerebral blood flow KW - Gene mapping KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19430100?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Simultaneous+perfusion+and+blood-oxygenation-level-dependent+measurements+using+single-shot+interleaved+z-shim+echo-planar+imaging&rft.au=Yang%2C+Yihong%3BGu%2C+Hong%3BSilbersweig%2C+David+A%3BStern%2C+Emily&rft.aulast=Yang&rft.aufirst=Yihong&rft.date=2005-05-01&rft.volume=53&rft.issue=5&rft.spage=1207&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20431 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Brain; Perfusion; Neuroimaging; Gene mapping; Scanning; sensorimotor system; Cerebral blood flow; Positron emission tomography; Magnetic resonance imaging; Functional magnetic resonance imaging DO - http://dx.doi.org/10.1002/mrm.20431 ER - TY - JOUR T1 - RESTORE: Robust estimation of tensors by outlier rejection AN - 19430041; 6486276 AB - Signal variability in diffusion-weighted imaging (DWI) is influenced by both thermal noise and spatially and temporally varying artifacts such as subject motion and cardiac pulsation. In this paper, the effects of DWI artifacts on estimated tensor values, such as trace and fractional anisotropy, are analyzed using Monte Carlo simulations. A novel approach for robust diffusion tensor estimation, called RESTORE (for robust estimation of tensors by outlier rejection), is proposed. This method uses iteratively reweighted least-squares regression to identify potential outliers and subsequently exclude them. Results from both simulated and clinical diffusion data sets indicate that the RESTORE method improves tensor estimation compared to the commonly used linear and nonlinear least-squares tensor fitting methods and a recently proposed method based on the German-McClure M-estimator. The RESTORE method could potentially remove the need for cardiac gating in DWI acquisitions and should be applicable to other MR imaging techniques that use univariate or multivariate regression to fit MRI data to a model. JF - Magnetic Resonance in Medicine AU - Chang, Lin-Ching AU - Jones, Derek K AU - Pierpaoli, Carlo AD - National Institutes of Health, Building 13, Room 3W16, 13 South Drive, Bethesda, MD 20892-5772, USA, cp1a@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1088 EP - 1095 PB - John Wiley & Sons, Ltd. VL - 53 IS - 5 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Monte Carlo simulation KW - Heart KW - Anisotropy KW - Gating KW - Magnetic resonance imaging KW - Regression analysis KW - Diffusion KW - N.M.R. KW - Models KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19430041?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=RESTORE%3A+Robust+estimation+of+tensors+by+outlier+rejection&rft.au=Chang%2C+Lin-Ching%3BJones%2C+Derek+K%3BPierpaoli%2C+Carlo&rft.aulast=Chang&rft.aufirst=Lin-Ching&rft.date=2005-05-01&rft.volume=53&rft.issue=5&rft.spage=1088&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.20426 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Heart; Magnetic resonance imaging; Models; N.M.R.; Diffusion; Regression analysis; Monte Carlo simulation; Anisotropy; Gating DO - http://dx.doi.org/10.1002/mrm.20426 ER - TY - JOUR T1 - Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects AN - 19424409; 6474789 AB - A new recombinant protein (NIE) for immunodiagnosis of human Strongyloides infection has 13% to 18% amino acid identity with antigen 5 insect venom allergen, but the C-terminal segment of NIE showed highest identity with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as bands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay. JF - American Journal of Tropical Medicine and Hygiene AU - Ravi, V AU - King, T P AU - Andersen, J F AU - Nutman, T B AU - Neva, F A AD - LPD, NIAID, 4 Center Drive, Room 4/126, NIH, Bethesda, MD 20892, USA, fneva@niaid.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 549 EP - 553 VL - 72 IS - 5 SN - 0002-9637, 0002-9637 KW - Hornets KW - Paper wasps KW - Potter wasps KW - Yellowjackets KW - Toxicology Abstracts; Entomology Abstracts KW - Western blotting KW - Enzyme-linked immunosorbent assay KW - Amino acids KW - Vespidae KW - Infection KW - Immunodiagnosis KW - Strongyloides stercoralis KW - Polistes KW - Allergens KW - Immunoglobulin E KW - Immunoglobulin G KW - Venom KW - Epitopes KW - Z 05350:Medical, Veterinary, and Agricultural Entomology KW - X 24173:Animals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19424409?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Tropical+Medicine+and+Hygiene&rft.atitle=Strongyloides+stercoralis+recombinant+NIE+antigen+shares+epitope+with+recombinant+Ves+v+5+and+Pol+a+5+allergens+of+insects&rft.au=Ravi%2C+V%3BKing%2C+T+P%3BAndersen%2C+J+F%3BNutman%2C+T+B%3BNeva%2C+F+A&rft.aulast=Ravi&rft.aufirst=V&rft.date=2005-05-01&rft.volume=72&rft.issue=5&rft.spage=549&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Tropical+Medicine+and+Hygiene&rft.issn=00029637&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Immunodiagnosis; Western blotting; Enzyme-linked immunosorbent assay; Amino acids; Immunoglobulin E; Allergens; Immunoglobulin G; Infection; Venom; Epitopes; Strongyloides stercoralis; Polistes; Vespidae ER - TY - JOUR T1 - Partial Redirection of Transgenic Human Growth Hormone Secretion from Rat Salivary Glands AN - 17886909; 6258662 AB - Regulated secretory pathway proteins, when delivered as transgenes to salivary glands, are secreted pre-dominantly into saliva. This is not useful for those proteins whose therapeutic function is required systemically, for example, human growth hormone (hGH). One strategy to improve the efficiency of hGH secretion into the bloodstream involves manipulation of existing sorting signals. The C terminus of hGH is highly conserved and contains a domain similar to the regulated pathway sorting domain of pro-opiomelanocortin (POMC). We hypothesized that, similar to POMC, mutation of this domain would divert hGH secretion from the regulated to the constitutive pathway, which in salivary glands leads to the bloodstream. Several mutations were made in the C terminus of the hGH cDNA and tested in vitro. One biologically active mutant containing E174A and E186A substitutions, and with an included C-terminal extension, was studied in greater detail. Compared with wild-type hGH, we found that this mutant hGH accumulated in the Golgi/trans-Golgi network and showed increased basal secretion in AtT20 cells, a model endocrine cell line. Importantly, in vivo, the mutant hGH displayed a relative increase in the proportion of constitutive pathway secretion seen from rat salivary glands, with a significantly lower saliva-versus-serum secretion ratio (p = 0.03). Although this mutant is unlikely to be therapeutically beneficial, these results suggest that the final destination of a transgenic secretory protein may be controlled by reengineering its sorting determinants. JF - Human Gene Therapy AU - Wang, J AU - Cawley, N X AU - Voutetakis, A AU - Rodriguez, Y M AU - Goldsmith, C M AU - Nieman, L K AU - Hoque, ATMS AU - Frank, S J AU - Snell, C R AU - Loh, Y P AU - Baum, B J AD - Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Building 10, Room 1N113, MSC-1190, Bethesda, MD 20892, USA, bbaum@dir.nidcr.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 571 EP - 583 VL - 16 IS - 5 SN - 1043-0342, 1043-0342 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - Golgi apparatus KW - Growth hormone KW - Gene therapy KW - Proopiomelanocortin KW - Secretion KW - Animal models KW - Cell culture KW - Salivary gland KW - Saliva KW - Mutation KW - W 30965:Miscellaneous, Reviews KW - W3 33180:Gene based (protocols, clinical trials, and animal models) KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17886909?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Human+Gene+Therapy&rft.atitle=Partial+Redirection+of+Transgenic+Human+Growth+Hormone+Secretion+from+Rat+Salivary+Glands&rft.au=Wang%2C+J%3BCawley%2C+N+X%3BVoutetakis%2C+A%3BRodriguez%2C+Y+M%3BGoldsmith%2C+C+M%3BNieman%2C+L+K%3BHoque%2C+ATMS%3BFrank%2C+S+J%3BSnell%2C+C+R%3BLoh%2C+Y+P%3BBaum%2C+B+J&rft.aulast=Wang&rft.aufirst=J&rft.date=2005-05-01&rft.volume=16&rft.issue=5&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Human+Gene+Therapy&rft.issn=10430342&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Growth hormone; Secretion; Salivary gland; Proopiomelanocortin; Mutation; Animal models; Saliva; Cell culture; Golgi apparatus; Gene therapy ER - TY - JOUR T1 - Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Production Significantly Increases during Tricarboxylic Acid Cycle Stress AN - 17875150; 6245838 AB - Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing Staphylococcus epidermidis with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production. JF - Journal of Bacteriology AU - Vuong, Cuong AU - Kidder, Joshua B AU - Jacobson, Erik R AU - Otto, Michael AU - Proctor, Richard A AU - Somerville, Greg A AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, Montana 59840. Departments of Medical Microbiology and Immunology. Medicine, University of Wisconsin Medical School, 1300 University Ave., Madison, Wisconsin 53706. University of Nebraska-Lincoln, Department of Biochemistry, Beadle Center, University of Nebraska, Lincoln, Nebraska 68588-0664. University of Nebraska-Lincoln, Department of Veterinary and Biomedical Sciences, 120 VBS Fair St., Lincoln, Nebraska 68583-0905 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 2967 EP - 2973 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 9 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology KW - Growth rate KW - Adhesins KW - Glucose KW - Intracellular levels KW - Stress KW - ATP KW - Polysaccharides KW - Salts KW - Oxygen KW - Heat KW - Environmental changes KW - Biofilms KW - Tricarboxylic acid cycle KW - Environmental conditions KW - Staphylococcus epidermidis KW - Ethanol KW - J 02722:Biodegradation, growth, nutrition and leaching UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17875150?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Staphylococcus+epidermidis+Polysaccharide+Intercellular+Adhesin+Production+Significantly+Increases+during+Tricarboxylic+Acid+Cycle+Stress&rft.au=Vuong%2C+Cuong%3BKidder%2C+Joshua+B%3BJacobson%2C+Erik+R%3BOtto%2C+Michael%3BProctor%2C+Richard+A%3BSomerville%2C+Greg+A&rft.aulast=Vuong&rft.aufirst=Cuong&rft.date=2005-05-01&rft.volume=187&rft.issue=9&rft.spage=2967&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Growth rate; Adhesins; Intracellular levels; Glucose; ATP; Stress; Polysaccharides; Oxygen; Salts; Heat; Environmental changes; Biofilms; Environmental conditions; Tricarboxylic acid cycle; Ethanol; Staphylococcus epidermidis ER - TY - JOUR T1 - Expression systems for cloned xenobiotic transporters AN - 17874378; 6250369 AB - One challenge of modern biology is to be able to match genes and their encoded proteins with events at the molecular, cellular, tissue, and organism levels, and thus, provide a multi-level understanding of gene function and dysfunction. How well this can be done for xenobiotic transporters depends on a knowledge of the genes expressed in the tissue, the cellular locations of the gene products (do they function for uptake or efflux?), and our ability to match substrates with transporters using information obtained from cloned transporters functioning in heterologous expression systems. Clearly, making a rational choice of expression system to use for the characterization and study of cloned xenobiotic transporters is a critical part of study design. This choice requires well-defined goals, as well as an understanding of the strengths and weaknesses of candidate expression systems. JF - Toxicology and Applied Pharmacology AU - Pritchard, J B AU - Miller, D S AD - Laboratory of Pharmacology and Chemistry, NIH/National Institute of Environmental Health Sciences, 110 Alexander Drive, MD F1-03, Research Triangle Park, NC 27709, USA, pritcha3@niehs.nih.gov Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 256 EP - 262 VL - 204 IS - 3 SN - 0041-008X, 0041-008X KW - Toxicology Abstracts KW - Cloning KW - Xenobiotics KW - X 24240:Miscellaneous UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17874378?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicology+and+Applied+Pharmacology&rft.atitle=Expression+systems+for+cloned+xenobiotic+transporters&rft.au=Pritchard%2C+J+B%3BMiller%2C+D+S&rft.aulast=Pritchard&rft.aufirst=J&rft.date=2005-05-01&rft.volume=204&rft.issue=3&rft.spage=256&rft.isbn=&rft.btitle=&rft.title=Toxicology+and+Applied+Pharmacology&rft.issn=0041008X&rft_id=info:doi/10.1016%2Fj.taap.2004.11.018 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Xenobiotics; Cloning DO - http://dx.doi.org/10.1016/j.taap.2004.11.018 ER - TY - JOUR T1 - The role of IFN regulatory factor-3 in the cytotoxic activity of NS-9, a polyinosinic-polycytidylic acid/cationic liposome complex, against tumor cells AN - 17873841; 6278439 AB - NS-9 is a complex of polyinosinic-polycytidylic acid and a novel cationic liposome, LIC-101. The complex has strong cytotoxic activity against tumor cells derived from epithelial or fibroblastic cells. We have investigated the mechanism of the cytotoxic activity of NS-9 using knockdown cells in which the expression of proteins of interest was inhibited by RNA interference. NS-9 showed strong cytotoxic activity against knockdown cells with reduced expression of double-stranded RNA-dependent protein kinase, RNase L, or IFN- alpha / beta receptor, but showed no cytotoxic activity against IFN regulatory factor-3 (IRF3) knockdown cells. In IRF3-knockdown cells, NS-9 also did not induce either the DNA fragmentation or the rRNA degradation observed in negative control cells. We conclude that IRF3 plays a crucial role in the cytotoxic activity of NS-9 against tumor cells, whereas RNA-dependent protein kinase, RNase L, or type I IFNs are not important for its activity. JF - Molecular Cancer Therapeutics AU - Uno, Tomonori AU - Hirabayashi, Kazuko AU - Murai, Masatoshi AU - Yano, Junichi AU - Ozato, Keiko AD - Discovery Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan and Laboratory of Molecular Growth and Regulation, The National Institute of Child Health and Human Development, NIH, Bethesda, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 799 EP - 805 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 4 IS - 5 SN - 1535-7163, 1535-7163 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - ^a-Interferon KW - DNA fragmentation KW - rRNA KW - Cytotoxicity KW - Poly (I:C) KW - RNA-mediated interference KW - Protein kinase KW - Liposomes KW - Tumor cells KW - W3 33374:Antitumor agents KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17873841?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Cancer+Therapeutics&rft.atitle=The+role+of+IFN+regulatory+factor-3+in+the+cytotoxic+activity+of+NS-9%2C+a+polyinosinic-polycytidylic+acid%2Fcationic+liposome+complex%2C+against+tumor+cells&rft.au=Uno%2C+Tomonori%3BHirabayashi%2C+Kazuko%3BMurai%2C+Masatoshi%3BYano%2C+Junichi%3BOzato%2C+Keiko&rft.aulast=Uno&rft.aufirst=Tomonori&rft.date=2005-05-01&rft.volume=4&rft.issue=5&rft.spage=799&rft.isbn=&rft.btitle=&rft.title=Molecular+Cancer+Therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cytotoxicity; Tumor cells; Protein kinase; Liposomes; Poly (I:C); rRNA; RNA-mediated interference; ^a-Interferon; DNA fragmentation ER - TY - JOUR T1 - Mechanisms of Exocrine Pancreatic Toxicity Induced by Oral Treatment with 2,3,7,8-Tetrachlorodibenzo-p-Dioxin in Female Harlan Sprague-Dawley Rats AN - 17871601; 6246903 AB - In previous 2-year studies of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) conducted by the National Toxicology Program on female Harlan Sprague-Dawley rats, acinar-cell vacuolation, atrophy, inflammation, and arteritis developed at high incidence, and a rare occurrence of pancreatic acinar-cell adenomas and carcinomas was noted. In this investigation, we sought to identify the mechanism involved in the early formative stages of acinar-cell lesions. Pancreas from animals treated for 14 and 31 weeks with 100 ng TCDD/kg body weight or corn oil vehicle was examined immunohistochemically and/or morphometrically. Acinar-cell kinetics were analyzed using staining with hematoxylin and eosin and proliferating cell nuclear antigen. Expressions of cytochrome P450 (CYP) 1A1 and aryl hydrocarbon receptor (AhR) were evaluated to assess direct effects of TCDD exposure. The cholecystokinin-A receptor (CCK-A receptor; CCKAR) and duodenal cholecystokinin 8 (CCK) revealed the associations of dioxin treatment with hormonal changes. Amylase localization showed acinar structural changes that could affect enzymatic production. Increased apoptotic activity in acinar cells occurred in 14- and 31-week-treated animals, with an increase in proliferative activity in the latter. Also in the latter, in the vacuolated acinar cells, CYP1A1 was overexpressed, and statistically significant decreases in expressions of AhR, CCKAR, and amylase occurred. The intensity of CCKAR expression increased in nonvacuolated acinar cells; a decrease in the size of CCK-positive epithelial cells occurred in duodenum. Our findings indicate that dioxin-induced acinar-cell lesions might be related to a direct effect of TCDD on the pancreas. Increase in CYP1A1 and decrease in CCKAR expressions in vacuolated acinar cells may be involved in the pathogenesis of pancreatic lesions. Changes in the expression of CYP or CCKAR may have induced the acinar-cell tumors by initiating proliferation. JF - Toxicological Sciences AU - Yoshizawa, Katsuhiko AU - Marsh, Tiwanda AU - Foley, Julie F AU - Cai, Bo AU - Peddada, Shyamal AU - Walker, Nigel J AU - Nyska, Abraham AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 594 EP - 606 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 85 IS - 1 SN - 1096-6080, 1096-6080 KW - Toxicology Abstracts KW - Epithelial cells KW - Apoptosis KW - Cholecystokinin KW - Duodenum KW - Pancreas KW - Statistical analysis KW - TCDD KW - Toxicity KW - Proliferating cell nuclear antigen KW - Acinar cells KW - Inflammation KW - Carcinoma KW - Body weight KW - Atrophy KW - Cytochrome P450 KW - Aryl hydrocarbon receptors KW - Dioxin KW - X 24154:Pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17871601?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+Sciences&rft.atitle=Mechanisms+of+Exocrine+Pancreatic+Toxicity+Induced+by+Oral+Treatment+with+2%2C3%2C7%2C8-Tetrachlorodibenzo-p-Dioxin+in+Female+Harlan+Sprague-Dawley+Rats&rft.au=Yoshizawa%2C+Katsuhiko%3BMarsh%2C+Tiwanda%3BFoley%2C+Julie+F%3BCai%2C+Bo%3BPeddada%2C+Shyamal%3BWalker%2C+Nigel+J%3BNyska%2C+Abraham&rft.aulast=Yoshizawa&rft.aufirst=Katsuhiko&rft.date=2005-05-01&rft.volume=85&rft.issue=1&rft.spage=594&rft.isbn=&rft.btitle=&rft.title=Toxicological+Sciences&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - TCDD; Acinar cells; Cytochrome P450; Epithelial cells; Toxicity; Duodenum; Atrophy; Proliferating cell nuclear antigen; Apoptosis; Aryl hydrocarbon receptors; Statistical analysis; Cholecystokinin; Carcinoma; Pancreas; Dioxin; Inflammation; Body weight ER - TY - JOUR T1 - SspA is required for acid resistance in stationary phase by downregulation of H-NS in Escherichia coli AN - 17866552; 6230247 AB - The stringent starvation protein A (SspA) is a RNA polymerase-associated protein and is required for transcriptional activation of bacteriophage P1 late promoters. However, the role of SspA in gene expression in Escherichia coli is essentially unknown. In this work, we show that SspA is essential for cell survival during acid-induced stress. Apparently, SspA inhibits stationary-phase accumulation of H-NS, a global regulator which functions mostly as a repressor, thereby derepressing multiple stress defence systems including those for acid stress and nutrient starvation. Consequently, the gene expression pattern of the H-NS regulon is altered in the sspA mutant, leading to acid-sensitive and hypermotile phenotypes. Thus, our study indicates that SspA is a global regulator, which acts upstream of H-NS, and thereby plays an important role in the stress response of E. coli during stationary phase. In addition, our results indicate that the expression of the H-NS regulon is sensitive to small changes in the cellular level of H-NS, enabling the cell to response rapidly to environment cues. As SspA and H-NS are highly conserved among Gram-negative bacteria, of which many are pathogenic, the global role of SspA in the stress response and pathogenesis is discussed. JF - Molecular Microbiology AU - Hansen, Anne-Marie AU - Qiu, Yu AU - Yeh, Norman AU - Blattner, Frederick R AU - Durfee, Tim AU - Jin, Ding Jun AD - Transcription Control Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, NIH, Bldg. 469, PO Box B, Frederick, MD 21702, USA, djjin@helix.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 719 EP - 734 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 56 IS - 3 SN - 0950-382X, 0950-382X KW - sspA protein KW - Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - Phages KW - Cell survival KW - Nutrients KW - Gene expression KW - stationary phase KW - Promoters KW - Gram-negative bacteria KW - Escherichia coli KW - Starvation KW - Stress KW - RNA KW - Repressors KW - Transcription activation KW - J 02722:Biodegradation, growth, nutrition and leaching KW - N 14080:Other UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17866552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Microbiology&rft.atitle=SspA+is+required+for+acid+resistance+in+stationary+phase+by+downregulation+of+H-NS+in+Escherichia+coli&rft.au=Hansen%2C+Anne-Marie%3BQiu%2C+Yu%3BYeh%2C+Norman%3BBlattner%2C+Frederick+R%3BDurfee%2C+Tim%3BJin%2C+Ding+Jun&rft.aulast=Hansen&rft.aufirst=Anne-Marie&rft.date=2005-05-01&rft.volume=56&rft.issue=3&rft.spage=719&rft.isbn=&rft.btitle=&rft.title=Molecular+Microbiology&rft.issn=0950382X&rft_id=info:doi/10.1111%2Fj.1365-2958.2005.04567.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - SuppNotes - Figures, 9; tables, 1; references, 104. N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Stress; stationary phase; Gene expression; Starvation; Promoters; Transcription activation; Cell survival; Gram-negative bacteria; Repressors; Phages; Nutrients; RNA DO - http://dx.doi.org/10.1111/j.1365-2958.2005.04567.x ER - TY - JOUR T1 - Occupation and risk of stomach cancer in Poland AN - 17847159; 6246602 AB - BACKGROUND: In spite of the dramatic decline in the incidence of stomach cancer in the twentieth century, Poland has one of the highest rates in the world. AIMS: To evaluate the risk of stomach cancer by grouped occupations and industries, as well as by some specific occupational exposures. METHODS: Cases (n = 443) were newly diagnosed with stomach adenocarcinomas between 1994 and 1996. Controls (n = 479) were randomly selected from the general population in Warsaw. RESULTS: Only a few occupations and industries were associated with significantly increased risks of stomach cancer. The most suggestive finding was for work in the leather goods industry. Risk was also significantly increased among men working in fabricated metal production and among women ever employed as managers and governmental officials. Men ever employed as teaching professionals and women employed as technical and science professionals had significantly decreased risks of stomach cancer. Among men, a significant positive trend in risk with duration of employment was observed for work in the leather industry and special trade construction. No significantly increased risks were observed for specific exposures assessed by a job-exposure matrix or by self-reports. However among men there were non-significantly increased risks with 10 or more years exposure to asbestos, metal dust, and nitrosamines assessed by a job-exposure matrix. CONCLUSIONS: Employment in the leather goods industry, special trade construction, and metal fabrication was associated with an increased risk of stomach cancer among men. However, there were only weak associations with specific exposures. Occupational exposures do not contribute substantially to the high rates of stomach cancer in Poland. JF - Occupational and Environmental Medicine AU - Krstev, S AU - Dosemeci, M AU - Lissowska, J AU - Chow, W-H AU - Zatonski, W AU - Ward, M H AD - Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 318 EP - 324 PB - B M J Publishing Group, B.M.A. House Tavistock Sq. London WC1H 9JR UK VL - 62 IS - 5 SN - 1351-0711, 1351-0711 KW - stomach KW - Health & Safety Science Abstracts; Risk Abstracts KW - Dust KW - Nitrosamines KW - Construction industry KW - Occupational exposure KW - Metals KW - Asbestos KW - Cancer KW - Poland KW - R2 23080:Industrial and labor KW - H 1000:Occupational Safety and Health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17847159?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Occupational+and+Environmental+Medicine&rft.atitle=Occupation+and+risk+of+stomach+cancer+in+Poland&rft.au=Krstev%2C+S%3BDosemeci%2C+M%3BLissowska%2C+J%3BChow%2C+W-H%3BZatonski%2C+W%3BWard%2C+M+H&rft.aulast=Krstev&rft.aufirst=S&rft.date=2005-05-01&rft.volume=62&rft.issue=5&rft.spage=318&rft.isbn=&rft.btitle=&rft.title=Occupational+and+Environmental+Medicine&rft.issn=13510711&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Poland; Cancer; Occupational exposure; Metals; Dust; Nitrosamines; Construction industry; Asbestos ER - TY - JOUR T1 - Parent-imposed limits on high-risk adolescent driving: are they stricter with graduated driver licensing? AN - 17813998; 6213940 AB - The purpose of this study was to determine whether parent-imposed limits on 16-year-old high-risk driving are stricter in Maryland (MD), a state with graduated driver licensing (GDL) than in Connecticut (CT), a non-GDL state. In both states, parents and adolescents completed telephone surveys about the restrictions that parents placed on their adolescents' driving at night, with adolescent passengers, and at high speeds. In Maryland, surveys took place 1 month (294 parent-adolescent pairs) and 4 months (292 parent-adolescent pairs) after provisional licensure. In Connecticut, surveys took place the first month (132 pairs) and the third month (108 pairs) after adolescent licensure. The findings indicated that after controlling for demographic characteristics, Maryland parents and adolescents reported stricter parent-imposed limits for adolescent passengers, high-speed roads, weekend night driving, and overall limits. Parents in GDL states appear better able to establish and enforce adolescent driving restrictions when the licensing state stipulates, favors, and supports regulated adolescent driving. JF - Accident Analysis & Prevention AU - Hartos, J L AU - Simons-Morton, B G AU - Beck, KH AU - Leaf, WA AD - Interdisciplinary Health Studies, Minor Barnard 221, Department of Health Behavior & Administration, College of Health & Human Services, UNC Charlotte, 9201 University Blvd, Charlotte, NC 28223-0001, USA, jessica_hartos@nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 557 EP - 562 PB - Elsevier Science Ltd., Pergamon, P.O. Box 800 Kidlington Oxford OX5 1DX UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 37 IS - 3 SN - 0001-4575, 0001-4575 KW - graduated driver licensing KW - Health & Safety Science Abstracts; Risk Abstracts KW - USA, Connecticut KW - Motor vehicles KW - Licensing KW - Accidents KW - driving ability KW - State programs KW - prevention KW - Highways KW - USA, Maryland KW - Adolescents KW - Government programs KW - Traffic safety KW - R2 23020:Technological risks KW - H 2000:Transportation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17813998?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Accident+Analysis+%26+Prevention&rft.atitle=Parent-imposed+limits+on+high-risk+adolescent+driving%3A+are+they+stricter+with+graduated+driver+licensing%3F&rft.au=Hartos%2C+J+L%3BSimons-Morton%2C+B+G%3BBeck%2C+KH%3BLeaf%2C+WA&rft.aulast=Hartos&rft.aufirst=J&rft.date=2005-05-01&rft.volume=37&rft.issue=3&rft.spage=557&rft.isbn=&rft.btitle=&rft.title=Accident+Analysis+%26+Prevention&rft.issn=00014575&rft_id=info:doi/10.1016%2Fj.aap.2005.01.008 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - USA, Connecticut; USA, Maryland; Adolescents; driving ability; Government programs; State programs; Licensing; Accidents; Motor vehicles; Traffic safety; prevention; Highways DO - http://dx.doi.org/10.1016/j.aap.2005.01.008 ER - TY - JOUR T1 - Adjusting Lung Cancer Risks for Temporal and Spatial Variations in Radon Concentration in Dwellings in Gansu Province, China AN - 17651763; 6208402 AB - Our recent study in Gansu Province, China reported an increasing risk of lung cancer with increasing residential radon concentration that was consistent with previous pooled analyses and with meta-analyses of other residential studies (Wang et al., Am. J. Epidemiol.155, 554-564, 2002). Dosimetry used current radon measurements (1-year track-etch detectors) in homes to characterize concentrations for the previous 30 years, resulting in uncertainties in exposure and possibly reduced estimates of disease risk. We conducted a 3-year substudy in 55 houses to model the temporal and spatial variability in radon levels and to adjust estimates of radon risk. Temporal variation represented the single largest source of uncertainty, suggesting the usefulness of multi-year measurements to assess this variation; however, substantial residual variation remained unexplained. The uncertainty adjustment increased estimates of the excess odds ratio by 50-100%, suggesting that residential radon studies using similar dosimetry may also underestimate radon effects. These results have important implications for risk assessment. JF - Radiation Research AU - Lubin, J H AU - Wang, Z Y AU - Wang, L D AU - Boice, J D AU - Cui, H X AU - Zhang AU - Conrath, S AU - Xia, Y AU - Shang, B AU - Cao, J S AU - Kleinerman, R A AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 571 EP - 579 PB - Radiation Research Society VL - 163 IS - 5 SN - 0033-7587, 0033-7587 KW - Toxicology Abstracts KW - X 24210:Radiation & radioactive materials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17651763?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+Research&rft.atitle=Adjusting+Lung+Cancer+Risks+for+Temporal+and+Spatial+Variations+in+Radon+Concentration+in+Dwellings+in+Gansu+Province%2C+China&rft.au=Lubin%2C+J+H%3BWang%2C+Z+Y%3BWang%2C+L+D%3BBoice%2C+J+D%3BCui%2C+H+X%3BZhang%3BConrath%2C+S%3BXia%2C+Y%3BShang%2C+B%3BCao%2C+J+S%3BKleinerman%2C+R+A&rft.aulast=Lubin&rft.aufirst=J&rft.date=2005-05-01&rft.volume=163&rft.issue=5&rft.spage=571&rft.isbn=&rft.btitle=&rft.title=Radiation+Research&rft.issn=00337587&rft_id=info:doi/10.1043%2F0033-7587%282005%291632.0.CO%3B2 L2 - http://journals.allenpress.com/jrnlserv/?request=get-abstract&issn=0033-7587&volume=163&page=571 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-12-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1043/0033-7587(2005)163[0571:ALCRFT]2.0.CO;2 ER - TY - JOUR T1 - A CD8 super(+) T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity against Chlamydia pneumoniae AN - 17624956; 6275613 AB - An intact T cell compartment and IFN- gamma signaling are required for protective immunity against CHLAMYDIA: In the mouse model of Chlamydia pneumoniae (Cpn) infection, this immunity is critically dependent on CD8 super(+) T cells. Recently we reported that Cpn-infected mice generate an MHC class I-restricted CD8 super(+) Tc1 response against various Cpn Ags, and that CD8 super(+) CTL to multiple epitopes inhibit Cpn growth in vitro. Here, we engineered a DNA minigene encoding seven H-2 super(b)-restricted Cpn CTL epitopes, the universal pan-DR epitope Th epitope, and an endoplasmic reticulum-translocating signal sequence. Immunization of C57BL/6 mice with this construct primed IFN- gamma -producing CD8 super(+) CTL against all seven CTL epitopes. CD8 super(+) T cell lines generated to minigene-encoded CTL epitopes secreted IFN- gamma and TNF- alpha and exhibited CTL activity upon recognition of Cpn-infected macrophages. Following intranasal challenge with Cpn, a 3.6 log reduction in mean lung bacterial numbers compared with control animals was obtained. Using a 20-fold increase in the Cpn challenging dose, minigene-vaccinated mice had a 60-fold reduction in lung bacterial loads, compared with controls. Immunization and challenge studies with beta sub(2)-microglobulin super(-/-) mice indicated that the reduction of lung Cpn burdens was mediated by the MHC class I-dependent CD8 super(+) T cells to minigene-included Cpn CTL epitopes, rather than by pan-DR epitope-specific CD4 super(+) T cells. This constitutes the first demonstration of significant protection achieved by immunization with a CD8 super(+) T cell epitope-based DNA construct in a bacterial system and provides the basis for the optimal design of multicomponent anti-Cpn vaccines for humans. JF - Journal of Immunology AU - Pinchuk, Irina AU - Starcher, Barry C AU - Livingston, Brian AU - Tvninnereim, Amy AU - Wu, Shiping AU - Appella, Ettore AU - Sidney, John AU - Sette, Alessandro AU - Wizel, Benjamin AD - Center for Pulmonary and Infectious Disease Control, Departments of Microbiology and Immunology and Biochemistry, University of Texas Health Center, Tyler, TX 75708. Epimmune and La Jolla Institute for Allergy and Immunology, San Diego, CA 92121. National Cancer Institute, Bethesda, MD 20892 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5729 EP - 5739 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - mice KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - J 02834:Vaccination and immunization KW - F 06100:Vaccines - active immunity KW - J 02845:Ear, nose and respiratory tract UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17624956?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=A+CD8+super%28%2B%29+T+Cell+Heptaepitope+Minigene+Vaccine+Induces+Protective+Immunity+against+Chlamydia+pneumoniae&rft.au=Pinchuk%2C+Irina%3BStarcher%2C+Barry+C%3BLivingston%2C+Brian%3BTvninnereim%2C+Amy%3BWu%2C+Shiping%3BAppella%2C+Ettore%3BSidney%2C+John%3BSette%2C+Alessandro%3BWizel%2C+Benjamin&rft.aulast=Pinchuk&rft.aufirst=Irina&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5729&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Dendritic Cells Endocytose Bacillus anthracis Spores: Implications for Anthrax Pathogenesis AN - 17624278; 6275591 AB - Phagocytosis of inhaled Bacillus anthracis spores and subsequent trafficking to lymph nodes are decisive events in the progression of inhalational anthrax because they initiate germination and dissemination of spores. Found in high frequency throughout the respiratory track, dendritic cells (DCs) routinely take up foreign particles and migrate to lymph nodes. However, the participation of DCs in phagocytosis and dissemination of spores has not been investigated previously. We found that human DCs readily engulfed fully pathogenic Ames and attenuated B. anthracis spores predominately by coiling phagocytosis. Spores provoked a loss of tissue-retaining chemokine receptors (CCR2, CCR5) with a concurrent increase in lymph node homing receptors (CCR7, CD11c) on the membrane of DCs. After spore infection, immature DCs displayed a mature phenotype (CD83 super(bright), HLA-DR super(bright), CD80 super(bright), CD86 super(bright), CD40 super(bright)) and enhanced costimulatory activity. Surprisingly, spores activated the MAPK cascade (ERK, p38) within 30 min and stimulated expression of several inflammatory response genes by 2 h. MAPK signaling was extinguished by 6 h infection, and there was a dramatic reduction of secreted TNF- alpha , IL-6, and IL-8 in the absence of DC death. This corresponded temporally with enzymatic cleavage of proximal MAPK signaling proteins (MEK-1, MEK-3, and MAP kinase kinase-4) and may indicate activity of anthrax lethal toxin. Taken together, these results suggest that B. anthracis may exploit DCs to facilitate infection. JF - Journal of Immunology AU - Brittingham, Katherine C AU - Ruthel, Gordon AU - Panchal, Rekha G AU - Fuller, Claudette L AU - Ribot, Wilson J AU - Hoover, Timothy A AU - Young, Howard A AU - Anderson, Arthur O AU - Bavari, Sina AD - U.S. Army Medical Research Institute of Infectious Diseases, and. Developmental Therapeutics Program and. Laboratory of Experimental Immunology, Centre for Cancer Research, National Cancer Institute, Frederick, MD 21702 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5545 EP - 5552 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 174 IS - 9 SN - 0022-1767, 0022-1767 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - J 02833:Immune response and immune mechanisms KW - F 06106:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17624278?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Dendritic+Cells+Endocytose+Bacillus+anthracis+Spores%3A+Implications+for+Anthrax+Pathogenesis&rft.au=Brittingham%2C+Katherine+C%3BRuthel%2C+Gordon%3BPanchal%2C+Rekha+G%3BFuller%2C+Claudette+L%3BRibot%2C+Wilson+J%3BHoover%2C+Timothy+A%3BYoung%2C+Howard+A%3BAnderson%2C+Arthur+O%3BBavari%2C+Sina&rft.aulast=Brittingham&rft.aufirst=Katherine&rft.date=2005-05-01&rft.volume=174&rft.issue=9&rft.spage=5545&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Measurement of Vasoactive Intestinal Peptide using a Competitive Fluorescent Microsphere Immunoassay or ELISA in human blood samples AN - 17623683; 6396017 AB - The concentration of Vasoactive Intestinal Peptide (VIP) as measured by recycling immunoaffinity chromatography (RIC) has been reported to be elevated in the blood of patients with autism as compared with normal subjects. In this study, we have developed a ''Competitive Fluorescent Microsphere Immunoassay'' (cFMI) in which VIP competes with biotinylated VIP in binding to polyclonal antibodies on microspheres. The results were obtained using the Luminex super(1) super(0) super(0) system. We measured VIP in serum, plasma, and material eluted from dried blood spots on filter paper with both the cFMI and an ELISA procedure. We found that a purification procedure was necessary for obtaining useful results from plasma and serum, however, a preincubation step was required with the blood eluates. This newly developed cFMI was more sensitive (2.5 vs. 20.0 pg/ml), and more reproducible than the ELISA. To get accurate measurements of VIP in eluted material high sensitivity is especially important. Thus, the cFMI using the Luminex system has definite advantages over a conventional ELISA including the possibility that samples can be assayed at higher dilutions. We have determined that the VIP concentrations of serum, plasma, and dried blood spot eluate specimens as measured with the cFMI assay system were similar to those measured with ELISA. Thus, the new cFMI using Luminex system may be useful for detection of VIP in human blood samples. JF - Journal of Immunological Methods AU - Song, E Y AU - VanDunk, C AU - Kuddo, T AU - Nelson, P G AD - National Institute of Child Health and Human Development, National Institutes of Health, Building 31 Room 2 A 25, Bethesda, MD 20892-4480, USA, nelsonp@mail.nih.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 63 EP - 73 VL - 300 IS - 1-2 SN - 0022-1759, 0022-1759 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts KW - W3 33240:Immunology KW - F 06704:Immunoassays KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17623683?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunological+Methods&rft.atitle=Measurement+of+Vasoactive+Intestinal+Peptide+using+a+Competitive+Fluorescent+Microsphere+Immunoassay+or+ELISA+in+human+blood+samples&rft.au=Song%2C+E+Y%3BVanDunk%2C+C%3BKuddo%2C+T%3BNelson%2C+P+G&rft.aulast=Song&rft.aufirst=E&rft.date=2005-05-01&rft.volume=300&rft.issue=1-2&rft.spage=63&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunological+Methods&rft.issn=00221759&rft_id=info:doi/10.1016%2Fj.jim.2005.02.009 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-11-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1016/j.jim.2005.02.009 ER - TY - JOUR T1 - A web-based tool for principal component and significance analysis of microarray data AN - 17565788; 6270208 AB - SUMMARY: We have developed a program for microarray data analysis, which features the false discovery rate for testing statistical significance and the principal component analysis using the singular value decomposition method for detecting the global trends of gene-expression patterns. Additional features include analysis of variance with multiple methods for error variance adjustment, correction of cross-channel correlation for two-color microarrays, identification of genes specific to each cluster of tissue samples, biplot of tissues and corresponding tissue-specific genes, clustering of genes that are correlated with each principal component (PC), three-dimensional graphics based on virtual reality modeling language and sharing of PC between different experiments. The software also supports parameter adjustment, gene search and graphical output of results. The software is implemented as a web tool and thus the speed of analysis does not depend on the power of a client computer. AVAILABILITY: The tool can be used on-line or downloaded at http://lgsun.grc.nia.nih.gov/ANOVA/ JF - Bioinformatics AU - Sharov, Alexei A AU - Dudekula, Dawood B AU - Ko, Minoru SH AD - Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health Baltimore, MD 21224, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2548 EP - 2549 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 10 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Computer programs KW - software KW - Statistics KW - Data processing KW - Principal components analysis KW - Computer graphics KW - Language KW - Bioinformatics KW - Computer applications KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17565788?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=A+web-based+tool+for+principal+component+and+significance+analysis+of+microarray+data&rft.au=Sharov%2C+Alexei+A%3BDudekula%2C+Dawood+B%3BKo%2C+Minoru+SH&rft.aulast=Sharov&rft.aufirst=Alexei&rft.date=2005-05-01&rft.volume=21&rft.issue=10&rft.spage=2548&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Computer programs; Data processing; software; Statistics; Bioinformatics; Language; Principal components analysis; Computer applications; Gene expression; Computer graphics ER - TY - JOUR T1 - Characterizing dye bias in microarray experiments AN - 17565093; 6270184 AB - MOTIVATION: Spot intensity serves as a proxy for gene expression in dual-label microarray experiments. Dye bias is defined as an intensity difference between samples labeled with different dyes attributable to the dyes instead of the gene expression in the samples. Dye bias that is not removed by array normalization can introduce bias into comparisons between samples of interest. But if the bias is consistent across samples for the same gene, it can be corrected by proper experimental design and analysis. If the dye bias is not consistent across samples for the same gene, but is different for different samples, then removing the bias becomes more problematic, perhaps indicating a technical limitation to the ability of fluorescent signals to accurately represent gene expression. Thus, it is important to characterize dye bias to determine: (1) whether it will be removed for all genes by array normalization, (2) whether it will not be removed by normalization but can be removed by proper experimental design and analysis and (3) whether dye bias correction is more problematic than either of these and is not easily removable. RESULTS: We analyzed two large (each >27 arrays) tissue culture experiments with extensive dye swap arrays to better characterize dye bias. Indirect, amino-allyl labeling was used in both experiments. We found that post-normalization dye bias that is consistent across samples does appear to exist for many genes, and that controlling and correcting for this type of dye bias in design and analysis is advisable. The extent of this type of dye bias remained unchanged under a wide range of normalization methods (median-centering, various loess normalizations) and statistical analysis techniques (parametric, rank based, permutation based, etc.). We also found dye bias related to the individual samples for a much smaller subset of genes. But these sample-specific dye biases appeared to have minimal impact on estimated gene-expression differences between the cell lines. AVAILABILITY: Supplementary information: http://linus.nci.nih.gov/~brb/TechReport.htm JF - Bioinformatics AU - Dobbin, K K AU - Kawasaki, E S AU - Petersen, D W AU - Simon, R M AD - Biometric Research Branch, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA. Advanced Technology Center, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2430 EP - 2437 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 21 IS - 10 SN - 1367-4803, 1367-4803 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - Gene expression KW - Dyes KW - Statistical analysis KW - Bioinformatics KW - Tissue culture KW - DNA microarrays KW - W 30965:Miscellaneous, Reviews KW - W4 350:Bioinformatics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17565093?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioinformatics&rft.atitle=Characterizing+dye+bias+in+microarray+experiments&rft.au=Dobbin%2C+K+K%3BKawasaki%2C+E+S%3BPetersen%2C+D+W%3BSimon%2C+R+M&rft.aulast=Dobbin&rft.aufirst=K&rft.date=2005-05-01&rft.volume=21&rft.issue=10&rft.spage=2430&rft.isbn=&rft.btitle=&rft.title=Bioinformatics&rft.issn=13674803&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Gene expression; Dyes; Tissue culture; Statistical analysis; Bioinformatics; DNA microarrays ER - TY - JOUR T1 - Synthesis and Characterization of Lipooligosaccharide-Based Conjugate Vaccines for Serotype B Moraxella catarrhalis AN - 17543725; 6266374 AB - Moraxella catarrhalis is an important cause of otitis media in children and respiratory tract infections in the elderly. Lipooligosaccharide (LOS) is a major surface antigen of the bacterium that elicits bactericidal antibodies. Serological studies show that three major LOS types (A, B, and C) have been identified among clinical isolates. Our previous studies demonstrated that the type A LOS-based conjugates were immunogenic in animals. In this study, LOS from type B strain 26397 was detoxified and conjugated to tetanus toxoid (TT) or a cross-reactive mutant (CRM) of diphtheria toxin to form detoxified LOS (dLOS)-TT and dLOS-CRM, respectively, as vaccine candidates. The molar ratios of dLOS to TT and CRM in the conjugates were 43:1 and 19:1, respectively, while both weight ratios were around 0.9. The antigenicity of the conjugates was similar to that of the LOS, as determined by enzyme-linked immunosorbent assay using a rabbit antiserum to strain 26397. Subcutaneous immunization with each conjugate elicited a 180- to 230-fold rise of serum anti-LOS immunoglobulin G in mice and >2,000-fold rise in rabbits. In addition, both mouse and rabbit antisera showed elevated complement-mediated bactericidal activity against the homologous strain, and a representative rabbit antiserum showed bactericidal activity against nine of twelve clinical isolates studied. The bactericidal activity of the rabbit antiserum can be fully inhibited by the type B LOS but not the A or C LOS. These results indicate that the type B LOS-based conjugates can be used as vaccine components for further investigation. JF - Infection and Immunity AU - Yu, Shengqing AU - Gu, Xin-Xing AD - Vaccine Research Facility, National Institute on Deafness and Other Communication Disorders, Rockville, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 2790 EP - 2796 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 5 SN - 0019-9567, 0019-9567 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - Antigenicity KW - Toxoids KW - Tetanus KW - Geriatrics KW - Clinical isolates KW - Enzyme-linked immunosorbent assay KW - Moraxella catarrhalis KW - Immunization KW - Lipooligosaccharides KW - Otitis media KW - Immunoglobulin G KW - Vaccines KW - Serotypes KW - Complement KW - Infection KW - Bactericidal activity KW - Children KW - Diphtheria toxin KW - Respiratory tract diseases KW - Antisera KW - surface antigens KW - W3 33365:Vaccines (other) KW - J 02834:Vaccination and immunization KW - F 06100:Vaccines - active immunity KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17543725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Synthesis+and+Characterization+of+Lipooligosaccharide-Based+Conjugate+Vaccines+for+Serotype+B+Moraxella+catarrhalis&rft.au=Yu%2C+Shengqing%3BGu%2C+Xin-Xing&rft.aulast=Yu&rft.aufirst=Shengqing&rft.date=2005-05-01&rft.volume=73&rft.issue=5&rft.spage=2790&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Moraxella catarrhalis; Vaccines; Bactericidal activity; Clinical isolates; Geriatrics; Lipooligosaccharides; Diphtheria toxin; Complement; Children; surface antigens; Respiratory tract diseases; Otitis media; Serotypes; Enzyme-linked immunosorbent assay; Antisera; Tetanus; Immunoglobulin G; Immunization; Antigenicity; Toxoids; Infection ER - TY - JOUR T1 - Plasmid Partition System of the P1par Family from the pWR100 Virulence Plasmid of Shigella flexneri AN - 17542511; 6266463 AB - P1par family members promote the active segregation of a variety of plasmids and plasmid prophages in gram-negative bacteria. Each has genes for ParA and ParB proteins, followed by a parS partition site. The large virulence plasmid pWR100 of Shigella flexneri contains a new P1par family member: pWR100par. Although typical parA and parB genes are present, the putative pWR100parS site is atypical in sequence and organization. However, pWR100parS promoted accurate plasmid partition in Escherichia coli when the pWR100 Par proteins were supplied. Unique BoxB hexamer motifs within parS define species specificities among previously described family members. Although substantially different from P1parS from the P1 plasmid prophage of E. coli, pWR100parS has the same BoxB sequence. As predicted, the species specificity of the two types proved identical. They also shared partition-mediated incompatibility, consistent with the proposed mechanistic link between incompatibility and species specificity. Among several informative sequence differences between pWR100parS and P1parS is the presence of a 21-bp insert at the center of the pWR100parS site. Deletion of this insert left much of the parS activity intact. Tolerance of central inserts with integral numbers of helical DNA turns reflects the critical topology of these sites, which are bent by binding the host IHF protein. JF - Journal of Bacteriology AU - Sergueev, Kirill AU - Dabrazhynetskaya, Alena AU - Austin, Stuart AD - Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute, NCI-FCRDC, Frederick, Maryland 21702-1201 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3369 EP - 3373 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 187 IS - 10 SN - 0021-9193, 0021-9193 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - hexamers KW - Deletion KW - IHF protein KW - ParB protein KW - Plasmids KW - Prophages KW - Virulence KW - Gram-negative bacteria KW - Shigella flexneri KW - DNA KW - Escherichia coli KW - J 02760:Plasmids KW - G 07203:Plasmids UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17542511?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Bacteriology&rft.atitle=Plasmid+Partition+System+of+the+P1par+Family+from+the+pWR100+Virulence+Plasmid+of+Shigella+flexneri&rft.au=Sergueev%2C+Kirill%3BDabrazhynetskaya%2C+Alena%3BAustin%2C+Stuart&rft.aulast=Sergueev&rft.aufirst=Kirill&rft.date=2005-05-01&rft.volume=187&rft.issue=10&rft.spage=3369&rft.isbn=&rft.btitle=&rft.title=Journal+of+Bacteriology&rft.issn=00219193&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Escherichia coli; Shigella flexneri; Plasmids; Virulence; Prophages; Deletion; hexamers; Gram-negative bacteria; IHF protein; ParB protein; DNA ER - TY - JOUR T1 - Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences AN - 17541084; 6266402 AB - Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated amino acid sequences that were absent in the Lyme disease spirochete Borrelia burgdorferi. The predicted amino acid sequences of these genes (BH0209, BH0512, and BH0553) have hydrophobic N termini, indicating that these proteins may be secreted. B. hermsii transcribed the three ORFs in vitro, and the BH0512- and BH0553-encoded proteins (PBH-512 and PBH-553) were produced in vitro and in experimentally infected mice. PBH-512 and PBH-553 were on the spirochete's outer surface, and antiserum to these proteins reduced the adherence of B. hermsii to red blood cells. PCR analyses of 28 isolates of B. hermsii and 8 isolates of B. turicatae demonstrated polymorphism in each gene correlated with the number of repeats. Serum samples from relapsing fever patients reacted with recombinant PBH-512 and PBH-553, suggesting that these proteins are produced during human infection. These polymorphic proteins may be involved in the pathogenicity of these relapsing fever spirochetes and provide a mechanism for antigenic heterogeneity within their populations. JF - Infection and Immunity AU - Guyard, Cyril AU - Chester, Earl M AU - Raffel, Sandra J AU - Schrumpf, Merry E AU - Policastro, Paul F AU - Porcella, Stephen F AU - Leong, John M AU - Schwan, Tom G AD - Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana. Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, Massachussetts Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 3025 EP - 3037 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 5 SN - 0019-9567, 0019-9567 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology KW - Genomes KW - Borrelia hermsii KW - Gene polymorphism KW - Nucleotide sequence KW - Relapsing fever KW - Erythrocytes KW - Hydrophobicity KW - Infection KW - Chromosomes KW - Pathogenicity KW - Polymerase chain reaction KW - Repeated DNA sequences KW - Lyme disease KW - Borrelia burgdorferi KW - Borrelia turicatae KW - Spirochetes KW - Open reading frames KW - G 07320:Bacterial genetics KW - J 02740:Genetics and evolution UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17541084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Relapsing+Fever+Spirochetes+Contain+Chromosomal+Genes+with+Unique+Direct+Tandemly+Repeated+Sequences&rft.au=Guyard%2C+Cyril%3BChester%2C+Earl+M%3BRaffel%2C+Sandra+J%3BSchrumpf%2C+Merry+E%3BPolicastro%2C+Paul+F%3BPorcella%2C+Stephen+F%3BLeong%2C+John+M%3BSchwan%2C+Tom+G&rft.aulast=Guyard&rft.aufirst=Cyril&rft.date=2005-05-01&rft.volume=73&rft.issue=5&rft.spage=3025&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Spirochetes; Borrelia burgdorferi; Borrelia turicatae; Borrelia hermsii; Relapsing fever; Nucleotide sequence; Hydrophobicity; Lyme disease; Gene polymorphism; Erythrocytes; Chromosomes; Genomes; Infection; Open reading frames; Pathogenicity; Polymerase chain reaction; Repeated DNA sequences ER - TY - JOUR T1 - Optimization of High-Efficiency Transfection of Adult Human Mesenchymal Stem Cells In Vitro AN - 17508900; 6404639 AB - With the advent of recent protocols to isolate multipotent human mesenchymal stem cells (MSCs), there is a need for efficient transfection methodologies for these cells. Most standard transfection methods yield poor transfection efficiencies for MSCs (<1%). Here we have optimized a high-efficiency transfection technique for low passage MSCs derived from adult human bone marrow. This technique is an extension of electroporation, termed amaxa Nucleofection super(TM), where plasmid DNA is transfected directly into the cell nucleus, independent of the growth state of the cell. With this technique, we demonstrate up to 90% transfection efficiency of the viable population of MSCs, using plasmid construct containing a standard cytomegalovirus (CMV) early promoter driving expression of green fluorescent protein (GFP). Although little variation in transfection efficiency was observed between patient samples, a 2-fold difference in transfection efficiency and a 10-fold difference in expression levels per cell were seen using two distinct CMV-GFP expression plasmids. By fluorescence-activated cell sorting, the GFP expressing cells were sorted and subcultured. At 2 wk posttransfection, approx 25% of the population of sorted cells were GFP positive, and by 3 wk, nearly 10% of the cells still retained GFP expression. Transfection of these cells with plasmid containing either the collagen type I (Col1a1) promoter or the cartilage oligomeric matrix protein (COMP) promoter, each driving expression of GFP, produced a somewhat lower transfection efficiency (approx 40%), due in part to the lower activity of transcription from these promoters compared to that of CMV. Transfection with the collagen type II (Col2a1) promoter linked to GFP exhibited low expression, due to the fact that collagen type II is not expressed in these cells. Upon culturing of the Col2a1-GFP transfected cells in a transforming growth factor- beta 3-containing medium known to induce mesenchymal chondrogensis, a significant enhancement of GFP level was seen, indicating the ability of the transfected cells to differentiate into chondrocytes and express cartilage-specific genes, such as Col2a1. Taken together, these data provide evidence of the applicability of this technique for the efficient transfection of MSCs. JF - Molecular Biotechnology AU - Haleem-Smith, H AU - Derfoul, A AU - Okafor, C AU - Tuli, R AU - Olsen, D AU - Hall, D J AU - Tuan, R S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Drive, Rm 1503, Bethesda, MD 20892-8022, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 009 EP - 020 VL - 30 IS - 1 SN - 1073-6085, 1073-6085 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Bioengineering Abstracts KW - Cartilage oligomeric matrix protein KW - Electroporation KW - Green fluorescent protein KW - Bone marrow KW - Chondrocytes KW - Cytomegalovirus KW - Promoters KW - Stem cells KW - Nuclei KW - Mesenchyme KW - Collagen (type I) KW - Transcription KW - Plasmids KW - Transfection KW - Collagen (type II) KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine KW - W3 33055:Genetic engineering (general) UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17508900?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Biotechnology&rft.atitle=Optimization+of+High-Efficiency+Transfection+of+Adult+Human+Mesenchymal+Stem+Cells+In+Vitro&rft.au=Haleem-Smith%2C+H%3BDerfoul%2C+A%3BOkafor%2C+C%3BTuli%2C+R%3BOlsen%2C+D%3BHall%2C+D+J%3BTuan%2C+R+S&rft.aulast=Haleem-Smith&rft.aufirst=H&rft.date=2005-05-01&rft.volume=30&rft.issue=1&rft.spage=009&rft.isbn=&rft.btitle=&rft.title=Molecular+Biotechnology&rft.issn=10736085&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cytomegalovirus; Transfection; Promoters; Plasmids; Mesenchyme; Stem cells; Collagen (type I); Collagen (type II); Electroporation; Chondrocytes; Cartilage oligomeric matrix protein; Bone marrow; Transcription; Nuclei; Green fluorescent protein ER - TY - JOUR T1 - A Correlation Study of Organochlorine Levels in Serum, Breast Adipose Tissue, and Gluteal Adipose Tissue among Breast Cancer Cases in India AN - 17494031; 6269818 AB - We used data from a breast cancer pilot study carried out in Kerala, India in 1997, for which organochlorine levels were measured in three biological media, blood serum, breast adipose tissue, and gluteal adipose tissue, of 37 fasting breast cancer cases (pretreatment). Our objective was to investigate the relationships between organochlorine concentrations in different biological media. Gas-liquid chromatography determined serum, breast adipose, and gluteal adipose tissue levels of dichlorodiphenyltricholorethane, dichlorodiphenyldichloroethane, beta -benzene hexachloride, and polychlorinated biphenyl (PCB) congeners, PCB-153 and PCB-180. Correlation plots were made and Spearman correlation coefficients (r) calculated for breast adipose tissue versus serum, gluteal adipose tissue versus serum, and breast adipose versus gluteal adipose tissue. We also examined paired ratios of all summary statistics. There were strong correlations among serum, breast adipose tissue, and gluteal adipose tissue concentrations for most organochlorines analyzed, one exception being gluteal versus serum for PCB-153. The correlations for all other comparisons ranged from r = 0.65 to 0.94. Serum (ng/g) versus adipose ratios approached 1:1 for most of the organochlorine pesticide comparisons and did not vary by summary statistic. To our knowledge, this is the first study to use three different media from fasting subjects and to comprehensively investigate the relationship between organochlorines measured across the three media for both organochlorine pesticides and PCBs. These data indicate that blood serum reflects the present body burden of a range of organochlorines to the same extent as adipose tissue, and they support the view that serum may be collected in lieu of adipose tissue to obtain similar information. However, such measurements are a combination of both recent exposures and past exposures, which have metabolized slowly and may still persist. Therefore, investigators should use caution when assigning a level as lifetime body burden. JF - Cancer Epidemiology, Biomarkers & Prevention AU - Rusiecki, Jennifer A AU - Matthews, Aleyama AU - Sturgeon, Susan AU - Sinha, Rashmi AU - Pellizzari, Edo AU - Zheng, Tongzhang AU - Baris, Dalsu AD - Occupational and Environmental Epidemiology and Nutritional Epidemiology Branch, Department of Health and Human Services, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Rockville, Maryland Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1113 EP - 1124 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 14 IS - 5 SN - 1055-9965, 1055-9965 KW - Toxicology Abstracts KW - Organochlorine compounds KW - Chromatography KW - Statistical analysis KW - Pesticides (organochlorine) KW - Fasting KW - biomarkers KW - Blood KW - polychlorinated biphenyls KW - Congeners KW - Breast cancer KW - Adipose tissue KW - PCB KW - X 24133:Metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17494031?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=A+Correlation+Study+of+Organochlorine+Levels+in+Serum%2C+Breast+Adipose+Tissue%2C+and+Gluteal+Adipose+Tissue+among+Breast+Cancer+Cases+in+India&rft.au=Rusiecki%2C+Jennifer+A%3BMatthews%2C+Aleyama%3BSturgeon%2C+Susan%3BSinha%2C+Rashmi%3BPellizzari%2C+Edo%3BZheng%2C+Tongzhang%3BBaris%2C+Dalsu&rft.aulast=Rusiecki&rft.aufirst=Jennifer&rft.date=2005-05-01&rft.volume=14&rft.issue=5&rft.spage=1113&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Adipose tissue; Organochlorine compounds; PCB; Breast cancer; polychlorinated biphenyls; Statistical analysis; Blood; Pesticides (organochlorine); Fasting; Congeners; biomarkers; Chromatography ER - TY - JOUR T1 - Recombinant, Live-Attenuated Tetravalent Dengue Virus Vaccine Formulations Induce a Balanced, Broad, and Protective Neutralizing Antibody Response against Each of the Four Serotypes in Rhesus Monkeys AN - 17492585; 6267490 AB - Three tetravalent vaccine (TV) formulations of previously described monovalent dengue (DEN) virus vaccine candidates were compared to a tetravalent formulation of wild-type DEN viruses (T-wt) for replication in SCID mice transplanted with human liver cells (SCID-HuH-7) or for replication and immunogenicity in rhesus monkeys. TV-1 consists of recombinant DEN1, -2, -3, and -4, each with a 30-nucleotide deletion in the 3' untranslated region ( Delta 30). TV-2 consists of rDEN1 Delta 30, rDEN4 Delta 30, and two antigenic chimeric viruses, rDEN2/4 Delta 30 and rDEN3/4 Delta 30, both also bearing the Delta 30 mutation. TV-3 consists of rDEN1 Delta 30, rDEN2 Delta 30, rDEN4 Delta 30, and a 10-fold higher dose of rDEN3/4 Delta 30. TV-1 and TV-2 were attenuated in SCID-HuH-7 mice with minimal interference in replication among the virus components. TV-1, -2, and -3 were attenuated in rhesus monkeys as measured by duration and peak of viremia. Each monkey immunized with TV-1 and TV-3 seroconverted to the four DEN components by day 28 with neutralization titers ranging from 1:52 to 1:273 and 1:59 to 1:144 for TV-1 and TV-3, respectively. TV-2 induced low antibody titers to DEN2 and DEN3, but a booster immunization after 4 months increased the neutralizing antibody titers to greater than 1:100 against each serotype and elicited broad neutralizing activity against 19 of 20 DEN subtypes. A single dose of TV-2 induced protection against wild-type DEN1, DEN3, and DEN4 challenge, but not DEN2. However, two doses of TV-2 or TV-3 induced protection against DEN2 challenge. Two tetravalent formulations, TV-2 and TV-3, possess properties of a successful DEN vaccine and can be considered for evaluation in clinical trials. JF - Journal of Virology AU - Blaney, Joseph E, Jr AU - Matro, Jennifer M AU - Murphy, Brian R AU - Whitehead, Stephen S AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/05/01/ PY - 2005 DA - 2005 May 01 SP - 5516 EP - 5528 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 79 IS - 9 SN - 0022-538X, 0022-538X KW - Rhesus monkey KW - Rhesus macaque KW - Biotechnology and Bioengineering Abstracts; Immunology Abstracts; Medical and Pharmaceutical Biotechnology Abstracts; Virology & AIDS Abstracts KW - Dengue virus KW - Serotypes KW - Hepatocytes KW - Clinical trials KW - Liver transplantation KW - Gene deletion KW - Allografts KW - Macaca mulatta KW - Deletion KW - Replication KW - Antibody response KW - Immunization KW - Immunogenicity KW - Liver KW - Viremia KW - Vaccines KW - Mutation KW - W3 33365:Vaccines (other) KW - V 22097:Immunization: Vaccines & vaccination: Human KW - F 06100:Vaccines - active immunity KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17492585?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Virology&rft.atitle=Recombinant%2C+Live-Attenuated+Tetravalent+Dengue+Virus+Vaccine+Formulations+Induce+a+Balanced%2C+Broad%2C+and+Protective+Neutralizing+Antibody+Response+against+Each+of+the+Four+Serotypes+in+Rhesus+Monkeys&rft.au=Blaney%2C+Joseph+E%2C+Jr%3BMatro%2C+Jennifer+M%3BMurphy%2C+Brian+R%3BWhitehead%2C+Stephen+S&rft.aulast=Blaney&rft.aufirst=Joseph&rft.date=2005-05-01&rft.volume=79&rft.issue=9&rft.spage=5516&rft.isbn=&rft.btitle=&rft.title=Journal+of+Virology&rft.issn=0022538X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Macaca mulatta; Dengue virus; Vaccines; Replication; Serotypes; Gene deletion; Immunogenicity; Liver transplantation; Viremia; Clinical trials; Liver; Allografts; Hepatocytes; Antibody response; Deletion; Immunization; Mutation ER - TY - JOUR T1 - Cancer Risk Prediction Models: A Workshop on Development, Evaluation, and Application AN - 17491940; 6276440 AB - Cancer researchers, clinicians, and the public are increasingly interested in statistical models designed to predict the occurrence of cancer. As the number and sophistication of cancer risk prediction models have grown, so too has interest in ensuring that they are appropriately applied, correctly developed, and rigorously evaluated. On May 20-21, 2004, the National Cancer Institute sponsored a workshop in which experts identified strengths and limitations of cancer and genetic susceptibility prediction models that were currently in use and under development and explored methodologic issues related to their development, evaluation, and validation. Participants also identified research priorities and resources in the areas of 1) revising existing breast cancer risk assessment models and developing new models, 2) encouraging the development of new risk models, 3) obtaining data to develop more accurate risk models, 4) supporting validation mechanisms and resources, 5) strengthening model development efforts and encouraging coordination, and 6) promoting effective cancer risk communication and decision-making. JF - Journal of the National Cancer Institute AU - Freedman, Andrew N AU - Seminara, Daniela AU - Gail, Mitchell H AU - Hartge, Patricia AU - Colditz, Graham A AU - Ballard-Barbash, Rachel AU - Pfeiffer, Ruth M AD - Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, MD Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 715 EP - 723 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 97 IS - 10 SN - 0027-8874, 0027-8874 KW - Risk Abstracts KW - Prediction KW - Mathematical models KW - Breast cancer KW - Cancer KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17491940?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Cancer+Risk+Prediction+Models%3A+A+Workshop+on+Development%2C+Evaluation%2C+and+Application&rft.au=Freedman%2C+Andrew+N%3BSeminara%2C+Daniela%3BGail%2C+Mitchell+H%3BHartge%2C+Patricia%3BColditz%2C+Graham+A%3BBallard-Barbash%2C+Rachel%3BPfeiffer%2C+Ruth+M&rft.aulast=Freedman&rft.aufirst=Andrew&rft.date=2005-05-01&rft.volume=97&rft.issue=10&rft.spage=715&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-11-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Cancer; Prediction; Mathematical models; Breast cancer ER - TY - JOUR T1 - Bactericidal Activity of First-Choice Antibiotics against Gamma Interferon-Induced Persistent Infection of Human Epithelial Cells by Chlamydia trachomatis AN - 17491853; 6276893 AB - Chlamydia trachomatis is responsible for clinically important chronic inflammatory diseases of humans, including trachoma and pelvic inflammatory disease. Persistent infection of mucosal sites may contribute to the development of these chronic inflammatory diseases. Standard clinical therapy results in satisfactory cure rates of acute infections; however, chronic infection associated with persistence has been suggested to be less responsive to antibiotic therapy. We report the efficiency of two first-line chlamydial antibiotics, azithromycin and doxycycline, under conditions of eradication of C. trachomatis persistent infection using the in vitro model of gamma interferon (IFN- gamma )-mediated persistence and reactivation from persistence. Doxycycline was superior in eradicating acute (minimal bactericidal concentration [MBC] sub(100) = 2.5 to 5.0 mu g/ml) compared to persistent (MBC sub(100) = 10 to 50 mu g/ml) infection. In contrast, azithromycin was significantly more effective in eradicating persistent infection (MBC sub(100) = 2.5 to 5.0 mu g/ml) than acute infection (MBC sub(100) = 10 to 50 mu g/ml). The superior bactericidal effect of azithromycin against persistent infection was found to correlate with the enhanced uptake of the drug by IFN- gamma -treated infected epithelial cells. Based on these findings, we hypothesize that azithromycin should be a particularly efficacious anti-infective agent for the eradication of IFN- gamma -induced chlamydial persistent infection in vivo. JF - Antimicrobial Agents & Chemotherapy AU - Reveneau, Nathalie AU - Crane, Deborah D AU - Fischer, Elizabeth AU - Caldwell, Harlan D AD - Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 1787 EP - 1793 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 49 IS - 5 SN - 0066-4804, 0066-4804 KW - Microbiology Abstracts B: Bacteriology KW - gamma -Interferon KW - Epithelial cells KW - Chlamydia trachomatis KW - Antibiotics KW - Persistent infection KW - Trachoma KW - Antimicrobial agents KW - Inflammatory diseases KW - Azithromycin KW - Chronic infection KW - Pelvic inflammatory disease KW - Bactericidal activity KW - Doxycycline KW - J 02849:Sexually-transmitted diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17491853?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+Agents+%26+Chemotherapy&rft.atitle=Bactericidal+Activity+of+First-Choice+Antibiotics+against+Gamma+Interferon-Induced+Persistent+Infection+of+Human+Epithelial+Cells+by+Chlamydia+trachomatis&rft.au=Reveneau%2C+Nathalie%3BCrane%2C+Deborah+D%3BFischer%2C+Elizabeth%3BCaldwell%2C+Harlan+D&rft.aulast=Reveneau&rft.aufirst=Nathalie&rft.date=2005-05-01&rft.volume=49&rft.issue=5&rft.spage=1787&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+Agents+%26+Chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Epithelial cells; gamma -Interferon; Inflammatory diseases; Azithromycin; Chronic infection; Pelvic inflammatory disease; Antibiotics; Bactericidal activity; Doxycycline; Persistent infection; Antimicrobial agents; Trachoma; Chlamydia trachomatis ER - TY - JOUR T1 - Solution NMR Structure of the 48-kDa IIA super(Mannose)-HPr Complex of the Escherichia coli Mannose Phosphotransferase System AN - 17490333; 6274971 AB - The solution structure of the 48-kDa IIA super(Man)-HPr complex of the mannose branch of the Escherichia coli phosphotransferase system has been solved by NMR using conjoined rigid body/torsion angle-simulated annealing on the basis of intermolecular nuclear Overhauser enhancement data and residual dipolar couplings. IIA super(Man) is dimeric and has two symmetrically related binding sites per dimer for HPr. A convex surface on HPr, formed primarily by helices 1 and 2, interacts with a deep groove at the interface of the two subunits of IIA super(Man). The interaction surface on IIA super(Man) is predominantly helical, comprising helix 3 from the subunit that bears the active site His-10 and helices 1, 4, and 5 from the other subunit. The total buried accessible surface area at the protein-protein interface is 1450 Aa super(2). The binding sites on the two proteins are complementary in terms of shape and distribution of hydrophobic, hydrophilic, and charged residues. The active site histidines, His-10 of IIA super(Man) and His-15 (italics indicate HPr residues) of HPr, are in close proximity. An associative transition state involving a pentacoordinate phosphoryl group with trigonal bipyramidal geometry bonded to the N- epsilon 2 atom of His-10 and the N- delta 1 atom of His-15 can be readily formed with negligible displacement in the backbone coordinates of the residues immediately adjacent to the active site histidines. Comparing the structures of complexes of HPr with three other structurally unrelated phosphotransferase system proteins, enzymes I, IIA super(glucose), and IIA super(mannitol), reveals a number of common features that provide a molecular basis for understanding how HPr specifically recognizes a wide range of diverse proteins. JF - Journal of Biological Chemistry AU - Williams, David CJr AU - Cai, Mengli AU - Suh, Jeong-Yong AU - Peterkofsky, Alan AU - Clore, GMarius AD - Laboratory of Chemical Physics, NIDDK, and the Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 20775 EP - 20784 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 280 IS - 21 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology KW - Mannose KW - Histidine KW - Surface area KW - Escherichia coli KW - N.M.R. KW - Hydrophobicity KW - phosphotransferase KW - J 02728:Enzymes UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17490333?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Solution+NMR+Structure+of+the+48-kDa+IIA+super%28Mannose%29-HPr+Complex+of+the+Escherichia+coli+Mannose+Phosphotransferase+System&rft.au=Williams%2C+David+CJr%3BCai%2C+Mengli%3BSuh%2C+Jeong-Yong%3BPeterkofsky%2C+Alan%3BClore%2C+GMarius&rft.aulast=Williams&rft.aufirst=David&rft.date=2005-05-01&rft.volume=280&rft.issue=21&rft.spage=20775&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Surface area; Histidine; Mannose; Hydrophobicity; N.M.R.; phosphotransferase; Escherichia coli ER - TY - JOUR T1 - Selective depletion strategies in allogeneic stem cell transplantation AN - 17475706; 6654778 AB - Despite improved prophylaxis and treatment, GvHD remains a major limitation to optimal allogeneic stem cell transplantation. Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while useful donor immune function is preserved. The elimination of alloreactive and thereby GvHD-mediating T cells has been shown to be feasible in both pre-clinical and more recently clinical studies. However, SD techniques and the translational research needed for clinical application are still under development. Here we summarize and discuss the following aspects of the SD approach: selection of an appropriate allogeneic stimulator; the responder population; the alloresponse; methods for removal of alloreacting T cells; product testing; clinical considerations. Our review highlights the diversity of possible approaches and the need to develop different techniques for specific clinical applications. JF - Cytotherapy AU - Mielke, S AU - Solomon AU - Barrett, A J AD - Stem Cell Allogeneic Transplantation Section, Hematology Branch, National Heart Lung and Blood Institute, National Institutes of Health (NIH), Bethesda, Maryland, USA Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 109 EP - 115 VL - 7 IS - 2 SN - 1465-3249, 1465-3249 KW - Biotechnology and Bioengineering Abstracts; Medical and Pharmaceutical Biotechnology Abstracts KW - stem cell transplantation KW - Reviews KW - Prophylaxis KW - Lymphocytes T KW - Graft-versus-host reaction KW - W3 33170:Cellular based KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17475706?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cytotherapy&rft.atitle=Selective+depletion+strategies+in+allogeneic+stem+cell+transplantation&rft.au=Mielke%2C+S%3BSolomon%3BBarrett%2C+A+J&rft.aulast=Mielke&rft.aufirst=S&rft.date=2005-05-01&rft.volume=7&rft.issue=2&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=Cytotherapy&rft.issn=14653249&rft_id=info:doi/10.1080%2F14653240510018172 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-04-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Graft-versus-host reaction; Reviews; Lymphocytes T; Prophylaxis; stem cell transplantation DO - http://dx.doi.org/10.1080/14653240510018172 ER - TY - JOUR T1 - Ascorbate Depletion: A Critical Step in Nickel Carcinogenesis? AN - 17425166; 6566815 AB - Nickel compounds are known to cause respiratory cancer in humans and induce tumors in experimental animals. The underlying molecular mechanisms may involve genotoxic effects; however, the data from different research groups are not easy to reconcile. Here, we challenge the common premise that direct genotoxic effects are central to nickel carcinogenesis and probably to that of other metals. Instead, we propose that it is formation of metal complexes with proteins and other molecules that changes cellular homeostasis and provides conditions for selection of cells with transformed phenotype. This is concordant with the major requirement for nickel carcinogenicity, which is prolonged action on the target tissue. If DNA is not the main nickel target, is there another unique molecule that can be attacked with carcinogenic consequences? Our recent observations indicate that ascorbate may be such a molecule. Nickel depletes intracellular ascorbate, which leads to the inhibition of cellular hydroxylases, manifested by the loss of hypoxia-inducible factor (HIF)-1 alpha and -2 alpha hydroxylation and hypoxia-like stress. Proline hydroxylation is crucial for collagen and extracellular matrix assembly as well as for assembly of other protein molecules that have collagen-like domains, including surfactants and complement. Thus, the depletion of ascorbate by chronic exposure to nickel could be deleterious for lung cells and may lead to lung cancer. JF - Environmental Health Perspectives AU - Salnikow, K AU - Kasprzak, K S AD - National Cancer Institute at Frederick, Building 538, Room 205 E, Frederick, MD 21702, USA, salnikow@ncifcrf.gov Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 577 EP - 584 VL - 113 IS - 5 SN - 0091-6765, 0091-6765 KW - Toxicology Abstracts KW - Metals KW - Proline KW - Nickel KW - Genotoxicity KW - Ascorbic acid KW - Collagen KW - Hydroxylation KW - Carcinogenesis KW - DNA KW - Hydroxylase KW - Hypoxia-inducible factors KW - Lung cancer KW - X 24165:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17425166?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Health+Perspectives&rft.atitle=Ascorbate+Depletion%3A+A+Critical+Step+in+Nickel+Carcinogenesis%3F&rft.au=Salnikow%2C+K%3BKasprzak%2C+K+S&rft.aulast=Salnikow&rft.aufirst=K&rft.date=2005-05-01&rft.volume=113&rft.issue=5&rft.spage=577&rft.isbn=&rft.btitle=&rft.title=Environmental+Health+Perspectives&rft.issn=00916765&rft_id=info:doi/10.1289%2Fehp.7605 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-01-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Metals; Proline; Genotoxicity; Carcinogenesis; Nickel; DNA; Hydroxylase; Hypoxia-inducible factors; Lung cancer; Hydroxylation; Collagen; Ascorbic acid DO - http://dx.doi.org/10.1289/ehp.7605 ER - TY - JOUR T1 - Endogenous Kappa -Opioid Receptor Systems Regulate Mesoaccumbal Dopamine Dynamics and Vulnerability to Cocaine AN - 17356317; 6277425 AB - Genetic and pharmacological approaches were used to examine Kappa -opioid receptor (KOR-1) regulation of dopamine (DA) dynamics in the nucleus accumbens and vulnerability to cocaine. Microdialysis revealed that basal DA release and DA extraction fraction (E sub(d)), an indirect measure of DA uptake, are enhanced in KOR-1 knock-out mice. Analysis of DA uptake revealed a decreased K sub(m) but unchanged V sub(max) in knock-outs. Knock-out mice exhibited an augmented locomotor response to cocaine, which did not differ from that of wild-types administered a behavioral sensitizing cocaine treatment. The ability of cocaine to increase DA was enhanced in knock-outs, whereas c-fos induction was decreased. Although repeated cocaine administration to wild types produced behavioral sensitization, knock-outs exhibited no additional enhancement of behavior. Administration of the long-acting KOR antagonist nor-binaltorphimine to wild-type mice increased DA dynamics. However, the effects varied with the duration of KOR-1 blockade. Basal DA release was increased whereas E sub(d) was unaltered after 1 h blockade. After 24 h, release and E sub(d) were increased. The behavioral and neurochemical effects of cocaine were enhanced at both time points. These data demonstrate the existence of an endogenous KOR-1 system that tonically inhibits mesoaccumbal DA neurotransmission. Its loss induces neuroadaptations characteristic of "cocaine-sensitized" animals, indicating a critical role of KOR-1 in attenuating responsiveness to cocaine. The increased DA uptake after pharmacological inactivation or gene deletion highlights the plasticity of mesoaccumbal DA neurons and suggests that loss of KOR-1 and the resultant disinhibition of DA neurons trigger short- and long-term DA transporter adaptations that maintain normal DA levels, despite enhanced release. JF - Journal of Neuroscience AU - Chefer, Vladimir I AU - Czyzyk, Traci AU - Bolan, Elizabeth A AU - Moron, Jose AU - Pintar, John E AU - Shippenberg, Toni S AD - Department of Neuroscience and Cell Biology, Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, and Integrative Neuroscience Section, Behavioral Neurosciences Branch, National Institute on Drug Abuse/Intramural Research Program, Baltimore, Maryland 21224 Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 5029 EP - 5037 PB - Society for Neuroscience, 11 Dupont Circle, N.W. Suite 500 Washington DC 20036 USA, [mailto:info@sfn.org], [URL:http://apu.sfn.org/] VL - 25 IS - 20 SN - 0270-6474, 0270-6474 KW - Opioid receptors (type Kappa ) KW - knockout mice KW - CSA Neurosciences Abstracts; Toxicology Abstracts KW - N3 11106:Neurobiology of drug abuse KW - X 24180:Social poisons & drug abuse UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17356317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Neuroscience&rft.atitle=Endogenous+Kappa+-Opioid+Receptor+Systems+Regulate+Mesoaccumbal+Dopamine+Dynamics+and+Vulnerability+to+Cocaine&rft.au=Chefer%2C+Vladimir+I%3BCzyzyk%2C+Traci%3BBolan%2C+Elizabeth+A%3BMoron%2C+Jose%3BPintar%2C+John+E%3BShippenberg%2C+Toni+S&rft.aulast=Chefer&rft.aufirst=Vladimir&rft.date=2005-05-01&rft.volume=25&rft.issue=20&rft.spage=5029&rft.isbn=&rft.btitle=&rft.title=Journal+of+Neuroscience&rft.issn=02706474&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-03-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Pulsed High-Intensity Focused Ultrasound Enhances Systemic Administration of Naked DNA in Squamous Cell Carcinoma Model: Initial Experience AN - 17337134; 6276669 AB - PURPOSE: To determine whether exposures to pulsed high-intensity focused ultrasound can enhance local delivery and expression of a reporter gene, administered with systemic injection of naked DNA, in tumors in mice. MATERIALS AND METHODS: The study was performed according to an approved animal protocol and in compliance with guidelines of the institutional animal care and use committee. Squamous cell carcinoma (SCC7) tumors were induced subcutaneously in both flanks of female C3H mice (n = 3) and allowed to grow to average size of 0.4 cm super(3). In each mouse, one tumor was exposed to pulsed high-intensity focused ultrasound while a second tumor served as a control. Immediately after ultrasound exposure, a solution containing a cytomegalovirus-green fluorescent protein (GFP) reporter gene construct was injected intravenously via the tail vein. The mouse was sacrificed 24 hours later. Tissue specimens were viewed with fluorescence microscopy to determine the presence of GFP expression, and Western blot analysis was performed, at which signal intensities of expressed GFP were quantitated. A paired Student t test was used to compare mean values in controls with those in treated tumors. Histologic analyses were performed with specific techniques (hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) to determine whether tumor cells had been damaged by ultrasound exposure. RESULTS: GFP expression was present in all sections of tumors that received ultrasound exposure but not in control tumors. Results of signal intensity measurement at Western blot analysis showed expressed GFP to be nine times greater in ultrasound-exposed tumors (160.2 plus or minus 24.5 [standard deviation]) than in controls (17.4 plus or minus 11.8) (P = .004, paired Student t test). Comparison of histologic sections from treated tumors with those from controls revealed no destructive effects from ultrasound exposure. CONCLUSION: Local exposure to pulsed high-intensity focused ultrasound in tumors can enhance the delivery and expression of systemically injected naked DNA. JF - Radiology AU - Dittmar, Kristin M AU - Xie, Jianwu AU - Hunter, Finie AU - Trimble, Cameron AU - Bur, Monica AU - Frenkel, Victor AU - Li, King CP AD - Department of Radiology, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Md Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 541 EP - 546 PB - Radiological Society of North America, 820 Jorie Blvd. Oak Brook Illinois 60523-2251 USA VL - 235 IS - 2 SN - 0033-8419, 0033-8419 KW - Biotechnology and Bioengineering Abstracts; Bioengineering Abstracts KW - W 30965:Miscellaneous, Reviews KW - W4 120:Genetic Engineering in Medicine UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/17337134?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiology&rft.atitle=Pulsed+High-Intensity+Focused+Ultrasound+Enhances+Systemic+Administration+of+Naked+DNA+in+Squamous+Cell+Carcinoma+Model%3A+Initial+Experience&rft.au=Dittmar%2C+Kristin+M%3BXie%2C+Jianwu%3BHunter%2C+Finie%3BTrimble%2C+Cameron%3BBur%2C+Monica%3BFrenkel%2C+Victor%3BLi%2C+King+CP&rft.aulast=Dittmar&rft.aufirst=Kristin&rft.date=2005-05-01&rft.volume=235&rft.issue=2&rft.spage=541&rft.isbn=&rft.btitle=&rft.title=Radiology&rft.issn=00338419&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-08-01 N1 - Last updated - 2011-12-13 ER - TY - JOUR T1 - Application of fuzzy multiple goal programming on the selection for power plants considering site and environmental constraints AN - 16201084; 6434059 AB - A linear programming model with fuzzy multiple goals is presented herein from a cost-effective view to assess the impacts of environmental regulations to restrict carbon emissions on a generation plant. The model contains factors relevant to existing coal power plant scenarios, including clean coal technologies, fuel characteristics, technical alternatives, capital and operation costs, economic and regulatory environment, pipeline issues, and their bounded rationality. Moreover, to achieve the objective of cost minimization, whilst complying with the environmental regulation of carbon emission control in total scale from a long-term perspective, the model explores fuzzy multiple goals based on a power plant scheme. The proposed model was examined via implementations on a real power plant with varying characteristics through fuzzy analysis. Various designed scenarios are analyzed to reveal the option transactions in electricity generation alternatives with pipeline and distance schemes. The results obtained herein provide utility planners with further insight into the systematic analysis and potentials for the cost effectiveness in realistic applications. JF - Journal of Information & Optimization Sciences AU - Tsai, Deng Maw AU - Wang, Earl Juei AD - Department of Industrial Management, National Pingtung University of Science and Technology, 1, Hseuh-Fu Road, Nei-Pu Hsiang, Pingtung (912), Taiwan, R.O.C., dmtsai@npust.edu.tw Y1 - 2005/05// PY - 2005 DA - May 2005 SP - 343 EP - 361 VL - 26 IS - 2 SN - 0252-2667, 0252-2667 KW - Pollution Abstracts KW - Siting criteria KW - Electric power generation KW - Fuels KW - Economics KW - Power plants KW - environmental regulations KW - Emission control KW - Coal KW - P 9000:ENVIRONMENTAL ACTION UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/16201084?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Information+%26+Optimization+Sciences&rft.atitle=Application+of+fuzzy+multiple+goal+programming+on+the+selection+for+power+plants+considering+site+and+environmental+constraints&rft.au=Tsai%2C+Deng+Maw%3BWang%2C+Earl+Juei&rft.aulast=Tsai&rft.aufirst=Deng&rft.date=2005-05-01&rft.volume=26&rft.issue=2&rft.spage=343&rft.isbn=&rft.btitle=&rft.title=Journal+of+Information+%26+Optimization+Sciences&rft.issn=02522667&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2005-09-01 N1 - Last updated - 2015-03-24 N1 - SubjectsTermNotLitGenreText - Siting criteria; Fuels; Electric power generation; Economics; Power plants; environmental regulations; Emission control; Coal ER - TY - JOUR T1 - Plasmodial surface anion channel-independent phloridzin resistance in Plasmodium falciparum. AN - 67779185; 15701633 AB - The plasmodial surface anion channel (PSAC) is an unusual ion channel induced on the human red blood cell membrane after infection with the malaria parasite, Plasmodium falciparum. Because PSAC is permeant to small metabolic precursors essential for parasite growth and is present on red blood cells infected with geographically divergent parasite isolates, it may be an ideal target for future antimalarial development. Here, we used chemically induced mutagenesis and known PSAC antagonists that inhibit in vitro parasite growth to examine whether resistance mutations in PSAC can be readily induced. Stable mutants resistant to phloridzin were generated and selected within 3 weeks after treatment with 1-methyl-3-nitro-1-nitrosoguanidine. These mutants were evaluated with osmotic lysis and electrophysiological transport assays, which indicate that PSAC inhibition by phloridzin is complex with at least two different modes of inhibition. Mutants resistant to the growth inhibitory effects of phloridzin expressed PSAC activity indistinguishable from that on sensitive parasites, indicating selection of resistance via mutations in one or more other parasite targets. Failure to induce mutations in PSAC activity is consistent with a highly constrained channel protein less susceptible to resistance mutations; whether this protein is parasite- or host-encoded remains to be determined. JF - The Journal of biological chemistry AU - Desai, Sanjay A AU - Alkhalil, Abdulnaser AU - Kang, Myungsa AU - Ashfaq, Umar AU - Nguyen, My-Le AD - Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA. sdesai@niaid.nih.gov Y1 - 2005/04/29/ PY - 2005 DA - 2005 Apr 29 SP - 16861 EP - 16867 VL - 280 IS - 17 SN - 0021-9258, 0021-9258 KW - Anions KW - 0 KW - Diuretics KW - Ion Channels KW - Ions KW - Lipid Bilayers KW - Mutagens KW - Nitrosoguanidines KW - surface anion channel protein, Plasmodium falciparum KW - Furosemide KW - 7LXU5N7ZO5 KW - Phlorhizin KW - CU9S17279X KW - Index Medicus KW - Nitrosoguanidines -- pharmacology KW - Animals KW - Dose-Response Relationship, Drug KW - Algorithms KW - Diuretics -- pharmacology KW - Anions -- chemistry KW - Plasmodium falciparum KW - Electrophysiology KW - Protein Binding KW - Models, Biological KW - Osmosis KW - Mutagenesis KW - Kinetics KW - Models, Chemical KW - Furosemide -- pharmacology KW - Allosteric Site KW - Time Factors KW - Mutation KW - Lipid Bilayers -- metabolism KW - Drug Resistance KW - Phlorhizin -- pharmacology KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67779185?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Plasmodial+surface+anion+channel-independent+phloridzin+resistance+in+Plasmodium+falciparum.&rft.au=Desai%2C+Sanjay+A%3BAlkhalil%2C+Abdulnaser%3BKang%2C+Myungsa%3BAshfaq%2C+Umar%3BNguyen%2C+My-Le&rft.aulast=Desai&rft.aufirst=Sanjay&rft.date=2005-04-29&rft.volume=280&rft.issue=17&rft.spage=16861&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural basis for the function of stringent starvation protein a as a transcription factor. AN - 67769285; 15735307 AB - Stringent starvation protein A (SspA) of Escherichia coli is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. We report the crystal structure of Yersinia pestis SspA, which is 83% identical to E. coli SspA in amino acid sequence and is functionally complementary in supporting the lytic growth of phage P1 and acid resistance of an E. coli sspA mutant. The structure reveals that SspA assumes the characteristic fold of glutathione S-transferase (GST). However, SspA lacks GST activity and does not bind glutathione. Three regions of SspA are flexible, the N and C termini and the alpha2-helix. The structure also reveals a conserved surface-exposed pocket composed of residues from a loop between helices alpha3 and alpha4. The functional roles of these structural features were investigated by assessing the ability of deletion and site-directed mutants to confer acid resistance of E. coli and to activate transcription from a phage P1 late promoter, thereby supporting the lytic growth of phage P1. The results indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. The size, shape, and property of the pocket suggest that it mediates protein-protein interactions. SspA orthologs from Y. pestis, Vibrio cholerae, and Pseudomonas aeruginosa are all functional in acid resistance of E. coli, whereas only Y. pestis SspA supports phage P1 growth. JF - The Journal of biological chemistry AU - Hansen, Anne-Marie AU - Gu, Yijun AU - Li, Mi AU - Andrykovitch, Michelle AU - Waugh, David S AU - Jin, Ding Jun AU - Ji, Xinhua AD - Transcription Control Section, Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA. Y1 - 2005/04/29/ PY - 2005 DA - 2005 Apr 29 SP - 17380 EP - 17391 VL - 280 IS - 17 SN - 0021-9258, 0021-9258 KW - Adhesins, Bacterial KW - 0 KW - SspA protein, bacteria KW - Glutathione Transferase KW - EC 2.5.1.18 KW - DNA-Directed RNA Polymerases KW - EC 2.7.7.6 KW - Glutathione KW - GAN16C9B8O KW - Index Medicus KW - Protein Structure, Secondary KW - Stereoisomerism KW - Catalytic Domain KW - Cell Proliferation KW - Mutagenesis, Site-Directed KW - Promoter Regions, Genetic KW - Databases, Protein KW - Genetic Complementation Test KW - Molecular Sequence Data KW - Vibrio cholerae -- metabolism KW - Sequence Homology, Amino Acid KW - Time Factors KW - Protein Conformation KW - Plasmids -- metabolism KW - Models, Molecular KW - Glutathione -- metabolism KW - Dimerization KW - Glutathione Transferase -- metabolism KW - Amino Acid Sequence KW - Gene Deletion KW - Pseudomonas aeruginosa -- metabolism KW - DNA-Directed RNA Polymerases -- metabolism KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Mutation KW - Escherichia coli -- metabolism KW - Adhesins, Bacterial -- metabolism KW - Yersinia pestis -- metabolism KW - Transcription, Genetic UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67769285?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Structural+basis+for+the+function+of+stringent+starvation+protein+a+as+a+transcription+factor.&rft.au=Hansen%2C+Anne-Marie%3BGu%2C+Yijun%3BLi%2C+Mi%3BAndrykovitch%2C+Michelle%3BWaugh%2C+David+S%3BJin%2C+Ding+Jun%3BJi%2C+Xinhua&rft.aulast=Hansen&rft.aufirst=Anne-Marie&rft.date=2005-04-29&rft.volume=280&rft.issue=17&rft.spage=17380&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-21 N1 - Date created - 2005-04-25 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1YY7; PDB N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Expression and distribution of vanilloid receptor 1 (TRPV1) in the adult rat brain. AN - 67784458; 15857679 AB - The vanilloid receptor (TRPV1 or VR1) is a molecular integrator of various painful stimuli, including capsaicin, acid, and high temperature. It can also be activated by endogenous ligands, like the cannabinoid 1 receptor (CB1) agonist anandamide. TRPV1 is well characterized at the terminals of sensory nerves involved in the pain pathway. There is also evidence that TRPV1 is expressed in the brain but little is known about its function. Here, using commercially available specific antibodies to investigate the localization of TRPV1 in the brain of the rat, we report that TRPV1 was expressed in hippocampus, cortex, cerebellum, olfactory bulb, mesencephalon and hindbrain. Immunohistochemical analyses showed high expression in the cell bodies and dendrites of neurons in the hippocampus and in the cortex. To address the question of subcellular localization, immunoelectronmicroscopy was used. TRPV1-like staining was detected in the synapses (mostly, but not exclusively in post-synaptic dendritic spines), on the end feet of astrocytes and in pericytes. In summary, TRPV1 expression shows wide distribution in the brain of the rat, being found in astrocytes and pericytes as well as in neurons. Its localization is consistent with multiple functions within the central nervous system, including the regulation of brain vasculature. JF - Brain research. Molecular brain research AU - Tóth, Attila AU - Boczán, Judit AU - Kedei, Noémi AU - Lizanecz, Erzsébet AU - Bagi, Zsolt AU - Papp, Zoltán AU - Edes, István AU - Csiba, László AU - Blumberg, Peter M AD - Molecular Mechanisms of Tumor Promotion Section, Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. atitoth@jaguar.unideb.hu Y1 - 2005/04/27/ PY - 2005 DA - 2005 Apr 27 SP - 162 EP - 168 VL - 135 IS - 1-2 SN - 0169-328X, 0169-328X KW - Ion Channels KW - 0 KW - TRPV Cation Channels KW - Trpv1 protein, rat KW - Index Medicus KW - Animals KW - Subcellular Fractions -- ultrastructure KW - Astrocytes -- ultrastructure KW - Pericytes -- metabolism KW - Blotting, Western -- methods KW - Immunohistochemistry -- methods KW - Rats KW - Rats, Sprague-Dawley KW - Microscopy, Immunoelectron -- methods KW - Pericytes -- ultrastructure KW - In Vitro Techniques KW - Subcellular Fractions -- metabolism KW - Male KW - Astrocytes -- metabolism KW - Brain -- anatomy & histology KW - Brain -- metabolism KW - Ion Channels -- genetics KW - Ion Channels -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67784458?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Brain+research.+Molecular+brain+research&rft.atitle=Expression+and+distribution+of+vanilloid+receptor+1+%28TRPV1%29+in+the+adult+rat+brain.&rft.au=T%C3%B3th%2C+Attila%3BBocz%C3%A1n%2C+Judit%3BKedei%2C+No%C3%A9mi%3BLizanecz%2C+Erzs%C3%A9bet%3BBagi%2C+Zsolt%3BPapp%2C+Zolt%C3%A1n%3BEdes%2C+Istv%C3%A1n%3BCsiba%2C+L%C3%A1szl%C3%B3%3BBlumberg%2C+Peter+M&rft.aulast=T%C3%B3th&rft.aufirst=Attila&rft.date=2005-04-27&rft.volume=135&rft.issue=1-2&rft.spage=162&rft.isbn=&rft.btitle=&rft.title=Brain+research.+Molecular+brain+research&rft.issn=0169328X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-25 N1 - Date created - 2005-04-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity. AN - 67776812; 15837797 AB - Excessive accumulation of amyloid beta-peptide (Abeta) plays an early and critical role in synapse and neuronal loss in Alzheimer's Disease (AD). Increased oxidative stress is one of the mechanisms whereby Abeta induces neuronal death. Given the lessened susceptibility to oxidative stress exhibited by mice lacking p66Shc, we investigated the role of p66Shc in Abeta toxicity. Treatment of cells and primary neuronal cultures with Abeta caused apoptotic death and induced p66Shc phosphorylation at Ser36. Ectopic expression of a dominant-negative SEK1 mutant or chemical JNK inhibition reduced Abeta-induced JNK activation and p66Shc phosphorylation (Ser36), suggesting that JNK phosphorylates p66Shc. Abeta induced the phosphorylation and hence inactivation of forkhead transcription factors in a p66Shc-dependent manner. Ectopic expression of p66ShcS36A or antioxidant treatment protected cells against Abeta-induced death and reduced forkhead phosphorylation, suggesting that p66Shc phosphorylation critically influences the redox regulation of forkhead proteins and underlies Abeta toxicity. These findings underscore the potential usefulness of JNK, p66Shc, and forkhead proteins as therapeutic targets for AD. JF - The Journal of cell biology AU - Smith, Wanli W AU - Norton, Darrell D AU - Gorospe, Myriam AU - Jiang, Haibing AU - Nemoto, Shino AU - Holbrook, Nikki J AU - Finkel, Toren AU - Kusiak, John W AD - Molecular Neurobiology Unit, Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. wsmith60@jhmi.edu Y1 - 2005/04/25/ PY - 2005 DA - 2005 Apr 25 SP - 331 EP - 339 VL - 169 IS - 2 SN - 0021-9525, 0021-9525 KW - Adaptor Proteins, Signal Transducing KW - 0 KW - Amyloid beta-Peptides KW - Forkhead Transcription Factors KW - Nuclear Proteins KW - SHC1 protein, human KW - Shc Signaling Adaptor Proteins KW - Shc1 protein, mouse KW - Shc1 protein, rat KW - Src Homology 2 Domain-Containing, Transforming Protein 1 KW - Transcription Factors KW - Serine KW - 452VLY9402 KW - JNK Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - MAP Kinase Kinase 4 KW - EC 2.7.12.2 KW - Mitogen-Activated Protein Kinase Kinases KW - Index Medicus KW - Animals KW - Neurons -- metabolism KW - Oxidation-Reduction -- drug effects KW - Alzheimer Disease -- physiopathology KW - Humans KW - Mice KW - Serine -- metabolism KW - Mitogen-Activated Protein Kinase Kinases -- metabolism KW - Phosphorylation -- drug effects KW - Rats KW - Point Mutation KW - Amino Acid Substitution KW - JNK Mitogen-Activated Protein Kinases -- metabolism KW - PC12 Cells KW - Adaptor Proteins, Signal Transducing -- metabolism KW - Transcription Factors -- metabolism KW - Amyloid beta-Peptides -- toxicity KW - Signal Transduction -- drug effects KW - Apoptosis -- drug effects KW - Adaptor Proteins, Signal Transducing -- genetics KW - Nuclear Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67776812?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+cell+biology&rft.atitle=Phosphorylation+of+p66Shc+and+forkhead+proteins+mediates+Abeta+toxicity.&rft.au=Smith%2C+Wanli+W%3BNorton%2C+Darrell+D%3BGorospe%2C+Myriam%3BJiang%2C+Haibing%3BNemoto%2C+Shino%3BHolbrook%2C+Nikki+J%3BFinkel%2C+Toren%3BKusiak%2C+John+W&rft.aulast=Smith&rft.aufirst=Wanli&rft.date=2005-04-25&rft.volume=169&rft.issue=2&rft.spage=331&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+cell+biology&rft.issn=00219525&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-04-26 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Neural Transm Suppl. 2000;59:133-54 [10961426] J Neurosci. 2000 Sep 1;20(17):6442-51 [10964950] Cancer Res. 2000 Sep 15;60(18):5171-8 [11016645] Curr Biol. 2000 Oct 5;10(19):1201-4 [11050388] Trends Biochem Sci. 2002 Jul;27(7):352-60 [12114024] J Neurochem. 2001 Jan;76(2):435-41 [11208906] Brain. 2002 Sep;125(Pt 9):2036-43 [12183349] Neurobiol Aging. 2002 Sep-Oct;23(5):655-64 [12392766] J Biol Chem. 2002 Oct 25;277(43):40302-8 [12192006] Ann N Y Acad Sci. 2004 Mar;1012:153-63 [15105262] Cell. 2004 May 14;117(4):421-6 [15137936] Neurobiol Aging. 2004 May-Jun;25(5):563-8 [15172731] Nature. 2004 Jun 3;429(6991):562-6 [15175753] Science. 2004 Mar 26;303(5666):2011-5 [14976264] J Neurochem. 2004 Jun;89(6):1528-36 [15189356] Science. 2004 Jul 16;305(5682):361 [15192154] Biochim Biophys Acta. 1992 Feb 3;1133(3):275-85 [1737061] Cell. 1992 Jul 10;70(1):93-104 [1623525] Nature. 1992 Dec 17;360(6405):689-92 [1465135] J Biol Chem. 1993 Mar 15;268(8):5748-53 [8449939] J Biol Chem. 1993 Apr 15;268(11):7610-2 [7681824] Nature. 1993 May 6;363(6424):45-51 [8479536] Science. 1993 Jun 25;260(5116):1953-5 [8316835] J Biol Chem. 1993 Oct 15;268(29):21463-5 [7691810] J Biol Chem. 1994 Jan 14;269(2):1143-8 [8288573] Cell. 1994 Jun 17;77(6):817-27 [8004671] Cell. 1994 Sep 23;78(6):949-61 [7923364] Science. 1994 Dec 16;266(5192):1862-5 [7527937] Biochem J. 1995 Oct 1;311 ( Pt 1):1-16 [7575439] J Neurochem. 1995 Oct;65(4):1487-98 [7561842] Nature. 1996 Mar 7;380(6569):75-9 [8598911] Trends Biochem Sci. 1996 Jul;21(7):257-61 [8755247] J Neurosci. 1996 Mar 1;16(5):1710-9 [8774439] EMBO J. 1997 Feb 17;16(4):706-16 [9049300] J Neurosci. 1997 Apr 15;17(8):2653-7 [9092586] Endocrinology. 1997 Jun;138(6):2474-80 [9165038] Neurobiol Dis. 1996 Feb;3(1):3-15 [9173909] Nature. 1997 Oct 30;389(6654):994-9 [9353126] Science. 1997 Nov 14;278(5341):1319-22 [9360933] Mol Cell Biol. 1999 Jan;19(1):751-63 [9858598] Neurobiol Aging. 1998 Sep-Oct;19(5):393-400 [9880041] Cell. 1999 Mar 19;96(6):857-68 [10102273] Nature. 1999 Apr 15;398(6728):630-4 [10217147] J Biol Chem. 1999 Jun 11;274(24):16741-6 [10358014] J Biol Chem. 1999 Jun 11;274(24):17184-92 [10358076] J Neurosci Res. 1999 Jun 1;56(5):457-70 [10369213] Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7421-6 [10377430] Biochemistry. 1999 Jun 15;38(24):7609-16 [10386999] Mol Cell Biol. 2000 Dec;20(23):8969-82 [11073996] Curr Opin Genet Dev. 2000 Dec;10(6):668-74 [11088019] Mol Cell Biol. 2000 Dec;20(24):9138-48 [11094066] Brain Res Mol Brain Res. 2000 Dec 28;85(1-2):221-33 [11146125] Physiol Rev. 2001 Apr;81(2):741-66 [11274343] Nat Genet. 2001 Jun;28(2):139-45 [11381260] Curr Opin Neurobiol. 2001 Jun;11(3):297-305 [11399427] J Biol Chem. 2001 Dec 21;276(51):48332-6 [11602589] Mol Cell Biol. 2002 Apr;22(7):2025-36 [11884591] Science. 2002 Mar 29;295(5564):2450-2 [11884717] J Neurochem. 2002 Mar;80(6):1049-61 [11953455] Science. 2002 Apr 19;296(5567):530-4 [11964479] J Biol Chem. 2002 May 17;277(20):17649-56 [11882652] Oncogene. 2002 May 30;21(24):3872-8 [12032825] J Cell Mol Med. 2001 Jan-Mar;5(1):1-17 [12067447] J Biol Chem. 2003 Jan 31;278(5):3437-45 [12435748] Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11836-41 [10518537] Nature. 1999 Nov 18;402(6759):309-13 [10580504] J Biol Chem. 1999 Dec 24;274(52):37111-6 [10601271] Nature. 2000 Apr 13;404(6779):782-7 [10783894] IUBMB Life. 2000 Mar;49(3):177-80 [10868907] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure. AN - 67753758; 15701627 AB - Mouse fibroblasts, deficient in DNA polymerase beta, are hypersensitive to monofunctional DNA methylating agents such as methyl methanesulfonate (MMS). Both wild-type and, in particular, repair-deficient DNA polymerase beta null cells are highly sensitized to the cytotoxic effects of MMS by 4-amino-1,8-naphthalimide (4-AN), an inhibitor of poly(ADP-ribose) polymerase (PARP) activity. Experiments with synchronized cells suggest that exposure during S-phase of the cell cycle is required for the 4-AN effect. 4-AN elicits a similar extreme sensitization to the thymidine analog, 5-hydroxymethyl-2'-deoxyuridine, implicating the requirement for an intermediate of DNA repair. In PARP-1-expressing fibroblasts treated with a combination of MMS and 4-AN, a complete inhibition of DNA synthesis is apparent after 4 h, and by 24 h, all cells are arrested in S-phase of the cell cycle. Continuous incubation with 4-AN is required to maintain the cell cycle arrest. Caffeine, an inhibitor of the upstream checkpoint kinases ATM (ataxia telangiectasia-mutated) and ATR (ATM and Rad3-related), has no effect on the early inhibition of DNA synthesis, but cells are no longer able to maintain the block after 8 h. Instead, the addition of caffeine leads to arrest of cells in G(2)/M rather than S-phase after 24 h. Analysis of signaling pathways in cell extracts reveals an activation of Chk1 after treatment with MMS and 4-AN, which can be suppressed by caffeine. Our results suggest that inhibition of PARP activity results in sensitization to MMS through maintenance of an ATR and Chk1-dependent S-phase checkpoint. JF - The Journal of biological chemistry AU - Horton, Julie K AU - Stefanick, Donna F AU - Naron, Jana M AU - Kedar, Padmini S AU - Wilson, Samuel H AD - Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. Y1 - 2005/04/22/ PY - 2005 DA - 2005 Apr 22 SP - 15773 EP - 15785 VL - 280 IS - 16 SN - 0021-9258, 0021-9258 KW - Antineoplastic Agents, Alkylating KW - 0 KW - Naphthalimides KW - Poly(ADP-ribose) Polymerase Inhibitors KW - Quinolones KW - 4-amino-1,8-naphthalimide KW - 1742-95-6 KW - DNA KW - 9007-49-2 KW - 1-Naphthylamine KW - 9753I242R5 KW - Methyl Methanesulfonate KW - AT5C31J09G KW - Poly(ADP-ribose) Polymerases KW - EC 2.4.2.30 KW - Index Medicus KW - Fibroblasts -- drug effects KW - Animals KW - DNA Methylation KW - Antineoplastic Agents, Alkylating -- pharmacology KW - Mice KW - Fibroblasts -- metabolism KW - Quinolones -- pharmacology KW - Methyl Methanesulfonate -- pharmacology KW - DNA Damage -- drug effects KW - Signal Transduction -- physiology KW - Poly(ADP-ribose) Polymerases -- genetics KW - 1-Naphthylamine -- analogs & derivatives KW - DNA -- metabolism KW - Cell Cycle -- physiology KW - Poly(ADP-ribose) Polymerases -- metabolism KW - 1-Naphthylamine -- pharmacology KW - Cell Cycle -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67753758?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+biological+chemistry&rft.atitle=Poly%28ADP-ribose%29+polymerase+activity+prevents+signaling+pathways+for+cell+cycle+arrest+after+DNA+methylating+agent+exposure.&rft.au=Horton%2C+Julie+K%3BStefanick%2C+Donna+F%3BNaron%2C+Jana+M%3BKedar%2C+Padmini+S%3BWilson%2C+Samuel+H&rft.aulast=Horton&rft.aufirst=Julie&rft.date=2005-04-22&rft.volume=280&rft.issue=16&rft.spage=15773&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+biological+chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-06-23 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase II clinical trial of ixabepilone (BMS-247550), an epothilone B analog, in metastatic and locally advanced breast cancer. AN - 67757381; 15837987 AB - Ixabepilone (BMS-247550) is an epothilone B analog that stabilizes microtubules and has antitumor activity in taxane-refractory patients in phase I studies. In a phase II trial, we evaluated the efficacy and safety of ixabepilone in women with metastatic and locally advanced breast cancer. Breast cancer patients with measurable disease who had paclitaxel and/or docetaxel as prior neoadjuvant, adjuvant, or metastatic therapy were treated with ixabepilone at 6 mg/m2/d intravenously on days 1 through 5 every 3 weeks. Levels of glutamate (glu) -terminated and acetylated alpha-tubulin, markers of microtubule stabilization, were detected by Western blot and by immunohistochemistry in a subset of matched pre- and post-treatment tumor biopsies. Thirty-seven patients received 153 cycles of ixabepilone. The best responses were a complete response in one patient (3%), partial responses in seven patients (19%), and stable disease in 13 patients (35%). Grade 3 and 4 toxicities included neutropenia (35%), febrile neutropenia (14%), fatigue (14%), diarrhea (11%), nausea/vomiting (5%), myalgia/arthralgia (3%), and sensory neuropathy (3%). Two patients were removed from study because of prolonged grade 2 or 3 neurotoxicity, and three patients were removed from study for other grade 3 and 4 nonhematologic toxicities. Compared with baseline levels, levels of both glu-terminated and acetylated alpha-tubulin were increased in tumor biopsies performed after ixabepilone therapy. An objective response was seen in 22% of the patients in a population who had been previously treated with a taxane. Sensory neuropathy was mild with grade 3 neurotoxicity rarely seen. Microtubule stabilization occurred in tumor biopsies after treatment with ixabepilone. JF - Journal of clinical oncology : official journal of the American Society of Clinical Oncology AU - Low, Jennifer A AU - Wedam, Suparna B AU - Lee, James J AU - Berman, Arlene W AU - Brufsky, Adam AU - Yang, Sherry X AU - Poruchynsky, Marianne S AU - Steinberg, Seth M AU - Mannan, Nitin AU - Fojo, Tito AU - Swain, Sandra M AD - Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20889-5015, USA. Y1 - 2005/04/20/ PY - 2005 DA - 2005 Apr 20 SP - 2726 EP - 2734 VL - 23 IS - 12 SN - 0732-183X, 0732-183X KW - Antineoplastic Agents, Phytogenic KW - 0 KW - Epothilones KW - Taxoids KW - docetaxel KW - 15H5577CQD KW - ixabepilone KW - K27005NP0A KW - Paclitaxel KW - P88XT4IS4D KW - Index Medicus KW - Infusions, Intravenous KW - Humans KW - Antineoplastic Agents, Phytogenic -- therapeutic use KW - Nervous System -- drug effects KW - Adult KW - Treatment Outcome KW - Paclitaxel -- therapeutic use KW - Middle Aged KW - Taxoids -- therapeutic use KW - Nervous System -- pathology KW - Immunohistochemistry KW - Female KW - Breast Neoplasms -- drug therapy KW - Epothilones -- therapeutic use KW - Breast Neoplasms -- pathology KW - Epothilones -- adverse effects KW - Epothilones -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67757381?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.atitle=Phase+II+clinical+trial+of+ixabepilone+%28BMS-247550%29%2C+an+epothilone+B+analog%2C+in+metastatic+and+locally+advanced+breast+cancer.&rft.au=Low%2C+Jennifer+A%3BWedam%2C+Suparna+B%3BLee%2C+James+J%3BBerman%2C+Arlene+W%3BBrufsky%2C+Adam%3BYang%2C+Sherry+X%3BPoruchynsky%2C+Marianne+S%3BSteinberg%2C+Seth+M%3BMannan%2C+Nitin%3BFojo%2C+Tito%3BSwain%2C+Sandra+M&rft.aulast=Low&rft.aufirst=Jennifer&rft.date=2005-04-20&rft.volume=23&rft.issue=12&rft.spage=2726&rft.isbn=&rft.btitle=&rft.title=Journal+of+clinical+oncology+%3A+official+journal+of+the+American+Society+of+Clinical+Oncology&rft.issn=0732183X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-19 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase. AN - 67751565; 15837423 AB - Inositol hexakisphosphate and other inositol high polyphosphates have diverse and critical roles in eukaryotic regulatory pathways. Inositol 1,3,4-trisphosphate 5/6-kinase catalyzes the rate-limiting step in inositol high polyphosphate synthesis in animals. This multifunctional enzyme also has inositol 3,4,5,6-tetrakisphosphate 1-kinase and other activities. The structure of an archetypal family member, from Entamoeba histolytica, has been determined to 1.2 A resolution in binary and ternary complexes with nucleotide, substrate, and product. The structure reveals an ATP-grasp fold. The inositol ring faces ATP edge-on such that the 5- and 6-hydroxyl groups are nearly equidistant from the ATP gamma-phosphate in catalytically productive phosphoacceptor positions and explains the unusual dual site specificity of this kinase. Inositol tris- and tetrakisphosphates interact via three phosphate binding subsites and one solvent-exposed site that could in principle be occupied by 18 different substrates, explaining the mechanisms for the multiple specificities and catalytic activities of this enzyme. JF - Molecular cell AU - Miller, Gregory J AU - Wilson, Monita P AU - Majerus, Philip W AU - Hurley, James H AD - Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, United States Department of Health and Human Services, Bethesda, Maryland 20892, USA. Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 201 EP - 212 VL - 18 IS - 2 SN - 1097-2765, 1097-2765 KW - Inositol Phosphates KW - 0 KW - Ligands KW - Adenosine Diphosphate KW - 61D2G4IYVH KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - inositol 1,3,4-trisphosphate KW - 98102-63-7 KW - Phosphotransferases (Alcohol Group Acceptor) KW - EC 2.7.1.- KW - myo-inositol-trisphosphate 6-kinase KW - EC 2.7.1.134 KW - Magnesium KW - I38ZP9992A KW - Index Medicus KW - Molecular Structure KW - Animals KW - Stereoisomerism KW - Protein Structure, Secondary KW - Electrons KW - Models, Molecular KW - Inositol Phosphates -- metabolism KW - DNA Mutational Analysis KW - Humans KW - Amino Acid Sequence KW - Entamoeba histolytica -- chemistry KW - Protein Binding KW - Binding Sites KW - Mutagenesis, Site-Directed KW - Magnesium -- metabolism KW - Adenosine Triphosphate -- metabolism KW - Molecular Sequence Data KW - Substrate Specificity KW - Molecular Conformation KW - Sequence Homology, Amino Acid KW - Protein Structure, Tertiary KW - Spodoptera -- cytology KW - Adenosine Diphosphate -- metabolism KW - Phosphotransferases (Alcohol Group Acceptor) -- chemistry KW - Phosphotransferases (Alcohol Group Acceptor) -- biosynthesis KW - Crystallography, X-Ray KW - Phosphotransferases (Alcohol Group Acceptor) -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67751565?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cell&rft.atitle=Specificity+determinants+in+inositol+polyphosphate+synthesis%3A+crystal+structure+of+inositol+1%2C3%2C4-trisphosphate+5%2F6-kinase.&rft.au=Miller%2C+Gregory+J%3BWilson%2C+Monita+P%3BMajerus%2C+Philip+W%3BHurley%2C+James+H&rft.aulast=Miller&rft.aufirst=Gregory&rft.date=2005-04-15&rft.volume=18&rft.issue=2&rft.spage=201&rft.isbn=&rft.btitle=&rft.title=Molecular+cell&rft.issn=10972765&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-05-31 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1Z2P; PDB; 1Z2O; 1Z2N N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Colorectal papillomavirus infection in patients with colorectal cancer. AN - 67749292; 15837733 AB - Infection with human papillomaviruses (HPV) is associated with the development of cervical cancer, but whether HPVs have a role in colorectal cancer remains controversial. To determine the relationship between HPV and colorectal cancer, we did a retrospective, controlled study using tumor and tumor-adjacent colorectal tissues dissected from patients with colorectal cancer, as well as colorectal tissues from control individuals with no cancer. The samples were processed in a blinded fashion for nested PCR and in situ PCR detection of HPV DNAs. The PCR products were gel-purified and sequenced for HPV genotyping. We found that colorectal tissues from 28 of 55 (51%) patients with colorectal cancer were positive for HPV DNA. Colorectal tissues from all 10 control individuals were negative for HPV DNA (P = 0.0034). Of the 107 usable (GAPDH(+)) samples collected as paired colorectal tissues (tumor and tumor-adjacent tissues) from the patients, 38 (36%) had HPV16 (n = 31), HPV18 (n = 5), or HPV45 (n = 2), with HPV DNA in both tumor and tumor-adjacent tissues of 10 paired samples, 13 in only the tumor, and 5 in only tumor-adjacent tissues. In situ PCR detection of the tumor tissues confirmed the presence of HPV DNA in tumor cells. Our results suggest that colorectal HPV infection is common in patients with colorectal cancer, albeit at a low DNA copy number, with HPV16 being the most prevalent type. HPV infection may play a role in colorectal carcinogenesis. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Bodaghi, Sohrab AU - Yamanegi, Koji AU - Xiao, Shu-Yuan AU - Da Costa, Maria AU - Palefsky, Joel M AU - Zheng, Zhi-Ming AD - HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 2862 EP - 2867 VL - 11 IS - 8 SN - 1078-0432, 1078-0432 KW - DNA, Viral KW - 0 KW - Index Medicus KW - Rectum -- virology KW - Colon -- virology KW - Reproducibility of Results KW - Colon -- pathology KW - Humans KW - Aged KW - Carcinoma, Squamous Cell -- virology KW - Adenocarcinoma -- pathology KW - Rectum -- pathology KW - Aged, 80 and over KW - Carcinoma, Squamous Cell -- pathology KW - Polymerase Chain Reaction -- methods KW - Adult KW - Middle Aged KW - DNA, Viral -- isolation & purification KW - Adenocarcinoma -- virology KW - DNA, Viral -- genetics KW - Male KW - Female KW - Papillomavirus Infections -- pathology KW - Colorectal Neoplasms -- pathology KW - Papillomavirus Infections -- virology KW - Colorectal Neoplasms -- ethnology KW - Papillomaviridae -- genetics KW - Colorectal Neoplasms -- virology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67749292?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Colorectal+papillomavirus+infection+in+patients+with+colorectal+cancer.&rft.au=Bodaghi%2C+Sohrab%3BYamanegi%2C+Koji%3BXiao%2C+Shu-Yuan%3BDa+Costa%2C+Maria%3BPalefsky%2C+Joel+M%3BZheng%2C+Zhi-Ming&rft.aulast=Bodaghi&rft.aufirst=Sohrab&rft.date=2005-04-15&rft.volume=11&rft.issue=8&rft.spage=2862&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-08-29 N1 - Date created - 2005-04-19 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Natl Cancer Inst Monogr. 2003;(31):52-6 [12807946] Cancer Res. 2003 Nov 1;63(21):7515-9 [14612553] Arch Surg. 1990 Jul;125(7):862-5 [2164371] Science. 1991 Jun 21;252(5013):1643-51 [2047872] Am J Surg Pathol. 1992 Mar;16(3):269-75 [1317997] Gastroenterology. 2001 Mar;120(4):988-94 [11231953] J Natl Cancer Inst. 2000 May 3;92(9):709-20 [10793107] Cancer Res. 1999 Dec 15;59(24):6132-6 [10626803] J Clin Microbiol. 2000 Jan;38(1):357-61 [10618116] Eur J Cancer Prev. 1998 Aug;7(4):305-13 [9806119] N Engl J Med. 1997 Nov 6;337(19):1350-8 [9358129] J Gen Virol. 1995 Apr;76 ( Pt 4):1057-62 [9049358] Gut. 1995 Jul;37(1):87-90 [7672688] J Clin Microbiol. 1995 Apr;33(4):901-5 [7790457] J Surg Oncol. 1992 Sep;51(1):5-7 [1325576] Am J Surg. 1993 Dec;166(6):738-40; discussion 741-2 [8273860] Int J Cancer. 2003 Apr 10;104(3):336-44 [12569557] CA Cancer J Clin. 2003 Jan-Feb;53(1):5-26 [12568441] Clin Cancer Res. 2002 Oct;8(10):3187-92 [12374687] J Clin Pathol. 2002 Oct;55(10):721-8 [12354793] Int J Colorectal Dis. 2002 Nov;17(6):396-401 [12355215] J Med Virol. 2002 Nov;68(3):412-6 [12226830] Nat Rev Cancer. 2002 May;2(5):342-50 [12044010] J Infect Dis. 2002 May 1;185(9):1229-37 [12001039] J Clin Virol. 2001 May;21(2):129-34 [11378493] J Microbiol Immunol Infect. 2001 Jun;34(2):87-91 [11456365] Pathologica. 2001 Oct;93(5):531-4 [11725354] Cancer Res. 2001 Apr 1;61(7):2799-803 [11306446] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Supplementary analysis of probabilities at the termination of a group sequential phase II trial. AN - 67523574; 15565737 AB - We consider estimation of various probabilities after termination of a group sequential phase II trial. A motivating example is that the stopping rule of a phase II oncologic trial is determined solely based on response to a drug treatment, and at the end of the trial estimating the rate of toxicity and response is desirable. The conventional maximum likelihood estimator (sample proportion) of a probability is shown to be biased, and two alternative estimators are proposed to correct for bias, a bias-reduced estimator obtained by using Whitehead's bias-adjusted approach, and an unbiased estimator from the Rao-Blackwell method of conditioning. All three estimation procedures are shown to have certain invariance property in bias. Moreover, estimators of a probability and their bias and precision can be evaluated through the observed response rate and the stage at which the trial stops, thus avoiding extensive computation. Copyright 2004 John Wiley & Sons, Ltd. JF - Statistics in medicine AU - Liu, Aiyi AU - Wu, Chengqing AU - Yu, Kai F AU - Gehan, Edmund AD - Biometry and Mathematical Statistics Branch, Department of Health and Human Services, National Institute of Child Health and Human Development, 6100 Executive Boulevard, Rockville, MD 20892, USA. liua@mail.nih.gov Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - 1009 EP - 1027 VL - 24 IS - 7 SN - 0277-6715, 0277-6715 KW - Index Medicus KW - Computer Simulation KW - Humans KW - Drug-Related Side Effects and Adverse Reactions KW - Bias (Epidemiology) KW - Data Interpretation, Statistical KW - Clinical Trials, Phase III as Topic -- methods KW - Models, Biological UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67523574?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Statistics+in+medicine&rft.atitle=Supplementary+analysis+of+probabilities+at+the+termination+of+a+group+sequential+phase+II+trial.&rft.au=Liu%2C+Aiyi%3BWu%2C+Chengqing%3BYu%2C+Kai+F%3BGehan%2C+Edmund&rft.aulast=Liu&rft.aufirst=Aiyi&rft.date=2005-04-15&rft.volume=24&rft.issue=7&rft.spage=1009&rft.isbn=&rft.btitle=&rft.title=Statistics+in+medicine&rft.issn=02776715&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2005-07-05 N1 - Date created - 2005-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CONF T1 - Hepatitis C virus infection and substance abuse: medical management and developing models of integrated care--an introduction. AN - 67515709; 15768332 JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Kresina, Thomas F AU - Khalsa, Jag AU - Cesari, Helen AU - Francis, Henry Y1 - 2005/04/15/ PY - 2005 DA - 2005 Apr 15 SP - S259 EP - S262 VL - 40 Suppl 5 KW - Index Medicus KW - Models, Organizational KW - Risk Factors KW - Mental Disorders -- epidemiology KW - Humans KW - Delivery of Health Care -- classification KW - Treatment Outcome KW - Mental Disorders -- complications KW - Health Services Accessibility KW - Comorbidity KW - Hepatitis C -- therapy KW - HIV Infections -- complications KW - Delivery of Health Care, Integrated -- organization & administration KW - Hepatitis C -- complications KW - HIV Infections -- therapy KW - Substance Abuse, Intravenous -- therapy KW - Substance Abuse, Intravenous -- epidemiology KW - Hepatitis C -- epidemiology KW - Substance Abuse, Intravenous -- complications KW - HIV Infections -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67515709?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Hepatitis+C+virus+infection+and+substance+abuse%3A+medical+management+and+developing+models+of+integrated+care--an+introduction.&rft.au=Kresina%2C+Thomas+F%3BKhalsa%2C+Jag%3BCesari%2C+Helen%3BFrancis%2C+Henry&rft.aulast=Kresina&rft.aufirst=Thomas&rft.date=2005-04-15&rft.volume=40+Suppl+5&rft.issue=&rft.spage=S259&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-10-06 N1 - Date created - 2005-03-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Daily versus As-Needed Corticosteroids for Mild Persistent Asthma AN - 223931590; 15829533 AB - Background Although guidelines recommend daily therapy for patients with mild persistent asthma, prescription patterns suggest that most such patients use these so-called controller therapies intermittently. In patients with mild persistent asthma, we evaluated the efficacy of intermittent short-course corticosteroid treatment guided by a symptom-based action plan alone or in addition to daily treatment with either inhaled budesonide or oral zafirlukast over a one-year period. Methods In a double-blind trial, 225 adults underwent randomization. The primary outcome was morning peak expiratory flow (PEF). Other outcomes included the forced expiratory volume in one second (FEV1 ) before and after bronchodilator treatment, the frequency of exacerbations, the degree of asthma control, the number of symptom-free days, and the quality of life. Results The three treatments produced similar increases in morning PEF (7.1 to 8.3 percent; approximately 32 liters per minute; P=0.90) and similar rates of asthma exacerbations (P=0.24), even though the intermittent-treatment group took budesonide, on average, for only 0.5 week of the year. As compared with intermittent therapy or daily zafirlukast therapy, daily budesonide therapy produced greater improvements in pre-bronchodilator FEV1 (P=0.005), bronchial reactivity (P<0.001), the percentage of eosinophils in sputum (P=0.007), exhaled nitric oxide levels (P=0.006), scores for asthma control (P<0.001), and the number of symptom-free days (P=0.03), but not in post-bronchodilator FEV1 (P=0.29) or in the quality of life (P=0.18). Daily zafirlukast therapy did not differ significantly from intermittent treatment in any outcome measured. Conclusions It may be possible to treat mild persistent asthma with short, intermittent courses of inhaled or oral corticosteroids taken when symptoms worsen. Further studies are required to determine whether this novel approach to treatment should be recommended. JF - The New England Journal of Medicine AU - Boushey, Homer A, MD AU - Sorkness, Christine A, PharmD AU - King, Tonya S, PhD AU - Sullivan, Sean D, PhD AU - Fahy, John V, MD AU - Lazarus, Stephen C, MD AU - Chinchilli, Vernon M, PhD AU - Craig, Timothy J, DO AU - Dimango, Emily A, MD AU - Deykin, Aaron, MD AU - Fagan, Joanne K, PhD AU - Fish, James E, MD AU - Ford, Jean G, MD AU - Kraft, Monica, MD AU - Lemanske, Robert F, Jr, MD AU - Leone, Frank T, MD AU - Martin, Richard J, MD AU - Mauger, Elizabeth A, PhD AU - Pesola, Gene R, MD, MPH AU - Peters, Stephen P, MD, PhD AU - Rollings, Nancy J, MEd AU - Szefler, Stanley J, MD AU - Wechsler, Michael E, MD AU - Israel, Elliot, MD Y1 - 2005/04/14/ PY - 2005 DA - 2005 Apr 14 SP - 1519 EP - 28 CY - Boston PB - Massachusetts Medical Society VL - 352 IS - 15 SN - 00284793 KW - Medical Sciences KW - Adrenal Cortex Hormones KW - Anti-Asthmatic Agents KW - Bronchodilator Agents KW - Tosyl Compounds KW - zafirlukast KW - Budesonide KW - Asthma KW - Airway management KW - Administration, Oral KW - Asthma -- classification KW - Drug Administration Schedule KW - Double-Blind Method KW - Bronchodilator Agents -- administration & dosage KW - Humans KW - Peak Expiratory Flow Rate -- drug effects KW - Asthma -- physiopathology KW - Adult KW - Administration, Inhalation KW - Female KW - Forced Expiratory Volume -- drug effects KW - Male KW - Asthma -- drug therapy KW - Anti-Asthmatic Agents -- administration & dosage KW - Adrenal Cortex Hormones -- administration & dosage KW - Budesonide -- administration & dosage KW - Tosyl Compounds -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/223931590?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthcompleteshell&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+Journal+of+Medicine&rft.atitle=Daily+versus+As-Needed+Corticosteroids+for+Mild+Persistent+Asthma&rft.au=Boushey%2C+Homer+A%2C+MD%3BSorkness%2C+Christine+A%2C+PharmD%3BKing%2C+Tonya+S%2C+PhD%3BSullivan%2C+Sean+D%2C+PhD%3BFahy%2C+John+V%2C+MD%3BLazarus%2C+Stephen+C%2C+MD%3BChinchilli%2C+Vernon+M%2C+PhD%3BCraig%2C+Timothy+J%2C+DO%3BDimango%2C+Emily+A%2C+MD%3BDeykin%2C+Aaron%2C+MD%3BFagan%2C+Joanne+K%2C+PhD%3BFish%2C+James+E%2C+MD%3BFord%2C+Jean+G%2C+MD%3BKraft%2C+Monica%2C+MD%3BLemanske%2C+Robert+F%2C+Jr%2C+MD%3BLeone%2C+Frank+T%2C+MD%3BMartin%2C+Richard+J%2C+MD%3BMauger%2C+Elizabeth+A%2C+PhD%3BPesola%2C+Gene+R%2C+MD%2C+MPH%3BPeters%2C+Stephen+P%2C+MD%2C+PhD%3BRollings%2C+Nancy+J%2C+MEd%3BSzefler%2C+Stanley+J%2C+MD%3BWechsler%2C+Michael+E%2C+MD%3BIsrael%2C+Elliot%2C+MD&rft.aulast=Boushey&rft.aufirst=Homer&rft.date=2005-04-14&rft.volume=352&rft.issue=15&rft.spage=1519&rft.isbn=&rft.btitle=&rft.title=The+New+England+Journal+of+Medicine&rft.issn=00284793&rft_id=info:doi/ LA - English DB - ProQuest Central N1 - Copyright - Copyright © 2005 Massachusetts Medical Society. All rights reserved. N1 - Document feature - Graphs; Tables; References N1 - Last updated - 2014-04-29 N1 - CODEN - NEJMAG ER - TY - JOUR T1 - Activation of orbital and medial prefrontal cortex by methylphenidate in cocaine-addicted subjects but not in controls: relevance to addiction. AN - 67743194; 15829645 AB - Drugs of abuse are rewarding to addicted and nonaddicted subjects, but they trigger craving and compulsive intake only in addicted subjects. Here, we used positron emission tomography (PET) and [18F] deoxyglucose to compare the brain metabolic responses (marker of brain function) of cocaine-addicted subjects (n = 21) and controls (n = 15) to identify brain regions that are uniquely activated in addicted subjects by intravenous methylphenidate (a drug that cocaine-addicted subjects report to be similar to cocaine). In parallel, we also measured the changes in dopamine (DA) induced by intravenous methylphenidate (using PET and [11C] raclopride) in the striatum and in the thalamus. Metabolic responses between groups differed significantly only in the right medial orbital prefrontal cortex [Brodmann's area (BA) 25 and medial BA 11], where methylphenidate increased metabolism in addicted subjects but decreased metabolism in controls. These changes were associated in all subjects with increased "desire for methylphenidate" and in the addicted subjects with "cocaine craving." In addicted subjects, increases in BA 25 were also associated with mood elevation. Methylphenidate-induced increases in metabolism in the medial orbital prefrontal cortex were associated with its increase of DA in the thalamus but not in the striatum. These findings provide evidence that enhanced sensitivity of BA 25 (region involved with emotional reactivity) and BA 11 (region involved with salience attribution and motivation) in cocaine-addicted subjects may underlie the strong emotional response to the drug and the intense desire to procure it that results in craving and compulsive drug intake. It also suggests that the mesothalamic DA pathway may contribute to these processes. JF - The Journal of neuroscience : the official journal of the Society for Neuroscience AU - Volkow, Nora D AU - Wang, Gene-Jack AU - Ma, Yeming AU - Fowler, Joanna S AU - Wong, Christopher AU - Ding, Yu-Shin AU - Hitzemann, Robert AU - Swanson, James M AU - Kalivas, Peter AD - National Institute of Drug Abuse, Rockville, Maryland 20857, USA. nvolkow@nida.nih.gov Y1 - 2005/04/13/ PY - 2005 DA - 2005 Apr 13 SP - 3932 EP - 3939 VL - 25 IS - 15 KW - Carbon Isotopes KW - 0 KW - Central Nervous System Stimulants KW - Dopamine Antagonists KW - Fluorodeoxyglucose F18 KW - 0Z5B2CJX4D KW - Methylphenidate KW - 207ZZ9QZ49 KW - Raclopride KW - 430K3SOZ7G KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Brain Mapping KW - Fluorodeoxyglucose F18 -- pharmacokinetics KW - Positron-Emission Tomography -- methods KW - Humans KW - Adult KW - Carbon Isotopes -- pharmacokinetics KW - Dopamine -- metabolism KW - Dopamine Antagonists -- pharmacokinetics KW - Statistics as Topic KW - Raclopride -- pharmacokinetics KW - Male KW - Functional Laterality KW - Prefrontal Cortex -- diagnostic imaging KW - Methylphenidate -- administration & dosage KW - Behavior, Addictive -- chemically induced KW - Cocaine-Related Disorders -- psychology KW - Behavior, Addictive -- metabolism KW - Central Nervous System Stimulants -- administration & dosage KW - Prefrontal Cortex -- drug effects KW - Cocaine-Related Disorders -- metabolism KW - Behavior, Addictive -- diagnostic imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/67743194?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.atitle=Activation+of+orbital+and+medial+prefrontal+cortex+by+methylphenidate+in+cocaine-addicted+subjects+but+not+in+controls%3A+relevance+to+addiction.&rft.au=Volkow%2C+Nora+D%3BWang%2C+Gene-Jack%3BMa%2C+Yeming%3BFowler%2C+Joanna+S%3BWong%2C+Christopher%3BDing%2C+Yu-Shin%3BHitzemann%2C+Robert%3BSwanson%2C+James+M%3BKalivas%2C+Peter&rft.aulast=Volkow&rft.aufirst=Nora&rft.date=2005-04-13&rft.volume=25&rft.issue=15&rft.spage=3932&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+neuroscience+%3A+the+official+journal+of+the+Society+for+Neuroscience&rft.issn=1529-2401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2006-02-13 N1 - Date created - 2005-04-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER -