TY - JOUR T1 - Proliferation and apoptosis in PhIP-induced rat mammary gland carcinomas with elevated phosphotyrosine-STAT5a. AN - 68375782; 17173897 AB - In the present study we addressed whether proliferation and apoptosis in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced rat mammary gland carcinomas were different between carcinomas with high and low expression of phosphotyrosine (pY)-STAT5a. We determined that carcinomas with high pY-STAT5a were more proliferative (MIB5 immunostaining) and had a higher expression of cyclin D1 and estrogen receptor alpha. Furthermore, carcinomas with elevated pY-STAT5a demonstrated lower apoptosis as measured by the TUNEL assay and the Bcl-2 to Bax ratio, and showed increased expression of the long and short isoforms of the prolactin receptor. The results of this study are consistent with the notion that activated STAT5a may provide a growth advantage in some types of mammary gland cancers. JF - FEBS letters AU - Papaconstantinou, Andriana D AU - Snyderwine, Elizabeth G AD - Chemical Carcinogenesis Section, Laboratory of Experimental Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892, USA. Y1 - 2007/01/09/ PY - 2007 DA - 2007 Jan 09 SP - 29 EP - 33 VL - 581 IS - 1 SN - 0014-5793, 0014-5793 KW - Bax protein, rat KW - 0 KW - Carcinogens KW - Cyclin D KW - Cyclins KW - Estrogen Receptor alpha KW - Imidazoles KW - STAT5 Transcription Factor KW - Stat5a protein, rat KW - bcl-2-Associated X Protein KW - Phosphotyrosine KW - 21820-51-9 KW - 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine KW - 909C6UN66T KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Tumor Cells, Cultured KW - Mammary Glands, Animal -- metabolism KW - Mammary Glands, Animal -- pathology KW - Cyclins -- metabolism KW - Estrogen Receptor alpha -- metabolism KW - Female KW - bcl-2-Associated X Protein -- metabolism KW - STAT5 Transcription Factor -- metabolism KW - Mammary Neoplasms, Experimental -- chemically induced KW - Imidazoles -- toxicity KW - Apoptosis KW - Phosphotyrosine -- metabolism KW - Carcinogens -- toxicity KW - Mammary Neoplasms, Experimental -- metabolism KW - Cell Proliferation KW - Mammary Neoplasms, Experimental -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68375782?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=FEBS+letters&rft.atitle=Proliferation+and+apoptosis+in+PhIP-induced+rat+mammary+gland+carcinomas+with+elevated+phosphotyrosine-STAT5a.&rft.au=Papaconstantinou%2C+Andriana+D%3BSnyderwine%2C+Elizabeth+G&rft.aulast=Papaconstantinou&rft.aufirst=Andriana&rft.date=2007-01-09&rft.volume=581&rft.issue=1&rft.spage=29&rft.isbn=&rft.btitle=&rft.title=FEBS+letters&rft.issn=00145793&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-27 N1 - Date created - 2006-12-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - TLR Ligands Influence the Quality of T Cell Responses T2 - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AN - 39312707; 4502251 JF - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AU - Seder, Robert A Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - Lymphocytes T KW - Ligands KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39312707?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.atitle=TLR+Ligands+Influence+the+Quality+of+T+Cell+Responses&rft.au=Seder%2C+Robert+A&rft.aulast=Seder&rft.aufirst=Robert&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=85 5&AllowFutureView=1 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Human Visceral Leishmaniasis is Associated with IL-10 Producing T Cells that are Distinct from CD4+CD25+ (Foxp3) Regulatory T Cells T2 - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AN - 39306975; 4502276 JF - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AU - Nylen-Spoomaker, Susanne I S Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - Lymphocytes T KW - Interleukin 10 KW - Immunoregulation KW - Foxp3 protein KW - CD25 antigen KW - CD4 antigen KW - Visceral leishmaniasis KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39306975?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.atitle=Human+Visceral+Leishmaniasis+is+Associated+with+IL-10+Producing+T+Cells+that+are+Distinct+from+CD4%2BCD25%2B+%28Foxp3%29+Regulatory+T+Cells&rft.au=Nylen-Spoomaker%2C+Susanne+I+S&rft.aulast=Nylen-Spoomaker&rft.aufirst=Susanne+I&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=85 5&AllowFutureView=1 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Gene Expression Signatures of the Immune Response to Guide the Diagnosis, Prognosis and Treatment of Cancer T2 - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AN - 39303383; 4502263 JF - 2007 Keystone Symposia on Immunological Intervention in Human Disease (A2) AU - Staudt, Louis M Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - Cancer KW - Immune response KW - Gene expression KW - Prognosis KW - Immunity KW - Defense mechanisms KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39303383?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.atitle=Gene+Expression+Signatures+of+the+Immune+Response+to+Guide+the+Diagnosis%2C+Prognosis+and+Treatment+of+Cancer&rft.au=Staudt%2C+Louis+M&rft.aulast=Staudt&rft.aufirst=Louis&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2007+Keystone+Symposia+on+Immunological+Intervention+in+Human+Disease+%28A2%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=85 5&AllowFutureView=1 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Detection and Visualization of Low-Population Encounter Complexes in Protein-Protein and Protein-DNA Association by Paramagnetic Relaxation Enhancement T2 - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AN - 39255213; 4506215 JF - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AU - Clore, G Marius Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - Nucleic acids KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39255213?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.atitle=Detection+and+Visualization+of+Low-Population+Encounter+Complexes+in+Protein-Protein+and+Protein-DNA+Association+by+Paramagnetic+Relaxation+Enhancement&rft.au=Clore%2C+G+Marius&rft.aulast=Clore&rft.aufirst=G&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=86 8 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - NMR Assignment of Polymerasebeta Labeled with 2H, 13C, and 15N in Complex with Substrate DNA T2 - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AN - 39249059; 4506195 JF - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AU - Mueller, Geoffrey A Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - N.M.R. KW - Nitrogen isotopes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39249059?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.atitle=NMR+Assignment+of+Polymerasebeta+Labeled+with+2H%2C+13C%2C+and+15N+in+Complex+with+Substrate+DNA&rft.au=Mueller%2C+Geoffrey+A&rft.aulast=Mueller&rft.aufirst=Geoffrey&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=86 8 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Weak Alignment Offers New Opportunities in NMR of Biomolecules T2 - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AN - 39248963; 4506186 JF - X Keystone Symposia on Frontiers of NMR in Molecular Biology (A3) AU - Bax, Ad Y1 - 2007/01/06/ PY - 2007 DA - 2007 Jan 06 KW - N.M.R. KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39248963?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.atitle=Weak+Alignment+Offers+New+Opportunities+in+NMR+of+Biomolecules&rft.au=Bax%2C+Ad&rft.aulast=Bax&rft.aufirst=Ad&rft.date=2007-01-06&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=X+Keystone+Symposia+on+Frontiers+of+NMR+in+Molecular+Biology+%28A3%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=86 8 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Dual dopamine/serotonin releasers as potential medications for stimulant and alcohol addictions. AN - 70347813; 17408232 AB - We have advocated the idea of agonist therapy for treating cocaine addiction. This strategy involves administration of stimulant-like medications (eg, monoamine releasers) to alleviate withdrawal symptoms and prevent relapse. A major limitation of this approach is that many candidate medicines possess significant abuse potential because of activation of mesolimbic dopamine (DA) neurons in central nervous system reward circuits. Previous data suggest that serotonin (5-HT) neurons can provide an inhibitory influence over mesolimbic DA neurons. Thus, it might be predicted that the balance between DA and 5-HT transmission is important to consider when developing medications with reduced stimulant side effects. In this article, we discuss several issues related to the development of dual DA/5-HT releasers for the treatment of substance use disorders. First, we discuss evidence supporting the existence of a dual deficit in DA and 5-HT function during withdrawal from chronic cocaine or alcohol abuse. Then we summarize studies that have tested the hypothesis that 5-HT neurons can dampen the effects mediated by mesolimbic DA. For example, it has been shown that pharmacological manipulations that increase extracellular 5-HT attenuate stimulant effects produced by DA release, such as locomotor stimulation and self-administration behavior. Finally, we discuss our recently published data about PAL-287 (naphthylisopropylamine), a novel non-amphetamine DA-/5-HT-releasing agent that suppresses cocaine self-administration but lacks positive reinforcing properties. It is concluded that DA/5-HT releasers might be useful therapeutic adjuncts for the treatment of cocaine and alcohol addiction, obesity, and even attention deficit disorder and depression. JF - The AAPS journal AU - Rothman, Richard B AU - Blough, Bruce E AU - Baumann, Michael H AD - Clinical Psychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Department of Health and Human Services, Baltimore, MD 21224, USA. rrothman@mail.nih.gov Y1 - 2007/01/05/ PY - 2007 DA - 2007 Jan 05 SP - E1 EP - 10 VL - 9 IS - 1 KW - Dopamine Agents KW - 0 KW - Serotonin Agents KW - Serotonin KW - 333DO1RDJY KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Molecular Structure KW - Animals KW - Substance Withdrawal Syndrome -- metabolism KW - Substance Withdrawal Syndrome -- prevention & control KW - Humans KW - Structure-Activity Relationship KW - Serotonin Agents -- chemistry KW - Dopamine Agents -- therapeutic use KW - Dopamine -- deficiency KW - Serotonin Agents -- pharmacology KW - Dopamine Agents -- pharmacology KW - Cocaine-Related Disorders -- drug therapy KW - Dopamine -- metabolism KW - Alcoholism -- metabolism KW - Serotonin Agents -- therapeutic use KW - Serotonin -- metabolism KW - Dopamine Agents -- chemistry KW - Cocaine-Related Disorders -- metabolism KW - Alcoholism -- drug therapy KW - Serotonin -- deficiency UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70347813?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+AAPS+journal&rft.atitle=Dual+dopamine%2Fserotonin+releasers+as+potential+medications+for+stimulant+and+alcohol+addictions.&rft.au=Rothman%2C+Richard+B%3BBlough%2C+Bruce+E%3BBaumann%2C+Michael+H&rft.aulast=Rothman&rft.aufirst=Richard&rft.date=2007-01-05&rft.volume=9&rft.issue=1&rft.spage=E1&rft.isbn=&rft.btitle=&rft.title=The+AAPS+journal&rft.issn=1550-7416&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-05-03 N1 - Date created - 2007-04-05 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Prog Neurobiol. 2005 Apr;75(6):406-33 [15955613] Physiology (Bethesda). 2005 Aug;20:225-31 [16024510] Am J Psychiatry. 2005 Aug;162(8):1414-22 [16055762] Eur J Pharmacol. 2005 Dec 5;526(1-3):113-24 [16288736] Synapse. 2006 Apr;59(5):277-89 [16416445] Neural Netw. 2002 Jun-Jul;15(4-6):603-16 [12371515] Alcohol Clin Exp Res. 2003 Feb;27(2):232-43 [12605072] Front Neuroendocrinol. 1994 Jun;15(2):85-156 [7813744] J Exp Biol. 1994 Nov;196:229-36 [7823024] J Exp Biol. 1994 Nov;196:263-81 [7823027] N Engl J Med. 1995 Nov 2;333(18):1196-203 [7565976] J Pharmacol Exp Ther. 1995 Sep;274(3):1182-91 [7562486] MMWR Morb Mortal Wkly Rep. 1995 Dec 1;44(47):882-6 [7476843] J Pharmacol Exp Ther. 1995 Dec;275(3):1551-9 [8531128] J Neurosci. 1996 May 15;16(10):3474-85 [8627380] Clin Neuropharmacol. 1995 Apr;18(2):183-95 [8635177] Curr Probl Cardiol. 1999 Dec;24(12):745-92 [10609092] Mol Pharmacol. 2000 Jan;57(1):75-81 [10617681] Synapse. 2000 May;36(2):102-13 [10767057] J Subst Abuse Treat. 2000 Jul;19(1):77-9 [10867304] Psychopharmacology (Berl). 2000 Jul;150(4):361-73 [10958077] Synapse. 2001 Jan;39(1):32-41 [11071707] Circulation. 2000 Dec 5;102(23):2836-41 [11104741] Psychol Addict Behav. 2000 Dec;14(4):390-6 [11130157] Neuropsychopharmacology. 2001 May;24(5):492-501 [11282249] Am J Med Sci. 2001 Apr;321(4):292-9 [11307870] J Clin Psychopharmacol. 2001 Oct;21(5):522-6 [11593078] Pharmacol Biochem Behav. 2002 Jan-Feb;71(1-2):197-204 [11812523] J Pharmacol Exp Ther. 2002 Mar;300(3):831-7 [11861788] Pharmacol Biochem Behav. 2002 Apr;71(4):825-36 [11888573] Ann N Y Acad Sci. 2002 Jun;965:109-26 [12105089] Pharmacol Ther. 2002 Jul;95(1):73-88 [12163129] Nat Med. 2002 Oct;8(10):1129-35 [12244304] Alcohol. 1997 Jan-Feb;14(1):45-8 [9014023] Mol Psychiatry. 1996 Jul;1(3):232-54 [9118348] Neuroreport. 1997 Apr 14;8(6):1347-51 [9172133] Adv Pharmacol. 1998;42:995-7 [9328065] J Clin Psychopharmacol. 1997 Dec;17(6):485-8 [9408812] Am J Addict. 1998 Spring;7(2):142-55 [9598218] Ann N Y Acad Sci. 1998 May 30;844:59-74 [9668665] Synapse. 1998 Sep;30(1):107-11 [9704887] Drug Alcohol Depend. 1998 Jun-Jul;51(1-2):87-96 [9716932] Biol Psychiatry. 1998 Oct 1;44(7):578-91 [9787882] J Pharmacol Exp Ther. 1999 Feb;288(2):550-60 [9918558] Psychopharmacology (Berl). 1999 Apr;143(3):309-14 [10353435] Alcohol. 1999 May;18(1):55-64 [10386666] Psychopharmacology (Berl). 1999 Jun;144(4):389-97 [10435412] Pharmacol Rev. 1999 Sep;51(3):439-64 [10471414] Psychopharmacology (Berl). 1999 Aug;145(3):283-94 [10494577] Drug Alcohol Depend. 2003 May 1;70(1):39-52 [12681524] Drug Alcohol Depend. 2003 May 1;70(1):101-4 [12681530] Psychopharmacology (Berl). 2003 May;167(3):324-32 [12652348] Mol Pharmacol. 2003 Jun;63(6):1223-9 [12761331] Psychopharmacology (Berl). 2003 Jul;168(1-2):146-54 [12529808] Nat Rev Neurosci. 2003 Oct;4(10):819-28 [14523381] Eur J Pharmacol. 2003 Oct 31;479(1-3):23-40 [14612135] Eur J Pharmacol. 2003 Oct 31;479(1-3):229-36 [14612153] Eur J Pharmacol. 2003 Nov 7;480(1-3):151-62 [14623358] Neuropsychopharmacology. 2004 Apr;29(4):660-8 [14627998] Neuropsychopharmacology. 2004 May;29(5):969-81 [15039761] Addict Behav. 2004 Sep;29(7):1439-64 [15345275] Cor Vasa. 1985;27(2-3):160-71 [3928246] Neurosci Biobehav Rev. 1985 Fall;9(3):469-77 [2999657] Arch Gen Psychiatry. 1986 Feb;43(2):107-13 [3947206] Pharmacol Biochem Behav. 1986 Oct;25(4):849-55 [2431419] J Med Chem. 1990 Feb;33(2):703-10 [1967651] Pharmacol Biochem Behav. 1990 Jan;35(1):237-44 [2315363] Neuropsychopharmacology. 1991 Jan;4(1):17-26 [2003866] Ciba Found Symp. 1992;166:7-14; discussion 14-9 [1638922] Pharmacol Biochem Behav. 1993 Mar;44(3):651-5 [8451268] Annu Rev Neurosci. 1993;16:73-93 [8096377] Md Med J. 1993 Feb;42(2):153-6 [8097012] J Clin Pharmacol. 1993 Apr;33(4):296-310 [8473543] Biochim Biophys Acta. 1993 Oct 4;1144(3):249-63 [8104483] J Subst Abuse Treat. 1994 May-Jun;11(3):273-5 [8072057] J Psychoactive Drugs. 1994 Apr-Jun;26(2):119-28 [7931856] Nat Rev Neurosci. 2004 Dec;5(12):963-70 [15550951] Synapse. 2005 May;56(2):94-9 [15729739] J Pharmacol Exp Ther. 2005 May;313(2):848-54 [15677348] J Pharmacol Exp Ther. 2005 Jun;313(3):1361-9 [15761112] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Drugs and valvular heart disease. AN - 68392191; 17202450 JF - The New England journal of medicine AU - Roth, Bryan L AD - Department of Pharmacology, Schools of Medicine and Pharmacy, National Institute of Mental Health Psychoactive Drug Screening Program at the University of North Carolina, Chapel Hill, USA. Y1 - 2007/01/04/ PY - 2007 DA - 2007 Jan 04 SP - 6 EP - 9 VL - 356 IS - 1 KW - Antiparkinson Agents KW - 0 KW - Dopamine Agonists KW - Ergolines KW - Receptor, Serotonin, 5-HT2B KW - Serotonin 5-HT2 Receptor Agonists KW - Serotonin Receptor Agonists KW - Pergolide KW - 24MJ822NZ9 KW - cabergoline KW - LL60K9J05T KW - Abridged Index Medicus KW - Index Medicus KW - Serotonin Receptor Agonists -- pharmacology KW - Antiparkinson Agents -- adverse effects KW - Serotonin Receptor Agonists -- adverse effects KW - Receptor, Serotonin, 5-HT2B -- metabolism KW - Humans KW - Pergolide -- adverse effects KW - Dopamine Agonists -- adverse effects KW - Heart Valve Diseases -- chemically induced KW - Ergolines -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68392191?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+New+England+journal+of+medicine&rft.atitle=Drugs+and+valvular+heart+disease.&rft.au=Roth%2C+Bryan+L&rft.aulast=Roth&rft.aufirst=Bryan&rft.date=2007-01-04&rft.volume=356&rft.issue=1&rft.spage=6&rft.isbn=&rft.btitle=&rft.title=The+New+England+journal+of+medicine&rft.issn=1533-4406&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-09 N1 - Date created - 2007-01-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: N Engl J Med. 2007 Apr 19;356(16):1677-8; author reply 1678-80 [17447276] N Engl J Med. 2007 Apr 19;356(16):1677; author reply 1678-80 [17447278] N Engl J Med. 2007 Apr 19;356(16):1677; author reply 1678-80 [17447277] Comment On: N Engl J Med. 2007 Jan 4;356(1):39-46 [17202454] N Engl J Med. 2007 Jan 4;356(1):29-38 [17202453] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Report of the Working Group on Integrated Translational Research in DNA Repair AN - 20287677; 7272808 AB - On September 28-29, 2006, the National Institute of Environmental Health Sciences led a team from the National Institutes of Health in hosting a Working Group on Integrated Translational Research in DNA Repair, in Berkeley, CA. In recognition of the far-reaching goals for this area of investigation, the Working Group was charged with conceiving a vision to facilitate projects that would apply the lessons of DNA Repair research to clinical application and public health. The participants included basic and physician scientists working in the various areas of DNA Repair and genome stability, as well as agency representatives of the National Cancer Institute and the National Institute of General Medical Sciences. In constructing this vision of practical research recommendations, the Working Group was asked to identify roadblocks to progress, suggest enabling technologies, and to consider areas that are ripe for translation. This report summarizes the rationale for this initiative and the recommendations that emerged. JF - DNA Repair AU - Reinlib, L AU - Friedberg, E C AD - National Institute of Environmental Health Sciences, National Institutes of Health, U.S. Department of Health and Human Services, Research Triangle Park, NC, USA, Reinlib@niehs.nih.gov Y1 - 2007/01/04/ PY - 2007 DA - 2007 Jan 04 SP - 145 EP - 147 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 6 IS - 1 SN - 1568-7864, 1568-7864 KW - Health & Safety Science Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Genomes KW - Translation KW - Vision KW - DNA KW - Environmental health KW - DNA repair KW - Cancer KW - Technology KW - Public health KW - N 14820:DNA Metabolism & Structure KW - H 12000:Epidemiology and Public Health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20287677?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=DNA+Repair&rft.atitle=Report+of+the+Working+Group+on+Integrated+Translational+Research+in+DNA+Repair&rft.au=Reinlib%2C+L%3BFriedberg%2C+E+C&rft.aulast=Reinlib&rft.aufirst=L&rft.date=2007-01-04&rft.volume=6&rft.issue=1&rft.spage=145&rft.isbn=&rft.btitle=&rft.title=DNA+Repair&rft.issn=15687864&rft_id=info:doi/10.1016%2Fj.dnarep.2006.11.006 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Genomes; Translation; Vision; DNA repair; Cancer; Public health; DNA; Environmental health; Technology DO - http://dx.doi.org/10.1016/j.dnarep.2006.11.006 ER - TY - JOUR T1 - Human MutL alpha : The jack of all trades in MMR is also an endonuclease AN - 19777499; 7272806 AB - Recently, Paul Modrich's group reported the discovery of an intrinsic endonuclease activity for human MutL alpha . This breakthrough provides a satisfactory answer to the longstanding puzzle of a missing nuclease activity in human mismatch repair and will undoubtedly lead to new investigations of DNA repair and replication. Here, the implications of this exciting new finding are discussed in the context of mismatch repair in Escherichia coli and humans. JF - DNA Repair AU - Yang, W AD - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA, Wei.Yang@nih.gov Y1 - 2007/01/04/ PY - 2007 DA - 2007 Jan 04 SP - 135 EP - 139 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 6 IS - 1 SN - 1568-7864, 1568-7864 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - mismatch repair KW - Replication KW - Escherichia coli KW - Nuclease KW - Endonuclease KW - DNA repair KW - N 14820:DNA Metabolism & Structure KW - J 02400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19777499?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=DNA+Repair&rft.atitle=Human+MutL+alpha+%3A+The+jack+of+all+trades+in+MMR+is+also+an+endonuclease&rft.au=Yang%2C+W&rft.aulast=Yang&rft.aufirst=W&rft.date=2007-01-04&rft.volume=6&rft.issue=1&rft.spage=135&rft.isbn=&rft.btitle=&rft.title=DNA+Repair&rft.issn=15687864&rft_id=info:doi/10.1016%2Fj.dnarep.2006.10.021 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - mismatch repair; Replication; Nuclease; DNA repair; Endonuclease; Escherichia coli DO - http://dx.doi.org/10.1016/j.dnarep.2006.10.021 ER - TY - JOUR T1 - Risk of soft tissue sarcomas by individual subtype in survivors of hereditary retinoblastoma. AN - 68394384; 17202110 AB - Survivors of hereditary retinoblastoma have an increased risk for second malignancies, especially soft tissue sarcomas. However, the risks of individual histologic subtypes of soft tissue sarcomas have not been evaluated. We estimated the risk for six subtypes of soft tissue sarcomas (fibrosarcoma, liposarcoma, histiocytoma, leiomyosarcoma, rhabdomyosarcoma, and others) in a cohort of 963 one-year survivors of hereditary retinoblastoma among patients diagnosed at two US institutions from 1914 through 1984. We calculated standardized incidence ratios (SIRs) for specific subtypes of soft tissue sarcomas by comparison with population data from the Connecticut Tumor Registry or from National Cancer Institute Surveillance, Epidemiology, and End Results database. We also calculated the cumulative risk for all soft tissue sarcomas combined. We observed 69 soft tissue sarcomas in 68 patients with hereditary retinoblastoma. Risks were elevated for soft tissue sarcomas overall (SIR = 184, 95% confidence interval [CI] = 143 to 233) and for individual subtypes. Leiomyosarcoma was the most frequent subtype (SIR = 390, 95% CI = 247 to 585), with 78% of leiomyosarcomas diagnosed 30 or more years after the retinoblastoma diagnosis (SIR = 435, 95% CI = 258 to 687). Among patients treated with radiotherapy for retinoblastoma, we found statistically significantly increased risks of soft tissue sarcomas in the field of radiation. Irradiated patients also had increased risks of soft tissue sarcomas, especially leiomyosarcomas, outside the field of radiation, and risks of soft tissue sarcomas were increased in nonirradiated patients as well, indicating a genetic predisposition to soft tissue sarcomas independent of radiation. The cumulative risk for any soft tissue sarcoma 50 years after radiotherapy for retinoblastoma was 13.1% (95% CI = 9.7% to 17.0%). Long-term follow-up of a cohort of survivors of hereditary retinoblastoma revealed a statistically significant excess of leiomyosarcoma and other soft tissue sarcomas that persists decades after the retinoblastoma diagnosis. Retinoblastoma survivors should undergo regular medical surveillance for sarcomas in their adult years. JF - Journal of the National Cancer Institute AU - Kleinerman, Ruth A AU - Tucker, Margaret A AU - Abramson, David H AU - Seddon, Johanna M AU - Tarone, Robert E AU - Fraumeni, Joseph F AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, EPS 7044, 6120 Executive Blvd., Rockville, MD 20852, USA. kleinerr@mail.nih.gov Y1 - 2007/01/03/ PY - 2007 DA - 2007 Jan 03 SP - 24 EP - 31 VL - 99 IS - 1 KW - Index Medicus KW - Odds Ratio KW - Humans KW - SEER Program KW - Aged KW - Child KW - Radiotherapy, Adjuvant -- adverse effects KW - Risk Assessment KW - Radiotherapy -- adverse effects KW - Child, Preschool KW - Registries KW - Infant KW - Leiomyosarcoma -- etiology KW - Retinal Neoplasms -- genetics KW - Adult KW - Confounding Factors (Epidemiology) KW - Incidence KW - Middle Aged KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Leiomyosarcoma -- epidemiology KW - United States -- epidemiology KW - Male KW - Chemotherapy, Adjuvant KW - Female KW - Survivors -- statistics & numerical data KW - Neoplasms, Second Primary -- epidemiology KW - Neoplasms, Second Primary -- etiology KW - Retinoblastoma -- genetics KW - Neoplasms, Second Primary -- genetics KW - Sarcoma -- genetics KW - Retinoblastoma -- radiotherapy KW - Sarcoma -- etiology KW - Retinoblastoma -- drug therapy KW - Sarcoma -- epidemiology KW - Population Surveillance UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68394384?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Risk+of+soft+tissue+sarcomas+by+individual+subtype+in+survivors+of+hereditary+retinoblastoma.&rft.au=Kleinerman%2C+Ruth+A%3BTucker%2C+Margaret+A%3BAbramson%2C+David+H%3BSeddon%2C+Johanna+M%3BTarone%2C+Robert+E%3BFraumeni%2C+Joseph+F&rft.aulast=Kleinerman&rft.aufirst=Ruth&rft.date=2007-01-03&rft.volume=99&rft.issue=1&rft.spage=24&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=1460-2105&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-07 N1 - Date created - 2007-01-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Comment In: J Natl Cancer Inst. 2007 Jan 3;99(1):3-5 [17202103] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - A randomized clinical trial of coenzyme Q10 and GPI-1485 in early Parkinson disease. AN - 68390093; 17200487 AB - To determine if future studies of coenzyme Q(10) and GPI-1485 in Parkinson disease (PD) may be warranted. We conducted a randomized, double-blind, calibrated futility clinical trial of coenzyme Q10 and GPI-1485 in early untreated PD using placebo data from the DATATOP study to establish the futility threshold. The primary outcome measure (change in total Unified Parkinson's Disease Rating Scale scores over 1 year) did not meet the prespecified criteria for futility for either agent. Secondary analyses using calibration controls and other more recent placebo data question the appropriateness of the predetermined definition of futility, and suggest that a more restrictive threshold may be needed. Coenzyme Q(10) and GPI-1485 may warrant further study in Parkinson disease, although the data are inconsistent. Additional factors (cost, availability of other agents, more recent data on placebo outcomes, other ongoing trials) should also be considered in the selection of agents for Phase III studies. JF - Neurology AU - NINDS NET-PD Investigators AD - NINDS NET-PD Investigators Y1 - 2007/01/02/ PY - 2007 DA - 2007 Jan 02 SP - 20 EP - 28 VL - 68 IS - 1 KW - Coenzymes KW - 0 KW - GPI 1485 KW - Ubiquinone KW - 1339-63-5 KW - coenzyme Q10 KW - EJ27X76M46 KW - Tacrolimus KW - WM0HAQ4WNM KW - Abridged Index Medicus KW - Index Medicus KW - Nausea -- chemically induced KW - Headache -- chemically induced KW - Double-Blind Method KW - Humans KW - Aged KW - Middle Aged KW - Male KW - Female KW - Tacrolimus -- adverse effects KW - Tacrolimus -- therapeutic use KW - Ubiquinone -- therapeutic use KW - Tacrolimus -- analogs & derivatives KW - Ubiquinone -- adverse effects KW - Parkinson Disease -- physiopathology KW - Parkinson Disease -- enzymology KW - Ubiquinone -- analogs & derivatives KW - Parkinson Disease -- drug therapy KW - Parkinson Disease -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68390093?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurology&rft.atitle=A+randomized+clinical+trial+of+coenzyme+Q10+and+GPI-1485+in+early+Parkinson+disease.&rft.au=NINDS+NET-PD+Investigators&rft.aulast=NINDS+NET-PD+Investigators&rft.aufirst=&rft.date=2007-01-02&rft.volume=68&rft.issue=1&rft.spage=20&rft.isbn=&rft.btitle=&rft.title=Neurology&rft.issn=1526-632X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-05 N1 - Date created - 2007-01-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Long-lasting and transmission-blocking activity of antibodies to Plasmodium falciparum elicited in mice by protein conjugates of Pfs25 AN - 19843979; 7254905 AB - Malaria is a leading cause of morbidity and mortality, estimated to cause >1 million childhood deaths annually. Plasmodium falciparum causes the most severe form of the disease. There is as yet no licensed vaccine for this disease, despite over a half century of research. In this study, we investigated a transmission-blocking vaccine candidate, the ookinete surface protein Pfs25. Antibodies against Pfs25, drawn in during a bite, can block parasite development in the mosquito midgut, preventing transmission to other individuals. Pfs25 is a low-molecular-weight protein, by itself not immunogenic. To increase its immunogenicity, we investigated several methods of conjugating Pfs25 to itself and to other proteins: recombinant Pseudomonas aeruginosa exotoxin A, and ovalbumin, using amide, hydrazone, or thioether linkages. All conjugates were immunogenic and induced booster responses in mice. The scheme to form amide bonds between proteins by using adipic acid dihydrizide as a linker produced the most immunogenic conjugates. Adsorption of the conjugates onto aluminum hydroxide further increased the antibody response. Remarkably, the antibody levels 3 or 7 months after the last injection were significantly higher than those 1 wk after that injection. The observed transmission-blocking activity of immune sera correlated with antibody levels measured by ELISA. JF - Proceedings of the National Academy of Sciences, USA AU - Kubler-Kielb, Joanna AU - Majadly, Fathy AU - Wu, Yimin AU - Narum, David L AU - Guo, Chunyan AU - Miller, Louis H AU - Shiloach, Joseph AU - Robbins, John B AU - Schneerson, Rachel AD - Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, MSC 2423, Bethesda, MD 20892-2520 Y1 - 2007/01/02/ PY - 2007 DA - 2007 Jan 02 SP - 293 EP - 298 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 104 IS - 1 SN - 0027-8424, 0027-8424 KW - Pfs25 protein KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Parasites KW - Ovalbumin KW - Mortality KW - Enzyme-linked immunosorbent assay KW - Zygotes KW - Bites KW - Aluminum hydroxide KW - Malaria KW - Plasmodium falciparum KW - Antibody response KW - Development KW - Children KW - exotoxin A KW - thioethers KW - Morbidity KW - Disease transmission KW - Immunogenicity KW - adipic acid KW - Adsorption KW - Midgut KW - Vaccines KW - Pseudomonas aeruginosa KW - amides KW - K 03350:Immunology KW - F 06905:Vaccines KW - J 02350:Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19843979?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Long-lasting+and+transmission-blocking+activity+of+antibodies+to+Plasmodium+falciparum+elicited+in+mice+by+protein+conjugates+of+Pfs25&rft.au=Kubler-Kielb%2C+Joanna%3BMajadly%2C+Fathy%3BWu%2C+Yimin%3BNarum%2C+David+L%3BGuo%2C+Chunyan%3BMiller%2C+Louis+H%3BShiloach%2C+Joseph%3BRobbins%2C+John+B%3BSchneerson%2C+Rachel&rft.aulast=Kubler-Kielb&rft.aufirst=Joanna&rft.date=2007-01-02&rft.volume=104&rft.issue=1&rft.spage=293&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Mortality; Ovalbumin; Parasites; Enzyme-linked immunosorbent assay; Bites; Zygotes; Aluminum hydroxide; Malaria; Development; Antibody response; Children; exotoxin A; Morbidity; thioethers; Disease transmission; Immunogenicity; adipic acid; Adsorption; Vaccines; Midgut; amides; Plasmodium falciparum; Pseudomonas aeruginosa ER - TY - JOUR T1 - Effects of surface electrical stimulation both at rest and during swallowing in chronic pharyngeal Dysphagia. AN - 85404731; pmid-16718620 AB - We tested two hypotheses using surface electrical stimulation in chronic pharyngeal dysphagia: that stimulation (1) lowered the hyoid bone and/or larynx when applied at rest, and (2) increased aspiration, penetration, or pharyngeal pooling during swallowing. Bipolar surface electrodes were placed on the skin overlying the submandibular and laryngeal regions. Maximum tolerated levels of stimulation were applied while patients held their mouth closed at rest. Videofluoroscopic recordings were used to measure hyoid movements in the superior-inferior and anterior-posterior dimensions and the subglottic air column position while stimulation was on or off. Patients swallowed 5 ml liquid when stimulation was off, at low sensory stimulation levels, and at maximum tolerated levels (motor). Speech pathologists, blinded to condition, tallied the frequency of aspiration, penetration, pooling, and esophageal entry from videofluorographic recordings of swallows. Only significant (p = 0.0175) hyoid depression occurred during stimulation at rest. Aspiration and pooling were significantly reduced only with low sensory threshold levels of stimulation (p = 0.025) and not during maximum levels of surface electrical stimulation. Those patients who had reduced aspiration and penetration during swallowing with stimulation had greater hyoid depression during stimulation at rest (p = 0.006). Stimulation may have acted to resist patients' hyoid elevation during swallowing. JF - Dysphagia AU - Ludlow, Christy L AU - Humbert, Ianessa AU - Saxon, Keith AU - Poletto, Christopher AU - Sonies, Barbara AU - Crujido, Lisa AD - Laryngeal and Speech Section, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA. ludlowc@ninds.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1 EP - 10 VL - 22 IS - 1 SN - 0179-051X, 0179-051X KW - Index Medicus KW - National Library of Medicine KW - Adult KW - Aged KW - Chronic Disease KW - *Deglutition: physiology KW - *Deglutition Disorders: therapy KW - *Electric Stimulation: instrumentation KW - Female KW - Humans KW - Hyoid Bone: innervation KW - Larynx KW - Male KW - Middle Aged KW - *Pharynx: physiology KW - Pilot Projects KW - *Rest UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/85404731?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acomdisdome&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Dysphagia&rft.atitle=Effects+of+surface+electrical+stimulation+both+at+rest+and+during+swallowing+in+chronic+pharyngeal+Dysphagia.&rft.au=Ludlow%2C+Christy+L%3BHumbert%2C+Ianessa%3BSaxon%2C+Keith%3BPoletto%2C+Christopher%3BSonies%2C+Barbara%3BCrujido%2C+Lisa&rft.aulast=Ludlow&rft.aufirst=Christy&rft.date=2007-01-01&rft.volume=22&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Dysphagia&rft.issn=0179051X&rft_id=info:doi/ LA - English (eng) DB - ComDisDome N1 - Date revised - 2011-12-15 N1 - SuppNotes - Cites: Laryngoscope. 2002 Dec;112(12):2204-10[12461342]; Cites: J Appl Physiol. 2003 Jan;94(1):128-34[12486019]; Cites: Dysphagia. 1997 Summer;12(3):161-6[9190102]; Cites: Neurogastroenterol Motil. 2003 Feb;15(1):69-77[12588471]; Cites: Am J Physiol Gastrointest Liver Physiol. 2004 Jan;286(1):G45-50[12946939]; Cites: J Auton Nerv Syst. 1984 May-Jun;10(3-4):225-33[6384335]; Cites: Dysphagia. 1996 Spring;11(2):93-8[8721066]; Cites: Respir Care. 2001 May;46(5):466-74[11309186]; Cites: J Psychiatr Res. 1975 Nov;12(3):189-98[1202204]; Cites: J Physiol. 2003 Jul 1;550(Pt 1):287-304[12754311] N1 - Last updated - 2012-07-13 ER - TY - JOUR T1 - Regulation of Cartilaginous ECM Gene Transcription by Chondrocytes and MSCs in 3D Culture in Response to Dynamic Loading AN - 754884821; 13416360 AB - This study explored the biologic response of chondrocytes and mesenchymal stem cells (MSCs) to a dynamic mechanical loading regime. We developed a time-efficient methodology for monitoring regional changes in extracellular matrix gene transcription using reporter promoter constructs. Specifically, transfected cells were homogenously distributed throughout agarose hydrogel constructs, and spatial and temporal gene expression and the ability to form functional ECM were analyzed in response to dynamic mechanical stimuli. Theoretical analyses were used to predict the physical signals generated within the gel in response to these loading regimes. Using a custom compression bioreactor system, changes in aggrecan and type II collagen promoter activity in transfected chondrocyte-laden cylindrical constructs were evaluated in response to a range of loading frequencies and durations. In general, aggrecan promoter activity increased with increasing duration of loading, particularly in the outer annulus region. Interestingly, type II collagen promoter activity decreased in this annular region under identical loading conditions. In addition, we explored the role of mechanical compression in directing chondrogenic differentiation of MSCs by monitoring short-term aggrecan promoter activity. As an example of long-term utility, a specific loading protocol was applied to MSC-laden constructs for 5days, and the resultant changes in glycosaminoglycan (GAG) production were evaluated over a 4-week period. This dynamic loading regime increased not only short-term aggrecan transcriptional activity but also GAG deposition in long-term culture. These results demonstrate the utility of a new reporter promoter system for optimizing loading protocols to improve the outcome of engineered chondrocyte- and MSC-laden cartilaginous constructs. JF - Biomechanics and Modeling in Mechanobiology AU - Mauck, R L AU - Byers, BA AU - Yuan, X AU - Tuan, R S AD - Department of Health and Human Services Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Drive, MSC 8022, Building 50, Room 1503, Bethesda, MD, 20892-8022, USA, tuanr@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 113 EP - 125 PB - Springer-Verlag (Heidelberg), Tiergartenstrasse 17 Heidelberg 69121 Germany VL - 6 IS - 1-2 SN - 1617-7959, 1617-7959 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts; Calcium & Calcified Tissue Abstracts KW - Mechanical loading KW - Chondrocytes KW - Transcription KW - Cell culture KW - Compression KW - Mechanical stimuli KW - Gene expression KW - Promoters KW - Differentiation KW - Stem cells KW - Glycosaminoglycans KW - hydrogels KW - Bioreactors KW - Extracellular matrix KW - Collagen (type II) KW - Mesenchyme KW - aggrecan KW - Mechanical properties KW - N3 11023:Neurogenetics KW - T 2030:Cartilage and Cartilage Diseases KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/754884821?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomechanics+and+Modeling+in+Mechanobiology&rft.atitle=Regulation+of+Cartilaginous+ECM+Gene+Transcription+by+Chondrocytes+and+MSCs+in+3D+Culture+in+Response+to+Dynamic+Loading&rft.au=Mauck%2C+R+L%3BByers%2C+BA%3BYuan%2C+X%3BTuan%2C+R+S&rft.aulast=Mauck&rft.aufirst=R&rft.date=2007-01-01&rft.volume=6&rft.issue=1-2&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Biomechanics+and+Modeling+in+Mechanobiology&rft.issn=16177959&rft_id=info:doi/10.1007%2Fs10237-006-0042-1 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-09-01 N1 - Last updated - 2015-03-19 N1 - SubjectsTermNotLitGenreText - Mechanical loading; Transcription; Chondrocytes; Cell culture; Mechanical stimuli; Compression; Gene expression; Differentiation; Promoters; Stem cells; Glycosaminoglycans; hydrogels; Extracellular matrix; Bioreactors; Collagen (type II); aggrecan; Mesenchyme; Mechanical properties DO - http://dx.doi.org/10.1007/s10237-006-0042-1 ER - TY - JOUR T1 - Parallel analysis of tetramerization domain mutants of the human p53 protein using PCR colonies. AN - 733744408; 18923936 AB - A highly-parallel yeast functional assay, capable of screening approximately 100-1,000 mutants in parallel and designed to screen the activity of transcription activator proteins, was utilized to functionally characterize tetramerization domain mutants of the human p53 transcription factor and tumor suppressor protein. A library containing each of the 19 possible single amino acid substitutions (57 mutants) at three positions in the tetramerization domain of the human p53 protein, was functionally screened in Saccharomyces cerevisiae. Amino acids Leu330 and Ile332, whose side chains form a portion of a hydrophobic pocket that stabilizes the active p53 tetramer, were found to tolerate most hydrophobic amino acid substitutions while hydrophilic substitutions resulted in the inactivation of the protein. Amino acid Gln331 tolerated essentially all mutations. Importantly, highly parallel mutagenesis and cloning techniques were utilized which, in conjunction with recently reported highly parallel DNA sequencing methods, would be capable of increasing throughput an additional 2-3 orders of magnitude. JF - Genomic medicine AU - Merritt, Joshua AU - Roberts, Kim G AU - Butz, James A AU - Edwards, Jeremy S AD - Vaccine Research Center, NIAID, NIH, Bethesda, MD, 20892, USA, merrittj@niaid.nih.gov. Y1 - 2007 PY - 2007 DA - 2007 SP - 113 EP - 124 VL - 1 IS - 3-4 SN - 1871-7934, 1871-7934 UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/733744408?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Genomic+medicine&rft.atitle=Parallel+analysis+of+tetramerization+domain+mutants+of+the+human+p53+protein+using+PCR+colonies.&rft.au=Merritt%2C+Joshua%3BRoberts%2C+Kim+G%3BButz%2C+James+A%3BEdwards%2C+Jeremy+S&rft.aulast=Merritt&rft.aufirst=Joshua&rft.date=2007-01-01&rft.volume=1&rft.issue=3-4&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Genomic+medicine&rft.issn=18717934&rft_id=info:doi/10.1007%2Fs11568-007-9011-8 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2011-07-14 N1 - Date created - 2008-10-16 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Biotechnol Bioeng. 2004 Apr 20;86(2):117-24 [15052631] Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9934-9 [12909720] Anal Biochem. 2003 Sep 1;320(1):55-65 [12895469] Nucleic Acids Res. 2003 Aug 1;31(15):e84 [12888536] Mol Cell Biol. 2002 Dec;22(24):8612-25 [12446780] J Mol Microbiol Biotechnol. 2002 Nov;4(6):539-50 [12432954] Oncogene. 2001 Jun 14;20(27):3573-9 [11429705] Oncogene. 2001 May 10;20(21):2611-7 [11420672] Nature. 2001 Feb 15;409(6822):860-921 [11237011] Science. 2001 Feb 16;291(5507):1304-51 [11181995] Int J Cancer. 1999 Dec 22;84(6):587-93 [10567903] Nucleic Acids Res. 1999 Dec 15;27(24):e34 [10572186] Biotechnol Bioeng. 2005 Dec 5;92(5):519-31 [16193512] Science. 2005 Sep 9;309(5741):1728-32 [16081699] Proc Natl Acad Sci U S A. 2005 May 3;102(18):6431-6 [15843459] Hum Mutat. 1999;14(1):1-8 [10447253] J Mol Biol. 1999 May 21;288(5):891-7 [10329187] Nature. 1999 Apr 15;398(6728):545-6 [10217129] Int J Cancer. 1998 Oct 29;78(3):372-6 [9766574] Mol Carcinog. 1997 Aug;19(4):243-53 [9290701] Genes Dev. 1996 May 1;10(9):1054-72 [8654922] J Biol Chem. 1996 Apr 26;271(17):10017-22 [8626555] Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3963-7 [7732013] Nucleic Acids Res. 1992 Apr 11;20(7):1539-45 [1579447] Science. 1991 Jun 21;252(5013):1708-11 [2047879] Metab Eng. 2004 Jul;6(3):212-9 [15256211] N1 - Last updated - 2017-01-18 DO - http://dx.doi.org/10.1007/s11568-007-9011-8 ER - TY - CONF T1 - Workshop on imaging science development for cancer prevention and preemption. AN - 70759456; 17655039 AB - The concept of intraepithelial neoplasm (IEN) as a near-obligate precursor of cancers has generated opportunities to examine drug or device intervention strategies that may reverse or retard the sometimes lengthy process of carcinogenesis. Chemopreventive agents with high therapeutic indices, well-monitored for efficacy and safety, are greatly needed, as is development of less invasive or minimally disruptive visualization and assessment methods to safely screen nominally healthy but at-risk patients, often for extended periods of time and at repeated intervals. Imaging devices, alone or in combination with anticancer drugs, may also provide novel interventions to treat or prevent precancer. JF - Cancer biomarkers : section A of Disease markers AU - Kelloff, Gary J AU - Sullivan, Daniel C AU - Baker, Houston AU - Clarke, Lawrence P AU - Nordstrom, Robert AU - Tatum, James L AU - Dorfman, Gary S AU - Jacobs, Paula AU - Berg, Christine D AU - Pomper, Martin G AU - Birrer, Michael J AU - Tempero, Margaret AU - Higley, Howard R AU - Petty, Brenda Gumbs AU - Sigman, Caroline C AU - Maley, Carlo AU - Sharma, Prateek AU - Wax, Adam AU - Ginsberg, Gregory G AU - Dannenberg, Andrew J AU - Hawk, Ernest T AU - Messing, Edward M AU - Grossman, H Barton AU - Harisinghani, Mukesh AU - Bigio, Irving J AU - Griebel, Donna AU - Henson, Donald E AU - Fabian, Carol J AU - Ferrara, Katherine AU - Fantini, Sergio AU - Schnall, Mitchell D AU - Zujewski, Jo Anne AU - Hayes, Wendy AU - Klein, Eric A AU - DeMarzo, Angelo AU - Ocak, Iclal AU - Ketterling, Jeffrey A AU - Tempany, Clare AU - Shtern, Faina AU - Parnes, Howard L AU - Gomez, Jorge AU - Srivastava, Sudhir AU - Szabo, Eva AU - Lam, Stephen AU - Seibel, Eric J AU - Massion, Pierre AU - McLennan, Geoffrey AU - Cleary, Kevin AU - Suh, Robert AU - Burt, Randall W AU - Pfeiffer, Ruth M AU - Hoffman, John M AU - Roy, Hemant K AU - Wang, Thomas AU - Limburg, Paul J AU - El-Deiry, Wafik S AU - Papadimitrakopoulou, Vali AU - Hittelman, Walter N AU - MacAulay, Calum AU - Veltri, Robert W AU - Solomon, Diane AU - Jeronimo, Jose AU - Richards-Kortum, Rebecca AU - Johnson, Karen A AU - Viner, Jaye L AU - Stratton, Steven P AU - Rajadhyaksha, Milind AU - Dhawan, Atam AU - Workshop Program Committee Y1 - 2007 PY - 2007 DA - 2007 SP - 1 EP - 33 VL - 3 IS - 1 KW - Index Medicus KW - Image Interpretation, Computer-Assisted KW - Humans KW - Neoplasms -- diagnosis KW - Precancerous Conditions -- prevention & control KW - Precancerous Conditions -- diagnosis KW - Neoplasms -- prevention & control KW - Diagnostic Imaging -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70759456?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Cancer+biomarkers+%3A+section+A+of+Disease+markers&rft.atitle=Workshop+on+imaging+science+development+for+cancer+prevention+and+preemption.&rft.au=Kelloff%2C+Gary+J%3BSullivan%2C+Daniel+C%3BBaker%2C+Houston%3BClarke%2C+Lawrence+P%3BNordstrom%2C+Robert%3BTatum%2C+James+L%3BDorfman%2C+Gary+S%3BJacobs%2C+Paula%3BBerg%2C+Christine+D%3BPomper%2C+Martin+G%3BBirrer%2C+Michael+J%3BTempero%2C+Margaret%3BHigley%2C+Howard+R%3BPetty%2C+Brenda+Gumbs%3BSigman%2C+Caroline+C%3BMaley%2C+Carlo%3BSharma%2C+Prateek%3BWax%2C+Adam%3BGinsberg%2C+Gregory+G%3BDannenberg%2C+Andrew+J%3BHawk%2C+Ernest+T%3BMessing%2C+Edward+M%3BGrossman%2C+H+Barton%3BHarisinghani%2C+Mukesh%3BBigio%2C+Irving+J%3BGriebel%2C+Donna%3BHenson%2C+Donald+E%3BFabian%2C+Carol+J%3BFerrara%2C+Katherine%3BFantini%2C+Sergio%3BSchnall%2C+Mitchell+D%3BZujewski%2C+Jo+Anne%3BHayes%2C+Wendy%3BKlein%2C+Eric+A%3BDeMarzo%2C+Angelo%3BOcak%2C+Iclal%3BKetterling%2C+Jeffrey+A%3BTempany%2C+Clare%3BShtern%2C+Faina%3BParnes%2C+Howard+L%3BGomez%2C+Jorge%3BSrivastava%2C+Sudhir%3BSzabo%2C+Eva%3BLam%2C+Stephen%3BSeibel%2C+Eric+J%3BMassion%2C+Pierre%3BMcLennan%2C+Geoffrey%3BCleary%2C+Kevin%3BSuh%2C+Robert%3BBurt%2C+Randall+W%3BPfeiffer%2C+Ruth+M%3BHoffman%2C+John+M%3BRoy%2C+Hemant+K%3BWang%2C+Thomas%3BLimburg%2C+Paul+J%3BEl-Deiry%2C+Wafik+S%3BPapadimitrakopoulou%2C+Vali%3BHittelman%2C+Walter+N%3BMacAulay%2C+Calum%3BVeltri%2C+Robert+W%3BSolomon%2C+Diane%3BJeronimo%2C+Jose%3BRichards-Kortum%2C+Rebecca%3BJohnson%2C+Karen+A%3BViner%2C+Jaye+L%3BStratton%2C+Steven+P%3BRajadhyaksha%2C+Milind%3BDhawan%2C+Atam%3BWorkshop+Program+Committee&rft.aulast=Kelloff&rft.aufirst=Gary&rft.date=2007-01-01&rft.volume=3&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Cancer+biomarkers+%3A+section+A+of+Disease+markers&rft.issn=15740153&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-08-14 N1 - Date created - 2007-07-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Physiological and pathological brain hyperthermia. AN - 70751625; 17645922 AB - While brain temperature is usually considered a stable, tightly regulated parameter, recent animal research revealed relatively large and rapid brain temperature fluctuations (approximately 3 degrees C) during various forms of naturally occurring physiological and behavioral activities. This work demonstrates that physiological brain hyperthermia has an intra-brain origin, resulting from enhanced neural metabolism and increased intra-brain heat production, and discusses its possible mechanisms and functional consequences. This work also shows that brain hyperthermia may also be induced by various drugs of abuse. While each individual drug (i.e., heroin, cocaine, meth-amphetamine, MDMA) has its own, dose-dependent effects on brain and body temperatures, these effects are strongly modulated by the individual's activity state and environmental conditions, showing dramatic alterations during the development of drug-taking behavior. While brain temperatures may also increase due to environmental overheating and diminished heat dissipation from the brain, adverse environmental conditions and physiological activation strongly potentiate thermal effects of psychomotor stimulant drugs, resulting in dangerous brain overheating. Since hyperthermia exacerbates drug-induced toxicity and is destructive to neural cells and brain functions, use of these drugs under conditions that restrict heat loss may pose a significant health risk, resulting in both acute life-threatening complications and chronic destructive CNS changes. We argue that brain temperature is an important physiological parameter, affecting various neural functions, and show the potential of brain temperature monitoring for studying alterations in metabolic neural activity under physiological and pathological conditions. Finally, we discuss brain temperature as a factor affecting various neuronal and neurochemical evaluations made in different animal preparations (in vitro slices, general anesthesia, awake, freely moving conditions) and consider a possible contribution of temperature fluctuations to behavior-related and drug-induced alterations in neuronal and neurochemical parameters. JF - Progress in brain research AU - Kiyatkin, Eugene A AD - Cellular Neurobiology Branch, National Institute on Drug Abuse - Intramural Research Program, National Institutes of Health, DHHS, Baltimore, MD 21224, USA. ekiyatki@intra.nida.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 219 EP - 243 VL - 162 SN - 0079-6123, 0079-6123 KW - Index Medicus KW - Animals KW - Humans KW - Fever -- pathology KW - Brain -- pathology KW - Fever -- physiopathology KW - Brain -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70751625?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+brain+research&rft.atitle=Physiological+and+pathological+brain+hyperthermia.&rft.au=Kiyatkin%2C+Eugene+A&rft.aulast=Kiyatkin&rft.aufirst=Eugene&rft.date=2007-01-01&rft.volume=162&rft.issue=&rft.spage=219&rft.isbn=&rft.btitle=&rft.title=Progress+in+brain+research&rft.issn=00796123&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-04-07 N1 - Date created - 2007-07-24 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Multiple functions for the N-terminal region of Msh6. AN - 70716903; 17567610 AB - The eukaryotic mismatch repair protein Msh6 shares five domains in common with other MutS members. However, it also contains several hundred additional residues at its N-terminus. A few of these residues bind to PCNA, but the functions of the other amino acids in the N-terminal region (NTR) are unknown. Here we demonstrate that the Msh6 NTR binds to duplex DNA in a salt-sensitive, mismatch-independent manner. Partial proteolysis, DNA affinity chromatography and mass spectrometry identified a fragment comprised of residues 228-299 of yeast Msh6 that binds to DNA and is rich in positively charged residues. Deleting these residues, or replacing lysines and arginines with glutamate, reduces DNA binding in vitro and elevates spontaneous mutation rates and resistance to MNNG treatment in vivo. Similar in vivo defects are conferred by alanine substitutions in a highly conserved motif in the NTR that immediately precedes domain I of MutS proteins, the domain that interacts with mismatched DNA. These data suggest that, in addition to PCNA binding, DNA binding and possibly other functions in the amino terminal region of Msh6 are important for eukaryotic DNA mismatch repair and cellular response to alkylation damage. JF - Nucleic acids research AU - Clark, Alan B AU - Deterding, Leesa AU - Tomer, Kenneth B AU - Kunkel, Thomas A AD - Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 4114 EP - 4123 VL - 35 IS - 12 KW - DNA-Binding Proteins KW - 0 KW - G-T mismatch-binding protein KW - MSH6 protein, S cerevisiae KW - Peptides KW - Saccharomyces cerevisiae Proteins KW - Methylnitronitrosoguanidine KW - 12H3O2UGSF KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Methylnitronitrosoguanidine -- toxicity KW - DNA Mismatch Repair KW - Conserved Sequence KW - Amino Acid Motifs KW - DNA -- metabolism KW - Molecular Sequence Data KW - Peptides -- metabolism KW - Amino Acid Sequence KW - Protein Structure, Tertiary KW - Mutation, Missense KW - Sequence Deletion KW - Binding Sites KW - Saccharomyces cerevisiae Proteins -- metabolism KW - DNA-Binding Proteins -- chemistry KW - Saccharomyces cerevisiae Proteins -- genetics KW - DNA-Binding Proteins -- genetics KW - Saccharomyces cerevisiae Proteins -- chemistry KW - DNA-Binding Proteins -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70716903?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+acids+research&rft.atitle=Multiple+functions+for+the+N-terminal+region+of+Msh6.&rft.au=Clark%2C+Alan+B%3BDeterding%2C+Leesa%3BTomer%2C+Kenneth+B%3BKunkel%2C+Thomas+A&rft.aulast=Clark&rft.aufirst=Alan&rft.date=2007-01-01&rft.volume=35&rft.issue=12&rft.spage=4114&rft.isbn=&rft.btitle=&rft.title=Nucleic+acids+research&rft.issn=1362-4962&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-08-07 N1 - Date created - 2007-07-10 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Int J Cancer. 2000 Mar 1;85(5):606-13 [10699937] Cancer Res. 1999 Oct 15;59(20):5068-74 [10537275] Nature. 2000 Oct 12;407(6805):711-7 [11048711] Nat Genet. 2000 Nov;26(3):375-8 [11062484] J Biol Chem. 2000 Nov 24;275(47):36498-501 [11005803] Genes Dev. 2001 Mar 15;15(6):724-36 [11274057] J Mol Biol. 2001 Sep 28;312(4):637-47 [11575920] Free Radic Biol Med. 2002 Aug 1;33(3):364-9 [12126758] J Mol Biol. 2003 Jul 11;330(3):571-6 [12842472] Annu Rev Microbiol. 2003;57:579-608 [14527292] Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14822-7 [14634210] Genet Eng (N Y). 2003;25:189-207 [15260239] Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6424-8 [8341649] Mol Cell Biol. 1998 Apr;18(4):1891-902 [9528760] Nucleic Acids Res. 1999 Feb 1;27(3):736-42 [9889267] Mol Cell Biol. 1999 Mar;19(3):2000-7 [10022887] Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2970-5 [10077621] Am J Hum Genet. 1999 Nov;65(5):1291-8 [10521294] Annu Rev Biochem. 2005;74:681-710 [15952900] Curr Biol. 2005 Aug 9;15(15):1395-400 [16085492] DNA Repair (Amst). 2005 Dec 8;4(12):1410-20 [16226493] Chem Rev. 2006 Feb;106(2):302-23 [16464007] Nat Rev Mol Cell Biol. 2006 May;7(5):335-46 [16612326] Nature. 2000 Oct 12;407(6805):703-10 [11048710] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Biotechnology and the treatment of addictive disorders: new opportunities. AN - 70716870; 17628123 AB - Addiction is a chronic relapsing illness with onset typically occurring in the early teenage years, followed by cycles of drug use and abstinence. The disease is mitigated by complex interactions between genes and environment. Viewed as such, the treatment of addiction could span the whole lifetime of the patient and, ideally, should be tailored to the illness cycle. The search for effective treatments has intensified recently due to our better understanding of the underlying neurobiologic mechanisms contributing to drug use and relapse. The three main types of treatment are behavioral, pharmacologic and, more recently, immunologic therapies. Vaccines and monoclonal antibodies are being developed mainly for stimulant use disorders and nicotine addiction. In addition, new molecular targets identified by preclinical research have shown promise and are awaiting proof-of-concept studies in humans. The main focus of this review is on the development of immunotherapy for stimulants and nicotine addiction as a model highlighting the current status of the science and potential emerging discoveries and development. JF - BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy AU - Elkashef, Ahmed AU - Biswas, Jamie AU - Acri, Jane B AU - Vocci, Frank AD - Division of Pharmacotherapies and Medical Consequences of Drug Abuse (DPMC), National Institute on Drug Abuse (NIDA), National Institutes of Health (NIH), Bethesda, Maryland 20892-9551, USA. ae8a@nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 259 EP - 267 VL - 21 IS - 4 SN - 1173-8804, 1173-8804 KW - Antibodies, Monoclonal KW - 0 KW - Immunologic Factors KW - Vaccines KW - Index Medicus KW - Vaccines -- immunology KW - Amphetamine-Related Disorders -- epidemiology KW - Tobacco Use Disorder -- therapy KW - Humans KW - Immunotherapy KW - Tobacco Use Disorder -- epidemiology KW - Tobacco Use Disorder -- immunology KW - Cocaine-Related Disorders -- therapy KW - Cocaine-Related Disorders -- epidemiology KW - Amphetamine-Related Disorders -- immunology KW - Vaccines -- therapeutic use KW - Antibodies, Monoclonal -- therapeutic use KW - Immunologic Factors -- therapeutic use KW - Biotechnology KW - Cocaine-Related Disorders -- immunology KW - Amphetamine-Related Disorders -- therapy KW - Substance-Related Disorders -- therapy KW - Substance-Related Disorders -- immunology KW - Substance-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70716870?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BioDrugs+%3A+clinical+immunotherapeutics%2C+biopharmaceuticals+and+gene+therapy&rft.atitle=Biotechnology+and+the+treatment+of+addictive+disorders%3A+new+opportunities.&rft.au=Elkashef%2C+Ahmed%3BBiswas%2C+Jamie%3BAcri%2C+Jane+B%3BVocci%2C+Frank&rft.aulast=Elkashef&rft.aufirst=Ahmed&rft.date=2007-01-01&rft.volume=21&rft.issue=4&rft.spage=259&rft.isbn=&rft.btitle=&rft.title=BioDrugs+%3A+clinical+immunotherapeutics%2C+biopharmaceuticals+and+gene+therapy&rft.issn=11738804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-20 N1 - Date created - 2007-07-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Complementary and alternative medicine (CAM) for the treatment of chronic hepatitis B and C: a review. AN - 70649734; 17591018 AB - Complementary and alternative medicine (CAM) has been used for centuries in China and Japan to treat various illnesses, including viral hepatitis. Several therapeutic approaches constitute CAM, the most relevant for this review being the use of herbals. However, profound disagreements exist between conventional and alternative medicine practitioners regarding their value. Western medical advocates cite deep concerns about the purity of most herbals because of lack of standardized production, the paucity of pharmacokinetic data, the fact that few well-designed randomized, controlled trials of these products have been performed and the evidence that some herbals have been responsible for severe adverse effects. Nevertheless, many in the public, even in western countries, turn to the use of herbals, believing that they must be safe and effective because they are 'natural' and have been used for centuries, and because of dissatisfaction with conventional medicine. Accordingly, their use in western countries and the costs incurred have increased each year. While there is evidence that some herbals have physiological effects, there still is insufficient evidence to recommend their use. This paper reviews the classification, epidemiology and philosophy of CAM, and the reasons advanced for herbal use to treat viral hepatitis. The criteria necessary to develop a potential pharmacological agent are presented, as well as the requirements for conducting a scientifically valid treatment trial of herbals. Five herbals used in the past to treat viral hepatitis are reviewed and evaluated for the quality of their studies and mention is made of herbals known to have adverse effects. JF - Antiviral therapy AU - Modi, Apurva A AU - Wright, Elizabeth C AU - Seeff, Leonard B AD - The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institute of Health, Bethesda, MD, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 285 EP - 295 VL - 12 IS - 3 SN - 1359-6535, 1359-6535 KW - Plant Preparations KW - 0 KW - Index Medicus KW - Global Health KW - Animals KW - Phytotherapy -- economics KW - Costs and Cost Analysis KW - Plant Preparations -- adverse effects KW - Humans KW - Chemical and Drug Induced Liver Injury KW - Health Surveys KW - Herb-Drug Interactions KW - Drug Evaluation, Preclinical KW - Phytotherapy -- adverse effects KW - Hepatitis B, Chronic -- therapy KW - Complementary Therapies -- economics KW - Complementary Therapies -- classification KW - Complementary Therapies -- adverse effects KW - Hepatitis C, Chronic -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70649734?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antiviral+therapy&rft.atitle=Complementary+and+alternative+medicine+%28CAM%29+for+the+treatment+of+chronic+hepatitis+B+and+C%3A+a+review.&rft.au=Modi%2C+Apurva+A%3BWright%2C+Elizabeth+C%3BSeeff%2C+Leonard+B&rft.aulast=Modi&rft.aufirst=Apurva&rft.date=2007-01-01&rft.volume=12&rft.issue=3&rft.spage=285&rft.isbn=&rft.btitle=&rft.title=Antiviral+therapy&rft.issn=13596535&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-08-06 N1 - Date created - 2007-06-26 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The use of bisphosphonates in cancer patients. AN - 70605666; 17562434 AB - Skeletal-related events resulting from bone metastases or osteoporosis can significantly contribute to morbidity and mortality in cancer patients. Expert opinion on the effectiveness of bisphosphonates in this setting is evolving. Here we review current evidence on the risks and benefits of bisphosphonate therapy for a wide variety of cancers, as well as clinical management of its adverse effects. A MEDLINE search of English-language literature (1966 through May 2006) was conducted using the terms bisphosphonate, cancer, multiple myeloma, malignancy, and randomized controlled clinical studies. Studies were selected based on clinical pertinence, with an emphasis on phase III clinical trials. We reviewed bibliographies for other relevant articles. Accumulating evidence reveals that bisphosphonate therapy has a significant effect in preventing skeletal complications in multiple myeloma, breast cancers, and prostate cancer, and in reducing skeletal complications in other metastatic bone malignancies. Emerging data indicate that bisphosphonates are useful for preventing bone loss resulting from cancer or its therapy. The efficacy of bisphosphonates for early-stage breast cancers remains controversial. Significant risks of bisphosphonate therapy include nephrotoxicity, electrolyte abnormalities, and osteonecrosis of the jaw. Bisphosphonate therapy has a clear role in the management of skeletal metastases associated with a variety of cancers. However, significant side effects require ongoing monitoring and treatment. JF - Acta oncologica (Stockholm, Sweden) AU - Wu, Shenhong AU - Dahut, William L AU - Gulley, James L AD - Medical Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 581 EP - 591 VL - 46 IS - 5 SN - 0284-186X, 0284-186X KW - Bone Density Conservation Agents KW - 0 KW - Diphosphonates KW - Index Medicus KW - Humans KW - Diphosphonates -- therapeutic use KW - Diphosphonates -- adverse effects KW - Bone Density Conservation Agents -- therapeutic use KW - Bone Diseases, Metabolic -- etiology KW - Bone Diseases, Metabolic -- drug therapy KW - Bone Neoplasms -- secondary KW - Bone Neoplasms -- complications KW - Bone Density Conservation Agents -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70605666?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.atitle=The+use+of+bisphosphonates+in+cancer+patients.&rft.au=Wu%2C+Shenhong%3BDahut%2C+William+L%3BGulley%2C+James+L&rft.aulast=Wu&rft.aufirst=Shenhong&rft.date=2007-01-01&rft.volume=46&rft.issue=5&rft.spage=581&rft.isbn=&rft.btitle=&rft.title=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.issn=0284186X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-06 N1 - Date created - 2007-06-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Cancer survivorship research: state of knowledge, challenges and opportunities. AN - 70490470; 17497308 AB - Seminal advances in early detection of and treatment strategies for cancer have led to burgeoning numbers of cancer survivors. While most therapeutic modalities for cancer are beneficial and lifesaving, they are associated with adverse long-term and late sequelae. Literature review using MEDLINE to identify studies examining adverse medical outcomes and post-treatment follow-up care among long-term survivors. Emerging concepts in survivorship research such as definitional issues, research paradigms and methodologic concerns were also examined. Long-term or late adverse sequelae are more prevalent, serious, and persistent than expected in survivors of pediatric and adult cancer, but remain understudied especially among those diagnosed as adults. Follow-up care relevant to survivorship outcomes is neither standardized nor guideline or evidence based for most adult cancers, and optimal practices have yet to be defined. Adverse sequelae contribute to burden of illness, health care costs, and decreased length and quality of survival. To-date, very few studies have compared survivor outcomes pre-and post diagnosis. It is critical to examine under-researched questions and understudied survivor groups. Regular follow-up care and monitoring of health status post cancer treatment should 1) permit the timely diagnosis and treatment of adverse outcomes; 2) enable timely diagnosis and treatment of recurrences; 3) facilitate screening and early detection of second cancer(s); 4) allow for detection and management of co-morbidities; and 5) provide the opportunity for preventive strategies such as lifestyle changes. Research findings to-date underscore the need for continued cancer survivorship research that will: inform our understanding of the mechanisms underlying adverse sequelae; lead to the design of less toxic treatments; test the effectiveness of interventions - medical, pharmacologic, and behavioral - that reduce adverse outcomes; test models of post-treatment follow-up care; develop an evidence base for optimal follow-up care practices; and inform survivor and provider decision making. JF - Acta oncologica (Stockholm, Sweden) AU - Aziz, Noreen M AD - Office of Cancer Survivorship, Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, Maryland 20892, USA. na45f@nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 417 EP - 432 VL - 46 IS - 4 SN - 0284-186X, 0284-186X KW - Index Medicus KW - Age Factors KW - Comorbidity -- trends KW - Humans KW - Drug-Related Side Effects and Adverse Reactions KW - Treatment Outcome KW - Radiotherapy -- adverse effects KW - Continuity of Patient Care KW - Neoplasms -- diagnosis KW - Neoplasms -- epidemiology KW - Research -- trends KW - Neoplasms -- therapy KW - Survivors UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70490470?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.atitle=Cancer+survivorship+research%3A+state+of+knowledge%2C+challenges+and+opportunities.&rft.au=Aziz%2C+Noreen+M&rft.aulast=Aziz&rft.aufirst=Noreen&rft.date=2007-01-01&rft.volume=46&rft.issue=4&rft.spage=417&rft.isbn=&rft.btitle=&rft.title=Acta+oncologica+%28Stockholm%2C+Sweden%29&rft.issn=0284186X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-03-06 N1 - Date created - 2007-05-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Contrast-enhanced sonography of the kidney. AN - 70477656; 17420958 AB - Contrast-enhanced sonography (CEUS) is a recently introduced, promising technique in the evaluation of the kidney. CEUS allows real-time assessment of normal and abnormal renal perfusions. As a consequence of the macrocirculation analysis allowed by Doppler techniques, it is possible to obtain real-time information about microcirculation. US contrast media are not nephrotoxic and can be employed safely, even in subjects with impaired renal function. There are several clinical scenarios where CEUS may play the role of a low-cost, scarcely invasive tool, including renal tumors (with special reference to small, indeterminate masses, i.e., differentiation between carcinoma and angiomyolipoma), renal atypical cystic masses (i.e., differentiation of malignant from benign cysts and follow-up of cystic lesions managed conservatively), renal infarction, renal infections, and renal injuries. In addition, CEUS can be useful in the assessment of renal pseudotumors (including any case with possible renal mass on conventional US imaging) and has been employed in radiofrequency ablation guidance. This pictorial review illustrates the CEUS findings recognizable in a wide spectrum of renal disorders and discusses the strengths and limitations of renal imaging with CEUS. JF - Abdominal imaging AU - Setola, S V AU - Catalano, O AU - Sandomenico, F AU - Siani, A AD - Department of Radiology, National Cancer Institute Fondazione Pascale, via M.Semmola, Naples, I-80131, Italy. sesetol@tin.it PY - 2007 SP - 21 EP - 28 VL - 32 IS - 1 SN - 0942-8925, 0942-8925 KW - Contrast Media KW - 0 KW - Phospholipids KW - contrast agent BR1 KW - Sulfur Hexafluoride KW - WS7LR3I1D6 KW - Index Medicus KW - Ultrasonography, Interventional KW - Pyelonephritis -- diagnostic imaging KW - Humans KW - Ultrasonography, Doppler -- methods KW - Aged KW - Kidney Neoplasms -- diagnostic imaging KW - Microbubbles KW - Carcinoma -- diagnostic imaging KW - Microcirculation -- diagnostic imaging KW - Adult KW - Kidney Diseases, Cystic -- diagnostic imaging KW - Middle Aged KW - Angiomyolipoma -- diagnostic imaging KW - Infarction -- diagnostic imaging KW - Catheter Ablation KW - Female KW - Male KW - Kidney -- diagnostic imaging KW - Kidney -- injuries KW - Kidney -- blood supply KW - Kidney Diseases -- diagnostic imaging KW - Image Enhancement -- methods KW - Kidney Diseases -- microbiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70477656?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Abdominal+imaging&rft.atitle=Contrast-enhanced+sonography+of+the+kidney.&rft.au=Setola%2C+S+V%3BCatalano%2C+O%3BSandomenico%2C+F%3BSiani%2C+A&rft.aulast=Setola&rft.aufirst=S&rft.date=2007-01-01&rft.volume=32&rft.issue=1&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Abdominal+imaging&rft.issn=09428925&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-27 N1 - Date created - 2007-05-11 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Inhibition of cell proliferation, induction of apoptosis, reactivation of DLC1, and modulation of other gene expression by dietary flavone in breast cancer cell lines. AN - 70468003; 17418982 AB - Dietary flavone was previously shown to increase the expression of deleted in liver cancer-1 gene (DLC-1) in HT-29 colon carcinoma cell line [Herzog A, Kindermann B, Doring F, Daniel H, Wenzel U. Pleiotropic molecular effects of the pro-apoptotic dietary constituent flavone in human colon cancer cells identified by protein and mRNA expression profiling. Proteomics 2004;4:2455-64]. DLC-1 that encodes a Rho GTPase-activating protein, functions as a tumor suppressor gene and is frequently inactivated or down-regulated in several common cancers. Restoration of DLC-1 expression suppresses in vitro tumor cells proliferation and tumorigenicity in vivo. Here, the effect of flavone was examined in several DLC-1-deficient cell lines derived from different types human cancer using assays for cell proliferation, gene expression and transfer. We show that exposure to 150 microM flavone increased DLC1 expression in breast but not in liver or prostate carcinoma cells or a nonmalignant breast epithelial cell line. Flavone restored the expression of DLC1 in the breast carcinoma cell lines MDA-MB-468, MDA-MB-361, and BT20 as well as in the colon carcinoma cell line HT-29 all of which are DLC-1-negative due to promoter hypermethylation. We further show that flavone inhibited cell proliferation, induced cell cycle arrest at G(2)-M, increased p21(Waf1) gene expression, and caused apoptosis. Microarray analysis of these aggressive and metastatic breast carcinoma cells revealed 29 flavone-responsive genes, among which the DNA damage-inducible GADD genes were up-regulated and the proto-oncogene STMN1 and IGFBP3 were down-regulated. Flavone-mediated alterations of genes that regulate tumor cell proliferation, cell cycle, and apoptosis contribute to chemopreventive and antitumoral effects of flavone. Alone or in combination with demethylating agents, flavone may be an effective adjunct to chemotherapy in preventing breast cancer metastasis. JF - Cancer detection and prevention AU - Ullmannova, Veronika AU - Popescu, Nicholas C AD - Laboratory of Experimental Carcinogenesis, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, MSC 4264, Bethesda, MD 20892-4255, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 110 EP - 118 VL - 31 IS - 2 SN - 0361-090X, 0361-090X KW - Biomarkers, Tumor KW - 0 KW - Cyclin-Dependent Kinase Inhibitor p21 KW - DLC1 protein, human KW - Flavones KW - GTPase-Activating Proteins KW - Tumor Suppressor Proteins KW - Caspase 3 KW - EC 3.4.22.- KW - Index Medicus KW - Immunoblotting KW - Oligonucleotide Array Sequence Analysis KW - Genes, Tumor Suppressor KW - Humans KW - Reverse Transcriptase Polymerase Chain Reaction KW - Biomarkers, Tumor -- metabolism KW - Gene Expression Profiling KW - Tumor Cells, Cultured KW - DNA Methylation KW - Cyclin-Dependent Kinase Inhibitor p21 -- metabolism KW - Flow Cytometry KW - Cell Cycle KW - Male KW - Female KW - Caspase 3 -- metabolism KW - Cell Proliferation -- drug effects KW - Breast Neoplasms -- drug therapy KW - Breast Neoplasms -- pathology KW - Tumor Suppressor Proteins -- metabolism KW - Apoptosis -- drug effects KW - Tumor Suppressor Proteins -- genetics KW - Breast Neoplasms -- metabolism KW - Flavones -- pharmacology KW - Gene Expression Regulation, Neoplastic -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70468003?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+detection+and+prevention&rft.atitle=Inhibition+of+cell+proliferation%2C+induction+of+apoptosis%2C+reactivation+of+DLC1%2C+and+modulation+of+other+gene+expression+by+dietary+flavone+in+breast+cancer+cell+lines.&rft.au=Ullmannova%2C+Veronika%3BPopescu%2C+Nicholas+C&rft.aulast=Ullmannova&rft.aufirst=Veronika&rft.date=2007-01-01&rft.volume=31&rft.issue=2&rft.spage=110&rft.isbn=&rft.btitle=&rft.title=Cancer+detection+and+prevention&rft.issn=0361090X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-07-31 N1 - Date created - 2007-05-07 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proteomics. 2004 Aug;4(8):2455-64 [15274140] Nutr Cancer. 2003;45(2):247-55 [12881020] Science. 1991 Jul 5;253(5015):49-53 [1905840] Cancer Res. 1993 Mar 15;53(6):1328-31 [8443813] Biochem Biophys Res Commun. 1994 Oct 28;204(2):578-84 [7980517] Eur J Cancer. 1994;30A(11):1675-82 [7833143] J Natl Cancer Inst. 1996 May 1;88(9):601-6 [8609661] Mol Cell Biol. 1996 Sep;16(9):4952-60 [8756654] Carcinogenesis. 1996 Nov;17(11):2367-75 [8968050] Nutr Cancer. 1997;27(1):31-40 [8970179] Nutr Cancer. 1997;28(3):236-47 [9343831] Leuk Res. 2003 Dec;27(12):1115-23 [12921950] Cancer Res. 2003 Oct 15;63(20):6607-12 [14583453] Cancer Res. 2003 Nov 15;63(22):7563-70 [14633667] Biochem Biophys Res Commun. 2004 Jan 16;313(3):654-65 [14697242] Oncogene. 2004 Feb 12;23(6):1308-13 [14647417] Oncogene. 2004 Feb 19;23(7):1405-11 [14661059] Pancreas. 2004 May;28(4):e90-5 [15097869] J Proteome Res. 2004 Mar-Apr;3(2):218-27 [15113097] Cancer Res. 1998 May 15;58(10):2196-9 [9605766] J Cell Physiol. 2005 Jan;202(1):295-303 [15316935] Am J Physiol Endocrinol Metab. 2005 Jan;288(1):E92-105 [15367391] Int J Oncol. 2005 Jan;26(1):185-9 [15586239] Oncogene. 2005 Feb 17;24(8):1412-22 [15608671] Oncogene. 2005 Mar 3;24(10):1774-87 [15674352] Cancer Res. 2005 Jul 15;65(14):6042-53 [16024604] Oncogene. 2005 Aug 4;24(33):5246-51 [15897880] Carcinogenesis. 2005 Oct;26(10):1793-803 [15905199] Cancer Res. 2005 Oct 1;65(19):8861-8 [16204057] Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):7033-41 [16203797] Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15207-12 [16217026] Clin Cancer Res. 2005 Oct 15;11(20):7434-43 [16243817] Drug Metab Dispos. 2006 Feb;34(2):296-304 [16280456] Clin Cancer Res. 2006 Mar 1;12(5):1412-9 [16533763] Cancer Res. 2006 Apr 1;66(7):3347-50 [16585150] Anticancer Res. 2006 Mar-Apr;26(2A):1039-48 [16619504] J Mol Diagn. 2006 May;8(2):218-24 [16645208] Carcinogenesis. 2007 Jan;28(1):60-70 [16774933] Oncogene. 2007 Feb 8;26(6):934-44 [16862168] Oncogene. 2007 Feb 15;26(7):1003-12 [16909102] Cancer Lett. 2000 Aug 1;156(1):37-44 [10840157] Nature. 2000 Nov 16;408(6810):307-10 [11099028] Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):781-3 [11158542] Clin Cancer Res. 2001 Feb;7(2):382-90 [11234894] Cancer Epidemiol Biomarkers Prev. 2001 May;10(5):483-8 [11352858] Cancer Res. 2002 Mar 1;62(5):1289-95 [11888893] Carcinogenesis. 2002 Sep;23(9):1491-6 [12189192] Cancer Res. 2002 Dec 1;62(23):6864-9 [12460900] Oncogene. 2003 Jan 23;22(3):445-50 [12545165] Cancer Genet Cytogenet. 2003 Jan 15;140(2):113-7 [12645648] Int J Oncol. 2004 Sep;25(3):661-70 [15289867] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Histone deacetylase inhibitors and demethylating agents: clinical development of histone deacetylase inhibitors for cancer therapy. AN - 70430329; 17464244 AB - The histone deacetylase inhibitors are a new class of agents that are currently in various stages of clinical development. Clinical trials have demonstrated activity, urging further investigation. At the same time, it has been discovered that these agents have their own challenges. In this review, we discuss clinical data gathered to date, combination therapies designed to increase efficacy, and toxicities attributed to this new class of agents. JF - Cancer journal (Sudbury, Mass.) AU - Piekarz, Richard L AU - Sackett, Dan L AU - Bates, Susan E AD - Molecular Therapeutic Section, Medical Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1903, USA. rpiekarz@nih.gov PY - 2007 SP - 30 EP - 39 VL - 13 IS - 1 SN - 1528-9117, 1528-9117 KW - Antineoplastic Agents KW - 0 KW - Enzyme Inhibitors KW - Histone Deacetylase Inhibitors KW - Histone Deacetylases KW - EC 3.5.1.98 KW - Index Medicus KW - Animals KW - Humans KW - Clinical Trials as Topic KW - Histone Deacetylases -- chemistry KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use KW - Enzyme Inhibitors -- adverse effects KW - Enzyme Inhibitors -- therapeutic use KW - Enzyme Inhibitors -- chemistry KW - Antineoplastic Agents -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70430329?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+journal+%28Sudbury%2C+Mass.%29&rft.atitle=Histone+deacetylase+inhibitors+and+demethylating+agents%3A+clinical+development+of+histone+deacetylase+inhibitors+for+cancer+therapy.&rft.au=Piekarz%2C+Richard+L%3BSackett%2C+Dan+L%3BBates%2C+Susan+E&rft.aulast=Piekarz&rft.aufirst=Richard&rft.date=2007-01-01&rft.volume=13&rft.issue=1&rft.spage=30&rft.isbn=&rft.btitle=&rft.title=Cancer+journal+%28Sudbury%2C+Mass.%29&rft.issn=15289117&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-22 N1 - Date created - 2007-04-27 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Paclitaxel, bleomycin, etoposide, and cisplatin (T-BEP) as initial treatment in patients with poor-prognosis germ cell tumors (GCT): a phase II study. AN - 70409510; 17447857 AB - First line treatment of patients pts with poor-prognosis GCT, using BEP, is unsatisfactory. T-BEP (paclitaxel followed by BEP) demonstrated promising efficacy in the group of pts with intermediate and poor prognosis GCT. We present the results achieved with 1st line T-BEP in pts with poor-prognosis CGT. Twenty-four pts received T-BEP as initial therapy. Three pts (12.5%) had primary mediastinal GCT. Four cycles of T-BEP were given 21 days apart. Paclitaxel 175 mg/m2 was administered on day 1 before administration of BEP. The administration of G-CSF was not scheduled. Surgical resection of all radiographic residua was considered. All pts were assessable for response. Complete or partial response with negative tumor markers was achieved in 13 pts (54.2%; CI 95%: 34.3-74.1%). Median follow-up is 35.6 months. Median survival was not achieved and median time-to-progression is 9.5 months. Myelosuppression was the major toxicity with Gr3-4 granulocytopenia experienced in 52.1% of all courses. There were two treatment-related deaths due to sepsis. Patients treated with 1st line T-BEP didn't achieve higher response rate or time to progression. However, the overall survival observed in our study is surprisingly long. We do not recommend using this regimen without G-CSF support due to substantial toxicity. JF - Neoplasma AU - Mardiak, J AU - Sálek, T AU - Sycová-Milá, Z AU - Obertová, J AU - Recková, M AU - Mego, M AU - Hlavatá, Z AU - Brozmanová, K AU - Risnyovzská, Z AU - Svetlovská, D AU - Koza, I AD - National Cancer Institute, Bratislava, Slovakia. jozef.mardiak@nou.sk Y1 - 2007 PY - 2007 DA - 2007 SP - 240 EP - 245 VL - 54 IS - 3 SN - 0028-2685, 0028-2685 KW - Bleomycin KW - 11056-06-7 KW - Etoposide KW - 6PLQ3CP4P3 KW - Paclitaxel KW - P88XT4IS4D KW - Cisplatin KW - Q20Q21Q62J KW - Index Medicus KW - Paclitaxel -- administration & dosage KW - Neoplasm Staging KW - Bleomycin -- administration & dosage KW - Humans KW - Prognosis KW - Teratocarcinoma -- secondary KW - Cisplatin -- administration & dosage KW - Testicular Neoplasms -- drug therapy KW - Prospective Studies KW - Choriocarcinoma -- drug therapy KW - Etoposide -- administration & dosage KW - Carcinoma, Embryonal -- secondary KW - Adult KW - Teratocarcinoma -- drug therapy KW - Carcinoma, Embryonal -- drug therapy KW - Middle Aged KW - Choriocarcinoma -- secondary KW - Testicular Neoplasms -- secondary KW - Male KW - Neoplasms, Germ Cell and Embryonal -- pathology KW - Neoplasms, Germ Cell and Embryonal -- drug therapy KW - Antineoplastic Combined Chemotherapy Protocols -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70409510?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neoplasma&rft.atitle=Paclitaxel%2C+bleomycin%2C+etoposide%2C+and+cisplatin+%28T-BEP%29+as+initial+treatment+in+patients+with+poor-prognosis+germ+cell+tumors+%28GCT%29%3A+a+phase+II+study.&rft.au=Mardiak%2C+J%3BS%C3%A1lek%2C+T%3BSycov%C3%A1-Mil%C3%A1%2C+Z%3BObertov%C3%A1%2C+J%3BReckov%C3%A1%2C+M%3BMego%2C+M%3BHlavat%C3%A1%2C+Z%3BBrozmanov%C3%A1%2C+K%3BRisnyovzsk%C3%A1%2C+Z%3BSvetlovsk%C3%A1%2C+D%3BKoza%2C+I&rft.aulast=Mardiak&rft.aufirst=J&rft.date=2007-01-01&rft.volume=54&rft.issue=3&rft.spage=240&rft.isbn=&rft.btitle=&rft.title=Neoplasma&rft.issn=00282685&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-28 N1 - Date created - 2007-04-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Immuno-spin trapping analyses of DNA radicals. AN - 70347354; 17406615 AB - Immuno-spin trapping is a highly sensitive method for detecting DNA radicals in biological systems. This technique involves three main steps: (i) in situ and real-time trapping of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), thus forming DMPO-DNA nitrone adducts (referred to here as nitrone adducts); (ii) purification of nitrone adducts; and (iii) analysis of nitrone adducts by heterogeneous immunoassays using Abs against DMPO. In experiments, DMPO is added prior to the formation of free radicals. It diffuses easily through all cell compartments and is present when DNA free radicals are formed as a result of oxidative damage. Due to its low toxicity, DMPO can be used in cells at high enough concentrations to out-compete the normal reactions of DNA radicals, thus ensuring a high yield of DNA nitrone adducts. Because both protein and DNA nitrone adducts are formed, it is important that the DNA be pure in order to avoid misinterpretations. Depending on the model under study, this protocol can be completed in as few as 6 h. JF - Nature protocols AU - Ramirez, Dario C AU - Gomez-Mejiba, Sandra E AU - Mason, Ronald P AD - Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Building 101, MD F0-02, Research Triangle Park, North Carolina 27709, USA. ramirez1@niehs.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 512 EP - 522 VL - 2 IS - 3 KW - Cyclic N-Oxides KW - 0 KW - DNA Adducts KW - Free Radicals KW - 5,5-dimethyl-1-pyrroline-1-oxide KW - 7170JZ1QF3 KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Molecular Structure KW - DNA Adducts -- isolation & purification KW - Free Radicals -- isolation & purification KW - Cyclic N-Oxides -- metabolism KW - DNA Adducts -- metabolism KW - DNA -- isolation & purification KW - Immunoassay -- methods KW - DNA Damage KW - Spin Trapping -- methods KW - DNA -- metabolism KW - Spin Trapping -- instrumentation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70347354?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+protocols&rft.atitle=Immuno-spin+trapping+analyses+of+DNA+radicals.&rft.au=Ramirez%2C+Dario+C%3BGomez-Mejiba%2C+Sandra+E%3BMason%2C+Ronald+P&rft.aulast=Ramirez&rft.aufirst=Dario&rft.date=2007-01-01&rft.volume=2&rft.issue=3&rft.spage=512&rft.isbn=&rft.btitle=&rft.title=Nature+protocols&rft.issn=1750-2799&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-28 N1 - Date created - 2007-04-04 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Chem Res Toxicol. 2000 Oct;13(10):1056-64 [11080055] Free Radic Biol Med. 2003 Apr 15;34(8):1089-99 [12684094] Free Radic Biol Med. 2002 Aug 1;33(3):364-9 [12126758] Free Radic Biol Med. 2003 Jun 1;34(11):1473-81 [12757857] Arch Biochem Biophys. 2003 Jul 15;415(2):251-6 [12831849] FASEB J. 2003 Jul;17(10):1195-214 [12832285] Mutat Res. 2003 Oct 29;531(1-2):5-23 [14637244] Arch Biochem Biophys. 2004 Mar 1;423(1):57-65 [14989265] Free Radic Biol Med. 2004 May 15;36(10):1214-23 [15110386] Br J Pharmacol. 2004 May;142(2):231-55 [15155533] Nature. 1987 Dec 24-31;330(6150):773-4 [2827034] Science. 1988 Apr 29;240(4852):640-2 [2834821] Proc Natl Acad Sci U S A. 1990 Jun;87(12):4533-7 [2352934] Free Radic Res Commun. 1990;11(4-5):195-206 [1965722] Basic Life Sci. 1991;58:287-317; discussion 317-21 [1811474] Biochemistry. 1993 Oct 12;32(40):10599-606 [8399204] FEBS Lett. 1995 Oct 16;373(3):299-302 [7589487] Nature. 1996 Aug 22;382(6593):731-5 [8751447] J Biochem Biophys Methods. 1996 Jul 10;32(3):183-90 [8844325] Free Radic Biol Med. 1996;21(4):427-36 [8886792] FEBS Lett. 1997 Nov 24;418(1-2):73-5 [9414098] Mutat Res. 1999 Jul 16;428(1-2):17-22 [10517974] Trends Biochem Sci. 2005 Aug;30(8):453-61 [15996871] Nat Methods. 2006 Feb;3(2):123-7 [16432522] J Phys Chem B. 2005 Sep 8;109(35):16967-73 [16853159] Rapid Commun Mass Spectrom. 2006;20(15):2235-42 [16810703] Free Radic Biol Med. 2003 Apr 1;34(7):830-9 [12654471] Physiol Rev. 2002 Jan;82(1):47-95 [11773609] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Differential gene expression patterns in cyclooxygenase-1 and cyclooxygenase-2 deficient mouse brain. AN - 70314951; 17266762 AB - Cyclooxygenase (COX)-1 and COX-2 produce prostanoids from arachidonic acid and are thought to have important yet distinct roles in normal brain function. Deletion of COX-1 or COX-2 results in profound differences both in brain levels of prostaglandin E2 and in activation of the transcription factor nuclear factor-kappaB, suggesting that COX-1 and COX-2 play distinct roles in brain arachidonic acid metabolism and regulation of gene expression. To further elucidate the role of COX isoforms in the regulation of the brain transcriptome, microarray analysis of gene expression in the cerebral cortex and hippocampus of mice deficient in COX-1 (COX-1-/-) or COX-2 (COX-2-/-) was performed. A majority (>93%) of the differentially expressed genes in both the cortex and hippocampus were altered in one COX isoform knockout mouse but not the other. The major gene function affected in all genotype comparisons was 'transcriptional regulation'. Distinct biologic and metabolic pathways that were altered in COX-/- mice included beta oxidation, methionine metabolism, janus kinase signaling, and GABAergic neurotransmission. Our findings suggest that COX-1 and COX-2 differentially modulate brain gene expression. Because certain anti-inflammatory and analgesic treatments are based on inhibition of COX activity, the specific alterations observed in this study further our understanding of the relationship of COX-1 and COX-2 with signaling pathways in brain and of the therapeutic and toxicologic consequences of COX inhibition. JF - Genome biology AU - Toscano, Christopher D AU - Prabhu, Vinaykumar V AU - Langenbach, Robert AU - Becker, Kevin G AU - Bosetti, Francesca AD - Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, Maryland 20892, USA. toscanoc@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1 VL - 8 IS - 1 KW - GABA Plasma Membrane Transport Proteins KW - 0 KW - Receptors, GABA KW - Methionine KW - AE28F7PNPL KW - Cyclooxygenase 1 KW - EC 1.14.99.1 KW - Cyclooxygenase 2 KW - Janus Kinases KW - EC 2.7.10.2 KW - Index Medicus KW - Animals KW - GABA Plasma Membrane Transport Proteins -- metabolism KW - Janus Kinases -- metabolism KW - Methionine -- metabolism KW - Up-Regulation -- genetics KW - Mice KW - Receptors, GABA -- genetics KW - Mice, Knockout KW - Genotype KW - Oxidation-Reduction KW - GABA Plasma Membrane Transport Proteins -- genetics KW - Receptors, GABA -- metabolism KW - Janus Kinases -- genetics KW - Gene Expression Profiling KW - Cyclooxygenase 2 -- deficiency KW - Cerebral Cortex -- enzymology KW - Hippocampus -- enzymology KW - Cyclooxygenase 1 -- deficiency KW - Gene Expression Regulation -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70314951?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Genome+biology&rft.atitle=Differential+gene+expression+patterns+in+cyclooxygenase-1+and+cyclooxygenase-2+deficient+mouse+brain.&rft.au=Toscano%2C+Christopher+D%3BPrabhu%2C+Vinaykumar+V%3BLangenbach%2C+Robert%3BBecker%2C+Kevin+G%3BBosetti%2C+Francesca&rft.aulast=Toscano&rft.aufirst=Christopher&rft.date=2007-01-01&rft.volume=8&rft.issue=1&rft.spage=R14&rft.isbn=&rft.btitle=&rft.title=Genome+biology&rft.issn=1474-760X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-13 N1 - Date created - 2007-03-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Exp Cell Res. 2005 May 15;306(1):75-84 [15878334] Neuroscience. 1999;92(3):1061-77 [10426546] Brain Res Mol Brain Res. 2005 Oct 3;139(2):217-24 [16055227] FEBS J. 2005 Oct;272(19):4874-83 [16176262] J Clin Immunol. 2006 Jan;26(1):73-85 [16418805] J Neurochem. 2006 Feb;96(3):669-79 [16405503] J Immunol. 2006 Mar 15;176(6):3774-9 [16517747] Acta Neuropathol. 2006 Apr;111(4):351-63 [16456667] Brain Res Brain Res Protoc. 1999 Dec;4(3):341-50 [10592344] Ann N Y Acad Sci. 1999;889:52-61 [10668482] J Neurochem. 2000 Mar;74(3):1041-8 [10693935] Prostaglandins Other Lipid Mediat. 2004 Oct;74(1-4):1-10 [15560112] J Neurochem. 2004 Dec;91(6):1389-97 [15584915] J Neurochem. 2006 Aug;98(3):801-11 [16787416] Adv Exp Med Biol. 1999;466:133-43 [10709637] Anaesthesia. 2000 May;55(5):442-9 [10792135] Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8600-5 [10900018] Annu Rev Biochem. 2000;69:145-82 [10966456] J Neurosci. 2000 Sep 15;20(18):6920-6 [10995836] Inflamm Res. 2000 Aug;49(8):367-92 [11028754] Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1294-9 [11158633] Cell Res. 2001 Mar;11(1):61-7 [11305326] Cell Biochem Biophys. 2000;33(2):101-25 [11325033] J Biol Chem. 2001 Jul 6;276(27):24918-24 [11337507] Methods. 2001 Dec;25(4):402-8 [11846609] J Mol Diagn. 2003 May;5(2):73-81 [12707371] Neuroreport. 2003 Oct 27;14(15):1927-9 [14561922] Prog Neuropsychopharmacol Biol Psychiatry. 2004 May;28(3):453-64 [15093951] J Neurochem. 1983 Sep;41(3):607-10 [6875555] FEBS Lett. 1989 Nov 6;257(2):377-9 [2479580] J Neurochem. 1994 Mar;62(3):1144-53 [8113801] J Biol Chem. 1995 May 5;270(18):10902-8 [7738031] Brain Res Mol Brain Res. 1996 Apr;37(1-2):309-16 [8738166] Brain Res Mol Brain Res. 1997 May;45(2):268-74 [9149101] Pharmacol Ther. 1997;73(3):265-80 [9175157] Annu Rev Immunol. 1998;16:293-322 [9597132] Biochem J. 1998 Aug 15;334 ( Pt 1):113-9 [9693110] Nat Med. 1998 Oct;4(10):1166-72 [9771750] Life Sci. 1999;64(24):2173-86 [10374907] Prostaglandins Other Lipid Mediat. 2005 Sep;77(1-4):185-96 [16099403] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ethnic differences among adolescents seeking smoking cessation treatment: a structural analysis of responses on the Fagerström Test for Nicotine Dependence. AN - 70279172; 17365744 AB - Features of tobacco dependence vary by ethnicity, which could be partially due to measurement bias inherent in instruments that assess nicotine dependence. This study compared responses on the Fagerström Test for Nicotine Dependence (FTND) by African American adolescents (n = 478) with those of White adolescents (n = 661) seeking smoking cessation treatment. We conducted item-by-item comparisons by ethnicity for the six questions composing the FTND and confirmatory factor analyses and multiple indicators-multiple causes (MIMIC) modeling to test the hypothesis of measurement invariance of the FTND by ethnicity. Study participants (N = 1,139) were daily smokers of average age 15.4 years (SD = 1.3); 42.0% were African American and 61.5% female. White adolescents' pattern of responses to the FTND indicated a greater degree of dependence; these differences were statistically significant for five of the six items. The FTND exhibited a unidimensional structure with similar factor loadings in both White and African American adolescents. However, MIMIC modeling indicated differential reporting for three out of six items, suggesting that the FTND may not measure nicotine dependence equivalently for White and African American youth. JF - Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco AU - Schroeder, Jennifer R AU - Moolchan, Eric T AD - Intramural Research Program, National Institute on Drug Abuse, Baltimore, MD 21224, USA. jschroed@intra.nida.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 137 EP - 145 VL - 9 IS - 1 SN - 1462-2203, 1462-2203 KW - Index Medicus KW - Demography KW - Factor Analysis, Statistical KW - Humans KW - Surveys and Questionnaires KW - Adolescent KW - Male KW - Female KW - Patient Acceptance of Health Care -- statistics & numerical data KW - Smoking Cessation -- methods KW - Tobacco Use Disorder -- ethnology KW - Tobacco Use Disorder -- prevention & control KW - Smoking -- ethnology KW - Smoking -- prevention & control KW - Tobacco Use Disorder -- diagnosis KW - Ethnic Groups -- statistics & numerical data UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70279172?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nicotine+%26+tobacco+research+%3A+official+journal+of+the+Society+for+Research+on+Nicotine+and+Tobacco&rft.atitle=Ethnic+differences+among+adolescents+seeking+smoking+cessation+treatment%3A+a+structural+analysis+of+responses+on+the+Fagerstr%C3%B6m+Test+for+Nicotine+Dependence.&rft.au=Schroeder%2C+Jennifer+R%3BMoolchan%2C+Eric+T&rft.aulast=Schroeder&rft.aufirst=Jennifer&rft.date=2007-01-01&rft.volume=9&rft.issue=1&rft.spage=137&rft.isbn=&rft.btitle=&rft.title=Nicotine+%26+tobacco+research+%3A+official+journal+of+the+Society+for+Research+on+Nicotine+and+Tobacco&rft.issn=14622203&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-05-08 N1 - Date created - 2007-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nicotine, cotinine, withdrawal, and craving patterns during smoking and nicotine nasal spray use: results from a pilot study with African American men. AN - 70276439; 17365738 AB - Nicotine intake via smoking is highly variable. Individualized dosing of nicotine replacement therapy (NRT) may improve product efficacy, but a better understanding of the within-day and within-subject relationships between smoking, NRT use, nicotine and cotinine concentrations in blood, and cravings and withdrawal symptoms is needed to inform dosing algorithms. A pilot study was undertaken to collect data on these relationships and to assess the feasibility of the methods needed for this type of research, including a sophisticated statistical modeling technique (a two-part mixed-effects model with correlated random effects that accounts for clumping at zero). Because nicotine metabolism varies by gender and race, the sample was homogeneous with respect to these characteristics. In a within-subjects study, 27 African American adult male smokers carried a computerized cigarette dispenser for 1 week, capturing the time each cigarette was smoked. Subjects then entered an inpatient setting for 1 day of scheduled smoking (matched to data from the cigarette dispenser to create an ecologically valid schedule) and 4 days of ad libitum nicotine nasal spray use, while tobacco abstinent. Eight times per day, at 2-hour intervals, blood was drawn and ratings of cigarette cravings and withdrawal symptoms were obtained. On average, subjects used less than half of the manufacturer's recommended minimum daily dose of nicotine nasal spray. Large differences in nicotine and cotinine levels were observed between individuals. When predicting nicotine, cotinine, withdrawal, and cravings, we observed significant interactions between route of nicotine intake and a variety of independent variables. JF - Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco AU - Mabry, Patricia L AU - Tooze, Janet A AU - Moser, Richard P AU - Augustson, Erik M AU - Malcolm, Robert J AU - Benowitz, Neal L AD - SAIC-Frederick, Inc., support to Tobacco Control Research Branch (TCRB), Behavioral Research Program (BRP), Division of Cancer Control and Population Sciences (DCCPS), National Cancer Institute. Bethesda, MD. mabryp@od.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 65 EP - 82 VL - 9 IS - 1 SN - 1462-2203, 1462-2203 KW - Ganglionic Stimulants KW - 0 KW - Nicotine KW - 6M3C89ZY6R KW - Cotinine KW - K5161X06LL KW - Index Medicus KW - Drug Administration Schedule KW - Humans KW - Administration, Intranasal KW - Adult KW - Smoking Cessation -- methods KW - Algorithms KW - Pilot Projects KW - Male KW - Nicotine -- therapeutic use KW - Tobacco Use Disorder -- blood KW - African Americans -- statistics & numerical data KW - Nicotine -- adverse effects KW - Cotinine -- blood KW - Ganglionic Stimulants -- therapeutic use KW - Tobacco Use Disorder -- ethnology KW - Nicotine -- administration & dosage KW - Smoking -- prevention & control KW - Disruptive, Impulse Control, and Conduct Disorders -- epidemiology KW - Substance Withdrawal Syndrome -- etiology KW - Ganglionic Stimulants -- adverse effects KW - Substance Withdrawal Syndrome -- epidemiology KW - Tobacco Use Disorder -- prevention & control KW - Smoking -- ethnology KW - Ganglionic Stimulants -- administration & dosage UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70276439?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nicotine+%26+tobacco+research+%3A+official+journal+of+the+Society+for+Research+on+Nicotine+and+Tobacco&rft.atitle=Nicotine%2C+cotinine%2C+withdrawal%2C+and+craving+patterns+during+smoking+and+nicotine+nasal+spray+use%3A+results+from+a+pilot+study+with+African+American+men.&rft.au=Mabry%2C+Patricia+L%3BTooze%2C+Janet+A%3BMoser%2C+Richard+P%3BAugustson%2C+Erik+M%3BMalcolm%2C+Robert+J%3BBenowitz%2C+Neal+L&rft.aulast=Mabry&rft.aufirst=Patricia&rft.date=2007-01-01&rft.volume=9&rft.issue=1&rft.spage=65&rft.isbn=&rft.btitle=&rft.title=Nicotine+%26+tobacco+research+%3A+official+journal+of+the+Society+for+Research+on+Nicotine+and+Tobacco&rft.issn=14622203&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-05-08 N1 - Date created - 2007-03-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The new EPA regulations for protecting human subjects: haste makes waste. AN - 70248237; 17348257 JF - The Hastings Center report AU - Resnik, David B AD - National Institute of Health Sciences, National Institutes of Health, USA. PY - 2007 SP - 17 EP - 21 VL - 37 IS - 1 SN - 0093-0334, 0093-0334 KW - Environmental Pollutants KW - 0 KW - Pesticides KW - Bioethics KW - Index Medicus KW - United States KW - Government Regulation KW - Humans KW - United States Dept. of Health and Human Services KW - Chemical Industry KW - Advisory Committees KW - Research Subjects -- legislation & jurisprudence KW - United States Environmental Protection Agency KW - Environmental Exposure -- adverse effects KW - Decision Making KW - Food -- standards KW - Pesticides -- adverse effects KW - Environmental Pollutants -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70248237?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Hastings+Center+report&rft.atitle=The+new+EPA+regulations+for+protecting+human+subjects%3A+haste+makes+waste.&rft.au=Resnik%2C+David+B&rft.aulast=Resnik&rft.aufirst=David&rft.date=2007-01-01&rft.volume=37&rft.issue=1&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=The+Hastings+Center+report&rft.issn=00930334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-05-30 N1 - Date created - 2007-03-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Science. 2005 Jul 8;309(5732):232 [16002591] Account Res. 2005 Apr-Jun;12(2):69-101 [16220621] Environ Health Perspect. 2005 Jul;113(7):813-7 [16002367] Environ Health Perspect. 2004 Jun;112(8):914-9 [15175182] Kennedy Inst Ethics J. 1997 Sep;7(3):291-8 [11660360] Am J Public Health. 2004 Nov;94(11):1908-16 [15514226] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Imaging techniques for small animal imaging models of pulmonary disease: micro-CT. AN - 70225062; 17325973 AB - Microcomputed tomography (micro-CT) is ideal for quantifying pulmonary disease because of the inherent contrast between tissue and air that exists in the lungs. Both in vivo and in vitro studies can be performed using micro-CT. Live animal studies show function, while fixed specimen studies show structure. Through the use of image processing techniques, both acute and chronic lung diseases can be quantified. The information provided by micro-CT is complementary to histological evaluation, since CT is nondestructive. This paper discusses two examples, in vivo and in vitro, of how micro-CT can be used to assess pulmonary diseases in small animal models. With the use of micro-CT, we were able to quantify pulmonary fibrosis in the live rat and investigate the microstructure of the airway in fixed mouse lungs. JF - Toxicologic pathology AU - Johnson, Kennita A AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. johnso58@niehs.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 59 EP - 64 VL - 35 IS - 1 SN - 0192-6233, 0192-6233 KW - Antibiotics, Antineoplastic KW - 0 KW - Bleomycin KW - 11056-06-7 KW - Index Medicus KW - Acute Disease KW - Animals KW - Microcomputers KW - Gene Silencing KW - Bleomycin -- toxicity KW - Mice KW - Antibiotics, Antineoplastic -- toxicity KW - Mice, Knockout KW - Rats KW - Bronchi -- anatomy & histology KW - Imaging, Three-Dimensional KW - Rats, Inbred F344 KW - Mice, Inbred C57BL KW - Chronic Disease KW - Female KW - Male KW - Bronchography KW - Tomography, X-Ray Computed -- methods KW - Lung -- diagnostic imaging KW - Pulmonary Fibrosis -- pathology KW - Pulmonary Fibrosis -- diagnostic imaging KW - Pulmonary Fibrosis -- chemically induced KW - Lung -- drug effects KW - Disease Models, Animal KW - Lung -- pathology KW - Image Processing, Computer-Assisted UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70225062?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+pathology&rft.atitle=Imaging+techniques+for+small+animal+imaging+models+of+pulmonary+disease%3A+micro-CT.&rft.au=Johnson%2C+Kennita+A&rft.aulast=Johnson&rft.aufirst=Kennita&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=59&rft.isbn=&rft.btitle=&rft.title=Toxicologic+pathology&rft.issn=01926233&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-17 N1 - Date created - 2007-02-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Cell Biochem Suppl. 2002;39:116-24 [12552611] J Biomech. 2003 Nov;36(11):1587-94 [14522199] Acad Radiol. 2003 Oct;10(10):1104-18 [14587629] Med Phys. 2003 Nov;30(11):2869-77 [14655933] Phys Med Biol. 2005 Apr 7;50(7):1405-19 [15798332] Mol Imaging. 2004 Jan;3(1):55-62 [15142412] Phys Med Biol. 2004 Sep 7;49(17):4163-72 [15470930] Am J Respir Crit Care Med. 1998 Nov;158(5 Pt 1):1571-7 [9817710] Med Phys. 2004 Dec;31(12):3324-9 [15651615] Am J Respir Cell Mol Biol. 2004 Feb;30(2):129-38 [14729505] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - K-ras mutations in lung tumors and tumors from other organs are consistent with a common mechanism of ethylene oxide tumorigenesis in the B6C3F1 mouse. AN - 70219074; 17325976 AB - Ethylene oxide is a multisite carcinogen in rodents and classified as a human carcinogen by the National Toxicology Program. In 2-year mouse studies, ethylene oxide (EO) induced lung, Harderian gland (HG), and uterine neoplasms. We evaluated representative EO-induced and equivalent spontaneous neoplasms for K-ras mutations in codons 12, 13, and 61. K-ras mutations were identified in 100% (23/23) of the EO-induced lung neoplasms and 25% (27/108) of the spontaneous lung neoplasms. Codon 12 G to T transversions were common in EO-induced lung neoplasms (21/23) but infrequent in spontaneous lung neoplasms (1/108). K-ras mutations were found in 86% (18/21) of the EO-induced HG neoplasms and 7% (2/27) of the spontaneous HG neoplasms. Codon 13 G to C and codon 12 G to T transversions were predominant in the EO-induced HG neoplasms but absent in spontaneous HG neoplasms (0/27). K-ras mutations occurred in 83% (5/6) of the EO-induced uterine carcinomas and all were codon 13 C to T transitions. These data show a strong predilection for development of K-ras mutations in EO-induced lung, Harderian gland, and uterine neoplasms. This suggests that EO specifically targets the K-ras gene in multiple tissue types and that this event is a critical component of EO-induced tumorigenesis. JF - Toxicologic pathology AU - Hong, Hue-Hua L AU - Houle, Christopher D AU - Ton, Thai-Vu T AU - Sills, Robert C AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA. hong5@niehs.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 81 EP - 85 VL - 35 IS - 1 SN - 0192-6233, 0192-6233 KW - Carcinogens KW - 0 KW - DNA, Neoplasm KW - Disinfectants KW - Ethylene Oxide KW - JJH7GNN18P KW - Index Medicus KW - Mice, Inbred Strains KW - Animals KW - Harderian Gland -- pathology KW - Dose-Response Relationship, Drug KW - Inhalation Exposure KW - Mice KW - DNA, Neoplasm -- analysis KW - Harderian Gland -- drug effects KW - Mutation KW - Disinfectants -- toxicity KW - Male KW - Female KW - Uterine Neoplasms -- genetics KW - Adenocarcinoma -- chemically induced KW - Uterine Neoplasms -- chemically induced KW - Carcinogens -- toxicity KW - Adenocarcinoma -- genetics KW - Uterine Neoplasms -- pathology KW - Adenocarcinoma -- pathology KW - Genes, ras KW - Adenoma -- chemically induced KW - Lung Neoplasms -- genetics KW - Sebaceous Gland Neoplasms -- pathology KW - Lung Neoplasms -- chemically induced KW - Sebaceous Gland Neoplasms -- genetics KW - Adenoma -- genetics KW - Adenoma -- pathology KW - Sebaceous Gland Neoplasms -- chemically induced KW - Ethylene Oxide -- toxicity KW - Lung Neoplasms -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70219074?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+pathology&rft.atitle=K-ras+mutations+in+lung+tumors+and+tumors+from+other+organs+are+consistent+with+a+common+mechanism+of+ethylene+oxide+tumorigenesis+in+the+B6C3F1+mouse.&rft.au=Hong%2C+Hue-Hua+L%3BHoule%2C+Christopher+D%3BTon%2C+Thai-Vu+T%3BSills%2C+Robert+C&rft.aulast=Hong&rft.aufirst=Hue-Hua&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=81&rft.isbn=&rft.btitle=&rft.title=Toxicologic+pathology&rft.issn=01926233&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-17 N1 - Date created - 2007-02-27 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Carcinogenesis. 1991 Feb;12(2):299-303 [1995195] N Engl J Med. 1991 May 16;324(20):1402-7 [2020295] Mutat Res. 1991 Sep-Oct;250(1-2):483-97 [1719390] Mutat Res. 1992 Jan;281(1):31-7 [1371589] Cancer Res. 1992 Aug 15;52(16):4328-34 [1643630] Chem Biol Interact. 1992 Jun 15;83(1):35-54 [1643667] Carcinogenesis. 1993 May;14(5):795-801 [8504471] Mutat Res. 1994 Jan 16;304(2):229-34 [7506366] Br J Ind Med. 1993 Nov;50(11):971-97 [8280635] Carcinogenesis. 1994 Nov;15(11):2665-7 [7955123] Environ Mol Mutagen. 1994;24(3):161-7 [7957119] IARC Monogr Eval Carcinog Risks Hum. 1994;60:1-560 [7869568] Cancer Lett. 1995 Jun 8;92(2):223-7 [7600534] Carcinogenesis. 1995 Jul;16(7):1623-8 [7614698] Jpn J Cancer Res. 1995 Sep;86(9):802-10 [7591956] Toxicol Appl Pharmacol. 1996 Jan;136(1):8-19 [8560484] Carcinogenesis. 1997 Apr;18(4):783-9 [9111215] Mutat Res. 1997 Jul 14;391(3):153-64 [9268040] Br J Cancer. 1998 Mar;77(5):720-3 [9514049] Eur J Cancer Prev. 1998 Apr;7(2):167-8 [9818781] Carcinogenesis. 1999 Apr;20(4):657-62 [10223196] Carcinogenesis. 1999 Sep;20(9):1787-92 [10469625] Mutat Res. 1999 Dec 17;431(2):397-415 [10636004] Mutat Res. 2000 Jan 17;447(1):27-48 [10686305] Carcinogenesis. 2000 Sep;21(9):1661-9 [10964097] Cancer Lett. 2001 Mar 26;164(2):207-12 [11179836] Mutat Res. 2001 May 31;492(1-2):59-67 [11377244] Toxicol Pathol. 2001 Jul-Aug;29(4):422-9 [11560247] Ann N Y Acad Sci. 2002 Dec;982:177-89 [12562636] Mutagenesis. 2004 May;19(3):215-22 [15123787] Pharmacol Rev. 1966 Mar;18(1):805-38 [5325210] Carcinogenesis. 1990 Feb;11(2):307-12 [2302758] Mol Carcinog. 1990;3(5):287-95 [2244961] Int J Oncol. 2005 May;26(5):1241-55 [15809715] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Harmonization of internal dosimetry procedures in Latin America--ARCAL/IAEA project. AN - 70134617; 17569691 AB - Under the auspices of the Regional Coordination Agreement for Latin America, representatives of the eight member states have participated in a project to improve radiological protection for workers exposed to unsealed sources of radiation. The design of the project was based on information obtained from a questionnaire circulated among the participants, from which the initial status of internal dosimetry services in each country was characterised. The objective of the project is to harmonize internal dosimetry procedures, with reference to International Atomic Energy Agency recommendations. After the implementation of new procedures and personnel training, four intercomparison exercises were carried out: measurement of iodine in thyroid phantoms, measurement of gamma emitters in urine samples, measurement of beta emitters in urine samples and internal dose assessments. This project has resulted in important improvements in internal dosimetry services in the region. JF - Radiation protection dosimetry AU - Melo, D AU - Suarez, R Cruz AU - Rojo, A AU - Dantas, B M AU - Julião, L AU - Serdero, N AU - Videla, R AU - Puerta, J A AU - Lopez, G AU - Alfaro, M M AU - Gonzáles, S AU - Hermida, J C AU - Navarro, T AD - Instituto de Radioproteção e Dosimetria - Av. Salvador Allende S/N, Recreio dos Bandeirantes, RJ 22780-160, Brazil. melodun@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 325 EP - 328 VL - 127 IS - 1-4 SN - 0144-8420, 0144-8420 KW - Index Medicus KW - Radiation Dosage KW - Latin America KW - Biological Assay -- methods KW - Radiation Protection -- methods KW - Safety Management -- methods KW - Interinstitutional Relations KW - Radiometry -- methods KW - Safety Management -- organization & administration UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70134617?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Radiation+protection+dosimetry&rft.atitle=Harmonization+of+internal+dosimetry+procedures+in+Latin+America--ARCAL%2FIAEA+project.&rft.au=Melo%2C+D%3BSuarez%2C+R+Cruz%3BRojo%2C+A%3BDantas%2C+B+M%3BJuli%C3%A3o%2C+L%3BSerdero%2C+N%3BVidela%2C+R%3BPuerta%2C+J+A%3BLopez%2C+G%3BAlfaro%2C+M+M%3BGonz%C3%A1les%2C+S%3BHermida%2C+J+C%3BNavarro%2C+T&rft.aulast=Melo&rft.aufirst=D&rft.date=2007-01-01&rft.volume=127&rft.issue=1-4&rft.spage=325&rft.isbn=&rft.btitle=&rft.title=Radiation+protection+dosimetry&rft.issn=01448420&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-08-19 N1 - Date created - 2008-05-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Environmental exposure and children's health in China. AN - 70106182; 18316263 AB - Concerns regarding adverse health effects of exposure to environmental pollutants among children are increasing. This subject area is particularly important in China because of environmental pollution problems associated with rapid development and a large population. The authors provide what is, to their knowledge, the first review in China of English- and Chinese-language literature regarding the current status of environmental exposures to children in China and the impact of these exposures on the health of children. Children in China are exposed to diverse environmental pollutants, including traditional pollutants such as lead and mercury and emerging pollutants such as phthalates and perfluorinated compounds. Incidence and prevalence of certain childhood diseases have increased in last decades. In China, a limited number of data on environmental exposure and children's health are available, and further high-quality studies are needed. JF - Archives of environmental & occupational health AU - Ye, Xibiao AU - Fu, Hua AU - Guidotti, Tee AD - Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Research Triangle Park, North Carolina 27709, USA. yex2@niehs.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 61 EP - 73 VL - 62 IS - 2 SN - 1933-8244, 1933-8244 KW - Environmental Pollutants KW - 0 KW - Hazardous Substances KW - Methylmercury Compounds KW - Organophosphates KW - Lead KW - 2P299V784P KW - Arsenic KW - N712M78A8G KW - Abridged Index Medicus KW - Index Medicus KW - Arsenic -- toxicity KW - Humans KW - Infant, Newborn KW - Child KW - Methylmercury Compounds -- toxicity KW - Child, Preschool KW - Infant KW - Lead -- toxicity KW - Organophosphates -- toxicity KW - Adolescent KW - China KW - Female KW - Male KW - Environmental Pollutants -- toxicity KW - Environmental Health KW - Environmental Exposure -- adverse effects KW - Child Welfare KW - Hazardous Substances -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70106182?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Archives+of+environmental+%26+occupational+health&rft.atitle=Environmental+exposure+and+children%27s+health+in+China.&rft.au=Ye%2C+Xibiao%3BFu%2C+Hua%3BGuidotti%2C+Tee&rft.aulast=Ye&rft.aufirst=Xibiao&rft.date=2007-01-01&rft.volume=62&rft.issue=2&rft.spage=61&rft.isbn=&rft.btitle=&rft.title=Archives+of+environmental+%26+occupational+health&rft.issn=19338244&rft_id=info:doi/10.3200%2FAEOH.62.2.61-73 LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-04-30 N1 - Date created - 2008-03-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 DO - http://dx.doi.org/10.3200/AEOH.62.2.61-73 ER - TY - JOUR T1 - Clinical significance of metabolic superscan in patients with hyperthyroidism. AN - 70065608; 18228210 AB - Hyperthyroid patients commonly complain of generalized bony aches, which are frequently overlooked due to the more prominent symptoms of cardiovascular and nervous disturbances. Hyperthyroid patients are expected to have abnormal bone metabolism as part of the generalized hypermetabolic status. The aim of this study is to verify the presence of metabolic bone superscan in association with the hypermetabolic stats in various groups of hyperthyroidism. Secondly, to correlate these superscan features with the various laboratory results in hyperthyroid patients. Forty-five hyperthyroid patients confirmed by clinical and laboratory results were enrolled in this work. In all patients, a (99m)Tc-pertechnetate thyroid uptake scan was acquired. On a different day, total body bone scan was acquired three hours post IV injection of 555-925 MBq of (99m)Tc-MDP. Serum FT3, FT4, TSH, Ca++, alkaline phosphatase (AP) and parathyroid hormone (PTH) were monitored in all patients as markers of thyroid and bone metabolism. Ten cases with no thyroid diseases were included as a control group. Patients with thyroiditis or long history of antithyroid drugs for more than one year were excluded from the study. The patients were subdivided into three groups: Graves disease (GD) (n = 30), toxic nodular goiter (TNG) (n = 10) and autonomous toxic adenoma (AT) (n = 5). The TSH for the whole group was significantly suppressed compared to the control group with higher suppression in the Graves disease group than in the TNG or AT groups. (99m)Tc-pertechnetate uptake values in the Graves disease group were significantly higher than the TNG and AT groups (p 0.05). Disturbances in bone metabolism are more prevalent in Graves disease than in other types of hyperthyroidism. The addition of the bone scan to the diagnostic work up of patients with Graves disease is a sensitive indicator for metabolic bone changes and could help in the future management and follow up for this group of patients. JF - Nuclear medicine review. Central & Eastern Europe AU - Kotb, Magdy H AU - El-Maghraby, Tarek AU - Khalafallah, Khaled AU - Omar, Walid AU - Grace, Bahaa Demian AU - Al-Nahhas, Adil AD - Nuclear Medicine, National Cancer Institute, Cairo University, Egypt. Y1 - 2007 PY - 2007 DA - 2007 SP - 76 EP - 81 VL - 10 IS - 2 SN - 1506-9680, 1506-9680 KW - Radiopharmaceuticals KW - 0 KW - Sodium Pertechnetate Tc 99m KW - A0730CX801 KW - Technetium Tc 99m Medronate KW - X89XV46R07 KW - Index Medicus KW - Sensitivity and Specificity KW - Reproducibility of Results KW - Humans KW - Adult KW - Male KW - Female KW - Radionuclide Imaging KW - Bone Diseases, Metabolic -- diagnostic imaging KW - Hyperthyroidism -- diagnostic imaging KW - Bone Diseases, Metabolic -- complications KW - Hyperthyroidism -- complications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70065608?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nuclear+medicine+review.+Central+%26+Eastern+Europe&rft.atitle=Clinical+significance+of+metabolic+superscan+in+patients+with+hyperthyroidism.&rft.au=Kotb%2C+Magdy+H%3BEl-Maghraby%2C+Tarek%3BKhalafallah%2C+Khaled%3BOmar%2C+Walid%3BGrace%2C+Bahaa+Demian%3BAl-Nahhas%2C+Adil&rft.aulast=Kotb&rft.aufirst=Magdy&rft.date=2007-01-01&rft.volume=10&rft.issue=2&rft.spage=76&rft.isbn=&rft.btitle=&rft.title=Nuclear+medicine+review.+Central+%26+Eastern+Europe&rft.issn=15069680&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-05-14 N1 - Date created - 2008-01-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In vitro mitigation of arsenic toxicity by tea polyphenols in human lymphocytes. AN - 70064155; 18197836 AB - The groundwater arsenicals have brought dreadful misery for the people residing in the endemic regions of West Bengal, India. Arsenic-related anomalies include arsenicosis, hyperkera-tosis, gastric complications, liver fibrosis, peripheral neuropathy, and cancer. Some of these diseases have been frequently associated with overproduction of reactive oxygen species that cause DNA damage and improper functioning of body's antioxidant defense mechanism. Natural polyphenols present in tea serve as excellent antioxidants. In the present study, an attempt has been made to elucidate the role of representative polyphenols and extracts of green and black tea in modulating sodium arsenite (As III)-induced DNA damage in normal human lymphocytes. Comet assay was used to detect the DNA damage. Arsenic-induced oxidative stress was measured with generation of reactive oxygen species, lipid peroxidation, and activity of some antioxidant enzymes. Expression of some repair enzymes such as poly(ADP-ribose) polymerase and DNA polymerase beta was measured to assess the effect of tea on DNA repair. Tea afforded efficient reduction of As III-induced DNA damage in human lymphocytes. Tea also quenched the excessive production of reactive oxygen species by arsenic, reduced the elevated levels of lipid peroxidation, and increased the activity of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase. Furthermore, tea enhanced recovery of DNA damage, which was indicative of repair as confirmed by unscheduled DNA synthesis and pronounced expression of DNA repair enzyme poly(ADP-ribose) polymerase. It is speculated that the antioxidant potential and repair-inducing capacity of tea might help in combating the severe genotoxic effects induced by arsenic in the human population. JF - Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer AU - Sinha, Dona AU - Dey, Subhabrata AU - Bhattacharya, Rathindra Kumar AU - Roy, Madhumita AD - Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, India. Y1 - 2007 PY - 2007 DA - 2007 SP - 207 EP - 220 VL - 26 IS - 3 SN - 0731-8898, 0731-8898 KW - Antioxidants KW - 0 KW - Flavonoids KW - Phenols KW - Plant Extracts KW - Polyphenols KW - Reactive Oxygen Species KW - Tea KW - Arsenic KW - N712M78A8G KW - Index Medicus KW - Comet Assay KW - Camellia sinensis -- chemistry KW - Phenols -- pharmacology KW - Cells, Cultured KW - Arsenic Poisoning -- prevention & control KW - Humans KW - Adult KW - Oxidative Stress -- drug effects KW - Flavonoids -- pharmacology KW - Male KW - DNA Damage -- drug effects KW - Plant Extracts -- pharmacology KW - Arsenic -- toxicity KW - Antioxidants -- pharmacology KW - Tea -- chemistry KW - Lymphocytes -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70064155?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+environmental+pathology%2C+toxicology+and+oncology+%3A+official+organ+of+the+International+Society+for+Environmental+Toxicology+and+Cancer&rft.atitle=In+vitro+mitigation+of+arsenic+toxicity+by+tea+polyphenols+in+human+lymphocytes.&rft.au=Sinha%2C+Dona%3BDey%2C+Subhabrata%3BBhattacharya%2C+Rathindra+Kumar%3BRoy%2C+Madhumita&rft.aulast=Sinha&rft.aufirst=Dona&rft.date=2007-01-01&rft.volume=26&rft.issue=3&rft.spage=207&rft.isbn=&rft.btitle=&rft.title=Journal+of+environmental+pathology%2C+toxicology+and+oncology+%3A+official+organ+of+the+International+Society+for+Environmental+Toxicology+and+Cancer&rft.issn=07318898&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-01-25 N1 - Date created - 2008-01-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - BP1 transcriptionally activates bcl-2 and inhibits TNFalpha-induced cell death in MCF7 breast cancer cells. AN - 70054424; 17854498 AB - We have previously shown that the Beta Protein 1 (BP1) homeodomain protein is expressed in 81% of invasive ductal breast carcinomas, and that increased BP1 expression correlates with tumor progression. The purpose of our current investigation was to determine whether elevated levels of BP1 in breast cancer cells are associated with increased cell survival. Effects on cell viability and apoptosis of MCF7 cells stably overexpressing BP1 were determined using MTT and Annexin V assays, and through examination of caspase activation. TNFalpha was used to induce apoptosis. The potential regulation of apoptosis-associated genes by BP1 was studied using real-time PCR and western blot analyses. Electrophoretic mobility shift assays, site-directed mutagenesis, and transient assays were performed to specifically characterize the interaction of BP1 with the promoter of the bcl-2 gene. Stable overexpression of BP1 led to inhibition of apoptosis in MCF7 breast cancer cells challenged with TNFalpha. Increased BP1 resulted in reduced processing and activation of caspase-7, caspase-8, and caspase-9, and inactivation of the caspase substrate Poly(ADP-Ribose) Polymerase (PARP). Increased levels of full-length PARP and a decrease in procaspase-8 were also associated with BP1 overexpression. The bcl-2 gene is a direct target of BP1 since: (i) BP1 protein bound to a consensus binding sequence upstream of the bcl-2 P1 promoter in vitro. (ii) MCF7 cells overexpressing BP1 showed increased levels of bcl-2 mRNA and protein. (iii) Transient assays indicated that increased bcl-2 promoter activity is due to direct binding and modulation by BP1 protein. BP1 expression also prevented TNFalpha-mediated downregulation of bcl-2 mRNA and protein. These findings suggest mechanisms by which increased BP1 may impart a survival advantage to breast cancer cells, which could lead to increased resistance to therapeutic agents in patients. JF - Breast cancer research : BCR AU - Stevenson, Holly S AU - Fu, Sidney W AU - Pinzone, Joseph J AU - Rheey, Jinguen AU - Simmens, Samuel J AU - Berg, Patricia E AD - Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 1 VL - 9 IS - 5 KW - Annexin A5 KW - 0 KW - DLX4 protein, human KW - Homeodomain Proteins KW - Proto-Oncogene Proteins c-bcl-2 KW - Transcription Factors KW - Tumor Necrosis Factor-alpha KW - Luciferases KW - EC 1.13.12.- KW - Poly(ADP-ribose) Polymerases KW - EC 2.4.2.30 KW - Caspases KW - EC 3.4.22.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Promoter Regions, Genetic KW - Blotting, Western KW - Tumor Cells, Cultured KW - Humans KW - Genetic Vectors KW - Electrophoretic Mobility Shift Assay KW - Luciferases -- metabolism KW - Annexin A5 -- metabolism KW - Poly(ADP-ribose) Polymerases -- metabolism KW - Caspases -- metabolism KW - Gene Expression Regulation, Neoplastic KW - Breast Neoplasms -- genetics KW - Breast Neoplasms -- pathology KW - Transcription Factors -- metabolism KW - Tumor Necrosis Factor-alpha -- pharmacology KW - Proto-Oncogene Proteins c-bcl-2 -- metabolism KW - Apoptosis -- drug effects KW - Homeodomain Proteins -- metabolism KW - Breast Neoplasms -- metabolism KW - Transcription, Genetic KW - Proto-Oncogene Proteins c-bcl-2 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70054424?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Breast+cancer+research+%3A+BCR&rft.atitle=BP1+transcriptionally+activates+bcl-2+and+inhibits+TNFalpha-induced+cell+death+in+MCF7+breast+cancer+cells.&rft.au=Stevenson%2C+Holly+S%3BFu%2C+Sidney+W%3BPinzone%2C+Joseph+J%3BRheey%2C+Jinguen%3BSimmens%2C+Samuel+J%3BBerg%2C+Patricia+E&rft.aulast=Stevenson&rft.aufirst=Holly&rft.date=2007-01-01&rft.volume=9&rft.issue=5&rft.spage=R60&rft.isbn=&rft.btitle=&rft.title=Breast+cancer+research+%3A+BCR&rft.issn=1465-542X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-03-11 N1 - Date created - 2008-01-16 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Cell Biol. 1996 Oct;16(10):5546-56 [8816467] Curr Opin Pharmacol. 2006 Aug;6(4):364-8 [16753340] Cell. 1998 Aug 21;94(4):481-90 [9727491] Cell. 1998 Aug 21;94(4):491-501 [9727492] Cancer Res. 1998 Nov 1;58(21):4940-6 [9810003] Clin Cancer Res. 1996 Jul;2(7):1163-7 [9816283] J Cell Biol. 1999 Jan 25;144(2):281-92 [9922454] Blood Cells Mol Dis. 1998 Sep;24(3):356-69 [10087993] J Biol Chem. 1999 Nov 5;274(45):32099-107 [10542244] J Biol Chem. 2000 Mar 31;275(13):9303-7 [10734071] Nature. 2000 Jun 22;405(6789):974-8 [10879542] Leukemia. 2000 Nov;14(11):1867-75 [11069021] J Cell Physiol. 2001 Aug;188(2):161-9 [11424082] Cancer Res. 2001 Sep 1;61(17):6532-9 [11522651] J Allergy Clin Immunol. 2001 Oct;108(4 Suppl):S99-103 [11586274] Oncogene. 2001 Oct 25;20(48):7128-33 [11704839] Gene. 2001 Oct 31;278(1-2):131-9 [11707330] Trends Cell Biol. 2001 Dec;11(12):526-34 [11719060] J Steroid Biochem Mol Biol. 2001 Nov;78(5):409-18 [11738551] Cell. 2002 Jan 25;108(2):153-64 [11832206] Mol Cell Biol. 2002 Apr;22(8):2505-14 [11909945] Pharmacol Rev. 2002 Sep;54(3):375-429 [12223530] J Biol Chem. 2003 Feb 28;278(9):7580-90 [12482855] Breast Cancer Res. 2003;5(4):R82-7 [12817998] Oncogene. 2003 Oct 20;22(47):7414-30 [14576849] Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6478-83 [15123840] Cancer Biol Ther. 2004 Jun;3(6):568-72 [15044858] EMBO J. 1988 Jan;7(1):123-31 [2834197] J Pathol. 2005 Jan;205(2):154-71 [15643670] Oncogene. 2005 Feb 24;24(9):1648-52 [15674342] Breast Cancer Res Treat. 2005 Apr;90(3):241-7 [15830137] J Biol Chem. 1998 Apr 17;273(16):9357-60 [9545256] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Serum proteins and paraproteins in women with silicone implants and connective tissue disease: a case-control study. AN - 70049429; 17875216 AB - Prior studies have suggested abnormalities of serum proteins, including paraproteins, in women with silicone implants but did not control for the presence of connective-tissue disease (CTD). This retrospective case-control study, performed in tertiary-care academic centers, assessed possible alterations of serum proteins, including paraproteins, in such a population. Seventy-four women with silicone implants who subsequently developed CTD, and 74 age-matched and CTD-matched women without silicone implants, were assessed in the primary study; other groups were used for additional comparisons. Routine serum protein determinations and high-sensitivity protein electrophoresis and immunofixation electrophoresis were performed for detection of paraproteins. Women with silicone implants, either with or without CTD, had significantly lower serum total protein and alpha1-globulin, alpha2-globulin, beta-globulin, gamma-globulin, and IgG levels compared with those without silicone implants. There was no significant difference, however, in the frequency of paraproteinemia between women with silicone implants and CTD (9.5%) and age-matched and CTD-matched women without silicone implants (5.4%) (odds ratio, 1.82; 95% confidence interval, 0.51-6.45). Paraprotein isotypes were similar in the two groups, and the clinical characteristics of the 13 women with paraproteinemia were comparable with an independent population of 10 women with silicone breast implants, CTD, and previously diagnosed monoclonal gammopathies. In summary, this first comprehensive study of serum proteins in women with silicone implants and CTD found no substantially increased risk of monoclonal gammopathy. Women with silicone implants, however, had unexpectedly low serum globulin and immunoglobulin levels, with or without the subsequent development of CTD. The causes and clinical implications of these findings require further investigation. JF - Arthritis research & therapy AU - Csako, Gyorgy AU - Costello, Rene AU - Shamim, Ejaz A AU - O'Hanlon, Terrance P AU - Tran, Anthony AU - Clauw, Daniel J AU - Williams, H James AU - Miller, Frederick W AD - Department of Laboratory Medicine, Clinical Center, NIH, DHHS, 9000 Rockville Pike, Bethesda, MD 20892, USA. gcsako@nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1 VL - 9 IS - 5 KW - Blood Proteins KW - 0 KW - Paraproteins KW - Silicone Gels KW - Index Medicus KW - Paraproteinemias -- blood KW - Prospective Studies KW - Risk Factors KW - Humans KW - Paraproteinemias -- diagnosis KW - Adult KW - Case-Control Studies KW - Aged KW - Middle Aged KW - Paraproteinemias -- etiology KW - Female KW - Connective Tissue Diseases -- diagnosis KW - Connective Tissue Diseases -- etiology KW - Paraproteins -- metabolism KW - Silicone Gels -- adverse effects KW - Connective Tissue Diseases -- blood KW - Blood Proteins -- metabolism KW - Breast Implants -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/70049429?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Arthritis+research+%26+therapy&rft.atitle=Serum+proteins+and+paraproteins+in+women+with+silicone+implants+and+connective+tissue+disease%3A+a+case-control+study.&rft.au=Csako%2C+Gyorgy%3BCostello%2C+Rene%3BShamim%2C+Ejaz+A%3BO%27Hanlon%2C+Terrance+P%3BTran%2C+Anthony%3BClauw%2C+Daniel+J%3BWilliams%2C+H+James%3BMiller%2C+Frederick+W&rft.aulast=Csako&rft.aufirst=Gyorgy&rft.date=2007-01-01&rft.volume=9&rft.issue=5&rft.spage=R95&rft.isbn=&rft.btitle=&rft.title=Arthritis+research+%26+therapy&rft.issn=1478-6362&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-04-08 N1 - Date created - 2008-01-16 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: N Engl J Med. 2000 Mar 16;342(11):781-90 [10717013] J Natl Cancer Inst. 1994 Jul 20;86(14):1058-65 [8021954] Aust N Z J Med. 1999 Aug;29(4):500-4 [10868527] Leukemia. 2000 Nov;14(11):1975-9 [11069034] Arch Intern Med. 2001 Mar 26;161(6):864-7 [11268230] Ann Plast Surg. 2004 Nov;53(5):413-9 [15502454] Acta Med Scand. 1966 Feb;179(2):235-47 [4160039] Ann N Y Acad Sci. 1971 Dec 31;190:507-18 [5003017] Medicine (Baltimore). 1977 Jul;56(4):255-86 [327194] J Clin Pathol. 1982 Jan;35(1):63-8 [6801095] Scand J Haematol. 1986 Jan;36(1):103-6 [3081993] J Rheumatol. 1988 Jun;15(6):894-8 [3262160] Arthritis Rheum. 1990 Feb;33(2):160-72 [2306288] Recenti Prog Med. 1990 May;81(5):306-9 [2377807] Sangre (Barc). 1991 Oct;36(5):377-82 [1816635] Autoimmunity. 1992;13(2):101-5 [1467431] Ann Intern Med. 1993 Jun 15;118(12):929-36 [8489106] Br J Surg. 1993 Sep;80(9):1097-100 [8402103] FASEB J. 1993 Oct;7(13):1265-8 [8405812] J Natl Cancer Inst. 1994 Sep 21;86(18):1424 [8072038] Keio J Med. 1994 Jun;43(2):79-87 [8089958] JAMA. 1995 Jan 11;273(2):116 [7799490] Curr Top Microbiol Immunol. 1996;210:337-53 [8565576] Curr Top Microbiol Immunol. 1996;210:357-9 [8565577] Curr Top Microbiol Immunol. 1996;210:361-6 [8565578] Curr Top Microbiol Immunol. 1996;210:367-74 [8565579] Curr Top Microbiol Immunol. 1996;210:375-83 [8565580] Curr Top Microbiol Immunol. 1996;210:397-407 [8565584] Curr Top Microbiol Immunol. 1996;210:411-7 [8565585] Leukemia. 1996 Feb;10(2):327-32 [8637242] Regul Toxicol Pharmacol. 1996 Feb;23(1 Pt 1):74-85 [8628923] Blood. 1996 Jun 1;87(11):4762-9 [8639847] Ann Plast Surg. 1995 Dec;35(6):561-70 [8748335] N Engl J Med. 1997 Mar 6;336(10):677-82 [9041097] Arthritis Rheum. 1997 Sep;40(9):1725 [9324032] Blood. 1997 Nov 1;90(9):3682-90 [9345053] Pathobiology. 1998;66(6):302-5 [9769477] J Rheumatol. 1999 Jan;26(1):73-7 [9918243] Pathol Biol (Paris). 1999 Feb;47(2):119-27 [10192879] J Rheumatol. 1999 Apr;26(4):816-25 [10229402] Am J Med. 1999 Jan;106(1):11-9 [10320112] J Biomed Mater Res. 1999 Jul;46(1):132-4 [10357144] Biomaterials. 1999 Jun;20(11):1063-9 [10378807] Postgrad Med. 1999 Aug;106(2):135-42; quiz 185 [10456045] Arthritis Rheum. 2004 Nov;50(11):3646-50 [15529361] Clin Lymphoma Myeloma. 2005 Sep;6(2):102-14 [16231848] N Engl J Med. 2006 Mar 30;354(13):1362-9 [16571879] Clin Diagn Lab Immunol. 2000 May;7(3):366-70 [10799447] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Appetitive conditioning: neural bases and implications for psychopathology. AN - 69024396; 17210179 AB - Appetitive conditioning is the process through which new rewards are learned and acquire their motivational salience. Although it has the same evolutionary survival significance as aversive conditioning, appetitive conditioning has rarely been studied in humans. This gap may be explained by the difficulty to find in humans suitable appetitive stimuli that can elicit physiological responses similar to those elicited by aversive stimuli. To help remedy this gap, we review the literature on conditioning, with emphasis on appetitive conditioning. This review comprises three parts. First, we examine the different forms of conditioning. Second, we review the neural basis of appetitive conditioning, particularly from a functional neuroimaging perspective. And third, we demonstrate how perturbations in processes involved in appetitive conditioning can contribute to implicated psychopathologies and suggest neurobiological models underlying these pathologies. The ultimate goal of this review is to stimulate new avenues of research that have direct links to molecular biology, and thus could prove to be invaluable to progress in the understanding and treatment of psychiatric disabilities. JF - Neuroscience and biobehavioral reviews AU - Martin-Soelch, C AU - Linthicum, J AU - Ernst, M AD - Mood and Anxiety Disorders Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 426 EP - 440 VL - 31 IS - 3 SN - 0149-7634, 0149-7634 KW - Index Medicus KW - Substance-Related Disorders -- physiopathology KW - Depression -- physiopathology KW - Animals KW - Feeding and Eating Disorders -- physiopathology KW - Conditioning, Operant -- physiology KW - Humans KW - Association Learning -- physiology KW - Schizophrenia -- physiopathology KW - Reward KW - Conditioning, Classical -- physiology KW - Mental Disorders -- psychology KW - Brain -- physiology KW - Appetitive Behavior -- physiology KW - Mental Disorders -- physiopathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/69024396?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+and+biobehavioral+reviews&rft.atitle=Appetitive+conditioning%3A+neural+bases+and+implications+for+psychopathology.&rft.au=Martin-Soelch%2C+C%3BLinthicum%2C+J%3BErnst%2C+M&rft.aulast=Martin-Soelch&rft.aufirst=C&rft.date=2007-01-01&rft.volume=31&rft.issue=3&rft.spage=426&rft.isbn=&rft.btitle=&rft.title=Neuroscience+and+biobehavioral+reviews&rft.issn=01497634&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-28 N1 - Date created - 2007-02-19 N1 - Date revised - 2017-01-14 N1 - SuppNotes - Cited By: Psychopharmacology (Berl). 1984;84(3):405-12 [6440188] Psychopharmacology (Berl). 1986;90(3):390-7 [3097729] Behav Res Ther. 1989;27(3):279-87 [2730509] Psychol Rev. 1990 Apr;97(2):147-68 [2186423] Addict Behav. 1990;15(4):387-93 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[13449253] Trends Cogn Sci. 2004 Dec;8(12):539-46 [15556023] J Neurosci. 2005 Mar 9;25(10):2733-40 [15758183] Neuron. 2005 Apr 21;46(2):321-31 [15848809] Curr Opin Neurol. 2005 Aug;18(4):411-7 [16003117] Am J Psychiatry. 2005 Aug;162(8):1403-13 [16055761] Nat Neurosci. 2005 Sep;8(9):1234-40 [16116445] Neuron. 2005 Sep 1;47(5):633-6 [16129393] J Anat. 2005 Sep;207(3):271-82 [16185252] Nicotine Tob Res. 2004 Jun;6(3):523-32 [15203786] Neuropharmacology. 2004;47 Suppl 1:3-13 [15464121] Behav Res Ther. 1975 Oct;13(4):221-6 [1191163] Science. 1983 Apr 22;220(4595):431-3 [6836286] Proc Natl Acad Sci U S A. 1984 Aug;81(15):4998-5001 [6589643] N1 - Last updated - 2017-01-19 ER - TY - JOUR T1 - A favorable view: progress in cancer prevention and screening. AN - 69009121; 17302181 AB - Clifton Leaf, in his article "Why We're Losing the War on Cancer," presents criticisms of past research approaches and the small impact of this research thus far on producing cures or substantially extending the life of many cancer patients. It is true that gains in long-term survival for people with advanced cancers have been modest, hindered in part by the heterogeneity of tumors, which allows the cancers to persist using alternate molecular pathways and so evade many cancer therapeutics. In contrast, clinical trials have demonstrated that it is possible to reduce the incidence or improve cancer survival through prevention and early detection. Strides have been made in preventing or detecting early the four deadliest cancers in the United States (i.e., lung, breast, prostate, and colorectal). For example, 7-year follow-up data from the Breast Cancer Prevention Trial (BCPT) provides evidence that tamoxifen reduces the occurrence of invasive breast tumors by more than 40%; recent studies using aromatase inhibitors and raloxifene are also promising. The Prostate Cancer Prevention Trial (PCPT) showed that finasteride reduced prostate cancer incidence by 25%, and the ongoing Selenium and Vitamin E Cancer Prevention Trial (SELECT) is investigating selenium and vitamin E for prostate cancer prevention based on encouraging results from earlier studies. Living a healthy lifestyle, including regular physical activity, avoiding obesity, and eating primarily a plant-based diet has been associated with a lower risk of colorectal cancer. In addition, noninvasive stool DNA tests for early detection are being studied, which may lessen the reluctance of people to be screened for colorectal polyps and cancer. Behavioral and medical approaches for smoking prevention are ways to reduce the incidence of lung cancer, with antinicotine vaccines on the horizon that may help former smokers to avoid relapse. The US National Lung Screening Trial is testing whether early detection via spiral CT screening will reduce lung cancer mortality. Prevention and earlier detection offer efficient and practical strategies to reduce the cancer burden. Several of the suggestions Mr. Leaf makes, such as developing interdisciplinary collaborations and allocating resources to research earlier in the process of carcinogenesis, have become an integral strategy in the National Cancer Institute's (NCI) approach in the past decade, specifically in the realm of cancer prevention and early detection. For example, an aggressive program to identify biomarkers for earlier detection of cancer--the NCI's Early Detection Research Detection (EDRN)--has identified three promising biomarkers since its establishment in 2000. It collaborates with the National Institute of Standards and Technology and extramural scientists to develop validation standards and to identify the best technologies to use for systematic investigations. If these biomarkers can be validated, they might help to reduce cancer mortality. JF - Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer AU - Greenwald, Peter AD - Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-7309, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 3 EP - 17 VL - 174 SN - 0080-0015, 0080-0015 KW - Index Medicus KW - Humans KW - Clinical Trials as Topic KW - Mass Screening -- methods KW - Neoplasms -- prevention & control KW - Early Diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/69009121?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Recent+results+in+cancer+research.+Fortschritte+der+Krebsforschung.+Progres+dans+les+recherches+sur+le+cancer&rft.atitle=A+favorable+view%3A+progress+in+cancer+prevention+and+screening.&rft.au=Greenwald%2C+Peter&rft.aulast=Greenwald&rft.aufirst=Peter&rft.date=2007-01-01&rft.volume=174&rft.issue=&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Recent+results+in+cancer+research.+Fortschritte+der+Krebsforschung.+Progres+dans+les+recherches+sur+le+cancer&rft.issn=00800015&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-16 N1 - Date created - 2007-02-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Neurological aspects of chemical terrorism. AN - 68970802; 17262854 JF - Annals of neurology AU - Jett, David A AD - National Institutes of Health, National Institute of Neurological Disorders and Stroke, Office of Technology Development, Bethesda, MD 20892-9527, USA. jettd@ninds.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 9 EP - 13 VL - 61 IS - 1 SN - 0364-5134, 0364-5134 KW - Chemical Warfare Agents KW - 0 KW - Index Medicus KW - Humans KW - Chemical Warfare Agents -- poisoning KW - Nervous System Diseases -- etiology KW - Chemical Terrorism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68970802?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annals+of+neurology&rft.atitle=Neurological+aspects+of+chemical+terrorism.&rft.au=Jett%2C+David+A&rft.aulast=Jett&rft.aufirst=David&rft.date=2007-01-01&rft.volume=61&rft.issue=1&rft.spage=9&rft.isbn=&rft.btitle=&rft.title=Annals+of+neurology&rft.issn=03645134&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-27 N1 - Date created - 2007-02-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Exploiting structurally diverse nucleoside analogs as probes of reverse transcription complexes. AN - 68968960; 17266554 AB - Crystal structures of HIV-1 reverse transcriptase (RT) in complex with either duplex DNA or an RNA/DNA hybrid have provided significant insights into the manner in which this highly versatile enzyme accommodates the conformationally-distinct nucleic acid substrates encountered during the reverse transcription cycle. Biochemical data likewise suggest that unique structural features of the nucleic acid substrates contribute towards recognition by their cognate RT. While site-directed mutagenesis of catalytically- and structurally-critical protein motifs is relatively facile, understanding how nucleic acid structure contributes to its recognition presents a greater challenge. The relative ease with which large DNA and RNA fragments can now be chemically synthesized, in conjunction with the increased availability of ribo- and deoxyribonucleoside analogs, allows nucleic acid structure to be examined with respect to the role of hydrogen bonding, nucleobase stacking, sugar ring geometry and charge of the phosphodiester backbone. This review summarizes our use of nucleoside analogs to understand how the structure of cis-acting regulatory signals mediates their recognition by structurally diverse retroviral and retrotransposon enzymes. JF - Current HIV research AU - Rausch, Jason W AU - Le Grice, Stuart F J AD - RT Biochemistry Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick MD 21702, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 11 EP - 22 VL - 5 IS - 1 KW - DNA, Viral KW - 0 KW - Nucleosides KW - RNA, Viral KW - HIV Reverse Transcriptase KW - EC 2.7.7.49 KW - Index Medicus KW - HIV-1 -- genetics KW - DNA, Viral -- biosynthesis KW - DNA, Viral -- chemistry KW - RNA, Viral -- chemistry KW - Hydrogen Bonding KW - HIV Reverse Transcriptase -- chemistry KW - Reverse Transcription KW - Nucleosides -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68968960?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+HIV+research&rft.atitle=Exploiting+structurally+diverse+nucleoside+analogs+as+probes+of+reverse+transcription+complexes.&rft.au=Rausch%2C+Jason+W%3BLe+Grice%2C+Stuart+F+J&rft.aulast=Rausch&rft.aufirst=Jason&rft.date=2007-01-01&rft.volume=5&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Current+HIV+research&rft.issn=1873-4251&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-20 N1 - Date created - 2007-02-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - miRNAs in the biology of cancers and viral infections. AN - 68966030; 17266578 AB - MicroRNAs (miRNAs) are non-coding small RNAs that play important roles in a variety of biological pathways including cellular proliferation and apoptosis. Recent studies have linked the expression of selected miRNAs to carcinogenesis and viral pathogenesis. Here, we will discuss examples of roles served by cellular miRNAs and virus-encoded miRNAs in the development of cancers and viral diseases. JF - Current medicinal chemistry AU - Yeung, Man Lung AU - Bennasser, Yamina AU - Jeang, Kuan-Teh AD - Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0460, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 191 EP - 197 VL - 14 IS - 2 SN - 0929-8673, 0929-8673 KW - MicroRNAs KW - 0 KW - Index Medicus KW - Apoptosis KW - Humans KW - Cell Proliferation KW - Virus Diseases -- genetics KW - MicroRNAs -- physiology KW - Neoplasms -- genetics KW - Neoplasms -- etiology KW - Virus Diseases -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68966030?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+medicinal+chemistry&rft.atitle=miRNAs+in+the+biology+of+cancers+and+viral+infections.&rft.au=Yeung%2C+Man+Lung%3BBennasser%2C+Yamina%3BJeang%2C+Kuan-Teh&rft.aulast=Yeung&rft.aufirst=Man&rft.date=2007-01-01&rft.volume=14&rft.issue=2&rft.spage=191&rft.isbn=&rft.btitle=&rft.title=Current+medicinal+chemistry&rft.issn=09298673&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-27 N1 - Date created - 2007-02-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Bleeding patterns after misoprostol vs surgical treatment of early pregnancy failure: results from a randomized trial. AN - 68934541; 17240222 AB - The purpose of this study was to describe bleeding patterns after misoprostol or curettage for early pregnancy failure (EPF). This was a randomized trial that included women (n = 652) with EPF. Participants were assigned to vaginal misoprostol (800 microg) or curettage in a 3:1 ratio. Participants completed a bleeding diary. We measured hemoglobin levels at baseline and 2 weeks after the treatment. Decreases in hemoglobin levels were greater after misoprostol (-0.7 g/dL; SD, 1.2) than curettage (-0.2 g/dL; SD, 0.9; P < .001). Large changes in hemoglobin levels (at least 2 g/dL) or low nadir hemoglobin levels (< 10 g/dL) were more frequent after misoprostol (55/428 women; 12.8%) than after curettage (6/135 women; 4.4%; P = .02). More participants in the misoprostol group reported "any bleeding" or "heavy bleeding" every study day. Four women who were treated with misoprostol required blood transfusion. Bleeding is heavier and more prolonged after medical treatment with misoprostol than with curettage for EPF; however, bleeding rarely requires intervention. JF - American journal of obstetrics and gynecology AU - Davis, Anne R AU - Hendlish, Sarah K AU - Westhoff, Carolyn AU - Frederick, Margaret M AU - Zhang, Jun AU - Gilles, Jerry M AU - Barnhart, Kurt AU - Creinin, Mitchell D AU - National Institute of Child Health and Human Development Management of Early Pregnancy Failure Trial AD - Columbia University, Department of Obstetrics & Gynecology, 622 West 168th St, PH 16 Room 80, New York, NY 10032, USA. ard4@columbia.edu ; National Institute of Child Health and Human Development Management of Early Pregnancy Failure Trial Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 31.e1 EP - 7 VL - 196 IS - 1 KW - Abortifacient Agents, Nonsteroidal KW - 0 KW - Misoprostol KW - 0E43V0BB57 KW - Abridged Index Medicus KW - Index Medicus KW - Humans KW - Adult KW - Female KW - Pregnancy KW - Misoprostol -- adverse effects KW - Curettage KW - Abortifacient Agents, Nonsteroidal -- adverse effects KW - Uterine Hemorrhage -- epidemiology KW - Abortion, Spontaneous -- surgery KW - Abortion, Spontaneous -- drug therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68934541?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+obstetrics+and+gynecology&rft.atitle=Bleeding+patterns+after+misoprostol+vs+surgical+treatment+of+early+pregnancy+failure%3A+results+from+a+randomized+trial.&rft.au=Davis%2C+Anne+R%3BHendlish%2C+Sarah+K%3BWesthoff%2C+Carolyn%3BFrederick%2C+Margaret+M%3BZhang%2C+Jun%3BGilles%2C+Jerry+M%3BBarnhart%2C+Kurt%3BCreinin%2C+Mitchell+D%3BNational+Institute+of+Child+Health+and+Human+Development+Management+of+Early+Pregnancy+Failure+Trial&rft.aulast=Davis&rft.aufirst=Anne&rft.date=2007-01-01&rft.volume=196&rft.issue=1&rft.spage=31.e1&rft.isbn=&rft.btitle=&rft.title=American+journal+of+obstetrics+and+gynecology&rft.issn=1097-6868&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-08 N1 - Date created - 2007-01-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Nuclear factor-kappaB p65 small interfering RNA or proteasome inhibitor bortezomib sensitizes head and neck squamous cell carcinomas to classic histone deacetylase inhibitors and novel histone deacetylase inhibitor PXD101. AN - 68933158; 17237265 AB - Histone deacetylase inhibitors (HDI) can inhibit proliferation and enhance apoptosis in a wide range of malignancies. However, HDIs show relatively modest activity in head and neck squamous cell carcinomas (HNSCC), in which we have shown the activation of nuclear factor-kappaB (NF-kappaB; NF-kappaB1/RelA or p50/p65), a transcription factor that promotes expression of proliferative and antiapoptotic genes. In this study, we examined if HDIs enhance activation of NF-kappaB and target genes and if genetic or pharmacologic inhibition of NF-kappaB can sensitize HNSCC to HDIs. Limited activity of classic HDIs trichostatin A and sodium butyrate was associated with enhanced activation of NF-kappaB reporter activity in a panel of six HNSCC cell lines. HDIs enhanced NF-kappaB p50/p65 DNA binding and acetylation of the RelA p65 subunit. Transfection of small interfering RNAs targeting p65 strongly inhibited NF-kappaB expression and activation, induced cell cycle arrest and cell death, and further sensitized HNSCC cells when combined with HDIs. The p65 small interfering RNA inhibited HDI-enhanced expression of several NF-kappaB-inducible genes implicated in oncogenesis of HNSCC, such as p21, cyclin D1, and BCL-XL. Bortezomib, an inhibitor of proteasome-dependent NF-kappaB activation, also increased sensitization to trichostatin A, sodium butyrate, and a novel HDI, PXD101, in vitro, and to the antitumor effects of PXD101 in bortezomib-resistant UMSCC-11A xenografts. However, gastrointestinal toxicity, weight loss, and mortality of the combination were dose limiting and required parenteral fluid administration. We conclude that HDI-enhanced NF-kappaB activation is one of the major mechanisms of resistance of HNSCC to HDIs. The combination of HDI and proteasome inhibitor produced increased antitumor activity. Low starting dosages for clinical studies combining HDIs with proteasome inhibitors and IV fluid support may be warranted. JF - Molecular cancer therapeutics AU - Duan, Jianming AU - Friedman, Jay AU - Nottingham, Liesl AU - Chen, Zhong AU - Ara, Gulshan AU - Van Waes, Carter AD - National Institute on Deafness and Other Communication Disorders, NIH, CRC Building 10, Room 4-2732, 10 Center Drive, Bethesda, MD 20892, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 37 EP - 50 VL - 6 IS - 1 SN - 1535-7163, 1535-7163 KW - Antineoplastic Agents KW - 0 KW - Boronic Acids KW - Cyclin-Dependent Kinase Inhibitor p21 KW - DNA, Neoplasm KW - Enzyme Inhibitors KW - Histone Deacetylase Inhibitors KW - Hydroxamic Acids KW - Inhibitor of Apoptosis Proteins KW - NF-kappa B p50 Subunit KW - Proteasome Inhibitors KW - Pyrazines KW - RNA, Small Interfering KW - Sulfonamides KW - Transcription Factor RelA KW - bcl-X Protein KW - Cyclin D1 KW - 136601-57-5 KW - Bortezomib KW - 69G8BD63PP KW - belinostat KW - F4H96P17NZ KW - Index Medicus KW - Animals KW - Acetylation -- drug effects KW - Antineoplastic Agents -- administration & dosage KW - Humans KW - Cyclin-Dependent Kinase Inhibitor p21 -- genetics KW - Mice KW - Cyclin D1 -- genetics KW - Drug Resistance, Neoplasm KW - bcl-X Protein -- metabolism KW - Protein Binding -- drug effects KW - Inhibitor of Apoptosis Proteins -- genetics KW - Antineoplastic Combined Chemotherapy Protocols KW - Xenograft Model Antitumor Assays KW - Enzyme Inhibitors -- pharmacology KW - Mice, SCID KW - Down-Regulation -- drug effects KW - Antineoplastic Agents -- pharmacology KW - DNA, Neoplasm -- metabolism KW - NF-kappa B p50 Subunit -- metabolism KW - Boronic Acids -- pharmacology KW - Hydroxamic Acids -- therapeutic use KW - Transcription Factor RelA -- metabolism KW - Pyrazines -- therapeutic use KW - Pyrazines -- pharmacology KW - Head and Neck Neoplasms -- pathology KW - RNA, Small Interfering -- metabolism KW - Head and Neck Neoplasms -- drug therapy KW - Pyrazines -- administration & dosage KW - Head and Neck Neoplasms -- enzymology KW - Hydroxamic Acids -- administration & dosage KW - Boronic Acids -- administration & dosage KW - Carcinoma, Squamous Cell -- pathology KW - Boronic Acids -- therapeutic use KW - Carcinoma, Squamous Cell -- drug therapy KW - Hydroxamic Acids -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68933158?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+cancer+therapeutics&rft.atitle=Nuclear+factor-kappaB+p65+small+interfering+RNA+or+proteasome+inhibitor+bortezomib+sensitizes+head+and+neck+squamous+cell+carcinomas+to+classic+histone+deacetylase+inhibitors+and+novel+histone+deacetylase+inhibitor+PXD101.&rft.au=Duan%2C+Jianming%3BFriedman%2C+Jay%3BNottingham%2C+Liesl%3BChen%2C+Zhong%3BAra%2C+Gulshan%3BVan+Waes%2C+Carter&rft.aulast=Duan&rft.aufirst=Jianming&rft.date=2007-01-01&rft.volume=6&rft.issue=1&rft.spage=37&rft.isbn=&rft.btitle=&rft.title=Molecular+cancer+therapeutics&rft.issn=15357163&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-16 N1 - Date created - 2007-01-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Fetal alcohol syndrome: historical perspectives. AN - 68920387; 17224346 AB - Fetal alcohol syndrome (FAS), the most severe manifestation of the adverse effects of alcohol on foetal development, was first described in the French medical literature by Lemoine et al. in 1968 [Les Gfants des parents alcholiques: anomalies observes a propos de 127 cas (The children of alchoholic parents: anomalies observed in 127 cases). Quert in Medicine 8, 476-482]. Five years later, Jones et al., 1973. Pattern of malformation in offspring of chronic alcholic mothers. Lancet 1, 1129-1267] were the first to delineate systematically the association between maternal alcohol abuse and a specific pattern of birth defects and to provide diagnostic criteria for this condition. Several diagnostic systems have since been developed with a view to capturing the wide spectrum of physical and behavioral anomalies resulting from prenatal alcohol exposure. The purpose of the current paper is to outline the evolution of FAS as a medical diagnosis. JF - Neuroscience and biobehavioral reviews AU - Calhoun, Faye AU - Warren, Kenneth AD - National Institute on Alcohol Abuse and Alcoholism, National Institute of Health, Bethesda, MD, USA. fcalhoun@willco.niaaa.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 168 EP - 171 VL - 31 IS - 2 SN - 0149-7634, 0149-7634 KW - Index Medicus KW - History, 21st Century KW - History, 20th Century KW - Humans KW - History, 18th Century KW - History, 19th Century KW - Female KW - Pregnancy KW - Fetal Alcohol Spectrum Disorders -- history KW - Fetal Alcohol Spectrum Disorders -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68920387?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroscience+and+biobehavioral+reviews&rft.atitle=Fetal+alcohol+syndrome%3A+historical+perspectives.&rft.au=Calhoun%2C+Faye%3BWarren%2C+Kenneth&rft.aulast=Calhoun&rft.aufirst=Faye&rft.date=2007-01-01&rft.volume=31&rft.issue=2&rft.spage=168&rft.isbn=&rft.btitle=&rft.title=Neuroscience+and+biobehavioral+reviews&rft.issn=01497634&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-17 N1 - Date created - 2007-01-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - CONF T1 - Pain, opioids, and addiction: an urgent problem for doctors and patients. AN - 68546640; 18032317 AB - In March 2007, the National Institute of Drug Abuse (NIDA) of the National Institutes of Health (NIH) conducted a meeting on opioid prescribing, chronic pain and prescription drug abuse in collaboration with the American Medical Association. The meeting was held on the NIH campus in Bethesda, Maryland. This report summarizes major presentations presented at the meeting. JF - Journal of pain & palliative care pharmacotherapy AU - National Institute of Drug Abuse AU - National Institutes of Health Y1 - 2007 PY - 2007 DA - 2007 SP - 45 EP - 49 VL - 21 IS - 4 KW - Analgesics, Opioid KW - 0 KW - Dosage Forms KW - Receptors, Opioid KW - Index Medicus KW - Smoking KW - Humans KW - Palliative Care KW - Pain Measurement KW - Chronic Disease KW - Behavior, Addictive -- genetics KW - Receptors, Opioid -- physiology KW - Practice Patterns, Physicians' KW - Pain -- drug therapy KW - Opioid-Related Disorders -- epidemiology KW - Analgesics, Opioid -- therapeutic use KW - Pain -- genetics KW - Analgesics, Opioid -- adverse effects KW - Opioid-Related Disorders -- etiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68546640?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Journal+of+pain+%26+palliative+care+pharmacotherapy&rft.atitle=Pain%2C+opioids%2C+and+addiction%3A+an+urgent+problem+for+doctors+and+patients.&rft.au=National+Institute+of+Drug+Abuse%3BNational+Institutes+of+Health&rft.aulast=National+Institute+of+Drug+Abuse&rft.aufirst=&rft.date=2007-01-01&rft.volume=21&rft.issue=4&rft.spage=45&rft.isbn=&rft.btitle=&rft.title=Journal+of+pain+%26+palliative+care+pharmacotherapy&rft.issn=15360288&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-01-29 N1 - Date created - 2007-11-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Carcinogenicity of agricultural pesticides in adults and children. AN - 68543888; 18032335 AB - The role of specific agricultural pesticides in relation to adult and childhood cancers has not been firmly established due to the lack of precise exposure data in previous studies. Improvements in exposure assessment, disease classification, and application of molecular techniques in recent epidemiological evaluations is rapidly improving our ability to evaluate the human carcinogenicity of agricultural pesticides. The role of pesticide exposures in the etiology of human cancer is outlined by anatomical site and recent development in exposure assessment and molecular epidemiology are summarized and evaluated. JF - Journal of agromedicine AU - Alavanja, Michael C R AU - Ward, Mary H AU - Reynolds, Peggy AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD 20892, USA. alavanjm@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 39 EP - 56 VL - 12 IS - 1 SN - 1059-924X, 1059-924X KW - Pesticides KW - 0 KW - Index Medicus KW - Occupational Exposure KW - Humans KW - Adult KW - Environmental Exposure KW - Child KW - Neoplasms -- chemically induced KW - Agricultural Workers' Diseases -- chemically induced KW - Pesticides -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68543888?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+agromedicine&rft.atitle=Carcinogenicity+of+agricultural+pesticides+in+adults+and+children.&rft.au=Alavanja%2C+Michael+C+R%3BWard%2C+Mary+H%3BReynolds%2C+Peggy&rft.aulast=Alavanja&rft.aufirst=Michael+C&rft.date=2007-01-01&rft.volume=12&rft.issue=1&rft.spage=39&rft.isbn=&rft.btitle=&rft.title=Journal+of+agromedicine&rft.issn=1059924X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-01-03 N1 - Date created - 2007-11-22 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The yin and yang of stem cell gene therapy: insights into hematopoiesis, leukemogenesis, and gene therapy safety. AN - 68527386; 18024665 AB - Over the past decade, success in the treatment of serious genetic disorders via gene therapy was finally achieved. However, this progress was tempered by the occurrence of serious adverse events related to vector integration into the genome and activation of adjacent proto-oncogenes. Investigators are now focused on retaining the clinical potential of integrating vectors while decreasing the risk of insertional mutagenesis. JF - Hematology. American Society of Hematology. Education Program AU - Dunbar, Cynthia E AD - Hematology Branch, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA. dunbarc@nhlbi.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 460 EP - 465 SN - 1520-4391, 1520-4391 KW - Index Medicus KW - Humans KW - Genetic Vectors KW - Virus Integration KW - Retroviridae -- genetics KW - Genetic Therapy -- adverse effects KW - Leukemia -- etiology KW - Hematopoiesis -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68527386?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Hematology.+American+Society+of+Hematology.+Education+Program&rft.atitle=The+yin+and+yang+of+stem+cell+gene+therapy%3A+insights+into+hematopoiesis%2C+leukemogenesis%2C+and+gene+therapy+safety.&rft.au=Dunbar%2C+Cynthia+E&rft.aulast=Dunbar&rft.aufirst=Cynthia&rft.date=2007-01-01&rft.volume=&rft.issue=&rft.spage=460&rft.isbn=&rft.btitle=&rft.title=Hematology.+American+Society+of+Hematology.+Education+Program&rft.issn=15204391&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2009-07-14 N1 - Date created - 2007-11-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Familial CLL: genes and environment. AN - 68527340; 18024649 AB - Families with multiple individuals affected with chronic lymphocytic leukemia (CLL) and other related B-cell tumors have been described in the literature. Familial CLL does not appear to differ from sporadic CLL in terms of prognostic markers and clinical outcome. While some environmental factors (such as farming-related exposures and occupational chemicals) may increase risk of CLL, results of epidemiologic studies have been generally inconsistent. Rates of CLL in the population show significant international variation, with the highest rates in the U.S. and Europe and the lowest rates in Asia. Migrants from Asia to the U.S. also have low rates of CLL, which supports a greater role for genetic compared with environmental risk factors. Large, population-based case-control and cohort studies have also shown significant familial aggregation of CLL and related conditions including non-Hodgkin and Hodgkin lymphoma. Monoclonal B-cell lymphocytosis also aggregates in families with CLL. However, the clinical implication of familial aggregation is minimal given the overall rarity of CLL. Linkage studies have been conducted in high-risk CLL families to screen the whole genome for loci that contribute to susceptibility, but no gene mutations have yet been identified by this method. Association studies of candidate genes have implicated immune function and other genes, but more studies are needed to verify these findings. The ability to conduct large-scale genomic studies will play an important role in detecting susceptibility genes for CLL over the next few years and thereby help to delineate etiologic pathways. JF - Hematology. American Society of Hematology. Education Program AU - Goldin, Lynn R AU - Slager, Susan L AD - Genetic Epidemiology Branch, DCEG, NCI, 6120 Executive Blvd. Rm 7008, MSC 7236, Bethesda, MD 20892-7236, USA. goldinl@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 339 EP - 345 SN - 1520-4391, 1520-4391 KW - Index Medicus KW - Risk Factors KW - Humans KW - Genetic Predisposition to Disease KW - Leukemia, Lymphocytic, Chronic, B-Cell -- genetics KW - Environmental Exposure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68527340?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Hematology.+American+Society+of+Hematology.+Education+Program&rft.atitle=Familial+CLL%3A+genes+and+environment.&rft.au=Goldin%2C+Lynn+R%3BSlager%2C+Susan+L&rft.aulast=Goldin&rft.aufirst=Lynn&rft.date=2007-01-01&rft.volume=&rft.issue=&rft.spage=339&rft.isbn=&rft.btitle=&rft.title=Hematology.+American+Society+of+Hematology.+Education+Program&rft.issn=15204391&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2009-07-14 N1 - Date created - 2007-11-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Viral determinants of human papillomavirus persistence following loop electrical excision procedure treatment for cervical intraepithelial neoplasia grade 2 or 3. AN - 68422301; 17220326 AB - Persistent infection with carcinogenic human papillomavirus (HPV) causes cervical precancer (cervical intraepithelial neoplasia grade 2+) which, in the United States, is commonly treated using the loop electrical excision procedure (LEEP). Data from Atypical Squamous Cells of Undetermined Significance-Low-Grade Squamous Intraepithelial Lesion Triage Study were used to evaluate HPV persistence and reappearance after LEEP. Cervical specimens, collected before LEEP and at 6-month study visits, were tested by L1-PCR for detection of >or=27 HPV types. HPV persistence, defined as the same HPV type being present before and 6 months after LEEP, was evaluated by: (a) genotype, (b) carcinogenicity, and (c) phylogenetic species. HPV infections that cleared post-LEEP (the complement of persistence) were followed for reappearance of the same type. HPV infections (n = 1,130) were detected among 481 women who underwent LEEP. Overall, 20.4% [95% confidence interval (95% CI), 18.2-22.9%] of infections persisted. Assessment of heterogeneity within the three categorizations of HPV showed that phylogenetic species best fit the data. Persistence was significantly greater by HPV types in the alpha3 species [all are noncarcinogenic; 40.9% (95% CI, 31.8-50.4%)] compared with HPV types in the alpha9 (HPV16 and related types) and alpha7 species (HPV18 and related types; 17.6% and 17.9%, respectively; P < 0.001 for both). HPV reappeared in 7.8% (95% CI, 6.1-9.9%) of infections that cleared after LEEP. Infections in the alpha3 species (22.6%) were the most likely to reappear compared with HPV types in the alpha9 (7.5%) and alpha7 (6.8%) species. Patterns of HPV persistence and reappearance following LEEP were better explained by phylogenetic rather than standard classifications. HPV types likely to persist after LEEP as well as those likely to repopulate the cervical/vaginal epithelium were those in the alpha3 species, which are in effect not treated, but are not associated with cervical cancer and are unlikely to cause disease. JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology AU - Kreimer, Aimée R AU - Katki, Hormuzd A AU - Schiffman, Mark AU - Wheeler, Cosette M AU - Castle, Philip E AU - ASCUS-LSIL Triage Study Group AD - Division of Cancer Prevention, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. kreimera@mail.nih.gov ; ASCUS-LSIL Triage Study Group Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 11 EP - 16 VL - 16 IS - 1 SN - 1055-9965, 1055-9965 KW - Index Medicus KW - Randomized Controlled Trials as Topic KW - Risk Factors KW - Humans KW - Predictive Value of Tests KW - Follow-Up Studies KW - United States -- epidemiology KW - Female KW - Papillomavirus Infections -- virology KW - Precancerous Conditions -- surgery KW - Uterine Cervical Neoplasms -- surgery KW - Precancerous Conditions -- epidemiology KW - Precancerous Conditions -- virology KW - Papillomavirus Infections -- epidemiology KW - Cervical Intraepithelial Neoplasia -- surgery KW - Uterine Cervical Neoplasms -- epidemiology KW - Papillomaviridae -- isolation & purification KW - Cervical Intraepithelial Neoplasia -- virology KW - Electrosurgery KW - Papillomavirus Infections -- surgery KW - Uterine Cervical Neoplasms -- virology KW - Cervical Intraepithelial Neoplasia -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68422301?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.atitle=Viral+determinants+of+human+papillomavirus+persistence+following+loop+electrical+excision+procedure+treatment+for+cervical+intraepithelial+neoplasia+grade+2+or+3.&rft.au=Kreimer%2C+Aim%C3%A9e+R%3BKatki%2C+Hormuzd+A%3BSchiffman%2C+Mark%3BWheeler%2C+Cosette+M%3BCastle%2C+Philip+E%3BASCUS-LSIL+Triage+Study+Group&rft.aulast=Kreimer&rft.aufirst=Aim%C3%A9e&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Cancer+epidemiology%2C+biomarkers+%26+prevention+%3A+a+publication+of+the+American+Association+for+Cancer+Research%2C+cosponsored+by+the+American+Society+of+Preventive+Oncology&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-06-29 N1 - Date created - 2007-01-15 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Aerosolized red-tide toxins (brevetoxins) and asthma. AN - 68421030; 17218574 AB - With the increasing incidence of asthma, there is increasing concern over environmental exposures that may trigger asthma exacerbations. Blooms of the marine microalgae, Karenia brevis, cause red tides (or harmful algal blooms) annually throughout the Gulf of Mexico. K brevis produces highly potent natural polyether toxins, called brevetoxins, which are sodium channel blockers, and possibly histamine activators. In experimental animals, brevetoxins cause significant bronchoconstriction. In humans, a significant increase in self-reported respiratory symptoms has been described after recreational and occupational exposures to Florida red-tide aerosols, particularly among individuals with asthma. Before and after 1 h spent on beaches with and without an active K brevis red-tide exposure, 97 persons >or= 12 years of age with physician-diagnosed asthma were evaluated by questionnaire and spirometry. Concomitant environmental monitoring, water and air sampling, and personal monitoring for brevetoxins were performed. Participants were significantly more likely to report respiratory symptoms after K brevis red-tide aerosol exposure than before exposure. Participants demonstrated small, but statistically significant, decreases in FEV(1), midexpiratory phase of forced expiratory flow, and peak expiratory flow after exposure, particularly among those participants regularly using asthma medications. No significant differences were detected when there was no Florida red tide (ie, during nonexposure periods). This study demonstrated objectively measurable adverse changes in lung function from exposure to aerosolized Florida red-tide toxins in asthmatic subjects, particularly among those requiring regular therapy with asthma medications. Future studies will assess these susceptible subpopulations in more depth, as well as the possible long-term effects of these toxins. JF - Chest AU - Fleming, Lora E AU - Kirkpatrick, Barbara AU - Backer, Lorraine C AU - Bean, Judy A AU - Wanner, Adam AU - Reich, Andrew AU - Zaias, Julia AU - Cheng, Yung Sung AU - Pierce, Richard AU - Naar, Jerome AU - Abraham, William M AU - Baden, Daniel G AD - National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Sciences Center, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Miami, FL, USA. lfleming@med.miami.edu Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 187 EP - 194 VL - 131 IS - 1 SN - 0012-3692, 0012-3692 KW - Aerosols KW - 0 KW - Marine Toxins KW - Oxocins KW - brevetoxin KW - 98225-48-0 KW - Abridged Index Medicus KW - Index Medicus KW - Respiratory Function Tests KW - Mass Spectrometry KW - Animals KW - Humans KW - Aged KW - Child KW - Florida KW - Chromatography, High Pressure Liquid KW - Environmental Monitoring KW - Inhalation Exposure KW - Adult KW - Surveys and Questionnaires KW - Enzyme-Linked Immunosorbent Assay KW - Middle Aged KW - Adolescent KW - Male KW - Female KW - Asthma -- etiology KW - Dinoflagellida -- pathogenicity KW - Oxocins -- toxicity KW - Marine Toxins -- toxicity UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68421030?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Chest&rft.atitle=Aerosolized+red-tide+toxins+%28brevetoxins%29+and+asthma.&rft.au=Fleming%2C+Lora+E%3BKirkpatrick%2C+Barbara%3BBacker%2C+Lorraine+C%3BBean%2C+Judy+A%3BWanner%2C+Adam%3BReich%2C+Andrew%3BZaias%2C+Julia%3BCheng%2C+Yung+Sung%3BPierce%2C+Richard%3BNaar%2C+Jerome%3BAbraham%2C+William+M%3BBaden%2C+Daniel+G&rft.aulast=Fleming&rft.aufirst=Lora&rft.date=2007-01-01&rft.volume=131&rft.issue=1&rft.spage=187&rft.isbn=&rft.btitle=&rft.title=Chest&rft.issn=00123692&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-11-05 N1 - Date created - 2007-01-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Toxicon. 2000 Jul;38(7):981-93 [10728835] J Allergy Clin Immunol. 2005 Feb;115(2):213-9; quiz 220 [15696070] Environ Toxicol Chem. 2001 Jan;20(1):107-14 [11351396] Environ Health Perspect. 2002 Feb;110(2):179-85 [11836147] Respiration. 2002;69(1):38-45 [11844961] Environ Res. 2002 May;89(1):29-37 [12051782] Bull Environ Contam Toxicol. 2003 Jan;70(1):161-5 [12478439] Cell Mol Neurobiol. 2004 Aug;24(4):553-63 [15233378] JFMA. 1973 Nov;60(11):27-9 [4755462] J Allergy Clin Immunol. 1982 May;69(5):418-28 [7200498] Am J Public Health. 1991 Apr;81(4):471-4 [2003627] Am J Respir Crit Care Med. 1995 Sep;152(3):1107-36 [7663792] Annu Rev Public Health. 2005;26:89-113 [15760282] J Allergy Clin Immunol. 2005 Apr;115(4):689-99 [15805986] Environ Health Perspect. 2005 May;113(5):618-20 [15866773] Environ Health Perspect. 2005 May;113(5):626-31 [15866775] Environ Health Perspect. 2005 May;113(5):632-7 [15866776] Environ Health Perspect. 2005 May;113(5):638-43 [15866777] Environ Health Perspect. 2005 May;113(5):644-9 [15866778] Environ Health Perspect. 2005 May;113(5):650-7 [15866779] Environ Sci Technol. 2005 May 15;39(10):3443-9 [15954221] Am J Ind Med. 1997 Jun;31(6):671-7 [9131220] Child Care Health Dev. 2004 Nov;30(6):711-28 [15527481] Am J Respir Crit Care Med. 2005 Jan 1;171(1):26-34 [15447946] Int J Tuberc Lung Dis. 2000 Jul;4(7):633-8 [10907766] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Effects of selenium supplementation on expression of glutathione peroxidase isoforms in cultured human lung adenocarcinoma cell lines. AN - 68412532; 17052796 AB - Selenium is an essential nutrient, a component of several anti-oxidant enzymes, and a possible factor in cancer risk, including lung cancer. We determined the subtoxic range of selenium concentration (as sodium selenite) required to increase and maintain the expression of anti-oxidant selenoproteins gluthathione peroxidases GPX1 and GPX4 at a constant level in cultures of human lung adenocarcinoma cell lines (H460, H1703 and H1944) and in HPL1D, a non-transformed lung epithelial cell line. Selenium dose-dependently increased GPX1 protein expression 1.8-fold in HPL1D cells and approximately 40-fold in H460 and H1944 cancer cells, with maximum effects at 20-40 nM. GPX4 protein was also increased, but more so in HPL1D (five-fold) than in H460 or H1944 cells (two- to three-fold). GPX1 mRNA showed similar patterns but differences of lesser magnitude. GPX1 protein and activity level was not consistently detectable in H1703 cells, with or without Se supplementation; its mRNA was present but very low. GPX4 protein level was also low in H1703 cells, but was markedly increased by selenium supplementation (48-fold). These results confirm a role for selenium in risk of lung cancer and the independent regulation of GPX1 and GPX4. Characterization of individual tumors with regard to GPX1 and GPX4 levels and regulation might be useful for interpretation of clinical studies on effects of selenium in lung cancer risk. JF - Lung cancer (Amsterdam, Netherlands) AU - Romanowska, Malgorzata AU - Kikawa, Keith D AU - Fields, Janet R AU - Maciag, Anna AU - North, S Lynn AU - Shiao, Yih-Horng AU - Kasprzak, Kazimierz S AU - Anderson, Lucy M AD - Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, Building 538, Ft. Detrick, Frederick, MD 21702, USA. mromanowska@ncifcrf.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 35 EP - 42 VL - 55 IS - 1 SN - 0169-5002, 0169-5002 KW - RNA, Messenger KW - 0 KW - glutathione peroxidase GPX1 KW - EC 1.11.1.- KW - phospholipid-hydroperoxide glutathione peroxidase KW - EC 1.11.1.12 KW - Glutathione Peroxidase KW - EC 1.11.1.9 KW - Selenium KW - H6241UJ22B KW - Index Medicus KW - Gene Expression Regulation, Enzymologic -- drug effects KW - Humans KW - Cell Line, Tumor KW - RNA, Messenger -- genetics KW - Adenocarcinoma -- enzymology KW - Lung Neoplasms -- enzymology KW - Selenium -- pharmacology KW - Glutathione Peroxidase -- genetics KW - Gene Expression Regulation, Neoplastic -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68412532?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.atitle=Effects+of+selenium+supplementation+on+expression+of+glutathione+peroxidase+isoforms+in+cultured+human+lung+adenocarcinoma+cell+lines.&rft.au=Romanowska%2C+Malgorzata%3BKikawa%2C+Keith+D%3BFields%2C+Janet+R%3BMaciag%2C+Anna%3BNorth%2C+S+Lynn%3BShiao%2C+Yih-Horng%3BKasprzak%2C+Kazimierz+S%3BAnderson%2C+Lucy+M&rft.aulast=Romanowska&rft.aufirst=Malgorzata&rft.date=2007-01-01&rft.volume=55&rft.issue=1&rft.spage=35&rft.isbn=&rft.btitle=&rft.title=Lung+cancer+%28Amsterdam%2C+Netherlands%29&rft.issn=01695002&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-26 N1 - Date created - 2007-01-10 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Constitutive endocytosis of the metabotropic glutamate receptor mGluR7 is clathrin-independent. AN - 68410921; 16890965 AB - Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors (GPCRs) that are widely expressed throughout the brain and are involved in synaptic development, transmission, and plasticity. The endocytosis of several members of the GPCR superfamily of receptors, such as beta-adrenergic receptors, has been studied extensively. In contrast, the mechanisms regulating mGluR endocytosis and intracellular trafficking remain poorly defined. We describe here for the first time a distinct endocytic and intracellular sorting pathway utilized by mGluR7. We show that mGluR7 constitutively internalizes via a non-clathrin mediated pathway in heterologous cells and in neurons. Unlike clathrin-mediated NMDAR endocytosis, mGluR7 traffics via an Arf6-positive endosomal pathway, similar to other well-characterized proteins such as major histocompatibility complex class I (MHC I) and the GPI-anchored protein CD59. Thus constitutive endocytosis of mGluR7 in neurons is not regulated by clathrin-dependent mechanisms, and this clathrin-independent pathway ultimately determines the amount of receptor present on the plasma membrane available to bind and respond to glutamate. JF - Neuropharmacology AU - Lavezzari, Gabriela AU - Roche, Katherine W AD - National Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 35, Room 2C903, Bethesda, MD 20892, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 100 EP - 107 VL - 52 IS - 1 SN - 0028-3908, 0028-3908 KW - Antigens, CD95 KW - 0 KW - Clathrin KW - Histocompatibility Antigens Class I KW - Receptors, Metabotropic Glutamate KW - Transferrin KW - metabotropic glutamate receptor 7 KW - Sucrose KW - 57-50-1 KW - ADP-Ribosylation Factors KW - EC 3.6.5.2 KW - ADP-ribosylation factor 6 KW - Index Medicus KW - Animals KW - Histocompatibility Antigens Class I -- metabolism KW - Fluorescent Antibody Technique -- methods KW - Humans KW - Mutagenesis -- physiology KW - Endosomes KW - Transferrin -- metabolism KW - Rats KW - Transfection -- methods KW - Antigens, CD95 -- metabolism KW - Rats, Sprague-Dawley KW - Cells, Cultured KW - Sucrose -- pharmacology KW - Embryo, Mammalian KW - Neurons -- metabolism KW - Neurons -- drug effects KW - Endocytosis -- genetics KW - Clathrin -- physiology KW - Receptors, Metabotropic Glutamate -- metabolism KW - Hippocampus -- cytology KW - Endocytosis -- drug effects KW - Endocytosis -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68410921?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropharmacology&rft.atitle=Constitutive+endocytosis+of+the+metabotropic+glutamate+receptor+mGluR7+is+clathrin-independent.&rft.au=Lavezzari%2C+Gabriela%3BRoche%2C+Katherine+W&rft.aulast=Lavezzari&rft.aufirst=Gabriela&rft.date=2007-01-01&rft.volume=52&rft.issue=1&rft.spage=100&rft.isbn=&rft.btitle=&rft.title=Neuropharmacology&rft.issn=00283908&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-02 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Multivitamin and multimineral dietary supplements: definitions, characterization, bioavailability, and drug interactions. AN - 68409018; 17209208 AB - Although multivitamins, multiminerals, and similar terms (eg, multis or multiples) are commonly used, they have no standard scientific, regulatory, or marketplace definitions. Thus, multivitamins-multiminerals refers to products with widely varied compositions and characteristics. Multivitamin-multimineral composition databases use label values as surrogates for analyzed values. However, actual vitamin and mineral amounts often deviate from label values. Vitamin and mineral bioavailability for dietary supplements also lacks a standard scientific and regulatory definition and validated in vitro and animal models that accurately reflect human bioavailabilities. Systematic information on the bioavailability and bioequivalence of vitamins and minerals in marketed products and on potential drug interactions is scarce. Because of limited information on product characteristics, our ability to directly compare results across studies, estimate changes in usage patterns or intakes over time, and generalize from published results to marketed products is problematic. JF - The American journal of clinical nutrition AU - Yetley, Elizabeth A AD - Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892-7517, USA. yetleye@od.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 269S EP - 276S VL - 85 IS - 1 SN - 0002-9165, 0002-9165 KW - Minerals KW - 0 KW - Vitamins KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Evidence-Based Medicine KW - Primary Prevention KW - Consumer Product Safety KW - Risk Factors KW - Humans KW - Legislation, Drug KW - Biological Availability KW - Vitamins -- pharmacokinetics KW - Minerals -- pharmacokinetics KW - Drug Interactions KW - Dietary Supplements -- statistics & numerical data KW - Chronic Disease -- prevention & control KW - Vitamins -- adverse effects KW - Minerals -- administration & dosage KW - Dietary Supplements -- utilization KW - Vitamins -- administration & dosage KW - Minerals -- adverse effects KW - Dietary Supplements -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68409018?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+clinical+nutrition&rft.atitle=Multivitamin+and+multimineral+dietary+supplements%3A+definitions%2C+characterization%2C+bioavailability%2C+and+drug+interactions.&rft.au=Yetley%2C+Elizabeth+A&rft.aulast=Yetley&rft.aufirst=Elizabeth&rft.date=2007-01-01&rft.volume=85&rft.issue=1&rft.spage=269S&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+clinical+nutrition&rft.issn=00029165&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Clinical trials of vitamin and mineral supplements for cancer prevention. AN - 68408730; 17209217 AB - Approximately 20-30% of Americans consume multivitamin supplements daily, indicating high public interest in the prevention of cancer and other chronic diseases through a nutrition-based approach. Although several bioactive food components, including vitamins and minerals, have been investigated for their ability to affect cancer risk, few large, randomized, placebo-controlled clinical trials of multivitamins with cancer as the primary endpoint have been performed. The results of most large-scale trials of multivitamin supplements (combinations of > or = 2 vitamins and minerals) to prevent cancer have been mixed. The Linxian General Population and Dysplasia trials found a decreased risk of cancer, particularly stomach cancer, for participants taking a multivitamin supplement, but this was in a borderline-deficient population in China. Two trials, the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study and the beta-Carotene and Retinol Efficacy Trial, found an increased risk of lung cancer among male cigarette smokers or asbestos-exposed persons taking beta-carotene-a surprising result, considering that most epidemiologic studies have suggested that consumption of fruit and vegetables appears to lower cancer risk. To clarify the effects of multivitamin supplements, several large randomized clinical trials are underway, including the Physicians' Health Study II, the Selenium and Vitamin E Cancer Prevention Trial, and a European study, Supplémentation en Vitamines et Minéraux Antioxydants (SU.VI. MAX). Because epidemiologic studies generally evaluate foods rather than specific bioactive food components, a systematic approach to determining how combinations of vitamins and minerals may interact to ameliorate cancer risk is necessary to further our understanding of the potential benefits and risks of supplement use. JF - The American journal of clinical nutrition AU - Greenwald, Peter AU - Anderson, Darrell AU - Nelson, Stefanie A AU - Taylor, Philip R AD - Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-7309, USA. pg37g@nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 314S EP - 317S VL - 85 IS - 1 SN - 0002-9165, 0002-9165 KW - Minerals KW - 0 KW - Vitamins KW - Abridged Index Medicus KW - Index Medicus KW - Randomized Controlled Trials as Topic KW - Evidence-Based Medicine KW - Humans KW - Dietary Supplements KW - Risk Assessment KW - Vitamins -- adverse effects KW - Minerals -- administration & dosage KW - Vitamins -- administration & dosage KW - Minerals -- adverse effects KW - Neoplasms -- prevention & control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68408730?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+clinical+nutrition&rft.atitle=Clinical+trials+of+vitamin+and+mineral+supplements+for+cancer+prevention.&rft.au=Greenwald%2C+Peter%3BAnderson%2C+Darrell%3BNelson%2C+Stefanie+A%3BTaylor%2C+Philip+R&rft.aulast=Greenwald&rft.aufirst=Peter&rft.date=2007-01-01&rft.volume=85&rft.issue=1&rft.spage=314S&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+clinical+nutrition&rft.issn=00029165&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - National Institutes of Health State-of-the-Science Conference Statement: multivitamin/mineral supplements and chronic disease prevention. AN - 68408603; 17209206 JF - The American journal of clinical nutrition AU - National Institutes of Health State-of-the-Science Panel AD - National Institutes of Health State-of-the-Science Panel Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 257S EP - 264S VL - 85 IS - 1 SN - 0002-9165, 0002-9165 KW - Minerals KW - 0 KW - Vitamins KW - Abridged Index Medicus KW - Index Medicus KW - United States KW - Evidence-Based Medicine KW - Primary Prevention KW - Risk Factors KW - Humans KW - Dietary Supplements -- statistics & numerical data KW - Chronic Disease -- prevention & control KW - Vitamins -- adverse effects KW - Minerals -- administration & dosage KW - Dietary Supplements -- utilization KW - Vitamins -- administration & dosage KW - Minerals -- adverse effects KW - Dietary Supplements -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68408603?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+clinical+nutrition&rft.atitle=National+Institutes+of+Health+State-of-the-Science+Conference+Statement%3A+multivitamin%2Fmineral+supplements+and+chronic+disease+prevention.&rft.au=National+Institutes+of+Health+State-of-the-Science+Panel&rft.aulast=National+Institutes+of+Health+State-of-the-Science+Panel&rft.aufirst=&rft.date=2007-01-01&rft.volume=85&rft.issue=1&rft.spage=257S&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+clinical+nutrition&rft.issn=00029165&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - PET [11C]DASB imaging of serotonin transporters in patients with alcoholism. AN - 68408566; 17207098 AB - Alcoholism and aggression have each been associated with neurochemical measurements suggestive of decreased serotonin synaptic transmission. We measured densities of the serotonin transporter (SERT) in a moderate-sized sample of alcoholic patients who were assessed for aggressive characteristics. Thirty alcoholic inpatients and 18 healthy controls received a PET scan with [(11)C]-3-amino-4-(2-dimethylaminomethylphenylsulfanyl)-benzonitrile. The alcoholic inpatients were classified as aggressive or nonaggressive based on a comparison between the top third and bottom third scores on the Buss-Durkee Hostility Index. Using a pixel-wise comparison, no brain region showed significant alterations in SERT binding among the 3 groups of subjects (aggressive alcoholic subjects, nonaggressive alcoholic subjects, and healthy controls) or between the combined alcoholic group and healthy controls. None of the clinical measures (including measures of aggression) correlated with SERT binding in the alcoholic subjects. Contrary to prior imaging reports using the nonselective ligand [(123)I]beta-CIT, we found no significant alterations of SERT density in alcoholic patients. JF - Alcoholism, clinical and experimental research AU - Brown, Amira K AU - George, David T AU - Fujita, Masahiro AU - Liow, Jeih-San AU - Ichise, Masanori AU - Hibbeln, Joseph AU - Ghose, Subroto AU - Sangare, Janet AU - Hommer, Daniel AU - Innis, Robert B AD - Molecular Imaging Branch, National Institute of Mental Health, One Center Drive, Bethesda, MD 20892, USA. amirabrown@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 28 EP - 32 VL - 31 IS - 1 SN - 0145-6008, 0145-6008 KW - 3-amino-4-(2-dimethylaminomethylphenylsulfanyl)benzonitrile KW - 0 KW - Aniline Compounds KW - Radiopharmaceuticals KW - Serotonin Plasma Membrane Transport Proteins KW - Sulfides KW - Index Medicus KW - Neocortex -- metabolism KW - Genotype KW - Brain Mapping KW - Psychiatric Status Rating Scales KW - Positron-Emission Tomography KW - Aggression -- psychology KW - Humans KW - Adult KW - Smoking -- metabolism KW - Data Interpretation, Statistical KW - Neocortex -- diagnostic imaging KW - Image Processing, Computer-Assisted KW - Male KW - Female KW - Serotonin Plasma Membrane Transport Proteins -- metabolism KW - Alcoholism -- diagnostic imaging KW - Alcoholism -- metabolism KW - Alcoholism -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68408566?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=PET+%5B11C%5DDASB+imaging+of+serotonin+transporters+in+patients+with+alcoholism.&rft.au=Brown%2C+Amira+K%3BGeorge%2C+David+T%3BFujita%2C+Masahiro%3BLiow%2C+Jeih-San%3BIchise%2C+Masanori%3BHibbeln%2C+Joseph%3BGhose%2C+Subroto%3BSangare%2C+Janet%3BHommer%2C+Daniel%3BInnis%2C+Robert+B&rft.aulast=Brown&rft.aufirst=Amira&rft.date=2007-01-01&rft.volume=31&rft.issue=1&rft.spage=28&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-06 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Impact of age at first drink on stress-reactive drinking. AN - 68408464; 17207104 AB - Although recent data from animal models indicate that adolescent ethanol exposure increases self-administered ethanol intake in adult rats, the impact of age at first drink on the association between stress and drinking has not been studied in humans. Data collected in the 2001 to 2002 National Epidemiologic Survey on Alcohol and Related Conditions (NESARC) were used to estimate the extent to which age at first drink modified the association between stress and average daily volume (ADV) of ethanol intake in a sample of 26,946 past-year drinkers. Successive models estimated the magnitude and significance of the interaction between age at first drink (ages 14 or younger, 15-17, and 18 or older) and number of stressors (out of 12 past-year negative life events) after (1) adjusting for sociodemographic characteristics, (2) additionally adjusting for family history of alcoholism, comorbid psychopathology, adolescent, and past-year tobacco and illicit drug use, and (3) additionally adjusting for all other significant interactions with number of stressors. Even after adjusting for a wide range of confounders and their interactions with stress, initiation of drinking at ages 14 and younger increased the association between the number of stressors and ADV of ethanol consumption by 8% (p=0.014), when considering the full range of 12 potential stressors. In fact, the positive association between stress and consumption was significant only for this group of drinkers with early adolescent exposure to ethanol. Within this group, ADV of consumption increased by an average of 7% with each additional stressor experienced, although the exact percentage increase varied as a function of other covariates that had significant interactions with stress. When a reduced set of 4 stressors was considered, the magnitudes of the associations were mostly unchanged, but the modifying effect of age at first drink fell short of statistical significance (p=0.309) in the fully adjusted model. The findings of this study are consistent with the argument that early-onset drinking may increase stress-reactive ethanol consumption; however, these findings need to be replicated in an experimental human study in order to control fully the direction of the relationship between stress and consumption. JF - Alcoholism, clinical and experimental research AU - Dawson, Deborah A AU - Grant, Bridget F AU - Li, Ting-Kai AD - Laboratory of Epidemiology and Biometry, Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5635 Fishers Lane MSC 9304, Bethesda, MD 20892, USA. ddawson@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 69 EP - 77 VL - 31 IS - 1 SN - 0145-6008, 0145-6008 KW - Index Medicus KW - Age of Onset KW - Tobacco Use Disorder -- epidemiology KW - Humans KW - Diagnosis, Dual (Psychiatry) KW - Aged KW - Mental Disorders -- psychology KW - Substance-Related Disorders -- psychology KW - Alcoholism -- genetics KW - Smoking -- epidemiology KW - Psychiatric Status Rating Scales KW - Aged, 80 and over KW - Adult KW - Tobacco Use Disorder -- psychology KW - Middle Aged KW - Adolescent KW - Mental Disorders -- complications KW - Female KW - Male KW - Substance-Related Disorders -- epidemiology KW - Alcohol Drinking -- psychology KW - Stress, Psychological -- epidemiology KW - Alcohol Drinking -- epidemiology KW - Stress, Psychological -- psychology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68408464?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcoholism%2C+clinical+and+experimental+research&rft.atitle=Impact+of+age+at+first+drink+on+stress-reactive+drinking.&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BLi%2C+Ting-Kai&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2007-01-01&rft.volume=31&rft.issue=1&rft.spage=69&rft.isbn=&rft.btitle=&rft.title=Alcoholism%2C+clinical+and+experimental+research&rft.issn=01456008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-06 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - In situ structural characterization of glycerophospholipids and sulfatides in brain tissue using MALDI-MS/MS. AN - 68407960; 17005416 AB - Lipids are major structural components of biomembranes. Negatively charged species such as phosphatidylinositol, phosphatidylserine, sulfatides, and the zwitterionic phosphatidylethanolamines are major components of the cytoplasmic surface of the cellular membrane lipid bilayer and play a key role in several receptors signaling functions. Lipids are not just involved in metabolic and neurological diseases; negatively charged lipids in particular play crucial roles in physiological events such as signal transduction, receptors, and enzymatic activation, as well as storage and release of therapeutic drugs and toxic chemicals in the body. Due to the importance of their role in signaling, the field of lipidomics has rapidly expanded in recent years. In the present study, direct probing of tissue slices with negative ion mode matrix assisted laser desorption/ionization mass spectrometry was employed to profile the distribution of lipids in the brain. In total, 32 lipid species consisting of phosphatidylethanolamines, phosphatidylglycerol, phosphatidylinositols, phosphatidylserines, and sulfatides were assigned. To confirm the structure of lipid species, MALDI-MS/MS analysis was conducted. Product-ion spectra obtained in negative ion mode allow for the assignment of the head groups and the fatty acid chains for the lipid species. JF - Journal of the American Society for Mass Spectrometry AU - Jackson, Shelley N AU - Wang, Hay-Yan J AU - Woods, Amina S AD - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland 21224, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 17 EP - 26 VL - 18 IS - 1 SN - 1044-0305, 1044-0305 KW - Glycerophospholipids KW - 0 KW - Sulfoglycosphingolipids KW - Index Medicus KW - Rats KW - Animals KW - Rats, Sprague-Dawley KW - Cerebellum -- chemistry KW - Male KW - Glycerophospholipids -- chemistry KW - Sulfoglycosphingolipids -- chemistry KW - Brain Chemistry KW - Brain KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68407960?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+American+Society+for+Mass+Spectrometry&rft.atitle=In+situ+structural+characterization+of+glycerophospholipids+and+sulfatides+in+brain+tissue+using+MALDI-MS%2FMS.&rft.au=Jackson%2C+Shelley+N%3BWang%2C+Hay-Yan+J%3BWoods%2C+Amina+S&rft.aulast=Jackson&rft.aufirst=Shelley&rft.date=2007-01-01&rft.volume=18&rft.issue=1&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+American+Society+for+Mass+Spectrometry&rft.issn=10440305&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-02 N1 - Date created - 2007-01-08 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Analysis of mouse development with conditional mutagenesis. AN - 68405524; 17203658 AB - Explorations into the molecular embryology of the mouse have played a vital role in our understanding of the basic mechanisms of gene regulation that govern development and disease. In the last 15 years, these mechanisms have been analyzed with vastly greater precision and clarity with the advent of systems that allow the conditional control of gene expression. Typically, this control is achieved by silencing or activating the gene of interest with site-specific DNA recombination or transcriptional transactivation. In this review, I discuss the application of these technologies to mouse development, focusing on recent innovations and experimental designs that specifically aid the study of the mouse embryo. JF - Handbook of experimental pharmacology AU - Lewandoski, M AD - Laboratory of Cancer and Developmental Biology, NCI-Frederick, National Institutes of Health, Frederick, MD 21702-1201, USA. mlewandoski@mail.ncifcrf.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 235 EP - 262 IS - 178 SN - 0171-2004, 0171-2004 KW - Recombinases KW - 0 KW - Index Medicus KW - Animals KW - RNA Interference KW - Mice, Transgenic KW - Gene Targeting KW - Recombinases -- metabolism KW - Mice -- embryology KW - Mutagenesis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68405524?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Handbook+of+experimental+pharmacology&rft.atitle=Analysis+of+mouse+development+with+conditional+mutagenesis.&rft.au=Lewandoski%2C+M&rft.aulast=Lewandoski&rft.aufirst=M&rft.date=2007-01-01&rft.volume=&rft.issue=178&rft.spage=235&rft.isbn=&rft.btitle=&rft.title=Handbook+of+experimental+pharmacology&rft.issn=01712004&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-17 N1 - Date created - 2007-01-05 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Regulation of the mouse alphaB-crystallin and MKBP/HspB2 promoter activities by shared and gene specific intergenic elements: the importance of context dependency. AN - 68395629; 17939115 AB - The closely linked (863 bp), divergently arranged mouse myotonic dystrophy kinase binding protein (Mkbp)/HspB2 and small heat shock protein (shsp)/alphaB-crystallin genes have different patterns of tissue-specific expression. We showed previously that an intergenic enhancing region (-436/-257 relative to alphaB-crystallin transcription start site) selectively activates the alphaB-crystallin promoter in an orientation-dependent manner (Swamynathan, S.K. and J. Piatigorsky 2002. J. Biol. Chem. 277:49700-6). Here we show that cis-elements alphaBE1 (-420/-396) and alphaBE3 (-320/-300) functionally interact with glucocorticoid receptor (GR) and Sp1, respectively, both in vitro and in vivo. alphaBE1:GR regulates both the HspB2 and alphaB-crystallin promoters, while alphaBE3:Sp1 selectively regulates the alphaB-crystallin promoter, as judged by mutagenesis and co-transfection tests. Enhancer blocking assays indicate that the -836/-622 fragment can act as a negative regulator in transfection tests, raising the possibility that it contributes to the differential expression of the proximal HspB2 promoter and distal alphaB-crystallin promoter. Finally, experiments utilizing transiently transfected cells and transgenic mice show that two conserved E-box elements (-726/-721 and -702/-697) bind nuclear proteins and differentially regulate the HspB2 and alphaB-crystallin promoters in a tissue-specific manner. Taken together, our results indicate that the linked, differentially expressed HspB2 and alphaB-crystallin genes have evolved shared and promoter-preferred cis-control elements within the intergenic sequence. The context-dependency of cis-elements provides multiple opportunities for evolutionary novelty by small sequence changes. JF - The International journal of developmental biology AU - Swamynathan, Shivalingappa K AU - Piatigorsky, Joram AD - Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, Maryland 20892, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 689 EP - 700 VL - 51 IS - 8 SN - 0214-6282, 0214-6282 KW - HSP27 Heat-Shock Proteins KW - 0 KW - Heat-Shock Proteins KW - Hspb2 protein, mouse KW - Sp1 Transcription Factor KW - Transcription Factors KW - alpha-Crystallin B Chain KW - Index Medicus KW - Animals KW - Chickens KW - Base Sequence KW - Transcription Factors -- metabolism KW - Humans KW - DNA Mutational Analysis KW - Sp1 Transcription Factor -- metabolism KW - Molecular Sequence Data KW - Mice KW - Tissue Distribution KW - K562 Cells KW - Mutagenesis KW - alpha-Crystallin B Chain -- biosynthesis KW - Promoter Regions, Genetic KW - Heat-Shock Proteins -- biosynthesis KW - Gene Expression Regulation KW - alpha-Crystallin B Chain -- genetics KW - Heat-Shock Proteins -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68395629?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+International+journal+of+developmental+biology&rft.atitle=Regulation+of+the+mouse+alphaB-crystallin+and+MKBP%2FHspB2+promoter+activities+by+shared+and+gene+specific+intergenic+elements%3A+the+importance+of+context+dependency.&rft.au=Swamynathan%2C+Shivalingappa+K%3BPiatigorsky%2C+Joram&rft.aulast=Swamynathan&rft.aufirst=Shivalingappa&rft.date=2007-01-01&rft.volume=51&rft.issue=8&rft.spage=689&rft.isbn=&rft.btitle=&rft.title=The+International+journal+of+developmental+biology&rft.issn=02146282&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-01-22 N1 - Date created - 2007-10-16 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. AN - 68394858; 17180163 AB - Mounting evidence indicates that microglial activation contributes to neuronal damage in neurodegenerative diseases. Recent studies show that in response to certain environmental toxins and endogenous proteins, microglia can enter an overactivated state and release reactive oxygen species (ROS) that cause neurotoxicity. Pattern recognition receptors expressed on the microglial surface seem to be one of the primary, common pathways by which diverse toxin signals are transduced into ROS production. Overactivated microglia can be detected using imaging techniques and therefore this knowledge offers an opportunity not only for early diagnosis but, importantly, for the development of targeted anti-inflammatory therapies that might slow or halt the progression of neurodegenerative disease. JF - Nature reviews. Neuroscience AU - Block, Michelle L AU - Zecca, Luigi AU - Hong, Jau-Shyong AD - Neuropharmacology Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. Block@niehs.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 57 EP - 69 VL - 8 IS - 1 SN - 1471-003X, 1471-003X KW - Reactive Oxygen Species KW - 0 KW - Index Medicus KW - Reactive Oxygen Species -- metabolism KW - Neurodegenerative Diseases -- genetics KW - Animals KW - Humans KW - Neurodegenerative Diseases -- metabolism KW - Neurodegenerative Diseases -- pathology KW - Microglia -- physiology KW - Neurotoxicity Syndromes -- metabolism KW - Neurotoxicity Syndromes -- genetics KW - Neurotoxicity Syndromes -- pathology KW - Microglia -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68394858?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+reviews.+Neuroscience&rft.atitle=Microglia-mediated+neurotoxicity%3A+uncovering+the+molecular+mechanisms.&rft.au=Block%2C+Michelle+L%3BZecca%2C+Luigi%3BHong%2C+Jau-Shyong&rft.aulast=Block&rft.aufirst=Michelle&rft.date=2007-01-01&rft.volume=8&rft.issue=1&rft.spage=57&rft.isbn=&rft.btitle=&rft.title=Nature+reviews.+Neuroscience&rft.issn=1471003X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-06 N1 - Date created - 2006-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Pegylated arginine deiminase lowers hepatitis C viral titers and inhibits nitric oxide synthesis. AN - 68393925; 17201887 AB - The arginine-degrading enzyme, arginine deiminase conjugated to polyethylene glycol (ADI-SS PEG 20,000 mw), reduces extracellular arginine, has minimal toxicity, decreases tumor burden and improves liver function in patients with chronic hepatitis C virus infection (HCV) and inoperable hepatocellular carcinoma (HCC). Reduced extracellular arginine inhibits viral replication through unknown mechanisms. It is hypothesized that ADI-SS PEG 20,000 mw reduces HCV viral titers through nitric oxide (NO)-dependent effects. The effects of ADI-SS PEG 20,000 mw (dose, 160 IU/m2; three cycles of four once-weekly i.m. injections) on HCV titers, serum NO and plasma arginine, were evaluated using archived plasma from patients with HCC and HCV and in vitro cell model measurements of HCV replication. ADI-SS PEG 20,000 mw selectively inhibited HCV replication in vitro (IC50 = 0.027 IU/mL). Fifteen HCC/HCV patients completed treatment. The HCV titers were reduced by up to 99% in five out of 10 (50%) HCV-serotype 1b patients (P = 0.0093). These patients also experienced significant improvements in liver function (P = 0.0091). There were concomitant reductions of plasma arginine and serum NO levels. The HCV titer was not reduced in HCV-type 2c patients. Reduction of extracellular arginine by ADI-SS PEG 20,000 mw in HCC patients reduces HCV viral titers and improves liver function, possibly through suppression of NO. JF - Journal of gastroenterology and hepatology AU - Izzo, Francesco AU - Montella, Maurizio AU - Orlando, Antonio Pio AU - Nasti, Guglielmo AU - Beneduce, Gerardo AU - Castello, Giuseppe AU - Cremona, Francesco AU - Ensor, C Mark AU - Holtzberg, Frederick W AU - Bomalaski, John S AU - Clark, Mike A AU - Curley, Steven A AU - Orlando, Raffaele AU - Scordino, Fabrizio AU - Korba, Brent E AD - National Cancer Institute G Pascale Foundation, Naples, Italy. izzo@connect.it Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 86 EP - 91 VL - 22 IS - 1 SN - 0815-9319, 0815-9319 KW - RNA, Viral KW - 0 KW - Polyethylene Glycols KW - 30IQX730WE KW - Nitric Oxide KW - 31C4KY9ESH KW - Arginine KW - 94ZLA3W45F KW - Hydrolases KW - EC 3.- KW - arginine deiminase KW - EC 3.5.3.6 KW - Index Medicus KW - Carcinoma, Hepatocellular -- virology KW - Tomography, Spiral Computed KW - Carcinoma, Hepatocellular -- diagnostic imaging KW - Carcinoma, Hepatocellular -- drug therapy KW - Liver Neoplasms -- drug therapy KW - Humans KW - Adult KW - Liver Neoplasms -- virology KW - Liver Neoplasms -- diagnostic imaging KW - Polyethylene Glycols -- pharmacology KW - Statistics, Nonparametric KW - RNA, Viral -- analysis KW - Hydrolases -- pharmacology KW - Nitric Oxide -- blood KW - Arginine -- blood KW - Nitric Oxide -- biosynthesis KW - Hepacivirus -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68393925?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+gastroenterology+and+hepatology&rft.atitle=Pegylated+arginine+deiminase+lowers+hepatitis+C+viral+titers+and+inhibits+nitric+oxide+synthesis.&rft.au=Izzo%2C+Francesco%3BMontella%2C+Maurizio%3BOrlando%2C+Antonio+Pio%3BNasti%2C+Guglielmo%3BBeneduce%2C+Gerardo%3BCastello%2C+Giuseppe%3BCremona%2C+Francesco%3BEnsor%2C+C+Mark%3BHoltzberg%2C+Frederick+W%3BBomalaski%2C+John+S%3BClark%2C+Mike+A%3BCurley%2C+Steven+A%3BOrlando%2C+Raffaele%3BScordino%2C+Fabrizio%3BKorba%2C+Brent+E&rft.aulast=Izzo&rft.aufirst=Francesco&rft.date=2007-01-01&rft.volume=22&rft.issue=1&rft.spage=86&rft.isbn=&rft.btitle=&rft.title=Journal+of+gastroenterology+and+hepatology&rft.issn=08159319&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-15 N1 - Date created - 2007-01-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Stimulant-induced enhanced sexual desire as a potential contributing factor in HIV transmission. AN - 68392326; 17202559 AB - Stimulant abuse is associated with an increased risk of contracting human immunodeficiency virus (HIV). Although sharing of contaminated needles is one of the routes by which HIV is spread, noninjection abusers are also at high risk. The authors investigated the effect of the stimulant drug methylphenidate (given intravenously) on sexual desire as a possible contributing factor to risky sexual behavior associated with the contraction of HIV. The effects of intravenous methylphenidate (0.5 mg/kg) on self-reports of sexual desire (rated from 0-10) were evaluated in 39 comparison subjects and 39 cocaine abusers. Intravenous methylphenidate significantly increased self-reports of sexual desire in comparison subjects (1.4 versus 3.7) and cocaine abusers (2.8 versus 4.8). Stimulant-induced enhancement of sexual desire could be one mechanism by which stimulant drugs such as cocaine and methamphetamine increase the risk for HIV transmission even when they are not injected. JF - The American journal of psychiatry AU - Volkow, Nora D AU - Wang, Gene-Jack AU - Fowler, Joanna S AU - Telang, Frank AU - Jayne, Millard AU - Wong, Christopher AD - National Institute on Drug Abuse, 6001 Executive Blvd., Rm. 5274, Bethesda, MD 20892, USA. nvolkow@nida.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 157 EP - 160 VL - 164 IS - 1 SN - 0002-953X, 0002-953X KW - Central Nervous System Stimulants KW - 0 KW - Placebos KW - Methylphenidate KW - 207ZZ9QZ49 KW - Methamphetamine KW - 44RAL3456C KW - Abridged Index Medicus KW - Index Medicus KW - Administration, Oral KW - Risk-Taking KW - Injections, Intravenous KW - Dose-Response Relationship, Drug KW - Methamphetamine -- adverse effects KW - Humans KW - Adult KW - Methamphetamine -- pharmacology KW - Male KW - Female KW - Comorbidity KW - Central Nervous System Stimulants -- pharmacology KW - Methylphenidate -- administration & dosage KW - HIV Infections -- transmission KW - Methylphenidate -- pharmacology KW - Cocaine-Related Disorders -- psychology KW - Cocaine-Related Disorders -- epidemiology KW - Central Nervous System Stimulants -- administration & dosage KW - Central Nervous System Stimulants -- adverse effects KW - Cocaine-Related Disorders -- complications KW - Libido -- drug effects KW - Methylphenidate -- adverse effects KW - Sexual Behavior -- psychology KW - HIV Infections -- epidemiology KW - HIV Infections -- psychology KW - Sexual Behavior -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68392326?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+journal+of+psychiatry&rft.atitle=Stimulant-induced+enhanced+sexual+desire+as+a+potential+contributing+factor+in+HIV+transmission.&rft.au=Volkow%2C+Nora+D%3BWang%2C+Gene-Jack%3BFowler%2C+Joanna+S%3BTelang%2C+Frank%3BJayne%2C+Millard%3BWong%2C+Christopher&rft.aulast=Volkow&rft.aufirst=Nora&rft.date=2007-01-01&rft.volume=164&rft.issue=1&rft.spage=157&rft.isbn=&rft.btitle=&rft.title=The+American+journal+of+psychiatry&rft.issn=0002953X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-12 N1 - Date created - 2007-01-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. AN - 68388065; 17200346 AB - CLIC4, a member of a family of intracellular chloride channels, is regulated by p53, c-Myc, and tumor necrosis factor-alpha. Regulation by factors involved in cancer pathogenesis, together with the previously shown proapoptotic activity of CLIC4, suggests that the protein may have a tumor suppressor function. To address this possibility, we characterized the expression profile, subcellular localization, and gene integrity of CLIC4 in human cancers and determined the functional consequences of CLIC4 expression in tumor epithelium and stromal cells. CLIC4 expression profiles were analyzed by genomics, proteomics, bioinformatics, and tissue microarrays. CLIC4 expression, as a consequence of crosstalk between stroma and epithelium, was tested in vitro by coculture of breast epithelial tumor cells and normal fibroblasts, and the functional consequences of CLIC4 expression was tested in vivo in xenografts of human breast tumor cell lines reconstituted with CLIC4 or mixed with fibroblasts that overexpress CLIC4 transgenically. In cDNA arrays of matched human normal and tumor tissues, CLIC4 expression was reduced in renal, ovarian, and breast cancers. However, CLIC4 protein levels were variable in tumor lysate arrays. Transcript sequences of CLIC4 from the human expressed sequence tag database and manual sequencing of cDNA from 60 human cancer cell lines (NCI60) failed to reveal deletion or mutations in the CLIC4 gene. On matched tissue arrays, CLIC4 was predominantly nuclear in normal human epithelial tissues but not cancers. With advancing malignant progression, CLIC4 staining became undetectable in tumor cells, but expression increased in stromal cells coincident with up-regulation of alpha-smooth muscle actin, suggesting that CLIC4 is up-regulated in myofibroblasts. Coculture of cancer cells and fibroblasts induced the expression of both CLIC4 and alpha-smooth muscle actin in fibroblasts adjacent to tumor nests. Introduction of CLIC4 or nuclear targeted CLIC4 via adenovirus into human breast cancer xenografts inhibited tumor growth, whereas overexpression of CLIC4 in stromal cells of xenografts enhanced tumor growth. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth. JF - Clinical cancer research : an official journal of the American Association for Cancer Research AU - Suh, Kwang S AU - Crutchley, John M AU - Koochek, Arash AU - Ryscavage, Andrew AU - Bhat, Kiran AU - Tanaka, Takemi AU - Oshima, Akira AU - Fitzgerald, Peter AU - Yuspa, Stuart H AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA. Y1 - 2007/01/01/ PY - 2007 DA - 2007 Jan 01 SP - 121 EP - 131 VL - 13 IS - 1 SN - 1078-0432, 1078-0432 KW - Actins KW - 0 KW - CLIC4 protein, human KW - Chloride Channels KW - Proto-Oncogene Proteins c-myc KW - TP53 protein, human KW - Tumor Necrosis Factor-alpha KW - Tumor Suppressor Protein p53 KW - Index Medicus KW - Animals KW - Genes, Tumor Suppressor KW - DNA Mutational Analysis KW - Humans KW - Disease Progression KW - Actins -- metabolism KW - Cell Line, Tumor KW - Mice KW - Proto-Oncogene Proteins c-myc -- metabolism KW - Tumor Suppressor Protein p53 -- metabolism KW - Fibroblasts -- metabolism KW - Neoplasm Transplantation KW - Epithelium -- metabolism KW - Tumor Necrosis Factor-alpha -- metabolism KW - Gene Expression Regulation, Neoplastic KW - Neoplasms -- pathology KW - Chloride Channels -- metabolism KW - Up-Regulation KW - Chloride Channels -- genetics KW - Neoplasms -- genetics KW - Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68388065?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.atitle=Reciprocal+modifications+of+CLIC4+in+tumor+epithelium+and+stroma+mark+malignant+progression+of+multiple+human+cancers.&rft.au=Suh%2C+Kwang+S%3BCrutchley%2C+John+M%3BKoochek%2C+Arash%3BRyscavage%2C+Andrew%3BBhat%2C+Kiran%3BTanaka%2C+Takemi%3BOshima%2C+Akira%3BFitzgerald%2C+Peter%3BYuspa%2C+Stuart+H&rft.aulast=Suh&rft.aufirst=Kwang&rft.date=2007-01-01&rft.volume=13&rft.issue=1&rft.spage=121&rft.isbn=&rft.btitle=&rft.title=Clinical+cancer+research+%3A+an+official+journal+of+the+American+Association+for+Cancer+Research&rft.issn=10780432&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-12 N1 - Date created - 2007-01-03 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Proteome and antigen profiling of Coxiella burnetii developmental forms. AN - 68386736; 17088354 AB - A biphasic developmental cycle whereby highly resistant small-cell variants (SCVs) are generated from large-cell variants (LCVs) is considered fundamental to the virulence of Coxiella burnetii, the causative agent of human Q fever. In this study a proteome analysis of C. burnetii developmental forms was conducted to provide insight into their unique biological and immunological properties. Silver-stained gels of SCV and LCV lysates separated by two-dimensional (2-D) gel electrophoresis resolved over 675 proteins in both developmental forms. Forty-eight proteins were greater than twofold more abundant in LCVs than in SCVs, with six proteins greater than twofold more abundant in SCVs than in LCVs. Four and 15 upregulated proteins of SCVs and LCVs, respectively, were identified by mass spectrometry, and their predicted functional roles are consistent with a metabolically active LCV and a structurally resistant SCV. One-dimensional and 2-D immunoblots of cell form lysates probed with sera from infected/vaccinated guinea pigs and convalescent-phase serum from human patients who had recovered from acute Q fever, respectively, revealed both unique SCV/LCV antigens and common SCV/LCV antigens that were often differentially synthesized. Antigens recognized during human infection were identified by mass spectroscopy and included both previously described immunodominant proteins of C. burnetii and novel immunogenic proteins that may be important in the pathophysiology of clinical Q fever and/or the induction of protective immunity. JF - Infection and immunity AU - Coleman, Sherry A AU - Fischer, Elizabeth R AU - Cockrell, Diane C AU - Voth, Daniel E AU - Howe, Dale AU - Mead, David J AU - Samuel, James E AU - Heinzen, Robert A AD - Coxiella Pathogenesis Section, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 290 EP - 298 VL - 75 IS - 1 SN - 0019-9567, 0019-9567 KW - Antigens, Bacterial KW - 0 KW - Bacterial Proteins KW - Immunodominant Epitopes KW - Proteome KW - Index Medicus KW - Immunodominant Epitopes -- immunology KW - Microscopy, Electron, Transmission KW - Mass Spectrometry KW - Immunoblotting KW - Comet Assay KW - Animals KW - Guinea Pigs KW - Humans KW - Electrophoresis, Gel, Two-Dimensional KW - Coxiella burnetii -- physiology KW - Bacterial Proteins -- immunology KW - Antigens, Bacterial -- analysis KW - Bacterial Proteins -- analysis KW - Antigens, Bacterial -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68386736?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+immunity&rft.atitle=Proteome+and+antigen+profiling+of+Coxiella+burnetii+developmental+forms.&rft.au=Coleman%2C+Sherry+A%3BFischer%2C+Elizabeth+R%3BCockrell%2C+Diane+C%3BVoth%2C+Daniel+E%3BHowe%2C+Dale%3BMead%2C+David+J%3BSamuel%2C+James+E%3BHeinzen%2C+Robert+A&rft.aulast=Coleman&rft.aufirst=Sherry&rft.date=2007-01-01&rft.volume=75&rft.issue=1&rft.spage=290&rft.isbn=&rft.btitle=&rft.title=Infection+and+immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-30 N1 - Date created - 2006-12-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Mol Microbiol. 2005 May;56(3):719-34 [15819627] Crit Rev Biochem Mol Biol. 2004 Sep-Dec;39(5-6):261-77 [15763705] Curr Protein Pept Sci. 2005 Dec;6(6):559-66 [16381604] Plasmid. 2006 Mar;55(2):135-44 [16229890] J Bacteriol. 2006 Apr;188(7):2309-24 [16547017] Mol Genet Genomics. 2006 Apr;275(4):409-20 [16485133] Trends Biochem Sci. 2006 May;31(5):247-50 [16584885] Microbiology. 2006 Aug;152(Pt 8):2221-31 [16849789] Infect Immun. 1999 Nov;67(11):6026-33 [10531263] Infect Immun. 2001 Aug;69(8):4874-83 [11447163] Cell. 2001 Sep 7;106(5):585-94 [11551506] Nat Immunol. 2002 Nov;3(11):1026-32 [12407411] Infect Immun. 2002 Dec;70(12):6741-50 [12438349] Science. 2003 Apr 25;300(5619):650-3 [12714747] Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5455-60 [12704232] Curr Opin Microbiol. 2003 Apr;6(2):135-9 [12732302] Infect Immun. 2004 Feb;72(2):844-52 [14742528] Trends Biochem Sci. 2004 May;29(5):243-9 [15130560] J Bacteriol. 2004 Jun;186(11):3304-12 [15150215] J Eukaryot Microbiol. 2004 May-Jun;51(3):364-73 [15218707] Expert Rev Vaccines. 2004 Oct;3(5):577-84 [15485337] J Bacteriol. 2004 Nov;186(21):7344-52 [15489446] J Bacteriol. 1972 Apr;110(1):368-77 [5018025] Infect Immun. 1981 May;32(2):840-51 [7251150] J Bacteriol. 1981 Sep;147(3):1063-76 [7275931] Infect Immun. 1985 May;48(2):359-65 [3988339] Infect Immun. 1987 May;55(5):1144-50 [3570458] EMBO J. 1989 Nov;8(11):3517-21 [2573517] Infect Immun. 1991 Sep;59(9):3243-53 [1715326] J Bacteriol. 1992 Mar;174(5):1454-61 [1537791] Mol Microbiol. 1994 Feb;11(3):537-44 [8152377] Infect Immun. 1996 Mar;64(3):796-809 [8641784] J Bacteriol. 1996 Aug;178(16):5049-52 [8759877] Mol Microbiol. 1996 Oct;22(1):9-19 [8899704] J Biol Chem. 1997 May 9;272(19):12265-71 [9139668] FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7 [10474183] Clin Microbiol Rev. 1999 Oct;12(4):518-53 [10515901] Infect Immun. 2005 Mar;73(3):1561-7 [15731054] J Bacteriol. 2005 Jul;187(13):4362-71 [15968045] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Emerging infectious diseases that threaten the blood supply. AN - 68384501; 17198845 AB - Following the devastating effects of blood-transmitted human immunodeficiency virus (HIV), blood establishments have become increasingly vigilant for the emergence or re-emergence of new threats to the safety of the blood supply. Many agents have fulfilled the broad definition of emerging blood-transmitted infections, including West Nile virus (WNV), Trypanosoma cruzi, Plasmodium spp., Babesia spp., parvovirus B19, dengue virus, and the prions that cause variant Creutzfeld-Jacob disease (vCJD). Other agents such as human herpes virus-8 (HHV-8-Kaposi's sarcoma virus) and Borellia (Lyme disease) and, perhaps, avian flu virus, are known to have a viremic phase, but have not yet been proved to be transfusion-transmitted. In the wake of these threats, transfusion services use a variety of donor screening interventions, including serologic assays, nucleic acid assays, and geographic exclusions based on potential exposure. The ultimate safeguard may be a pre-emptive pathogen inactivation strategy that will disrupt all nucleic acid-containing agents (though not prions). Considerable effort and resources have been invested in this arena, but currently no single technique is effective for inactivation of both liquid and cellular blood products and toxicity issues have not been completely resolved. The blood supply is remarkably safe with the risk of major pathogens such as hepatitis C virus (HCV) and HIV now reduced to less than one transmission per 2 to 3 million exposures. However, to approach near-zero infectious disease risk for emerging and re-emerging pathogens, new strategies such as pathogen inactivation or multi-pathogen microarray technology will need to be developed or refined. JF - Seminars in hematology AU - Alter, Harvey J AU - Stramer, Susan L AU - Dodd, Roger Y AD - Infectious Diseases Section and Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD 20892, USA. halter@dtm.cc.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 32 EP - 41 VL - 44 IS - 1 SN - 0037-1963, 0037-1963 KW - Index Medicus KW - Virus Diseases -- blood KW - Risk KW - Virus Diseases -- transmission KW - Parasitic Diseases -- blood KW - Humans KW - Prion Diseases -- transmission KW - Prion Diseases -- blood KW - Parasitic Diseases -- transmission KW - Communicable Diseases, Emerging -- blood KW - Blood Transfusion -- adverse effects KW - Blood Donors -- supply & distribution KW - Communicable Diseases, Emerging -- virology KW - Communicable Diseases, Emerging -- parasitology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68384501?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Seminars+in+hematology&rft.atitle=Emerging+infectious+diseases+that+threaten+the+blood+supply.&rft.au=Alter%2C+Harvey+J%3BStramer%2C+Susan+L%3BDodd%2C+Roger+Y&rft.aulast=Alter&rft.aufirst=Harvey&rft.date=2007-01-01&rft.volume=44&rft.issue=1&rft.spage=32&rft.isbn=&rft.btitle=&rft.title=Seminars+in+hematology&rft.issn=00371963&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-13 N1 - Date created - 2007-01-02 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Gene expression profiling of familial and sporadic interstitial pneumonia. AN - 68384277; 16998095 AB - Idiopathic interstitial pneumonia (IIP) and its familial variants are progressive and largely untreatable disorders with poorly understood molecular mechanisms. Both the genetics and the histologic type of IIP play a role in the etiology and pathogenesis of interstitial lung disease, but transcriptional signatures of these subtypes are unknown. To evaluate gene expression in the lung tissue of patients with usual interstitial pneumonia or nonspecific interstitial pneumonia that was either familial or nonfamilial in origin, and to compare it with gene expression in normal lung parenchyma. We profiled RNA from the lungs of 16 patients with sporadic IIP, 10 with familial IIP, and 9 normal control subjects on a whole human genome oligonucleotide microarray. Significant transcriptional differences exist in familial and sporadic IIPs. The genes distinguishing the genetic subtypes belong to the same functional categories as transcripts that distinguish IIP from normal samples. Relevant categories include chemokines and growth factors and their receptors, complement components, genes associated with cell proliferation and death, and genes in the Wnt pathway. The role of the chemokine CXCL12 in disease pathogenesis was confirmed in the murine bleomycin model of lung injury, with C57BL/6(CXCR4+/-) mice demonstrating significantly less collagen deposition than C57BL/6(CXCR4+/+) mice. Whereas substantial differences exist between familial and sporadic IIPs, we identified only minor gene expression changes between usual interstitial pneumonia and nonspecific interstitial pneumonia. Taken together, our findings indicate that differences in gene expression profiles between familial and sporadic IIPs may provide clues to the etiology and pathogenesis of IIP. JF - American journal of respiratory and critical care medicine AU - Yang, Ivana V AU - Burch, Lauranell H AU - Steele, Mark P AU - Savov, Jordan D AU - Hollingsworth, John W AU - McElvania-Tekippe, Erin AU - Berman, Katherine G AU - Speer, Marcy C AU - Sporn, Thomas A AU - Brown, Kevin K AU - Schwarz, Marvin I AU - Schwartz, David A AD - Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, P.O. Box 12233, MD B3-08, Research Triangle Park, NC 27909, USA. yangi@niehs.nih.gov Y1 - 2007/01/01/ PY - 2007 DA - 2007 Jan 01 SP - 45 EP - 54 VL - 175 IS - 1 SN - 1073-449X, 1073-449X KW - CXCL12 protein, human KW - 0 KW - CXCR4 protein, mouse KW - Chemokine CXCL12 KW - Chemokines, CXC KW - Cxcl12 protein, mouse KW - RNA, Messenger KW - Receptors, CXCR4 KW - Wnt Proteins KW - Bleomycin KW - 11056-06-7 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Chemokines, CXC -- analysis KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Lung -- chemistry KW - Gene Expression KW - Aged KW - Bleomycin -- toxicity KW - Disease Models, Animal KW - Mice KW - Lung -- pathology KW - Pulmonary Fibrosis -- genetics KW - Mice, Knockout KW - Wnt Proteins -- genetics KW - Gene Expression Profiling KW - Pulmonary Fibrosis -- chemically induced KW - Adult KW - Middle Aged KW - Receptors, CXCR4 -- genetics KW - Chemokines, CXC -- genetics KW - Male KW - Female KW - RNA, Messenger -- metabolism KW - Lung Diseases, Interstitial -- metabolism KW - RNA, Messenger -- analysis KW - Lung Diseases, Interstitial -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68384277?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+respiratory+and+critical+care+medicine&rft.atitle=Gene+expression+profiling+of+familial+and+sporadic+interstitial+pneumonia.&rft.au=Yang%2C+Ivana+V%3BBurch%2C+Lauranell+H%3BSteele%2C+Mark+P%3BSavov%2C+Jordan+D%3BHollingsworth%2C+John+W%3BMcElvania-Tekippe%2C+Erin%3BBerman%2C+Katherine+G%3BSpeer%2C+Marcy+C%3BSporn%2C+Thomas+A%3BBrown%2C+Kevin+K%3BSchwarz%2C+Marvin+I%3BSchwartz%2C+David+A&rft.aulast=Yang&rft.aufirst=Ivana&rft.date=2007-01-01&rft.volume=175&rft.issue=1&rft.spage=45&rft.isbn=&rft.btitle=&rft.title=American+journal+of+respiratory+and+critical+care+medicine&rft.issn=1073449X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-28 N1 - Date created - 2006-12-20 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6292-7 [11983918] Am J Respir Crit Care Med. 2002 May 1;165(9):1322-8 [11991887] Cell Biol Int. 2002;26(5):463-76 [12095232] Am J Respir Cell Mol Biol. 2002 Dec;27(6):705-13 [12444030] Chest. 2002 Dec;122(6 Suppl):334S-339S [12475811] Am J Pathol. 2003 May;162(5):1495-502 [12707032] Am J Respir Cell Mol Biol. 2003 Sep;29(3 Suppl):S32-6 [14503551] J Clin Invest. 2004 Jan;113(2):243-52 [14722616] Am J Respir Crit Care Med. 2000 Dec;162(6):2213-7 [11112140] Am J Respir Crit Care Med. 2001 Nov 1;164(9):1722-7 [11719316] Am J Respir Crit Care Med. 2002 Jan 15;165(2):277-304 [11790668] J Clin Invest. 2004 Jul;114(2):291-9 [15254596] J Clin Invest. 2004 Aug;114(3):438-46 [15286810] Am J Respir Cell Mol Biol. 2004 Oct;31(4):395-404 [15205180] Dis Chest. 1969 Jan;55(1):7-12 [5763753] Chest. 1970 Nov;58(5):538-40 [5507952] Am Rev Respir Dis. 1973 Aug;108(2):193-204 [4198347] Am J Med. 1977 Sep;63(3):475-80 [900150] Ann N Y Acad Sci. 1979;330:295-311 [294180] Ann N Y Acad Sci. 1979;330:333-9 [294185] Chest. 1980 Oct;78(4):591-4 [7191366] N Engl J Med. 1981 Dec 31;305(27):1617-27 [6796886] Medicine (Baltimore). 1985 May;64(3):192-202 [3921802] Clin Exp Immunol. 1985 Oct;62(1):57-64 [4064377] N Engl J Med. 1986 May 22;314(21):1343-7 [3702942] Am Rev Respir Dis. 1987 Feb;135(2):448-55 [2433976] Am J Respir Cell Mol Biol. 1994 Nov;11(5):531-9 [7946383] Exp Lung Res. 1998 Nov-Dec;24(6):721-43 [9839161] Am J Respir Crit Care Med. 1999 Sep;160(3):899-905 [10471616] Am J Pathol. 1954 Mar-Apr;30(2):263-85 [13138710] Thorax. 1964 Nov;19:515-25 [14238389] Am J Med. 1965 Sep;39:411-21 [14338292] Nat Rev Immunol. 2005 Jan;5(1):21-30 [15630426] Chest. 2005 Jan;127(1):275-83 [15653995] Am J Respir Crit Care Med. 2005 Feb 1;171(3):261-8 [15502109] J Med Genet. 2005 Jun;42(6):464-73 [15937080] Am J Respir Cell Mol Biol. 2005 Jul;33(1):9-13 [15964990] PLoS Med. 2005 Sep;2(9):e251 [16128620] Am J Respir Crit Care Med. 2005 Nov 1;172(9):1146-52 [16109978] Am J Pathol. 2005 Dec;167(6):1485-96 [16314464] Am J Respir Crit Care Med. 2006 Jan 15;173(2):188-98 [16166619] J Clin Pathol. 2006 Jan;59(1):28-39 [16394278] Comment In: Am J Respir Crit Care Med. 2008 Mar 1;177(5):558-9 [18296474] Am J Respir Crit Care Med. 2007 Jan 1;175(1):5-6 [17179494] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. AN - 68382967; 17136498 AB - Apo2L/TRAIL is actively investigated as a novel targeted agent to directly induce apoptosis of susceptible cancer cells. Apo2L/TRAIL-refractory cells can be sensitized to the cytotoxic effect of this ligand by cytotoxic chemotherapeutics. The aim of this study was to evaluate the in vitro tumoricidal activity of the Apo2L/TRAIL + Trichostatin A in cultured thoracic cancer cells and to elucidate the molecular basis of the synergistic cytotoxicity of this combination. Concurrent exposure of cultured cancer cells to sublethal concentrations of Apo2L/TRAIL and Trichostatin A resulted in profound enhancement of Apo2L/TRAIL-mediated cytotoxicity in all cell lines regardless of their intrinsic susceptibility to this ligand. This combination was not toxic to primary normal cells. While Apo2L/TRAIL alone or Trichostatin A alone mediated < 20% cell death, 60 to 90% of cancer cells were apoptotic following treatment with TSA + Apo2L/TRAIL combinations. Complete translocation of Bax from the cytosol to the mitochondria compartment was mainly observed in combination-treated cells and this was correlated with robust elevation of caspase 9 proteolytic activity indicative of activation of the mitochondria apoptogenic effect. Profound TSA + Apo2L/TRAIL-mediated cytotoxicity and apoptosis were completely abrogated by either Bcl2 over-expression or by the selective caspase 9 inhibitor, highlighting the essential role of mitochondria-dependent apoptosis signaling cascade in this process. Moreover, increased caspase 8 activity observed in cells treated with the TSA + Apo2L/TRAIL combination was completely suppressed by Bcl-2 over-expression or by the selective caspase 9 inhibitor indicating that the elevated caspase 8 activity in combination-treated cells was secondary to a mitochondria-mediated amplification feedback loop of caspase activation. These finding form the basis for further development of HDAC inhibitors + Apo2L/TRAIL combination as novel targeted therapy for thoracic malignancies. JF - Apoptosis : an international journal on programmed cell death AU - Reddy, Rishindra M AU - Yeow, Wen-Shuz AU - Chua, Alex AU - Nguyen, Duc M AU - Baras, Aris AU - Ziauddin, M Firdos AU - Shamimi-Noori, Susan M AU - Maxhimer, Justin B AU - Schrump, David S AU - Nguyen, Dao M AD - Section of Thoracic Oncology, Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 55 EP - 71 VL - 12 IS - 1 SN - 1360-8185, 1360-8185 KW - Enzyme Inhibitors KW - 0 KW - Histone Deacetylase Inhibitors KW - Hydroxamic Acids KW - Recombinant Proteins KW - TNF-Related Apoptosis-Inducing Ligand KW - TNFSF10 protein, human KW - trichostatin A KW - 3X2S926L3Z KW - Caspases KW - EC 3.4.22.- KW - Index Medicus KW - Enzyme Inhibitors -- administration & dosage KW - Enzyme Activation KW - Humans KW - Mitochondria -- drug effects KW - Mitochondria -- metabolism KW - Cell Line, Tumor KW - Drug Synergism KW - Recombinant Proteins -- administration & dosage KW - Caspases -- metabolism KW - Thoracic Neoplasms -- pathology KW - Hydroxamic Acids -- administration & dosage KW - Thoracic Neoplasms -- drug therapy KW - Apoptosis -- physiology KW - Thoracic Neoplasms -- metabolism KW - TNF-Related Apoptosis-Inducing Ligand -- administration & dosage KW - Apoptosis -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68382967?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Apoptosis+%3A+an+international+journal+on+programmed+cell+death&rft.atitle=Rapid+and+profound+potentiation+of+Apo2L%2FTRAIL-mediated+cytotoxicity+and+apoptosis+in+thoracic+cancer+cells+by+the+histone+deacetylase+inhibitor+Trichostatin+A%3A+the+essential+role+of+the+mitochondria-mediated+caspase+activation+cascade.&rft.au=Reddy%2C+Rishindra+M%3BYeow%2C+Wen-Shuz%3BChua%2C+Alex%3BNguyen%2C+Duc+M%3BBaras%2C+Aris%3BZiauddin%2C+M+Firdos%3BShamimi-Noori%2C+Susan+M%3BMaxhimer%2C+Justin+B%3BSchrump%2C+David+S%3BNguyen%2C+Dao+M&rft.aulast=Reddy&rft.aufirst=Rishindra&rft.date=2007-01-01&rft.volume=12&rft.issue=1&rft.spage=55&rft.isbn=&rft.btitle=&rft.title=Apoptosis+%3A+an+international+journal+on+programmed+cell+death&rft.issn=13608185&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-24 N1 - Date created - 2006-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Tramadol in the treatment of neuropathic cancer pain: a double-blind, placebo-controlled study. AN - 68381949; 17177582 AB - To assess the efficacy, safety and impact on quality of life of tramadol in the treatment of neuropathic pain in patients with cancer. Patients with similar characteristics were grouped in pairs and randomised to receive either tramadol or placebo. The initial tramadol dosage was 1 mg/kg every 6 hours, increasing to 1.5 mg/kg every 6 hours if necessary to control pain. The study enrolled 36 patients (22 women, 14 men), with a mean age of 50 years. In the group receiving tramadol (n = 18), major improvements in pain intensity and Karnofsky scores occurred (p < 0.001), sleep quality improved by day 45 (p < 0.05) activities of daily living improved (p < 0.05), and use of analgesics that had been taken before the study was reduced (p < 0.05) compared with the placebo group. There was no difference between the groups with regard to changes in the Zung Depression Scale, Beck Anxiety Inventory scores and neurophysiological assessments. More patients in the tramadol group experienced adverse events (p < 0.05). The most common adverse events were nausea, vomiting and constipation. Tramadol is a therapeutic option for the control of neuropathic pain in patients with cancer, and appears to improve quality of life in these patients. The analgesic effect of tramadol is independent of changes in anxiety, depression and nervous system function. JF - Clinical drug investigation AU - Arbaiza, Daniel AU - Vidal, Oscar AD - Service of Neuro-Oncology, National Cancer Institute, Lima, Peru. darbaiza@inen.sld.pe Y1 - 2007 PY - 2007 DA - 2007 SP - 75 EP - 83 VL - 27 IS - 1 SN - 1173-2563, 1173-2563 KW - Analgesics, Opioid KW - 0 KW - Tramadol KW - 39J1LGJ30J KW - Index Medicus KW - Administration, Oral KW - Double-Blind Method KW - Humans KW - Vomiting -- chemically induced KW - Patient Dropouts -- statistics & numerical data KW - Quality of Life KW - Activities of Daily Living KW - Constipation -- chemically induced KW - Appetite -- drug effects KW - Nausea -- chemically induced KW - Prospective Studies KW - Sleep -- drug effects KW - Adult KW - Treatment Outcome KW - Analgesics, Opioid -- therapeutic use KW - Middle Aged KW - Analgesics, Opioid -- administration & dosage KW - Analgesics, Opioid -- adverse effects KW - Male KW - Female KW - Karnofsky Performance Status -- statistics & numerical data KW - Neuralgia -- drug therapy KW - Neuralgia -- etiology KW - Neoplasms -- diagnosis KW - Neoplasms -- complications KW - Tramadol -- adverse effects KW - Tramadol -- therapeutic use KW - Tramadol -- administration & dosage KW - Neuralgia -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68381949?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+drug+investigation&rft.atitle=Tramadol+in+the+treatment+of+neuropathic+cancer+pain%3A+a+double-blind%2C+placebo-controlled+study.&rft.au=Arbaiza%2C+Daniel%3BVidal%2C+Oscar&rft.aulast=Arbaiza&rft.aufirst=Daniel&rft.date=2007-01-01&rft.volume=27&rft.issue=1&rft.spage=75&rft.isbn=&rft.btitle=&rft.title=Clinical+drug+investigation&rft.issn=11732563&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-07-17 N1 - Date created - 2006-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The 2006 Bernard B. Brodie Award Lecture. Cyp2e1. AN - 68381912; 17020953 AB - Bernard B. Brodie's laboratory was the first to examine the mechanisms of drug-induced toxicity at the molecular level. They found that acetaminophen hepatotoxicity was due to the metabolic activation of the drug to a highly reactive toxic metabolite that depleted cellular glutathione and covalently bound to protein. Subsequent studies revealed that activation of acetaminophen to an active metabolite is primarily carried out by CYP2E1, an ethanol-inducible cytochrome P450 that was first suggested by characterization of the microsomal ethanol oxidation system. CYP2E1 is developmentally regulated, under liver-specific control, and undergoes substrate-induced protein stabilization. It is also regulated by starvation and diabetes through insulin-dependent mRNA stabilization. In addition to acetaminophen, CYP2E1 metabolically activates a large number of low M(r) toxicants and carcinogens and thus is of great toxicological importance. The mechanism of regulation CYP2E1 and its role in acetaminophen toxicity will be discussed. JF - Drug metabolism and disposition: the biological fate of chemicals AU - Gonzalez, Frank J AD - Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37/Room 3106, Bethesda, MD 20892, USA. fjgonz@helix.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 1 EP - 8 VL - 35 IS - 1 SN - 0090-9556, 0090-9556 KW - Analgesics, Non-Narcotic KW - 0 KW - RNA, Messenger KW - Acetaminophen KW - 362O9ITL9D KW - Ethanol KW - 3K9958V90M KW - Cytochrome P-450 CYP2E1 KW - EC 1.14.13.- KW - Index Medicus KW - Animals KW - Neoplasms -- enzymology KW - Chemical and Drug Induced Liver Injury KW - Humans KW - Acetaminophen -- adverse effects KW - Ethanol -- metabolism KW - Analgesics, Non-Narcotic -- metabolism KW - Liver Diseases, Alcoholic -- enzymology KW - Analgesics, Non-Narcotic -- adverse effects KW - Ethanol -- adverse effects KW - RNA, Messenger -- metabolism KW - Microsomes -- metabolism KW - Acetaminophen -- metabolism KW - Liver Diseases -- enzymology KW - Cytochrome P-450 CYP2E1 -- metabolism KW - Cytochrome P-450 CYP2E1 -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68381912?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.atitle=The+2006+Bernard+B.+Brodie+Award+Lecture.+Cyp2e1.&rft.au=Gonzalez%2C+Frank+J&rft.aulast=Gonzalez&rft.aufirst=Frank&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Drug+metabolism+and+disposition%3A+the+biological+fate+of+chemicals&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-13 N1 - Date created - 2006-12-28 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Ethnicity-related polymorphisms and haplotypes in the human ABCB1 gene. AN - 68379317; 17187507 AB - The human multidrug resistance gene ATP-binding cassette B1 (ABCB1) codes for P-glycoprotein (P-gp), an important membrane-bound efflux transporter known to confer anticancer drug resistance as well as affect the pharmacokinetics of many drugs and xenobiotics. A number of single nucleotide polymorphisms (SNPs) have been identified throughout the ABCB1 gene that may have an effect on P-gp expression levels and function. Haplotype as well as genotype analysis of SNPs is becoming increasingly important in identifying genetic variants underlying susceptibility to human disease. Three SNPs, 1236C-->T, 2677G-->T and 3435C-->T, have been repeatedly shown to predict changes in the function of P-gp. The frequencies with which these polymorphisms exist in a population have also been shown to be ethnically related. In this study, 95 individuals representative of the entire ethnic make-up of the USA were compared with 101 individuals from an Ashkenazi-Jewish population. These individuals were analyzed by genomic sequencing and polymerase chain reaction, using restriction fragment length polymorphisms, to calculate their genotype frequencies. A total of 25 SNPs were located in the exons of the ABCB1 gene. All of the polymorphisms identified were in parts of the ABCB1 gene product predicted to be intracellular, and 16 appear to be novel as compared with those listed by the National Center for Biotechnological Information. Frequencies of the 1236C-->T and 2677G-->T/A/C SNPs were similar for the US and Ashkenazi populations (64.2 and 60.4%, respectively for 1236C-->T [chi2: 0.30; p T/A/C [chi2: 1.49; p T (24.2% for the US population and 69.3% for the Ashkenazi population [chi2: 39.927; p < or = 0.001]). The 1236T/ 2677T/3435T haplotype occurred in 23.6% (standard error: 0.013) of the Ashkenazi population. The SNP at location 3435C-->T plays a significant role in the ABCB1 gene. The haplotype and genotype analysis from these data may be used as a basis for studies on the relationship between ABCB1 genotypes and drug efficacy, drug toxicity, disease susceptibility or other phenotypes. JF - Pharmacogenomics AU - Kimchi-Sarfaty, Chava AU - Marple, Andrew H AU - Shinar, Shiri AU - Kimchi, Avraham M AU - Scavo, David AU - Roma, M Isabella AU - Kim, In-Wha AU - Jones, Adam AU - Arora, Mili AU - Gribar, John AU - Gurwitz, David AU - Gottesman, Michael M AD - National Institutes of Health, Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892-4254, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 29 EP - 39 VL - 8 IS - 1 KW - ABCB1 protein, human KW - 0 KW - P-Glycoprotein KW - P-Glycoproteins KW - Index Medicus KW - Phenotype KW - Genotype KW - Polymerase Chain Reaction KW - Genes, MDR -- genetics KW - Humans KW - Jews -- genetics KW - Genetic Predisposition to Disease KW - P-Glycoprotein -- physiology KW - Haplotypes -- genetics KW - P-Glycoprotein -- genetics KW - Polymorphism, Single Nucleotide -- genetics KW - Ethnic Groups -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68379317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Pharmacogenomics&rft.atitle=Ethnicity-related+polymorphisms+and+haplotypes+in+the+human+ABCB1+gene.&rft.au=Kimchi-Sarfaty%2C+Chava%3BMarple%2C+Andrew+H%3BShinar%2C+Shiri%3BKimchi%2C+Avraham+M%3BScavo%2C+David%3BRoma%2C+M+Isabella%3BKim%2C+In-Wha%3BJones%2C+Adam%3BArora%2C+Mili%3BGribar%2C+John%3BGurwitz%2C+David%3BGottesman%2C+Michael+M&rft.aulast=Kimchi-Sarfaty&rft.aufirst=Chava&rft.date=2007-01-01&rft.volume=8&rft.issue=1&rft.spage=29&rft.isbn=&rft.btitle=&rft.title=Pharmacogenomics&rft.issn=1744-8042&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-12-03 N1 - Date created - 2006-12-25 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Pharmacogenetics. 2004 Jun;14(6):381-5 [15247630] Am J Hum Genet. 2004 Aug;75(2):282-93 [15208782] Genes Immun. 2004 Nov;5(7):530-9 [15505619] Science. 1994 Oct 7;266(5182):107-9 [7524148] Br J Pharmacol. 1996 Nov;119(5):1038-44 [8922756] Biochemistry. 1998 Mar 17;37(11):3594-601 [9530286] Arch Intern Med. 1998 Apr 13;158(7):777-81 [9554684] Science. 2003 Oct 24;302(5645):643-6 [14576434] Pharmacogenetics. 2003 Nov;13(11):675-82 [14583680] Bull Exp Biol Med. 2003 Aug;136(2):183-5 [14631505] Am J Hum Genet. 2003 Dec;73(6):1250-60 [14624392] Am J Hum Genet. 2004 Apr;74(4):623-36 [15024686] Eur J Haematol. 2004 May;72(5):314-21 [15059065] Eur J Hum Genet. 2004 May;12(5):355-64 [14722586] Swiss Med Wkly. 2006 Jun 10;136(23-24):377-82 [16847760] Drug Metab Pharmacokinet. 2006 Jun;21(3):194-200 [16858122] Science. 2007 Jan 26;315(5811):525-8 [17185560] Nature. 1998 May 7;393(6680):79-82 [9590693] N Engl J Med. 2004 Nov 4;351(19):1972-7 [15525722] Ther Drug Monit. 2004 Dec;26(6):679-84 [15570194] Antivir Ther. 2004 Dec;9(6):929-35 [15651752] Br J Clin Pharmacol. 2005 Mar;59(3):365-70 [15752383] Arch Neurol. 2005 Mar;62(3):460-4 [15767512] Epilepsia. 2005 May;46(5):643-7 [15857428] Eur J Clin Pharmacol. 2005 Jul;61(5-6):389-94 [15912392] Biochim Biophys Acta. 2006 Jan;1762(1):17-28 [16297602] Am J Hum Genet. 2006 Mar;78(3):487-97 [16404693] Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3473-8 [10716719] Science. 2001 Feb 16;291(5507):1304-51 [11181995] Br J Pharmacol. 2001 Mar;132(6):1183-92 [11250868] Am J Hum Genet. 2001 Apr;68(4):978-89 [11254454] Pharmacogenetics. 2001 Apr;11(3):217-21 [11337937] J Pharmacol Exp Ther. 2001 Jun;297(3):1137-43 [11356939] Blood. 2001 Jun 1;97(11):3605-11 [11369657] Int J Clin Pharmacol Ther. 2001 Mar;39(3):93-101 [11396754] Clin Pharmacol Ther. 2001 Aug;70(2):189-99 [11503014] Eur J Hum Genet. 2001 Aug;9(8):634-7 [11528510] Annu Rev Med. 2002;53:615-27 [11818492] J Hum Genet. 2002;47(1):38-50 [11829140] Annu Rev Biochem. 2002;71:537-92 [12045106] J Am Soc Nephrol. 2002 Jul;13(7):1847-54 [12089380] Pharmacogenetics. 2002 Aug;12(6):437-50 [12172212] Clin Pharmacol Ther. 2002 Aug;72(2):209-19 [12189368] Transplantation. 2002 Aug 27;74(4):571-2 [12352921] Pharmacogenetics. 2002 Oct;12(7):529-34 [12360103] Biol Pharm Bull. 2002 Oct;25(10):1356-9 [12392094] Pharm Res. 2002 Oct;19(10):1581-5 [12425480] Clin Pharmacol Ther. 2002 Nov;72(5):584-94 [12426522] Annu Rev Pharmacol Toxicol. 2003;43:285-307 [12359865] Oncology. 2003;64(2):183-5 [12566917] N Engl J Med. 2003 Feb 6;348(6):538-49 [12571262] Pharmacogenomics. 2003 Mar;4(2):171-8 [12605551] Pharmacogenetics. 2003 Aug;13(8):481-94 [12893986] Am J Hum Genet. 2003 Nov;73(5):1162-9 [14574645] Oncogene. 2003 Oct 20;22(47):7468-85 [14576852] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Heteromeric nicotinic acetylcholine-dopamine autoreceptor complexes modulate striatal dopamine release. AN - 68377368; 16710311 AB - In the striatum, dopamine and acetylcholine (ACh) modulate dopamine release by acting, respectively, on dopamine D(2) autoreceptors and nicotinic ACh (nACh) heteroreceptors localized on dopaminergic nerve terminals. The possibility that functional interactions exist between striatal D(2) autoreceptors and nACh receptors was studied with in vivo microdialysis in freely moving rats. Local perfusion of nicotine in the ventral striatum (shell of the nucleus accumbens) produced a marked increase in the extracellular levels of dopamine, which was completely counteracted by co-perfusion with either the non-alpha(7) nACh receptor antagonist dihydro-beta-erythroidine or the D(2-3) receptor agonist quinpirole. Local perfusion of the D(2-3) receptor antagonist raclopride produced an increase in the extracellular levels of dopamine, which was partially, but significantly, counteracted by coperfusion with dihydro-beta-erythroidine. These findings demonstrate a potent crosstalk between G protein-coupled receptors and ligand-gated ion channels in dopaminergic nerve terminals, with the D(2) autoreceptor modulating the efficacy of non-alpha(7) nACh receptor-mediated modulation of dopamine release. We further demonstrate physical interactions between beta(2) subunits of non-alpha(7) nicotinic acetylcholine receptors and D(2) autoreceptors in co-immunoprecipitation experiments with membrane preparations from co-transfected mammalian cells and rat striatum. These results reveal that striatal non-alpha(7) nicotinic acetylcholine receptors form part of heteromeric dopamine autoreceptor complexes that modulate dopamine release. JF - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology AU - Quarta, Davide AU - Ciruela, Francisco AU - Patkar, Kshitij AU - Borycz, Janusz AU - Solinas, Marcello AU - Lluis, Carme AU - Franco, Rafael AU - Wise, Roy A AU - Goldberg, Steven R AU - Hope, Bruce T AU - Woods, Amina S AU - Ferré, Sergi AD - Behavioral Neuroscience Branch, National Institute on Drug Abuse, IRP, NIH, DHHS, Baltimore, MD 21224, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 35 EP - 42 VL - 32 IS - 1 SN - 0893-133X, 0893-133X KW - Dopamine Agonists KW - 0 KW - Dopamine Antagonists KW - Nicotinic Agonists KW - Receptors, Dopamine D2 KW - Receptors, Nicotinic KW - Quinpirole KW - 20OP60125T KW - Dihydro-beta-Erythroidine KW - 23255-54-1 KW - Raclopride KW - 430K3SOZ7G KW - Nicotine KW - 6M3C89ZY6R KW - Dopamine KW - VTD58H1Z2X KW - Index Medicus KW - Gene Expression -- drug effects KW - Animals KW - Drug Interactions KW - Dihydro-beta-Erythroidine -- pharmacology KW - Dose-Response Relationship, Drug KW - Dopamine Antagonists -- pharmacology KW - Humans KW - Raclopride -- pharmacology KW - Blotting, Western -- methods KW - Microdialysis -- methods KW - Rats KW - Transfection -- methods KW - Rats, Sprague-Dawley KW - Quinpirole -- pharmacology KW - Dopamine Agonists -- pharmacology KW - Nicotine -- pharmacology KW - Immunoprecipitation -- methods KW - Nicotinic Agonists -- pharmacology KW - Cell Line KW - Male KW - Corpus Striatum -- cytology KW - Presynaptic Terminals -- drug effects KW - Corpus Striatum -- metabolism KW - Dopamine -- metabolism KW - Corpus Striatum -- drug effects KW - Receptors, Nicotinic -- physiology KW - Receptors, Dopamine D2 -- physiology KW - Presynaptic Terminals -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68377368?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.atitle=Heteromeric+nicotinic+acetylcholine-dopamine+autoreceptor+complexes+modulate+striatal+dopamine+release.&rft.au=Quarta%2C+Davide%3BCiruela%2C+Francisco%3BPatkar%2C+Kshitij%3BBorycz%2C+Janusz%3BSolinas%2C+Marcello%3BLluis%2C+Carme%3BFranco%2C+Rafael%3BWise%2C+Roy+A%3BGoldberg%2C+Steven+R%3BHope%2C+Bruce+T%3BWoods%2C+Amina+S%3BFerr%C3%A9%2C+Sergi&rft.aulast=Quarta&rft.aufirst=Davide&rft.date=2007-01-01&rft.volume=32&rft.issue=1&rft.spage=35&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-09 N1 - Date created - 2006-12-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Silica-directed mast cell activation is enhanced by scavenger receptors. AN - 68376873; 16902192 AB - Inhalation of crystalline silica results in pulmonary fibrosis and silicosis. It has been suggested that mast cells play a role in these conditions. How mast cells would influence pathology is unknown. We thus explored mast cell interactions with silica in vitro and in B6.Cg-kit(W-sh) mast cell-deficient mice. B6.Cg-kit(W-sh) mice did not develop inflammation or significant collagen deposition after instillation of silica, while C57Bl/6 wild-type mice did have these findings. Given this supporting evidence of a role for mast cells in the development of silicosis, we examined the ability of silica to activate mouse bone marrow-derived mast cells (BMMC), including degranulation (beta-hexosaminidase release); production of reactive oxygen species (ROS) and inflammatory mediators; and the effects of silica on Fc epsilon RI-dependent activation. Silica did not induce mast cell degranulation. However, TNF-alpha, IL-13, monocyte chemotactic protein-1, protease activity, and production of ROS were dose-dependently increased after silica exposure, and production was enhanced after Fc epsilon RI stimulation. This mast cell activation was inhibited by anti-inflammatory compounds. As silica mediates some effects in macrophages through scavenger receptors (SRs), we first determined that mast cells express scavenger receptors; then explored the involvement of SR-A and macrophage receptor with colleagenous structure (MARCO). Silica-induced ROS formation, apoptosis, and TNF-alpha production were reduced in BMMC obtained from SR-A, MARCO, and SR-A/MARCO knockout mice. These findings demonstrate that silica directs mast cell production of inflammatory mediators, in part through SRs, providing insight into critical events in the pathogenesis and potential therapeutic targets in silicosis. JF - American journal of respiratory cell and molecular biology AU - Brown, Jared M AU - Swindle, Emily J AU - Kushnir-Sukhov, Nataliya M AU - Holian, Andrij AU - Metcalfe, Dean D AD - Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1881, USA. jmbrown@niaid.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 43 EP - 52 VL - 36 IS - 1 SN - 1044-1549, 1044-1549 KW - Ccl2 protein, mouse KW - 0 KW - Chemokine CCL2 KW - Interleukin-13 KW - Marco protein, mouse KW - Reactive Oxygen Species KW - Receptors, IgE KW - Receptors, Immunologic KW - Scavenger Receptors, Class A KW - Tumor Necrosis Factor-alpha KW - Silicon Dioxide KW - 7631-86-9 KW - Collagen KW - 9007-34-5 KW - Index Medicus KW - Animals KW - Reactive Oxygen Species -- metabolism KW - Cell Degranulation KW - Apoptosis KW - Collagen -- metabolism KW - Interleukin-13 -- metabolism KW - Mice KW - Chemokine CCL2 -- metabolism KW - Mice, Knockout KW - Receptors, Immunologic -- genetics KW - Receptors, IgE -- metabolism KW - Cells, Cultured KW - Receptors, Immunologic -- metabolism KW - Mice, Inbred C57BL KW - Tumor Necrosis Factor-alpha -- metabolism KW - Bone Marrow Cells -- metabolism KW - Scavenger Receptors, Class A -- genetics KW - Silicosis -- pathology KW - Mast Cells -- metabolism KW - Silicon Dioxide -- toxicity KW - Mast Cells -- physiology KW - Bone Marrow Cells -- physiology KW - Scavenger Receptors, Class A -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68376873?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+journal+of+respiratory+cell+and+molecular+biology&rft.atitle=Silica-directed+mast+cell+activation+is+enhanced+by+scavenger+receptors.&rft.au=Brown%2C+Jared+M%3BSwindle%2C+Emily+J%3BKushnir-Sukhov%2C+Nataliya+M%3BHolian%2C+Andrij%3BMetcalfe%2C+Dean+D&rft.aulast=Brown&rft.aufirst=Jared&rft.date=2007-01-01&rft.volume=36&rft.issue=1&rft.spage=43&rft.isbn=&rft.btitle=&rft.title=American+journal+of+respiratory+cell+and+molecular+biology&rft.issn=10441549&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-12 N1 - Date created - 2006-12-14 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Exp Med. 2001 Sep 17;194(6):809-21 [11560996] J Immunol. 2002 Mar 15;168(6):2953-62 [11884467] Am J Respir Cell Mol Biol. 2002 Jul;27(1):78-84 [12091249] Toxicol Sci. 2003 Mar;72(1):150-7 [12604844] Clin Exp Immunol. 2003 Mar;131(3):415-21 [12605693] Annu Rev Immunol. 2003;21:425-56 [12615888] Trends Immunol. 2003 Apr;24(4):158-61 [12697439] Free Radic Biol Med. 2003 Jun 15;34(12):1507-16 [12788471] Microcirculation. 2003 Jun;10(3-4):273-88 [12851645] Toxicol Sci. 2003 Nov;76(1):91-101 [12857937] Eur J Pharmacol. 2003 Oct 8;478(2-3):179-85 [14575803] Toxicol Sci. 2003 Nov;76(1):1-2 [14619916] Toxicology. 2004 Feb 15;195(2-3):167-76 [14751672] J Immunol. 2004 Apr 1;172(7):4068-76 [15034018] Toxicol Sci. 2004 Jul;80(1):34-48 [15056807] J Exp Med. 2004 Jul 19;200(2):267-72 [15263032] J Immunol Methods. 1988 Nov 25;115(1):61-9 [3192948] Nature. 1990 Mar 15;344(6263):245-7 [2156165] Am J Respir Cell Mol Biol. 1993 Nov;9(5):475-83 [8217187] Cytometry. 1995 May 1;20(1):23-32 [7600897] J Immunol. 1995 Jul 1;155(1):367-76 [7541421] Toxicol Appl Pharmacol. 1996 Nov;141(1):84-92 [8917679] Am J Respir Crit Care Med. 1997 Feb;155(2):761-8 [9032226] Toxicol Appl Pharmacol. 2000 Jan 15;162(2):100-6 [10637133] Am J Respir Crit Care Med. 2000 Jun;161(6):2026-34 [10852784] Pneumonol Alergol Pol. 2000;68(3-4):109-19 [11004845] Arterioscler Thromb Vasc Biol. 2000 Dec;20(12):2593-9 [11116058] Immunol Rev. 2001 Feb;179:16-24 [11292019] Toxicol Appl Pharmacol. 2001 Jul 1;174(1):10-6 [11437644] J Exp Med. 2001 Jul 16;194(2):155-64 [11457890] Nature. 1997 Mar 20;386(6622):292-6 [9069289] J Clin Invest. 1997 Mar 15;99(6):1313-21 [9077541] Clin Exp Allergy. 1998 Dec;28(12):1509-17 [10024222] J Exp Med. 1999 May 3;189(9):1497-506 [10224290] Toxicol Appl Pharmacol. 1999 Aug 1;158(3):211-20 [10438654] J Leukoc Biol. 2004 Nov;76(5):926-32 [15292275] Anal Biochem. 1965 Apr;11:1-5 [14328641] Immunol Res. 2004;30(3):339-49 [15531774] J Pathol. 2004 Dec;204(5):594-604 [15538737] Int Arch Allergy Immunol. 2004 Dec;135(4):348-56 [15564778] Am J Physiol Lung Cell Mol Physiol. 2005 Mar;288(3):L488-96 [15557088] Curr Opin Pulm Med. 2005 Mar;11(2):169-73 [15699791] Am J Physiol Lung Cell Mol Physiol. 2005 May;288(5):L841-8 [15608148] MMWR Morb Mortal Wkly Rep. 2005 Apr 29;54(16):401-5 [15858459] Exp Lung Res. 2005 Jul-Aug;31(6):581-97 [16019989] Immunology. 2005 Sep;116(1):21-9 [16108814] Am J Pathol. 2005 Sep;167(3):835-48 [16127161] Am J Physiol Lung Cell Mol Physiol. 2005 Dec;289(6):L990-8 [16040631] J Biol Chem. 2005 Dec 2;280(48):40261-70 [16176929] Environ Health Perspect. 2003 May;111(5):708-13 [12727598] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Persistent sexual arousal syndrome: a case report and review of the literature. AN - 68376209; 17162489 AB - This article describes a case of persistent sexual arousal syndrome (PSAS) seen by the Gynecology Consult Service of the National Institutes of Health. This syndrome was first described in 2001 and is characterized by excessive and unrelenting sexual arousal in the absence of desire. PSAS has only recently come to the attention of the health care community, and its prevalence in the population is not completely known. It is important to report cases of this syndrome in order to help clarify its prevalence, etiology, and prognosis and to determine possible methods of treatment. JF - Journal of sex & marital therapy AU - Mahoney, Sheila AU - Zarate, Carlos AD - National Institutes of Health, National Institute of Child Health and Human Development, Reproductive Biology and Medicine Branch, Bethesda, Maryland 0892-1109, USA. manoneys@mail.nih.gov PY - 2007 SP - 65 EP - 71 VL - 33 IS - 1 SN - 0092-623X, 0092-623X KW - Antidepressive Agents, Second-Generation KW - 0 KW - Cyclohexanols KW - Venlafaxine Hydrochloride KW - 7D7RX5A8MO KW - Index Medicus KW - Orgasm -- drug effects KW - Syndrome KW - Humans KW - Health Status KW - Bipolar Disorder -- drug therapy KW - Adult KW - Depression -- drug therapy KW - Male KW - Female KW - Antidepressive Agents, Second-Generation -- adverse effects KW - Sexual Dysfunction, Physiological -- chemically induced KW - Sexual Dysfunction, Physiological -- diagnosis KW - Cyclohexanols -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68376209?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+sex+%26+marital+therapy&rft.atitle=Persistent+sexual+arousal+syndrome%3A+a+case+report+and+review+of+the+literature.&rft.au=Mahoney%2C+Sheila%3BZarate%2C+Carlos&rft.aulast=Mahoney&rft.aufirst=Sheila&rft.date=2007-01-01&rft.volume=33&rft.issue=1&rft.spage=65&rft.isbn=&rft.btitle=&rft.title=Journal+of+sex+%26+marital+therapy&rft.issn=0092623X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-11 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Structural basis for inhibition of translation by the tumor suppressor Pdcd4. AN - 68375990; 17060447 AB - The tumor suppressor function of Programmed Cell Death 4 (Pdcd4) is achieved through interactions between Pdcd4 and components of the translation initiation complex, namely, the RNA helicase eIF4A and the scaffolding protein eIF4G. These interactions are mediated through two MA3 domains on the Pdcd4 molecule and result in inhibition of protein synthesis. We have solved the high-resolution crystal structure of the C-terminal MA3 (cMA3) domain of Pdcd4 in several crystal forms and demonstrated its similarity to the MA3 domain of eIF4G. As predicted by the structure, the cMA3 domain competes with eIF4Gc for binding to eIF4A and surprisingly is sufficient to inhibit translation initiation. Mutations that abolish eIF4A binding negate both functions of the cMA3. Interestingly mutations in the Akt phosphorylation site influenced neither cMA3 binding to eIF4A nor its ability to inhibit translation initiation. Finally, our structural analysis reveals MA3 domains to be a novel subfamily of VHS domains. JF - Molecular and cellular biology AU - LaRonde-LeBlanc, Nicole AU - Santhanam, Arti N AU - Baker, Alyson R AU - Wlodawer, Alexander AU - Colburn, Nancy H AD - Macromolecular Crystallography Laboratory, CCR, National Cancer Institute, Frederick, MD 21702, USA. nlaronde@umd.edu Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 147 EP - 156 VL - 27 IS - 1 SN - 0270-7306, 0270-7306 KW - Apoptosis Regulatory Proteins KW - 0 KW - Eukaryotic Initiation Factor-4G KW - Pdcd4 protein, mouse KW - RNA-Binding Proteins KW - Eukaryotic Initiation Factor-4A KW - EC 2.7.7.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Eukaryotic Initiation Factor-4G -- physiology KW - Phosphorylation KW - Models, Molecular KW - Two-Hybrid System Techniques KW - Molecular Sequence Data KW - Amino Acid Sequence KW - Sequence Homology, Amino Acid KW - Protein Structure, Tertiary KW - Eukaryotic Initiation Factor-4A -- physiology KW - Protein Binding KW - Protein Conformation KW - Protein Biosynthesis KW - Apoptosis Regulatory Proteins -- chemistry KW - RNA-Binding Proteins -- physiology KW - Apoptosis Regulatory Proteins -- physiology KW - Gene Expression Regulation KW - RNA-Binding Proteins -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68375990?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+and+cellular+biology&rft.atitle=Structural+basis+for+inhibition+of+translation+by+the+tumor+suppressor+Pdcd4.&rft.au=LaRonde-LeBlanc%2C+Nicole%3BSanthanam%2C+Arti+N%3BBaker%2C+Alyson+R%3BWlodawer%2C+Alexander%3BColburn%2C+Nancy+H&rft.aulast=LaRonde-LeBlanc&rft.aufirst=Nicole&rft.date=2007-01-01&rft.volume=27&rft.issue=1&rft.spage=147&rft.isbn=&rft.btitle=&rft.title=Molecular+and+cellular+biology&rft.issn=02707306&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-18 N1 - Date created - 2006-12-14 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 2ION; PDB; 2NSZ; 2IOL; 2IOS N1 - SuppNotes - Cited By: Mol Cell. 2001 Jan;7(1):193-203 [11172724] Oncogene. 2001 Feb 8;20(6):669-76 [11314000] J Biol Chem. 2001 Jan 26;276(4):2872-9 [11060291] Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41 [11517324] Mol Cell. 2001 Aug;8(2):383-96 [11545740] Int J Cancer. 2002 Mar 10;98(2):181-5 [11857405] FEBS Lett. 2002 Feb 20;513(1):19-23 [11911875] Biochem Biophys Res Commun. 2002 Sep 13;297(1):78-82 [12220511] Mol Cell Biol. 2003 Jan;23(1):26-37 [12482958] Methods Enzymol. 2003;374:22-37 [14696367] Mol Cancer Ther. 2004 Feb;3(2):103-10 [14985450] Nat Struct Mol Biol. 2004 Apr;11(4):330-7 [15004547] Mol Cell Biol. 2004 May;24(9):3894-906 [15082783] J Mol Biol. 1993 Sep 5;233(1):123-38 [8377180] Gene. 1995 Dec 12;166(2):297-301 [8543179] J Struct Biol. 1999 Apr-May;125(2-3):156-65 [10222271] Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32 [15572765] J Biol Chem. 2005 Jan 21;280(3):1872-81 [15528191] RNA. 2005 Mar;11(3):261-74 [15661843] Cancer Res. 2005 Jul 15;65(14):6034-41 [16024603] Genes Dev. 2005 Sep 15;19(18):2212-23 [16166382] Cancer Res. 2005 Dec 15;65(24):11282-6 [16357133] Mol Cell Biol. 2006 Feb;26(4):1297-306 [16449643] Structure. 2006 May;14(5):913-23 [16698552] Science. 2006 Oct 20;314(5798):467-71 [17053147] J Biomol NMR. 2006;36 Suppl 1:18 [16518566] Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14037-42 [10570194] Annu Rev Cell Dev Biol. 1999;15:269-90 [10611963] Mol Cell Biol. 2000 Jan;20(2):468-77 [10611225] Anticancer Res. 2000 May-Jun;20(3A):1343-51 [10928042] Biochemistry. 2000 Sep 19;39(37):11282-90 [10985773] Trends Biochem Sci. 2000 Sep;25(9):423-6 [10973054] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The influence of genetic polymorphisms in Ahr, CYP1A1, CYP1A2, CYP1B1, GST M1, GST T1 and UGT1A1 on urine 1-hydroxypyrene glucuronide concentrations in healthy subjects from Rio Grande do Sul, Brazil. AN - 68375286; 16864595 AB - Polymorphisms in genes encoding polycyclic aromatic hydrocarbon (PAH) metabolizing enzymes may alter metabolism of these carcinogens and contribute to inter-individual difference in urine concentrations. We investigated the influence of genetic polymorphism on PAH metabolism in urine from 199 healthy subjects from Southern Brazil. We measured urine 1-hydroxypyrene glucuronide (1-OHPG) concentrations using immunoaffinity chromatography and synchronous fluorescence spectroscopy and genotyped subjects using standard methods. Genetic variants in CYP1B1 (rs1056827, rs1800440, rs10012) were strongly associated with urine 1-OHPG with P-values < 0.010. Variants in aryl hydrocarbon receptor (Ahr) (rs4986826), CYP1A1 (rs1799814) and CYP1A2 (rs2069514) were also, although less strongly, associated with changes in urine 1-OHPG concentrations. These variants had P-values of 0.074, 0.040 and 0.025, respectively. The median urine 1-OHPG concentrations (pmol/ml) in the homozygous wild-type and homozygous variants for CYP1B1 (rs10012) and the Ahr, CYP1A1 and CYP1A2 variants listed above were 2.16 and 0.10, 2.16 and 0.41, 2.03 and 0.46, 2.19 and 2.79, respectively. We found no effect of deletions in GST M1 or GST T1, or different alleles of UGT1A1*28. Adjusting for age, sex, place of residence, tobacco smoke exposure, maté drinking, cachaça and barbeque preparation had only a minor impact on the associations. A model containing just exposure variables had an r2 of 0.21; a model with single genotypes for Ahr, CYP1A1, CYP1A2 and CYP1B1 had an r2 of 0.10; and a model combining both exposure and genotype information had a total r2 of 0.33. Our results suggest that CYP1B1 genotypes are strongly associated with urine 1-OHPG concentrations in this population. JF - Carcinogenesis AU - Abnet, Christian C AU - Fagundes, Renato B AU - Strickland, Paul T AU - Kamangar, Farin AU - Roth, Mark J AU - Taylor, Philip R AU - Dawsey, Sanford M AD - Nutritional Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Boulevard, EPS/320, MSC 7232, Rockville, MD 20852, USA. abnetc@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 112 EP - 117 VL - 28 IS - 1 SN - 0143-3334, 0143-3334 KW - 1-hydroxypyrene-glucuronide KW - 0 KW - Glucuronates KW - Pyrenes KW - Receptors, Aryl Hydrocarbon KW - Aryl Hydrocarbon Hydroxylases KW - EC 1.14.14.1 KW - CYP1B1 protein, human KW - Cytochrome P-450 CYP1A1 KW - Cytochrome P-450 CYP1A2 KW - Cytochrome P-450 CYP1B1 KW - UGT1A1 enzyme KW - EC 2.4.1.- KW - Glucuronosyltransferase KW - EC 2.4.1.17 KW - glutathione S-transferase T1 KW - EC 2.5.1.- KW - Glutathione Transferase KW - EC 2.5.1.18 KW - glutathione S-transferase M1 KW - Index Medicus KW - Chromatography, Affinity KW - Humans KW - Brazil -- epidemiology KW - Middle Aged KW - Male KW - Female KW - Glucuronosyltransferase -- genetics KW - Cytochrome P-450 CYP1A1 -- genetics KW - Cytochrome P-450 CYP1A2 -- genetics KW - Polymorphism, Genetic KW - Glucuronates -- urine KW - Receptors, Aryl Hydrocarbon -- genetics KW - Glutathione Transferase -- genetics KW - Aryl Hydrocarbon Hydroxylases -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68375286?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=The+influence+of+genetic+polymorphisms+in+Ahr%2C+CYP1A1%2C+CYP1A2%2C+CYP1B1%2C+GST+M1%2C+GST+T1+and+UGT1A1+on+urine+1-hydroxypyrene+glucuronide+concentrations+in+healthy+subjects+from+Rio+Grande+do+Sul%2C+Brazil.&rft.au=Abnet%2C+Christian+C%3BFagundes%2C+Renato+B%3BStrickland%2C+Paul+T%3BKamangar%2C+Farin%3BRoth%2C+Mark+J%3BTaylor%2C+Philip+R%3BDawsey%2C+Sanford+M&rft.aulast=Abnet&rft.aufirst=Christian&rft.date=2007-01-01&rft.volume=28&rft.issue=1&rft.spage=112&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-08 N1 - Date created - 2006-12-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetic impairment of frontocortical endocannabinoid degradation and high alcohol preference. AN - 68372236; 16482090 AB - Endocannabinoid signaling has recently been implicated in ethanol-seeking behavior. We analyzed the expression of endocannabinoid-related genes in key brain regions of reward and dependence, and compared them between the alcohol-preferring AA (Alko Alcohol) and nonpreferring ANA (Alko Non-Alcohol) rat lines. A decreased expression of fatty acid amidohydrolase (FAAH), the main endocannabinoid-degrading enzyme, was found in prefrontal cortex (PFC) of AA rats, and was accompanied by decreased enzyme activity in this region. Binding of the endocannabinoid-cannabinoid 1 (CB1) receptor ligand (3)[H]SR141716A, and [35S]GTPgammaS incorporation stimulated by the CB1 agonist WIN 55,212-2 were downregulated in the same area. Together, this suggests an overactive endocannabinoid transmission in the PFC of AA animals, and a compensatory downregulation of CB1 signaling. The functional role of impaired FAAH function for alcohol self-administration was validated in two independent ways. The CB1 antagonist SR141716A potently and dose-dependently suppressed self-administration in AA rats when given systemically, or locally into the PFC, but not in the striatum. Conversely, intra-PFC injections of the competitive FAAH inhibitor URB597 increased ethanol self-administration in nonselected Wistar rats. These results show for the first time that impaired FAAH function may confer a phenotype of high voluntary alcohol intake, and point to a FAAH both as a potential susceptibility factor and a therapeutic target. JF - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology AU - Hansson, Anita C AU - Bermúdez-Silva, Francisco J AU - Malinen, Hanna AU - Hyytiä, Petri AU - Sanchez-Vera, Irene AU - Rimondini, Roberto AU - Rodriguez de Fonseca, Fernando AU - Kunos, George AU - Sommer, Wolfgang H AU - Heilig, Markus AD - Laboratory of Clinical and Translational Studies, NIAAA, National Institutes of Health, Bethesda, MD 20892-1108, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 117 EP - 126 VL - 32 IS - 1 SN - 0893-133X, 0893-133X KW - Analgesics KW - 0 KW - Benzamides KW - Benzoxazines KW - Cannabinoid Receptor Modulators KW - Carbamates KW - Central Nervous System Depressants KW - Endocannabinoids KW - Morpholines KW - Naphthalenes KW - Piperidines KW - Pyrazoles KW - RNA, Messenger KW - Receptor, Cannabinoid, CB1 KW - cyclohexyl carbamic acid 3'-carbamoylbiphenyl-3-yl ester KW - Guanosine 5'-O-(3-Thiotriphosphate) KW - 37589-80-3 KW - Ethanol KW - 3K9958V90M KW - Win 55212-2 KW - 5H31GI9502 KW - Amidohydrolases KW - EC 3.5.- KW - fatty-acid amide hydrolase KW - EC 3.5.1.- KW - rimonabant KW - RML78EN3XE KW - Index Medicus KW - Naphthalenes -- pharmacology KW - Carbamates -- pharmacology KW - Animals KW - Drug Interactions KW - Dose-Response Relationship, Drug KW - Brain Chemistry -- drug effects KW - Piperidines -- pharmacokinetics KW - Morpholines -- pharmacology KW - Ethanol -- administration & dosage KW - Analgesics -- pharmacology KW - Reverse Transcriptase Polymerase Chain Reaction -- methods KW - Pyrazoles -- pharmacokinetics KW - Central Nervous System Depressants -- administration & dosage KW - Rats KW - Amidohydrolases -- metabolism KW - Behavior, Animal -- drug effects KW - Self Administration -- methods KW - RNA, Messenger -- metabolism KW - Benzamides -- pharmacology KW - Amidohydrolases -- genetics KW - Brain Chemistry -- genetics KW - In Situ Hybridization -- methods KW - Guanosine 5'-O-(3-Thiotriphosphate) -- pharmacokinetics KW - Male KW - Gene Expression -- drug effects KW - Cannabinoid Receptor Modulators -- metabolism KW - Prefrontal Cortex -- metabolism KW - Receptor, Cannabinoid, CB1 -- genetics KW - Receptor, Cannabinoid, CB1 -- metabolism KW - Alcohol Drinking -- genetics KW - Gene Expression -- physiology KW - Prefrontal Cortex -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68372236?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.atitle=Genetic+impairment+of+frontocortical+endocannabinoid+degradation+and+high+alcohol+preference.&rft.au=Hansson%2C+Anita+C%3BBerm%C3%BAdez-Silva%2C+Francisco+J%3BMalinen%2C+Hanna%3BHyyti%C3%A4%2C+Petri%3BSanchez-Vera%2C+Irene%3BRimondini%2C+Roberto%3BRodriguez+de+Fonseca%2C+Fernando%3BKunos%2C+George%3BSommer%2C+Wolfgang+H%3BHeilig%2C+Markus&rft.aulast=Hansson&rft.aufirst=Anita&rft.date=2007-01-01&rft.volume=32&rft.issue=1&rft.spage=117&rft.isbn=&rft.btitle=&rft.title=Neuropsychopharmacology+%3A+official+publication+of+the+American+College+of+Neuropsychopharmacology&rft.issn=0893133X&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-09 N1 - Date created - 2006-12-14 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Immunohistochemical analysis of expressions of hepatic cytochrome P450 in F344 rats following oral treatment with kava extract. AN - 68369025; 17059882 AB - Kava (Piper methysticum), used for relaxation and pain relief, has been one of the leading dietary supplements and several reports linking hepatic functional disturbances and liver failure to kava have resulted in a ban on sales in Europe and Canada and the issuance of warnings by the US FDA. The National Toxicology Program conducted 14-week rat studies to characterize the toxicology of kava exposure in Fischer 344 rats [National Toxicity Program. 90 day gavage toxicity studies of KAVA KAVA EXTRACT in Fischer rats and B6C3F1 mice. Research Triangle Park, NC; 2005a; National Toxicity Program. Testing status of agents at NTP (KAVA KAVA EXTRACT M990058). Research Triangle Park, NC; 2005b. (http://ntp.niehs.nih.gov/index.cfm?objectid=071516E-C6E1-7AAA-C90C751E23D14C1B)]. Groups of 10 male and 10 female rats were administered kava extract by gavage at 0, 0.125, 0.25, 0.5, 1.0, and 2.0 g/kg/day. Increased gamma-glutamyl-transpeptidase (GGT) activities were observed in the 2.0 g/kg males and 1.0 and 2.0 g/kg females, as well as increased serum cholesterol levels in males and females at 0.5 g/kg and higher. Increases in incidence and severity of hepatocellular hypertrophy (HP) were noted in males at 1.0 g/kg and females at 0.5 g/kg and higher, as well as increased liver weights. Immunohistochemical analyses of the expression of cytochrome-P450 (CYP) enzymes in liver of the control and 1.0- and 2.0-g/kg-treated groups indicated decreased expression of CYP2D1 (human CYP2D6 homolog) in 2.0 g/kg females and increased expression of CYP1A2, 2B1, and 3A1 in 1.0 and 2.0 g/kg groups of both sexes. The no observed adverse effect levels were decided as 0.25 g/kg in both genders, based on neurotoxic effects, increases in GGT, cholesterol, liver weight, and HP and decreases in body weight. Kava-induced hepatic functional changes in the F344 rat might be relevant to human clinical cases of hepatotoxicity following exposure. JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie AU - Clayton, Natasha P AU - Yoshizawa, Katsuhiko AU - Kissling, Grace E AU - Burka, Leo T AU - Chan, Po-Chuen AU - Nyska, Abraham AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 223 EP - 236 VL - 58 IS - 4 SN - 0940-2993, 0940-2993 KW - Blood Glucose KW - 0 KW - Cytochrome P-450 Enzyme System KW - 9035-51-2 KW - Cholesterol KW - 97C5T2UQ7J KW - gamma-Glutamyltransferase KW - EC 2.3.2.2 KW - Index Medicus KW - Administration, Oral KW - Animals KW - Dose-Response Relationship, Drug KW - Blood Glucose -- drug effects KW - Dietary Supplements -- toxicity KW - Liver Function Tests KW - Rats KW - Cholesterol -- blood KW - Rats, Inbred F344 KW - gamma-Glutamyltransferase -- drug effects KW - gamma-Glutamyltransferase -- blood KW - Hypertrophy -- chemically induced KW - Immunohistochemistry KW - Female KW - Male KW - Liver -- pathology KW - Liver -- enzymology KW - Liver -- drug effects KW - Cytochrome P-450 Enzyme System -- metabolism KW - Kava -- toxicity KW - Cytochrome P-450 Enzyme System -- drug effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68369025?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.atitle=Immunohistochemical+analysis+of+expressions+of+hepatic+cytochrome+P450+in+F344+rats+following+oral+treatment+with+kava+extract.&rft.au=Clayton%2C+Natasha+P%3BYoshizawa%2C+Katsuhiko%3BKissling%2C+Grace+E%3BBurka%2C+Leo+T%3BChan%2C+Po-Chuen%3BNyska%2C+Abraham&rft.aulast=Clayton&rft.aufirst=Natasha&rft.date=2007-01-01&rft.volume=58&rft.issue=4&rft.spage=223&rft.isbn=&rft.btitle=&rft.title=Experimental+and+toxicologic+pathology+%3A+official+journal+of+the+Gesellschaft+fur+Toxikologische+Pathologie&rft.issn=09402993&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-22 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Cell Mol Med. 2002 Apr-Jun;6(2):189-98 [12169204] J Am Acad Child Adolesc Psychiatry. 2002 Jun;41(6):631-2 [12049436] Drug Metab Dispos. 2002 Nov;30(11):1153-7 [12386118] Phytomedicine. 2003;10(5):440-6 [12834011] J Toxicol Clin Toxicol. 2003;41(2):109-13 [12733846] Planta Med. 2002 Dec;68(12):1055-8 [12494328] Planta Med. 2003 Jun;69(6):496-9 [12865965] Phytochemistry. 2003 Oct;64(3):673-9 [13679089] J Toxicol Clin Toxicol. 2003;41(6):821-9 [14677792] Naunyn Schmiedebergs Arch Pharmacol. 2004 Jan;369(1):89-104 [14574440] Toxicol Lett. 2004 Apr 15;150(1):85-96 [15068826] Planta Med. 2004 Mar;70(3):193-6 [15114493] J Ethnopharmacol. 2004 Aug;93(2-3):153-60 [15234747] Biometrics. 1971 Mar;27(1):103-17 [5547548] Lancet. 1971 Aug 14;2(7720):376-7 [4105075] Biometrics. 1972 Jun;28(2):519-31 [5037867] Biometrics. 1977 Jun;33(2):386-9 [884197] J Natl Cancer Inst. 1979 Apr;62(4):957-74 [285297] Biometrics. 1986 Mar;42(1):183-6 [3719054] Med J Aust. 1988 Jun 6;148(11):548-55 [3374423] Clin Exp Pharmacol Physiol. 1990 Jul;17(7):509-14 [2401104] J Ethnopharmacol. 1992 Aug;37(1):13-45 [1453702] Planta Med. 1994 Feb;60(1):88-90 [8134424] J Am Acad Dermatol. 1994 Jul;31(1):89-97 [8021378] Drug Metab Rev. 1998 Aug;30(3):441-98 [9710703] Food Chem Toxicol. 1998 Sep-Oct;36(9-10):831-9 [9737431] Eur J Clin Pharmacol. 1998 Jul;54(5):431-5 [9754989] Toxicol Sci. 1999 Apr;48(2):151-6 [10353305] Pharmacogenomics J. 2005;5(1):6-13 [15492763] CMAJ. 2005 Feb 1;172(3):367-79 [15684121] Exp Mol Pathol. 2005 Apr;78(2):150-5 [15713442] J Infus Nurs. 2005 Mar-Apr;28(2):108-17 [15785331] Arch Pharm Res. 2005 Mar;28(3):249-68 [15832810] Clin Pharmacol Ther. 2005 May;77(5):415-26 [15900287] Acta Pharmacol Sin. 2005 Jun;26(6):737-44 [15916741] Drugs. 2005;65(9):1239-82 [15916450] Expert Opin Drug Saf. 2005 Jul;4(4):779-94 [16011454] J Ethnopharmacol. 2005 Aug 22;100(1-2):108-13 [16005588] Geriatrics. 2005 Sep;60(9):24-5 [16153141] Drug Metab Dispos. 2005 Oct;33(10):1555-63 [16033948] Ann Pharmacother. 2005 Oct;39(10):1634-9 [16159994] J Clin Pharm Ther. 2000 Jun;25(3):197-220 [10886465] Chem Res Toxicol. 2001 Jun;14(6):611-50 [11409933] Rinsho Byori. 2001 Nov;Suppl 116:62-71 [11797381] Toxicol Pathol. 2002 Jan-Feb;30(1):88-92 [11890481] Life Sci. 2002 Apr 19;70(22):2581-97 [12269386] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development. AN - 68368378; 17055228 AB - Matrix metalloproteinases (MMPs) are a large family of extracellular or membrane-bound proteases. Their ability to cleave extracellular matrix (ECM) proteins has implicated a role in ECM remodeling to affect cell fate and behavior during development and in pathogenesis. We have shown previously that membrane-type 1 (MT1)-MMP [corrected] is coexpressed temporally and spatially with the MMP gelatinase A (GelA) in all cell types of the intestine and tail where GelA is expressed during Xenopus laevis metamorphosis, suggesting a cooperative role of these MMPs in development. Here, we show that Xenopus GelA and MT1-MMP interact with each other in vivo and that overexpression of MT1-MMP and GelA together in Xenopus embryos leads to the activation of pro-GelA. We further show that both MMPs are expressed during Xenopus embryogenesis, although MT1-MMP gene is expressed earlier than the GelA gene. To investigate whether the embryonic MMPs play a role in development, we have studied whether precocious expression of these MMPs alters development. Our results show that overexpression of both MMPs causes developmental abnormalities and embryonic death by a mechanism that requires the catalytic activity of the MMPs. More importantly, we show that coexpression of wild type MT1-MMP and GelA leads to a cooperative effect on embryonic development and that this cooperative effect is abolished when the catalytic activity of either MMP is eliminated through a point mutation in the catalytic domain. Thus, our studies support a cooperative role of these MMPs in embryonic development, likely through the activation of pro-GelA by MT1-MMP. JF - Mechanisms of development AU - Hasebe, Takashi AU - Hartman, Rebecca AU - Fu, Liezhen AU - Amano, Tosikazu AU - Shi, Yun-Bo AD - Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bldg. 18T, Rm. 106, Bethesda, MD 20892, USA. Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 11 EP - 22 VL - 124 IS - 1 SN - 0925-4773, 0925-4773 KW - Recombinant Proteins KW - 0 KW - DNA KW - 9007-49-2 KW - Matrix Metalloproteinase 2 KW - EC 3.4.24.24 KW - Matrix Metalloproteinase 14 KW - EC 3.4.24.80 KW - Index Medicus KW - Animals KW - Extracellular Matrix -- metabolism KW - Enzyme Activation KW - Animals, Genetically Modified KW - Recombinant Proteins -- genetics KW - Mutagenesis, Site-Directed KW - Base Sequence KW - Gene Expression Regulation, Enzymologic KW - Recombinant Proteins -- metabolism KW - DNA -- genetics KW - Catalytic Domain -- genetics KW - Point Mutation KW - Recombinant Proteins -- chemistry KW - Protein Structure, Tertiary KW - Gene Expression Regulation, Developmental KW - Xenopus laevis -- genetics KW - Xenopus laevis -- metabolism KW - Matrix Metalloproteinase 14 -- metabolism KW - Matrix Metalloproteinase 2 -- genetics KW - Xenopus laevis -- embryology KW - Matrix Metalloproteinase 14 -- chemistry KW - Matrix Metalloproteinase 2 -- metabolism KW - Matrix Metalloproteinase 14 -- genetics KW - Matrix Metalloproteinase 2 -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68368378?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mechanisms+of+development&rft.atitle=Evidence+for+a+cooperative+role+of+gelatinase+A+and+membrane+type-1+matrix+metalloproteinase+during+Xenopus+laevis+development.&rft.au=Hasebe%2C+Takashi%3BHartman%2C+Rebecca%3BFu%2C+Liezhen%3BAmano%2C+Tosikazu%3BShi%2C+Yun-Bo&rft.aulast=Hasebe&rft.aufirst=Takashi&rft.date=2007-01-01&rft.volume=124&rft.issue=1&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Mechanisms+of+development&rft.issn=09254773&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-27 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Dev Biol. 1998 Nov 1;203(1):24-35 [9806770] J Cell Sci. 1999 Mar;112 ( Pt 6):773-84 [10036228] APMIS. 1999 Jan;107(1):38-44 [10190278] APMIS. 1999 Jan;107(1):137-43 [10190290] Cell Res. 1999 Jun;9(2):91-105 [10418731] Cell. 1999 Oct 1;99(1):81-92 [10520996] Cell Res. 2005 Mar;15(3):150-9 [15780176] J Biol Chem. 2005 Jul 1;280(26):25079-86 [15878869] J Biol Chem. 2005 Jul 29;280(30):27856-65 [15929979] J Biol Chem. 2005 Dec 23;280(51):42237-41 [16251193] Cell Tissue Res. 2006 Apr;324(1):105-16 [16418836] Int J Mol Med. 1998 Sep;2(3):273-82 [9855698] Dev Biol. 1998 Nov 1;203(1):12-23 [9806769] J Biol Chem. 1999 Nov 26;274(48):34260-6 [10567400] Cell Res. 1999 Dec;9(4):291-303 [10628838] Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4052-7 [10737763] Genes Dev. 2000 Sep 1;14(17):2123-33 [10970876] J Cell Biol. 2000 Sep 4;150(5):1177-88 [10974004] EMBO J. 2001 Sep 3;20(17):4782-93 [11532942] Curr Opin Cell Biol. 2001 Oct;13(5):534-40 [11544020] Annu Rev Cell Dev Biol. 2001;17:463-516 [11687497] Cell Res. 2001 Dec;11(4):245-52 [11787769] Dev Dyn. 2002 Mar;223(3):402-13 [11891989] Science. 2002 Mar 29;295(5564):2387-92 [11923519] Mol Reprod Dev. 2002 Aug;62(4):470-6 [12112579] Oncogene. 2002 Aug 29;21(38):5861-7 [12185585] Mol Biotechnol. 2002 Sep;22(1):51-86 [12353914] Matrix Biol. 2003 May;22(3):279-93 [12853038] Curr Opin Cell Biol. 2004 Oct;16(5):558-64 [15363807] Biochim Biophys Acta. 1987 Nov 25;907(3):191-217 [2823896] J Biol Chem. 1988 Aug 25;263(24):11892-9 [2841336] Int Rev Cytol. 1989;119:97-149 [2695486] Proc Natl Acad Sci U S A. 1990 Jul;87(14):5578-82 [2164689] Int J Cancer. 1992 Mar 12;50(5):791-5 [1544713] Matrix Suppl. 1992;1:237-44 [1480033] Crit Rev Oral Biol Med. 1993;4(2):197-250 [8435466] Semin Cell Biol. 1993 Jun;4(3):161-73 [8347833] Curr Opin Cell Biol. 1993 Oct;5(5):891-7 [8240832] Annu Rev Cell Biol. 1993;9:541-73 [8280471] Curr Opin Cell Biol. 1993 Dec;5(6):1029-35 [8129940] Biochemistry. 1994 May 31;33(21):6684-90 [7911325] J Biol Chem. 1994 Oct 14;269(41):25328-34 [7929226] Int J Dev Biol. 1994 Jun;38(2):345-50 [7981043] Dev Biol. 1995 Jan;167(1):252-62 [7851646] Nature. 1995 May 18;375(6528):244-7 [7746327] Cell Tissue Res. 1996 Feb;283(2):325-9 [8593661] Kidney Int Suppl. 1996 May;54:S68-74 [8731199] Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1924-9 [8700860] Cancer Metastasis Rev. 1995 Dec;14(4):351-62 [8821095] Mol Biol Cell. 1996 Oct;7(10):1471-83 [8898355] Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1402-7 [9037065] Curr Opin Cell Biol. 1997 Oct;9(5):701-6 [9330874] J Cell Biol. 1998 Mar 23;140(6):1535-41 [9508784] EMBO J. 1998 Apr 15;17(8):2298-307 [9545242] Cell. 1998 May 1;93(3):411-22 [9590175] Cell Res. 1998 Sep;8(3):171-7 [9791730] Cell Res. 1998 Sep;8(3):179-86 [9791731] Cell Res. 1998 Sep;8(3):187-94 [9791732] Curr Opin Cell Biol. 1998 Oct;10(5):602-8 [9818170] Erratum In: Mech Dev. 2007 May;124(5):407-8 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mapping convulsants' binding to the GABA-A receptor chloride ionophore: a proposed model for channel binding sites. AN - 68368288; 16959376 AB - Gamma-aminobutyric acid (GABA) type A receptors play a key role in brain inhibitory neurotransmission, and are ligand-activated chloride channels blocked by numerous convulsant ligands. Here we summarize data on binding of picrotoxin, tetrazoles, beta-lactams, bicyclophosphates, butyrolactones and neurotoxic pesticides to GABA-A ionophore, and discuss functional and structural overlapping of their binding sites. The paper reviews data on convulsants' binding sensitivity to different point mutations in ionophore-lining second trans-membrane domains of GABA-A subunits, and maps possible location of convulsants' sites within the chloride ionophore. We also discuss data on inhibition of glycine, glutamate, serotonin (5-HT3) and N-acetylcholine receptors by GABA-A channel blockers, and examine the applicability of this model to other homologous ionotropic receptors. Positioning various convulsant-binding sites within ionophore of GABA-A receptors, this model enables a better understanding of complex architectonics of ionotropic receptors, and may be used for developing new channel-modulating drugs. JF - Neurochemistry international AU - Kalueff, A V AD - Laboratory of Clinical Science, Building 10, Room 3D41, National Institute of Mental Health (NIMH), NIH, 10 Center Dr. MSC 1264, Bethesda, MD 20892-1264, USA. kalueva@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 61 EP - 68 VL - 50 IS - 1 SN - 0197-0186, 0197-0186 KW - Convulsants KW - 0 KW - Ligands KW - Receptors, GABA-A KW - Index Medicus KW - Animals KW - Humans KW - Binding Sites KW - Convulsants -- metabolism KW - Receptors, GABA-A -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68368288?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neurochemistry+international&rft.atitle=Mapping+convulsants%27+binding+to+the+GABA-A+receptor+chloride+ionophore%3A+a+proposed+model+for+channel+binding+sites.&rft.au=Kalueff%2C+A+V&rft.aulast=Kalueff&rft.aufirst=A&rft.date=2007-01-01&rft.volume=50&rft.issue=1&rft.spage=61&rft.isbn=&rft.btitle=&rft.title=Neurochemistry+international&rft.issn=01970186&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-20 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Pharmacol Exp Ther. 1990 Aug;254(2):578-83 [2166797] Mol Pharmacol. 1991 Jan;39(1):79-84 [1846222] Life Sci. 1991;49(11):PL49-54 [1875786] J Physiol. 1992 Feb;447:191-213 [1317428] J Physiol. 1992 Jan;445:97-127 [1323672] Brain Res. 1992 Nov 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Jun;44(8):994-1002 [12763092] Nature. 2003 Jun 26;423(6943):949-55 [12827192] Curr Drug Targets CNS Neurol Disord. 2003 Aug;2(4):207-12 [12871031] Curr Drug Targets CNS Neurol Disord. 2003 Aug;2(4):213-32 [12871032] Neuropharmacology. 2003 Sep;45(3):304-14 [12871648] Eur J Pharmacol. 2003 Aug 22;476(1-2):17-24 [12969744] Brain Res Mol Brain Res. 2003 Nov 26;119(2):207-12 [14625088] FEBS Lett. 2003 Nov 27;555(1):91-5 [14630325] Arch Insect Biochem Physiol. 2003 Dec;54(4):145-56 [14635176] Neurotoxicology. 2003 Dec;24(6):817-24 [14637376] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - The biological bases of nicotine and alcohol co-addiction. AN - 68364998; 17161671 JF - Biological psychiatry AU - Li, Ting-Kai AU - Volkow, Nora D AU - Baler, Ruben D AU - Egli, Mark AD - National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892-9589, USA. Y1 - 2007/01/01/ PY - 2007 DA - 2007 Jan 01 SP - 1 EP - 3 VL - 61 IS - 1 SN - 0006-3223, 0006-3223 KW - Index Medicus KW - Animals KW - Humans KW - Alcohol-Related Disorders -- complications KW - Alcohol-Related Disorders -- genetics KW - Tobacco Use Disorder -- therapy KW - Tobacco Use Disorder -- epidemiology KW - Tobacco Use Disorder -- genetics KW - Alcohol-Related Disorders -- therapy KW - Tobacco Use Disorder -- complications KW - Biology KW - Alcohol-Related Disorders -- epidemiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68364998?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biological+psychiatry&rft.atitle=The+biological+bases+of+nicotine+and+alcohol+co-addiction.&rft.au=Li%2C+Ting-Kai%3BVolkow%2C+Nora+D%3BBaler%2C+Ruben+D%3BEgli%2C+Mark&rft.aulast=Li&rft.aufirst=Ting-Kai&rft.date=2007-01-01&rft.volume=61&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Biological+psychiatry&rft.issn=00063223&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-20 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Asthma phenotypes. AN - 68359974; 17133120 AB - Asthma is a heterogeneous disorder presenting with many phenotypes. Precise phenotypic definition has eluded the medical research community for years, despite recognition of different disease subtypes. Improved phenotypic characterization and knowledge of underlying pathobiology is necessary for linkage of specific genotypes with clinical disease manifestations. Phenotyping has been difficult because asthma is likely to be comprised of overlapping syndromes with varying origins and heterogeneous pathobiology. Currently, the field is too reliant on classification by trigger or symptoms. Since genotypic and phenotypic heterogeneity are inherent in asthma, patients presenting with different asthma phenotypes may need tailored therapies. Studies have begun to link genetics with disease mechanism and therapeutic response. As disease etiology, onset, progression and severity vary greatly among patients, however, the relative contribution of genetic factors may be difficult to ascertain. Definition of the full array of complex biological consequences of molecular target modulation is a prerequisite for therapies based on this concept. The advent of targeted therapies for asthma and clinical trials based on phenotype and genotype have raised interest in more accurate description of asthma phenotypes. Therapies based on phenotypic and genotypic characteristics may be useful in asthma management. A variety of factors, however, must be addressed before such approaches become standard. JF - Current opinion in pulmonary medicine AU - Kiley, James AU - Smith, Robert AU - Noel, Patricia AD - Division of Lung Diseases, National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-7952, USA. kileyj@nhlbi.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 19 EP - 23 VL - 13 IS - 1 SN - 1070-5287, 1070-5287 KW - Glucocorticoids KW - 0 KW - Aspirin KW - R16CO5Y76E KW - Index Medicus KW - Hypersensitivity -- complications KW - Genotype KW - Pulmonary Ventilation -- physiology KW - Aspirin -- adverse effects KW - Humans KW - Glucocorticoids -- adverse effects KW - Genetic Predisposition to Disease KW - Phenotype KW - Asthma -- etiology KW - Asthma -- genetics KW - Asthma -- physiopathology KW - Asthma -- pathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68359974?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+opinion+in+pulmonary+medicine&rft.atitle=Asthma+phenotypes.&rft.au=Kiley%2C+James%3BSmith%2C+Robert%3BNoel%2C+Patricia&rft.aulast=Kiley&rft.aufirst=James&rft.date=2007-01-01&rft.volume=13&rft.issue=1&rft.spage=19&rft.isbn=&rft.btitle=&rft.title=Current+opinion+in+pulmonary+medicine&rft.issn=10705287&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-15 N1 - Date created - 2006-11-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Genetics, biology and clinical management of myeloid cell primary immune deficiencies: chronic granulomatous disease and leukocyte adhesion deficiency. AN - 68357553; 17133097 AB - Chronic granulomatous disease and leukocyte adhesion deficiency are the major primary immune deficiencies affecting phagocytic blood cells. Major advances in clinical diagnosis and development of novel treatments for these disorders merit review. Clinically beneficial gene therapy correction of X-linked chronic granulomatous disease in two adult patients was reported. Nonmyeloablative busulfan conditioning before administration of gene corrected autologous hematopoietic stem cells was likely an essential maneuver to achieve successful gene therapy. There is an increased association of autoimmune disorders with chronic granulomatous disease. Preimplantation genetic diagnosis of leukocyte adhesion deficiency-I led to the birth of a normal child. A canine model of leukocyte adhesion deficiency-I facilitated development of new nonmyeloablative hematopoietic stem cell transplant and gene therapy approaches to leukocyte adhesion deficiency. Nonmyeloablative transplantation may provide an effective, but less toxic approach for leukocyte adhesion deficiency in children. There have been advances in understanding the basis of leukocyte adhesion deficiency-II and III. The most important subjects reviewed in this chapter include new advances in development of gene therapy for chronic granulomatous disease and leukocyte adhesion deficiency-I; transplantation for leukocyte adhesion deficiency-I; prenatal diagnosis of leukocyte adhesion deficiency-I; and association of autoimmune diseases with chronic granulomatous disease. JF - Current opinion in hematology AU - Malech, Harry L AU - Hickstein, Dennis D AD - Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20982-1456, USA. hmalech@nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 29 EP - 36 VL - 14 IS - 1 SN - 1065-6251, 1065-6251 KW - Index Medicus KW - Models, Animal KW - Animals KW - Humans KW - Hematopoietic Stem Cell Transplantation KW - Dogs KW - Preimplantation Diagnosis KW - Female KW - Pregnancy KW - Neutrophils -- immunology KW - Leukocyte-Adhesion Deficiency Syndrome -- genetics KW - Genetic Therapy -- methods KW - Granulomatous Disease, Chronic -- therapy KW - Granulomatous Disease, Chronic -- genetics KW - Leukocyte-Adhesion Deficiency Syndrome -- diagnosis KW - Leukocyte-Adhesion Deficiency Syndrome -- therapy KW - Granulomatous Disease, Chronic -- immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68357553?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+opinion+in+hematology&rft.atitle=Genetics%2C+biology+and+clinical+management+of+myeloid+cell+primary+immune+deficiencies%3A+chronic+granulomatous+disease+and+leukocyte+adhesion+deficiency.&rft.au=Malech%2C+Harry+L%3BHickstein%2C+Dennis+D&rft.aulast=Malech&rft.aufirst=Harry&rft.date=2007-01-01&rft.volume=14&rft.issue=1&rft.spage=29&rft.isbn=&rft.btitle=&rft.title=Current+opinion+in+hematology&rft.issn=10656251&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-23 N1 - Date created - 2006-11-29 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Experimental determination of calibration settings of a commercially available radionuclide calibrator for various clinical measurement geometries and radionuclides. AN - 68355471; 16996744 AB - Following the approach of the National Primary Laboratory of the UK (NPL) for the calibration of radionuclide calibrators, but using a commercially available instrument with no data available in the literature, the radionuclide calibrator response was investigated as a function of different measurement geometries at the "Regina Elena" National Cancer Institute (IRE) in Rome. Working with Italian National Metrology Institute for ionising radiation quantities (ENEA-INMRI), specific calibration factors with traceability to national primary standards were determined for different types of glass vials, solid capsules and plastic syringes, investigating three radionuclides with different energy spectra (Tc-99m, In-111, I-131). For each kind of syringe, calibration correction factors for different filling volumes were calculated. For Tc-99m and I-131 the difference between measured and true activity was in the range 2-7%, depending on measurement geometry. For In-111 a large percentage deviation from the true activity value was found in each geometry considered, reaching 35%. The magnitude of this difference is particularly dependent on the energies of the emitted photons. JF - Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine AU - Ceccatelli, A AU - Benassi, M AU - D'Andrea, M AU - De Felice, P AU - Fazio, A AU - Nocentini, S AU - Strigari, L AD - Laboratorio di Fisica Medica e Sistemi Esperti, Regina Elena National Cancer Institute, via E. Chianesi, 53 - 00144 Rome, Italy. alessia.ceccatelli@tiscali.it Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 120 EP - 125 VL - 65 IS - 1 SN - 0969-8043, 0969-8043 KW - Radioisotopes KW - 0 KW - Radiopharmaceuticals KW - Index Medicus KW - Sensitivity and Specificity KW - Radiation Dosage KW - Reference Values KW - European Union KW - Reproducibility of Results KW - Reference Standards KW - Calibration KW - Radiopharmaceuticals -- analysis KW - Radiometry -- instrumentation KW - Radioisotopes -- standards KW - Guidelines as Topic KW - Radiopharmaceuticals -- standards KW - Radioisotopes -- analysis KW - Radiometry -- standards UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68355471?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+radiation+and+isotopes+%3A+including+data%2C+instrumentation+and+methods+for+use+in+agriculture%2C+industry+and+medicine&rft.atitle=Experimental+determination+of+calibration+settings+of+a+commercially+available+radionuclide+calibrator+for+various+clinical+measurement+geometries+and+radionuclides.&rft.au=Ceccatelli%2C+A%3BBenassi%2C+M%3BD%27Andrea%2C+M%3BDe+Felice%2C+P%3BFazio%2C+A%3BNocentini%2C+S%3BStrigari%2C+L&rft.aulast=Ceccatelli&rft.aufirst=A&rft.date=2007-01-01&rft.volume=65&rft.issue=1&rft.spage=120&rft.isbn=&rft.btitle=&rft.title=Applied+radiation+and+isotopes+%3A+including+data%2C+instrumentation+and+methods+for+use+in+agriculture%2C+industry+and+medicine&rft.issn=09698043&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-09 N1 - Date created - 2006-11-13 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Molecular genetic structure-function analysis of translation initiation factor eIF5B. AN - 68343003; 17913624 AB - Recently, significant progress has been made in obtaining three-dimensional (3-D) structures of the factors that promote translation initiation, elongation, and termination. These structures, when interpreted in light of previous biochemical characterizations of the factors, provide significant insight into the function of the factors and the molecular mechanism of specific steps in the translation process. In addition, genetic analyses in yeast have helped elucidate the in vivo roles of the factors in various steps of the translation pathway. We have combined these two approaches and use molecular genetic studies to define the structure-function properties of translation initiation factors in the yeast Saccharomyces cerevisiae. In this chapter, we describe our multistep approach in which we first characterize a site-directed mutant of the factor of interest using in vivo and in vitro assays of protein synthesis. Next, we subject the mutant gene to random mutagenesis and screen for second-site mutations that restore the factor's function in vivo. Following biochemical and in vivo characterization of the suppressor mutant, we interpret the results in light of the 3-D structure of the factor to define the structure-function properties of the factor and to provide new molecular insights into the mechanism of translation. JF - Methods in enzymology AU - Shin, Byung-Sik AU - Dever, Thomas E AD - Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 185 EP - 201 VL - 429 SN - 0076-6879, 0076-6879 KW - Cell Extracts KW - 0 KW - Eukaryotic Initiation Factor-2 KW - Eukaryotic Initiation Factors KW - FUN12 protein, S cerevisiae KW - Saccharomyces cerevisiae Proteins KW - eukaryotic initiation factor-5B KW - GTP Phosphohydrolases KW - EC 3.6.1.- KW - Index Medicus KW - Mutagenesis, Site-Directed KW - Cell Fractionation KW - Saccharomyces cerevisiae Proteins -- genetics KW - Suppression, Genetic -- genetics KW - Polyribosomes -- physiology KW - GTP Phosphohydrolases -- analysis KW - Eukaryotic Initiation Factor-2 -- genetics KW - Ribosomes -- physiology KW - Saccharomyces cerevisiae -- genetics KW - Eukaryotic Initiation Factors -- chemistry KW - Eukaryotic Initiation Factors -- physiology KW - Eukaryotic Initiation Factors -- genetics KW - Saccharomyces cerevisiae -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68343003?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Methods+in+enzymology&rft.atitle=Molecular+genetic+structure-function+analysis+of+translation+initiation+factor+eIF5B.&rft.au=Shin%2C+Byung-Sik%3BDever%2C+Thomas+E&rft.aulast=Shin&rft.aufirst=Byung-Sik&rft.date=2007-01-01&rft.volume=429&rft.issue=&rft.spage=185&rft.isbn=&rft.btitle=&rft.title=Methods+in+enzymology&rft.issn=00766879&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-12-03 N1 - Date created - 2007-10-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Osmotic stress and DNA damage. AN - 68286132; 17875421 AB - Mammalian renal inner medullary cells are normally exposed to extremely high NaCl concentrations. The interstitial NaCl concentration in parts of a normal renal medulla can be 500 mM or more, depending on the species. Remarkably, under these normal conditions, the high NaCl causes DNA damage, yet the cells survive and function both in cell culture and in vivo. Both in cell culture and in vivo the breaks are repaired rapidly if the NaCl concentration is lowered. This chapter describes two methods used to detect and study the DNA damage induced by osmotic stress: comet assay or single cell electrophoresis and TUNEL assay or in situ labeling of 3'-OH ends of DNA strands. This chapter also discusses how specifics of the protocols influence the conclusions about types of DNA damage and what the limitations of these methods are for detecting different types of DNA damage. JF - Methods in enzymology AU - Dmitrieva, Natalia I AU - Burg, Maurice B AD - Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 241 EP - 252 VL - 428 SN - 0076-6879, 0076-6879 KW - Index Medicus KW - Comet Assay -- methods KW - In Situ Nick-End Labeling -- methods KW - DNA Damage -- physiology KW - Osmotic Pressure KW - Apoptosis -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68286132?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Methods+in+enzymology&rft.atitle=Osmotic+stress+and+DNA+damage.&rft.au=Dmitrieva%2C+Natalia+I%3BBurg%2C+Maurice+B&rft.aulast=Dmitrieva&rft.aufirst=Natalia&rft.date=2007-01-01&rft.volume=428&rft.issue=&rft.spage=241&rft.isbn=&rft.btitle=&rft.title=Methods+in+enzymology&rft.issn=00766879&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-11-05 N1 - Date created - 2007-09-18 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Intentional exposure studies of environmental agents on human subjects: assessing benefits and risks. AN - 68261529; 17847606 AB - In this article, I assess the benefits and risks of studies that intentionally expose research subjects to environmental agents. I describe these types of studies, identify their benefits and risks, compare them to other research methods that can be used to investigate the relationship between environmental exposures and disease, and discuss some issues related to research design and risk minimization. I argue that the benefits of intentional environmental exposure studies outweigh the risks when 1) the knowledge gained is likely to improve our understanding of the relationship between environmental exposure and disease, 2) this knowledge cannot be obtained by other methods, 3) the experiments are well designed, 4) the subjects will receive some benefits, such as medical evaluations, 5) risks are minimized, and 6) the risks to human subjects are less than those encountered in a typical Phase I drug study. Only in rare circumstances (i.e., when an intentional environmental exposure study is needed to implement an important environmental or public health intervention or regulation) may such studies expose research subjects to risks as high as those encountered in a typical Phase I drug trail. JF - Accountability in research AU - Resnik, David B AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. resnikd@niehs.nih.gov PY - 2007 SP - 35 EP - 55 VL - 14 IS - 1 SN - 0898-9621, 0898-9621 KW - Pesticides KW - 0 KW - Bioethics KW - Index Medicus KW - United States KW - Animals KW - Humans KW - United States Environmental Protection Agency -- ethics KW - Risk Assessment -- ethics KW - Environmental Exposure -- ethics KW - Environmental Exposure -- adverse effects KW - Ethics, Research KW - Pesticides -- adverse effects UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68261529?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Accountability+in+research&rft.atitle=Intentional+exposure+studies+of+environmental+agents+on+human+subjects%3A+assessing+benefits+and+risks.&rft.au=Resnik%2C+David+B&rft.aulast=Resnik&rft.aufirst=David&rft.date=2007-01-01&rft.volume=14&rft.issue=1&rft.spage=35&rft.isbn=&rft.btitle=&rft.title=Accountability+in+research&rft.issn=08989621&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-27 N1 - Date created - 2007-09-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: J Contemp Health Law Policy. 2000 Summer;16(2):427-58 [10921235] Environ Health Perspect. 2004 Mar;112(3):A150-1; author reply A151-2; discussion A152-6 [14998762] Environ Health Perspect. 2004 Feb;112(2):142-7 [14754567] J Natl Med Assoc. 2002 Oct;94(10 Suppl):1-26 [12401060] Environ Health Perspect. 2004 Jun;112(8):914-9 [15175182] Ann Neurol. 2006 Aug;60(2):197-203 [16802290] JAMA. 2000 May 24-31;283(20):2701-11 [10819955] Am J Bioeth. 2006 May-Jun;6(3):W1-13 [16754430] N Engl J Med. 2006 May 4;354(18):1869-71 [16672696] Environ Health Perspect. 2004 Jun;112(8):A458-9 [15175189] Environ Health Perspect. 2004 Sep;112(13):1275-81 [15345339] Am J Public Health. 2004 Nov;94(11):1908-16 [15514226] Environ Health Perspect. 1993 Oct;101(5):396-401 [8119247] Regul Toxicol Pharmacol. 1995 Feb;21(1):75-80; discussion 81-6 [7784639] Arch Toxicol. 2004 Oct;78(10):565-74 [15150681] J Nutr. 2005 Feb;135(2):283-6 [15671227] Environ Health Perspect. 2005 Jul;113(7):813-7 [16002367] Exp Toxicol Pathol. 2005 Jul;57 Suppl 1:143-6 [16092721] JAMA. 2005 Aug 17;294(7):826-32 [16106008] Environ Health Perspect. 2006 Feb;114(2):209-12 [16451856] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Roles of Smad3 in TGF-beta signaling during carcinogenesis. AN - 68214814; 17725494 AB - Signaling of transforming growth factor beta (TGF-beta) is mediated through a heteromeric complex of two types of transmembrane receptors and downstream intracellular proteins known as Smads. Alterations of TGF-beta signaling underlie various forms of human cancer and developmental diseases. Human genetic studies have revealed both point mutations and deletions of Smad2 or Smad4 in several types of cancers. However, the role of Smad3 in tumorigenesis is not clear. Recent data indicate that Smad3 also functions as a tumor suppressor by inhibiting cell proliferation and promoting apoptosis. In addition, Smad3 is essential for TGF-beta-mediated immune suppression, and it plays an important role in regulating transcriptional responses that are favorable to metastasis. Therefore, through regulating different transcriptional responses, Smad3 functions as both a negative and positive regulator of carcinogenesis depending on cell type and clinical stage of the tumor. JF - Critical reviews in eukaryotic gene expression AU - Millet, Caroline AU - Zhang, Ying E AD - Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 2056B, Bethesda, MD 20892-4256, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 281 EP - 293 VL - 17 IS - 4 SN - 1045-4403, 1045-4403 KW - SMAD3 protein, human KW - 0 KW - Smad3 Protein KW - Transforming Growth Factor beta KW - Index Medicus KW - Animals KW - Liver Neoplasms -- pathology KW - Humans KW - Neoplasm Metastasis KW - Carcinoma, Hepatocellular -- pathology KW - Mice KW - Liver Neoplasms -- physiopathology KW - Carcinoma, Hepatocellular -- physiopathology KW - Mutation KW - Signal Transduction -- physiology KW - Smad3 Protein -- physiology KW - Transforming Growth Factor beta -- metabolism KW - Cell Transformation, Neoplastic KW - Smad3 Protein -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68214814?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Critical+reviews+in+eukaryotic+gene+expression&rft.atitle=Roles+of+Smad3+in+TGF-beta+signaling+during+carcinogenesis.&rft.au=Millet%2C+Caroline%3BZhang%2C+Ying+E&rft.aulast=Millet&rft.aufirst=Caroline&rft.date=2007-01-01&rft.volume=17&rft.issue=4&rft.spage=281&rft.isbn=&rft.btitle=&rft.title=Critical+reviews+in+eukaryotic+gene+expression&rft.issn=10454403&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-10-24 N1 - Date created - 2007-08-29 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nat Cell Biol. 2004 Apr;6(4):358-65 [15048128] Cell. 2004 Apr 16;117(2):211-23 [15084259] Development. 2004 Apr;131(8):1717-28 [15084457] Nat Cell Biol. 2004 Apr;6(4):366-72 [15104092] J Cell Sci. 1999 Dec;112 ( Pt 24):4557-68 [10574705] Cell. 2000 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Biol. 2001 Nov;11(11):S44-51 [11684442] Nat Cell Biol. 2002 Jan;4(1):51-8 [11740493] Curr Opin Genet Dev. 2002 Feb;12(1):22-9 [11790550] Mol Cell. 2002 Jan;9(1):133-43 [11804592] J Cell Biol. 2002 Jan 21;156(2):299-313 [11790801] Mol Cell Biol. 2002 Mar;22(5):1369-78 [11839804] Nat Rev Immunol. 2002 Jan;2(1):46-53 [11905837] EMBO J. 2002 Jul 15;21(14):3749-59 [12110587] Nat Rev Drug Discov. 2002 Feb;1(2):111-21 [12120092] Cell. 2002 Jul 12;110(1):19-32 [12150994] Nat Rev Cancer. 2002 Aug;2(8):584-93 [12154351] Nat Rev Cancer. 2002 Jun;2(6):442-54 [12189386] Nature. 2002 Oct 17;419(6908):729-34 [12384701] EMBO J. 2002 Dec 2;21(23):6473-82 [12456654] Mol Cell Biol. 1999 Sep;19(9):5913-22 [10454538] Blood. 1999 Oct 15;94(8):2854-61 [10515889] Genes Dev. 2005 Jan 1;19(1):152-63 [15630024] Clin Cancer Res. 2005 Jan 15;11(2 Pt 2):937s-43s [15701890] Mol Biol Cell. 2005 Apr;16(4):1987-2002 [15689496] J Cell Physiol. 2005 Jul;204(1):260-72 [15690394] Nature. 2005 Jun 2;435(7042):677-81 [15902208] J Clin Invest. 2005 Jul;115(7):1714-23 [15937546] J Cell Sci. 2005 Aug 15;118(Pt 16):3573-84 [16105881] Nat Rev Cancer. 2003 Nov;3(11):807-21 [14557817] Am J Pathol. 2004 Feb;164(2):651-63 [14742269] Mol Cell Biol. 2004 Mar;24(6):2546-59 [14993291] EMBO J. 2004 Mar 10;23(5):1155-65 [14976548] J Immunol. 2004 Apr 1;172(7):4275-84 [15034041] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - New approaches for monitoring CTL activity in clinical trials. AN - 68196300; 17713015 AB - We have developed a modification of the ELISPOT assay that measures Granzyme B (GrB) release from cytotoxic T lymphocytes (CTLs). The GrB ELISPOT assay is a superior alternative to the 51Cr-release assay since it is significantly more sensitive and provides an estimation of cytotoxic effector cell frequency. Additionally, unlike the IFN-gamma ELISPOT assay, the GrB ELISPOT directly measures the release of a cytolytic protein. We report that the GrB ELISPOT can be utilized to measure ex vivo antigen-specific cytotoxicity of peripheral blood mononuclear cells (PBMCs) from cancer patients vaccinated with a peptide-based cancer vaccine. We compare the reactivity of patients' PBMCs in the GrB ELISPOT, with reactivity in the tetramer, IFN-gamma ELISPOT and chromium (51Cr)-release assays. Differences in immune response over all assays tested were found between patients, and four response patterns were observed. Reactivity in the GrB ELISPOT was more closely associated with cytotoxicity in the 51Cr-release assay than the tetramer or IFN-gamma ELISPOT assays. We also optimized the GrB ELISPOT assay to directly measure immune responses against autologous primary tumor cells in vaccinated cancer patients. A perforin ELISPOT assay was also adapted to evaluate peptide-stimulated reactivity of PMBCs from vaccinated melanoma patients. Modifications of the ELISPOT assay described in this chapter allow a more comprehensive evaluation of low-frequency tumor-specific CTLs and their specific effector functions and can provide a valuable insight into immune responses in cancer vaccine trials. JF - Advances in experimental medicine and biology AU - Malyguine, Anatoli AU - Strobl, Susan AU - Zaritskaya, Liubov AU - Baseler, Michael AU - Shafer-Weaver, Kimberly AD - Applied and Developmental Research Support Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD, USA. amalyguine@ncifcrf.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 273 EP - 284 VL - 601 SN - 0065-2598, 0065-2598 KW - Cancer Vaccines KW - 0 KW - Chromium Radioisotopes KW - Membrane Glycoproteins KW - Pore Forming Cytotoxic Proteins KW - Perforin KW - 126465-35-8 KW - Granzymes KW - EC 3.4.21.- KW - Index Medicus KW - Membrane Glycoproteins -- chemistry KW - Cytotoxicity Tests, Immunologic -- methods KW - Animals KW - Cancer Vaccines -- chemistry KW - Humans KW - Granzymes -- chemistry KW - Chromium Radioisotopes -- chemistry KW - Pore Forming Cytotoxic Proteins -- chemistry KW - Immune System KW - Enzyme-Linked Immunosorbent Assay -- methods KW - T-Lymphocytes, Cytotoxic -- cytology KW - T-Lymphocytes, Cytotoxic -- immunology KW - Clinical Trials as Topic -- methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68196300?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Advances+in+experimental+medicine+and+biology&rft.atitle=New+approaches+for+monitoring+CTL+activity+in+clinical+trials.&rft.au=Malyguine%2C+Anatoli%3BStrobl%2C+Susan%3BZaritskaya%2C+Liubov%3BBaseler%2C+Michael%3BShafer-Weaver%2C+Kimberly&rft.aulast=Malyguine&rft.aufirst=Anatoli&rft.date=2007-01-01&rft.volume=601&rft.issue=&rft.spage=273&rft.isbn=&rft.btitle=&rft.title=Advances+in+experimental+medicine+and+biology&rft.issn=00652598&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-10-03 N1 - Date created - 2007-08-23 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - An immortalized rat ventral mesencephalic cell line, RTC4, is protective in a rodent model of stroke. AN - 68182322; 17708338 AB - One therapeutic approach to stroke is the transplantation of cells capable of trophic support, reinnervation, and/or regeneration. Previously, we have described the use of novel truncated isoforms of SV40 large T antigen to generate unique cell lines from several primary rodent tissue types. Here we describe the generation of two cell lines, RTC3 and RTC4, derived from primary mesencephalic tissue using a fragment of mutant T antigen, T155c (cDNA) expressed from the RSV promoter. Both lines expressed the glial markers vimentin and S100beta, but not the neuronal markers NeuN, MAP2, or beta-III-tubulin. A screen for secreted trophic factors revealed substantially elevated levels of platelet-derived growth factor (PDGF) in RTC4, but not RTC3 cells. When transplanted into rat cortex, RTC4 cells survived for at least 22 days and expressed PDGF. Because PDGF has been reported to reduce ischemic injury, we examined the protective functions of RTC4 cells in an animal model of stroke. RTC4 or RTC3 cells, or vehicle, were injected into rat cortex 15-20 min prior to a 60-min middle cerebral artery ligation. Forty-eight hours later, animals were sacrificed and the stroke volume was assessed by triphenyl-tetrazolium chloride (TTC) staining. Compared to vehicle or RTC3 cells, transplanted RTC4 cells significantly reduced stroke volume. Overall, we generated a cell line with glial properties that produces PDGF and reduces ischemic injury in a rat model of stroke. JF - Cell transplantation AU - Harvey, B K AU - Chen, G J AU - Schoen, C J AU - Lee, C T AU - Howard, D B AU - Dillon-Carter, O AU - Coggiano, M AU - Freed, W J AU - Wang, Y AU - Hoffer, B J AU - Sanchez, J F AD - Molecular Neuropsychiatry Research Branch, National Institute on Drug Abuse, National Institutes of Health (NIH), Baltimore, MD 21224, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 483 EP - 491 VL - 16 IS - 5 SN - 0963-6897, 0963-6897 KW - Growth Substances KW - 0 KW - Platelet-Derived Growth Factor KW - Index Medicus KW - Rats KW - Phenotype KW - Animals KW - Rats, Sprague-Dawley KW - Growth Substances -- secretion KW - Cell Death KW - Cerebral Infarction -- prevention & control KW - Platelet-Derived Growth Factor -- secretion KW - Disease Models, Animal KW - Cell Line, Transformed KW - Male KW - Cerebral Infarction -- chemically induced KW - Cell Survival KW - Mesencephalon -- transplantation KW - Stroke -- prevention & control KW - Mesencephalon -- cytology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68182322?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+transplantation&rft.atitle=An+immortalized+rat+ventral+mesencephalic+cell+line%2C+RTC4%2C+is+protective+in+a+rodent+model+of+stroke.&rft.au=Harvey%2C+B+K%3BChen%2C+G+J%3BSchoen%2C+C+J%3BLee%2C+C+T%3BHoward%2C+D+B%3BDillon-Carter%2C+O%3BCoggiano%2C+M%3BFreed%2C+W+J%3BWang%2C+Y%3BHoffer%2C+B+J%3BSanchez%2C+J+F&rft.aulast=Harvey&rft.aufirst=B&rft.date=2007-01-01&rft.volume=16&rft.issue=5&rft.spage=483&rft.isbn=&rft.btitle=&rft.title=Cell+transplantation&rft.issn=09636897&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-07 N1 - Date created - 2007-08-21 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Brain Res Mol Brain Res. 2003 May 12;113(1-2):44-51 [12750005] J Neurosci. 2003 Aug 27;23(21):7958-65 [12944527] Exp Neurol. 2003 Sep;183(1):47-55 [12957487] Gene Ther. 2003 Dec;10(26):2105-11 [14625564] Cell Transplant. 2004;13(3):319-27 [15191169] Kobe J Med Sci. 2004 Jan;50(1-2):21-30 [15342968] Proc Natl Acad Sci U S A. 1978 May;75(5):2473-7 [209467] Mol Cell Biol. 1986 Apr;6(4):1172-8 [3023875] Cell. 1987 Jan 30;48(2):321-30 [3026642] J Virol. 1992 Mar;66(3):1289-93 [1310750] Exp Neurol. 1993 Dec;124(2):368-71 [8287932] Exp Neurol. 1993 Dec;124(2):401-12 [7904571] Cell Transplant. 1993 Mar-Apr;2(2):151-62 [7908247] Brain Res. 1994 Apr 18;643(1-2):162-72 [8032912] Brain Res. 2001 May 11;900(2):268-76 [11334807] J Cereb Blood Flow Metab. 1999 Dec;19(12):1329-35 [10598937] Cancer Lett. 2003 Mar 20;192(1):97-107 [12637158] Exp Neurol. 2002 Jun;175(2):318-37 [12061863] Exp Neurol. 1994 Aug;128(2):191-201 [8076662] J Virol. 1998 Mar;72(3):2224-32 [9499080] Metab Brain Dis. 1997 Dec;12(4):271-80 [9475500] Cell Tissue Res. 1998 Feb;291(2):175-89 [9426306] J Cereb Blood Flow Metab. 1997 Oct;17(10):1097-106 [9346435] J Cereb Blood Flow Metab. 1997 May;17(5):500-6 [9183287] J Neurosci. 1997 Jun 1;17(11):4341-8 [9151750] J Mol Neurosci. 2005;27(2):245-60 [16186635] J Neurosurg. 2005 Jul;103(1):38-45 [16121971] Brain Res. 1999 Sep 18;842(1):211-4 [10526112] Stroke. 1998 Jul;29(7):1417-22 [9660398] Brain Res. 1998 Feb 16;784(1-2):250-5 [9518639] Neurosci Res. 1997 Dec;29(4):335-43 [9527625] Stroke. 1997 Mar;28(3):564-73 [9056612] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Trastuzumab in the adjuvant treatment of breast cancer. AN - 68142515; 17685010 AB - Four large randomized trials to assess efficacy and toxicity of trastuzumab in adjuvant systemic therapy of breast cancer have been initiated. Results clearly demonstrate, that adjuvant treatment of trastuzumab significantly improves outcomes for women with HER2 positive breast cancer. The clinically most significant adverse events of trastuzumab are serious-infusion related reactions and cardiotoxicity. Benefit for patient should be considered according to advantage versus risk (Tab. 1, Fig. 2, Ref. 17) Full Text (Free, PDF) www.bmj.sk. JF - Bratislavske lekarske listy AU - Svetlovska, D AU - Mardiak, J AU - Kristova, V AD - National Cancer Institute, Bratislava, Slovakia. daniela.svetlovska@nou.sk Y1 - 2007 PY - 2007 DA - 2007 SP - 100 EP - 103 VL - 108 IS - 2 SN - 0006-9248, 0006-9248 KW - Antibodies, Monoclonal KW - 0 KW - Antibodies, Monoclonal, Humanized KW - Antineoplastic Agents KW - Receptor, ErbB-2 KW - EC 2.7.10.1 KW - Trastuzumab KW - P188ANX8CK KW - Index Medicus KW - Humans KW - Female KW - Chemotherapy, Adjuvant KW - Breast Neoplasms -- drug therapy KW - Antineoplastic Agents -- therapeutic use KW - Antibodies, Monoclonal -- therapeutic use UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68142515?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bratislavske+lekarske+listy&rft.atitle=Trastuzumab+in+the+adjuvant+treatment+of+breast+cancer.&rft.au=Svetlovska%2C+D%3BMardiak%2C+J%3BKristova%2C+V&rft.aulast=Svetlovska&rft.aufirst=D&rft.date=2007-01-01&rft.volume=108&rft.issue=2&rft.spage=100&rft.isbn=&rft.btitle=&rft.title=Bratislavske+lekarske+listy&rft.issn=00069248&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-09-18 N1 - Date created - 2007-08-09 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Synthesis and evaluation of ethylnitrosoureas of substituted naphthalimides as anticancer compounds. AN - 68110872; 17665847 AB - Four new ethylnitrosourea derivatives of substituted naphthalimides 3a-d have been synthesized from the respective N-(2-ethylamino) naphthalimides. Their chemical alkylating activity compared with the clinical drug CCNU and an experimental compound Mitonafide indicated that they possess lower alkylating activity than CCNU and comparable activity with the latter. Their anti-tumor efficacies were assessed in vivo in two murine ascites tumors namely Sarcoma-180 (S-180) and Ehrlich ascites carcinoma (EAC) by measuring the increase in median survival times (MST) of drug treated (T) over untreated control (C) mice. CCNU and Mitonafide were used as positive controls for comparison. The representative compound 3a has displayed marginal anti-tumoral activity in these tumors. Three compounds were further screened in vitro in 4 different human tumor cell lines but no significant activity was observed in those lines. These compounds moderately inhibit the synthesis of DNA and RNA in S-180 tumor cells. JF - Acta poloniae pharmaceutica AU - Pain, Anindita AU - Samanta, Suva AU - Dutta, Sushanta AU - Saxena, Ajit K AU - Shanmugavel, Mutiah AU - Sharma, Madhunika AU - Qazi, Gulam N AU - Sanyal, Utpal AD - Department of Anticancer Drug Development, Chittaranjan National Cancer Institute, Kolkata - 700026, India. PY - 2007 SP - 27 EP - 33 VL - 64 IS - 1 SN - 0001-6837, 0001-6837 KW - Antineoplastic Agents, Alkylating KW - 0 KW - Imides KW - Isoquinolines KW - Naphthalimides KW - Nitrosourea Compounds KW - mitonafide KW - 06Q0V17SI9 KW - RNA KW - 63231-63-0 KW - Lomustine KW - 7BRF0Z81KG KW - DNA KW - 9007-49-2 KW - Index Medicus KW - Animals KW - Drug Screening Assays, Antitumor KW - Dose-Response Relationship, Drug KW - Humans KW - Lomustine -- pharmacology KW - Cell Line, Tumor KW - Imides -- pharmacology KW - Mice KW - DNA -- biosynthesis KW - RNA -- drug effects KW - Structure-Activity Relationship KW - DNA -- drug effects KW - RNA -- biosynthesis KW - Isoquinolines -- pharmacology KW - Nitrosourea Compounds -- pharmacology KW - Nitrosourea Compounds -- chemistry KW - Antineoplastic Agents, Alkylating -- chemical synthesis KW - Antineoplastic Agents, Alkylating -- pharmacology KW - Naphthalimides -- chemistry KW - Sarcoma 180 -- drug therapy KW - Nitrosourea Compounds -- chemical synthesis KW - Carcinoma, Ehrlich Tumor -- drug therapy KW - Nitrosourea Compounds -- administration & dosage KW - Naphthalimides -- chemical synthesis KW - Antineoplastic Agents, Alkylating -- chemistry KW - Naphthalimides -- pharmacology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68110872?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Acta+poloniae+pharmaceutica&rft.atitle=Synthesis+and+evaluation+of+ethylnitrosoureas+of+substituted+naphthalimides+as+anticancer+compounds.&rft.au=Pain%2C+Anindita%3BSamanta%2C+Suva%3BDutta%2C+Sushanta%3BSaxena%2C+Ajit+K%3BShanmugavel%2C+Mutiah%3BSharma%2C+Madhunika%3BQazi%2C+Gulam+N%3BSanyal%2C+Utpal&rft.aulast=Pain&rft.aufirst=Anindita&rft.date=2007-01-01&rft.volume=64&rft.issue=1&rft.spage=27&rft.isbn=&rft.btitle=&rft.title=Acta+poloniae+pharmaceutica&rft.issn=00016837&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-11-30 N1 - Date created - 2007-08-01 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. AN - 68095240; 17472752 AB - Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging, neurodegeneration, and carcinogenesis. However, DNA repair is poorly characterized in this organism. We adapted a quantitative polymerase chain reaction assay to characterize repair of DNA damage induced by ultraviolet type C (UVC) radiation in C. elegans, and then tested whether DNA repair rates were affected by age in adults. UVC radiation induced lesions in young adult C. elegans, with a slope of 0.4 to 0.5 lesions per 10 kilobases of DNA per 100 J/m2, in both nuclear and mitochondrial targets. L1 and dauer larvae were more than fivefold more sensitive to lesion formation than were young adults. Nuclear repair kinetics in a well expressed nuclear gene were biphasic in nongravid adult nematodes: a faster, first order (half-life about 16 hours) phase lasting approximately 24 hours and resulting in removal of about 60% of the photoproducts was followed by a much slower phase. Repair in ten nuclear DNA regions was 15% and 50% higher in more actively transcribed regions in young and aging adults, respectively. Finally, repair was reduced by 30% to 50% in each of the ten nuclear regions in aging adults. However, this decrease in repair could not be explained by a reduction in expression of nucleotide excision repair genes, and we present a plausible mechanism, based on gene expression data, to account for this decrease. Repair of UVC-induced DNA damage in C. elegans is similar kinetically and genetically to repair in humans. Furthermore, this important repair process slows significantly in aging C. elegans, the first whole organism in which this question has been addressed. JF - Genome biology AU - Meyer, Joel N AU - Boyd, Windy A AU - Azzam, Gregory A AU - Haugen, Astrid C AU - Freedman, Jonathan H AU - Van Houten, Bennett AD - Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Alexander Drive, Research Triangle Park, NC 27709, USA. joel.meyer@duke.edu Y1 - 2007 PY - 2007 DA - 2007 SP - 1 VL - 8 IS - 5 KW - Index Medicus KW - Polymerase Chain Reaction KW - Animals KW - DNA Damage KW - Kinetics KW - Caenorhabditis elegans KW - Ultraviolet Rays -- adverse effects KW - DNA Repair -- physiology KW - Aging -- genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68095240?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Genome+biology&rft.atitle=Decline+of+nucleotide+excision+repair+capacity+in+aging+Caenorhabditis+elegans.&rft.au=Meyer%2C+Joel+N%3BBoyd%2C+Windy+A%3BAzzam%2C+Gregory+A%3BHaugen%2C+Astrid+C%3BFreedman%2C+Jonathan+H%3BVan+Houten%2C+Bennett&rft.aulast=Meyer&rft.aufirst=Joel&rft.date=2007-01-01&rft.volume=8&rft.issue=5&rft.spage=R70&rft.isbn=&rft.btitle=&rft.title=Genome+biology&rft.issn=1474-760X&rft_id=info:doi/ LA - 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Last updated - 2017-01-18 ER - TY - JOUR T1 - The Effect Of An International Embargo On Malnutrition And Childhood Mortality In Rural Haiti AN - 58767912; 2008-93109 AB - The study objective was to determine the effect of an international embargo against Haiti, from October 1991 through October 1994, on early childhood protein-energy malnutrition and all-cause mortality in a geographic area where humanitarian aid was continuously available to the children in the study. The authors used longitudinal anthropometric records on 1,593 children, 24 months old or younger, living in the rural Grand Anse Department of Haiti from 1989 through 1996. Kaplan-Meier graphs for all-cause mortality accounting for malnutrition status and stratified by calendar period were applied to the database and assessed using logrank tests. Adjusted relative risks were assessed by Cox regression. The results show that despite the continuous availability of preventive services (1989-1996), higher all-cause mortality was more strongly associated with a calendar period coinciding with the international embargo than with periods before and after the embargo. The incidence of childhood mortality and of severe malnutrition were also higher during the period of the embargo than in the periods before and after the embargo. The findings suggest that future international sanctions, even those with humanitarian/medical exceptions, could result in substantial infant death. Adapted from the source document. JF - International Journal of Health Services AU - Reid, Britt C AU - Psoter, Walter J AU - Gebrian, Bette AU - Wang, Min Qi AD - Epidemiology and Genetics Research Program, Division of Cancer Control and Population Sciences, National Cancer Institute, Executive Plaza North, Room 5104, 6130 Executive Blvd., MSC 7324, Bethesda, MD 20892-7324 reidbr@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 501 EP - 513 PB - Baywood Publishing, Amityville NY VL - 37 IS - 3 SN - 0020-7314, 0020-7314 KW - Trade and trade policy - Free trade and protection KW - Health conditions and policy - Diseases and disorders KW - Health conditions and policy - Food and nutrition KW - Population groups, population policy, and demographics - Children and youth KW - Population groups, population policy, and demographics - Demography and census KW - Law and ethics - International law KW - Social conditions and policy - Rural conditions KW - Haiti KW - Infant mortality KW - Mortality KW - Embargo KW - Sanctions (international law) KW - Malnutrition KW - Humanitarian law KW - Rural conditions KW - Children KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/58767912?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apais&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Health+Services&rft.atitle=The+Effect+Of+An+International+Embargo+On+Malnutrition+And+Childhood+Mortality+In+Rural+Haiti&rft.au=Reid%2C+Britt+C%3BPsoter%2C+Walter+J%3BGebrian%2C+Bette%3BWang%2C+Min+Qi&rft.aulast=Reid&rft.aufirst=Britt&rft.date=2007-01-01&rft.volume=37&rft.issue=3&rft.spage=501&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Health+Services&rft.issn=00207314&rft_id=info:doi/ LA - English DB - PAIS Index N1 - Date revised - 2008-02-04 N1 - Last updated - 2016-09-28 N1 - CODEN - IJHSC6 N1 - SubjectsTermNotLitGenreText - Embargo; Malnutrition; Haiti; Children; Mortality; Sanctions (international law); Humanitarian law; Rural conditions; Infant mortality ER - TY - JOUR T1 - Information needs and information seeking in a biomedical research setting: a study of scientists and science administrators. AN - 57704904; 200800657 AB - Objective: An information needs study of clinical specialists and biomedical researchers was conducted at the US National Institutes of Health (NIH) to inform library services and contribute to a broader understanding of information use in academic and research settings. Methods: A random stratified sample by job category of 500 NIH scientists was surveyed by telephone by an independent consultant using a standardized information industry instrument, augmented with locally developed questions. Results were analyzed for statistical significance using t-tests and chi square. Findings were compared with published studies and an aggregated dataset of information users in business, government, and health care from Outsell. Results: The study results highlighted similarities and differences with other studies and the industry standard, providing insights into user preferences, including new technologies. NIH scientists overwhelmingly used the NIH Library (424/500), began their searches at the library's Website rather than Google (P = < 0.001), were likely to seek information themselves (474/500), and valued desktop resources and services. Conclusion: While NIH staff work in a unique setting, they share some information characteristics with other researchers. The findings underscored the need to continue assessing specialized needs and seek innovative solutions. The study led to improvements or expansion of services such as developing a Website search engine, organizing gene sequence data, and assisting with manuscript preparation. Adapted from the source document. JF - Journal of the Medical Library Association (JMLA) AU - Grefsheim, Suzanne F AU - Rankin, Jocelyn A AD - Division of Library Services, National Institutes of Health, 10 Center Drive, MSC-1150, Bethesda, MD 20892 grefshes@nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 426 EP - 434 PB - Medical Library Association, Chicago, IL VL - 95 IS - 4 SN - 1536-5050, 1536-5050 KW - Scientists KW - User needs KW - Medicine KW - Information seeking behaviour KW - article KW - 4.14: USERS - OCCUPATIONAL GROUPS UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57704904?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.atitle=Information+needs+and+information+seeking+in+a+biomedical+research+setting%3A+a+study+of+scientists+and+science+administrators.&rft.au=Grefsheim%2C+Suzanne+F%3BRankin%2C+Jocelyn+A&rft.aulast=Grefsheim&rft.aufirst=Suzanne&rft.date=2007-01-01&rft.volume=95&rft.issue=4&rft.spage=426&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.issn=15365050&rft_id=info:doi/10.3163%2F1536-5050.95.4.426 L2 - http://www.mlanet.org/publications/jmla/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Information seeking behaviour; User needs; Medicine; Scientists DO - http://dx.doi.org/10.3163/1536-5050.95.4.426 ER - TY - JOUR T1 - HIV/AIDS information outreach: a community-based approach. AN - 57704782; 200801867 AB - Objective: The paper provides an overview of the National Library of Medicine's (NLM's) AIDS Community Information Outreach Program during the years 1994 to 2005, discusses the impact of previously funded projects, and explores future implications for HIV/AIDS information outreach to communities in need. Methods: A qualitative assessment was conducted to provide information on the impact of projects funded by the AIDS Community Information Outreach Program during fiscal year 2002. Interviews were conducted and final reports were analyzed, resulting in themes based on roles and responsibilities of participants and the impact of the projects in the communities. Results: Results from the assessment suggest that access to HIV/AIDS information led to improved communication between patients and their health care providers and encouraged better health care decision making. Feedback from reports and interviews included examples of impact such as an increase in services provided to communities, national and global recognition of HIV/AIDS services, sustainability of projects, and improved communication. Conclusion: Community-based health information outreach projects may empower the HIV/AIDS community to become more involved in health care and improve communication with providers. NLM will continue to promote the AIDS Community Information Outreach Program to encourage community organizations to design local projects for their specific communities. Adapted from the source document. JF - Journal of the Medical Library Association (JMLA) AU - Dancy, Nicole C AU - Dutcher, Gale A AD - Office of Outreach and Special Populations, Division of Specialized Information Services National Library of Medicine, 6707 Democracy Boulevard, Suite 510, Bethesda, MD 20892 dancyn1@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 323 EP - 329 PB - Medical Library Association, Chicago, IL VL - 95 IS - 3 SN - 1536-5050, 1536-5050 KW - National Library of Medicine, USA KW - HIV KW - Consumer health information KW - Community information services KW - article KW - 10.14: INFORMATION SERVICES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57704782?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.atitle=HIV%2FAIDS+information+outreach%3A+a+community-based+approach.&rft.au=Dancy%2C+Nicole+C%3BDutcher%2C+Gale+A&rft.aulast=Dancy&rft.aufirst=Nicole&rft.date=2007-01-01&rft.volume=95&rft.issue=3&rft.spage=323&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.issn=15365050&rft_id=info:doi/10.3163%2F1536-5050.95.3.323 L2 - http://www.mlanet.org/publications/jmla/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Community information services; Consumer health information; HIV; National Library of Medicine, USA DO - http://dx.doi.org/10.3163/1536-5050.95.3.323 ER - TY - JOUR T1 - The Central American Network for Disaster and Health Information. AN - 57703276; 200800691 AB - Purpose: This paper describes an international outreach program to support rebuilding Central America's health information infrastructure after several natural disasters in the region, including Hurricane Mitch in 1998 and two major earthquakes in 2001. Setting, Participants, and Description: The National Library of Medicine joined forces with the Pan American Health Organization /World Health Organization, the United Nations International Strategy for Disaster Reduction, and the Regional Center of Disaster Information for Latin America and the Caribbean (CRID) to strengthen libraries and information centers in Central America and improve the availability of and access to health and disaster information in the region by developing the Central American Network for Disaster and Health Information (CANDHI). Through CRID, the program created ten disaster health information centers in medical libraries and disaster-related organizations in six countries. Results/Outcome: This project served as a catalyst for the modernization of several medical libraries in Central America. The resulting CANDHI provides much needed electronic access to public health 'gray literature' on disasters, as well as access to numerous health information resources. CANDHI members assist their institutions and countries in a variety of disaster preparedness activities through collecting and disseminating information. Adapted from the source document. JF - Journal of the Medical Library Association (JMLA) AU - Arnesen, Stacey J AU - Cid, Victor H AU - Scott, John C AU - Perez, Ricardo AU - Zervaas, Dave AD - National Library of Medicine, 6707 Democracy Boulevard, Suite 510, Bethesda, MD 20892 stacey_arnesen@nlm.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 316 EP - 322 PB - Medical Library Association, Chicago, IL VL - 95 IS - 3 SN - 1536-5050, 1536-5050 KW - Disaster recovery KW - Consumer health information KW - Central America KW - Outreach services KW - article KW - 4.15: USER SERVICES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57703276?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.atitle=The+Central+American+Network+for+Disaster+and+Health+Information.&rft.au=Arnesen%2C+Stacey+J%3BCid%2C+Victor+H%3BScott%2C+John+C%3BPerez%2C+Ricardo%3BZervaas%2C+Dave&rft.aulast=Arnesen&rft.aufirst=Stacey&rft.date=2007-01-01&rft.volume=95&rft.issue=3&rft.spage=316&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.issn=15365050&rft_id=info:doi/10.3163%2F1536-5050.95.3.316 L2 - http://www.mlanet.org/publications/jmla/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Outreach services; Consumer health information; Disaster recovery; Central America DO - http://dx.doi.org/10.3163/1536-5050.95.3.316 ER - TY - JOUR T1 - Enviro-health links: environmental health subject guides from the National Library of Medicine. AN - 57702285; 200802117 AB - Staff at the National Library of Medicine create Internet subject guides that direct users to reliable sites on environmental health topics and provide pre-formulated searches of the National Library of Medicine's toxicology and environmental health databases. Criteria were developed to evaluate Web sites for authority, content, intended audience, and technical reliability. Searches are created using the most appropriate subject headings and keywords and can be limited to the most recent five years or to review articles only. Thirteen subject guides have been produced covering lead, arsenic, chemical and biological warfare, environmental justice, toxicogenomics, dietary supplements, and pesticides. Future topics include mercury and hazards from art materials. (Copies of this article are available for a fee from the Haworth Document Delivery Service, Haworth Press, Inc. E-Mail: getinfo@haworthpressinc.com, Web site http://www.HaworthPress.com). Adapted from the source document. COPIES ARE AVAILABLE FROM: HAWORTH DOCUMENT DELIVERY CENTER, The Haworth Press, Inc., 10 Alice Street, Binghamton, NY 13904-1580 JF - Journal of Electronic Resources in Medical Libraries AU - Publicker, Stephanie AD - Specialized Information Services, National Library of Medicine, 2 Democracy Plaza, Suite 510, 6707 Democracy Blvd., MSC 5467, Bethesda, MD 20892-5467 publicks@nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 1 EP - 9 PB - Haworth Press, Binghamton NY VL - 4 IS - 4 SN - 1542-4065, 1542-4065 KW - National Library of Medicine, USA KW - Environmental health KW - Searching KW - Subject indexing KW - article KW - 12.21: SUBJECT INDEXING UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57702285?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Electronic+Resources+in+Medical+Libraries&rft.atitle=Enviro-health+links%3A+environmental+health+subject+guides+from+the+National+Library+of+Medicine.&rft.au=Publicker%2C+Stephanie&rft.aulast=Publicker&rft.aufirst=Stephanie&rft.date=2007-01-01&rft.volume=4&rft.issue=4&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Journal+of+Electronic+Resources+in+Medical+Libraries&rft.issn=15424065&rft_id=info:doi/10.1300%2FJ383v04n04_01 LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Subject indexing; Searching; Environmental health; National Library of Medicine, USA DO - http://dx.doi.org/10.1300/J383v04n04_01 ER - TY - JOUR T1 - Use of the Internet to communicate with health care providers in the United States: estimates from the 2003 and 2005 Health Information National Trends Surveys (HINTS). AN - 57701010; 200801847 AB - Background: Despite substantial evidence that the public wants access to Internet-based communication with health care providers, online patient-provider communication remains relatively uncommon, and few studies have examined sociodemographic and health-related factors associated with the use of online communication with health care providers at a population level. Objective: The aim of the study was to use nationally representative data to report on the prevalence of and changes in use of online patient-provider communication in 2003 and 2005 and to describe sociodemographic and health-related factors associated with its use. Methods: Data for this study are from two iterations of the Health Information National Trends Survey (HINTS 2003, HINTS 2005). In both years, respondents were asked whether they had ever used email or the Internet to communicate with a doctor or a doctor's office. Adult Internet users in 2003 (n = 3982) and 2005 (n = 3244) were included in the present study. Multivariate logistic regression analysis was conducted to identify predictors for electronic communication with health care providers. Results: In 2003, 7% of Internet users had communicated online with an health care provider; this prevalence significantly increased to 10% in 2005. In multivariate analyses, Internet users with more years of education, who lived in a metro area, who reported poorer health status or who had a personal history of cancer were more likely to have used online patient-provider communication. Conclusions: Despite wide diffusion of the Internet, online patient-provider communication remains uncommon but is slowly increasing. Policy-level changes are needed to maximize the availability and effectiveness of online patient-provider communication for health care consumers and health care providers. Internet access remains a significant barrier to online patient-provider communication. Adapted from the source document. JF - Journal of Medical Internet Research AU - Burke Beckjord, Ellen AU - Finney Rutten, Lila J AU - Squiers, Linda AU - Arora, Neeraj K AU - Volckmann, Lindsey AU - Moser, Richard P AU - Hesse, Bradford W AD - National Cancer Institute, 6130 Executive Boulevard (EPN), 4051A, MSC 7365, Bethesda, MD 20892, USA beckjore@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 PB - Gunther Eysenbach MD MPH, Associate Professor, University of Toronto Senior Scientist, Centre for Global eHealth nnovation, Toronto, Canada VL - 9 IS - 3 SN - 1438-8871, 1438-8871 KW - Information communication KW - Consumer health information KW - Internet KW - Doctor-patient communication KW - article KW - 10.13: INFORMATION COMMUNICATION - SCIENCE, TECHNOLOGY, MEDICINE UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57701010?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Medical+Internet+Research&rft.atitle=Use+of+the+Internet+to+communicate+with+health+care+providers+in+the+United+States%3A+estimates+from+the+2003+and+2005+Health+Information+National+Trends+Surveys+%28HINTS%29.&rft.au=Burke+Beckjord%2C+Ellen%3BFinney+Rutten%2C+Lila+J%3BSquiers%2C+Linda%3BArora%2C+Neeraj+K%3BVolckmann%2C+Lindsey%3BMoser%2C+Richard+P%3BHesse%2C+Bradford+W&rft.aulast=Burke+Beckjord&rft.aufirst=Ellen&rft.date=2007-01-01&rft.volume=9&rft.issue=3&rft.spage=np&rft.isbn=&rft.btitle=&rft.title=Journal+of+Medical+Internet+Research&rft.issn=14388871&rft_id=info:doi/10.2196%2Fjmir.9.3.e20 L2 - http://www.jmir.org/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Doctor-patient communication; Information communication; Consumer health information; Internet DO - http://dx.doi.org/10.2196/jmir.9.3.e20 ER - TY - JOUR T1 - The Partners in Information Access for the Public Health Workforce: a collaboration to improve and protect the public's health, 1995-2006. AN - 57699811; 200801848 AB - Objective: The paper provides a complete accounting of the Partners in Information Access for the Public Health Workforce (Partners) initiative since its inception in 1997, including antecedent activities since 1995. Methods: A descriptive overview is provided that is based on a review of meeting summaries, published reports, Websites, project reports, databases, usage statistics, and personal experiences from offices in the National Library of Medicine (NLM), six organizations that collaborate formally with NLM on the Partners initiative, and one outside funding partner. Results: With ten years of experience, the initiative is an effective and unique public-private collaboration that builds on the strengths and needs of the organizations that are involved and the constituencies that they serve. Partners-supported and sponsored projects include satellite broadcasts or Webcasts, training initiatives, Web resource development, a collection of historical literature, and strategies for workforce enumeration and expansion of public health systems research, which provide excellent examples of the benefits realized from collaboration between the public health community and health sciences libraries. Conclusions: With continued funding, existing and new Partners-sponsored projects will be able to fulfill many public health information needs. This collaboration provides excellent opportunities to strengthen the partnership between library science and public health in the use of health information and tools for purposes of improving and protecting the public's health. Adapted from the source document. JF - Journal of the Medical Library Association (JMLA) AU - Cahn, Marjorie A AU - Auston, Ione AU - Selden, Catherine R AU - Cogdill, Keith AU - Baker, Stacy AU - Cavanaugh, Debra AU - Elliott, Sterling AU - Foster, Allison J AU - Leep, Carolyn J AU - Perez, Debra Joy AU - Pomietto, Blakely R AD - National Information Center for Health Services Research and Health Care Technology, National Library of Medicine, 8600 Rockville Pike, Bethesda, MD 20894 nichsr@nlm.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 301 EP - 309 PB - Medical Library Association, Chicago, IL VL - 95 IS - 3 SN - 1536-5050, 1536-5050 KW - Health professionals KW - Collaboration KW - Access to information KW - article KW - 10.13: INFORMATION COMMUNICATION - SCIENCE, TECHNOLOGY, MEDICINE UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57699811?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.atitle=The+Partners+in+Information+Access+for+the+Public+Health+Workforce%3A+a+collaboration+to+improve+and+protect+the+public%27s+health%2C+1995-2006.&rft.au=Cahn%2C+Marjorie+A%3BAuston%2C+Ione%3BSelden%2C+Catherine+R%3BCogdill%2C+Keith%3BBaker%2C+Stacy%3BCavanaugh%2C+Debra%3BElliott%2C+Sterling%3BFoster%2C+Allison+J%3BLeep%2C+Carolyn+J%3BPerez%2C+Debra+Joy%3BPomietto%2C+Blakely+R&rft.aulast=Cahn&rft.aufirst=Marjorie&rft.date=2007-01-01&rft.volume=95&rft.issue=3&rft.spage=301&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.issn=15365050&rft_id=info:doi/10.3163%2F1536-5050.95.3.301 L2 - http://www.mlanet.org/publications/jmla/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Access to information; Collaboration; Health professionals DO - http://dx.doi.org/10.3163/1536-5050.95.3.301 ER - TY - JOUR T1 - Building better connections: the National Library of Medicine and public health. AN - 57699773; 200800718 AB - Purpose: The paper describes the expansion of the public health programs and services of the National Library of Medicine (NLM) in the 1990s and provides the context in which NLM's public health outreach programs arose and exist today. Brief Description: Although NLM has always had collections and services relevant to public health, the US public health workforce made relatively little use of the library's information services and programs in the twentieth century. In the 1990s, intensified emphases on outreach to health professionals, building national information infrastructure, and promoting health data standards provided NLM with new opportunities to reach the public health community. A seminal conference cosponsored by NLM in 1995 produced an agenda for improving public health access to and use of advanced information technology and electronic information services. NLM actively pursued this agenda by developing new services and outreach programs and promoting public health informatics initiatives. Method: Historical analysis is presented. Results /Outcome: NLM took advantage of a propitious environment to increase visibility and understanding of public health information challenges and opportunities. The library helped create partnerships that produced new information services, outreach initiatives, informatics innovations, and health data policies that benefit the public health workforce and the diverse populations it serves. Adapted from the source document. JF - Journal of the Medical Library Association (JMLA) AU - Humphreys, Betsy L AD - National Library of Medicine, National Institutes of Health, US Department of Health and Human Services, 8600 Rockville Pike, Bethesda, MD 20894 betsy.humphreys@nih.hhs.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 293 EP - 300 PB - Medical Library Association, Chicago, IL VL - 95 IS - 3 SN - 1536-5050, 1536-5050 KW - National Library of Medicine, USA KW - Environmental health KW - Outreach services KW - article KW - 4.15: USER SERVICES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57699773?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.atitle=Building+better+connections%3A+the+National+Library+of+Medicine+and+public+health.&rft.au=Humphreys%2C+Betsy+L&rft.aulast=Humphreys&rft.aufirst=Betsy&rft.date=2007-01-01&rft.volume=95&rft.issue=3&rft.spage=293&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28JMLA%29&rft.issn=15365050&rft_id=info:doi/10.3163%2F1536-5050.95.3.293 L2 - http://www.mlanet.org/publications/jmla/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2008-05-15 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Outreach services; Environmental health; National Library of Medicine, USA DO - http://dx.doi.org/10.3163/1536-5050.95.3.293 ER - TY - JOUR T1 - Environmental health and toxicology resources of the United States National Library of Medicine. AN - 57697211; 00504671 AB - For over 40 years, the National Library of Medicine's (NLM) Toxicology and Environmental Health Information Program (TEHIP) has worked to organize and to provide access to an extensive array of environmental health and toxicology resources. During these years, the TEHIP program has evolved from a handful of databases developed primarily for researchers to a broad range of products and services that also serve industry, students, and the general public. TEHIP's resources include TOXNET(Registered) , a collection of databases, including online handbooks, bibliographic references, information on the release of chemicals in the environment, and a chemical dictionary. TEHIP also produces several resources aimed towards the general public, such as the Household Products Database , which helps users explore chemicals often found in common household products, and Tox Town(Registered) , an interactive guide to commonly encountered toxic substances, health, and the environment. This paper introduces some of NLM's environmental health and toxicology resources. (Copies of this article are available for a fee from the Haworth Document Delivery Service, Haworth Press, Inc. E-Mail: getinfo@haworthpressinc.com, Web site http://www.HaworthPress.com). (Author abstract) JF - Medical Reference Services Quarterly AU - Hochstein, Colette AU - Arnesen, Stacey AU - Goshorn, Jeanne AD - Division of Specialized Information Services (SIS), National Library of Medicine, 6707 Democracy Boulevard, Bethesda, MD 20892 colette@nlm.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 21 EP - 45 PB - Haworth Press Inc VL - 26 IS - 3 SN - 0276-3869, 0276-3869 KW - National Library of Medicine, USA KW - Online databases KW - Environmental health KW - Medical informatics KW - Toxicology KW - 10.13: INFORMATION COMMUNICATION - SCIENCE, TECHNOLOGY, MEDICINE UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57697211?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Medical+Reference+Services+Quarterly&rft.atitle=Environmental+health+and+toxicology+resources+of+the+United+States+National+Library+of+Medicine.&rft.au=Hochstein%2C+Colette%3BArnesen%2C+Stacey%3BGoshorn%2C+Jeanne&rft.aulast=Hochstein&rft.aufirst=Colette&rft.date=2007-01-01&rft.volume=26&rft.issue=3&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Medical+Reference+Services+Quarterly&rft.issn=02763869&rft_id=info:doi/10.1300%2FJ115v26n03_02 LA - English DB - Library & Information Science Abstracts (LISA) N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Medical informatics; Environmental health; Toxicology; Online databases; National Library of Medicine, USA DO - http://dx.doi.org/10.1300/J115v26n03_02 ER - TY - JOUR T1 - Empowering your institution through assessment. AN - 57687770; 00498322 AB - Objectives: The objectives of this study are to describe the process of linking Association of Academic Health Sciences Libraries (AAHSL) data with 2002 LibQUAL+ data and to address four analytical questions created by the AAHSL Task Force on Quality Assessment that relate both to user satisfaction and to services provided by AAHSL libraries. Methods: For the thirty-five AAHSL libraries that participated in the 2002 LibQUAL+ survey, nested-effect of variance was analyzed using a linear mixed model. Using the Pearson correlation coefficient, this study explored four questions about the effect of user demographics on perceived levels of satisfaction with library services. Results: The supposition that library user satisfaction may differ according to library institutional reporting structure was unsupported. Regarding effect on mean overall satisfaction, size of library staff is not significant (P = 0.860), number of constituents is slightly significant (P = 0.027), and ratio of staff to constituents has a moderate and significant effect (P = 0.004). Conclusions: From a demographic perspective, the 2002 LibQUAL+ survey represents the largest cross section of AAHSL libraries. Increased understanding of how qualitative assessment can supplement quantitative data supports evidence-based decision-making and practice. It also could promote changes in data collection and usage. (Author abstract) JF - Journal of the Medical Library Association ( JMLA ) AU - Joubert, Douglas J AU - Lee, Tamera P AD - National Institutes of Health, Library Bldg. 10 Room 1L09A, Bethesda, MD 20906-1150 joubertd@ors.od.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 46 EP - 53 PB - Medical Library Association VL - 95 IS - 1 SN - 1536-5050, 1536-5050 KW - Assessment KW - User services KW - Association of Academic Health Sciences Libraries KW - Service quality KW - Medical libraries KW - User satisfaction KW - 4.15: USER SERVICES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57687770?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Medical+Library+Association+%28+JMLA+%29&rft.atitle=Empowering+your+institution+through+assessment.&rft.au=Joubert%2C+Douglas+J%3BLee%2C+Tamera+P&rft.aulast=Joubert&rft.aufirst=Douglas&rft.date=2007-01-01&rft.volume=95&rft.issue=1&rft.spage=46&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Medical+Library+Association+%28+JMLA+%29&rft.issn=15365050&rft_id=info:doi/ LA - English DB - Library & Information Science Abstracts (LISA) N1 - Date revised - 2007-10-29 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - User services; User satisfaction; Medical libraries; Assessment; Service quality; Association of Academic Health Sciences Libraries ER - TY - JOUR T1 - DOCLINE(Registered): an overview of the DOCLINE system, its functions, purposes and descriptions of participating libraries. AN - 57664834; 00505035 AB - In March 2006, NLM released DOCLINE version 2.7 adding several major enhancements to the National Library of Medicine's interlibrary loan request routing and referral system. This paper presents an overview of the DOCLINE system, its functions, purposes, and descriptions of participating libraries. A summary of interlibrary loan statistics is also included. DOCLINE 2.7 provides new functionality to accommodate the growing shift to delivery of articles via electronic means. It also adds new features to request routing to provide a wider pool of potential lenders so that borrowers have access to the full resources of DOCLINE libraries. (Copies of this article are available for a fee from the Haworth Document Delivery Service, Haworth Press, Inc. E-Mail: getinfo@haworthpressinc.com, Web site http://www.HaworthPress.com). (Author abstract) JF - Journal of Interlibrary Loan, Document Supply and Electronic Reserve AU - Collins, Maria Elizabeth AD - DOCLINE Product Lead, National Library of Medicine, Public Services Division/Collection Access Section, 8600 Rockville Pike, Bethesda, MD 20894 maria_collins@nlm.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 15 EP - 28 PB - Haworth Press Inc VL - 17 IS - 3 SN - 1072-303X, 1072-303X KW - Software KW - National Library of Medicine, USA KW - DOCLINE KW - Medical libraries KW - Online document delivery KW - Interloans KW - 4.21: INTERLOANS AND PHOTOCOPYING SERVICES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57664834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Interlibrary+Loan%2C+Document+Supply+and+Electronic+Reserve&rft.atitle=DOCLINE%28Registered%29%3A+an+overview+of+the+DOCLINE+system%2C+its+functions%2C+purposes+and+descriptions+of+participating+libraries.&rft.au=Collins%2C+Maria+Elizabeth&rft.aulast=Collins&rft.aufirst=Maria&rft.date=2007-01-01&rft.volume=17&rft.issue=3&rft.spage=15&rft.isbn=&rft.btitle=&rft.title=Journal+of+Interlibrary+Loan%2C+Document+Supply+and+Electronic+Reserve&rft.issn=1072303X&rft_id=info:doi/10.1300%2FJ474v17n03_05 LA - English DB - Library & Information Science Abstracts (LISA) N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Interloans; Online document delivery; Software; Medical libraries; DOCLINE; National Library of Medicine, USA DO - http://dx.doi.org/10.1300/J474v17n03_05 ER - TY - JOUR T1 - The PubMed Central Archive and the back issue scanning project. AN - 57648225; 00505044 AB - This paper is a description of the National Library of Medicine's PubMed Central. PubMed Central comprises back issue scanning and digitization project of scanned back issues journals from the mid 1800s and current content from publishers. The National Library of Medicine's PubMed Central Archive is growing by thousands of articles per month. The back issues now account for two-thirds of the total archive of about 720,000 articles from 210 journals. (Copies of this article are available for a fee from the Haworth Document Delivery Service, Haworth Press, Inc. E-Mail: getinfo@haworthpressinc.com, Web site http://www.HaworthPress.com). (Author abstract) JF - Journal of Interlibrary Loan, Document Supply and Electronic Reserve AU - Fishel, Martha AU - Myers, Carol J AD - National Library of Medicine, 8066 Rockville Pike, Bethesda, MD 20894 martha.fishel@nlm.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 109 EP - 116 PB - Haworth Press Inc VL - 17 IS - 3 SN - 1072-303X, 1072-303X KW - Open access KW - National Library of Medicine, USA KW - PubMed Central KW - Electronic publishing KW - Digital archives KW - 3.2: ARCHIVES UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57648225?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Alisa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Interlibrary+Loan%2C+Document+Supply+and+Electronic+Reserve&rft.atitle=The+PubMed+Central+Archive+and+the+back+issue+scanning+project.&rft.au=Fishel%2C+Martha%3BMyers%2C+Carol+J&rft.aulast=Fishel&rft.aufirst=Martha&rft.date=2007-01-01&rft.volume=17&rft.issue=3&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=Journal+of+Interlibrary+Loan%2C+Document+Supply+and+Electronic+Reserve&rft.issn=1072303X&rft_id=info:doi/10.1300%2FJ474v17n03_14 LA - English DB - Library & Information Science Abstracts (LISA) N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Digital archives; Electronic publishing; Open access; PubMed Central; National Library of Medicine, USA DO - http://dx.doi.org/10.1300/J474v17n03_14 ER - TY - JOUR T1 - Adolescents with Conduct Disorder: Early Smoking and Treatment Requests AN - 57288156; 200913658 AB - This study explored the relationship of a diagnosis of conduct disorder (CD) with the developmental smoking trajectory among 117 adolescent volunteers. Logistic regression analyses revealed that adolescents with CD smoked their first whole cigarette earlier (p = 0.03) and sought cessation treatment earlier (p = .01) compared to non-CD adolescents. Additionally, adolescents who smoked their first whole cigarette before the age of nine were eight times more likely to have CD. These findings suggest that in addition to addressing disruptive behaviors, early prevention and access to interventions for tobacco use are needed for youths with CD. Adapted from the source document. JF - The American Journal on Addictions AU - Bagot, Kara S AU - Berarducci, Jennifer M AU - Franken, Frederick H AU - Frazier, Matthew J AU - Ernst, Monique AU - Moolchan, Eric T AD - The National Institute on Drug Abuse, Intramural Research Program, Baltimore, Maryland Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 62 EP - 66 PB - Informa Healthcare, Taylor & Francis, New York NY VL - 16 IS - 1 SN - 1055-0496, 1055-0496 KW - Smoking KW - Initiation KW - Cessation KW - Conduct disordered adolescents KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57288156?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+American+Journal+on+Addictions&rft.atitle=Adolescents+with+Conduct+Disorder%3A+Early+Smoking+and+Treatment+Requests&rft.au=Bagot%2C+Kara+S%3BBerarducci%2C+Jennifer+M%3BFranken%2C+Frederick+H%3BFrazier%2C+Matthew+J%3BErnst%2C+Monique%3BMoolchan%2C+Eric+T&rft.aulast=Bagot&rft.aufirst=Kara&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=62&rft.isbn=&rft.btitle=&rft.title=The+American+Journal+on+Addictions&rft.issn=10550496&rft_id=info:doi/10.1080%2F10550490601080100 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2009-07-06 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Smoking; Cessation; Conduct disordered adolescents; Initiation DO - http://dx.doi.org/10.1080/10550490601080100 ER - TY - JOUR T1 - Infant childcare settings and the development of gender-specific adaptive behaviors AN - 57253684; 200815817 AB - In this study, we defined three distinct groups based on the infant's principal childcare experience: infants reared exclusively at home by their mothers, infants reared in their own homes but by a non-familial childcare provider, and infants reared in non-familial homes in group care. At 4.5 years of age, we compared mothers' and teachers' independent views of the communication, daily living, socialization and motor adaptive behaviors of girls and boys with these different infant childcare histories, after taking multiple family selection factors into consideration. Boys who had other-home-group-care in infancy expressed lower levels of overall adaptive functioning, as well as communication, daily living and socialization skills, than girls. Girls with other-home-group-care in infancy had better adaptive daily living and socialization skills than girls who had maternal care. Different infant childcare experiences appear to predict different adaptive behaviors in boys and girls. Adapted from the source document. JF - Early Child Development and Care AU - Bornstein, Marc H AU - Hahn, Chun-Shin AD - National Institute of Child Health and Human Development, Maryland, USA Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 15 EP - 41 PB - Routledge/Taylor & Francis, UK VL - 177 IS - 1 SN - 0300-4430, 0300-4430 KW - Day care centres KW - Mother-Infant interactions KW - Gender differences KW - Child development KW - Child care KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57253684?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Early+Child+Development+and+Care&rft.atitle=Infant+childcare+settings+and+the+development+of+gender-specific+adaptive+behaviors&rft.au=Bornstein%2C+Marc+H%3BHahn%2C+Chun-Shin&rft.aulast=Bornstein&rft.aufirst=Marc&rft.date=2007-01-01&rft.volume=177&rft.issue=1&rft.spage=15&rft.isbn=&rft.btitle=&rft.title=Early+Child+Development+and+Care&rft.issn=03004430&rft_id=info:doi/10.1080%2F03004430500317192 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-08-04 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Gender differences; Child care; Mother-Infant interactions; Day care centres; Child development DO - http://dx.doi.org/10.1080/03004430500317192 ER - TY - JOUR T1 - Russian Alcohol Policy In The Making AN - 57250595; 200816261 AB - Aims: This paper examines implementation of the 2005 federal alcohol control law in the Russian Federation. Methods: The documents on the Russian Federation federal legislation on the control of the production and turnover of ethyl alcohol, and ethyl alcohol containing products, news reports, research, and historical documents were gathered and analysed for implementation barriers. Results: Consumption of alcoholic beverages, especially spirits, has been one the most significant public health problems in Russia for many centuries. Prior attempts to control alcohol consumption have been unsuccessful, in part due to the government's reliance on alcohol revenue, and its inability to implement creative and manageable solutions in the light of the high drinking rates. Implementation of this legislation has been a challenge in Russia because of administrative oversight, lack of organizational preparation, and corruption. Conclusions: The law discussed in this paper presented a window of opportunity to ameliorate the deteriorating health status and reverse the impending mortality crisis. However, a number of barriers presented substantial setbacks toward realization of this legislation. Adapted from the source document. JF - Alcohol and Alcoholism AU - Levintova, Marya AD - Fogarty International Center National Institutes of Health 31 Center Drive, Room B2C11 Bethesda, MD 20892 levintovam@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 500 EP - 505 PB - Oxford University Press, UK VL - 42 IS - 5 SN - 0735-0414, 0735-0414 KW - Alcohol consumption KW - Health policy KW - Russian Federation KW - Legislation KW - Public health KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57250595?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Alcohol+and+Alcoholism&rft.atitle=Russian+Alcohol+Policy+In+The+Making&rft.au=Levintova%2C+Marya&rft.aulast=Levintova&rft.aufirst=Marya&rft.date=2007-01-01&rft.volume=42&rft.issue=5&rft.spage=500&rft.isbn=&rft.btitle=&rft.title=Alcohol+and+Alcoholism&rft.issn=07350414&rft_id=info:doi/10.1093%2Falcalc%2Fagm040 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-08-04 N1 - Last updated - 2016-09-27 N1 - CODEN - ALALDD N1 - SubjectsTermNotLitGenreText - Public health; Russian Federation; Alcohol consumption; Health policy; Legislation DO - http://dx.doi.org/10.1093/alcalc/agm040 ER - TY - JOUR T1 - Research Review: A neuroscience framework for pediatric anxiety disorders AN - 57233310; 200807907 AB - Across a range of mammalian species, early developmental variations in fear-related behaviors constrain patterns of anxious behavior throughout life. Individual differences in anxiety among rodents and non-human primates have been shown to reflect early-life influences of genes and the environment on brain circuitry. However, in humans, the manner in which genes and the environment developmentally shape individual differences in anxiety and associated brain circuitry remains poorly specified. The current review presents a conceptual framework that facilitates clinical research examining developmental influences on brain circuitry and anxiety. Research using threat-exposure paradigms might most directly integrate basic and clinical perspectives on pediatric anxiety. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Pine, Daniel S AD - Section on Development and Affective Neuroscience, National Institute of Mental Health Intramural Research Program, Bethesda, MD, USA daniel.pine@nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 631 EP - 648 PB - Blackwell Publishing, Oxford UK VL - 48 IS - 7 SN - 0021-9630, 0021-9630 KW - Paediatrics KW - Individual differences KW - Anxiety disorders KW - Genes KW - Neurosciences KW - Brain KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57233310?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Research+Review%3A+A+neuroscience+framework+for+pediatric+anxiety+disorders&rft.au=Pine%2C+Daniel+S&rft.aulast=Pine&rft.aufirst=Daniel&rft.date=2007-01-01&rft.volume=48&rft.issue=7&rft.spage=631&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2007.01751.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-04-02 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Brain; Paediatrics; Genes; Individual differences; Anxiety disorders; Neurosciences DO - http://dx.doi.org/10.1111/j.1469-7610.2007.01751.x ER - TY - JOUR T1 - Incentive-related modulation of cognitive control in healthy, anxious, and depressed adolescents: development and psychopathology related differences AN - 57230962; 200806772 AB - Background: Developmental changes in cognitive and affective processes contribute to adolescent risk-taking behavior, emotional intensification, and psychopathology. The current study examined adolescent development of cognitive control processes and their modulation by incentive, in health and psychopathology. Predictions include 1) better cognitive control in adults than adolescents, and in healthy adolescents than anxious and depressed adolescents, and 2) a stronger influence of incentives in adolescents than adults, and in healthy adolescents than their depressed and anxious counterparts. Methods: Antisaccadic eye movement parameters, which provide a measure of cognitive control, were collected during a reward antisaccade task that included parameterized incentive levels. Participants were 20 healthy adults, 30 healthy adolescents, 16 adolescents with an anxiety disorder, and 11 adolescents with major depression. Performance accuracy and saccade latency were analyzed to test both developmental and psychopathology hypotheses. Results: Development and psychopathology group differences in cognitive control were found. Specifically, adults performed better than healthy adolescents, and healthy adolescents than anxious and depressed adolescents. Incentive improved accuracy for all groups; however, incremental increases were not sufficiently large to further modulate performance. Incentives also affected saccade latencies, pushing healthy adolescent latencies to adult levels, while being less effective in adolescents with depression or anxiety. This latter effect was partially mediated by anxiety symptom severity. Conclusions: Current findings evidence the modulation of cognitive control processes by incentives. While seen in both healthy adults and healthy adolescents, this modulatory effect was stronger in youth. While anxious and depressed adolescents exhibited improved cognitive control under incentives, this effect was smaller than that in healthy adolescents. These findings suggest differential incentive and/or cognitive control processing in anxiety and depression, and across development. Differences could result from disorder specific, or combined developmental and pathological mechanisms. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Hardin, Michael G AU - Schroth, Elizabeth AU - Pine, Daniel S AU - Ernst, Monique AD - Emotional Development and Affective Neuroscience Branch, Mood and Anxiety Disorders Program, National Institute of Mental Health, NIH /DHHS, USA hardinm@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 446 EP - 454 PB - Blackwell Publishing, Oxford UK VL - 48 IS - 5 SN - 0021-9630, 0021-9630 KW - Depression KW - Anxiety KW - Executive control KW - Psychopathology KW - Incentives KW - Adolescents KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57230962?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Incentive-related+modulation+of+cognitive+control+in+healthy%2C+anxious%2C+and+depressed+adolescents%3A+development+and+psychopathology+related+differences&rft.au=Hardin%2C+Michael+G%3BSchroth%2C+Elizabeth%3BPine%2C+Daniel+S%3BErnst%2C+Monique&rft.aulast=Hardin&rft.aufirst=Michael&rft.date=2007-01-01&rft.volume=48&rft.issue=5&rft.spage=446&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2006.01722.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-04-02 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Executive control; Incentives; Adolescents; Anxiety; Psychopathology; Depression DO - http://dx.doi.org/10.1111/j.1469-7610.2006.01722.x ER - TY - JOUR T1 - Enhancing employee capacity to prioritize health insurance benefits AN - 57230026; 200809953 AB - Objective: To demonstrate that employees can gain understanding of the financial constraints involved in designing health insurance benefits. Background: While employees who receive their health insurance through the workplace have much at stake as the cost of health insurance rises, they are not necessarily prepared to constructively participate in prioritizing their health insurance benefits in order to limit cost. Design: Structured group exercises. Setting and participants: Employees of 41 public and private organizations in Northern California. Intervention: Administration of the CHAT (Choosing Healthplans All Together) exercise in which participants engage in deliberation to design health insurance benefits under financial constraints. Main outcome measures: Change in priorities and attitudes about the need to exercise insurance cost constraints. Results: Participants ( N =744) became significantly more cognizant of the need to limit insurance benefits for the sake of affordability and capable of prioritizing benefit options. Those agreeing that it is reasonable to limit health insurance coverage given the cost increased from 47% to 72%. Conclusion: It is both possible and valuable to involve employees in priority setting regarding health insurance benefits through the use of structured decision tools. Adapted from the source document. JF - Health Expectations AU - Danis, Marion AU - Goold, Susan Dorr AU - Parise, Carol AU - Ginsburg, Marjorie AD - Head, Section on Ethics and Health Policy, National Institutes of Health, Bethesda, MD, USA mdanis@nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 236 EP - 247 PB - Blackwell Publishing, Oxford UK VL - 10 IS - 3 SN - 1369-6513, 1369-6513 KW - Affordability KW - Prioritizing KW - Priorities KW - Health insurance KW - Exercise KW - Workplaces KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57230026?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Health+Expectations&rft.atitle=Enhancing+employee+capacity+to+prioritize+health+insurance+benefits&rft.au=Danis%2C+Marion%3BGoold%2C+Susan+Dorr%3BParise%2C+Carol%3BGinsburg%2C+Marjorie&rft.aulast=Danis&rft.aufirst=Marion&rft.date=2007-01-01&rft.volume=10&rft.issue=3&rft.spage=236&rft.isbn=&rft.btitle=&rft.title=Health+Expectations&rft.issn=13696513&rft_id=info:doi/10.1111%2Fj.1369-7625.2007.00442.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-05-02 N1 - Last updated - 2016-09-27 N1 - CODEN - HEHPFM N1 - SubjectsTermNotLitGenreText - Health insurance; Exercise; Prioritizing; Priorities; Affordability; Workplaces DO - http://dx.doi.org/10.1111/j.1369-7625.2007.00442.x ER - TY - JOUR T1 - THE ALCOHOL DEPENDENCE SYNDROME, 30YEARS LATER-A RESPONSE TO THE COMMENTARIES AN - 57227557; 200807348 AB - Abstract not provided by publisher. JF - Addiction AU - Li, Ting-Kai AU - Hewitt, Brenda G AU - Grant, Bridget F AD - National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892-9304, USA. : Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 1537 EP - 1538 PB - Blackwell Publishing, Oxford UK VL - 102 IS - 10 SN - 0965-2140, 0965-2140 KW - Alcohol dependence KW - Alcohol related disorders KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57227557?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Addiction&rft.atitle=THE+ALCOHOL+DEPENDENCE+SYNDROME%2C+30YEARS+LATER-A+RESPONSE+TO+THE+COMMENTARIES&rft.au=Li%2C+Ting-Kai%3BHewitt%2C+Brenda+G%3BGrant%2C+Bridget+F&rft.aulast=Li&rft.aufirst=Ting-Kai&rft.date=2007-01-01&rft.volume=102&rft.issue=10&rft.spage=1537&rft.isbn=&rft.btitle=&rft.title=Addiction&rft.issn=09652140&rft_id=info:doi/10.1111%2Fj.1360-0443.2007.02005.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-04-02 N1 - Last updated - 2016-09-27 N1 - CODEN - ADICE5 N1 - SubjectsTermNotLitGenreText - Alcohol related disorders; Alcohol dependence DO - http://dx.doi.org/10.1111/j.1360-0443.2007.02005.x ER - TY - JOUR T1 - A new perspective on the effects of price promotions in Taiwan: a longitudinal study of a Chinese society AN - 57226387; 200809967 AB - This study investigated the effects of price promotions on consumers' brand affect. Given the inconsistent findings in previous research, it is proposed that the effects of price promotion depend on two moderator variables: brand image and consumer loyalty. For high loyalty consumers of a prestigious brand, price incentive incompatible with the brand image can hurt the brand affect. When a non-prestigious brand is involved, brand affect is positively influenced. However, these effects are limited to high loyalty consumers only. There was no effect on low loyalty consumers. In a longitudinal study of the Taiwanese market using emails, these hypotheses were tested and supported. Adapted from the source document. JF - International Journal of Consumer Studies AU - Shen, Yung-Cheng AU - Chi, Chung-Hsing AU - Chen, Ja-Shen AD - Department of Business Administration, Yuan-ze University, Nei-Li, Taoyuan, Taiwan Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 385 EP - 390 PB - Blackwell Publishing, Malden, MA VL - 31 IS - 4 SN - 1470-6423, 1470-6423 KW - Promotion KW - Prices KW - Moderator variables KW - Consumers KW - Loyalty KW - Brands KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57226387?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=International+Journal+of+Consumer+Studies&rft.atitle=A+new+perspective+on+the+effects+of+price+promotions+in+Taiwan%3A+a+longitudinal+study+of+a+Chinese+society&rft.au=Shen%2C+Yung-Cheng%3BChi%2C+Chung-Hsing%3BChen%2C+Ja-Shen&rft.aulast=Shen&rft.aufirst=Yung-Cheng&rft.date=2007-01-01&rft.volume=31&rft.issue=4&rft.spage=385&rft.isbn=&rft.btitle=&rft.title=International+Journal+of+Consumer+Studies&rft.issn=14706423&rft_id=info:doi/10.1111%2Fj.1470-6431.2006.00569.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-05-02 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Brands; Consumers; Loyalty; Prices; Promotion; Moderator variables DO - http://dx.doi.org/10.1111/j.1470-6431.2006.00569.x ER - TY - JOUR T1 - Dynamic mapping of cortical development before and after the onset of pediatric bipolar illness AN - 57219776; 200806634 AB - Background: There are, to date, no pre-post onset longitudinal imaging studies of bipolar disorder at any age. We report the first prospective study of cortical brain development in pediatric bipolar illness for 9 male children, visualized before and after illness onset. Method:We contrast this pattern with that observed in a matched group of healthy children as well as in a matched group of 8 children with 'atypical psychosis' who had similar initial presentation marked by mood dysregulation and transient psychosis (labeled as 'multi-dimensionally impaired' (MDI)) as in the bipolar group, but have not, to date, developed bipolar illness. Results: Dynamic maps, reconstructed by applying novel cortical pattern matching algorithms, for the children who became bipolar I showed subtle, regionally specific, bilaterally asymmetrical cortical changes. Cortical GM increased over the left temporal cortex and decreased bilaterally in the anterior (and sub genual) cingulate cortex. This was seen most strikingly after the illness onset, and showed a pattern distinct from that seen in childhood onset schizophrenia. The bipolar neurodevelopmental trajectory was generally shared by the children who remained with MDI diagnosis without converting to bipolar I, suggesting that this pattern of cortical development may reflect affective dysregulation (lability) in general. Conclusions: These dynamic trajectories of cortical development may explain age-related disparate findings from cross-sectional studies of bipolar illness, and suggest the importance of mood disordered non-bipolar control group in future studies. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Gogtay, Nitin AU - Ordonez, Anna AU - Herman, David H AU - Hayashi, Kiralee M AU - Greenstein, Deanna AU - Vaituzis, Cathy AU - Lenane, Marge AU - Clasen, Liv AU - Sharp, Wendy AU - Giedd, Jay N AU - Jung, David AU - Nugent, Tom F, III AU - Toga, Arthur W AU - Leibenluft, Ellen AU - Thompson, Paul M AU - Rapoport, Judith L AD - Child Psychiatry Branch gogtayn@intra.nimh.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 852 EP - 862 PB - Blackwell Publishing, Oxford UK VL - 48 IS - 9 SN - 0021-9630, 0021-9630 KW - Onset KW - Affective disorders KW - Bipolar affective disorder KW - Brain imaging KW - Children KW - Cortical malformation KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57219776?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Dynamic+mapping+of+cortical+development+before+and+after+the+onset+of+pediatric+bipolar+illness&rft.au=Gogtay%2C+Nitin%3BOrdonez%2C+Anna%3BHerman%2C+David+H%3BHayashi%2C+Kiralee+M%3BGreenstein%2C+Deanna%3BVaituzis%2C+Cathy%3BLenane%2C+Marge%3BClasen%2C+Liv%3BSharp%2C+Wendy%3BGiedd%2C+Jay+N%3BJung%2C+David%3BNugent%2C+Tom+F%2C+III%3BToga%2C+Arthur+W%3BLeibenluft%2C+Ellen%3BThompson%2C+Paul+M%3BRapoport%2C+Judith+L&rft.aulast=Gogtay&rft.aufirst=Nitin&rft.date=2007-01-01&rft.volume=48&rft.issue=9&rft.spage=852&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2007.01747.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2015-12-01 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Cortical malformation; Brain imaging; Bipolar affective disorder; Children; Affective disorders; Onset DO - http://dx.doi.org/10.1111/j.1469-7610.2007.01747.x ER - TY - JOUR T1 - The Impact Of Individual Ability, Favorable Team Member Scores, And Student Perception Of Course Importance On Student Preference Of Team-Based Learning And Grading Methods AN - 57215429; 200808430 AB - This study explores the impact of individual ability and favorable team member scores on student preference of team-based learning and grading methods, and examines the moderating effects of student perception of course importance on student preference of team-based learning and grading methods. The author also investigates the relationship between student perception of course importance and their responses to social loafing. Results indicate that individual ability on the preference of team-based learning was affected by the three levels of favorable team member scores. For students with a low level of individual ability, the preference for team-based learning was significant among students with each of three levels of favorable team member scores (p less than .05). However, the team-based learning and grading methods was not significant (p greater than .05). The findings also reveal a negative correlation between student perception of course importance and their responses to social loafing (p less than .05). Findings note the importance of teachers' grading methods, student perceptions of course importance as well as individual ability and favorable team member scores in the team selection process to promote student attitude toward team-based learning. Adapted from the source document. JF - Adolescence AU - Su, Allan Yen-Lun AD - Department of Hospitality and Recreation Management, National Pingtung University of Science and Technology, 1, Hseuh Fu Road, Nei-Pu, Pingtung 91201, Taiwan Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 805 EP - 826 PB - Libra Publishers, San Diego CA VL - 42 IS - 168 SN - 0001-8449, 0001-8449 KW - Groups KW - Learning KW - Teaching KW - Performance KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57215429?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Adolescence&rft.atitle=The+Impact+Of+Individual+Ability%2C+Favorable+Team+Member+Scores%2C+And+Student+Perception+Of+Course+Importance+On+Student+Preference+Of+Team-Based+Learning+And+Grading+Methods&rft.au=Su%2C+Allan+Yen-Lun&rft.aulast=Su&rft.aufirst=Allan&rft.date=2007-01-01&rft.volume=42&rft.issue=168&rft.spage=805&rft.isbn=&rft.btitle=&rft.title=Adolescence&rft.issn=00018449&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-04-02 N1 - Last updated - 2016-09-27 N1 - CODEN - ADOLAO N1 - SubjectsTermNotLitGenreText - Learning; Groups; Teaching; Performance ER - TY - JOUR T1 - Specificity of facial expression labeling deficits in childhood psychopathology AN - 57197323; 200806725 AB - Background: We examined whether face-emotion labeling deficits are illness-specific or an epiphenomenon of generalized impairment in pediatric psychiatric disorders involving mood and behavioral dysregulation. Method: Two hundred fifty-two youths (7-18 years old) completed child and adult facial expression recognition subtests from the Diagnostic Analysis of Nonverbal Accuracy (DANVA) instrument. Forty-two participants had bipolar disorder (BD), 39 had severe mood dysregulation (SMD; i.e., chronic irritability, hyperarousal without manic episodes), 44 had anxiety and/or major depressive disorders (ANX/MDD), 35 had attention-deficit/hyperactivity and/or conduct disorder (ADHD/CD), and 92 were controls. Dependent measures were number of errors labeling happy, angry, sad, or fearful emotions. Results: BD and SMD patients made more errors than ANX/MDD, ADHD/CD, or controls when labeling adult or child emotional expressions. BD and SMD patients did not differ in their emotion-labeling deficits. Conclusions: Face-emotion labeling deficits differentiate BD and SMD patients from patients with ANX/MDD or ADHD/CD and controls. The extent to which such deficits cause vs. result from emotional dysregulation requires further study. Adapted from the source document. JF - The Journal of Child Psychology and Psychiatry AU - Guyer, Amanda E AU - McClure, Erin B AU - Adler, Abby D AU - Brotman, Melissa A AU - Rich, Brendan A AU - Kimes, Alane S AU - Pine, Daniel S AU - Ernst, Monique AU - Leibenluft, Ellen AD - Mood and Anxiety Program (MAP), National Institute of Mental Health (NIMH), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Bethesda, MD, USA amandaguyer@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 863 EP - 871 PB - Blackwell Publishing, Oxford UK VL - 48 IS - 9 SN - 0021-9630, 0021-9630 KW - Labelling KW - Facial expressions KW - Affective disorders KW - Attention deficit hyperactivity disorder KW - Emotion recognition KW - Children KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57197323?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+Child+Psychology+and+Psychiatry&rft.atitle=Specificity+of+facial+expression+labeling+deficits+in+childhood+psychopathology&rft.au=Guyer%2C+Amanda+E%3BMcClure%2C+Erin+B%3BAdler%2C+Abby+D%3BBrotman%2C+Melissa+A%3BRich%2C+Brendan+A%3BKimes%2C+Alane+S%3BPine%2C+Daniel+S%3BErnst%2C+Monique%3BLeibenluft%2C+Ellen&rft.aulast=Guyer&rft.aufirst=Amanda&rft.date=2007-01-01&rft.volume=48&rft.issue=9&rft.spage=863&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+Child+Psychology+and+Psychiatry&rft.issn=00219630&rft_id=info:doi/10.1111%2Fj.1469-7610.2007.01758.x LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-04-02 N1 - Last updated - 2016-09-27 N1 - CODEN - JPPDAI N1 - SubjectsTermNotLitGenreText - Attention deficit hyperactivity disorder; Affective disorders; Facial expressions; Children; Labelling; Emotion recognition DO - http://dx.doi.org/10.1111/j.1469-7610.2007.01758.x ER - TY - JOUR T1 - Four Paradigms of Clinical Research and Research Oversight AN - 57160841; 200708930 AB - Delineates four periods & attendant paradigms of clinical biomedical research & ethical oversight in the US from the early 1940s through the mid 1990s: researcher paternalism, regulatory protectionism, participant access, & community partnership. Overlaps between paradigms & scandals or crises marking the transition from one paradigm to the next are described & directions for the future are discussed. Tables. K. Hyatt Stewart JF - Cambridge Quarterly of Healthcare Ethics AU - Emanuel, Ezekiel J AU - Grady, Christine AD - W.G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 82 EP - 96 PB - Cambridge University Press, New York NY VL - 16 IS - 1 SN - 0963-1801, 0963-1801 KW - Clinical research KW - Biomedicine KW - Historical perspectives KW - Paternalism KW - Models KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57160841?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cambridge+Quarterly+of+Healthcare+Ethics&rft.atitle=Four+Paradigms+of+Clinical+Research+and+Research+Oversight&rft.au=Emanuel%2C+Ezekiel+J%3BGrady%2C+Christine&rft.aulast=Emanuel&rft.aufirst=Ezekiel&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=82&rft.isbn=&rft.btitle=&rft.title=Cambridge+Quarterly+of+Healthcare+Ethics&rft.issn=09631801&rft_id=info:doi/10.1017%2FS0963180107070090 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2007-06-01 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Historical perspectives; Clinical research; Models; Biomedicine; Paternalism DO - http://dx.doi.org/10.1017/S0963180107070090 ER - TY - JOUR T1 - A Cross-National Comparison of Youth Risk Behaviors in Latino Secondary School Students Living in El Salvador and the USA AN - 57151851; 200711990 AB - Objectives. As Latin Americans' exposure to the USA increases through migration patterns and US political and economic ties to their countries of origin, they become susceptible to adopting not only the cultural expressions of the USA such as fashion, but also the health-related behaviors of the US population. In assessing potential health risks for Salvadoran youth that may result from the connection between Latin Americans and the USA, this study compared the prevalence of health risk behaviors from four behavior domains (aggression and victimization, depression and suicidal ideation, substance use, and sexual behavior) between Salvadoran and US Latino secondary school students aged 14-17 years. Design. A secondary analysis was performed on two 1999 cross-sectional survey data. In the USA, results were based on 1,063 Latino high school students who answered the nationally representative Youth Risk Behavior Survey (YRBS) conducted by the Centers for Disease Control and Prevention. In El Salvador, results were based on 793 public secondary school students who answered a local YRBS survey conducted in coordination with the Ministry of Education of El Salvador. Results. The prevalence rates for aggression/victimization and for depression and suicidal ideation behaviors were similar between Salvadoran and US Latino adolescents. Substance use prevalence, however, was 10-40% higher for US Latino adolescents. While the prevalence of sexual intercourse was higher among US Latino youth (between 13 and 27% higher, depending on age), the prevalence of condom use was lower among sexually active Salvadoran youth (between 11 and 42% lower, depending on age). Conclusions. In the context of the transnationalization of the Salvadoran population, with potential for increased influence of the USA in Salvadoran culture, these differences in risk behavior are important for targeting effective interventions for Latino adolescents in El Salvador and in the USA. Adapted from the source document. JF - Ethnicity & Health AU - Springer, Andrew AU - Kelder, Steve AU - Orpinas, Pamela AU - Baumler, Elizabeth AD - National Cancer Institute Postdoctoral Fellow, The U Texas School Public Health, Center Health Promotion & Prevention Research, Houston, TX Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 69 EP - 88 PB - Taylor & Francis, Abingdon UK VL - 12 IS - 1 SN - 1355-7858, 1355-7858 KW - Adolescents, Risk Behavior, El Salvador, Latin America, Cross-National, Suicide, Aggression, Drugs, Sexual Behaviors KW - Latin American people KW - Risk behaviour KW - El Salvadorean people KW - Drug abuse KW - Adolescents KW - Health behaviour KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57151851?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Ethnicity+%26+Health&rft.atitle=A+Cross-National+Comparison+of+Youth+Risk+Behaviors+in+Latino+Secondary+School+Students+Living+in+El+Salvador+and+the+USA&rft.au=Springer%2C+Andrew%3BKelder%2C+Steve%3BOrpinas%2C+Pamela%3BBaumler%2C+Elizabeth&rft.aulast=Springer&rft.aufirst=Andrew&rft.date=2007-01-01&rft.volume=12&rft.issue=1&rft.spage=69&rft.isbn=&rft.btitle=&rft.title=Ethnicity+%26+Health&rft.issn=13557858&rft_id=info:doi/10.1080%2F13557850601002155 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2007-07-02 N1 - Last updated - 2016-09-27 N1 - CODEN - ETHEFR N1 - SubjectsTermNotLitGenreText - Latin American people; Adolescents; Risk behaviour; El Salvadorean people; Health behaviour; Drug abuse DO - http://dx.doi.org/10.1080/13557850601002155 ER - TY - JOUR T1 - The Impact of Partner Alcohol Problems on Women's Physical and Mental Health AN - 57089256; 200801803 AB - Objective: The purpose of this study was to examine the association between partner alcohol problems and selected physical and mental health outcomes among married or cohabiting women, before and after adjusting for potential confounders, and to compare these associations with those reflecting the impact of the women's own alcohol-use disorders (AUDs). Method: This analysis is based on data from the Wave 1 200 1-2002 National Epidemiologic Survey on Alcohol and Related Conditions (NESARC), a cross-sectional, retrospective survey of a nationally representative sample of U.S. adults 18 years of age and older. The analytic sample consisted of 11,683 married or cohabiting women. Classification of their own AUDs was based on self-report of symptoms operationalizing the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV), criteria for alcohol abuse or dependence. Current partner alcohol problems were identified by the women after an explanation that recapitulated the essence of these criteria. Physical health measures included criminal victimization of any type, injury, emergency-department and hospital visits, self-reported fair or poor health, and Short Form-12 Health Survey Questionnaire, Version 2 (SF-12v2), -based physical quality of life. Mental health measures included DSM-IV mood and anxiety disorders, number of past-year stressors, and SF-l 2v2-based mental/psychological quality life. All measures refer to the 12 months immediately preceding the interview. Associations were tested using bivariate and multivariate 101 and linear regression models. Results: At the bivariate level, which whose partners had alcohol problems were more likely to experience victimization, injury, mood disorders, anxiety disorders, and being fair or poor health than women whose partners did not have alcohol problems (odds ratio [OR]: 1.7-4.5). They also experienced more stressors and had lower mental/psychological quality-of-life scores but one of these differences remained significant after adjusting for potential confounders, which included the significantly greater rates of stance use and AUDs among women whose partners had alcohol problems. Although the magnitudes of the ORs decreased after adjustment (adjusted OR [AOR]: 2.1-3.4), they generally exceeded the A associated with the women's own AUDs. Conclusions: Partner ale problems pose diverse health threats for women that go beyond well-documented association with domestic violence. Mood, an stress, general health, and quality-of-life problems should be address by groups that provide couples' treatment or counseling to female partners of alcoholics. Adapted from the source document. JF - Journal of Studies on Alcohol and Drugs AU - Dawson, Deborah A AU - Grant, Bridget F AU - Chou, S Patricia AU - Stinson, Frederick S AD - Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health NIAAA/LEB Room 3071, 5635 Fishers Lane, MSC 9304, Bethesda, Maryland 20892-9304 ddawson@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 66 EP - 75 PB - Center of Alcohol Studies, Rutgers, The State University of New Jersey, Piscataway VL - 68 IS - 1 SN - 1937-1888, 1937-1888 KW - Partners KW - Alcohol abuse KW - Women KW - Health status KW - Problem drinking KW - Victimization KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57089256?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Studies+on+Alcohol+and+Drugs&rft.atitle=The+Impact+of+Partner+Alcohol+Problems+on+Women%27s+Physical+and+Mental+Health&rft.au=Dawson%2C+Deborah+A%3BGrant%2C+Bridget+F%3BChou%2C+S+Patricia%3BStinson%2C+Frederick+S&rft.aulast=Dawson&rft.aufirst=Deborah&rft.date=2007-01-01&rft.volume=68&rft.issue=1&rft.spage=66&rft.isbn=&rft.btitle=&rft.title=Journal+of+Studies+on+Alcohol+and+Drugs&rft.issn=19371888&rft_id=info:doi/ LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2008-02-04 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Women; Health status; Alcohol abuse; Problem drinking; Partners; Victimization ER - TY - JOUR T1 - Effects of Mild Early Life Stress on Abnormal Emotion-related Behaviors in 5-HTT Knockout Mice AN - 57076006; 200720266 AB - A low-expressing polymorphic variant of the serotonin transporter (5-HTT) gene has been associated with emotional disorders in humans and non-human primates following exposure to early life trauma. 5-HTT gene knockout (KO) mice exhibit increased anxiety- and depression-related behaviors, and provide a model to study interactions between 5-HTT gene variation and early life stress. The present study assessed the effects of postnatal footshock stress on the development of emotion-related behaviors in 5-HTT KO mice. Results showed that 5-HTT KO mice displayed a profile of suppressed exploratory behavior and increased anxiety- like behavior in the light/dark, elevated plus-maze and open field tests, as well as increased depression-related behavior in the forced swim test following repeated exposure to the test. Postnatal exposure to footshock stress did not affect emotion-related behaviors in non- mutant C57BL/6J mice or modify phenotypic abnormalities in 5-HTT KO. Data provide further evidence of emotional abnormalities following genetic disruption of the 5-HTT. Adapted from the source document. JF - Behavior Genetics AU - Carroll, Jenna C AU - Boyce-Rustay, Janel M AU - Millstein, Rachel AU - Yang, Rebecca AU - Wiedholz, Lisa M AU - Murphy, Dennis L AU - Holmes, Andrew AD - Section on Behavioral Science and Genetics, Laboratory for Integrative Neuroscience, National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Bethesda, Rockville, MD 20852, USA E-mail: holmesan@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 214 EP - 222 PB - Springer Science+Business Media, Inc, New York, NY VL - 37 IS - 1 SN - 0001-8244, 0001-8244 KW - 5-HT, Serotonin transporter, Anxiety, Depression Gene, Mouse KW - Genes KW - Depression KW - Anxiety KW - Laboratory research KW - Serotonin KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/57076006?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Behavior+Genetics&rft.atitle=Effects+of+Mild+Early+Life+Stress+on+Abnormal+Emotion-related+Behaviors+in+5-HTT+Knockout+Mice&rft.au=Carroll%2C+Jenna+C%3BBoyce-Rustay%2C+Janel+M%3BMillstein%2C+Rachel%3BYang%2C+Rebecca%3BWiedholz%2C+Lisa+M%3BMurphy%2C+Dennis+L%3BHolmes%2C+Andrew&rft.aulast=Carroll&rft.aufirst=Jenna&rft.date=2007-01-01&rft.volume=37&rft.issue=1&rft.spage=214&rft.isbn=&rft.btitle=&rft.title=Behavior+Genetics&rft.issn=00018244&rft_id=info:doi/10.1007%2Fs10519-006-9129-9 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2007-12-10 N1 - Last updated - 2016-09-27 N1 - CODEN - BHGHAT N1 - SubjectsTermNotLitGenreText - Anxiety; Depression; Laboratory research; Genes; Serotonin DO - http://dx.doi.org/10.1007/s10519-006-9129-9 ER - TY - JOUR T1 - Associations of perceived risk and worry with cancer health-protective actions: data from the Health Information National Trends Survey (HINTS) AN - 36664911; 3420634 AB - This study examined the associations of susceptibility, conceptualized as both a cognition (risk) and as affect (worry) and their possible interaction, with cancer screening behaviors. Data for this study were obtained from the 2003 Health Information National Trends Survey (HINTS). Hierarchical regression models assessed the ability of risk, worry and their interaction (after controlling for other important variables) to predict cancer-screening behaviors. Results found that risk and worry (but not their interaction) were associated with regular mammography screening and having had a sigmoidoscopy or colonoscopy but with neither FOBT nor PSA screening. The findings suggest that risk and worry are both important in predicting some types of screening behavior and that these variables operate independently. Reprinted by permission of Sage Publications Ltd JF - Journal of health psychology AU - Moser, Richard P AU - McCaul, Kevin AU - Peters, Ellen AU - Nelson, Wendy AU - Marcus, Stephen E AD - National Cancer Institute ; North Dakota State University ; University of Oregon Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 53 EP - 65 VL - 12 IS - 1 SN - 1359-1053, 1359-1053 KW - Sociology KW - Risk KW - Medical sociology KW - Perception KW - Regression analysis KW - Data analysis KW - Cancer KW - Behavioural sciences KW - Health promotion UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36664911?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+health+psychology&rft.atitle=Associations+of+perceived+risk+and+worry+with+cancer+health-protective+actions%3A+data+from+the+Health+Information+National+Trends+Survey+%28HINTS%29&rft.au=Moser%2C+Richard+P%3BMcCaul%2C+Kevin%3BPeters%2C+Ellen%3BNelson%2C+Wendy%3BMarcus%2C+Stephen+E&rft.aulast=Moser&rft.aufirst=Richard&rft.date=2007-01-01&rft.volume=12&rft.issue=1&rft.spage=53&rft.isbn=&rft.btitle=&rft.title=Journal+of+health+psychology&rft.issn=13591053&rft_id=info:doi/10.1177%2F1359105307071735 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 7887 12008; 1542 11325; 3279 971 3286; 10739 12228 10919; 1939 3617 6220; 5790 5772; 9382; 11035 DO - http://dx.doi.org/10.1177/1359105307071735 ER - TY - JOUR T1 - Recent studies on the stages of change model AN - 36662455; 3420748 JF - Journal of health psychology AU - Joekes, Katherine AU - Elderen, Thérèse Van AU - Schreurs, Karlein AU - Mulholland, Ellen AU - Wersch, Anna Van AU - Hanson, Margaret D AU - Chen, Edith AU - Priest, Penny AU - Moser, Richard P AU - McCaul, Kevin AU - Peters, Ellen AU - Nelson, Wendy AU - Marcus, Stephen E AU - Blaine, Bruce E AU - Rodman, Jennifer AU - Newman, Jennifer M AU - Beauchamp, Mark R AU - Welch, Amy S AU - Hulley, Angie J AU - Pakenham, Kenneth I AU - Chiu, Jessica AU - Bursnall, Samantha AU - Cannon, Toni AU - Gooden, Rebecca J AU - Winefield, Helen R AU - Ekelund, Marie-Louise AU - Andersson, Sven Ingmar AU - Lafrance, Michelle N AU - Rutter, Claire L AU - Rutter, Derek R AU - Armitage, Christopher J AU - Arden, Madelynne A AU - Lawrence, Wendy T AU - Prochaska, James O AU - Prochaska, Janice M AU - Marter, Deborah Van AU - Johnson, Janet L AU - Wallace, Louise M AU - Evers, Kerry E AU - Wareing, Hilary AU - Dunn, Orla M AU - Newby, Kate AU - Paiva, Andrea AU - Whyshall, Zara J AU - Haslam, Cheryl AU - Haslam, Roger AD - Leiden University ; Roessingh Rehabilitation Centre, The Netherlands ; University of Teesside ; University of British Columbia ; University of Birmingham ; National Cancer Institute ; North Dakota State University ; University of Oregon ; St John Fisher College ; Hofstra University ; University of Leeds ; University of Queensland ; Griffith University ; Carers Queensland ; University of Adelaide ; Lund University ; St Thomas University ; University of Kent ; University of Sheffield ; Sheffield Hallam University ; University of Southampton ; University of Rhode Island ; Pro-Change Behavior Systems ; Coventry University ; Public Management Associates ; Loughborough University Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 4 EP - 200 VL - 12 IS - 1 SN - 1359-1053, 1359-1053 KW - Sociology KW - Self-evaluation KW - Medical sociology KW - Socioeconomic status KW - Substance use KW - Health services KW - Public health KW - Smoking KW - Behaviourism KW - Attitudes KW - Caring KW - Medical treatment KW - Group dynamics KW - Health promotion KW - Quality of life KW - Obesity KW - Social psychology KW - Mental stress KW - Adolescence KW - Well-being KW - Gender differentiation KW - Patients KW - Stigma KW - United Kingdom KW - Family studies KW - Sex education KW - Prevention KW - Contraception KW - Social support KW - Perception KW - Mental health KW - Occupations KW - Bullying KW - Leadership KW - Work place UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36662455?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+health+psychology&rft.atitle=Recent+studies+on+the+stages+of+change+model&rft.au=Joekes%2C+Katherine%3BElderen%2C+Th%C3%A9r%C3%A8se+Van%3BSchreurs%2C+Karlein%3BMulholland%2C+Ellen%3BWersch%2C+Anna+Van%3BHanson%2C+Margaret+D%3BChen%2C+Edith%3BPriest%2C+Penny%3BMoser%2C+Richard+P%3BMcCaul%2C+Kevin%3BPeters%2C+Ellen%3BNelson%2C+Wendy%3BMarcus%2C+Stephen+E%3BBlaine%2C+Bruce+E%3BRodman%2C+Jennifer%3BNewman%2C+Jennifer+M%3BBeauchamp%2C+Mark+R%3BWelch%2C+Amy+S%3BHulley%2C+Angie+J%3BPakenham%2C+Kenneth+I%3BChiu%2C+Jessica%3BBursnall%2C+Samantha%3BCannon%2C+Toni%3BGooden%2C+Rebecca+J%3BWinefield%2C+Helen+R%3BEkelund%2C+Marie-Louise%3BAndersson%2C+Sven+Ingmar%3BLafrance%2C+Michelle+N%3BRutter%2C+Claire+L%3BRutter%2C+Derek+R%3BArmitage%2C+Christopher+J%3BArden%2C+Madelynne+A%3BLawrence%2C+Wendy+T%3BProchaska%2C+James+O%3BProchaska%2C+Janice+M%3BMarter%2C+Deborah+Van%3BJohnson%2C+Janet+L%3BWallace%2C+Louise+M%3BEvers%2C+Kerry+E%3BWareing%2C+Hilary%3BDunn%2C+Orla+M%3BNewby%2C+Kate%3BPaiva%2C+Andrea%3BWhyshall%2C+Zara+J%3BHaslam%2C+Cheryl%3BHaslam%2C+Roger&rft.aulast=Joekes&rft.aufirst=Katherine&rft.date=2007-01-01&rft.volume=12&rft.issue=1&rft.spage=4&rft.isbn=&rft.btitle=&rft.title=Journal+of+health+psychology&rft.issn=13591053&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - SuppNotes - Collection of 18 articles N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 11474 4551; 13530 13521; 10525 12162 3898; 9271 7890 5792 10484; 7887 12008; 5792 10484; 12258 11762 11859 11856; 1378 10404; 11988 4011 3974 9390 11932 2328 11935 5837 2360 2688 2449 10404 11936; 12357; 590 652 5676 646 6091; 4783; 11901 10404; 5648 5636 5676 971; 7947 5772 7954; 9382; 5790 5772; 8823; 7890 5792 10484; 7271 1411; 1543; 7953 7954; 2039 13521; 8864; 11938 11949 13521; 5423 3549 2688 2449 10404; 1829 6823; 11755 5707 6071 1542 11325; 10072; 11548 4049; 2836 1636 1635 11574; 10449 5772; 13673 4214; 438 462 129 302 ER - TY - JOUR T1 - Mixture cure survival models with dependent censoring AN - 36660345; 3428121 AB - The paper is motivated by cure detection among the prostate cancer patients in the National Institutes of Health surveillance epidemiology and end results programme, wherein the main end point (e.g. deaths from prostate cancer) and the censoring causes (e.g. deaths from heart diseases) may be dependent. Although many researchers have studied the mixture survival model to analyse survival data with non-negligible cure fractions, none has studied the mixture cure model in the presence of dependent censoring. To account for such dependence, we propose a more general cure model that allows for dependent censoring. We derive the cure models from the perspective of competing risks and model the dependence between the censoring time and the survival time by using a class of Archimedean copula models. Within this framework, we consider the parameter estimation, the cure detection and the two-sample comparison of latency distributions in the presence of dependent censoring when a proportion of patients is deemed cured. Large sample results by using martingale theory are obtained. We examine the finite sample performance of the proposed methods via simulation and apply them to analyse the surveillance epidemiology and end results prostate cancer data. Reprinted by permission of Blackwell Publishers JF - Journal of the Royal Statistical Society AU - Li, Y. AU - Tiwari, R C AU - Guha, S AD - Harvard University ; National Cancer Institute, Bethesda Y1 - 2007 PY - 2007 DA - 2007 SP - 285 EP - 306 VL - 69 IS - 3 SN - 1369-7412, 1369-7412 KW - Sociology KW - Health KW - Patients KW - Censuses KW - Statistical methods KW - Cancer UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36660345?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Royal+Statistical+Society&rft.atitle=Mixture+cure+survival+models+with+dependent+censoring&rft.au=Li%2C+Y.%3BTiwari%2C+R+C%3BGuha%2C+S&rft.aulast=Li&rft.aufirst=Y.&rft.date=2007-01-01&rft.volume=69&rft.issue=3&rft.spage=285&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Royal+Statistical+Society&rft.issn=13697412&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 1939 3617 6220; 9271 7890 5792 10484; 5772; 2105 12429; 12228 10919 ER - TY - JOUR T1 - Estimating efficacy in a proposed randomized trial with initial and later non-compliance AN - 36613702; 3397346 AB - A controversial topic in obstetrics is the effect of walking on the probability of Caesarean section among women in labour. A major reason for the controversy is the presence of non-compliance that complicates the estimation of efficacy, the effect of treatment received on outcome. The intent-to-treat method does not estimate efficacy, and estimates of efficacy that are based directly on treatment received may be biased because they are not protected by randomization. However, when non-compliance occurs immediately after randomization, the use of a potential outcomes model with reasonable assumptions has made it possible to estimate efficacy and still to retain the benefits of randomization to avoid selection bias. In this obstetrics application, non-compliance occurs initially and later in one arm. Consequently some parameters cannot be uniquely estimated without making strong assumptions. This difficulty is circumvented by a new study design involving an additional randomization group and a novel potential outcomes model (principal stratification). Reprinted by permission of Blackwell Publishers JF - Journal of the Royal Statistical Society AU - Baker, S G AU - Frangakis, C AU - Lindeman, K S AD - National Institutes of Health, Bethesda ; Johns Hopkins University, Baltimore ; Johns Hopkins Medical Institution, Baltimore Y1 - 2007 PY - 2007 DA - 2007 SP - 211 EP - 222 VL - 56 IS - 2 SN - 0035-9254, 0035-9254 KW - Sociology KW - Medical research KW - Women KW - Statistical models KW - Estimation KW - Statistical methods KW - Pregnancy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36613702?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Royal+Statistical+Society&rft.atitle=Estimating+efficacy+in+a+proposed+randomized+trial+with+initial+and+later+non-compliance&rft.au=Baker%2C+S+G%3BFrangakis%2C+C%3BLindeman%2C+K+S&rft.aulast=Baker&rft.aufirst=S&rft.date=2007-01-01&rft.volume=56&rft.issue=2&rft.spage=211&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Royal+Statistical+Society&rft.issn=00359254&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 12228 10919; 12230 8163; 7886 10902; 10020; 13598 5421 6091; 4403 7854 ER - TY - JOUR T1 - Maximum likelihood inference on a mixed conditionally and marginally specified regression model for genetic epidemiologic studies with two-phase sampling AN - 36599363; 3385594 AB - Two-phase stratified sampling designs can reduce the cost of genetic epidemiologic studies by limiting expensive ascertainments of genetic and environmental exposure to an efficiently selected subsample (phase II) of the main study (phase I). Family history and some covariate information, which may be cheaply gathered for all subjects at phase I, can be used for sampling of informative subjects at phase II. We develop alternative maximum likelihood methods for analysis of data from such studies by using a novel regression model that permits the estimation of `marginal' risk parameters that are associated with the genetic and environmental covariates of interest, while simultaneously characterizing the `conditional' risk of the disease associated with family history after adjusting for the other covariates. The methods and appropriate asymptotic theories are developed with and without an assumption of gene-environment independence, allowing the distribution of the environmental factors to remain non-parametric. The performance of the alternative methods and of sampling strategies is studied by using simulated data involving rare and common genetic variants. An application of the methods proposed is illustrated by using a case-control study of colorectal adenoma embedded within the prostate, lung, colorectal and ovarian cancer screening trial. Reprinted by permission of Blackwell Publishers JF - Journal of the Royal Statistical Society AU - Chatterjee, N AU - Chen, Y H AD - National Cancer Institute ; Academia Sinica Y1 - 2007 PY - 2007 DA - 2007 SP - 123 EP - 142 VL - 69 IS - 2 SN - 1369-7412, 1369-7412 KW - Economics KW - Environment KW - Data collection KW - Human genetics KW - Epidemiology KW - Family history KW - Regression analysis KW - Health KW - Statistical methods KW - Data analysis KW - Cancer UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36599363?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Royal+Statistical+Society&rft.atitle=Maximum+likelihood+inference+on+a+mixed+conditionally+and+marginally+specified+regression+model+for+genetic+epidemiologic+studies+with+two-phase+sampling&rft.au=Chatterjee%2C+N%3BChen%2C+Y+H&rft.aulast=Chatterjee&rft.aufirst=N&rft.date=2007-01-01&rft.volume=69&rft.issue=2&rft.spage=123&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Royal+Statistical+Society&rft.issn=13697412&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 4357 7894; 6081 5460 1615 8573 11325; 4309; 3286; 3279 971 3286; 12228 10919; 10739 12228 10919; 1939 3617 6220; 4767 5889; 5772 ER - TY - JOUR T1 - The insignificance of choice and Wallace's normative approach to responsibility AN - 36595902; 3388844 JF - Law and philosophy AU - Litton, P AD - National Institutes of Health Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 67 EP - 93 VL - 26 IS - 1 SN - 0167-5249, 0167-5249 KW - Political Science KW - Responsibility KW - Ethics KW - Law KW - Philosophy KW - Philosophy of law UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36595902?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Law+and+philosophy&rft.atitle=The+insignificance+of+choice+and+Wallace%27s+normative+approach+to+responsibility&rft.au=Litton%2C+P&rft.aulast=Litton&rft.aufirst=P&rft.date=2007-01-01&rft.volume=26&rft.issue=1&rft.spage=67&rft.isbn=&rft.btitle=&rft.title=Law+and+philosophy&rft.issn=01675249&rft_id=info:doi/10.1007%2Fs10982-006-0001-0 LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 10970; 9490 7253 9486; 4408 8282 8281 6085; 7253; 9486 DO - http://dx.doi.org/10.1007/s10982-006-0001-0 ER - TY - JOUR T1 - Empirical-likelihood-based inference in missing response problems and its application in observational studies AN - 36568568; 3371071 AB - The problem of missing response data is ubiquitous in medical and social science studies. In the case of responses that are missing at random (depending on some covariate information), analyses focused only on the complete data may lead to biased results. Various debias methods have been extensively studied in the literature, particularly the weighting method that was motivated by Horvitz and Thompson's estimators. To improve efficiency, Robins, Rotnitzky and Zhao proposed augmented estimating equations based on corrected complete-case analyses. A nice feature of the augmented method is its `double robustness', i.e. the estimator that is derived from the augmented method is asymptotically unbiased if either the underlying missing data mechanism or the underlying regression function is correctly specified. Furthermore, the augmented estimator can achieve full efficiency if both the missing data mechanism and the regression function are correctly specified. In general, however, it is very difficult to specify the regression function correctly, especially when the dimension of covariates is high-this is the so-called curse of dimensionality problem. The augmented estimator has much lower efficiency if the 'working regression model' is not close to the true regression model. In this paper, the empirical likelihood method is employed to seek a constrained empirical likelihood estimation of mean response with the assumption that responses are missing at random. The empirical-likelihood-based estimators enjoy the double-robustness property. Moreover, it is possible that the empirical-likelihood-based inference can produce asymptotically unbiased and efficient estimators even if the true regression function is not completely known. Simulation results indicate that the empirical-likelihood-based estimators are very robust to a misspecification of the propensity score and dominate other competitors in the sense of having smaller mean-square errors. Methods that are developed in this paper have a nice application in observational causal inferences. The propensity score is used to adjust for differences in pretreatment variables in the estimation of average treatment effects. Reprinted by permission of Blackwell Publishers JF - Journal of the Royal Statistical Society AU - Qin, J AU - Zhang, B AD - National Institute of Allergy and Infectious Diseases ; University of Toledo Y1 - 2007 PY - 2007 DA - 2007 SP - 101 EP - 122 VL - 69 IS - 1 SN - 1369-7412, 1369-7412 KW - Sociology KW - Statistics KW - Research methods KW - Empirical research KW - Statistical methods KW - Methodology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/36568568?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aibss&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+Royal+Statistical+Society&rft.atitle=Empirical-likelihood-based+inference+in+missing+response+problems+and+its+application+in+observational+studies&rft.au=Qin%2C+J%3BZhang%2C+B&rft.aulast=Qin&rft.aufirst=J&rft.date=2007-01-01&rft.volume=69&rft.issue=1&rft.spage=101&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+Royal+Statistical+Society&rft.issn=13697412&rft_id=info:doi/ LA - English DB - International Bibliography of the Social Sciences (IBSS) N1 - Date revised - 2013-06-12 N1 - Last updated - 2013-09-16 N1 - SubjectsTermNotLitGenreText - 4200 10902; 7994; 10919; 12233; 12228 10919 ER - TY - JOUR T1 - Claudin proteins in ovarian cancer AN - 21352628; 8720038 AB - Members of the claudin family of tight junction proteins have been found altered in several malignancies, including ovarian cancer. Because claudin-3 and -4 are elevated in the vast majority of ovarian tumors, they may represent useful biomarkers for detection and prognosis, as well as ideal targets for therapy using the Clostridium perfringens enterotoxin. JF - Disease Markers AU - Morin, Patrice J AD - Laboratory of Cellular and Molecular Biology, National Institute on Aging, NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA. Tel.: +1 410 558 8506; Fax: +1 410 558 8386; E-mail: Morinp[at]grc.nia.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 453 EP - 457 PB - IOS Press, Nieuwe Hemweg 6B VL - 23 IS - 5,6 SN - 0278-0240, 0278-0240 KW - Microbiology Abstracts B: Bacteriology KW - Ovarian cancer KW - Malignancy KW - Tight junctions KW - Clostridium perfringens KW - Prognosis KW - Enterotoxins KW - Tumors KW - biomarkers KW - J 02330:Biochemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21352628?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Disease+Markers&rft.atitle=Claudin+proteins+in+ovarian+cancer&rft.au=Morin%2C+Patrice+J&rft.aulast=Morin&rft.aufirst=Patrice&rft.date=2007-01-01&rft.volume=23&rft.issue=5%2C6&rft.spage=453&rft.isbn=&rft.btitle=&rft.title=Disease+Markers&rft.issn=02780240&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-02-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Ovarian cancer; Malignancy; Tight junctions; Prognosis; Enterotoxins; Tumors; biomarkers; Clostridium perfringens ER - TY - JOUR T1 - Role of Interleukin 10 during Persistent Infection with the Relapsing Fever Spirochete Borrelia turicatae AN - 21321792; 11664942 AB - Relapsing fever is an infection characterized by peaks of spirochetemia attributable to antibody selection against variable serotypes. In the absence of B cells, serotypes cannot be cleared, resulting in persistent infection. We previously identified differences in spirochetemia and disease severity during persistent infection of severe combined immunodeficiency mice with isogenic serotypes 1 (Bt1) or 2 (Bt2) of Borrelia turicatae. To investigate this further, we studied pathogen load, clinical disease, cytokine/chemokine production, and inflammation in mice deficient in B (Igh6 super(-/-)) or B and T (Rag1 super(-/-)) cells persistently infected with Bt1 or Bt2. The results showed that Igh6 super(-/-) mice, despite lower spirochetemia, had a significantly aggravated disease course compared with Rag1 super(-/-) mice. Measurement of cytokines revealed a significant positive correlation between pathogen load and interleukin (IL)-10 in blood, brain, and heart. Bt2-infected Rag1 super(-/-) mice harbored the highest spirochetemia and, at the same time, displayed the highest IL-10 plasma levels. In the brain, Bt1, which was five times more neurotropic than Bt2, caused higher IL-10 production. Activated microglia were the main source of IL-10 in brain. IL-10 injected systemically reduced disease and spirochetemia. The results suggest IL-10 plays a protective role as a down-regulator of inflammation and pathogen load during infection with relapsing fever spirochetes. JF - American Journal of Pathology AU - Gelderblom, H AU - Schmidt, J AU - Londono, D AU - Bai, Y AU - Quandt, J AU - Hornung, R AU - Marques, A AU - Martin, R AU - Cadavid, D AD - Cellular Immunology Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 251 EP - 262 VL - 170 IS - 1 SN - 0002-9440, 0002-9440 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Heart KW - Chemokines KW - Serotypes KW - Lymphocytes B KW - Relapsing fever KW - Brain KW - Pathogens KW - Microglia KW - Borrelia turicatae KW - Persistent infection KW - Interleukin 10 KW - Inflammation KW - Blood KW - Spirochetes KW - Antibodies KW - Plasma levels KW - Cytokines KW - Severe combined immunodeficiency KW - J 02350:Immunology KW - F 06910:Microorganisms & Parasites UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21321792?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Pathology&rft.atitle=Role+of+Interleukin+10+during+Persistent+Infection+with+the+Relapsing+Fever+Spirochete+Borrelia+turicatae&rft.au=Gelderblom%2C+H%3BSchmidt%2C+J%3BLondono%2C+D%3BBai%2C+Y%3BQuandt%2C+J%3BHornung%2C+R%3BMarques%2C+A%3BMartin%2C+R%3BCadavid%2C+D&rft.aulast=Gelderblom&rft.aufirst=H&rft.date=2007-01-01&rft.volume=170&rft.issue=1&rft.spage=251&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Pathology&rft.issn=00029440&rft_id=info:doi/10.2353%2Fajpath.2007.060407 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-01-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Heart; Chemokines; Serotypes; Lymphocytes B; Relapsing fever; Brain; Pathogens; Microglia; Interleukin 10; Persistent infection; Inflammation; Spirochetes; Blood; Plasma levels; Antibodies; Cytokines; Severe combined immunodeficiency; Borrelia turicatae DO - http://dx.doi.org/10.2353/ajpath.2007.060407 ER - TY - JOUR T1 - Press Release: Study Identifies Common Flaws in Oncology Microarray Studies AN - 21290317; 7253596 JF - Journal of the National Cancer Institute AU - Widener, Andrea AD - 301-841-1287 , Journal of the National Cancer Institute, jncimedia@oxfordjournals.org Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 97 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 99 IS - 2 SN - 0027-8874, 0027-8874 KW - Biotechnology and Bioengineering Abstracts KW - Oncology KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21290317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Press+Release%3A+Study+Identifies+Common+Flaws+in+Oncology+Microarray+Studies&rft.au=Widener%2C+Andrea&rft.aulast=Widener&rft.aufirst=Andrea&rft.date=2007-01-01&rft.volume=99&rft.issue=2&rft.spage=97&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2010-03-01 N1 - Last updated - 2015-03-31 N1 - SubjectsTermNotLitGenreText - Oncology ER - TY - JOUR T1 - Value Added by Data Sharing: Long-Term Potentiation of Neuroscience Research AN - 21220725; 11280723 AB - Abstract not available. JF - Neuroinformatics AU - Liu, Yuan AU - Ascoli, Giorgio A AD - NINDS, NIH, Bethesda, MD, USA, liuyuan@ninds.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 143 EP - 145 PB - Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA VL - 5 IS - 3 SN - 1539-2791, 1539-2791 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Nervous system KW - Data processing KW - J1T KW - Long-term potentiation KW - Bioinformatics KW - J1L KW - N3 11029:Neurophysiology & biophysics KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21220725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Neuroinformatics&rft.atitle=Value+Added+by+Data+Sharing%3A+Long-Term+Potentiation+of+Neuroscience+Research&rft.au=Liu%2C+Yuan%3BAscoli%2C+Giorgio+A&rft.aulast=Liu&rft.aufirst=Yuan&rft.date=2007-01-01&rft.volume=5&rft.issue=3&rft.spage=143&rft.isbn=&rft.btitle=&rft.title=Neuroinformatics&rft.issn=15392791&rft_id=info:doi/10.1007%2Fs12021-007-0009-0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-12-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - J1T; J1L; Long-term potentiation; Data processing; Nervous system; Bioinformatics DO - http://dx.doi.org/10.1007/s12021-007-0009-0 ER - TY - JOUR T1 - The biologic basis of in vivo angiogenesis imaging AN - 21161797; 11275924 AB - Knowledge of the different physiology and endothelial markers present in tumor vessels is essential to enable both the development of new anti-angiogenic chemotherapeutic agents and of more specific imaging techniques. Tumor blood vessels are disorganized, irregular in caliber, tortuous, and do not have specialized features of normal arterioles, capillaries or venules. Neo-angiogenic tumor vessels have large gaps between or through cells, loose pericytes, and discontinuities or redundant layers within the basement membrane, rendering these vessels hyper-permeable. Furthermore, the endothelia of tumor vessels may express unique markers on their surface. Imaging is becoming increasingly important in the evaluation of angiogenesis. Clinical imaging is minimally invasive and enables sampling of the whole tumor in a nondestructive manner. The patterns of increased permeability seen on Dynamic contrast-enhanced Magnetic Resonance Imaging (DCE-MRI) mirror the known ultrastructural defects associated with angiogenic vessels. Conventional low-molecular weight contrast agents are currently in clinical use for DCE-MRI studies and have proven successful in detecting changes related to novel angiogenic inhibitors. However, they are relatively nonspecific. Macromolecular contrast media may be more suitable for imaging tumor vessels. It is hoped that imaging modalities can be adapted to specifically target markers expressed on the endothelium of tumor vessels. The number of cell surface markers of angiogenesis is relatively low, and only small amounts of contrast agents can bind to these receptors; currently only Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT) tracers have sufficient sensitivity to allow detection at this low level. Despite limitations in their spatial resolution, PET and SPECT imaging are more likely to enter the clinic as targeted angiogenesis imaging methods. The quest for selective targets on the tumor vasculature continues, currently the integrin family of receptors offer the most promise but other targets are being pursued by investigators. Serial analysis of gene expression or in vivo phage display may help identify new, more selective, markers that can be utilized for the targeted imaging and treatment of angiogenesis. JF - Frontiers in Bioscience AU - Ocak, I AU - Baluk, P AU - Barrett, T AU - McDonald, D M AU - Choyke, P AD - Molecular Imaging Program, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2007 PY - 2007 DA - 2007 SP - 3601 EP - 3616 VL - 12 SN - 1093-9946, 1093-9946 KW - Biotechnology and Bioengineering Abstracts KW - Cell surface KW - Macromolecules KW - Serial analysis of gene expression KW - Chemotherapy KW - Phage display KW - Magnetic resonance imaging KW - Angiogenesis KW - pericytes KW - spatial discrimination KW - Tumors KW - Capillaries KW - Single photon emission computed tomography KW - Tracers KW - Permeability KW - Blood vessels KW - Basement membranes KW - Integrins KW - Endothelium KW - Positron emission tomography KW - Contrast media KW - Sampling KW - Arterioles KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21161797?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Frontiers+in+Bioscience&rft.atitle=The+biologic+basis+of+in+vivo+angiogenesis+imaging&rft.au=Ocak%2C+I%3BBaluk%2C+P%3BBarrett%2C+T%3BMcDonald%2C+D+M%3BChoyke%2C+P&rft.aulast=Ocak&rft.aufirst=I&rft.date=2007-01-01&rft.volume=12&rft.issue=&rft.spage=3601&rft.isbn=&rft.btitle=&rft.title=Frontiers+in+Bioscience&rft.issn=10939946&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-12-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Tumors; Angiogenesis; Single photon emission computed tomography; Contrast media; Positron emission tomography; Macromolecules; Sampling; Chemotherapy; Serial analysis of gene expression; Magnetic resonance imaging; spatial discrimination; Endothelium; Permeability; Basement membranes; Blood vessels; Phage display; Capillaries; Arterioles; Tracers; Cell surface; Integrins; pericytes ER - TY - JOUR T1 - Neural network models of tinnitus AN - 21061948; 8581736 AB - In this chapter we review the relatively recent effort on the part of neuroscientists to use computational neural network modeling to investigate the neural basis of subjective tinnitus. There are advantages and challenges in using a modeling framework to understand this complex auditory disorder. The foremost challenge to modeling a subjective condition such as tinnitus is the evaluation of the occurrence of tinnitus in the model. We propose comparing measures of the model's activities (simulated neuronal activity, behavioral activity, or neuroimaging activity) with experimental data obtained from studies of tinnitus in humans and animals; strong agreement with experimental data will provide support for the validity of the simulation of tinnitus in a particular model. A major advantage of neural network modeling is that it allows experimentation not possible in animals. Models make it possible to evaluate the contribution of different neural mechanisms affecting tinnitus in a principled manner. A model makes predictions that can be tested by experiments thus leading to the designing of focused experiments. We review several neural models of tinnitus and discuss published findings from simulations using these models. We conclude with a proposed scheme for investigating tinnitus that combines neural network modeling with brain imaging experiments. JF - Progress in Brain Research AU - Husain, Fatima T AD - Brain Imaging and Modeling Section, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bldg. 10, Rm 8S235D, MSC 1407, 9000 Rockville Pike, Bethesda, MD 20892, USA, husainf@nidcd.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 125 EP - 140 PB - Elsevier Science, P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl] VL - 166 SN - 0079-6123, 0079-6123 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - neuroimaging KW - electrophysiological KW - fMRI KW - auditory KW - cerebral cortex KW - thalamus KW - Neuroimaging KW - Data processing KW - Neural networks KW - Reviews KW - Animal models KW - Tinnitus KW - Computational neuroscience KW - N3 11002:Computational & theoretical neuroscience KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21061948?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Progress+in+Brain+Research&rft.atitle=Neural+network+models+of+tinnitus&rft.au=Husain%2C+Fatima+T&rft.aulast=Husain&rft.aufirst=Fatima&rft.date=2007-01-01&rft.volume=166&rft.issue=&rft.spage=125&rft.isbn=&rft.btitle=&rft.title=Progress+in+Brain+Research&rft.issn=00796123&rft_id=info:doi/10.1016%2FS0079-6123%2807%2966011-7 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-11-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Neuroimaging; Data processing; Neural networks; Reviews; Animal models; Tinnitus; Computational neuroscience DO - http://dx.doi.org/10.1016/S0079-6123(07)66011-7 ER - TY - JOUR T1 - Production of adenoviral vectors and its recovery AN - 21059914; 8566295 AB - The current demands for adenoviral vectors are increasing to satisfy pre- clinical and clinical gene therapy protocols. Consequently, there is a necessity of methodologies to improve production and recovery of intact particles with the minimum effect upon bioactivity. The production of adenoviral vectors in HEK 293 cells and the potential of an alternative aqueous two-phase system (ATPS) composed of PEG 300-phosphate in recovery of adenoviral vectors were investigated. The production of adenoviral vectors was carried out using a 2 L bioreactor equipped with two Rushton impellers. Different parameters including initial cell density, harvesting time and the addition of a buffer (HEPES) were studied in order to improve the production of adenoviral vectors in HEK 293 cells. A yield of 8 x 10 super(11) infective particles was achieved under the conditions characterized by the addition of Pluronic F-68, inoculation at an initial cell density of 3.5 x 10 super(5) cells/mL and harvest of infected cells at 48 h post infection (hpi). This material was used for the evaluation of the ATPS recovery processes. It was demonstrated that the chemical components of the ATPS did not have a significant effect upon the infectivity of the adenoviral vectors and a total recovery of approximately 90% was obtained. These findings contribute to the process development for the manufacture of adenoviral vectors and other nanoparticulate bioproducts. JF - Process Biochemistry AU - Negrete, Alejandro AU - Ling, Tau Chuan AU - Lyddiatt, Andrew AD - Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK, negretea@nhlbi.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1107 EP - 1113 PB - Elsevier Science, The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 42 IS - 7 SN - 1359-5113, 1359-5113 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts KW - Adenoviral vectors KW - HEK 293 KW - ATPS KW - Yield KW - Recovery KW - Expression vectors KW - Infectivity KW - Gene therapy KW - Bioreactors KW - Cell density KW - Inoculation KW - ATP KW - Infection KW - nanoparticles KW - Polyethylene glycol KW - W 30905:Medical Applications KW - V 22310:Genetics, Taxonomy & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21059914?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Process+Biochemistry&rft.atitle=Production+of+adenoviral+vectors+and+its+recovery&rft.au=Negrete%2C+Alejandro%3BLing%2C+Tau+Chuan%3BLyddiatt%2C+Andrew&rft.aulast=Negrete&rft.aufirst=Alejandro&rft.date=2007-01-01&rft.volume=42&rft.issue=7&rft.spage=1107&rft.isbn=&rft.btitle=&rft.title=Process+Biochemistry&rft.issn=13595113&rft_id=info:doi/10.1016%2Fj.procbio.2007.05.004 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-11-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Expression vectors; Infectivity; Gene therapy; Bioreactors; Cell density; Inoculation; ATP; Infection; Polyethylene glycol; nanoparticles DO - http://dx.doi.org/10.1016/j.procbio.2007.05.004 ER - TY - JOUR T1 - Discovering functional linkages and uncharacterized cellular pathways using phylogenetic profile comparisons: a comprehensive assessment AN - 21001080; 9022919 AB - Background A widely-used approach for discovering functional and physical interactions among proteins involves phylogenetic profile comparisons (PPCs). Here, proteins with similar profiles are inferred to be functionally related under the assumption that proteins involved in the same metabolic pathway or cellular system are likely to have been co-inherited during evolution. Results Our experimentation with E. coli and yeast proteins with 16 different carefully composed reference sets of genomes revealed that the phyletic patterns of proteins in prokaryotes alone could be adequate enough to make reasonably accurate functional linkage predictions. A slight improvement in performance is observed on adding few eukaryotes into the reference set, but a noticeable drop-off in performance is observed with increased number of eukaryotes. Inclusion of most parasitic, pathogenic or vertebrate genomes and multiple strains of the same species into the reference set do not necessarily contribute to an improved sensitivity or accuracy. Interestingly, we also found that evolutionary histories of individual pathways have a significant affect on the performance of the PPC approach with respect to a particular reference set. For example, to accurately predict functional links in carbohydrate or lipid metabolism, a reference set solely composed of prokaryotic (or bacterial) genomes performed among the best compared to one composed of genomes from all three super-kingdoms; this is in contrast to predicting functional links in translation for which a reference set composed of prokaryotic (or bacterial) genomes performed the worst. We also demonstrate that the widely used random null model to quantify the statistical significance of profile similarity is incomplete, which could result in an increased number of false-positives. Conclusion Contrary to previous proposals, it is not merely the number of genomes but a careful selection of informative genomes in the reference set that influences the prediction accuracy of the PPC approach. We note that the predictive power of the PPC approach, especially in eukaryotes, is heavily influenced by the primary endosymbiosis and subsequent bacterial contributions. The over-representation of parasitic unicellular eukaryotes and vertebrates additionally make eukaryotes less useful in the reference sets. Reference sets composed of highly non-redundant set of genomes from all three super-kingdoms fare better with pathways showing considerable vertical inheritance and strong conservation (e.g. translation apparatus), while reference sets solely composed of prokaryotic genomes fare better for more variable pathways like carbohydrate metabolism. Differential performance of the PPC approach on various pathways, and a weak positive correlation between functional and profile similarities suggest that caution should be exercised while interpreting functional linkages inferred from genome-wide large-scale profile comparisons using a single reference set. JF - BMC Bioinformatics AU - Jothi, Raja AU - Przytycka, Teresa M AU - Aravind, L AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA, jothi@ncbi.nlm.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 173 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology; Biotechnology and Bioengineering Abstracts KW - Phylogeny KW - Genomes KW - Translation KW - Carbohydrate metabolism KW - Statistics KW - Heredity KW - Statistical analysis KW - Lipid metabolism KW - Escherichia coli KW - Metabolic pathways KW - Conservation KW - Prokaryotes KW - Carbohydrates KW - Bioinformatics KW - J 02320:Cell Biology KW - G 07770:Bacteria KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/21001080?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=Discovering+functional+linkages+and+uncharacterized+cellular+pathways+using+phylogenetic+profile+comparisons%3A+a+comprehensive+assessment&rft.au=Jothi%2C+Raja%3BPrzytycka%2C+Teresa+M%3BAravind%2C+L&rft.aulast=Jothi&rft.aufirst=Raja&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=173&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-173 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Genomes; Phylogeny; Translation; Carbohydrate metabolism; Statistics; Heredity; Statistical analysis; Lipid metabolism; Metabolic pathways; Conservation; Bioinformatics; Carbohydrates; Prokaryotes; Escherichia coli DO - http://dx.doi.org/10.1186/1471-2105-8-173 ER - TY - JOUR T1 - Two Distinct Cytotoxic Activities of Subtilase Cytotoxin Produced by Shiga-Toxigenic Escherichia coli AN - 20988776; 7207627 AB - Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produced as His-tagged proteins. SubAB induced vacuolation at concentrations greater than 1 mu g/ml after 8 h, in addition to the reported cytotoxicity induced at a ng/ml level after 48 h. Vacuolation was induced with the B, but not the A, subunit and was dependent on V-type ATPase. The cytotoxicity of SubAB at low concentrations was associated with the inhibition of protein synthesis; the 50% inhibitory dose was similar to 1 ng/ml. The A subunit, containing serine 272, which is thought to be a part of the catalytic triad of a subtilase-like serine protease, plus the B subunit was necessary for this activity, both in vivo and in vitro. SubAB did not cleave azocasein, bovine serum albumin, ovalbumin, or synthetic peptides. These data suggest that SubAB is a unique AB toxin: first, the B subunit alone can induce vacuolation; second, the A subunit containing serine 272 plus the B subunit inhibited protein synthesis, both in vivo and in vitro; and third, the A subunit proteolytic activity may have a strict range of substrate specificity. JF - Infection and Immunity AU - Morinaga, Naoko AU - Yahiro, Kinnosuke AU - Matsuura, Gen AU - Watanabe, Masaharu AU - Nomura, Fumio AU - Moss, Joel AU - Noda, Masatoshi AD - Department of Molecular Infectiology. Department of Pediatric Surgery. Department of Molecular Diagnosis and Clinical Genetics, Graduate School of Medicine. Division of Laboratory Medicine, Chiba University Hospital, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba 260-8670, Japan. Pulmonary-Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-1590 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 488 EP - 496 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 75 IS - 1 SN - 0019-9567, 0019-9567 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology KW - Proteolysis KW - Clinical isolates KW - Cell surface KW - Ovalbumin KW - synthetic peptides KW - Adenosinetriphosphatase KW - Protein biosynthesis KW - Data processing KW - Serine proteinase KW - Cytotoxins KW - subtilase KW - Substrate specificity KW - Azocasein KW - Toxins KW - Cytotoxicity KW - Antibodies KW - Bovine serum albumin KW - Escherichia coli KW - Serine KW - J 02330:Biochemistry KW - F 06910:Microorganisms & Parasites UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20988776?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Two+Distinct+Cytotoxic+Activities+of+Subtilase+Cytotoxin+Produced+by+Shiga-Toxigenic+Escherichia+coli&rft.au=Morinaga%2C+Naoko%3BYahiro%2C+Kinnosuke%3BMatsuura%2C+Gen%3BWatanabe%2C+Masaharu%3BNomura%2C+Fumio%3BMoss%2C+Joel%3BNoda%2C+Masatoshi&rft.aulast=Morinaga&rft.aufirst=Naoko&rft.date=2007-01-01&rft.volume=75&rft.issue=1&rft.spage=488&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Clinical isolates; Proteolysis; Ovalbumin; Cell surface; Data processing; Protein biosynthesis; Adenosinetriphosphatase; synthetic peptides; Serine proteinase; Cytotoxins; subtilase; Substrate specificity; Azocasein; Toxins; Antibodies; Cytotoxicity; Bovine serum albumin; Serine; Escherichia coli ER - TY - JOUR T1 - Variance of estimated DTI-derived parameters via first-order perturbation methods AN - 20860398; 8368364 AB - In typical applications of diffusion tensor imaging (DTI), DT-derived quantities are used to make a diagnostic, therapeutic, or scientific determination. In such cases it is essential to characterize the variability of these tensor-derived quantities. Parametric and empirical methods have been proposed to estimate the variance of the estimated DT, and quantities derived from it. However, the former method cannot be generalized since a parametric distribution cannot be found for all DT-derived quantities. Although powerful empirical methods, such as the bootstrap, are available, they require oversampling of the diffusion-weighted imaging (DWI) data. Statistical perturbation methods represent a hybrid between parametric and empirical approaches, and can overcome the primary limitations of both methods. In this study we used a first-order perturbation method to obtain analytic expressions for the variance of DT-derived quantities, such as the trace, fractional anisotropy (FA), eigenvalues, and eigenvectors, for a given experimental design. We performed Monte Carlo (MC) simulations of DTI experiments to test and validate these formulae, and to determine their range of applicability for different experimental design parameters, including the signal-to-noise ratio (SNR), diffusion gradient sampling scheme, and number of DWI acquisitions. This information should be useful for designing DTI studies and assessing the quality of inferences drawn from them. JF - Magnetic Resonance in Medicine AU - Chang, Lin-Ching AU - Koay, Cheng Guan AU - Pierpaoli, Carlo AU - Basser, Peter J AD - Section on Tissue Biophysics and Biomimetics, Laboratory of Integrative Medicine and Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA, changlin@nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 141 EP - 149 PB - John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/] VL - 57 IS - 1 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Anisotropy KW - Data processing KW - Statistics KW - Hybrids KW - Magnetic resonance imaging KW - Diffusion KW - N.M.R. KW - Sampling KW - imaging KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20860398?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=Variance+of+estimated+DTI-derived+parameters+via+first-order+perturbation+methods&rft.au=Chang%2C+Lin-Ching%3BKoay%2C+Cheng+Guan%3BPierpaoli%2C+Carlo%3BBasser%2C+Peter+J&rft.aulast=Chang&rft.aufirst=Lin-Ching&rft.date=2007-01-01&rft.volume=57&rft.issue=1&rft.spage=141&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.21111 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Statistics; imaging; Sampling; Data processing; N.M.R.; Diffusion; Anisotropy; Magnetic resonance imaging; Hybrids DO - http://dx.doi.org/10.1002/mrm.21111 ER - TY - JOUR T1 - High-resolution 3D arteriography of chronic total peripheral occlusions using a T1-W turbo spin-echo sequence with inner-volume imaging AN - 20860233; 8368354 AB - Percutaneous revascularization of peripheral artery chronic total occlusion (CTO) is challenging under X-ray guidance without direct image feedback, due to poor visualization of the obstructed segment and underappreciation of vessel tortuosity. Operators are required to steer interventional devices relatively blindly, and therefore procedural failure or perforation may occur. Alternatively, MRI may allow complete visualization of both patent and occluded arterial segments. We designed and implemented a 3D high-resolution, T1-weighted (T1-W) turbo spin-echo (TSE) MRI sequence with inner-volume (IV) imaging to enable detailed peripheral artery CTO imaging. Using this sequence, high-resolution volumes of interest (VOIs) around the vessel were achieved within 5-10 min. This imaging approach may be used for rapid pre- and postprocedural evaluations, and as a 3D roadmap that can be overlaid during real-time X-, MR-, or XMR-guided catheterization. Experiments were successfully performed on a carotid CTO model in swine ex vivo, and in peripheral arteries in normal volunteers and patients in vivo. Delineation of the vascular architecture, including contrast differences between the patent and occluded artery segments, and lesion morphology heterogeneity were visualized. JF - Magnetic Resonance in Medicine AU - Sampath, Smita AU - Raval, Amish N AU - Lederman, Robert J AU - McVeigh, Elliot R AD - Laboratory of Cardiac Energetics, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, DHHS, Bethesda, Maryland, USA, sampaths@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 40 EP - 49 PB - John Wiley & Sons, Baffins Lane Chichester W. Sussex PO19 1UD UK, [mailto:customer@wiley.co.uk], [URL:http://www.wiley.com/] VL - 57 IS - 1 SN - 0740-3194, 0740-3194 KW - Biotechnology and Bioengineering Abstracts KW - Catheterization KW - Arteriography KW - Ionizing radiation KW - Occlusion KW - Patents KW - Arteries KW - Magnetic resonance imaging KW - Feedback KW - Vascular system KW - W 30910:Imaging UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20860233?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Magnetic+Resonance+in+Medicine&rft.atitle=High-resolution+3D+arteriography+of+chronic+total+peripheral+occlusions+using+a+T1-W+turbo+spin-echo+sequence+with+inner-volume+imaging&rft.au=Sampath%2C+Smita%3BRaval%2C+Amish+N%3BLederman%2C+Robert+J%3BMcVeigh%2C+Elliot+R&rft.aulast=Sampath&rft.aufirst=Smita&rft.date=2007-01-01&rft.volume=57&rft.issue=1&rft.spage=40&rft.isbn=&rft.btitle=&rft.title=Magnetic+Resonance+in+Medicine&rft.issn=07403194&rft_id=info:doi/10.1002%2Fmrm.21098 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Arteries; Magnetic resonance imaging; Patents; Occlusion; Ionizing radiation; Feedback; Vascular system; Arteriography; Catheterization DO - http://dx.doi.org/10.1002/mrm.21098 ER - TY - JOUR T1 - GST, NAT1, CYP1A1 polymorphisms and risk of esophageal and gastric adenocarcinomas AN - 20739404; 8566939 AB - Background: Polymorphisms in glutathione-S-transferase (GST), N-acetyltransferase (NAT) 1, and CYP1A1 genes have been suggested as susceptibility factors for esophageal and gastric adenocarcinomas, but have not been consistently linked to elevated risks. In a population-based case-control study, we examined risks in relation to polymorphisms of the following genes: GSTP1; GSTM1; GSTT1; NAT1; and CYP1A1. Methods: Histologically confirmed incident cases, ages 30-79, were identified in three US locations. Population controls from the same catchment areas were frequency matched to expected age and sex distributions of esophageal and gastric cardia adenocarcinomas. DNA was extracted from buffy coat for PCR-based assays, with interpretable genotyping results obtained from 209 controls, 67 esophageal adenocarcinomas, 60 gastric cardia adenocarcinomas, and 56 noncardia gastric adenocarcinomas. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated among whites, adjusting for age, sex, and study center. Results: In all histologic subgroups, ORs were somewhat elevated for the GSTP1 Val/Val genotype (versus Ile/Ile), although 95% CIs included 1.00. The respective ORs for esophageal, cardia, and other gastric adenocarcinomas were 1.73 (0.75-4.02), 1.46 (0.57-3.73), and 1.22 (0.48-3.09). No consistent patterns of elevated risk were associated with the null GSTM1 or GSTT1 genotypes, one or two copies of NAT1*10 or *11 alleles, or CYP1A1 Val/Val or Ile/Val genotypes (versus Ile/Ile). Conclusions: Additional research in larger samples is needed to further assess polymorphisms and their interactions with epidemiologic risk factors, particularly for esophageal adenocarcinoma, which has been increasing markedly in incidence. JF - Cancer Detection and Prevention AU - Wideroff, Louise AU - Vaughan, Thomas L AU - Farin, Federico M AU - Gammon, Marilie D AU - Risch, Harvey AU - Stanford, Janet L AU - Chow, Wong-Ho AD - Applied Research Program, Division of Cancer Control and Population Sciences, National Cancer Institute, EPN 4005, 6130 Executive Boulevard, 7344, Bethesda, 20892-7344, USA, Wideroff@nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 233 EP - 236 PB - International Society for Preventive Oncology, University of Massachusetts Medical Center 55 Lake Avenue North, Box 20 Worcester MA 01655 USA, [URL:http://www.cancerprev.org/ISPO/] VL - 31 IS - 3 SN - 0361-090X, 0361-090X KW - Risk Abstracts KW - Polymorphism, genetic KW - Cancer risk KW - Gastrointestinal cancer KW - Adenocarcinoma, esophageal KW - Adenocarcinoma, gastric KW - Epidemiology KW - Age KW - prevention KW - DNA KW - Catchments KW - Genotypes KW - Cancer KW - population control KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20739404?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Detection+and+Prevention&rft.atitle=GST%2C+NAT1%2C+CYP1A1+polymorphisms+and+risk+of+esophageal+and+gastric+adenocarcinomas&rft.au=Wideroff%2C+Louise%3BVaughan%2C+Thomas+L%3BFarin%2C+Federico+M%3BGammon%2C+Marilie+D%3BRisch%2C+Harvey%3BStanford%2C+Janet+L%3BChow%2C+Wong-Ho&rft.aulast=Wideroff&rft.aufirst=Louise&rft.date=2007-01-01&rft.volume=31&rft.issue=3&rft.spage=233&rft.isbn=&rft.btitle=&rft.title=Cancer+Detection+and+Prevention&rft.issn=0361090X&rft_id=info:doi/10.1016%2Fj.cdp.2007.03.004 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-10-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Age; Catchments; DNA; prevention; Genotypes; population control; Cancer DO - http://dx.doi.org/10.1016/j.cdp.2007.03.004 ER - TY - JOUR T1 - Genetic Polymorphisms in Folate Metabolism and the Risk of Stomach Cancer AN - 20725800; 7251313 AB - Folate deficiency has been implicated in the etiology of stomach cancer through abnormal DNA methylation and disrupted DNA synthesis and repair. Enzyme-coding genes involved in folate metabolism are often polymorphic. In a population-based study of 305 cases and 427 controls in Warsaw, Poland, we evaluated the risk of stomach cancer in relation to polymorphisms in folate-metabolizing genes, including MTHFR (Ex5+79C>T and Ex8-62A>C), MTR (Ex26-20A>G), and MTRR (Ex2-64A>G, Ex5+123C>T, Ex15+572C>T, Ex15-405A>T, Ex9-85C>T, Ex15-526G>A, and Ex14+14C>T). Polymorphisms in the MTHFR gene were not associated with stomach cancer risk. No notable effect was found for polymorphisms in MTR or MTRR either, although MTR Ex26-20 A>G and MTRR Ex5+123C>T polymorphisms were associated with a borderline increased risk of stomach cancer (MTR Ex26-20A>G, AG/GG versus AA: odds ratio, 1.35; 95% confidence interval, 0.96-1.90; MTRR Ex5+123C>T, CT/TT versus CC: odds ratio, 1.30; 95% confidence interval, 0.93-1.82). We did not find significant interactions between polymorphisms in MTHFR, MTR, and MTRR genes and dietary folate and alcohol consumption. Our study did not identify strong genetic determinants in the folate metabolism pathway for stomach cancer risk. (Cancer Epidemiol Biomarkers Prev 2007; 16(1):115-21) JF - Cancer Epidemiology, Biomarkers & Prevention AU - Zhang, Fang Fang AU - Terry, Mary Beth AU - Hou, Lifang AU - Chen, Jinbo AU - Lissowska, Jolanta AU - Yeager, Meredith AU - Zatonski, Witold AU - Chanock, Stephen AU - Morabia, Alfredo AU - Chow, Wong-Ho AD - Division of Cancer Epidemiology and Genetics and Core Genotyping Facility, Advanced Technology Center, National Cancer Institute, Gaithersburg, Maryland Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 115 EP - 121 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 16 IS - 1 SN - 1055-9965, 1055-9965 KW - Genetics Abstracts; Risk Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Bioindicators KW - Diets KW - Alcohol KW - DNA biosynthesis KW - Etiology KW - Gene polymorphism KW - Population studies KW - Methylenetetrahydrofolate reductase KW - DNA repair KW - biomarkers KW - Cancer KW - Poland KW - DNA KW - prevention KW - DNA methylation KW - Gastric cancer KW - Folic acid KW - Metabolism KW - Ethanol KW - G 07880:Human Genetics KW - N 14820:DNA Metabolism & Structure KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20725800?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Genetic+Polymorphisms+in+Folate+Metabolism+and+the+Risk+of+Stomach+Cancer&rft.au=Zhang%2C+Fang+Fang%3BTerry%2C+Mary+Beth%3BHou%2C+Lifang%3BChen%2C+Jinbo%3BLissowska%2C+Jolanta%3BYeager%2C+Meredith%3BZatonski%2C+Witold%3BChanock%2C+Stephen%3BMorabia%2C+Alfredo%3BChow%2C+Wong-Ho&rft.aulast=Zhang&rft.aufirst=Fang&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=115&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - DNA biosynthesis; Etiology; Gene polymorphism; DNA methylation; Population studies; Methylenetetrahydrofolate reductase; DNA repair; Folic acid; Gastric cancer; biomarkers; Metabolism; Ethanol; Diets; Bioindicators; Alcohol; prevention; DNA; Cancer; Poland ER - TY - JOUR T1 - Variant in Sex Hormone-Binding Globulin Gene and the Risk of Prostate Cancer AN - 20725416; 7251322 AB - Sex hormones have been implicated in prostate carcinogenesis and are thought to modulate cell proliferation and growth. To investigate the association between polymorphisms in hormone-related genes and prostate cancer risk, we conducted a two-stage, case-control study within the screening arm of the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Using DNA extracted from blood specimens, we initially genotyped 14 single nucleotide polymorphisms in genes involved in hormone regulation or metabolism (AKR1C3, CYP1A1, CYP1B1, CYP3A4, ESR1, GNRH1, HSD173B, HSD3B2, SHBG, and SRD5A2) in 488 prostate cancer cases and 617 matched controls. Heterozygotes at SHBG D356N were found to be associated with an increased risk of prostate cancer compared with the homozygous wild type, particularly among non-Hispanic whites (odds ratio, 1.54; 95% confidence interval, 1.13-2.09; P = 0.006). No significant associations were observed with the other polymorphisms. The SHBG D356N polymorphism, which has potential functional significance, was subsequently genotyped in additional 769 cases and 1,168 controls. Overall, SHBG D356N heterozygotes were found to have an increased risk of prostate cancer among whites (odds ratio, 1.34; 95% confidence interval, 1.10-1.63; P = 0.0007). This study suggests that genetic variation in SHBG may influence prostate cancer susceptibility. (Cancer Epidemiol Biomarkers Prev 2007; 16(1):165-8) JF - Cancer Epidemiology, Biomarkers & Prevention AU - Berndt, Sonja I AU - Chatterjee, Nilanjan AU - Huang, Wen-Yi AU - Chanock, Stephen J AU - Welch, Robert AU - Crawford, EDavid AU - Hayes, Richard B AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, Maryland and University of Colorado, Denver, Colorado Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 165 EP - 168 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 16 IS - 1 SN - 1055-9965, 1055-9965 KW - Genetics Abstracts; Risk Abstracts KW - Gene polymorphism KW - Genetic diversity KW - Hormones KW - Sex hormones KW - ESR1 protein KW - prevention KW - prostate cancer KW - Bioindicators KW - Ovarian cancer KW - ovarian carcinoma KW - genetic diversity KW - Globulins KW - biomarkers KW - Cancer KW - Blood KW - Prostate cancer KW - Lung KW - Single-nucleotide polymorphism KW - Carcinogenesis KW - Heterozygotes KW - DNA KW - Cytochrome P450 KW - Cell proliferation KW - Metabolism KW - G 07880:Human Genetics KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20725416?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=Variant+in+Sex+Hormone-Binding+Globulin+Gene+and+the+Risk+of+Prostate+Cancer&rft.au=Berndt%2C+Sonja+I%3BChatterjee%2C+Nilanjan%3BHuang%2C+Wen-Yi%3BChanock%2C+Stephen+J%3BWelch%2C+Robert%3BCrawford%2C+EDavid%3BHayes%2C+Richard+B&rft.aulast=Berndt&rft.aufirst=Sonja&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=165&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Ovarian cancer; Gene polymorphism; Genetic diversity; Globulins; biomarkers; Hormones; Sex hormones; Blood; Prostate cancer; ESR1 protein; Single-nucleotide polymorphism; Heterozygotes; Carcinogenesis; Cytochrome P450; Cell proliferation; Metabolism; Bioindicators; Lung; ovarian carcinoma; DNA; prevention; genetic diversity; prostate cancer; Cancer ER - TY - JOUR T1 - Quantitative evaluation of bacteria released by bathers in a marine water AN - 20723472; 7171115 AB - Enterococci, a common fecal indicator, and Staphylococcus aureus, a common skin pathogen, can be shed by bathers affecting the quality of recreational waters and resulting in possible human health impacts. Due to limited information available concerning human shedding of these microbes, this study focused on estimating the amounts of enterococci and S. aureus shed by bathers directly off their skin and indirectly via sand adhered to skin. Two sets of experiments were conducted at a marine beach located in Miami-Dade County, Florida. The first study, referred to as the "large pool" study, involved 10 volunteers who immersed their bodies in 4700 L during four 15 min cycles with exposure to beach sand in cycles 3 and 4. The "small pool" study involved 10 volunteers who were exposed to beach sand for 30 min before they individually entered a small tub. After each individual was rinsed with off- shore marine water, sand and rinse water were collected and analyzed for enterococci. Results from the "large pool" study showed that bathers shed concentrations of enterococci and S. aureus on the order of 6x10 super(5) and 6x10 super(6) colony forming units (CFU) per person in the first 15 min exposure period, respectively. Significant reductions in the bacteria shed per bather (50% reductions for S. aureus and 40% for enterococci) were observed in the subsequent bathing cycles. The "small pool" study results indicated that the enterococci contribution from sand adhered to skin was small (about 2% of the total) in comparison with the amount shed directly from the bodies of the volunteers. Results indicated that bathers transport significant amounts of enterococci and S. aureus to the water column, and thus human microbial bathing load should be considered as a non-point source when designing recreational water quality models. JF - Water Research AU - Elmir, Samir M AU - Wright, Mary E AU - Abdelzaher, Amir AU - Solo-Gabriele, Helena M AU - Fleming, Lora E AU - Miller, Gary AU - Rybolowik, Michael AU - Shih, Meng-Ta Peter AU - Pillai, Segaran P AU - Cooper, Jennifer A AU - Quaye, Elesi A AD - NSF-NIEHS Oceans and Human Health Center, University of Miami, Rosenstiel School for Marine and Atmospheric Sciences,1801 NW 9 Avenue, Suite 200 (R-669), Miami, Florida 33136, USA, hmsolo@miami.edu Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 3 EP - 10 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl] VL - 41 IS - 1 SN - 0043-1354, 0043-1354 KW - Microbiology Abstracts B: Bacteriology; Pollution Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; ASFA Marine Biotechnology Abstracts; Microbiology Abstracts A: Industrial & Applied Microbiology; Aqualine Abstracts; Water Resources Abstracts KW - Recreational water KW - Bacteria indicators KW - Enterococci KW - Staphylococcus aureus KW - Bathers KW - Sediments KW - Beach sand KW - Non-point pollution sources KW - Water quality models KW - shores KW - USA, Florida KW - Bathing KW - Pools KW - Shores KW - Water quality KW - Water column KW - Models KW - Public health KW - Evaluation KW - Colonies KW - Sand KW - Exposure KW - Recreational waters KW - ASW, USA, Florida KW - Bacteria KW - Beaches KW - Skin KW - Staphylococcus KW - Pathogens KW - Water pollution KW - Recreation areas KW - Colony-forming cells KW - water column KW - Exposed habitats KW - A 01450:Environmental Pollution & Waste Treatment KW - Q4 27760:Microorganisms KW - P 1000:MARINE POLLUTION KW - AQ 00008:Effects of Pollution KW - SW 3030:Effects of pollution KW - J 02400:Human Diseases KW - Q5 08524:Public health, medicines, dangerous organisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20723472?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aaqualine&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Water+Research&rft.atitle=Quantitative+evaluation+of+bacteria+released+by+bathers+in+a+marine+water&rft.au=Elmir%2C+Samir+M%3BWright%2C+Mary+E%3BAbdelzaher%2C+Amir%3BSolo-Gabriele%2C+Helena+M%3BFleming%2C+Lora+E%3BMiller%2C+Gary%3BRybolowik%2C+Michael%3BShih%2C+Meng-Ta+Peter%3BPillai%2C+Segaran+P%3BCooper%2C+Jennifer+A%3BQuaye%2C+Elesi+A&rft.aulast=Elmir&rft.aufirst=Samir&rft.date=2007-01-01&rft.volume=41&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Water+Research&rft.issn=00431354&rft_id=info:doi/10.1016%2Fj.watres.2006.10.005 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Recreational waters; Bathing; Pathogens; Water pollution; Exposed habitats; Public health; Colonies; Beaches; Skin; Sand; Colony-forming cells; Shores; Water quality; Water column; Models; shores; Recreation areas; water column; Evaluation; Bacteria; Exposure; Staphylococcus; Pools; Staphylococcus aureus; ASW, USA, Florida; USA, Florida DO - http://dx.doi.org/10.1016/j.watres.2006.10.005 ER - TY - JOUR T1 - A Case-Control Investigation of Immune Function Gene Polymorphisms and Risk of Testicular Germ Cell Tumors AN - 20722666; 7251339 AB - There is reason to suspect that testicular germ cell tumor (TGCT) development may be influenced by cytokines, secreted proteins that modulate tumor immune surveillance activity as well as a variety of processes in the testis. To address this hypothesis, we conducted a case-control analysis (508 cases, 608 controls) of 32 putatively functional single-nucleotide polymorphisms (SNP) in 16 immune function genes among non-Hispanic Caucasian participants in the U.S. Servicemen's Testicular Tumor Environmental and Endocrine Determinants Study. The TGFB1 Ex5-73C>T variant was positively associated with TGCT (CT/TT versus CC: odds ratio, 1.73; 95% confidence interval, 1.01-2.95; P sub(trend) = 0.05); additionally, haplotypes of the assessed TGFB1 SNPs (-509C>T, 327C>T, Ex1-282C>G, and Ex5-73C>T) differed in frequency between cases and controls (all TGCT, P 0.07; seminoma, P 0.04; nonseminoma, P 0.11). We also observed excess frequencies among TGCT cases versus controls of LTA 252G (P sub(trend) = 0.08) and of the TNF variants -1042C (P sub(trend) = 0.06), -1036T (P sub(trend) = 0.07), and -238G (P sub(trend) = 0.09). Analyses of haplotypes for LTA-TNF SNPs (LTA -91C>A, LTA 252A>G, TNF -863C>A, TNF -857C>T, TNF -308G>A, and -238G>A) were similarly suggestive of an association with TGCT (P = 0.06) and nonseminoma (P = 0.04), but not seminoma (P = 0.21). Polymorphisms in other genes were found to be associated only with seminoma (IL2) or nonseminoma (IFNGR2 and IL10). However, none of the associations remained noteworthy after applying the false discovery rate method to control for multiple testing. In conclusion, our findings suggest that polymorphisms in TGFB1 and LTA/TNF, and possibly other immune function genes, may influence susceptibility to TGCT. (Cancer Epidemiol Biomarkers Prev 2007; 16(1):77-83) JF - Cancer Epidemiology, Biomarkers & Prevention AU - Purdue, Mark P AU - Sakoda, Lori C AU - Graubard, Barry I AU - Welch, Robert AU - Chanock, Stephen J AU - Sesterhenn, Isabel A AU - Rubertone, Mark V AU - Erickson, RLoren AU - McGlynn, Katherine A AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Department of Health and Human Services, Bethesda, Maryland Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 77 EP - 83 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 16 IS - 1 SN - 1055-9965, 1055-9965 KW - Genetics Abstracts; Risk Abstracts; Immunology Abstracts KW - Testes KW - Interleukin 2 KW - Gene polymorphism KW - Tumor necrosis factor KW - tumors KW - Interleukin 10 KW - Haplotypes KW - Immunosurveillance KW - prevention KW - Cytokines KW - Bioindicators KW - Transforming growth factor- beta 1 KW - Germ cells KW - haplotypes KW - Tumors KW - biomarkers KW - Cancer KW - USA KW - Single-nucleotide polymorphism KW - Proteins KW - Immune response KW - seminoma KW - G 07880:Human Genetics KW - F 06915:Cancer Immunology KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20722666?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.atitle=A+Case-Control+Investigation+of+Immune+Function+Gene+Polymorphisms+and+Risk+of+Testicular+Germ+Cell+Tumors&rft.au=Purdue%2C+Mark+P%3BSakoda%2C+Lori+C%3BGraubard%2C+Barry+I%3BWelch%2C+Robert%3BChanock%2C+Stephen+J%3BSesterhenn%2C+Isabel+A%3BRubertone%2C+Mark+V%3BErickson%2C+RLoren%3BMcGlynn%2C+Katherine+A&rft.aulast=Purdue&rft.aufirst=Mark&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=77&rft.isbn=&rft.btitle=&rft.title=Cancer+Epidemiology%2C+Biomarkers+%26+Prevention&rft.issn=10559965&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Testes; Transforming growth factor- beta 1; Interleukin 2; Tumor necrosis factor; Gene polymorphism; Germ cells; Tumors; biomarkers; Cancer; Interleukin 10; Haplotypes; Single-nucleotide polymorphism; Immunosurveillance; Cytokines; Immune response; seminoma; Bioindicators; prevention; Proteins; tumors; haplotypes; USA ER - TY - JOUR T1 - Simian immunodeficiency virus lentivector corrects human X-linked chronic granulomatous disease in the NOD/SCID mouse xenograft AN - 20703431; 7632486 AB - X-linked chronic granulomatous disease (X-CGD) is a primary immunodeficiency caused by mutations in the phagocyte nicotinamide dinucleotide phosphate oxidase catalytic subunit gp91 super(phox). Gene therapy targeting hematopoietic stem cells (HSCs) can correct CGD, but permanent correction remains a challenge. Lentiviral vectors have become attractive tools for gene transfer, and they may have the potential to transduce very primitive HSCs. We used a self-inactivating RD114/TR-pseudotyped simian immunodeficiency virus (SIVmac)-based vector encoding human gp91 super(phox) for ex vivo transduction of peripheral blood-mobilized stem cells (PBSCs) from patients with X-CGD. In PBSCs from two patients, ex vivo transduction efficiencies of 40.5 and 46% were achieved, and correction of oxidase activity was observed in myeloid cells differentiating in culture. When transduced PBSCs from these patients were transplanted into nonobese diabetic/severe combined immunodeficient mice and compared to normal control, 10.5 and 7.3% of the human myeloid cells in bone marrow developing at 6 weeks from the human xenografts expressed the gp91 super(phox) transgene. Sustained functional correction of oxidase activity was documented in myeloid cells differentiated from engrafted transduced PBSCs. Transgene marking was polyclonal as assessed by vector integration site analysis. These data suggest that RD114/TR SIVmac-based vectors might be suitable for gene therapy of CGD and other hereditary hematologic diseases. JF - Gene Therapy AU - Naumann, N AU - De Ravin, S S AU - Choi, U AU - Moayeri, M AU - Whiting-Theobald, N AU - Linton, G F AU - Ikeda, Y AU - Malech, H L AD - Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA, hmalech@niaid.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1513 EP - 1524 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 14 IS - 21 SN - 0969-7128, 0969-7128 KW - Genetics Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts KW - chronic granulomatous disease KW - lentiviral vector KW - simian immunodeficiency virus KW - Gp91 super(phox) KW - hematopoietic stem cell KW - RD114 KW - Data processing KW - Gene therapy KW - nicotinamide KW - X chromosome KW - Transgenes KW - Bone marrow KW - Immunodeficiency KW - Catalytic subunits KW - Cell culture KW - Myeloid cells KW - Transgenic mice KW - Expression vectors KW - Diabetes mellitus KW - Integration KW - Stem cells KW - Phosphate KW - Phagocytes KW - Severe combined immunodeficiency KW - Xenografts KW - Chronic granulomatous disease KW - Mutation KW - Simian immunodeficiency virus KW - Bone marrow transplantation KW - W 30905:Medical Applications KW - V 22360:AIDS and HIV KW - G 07880:Human Genetics UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20703431?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene+Therapy&rft.atitle=Simian+immunodeficiency+virus+lentivector+corrects+human+X-linked+chronic+granulomatous+disease+in+the+NOD%2FSCID+mouse+xenograft&rft.au=Naumann%2C+N%3BDe+Ravin%2C+S+S%3BChoi%2C+U%3BMoayeri%2C+M%3BWhiting-Theobald%2C+N%3BLinton%2C+G+F%3BIkeda%2C+Y%3BMalech%2C+H+L&rft.aulast=Naumann&rft.aufirst=N&rft.date=2007-01-01&rft.volume=14&rft.issue=21&rft.spage=1513&rft.isbn=&rft.btitle=&rft.title=Gene+Therapy&rft.issn=09697128&rft_id=info:doi/10.1038%2Fsj.gt.3303010 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Data processing; Gene therapy; nicotinamide; Transgenes; X chromosome; Immunodeficiency; Bone marrow; Catalytic subunits; Cell culture; Transgenic mice; Myeloid cells; Diabetes mellitus; Expression vectors; Integration; Stem cells; Phosphate; Phagocytes; Severe combined immunodeficiency; Xenografts; Chronic granulomatous disease; Mutation; Bone marrow transplantation; Simian immunodeficiency virus DO - http://dx.doi.org/10.1038/sj.gt.3303010 ER - TY - JOUR T1 - Development of recombinant adeno-associated virus vectors carrying small interfering RNA (shHec1)-mediated depletion of kinetochore Hec1 protein in tumor cells AN - 20702490; 7441383 AB - Transcript depletion using small interfering RNA (siRNA) technology represents a potentially valuable technique for the treatment of cancer. However, delivering therapeutic quantities of siRNA into solid tumors by chemical transfection is not feasible, whereas viral vectors efficiently transduce many human tumor cell lines. Yet producing sufficient quantities of viral vectors that elicit acute and selective cytotoxicity remains a major obstacle for preclinical and clinical trials. Using the invertebrate Spodoptera frugiperda (Sf9) cell line, we were able to produce high titer stocks of cytotoxic recombinant adeno-associated virus (rAAV) that express short hairpin RNA (shRNA) and that efficiently deplete Hec1 (highly expressed in cancer 1), or Kntc2 (kinetochore-associated protein 2), a kinetochore protein directly involved in kinetochore microtubule interactions, chromosome congression and spindle checkpoint signaling. Depletion of Hec1 protein results in persistent spindle checkpoint activation followed by cell death. Because Hec1 expression and activity are only present in mitotic cells, non-dividing cells were not affected by rAAV treatment. On the basis of the results of screening 56 human tumor cell lines with three different serotype vectors, we used a tumor xenograft model to test the effects in vivo. The effects of the shHec1 vector were evident in sectioned and stained tumors. The experiments with rAAV- shRNA vectors demonstrate the utility of producing vectors in invertebrate cells to obtain sufficient concentrations and quantities for solid tumor therapy. This addresses an important requirement for cancer gene therapy, to produce cytotoxic vectors in sufficient quantities and concentrations to enable quantitative transduction and selective killing of solid tumor cells. JF - Gene Therapy AU - Li, L AU - Yang, L AU - Scudiero, D A AU - Miller, S A AU - Yu, Z-X AU - Stukenberg, P T AU - Shoemaker, R H AU - Kotin, R M AD - Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA, kotinr@nhlbi.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 814 EP - 827 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 14 IS - 10 SN - 0969-7128, 0969-7128 KW - Fall armyworm KW - Virology & AIDS Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts; Entomology Abstracts KW - rAAV KW - Hec1 KW - RNA interference KW - cancer BEV KW - baculovirus expression vector KW - CGNS KW - cerebella granule neurons KW - CNS KW - central nervous system KW - dsRNA KW - double-strand RNA KW - eGFP KW - enhanced green fluorescence protein KW - highly expressed in cancer 1 KW - hrGFP KW - humanized Renilla green fluorescence protein KW - HSPG KW - heparan sulfate proteogylcans KW - kmts KW - kinetochore microtubles KW - Kntc2 KW - kinetochore-associated protein 2 KW - MOI KW - multiplicity of infection (encapsidated vector genome particles per cell) KW - recombinant adeno-associated virus KW - RNAi KW - Sf KW - Spodoptera frugiperda KW - shRNA KW - short hairpin RNA KW - siRNA KW - small interfering RNA KW - Microtubules KW - Serotypes KW - Gene therapy KW - Solid tumors KW - Transcription KW - Tumors KW - Clinical trials KW - Adeno-associated virus KW - Cancer KW - Cell activation KW - Expression vectors KW - Spindles KW - Cytotoxicity KW - Tumor cell lines KW - Chromosomes KW - Cell death KW - Transfection KW - Kinetochores KW - Xenografts KW - Signal transduction KW - W 30925:Genetic Engineering KW - N 14830:RNA KW - Z 05330:Reproduction and Development KW - V 22370:Oncology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20702490?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene+Therapy&rft.atitle=Development+of+recombinant+adeno-associated+virus+vectors+carrying+small+interfering+RNA+%28shHec1%29-mediated+depletion+of+kinetochore+Hec1+protein+in+tumor+cells&rft.au=Li%2C+L%3BYang%2C+L%3BScudiero%2C+D+A%3BMiller%2C+S+A%3BYu%2C+Z-X%3BStukenberg%2C+P+T%3BShoemaker%2C+R+H%3BKotin%2C+R+M&rft.aulast=Li&rft.aufirst=L&rft.date=2007-01-01&rft.volume=14&rft.issue=10&rft.spage=814&rft.isbn=&rft.btitle=&rft.title=Gene+Therapy&rft.issn=09697128&rft_id=info:doi/10.1038%2Fsj.gt.3302933 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Microtubules; Serotypes; Gene therapy; Solid tumors; Transcription; Tumors; Clinical trials; Cancer; Cell activation; Expression vectors; Cell death; Chromosomes; Tumor cell lines; Cytotoxicity; Spindles; siRNA; Kinetochores; Transfection; Xenografts; Signal transduction; Spodoptera frugiperda; Adeno-associated virus DO - http://dx.doi.org/10.1038/sj.gt.3302933 ER - TY - JOUR T1 - Intentional Exposure Studies of Environmental Agents on Human Subjects: Assessing Benefits and Risks 1 AN - 20700329; 10308439 AB - In this article, I assess the benefits and risks of studies that intentionally expose research subjects to environmental agents. I describe these types of studies, identify their benefits and risks, compare them to other research methods that can be used to investigate the relationship between environmental exposures and disease, and discuss some issues related to research design and risk minimization. I argue that the benefits of intentional environmental exposure studies outweigh the risks when 1) the knowledge gained is likely to improve our understanding of the relationship between environmental exposure and disease, 2) this knowledge cannot be obtained by other methods, 3) the experiments are well designed, 4) the subjects will receive some benefits, such as medical evaluations, 5) risks are minimized, and 6) the risks to human subjects are less than those encountered in a typical Phase I drug study. Only in rare circumstances (i.e., when an intentional environmental exposure study is needed to implement an important environmental or public health intervention or regulation) may such studies expose research subjects to risks as high as those encountered in a typical Phase I drug trail. JF - Accountability in Research AU - Resnik, D B AD - National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA Y1 - 2007 PY - 2007 DA - 2007 SP - 35 EP - 55 VL - 14 IS - 1 SN - 0898-9621, 0898-9621 KW - Risk Abstracts KW - risk reduction KW - research methods KW - research design KW - intervention KW - accountability KW - Drugs KW - Public health KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20700329?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Accountability+in+Research&rft.atitle=Intentional+Exposure+Studies+of+Environmental+Agents+on+Human+Subjects%3A+Assessing+Benefits+and+Risks+1&rft.au=Resnik%2C+D+B&rft.aulast=Resnik&rft.aufirst=D&rft.date=2007-01-01&rft.volume=14&rft.issue=1&rft.spage=35&rft.isbn=&rft.btitle=&rft.title=Accountability+in+Research&rft.issn=08989621&rft_id=info:doi/10.1080%2F08989620601122842 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-08-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - risk reduction; research methods; intervention; research design; accountability; Drugs; Public health DO - http://dx.doi.org/10.1080/08989620601122842 ER - TY - JOUR T1 - Identifying Biomarkers from Mass Spectrometry Data with Ordinal Outcome AN - 20667877; 9420473 AB - Summary: In recent years, there has been an increased interest in using protein mass spectroscopy to identify molecular markers that discriminate diseased from healthy individuals. Existing methods are tailored towards classifying observations into nominal categories. Sometimes, however, the outcome of interest may be measured on an ordered scale. Ignoring this natural ordering results in some loss of information. In this paper, we propose a Bayesian model for the analysis of mass spectrometry data with ordered outcome. The method provides a unified approach for identifying relevant markers and predicting class membership. This is accomplished by building a stochastic search variable selection method within an ordinal outcome model. We apply the methodology to mass spectrometry data on ovarian cancer cases and healthy individuals. We also utilize wavelet-based techniques to remove noise from the mass spectra prior to analysis. We identify protein markers associated with being healthy, having low grade ovarian cancer, or being a high grade case. For comparison, we repeated the analysis using conventional classification procedures and found improved predictive accuracy with our method. JF - Cancer Informatics AU - Kwon, Deukwoo AU - Tadesse, Mahlet G AU - Sha, Naijun AU - Pfeiffer, Ruth M AU - Vannucci, Marina AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, U.S.A Y1 - 2007 PY - 2007 DA - 2007 SP - 19 EP - 28 PB - Libertas Academica, PO Box 300-874 VL - 3 SN - 1176-9351, 1176-9351 KW - Biotechnology and Bioengineering Abstracts KW - Markov chain Monte Carlo KW - mass spectrometry KW - ordinal outcome KW - variable selection KW - Ovarian cancer KW - Data processing KW - Mathematical models KW - Informatics KW - Bayesian analysis KW - Stochasticity KW - biomarkers KW - Mass spectroscopy KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20667877?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Informatics&rft.atitle=Identifying+Biomarkers+from+Mass+Spectrometry+Data+with+Ordinal+Outcome&rft.au=Kwon%2C+Deukwoo%3BTadesse%2C+Mahlet+G%3BSha%2C+Naijun%3BPfeiffer%2C+Ruth+M%3BVannucci%2C+Marina&rft.aulast=Kwon&rft.aufirst=Deukwoo&rft.date=2007-01-01&rft.volume=3&rft.issue=&rft.spage=19&rft.isbn=&rft.btitle=&rft.title=Cancer+Informatics&rft.issn=11769351&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Ovarian cancer; Mathematical models; Data processing; Bayesian analysis; Informatics; biomarkers; Stochasticity; Mass spectroscopy ER - TY - JOUR T1 - Analysis of Gene Expression Data Using BRB-Array Tools AN - 20663017; 9420477 AB - BRB-ArrayTools is an integrated software system for the comprehensive analysis of DNA microarray experiments. It was developed by professional biostatisticians experienced in the design and analysis of DNA microarray studies and incorporates methods developed by leading statistical laboratories. The software is designed for use by biomedical scientists who wish to have access to state-of-the-art statistical methods for the analysis of gene expression data and to receive training in the statistical analysis of high dimensional data. The software provides the most extensive set of tools available for predictive classifier development and complete cross-validation. It offers extensive links to genomic websites for gene annotation and analysis tools for pathway analysis. An archive of over 100 datasets of published microarray data with associated clinical data is provided and BRB-ArrayTools automatically imports data from the Gene Expression Omnibus public archive at the National Center for Biotechnology Information. JF - Cancer Informatics AU - Simon, Richard AU - Lam, Amy AU - Li, Ming-Chung AU - Ngan, Michael AU - Menenzes, Supriya AU - Zhao, Yingdong AD - Biometric Research Branch, National Cancer Institute, 9000 Rockville Pike, Bethesda MD Y1 - 2007 PY - 2007 DA - 2007 SP - 11 EP - 17 PB - Libertas Academica, PO Box 300-874 VL - 3 SN - 1176-9351, 1176-9351 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Bioinformatics KW - microarrays KW - gene expression KW - biostatistics KW - Gene expression KW - Computer programs KW - software KW - Data processing KW - Statistics KW - Informatics KW - Statistical analysis KW - genomics KW - DNA microarrays KW - W 30910:Imaging KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20663017?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+Informatics&rft.atitle=Analysis+of+Gene+Expression+Data+Using+BRB-Array+Tools&rft.au=Simon%2C+Richard%3BLam%2C+Amy%3BLi%2C+Ming-Chung%3BNgan%2C+Michael%3BMenenzes%2C+Supriya%3BZhao%2C+Yingdong&rft.aulast=Simon&rft.aufirst=Richard&rft.date=2007-01-01&rft.volume=3&rft.issue=&rft.spage=11&rft.isbn=&rft.btitle=&rft.title=Cancer+Informatics&rft.issn=11769351&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Gene expression; Computer programs; software; Statistics; Data processing; Informatics; Statistical analysis; genomics; DNA microarrays ER - TY - JOUR T1 - Architecture of the Bacteriophage T4 Replication Complex Revealed with Nanoscale Biopointers AN - 20610489; 7252655 AB - Our previous electron microscopy of DNA replicated by the bacteriophage T4 proteins showed a single complex at the fork, thought to contain the leading and lagging strand proteins, as well as the protein-covered single-stranded DNA on the lagging strand folded into a compact structure. "Trombone" loops formed from nascent lagging strand fragments were present on a majority of the replicating molecules (Chastain, P., Makhov, A. M., Nossal, N. G., and Griffith, J. D. (2003) J. Biol. Chem. 278, 21276-21285). Here we probe the composition of this replication complex using nanoscale DNA biopointers to show the location of biotin-tagged replication proteins. We find that a large fraction of the molecules with a trombone loop had two pointers to polymerase, providing strong evidence that the leading and lagging strand polymerases are together in the replication complex. 6% of the molecules had two loops, and 31% of these had three pointers to biotin-tagged polymerase, suggesting that the two loops result from two fragments that are being extended simultaneously. Under fixation conditions that extend the lagging strand, occasional molecules show two nascent lagging strand fragments, each being elongated by a biotin-tagged polymerase. T4 41 helicase is present in the complex on a large fraction of actively replicating molecules but on a smaller fraction of molecules with a stalled polymerase. Unexpectedly, we found that 59 helicase-loading protein remains on the fork after loading the helicase and is present on molecules with extensive replication. JF - Journal of Biological Chemistry AU - Nossal, Nancy G AU - Makhov, Alexander M AU - Chastain, Paul DII AU - Jones, Charles E AU - Griffith, Jack D AD - Laboratory of Molecular and Cellular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0830 and the Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1098 EP - 1108 PB - American Society for Biochemistry and Molecular Biology, 9650 Rockville Pike Bethesda MD 20814-3996 USA, [mailto:asbmb@asbmb.faseb.org], [URL:http://www.jbc.org] VL - 282 IS - 2 SN - 0021-9258, 0021-9258 KW - Microbiology Abstracts B: Bacteriology; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Phages KW - Bacteria KW - Replication KW - DNA probes KW - DNA KW - Single-stranded DNA KW - DNA helicase KW - Electron microscopy KW - N 14820:DNA Metabolism & Structure KW - V 22320:Replication KW - J 02430:Symbiosis, Antibiosis & Phages KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20610489?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biological+Chemistry&rft.atitle=Architecture+of+the+Bacteriophage+T4+Replication+Complex+Revealed+with+Nanoscale+Biopointers&rft.au=Nossal%2C+Nancy+G%3BMakhov%2C+Alexander+M%3BChastain%2C+Paul+DII%3BJones%2C+Charles+E%3BGriffith%2C+Jack+D&rft.aulast=Nossal&rft.aufirst=Nancy&rft.date=2007-01-01&rft.volume=282&rft.issue=2&rft.spage=1098&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biological+Chemistry&rft.issn=00219258&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Phages; Replication; DNA probes; DNA; Single-stranded DNA; DNA helicase; Electron microscopy; Bacteria ER - TY - JOUR T1 - Detection of in Vitro Kinase Generated Protein Phosphorylation Sites Using gamma[ super(18)O sub(4)]-ATP and Mass Spectrometry AN - 20595004; 7986221 AB - A novel stable-isotope labeling approach for identification of phosphopeptides that utilizes adenosine triphosphate, in which four oxygen-16 atoms attached to the terminal phosphate group are substituted with oxygen-18 [gamma( super(18)O sub(4))-ATP], has been developed. The ability to use gamma( super(18)O sub(4))-ATP to monitor phosphorylation modification within various proteins was conducted by performing in vitro kinase reactions in the presence of a 1:1 mixture of gamma( super(18)O sub(4))-ATP and normal isotopic abundance ATP (ATP). After tryptic digestion, the peptides were analyzed using mass spectrometry (MS). Phosphorylated peptides are easily recognized within the MS spectrum owing to the presence of doublets separated by 6.01 Da; representing versions of the peptide modified by ATP and gamma( super(18)O sub(4))-ATP. Standard peptides phosphorylated using gamma( super(18)O sub(4))-ATP via in vitro kinase reactions showed no exchange loss of super(18)O with super(16)O. The identity of these doublets as phosphorylated peptides could be readily confirmed using tandem MS. The method described here provides the first direct stable-isotope labeling method to definitely detect phosphorylation sites within proteins. JF - Analytical Chemistry (Washington) AU - Zhou, Ming AU - Meng, Zhaojing AU - Jobson, Andrew G AU - Pommier, Yves AU - Veenstra, Timothy D AD - SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702-1201 Y1 - 2007 PY - 2007 DA - 2007 SP - 7603 EP - 7610 PB - American Chemical Society, Box 3337 Columbus OH 43210 USA, [mailto:service@acs.org] VL - 79 IS - 20 SN - 0003-2700, 0003-2700 KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Phosphorylation KW - Phosphate KW - ATP KW - Mass spectroscopy KW - N 14810:Methods KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20595004?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Analytical+Chemistry+%28Washington%29&rft.atitle=Detection+of+in+Vitro+Kinase+Generated+Protein+Phosphorylation+Sites+Using+gamma%5B+super%2818%29O+sub%284%29%5D-ATP+and+Mass+Spectrometry&rft.au=Zhou%2C+Ming%3BMeng%2C+Zhaojing%3BJobson%2C+Andrew+G%3BPommier%2C+Yves%3BVeenstra%2C+Timothy+D&rft.aulast=Zhou&rft.aufirst=Ming&rft.date=2007-01-01&rft.volume=79&rft.issue=20&rft.spage=7603&rft.isbn=&rft.btitle=&rft.title=Analytical+Chemistry+%28Washington%29&rft.issn=00032700&rft_id=info:doi/10.1021%2Fac071584r LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Phosphate; Phosphorylation; ATP; Mass spectroscopy DO - http://dx.doi.org/10.1021/ac071584r ER - TY - JOUR T1 - Stability of Tubular Structures Based on beta2-Helical Proteins: Self- Assembled versus Polymerized Nanoconstructs and Wild-Type versus Mutated Sequences AN - 20563429; 8019652 AB - In this work we used atomistic molecular dynamics simulations to examine different aspects of tubular nanostructures constructed using protein building blocks with a beta2-helical conformation. Initially, we considered two different natural protein building blocks, which were extracted from the protein data base, to compare the relative stabilities of the nanotubes obtained made of self-assembled and covalently linked repeats. Results show nanotubes constructed by linking building blocks through covalent bonds are very stable suggesting that the basic principles of polymer physics are valid when the repeating units are made of large fragments of proteins. In contrast, the stability of self-assembled nanostructures strongly depends on the attractive nonbonding interactions associated to building blocks aligned in a complementary manner. On the other hand, we investigated the ability of a conformationally constrained synthetic amino acid to enhance the stability of both self-assembled and polymerized nanotubes when it is used to substitute natural residues. Specifically, we considered 1-aminocyclopentane- 1-caboxylic acid, which involves strong stereochemical constraints produced by the cyclopentane side chain. We found that the incorporation of this amino acid within the more flexible regions of the beta2-helical building blocks is an excellent strategy to enhance the stability of the nanotubes. Thus, when a single mutation is performed in the loop region of the beta2-helix, the bend architecture of the whole loop is stabilized since the conformational mobility is reduced not only at the mutated position but also at the adjacent positions. JF - Biomacromolecules AU - Zanuy, David AU - Rodriguez-Ropero, Francisco AU - Haspel, Nurit AU - Zheng, Jie AU - Nussinov, Ruth AU - Aleman, Carlos AD - Departament d'Enginyeria Quimica, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politecnica de Catalunya, Diagonal 647, Barcelona E- 08028, Spain, Rice University, P.O. Box 1892, Houston, Texas 77251-1892, Basic Research Program, SAIC-Frederick Inc., Center for Cancer Research, Nanobiology Program, NCI-FCRDC, Frederick, Maryland 21702 Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 3135 EP - 3146 PB - American Chemical Society, P.O. Box 182426 Columbus OH 43218-2426 USA, [mailto:service@acs.org] VL - 8 IS - 10 SN - 1525-7797, 1525-7797 KW - Biotechnology and Bioengineering Abstracts KW - Databases KW - Amino acids KW - Mobility KW - nanotubes KW - Mutation KW - Cyclopentane KW - W 30935:Food Biotechnology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20563429?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomacromolecules&rft.atitle=Stability+of+Tubular+Structures+Based+on+beta2-Helical+Proteins%3A+Self-+Assembled+versus+Polymerized+Nanoconstructs+and+Wild-Type+versus+Mutated+Sequences&rft.au=Zanuy%2C+David%3BRodriguez-Ropero%2C+Francisco%3BHaspel%2C+Nurit%3BZheng%2C+Jie%3BNussinov%2C+Ruth%3BAleman%2C+Carlos&rft.aulast=Zanuy&rft.aufirst=David&rft.date=2007-01-01&rft.volume=8&rft.issue=10&rft.spage=3135&rft.isbn=&rft.btitle=&rft.title=Biomacromolecules&rft.issn=15257797&rft_id=info:doi/10.1021%2Fbm700561tPII%3AS1525-7797%2870%2900561-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Databases; Amino acids; Mobility; nanotubes; Mutation; Cyclopentane DO - http://dx.doi.org/10.1021/bm700561tPII:S1525-7797(70)00561-5 ER - TY - JOUR T1 - Clinical Applications of Transcranial Doppler Sonography AN - 20558560; 7973523 AB - Transcranial Doppler sonography (TCD) is used to assess cerebral blood flow velocity in basal cerebral arteries and is a common tool for the diagnosis and follow-up of cerebrovascular disease. With more than 200 clinical studies using TCD published annually, indications for its use are expanding. The current article critically reviews standard and recent clinical applications for TCD including delayed vasospasm after subarachnoid hemorrhage, sickle cell disease, atherosclerosis of cranial vessels, ischemic stroke, brain trauma, brain death, carotid artery disease, cerebral venous thrombosis, intraoperative TCD monitoring, arteriovenous malformations, cardiac shunts and preeclampsia. JF - Reviews on Recent Clinical Trials AU - Schatlo, Bawarjan AU - Pluta, Ryszard M AD - Surgical Neurology Branch,NINDS, National Institutes of Health, 10 Center Drive, Room 5D37,Bethesda,MD 20892, USA. Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 49 EP - 57 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 2 IS - 1 SN - 1574-8871, 1574-8871 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Autoregulation KW - Cerebral blood flow KW - Cerebrovascular disease KW - Chemoregulation KW - Transcranial Dopplersonography KW - Stroke KW - subarachnoid hemorrhage KW - Brain injury KW - Vasoconstriction KW - Arteriosclerosis KW - Ischemia KW - Clinical trials KW - Thrombosis KW - Skull KW - Shunts KW - Reviews KW - Pre-eclampsia KW - Carotid artery KW - Traumatic brain injury KW - Sickle cell disease KW - W 30910:Imaging KW - N3 11027:Neurology & neuropathology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20558560?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Reviews+on+Recent+Clinical+Trials&rft.atitle=Clinical+Applications+of+Transcranial+Doppler+Sonography&rft.au=Schatlo%2C+Bawarjan%3BPluta%2C+Ryszard+M&rft.aulast=Schatlo&rft.aufirst=Bawarjan&rft.date=2007-01-01&rft.volume=2&rft.issue=1&rft.spage=49&rft.isbn=&rft.btitle=&rft.title=Reviews+on+Recent+Clinical+Trials&rft.issn=15748871&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - subarachnoid hemorrhage; Brain injury; Stroke; Vasoconstriction; Ischemia; Arteriosclerosis; Clinical trials; Thrombosis; Skull; Reviews; Shunts; Pre-eclampsia; Carotid artery; Traumatic brain injury; Sickle cell disease; Cerebral blood flow ER - TY - JOUR T1 - The Use of Compressor Cycle Patterns: The Ability to Predict Freezer Failure AN - 20556387; 9264173 AB - Biorepositories are tasked with the care and storage of priceless collections of biological specimens. Critical to the success of biorepository operations is the safe, secure, viable storage of the specimens entrusted to its care. The primary means by which this is accomplished is to ensure proper function of ultralow freezers. For a large biorepository this involves the tracking and monitoring of hundreds of freezers. Biorepository procedures typically require recording of the temperature of each freezer on a daily basis. At the Central Repository of the National Cancer Institute in Frederick, Maryland, temperature monitoring has been taken one step further. New procedures utilize the in-house 24-h temperature monitoring system to produce daily printouts of the fluctuation of temperature with time. These graphs are reviewed, and based upon an understanding of freezer compressor cycles are used to identify potential problems with freezer function. These new procedures, which can be used to predict failure, have identified specific types of temperature traces that are indicative of a failure rate 2.9 times greater than normal. JF - Cell Preservation Technology AU - Groover, K AU - Drew, K AU - Franke, J AD - NCI Frederick Central Repository Services Fisher BioServices 4600 Wedgewood Boulevard, Suite H Frederick, MD 21703, USA, kathleen.groover@thermofisher.com Y1 - 2007 PY - 2007 DA - 2007 SP - 225 EP - 228 VL - 5 IS - 4 SN - 1538-344X, 1538-344X KW - Biotechnology and Bioengineering Abstracts KW - Temperature effects KW - Reviews KW - Temperature requirements KW - Preservation KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20556387?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+Preservation+Technology&rft.atitle=The+Use+of+Compressor+Cycle+Patterns%3A+The+Ability+to+Predict+Freezer+Failure&rft.au=Groover%2C+K%3BDrew%2C+K%3BFranke%2C+J&rft.aulast=Groover&rft.aufirst=K&rft.date=2007-01-01&rft.volume=5&rft.issue=4&rft.spage=225&rft.isbn=&rft.btitle=&rft.title=Cell+Preservation+Technology&rft.issn=1538344X&rft_id=info:doi/10.1089%2Fcpt.2007.0510 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Temperature effects; Reviews; Temperature requirements; Preservation DO - http://dx.doi.org/10.1089/cpt.2007.0510 ER - TY - JOUR T1 - Modifications to a Standard Buccal Collection Protocol: Effects on Human DNA Yield AN - 20555448; 9264172 AB - A standard mouthwash protocol (a single 10-mL swish of mouthwash for 45 sec) was modified in an attempt to increase the amount of human buccal cell DNA per collection and to reduce the percentage of low yielding human DNA collections (<4 mu g). A group of 22 healthy individuals donated a buccal sample each week for several weeks according to the standard protocol without or with one of the following modifications: (1) decreasing the volume of mouthwash, (2) having participants externally rub or not rub their cheeks before donating a specimen, (3) donating two consecutive specimens at each collection, (4) substituting saline for mouthwash, and (5) having individuals expectorate into mouthwash. There was no significant difference in the amount of human DNA collected when 10 mL or 5 mL of mouthwash was used. Externally rubbing cheeks before donating did not significantly alter the amount of human DNA collected, regardless of whether it was one or two donations. Addition of a second donation resulted in 24% to 50% more human DNA than from only a single donation, regardless of whether 5 mL or 10 mL of mouthwash was used, with or without cheek rubbing. With two donations, the percentage of low-yielding human DNA samples was reduced up to 31%. Substituting saline for mouthwash resulted in a significantly lower amount of human buccal DNA collected, regardless of cheek rubbing, and a higher number of low-yielding samples. Expectorating directly into mouthwash while externally rubbing cheeks performed the best (17.70 mu g human DNA), followed by swishing 2-10 mL of mouthwash before expectorating (12.26 mu g). Both protocols had 95% of their samples yielding at least 4 mu g of human DNA without increasing cost; however, when selecting a collection protocol the age and health status of the cohort needs to be considered. JF - Cell Preservation Technology AU - Shao, W AU - Garcia-Closas, M AU - Alguacil, J AU - Rothman, N AU - Schatzkin, A AU - Vaught, J AU - Sigurdson, A AU - Cosentino, M AD - 1066 Boyles Street Room 100, P.O. Box B NCI-Frederick Frederick MD 21702, USA, mcosentino@ncifcrf.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 216 EP - 224 VL - 5 IS - 4 SN - 1538-344X, 1538-344X KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Cheek KW - Age KW - Mouthwashes KW - DNA KW - Preservation KW - W 30940:Products KW - N 14845:Miscellaneous UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20555448?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cell+Preservation+Technology&rft.atitle=Modifications+to+a+Standard+Buccal+Collection+Protocol%3A+Effects+on+Human+DNA+Yield&rft.au=Shao%2C+W%3BGarcia-Closas%2C+M%3BAlguacil%2C+J%3BRothman%2C+N%3BSchatzkin%2C+A%3BVaught%2C+J%3BSigurdson%2C+A%3BCosentino%2C+M&rft.aulast=Shao&rft.aufirst=W&rft.date=2007-01-01&rft.volume=5&rft.issue=4&rft.spage=216&rft.isbn=&rft.btitle=&rft.title=Cell+Preservation+Technology&rft.issn=1538344X&rft_id=info:doi/10.1089%2Fcpt.2007.0512 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Cheek; Age; Mouthwashes; DNA; Preservation DO - http://dx.doi.org/10.1089/cpt.2007.0512 ER - TY - JOUR T1 - Synthesis of Cetuximab-Immunoliposomes via a Cholesterol-Based Membrane Anchor for Targeting of EGFR AN - 20526693; 7599370 AB - The objective of the present study was to construct epidermal growth factor receptor (EGFR) targeting cetuximab-immunoliposomes (ILs) for targeted delivery of boron compounds to EGFR(+) glioma cells for neutron capture therapy. The ILs were synthesized by using a novel cholesterol-based membrane anchor, maleimido-PEG-cholesterol (Mal-PEG-Chol), to incorporate cetuximab into liposomes by either surface conjugation or a post-insertion method. For post-insertion, the transfer efficiency of MAb conjugates from micelles to liposome was examined at varying temperatures, mPEG sub(2000)-DSPE ratios, and micelle-to-liposome lipid ratios. Following this, the cetuximab-ILs were evaluated for targeted delivery of the encapsulated boron anion, dodecahydro-closo-dodecaborate (2-) (B sub(12)H super(2) sub(1) super(-) sub(2)), to human EGFR gene transfected F98 sub(EGFR) glioma cells as potential delivery agents for boron neutron capture therapy (BNCT). In addition, cellular uptake of cetuximab-ILs, encapsulating a fluorescence dye, was analyzed by confocal fluorescence microscopy and flow cytometry, and boron content was quantified by ICP-MS. Much greater ( similar to 8-fold) cellular uptake of boron was obtained using cetuximab-ILs in EGFR(+) F98 sub(EGFR) compared with nontargeted human IgG-ILs. On the basis of these observations, we have concluded that cholesterol can serve as an effective anchor for MAb in liposomes, and cetuximab-ILs are potentially useful delivery vehicles for BNCT of gliomas. JF - Bioconjugate Chemistry AU - Pan, X AU - Wu, G AU - Yang, W AU - Barth, R F AU - Tjarks, W AU - Lee, R J AD - Division of Pharmaceutics, College of Pharmacy, NSF Nanoscale Science and Engineering Center (NSEC), Center for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD), Department of Pathology, Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, and NCI Comprehensive Cancer Center (CCC), The Ohio State University, Columbus, Ohio, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 101 EP - 108 VL - 18 IS - 1 SN - 1043-1802, 1043-1802 KW - Biotechnology and Bioengineering Abstracts KW - Temperature effects KW - Anions KW - Monoclonal antibodies KW - Lipids KW - Epidermal growth factor receptors KW - Cholesterol KW - Liposomes KW - Boron KW - Brain tumors KW - Flow cytometry KW - Neutrons KW - Micelles KW - Glioma cells KW - Glioma KW - W 30935:Food Biotechnology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20526693?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioconjugate+Chemistry&rft.atitle=Synthesis+of+Cetuximab-Immunoliposomes+via+a+Cholesterol-Based+Membrane+Anchor+for+Targeting+of+EGFR&rft.au=Pan%2C+X%3BWu%2C+G%3BYang%2C+W%3BBarth%2C+R+F%3BTjarks%2C+W%3BLee%2C+R+J&rft.aulast=Pan&rft.aufirst=X&rft.date=2007-01-01&rft.volume=18&rft.issue=1&rft.spage=101&rft.isbn=&rft.btitle=&rft.title=Bioconjugate+Chemistry&rft.issn=10431802&rft_id=info:doi/10.1021%2Fbc060174r LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Temperature effects; Anions; Monoclonal antibodies; Lipids; Epidermal growth factor receptors; Cholesterol; Boron; Liposomes; Neutrons; Flow cytometry; Brain tumors; Micelles; Glioma cells; Glioma DO - http://dx.doi.org/10.1021/bc060174r ER - TY - JOUR T1 - Prevalence and Variation of Physical Restraint Use in Acute Care Settings in the US AN - 20464027; 9148098 AB - Purpose: To describe physical restraint (PR) rates and contexts in U.S. hospitals.Design: This 2003-2005 descriptive study was done to measure PR prevalence and contexts (census, gender, age, ventilation status, PR type, and rationale) at 40 randomly selected acute care hospitals in six U.S. metropolitan areas. All units except psychiatric, emergency, operative, obstetric, and long-term care were included.Methods: On 18 randomly selected days between 0500 and 0700 (5:00 am and 7:00 am), data collectors determined PR use and contexts via observation and nurse report.Findings: PR prevalence was 50 per 1,000 patient days (based on 155,412 patient days). Preventing disruption of therapy was the chief reason cited. PR rates varied by unit type, with adult ICU rates the highest obtained. Intra- and interinstitutional variation was as high as 10-fold. Ventilator use was strongly associated with PR use. Elderly patients were over-represented among the physically restrained on some units (e.g., medical) but on many unit types (including most ICUs) their PR use was consistent with those of other adults.Conclusions: Wide rate variation indicates the need to examine administratively mediated variables and the promotion of unit-based improvement efforts. Anesthetic and sedation practices have contributed to high variation in ICU PR rates. Determining the types of units to target to achieve improvements in care of older adults requires study of PR sequelae rate by unit type.Journal of Nursing Scholarship, 2007; 39:1, 30-37. [copy2007 Sigma Theta Tau International. JF - Journal of Nursing Scholarship AU - Minnick, Ann F AU - Mion, Lorraine C AU - Johnson, Mary E AU - Catrambone, Cathy AU - Leipzig, Rosanne AD - 1Ann F. Minnick, RN, PhD, FAAN, Gamma Phi, Chenault Professor of Nursing, School of Nursing, Vanderbilt University, Nashville, TN; Lorraine C. Mion, RN, PhD, FAAN, Alpha Mu, Director, Nursing Research, MetroHealth Care Medical Center, Cleveland, OH; Mary E. Johnson, RN, PhD, Associate Professor, College of Nursing, Rush University, Chicago, IL; Cathy Catrambone, RN, DNSc, Instructor, College of Nursing, Rush University, Chicago, IL; Rosanne Leipzig, MD, Professor, Brookdale Department of Geriatrics and Adult Development, Mount Sinai School of Medicine, New York, NY. The project was supported by Grant 1R01AG19715-01 from the National Institute on Aging. Contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the National Institute on Aging, NIH. Correspondence to Dr. Minnick, Vanderbilt University, Godchaux Hall, Room 424, 21st Avenue South, Nashville, TN 37240, Ann.Minnick@vanderbilt.edu Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 30 EP - 37 PB - Blackwell Publishing Ltd., 9600 Garsington Road VL - 39 IS - 1 SN - 1527-6546, 1527-6546 KW - Health & Safety Science Abstracts KW - physical restraints KW - acute care hospitals KW - therapy disruption KW - patient safety KW - census KW - USA KW - Age KW - Ventilation KW - Gender KW - elderly KW - metropolitan areas KW - nursing KW - Medical personnel KW - Hospitals KW - H 13000:Medical Safety UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20464027?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Nursing+Scholarship&rft.atitle=Prevalence+and+Variation+of+Physical+Restraint+Use+in+Acute+Care+Settings+in+the+US&rft.au=Minnick%2C+Ann+F%3BMion%2C+Lorraine+C%3BJohnson%2C+Mary+E%3BCatrambone%2C+Cathy%3BLeipzig%2C+Rosanne&rft.aulast=Minnick&rft.aufirst=Ann&rft.date=2007-01-01&rft.volume=39&rft.issue=1&rft.spage=30&rft.isbn=&rft.btitle=&rft.title=Journal+of+Nursing+Scholarship&rft.issn=15276546&rft_id=info:doi/10.1111%2Fj.1547-5069.2007.00140.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - census; Age; Ventilation; Gender; elderly; nursing; metropolitan areas; Medical personnel; Hospitals; USA DO - http://dx.doi.org/10.1111/j.1547-5069.2007.00140.x ER - TY - JOUR T1 - Preconception maternal polychlorinated biphenyl concentrations and the secondary sex ratio AN - 20389090; 7245528 AB - The secondary sex ratio is the ratio of male to female live births and historically has ranged from 102 to 106 males to 100 females. Temporal declines have been reported in many countries prompting authors to hypothesize an environmental etiology. Blood specimens were obtained from 99 women aged 24-34 prior to attempting pregnancy and quantified for 76 polychlorinated biphenyl (PCB) congeners using dual column gas chromatography with electron capture detection. Women were prospectively followed until pregnancy or 12 cycles of trying. The odds of a male birth for three PCB groupings (total, estrogenic, anti-estrogenic) controlling for maternal characteristics were estimated using logistic regression. Among the 50 women with live births and PCB data, 26 female and 24 male infants were born (ratio 0.92). After adjusting for age and body mass index, odds of a male birth were elevated among women in the second (OR=1.29) and third (OR=1.48) tertiles of estrogenic PCBs; odds (OR=0.70) were reduced among women in the highest tertile of anti-estrogenic PCBs. All confidence intervals included one. The direction of the odds ratios in this preliminary study varied by PCB groupings, supporting the need to study specific PCB patterns when assessing environmental influences on the secondary sex ratio. JF - Environmental Research AU - Taylor, K C AU - Jackson, L W AU - Lynch, C D AU - Kostyniak, P J AU - Buck Louis, GM AD - Statistics and Prevention Research, National Institutes of Child Health and Development (NICHD), NIH, DHHS, 6100 Executive Blvd, Room 7B03, Rockville, MD 20852, USA, louisg@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 99 EP - 105 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 103 IS - 1 SN - 0013-9351, 0013-9351 KW - Pollution Abstracts KW - Historical account KW - Etiology KW - body mass KW - Gas chromatography KW - sex ratio KW - PCB compounds KW - estrogens KW - Pregnancy KW - Infants KW - P 6000:TOXICOLOGY AND HEALTH UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20389090?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Apollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Research&rft.atitle=Preconception+maternal+polychlorinated+biphenyl+concentrations+and+the+secondary+sex+ratio&rft.au=Taylor%2C+K+C%3BJackson%2C+L+W%3BLynch%2C+C+D%3BKostyniak%2C+P+J%3BBuck+Louis%2C+GM&rft.aulast=Taylor&rft.aufirst=K&rft.date=2007-01-01&rft.volume=103&rft.issue=1&rft.spage=99&rft.isbn=&rft.btitle=&rft.title=Environmental+Research&rft.issn=00139351&rft_id=info:doi/10.1016%2Fj.envres.2006.04.009 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Historical account; Etiology; Gas chromatography; body mass; sex ratio; PCB compounds; Infants; Pregnancy; estrogens DO - http://dx.doi.org/10.1016/j.envres.2006.04.009 ER - TY - JOUR T1 - Development of radioimmunotherapeutic and diagnostic antibodies: an inside-out view AN - 20369777; 7730434 AB - Only a handful of radiolabeled antibodies (Abs) have gained US Food and Drag Administration (FDA) approval for use in clinical oncology, including four immunodiagnostic agents and two targeted radioimmunotherapeutic agents. Despite the advent of nonimmunogenic Abs and the availability of a diverse library of radionuclides, progress beyond early Phase II radioimmunotherapy (RIT) studies in solid tumors has been marginal. Furthermore, [ super(18)F]fluorodeoxyglucose continues to dominate the molecular imaging domain, underscored by a decade-long absence of any newly approved Ab-based imaging agent (none since 1996). Why has the development of clinically successful Abs for RIT been limited to lymphoma? What obstacles must be overcome to allow the FDA approval of immuno-positron emission tomography (immuno-PET) imaging agents? How can we address the unique challenges that have thus far prevented the introduction of Ab-based imaging agents and therapeutics for solid tumors? Many poor decisions have been made regarding radiolabeled Abs, but useful insight can be gained from these mistakes. The following review addresses the physical, chemical, biological, clinical, regulatory and financial limitations that impede the progress of this increasingly important class of drugs. JF - Nuclear Medicine and Biology AU - Boswell, CA AU - Brechbiel, M W AD - Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1088, USA, martmwb@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 757 EP - 778 VL - 34 IS - 7 SN - 0969-8051, 0969-8051 KW - Immunology Abstracts; Biotechnology and Bioengineering Abstracts KW - Solid tumors KW - Food KW - Oncology KW - imaging KW - Antibodies KW - Reviews KW - Radioisotopes KW - Tomography KW - Drugs KW - Lymphoma KW - F 06955:Immunomodulation & Immunopharmacology KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20369777?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nuclear+Medicine+and+Biology&rft.atitle=Development+of+radioimmunotherapeutic+and+diagnostic+antibodies%3A+an+inside-out+view&rft.au=Boswell%2C+CA%3BBrechbiel%2C+M+W&rft.aulast=Boswell&rft.aufirst=CA&rft.date=2007-01-01&rft.volume=34&rft.issue=7&rft.spage=757&rft.isbn=&rft.btitle=&rft.title=Nuclear+Medicine+and+Biology&rft.issn=09698051&rft_id=info:doi/10.1016%2Fj.nucmedbio.2007.04.001 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-12-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - imaging; Antibodies; Solid tumors; Reviews; Tomography; Lymphoma; Food; Drugs; Oncology; Radioisotopes DO - http://dx.doi.org/10.1016/j.nucmedbio.2007.04.001 ER - TY - JOUR T1 - Genome-wide association of echocardiographic dimensions, brachial artery endothelial function and treadmill exercise responses in the Framingham Heart Study AN - 20365725; 9029212 AB - Background Echocardiographic left ventricular (LV) measurements, exercise responses to standardized treadmill test (ETT) and brachial artery (BA) vascular function are heritable traits that are associated with cardiovascular disease risk. We conducted a genome-wide association study (GWAS) in the community-based Framingham Heart Study. Methods We estimated multivariable-adjusted residuals for quantitative echocardiography, ETT and BA function traits. Echocardiography residuals were averaged across 4 examinations and included LV mass, diastolic and systolic dimensions, wall thickness, fractional shortening, left atrial and aortic root size. ETT measures (single exam) included systolic blood pressure and heart rate responses during exercise stage 2, and at 3 minutes post-exercise. BA measures (single exam) included vessel diameter, flow-mediated dilation (FMD), and baseline and hyperemic flow responses. Generalized estimating equations (GEE), family-based association tests (FBAT) and variance-components linkage were used to relate multivariable-adjusted trait residuals to 70,987 SNPs (Human 100K GeneChip, Affymetrix) restricted to autosomal SNPs with minor allele frequency greater than or equal to 0.10, genotype call rate greater than or equal to 0.80, and Hardy-Weinberg equilibrium p greater than or equal to 0.001. Results We summarize results from 17 traits in up to 1238 related middle-aged to elderly men and women. Results of all association and linkage analyses are web-posted at . We confirmed modest-to-strong heritabilities (estimates 0.30-0.52) for several Echo, ETT and BA function traits. Overall, p & 10 super(-5 )in either GEE or FBAT models were observed for 21 SNPs (nine for echocardiography, eleven for ETT and one for BA function). The top SNPs associated were (GEE results): LV diastolic dimension, rs1379659 (SLIT2, p = 1.17*10 super(-7)); LV systolic dimension, rs10504543 (KCNB2, p = 5.18*10 super(-6)); LV mass, rs10498091 (p = 5.68*10 super(-6)); Left atrial size, rs1935881 (FAM5C, p = 6.56*10 super(-6)); exercise heart rate, rs6847149 (NOLA1, p = 2.74*10 super(-6)); exercise systolic blood pressure, rs2553268 (WRN, p = 6.3*10 super(-6)); BA baseline flow, rs3814219 (OBFC1, 9.48*10 super(-7)), and FMD, rs4148686 (CFTR, p = 1.13*10 super(-5)). Several SNPs are reasonable biological candidates, with some being related to multiple traits suggesting pleiotropy. The peak LOD score was for LV mass (4.38; chromosome 5); the 1.5 LOD support interval included NRG2. Conclusion In hypothesis-generating GWAS of echocardiography, ETT and BA vascular function in a moderate-sized community-based sample, we identified several SNPs that are candidates for replication attempts and we provide a web-based GWAS resource for the research community. JF - BMC Medical Genetics AU - Vasan, Ramachandran S AU - Larson, Martin G AU - Aragam, Jayashri AU - Wang, Thomas J AU - Mitchell, Gary F AU - Kathiresan, Sekar AU - Newton-Cheh, Christopher AU - Vita, Joseph A AU - Keyes, Michelle J AU - O'Donnell, Christopher J AU - Levy, Daniel AU - Benjamin, Emelia J AD - The National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, MA, USA, vasan@bu.edu Y1 - 2007 PY - 2007 DA - 2007 SP - S2 PB - BioMed Central Ltd., Middlesex House VL - 8 IS - Suppl 1 SN - 1471-2350, 1471-2350 KW - Genetics Abstracts; Physical Education Index KW - Arteries KW - Heart rate KW - Genotypes KW - chromosome 5 KW - Blood pressure KW - Models KW - Ventricle KW - Geriatrics KW - Diseases KW - Treadmill ergometry KW - Vascular system KW - Circulatory system KW - Heart KW - Mathematical models KW - Replication KW - Aorta KW - Echocardiography KW - Exercise KW - Physical training KW - pleiotropy KW - Linkage analysis KW - Vocalization behavior KW - Single-nucleotide polymorphism KW - Reviews KW - Analysis KW - Gene frequency KW - Cardiovascular diseases KW - Heritability KW - G 07880:Human Genetics KW - PE 090:Sports Medicine & Exercise Sport Science UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20365725?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Medical+Genetics&rft.atitle=Genome-wide+association+of+echocardiographic+dimensions%2C+brachial+artery+endothelial+function+and+treadmill+exercise+responses+in+the+Framingham+Heart+Study&rft.au=Vasan%2C+Ramachandran+S%3BLarson%2C+Martin+G%3BAragam%2C+Jayashri%3BWang%2C+Thomas+J%3BMitchell%2C+Gary+F%3BKathiresan%2C+Sekar%3BNewton-Cheh%2C+Christopher%3BVita%2C+Joseph+A%3BKeyes%2C+Michelle+J%3BO%27Donnell%2C+Christopher+J%3BLevy%2C+Daniel%3BBenjamin%2C+Emelia+J&rft.aulast=Vasan&rft.aufirst=Ramachandran&rft.date=2007-01-01&rft.volume=8&rft.issue=Suppl+1&rft.spage=S2&rft.isbn=&rft.btitle=&rft.title=BMC+Medical+Genetics&rft.issn=14712350&rft_id=info:doi/10.1186%2F1471-2350-8-S1-S2 LA - English DB - Physical Education Index N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Heart; Analysis; Heart rate; Echocardiography; Diseases; Exercise; Blood pressure; Treadmill ergometry; Circulatory system; Mathematical models; Replication; Arteries; Aorta; Genotypes; chromosome 5; Models; Physical training; pleiotropy; Ventricle; Linkage analysis; Vocalization behavior; Single-nucleotide polymorphism; Reviews; Geriatrics; Gene frequency; Cardiovascular diseases; Heritability; Vascular system DO - http://dx.doi.org/10.1186/1471-2350-8-S1-S2 ER - TY - JOUR T1 - Genome-wide association to body mass index and waist circumference: the Framingham Heart Study 100K project AN - 20356713; 9029211 AB - Background Obesity is related to multiple cardiovascular disease (CVD) risk factors as well as CVD and has a strong familial component. We tested for association between SNPs on the Affymetrix 100K SNP GeneChip and measures of adiposity in the Framingham Heart Study. Methods A total of 1341 Framingham Heart Study participants in 310 families genotyped with the Affymetrix 100K SNP GeneChip had adiposity traits measured over 30 years of follow up. Body mass index (BMI), waist circumference (WC), weight change, height, and radiographic measures of adiposity (subcutaneous adipose tissue, visceral adipose tissue, waist circumference, sagittal height) were measured at multiple examination cycles. Multivariable-adjusted residuals, adjusting for age, age-squared, sex, smoking, and menopausal status, were evaluated in association with the genotype data using additive Generalized Estimating Equations (GEE) and Family Based Association Test (FBAT) models. We prioritized mean BMI over offspring examinations (1-7) and cohort examinations (10, 16, 18, 20, 22, 24, 26) and mean WC over offspring examinations (4-7) for presentation. We evaluated associations with 70,987 SNPs on autosomes with minor allele frequencies of at least 0.10, Hardy-Weinberg equilibrium p greater than or equal to 0.001, and call rates of at least 80%. Results The top SNPs to be associated with mean BMI and mean WC by GEE were rs110683 (p-value 1.22*10 super(-7)) and rs4471028 (p-values 1.96*10 super(-7)). Please see for the complete set of results. We were able to validate SNPs in known genes that have been related to BMI or other adiposity traits, including the ESR1 Xba1 SNP, PPARG, and ADIPOQ. Conclusion Adiposity traits are associated with SNPs on the Affymetrix 100K SNP GeneChip. Replication of these initial findings is necessary. These data will serve as a resource for replication as more genes become identified with BMI and WC. JF - BMC Medical Genetics AU - Fox, Caroline S AU - Heard-Costa, Nancy AU - Cupples, L Adrienne AU - Dupuis, Josee AU - Vasan, Ramachandran S AU - Atwood, Larry D AD - The National Heart Lung and Blood Institute's Framingham Heart Study, Framingham, MA, USA, foxca@nhlbi.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - S18 PB - BioMed Central Ltd., Middlesex House VL - 8 IS - Suppl 1 SN - 1471-2350, 1471-2350 KW - Genetics Abstracts; Physical Education Index KW - Measurement KW - Age KW - Peroxisome proliferator-activated receptors KW - Body mass KW - Genotypes KW - Models KW - Evaluation KW - Smoking KW - Genetics KW - ESR1 protein KW - Risk factors KW - Heart KW - Obesity KW - Mathematical models KW - Data processing KW - Replication KW - Height KW - Vocalization behavior KW - Waist KW - Single-nucleotide polymorphism KW - Adipose tissue KW - Family KW - Fats KW - Gene frequency KW - Progeny KW - Cardiovascular diseases KW - Body mass index KW - G 07880:Human Genetics KW - PE 090:Sports Medicine & Exercise Sport Science UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20356713?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Medical+Genetics&rft.atitle=Genome-wide+association+to+body+mass+index+and+waist+circumference%3A+the+Framingham+Heart+Study+100K+project&rft.au=Fox%2C+Caroline+S%3BHeard-Costa%2C+Nancy%3BCupples%2C+L+Adrienne%3BDupuis%2C+Josee%3BVasan%2C+Ramachandran+S%3BAtwood%2C+Larry+D&rft.aulast=Fox&rft.aufirst=Caroline&rft.date=2007-01-01&rft.volume=8&rft.issue=Suppl+1&rft.spage=S18&rft.isbn=&rft.btitle=&rft.title=BMC+Medical+Genetics&rft.issn=14712350&rft_id=info:doi/10.1186%2F1471-2350-8-S1-S18 LA - English DB - Physical Education Index N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Heart; Evaluation; Genetics; Measurement; Waist; Body mass; Fats; Family; Height; Obesity; Age; Data processing; Mathematical models; Peroxisome proliferator-activated receptors; Replication; Genotypes; Models; Smoking; ESR1 protein; Vocalization behavior; Single-nucleotide polymorphism; Risk factors; Adipose tissue; Progeny; Gene frequency; Cardiovascular diseases; Body mass index DO - http://dx.doi.org/10.1186/1471-2350-8-S1-S18 ER - TY - JOUR T1 - Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog AN - 20354172; 9030077 AB - Background Genomics tools, particularly DNA microarrays, have found application in a number of areas including gene discovery and disease characterization. Despite the vast utility of these tools, little work has been done to explore the basis of distinct cellular properties, especially those important to biotechnology such as growth. And so, with the intent of engineering cell lines by manipulating the expression of these genes, anchorage-independent and anchorage-dependent HeLa cells, displaying markedly different growth characteristics, were analyzed using DNA microarrays. Results Two genes, cyclin-dependent kinase like 3 (cdkl3) and cytochrome c oxidase subunit (cox15), were up-regulated in the faster growing, anchorage-independent (suspension) HeLa cells relative to the slower growing, anchorage-dependent (attached) HeLa cells. Enhanced expression of either gene in the attached HeLa cells resulted in elevated cell proliferation, though insertion of cdkl3 had a greater impact than that of cox15. Moreover, flow cytometric analysis indicated that cells with an insert of cdkl3 were able to transition from the G0/G1 phases to the S phase faster than control cells. In turn, expression of cox15 was seen to increase the maximum viable cell numbers achieved relative to the control, and to a greater extent than cdkl3. Quantitatively similar results were obtained with two Human Embryonic Kidney-293 (HEK-293) cell lines and a Chinese Hamster Ovary (CHO) cell line. Additionally, HEK-293 cells secreting adipocyte complement-related protein of 30 kDa (acrp30) exhibited a slight increase in specific protein production and higher total protein production in response to the insertion of either cdkl3 or cox15. Conclusion These results are consistent with previous studies on the functionalities of cdkl3 and cox15. For instance, the effect of cdkl3 on cell growth is consistent with its homology to the cdk3 gene which is involved in G1 to S phase transition. Likewise, the increase in cell viability due to cox15 expression is consistent with its role in oxidative phosphorylation as an assembly factor for cytochrome c oxidase and its involvement removing apoptosis-inducing oxygen radicals. Collectively, the present study illustrates the potential of using microarray technology to identify genes influential to specific cellular processes with the possibility of engineering cell lines as desired to meet production needs. JF - BMC Biotechnology AU - Jaluria, Pratik AU - Betenbaugh, Michael AU - Konstantopoulos, Konstantinos AU - Shiloach, Joseph AD - Department of Chemical and Biomolecular Engineering, Johns Hopkins University. 221 Maryland Hall, 3400 North Charles Street, Baltimore, MD 21218 USA, pratikj@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 71 PB - BioMed Central Ltd., Middlesex House VL - 7 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Cell number KW - Oxidative phosphorylation KW - Cytochrome-c oxidase KW - DNA microarrays KW - Flow cytometry KW - Reactive oxygen species KW - Mammalian cells KW - Homology KW - Cyclin-dependent kinase KW - S phase KW - Adipocytes KW - G1 phase KW - Embryos KW - genomics KW - Cell proliferation KW - W 30910:Imaging KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20354172?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Biotechnology&rft.atitle=Enhancement+of+cell+proliferation+in+various+mammalian+cell+lines+by+gene+insertion+of+a+cyclin-dependent+kinase+homolog&rft.au=Jaluria%2C+Pratik%3BBetenbaugh%2C+Michael%3BKonstantopoulos%2C+Konstantinos%3BShiloach%2C+Joseph&rft.aulast=Jaluria&rft.aufirst=Pratik&rft.date=2007-01-01&rft.volume=7&rft.issue=&rft.spage=71&rft.isbn=&rft.btitle=&rft.title=BMC+Biotechnology&rft.issn=1472-6750&rft_id=info:doi/10.1186%2F1472-6750-7-71 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Cell number; Oxidative phosphorylation; Cytochrome-c oxidase; DNA microarrays; Flow cytometry; Cyclin-dependent kinase; Homology; Mammalian cells; Reactive oxygen species; S phase; Adipocytes; G1 phase; Embryos; genomics; Cell proliferation DO - http://dx.doi.org/10.1186/1472-6750-7-71 ER - TY - JOUR T1 - A model-based optimization framework for the inference of regulatory interactions using time-course DNA microarray expression data AN - 20349509; 9022970 AB - Background Proteins are the primary regulatory agents of transcription even though mRNA expression data alone, from systems like DNA microarrays, are widely used. In addition, the regulation process in genetic systems is inherently non-linear in nature, and most studies employ a time-course analysis of mRNA expression. These considerations should be taken into account in the development of methods for the inference of regulatory interactions in genetic networks. Results We use an S-system based model for the transcription and translation process. We propose an optimization-based regulatory network inference approach that uses time-varying data from DNA microarray analysis. Currently, this seems to be the only model-based method that can be used for the analysis of time-course "relative" expressions (expression ratios). We perform an analysis of the dynamic behavior of the system when the number of experimental samples available is varied, when there are different levels of noise in the data and when there are genes that are not considered by the experimenter. Our studies show that the principal factor affecting the ability of a method to infer interactions correctly is the similarity in the time profiles of some or all the genes. The less similar the profiles are to each other the easier it is to infer the interactions. We propose a heuristic method for resolving networks and show that it displays reasonable performance on a synthetic network. Finally, we validate our approach using real experimental data for a chosen subset of genes involved in the sporulation cascade of Bacillus anthracis. We show that the method captures most of the important known interactions between the chosen genes. Conclusion The performance of any inference method for regulatory interactions between genes depends on the noise in the data, the existence of unknown genes affecting the network genes, and the similarity in the time profiles of some or all genes. Though subject to these issues, the inference method proposed in this paper would be useful because of its ability to infer important interactions, the fact that it can be used with time-course DNA microarray data and because it is based on a non-linear model of the process that explicitly accounts for the regulatory role of proteins. JF - BMC Bioinformatics AU - Thomas, Reuben AU - Paredes, Carlos J AU - Mehrotra, Sanjay AU - Hatzimanikatis, Vassily AU - Papoutsakis, Eleftherios T AD - Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA, ThomasR3@niehs.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 228 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Gene expression KW - Translation KW - Data processing KW - Sporulation KW - Transcription KW - Problem solving KW - Bioinformatics KW - Bacillus anthracis KW - DNA microarrays KW - Models KW - J 02320:Cell Biology KW - N 14810:Methods KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20349509?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=A+model-based+optimization+framework+for+the+inference+of+regulatory+interactions+using+time-course+DNA+microarray+expression+data&rft.au=Thomas%2C+Reuben%3BParedes%2C+Carlos+J%3BMehrotra%2C+Sanjay%3BHatzimanikatis%2C+Vassily%3BPapoutsakis%2C+Eleftherios+T&rft.aulast=Thomas&rft.aufirst=Reuben&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=228&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-228 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Gene expression; Translation; Data processing; Sporulation; Problem solving; Transcription; Bioinformatics; DNA microarrays; Models; Bacillus anthracis DO - http://dx.doi.org/10.1186/1471-2105-8-228 ER - TY - JOUR T1 - Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor AN - 20348018; 9023719 AB - Background Ovarian folliculogenesis in mammals is a complex process involving interactions between germ and somatic cells. Carefully orchestrated expression of transcription factors, cell adhesion molecules and growth factors are required for success. We have identified a germ-cell specific, basic helix-loop-helix transcription factor, FIGLA (Factor In the GermLine, Alpha) and demonstrated its involvement in two independent developmental processes: formation of the primordial follicle and coordinate expression of zona pellucida genes. Results Taking advantage of Figla null mouse lines, we have used a combined approach of microarray and Serial Analysis of Gene Expression (SAGE) to identify potential downstream target genes. Using high stringent cutoffs, we find that FIGLA functions as a key regulatory molecule in coordinating expression of the NALP family of genes, genes of known oocyte-specific expression and a set of functionally un-annotated genes. FIGLA also inhibits expression of male germ cell specific genes that might otherwise disrupt normal oogenesis. Conclusion These data implicate FIGLA as a central regulator of oocyte-specific genes that play roles in folliculogenesis, fertilization and early development. JF - BMC Developmental Biology AU - Joshi, Saurabh AU - Davies, Holly AU - Sims, Lauren Porter AU - Levy, Shawn E AU - Dean, Jurrien AD - Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA, saurabhj@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 67 PB - BioMed Central Ltd., Middlesex House VL - 7 KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Serial analysis of gene expression KW - Data processing KW - Follicles KW - Germ cells KW - Somatic cells KW - Helix-loop-helix proteins KW - DNA microarrays KW - Fertilization KW - Zona pellucida KW - Transcription factors KW - Growth factors KW - Oogenesis KW - Cell adhesion molecules KW - G 07730:Development & Cell Cycle KW - N 14810:Methods KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20348018?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Developmental+Biology&rft.atitle=Ovarian+gene+expression+in+the+absence+of+FIGLA%2C+an+oocyte-specific+transcription+factor&rft.au=Joshi%2C+Saurabh%3BDavies%2C+Holly%3BSims%2C+Lauren+Porter%3BLevy%2C+Shawn+E%3BDean%2C+Jurrien&rft.aulast=Joshi&rft.aufirst=Saurabh&rft.date=2007-01-01&rft.volume=7&rft.issue=&rft.spage=67&rft.isbn=&rft.btitle=&rft.title=BMC+Developmental+Biology&rft.issn=1471-213X&rft_id=info:doi/10.1186%2F1471-213X-7-67 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Data processing; Serial analysis of gene expression; Follicles; Germ cells; Somatic cells; DNA microarrays; Helix-loop-helix proteins; Fertilization; Zona pellucida; Transcription factors; Growth factors; Cell adhesion molecules; Oogenesis DO - http://dx.doi.org/10.1186/1471-213X-7-67 ER - TY - JOUR T1 - Accuracy of structure-based sequence alignment of automatic methods AN - 20346301; 9023095 AB - Background Accurate sequence alignments are essential for homology searches and for building three-dimensional structural models of proteins. Since structure is better conserved than sequence, structure alignments have been used to guide sequence alignments and are commonly used as the gold standard for sequence alignment evaluation. Nonetheless, as far as we know, there is no report of a systematic evaluation of pairwise structure alignment programs in terms of the sequence alignment accuracy. Results In this study, we evaluate CE, DaliLite, FAST, LOCK2, MATRAS, SHEBA and VAST in terms of the accuracy of the sequence alignments they produce, using sequence alignments from NCBI's human-curated Conserved Domain Database (CDD) as the standard of truth. We find that 4 to 9% of the residues on average are either not aligned or aligned with more than 8 residues of shift error and that an additional 6 to 14% of residues on average are misaligned by 1-8 residues, depending on the program and the data set used. The fraction of correctly aligned residues generally decreases as the sequence similarity decreases or as the RMSD between the C sub( alpha ) positions of the two structures increases. It varies significantly across CDD superfamilies whether shift error is allowed or not. Also, alignments with different shift errors occur between proteins within the same CDD superfamily, leading to inconsistent alignments between superfamily members. In general, residue pairs that are more than 3.0 Aa apart in the reference alignment are heavily (>= 25% on average) misaligned in the test alignments. In addition, each method shows a different pattern of relative weaknesses for different SCOP classes. CE gives relatively poor results for beta -sheet-containing structures (all- beta , alpha / beta , and alpha + beta classes), DaliLite for "others" class where all but the major four classes are combined, and LOCK2 and VAST for all- beta and "others" classes. Conclusion When the sequence similarity is low, structure-based methods produce better sequence alignments than by using sequence similarities alone. However, current structure-based methods still mis-align 11-19% of the conserved core residues when compared to the human-curated CDD alignments. The alignment quality of each program depends on the protein structural type and similarity, with DaliLite showing the most agreement with CDD on average. JF - BMC Bioinformatics AU - Kim, Changhoon AU - Lee, Byungkook AD - Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute National Institutes of Health, Bethesda, Maryland, USA, kimchan@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 355 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Biotechnology and Bioengineering Abstracts KW - Databases KW - Computer programs KW - Data processing KW - Homology KW - Nucleotide sequence KW - Conserved sequence KW - Bioinformatics KW - Models KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20346301?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=Accuracy+of+structure-based+sequence+alignment+of+automatic+methods&rft.au=Kim%2C+Changhoon%3BLee%2C+Byungkook&rft.aulast=Kim&rft.aufirst=Changhoon&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=355&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-355 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Computer programs; Databases; Data processing; Homology; Nucleotide sequence; Conserved sequence; Bioinformatics; Models DO - http://dx.doi.org/10.1186/1471-2105-8-355 ER - TY - JOUR T1 - Extracting gene expression patterns and identifying co-expressed genes from microarray data reveals biologically responsive processes AN - 20342820; 9023166 AB - Background A common observation in the analysis of gene expression data is that many genes display similarity in their expression patterns and therefore appear to be co-regulated. However, the variation associated with microarray data and the complexity of the experimental designs make the acquisition of co-expressed genes a challenge. We developed a novel method for Extracting microarray gene expression Patterns and Identifying co-expressed Genes, designated as EPIG. The approach utilizes the underlying structure of gene expression data to extract patterns and identify co-expressed genes that are responsive to experimental conditions. Results Through evaluation of the correlations among profiles, the magnitude of variation in gene expression profiles, and profile signal-to-noise ratio's, EPIG extracts a set of patterns representing co-expressed genes. The method is shown to work well with a simulated data set and microarray data obtained from time-series studies of dauer recovery and L1 starvation in C. elegans and after ultraviolet (UV) or ionizing radiation (IR)-induced DNA damage in diploid human fibroblasts. With the simulated data set, EPIG extracted the appropriate number of patterns which were more stable and homogeneous than the set of patterns that were determined using the CLICK or CAST clustering algorithms. However, CLICK performed better than EPIG and CAST with respect to the average correlation between clusters/patterns of the simulated data. With real biological data, EPIG extracted more dauer-specific patterns than CLICK. Furthermore, analysis of the IR/UV data revealed 18 unique patterns and 2661 genes out of approximately 17,000 that were identified as significantly expressed and categorized to the patterns by EPIG. The time-dependent patterns displayed similar and dissimilar responses between IR and UV treatments. Gene Ontology analysis applied to each pattern-related subset of co-expressed genes revealed underlying biological processes affected by IR- and/or UV- induced DNA damage. Conclusion EPIG competed with CLICK and performed better than CAST in extracting patterns from simulated data. EPIG extracted more biological informative patterns and co-expressed genes from both C. elegans and IR/UV-treated human fibroblasts. Using Gene Ontology analysis of the genes in the patterns extracted by EPIG, several key biological categories related to p53-dependent cell cycle control were revealed from the IR/UV data. Among them were mitotic cell cycle, DNA replication, DNA repair, cell cycle checkpoint, and G sub(0)-like status transition. EPIG can be applied to data sets from a variety of experimental designs. JF - BMC Bioinformatics AU - Chou, Jeff W AU - Zhou, Tong AU - Kaufmann, William K AU - Paules, Richard S AU - Bushel, Pierre R AD - Microarray Group, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA, chou@niehs.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 427 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Starvation KW - DNA biosynthesis KW - Data processing KW - Diploids KW - Replication KW - Cell cycle KW - Algorithms KW - DNA repair KW - DNA microarrays KW - Fibroblasts KW - Gene expression KW - DNA damage KW - U.V. radiation KW - Ionizing radiation KW - Bioinformatics KW - N 14820:DNA Metabolism & Structure KW - G 07730:Development & Cell Cycle KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20342820?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=Extracting+gene+expression+patterns+and+identifying+co-expressed+genes+from+microarray+data+reveals+biologically+responsive+processes&rft.au=Chou%2C+Jeff+W%3BZhou%2C+Tong%3BKaufmann%2C+William+K%3BPaules%2C+Richard+S%3BBushel%2C+Pierre+R&rft.aulast=Chou&rft.aufirst=Jeff&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=427&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-427 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Starvation; DNA biosynthesis; Data processing; Diploids; Replication; Cell cycle; Algorithms; DNA repair; DNA microarrays; Fibroblasts; Gene expression; DNA damage; U.V. radiation; Ionizing radiation; Bioinformatics DO - http://dx.doi.org/10.1186/1471-2105-8-427 ER - TY - JOUR T1 - DAVID Knowledgebase: a gene-centered database integrating heterogeneous gene annotation resources to facilitate high-throughput gene functional analysis AN - 20342744; 9023165 AB - Background Due to the complex and distributed nature of biological research, our current biological knowledge is spread over many redundant annotation databases maintained by many independent groups. Analysts usually need to visit many of these bioinformatics databases in order to integrate comprehensive annotation information for their genes, which becomes one of the bottlenecks, particularly for the analytic task associated with a large gene list. Thus, a highly centralized and ready-to-use gene-annotation knowledgebase is in demand for high throughput gene functional analysis. Description The DAVID Knowledgebase is built around the DAVID Gene Concept, a single-linkage method to agglomerate tens of millions of gene/protein identifiers from a variety of public genomic resources into DAVID gene clusters. The grouping of such identifiers improves the cross-reference capability, particularly across NCBI and UniProt systems, enabling more than 40 publicly available functional annotation sources to be comprehensively integrated and centralized by the DAVID gene clusters. The simple, pair-wise, text format files which make up the DAVID Knowledgebase are freely downloadable for various data analysis uses. In addition, a well organized web interface allows users to query different types of heterogeneous annotations in a high-throughput manner. Conclusion The DAVID Knowledgebase is designed to facilitate high throughput gene functional analysis. For a given gene list, it not only provides the quick accessibility to a wide range of heterogeneous annotation data in a centralized location, but also enriches the level of biological information for an individual gene. Moreover, the entire DAVID Knowledgebase is freely downloadable or searchable at . JF - BMC Bioinformatics AU - Sherman, Brad T AU - Huang, Da Wei AU - Tan, Qina AU - Guo, Yongjian AU - Bour, Stephan AU - Liu, David AU - Stephens, Robert AU - Baseler, Michael W AU - Lane, H Clifford AU - Lempicki, Richard A AD - Laboratory of Immunopathogenesis and Bioinformatics, Clinical Services Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD 21702, USA, bsherman@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 426 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Databases KW - Data processing KW - Information processing KW - Gene clusters KW - Proteins KW - Bioinformatics KW - genomics KW - G 07880:Human Genetics KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20342744?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=DAVID+Knowledgebase%3A+a+gene-centered+database+integrating+heterogeneous+gene+annotation+resources+to+facilitate+high-throughput+gene+functional+analysis&rft.au=Sherman%2C+Brad+T%3BHuang%2C+Da+Wei%3BTan%2C+Qina%3BGuo%2C+Yongjian%3BBour%2C+Stephan%3BLiu%2C+David%3BStephens%2C+Robert%3BBaseler%2C+Michael+W%3BLane%2C+H+Clifford%3BLempicki%2C+Richard+A&rft.aulast=Sherman&rft.aufirst=Brad&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=426&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-426 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Databases; Data processing; Information processing; Gene clusters; Proteins; genomics; Bioinformatics DO - http://dx.doi.org/10.1186/1471-2105-8-426 ER - TY - JOUR T1 - SEARCHPATTOOL: a new method for mining the most specific frequent patterns for binding sites with application to prokaryotic DNA sequences AN - 20341722; 9023094 AB - Background Computational methods to predict transcription factor binding sites (TFBS) based on exhaustive algorithms are guaranteed to find the best patterns but are often limited to short ones or impose some constraints on the pattern type. Many patterns for binding sites in prokaryotic species are not well characterized but are known to be large, between 16-30 base pairs (bp) and contain at least 2 conserved bases. The length of prokaryotic species promoters (about 400 bp) and our interest in studying a small set of genes that could be a cluster of co-regulated genes from microarray experiments led to the development of a new exhaustive algorithm targeting these large patterns. Results We present Searchpattool, a new method to search for and select the most specific (conservative) frequent patterns. This method does not impose restrictions on the density or the structure of the pattern. The best patterns (motifs) are selected using several statistics, including a new application of a z-score based on the number of matching sequences. We compared Searchpattool against other well known algorithms on a Bacillus subtilis group of 14 input sequences and found that in our experiments Searchpattool always performed the best based on performance scores. Conclusion Searchpattool is a new method for pattern discovery relative to transcription factor binding sites for species or genes with short promoters. It outputs the most specific significant patterns and helps the biologist to choose the best candidates. JF - BMC Bioinformatics AU - Elloumi, Fathi AU - Nason, Martha AD - Research Technology Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Blg50/R5505, Bethesda, MD 20892, USA, elloumi.fathi@epa.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 354 PB - BioMed Central Ltd., Middlesex House VL - 8 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Promoters KW - Bacillus subtilis KW - Statistics KW - Transcription factors KW - Nucleotide sequence KW - Algorithms KW - Bioinformatics KW - Computer applications KW - DNA microarrays KW - Base pairs KW - N 14810:Methods KW - W 30960:Bioinformatics & Computer Applications KW - J 02300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20341722?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=SEARCHPATTOOL%3A+a+new+method+for+mining+the+most+specific+frequent+patterns+for+binding+sites+with+application+to+prokaryotic+DNA+sequences&rft.au=Elloumi%2C+Fathi%3BNason%2C+Martha&rft.aulast=Elloumi&rft.aufirst=Fathi&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=354&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=1471-2105&rft_id=info:doi/10.1186%2F1471-2105-8-354 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Promoters; Statistics; Nucleotide sequence; Transcription factors; Algorithms; Bioinformatics; Computer applications; DNA microarrays; Base pairs; Bacillus subtilis DO - http://dx.doi.org/10.1186/1471-2105-8-354 ER - TY - JOUR T1 - Radioligand Development for PET Imaging of beta -Amyloid (A beta )-Current Status AN - 20337349; 7653320 AB - Two of the main pathological hallmarks of Alzheimers disease (AD) are neuritic plaques and neurofibrillary tangles. Significant evidence supports a critical and probable causative role of beta amyloid (A beta ) plaque formation. Since neuroprotective treatments are typically most effective at early stages of injury, the detection and measurement of A beta load in living brain should be performed at early and perhaps even presymptomatic stages of AD. Two primary targets of molecular imaging research with positron emission tomography (PET) are to develop surrogate markers (radioligands) for assessing disease progression and for monitoring the efficacy of developmental therapeutics. Here, we review the current status of radioligand development for PET imaging of A beta aggregates. General structure-activity relationships have emerged, including the identification of at least three different ligand binding sites in various A beta aggregates and recognition of the general structural requirements for ligand binding at each site. Also a few radioligands applicable to imaging A beta plaques in living human brain with positron emission tomography (PET) have emerged, including [11C]PIB, [11C]SB- 13 and [18F]FDDNP. JF - Current Medicinal Chemistry AU - Cai, Lisheng AU - Innis, Robert B AU - Pike, Victor W AD - Molecular Imaging Branch,National Institute of Mental Health, National Institutes of Health, Bethesda,MD 20892, USA. Y1 - 2007 PY - 2007 DA - 2007 SP - 19 EP - 52 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 14 IS - 1 SN - 0929-8673, 0929-8673 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - PET imaging KW - beta -amyloid KW - radioligand KW - assay KW - Alzheimers disease KW - Neuroimaging KW - Brain injury KW - Alzheimer's disease KW - Brain KW - Neuroprotection KW - Neurodegenerative diseases KW - Reviews KW - Computed tomography KW - Positron emission tomography KW - Plaques KW - Neurofibrillary tangles KW - beta -Amyloid KW - Structure-activity relationships KW - W 30910:Imaging KW - N3 11028:Neuropharmacology & toxicology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20337349?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Medicinal+Chemistry&rft.atitle=Radioligand+Development+for+PET+Imaging+of+beta+-Amyloid+%28A+beta+%29-Current+Status&rft.au=Cai%2C+Lisheng%3BInnis%2C+Robert+B%3BPike%2C+Victor+W&rft.aulast=Cai&rft.aufirst=Lisheng&rft.date=2007-01-01&rft.volume=14&rft.issue=1&rft.spage=19&rft.isbn=&rft.btitle=&rft.title=Current+Medicinal+Chemistry&rft.issn=09298673&rft_id=info:doi/10.2174%2F092986707779313471 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Neuroimaging; Brain injury; Alzheimer's disease; Brain; Neuroprotection; Neurodegenerative diseases; Reviews; Computed tomography; Positron emission tomography; Plaques; beta -Amyloid; Neurofibrillary tangles; Structure-activity relationships DO - http://dx.doi.org/10.2174/092986707779313471 ER - TY - JOUR T1 - Rational Modifications of HIV-1 Envelope Glycoproteins for Immunogen Design AN - 20318255; 7654936 AB - An effective vaccine against the human immunodeficiency virus type 1 (HIV-1) will likely require the elicitation of broadly neutralizing antibodies as well as cellular responses. The HIV exterior envelope glycoprotein trimers, gp120, and the transmembrane glycoprotein, gp41, mediate entry and are the sole viral targets for neutralizing antibodies. However, as subunit immunogens the envelope glycoproteins do not efficiently elicit antibodies capable of neutralizing the extremely diverse array of viruses circulating in the human population. The preponderance of data suggest that inefficient generation of broadly neutralizing antibodies is due to naturally evolved mechanisms of immune evasion inherent in the unmodified HIV envelope glycoproteins. Because the established modes of anti-viral vaccine development, liveattenuation and virus inactivation have not yet been successful for HIV, we and others have focused on subunit vaccine design. In this review, we describe current approaches of rational modification of the envelope glycoproteins based upon structure, antigenicity, biochemistry and biophysics to alter the properties of the envelope glycoproteins such that, as subunit immunogens, they now better elicit broadly neutralizing antibodies. The application of structure-assisted, rational subunit vaccine design may be a general paradigm for future efforts to develop vaccines against emerging human pathogens. JF - Current Pharmaceutical Design AU - Phogat, S AU - Wyatt, R AD - Structural Virology Section,Vaccine Research Center, National Institutes of Health, 40 Convent Dr.,Bldg. 40, Room 4512, Bethesda, MD 20892-3005, USA. Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 213 EP - 227 PB - Bentham Science Publishers B.V., P.O. Box 1673 Hilversum 1200 BR The Netherlands, [mailto:shidding@worldonline.nl], [URL:http://www.bentham.org] VL - 13 IS - 2 SN - 1381-6128, 1381-6128 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - HIV vaccine KW - envelope glycoproteins KW - structure-assisted immunogen design KW - Data processing KW - glycoprotein gp41 KW - Antigenicity KW - Pathogens KW - Biophysics KW - Glycoprotein gp120 KW - Antibodies KW - Envelopes KW - Antiviral agents KW - Reviews KW - Human immunodeficiency virus 1 KW - Vaccines KW - V 22360:AIDS and HIV KW - F 06955:Immunomodulation & Immunopharmacology KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20318255?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Current+Pharmaceutical+Design&rft.atitle=Rational+Modifications+of+HIV-1+Envelope+Glycoproteins+for+Immunogen+Design&rft.au=Phogat%2C+S%3BWyatt%2C+R&rft.aulast=Phogat&rft.aufirst=S&rft.date=2007-01-01&rft.volume=13&rft.issue=2&rft.spage=213&rft.isbn=&rft.btitle=&rft.title=Current+Pharmaceutical+Design&rft.issn=13816128&rft_id=info:doi/10.2174%2F138161207779313632 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-11-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Glycoprotein gp120; Antibodies; Data processing; Envelopes; glycoprotein gp41; Antiviral agents; Antigenicity; Reviews; Pathogens; Vaccines; Biophysics; Human immunodeficiency virus 1 DO - http://dx.doi.org/10.2174/138161207779313632 ER - TY - JOUR T1 - Exercise Capacity of Cardiac Asymptomatic Hereditary Hemochromatosis Subjects AN - 20254667; 7300969 AB - Purpose: The exercise capacity of cardiac asymptomatic subjects with hereditary hemochromatosis (HH) has not been well described. In this study, we tested whether the iron overload associated with HH affected exercise capacity with a case control study design. Methods: Forty-three HH and 21 normal control subjects who were New York Heart Association functional class I underwent metabolic stress testing using the Bruce protocol at the clinical center of the National Institutes of Health. Exercise capacity was assessed with minute ventilation (V sub(E)), oxygen uptake (VO sub(2)), and carbon dioxide production (VCO sub(2)) using a breath-by-breath respiratory gas analyzer. Results: The exercise capacity of HH subjects was not statistically different from that of control subjects (exercise time 564 plus or minus 135 vs 673 plus or minus 175 s, P = 0.191; peak VO sub(2) 29.6 plus or minus 6.4 vs 32.5 plus or minus 6.7 mL times kg super(-1) times min super(-1), P = 0.109; ventilatory threshold 19.0 plus or minus 3.4 vs 21.0 plus or minus 5.0 mL times min super(-1) times kg super(-1), P = 0.099; data are for HH vs control subjects). Ventilatory efficiency was comparable between groups (V sub(E)/VCO sub(2) slope 23.7 plus or minus 3.2 vs 23.4 plus or minus 4.2, P = 0.791). No significant correlation between the markers of iron levels and the markers of exercise capacity was noted. Iron depletion by 6-month phlebotomy therapy in 18 subjects who were newly diagnosed did not affect exercise testing variables (exercise time 562 plus or minus 119 vs 579 plus or minus 118 s, P = 0.691; peak VO sub(2) 29.5 plus or minus 3.7 vs 29.1 plus or minus 4.7 mL times kg super(-1) times min super(-1), P = 0.600; ventilatory threshold 18.5 plus or minus 2.8 vs 17.9 plus or minus 3.8 mL times kg super(-1) times min super(-1), P = 0.651; data are from before and after phlebotomy therapy). Abnormal ischemic electrocardiographic responses and complex arrhythmias were more frequently seen in HH subjects. Conclusions: The aerobic exercise capacity of asymptomatic HH subjects seems not to be statistically different from that of normal subjects. The iron levels do not seem to affect exercise capacity in asymptomatic HH subjects. JF - Medicine & Science in Sports & Exercise AU - Shizukuda, Y AU - Bolan, C D AU - Tripodi, D J AU - Yau, Y-Y AU - Smith, K P AU - Arena, R AU - Waclawiw, MA AU - Leitman, S F AU - Rosing AD - Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10-CRC, Rm. 6-3142, MSC-1454, 10 Center Drive, Bethesda, MD, 20892, USA, shizukuy@nhlbi.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 3 EP - 7 VL - 39 IS - 1 SN - 0195-9131, 0195-9131 KW - Physical Education Index KW - Heart KW - Statistics KW - Aerobics KW - Pulmonary ventilation KW - Therapy KW - Stress KW - Sport science KW - Health KW - Exercise KW - Efficiency KW - Ventilatory threshold KW - Carbon dioxide KW - Maximum oxygen consumption KW - Iron KW - PE 090:Sports Medicine & Exercise Sport Science UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20254667?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Medicine+%26+Science+in+Sports+%26+Exercise&rft.atitle=Exercise+Capacity+of+Cardiac+Asymptomatic+Hereditary+Hemochromatosis+Subjects&rft.au=Shizukuda%2C+Y%3BBolan%2C+C+D%3BTripodi%2C+D+J%3BYau%2C+Y-Y%3BSmith%2C+K+P%3BArena%2C+R%3BWaclawiw%2C+MA%3BLeitman%2C+S+F%3BRosing&rft.aulast=Shizukuda&rft.aufirst=Y&rft.date=2007-01-01&rft.volume=39&rft.issue=1&rft.spage=3&rft.isbn=&rft.btitle=&rft.title=Medicine+%26+Science+in+Sports+%26+Exercise&rft.issn=01959131&rft_id=info:doi/10.1249%2F01.mss.0000240323.08406.f3 LA - English DB - Physical Education Index N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Heart; Statistics; Aerobics; Pulmonary ventilation; Therapy; Sport science; Stress; Health; Exercise; Efficiency; Ventilatory threshold; Carbon dioxide; Iron; Maximum oxygen consumption DO - http://dx.doi.org/10.1249/01.mss.0000240323.08406.f3 ER - TY - JOUR T1 - RNAi Therapy for HIV Infection: Principles and Practicalities AN - 20251749; 7478977 AB - Inside eukaryotic cells, small RNA duplexes, called small interfering RNAs (siRNAs), activate a conserved RNA interference (RNAi) pathway which leads to specific degradation of complementary target mRNAs through base-pairing recognition. As with other viruses, studies have shown that replication of the HIV-1 in cultured cells can be targeted and inhibited by synthetic siRNAs. The relative ease of siRNA design and the versatility of RNAi to target a broad spectrum of mRNAs have led to the promise that drug discovery in the RNAi pathway could be effective against pathogens. This review discusses the current experimental principles that guide the application of RNAi against HIV and describes challenges and limitations that need to be surmounted in order for siRNAs to become practical antiviral drugs. The practical use of RNAi therapy for HIV infection will depend on overcoming several challenges, including the ability to establish long-term expression of siRNA without off-target effects and the capacity to counteract mutant escape viruses. JF - BioDrugs AU - Bennasser, Yamina AU - Man Lung Yeung, AU - Jeang, Kuan-Teh AD - Molecular Virology Section, Laboratory of Molecular Microbiology National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2007 PY - 2007 DA - 2007 SP - 17 EP - 22 PB - Adis International Ltd., 41 Centorian Drive Private Bay 65901, Mairangi Bay Auckland 10 New Zealand, [mailto:sportsmed@adis.co.nz], [URL:http://www.adis.com] VL - 21 IS - 1 SN - 1173-8804, 1173-8804 KW - Virology & AIDS Abstracts; Biotechnology Research Abstracts (through 1992) KW - Replication KW - Drug development KW - Pathogens KW - Infection KW - mRNA KW - Drug discovery KW - Antiviral agents KW - siRNA KW - Reviews KW - Human immunodeficiency virus 1 KW - RNA-mediated interference KW - V 22360:AIDS and HIV KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20251749?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BioDrugs&rft.atitle=RNAi+Therapy+for+HIV+Infection%3A+Principles+and+Practicalities&rft.au=Bennasser%2C+Yamina%3BMan+Lung+Yeung%2C%3BJeang%2C+Kuan-Teh&rft.aulast=Bennasser&rft.aufirst=Yamina&rft.date=2007-01-01&rft.volume=21&rft.issue=1&rft.spage=17&rft.isbn=&rft.btitle=&rft.title=BioDrugs&rft.issn=11738804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Human immunodeficiency virus 1; siRNA; RNA-mediated interference; mRNA; Infection; Drug discovery; Drug development; Pathogens; Replication; Reviews; Antiviral agents ER - TY - JOUR T1 - Sentra: a database of signal transduction proteins for comparative genome analysis AN - 20245598; 7254414 AB - Sentra (http://compbio.mcs.anl.gov/sentra), a database of signal transduction proteins encoded in completely sequenced prokaryotic genomes, has been updated to reflect recent advances in understanding signal transduction events on a whole-genome scale. Sentra consists of two principal components, a manually curated list of signal transduction proteins in 202 completely sequenced prokaryotic genomes and an automatically generated listing of predicted signaling proteins in 235 sequenced genomes that are awaiting manual curation. In addition to two-component histidine kinases and response regulators, the database now lists manually curated Ser/Thr/Tyr protein kinases and protein phosphatases, as well as adenylate and diguanylate cyclases and c-di-GMP phosphodiesterases, as defined in several recent reviews. All entries in Sentra are extensively annotated with relevant information from public databases (e.g. UniProt, KEGG, PDB and NCBI). Sentra's infrastructure was redesigned to support interactive cross-genome comparisons of signal transduction capabilities of prokaryotic organisms from a taxonomic and phenotypic perspective and in the framework of signal transduction pathways from KEGG. Sentra leverages the PUMA2 system to support interactive analysis and annotation of signal transduction proteins by the users. JF - Nucleic Acids Research AU - D'Souza, Mark AU - Glass, Elizabeth M AU - Syed, Mustafa H AU - Zhang, Yi AU - Rodriguez, Alexis AU - Maltsev, Natalia AU - Galperin, Michael Y AD - Computational Biology Group, Mathematics and Computer Science Division, Argonne National Laboratory Argonne, IL 60439, USA. Computation Institute, University of Chicago Chicago, IL 60637, USA. National Center for Biotechnology Information, National Library of Medicine MSC3830, National Institutes of Health, Bethesda, MD 20894, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D271 EP - D273 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Histidine kinase KW - Databases KW - Reviews KW - Protein kinase KW - protein phosphatase KW - phosphodiesterase KW - Signal transduction KW - G 07740:Evolution KW - N 14845:Miscellaneous KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20245598?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=Sentra%3A+a+database+of+signal+transduction+proteins+for+comparative+genome+analysis&rft.au=D%27Souza%2C+Mark%3BGlass%2C+Elizabeth+M%3BSyed%2C+Mustafa+H%3BZhang%2C+Yi%3BRodriguez%2C+Alexis%3BMaltsev%2C+Natalia%3BGalperin%2C+Michael+Y&rft.aulast=D%27Souza&rft.aufirst=Mark&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D271&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Histidine kinase; Genomes; Databases; Reviews; Protein kinase; protein phosphatase; phosphodiesterase; Signal transduction ER - TY - JOUR T1 - Critical Review of Published Microarray Studies for Cancer Outcome and Guidelines on Statistical Analysis and Reporting AN - 20226101; 7253589 AB - BACKGROUND: Both the validity and the reproducibility of microarray-based clinical research have been challenged. There is a need for critical review of the statistical analysis and reporting in published microarray studies that focus on cancer-related clinical outcomes. METHODS: Studies published through 2004 in which microarray-based gene expression profiles were analyzed for their relation to a clinical cancer outcome were identified through a Medline search followed by hand screening of abstracts and full text articles. Studies that were eligible for our analysis addressed one or more outcomes that were either an event occurring during follow-up, such as death or relapse, or a therapeutic response. We recorded descriptive characteristics for all the selected studies. A critical review of outcome-related statistical analyses was undertaken for the articles published in 2004. RESULTS: Ninety studies were identified, and their descriptive characteristics are presented. Sixty-eight (76%) were published in journals of impact factor greater than 6. A detailed account of the 42 studies (47%) published in 2004 is reported. Twenty-one (50%) of them contained at least one of the following three basic flaws: 1) in outcome-related gene finding, an unstated, unclear, or inadequate control for multiple testing; 2) in class discovery, a spurious claim of correlation between clusters and clinical outcome, made after clustering samples using a selection of outcome-related differentially expressed genes; or 3) in supervised prediction, a biased estimation of the prediction accuracy through an incorrect cross-validation procedure. CONCLUSIONS: The most common and serious mistakes and misunderstandings recorded in published studies are described and illustrated. Based on this analysis, a proposal of guidelines for statistical analysis and reporting for clinical microarray studies, presented as a checklist of "Do's and Don'ts," is provided. JF - Journal of the National Cancer Institute AU - Dupuy, Alain AU - Simon, Richard M AD - Biometric Research Branch, Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, MD (AD, RMS) Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 147 EP - 157 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 99 IS - 2 SN - 0027-8874, 0027-8874 KW - Biotechnology and Bioengineering Abstracts KW - Gene expression KW - Reviews KW - Statistical analysis KW - Check lists KW - Hand KW - DNA microarrays KW - Cancer KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20226101?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+the+National+Cancer+Institute&rft.atitle=Critical+Review+of+Published+Microarray+Studies+for+Cancer+Outcome+and+Guidelines+on+Statistical+Analysis+and+Reporting&rft.au=Dupuy%2C+Alain%3BSimon%2C+Richard+M&rft.aulast=Dupuy&rft.aufirst=Alain&rft.date=2007-01-01&rft.volume=99&rft.issue=2&rft.spage=147&rft.isbn=&rft.btitle=&rft.title=Journal+of+the+National+Cancer+Institute&rft.issn=00278874&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-09-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Gene expression; Reviews; Statistical analysis; Hand; Check lists; DNA microarrays; Cancer ER - TY - JOUR T1 - Elicitation of T Cell Responses to Histologically Unrelated Tumors by Immunization with the Novel Cancer-Testis Antigen, Brother of the Regulator of Imprinted Sites AN - 20136920; 7209010 AB - Brother of the regulator of imprinted sites (BORIS) was previously described as a transcription factor for epigenetic reprogramming the expression of which is strictly confined to germ cells of adult testes but is aberrantly activated in the vast majority of neoplastic cells. Considering the critical role of BORIS in cancerogenesis and the fact that its expression pattern may preclude thymic tolerance, we generated DNA- and protein-based mouse BORIS antitumor vaccines using a non-DNA-binding version of the BORIS molecule. Clinical use of BORIS as a vaccine Ag would require that certain safety concerns be met. Specifically, administration of the functional BORIS protein would hypothetically pose a risk of BORIS accelerating the progression of cancer. To alleviate such safety concerns, we have developed vaccines based on the BORIS molecule lacking the DNA-binding zinc fingers domain. To enhance anti-BORIS cellular immune responses, we used a standard molecular adjuvant approach. It consisted of plasmids encoding murine IL-12 and IL-18 for a DNA-based vaccine and conventional Th1 type adjuvant, Quil A, for a protein-based vaccine. Both DNA- and protein-based vaccines induced Ag-specific CD4 super(+) T cell proliferation with Th1 and Th2 cytokine profiles, respectively. Protein-based, but not DNA-based, BORIS vaccine induced a significant level of Ab production in immunized animals. Importantly, potent anticancer CD8 super(+)-cytotoxic lymphocytes were generated after immunization with the DNA-based, but not protein-based, BORIS vaccine. These cytolytic responses were observed across a wide range of different mouse cancers including mammary adenocarcinoma, glioma, leukemia, and mastocytoma. JF - Journal of Immunology AU - Ghochikyan, Anahit AU - Mkrtichyan, Mikayel AU - Loukinov, Dmitri AU - Mamikonyan, Gregory AU - Pack, Svetlana D AU - Movsesyan, Nina AU - Ichim, Thomas E AU - Cribbs, David H AU - Lobanenkov, Victor V AU - Agadjanyan, Michael G AD - Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647. Department of Neurology, Institute for Brain Aging and Dementia, University of California, Irvine, CA 92697. Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852. OncoMune Inc., Miami, FL 33122 Y1 - 2007/01/01/ PY - 2007 DA - 2007 Jan 01 SP - 566 EP - 573 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 178 IS - 1 SN - 0022-1767, 0022-1767 KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Immunology Abstracts KW - Testes KW - Helper cells KW - mastocytoma KW - Zinc finger proteins KW - Adjuvants KW - Leukemia KW - Interleukin 12 KW - CD4 antigen KW - DNA vaccines KW - epigenetics KW - Interleukin 18 KW - Lymphocytes T KW - Glioma KW - Thymus KW - Germ cells KW - Tumors KW - Plasmids KW - Immunological tolerance KW - Immunization KW - Brain tumors KW - Antibodies KW - Transcription factors KW - Vaccines KW - Adenocarcinoma KW - Cell proliferation KW - F 06915:Cancer Immunology KW - W 30915:Pharmaceuticals & Vaccines KW - N 14825:Gene Regulation UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20136920?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Elicitation+of+T+Cell+Responses+to+Histologically+Unrelated+Tumors+by+Immunization+with+the+Novel+Cancer-Testis+Antigen%2C+Brother+of+the+Regulator+of+Imprinted+Sites&rft.au=Ghochikyan%2C+Anahit%3BMkrtichyan%2C+Mikayel%3BLoukinov%2C+Dmitri%3BMamikonyan%2C+Gregory%3BPack%2C+Svetlana+D%3BMovsesyan%2C+Nina%3BIchim%2C+Thomas+E%3BCribbs%2C+David+H%3BLobanenkov%2C+Victor+V%3BAgadjanyan%2C+Michael+G&rft.aulast=Ghochikyan&rft.aufirst=Anahit&rft.date=2007-01-01&rft.volume=178&rft.issue=1&rft.spage=566&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Testes; Helper cells; mastocytoma; Zinc finger proteins; Adjuvants; Interleukin 12; Leukemia; CD4 antigen; DNA vaccines; epigenetics; Interleukin 18; Lymphocytes T; Glioma; Thymus; Germ cells; Tumors; Plasmids; Immunological tolerance; Immunization; Brain tumors; Antibodies; Transcription factors; Vaccines; Cell proliferation; Adenocarcinoma ER - TY - JOUR T1 - NATsDB: Natural Antisense Transcripts DataBase AN - 20129258; 7254386 AB - Natural antisense transcripts (NATs) are reverse complementary at least in part to the sequences of other endogenous sense transcripts. Most NATs are transcribed from opposite strands of their sense partners. They regulate sense genes at multiple levels and are implicated in various diseases. Using an improved whole-genome computational pipeline, we identified abundant cis-encoded exon-overlapping sense-antisense (SA) gene pairs in human (7356), mouse (6806), fly (1554), and eight other eukaryotic species (total 6534). We developed NATsDB (Natural Antisense Transcripts DataBase, http://natsdb.cbi.pku.edu.cn/) to enable efficient browsing, searching and downloading of this currently most comprehensive collection of SA genes, grouped into six classes based on their overlapping patterns. NATsDB also includes non-exon-overlapping bidirectional (NOB) genes and non-bidirectional (NBD) genes. To facilitate the study of functions, regulations and possible pathological implications, NATsDB includes extensive information about gene structures, poly(A) signals and tails, phastCons conservation, homologues in other species, repeat elements, expressed sequence tag (EST) expression profiles and OMIM disease association. NATsDB supports interactive graphical display of the alignment of all supporting EST and mRNA transcripts of the SA and NOB genes to the genomic loci. It supports advanced search by species, gene name, sequence accession number, chromosome location, coding potential, OMIM association and sequence similarity. JF - Nucleic Acids Research AU - Zhang, Yong AU - Li, Jiongtang AU - Kong, Lei AU - Gao, Ge AU - Liu, Qing-Rong AU - Wei, Liping AD - Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University Beijing 100871, PR China. Molecular Neurobiology Branch, National Institute on Drug Abuse-Intramural Research Program (NIDA-IRP), NIH, Department of Health and Human Services (DHHS) Box 5180, Baltimore, MD 21224, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D156 EP - D161 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Biotechnology and Bioengineering Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - Tails KW - Computer applications KW - expressed sequence tags KW - double prime sA gene KW - Gene expression KW - Databases KW - Antisense KW - Chromosomes KW - sA gene KW - Browsing KW - Conserved sequence KW - genomics KW - N 14840:Antisense, Nucleotide Analogs KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20129258?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=NATsDB%3A+Natural+Antisense+Transcripts+DataBase&rft.au=Zhang%2C+Yong%3BLi%2C+Jiongtang%3BKong%2C+Lei%3BGao%2C+Ge%3BLiu%2C+Qing-Rong%3BWei%2C+Liping&rft.aulast=Zhang&rft.aufirst=Yong&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D156&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Gene expression; Databases; Chromosomes; Antisense; sA gene; Tails; Browsing; Conserved sequence; genomics; Computer applications; expressed sequence tags; double prime sA gene ER - TY - JOUR T1 - Gene Expression Signatures of Interleukin-2 In Vivo and In Vitro and Their Relation to Anticancer Therapy AN - 20097710; 7768150 AB - Treatment with human recombinant interleukin-2 (rIL-2) can successfully eradicate advanced cancer in humans; however, its utilization is limited by the unpredictability of its effectiveness and the excessive toxicity often associated with its use. The mechanisms responsible for immune-mediated tumor regression and those associated with limiting toxicity have not yet been sorted out. Thus, this review critically addresses what has been done in the past to understand this biologically and practically important question and discusses future strategies to enhance the understanding of this interesting model of immune-mediated tumor rejection. In particular, the first aim of this review is to discuss what is known about the mechanism(s) responsible for tumor rejection; the second aim is to review the relationship between the toxicity induced by rIL-2 treatment and its effectiveness; the third aim is to summarize novel insights into the possible mechanism of rIL-2 activity in vivo using high-throughput strategies aimed at the global assessment in real-time of events associated with rIL-2 therapy in humans. This information will not only lead to a better utilization of this biological agent in clinical practice but it may also provide important information about how immune-mediated tissue rejection occurs. JF - Critical Reviews in Immunology AU - Jin, P AU - Wang, E AU - Provenzano, M AU - Stroncek, D AU - Marincola, F M AD - National Institutes of Health, Department of Transfusion Medicine, Clinical Center, Building 10, Room 1C-711, 10 Center Drive MSC 1502, Bethesda, MD 20892-1502, USA, FMarincola@mail.cc.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 437 EP - 448 VL - 27 IS - 5 SN - 1040-8401, 1040-8401 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - Gene expression KW - Interleukin 2 KW - Toxicity KW - Tumors KW - Cancer KW - Models KW - G 07720:Immunogenetics KW - W 30925:Genetic Engineering KW - F 06915:Cancer Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20097710?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Critical+Reviews+in+Immunology&rft.atitle=Gene+Expression+Signatures+of+Interleukin-2+In+Vivo+and+In+Vitro+and+Their+Relation+to+Anticancer+Therapy&rft.au=Jin%2C+P%3BWang%2C+E%3BProvenzano%2C+M%3BStroncek%2C+D%3BMarincola%2C+F+M&rft.aulast=Jin&rft.aufirst=P&rft.date=2007-01-01&rft.volume=27&rft.issue=5&rft.spage=437&rft.isbn=&rft.btitle=&rft.title=Critical+Reviews+in+Immunology&rft.issn=10408401&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-12-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Gene expression; Interleukin 2; Tumors; Toxicity; Cancer; Models ER - TY - JOUR T1 - Analysis of Gene Coexpression by B-Spline Based CoD Estimation AN - 20064489; 9319928 AB - The gene coexpression study has emerged as a novel holistic approach for microarray data analysis. Different indices have been used in exploring coexpression relationship, but each is associated with certain pitfalls. The Pearson's correlation coefficient, for example, is not capable of uncovering nonlinear pattern and directionality of coexpression. Mutual information can detect nonlinearity but fails to show directionality. The coefficient of determination (CoD) is unique in exploring different patterns of gene coexpression, but so far only applied to discrete data and the conversion of continuous microarray data to the discrete format could lead to information loss. Here, we proposed an effective algorithm, CoexPro, for gene coexpression analysis. The new algorithm is based on B-spline approximation of coexpression between a pair of genes, followed by CoD estimation. The algorithm was justified by simulation studies and by functional semantic similarity analysis. The proposed algorithm is capable of uncovering both linear and a specific class of nonlinear relationships from continuous microarray data. It can also provide suggestions for possible directionality of coexpression to the researchers. The new algorithm presents a novel model for gene coexpression and will be a valuable tool for a variety of gene expression and network studies. The application of the algorithm was demonstrated by an analysis on ligand-receptor coexpression in cancerous and noncancerous cells. The software implementing the algorithm is available upon request to the authors. JF - Eurasip Journal on Bioinformatics and Systems Biology AU - Li, Huai AU - Sun, Yu AU - Zhan, Ming AD - Bioinformatics Unit Branch of Research Resources National Institute on Aging National Institutes of Health Baltimore MD 21224 USA, huaili@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 PB - Hindawi Publishing Corporation, P.O. Box 3079 VL - 2007 SN - 1687-4145, 1687-4145 KW - Biotechnology and Bioengineering Abstracts UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20064489?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Eurasip+Journal+on+Bioinformatics+and+Systems+Biology&rft.atitle=Analysis+of+Gene+Coexpression+by+B-Spline+Based+CoD+Estimation&rft.au=Li%2C+Huai%3BSun%2C+Yu%3BZhan%2C+Ming&rft.aulast=Li&rft.aufirst=Huai&rft.date=2007-01-01&rft.volume=2007&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Eurasip+Journal+on+Bioinformatics+and+Systems+Biology&rft.issn=16874145&rft_id=info:doi/10.1155%2F2007%2F49478 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2011-12-14 DO - http://dx.doi.org/10.1155/2007/49478 ER - TY - JOUR T1 - Protein Secretion in Gram-Negative Bacteria Via the Autotransporter Pathway AN - 20028766; 8190385 AB - Autotransporters are a large and diverse superfamily of proteins produced by pathogenic gram-negative bacteria that are composed of an N-terminal passenger domain, which typically harbors a virulence function, and a C- terminal beta domain. It has long been known that the beta domain anchors the protein to the outer membrane and facilitates transport of the passenger domain into the extracellular space. Despite the apparent simplicity of the autotransporter pathway, several aspects of autotransporter biogenesis remain poorly understood, most notably the mechanism by which the passenger domain is translocated across the outer membrane. Here we review recent evidence that the enormous sequence diversity of both passenger and beta domains belies a remarkable conservation of structure. We also discuss insights into each stage of autotransporter biogenesis that have emerged from recent structural, biochemical, and imaging studies. JF - Annual Review of Microbiology AU - Dautin, Nathalie AU - Bernstein, Harris D AD - Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0538, nathalied@niddk.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 89 EP - 112 PB - Annual Reviews, Inc., 4139 El Camino Way Box 10139 Palo Alto CA 94303-0139 USA, [mailto:service@annualreviews.org], [URL:http://annualreviews.org] VL - 61 SN - 0066-4227, 0066-4227 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology KW - Virulence KW - Reviews KW - Gram-negative bacteria KW - Secretion KW - Outer membranes KW - Conserved sequence KW - imaging KW - J 02330:Biochemistry KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20028766?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annual+Review+of+Microbiology&rft.atitle=Protein+Secretion+in+Gram-Negative+Bacteria+Via+the+Autotransporter+Pathway&rft.au=Dautin%2C+Nathalie%3BBernstein%2C+Harris+D&rft.aulast=Dautin&rft.aufirst=Nathalie&rft.date=2007-01-01&rft.volume=61&rft.issue=&rft.spage=89&rft.isbn=&rft.btitle=&rft.title=Annual+Review+of+Microbiology&rft.issn=00664227&rft_id=info:doi/10.1146%2Fannurev.micro.61.080706.093233 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Virulence; Secretion; Gram-negative bacteria; Reviews; Outer membranes; Conserved sequence; imaging DO - http://dx.doi.org/10.1146/annurev.micro.61.080706.093233 ER - TY - JOUR T1 - Probe Diffusion in Aqueous Poly(vinyl alcohol) Solutions Studied by Fluorescence Correlation Spectroscopy AN - 20023239; 8018748 AB - We report fluorescence correlation spectroscopy measurements of the translational diffusion coefficient of various probe particles in dilute and semidilute aqueous poly(vinyl alcohol) solutions. The range of sizes of the particles (fluorescent molecules, proteins, and polymers) was chosen to explore various length scales of the polymer solutions as defined by the polymer-polymer correlation length. For particles larger than the correlation length, we find that the diffusion coefficient, D, decreases exponentially with the polymer concentration. This can be explained by an exponential increase in the solution viscosity, consistent with the Stokes- Einstein equation. For probes on the order of the correlation length, the decrease of the diffusion coefficient cannot be accounted for by the Stokes- Einstein equation, but can be fit by a stretched exponential, D similar to exp(- alphac super(n)), where we find n = 0.73-0.84 and alpha is related to the probe size. These results are in accord with a diffusion model of Langevin and Rondelez (Polymer 1978, 19, 1875), where these values of n indicate a good solvent quality. JF - Biomacromolecules AU - Michelman-Ribeiro, Ariel AU - Horkay, Ferenc AU - Nossal, Ralph AU - Boukari, Hacene AD - Laboratory of Integrative and Medical Biophysics, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 1595 EP - 1600 PB - American Chemical Society, P.O. Box 182426 Columbus OH 43218-2426 USA, [mailto:service@acs.org] VL - 8 IS - 5 SN - 1525-7797, 1525-7797 KW - Biotechnology and Bioengineering Abstracts KW - Translation KW - fluorescence spectroscopy KW - Mathematical models KW - Viscosity KW - alcohols KW - Probes KW - Solvents KW - Fluorescent indicators KW - Diffusion coefficient KW - Models KW - W 30935:Food Biotechnology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20023239?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomacromolecules&rft.atitle=Probe+Diffusion+in+Aqueous+Poly%28vinyl+alcohol%29+Solutions+Studied+by+Fluorescence+Correlation+Spectroscopy&rft.au=Michelman-Ribeiro%2C+Ariel%3BHorkay%2C+Ferenc%3BNossal%2C+Ralph%3BBoukari%2C+Hacene&rft.aulast=Michelman-Ribeiro&rft.aufirst=Ariel&rft.date=2007-01-01&rft.volume=8&rft.issue=5&rft.spage=1595&rft.isbn=&rft.btitle=&rft.title=Biomacromolecules&rft.issn=15257797&rft_id=info:doi/10.1021%2Fbm061195rPII%3AS1525-7797%2806%2901195-0 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Translation; fluorescence spectroscopy; Viscosity; Mathematical models; Solvents; Probes; alcohols; Fluorescent indicators; Diffusion coefficient; Models DO - http://dx.doi.org/10.1021/bm061195rPII:S1525-7797(06)01195-0 ER - TY - JOUR T1 - Using Prior Knowledge in the Determination of Macromolecular Size- Distributions by Analytical Ultracentrifugation AN - 20013008; 7988864 AB - Analytical ultracentrifugation has reemerged as a widely used tool for the study of ensembles of biological macromolecules to understand, for example, their size-distribution and interactions in free solution. Such information can be obtained from the mathematical analysis of the concentration and signal gradients across the solution column and their evolution in time generated as a result of the gravitational force. In sedimentation velocity analytical ultracentrifugation, this analysis is frequently conducted using high resolution, diffusion-deconvoluted sedimentation coefficient distributions. They are based on Fredholm integral equations, which are ill-posed unless stabilized by regularization. In many fields, maximum entropy and Tikhonov-Phillips regularization are well- established and powerful approaches that calculate the most parsimonious distribution consistent with the data and prior knowledge, in accordance with Occam's razor. In the implementations available in analytical ultracentrifugation, to date, the basic assumption implied is that all sedimentation coefficients are equally likely and that the information retrieved should be condensed to the least amount possible. Frequently, however, more detailed distributions would be warranted by specific detailed prior knowledge on the macromolecular ensemble under study, such as the expectation of the sample to be monodisperse or paucidisperse or the expectation for the migration to establish a bimodal sedimentation pattern based on Gilbert-Jenkins' theory for the migration of chemically reacting systems. So far, such prior knowledge has remained largely unused in the calculation of the sedimentation coefficient or molecular weight distributions or was only applied as constraints. In the present paper, we examine how prior expectations can be built directly into the computational data analysis, conservatively in a way that honors the complete information of the experimental data, whether or not consistent with the prior expectation. Consistent with analogous results in other fields, we find that the use of available prior knowledge can have a dramatic effect on the resulting molecular weight, sedimentation coefficient, and size-and-shape distributions and can significantly increase both their sensitivity and their resolution. Further, the use of multiple alternative prior information allows us to probe the range of possible interpretations consistent with the data. JF - Biomacromolecules AU - Brown, Patrick H AU - Balbo, Andrea AU - Schuck, Peter AD - Protein Biophysics Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 2011 EP - 2024 PB - American Chemical Society, P.O. Box 182426 Columbus OH 43218-2426 USA, [mailto:service@acs.org] VL - 8 IS - 6 SN - 1525-7797, 1525-7797 KW - Biotechnology and Bioengineering Abstracts KW - Macromolecules KW - Mathematical models KW - Data processing KW - Gravity KW - Probes KW - Velocity KW - Computer applications KW - Migration KW - Ultracentrifugation KW - Information processing KW - Molecular weight KW - Sedimentation KW - Entropy KW - Evolution KW - W 30950:Waste Treatment & Pollution Clean-up UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20013008?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biomacromolecules&rft.atitle=Using+Prior+Knowledge+in+the+Determination+of+Macromolecular+Size-+Distributions+by+Analytical+Ultracentrifugation&rft.au=Brown%2C+Patrick+H%3BBalbo%2C+Andrea%3BSchuck%2C+Peter&rft.aulast=Brown&rft.aufirst=Patrick&rft.date=2007-01-01&rft.volume=8&rft.issue=6&rft.spage=2011&rft.isbn=&rft.btitle=&rft.title=Biomacromolecules&rft.issn=15257797&rft_id=info:doi/10.1021%2Fbm070193j LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-06-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Macromolecules; Data processing; Mathematical models; Gravity; Probes; Velocity; Computer applications; Ultracentrifugation; Migration; Molecular weight; Information processing; Sedimentation; Evolution; Entropy DO - http://dx.doi.org/10.1021/bm070193j ER - TY - JOUR T1 - Bacteriophage Mu C protein is a new member of unusual leucine zipper-HTH class of proteins AN - 20002430; 7297176 AB - Transcription activator protein C of bacteriophage Mu activates transcription of the late genes, including mom, during the lytic cycle of the phage. C binding to its site leads to the alteration in DNA topology of the promoter elements resulting in RNA polymerase (RNAP) recruitment. At the next step, the transactivator enhances promoter clearance of RNAP from P sub(mom). The C protein binds DNA with a very high affinity using a carboxyl-terminal helix turn helix (HTH) motif which has similarity with the HTH from paired domain of Drosophila prd protein. Previous studies established that the protein is dimeric in free and DNA bound forms. We describe now the unique dimerization interface of the protein. Two heptad repeats of hydrophobic amino acids found in the protein were considered to be the candidates for dimerization region. Site-directed mutational analysis revealed that the amino-terminal coiled coil region is not the dimerization determinant. In contrast, similar mutagenesis studies indicated a role for the leucine zipper motif, located in the middle region of the protein, in dimerization. Mixed oligomerization assays confirmed the importance of leucine zipper in C dimer formation establishing the presence of an uncommon zipper-HTH domain in the transactivator. JF - Protein Engineering Design and Selection AU - Chakraborty, Atanu AU - Paul, Bindu Diana AU - Nagaraja, Valakunja AD - Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India. Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892, USA. Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064, India Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1 EP - 5 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 20 IS - 1 SN - 1741-0126, 1741-0126 KW - Entomology Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts B: Bacteriology KW - Phages KW - Bacteria KW - Amino acids KW - protein C KW - Protein engineering KW - Oligomerization KW - Recruitment KW - Hydrophobicity KW - Mutagenesis KW - Promoters KW - DNA-directed RNA polymerase KW - Transcription factors KW - DNA KW - Leucine KW - C protein KW - double prime C protein KW - Leucine zipper proteins KW - Drosophila KW - W 30925:Genetic Engineering KW - Z 05300:General KW - J 02430:Symbiosis, Antibiosis & Phages KW - V 22310:Genetics, Taxonomy & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20002430?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Engineering+Design+and+Selection&rft.atitle=Bacteriophage+Mu+C+protein+is+a+new+member+of+unusual+leucine+zipper-HTH+class+of+proteins&rft.au=Chakraborty%2C+Atanu%3BPaul%2C+Bindu+Diana%3BNagaraja%2C+Valakunja&rft.aulast=Chakraborty&rft.aufirst=Atanu&rft.date=2007-01-01&rft.volume=20&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Protein+Engineering+Design+and+Selection&rft.issn=17410126&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Phages; Amino acids; Protein engineering; protein C; Recruitment; Oligomerization; Hydrophobicity; Mutagenesis; Promoters; DNA-directed RNA polymerase; Transcription factors; DNA; double prime C protein; C protein; Leucine; Leucine zipper proteins; Bacteria; Drosophila ER - TY - JOUR T1 - Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes AN - 20001942; 7261451 AB - Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes is not known. Herein, comparative genomics and experimental analyses revealed that the mammalian Sec synthase (SecS) is the previously identified pyridoxal phosphate-containing protein known as the soluble liver antigen. SecS required selenophosphate and O-phosphoseryl- tRNA super([Ser]Sec) as substrates to generate selenocysteyl-tRNA super([Ser]Sec). Moreover, it was found that Sec was synthesized on the tRNA scaffold from selenide, ATP, and serine using tRNA super([Ser]Sec), seryl-tRNA synthetase, O- phosphoseryl-tRNA super([Ser]Sec) kinase, selenophosphate synthetase, and SecS. By identifying the pathway of Sec biosynthesis in mammals, this study not only functionally characterized SecS but also assigned the function of the O- phosphoseryl-tRNA super([Ser]Sec) kinase. In addition, we found that selenophosphate synthetase 2 could synthesize monoselenophosphate in vitro but selenophosphate synthetase 1 could not. Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins. JF - PLoS Biology AU - Xu, Xue-Ming AU - Carlson, Bradley A AU - Mix, Heiko AU - Zhang, Yan AU - Saira, Kazima AU - Glass, Richard S AU - Berry, Marla J AU - Gladyshev, Vadim N AU - Hatfield, Dolph L AD - Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America Y1 - 2007 PY - 2007 DA - 2007 PB - Public Library of Science, 185 Berry Street Suite 1300 San Francisco CA 94107 USA, [mailto:plos@plos.org], [URL:http://www.plos.org] VL - 5 IS - 1 SN - 1544-9173, 1544-9173 KW - Sustainability Science Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - mammals KW - Biosynthesis KW - tRNA Sec KW - Amino acids KW - selenoproteins KW - Archaea KW - tRNA KW - selenophosphate KW - Selenocysteine KW - ATP KW - Serine-tRNA ligase KW - scaffolds KW - Liver KW - Codons KW - Proteins KW - Conservation KW - selenide KW - selenophosphate synthetase 2 KW - genomics KW - Serine KW - Genetic code KW - M3 1010:Issues in Sustainable Development KW - N 14830:RNA UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/20001942?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Assamodule&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=PLoS+Biology&rft.atitle=Biosynthesis+of+Selenocysteine+on+Its+tRNA+in+Eukaryotes&rft.au=Xu%2C+Xue-Ming%3BCarlson%2C+Bradley+A%3BMix%2C+Heiko%3BZhang%2C+Yan%3BSaira%2C+Kazima%3BGlass%2C+Richard+S%3BBerry%2C+Marla+J%3BGladyshev%2C+Vadim+N%3BHatfield%2C+Dolph+L&rft.aulast=Xu&rft.aufirst=Xue-Ming&rft.date=2007-01-01&rft.volume=5&rft.issue=1&rft.spage=&rft.isbn=&rft.btitle=&rft.title=PLoS+Biology&rft.issn=15449173&rft_id=info:doi/10.1371%2Fjournal.pbio.0050004 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - tRNA Sec; selenoproteins; Amino acids; tRNA; selenophosphate; Selenocysteine; ATP; Serine-tRNA ligase; scaffolds; Codons; Liver; selenophosphate synthetase 2; selenide; genomics; Serine; Genetic code; mammals; Biosynthesis; Conservation; Proteins; Archaea DO - http://dx.doi.org/10.1371/journal.pbio.0050004 ER - TY - JOUR T1 - The triphosphate of beta -d-4-C-ethynyl-2,3-dideoxycytidine is the preferred enantiomer substrate for HIV reverse transcriptase AN - 19997915; 7140148 AB - The enantioselective synthesis of the beta -d (1) enantiomer of 4-C- ethynyl-2,3-dideoxycytidine confirms an earlier stereochemical assignment that was strictly based on the ability of HIV reverse transcriptase and its M184V mutant to discriminate between the d- and l-configuration of nucleoside 5- triphosphates. JF - Bioorganic and Medicinal Chemistry AU - Siddiqui, Maqbool A AU - Marquez, Victor E AD - Laboratory of Medicinal Chemistry, Center for Cancer Research, NCI- Frederick, NIH, Frederick, MD 21702, USA, marquezv@dc37a.nci.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 283 EP - 287 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 15 IS - 1 SN - 0968-0896, 0968-0896 KW - Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts A: Industrial & Applied Microbiology KW - 4-C-ethynyldideoxynucleosides KW - Enantioselective synthesis KW - HIV reverse transcriptase KW - Enantiomers KW - Human immunodeficiency virus KW - nucleosides KW - RNA-directed DNA polymerase KW - A 01340:Antibiotics & Antimicrobials KW - V 22360:AIDS and HIV KW - W 30965:Miscellaneous, Reviews UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19997915?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioorganic+and+Medicinal+Chemistry&rft.atitle=The+triphosphate+of+beta+-d-4-C-ethynyl-2%2C3-dideoxycytidine+is+the+preferred+enantiomer+substrate+for+HIV+reverse+transcriptase&rft.au=Siddiqui%2C+Maqbool+A%3BMarquez%2C+Victor+E&rft.aulast=Siddiqui&rft.aufirst=Maqbool&rft.date=2007-01-01&rft.volume=15&rft.issue=1&rft.spage=283&rft.isbn=&rft.btitle=&rft.title=Bioorganic+and+Medicinal+Chemistry&rft.issn=09680896&rft_id=info:doi/10.1016%2Fj.bmc.2006.09.061 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2006-12-01 N1 - Last updated - 2015-03-25 N1 - SubjectsTermNotLitGenreText - Enantiomers; nucleosides; RNA-directed DNA polymerase; Human immunodeficiency virus DO - http://dx.doi.org/10.1016/j.bmc.2006.09.061 ER - TY - JOUR T1 - Multimodal Nanoprobes for Radionuclide and Five-Color Near-Infrared Optical Lymphatic Imaging AN - 19992848; 8019273 AB - Current contrast agents generally have one function and can only be imaged in monochrome; therefore, the majority of imaging methods can only impart uniparametric information. A single nanoparticle has the potential to be loaded with multiple payloads. Such multimodality probes have the ability to be imaged by more than one imaging technique, which could compensate for the weakness or even combine the advantages of each individual modality. Furthermore, optical imaging using different optical probes enables us to achieve multicolor in vivo imaging, wherein multiple parameters can be read from a single image. To allow differentiation of multiple optical signals in vivo, each probe should have a close but different near-infrared emission. To this end, we synthesized nanoprobes with multimodal and multicolor potential, which employed a polyamidoamine dendrimer platform linked to both radionuclides and optical probes, permitting dual-modality scintigraphic and five-color near-infrared optical lymphatic imaging using a multiple- excitation spectrally resolved fluorescence imaging technique. JF - ACS Nano AU - Kobayashi, Hisataka AU - Koyama, Yoshinori AU - Barrett, Tristan AU - Hama, Yukihiro AU - Regino, Celeste AU - Shin, In AU - Jang, Beom-Su AU - Le, Nhat AU - Paik, Chang AU - Choyke, Peter AU - Urano, Yasuteru AD - Molecular Imaging Program and, Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1088, Nuclear Medicine Department, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 258 EP - 264 PB - American Chemical Society, [mailto:service@acs.org] VL - 1 IS - 4 SN - 1936-0851, 1936-0851 KW - Immunology Abstracts; Biotechnology and Bioengineering Abstracts KW - Differentiation KW - Fluorescence KW - I.R. radiation KW - polyamidoamines KW - Radioisotopes KW - Contrast media KW - Probes KW - Fluorescent indicators KW - imaging KW - nanoparticles KW - W 30910:Imaging KW - F 06910:Microorganisms & Parasites UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19992848?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=ACS+Nano&rft.atitle=Multimodal+Nanoprobes+for+Radionuclide+and+Five-Color+Near-Infrared+Optical+Lymphatic+Imaging&rft.au=Kobayashi%2C+Hisataka%3BKoyama%2C+Yoshinori%3BBarrett%2C+Tristan%3BHama%2C+Yukihiro%3BRegino%2C+Celeste%3BShin%2C+In%3BJang%2C+Beom-Su%3BLe%2C+Nhat%3BPaik%2C+Chang%3BChoyke%2C+Peter%3BUrano%2C+Yasuteru&rft.aulast=Kobayashi&rft.aufirst=Hisataka&rft.date=2007-01-01&rft.volume=1&rft.issue=4&rft.spage=258&rft.isbn=&rft.btitle=&rft.title=ACS+Nano&rft.issn=19360851&rft_id=info:doi/10.1021%2Fnn700062z LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-03-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Differentiation; I.R. radiation; Fluorescence; polyamidoamines; Probes; Contrast media; Radioisotopes; Fluorescent indicators; nanoparticles; imaging DO - http://dx.doi.org/10.1021/nn700062z ER - TY - JOUR T1 - Use of Quantum Dot Luminescent Probes To Achieve Single-Cell Resolution of Human Oral Bacteria in Biofilms AN - 19973948; 7248913 AB - Oral biofilms are multispecies communities, and in their nascent stages of development, numerous bacterial species engage in interspecies interactions. Better insight into the spatial relationship between different species and how species diversity increases over time can guide our understanding of the role of interspecies interactions in the development of the biofilms. Quantum dots (QD) are semiconductor nanocrystals and have emerged as a promising tool for labeling and detection of bacteria. We sought to apply QD-based primary immunofluorescence for labeling of bacterial cells with in vitro and in vivo biofilms and to compare this approach with the fluorophore-based primary immunofluorescence approach we have used previously. To investigate QD-based primary immunofluorescence as the means to detect distinct targets with single-cell resolution, we conjugated polyclonal and monoclonal antibodies to the QD surface. We also conducted simultaneous QD conjugate-based and fluorophore conjugate-based immunofluorescence and showed that these conjugates were complementary tools in immunofluorescence applications. Planktonic and biofilm cells were labeled effectively by considering two factors: the final nanomolar concentration of QD conjugate and the amount of antibody conjugated to the QD, which we define as the degree of labeling. These advances in the application of QD-based immunofluorescence for the study of biofilms in vitro and in vivo will help to define bacterial community architecture and to facilitate investigations of interactions between bacterial species in these communities. JF - Applied and Environmental Microbiology AU - Chalmers, Natalia I AU - Palmer, Robert JJr AU - Du-Thumm, Laurence AU - Sullivan, Richard AU - Shi, Wenyuan AU - Kolenbrander, Paul E AD - Department of Biomedical Sciences, University of Maryland Dental School, Baltimore, Maryland 21201. National Institute of Dental and Craniofacial Research, National Institutes of Health, Building 30, Room 310, Bethesda, Maryland 20892. Colgate-Palmolive Company, Piscataway, New Jersey 08855. Department of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, California 90095. UCLA Molecular Biology Institute, Los Angeles, California 90025 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 630 EP - 636 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 73 IS - 2 SN - 0099-2240, 0099-2240 KW - Microbiology Abstracts B: Bacteriology; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts A: Industrial & Applied Microbiology KW - Monoclonal antibodies KW - Quantum dots KW - Species diversity KW - Probes KW - Developmental stages KW - Immunofluorescence KW - Biofilms KW - fluorophores KW - J 02350:Immunology KW - W 30900:Methods KW - A 01300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19973948?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Applied+and+Environmental+Microbiology&rft.atitle=Use+of+Quantum+Dot+Luminescent+Probes+To+Achieve+Single-Cell+Resolution+of+Human+Oral+Bacteria+in+Biofilms&rft.au=Chalmers%2C+Natalia+I%3BPalmer%2C+Robert+JJr%3BDu-Thumm%2C+Laurence%3BSullivan%2C+Richard%3BShi%2C+Wenyuan%3BKolenbrander%2C+Paul+E&rft.aulast=Chalmers&rft.aufirst=Natalia&rft.date=2007-01-01&rft.volume=73&rft.issue=2&rft.spage=630&rft.isbn=&rft.btitle=&rft.title=Applied+and+Environmental+Microbiology&rft.issn=00992240&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Quantum dots; Monoclonal antibodies; Species diversity; Probes; Developmental stages; fluorophores; Biofilms; Immunofluorescence ER - TY - JOUR T1 - Stage-specific alterations of the genome, transcriptome, and proteome during colorectal carcinogenesis AN - 19887059; 7913206 AB - To identify sequential alterations of the genome, transcriptome, and proteome during colorectal cancer progression, we have analyzed tissue samples from 36 patients, including the complete mucosa-adenoma-carcinoma sequence from 8 patients. Comparative genomic hybridization (CGH) revealed patterns of stage specific, recurrent genomic imbalances. Gene expression analysis on 9K cDNA arrays identified 58 genes differentially expressed between normal mucosa and adenoma, 116 genes between adenoma and carcinoma, and 158 genes between primary carcinoma and liver metastasis (P < 0.001). Parallel analysis of our samples by CGH and expression profiling revealed a direct correlation of chromosomal copy number changes with chromosome-specific average gene expression levels. Protein expression was analyzed by two-dimensional gel electrophoresis and subsequent mass spectrometry. Although there was no direct match of differentially expressed proteins and genes, the majority of them belonged to identical pathways or networks. In conclusion, increasing genomic instability and a recurrent pattern of chromosomal imbalances as well as specific gene and protein expression changes correlate with distinct stages of colorectal cancer progression. Chromosomal aneuploidies directly affect average resident gene expression levels, thereby contributing to a massive deregulation of the cellular transcriptome. The identification of novel genes and proteins might deliver molecular targets for diagnostic and therapeutic interventions. JF - Genes, Chromosomes and Cancer AU - Habermann, Jens K AU - Paulsen, Ulrike AU - Roblick, Uwe J AU - Upender, Madhvi B AU - McShane, Lisa M AU - Korn, Edward L AU - Wangsa, Danny AU - Kruger, Stefan AU - Duchrow, Michael AU - Bruch, Hans-Peter AU - Auer, Gert AU - Ried, Thomas AD - Genetics Branch, National Cancer Institute, NIH, Bethesda, MD, habermaj@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 10 EP - 26 PB - John Wiley & Sons, 111 River Street Hoboken NJ 07030 USA, [mailto:custserv@wiley.com], [URL:http://www.wiley.com/] VL - 46 IS - 1 SN - 1045-2257, 1045-2257 KW - Toxicology Abstracts; Genetics Abstracts KW - Genomes KW - Aneuploidy KW - Nucleotide sequence KW - Mucosa KW - Colorectal cancer KW - Therapeutic applications KW - DNA microarrays KW - Gel electrophoresis KW - Mass spectroscopy KW - copy number KW - Carcinoma KW - Metastases KW - Gene expression KW - Genomic instability KW - Carcinogenesis KW - Liver KW - Adenoma KW - G 07880:Human Genetics KW - X 24310:Pharmaceuticals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19887059?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Genes%2C+Chromosomes+and+Cancer&rft.atitle=Stage-specific+alterations+of+the+genome%2C+transcriptome%2C+and+proteome+during+colorectal+carcinogenesis&rft.au=Habermann%2C+Jens+K%3BPaulsen%2C+Ulrike%3BRoblick%2C+Uwe+J%3BUpender%2C+Madhvi+B%3BMcShane%2C+Lisa+M%3BKorn%2C+Edward+L%3BWangsa%2C+Danny%3BKruger%2C+Stefan%3BDuchrow%2C+Michael%3BBruch%2C+Hans-Peter%3BAuer%2C+Gert%3BRied%2C+Thomas&rft.aulast=Habermann&rft.aufirst=Jens&rft.date=2007-01-01&rft.volume=46&rft.issue=1&rft.spage=10&rft.isbn=&rft.btitle=&rft.title=Genes%2C+Chromosomes+and+Cancer&rft.issn=10452257&rft_id=info:doi/10.1002%2Fgcc.20382 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-01-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Genomes; Aneuploidy; Nucleotide sequence; Mucosa; Colorectal cancer; Therapeutic applications; DNA microarrays; Mass spectroscopy; Gel electrophoresis; Carcinoma; copy number; Gene expression; Metastases; Genomic instability; Carcinogenesis; Liver; Adenoma DO - http://dx.doi.org/10.1002/gcc.20382 ER - TY - JOUR T1 - Overview of the Molecular Carcinogenesis of Mouse Lung Tumor Models of Human Lung Cancer AN - 19878443; 7692027 AB - Lung cancer is the leading cause of cancer death worldwide, and the need to develop better diagnostic techniques and therapies is urgent. Mouse models have been utilized for studying carcinogenesis of human lung cancers, and many of the major genetic alterations detected in human lung cancers have also been identified in mouse lung tumors. The importance of mouse models for understanding human lung carcinogenic processes and in developing early diagnostic techniques, preventive measures and therapies cannot be overstated. In this report, the major known molecular alterations in lung tumorigenesis of mice are reviewed and compared to those in humans. JF - Toxicologic Pathology AU - Wakamatsu, Nobuko AU - Devereux, Theodora R AU - Hong, Hue-Hua L AU - Sills, Robert C AD - Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 75 EP - 80 PB - Taylor & Francis, 11 New Fetter Lane London EC4P 4EE UK, [mailto:info@tandf.co.uk], [URL:http://www.tandf.co.uk] VL - 35 IS - 1 SN - 0192-6233, 0192-6233 KW - Toxicology Abstracts KW - Molecular modelling KW - Reviews KW - Tumorigenesis KW - Carcinogenesis KW - Animal models KW - Lung cancer KW - X 24300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19878443?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicologic+Pathology&rft.atitle=Overview+of+the+Molecular+Carcinogenesis+of+Mouse+Lung+Tumor+Models+of+Human+Lung+Cancer&rft.au=Wakamatsu%2C+Nobuko%3BDevereux%2C+Theodora+R%3BHong%2C+Hue-Hua+L%3BSills%2C+Robert+C&rft.aulast=Wakamatsu&rft.aufirst=Nobuko&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=75&rft.isbn=&rft.btitle=&rft.title=Toxicologic+Pathology&rft.issn=01926233&rft_id=info:doi/10.1080%2F01926230601059993 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Molecular modelling; Reviews; Carcinogenesis; Tumorigenesis; Animal models; Lung cancer DO - http://dx.doi.org/10.1080/01926230601059993 ER - TY - JOUR T1 - Evaluation of combination gene therapy with PTEN and antisense hTERT for malignant glioma in vitro and xenografts AN - 19876406; 7400045 AB - Telomerase activation is a critical event in cell immortalization, and an increase in human telomerase reverse transcriptase (hTERT) expression is the key step in activating telomerase. The phosphatase and tensin homolog (PTEN) gene encodes a double-specific phosphatase that induces cell cycle arrest, inhibits cell growth, and causes apoptotic cell death. Here, we evaluated a combined PTEN and antisense hTERT gene therapy for experimental glioma in vitro and in vivo. We demonstrated that infection with antisense-hTERT and wild-type-PTEN adenoviruses significantly inhibited human U251 glioma cell proliferation in vitro and glioma growth in a xenograft mouse model. The efficacy of therapy was obviously higher in the tumor xenografts infected with both PTEN and antisense hTERT than in the gliomas infected with either agent alone at the same total viral dose. Consistent with these results, we showed that telomerase activity and hTERT protein levels were markedly reduced in the glioma cells following adenovirus infection. In contrast, the levels of PTEN protein expression were dramatically increased in these cells. Our data indicate that combination treatment with antisense hTERT and wild-type PTEN effectively suppresses the malignant growth of human glioma cells in vitro and in tumor xenografts, suggesting a promising new approach in glioma gene therapy that warrants further investigation. JF - Cellular and Molecular Life Sciences AU - You, Y AU - Geng, X AU - Zhao, P AU - Fu, Z AU - Wang, C AU - Chao, S AU - Liu, N AU - Lu, A AU - Gardner, K AU - Pu, P AU - Kong, C AU - Ge, Y AU - Judge, SIV AU - Li, Q Q AD - Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (USA), liquenti@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 621 EP - 631 VL - 64 IS - 5 SN - 1420-682X, 1420-682X KW - Genetics Abstracts; Virology & AIDS Abstracts; Biotechnology and Bioengineering Abstracts KW - Cell immortalization KW - Apoptosis KW - Data processing KW - Gene therapy KW - Cell cycle KW - Adenovirus KW - Animal models KW - Tumors KW - telomerase reverse transcriptase KW - PTEN protein KW - Infection KW - Brain tumors KW - Cell death KW - Antisense KW - Glioma cells KW - Xenografts KW - Glioma KW - Cell proliferation KW - W 30905:Medical Applications KW - V 22300:Methods KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19876406?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cellular+and+Molecular+Life+Sciences&rft.atitle=Evaluation+of+combination+gene+therapy+with+PTEN+and+antisense+hTERT+for+malignant+glioma+in+vitro+and+xenografts&rft.au=You%2C+Y%3BGeng%2C+X%3BZhao%2C+P%3BFu%2C+Z%3BWang%2C+C%3BChao%2C+S%3BLiu%2C+N%3BLu%2C+A%3BGardner%2C+K%3BPu%2C+P%3BKong%2C+C%3BGe%2C+Y%3BJudge%2C+SIV%3BLi%2C+Q+Q&rft.aulast=You&rft.aufirst=Y&rft.date=2007-01-01&rft.volume=64&rft.issue=5&rft.spage=621&rft.isbn=&rft.btitle=&rft.title=Cellular+and+Molecular+Life+Sciences&rft.issn=1420682X&rft_id=info:doi/10.1007%2Fs00018-007-6424-4 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Cell immortalization; Data processing; Apoptosis; Gene therapy; Cell cycle; Animal models; PTEN protein; telomerase reverse transcriptase; Tumors; Infection; Brain tumors; Antisense; Cell death; Glioma cells; Glioma; Xenografts; Cell proliferation; Adenovirus DO - http://dx.doi.org/10.1007/s00018-007-6424-4 ER - TY - JOUR T1 - Exploring SCC-DFTB Paths for Mapping QM/MM Reaction Mechanisms AN - 19859423; 8113741 AB - A new first-order procedure for locating transition structures (TS) that employs hybrid quantum mechanical/molecular mechanical (QM/MM) potentials has been developed. This new technique (RPATh+RESD) combines the replica path method (RPATh) and standard reaction coordinate driving (RCD) techniques in an approach that both efficiently determines reaction barriers and successfully eliminates two key weaknesses of RCD calculations (i.e., hysteresis/discontinuities in the path and the sequential nature of the RCD procedure). In addition, we have extended CHARMM's QM/MM reaction pathway methods, the RPATh and nudged elastic band (NEB) methods, to incorporate SCC- DFTB wave functions. This newly added functionality has been applied to the chorismate mutase-catalyzed interconversion of chorismate to prephenate, which is a key step in the shikimate pathway of bacteria, fungi, and other higher plants. The RPATh+RESD barrier height (DeltaE super(thermod) = 5.7 kcal/mol) is in good agreement with previous results from full-energy surface mapping studies (Zhang, X.; Zhang, X.; Bruice, T. C. Biochemistry 2005, 44, 10443-10448). Full reaction paths were independently mapped with RPATh and NEB methods and showed good agreement with the final transition state from the RPATh+RESD "gold standard" and previous high-level QM/MM transition states (Woodcock, H. L.; Hodoscek, M.; Gilbert, T. B.; Gill, P. M. W.; Schaefer, H. F.; Brooks, B. R. J. Comput. Chem. 2007, 28, 1485- 1502). The SCC-DFTB TS geometry most closely approximates the MP2/6-31+G(d) QM/MM result. However, the barrier height is underestimated and possibly points to an area for improvement in SCC-DFTB parametrization. In addition, the steepest descents (SD) minimizer for the NEB method was modified to uncouple the in-path and off-path degrees of freedom during the minimization, which significantly improved performance. The convergence behavior of the RPATh and NEB was examined for SCC-DFTB wave functions, and it was determined that, in general, both methods converge at about the same rate, although the techniques used for convergence may be different. For instance, RPATh can effectively use the adopted basis Newton-Raphson (ABNR) minimizer, where NEB seems to require a combination of SD and ABNR. JF - Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory AU - Woodcock, HLee AU - Hodoscek, Milan AU - Brooks, Bernard R AD - Laboratory of Computational Biology, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 5720 EP - 5728 PB - American Chemical Society, P.O. Box 182426 Columbus OH 43218-2426 USA, [mailto:service@acs.org] VL - 111 IS - 26 SN - 1089-5639, 1089-5639 KW - Microbiology Abstracts C: Algology, Mycology & Protozoology; Microbiology Abstracts B: Bacteriology KW - Reaction mechanisms KW - Convergence KW - Kinetics KW - Fungi KW - Hybrids KW - Waves KW - Hysteresis KW - Mapping KW - Spectroscopy KW - Gills KW - K 03300:Methods KW - J 02300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19859423?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Physical+Chemistry+A%3A+Molecules%2C+Spectroscopy%2C+Kinetics%2C+Environment+and+General+Theory&rft.atitle=Exploring+SCC-DFTB+Paths+for+Mapping+QM%2FMM+Reaction+Mechanisms&rft.au=Woodcock%2C+HLee%3BHodoscek%2C+Milan%3BBrooks%2C+Bernard+R&rft.aulast=Woodcock&rft.aufirst=HLee&rft.date=2007-01-01&rft.volume=111&rft.issue=26&rft.spage=5720&rft.isbn=&rft.btitle=&rft.title=Journal+of+Physical+Chemistry+A%3A+Molecules%2C+Spectroscopy%2C+Kinetics%2C+Environment+and+General+Theory&rft.issn=10895639&rft_id=info:doi/10.1021%2Fjp0714217PII%3AS1089-5639%2807%2901421-1 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Reaction mechanisms; Convergence; Hybrids; Fungi; Kinetics; Hysteresis; Waves; Mapping; Spectroscopy; Gills DO - http://dx.doi.org/10.1021/jp0714217PII:S1089-5639(07)01421-1 ER - TY - JOUR T1 - Entrez Gene: gene-centered information at NCBI AN - 19852367; 7254413 AB - Entrez Gene (www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene) is NCBI's database for gene-specific information. Entrez Gene includes records from genomes that have been completely sequenced, that have an active research community to contribute gene-specific information or that are scheduled for intense sequence analysis. The content of Entrez Gene represents the result of both curation and automated integration of data from NCBI's Reference Sequence project (RefSeq), from collaborating model organism databases and from other databases within NCBI. Records in Entrez Gene are assigned unique, stable and tracked integers as identifiers. The content (nomenclature, map location, gene products and their attributes, markers, phenotypes and links to citations, sequences, variation details, maps, expression, homologs, protein domains and external databases) is provided via interactive browsing through NCBI's Entrez system, via NCBI's Entrez programing utilities (E-Utilities), and for bulk transfer by ftp. JF - Nucleic Acids Research AU - Maglott, Donna AU - Ostell, Jim AU - Pruitt, Kim D AU - Tatusova, Tatiana AD - National Center for Biotechnology Information, National Library of Medicine National Institutes of Health, Bethesda, MD 20892-6510, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D26 EP - D31 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Databases KW - Integration KW - Data processing KW - Browsing KW - Gene mapping KW - Models KW - N 14845:Miscellaneous KW - G 07870:Mammals KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19852367?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=Entrez+Gene%3A+gene-centered+information+at+NCBI&rft.au=Maglott%2C+Donna%3BOstell%2C+Jim%3BPruitt%2C+Kim+D%3BTatusova%2C+Tatiana&rft.aulast=Maglott&rft.aufirst=Donna&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D26&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Genomes; Integration; Databases; Data processing; Browsing; Models; Gene mapping ER - TY - JOUR T1 - RegTransBase-a database of regulatory sequences and interactions in a wide range of prokaryotic genomes AN - 19850378; 7254444 AB - RegTransBase is a manually curated database of regulatory interactions in prokaryotes that captures the knowledge in public scientific literature using a controlled vocabulary. Although several databases describing interactions between regulatory proteins and their binding sites are already being maintained, they either focus mostly on the model organisms Escherichia coli and Bacillus subtilis or are entirely computationally derived. RegTransBase describes a large number of regulatory interactions reported in many organisms and contains the following types of experimental data: the activation or repression of transcription by an identified direct regulator, determining the transcriptional regulatory function of a protein (or RNA) directly binding to DNA (RNA), mapping or prediction of a binding site for a regulatory protein and characterization of regulatory mutations. Currently, RegTransBase content is derived from about 3000 relevant articles describing over 7000 experiments in relation to 128 microbes. It contains data on the regulation of about 7500 genes and evidence for 6500 interactions with 650 regulators. RegTransBase also contains manually created position weight matrices (PWM) that can be used to identify candidate regulatory sites in over 60 species. RegTransBase is available at http://regtransbase.lbl.gov. JF - Nucleic Acids Research AU - Kazakov, Alexei E AU - Cipriano, Michael J AU - Novichkov, Pavel S AU - Minovitsky, Simon AU - Vinogradov, Dmitry V AU - Arkin, Adam AU - Mironov, Andrey A AU - Gelfand, Mikhail S AU - Dubchak, Inna AD - Institute for Information Transmission Problems, RAS. Bolshoi Karetny pereulok 19 Moscow, 127994, Russia. Lawrence Berkeley National Laboratory, 1 Cyclotron Road Berkeley, CA 94720, USA. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Building 38A, 8600 Rockville Pike, Bethesda, MD 20894, USA. Howard Hughes Medical Institute 4000 Jones Bridge Road Chevy Chase, MD 20815-6789, USA. Department of Bioengineering, University of California Berkeley, CA, 94710, USA. Virtual Institute of Microbial Stress and Survival, Berkeley CA, 94710, USA. Faculty of Bioengineering and Bioinformatics, Moscow State University Vorobievy Gory 1-73, Moscow 119992, Russia. State Research Center GosNIIGenetika. 1-j Dorozhny proezd 1 Moscow, 117545, Russia. Department of Energy Joint Genome Institute 2800 Mitchell Drive,Walnut Creek, CA 94598, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D407 EP - D412 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Genetics Abstracts; Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Bacillus subtilis KW - Data processing KW - Peptide mapping KW - Regulatory sequences KW - Transcription KW - Models KW - Databases KW - regulatory proteins KW - RNA KW - Escherichia coli KW - DNA KW - Prokaryotes KW - Mutation KW - Gene mapping KW - Gene silencing KW - J 02310:Genetics & Taxonomy KW - N 14810:Methods KW - G 07770:Bacteria KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19850378?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=RegTransBase-a+database+of+regulatory+sequences+and+interactions+in+a+wide+range+of+prokaryotic+genomes&rft.au=Kazakov%2C+Alexei+E%3BCipriano%2C+Michael+J%3BNovichkov%2C+Pavel+S%3BMinovitsky%2C+Simon%3BVinogradov%2C+Dmitry+V%3BArkin%2C+Adam%3BMironov%2C+Andrey+A%3BGelfand%2C+Mikhail+S%3BDubchak%2C+Inna&rft.aulast=Kazakov&rft.aufirst=Alexei&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D407&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Genomes; Data processing; Peptide mapping; Regulatory sequences; Transcription; Models; Databases; RNA; regulatory proteins; DNA; Prokaryotes; Mutation; Gene silencing; Gene mapping; Bacillus subtilis; Escherichia coli ER - TY - JOUR T1 - Cleavage of a bacterial autotransporter by an evolutionarily convergent autocatalytic mechanism AN - 19847471; 7385934 AB - Bacterial autotransporters are comprised of an N-terminal 'passenger domain' and a C-terminal [beta] barrel ('[beta] domain') that facilitates transport of the passenger domain across the outer membrane. Following translocation, the passenger domains of some autotransporters are cleaved by an unknown mechanism. Here we show that the passenger domain of the Escherichia coli O157:H7 autotransporter EspP is released in a novel autoproteolytic reaction. After purification, the uncleaved EspP precursor underwent proteolytic processing in vitro. An analysis of protein topology together with mutational studies strongly suggested that the reaction occurs inside the [beta] barrel and revealed that two conserved residues, an aspartate within the [beta] domain (Asp super(1120)) and an asparagine (Asn super(1023)) at the P1 position of the cleavage junction, are essential for passenger domain cleavage. Interestingly, these residues were also essential for the proteolytic processing of two distantly related autotransporters. The data strongly suggest that Asp super(1120) and Asn super(1023) form an unusual catalytic dyad that mediates self-cleavage through the cyclization of the asparagine. Remarkably, a very similar mechanism has been proposed for the maturation of eukaryotic viral capsids. JF - EMBO Journal AU - Dautin, Nathalie AU - Barnard, Travis J AU - Anderson, D Eric AU - Bernstein, Harris D AD - Genetics and Biochemistry Branch, National Institutes of Health, Bethesda, MD, USA, harris_bernstein@nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1942 EP - 1952 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com], [URL:http://www.nature.com/] VL - 26 IS - 7 SN - 0261-4189, 0261-4189 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology KW - autotransporters KW - enzyme mechanism KW - E. coli KW - protease KW - secretion KW - Proteolysis KW - Capsids KW - Data processing KW - Outer membranes KW - Escherichia coli KW - Asparagine KW - Translocation KW - Evolution KW - J 02310:Genetics & Taxonomy KW - A 01490:Miscellaneous KW - V 22320:Replication UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19847471?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=EMBO+Journal&rft.atitle=Cleavage+of+a+bacterial+autotransporter+by+an+evolutionarily+convergent+autocatalytic+mechanism&rft.au=Dautin%2C+Nathalie%3BBarnard%2C+Travis+J%3BAnderson%2C+D+Eric%3BBernstein%2C+Harris+D&rft.aulast=Dautin&rft.aufirst=Nathalie&rft.date=2007-01-01&rft.volume=26&rft.issue=7&rft.spage=1942&rft.isbn=&rft.btitle=&rft.title=EMBO+Journal&rft.issn=02614189&rft_id=info:doi/10.1038%2Fsj.emboj.7601638 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-06-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Capsids; Proteolysis; Data processing; Outer membranes; Translocation; Asparagine; Evolution; Escherichia coli DO - http://dx.doi.org/10.1038/sj.emboj.7601638 ER - TY - JOUR T1 - Development of leiomyosarcoma of the uterus in MMTV-CR-1 transgenic mice AN - 19846473; 7185744 AB - Overexpression of Cripto-1 (CR-1) in FVB/N mice using the MMTV-LTR promoter results in increased mammary tumourigenesis in these female transgenic mice (MMTV-CR-1). Here, we characterize uterine tumours that developed in 15/76 (19.7%) of MMTV-CR-1 female nulliparous or multiparous mice during 24 months of observation compared with 0/33 (0%) of FVB/N normal control mice observed during the same time period (p < 0.01). The uterine tumours collected from the MMTV-CR-1 mice were classified as leiomyosarcomas and found to express the CR-1 transgene by polymerase chain reaction analysis and immunohistochemistry. Detection by western blot analysis showed higher levels of phosphorylated (P) forms of c-src, Akt, GSK-3 beta , and dephosphorylated (DP)- beta -catenin in lysates from MMTV-CR-1 uterine leiomyosarcomas in comparison with lysates from normal control FVB/N uteri. Immunostaining showed increased nuclear localization of beta - catenin in the MMTV-CR-1 uterine leiomyosarcomas. Increased immunostaining for CR-1 was detected in 9/13 (69.2%) cases of human leiomyosarcoma compared with staining in 3/15 (20%) human leiomyoma sections. Stronger immunostaining for P- src, P-Akt, P-GSK-3 beta and increased nuclear localization of beta -catenin was also seen in human leiomyosarcomas in comparison with leiomyomas. Normal human uterine smooth muscle (UtSM) cells treated with exogenous soluble rhCR-1 showed increased levels of P-src, P-Akt, P-GSK-3 beta and dephosphorylated (DP)- beta -catenin and increased proliferation (p < 0.05) and migration (p < 0.01) in comparison with untreated control UtSM cells. Inhibitors against c-src, Akt or beta -catenin, individually or in combination, significantly reduced CR-1-induced migration. These results suggest a role for CR-1 during uterine tumourigenesis either directly by activating c-src and Akt and/or via cross-talk with the canonical Wnt signalling pathway, as suggested by the increased expression of P- GSK-3 beta , DP- beta -catenin, and increased nuclear localization of beta -catenin in human and MMTV-CR-1 mice leiomyosarcomas. JF - Journal of Pathology AU - Strizzi, L AU - Bianco, C AU - Hirota, M AU - Watanabe, K AU - Mancino, M AU - Hamada, S AU - Raafat, A AU - Lawson, S AU - Ebert, Ad AU - DAntonio, A AU - Losito, S AU - Normanno, N AU - Salomon, Ds AD - Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, MD, USA, salomond@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 36 EP - 44 PB - John Wiley & Sons, Inc., 111 River Street Hoboken NJ 07030 USA, [mailto:custserv@wiley.com], [URL:http://www.wiley.com/] VL - 211 IS - 1 SN - 0022-3417, 0022-3417 KW - Biotechnology and Bioengineering Abstracts KW - Cripto-1 KW - transgenic mice KW - uterus KW - sarcoma KW - Smooth muscle KW - Western blotting KW - Uterus KW - Wnt protein KW - Tumorigenesis KW - Transgenic mice KW - Promoters KW - catenin KW - AKT protein KW - Src protein KW - Polymerase chain reaction KW - Cell migration KW - Cell proliferation KW - Immunohistochemistry KW - Signal transduction KW - W 30925:Genetic Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19846473?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Pathology&rft.atitle=Development+of+leiomyosarcoma+of+the+uterus+in+MMTV-CR-1+transgenic+mice&rft.au=Strizzi%2C+L%3BBianco%2C+C%3BHirota%2C+M%3BWatanabe%2C+K%3BMancino%2C+M%3BHamada%2C+S%3BRaafat%2C+A%3BLawson%2C+S%3BEbert%2C+Ad%3BDAntonio%2C+A%3BLosito%2C+S%3BNormanno%2C+N%3BSalomon%2C+Ds&rft.aulast=Strizzi&rft.aufirst=L&rft.date=2007-01-01&rft.volume=211&rft.issue=1&rft.spage=36&rft.isbn=&rft.btitle=&rft.title=Journal+of+Pathology&rft.issn=00223417&rft_id=info:doi/10.1002%2Fpath.2083 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-05-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Smooth muscle; Western blotting; Uterus; Wnt protein; Tumorigenesis; Transgenic mice; Promoters; catenin; Src protein; AKT protein; Polymerase chain reaction; Cell migration; Cell proliferation; Immunohistochemistry; Signal transduction DO - http://dx.doi.org/10.1002/path.2083 ER - TY - JOUR T1 - Effect of Neutropenia and Treatment Delay on the Response to Antifungal Agents in Experimental Disseminated Candidiasis AN - 19843439; 7205167 AB - Disseminated candidiasis is associated with a high rate of morbidity and mortality. The presence of neutrophils and the timely administration of antifungal agents are likely to be critical factors for a favorable therapeutic outcome of this syndrome. The effect of neutropenia on the temporal profile of the burden of Candida albicans in untreated mice and those treated with amphotericin B was determined using a pharmacodynamic model of disseminated candidiasis. A mathematical model was developed to describe the rate and extent of the C. albicans killing attributable to neutrophils and to amphotericin B. The consequences of a delay in the administration of amphotericin B, flucytosine, or micafungin were studied by defining dose-response relationships. Neutrophils caused a logarithmic decline in fungal burden in treated and untreated mice. The combination of amphotericin B and neutrophils resulted in a high rate of Candida killing and a sustained anti-C. albicans effect. In neutropenic mice, 5 mg/kg of body weight of amphotericin B was required to prevent progressive logarithmic growth. An increased delay in drug administration resulted in a reduction in the maximum effect to a point at which no drug effect could be observed. Neutrophils and the timely initiation of antifungal agents are critical determinants in the treatment of experimental disseminated candidiasis. JF - Antimicrobial Agents & Chemotherapy AU - Hope, William W AU - Drusano, George L AU - Moore, Caroline B AU - Sharp, Andrew AU - Louie, Arnold AU - Walsh, Thomas J AU - Denning, David W AU - Warn, Peter A AD - School of Medicine, 1.800 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom. Emerging Infections and Host Defense Section, Ordway Research Institute, Albany, New York 12208. Immunocompromised Host Section, Pediatric Oncology Branch, NCI/NIH, Bethesda, Maryland 20892. Wythenshawe Hospital, Manchester M23 9LT, United Kingdom Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 285 EP - 295 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 51 IS - 1 SN - 0066-4804, 0066-4804 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Mortality KW - Amphotericin B KW - Antifungal agents KW - Mathematical models KW - Candidiasis KW - micafungin KW - Animal models KW - Leukocytes (neutrophilic) KW - Candida albicans KW - flucytosine KW - Morbidity KW - Antimicrobial agents KW - Neutropenia KW - Body weight KW - Dose-response effects KW - Drugs KW - Pharmacodynamics KW - K 03340:Effects of Physical & Chemical Factors KW - A 01340:Antibiotics & Antimicrobials UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19843439?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologya&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Antimicrobial+Agents+%26+Chemotherapy&rft.atitle=Effect+of+Neutropenia+and+Treatment+Delay+on+the+Response+to+Antifungal+Agents+in+Experimental+Disseminated+Candidiasis&rft.au=Hope%2C+William+W%3BDrusano%2C+George+L%3BMoore%2C+Caroline+B%3BSharp%2C+Andrew%3BLouie%2C+Arnold%3BWalsh%2C+Thomas+J%3BDenning%2C+David+W%3BWarn%2C+Peter+A&rft.aulast=Hope&rft.aufirst=William&rft.date=2007-01-01&rft.volume=51&rft.issue=1&rft.spage=285&rft.isbn=&rft.btitle=&rft.title=Antimicrobial+Agents+%26+Chemotherapy&rft.issn=00664804&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Amphotericin B; Mortality; Antifungal agents; Mathematical models; Candidiasis; micafungin; Leukocytes (neutrophilic); Animal models; Morbidity; flucytosine; Antimicrobial agents; Neutropenia; Body weight; Dose-response effects; Drugs; Pharmacodynamics; Candida albicans ER - TY - JOUR T1 - NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins AN - 19841818; 7254489 AB - NCBI's reference sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) is a curated non-redundant collection of sequences representing genomes, transcripts and proteins. The database includes 3774 organisms spanning prokaryotes, eukaryotes and viruses, and has records for 2 879 860 proteins (RefSeq release 19). RefSeq records integrate information from multiple sources, when additional data are available from those sources and therefore represent a current description of the sequence and its features. Annotations include coding regions, conserved domains, tRNAs, sequence tagged sites (STS), variation, references, gene and protein product names, and database cross-references. Sequence is reviewed and features are added using a combined approach of collaboration and other input from the scientific community, prediction, propagation from GenBank and curation by NCBI staff. The format of all RefSeq records is validated, and an increasing number of tests are being applied to evaluate the quality of sequence and annotation, especially in the context of complete genomic sequence. JF - Nucleic Acids Research AU - Pruitt, Kim D AU - Tatusova, Tatiana AU - Maglott, Donna R AD - National Center for Biotechnology Information, National Library of Medicine National Institutes of Health Rm 6An.12J, 45 Center Drive, Bethesda, MD 20892-6510, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D61 EP - D65 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Genetics Abstracts; Virology & AIDS Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Databases KW - Data processing KW - Reviews KW - tRNA KW - Information processing KW - Conserved sequence KW - genomics KW - Prokaryotes KW - G 07880:Human Genetics KW - V 22320:Replication KW - N 14845:Miscellaneous KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19841818?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=NCBI+reference+sequences+%28RefSeq%29%3A+a+curated+non-redundant+sequence+database+of+genomes%2C+transcripts+and+proteins&rft.au=Pruitt%2C+Kim+D%3BTatusova%2C+Tatiana%3BMaglott%2C+Donna+R&rft.aulast=Pruitt&rft.aufirst=Kim&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D61&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Genomes; Databases; Data processing; Information processing; tRNA; Reviews; Conserved sequence; Prokaryotes; genomics ER - TY - JOUR T1 - Inhibition of estrogen-independent mammary carcinogenesis by disruption of growth hormone signaling AN - 19840058; 7206697 AB - Clinical trials and laboratory-based studies indicate that the growth hormone/insulin-like growth factor-I axis may affect the development of breast cancer. The purpose of the present investigation was to develop a genetic model of mammary cancer to test the hypothesis that downregulation of GH signaling can substantially retard mammary cancer progression. We crossed the Laron mouse, in which the gene for the GH receptor/binding protein has been disrupted, with the C3(1)/TAg mouse, which develops estrogen receptor alpha negative mammary cancers. All mice used in our experiments were heterozygous for the large T antigen (TAg) and either homozygous wild-type for GHR (Ghr super(+/+)) or null for GHR (Ghr super(-/-)). Compared with the TAg/Ghr super(+/+) mice, the TAg/Ghr super(-/-) mice showed delayed mammary cancer latency with significantly decreased multiplicity (9.8 plus or minus 1.4 versus 3.2 plus or minus 1.2) and volume (776.1 plus or minus 284.4 versus 50.5 plus or minus 8.9 mm super(3)). Furthermore, the frequency of mammary hyperplasias was significantly reduced in the TAg/Ghr super(-/-) mice (15.0 plus or minus 1.7 versus 6.8 plus or minus 1.7). To establish that these mammary cancers were estrogen-independent, 12-week-old TAg/Ghr super(+/+) mice, which lack visible hyperplasia, were either ovariectomized (ovx) or sham operated (sham). Compared with the sham group, ovariectomy resulted in no difference in the frequency of mammary hyperplasia, mammary tumor latency, incidence, multiplicity or tumor size. Together, these data demonstrate that the disruption of GH signaling significantly retards TAg-driven mammary carcinogenesis, and suggest that disrupting GH signaling may be an effective strategy to inhibit the progression of estrogen-independent breast cancer. JF - Carcinogenesis AU - Zhang, Xiao AU - Mehta, Rajendra G AU - Lantvit, Daniel D AU - Coschigano, Karen T AU - Kopchick, John J AU - Green, Jeffrey E AU - Hedayat, Samad AU - Christov, Konstantin T AU - Ray, Vera H AU - Unterman, Terry G AU - Swanson, Steven M AD - Department of Medicinal Chemistry and Pharmacognosy University of Illinois at Chicago, Chicago, IL 60612. Department of Surgical Oncology University of Illinois at Chicago, Chicago, IL 60612. Department of Mathematics, Statistics and Computer Science University of Illinois at Chicago, Chicago, IL 60612. Department of Medicine University of Illinois at Chicago, Chicago, IL 60612. Department of Veterans Affairs Jesse Brown Medical Center, Chicago IL 60612. Illinois Institute of Technology Research Institute, Chicago IL 60616. Edison Biotechnology Institute and Department of Biomedical Sciences, Ohio University Athens, OH. Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute Bethesda, MD. Provident Hospital of Cook County, Chicago IL, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 143 EP - 150 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 28 IS - 1 SN - 0143-3334, 0143-3334 KW - Toxicology Abstracts; Oncogenes & Growth Factors Abstracts KW - Growth hormone KW - Data processing KW - Mammary gland KW - Animal models KW - Tumors KW - Clinical trials KW - Models KW - Hyperplasia KW - Carcinogenesis KW - Breast cancer KW - Ovariectomy KW - Estrogen receptors KW - Signal transduction KW - B 26630:Nuclear & DNA-binding proteins KW - X 24300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19840058?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Inhibition+of+estrogen-independent+mammary+carcinogenesis+by+disruption+of+growth+hormone+signaling&rft.au=Zhang%2C+Xiao%3BMehta%2C+Rajendra+G%3BLantvit%2C+Daniel+D%3BCoschigano%2C+Karen+T%3BKopchick%2C+John+J%3BGreen%2C+Jeffrey+E%3BHedayat%2C+Samad%3BChristov%2C+Konstantin+T%3BRay%2C+Vera+H%3BUnterman%2C+Terry+G%3BSwanson%2C+Steven+M&rft.aulast=Zhang&rft.aufirst=Xiao&rft.date=2007-01-01&rft.volume=28&rft.issue=1&rft.spage=143&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Growth hormone; Data processing; Mammary gland; Animal models; Tumors; Clinical trials; Models; Hyperplasia; Carcinogenesis; Breast cancer; Ovariectomy; Estrogen receptors; Signal transduction ER - TY - JOUR T1 - Applications of Rna Interference in Mammalian Systems AN - 19804738; 8190267 AB - Abstract RNA interference (RNAi) can mediate the long- or short-term silencing of gene expression at the DNA, RNA, and/or protein level. Although several triggers of RNAi have been identified, the best characterized of these are small interfering RNAs (siRNAs), which can decrease gene expression through mRNA transcript cleavage, and endogenous microRNAs (miRNAs), which primarily inhibit protein translation. An improved understanding of RNAi has provided new, powerful tools for conducting functional studies in a gene- specific manner. In various applications, RNAi has been used to create model systems, to identify novel molecular targets, to study gene function in a genome-wide fashion, and to create new avenues for clinical therapeutics. Here, we review many of the ongoing applications of RNAi in mammalian and human systems, and discuss how advances in our knowledge of the RNAi machinery have enhanced the use of these technologies. JF - Annual Review of Genomics & Human Genetics AU - Martin, Scott E AU - Caplen, Natasha J AD - Gene Silencing Section, Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, martinsc@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 81 EP - 108 PB - Annual Reviews, Inc., 4139 El Camino Way Box 10139 Palo Alto CA 94303-0139 USA, [mailto:service@annualreviews.org], [URL:http://annualreviews.org] VL - 8 SN - 1527-8204, 1527-8204 KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Gene expression KW - Translation KW - Molecular modelling KW - siRNA KW - Reviews KW - miRNA KW - DNA KW - RNA-mediated interference KW - Transcription KW - G 07880:Human Genetics KW - W 30940:Products KW - N 14830:RNA UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19804738?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annual+Review+of+Genomics+%26+Human+Genetics&rft.atitle=Applications+of+Rna+Interference+in+Mammalian+Systems&rft.au=Martin%2C+Scott+E%3BCaplen%2C+Natasha+J&rft.aulast=Martin&rft.aufirst=Scott&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=81&rft.isbn=&rft.btitle=&rft.title=Annual+Review+of+Genomics+%26+Human+Genetics&rft.issn=15278204&rft_id=info:doi/10.1146%2Fannurev.genom.8.080706.092424 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-06-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Gene expression; Molecular modelling; Translation; siRNA; Reviews; miRNA; DNA; Transcription; RNA-mediated interference DO - http://dx.doi.org/10.1146/annurev.genom.8.080706.092424 ER - TY - JOUR T1 - A New CYP3A5 Variant, CYP3A5*11, Is Shown to Be Defective in Nifedipine Metabolism in a Recombinant cDNA Expression System AN - 19791585; 7207226 AB - A new CYP3A5 variant, CYP3A5*11, was found in a white European subject by DNA sequencing. The CYP3A5*11 allele contains a single nucleotide polymorphism (SNP) (g.3775A>G) in exon 2, which results in a Tyr53Cys substitution, and a g.6986A>G splice change, the latter SNP previously reported in the defective CYP3A5*3 allele. However, the CYP3A5*3 is not a null allele because this variant is associated with leaky splicing, resulting in small amounts of functional protein still being produced. Therefore, we constructed a cDNA coding for the newly identified CYP3A5.11 protein by site-directed mutagenesis, expressed it in Escherichia coli, and partially purified it. Whereas bacteria transformed with wild-type CYP3A5*1 cDNA expressed predominantly cytochrome P450 (P450), those transfected with CYP3A5*11 expressed a significant amount of denatured cytochrome P420 in addition to P450, suggesting the protein to be unstable. CYP3A5.11 exhibited a 38% decrease in the V sub(max) for nifedipine metabolism, a 2.7-fold increase in the K sub(m), and a 4.4-fold decrease in the CL sub(int) of nifedipine compared with CYP3A5.1. A polymerase chain reaction-restriction fragment length polymorphism genotyping procedure was developed and used to genotype DNA of 500 white individuals for CYP3A5*11. No additional examples of this allele were identified. In summary, individuals carrying the rare CYP3A5*11 allele are predicted to have lower metabolism of CYP3A5 substrates than individuals expressing CYP3A5*3. JF - Drug Metabolism and Disposition AU - Lee, Su-Jun AU - van der Heiden, Ilse P AU - Goldstein, Joyce A AU - van Schaik, Ron HN AD - Human Metabolism Section (S.-J.L., J.A.G.), Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 67 EP - 71 PB - Williams & Wilkins, 351 W. Camden St. Baltimore MD 21201 USA, [URL:http://www.lww.com/] VL - 35 IS - 1 SN - 0090-9556, 0090-9556 KW - Microbiology Abstracts B: Bacteriology; Biochemistry Abstracts 2: Nucleic Acids KW - Site-directed mutagenesis KW - Splicing KW - DNA sequencing KW - Single-nucleotide polymorphism KW - Exons KW - Genotyping KW - Escherichia coli KW - Cytochrome P450 KW - Nifedipine KW - J 02320:Cell Biology KW - N 14810:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19791585?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+Metabolism+and+Disposition&rft.atitle=A+New+CYP3A5+Variant%2C+CYP3A5*11%2C+Is+Shown+to+Be+Defective+in+Nifedipine+Metabolism+in+a+Recombinant+cDNA+Expression+System&rft.au=Lee%2C+Su-Jun%3Bvan+der+Heiden%2C+Ilse+P%3BGoldstein%2C+Joyce+A%3Bvan+Schaik%2C+Ron+HN&rft.aulast=Lee&rft.aufirst=Su-Jun&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=67&rft.isbn=&rft.btitle=&rft.title=Drug+Metabolism+and+Disposition&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-06-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Site-directed mutagenesis; DNA sequencing; Splicing; Exons; Single-nucleotide polymorphism; Genotyping; Cytochrome P450; Nifedipine; Escherichia coli ER - TY - JOUR T1 - A novel superfamily containing the beta -grasp fold involved in binding diverse soluble ligands AN - 19787443; 7280270 AB - Domains containing the beta -grasp fold are utilized in a great diversity of physiological functions but their role, if any, in soluble or small molecule ligand recognition is poorly studied. Results Using sensitive sequence and structure similarity searches we identify a novel superfamily containing the beta - grasp fold. They are found in a diverse set of proteins that include the animal vitamin B12 uptake proteins transcobalamin and intrinsic factor, the bacterial polysaccharide export proteins, the competence DNA receptor ComEA, the cob(I)alamin generating enzyme PduS and the Nqo1 subunit of the respiratory electron transport chain. We present evidence that members of this superfamily are likely to bind a range of soluble ligands, including B12. There are two major clades within this superfamily, namely the transcobalamin-like clade and the Nqo1-like clade. The former clade is typified by an insert of a beta -hairpin after the helix of the beta -grasp fold, whereas the latter clade is characterized by an insert between strands 4 and 5 of the core fold. Conclusion Members of both clades within this superfamily are predicted to interact with ligands in a similar spatial location, with their specific inserts playing a role in the process. Both clades are widely represented in bacteria suggesting that this superfamily was derived early in bacterial evolution. The animal lineage appears to have acquired the transcobalamin-like proteins from low GC Gram-positive bacteria, and this might be correlated with the emergence of the ability to utilize B12 produced by gut bacteria.. JF - Biology Direct AU - Burroughs, A Maxwell AU - Balaji, S AU - Iyer, Lakshminarayan M AU - Aravind, L AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA, burrough@ncbi.nlm.nih.gov Y1 - 2007 PY - 2007 DA - 2007 PB - BioMed Central Ltd., Middlesex House 34-42 Cleveland Street London W1T 4LB UK, [mailto:info@biomedcentral.com], [URL:http://www.biomedcentral.com] VL - 2 SN - 1745-6150, 1745-6150 KW - Microbiology Abstracts B: Bacteriology KW - Article No. 4 KW - Guanylate cyclase KW - Digestive tract KW - Vitamin B12 KW - Gram-positive bacteria KW - DNA KW - Enzymes KW - NAD(P)H dehydrogenase (quinone) KW - Polysaccharides KW - Evolution KW - Electron transport chain KW - J 02320:Cell Biology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19787443?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biology+Direct&rft.atitle=A+novel+superfamily+containing+the+beta+-grasp+fold+involved+in+binding+diverse+soluble+ligands&rft.au=Burroughs%2C+A+Maxwell%3BBalaji%2C+S%3BIyer%2C+Lakshminarayan+M%3BAravind%2C+L&rft.aulast=Burroughs&rft.aufirst=A&rft.date=2007-01-01&rft.volume=2&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=Biology+Direct&rft.issn=17456150&rft_id=info:doi/10.1186%2F1745-6150-2-4 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-04-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Guanylate cyclase; Digestive tract; Vitamin B12; Gram-positive bacteria; DNA; Enzymes; NAD(P)H dehydrogenase (quinone); Polysaccharides; Evolution; Electron transport chain DO - http://dx.doi.org/10.1186/1745-6150-2-4 ER - TY - JOUR T1 - A Dominant-Negative Form of the Major Human Abasic Endonuclease Enhances Cellular Sensitivity to Laboratory and Clinical DNA-Damaging Agents AN - 19737317; 7254206 AB - Apurinic/apyrimidinic (AP) endonuclease 1 (APE1) is the primary enzyme in mammals for the repair of abasic sites in DNA, as well as a variety of 3' damages that arise upon oxidation or as products of enzymatic processing. If left unrepaired, APE1 substrates can promote mutagenic and cytotoxic outcomes. We describe herein a dominant-negative form of APE1 that lacks detectable nuclease activity and binds substrate DNA with a 13-fold higher affinity than the wild-type protein. This mutant form of APE1, termed ED, possesses two amino acid substitutions at active site residues Glu super(96) (changed to Gln) and Asp super(210) (changed to Asn). In vitro biochemical assays reveal that ED impedes wild-type APE1 AP site incision function, presumably by binding AP-DNA and blocking normal lesion processing. Moreover, tetracycline-regulated (tet-on) expression of ED in Chinese hamster ovary cells enhances the cytotoxic effects of the laboratory DNA-damaging agents, methyl methanesulfonate (MMS; 5.4-fold) and hydrogen peroxide (1.5-fold). This MMS-induced, ED-dependent cell killing coincides with a hyperaccumulation of AP sites, implying that excessive DNA damage is the cause of cell death. Because an objective of the study was to identify a protein reagent that could be used in targeted gene therapy protocols, the effects of ED on cellular sensitivity to a number of chemotherapeutic compounds was tested. We show herein that ED expression sensitizes Chinese hamster ovary cells to the killing effects of the alkylating agent 1,3-bis(2-chloroethyl)-1-nitrosourea (also known as carmustine) and the chain terminating nucleoside analogue dideoxycytidine (also known as zalcitabine), but not to the radiomimetic bleomycin, the nucleoside analogue {szligbeta}-D-arabinofuranosylcytosine (also known as cytarabine), the topoisomerase inhibitors camptothecin and etoposide, or the cross-linking agents mitomycin C and cisplatin. Transient expression of ED in the human cancer cell line NCI-H1299 enhanced cellular sensitivity to MMS, 1,3-bis(2-chloroethyl)-1-nitrosourea, and dideoxycytidine, demonstrating the potential usefulness of this strategy in the treatment of human tumors. (Mol Cancer Res 2007; 5(1):61-70) JF - Molecular Cancer Research AU - McNeill, Daniel R AU - Wilson, David MIII AD - Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, Maryland Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 61 EP - 70 PB - American Association for Cancer Research, 615 Chestnut St., 17th Floor Philadelphia PA 19106-4404 USA, [URL:http://www.aacr.org/] VL - 5 IS - 1 SN - 1541-7786, 1541-7786 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Amino acid substitution KW - Nuclease KW - Mitomycin C KW - Tumor cell lines KW - Cisplatin KW - Hydrogen peroxide KW - Endonuclease KW - Etoposide KW - dideoxycytidine KW - Methyl methanesulfonate KW - Alkylating agents KW - cytarabine KW - Gene therapy KW - Zalcitabine KW - Enzymes KW - Tumors KW - Bleomycin KW - Camptothecin KW - Cancer KW - nucleoside analogs KW - DNA damage KW - Cell death KW - Cytotoxicity KW - Oxidation KW - AP endonuclease KW - W 30905:Medical Applications KW - N 14820:DNA Metabolism & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19737317?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Cancer+Research&rft.atitle=A+Dominant-Negative+Form+of+the+Major+Human+Abasic+Endonuclease+Enhances+Cellular+Sensitivity+to+Laboratory+and+Clinical+DNA-Damaging+Agents&rft.au=McNeill%2C+Daniel+R%3BWilson%2C+David+MIII&rft.aulast=McNeill&rft.aufirst=Daniel&rft.date=2007-01-01&rft.volume=5&rft.issue=1&rft.spage=61&rft.isbn=&rft.btitle=&rft.title=Molecular+Cancer+Research&rft.issn=15417786&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-08-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Amino acid substitution; Nuclease; Mitomycin C; Tumor cell lines; Cisplatin; Hydrogen peroxide; Endonuclease; Etoposide; dideoxycytidine; Alkylating agents; Methyl methanesulfonate; cytarabine; Gene therapy; Zalcitabine; Enzymes; Tumors; Bleomycin; Cancer; Camptothecin; nucleoside analogs; DNA damage; Cytotoxicity; Cell death; Oxidation; AP endonuclease ER - TY - JOUR T1 - Body mass index, physical activity, and dietary behaviors among members of an urban community fitness center: a questionnaire survey AN - 19723280; 9034154 AB - Background Development of effective behavioral interventions to promote weight control and physical activity among diverse, underserved populations is a public health priority. Community focused wellness organizations, such as YMCAs, could provide a unique channel with which to reach such populations. This study assessed health behaviors and related characteristics of members of an urban YMCA facility. Methods We surveyed 135 randomly selected members of an urban YMCA facility in Massachusetts to examine self-reported (1) physical activity, (2) dietary behaviors, (3) body mass index, and (4) correlates of behavior change among short-term (i.e., one year or less) and long-term (i.e., more than one year) members. Chi-square tests were used to assess bivariate associations between variables, and multivariate linear regression models were fit to examine correlates of health behaviors and weight status. Results Eighty-nine percent of short-term and 94% of long-term members reported meeting current physical activity recommendations. Only 24% of short-term and 19% of long-term members met fruit and vegetable consumption recommendations, however, and more than half were overweight or obese. Length of membership was not significantly related to weight status, dietary behaviors, or physical activity. Most respondents were interested in changing health behaviors, in the preparation stage of change, and had high levels of self-efficacy to change behaviors. Short-term members had less education (p = 0.02), lower household incomes (p = 0.02), and were less likely to identify as white (p = 0.005) than long-term members. In multivariate models, females had lower BMI than males (p = 0.003) and reported less physical activity (p = 0.008). Physical activity was also inversely associated with age (p = 0.0004) and education (p = 0.02). Conclusion Rates of overweight/obesity and fruit and vegetable consumption suggested that there is a need for a weight control intervention among members of an urban community YMCA. Membership in such a community wellness facility alone might not be sufficient to help members maintain a healthy weight. The data indicate that YMCA members are interested in making changes in their dietary and physical activity behaviors. Targeting newer YMCA members might be an effective way of reaching underserved populations. These data will help inform the development of a weight control intervention tailored to this setting. JF - BMC Public Health AU - Kaphingst, Kimberly A AU - Bennett, Gary G AU - Sorensen, Glorian AU - Kaphingst, Karen M AU - O'Neil, Amy E AU - McInnis, Kyle AD - Center for Community-Based Research, Dana-Farber Cancer Institute, Boston, MA 02115, USA, kkaphing@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 181 PB - BioMed Central Ltd., Middlesex House VL - 7 SN - 1471-2458, 1471-2458 KW - Physical Education Index UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19723280?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Public+Health&rft.atitle=Body+mass+index%2C+physical+activity%2C+and+dietary+behaviors+among+members+of+an+urban+community+fitness+center%3A+a+questionnaire+survey&rft.au=Kaphingst%2C+Kimberly+A%3BBennett%2C+Gary+G%3BSorensen%2C+Glorian%3BKaphingst%2C+Karen+M%3BO%27Neil%2C+Amy+E%3BMcInnis%2C+Kyle&rft.aulast=Kaphingst&rft.aufirst=Kimberly&rft.date=2007-01-01&rft.volume=7&rft.issue=&rft.spage=181&rft.isbn=&rft.btitle=&rft.title=BMC+Public+Health&rft.issn=14712458&rft_id=info:doi/10.1186%2F1471-2458-7-181 LA - English DB - Physical Education Index N1 - Date revised - 2009-04-01 N1 - Last updated - 2011-12-14 DO - http://dx.doi.org/10.1186/1471-2458-7-181 ER - TY - JOUR T1 - The two faces of short-range evolutionary dynamics of regulatory modes in bacterial transcriptional regulatory networks AN - 19700283; 7456434 AB - Studies on the conservation of the inferred transcriptional regulatory network of prokaryotes have suggested that specific transcription factors are less-widely conserved in comparison to their target genes. This observation implied that, at large evolutionary distances, the turnover of specific transcription factors through loss and non-orthologous displacement might be a major factor in the adaptive radiation of prokaryotes. However, the recent work of Hershberg and Margalit suggests that, at shorter phylogenetic scales, the evolutionary dynamics of the bacterial transcriptional regulatory network might exhibit distinct patterns. The authors find previously unnoticed relationships between the regulatory mode (activation or repression), the number of regulatory interactions and their conservation patterns in [gamma]- proteobacteria. These relationships might be shaped by the differences in the adaptive value and mode of operation of different regulatory interactions. BioEssays 29:625-629, 2007. JF - Bioessays AU - Balaji, S AU - Aravind, L AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, sbalaji@ncbi.nlm.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 625 EP - 629 PB - John Wiley & Sons, Inc., 111 River Street Hoboken NJ 07030 USA, [mailto:custserv@wiley.com], [URL:http://www.wiley.com/] VL - 29 IS - 7 SN - 0265-9247, 0265-9247 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Phylogeny KW - Transcription factors KW - Conservation KW - Prokaryotes KW - Proteobacteria KW - Adaptive radiation KW - Evolution KW - J 02310:Genetics & Taxonomy KW - A 01490:Miscellaneous KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19700283?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Bioessays&rft.atitle=The+two+faces+of+short-range+evolutionary+dynamics+of+regulatory+modes+in+bacterial+transcriptional+regulatory+networks&rft.au=Balaji%2C+S%3BAravind%2C+L&rft.aulast=Balaji&rft.aufirst=S&rft.date=2007-01-01&rft.volume=29&rft.issue=7&rft.spage=625&rft.isbn=&rft.btitle=&rft.title=Bioessays&rft.issn=02659247&rft_id=info:doi/10.1002%2Fbies.20600 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Phylogeny; Transcription factors; Conservation; Prokaryotes; Evolution; Adaptive radiation; Proteobacteria DO - http://dx.doi.org/10.1002/bies.20600 ER - TY - JOUR T1 - Evidence-based medicine among internal medicine residents in a community AN - 19690961; 7458208 AB - Background: This study implemented and evaluated a point-of-care, wireless JF - BMC Medical Informatics and Decision Making AU - Leon, Sergio A AU - Fontelo, Paul AU - Green, Linda AU - Ackerman, Michael AU - Liu, Fang AD - Office of High Performance Computing and Communications, 8600 Rockville, sleon@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 PB - BioMed Central Ltd., Middlesex House 34-42 Cleveland Street London W1T 4LB UK, [mailto:info@biomedcentral.com], [URL:http://www.biomedcentral.com] VL - 7 SN - 1472-6947, 1472-6947 KW - Biotechnology and Bioengineering Abstracts KW - Article No. 5 UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19690961?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Medical+Informatics+and+Decision+Making&rft.atitle=Evidence-based+medicine+among+internal+medicine+residents+in+a+community&rft.au=Leon%2C+Sergio+A%3BFontelo%2C+Paul%3BGreen%2C+Linda%3BAckerman%2C+Michael%3BLiu%2C+Fang&rft.aulast=Leon&rft.aufirst=Sergio&rft.date=2007-01-01&rft.volume=7&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=BMC+Medical+Informatics+and+Decision+Making&rft.issn=14726947&rft_id=info:doi/10.1186%2F1472-6947-7-5 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 DO - http://dx.doi.org/10.1186/1472-6947-7-5 ER - TY - JOUR T1 - Transmission-blocking activity induced by malaria vaccine candidates Pfs25/Pvs25 is a direct and predictable function of antibody titer AN - 19675076; 9034713 AB - Background Mosquito stage malaria vaccines are designed to induce an immune response in the human host that will block the parasite's growth in the mosquito and consequently block transmission of the parasite. A mosquito membrane-feeding assay (MFA) is used to test transmission-blocking activity (TBA), but in this technique cannot accommodate many samples. A clear understanding of the relationship between antibody levels and TBA may allow ELISA determinations to be used to predict TBA and assist in planning vaccine development. Methods Rabbit anti-Pfs25 sera and monkey anti-Pvs25 sera were generated and the antibody titers were determined by a standardized ELISA. The biological activity of the same sera was tested by MFA using Plasmodium gametocytes (cultured Plasmodium falciparum or Plasmodium vivax from malaria patients) and Anopheles mosquitoes. Results Anti-Pfs25 and anti-Pvs25 sera showed that ELISA antibody units correlate with the percent reduction in the oocyst density per mosquito (Spearman Rank correlations: 0.934 and 0.616, respectively), and fit a hyperbolic curve when percent reduction in oocyst density is plotted against antibody units of the tested sample. Antibody levels also correlated with the number of mosquitoes that failed to become infected, and this proportion can be calculated from the reduction in oocyst numbers and the distribution of oocysts per infected mosquito in control group. Conclusion ELISA data may be used as a surrogate for the MFA to evaluate transmission-blocking vaccine efficacy. This will facilitate the evaluation of transmission-blocking vaccines and implementation of this malaria control strategy. JF - Malaria Journal AU - Miura, Kazutoyo AU - Keister, David B AU - Muratova, Olga V AU - Sattabongkot, Jetsumon AU - Long, Carole A AU - Saul, Allan AD - Malaria Vaccine Development Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA, kmiura@niaid.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 107 PB - BioMed Central Ltd., Middlesex House VL - 6 SN - 1475-2875, 1475-2875 KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Q5 01522:Protective measures and control UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19675076?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Malaria+Journal&rft.atitle=Transmission-blocking+activity+induced+by+malaria+vaccine+candidates+Pfs25%2FPvs25+is+a+direct+and+predictable+function+of+antibody+titer&rft.au=Miura%2C+Kazutoyo%3BKeister%2C+David+B%3BMuratova%2C+Olga+V%3BSattabongkot%2C+Jetsumon%3BLong%2C+Carole+A%3BSaul%2C+Allan&rft.aulast=Miura&rft.aufirst=Kazutoyo&rft.date=2007-01-01&rft.volume=6&rft.issue=&rft.spage=107&rft.isbn=&rft.btitle=&rft.title=Malaria+Journal&rft.issn=14752875&rft_id=info:doi/10.1186%2F1475-2875-6-107 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2011-12-14 DO - http://dx.doi.org/10.1186/1475-2875-6-107 ER - TY - JOUR T1 - Susceptibility of Anopheles gambiae and Anopheles stephensi to tropical isolates of Plasmodium falciparum AN - 19673470; 9034745 AB - Background The susceptibility of anopheline mosquito species to Plasmodium infection is known to be variable with some mosquitoes more permissive to infection than others. Little work, however, has been carried out investigating the susceptibility of major malaria vectors to geographically diverse tropical isolates of Plasmodium falciparum aside from examining the possibility of infection extending its range from tropical regions into more temperate zones. Methods This study investigates the susceptibility of two major tropical mosquito hosts (Anopheles gambiae and Anopheles stephensi) to P. falciparum isolates of different tropical geographical origins. Cultured parasite isolates were fed via membrane feeders simultaneously to both mosquito species and the resulting mosquito infections were compared. Results Infection prevalence was variable with African parasites equally successful in both mosquito species, Thai parasites significantly more successful in An. stephensi, and PNG parasites largely unsuccessful in both species. Conclusion Infection success of P. falciparum was variable according to geographical origin of both the parasite and the mosquito. Data presented raise the possibility that local adaptation of tropical parasites and mosquitoes has a role to play in limiting gene flow between allopatric parasite populations. JF - Malaria Journal AU - Hume, Jennifer CC AU - Tunnicliff, Mark AU - Ranford-Cartwright, Lisa C AU - Day, Karen P AD - Peter Medawar Building for Pathogen Research and Department of Zoology, South Park road, University of Oxford, Oxford OX1 3SY, UK, humej@niaid.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 139 PB - BioMed Central Ltd., Middlesex House VL - 6 SN - 1475-2875, 1475-2875 KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Q5 01524:Public health, medicines, dangerous organisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19673470?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Malaria+Journal&rft.atitle=Susceptibility+of+Anopheles+gambiae+and+Anopheles+stephensi+to+tropical+isolates+of+Plasmodium+falciparum&rft.au=Hume%2C+Jennifer+CC%3BTunnicliff%2C+Mark%3BRanford-Cartwright%2C+Lisa+C%3BDay%2C+Karen+P&rft.aulast=Hume&rft.aufirst=Jennifer&rft.date=2007-01-01&rft.volume=6&rft.issue=&rft.spage=139&rft.isbn=&rft.btitle=&rft.title=Malaria+Journal&rft.issn=14752875&rft_id=info:doi/10.1186%2F1475-2875-6-139 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2011-12-14 DO - http://dx.doi.org/10.1186/1475-2875-6-139 ER - TY - JOUR T1 - Standardizing estimates of the Plasmodium falciparum parasite rate AN - 19670708; 9034737 AB - Background The Plasmodium falciparum parasite rate (PfPR) is a commonly reported index of malaria transmission intensity. PfPR rises after birth to a plateau before declining in older children and adults. Studies of populations with different age ranges generally report average PfPR, so age is an important source of heterogeneity in reported PfPR data. This confounds simple comparisons of PfPR surveys conducted at different times or places. Methods Several algorithms for standardizing PfPR were developed using 21 studies that stratify in detail PfPR by age. An additional 121 studies were found that recorded PfPR from the same population over at least two different age ranges; these paired estimates were used to evaluate these algorithms. The best algorithm was judged to be the one that described most of the variance when converting the PfPR pairs from one age-range to another. Results The analysis suggests that the relationship between PfPR and age is predictable across the observed range of malaria endemicity. PfPR reaches a peak after about two years and remains fairly constant in older children until age ten before declining throughout adolescence and adulthood. The PfPR pairs were poorly correlated; using one to predict the other would explain only 5% of the total variance. By contrast, the PfPR predicted by the best algorithm explained 72% of the variance. Conclusion The PfPR in older children is useful for standardization because it has good biological, epidemiological and statistical properties. It is also historically consistent with the classical categories of hypoendemic, mesoendemic and hyperendemic malaria. This algorithm provides a reliable method for standardizing PfPR for the purposes of comparing studies and mapping malaria endemicity. The scripts for doing so are freely available to all. JF - Malaria Journal AU - Smith, David L AU - Guerra, Carlos A AU - Snow, Robert W AU - Hay, Simon I AD - Fogarty International Center, National Institutes of Health, Building 16, 16 Center Drive, Bethesda, Maryland 20892, USA, davesmith@ufl.edu Y1 - 2007 PY - 2007 DA - 2007 SP - 131 PB - BioMed Central Ltd., Middlesex House VL - 6 SN - 1475-2875, 1475-2875 KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Microbiology Abstracts C: Algology, Mycology & Protozoology KW - Q5 01524:Public health, medicines, dangerous organisms UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19670708?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Malaria+Journal&rft.atitle=Standardizing+estimates+of+the+Plasmodium+falciparum+parasite+rate&rft.au=Smith%2C+David+L%3BGuerra%2C+Carlos+A%3BSnow%2C+Robert+W%3BHay%2C+Simon+I&rft.aulast=Smith&rft.aufirst=David&rft.date=2007-01-01&rft.volume=6&rft.issue=&rft.spage=131&rft.isbn=&rft.btitle=&rft.title=Malaria+Journal&rft.issn=14752875&rft_id=info:doi/10.1186%2F1475-2875-6-131 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-03-01 N1 - Last updated - 2011-12-14 DO - http://dx.doi.org/10.1186/1475-2875-6-131 ER - TY - JOUR T1 - Employer and Healthcare Policy Interventions Aimed at Adult Obesity AN - 19656911; 8791199 AB - Abstract not available. JF - American Journal of Preventive Medicine AU - Fuemmeler, Bernard F AU - Baffi, Charlie AU - Masse, Louise C AU - Atienza, Audie A AU - Evans, W Doug AD - National Cancer Institute, Bethesda, Maryland, bernard.fuemmeler@duke.edu Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 44 EP - 51 PB - Elsevier Science, The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 32 IS - 1 SN - 0749-3797, 0749-3797 KW - Physical Education Index KW - Obesity KW - Employers KW - Policy KW - Adults KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19656911?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Preventive+Medicine&rft.atitle=Employer+and+Healthcare+Policy+Interventions+Aimed+at+Adult+Obesity&rft.au=Fuemmeler%2C+Bernard+F%3BBaffi%2C+Charlie%3BMasse%2C+Louise+C%3BAtienza%2C+Audie+A%3BEvans%2C+W+Doug&rft.aulast=Fuemmeler&rft.aufirst=Bernard&rft.date=2007-01-01&rft.volume=32&rft.issue=1&rft.spage=44&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Preventive+Medicine&rft.issn=07493797&rft_id=info:doi/10.1016%2Fj.amepre.2006.09.003 LA - English DB - Physical Education Index N1 - Date revised - 2009-02-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Adults; Obesity; Employers; Policy DO - http://dx.doi.org/10.1016/j.amepre.2006.09.003 ER - TY - JOUR T1 - Comparing Genetically Engineered Mouse Mammary Cancer Models with Human Breast Cancer by Expression Profiling AN - 19618093; 8717186 AB - Breast cancer is a heterogeneous disease, and much of the molecular basis for this heterogeneity is being unraveled using advanced genomic technologies. More recently, global transcriptional profiling has proven to be an effective new tool for characterizing human tumors. Genomic ``signatures'' have been developed that classify tumors with varying prognoses and responses to treatment. Recent studies have begun to extend the use of global transcriptional profiling to better characterize genetically engineered mouse (GEM) models of breast cancer, which will improve the ability to translate basic research advances into clinical advances. GEM models of mammary carcinoma have proven to be invaluable tools to gain insight into mechanisms underlying tumor initiation, progression, and therapeutic responses in an in vivo system where tumors spontaneously develop in an appropriate tissue environment. This review will discuss the use of transcriptional profiling of breast cancer in tumors from both human patients and GEM models to improve prognostic measures, examine mechanisms of tumor initiation and progression, identify novel therapeutic targets, and improve pre-clinical testing for drug development. Together, these advances offer a framework for classifying human tumors, identifying appropriate GEM models for specific experimental purposes, and utilizing the combined data to identify more specific and effective cancer therapies. JF - Breast Disease AU - Shoushtari, Alexander N AU - Michalowska, Aleksandra M AU - Green, Jeffrey E AD - Laboratory of Cancer Biology and Genetics, National Cancer Institute, Bethesda, MD 20892, USA Y1 - 2007 PY - 2007 DA - 2007 SP - 39 EP - 51 PB - IOS Press, Nieuwe Hemweg 6B VL - 28 SN - 0888-6008, 0888-6008 KW - Genetics Abstracts; Biotechnology and Bioengineering Abstracts KW - Data processing KW - Mammary gland KW - Genetic engineering KW - Animal models KW - Breast cancer KW - Transcription KW - Drug development KW - Tumors KW - genomics KW - Models KW - Carcinoma KW - W 30925:Genetic Engineering KW - G 07730:Development & Cell Cycle UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19618093?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Breast+Disease&rft.atitle=Comparing+Genetically+Engineered+Mouse+Mammary+Cancer+Models+with+Human+Breast+Cancer+by+Expression+Profiling&rft.au=Shoushtari%2C+Alexander+N%3BMichalowska%2C+Aleksandra+M%3BGreen%2C+Jeffrey+E&rft.aulast=Shoushtari&rft.aufirst=Alexander&rft.date=2007-01-01&rft.volume=28&rft.issue=&rft.spage=39&rft.isbn=&rft.btitle=&rft.title=Breast+Disease&rft.issn=08886008&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Data processing; Mammary gland; Genetic engineering; Animal models; Transcription; Breast cancer; Drug development; genomics; Tumors; Carcinoma; Models ER - TY - JOUR T1 - SpliceMiner: a high-throughput database implementation of the NCBI Evidence Viewer for microarray splice variant analysis AN - 19612553; 7332965 AB - There are many fewer genes in the human genome than there are expressed transcripts. Alternative splicing is the reason. Alternatively spliced transcripts are often specific to tissue type, developmental stage, environmental condition, or disease state. Accurate analysis of microarray expression data and design of new arrays for alternative splicing require assessment of probes at the sequence and exon levels. Description SpliceMiner is a web interface for querying Evidence Viewer Database (EVDB). EVDB is a comprehensive, non-redundant compendium of splice variant data for human genes. We constructed EVDB as a queryable implementation of the NCBI Evidence Viewer (EV). EVDB is based on data obtained from NCBI Entrez Gene and EV. The automated EVDB build process uses only complete coding sequences, which may or may not include partial or complete 5' and 3' UTRs, and filters redundant splice variants. Unlike EV, which supports only one-at-a-time queries, SpliceMiner supports high-throughput batch queries and provides results in an easily parsable format. SpliceMiner maps probes to splice variants, effectively delineating the variants identified by a probe. Conclusion EVDB can be queried by gene symbol, genomic coordinates, or probe sequence via a user-friendly web-based tool we call SpliceMiner ( JF - BMC Bioinformatics AU - Kahn, Ari B AU - Ryan, Michael C AU - Liu, Hongfang AU - Zeeberg, Barry R AU - Jamison, D Curtis AU - Weinstein, John N AD - Department of Bioinformatics, George Mason University, Fairfax, Virginia, USA, arik@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 PB - BioMed Central Ltd., Middlesex House 34-42 Cleveland Street London W1T 4LB UK, [mailto:info@biomedcentral.com], [URL:http://www.biomedcentral.com] VL - 8 SN - 1471-2105, 1471-2105 KW - Biotechnology and Bioengineering Abstracts KW - Article No. 75 KW - Genomes KW - Exons KW - DNA probes KW - Developmental stages KW - DNA microarrays KW - Alternative splicing KW - Filters KW - Databases KW - Bioinformatics KW - genomics KW - Environmental conditions KW - Gene mapping KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19612553?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Bioinformatics&rft.atitle=SpliceMiner%3A+a+high-throughput+database+implementation+of+the+NCBI+Evidence+Viewer+for+microarray+splice+variant+analysis&rft.au=Kahn%2C+Ari+B%3BRyan%2C+Michael+C%3BLiu%2C+Hongfang%3BZeeberg%2C+Barry+R%3BJamison%2C+D+Curtis%3BWeinstein%2C+John+N&rft.aulast=Kahn&rft.aufirst=Ari&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=BMC+Bioinformatics&rft.issn=14712105&rft_id=info:doi/10.1186%2F1471-2105-8-75 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Filters; Genomes; Databases; Exons; DNA probes; Developmental stages; genomics; Bioinformatics; Environmental conditions; DNA microarrays; Gene mapping; Alternative splicing DO - http://dx.doi.org/10.1186/1471-2105-8-75 ER - TY - JOUR T1 - Immunotoxin Treatment of Cancer AN - 19594486; 7320739 AB - Immunotoxins are proteins used to treat cancer that are composed of an antibody fragment linked to a toxin. The immunotoxin binds to a surface antigen on a cancer cell, enters the cell by endocytosis, and kills it. The most potent immunotoxins are made from bacterial and plant toxins. Refinements over many years have produced recombinant immunotoxins; these therapeutic proteins are made using protein engineering. Individual immunotoxins are designed to treat specific cancers. To date, most success has been achieved treating hematologic tumors. Obstacles to successful treatment of solid tumors include poor penetration into tumor masses and the immune response to the toxin component of the immunotoxin, which limits the number of cycles that can be given. Strategies to overcome these limitations are being pursued. JF - Annual Review of Medicine AU - Pastan, Ira AU - Hassan, Raffit AU - FitzGerald, David J AU - Kreitman, Robert J AD - Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, pastani@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 221 EP - 237 PB - Annual Reviews, Inc., 4139 El Camino Way Box 10139 Palo Alto CA 94303-0139 USA, [mailto:service@annualreviews.org], [URL:http://annualreviews.org] VL - 58 SN - 0066-4219, 0066-4219 KW - Microbiology Abstracts B: Bacteriology; Biotechnology and Bioengineering Abstracts; Immunology Abstracts KW - Endocytosis KW - Antibodies KW - Protein engineering KW - surface antigens KW - Solid tumors KW - Reviews KW - Tumors KW - Immune response KW - Immunotoxins KW - Cancer KW - Toxins KW - F 06915:Cancer Immunology KW - J 02350:Immunology KW - W 30915:Pharmaceuticals & Vaccines UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19594486?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Annual+Review+of+Medicine&rft.atitle=Immunotoxin+Treatment+of+Cancer&rft.au=Pastan%2C+Ira%3BHassan%2C+Raffit%3BFitzGerald%2C+David+J%3BKreitman%2C+Robert+J&rft.aulast=Pastan&rft.aufirst=Ira&rft.date=2007-01-01&rft.volume=58&rft.issue=&rft.spage=221&rft.isbn=&rft.btitle=&rft.title=Annual+Review+of+Medicine&rft.issn=00664219&rft_id=info:doi/10.1146%2Fannurev.med.58.070605.115320 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Endocytosis; Antibodies; Protein engineering; Solid tumors; surface antigens; Reviews; Immune response; Tumors; Toxins; Cancer; Immunotoxins DO - http://dx.doi.org/10.1146/annurev.med.58.070605.115320 ER - TY - JOUR T1 - Parent involvement in novice teen driving: Rationale, evidence of effects, and potential for enhancing graduated driver licensing effectiveness AN - 19580364; 8549553 AB - Motor-vehicle crash rates are highly elevated immediately after licensure and then decline gradually over a period of years. Young age, risk taking, and inexperience contribute to the problem, but inexperience is particularly important early on. Driving is like other complex, skilled behaviors in which subtle improvements in perception and judgment develop gradually over a period of years. After all, safe driving is more a matter of attention and perception than physical management of the vehicle. Inexperience is particularly linked to driving performance and safety outcomes under certain driving conditions, with driving at night and with teen passengers as the most important cases. Surprisingly, driving outcomes do not appear to be affected by the pre-license training or supervised practice driving. Given the limits of training, safety effects can best be achieved by countermeasures that delay licensure or limit novice teen driving under high risk driving conditions while novices gain experience and develop safety competence. The two complementary approaches of Graduated Driver Licensing policies and parent management have been shown to provide safety effects by limiting the driving conditions of novice teenagers. JF - Journal of Safety Research AU - Simons-Morton, Bruce AD - Prevention Research Branch, Division of Epidemiology, Statistics, and Prevention Research; National Institute of Child Health and Human Development, 6100 Executive Blvd, 7B05, Bethesda, MD, 20892-7510, USA, mortonb@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 193 EP - 202 PB - Elsevier Science, P.O. Box 800 Kidlington Oxford OX5 1DX UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 38 IS - 2 SN - 0022-4375, 0022-4375 KW - Risk Abstracts; Health & Safety Science Abstracts KW - Age KW - Accidents KW - Driving ability KW - risk taking KW - graduated licensing KW - Adolescents KW - Training KW - Perception KW - Traffic safety KW - H 2000:Transportation KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19580364?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Safety+Research&rft.atitle=Parent+involvement+in+novice+teen+driving%3A+Rationale%2C+evidence+of+effects%2C+and+potential+for+enhancing+graduated+driver+licensing+effectiveness&rft.au=Simons-Morton%2C+Bruce&rft.aulast=Simons-Morton&rft.aufirst=Bruce&rft.date=2007-01-01&rft.volume=38&rft.issue=2&rft.spage=193&rft.isbn=&rft.btitle=&rft.title=Journal+of+Safety+Research&rft.issn=00224375&rft_id=info:doi/10.1016%2Fj.jsr.2007.02.007 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-11-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Training; graduated licensing; Perception; Accidents; Adolescents; risk taking; Traffic safety; Driving ability; Age DO - http://dx.doi.org/10.1016/j.jsr.2007.02.007 ER - TY - JOUR T1 - 3,4-Methylenedioxymethamphetamine (MDMA) neurotoxicity in rats: a reappraisal of past and present findings AN - 19579819; 7300498 AB - Rationale: 3,4-Methylenedioxymethamphetamine (MDMA) is a widely abused illicit drug. In animals, high-dose administration of MDMA produces deficits in serotonin (5-HT) neurons (e.g., depletion of forebrain 5-HT) that have been interpreted as neurotoxicity. Whether such 5-HT deficits reflect neuronal damage is a matter of ongoing debate. Objective: The present paper reviews four specific issues related to the hypothesis of MDMA neurotoxicity in rats: (1) the effects of MDMA on monoamine neurons, (2) the use of 'interspecies scaling' to adjust MDMA doses across species, (3) the effects of MDMA on established markers of neuronal damage, and (4) functional impairments associated with MDMA-induced 5-HT depletions. Results: MDMA is a substrate for monoamine transporters, and stimulated release of 5-HT, NE, and DA mediates effects of the drug. MDMA produces neurochemical, endocrine, and behavioral actions in rats and humans at equivalent doses (e.g., 1-2 mg/kg), suggesting that there is no reason to adjust doses between these species. Typical doses of MDMA causing long-term 5-HT depletions in rats (e.g., 10-20 mg/kg) do not reliably increase markers of neurotoxic damage such as cell death, silver staining, or reactive gliosis. MDMA-induced 5-HT depletions are accompanied by a number of functional consequences including reductions in evoked 5-HT release and changes in hormone secretion. Perhaps more importantly, administration of MDMA to rats induces persistent anxiety-like behaviors in the absence of measurable 5-HT deficits. Conclusions: MDMA-induced 5-HT depletions are not necessarily synonymous with neurotoxic damage. However, doses of MDMA which do not cause long-term 5-HT depletions can have protracted effects on behavior, suggesting even moderate doses of the drug may pose risks. JF - Psychopharmacology AU - Baumann, Michael H AU - Wang, Xiaoying AU - Rothman, Richard B AD - Clinical Psychopharmacology Section, Intramural Research Program (IRP), National Institute on Drug Abuse (NIDA), National Institutes of Health (NIH), 5500 Nathan Shock Drive, Baltimore, MD, 21224, USA, mbaumann@intra.nida.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 407 EP - 424 PB - Springer-Verlag (Berlin), Heidelberger Platz 3 Berlin 14197 Germany, [mailto:subscriptions@springer.de], [URL:http://www.springer.de/] VL - 189 IS - 4 SN - 0033-3158, 0033-3158 KW - Toxicology Abstracts; CSA Neurosciences Abstracts KW - monoamines KW - Anxiety KW - Monoamine transporter KW - Drug abuse KW - Hormones KW - MDMA KW - Serotonin KW - Forebrain KW - Cell death KW - Reviews KW - Neurons KW - Neurotoxicity KW - gliosis KW - Scaling KW - X 24380:Social Poisons & Drug Abuse KW - N3 11001:Behavioral and Cognitive Neuroscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19579819?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Psychopharmacology&rft.atitle=3%2C4-Methylenedioxymethamphetamine+%28MDMA%29+neurotoxicity+in+rats%3A+a+reappraisal+of+past+and+present+findings&rft.au=Baumann%2C+Michael+H%3BWang%2C+Xiaoying%3BRothman%2C+Richard+B&rft.aulast=Baumann&rft.aufirst=Michael&rft.date=2007-01-01&rft.volume=189&rft.issue=4&rft.spage=407&rft.isbn=&rft.btitle=&rft.title=Psychopharmacology&rft.issn=00333158&rft_id=info:doi/10.1007%2Fs00213-006-0322-6 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-04-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - monoamines; Anxiety; Monoamine transporter; Drug abuse; MDMA; Hormones; Serotonin; Forebrain; Cell death; Neurons; Reviews; Neurotoxicity; gliosis; Scaling DO - http://dx.doi.org/10.1007/s00213-006-0322-6 ER - TY - JOUR T1 - Water-soluble ions in nano/ultrafine/fine/coarse particles collected near a busy road and at a rural site AN - 19575270; 7492026 AB - This study investigated water-soluble ions in the sized particles (particularly nano (PM0.01-0.056)/ultrafine (PM0.01-0.1)) collected using MOUDI and Nano-MOUDI samplers near a busy road site and at a rural site. The analytical results demonstrate that nano and coarse particles exhibited the highest (16.3%) and lowest (8.37%) nitrate mass ratios, respectively. The mass ratio of NO3- was higher than that of SO42- in all the sized particles at the traffic site. The secondary aerosols all displayed trimodal distributions. The aerosols in ultrafine particles collected at the roadside site exhibited Aitken mode distributions indicating they were of local origin. This finding was not observed for those ultrafine particles collected at the rural site. The mass median diameters (MMDs) of the nano, ultrafine, and fine particles were smaller at the traffic site than at the rural site, possibly related to the contribution of mobile engine emissions. JF - Environmental Pollution AU - Lin, Chih-Chung AU - Chen, Shui-Jen AU - Huang, Kuo-Lin AU - Lee, Wen-Jhy AU - Lin, Wen-Yinn AU - Liao, Chiu-Jung AU - Chaung, Hso-Chi AU - Chiu, Chuen-Huey AD - Department of Environmental Engineering and Science, National Pingtung University of Science and Technology, 1 Hseuh Fu RD., Nei Pu, PingTung 91201, Taiwan, chensj@mail.npust.edu.tw Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 562 EP - 570 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 145 IS - 2 SN - 0269-7491, 0269-7491 KW - Toxicology Abstracts; Pollution Abstracts KW - Traffic-associated particles KW - Nanoparticles KW - Ultrafine KW - Water-soluble ions KW - Size distributions KW - Ions KW - Nitrate KW - Aerosols KW - Nitrates KW - roadsides KW - Particulates KW - Samplers KW - Traffic KW - traffic KW - Roadsides KW - Emissions KW - Cadmium KW - Pollution KW - Rural areas KW - P 0000:AIR POLLUTION KW - X 24490:Other UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19575270?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Environmental+Pollution&rft.atitle=Water-soluble+ions+in+nano%2Fultrafine%2Ffine%2Fcoarse+particles+collected+near+a+busy+road+and+at+a+rural+site&rft.au=Lin%2C+Chih-Chung%3BChen%2C+Shui-Jen%3BHuang%2C+Kuo-Lin%3BLee%2C+Wen-Jhy%3BLin%2C+Wen-Yinn%3BLiao%2C+Chiu-Jung%3BChaung%2C+Hso-Chi%3BChiu%2C+Chuen-Huey&rft.aulast=Lin&rft.aufirst=Chih-Chung&rft.date=2007-01-01&rft.volume=145&rft.issue=2&rft.spage=562&rft.isbn=&rft.btitle=&rft.title=Environmental+Pollution&rft.issn=02697491&rft_id=info:doi/10.1016%2Fj.envpol.2006.04.023 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Nitrate; Ions; Aerosols; Roadsides; Samplers; Pollution; Traffic; traffic; Nitrates; roadsides; Emissions; Cadmium; Particulates; Rural areas DO - http://dx.doi.org/10.1016/j.envpol.2006.04.023 ER - TY - JOUR T1 - Tryptophanase in sRNA control of the Escherichia coli cell cycle AN - 19563469; 7228977 AB - The field of gene regulation underwent a major revolution with the discovery of small non-coding RNAs (sRNAs) and the various roles they play in organisms from bacteria to man. Escherichia coli has more than 60 sRNAs that are transcribed primarily from intergenic regions. They usually target the leader region of mRNAs and prevent their translation. Protein targets are relatively rare. In this issue of Molecular Microbiology, Chant and Summers provide an example of a totally unexpected protein target. They show that dimers of plasmid ColE1 make an sRNA that interacts directly with the enzyme tryptophanase and enhances its affinity for its substrate, tryptophan. A breakdown product, indole, then arrests cell division until the dimers are resolved to monomers. The monomerization helps to prevent plasmid loss. Targeting a catabolic enzyme to buy time for recombination is an amazing example of adaptation, which illustrates the power of a selfish element (a plasmid in this case) to exploit the host cell machinery to its advantage. JF - Molecular Microbiology AU - Chattoraj, Dhruba K Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1 EP - 3 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 63 IS - 1 SN - 0950-382X, 0950-382X KW - Genetics Abstracts; Biochemistry Abstracts 2: Nucleic Acids; Microbiology Abstracts B: Bacteriology KW - Tryptophan KW - Translation KW - Adaptations KW - Cell cycle KW - non-coding RNA KW - Enzymes KW - Plasmids KW - Monomers KW - Recombination KW - Cell division KW - Indole KW - Gene regulation KW - Reviews KW - Escherichia coli KW - Tryptophan 2,3-dioxygenase KW - J 02320:Cell Biology KW - N 14830:RNA KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19563469?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Molecular+Microbiology&rft.atitle=Tryptophanase+in+sRNA+control+of+the+Escherichia+coli+cell+cycle&rft.au=Chattoraj%2C+Dhruba+K&rft.aulast=Chattoraj&rft.aufirst=Dhruba&rft.date=2007-01-01&rft.volume=63&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Molecular+Microbiology&rft.issn=0950382X&rft_id=info:doi/10.1111%2Fj.1365-2958.2006.05517.x LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - SuppNotes - Figures, 1; references, 15. N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Translation; Tryptophan; Adaptations; Cell cycle; non-coding RNA; Enzymes; Plasmids; Monomers; Recombination; Cell division; Indole; Reviews; Gene regulation; Tryptophan 2,3-dioxygenase; Escherichia coli DO - http://dx.doi.org/10.1111/j.1365-2958.2006.05517.x ER - TY - JOUR T1 - research paper: Severity of pulmonary hypertension during vaso-occlusive pain crisis and exercise in patients with sickle cell disease AN - 19561280; 7225054 AB - Pulmonary hypertension is associated with sudden death and is a risk factor for mortality in adult patients with sickle cell disease. The high mortality despite only mild-to-moderate increases in pulmonary vascular resistance remains an unresolved paradox. Accordingly, little is known about the cardiovascular effects of stressors, such as vaso-occlusive pain crisis (VOC) and exercise, which may acutely increase pulmonary pressures and impair right heart function. We therefore evaluated pulmonary artery pressures by echocardiogram in 25 patients with sickle cell disease in steady-state and during VOC, and by right heart catheterisation with exercise in a second cohort of 21 patients to determine whether pulmonary hypertension worsens during acute cardiopulmonary stress. TRV increased during VOC (P < 0.001), and the increased pulmonary pressures during VOC were associated with decreases in haemoglobin levels (P < 0.001), and increases in lactate dehydrogenase (P < 0.001) and plasma haemoglobin levels (P = 0.03). During exercise stress performed during cardiac catheterisation, mean pulmonary artery pressures (P < 0.001) and pulmonary vascular resistance increased (P < 0.001) in all subjects. These data suggest that acute elevations in pulmonary pressures during VOC or exercise may contribute to morbidity and mortality in patients with sickle cell disease. JF - British Journal of Haematology AU - Machado, Roberto F AU - Kyle Mack, A AU - Martyr, Sabrina AU - Barnett, Christopher AU - MacArthur, Peter AU - Sachdev, Vandana AU - Ernst, Inez AU - Hunter, Lori A AU - Coles, Wynona A AU - Nichols, James P AU - Kato, Gregory J AU - Gladwin, Mark T AD - Vascular Medicine Branch, National Heart Lung and Blood Institute Critical Care Medicine Department, Clinical Center Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 319 EP - 325 PB - Blackwell Publishing Ltd., 9600 Garsington Road Oxford OX4 2DQ UK, [URL:http://www.blackwellpublishing.com] VL - 136 IS - 2 SN - 0007-1048, 0007-1048 KW - Physical Education Index KW - Heart KW - Death KW - Exercise physiology KW - Stress KW - Pain KW - Patients KW - Adults KW - Risk factors KW - Lactic acid KW - Cardiorespiratory KW - Diseases KW - Circulatory system KW - Hypertension KW - PE 090:Sports Medicine & Exercise Sport Science UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19561280?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+Journal+of+Haematology&rft.atitle=research+paper%3A+Severity+of+pulmonary+hypertension+during+vaso-occlusive+pain+crisis+and+exercise+in+patients+with+sickle+cell+disease&rft.au=Machado%2C+Roberto+F%3BKyle+Mack%2C+A%3BMartyr%2C+Sabrina%3BBarnett%2C+Christopher%3BMacArthur%2C+Peter%3BSachdev%2C+Vandana%3BErnst%2C+Inez%3BHunter%2C+Lori+A%3BColes%2C+Wynona+A%3BNichols%2C+James+P%3BKato%2C+Gregory+J%3BGladwin%2C+Mark+T&rft.aulast=Machado&rft.aufirst=Roberto&rft.date=2007-01-01&rft.volume=136&rft.issue=2&rft.spage=319&rft.isbn=&rft.btitle=&rft.title=British+Journal+of+Haematology&rft.issn=00071048&rft_id=info:doi/10.1111%2Fj.1365-2141.2006.06417.x LA - English DB - Physical Education Index N1 - Date revised - 2007-05-01 N1 - SuppNotes - Figures, 2; tables, 1. N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Heart; Exercise physiology; Death; Stress; Patients; Pain; Adults; Risk factors; Lactic acid; Cardiorespiratory; Diseases; Hypertension; Circulatory system DO - http://dx.doi.org/10.1111/j.1365-2141.2006.06417.x ER - TY - JOUR T1 - An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti* AN - 19557997; 7273819 AB - Saliva of blood-sucking arthropods contains a cocktail of antihemostatic agents and immunomodulators that help blood feeding. Mosquitoes additionally feed on sugar meals and have specialized regions of their glands containing glycosidases and antimicrobials that might help control bacterial growth in the ingested meals. To expand our knowledge on the salivary cocktail of Aedes aegypti, a vector of dengue and yellow fevers, we analyzed a set of 4,232 expressed sequence tags from cDNA libraries of adult female mosquitoes. Results A nonredundant catalogue of 614 transcripts (573 of which are novel) is described, including 136 coding for proteins of a putative secretory nature. Additionally, a two-dimensional gel electrophoresis of salivary gland (SG) homogenates followed by tryptic digestion of selected protein bands and MS/MS analysis revealed the expression of 24 proteins. Analysis of tissue-specific transcription of a subset of these genes revealed at least 31 genes whose expression is specific or enriched in female SG, whereas 24 additional genes were expressed in female SG and in males but not in other female tissues. Most of the 55 proteins coded by these SG transcripts have no known function and represent high-priority candidates for expression and functional analysis as antihemostatic or antimicrobial agents. An unexpected finding is the occurrence of four protein families specific to SG that were probably a product of horizontal transfer from prokaryotic organisms to mosquitoes. Conclusion Overall, this paper contributes to the novel identification of 573 new transcripts, or near 3% of the Ae. aegypti proteome assuming a 20,000- protein set, and to the best-described sialome of any blood-feeding insect. JF - BMC Genomics AU - Ribeiro, Jose MC AU - Arca, Bruno AU - Lombardo, Fabrizio AU - Calvo, Eric AU - Phan, van My AU - Chandra, Prafulla K AU - Wikel, Stephen K AD - Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, Maryland 20852, USA, Jribeiro@niaid.nih.gov Y1 - 2007 PY - 2007 DA - 2007 PB - BioMed Central Ltd., Middlesex House 34-42 Cleveland Street London W1T 4LB UK, [mailto:info@biomedcentral.com], [URL:http://www.biomedcentral.com] VL - 8 SN - 1471-2164, 1471-2164 KW - Yellow fever mosquito KW - Virology & AIDS Abstracts; Microbiology Abstracts B: Bacteriology; Entomology Abstracts; Genetics Abstracts KW - Article No. 6 KW - Sugar KW - Feeding KW - Aedes aegypti KW - protein families KW - Transcription KW - Salivary gland KW - Immunomodulation KW - expressed sequence tags KW - Horizontal transfer KW - Gel electrophoresis KW - Antimicrobial agents KW - Blood KW - Arthropoda KW - Dengue KW - Yellow fever KW - Saliva KW - G 07810:Insects KW - Z 05360:Genetics and Evolution KW - J 02350:Immunology KW - V 22400:Human Diseases UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19557997?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+Genomics&rft.atitle=An+annotated+catalogue+of+salivary+gland+transcripts+in+the+adult+female+mosquito%2C+Aedes+aegypti*&rft.au=Ribeiro%2C+Jose+MC%3BArca%2C+Bruno%3BLombardo%2C+Fabrizio%3BCalvo%2C+Eric%3BPhan%2C+van+My%3BChandra%2C+Prafulla+K%3BWikel%2C+Stephen+K&rft.aulast=Ribeiro&rft.aufirst=Jose&rft.date=2007-01-01&rft.volume=8&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=BMC+Genomics&rft.issn=14712164&rft_id=info:doi/10.1186%2F1471-2164-8-6 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-06-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Feeding; Sugar; Transcription; protein families; Salivary gland; expressed sequence tags; Immunomodulation; Gel electrophoresis; Horizontal transfer; Antimicrobial agents; Blood; Dengue; Yellow fever; Saliva; Aedes aegypti; Arthropoda DO - http://dx.doi.org/10.1186/1471-2164-8-6 ER - TY - JOUR T1 - Characterizing the unfolded states of proteins using single-molecule FRET spectroscopy and molecular simulations AN - 19550260; 7255142 AB - To obtain quantitative information on the size and dynamics of unfolded proteins we combined single-molecule lifetime and intensity FRET measurements with molecular simulations. We compared the unfolded states of the 64-residue, alpha / beta protein L and the 66-residue, all- beta cold-shock protein CspTm. The average radius of gyration (R sub(g)) calculated from FRET data on freely diffusing molecules was identical for the two unfolded proteins at guanidinium chloride concentrations >3 M, and the FRET-derived R sub(g) of protein L agreed well with the R sub(g) previously measured by equilibrium small-angle x-ray scattering. As the denaturant concentration was lowered, the mean FRET efficiency of the unfolded subpopulation increased, signaling collapse of the polypeptide chain, with protein L being slightly more compact than CspTm. A decrease in R sub(g) with decreasing denaturant was also observed in all-atom molecular dynamics calculations in explicit water/urea solvent, and Langevin simulations of a simplified representation of the polypeptide suggest that collapse can result from either increased interresidue attraction or decreased excluded volume. In contrast to both the FRET and simulation results, previous time-resolved small-angle x-ray scattering experiments showed no collapse for protein L. Analysis of the donor fluorescence decay of the unfolded subpopulation of both proteins gives information about the end-to-end chain distribution and suggests that chain dynamics is slow compared with the donor life-time of approximately 2 ns, whereas the bin-size independence of the small excess width above the shot noise for the FRET efficiency distributions may result from incomplete conformational averaging on even the 1-ms time scale. JF - Proceedings of the National Academy of Sciences, USA AU - Merchant, Kusai A AU - Best, Robert B AU - Louis, John M AU - Gopich, Irina V AU - Eaton, William A AD - Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1528 EP - 1533 PB - National Academy of Sciences, 2101 Constitution Ave. Washington DC 20418 USA VL - 104 IS - 5 SN - 0027-8424, 0027-8424 KW - Biotechnology and Bioengineering Abstracts KW - Fluorescence KW - Subpopulations KW - Solvents KW - fluorescence resonance energy transfer KW - Chloride KW - Urea KW - Spectroscopy KW - protein L KW - Protein folding KW - X-ray scattering KW - Cold shock KW - Signal transduction KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19550260?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.atitle=Characterizing+the+unfolded+states+of+proteins+using+single-molecule+FRET+spectroscopy+and+molecular+simulations&rft.au=Merchant%2C+Kusai+A%3BBest%2C+Robert+B%3BLouis%2C+John+M%3BGopich%2C+Irina+V%3BEaton%2C+William+A&rft.aulast=Merchant&rft.aufirst=Kusai&rft.date=2007-01-01&rft.volume=104&rft.issue=5&rft.spage=1528&rft.isbn=&rft.btitle=&rft.title=Proceedings+of+the+National+Academy+of+Sciences%2C+USA&rft.issn=00278424&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - fluorescence resonance energy transfer; protein L; Protein folding; X-ray scattering; Subpopulations; Urea; Solvents; Signal transduction; Fluorescence; Spectroscopy; Chloride; Cold shock ER - TY - JOUR T1 - GenBank AN - 19543782; 7254402 AB - GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 240 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage (www.ncbi.nlm.nih.gov). JF - Nucleic Acids Research AU - Benson, Dennis A AU - Karsch-Mizrachi, Ilene AU - Lipman, David J AU - Ostell, James AU - Wheeler, David L AD - National Center for Biotechnology Information, National Library of Medicine National Institutes of Health, Building 38A, 8600 Rockville Pike, Bethesda, MD 20894, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D21 EP - D25 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Peptide mapping KW - Nucleotide sequence KW - Protein structure KW - Data banks KW - Computer programs KW - Databases KW - DNA KW - Taxonomy KW - Amino acid sequence KW - Gene mapping KW - N 14845:Miscellaneous KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19543782?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=GenBank&rft.au=Benson%2C+Dennis+A%3BKarsch-Mizrachi%2C+Ilene%3BLipman%2C+David+J%3BOstell%2C+James%3BWheeler%2C+David+L&rft.aulast=Benson&rft.aufirst=Dennis&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D21&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Databases; Nucleotide sequence; DNA; Data banks; Peptide mapping; Computer programs; Genomes; Gene mapping; Taxonomy; Protein structure; Amino acid sequence ER - TY - JOUR T1 - Database resources of the National Center for Biotechnology Information AN - 19543673; 7254485 AB - In addition to maintaining the GenBank super( registered ) nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data made available through NCBI's Web site. NCBI resources include Entrez, the Entrez Programming Utilities, My NCBI, PubMed, PubMed Central, Entrez Gene, the NCBI Taxonomy Browser, BLAST, BLAST Link(BLink), Electronic PCR, OrfFinder, Spidey, Splign, RefSeq, UniGene, HomoloGene, ProtEST, dbMHC, dbSNP, Cancer Chromosomes, Entrez Genome, Genome Project and related tools, the Trace and Assembly Archives, the Map Viewer, Model Maker, Evidence Viewer, Clusters of Orthologous Groups (COGs), Viral Genotyping Tools, Influenza Viral Resources, HIV-1/Human Protein Interaction Database, Gene Expression Omnibus (GEO), Entrez Probe, GENSAT, Online Mendelian Inheritance in Man (OMIM), Online Mendelian Inheritance in Animals (OMIA), the Molecular Modeling Database (MMDB), the Conserved Domain Database (CDD), the Conserved Domain Architecture Retrieval Tool (CDART) and the PubChem suite of small molecule databases. Augmenting many of the Web applications are custom implementations of the BLAST program optimized to search specialized data sets. These resources can be accessed through the NCBI home page at www.ncbi.nlm.nih.gov. JF - Nucleic Acids Research AU - Wheeler, David L AU - Barrett, Tanya AU - Benson, Dennis A AU - Bryant, Stephen H AU - Canese, Kathi AU - Chetvernin, Vyacheslav AU - Church, Deanna M AU - DiCuccio, Michael AU - Edgar, Ron AU - Federhen, Scott AU - Geer, Lewis Y AU - Kapustin, Yuri AU - Khovayko, Oleg AU - Landsman, David AU - Lipman, David J AU - Madden, Thomas L AU - Maglott, Donna R AU - Ostell, James AU - Miller, Vadim AU - Pruitt, Kim D AU - Schuler, Gregory D AU - Sequeira, Edwin AU - Sherry, Steven T AU - Sirotkin, Karl AU - Souvorov, Alexandre AU - Starchenko, Grigory AU - Tatusov, Roman L AU - Tatusova, Tatiana A AU - Wagner, Lukas AU - Yaschenko, Eugene AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Building 38A, 8600 Rockville Pike, Bethesda, MD 20894, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D5 EP - D12 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Genomes KW - Molecular modelling KW - Heredity KW - DNA probes KW - Gene expression KW - Influenza KW - Computer programs KW - Chromosomes KW - Polymerase chain reaction KW - Genotyping KW - Cancer KW - Databases KW - nucleic acids KW - Taxonomy KW - Protein interaction KW - N 14810:Methods KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19543673?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=Database+resources+of+the+National+Center+for+Biotechnology+Information&rft.au=Wheeler%2C+David+L%3BBarrett%2C+Tanya%3BBenson%2C+Dennis+A%3BBryant%2C+Stephen+H%3BCanese%2C+Kathi%3BChetvernin%2C+Vyacheslav%3BChurch%2C+Deanna+M%3BDiCuccio%2C+Michael%3BEdgar%2C+Ron%3BFederhen%2C+Scott%3BGeer%2C+Lewis+Y%3BKapustin%2C+Yuri%3BKhovayko%2C+Oleg%3BLandsman%2C+David%3BLipman%2C+David+J%3BMadden%2C+Thomas+L%3BMaglott%2C+Donna+R%3BOstell%2C+James%3BMiller%2C+Vadim%3BPruitt%2C+Kim+D%3BSchuler%2C+Gregory+D%3BSequeira%2C+Edwin%3BSherry%2C+Steven+T%3BSirotkin%2C+Karl%3BSouvorov%2C+Alexandre%3BStarchenko%2C+Grigory%3BTatusov%2C+Roman+L%3BTatusova%2C+Tatiana+A%3BWagner%2C+Lukas%3BYaschenko%2C+Eugene&rft.aulast=Wheeler&rft.aufirst=David&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D5&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Databases; Heredity; Molecular modelling; Genomes; Influenza; Computer programs; Genotyping; Protein interaction; Gene expression; Chromosomes; Cancer; nucleic acids; Taxonomy; DNA probes; Polymerase chain reaction ER - TY - JOUR T1 - Oral Vaccination with Salmonella Simultaneously Expressing Yersinia pestis F1 and V Antigens Protects against Bubonic and Pneumonic Plague AN - 19543631; 7253289 AB - The gut provides a large area for immunization enabling the development of mucosal and systemic Ab responses. To test whether the protective Ags to Yersinia pestis can be orally delivered, the Y. pestis caf1 operon, encoding the F1-Ag and virulence Ag (V-Ag) were cloned into attenuated Salmonella vaccine vectors. F1-Ag expression was controlled under a promoter from the caf1 operon; two different promoters (P), PtetA in pV3, PphoP in pV4, as well as a chimera of the two in pV55 were tested. F1-Ag was amply expressed; the chimera in the pV55 showed the best V-Ag expression. Oral immunization with Salmonella-F1 elicited elevated secretory (S)-IgA and serum IgG titers, and Salmonella-V-Ag(pV55) elicited much greater S-IgA and serum IgG Ab titers than Salmonella-V-Ag(pV3) or Salmonella-V-Ag(pV4). Hence, a new Salmonella vaccine, Salmonella-(F1+V)Ags, made with a single plasmid containing the caf1 operon and the chimeric promoter for V-Ag allowed the simultaneous expression of F1 capsule and V-Ag. Salmonella-(F1+V)Ags elicited elevated Ab titers similar to their monotypic derivatives. For bubonic plague, mice dosed with Salmonella-(F1+V)Ags and Salmonella-F1-Ag showed similar efficacy (>83% survival) against similar to 1000 LD sub(50) Y. pestis. For pneumonic plague, immunized mice required immunity to both F1- and V-Ags because the mice vaccinated with Salmonella-(F1+V)Ags protected against 100 LD sub(50) Y. pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis. JF - Journal of Immunology AU - Yang, Xinghong AU - Hinnebusch, BJoseph AU - Trunkle, Theresa AU - Bosio, Catharine M AU - Suo, Zhiyong AU - Tighe, Mike AU - Harmsen, Ann AU - Becker, Todd AU - Crist, Kathryn AU - Walters, Nancy AU - Avci, Recep AU - Pascual, David W AD - Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717. Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80521. Physics Department, Montana State University, Bozeman, MT 59717 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1059 EP - 1067 PB - American Association of Immunologists, 9650 Rockville Pike Bethesda MD 20814-3998 USA, [URL:http://www.jimmunol.org/] VL - 178 IS - 2 SN - 0022-1767, 0022-1767 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - Mucosal immunity KW - Yersinia pestis KW - Survival KW - Immunity KW - Plasmids KW - Vaccination KW - Virulence KW - Chimeras KW - Promoters KW - Digestive tract KW - Immunoglobulin G KW - Vaccines KW - Plague KW - Operons KW - Salmonella KW - F 06905:Vaccines KW - J 02350:Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19543631?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunology&rft.atitle=Oral+Vaccination+with+Salmonella+Simultaneously+Expressing+Yersinia+pestis+F1+and+V+Antigens+Protects+against+Bubonic+and+Pneumonic+Plague&rft.au=Yang%2C+Xinghong%3BHinnebusch%2C+BJoseph%3BTrunkle%2C+Theresa%3BBosio%2C+Catharine+M%3BSuo%2C+Zhiyong%3BTighe%2C+Mike%3BHarmsen%2C+Ann%3BBecker%2C+Todd%3BCrist%2C+Kathryn%3BWalters%2C+Nancy%3BAvci%2C+Recep%3BPascual%2C+David+W&rft.aulast=Yang&rft.aufirst=Xinghong&rft.date=2007-01-01&rft.volume=178&rft.issue=2&rft.spage=1059&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunology&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Mucosal immunity; Survival; Immunity; Plasmids; Vaccination; Virulence; Promoters; Chimeras; Digestive tract; Immunoglobulin G; Plague; Vaccines; Operons; Yersinia pestis; Salmonella ER - TY - JOUR T1 - Association of Physical Activity with Development of Uterine Leiomyoma AN - 19537969; 7250303 AB - The relation between physical activity and uterine leiomyomata (fibroids) has received little study, but exercise is protective for breast cancer, another hormonally mediated tumor. Participants in this study were randomly selected members of a health plan based in Washington, DC, aged 35-49 years (734 African Americans, 455 Whites) enrolled between 1996 and 1999. Fibroid status was based on ultrasound screening. Physical activity was based on detailed interview questions. Logistic regression with adjustment for body mass index and other risk factors showed that women in the highest category of physical activity were significantly less likely to have fibroids (odds ratio = 0.6, 95% confidence interval = 0.4, 0.9 for the highest vs. the lowest category (equivalent to approximately greater than or equal to 7 hours/week vs. <2 hours/week)). There was a dose-response pattern; a significant trend was seen for both African-American and White women. A multistate Bayesian analysis indicated that exercise was associated with tumor onset more strongly than with tumor growth. When data for women who reported major fibroid-related symptoms were excluded, results remained essentially unchanged, suggesting that the observed association could not be attributed to reverse causation (fibroids preventing exercise). The authors concluded that regular exercise might help women prevent fibroids. JF - American Journal of Epidemiology AU - Baird, Donna Day AU - Dunson, David B AU - Hill, Michael C AU - Cousins, Deborah AU - Schectman, Joel M AD - Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 157 EP - 163 PB - Oxford University Press, Oxford Journals Health, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 165 IS - 2 SN - 0002-9262, 0002-9262 KW - Physical Education Index KW - Blacks KW - Preventive health KW - Body mass KW - Women KW - Breasts KW - Tumors KW - Exercise KW - Cancer KW - Epidemiology KW - Risk factors KW - Analysis KW - Interviews KW - Trends KW - Ultrasound KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19537969?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Epidemiology&rft.atitle=Association+of+Physical+Activity+with+Development+of+Uterine+Leiomyoma&rft.au=Baird%2C+Donna+Day%3BDunson%2C+David+B%3BHill%2C+Michael+C%3BCousins%2C+Deborah%3BSchectman%2C+Joel+M&rft.aulast=Baird&rft.aufirst=Donna&rft.date=2007-01-01&rft.volume=165&rft.issue=2&rft.spage=157&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Preventive health; Blacks; Body mass; Women; Breasts; Exercise; Tumors; Cancer; Epidemiology; Analysis; Risk factors; Interviews; Trends; Ultrasound ER - TY - JOUR T1 - Lung injury after ozone exposure is iron dependent AN - 19532826; 7250243 AB - We tested the hypothesis that oxidative stress and biological effect after ozone (O sub(3)) exposure are dependent on changes in iron homeostasis. After O sub(3) exposure, healthy volunteers demonstrated increased lavage concentrations of iron, transferrin, lactoferrin, and ferritin. In normal rats, alterations of iron metabolism after O sub(3) exposure were immediate and preceded the inflammatory influx. To test for participation of this disruption in iron homeostasis in lung injury following O sub(3) inhalation, we exposed Belgrade rats, which are functionally deficient in divalent metal transporter 1 (DMT1) as a means of iron uptake, and controls to O sub(3). Iron homeostasis was disrupted to a greater extent and the extent of injury was greater in Belgrade rats than in control rats. Nonheme iron and ferritin concentrations were higher in human bronchial epithelial (HBE) cells exposed to O sub(3) than in HBE cells exposed to filtered air. Aldehyde generation and IL-8 release by the HBE cells was also elevated following O sub(3) exposure. Human embryonic kidney (HEK 293) cells with elevated expression of a DMT1 construct were exposed to filtered air and O sub(3). With exposure to O sub(3), elevated DMT1 expression diminished oxidative stress (i.e., aldehyde generation) and IL-8 release. We conclude that iron participates critically in the oxidative stress and biological effects after O sub(3) exposure. JF - American Journal of Physiology: Lung Cellular and Molecular Physiology AU - Ghio, Andrew J AU - Turi, Jennifer L AU - Madden, Michael C AU - Dailey, Lisa A AU - Richards, Judy D AU - Stonehuerner, Jacqueline G AU - Morgan, Daniel L AU - Singleton, Steven AU - Garrick, Laura M AU - Garrick, Michael D AD - National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, and National Institute of Environmental Health Sciences and National Toxicology Program, Research Triangle Park Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - L134 EP - L143 PB - American Physiological Society, 9650 Rockville Pike Bethesda MD 20814-3991 USA, [mailto:webmaster@the-aps.org], [URL:http://www.the-aps.org/] VL - 292 IS - 1 SN - 1040-0605, 1040-0605 KW - Toxicology Abstracts KW - Inhalation KW - Injuries KW - Homeostasis KW - Interleukin 8 KW - Inflammation KW - Divalent metal transporter-1 KW - Transferrin KW - Lung KW - Oxidative stress KW - lactoferrin KW - Embryos KW - Ferritin KW - Aldehydes KW - Iron KW - Metabolism KW - Ozone KW - X 24360:Metals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19532826?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Physiology%3A+Lung+Cellular+and+Molecular+Physiology&rft.atitle=Lung+injury+after+ozone+exposure+is+iron+dependent&rft.au=Ghio%2C+Andrew+J%3BTuri%2C+Jennifer+L%3BMadden%2C+Michael+C%3BDailey%2C+Lisa+A%3BRichards%2C+Judy+D%3BStonehuerner%2C+Jacqueline+G%3BMorgan%2C+Daniel+L%3BSingleton%2C+Steven%3BGarrick%2C+Laura+M%3BGarrick%2C+Michael+D&rft.aulast=Ghio&rft.aufirst=Andrew&rft.date=2007-01-01&rft.volume=292&rft.issue=1&rft.spage=L134&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Physiology%3A+Lung+Cellular+and+Molecular+Physiology&rft.issn=10400605&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-03-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Inhalation; Injuries; Homeostasis; Interleukin 8; Inflammation; Transferrin; Divalent metal transporter-1; Oxidative stress; Lung; lactoferrin; Ferritin; Embryos; Aldehydes; Iron; Metabolism; Ozone ER - TY - JOUR T1 - Can Biomarkers Help Us Understand the Nutritional and Lifestyle Factors Important in Cancer Prognosis? AN - 19519405; 7210034 AB - In attempting to discover the causes of cancer, investigators have recognized that biomarkers can confirm biological plausibility, enhance relative risks, and serve as surrogate endpoints in observational and intervention studies. In the arena of cancer survival, the potential value of biomarkers is increasingly appreciated. A broad range of histological, cellular, and molecular markers have been identified among persons diagnosed with cancer. Molecular and cellular markers are being used to stage disease, predict prognosis, and target therapeutic interventions. Biomarkers in survivors can also help us to understand factors that influence prognosis by both elucidating pertinent biological pathways and sharpening risk estimates. However, as in the case of incident cancer, the use of biomarkers as surrogate endpoints postdiagnosis is problematic because of the potential existence of alternative pathways to recurrence and death that bypass the surrogate endpoint. In evaluating potential surrogates, an understanding of the causal structure underlying the interrelations of exposures, surrogate, and recurrence or death is essential. Three questions can help to shed light on this structure: 1) What is the relation of the surrogate endpoint to recurrence or death? 2) What is the relation of the intervention (or exposure) to the surrogate? 3) To what extent does the surrogate endpoint mediate the relation between intervention (exposure) and recurrence or death? To address these questions, it is imperative to integrate biomarker studies into ongoing pharmacotherapeutic and lifestyle intervention studies with recurrence or mortality as explicit endpoints. JF - Journal of Nutrition AU - Schatzkin, Arthur AD - Nutritional Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD 20815 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 249S EP - 252S PB - American Society for Nutritional Sciences, 9650 Rockville Pike, Room L-2407A Bethesda MD 20814 USA, [mailto:staff@faseb.org], [URL:http://www.nutrition.org] VL - 137 IS - 1 SN - 0022-3166, 0022-3166 KW - Risk Abstracts KW - Bioindicators KW - Mortality KW - intervention KW - survival KW - Nutrition KW - Cancer KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19519405?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Nutrition&rft.atitle=Can+Biomarkers+Help+Us+Understand+the+Nutritional+and+Lifestyle+Factors+Important+in+Cancer+Prognosis%3F&rft.au=Schatzkin%2C+Arthur&rft.aulast=Schatzkin&rft.aufirst=Arthur&rft.date=2007-01-01&rft.volume=137&rft.issue=1&rft.spage=249S&rft.isbn=&rft.btitle=&rft.title=Journal+of+Nutrition&rft.issn=00223166&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Bioindicators; Mortality; intervention; survival; Nutrition; Cancer ER - TY - JOUR T1 - Binge Eating in Overweight Treatment-Seeking Adolescents AN - 19518936; 7209364 AB - OBJECTIVE: To examine the frequency and recency of binge eating in relation to psychopathology in overweight, treatment-seeking adolescents. METHODS: We investigated psychological correlates of the frequency and recency of reported loss of control (LOC) eating episodes in 160 overweight (body mass index [BMI]: 40.7 plus or minus 8.8 kg/m super(2)) adolescents. On the basis of the responses to the eating disorder examination (EDE), participants were categorized into one of four groups: full-syndrome binge eating disorder (BED); recent but infrequent binge eating (episodes within the 3 months before interview; RECENT-BINGE); remote and infrequent LOC eating (episodes occurring >3 months before assessment; PAST-LOC), or no history of LOC episodes (NE). RESULTS: The BED group reported higher EDE scores (global, p < .01), and more negative mood and anxiety than all other groups (p's < .01). Compared with NE, RECENT-BINGE also reported more anxiety and higher EDE scores (p's < .01). CONCLUSIONS: Overweight, treatment-seeking adolescents with BED are clearly distinguishable from teens without the disorder on measures of eating-related psychopathology, mood, and anxiety. RECENT-BINGE, but not PAST-LOC, is also associated with significantly greater eating-related and general psychopathology. JF - Journal of Pediatric Psychology AU - Glasofer, Deborah R AU - Tanofsky-Kraff, Marian AU - Eddy, Kamryn T AU - Yanovski, Susan Z AU - Theim, Kelly R AU - Mirch, Margaret C AU - Ghorbani, Samareh AU - Ranzenhofer, Lisa M AU - Haaga, David AU - Yanovski, Jack A AD - Unit on Growth and Obesity, Developmental Endocrinology Branch, National Institute of Child Health and Human Development. Department of Psychology, American University. Center for Anxiety and Related Disorders, Boston University. Optimal Weight for Life Clinic, Children's Hospital. Division of Digestive Diseases and Nutrition, and. Division of Nutrition Research Coordination, National Institute of Diabetes and Digestive Kidney Diseases, National Institutes of Health, DHHS Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 95 EP - 105 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 32 IS - 1 SN - 0146-8693, 0146-8693 KW - Physical Education Index KW - Obesity KW - Anxiety KW - Eating disorders KW - Psychology KW - Pediatrics KW - Adolescence KW - Body mass KW - Evaluation KW - Moods KW - Diet KW - Interviews KW - Youth KW - PE 030:Exercise, Health & Physical Fitness UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19518936?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aphysicaleducation&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Pediatric+Psychology&rft.atitle=Binge+Eating+in+Overweight+Treatment-Seeking+Adolescents&rft.au=Glasofer%2C+Deborah+R%3BTanofsky-Kraff%2C+Marian%3BEddy%2C+Kamryn+T%3BYanovski%2C+Susan+Z%3BTheim%2C+Kelly+R%3BMirch%2C+Margaret+C%3BGhorbani%2C+Samareh%3BRanzenhofer%2C+Lisa+M%3BHaaga%2C+David%3BYanovski%2C+Jack+A&rft.aulast=Glasofer&rft.aufirst=Deborah&rft.date=2007-01-01&rft.volume=32&rft.issue=1&rft.spage=95&rft.isbn=&rft.btitle=&rft.title=Journal+of+Pediatric+Psychology&rft.issn=01468693&rft_id=info:doi/ LA - English DB - Physical Education Index N1 - Date revised - 2007-06-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Evaluation; Obesity; Anxiety; Pediatrics; Psychology; Eating disorders; Body mass; Adolescence; Moods; Interviews; Diet; Youth ER - TY - JOUR T1 - Analysis and prediction of functionally important sites in proteins AN - 19517972; 7210595 AB - The rapidly increasing volume of sequence and structure information available for proteins poses the daunting task of determining their functional importance. Computational methods can prove to be very useful in understanding and characterizing the biochemical and evolutionary information contained in this wealth of data, particularly at functionally important sites. Therefore, we perform a detailed survey of compositional and evolutionary constraints at the molecular and biological function level for a large set of known functionally important sites extracted from a wide range of protein families. We compare the degree of conservation across different functional categories and provide detailed statistical insight to decipher the varying evolutionary constraints at functionally important sites. The compositional and evolutionary information at functionally important sites has been compiled into a library of functional templates. We developed a module that predicts functionally important columns (FIC) of an alignment based on the detection of a significant "template match score" to a library template. Our template match score measures an alignment column's similarity to a library template and combines a term explicitly representing a column's residue composition with various evolutionary conservation scores (information content and position-specific scoring matrix-derived statistics). Our benchmarking studies show good sensitivity/specificity for the prediction of functional sites and high accuracy in attributing correct molecular function type to the predicted sites. This prediction method is based on information derived from homologous sequences and no structural information is required. Therefore, this method could be extremely useful for large-scale functional annotation. JF - Protein Science AU - Chakrabarti, Saikat AU - Lanczycki, Christopher J AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 4 EP - 13 PB - Cold Spring Harbor Laboratory Press, [mailto:cshpress@cshl.org], [URL:http://www.cshl.org/] VL - 16 IS - 1 SN - 0961-8368, 0961-8368 KW - Biotechnology and Bioengineering Abstracts KW - Evolutionary conservation KW - Statistical analysis KW - protein families KW - Computer applications KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19517972?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Protein+Science&rft.atitle=Analysis+and+prediction+of+functionally+important+sites+in+proteins&rft.au=Chakrabarti%2C+Saikat%3BLanczycki%2C+Christopher+J&rft.aulast=Chakrabarti&rft.aufirst=Saikat&rft.date=2007-01-01&rft.volume=16&rft.issue=1&rft.spage=4&rft.isbn=&rft.btitle=&rft.title=Protein+Science&rft.issn=09618368&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-07-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Evolutionary conservation; Statistical analysis; protein families; Computer applications ER - TY - JOUR T1 - CYP2E1 AN - 19517315; 7207219 AB - Bernard B. Brodie's laboratory was the first to examine the mechanisms of drug-induced toxicity at the molecular level. They found that acetaminophen hepatotoxicity was due to the metabolic activation of the drug to a highly reactive toxic metabolite that depleted cellular glutathione and covalently bound to protein. Subsequent studies revealed that activation of acetaminophen to an active metabolite is primarily carried out by CYP2E1, an ethanol-inducible cytochrome P450 that was first suggested by characterization of the microsomal ethanol oxidation system. CYP2E1 is developmentally regulated, under liver-specific control, and undergoes substrate-induced protein stabilization. It is also regulated by starvation and diabetes through insulin-dependent mRNA stabilization. In addition to acetaminophen, CYP2E1 metabolically activates a large number of low M sub(r) toxicants and carcinogens and thus is of great toxicological importance. The mechanism of regulation CYP2E1 and its role in acetaminophen toxicity will be discussed. JF - Drug Metabolism and Disposition AU - Gonzalez, Frank J AD - Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 1 EP - 8 PB - Williams & Wilkins, 351 W. Camden St. Baltimore MD 21201 USA, [URL:http://www.lww.com/] VL - 35 IS - 1 SN - 0090-9556, 0090-9556 KW - Toxicology Abstracts KW - Starvation KW - Toxicants KW - Glutathione KW - Metabolites KW - Carcinogens KW - Toxicity KW - Drug abuse KW - hepatotoxicity KW - mRNA KW - Diabetes mellitus KW - Oxidation KW - Metabolic activation KW - Cytochrome P450 KW - Acetaminophen KW - Ethanol KW - X 24310:Pharmaceuticals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19517315?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Drug+Metabolism+and+Disposition&rft.atitle=CYP2E1&rft.au=Gonzalez%2C+Frank+J&rft.aulast=Gonzalez&rft.aufirst=Frank&rft.date=2007-01-01&rft.volume=35&rft.issue=1&rft.spage=1&rft.isbn=&rft.btitle=&rft.title=Drug+Metabolism+and+Disposition&rft.issn=00909556&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Starvation; Toxicants; Glutathione; Metabolites; Toxicity; Carcinogens; Drug abuse; hepatotoxicity; mRNA; Diabetes mellitus; Oxidation; Metabolic activation; Cytochrome P450; Acetaminophen; Ethanol ER - TY - JOUR T1 - Cerebrospinal Fluid-Infiltrating CD4 super(+) T Cells Recognize Borrelia burgdorferi Lysine-Enriched Protein Domains and Central Nervous System Autoantigens in Early Lyme Encephalitis AN - 19516097; 7207598 AB - Neurological manifestations of Lyme disease are usually accompanied by inflammatory changes in the cerebrospinal fluid (CSF) and the recruitment of activated T cells into the CSF compartment. In order to characterize the phenotype and identify target antigens of CSF-infiltrating T cells in early neuroborreliosis with central nervous system (CNS) involvement, we combined T-cell cloning, functional testing of T-cell responses with positional scanning synthetic combinatorial peptide libraries, and biometric data analysis. We demonstrate that CD4 super(+) gamma interferon-producing T cells specifically responding to Borrelia burgdorferi lysate were present in the CSF of a patient with acute Lyme encephalitis. Some T-cell clones recognized previously uncharacterized B. burgdorferi epitopes which show a specific enrichment for lysine, such as the heat shock-induced chaperone HSP90. Degenerate T-cell recognition that included T-cell responses to borrelia-specific and CNS-specific autoantigens derived from the myelin protein 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) could be demonstrated for one representative clone. Our results show that spirochetal antigen-specific and Th1-polarized CD4 super(+) lymphocytes infiltrate the CSF during monophasic CNS symptoms of Lyme disease and demonstrate that cross-recognition of CNS antigens by B. burgdorferi-specific T cells is not restricted to chronic and treatment-resistant manifestations. JF - Infection and Immunity AU - Luenemann, Jan D AU - Gelderblom, Harald AU - Sospedra, Mireia AU - Quandt, Jacqueline A AU - Pinilla, Clemencia AU - Marques, Adriana AU - Martin, Roland AD - Neuroimmunology Branch, Cellular Immunology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892. Institute for Neuroimmunology and Clinical MS Research (INiMS), Center for Molecular Neurobiology Hamburg (ZMNH), University Clinic Eppendorf, Falkenried 94, 20251 Hamburg, Germany. Mixture Science and Torrey Pines Institute for Molecular Studies, San Diego, California 92121. Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892. Laboratory of Viral Immunobiology, The Rockefeller University, New York, New York 10021. Department of Neurology and Psychiatry, Charite Medical Center, Humboldt University, 10098 Berlin, Germany Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 243 EP - 251 PB - American Society for Microbiology, 1752 N Street N.W. Washington, DC 20036 USA, [URL:http://www.asm.org/] VL - 75 IS - 1 SN - 0019-9567, 0019-9567 KW - Immunology Abstracts; Microbiology Abstracts B: Bacteriology; CSA Neurosciences Abstracts KW - Central nervous system KW - Data processing KW - Myelin KW - Borrelia burgdorferi KW - Lymphocytes B KW - Lysine KW - Biometrics KW - Peptide libraries KW - Encephalitis KW - Autoantigens KW - Nucleotides KW - Inflammation KW - Hsp90 protein KW - CD4 antigen KW - Cerebrospinal fluid KW - Scanning KW - Heat KW - Borreliosis KW - Lymphocytes T KW - Chaperones KW - Epitopes KW - Lyme disease KW - J 02350:Immunology KW - F 06910:Microorganisms & Parasites KW - N3 11024:Neuroimmunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19516097?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Infection+and+Immunity&rft.atitle=Cerebrospinal+Fluid-Infiltrating+CD4+super%28%2B%29+T+Cells+Recognize+Borrelia+burgdorferi+Lysine-Enriched+Protein+Domains+and+Central+Nervous+System+Autoantigens+in+Early+Lyme+Encephalitis&rft.au=Luenemann%2C+Jan+D%3BGelderblom%2C+Harald%3BSospedra%2C+Mireia%3BQuandt%2C+Jacqueline+A%3BPinilla%2C+Clemencia%3BMarques%2C+Adriana%3BMartin%2C+Roland&rft.aulast=Luenemann&rft.aufirst=Jan&rft.date=2007-01-01&rft.volume=75&rft.issue=1&rft.spage=243&rft.isbn=&rft.btitle=&rft.title=Infection+and+Immunity&rft.issn=00199567&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Central nervous system; Data processing; Myelin; Lymphocytes B; Lysine; Biometrics; Peptide libraries; Nucleotides; Autoantigens; Encephalitis; Inflammation; Hsp90 protein; Cerebrospinal fluid; CD4 antigen; Scanning; Heat; Borreliosis; Lymphocytes T; Chaperones; Epitopes; Lyme disease; Borrelia burgdorferi ER - TY - JOUR T1 - Inhalational Exposure to Carbonyl Sulfide Produces Altered Brainstem Auditory and Somatosensory-Evoked Potentials in Fischer 344N Rats AN - 19515662; 7210974 AB - Carbonyl sulfide (COS), a chemical listed by the original Clean Air Act, was tested for neurotoxicity by a National Institute of Environmental Health Sciences/National Toxicology Program and U.S. Environmental Protection Agency collaborative investigation. Previous studies demonstrated that COS produced cortical and brainstem lesions and altered auditory neurophysiological responses to click stimuli. This paper reports the results of expanded neurophysiological examinations that were an integral part of the previously published experiments (Morgan et al., 2004, TOXICOL: Appl. Pharmacol. 200, 131-145; Sills et al., 2004, TOXICOL: Pathol. 32, 1-10). Fisher 334N rats were exposed to 0, 200, 300, or 400 ppm COS for 6 h/day, 5 days/week for 12 weeks, or to 0, 300, or 400 ppm COS for 2 weeks using whole-body inhalation chambers. After treatment, the animals were studied using neurophysiological tests to examine: peripheral nerve function, somatosensory-evoked potentials (SEPs) (tail/hindlimb and facial cortical regions), brainstem auditory-evoked responses (BAERs), and visual flash-evoked potentials (2-week study). Additionally, the animals exposed for 2 weeks were examined using a functional observational battery (FOB) and response modification audiometry (RMA). Peripheral nerve function was not altered for any exposure scenario. Likewise, amplitudes of SEPs recorded from the cerebellum were not altered by treatment with COS. In contrast, amplitudes and latencies of SEPs recorded from cortical areas were altered after 12-week exposure to 400 ppm COS. The SEP waveforms were changed to a greater extent after forelimb stimulation than tail stimulation in the 2-week study. The most consistent findings were decreased amplitudes of BAER peaks associated with brainstem regions after exposure to 400 ppm COS. Additional BAER peaks were affected after 12 weeks, compared to 2 weeks of treatment, indicating that additional regions of the brainstem were damaged with longer exposures. The changes in BAERs were observed in the absence of altered auditory responsiveness in FOB or RMA. This series of experiments demonstrates that COS produces changes in brainstem auditory and cortical somatosensory neurophysiological responses that correlate with previously described histopathological damage. JF - Toxicological Sciences AU - Herr, David W AU - Graff, Jaimie E AU - Moser, Virginia C AU - Crofton, Kevin M AU - Little, Peter B AU - Morgan, Daniel L AU - Sills, Robert C AD - Neurotoxicology Division, MD B105-05, NHEERL, ORD, USEPA, Research Triangle Park, North Carolina 27711. Pathology Associates Division of Charles River Laboratories, Durham, North Carolina 27713. Respiratory Toxicology, NIEHS, Research Triangle Park, North Carolina 27709. Laboratory of Experimental Pathology, NIEHS, Research Triangle Park, North Carolina 27709 Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 118 EP - 135 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 95 IS - 1 SN - 1096-6080, 1096-6080 KW - Health & Safety Science Abstracts; CSA Neurosciences Abstracts; Toxicology Abstracts KW - Inhalation KW - Cerebellum KW - Environmental health KW - Histopathology KW - Rats KW - Clean Air Act KW - Somatosensory evoked potentials KW - Cortex KW - Batteries KW - Lesions KW - carbonyl compounds KW - Toxicology KW - Tails KW - Sulfides KW - Brain stem KW - EPA KW - Sulfide KW - USA KW - Neurotoxicity KW - carbonyls KW - Peripheral nerves KW - N3 11028:Neuropharmacology & toxicology KW - H 12000:Epidemiology and Public Health KW - X 24350:Industrial Chemicals UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19515662?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Toxicological+Sciences&rft.atitle=Inhalational+Exposure+to+Carbonyl+Sulfide+Produces+Altered+Brainstem+Auditory+and+Somatosensory-Evoked+Potentials+in+Fischer+344N+Rats&rft.au=Herr%2C+David+W%3BGraff%2C+Jaimie+E%3BMoser%2C+Virginia+C%3BCrofton%2C+Kevin+M%3BLittle%2C+Peter+B%3BMorgan%2C+Daniel+L%3BSills%2C+Robert+C&rft.aulast=Herr&rft.aufirst=David&rft.date=2007-01-01&rft.volume=95&rft.issue=1&rft.spage=118&rft.isbn=&rft.btitle=&rft.title=Toxicological+Sciences&rft.issn=10966080&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Inhalation; Somatosensory evoked potentials; Sulfide; Cortex; Batteries; Tails; Neurotoxicity; Brain stem; Cerebellum; carbonyls; Peripheral nerves; Clean Air Act; Rats; EPA; Sulfides; Lesions; Histopathology; Environmental health; carbonyl compounds; Toxicology; USA ER - TY - JOUR T1 - Cisplatin-DNA damage in p21 super(WAF1/Cip1) deficient mouse keratinocytes exposed to cisplatin AN - 19510108; 7209854 AB - In response to DNA damage, cell cycle arrest, apoptosis, and DNA repair are mediated by a TP53 pathway that induces p21 super(WAF1/Cip1). The chemotherapeutic drug cis-diamminedichloroplatinum-II (cisplatin) damages cellular DNA by forming cis-diammineplatinum-N super(7)-d[GpG] and cis-diammine-platinum-N super(7)-d[ApG] adducts. To investigate the role of p21, skin keratinocytes from p21 super(WAF1/Cip1) wild-type (+/+), heterozygous (+/-), and null (-/-) mice, cultured in calcium levels designed to maintain a proliferating state, were exposed to 5 mu M cisplatin continuously for 0, 8, 24, 48 and 72 h. At all time points the (+/-) cells had the fewest Pt-DNA adducts, and at 24 h mean Pt-DNA adduct levels were 541, 153 and 779 fmol adduct/ mu g DNA for p21 super(WAF1/Cip1) (+/+), (+/-) and (-/-) cells, respectively [P < 0.05 for (+/+) versus (+/-) and (-/-) versus (+/-)]. In order to understand underlying events, we examined p21 super(WAF1/Cip1) messenger RNA (mRNA), cell cycle arrest, and apoptosis in these cells. At 48 h of cisplatin exposure p21 super(WAF1/Cip1) mRNA expression was 2-fold higher in the (+/+) cells, compared to the (+/-) cells. At 24 h, the % of cells in S-phase in cisplatin-exposed cultures, compared to unexposed cultures, was decreased by 51, 40 and 11% in p21 super(WAF1/Cip1) (+/+), (+/-) and (-/-) cells, respectively (P = 0.04, ANOVA). At 24, 48 and 72 h the % of cisplatin-exposed (+/+) cells in apoptosis was 9.4-10.5%, while the cisplatin-exposed (+/-) and (-/-) cells had 1.2-3.7% of cells in apoptosis. The data support the interpretation that DNA replication arrest and apoptosis do not completely explain the low levels of Pt-DNA adducts in the (+/-) cells, and suggest that p21 super(WAF1/Cip1) controls activity resulting in either low Pt-DNA adduct formation or enhanced Pt-DNA adduct removal. JF - Mutagenesis AU - van Gijssel, Hilde E AU - Leil, Tarek A AU - Weinberg, Wendy C AU - Divi, Rao L AU - Olivero, Ofelia A AU - Poirier, Miriam C AD - Laboratory of Cellular Carcinogenesis and Tumor Promotion, CCR, National Cancer Institute, Building 37 Room 4032, NIH Bethesda, MD 20892-4255. Laboratory of ImmunoBiology, Office of Biotechnology Products, CDER, US Food and Drug Administration Bethesda, MD 20892. Valley City State University, 101 College Street S.W. Valley City, ND 58072. Department of Oncology, Mayo Clinic 200 First Street SW., Rochester, MN 55905, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 49 EP - 54 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 22 IS - 1 SN - 0267-8357, 0267-8357 KW - Toxicology Abstracts; Biochemistry Abstracts 2: Nucleic Acids KW - DNA biosynthesis KW - Calcium KW - Apoptosis KW - Skin KW - Replication KW - Adducts KW - Cell cycle KW - Cell culture KW - DNA repair KW - p53 protein KW - Mutagenesis KW - mRNA KW - Gene expression KW - DNA damage KW - Cisplatin KW - cyclin-dependent kinase inhibitor p21 KW - Keratinocytes KW - Drugs KW - X 24310:Pharmaceuticals KW - N 14820:DNA Metabolism & Structure UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19510108?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxicologyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Mutagenesis&rft.atitle=Cisplatin-DNA+damage+in+p21+super%28WAF1%2FCip1%29+deficient+mouse+keratinocytes+exposed+to+cisplatin&rft.au=van+Gijssel%2C+Hilde+E%3BLeil%2C+Tarek+A%3BWeinberg%2C+Wendy+C%3BDivi%2C+Rao+L%3BOlivero%2C+Ofelia+A%3BPoirier%2C+Miriam+C&rft.aulast=van+Gijssel&rft.aufirst=Hilde&rft.date=2007-01-01&rft.volume=22&rft.issue=1&rft.spage=49&rft.isbn=&rft.btitle=&rft.title=Mutagenesis&rft.issn=02678357&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - DNA biosynthesis; Skin; Apoptosis; Calcium; Replication; Adducts; Cell cycle; Cell culture; DNA repair; mRNA; Mutagenesis; p53 protein; Gene expression; DNA damage; cyclin-dependent kinase inhibitor p21; Cisplatin; Keratinocytes; Drugs ER - TY - JOUR T1 - Personal Hair Dye Use and Risks of Glioma, Meningioma, and Acoustic Neuroma among Adults AN - 19508288; 7205578 AB - Previous studies have suggested an association of personal hair dye use with bladder and hematopoietic cancers. Risks for brain tumors are not well understood. The authors investigated associations between use of synthetic hair dyes and risk of brain tumors in a hospital-based case-control study. The study included adults newly diagnosed with glioma (n = 489), meningioma (n = 197), or acoustic neuroma (n = 96) between 1994 and 1998 at three urban US hospitals and 799 controls. Odds ratios were estimated and 95% confidence intervals were calculated using unconditional logistic regression. Detailed exposure histories were obtained by interview. There was no consistent pattern of elevated odds ratios for glioma, meningioma, or acoustic neuroma with use or prolonged use of permanent, semipermanent, temporary, or gradual hair dyes. Although use of permanent brown hair dye for 20 or more years was associated with glioma among women, the estimate was imprecise (odds ratio = 3.8, 95% confidence interval: 1.2, 12.5) and was based on just 13 exposed cases; thus, this could be a chance finding. Overall, there was little consistent evidence for an association of synthetic hair dye use with glioma, meningioma, or acoustic neuroma. However, prolonged use of dark-colored permanent dyes warrants further investigation given the high prevalence of hair dyeing. JF - American Journal of Epidemiology AU - Bluhm, Elizabeth C AU - Zahm, Shelia Hoar AU - Fine, Howard A AU - Black, Peter M AU - Loeffler, Jay S AU - Shapiro, William R AU - Selker, Robert G AU - Inskip, Peter D AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD Y1 - 2007/01/01/ PY - 2007 DA - 2007 Jan 01 SP - 63 EP - 71 PB - Oxford University Press, Oxford Journals Health, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 165 IS - 1 SN - 0002-9262, 0002-9262 KW - CSA Neurosciences Abstracts; Health & Safety Science Abstracts; Risk Abstracts; Toxicology Abstracts KW - Historical account KW - Consumer products KW - Urinary bladder KW - Hair KW - Cancer KW - Neoplasia KW - Brain tumors KW - Dyes KW - Hemopoiesis KW - Hair dyes KW - Glioma KW - brain tumors KW - meningioma KW - Hospitals KW - X 24340:Cosmetics, Toiletries & Household Products KW - H 11000:Diseases/Injuries/Trauma KW - N3 11028:Neuropharmacology & toxicology KW - R2 23060:Medical and environmental health UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19508288?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ariskabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=American+Journal+of+Epidemiology&rft.atitle=Personal+Hair+Dye+Use+and+Risks+of+Glioma%2C+Meningioma%2C+and+Acoustic+Neuroma+among+Adults&rft.au=Bluhm%2C+Elizabeth+C%3BZahm%2C+Shelia+Hoar%3BFine%2C+Howard+A%3BBlack%2C+Peter+M%3BLoeffler%2C+Jay+S%3BShapiro%2C+William+R%3BSelker%2C+Robert+G%3BInskip%2C+Peter+D&rft.aulast=Bluhm&rft.aufirst=Elizabeth&rft.date=2007-01-01&rft.volume=165&rft.issue=1&rft.spage=63&rft.isbn=&rft.btitle=&rft.title=American+Journal+of+Epidemiology&rft.issn=00029262&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Brain tumors; Dyes; Urinary bladder; Hemopoiesis; Hair dyes; Glioma; Hair; Neoplasia; meningioma; Hospitals; Historical account; Consumer products; brain tumors; Cancer ER - TY - JOUR T1 - In vitro induction and selection of fluoroquinolone-resistant mutants of Streptococcus pyogenes strains with multiple emm types AN - 19506859; 7207694 AB - OBJECTIVES: To perform a systematic analysis of point mutations in the quinolone resistance determining regions (QRDRs) of the DNA gyrase and topoisomerase genes of emm type 6 and other emm types of Streptococcus pyogenes strains after in vitro exposure to stepwise increasing concentrations of levofloxacin. METHODS: Twelve parent strains of S. pyogenes, each with a different emm type, were chosen for stepwise exposure to increasing levels of levofloxacin followed by selection of resistant mutants. The QRDRs of gyrA, gyrB, parC and parE correlating to mutants with increased MICs were analysed for point mutations. RESULTS: Multiple mutants with significantly increased MICs were generated from each strain. The amino acid substitutions identified were consistent regardless of emm type and were similar to the mechanisms of resistance reported in clinical isolates of S. pyogenes. The number of induction/selection cycles required for the emergence of key point mutations in gyrA and parC was variable among strains. For each parent-mutant set, when MIC increased, serine-81 of gyrA and serine-79 of parC were the primary targets for amino acid substitutions. No point mutations were found in the QRDRs of gyrB and parE in any of the resistant mutants sequenced. CONCLUSIONS: Despite its intrinsic polymorphism in the QRDR of parC, emm type 6 is not more likely to develop high-level resistance to fluoroquinolones when compared with other emm types. All emm types seem equally inducible to high-level fluoroquinolone resistance. JF - Journal of Antimicrobial Chemotherapy AU - Billal, Dewan S AU - Fedorko, Daniel P AU - Yan, SSteve AU - Hotomi, Muneki AU - Fujihara, Keiji AU - Nelson, Nancy AU - Yamanaka, Noboru AD - Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University 811-1 Kimiidera, Wakayama, 641-8510, Japan. Clinical Center, National Institutes of Health, Department of Health and Human Services Bethesda, MD 20892, USA. Food and Drug Administration, Department of Health and Human Services Rockville, MD 20855, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 28 EP - 34 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 59 IS - 1 SN - 0305-7453, 0305-7453 KW - Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology KW - Clinical isolates KW - Amino acid substitution KW - Fluoroquinolones KW - Levofloxacin KW - Point mutation KW - Quinolones KW - DNA topoisomerase KW - Resistant mutant KW - Minimum inhibitory concentration KW - Streptococcus pyogenes KW - DNA topoisomerase IV KW - A 01350:Microbial Resistance KW - J 02300:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19506859?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Antimicrobial+Chemotherapy&rft.atitle=In+vitro+induction+and+selection+of+fluoroquinolone-resistant+mutants+of+Streptococcus+pyogenes+strains+with+multiple+emm+types&rft.au=Billal%2C+Dewan+S%3BFedorko%2C+Daniel+P%3BYan%2C+SSteve%3BHotomi%2C+Muneki%3BFujihara%2C+Keiji%3BNelson%2C+Nancy%3BYamanaka%2C+Noboru&rft.aulast=Billal&rft.aufirst=Dewan&rft.date=2007-01-01&rft.volume=59&rft.issue=1&rft.spage=28&rft.isbn=&rft.btitle=&rft.title=Journal+of+Antimicrobial+Chemotherapy&rft.issn=03057453&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Clinical isolates; Amino acid substitution; Fluoroquinolones; Levofloxacin; Quinolones; Point mutation; DNA topoisomerase; Resistant mutant; Minimum inhibitory concentration; DNA topoisomerase IV; Streptococcus pyogenes ER - TY - JOUR T1 - Polymorphisms in Th1-type cell-mediated response genes and risk of gastric cancer AN - 19504316; 7206692 AB - Helicobacter pylori infection, the dominant risk factor for gastric cancers, has been shown to elicit T helper type 1 (Th1) polarized immunological responses. We conducted a population-based study of 305 gastric cancer cases and 427 age- and gender-matched controls in Warsaw, Poland, to evaluate the association with several variants in genes responsible for Th1-cell-mediated response. Genotyping was performed on genomic DNA by TaqMan super(TM) assays to determine TNFA (-308 G>A, -417 G>A, -555 G>A, -1036 C>T, -1042 C>A, -1210 T>C), IL1A (-889 C>T), IFNGR2 (Ex7-128 T>C, Ex2-34 C>G and Ex2-16 A>G) and IL12A (IVS2-798 T>A, IVS2-701 C>A and Ex7+277 G>A) polymorphisms. We used unconditional logistic regression to estimate odds ratios (ORs) and 95% confidence intervals (CIs), adjusting for sex, age, education and smoking status. Out of six single nucleotide polymorphisms (SNPs) tested in TNFA, gastric cancer risk was significantly associated with the TNFA (-308 G>A) polymorphism, with ORs of 1.4 (95% CI: 1.0-2.0) for the G/A and 2.5 (95% CI: 1.3-4.9) for the A/A genotype carriers, when compared with the more frequent genotype (G/G) (P-trend T polymorphism was associated with increased risk, with ORs of 1.5 (95% CI: 1.0-2.3) for T/C and 1.7 (95% CI: 1.1-2.7) for C/C carriers when compared with T/T carriers (P-trend = 0.01). Subjects carrying both IFNGR2 Ex7-128 C/C and TNFA -308 A/A genotypes had the highest risk (OR = 5.5, 95% CI: 1.5-19.4), although the interaction was not statistically significant. IL1A (-889 C>T) and the three examined IL12A variants were unrelated to gastric cancer risk. Our findings suggest that two Th1-related polymorphisms (TNFA -308 A>G and IFNGR2 Ex7-128 C>T) may increase the risk of gastric cancer. JF - Carcinogenesis AU - Hou, L AU - El-Omar, E M AU - Chen, J AU - Grillo, P AU - Rabkin, C S AU - Baccarelli, A AU - Yeager, M AU - Chanock, S J AU - Zatonski, W AU - Sobin, L H AU - Lissowska, J AU - Fraumeni, JFJr AU - Chow, W H AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute 6120 Executive Boulevard, EPS 8123, Bethesda, MD 20852-7242, USA. Department of Medicine and Therapeutics, Institute of Medical Sciences, University of Aberdeen Foresterhill, Aberdeen, AB25 2ZD, UK. Molecular and Genetic Epidemiology Center-Epidemiology Unit, Department of Occupational, Clinical and Preventive Medicine, IRCCS Ospedale Maggiore Policlinico Mangiagalli e Regina Elena, I-20122 Milan, Italy. EPOCA Research Center for Clinical, Occupational and Environmental Epidemiology, Department of Occupational Medicine, University of Milan I-20122 Milan, Italy. Core Genotyping Facility, Advanced Technology Center, National Cancer Institute Gaithersburg, MD 20892, USA. Division of Cancer Epidemiology and Prevention, Cancer Center and M.Sklodowska-Curie Institute of Oncology 02-781 Warsaw, Poland. Department of Hepatic and Gastrointestinal Pathology, Armed Forces Institute of Pathology Washington, DC, 20306, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 118 EP - 123 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 28 IS - 1 SN - 0143-3334, 0143-3334 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Helicobacter pylori KW - Age KW - Helper cells KW - Genotyping KW - Gene polymorphism KW - Interleukin 1 KW - Statistical analysis KW - Population studies KW - Infection KW - Smoking KW - Single-nucleotide polymorphism KW - Risk factors KW - Carcinogenesis KW - Lymphocytes T KW - Tumor necrosis factor- alpha KW - genomics KW - Gastric cancer KW - Sex KW - G 07720:Immunogenetics KW - J 02350:Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19504316?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Carcinogenesis&rft.atitle=Polymorphisms+in+Th1-type+cell-mediated+response+genes+and+risk+of+gastric+cancer&rft.au=Hou%2C+L%3BEl-Omar%2C+E+M%3BChen%2C+J%3BGrillo%2C+P%3BRabkin%2C+C+S%3BBaccarelli%2C+A%3BYeager%2C+M%3BChanock%2C+S+J%3BZatonski%2C+W%3BSobin%2C+L+H%3BLissowska%2C+J%3BFraumeni%2C+JFJr%3BChow%2C+W+H&rft.aulast=Hou&rft.aufirst=L&rft.date=2007-01-01&rft.volume=28&rft.issue=1&rft.spage=118&rft.isbn=&rft.btitle=&rft.title=Carcinogenesis&rft.issn=01433334&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Age; Gene polymorphism; Genotyping; Helper cells; Interleukin 1; Statistical analysis; Population studies; Infection; Smoking; Single-nucleotide polymorphism; Risk factors; Carcinogenesis; Lymphocytes T; genomics; Tumor necrosis factor- alpha; Gastric cancer; Sex; Helicobacter pylori ER - TY - JOUR T1 - Engineering controllable anisotropy in electrospun biodegradable nanofibrous scaffolds for musculoskeletal tissue engineering AN - 19503803; 8792212 AB - Many musculoskeletal tissues exhibit significant anisotropic mechanical properties reflective of a highly oriented underlying extracellular matrix. For tissue engineering, recreating this organization of the native tissue remains a challenge. To address this issue, this study explored the fabrication of biodegradable nanofibrous scaffolds composed of aligned fibers via electrospinning onto a rotating target, and characterized their mechanical anisotropy as a function of the production parameters. The characterization showed that nanofiber organization was dependent on the rotation speed of the target; randomly oriented fibers (33% fiber alignment) were produced on a stationary shaft, whereas highly oriented fibers (94% fiber alignment) were produced when rotation speed was increased to 9.3 m/s. Non-aligned scaffolds had an isotropic tensile modulus of 2.1±0.4 MPa, compared to highly anisotropic scaffolds whose modulus was 11.6±3.1 MPa in the presumed fiber direction, suggesting that fiber alignment has a profound effect on the mechanical properties of scaffolds. Mechanical anisotropy was most pronounced at higher rotation speeds, with a greater than 33-fold enhancement of the Young's modulus in the fiber direction compared to perpendicular to the fiber direction when the rotation speed reached 8 m/s. In cell culture, both the organization of actin filaments of human mesenchymal stem cells and the cellular alignment of meniscal fibroblasts were dictated by the prevailing nanofiber orientation. This study demonstrates that controllable and anisotropic mechanical properties of nanofibrous scaffolds can be achieved by dictating nanofiber organization through intelligent scaffold design. JF - Journal of Biomechanics AU - Li, Wan-Ju AU - Mauck, Robert L AU - Cooper, James A AU - Yuan, Xiaoning AU - Tuan, Rocky S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA, tuanr@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 1686 EP - 1693 PB - Elsevier Science, P.O. Box 800 Kidlington Oxford OX5 1DX UK, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl] VL - 40 IS - 8 SN - 0021-9290, 0021-9290 KW - Biotechnology and Bioengineering Abstracts KW - Tissue engineering KW - Tensile properties KW - Anisotropy KW - Mechanical testing KW - Biodegradable scaffolds KW - Cell culture KW - scaffolds KW - Fibroblasts KW - Fibers KW - Stem cells KW - Extracellular matrix KW - meniscus KW - Actin KW - Mesenchyme KW - Filaments KW - Mechanical properties KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19503803?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Biomechanics&rft.atitle=Engineering+controllable+anisotropy+in+electrospun+biodegradable+nanofibrous+scaffolds+for+musculoskeletal+tissue+engineering&rft.au=Li%2C+Wan-Ju%3BMauck%2C+Robert+L%3BCooper%2C+James+A%3BYuan%2C+Xiaoning%3BTuan%2C+Rocky+S&rft.aulast=Li&rft.aufirst=Wan-Ju&rft.date=2007-01-01&rft.volume=40&rft.issue=8&rft.spage=1686&rft.isbn=&rft.btitle=&rft.title=Journal+of+Biomechanics&rft.issn=00219290&rft_id=info:doi/10.1016%2Fj.jbiomech.2006.09.004 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-12-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Anisotropy; Cell culture; Tissue engineering; scaffolds; Fibroblasts; Fibers; Stem cells; meniscus; Extracellular matrix; Actin; Mesenchyme; Filaments; Mechanical properties DO - http://dx.doi.org/10.1016/j.jbiomech.2006.09.004 ER - TY - JOUR T1 - Stem cells of the melanocyte lineage AN - 19499419; 8718907 AB - Melanocytes are pigment-producing cells responsible for coloration of skin and hair. Studies using mouse models have allowed identification of putative melanocyte stem cells within the hair follicle and understanding of hair graying caused by abnormal melanocyte stem cell maintenance. The malignant transformation of melanocytes results in melanoma, the sixth most common cancer in the United States. Recent studies have offered compelling evidence for the existence of cancer stem cells in numerous tumor types, including melanoma. In this review we provide an overview of some of the current findings on follicular melanocyte stem cells, the genetic pathways involved in their regulation and maintenance, and discuss recent studies that support the existence of cancer stem cells in melanoma. JF - Cancer Biomarkers AU - Pavan, William J AD - Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health Bethesda, MD 20892-4472, USA Y1 - 2007 PY - 2007 DA - 2007 SP - 203 EP - 209 PB - IOS Press, Nieuwe Hemweg 6B VL - 3 IS - 4,5 SN - 1574-0153, 1574-0153 KW - Biotechnology and Bioengineering Abstracts KW - Melanocyte stem cells KW - melanoma KW - cancer stem cells KW - Transformation KW - Skin KW - Follicles KW - Animal models KW - Melanocytes KW - Tumors KW - Hair KW - Cancer KW - Melanoma KW - Stem cells KW - Coloration KW - Reviews KW - W 30925:Genetic Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19499419?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=Cancer+Biomarkers&rft.atitle=Stem+cells+of+the+melanocyte+lineage&rft.au=Pavan%2C+William+J&rft.aulast=Pavan&rft.aufirst=William&rft.date=2007-01-01&rft.volume=3&rft.issue=4%2C5&rft.spage=203&rft.isbn=&rft.btitle=&rft.title=Cancer+Biomarkers&rft.issn=15740153&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2009-01-01 N1 - Last updated - 2015-03-30 N1 - SubjectsTermNotLitGenreText - Transformation; Stem cells; Skin; Coloration; Follicles; Reviews; Animal models; Tumors; Melanocytes; Hair; Cancer; Melanoma ER - TY - JOUR T1 - Genome-wide variation and identification of vaccine targets in the Plasmodium falciparum genome AN - 19492216; 7186088 AB - One goal in sequencing the Plasmodium falciparum genome, the agent of the most lethal form of malaria, is to discover vaccine and drug targets super(1). However, identifying those targets in a genome in which [math]60% of genes have unknown functions is an enormous challenge. Because the majority of known malaria antigens and drug-resistant genes are highly polymorphic and under various selective pressures super(2, ) super(3, ) super(4, ) super(5, ) super(6), genome-wide analysis for signatures of selection may lead to discovery of new vaccine and drug candidates. Here we surveyed 3,539 P. falciparum genes ([math]65% of the predicted genes) for polymorphisms and identified various highly polymorphic loci and genes, some of which encode new antigens that we confirmed using human immune sera. Our collections of genome-wide SNPs ([math]65% nonsynonymous) and polymorphic microsatellites and indels provide a high-resolution map (one marker per [math]4 kb) for mapping parasite traits and studying parasite populations. In addition, we report new antigens, providing urgently needed vaccine candidates for disease control. JF - Nature Genetics AU - Mu, Jianbing AU - Awadalla, Philip AU - Duan, Junhui AU - McGee, Kate M AU - Keebler, Jon AU - Seydel, Karl AU - McVean, Gilean A T AU - Su, Xin-Zhuan AD - Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA., xsu@niaid.nih.gov Y1 - 2007/01// PY - 2007 DA - January 2007 SP - 126 EP - 130 PB - Nature Publishing Group, The Macmillan Building 4 Crinan Street London N1 9XW UK, [mailto:feedback@nature.com] VL - 39 IS - 1 SN - 1061-4036, 1061-4036 KW - ASFA 3: Aquatic Pollution & Environmental Quality; ASFA 1: Biological Sciences & Living Resources; Microbiology Abstracts C: Algology, Mycology & Protozoology; Genetics Abstracts KW - Genomes KW - Parasites KW - Human diseases KW - Gene polymorphism KW - Drug resistance KW - Microsatellites KW - Disease control KW - Drug development KW - Malaria KW - Plasmodium falciparum KW - Biopolymorphism KW - Public health KW - Single-nucleotide polymorphism KW - Vaccines KW - Drugs KW - Gene mapping KW - G 07790:Other Microorganisms KW - Q1 08484:Species interactions: parasites and diseases KW - Q5 08524:Public health, medicines, dangerous organisms KW - K 03310:Genetics & Taxonomy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19492216?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aasfaaquaticpollution&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nature+Genetics&rft.atitle=Genome-wide+variation+and+identification+of+vaccine+targets+in+the+Plasmodium+falciparum+genome&rft.au=Mu%2C+Jianbing%3BAwadalla%2C+Philip%3BDuan%2C+Junhui%3BMcGee%2C+Kate+M%3BKeebler%2C+Jon%3BSeydel%2C+Karl%3BMcVean%2C+Gilean+A+T%3BSu%2C+Xin-Zhuan&rft.aulast=Mu&rft.aufirst=Jianbing&rft.date=2007-01-01&rft.volume=39&rft.issue=1&rft.spage=126&rft.isbn=&rft.btitle=&rft.title=Nature+Genetics&rft.issn=10614036&rft_id=info:doi/10.1038%2Fng1924 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2016-05-27 N1 - SubjectsTermNotLitGenreText - Genomes; Parasites; Human diseases; Disease control; Malaria; Vaccines; Biopolymorphism; Drugs; Public health; Single-nucleotide polymorphism; Drug resistance; Gene polymorphism; Microsatellites; Drug development; Gene mapping; Plasmodium falciparum DO - http://dx.doi.org/10.1038/ng1924 ER - TY - JOUR T1 - A second-generation autologous chondrocyte implantation approach to the treatment of focal articular cartilage defects AN - 19487053; 8522243 AB - Autologous chondrocyte implantation (ACI) is the most widely used cell-based surgical procedure for the repair of articular cartilage defects. Challenges to successful ACI outcomes include limitation in defect size and geometry as well as inefficient cell retention. Second-generation ACI procedures have thus focused on developing three-dimensional constructs using native and synthetic biomaterials. Clinically significant and satisfactory results from applying autologous chondrocytes seeded in fibrin within a biodegradable polymeric material were recently reported. In the future, third-generation cell-based articular cartilage repair should focus on the use of chondroprogenitor cells and biofunctionalized biomaterials for more extensive and permanent repair. JF - Arthritis Research & Therapy AU - Tuan, Rocky S AD - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA, tuanr@mail.nih.gov Y1 - 2007 PY - 2007 DA - 2007 SP - 109 PB - BioMed Central Ltd., Middlesex House VL - 9 IS - 5 SN - 1478-6354, 1478-6354 KW - Biotechnology and Bioengineering Abstracts; Calcium & Calcified Tissue Abstracts KW - fibrin KW - Biomaterials KW - Chondrocytes KW - Cartilage (articular) KW - T 2030:Cartilage and Cartilage Diseases KW - W 30920:Tissue Engineering UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19487053?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Arthritis+Research+%26+Therapy&rft.atitle=A+second-generation+autologous+chondrocyte+implantation+approach+to+the+treatment+of+focal+articular+cartilage+defects&rft.au=Tuan%2C+Rocky+S&rft.aulast=Tuan&rft.aufirst=Rocky&rft.date=2007-01-01&rft.volume=9&rft.issue=5&rft.spage=109&rft.isbn=&rft.btitle=&rft.title=Arthritis+Research+%26+Therapy&rft.issn=14786354&rft_id=info:doi/10.1186%2Far2310 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-11-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - fibrin; Biomaterials; Chondrocytes; Cartilage (articular) DO - http://dx.doi.org/10.1186/ar2310 ER - TY - JOUR T1 - Investigating emotion in moral cognition: a review of evidence from functional neuroimaging and neuropsychology AN - 19485687; 8510339 AB - Introduction Human moral decision-making has long been a topic of philosophical debate, and, more recently, a topic for empirical investigation. Central to this investigation is the extent to which emotional processes underlie our decisions about moral right and wrong. Neuroscience offers a unique perspective on this question by addressing whether brain regions associated with emotional processing are involved in moral cognition.Method We conduct a narrative review of neuroscientific studies focused on the role of emotion in morality. Specifically, we describe evidence implicating the ventromedial prefrontal cortex (VMPC), a brain region known to be important for emotional processing.Results Functional imaging studies demonstrate VMPC activation during tasks probing moral cognition. Studies of clinical populations, including patients with VMPC damage, reveal an association between impairments in emotional processing and impairments in moral judgement and behaviour.Conclusions Considered together, these studies indicate that not only are emotions engaged during moral cognition, but that emotions, particularly those mediated by VMPC, are in fact critical for human morality. JF - British Medical Bulletin AU - Young, Liane AU - Koenigs, Michael AD - Department of Psychology , Harvard University , 33 Kirkland Street, 984 William James Hall, Cambridge, MA 02138 , USA,; koenigsm@ninds.nih.gov] lyoung@fas.harvard.edu Y1 - 2007 PY - 2007 DA - 2007 SP - 69 EP - 79 PB - Oxford University Press, Oxford Journals, Great Clarendon Street VL - 84 IS - 1 SN - 0007-1420, 0007-1420 KW - Biotechnology and Bioengineering Abstracts; CSA Neurosciences Abstracts KW - Neuroscience KW - emotion KW - morality KW - ventromedial prefrontal cortex KW - Emotions KW - Decision making KW - Neuroimaging KW - Ethics KW - Computed tomography KW - Population studies KW - Cognition KW - Cortex (prefrontal) KW - W 30910:Imaging KW - N3 11001:Behavioral and Cognitive Neuroscience UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19485687?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=British+Medical+Bulletin&rft.atitle=Investigating+emotion+in+moral+cognition%3A+a+review+of+evidence+from+functional+neuroimaging+and+neuropsychology&rft.au=Young%2C+Liane%3BKoenigs%2C+Michael&rft.aulast=Young&rft.aufirst=Liane&rft.date=2007-01-01&rft.volume=84&rft.issue=1&rft.spage=69&rft.isbn=&rft.btitle=&rft.title=British+Medical+Bulletin&rft.issn=00071420&rft_id=info:doi/10.1093%2Fbmb%2Fldm031 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-10-01 N1 - Last updated - 2015-03-27 N1 - SubjectsTermNotLitGenreText - Decision making; Emotions; Neuroimaging; Ethics; Computed tomography; Population studies; Cortex (prefrontal); Cognition DO - http://dx.doi.org/10.1093/bmb/ldm031 ER - TY - JOUR T1 - Mapping the MRI voxel volume in which thermal noise matches physiological noise - Implications for fMRI AN - 19476834; 7162232 AB - This work addresses the choice of the imaging voxel volume in blood oxygen level dependent (BOLD) functional magnetic resonance imaging (fMRI). Noise of physiological origin that is present in the voxel time course is a prohibitive factor in the detection of small activation-induced BOLD signal changes. If the physiological noise contribution dominates over the temporal fluctuation contribution in the imaging voxel, further increases in the voxel signal-to- noise ratio (SNR) will have diminished corresponding increases in temporal signal-to-noise (TSNR), resulting in reduced corresponding increases in the ability to detect activation induced signal changes. On the other hand, if the thermal and system noise dominate (suggesting a relatively low SNR) further decreases in SNR can prohibit detection of activation-induced signal changes. Here we have proposed and called the "suggested" voxel volume for fMRI the volume where thermal plus system-related and physiological noise variances are equal. Based on this condition we have created maps of fMRI suggested voxel volume from our experimental data at 3T, since this value will spatially vary depending on the contribution of physiologic noise in each voxel. Based on our fast EPI segmentation technique we have found that for gray matter (GM), white matter (WM), and cerebral spinal fluid (CSF) brain compartments the mean suggested cubical voxel volume is: (1.8 mm) super(3), (2.1 mm) super(3) and (1.4 mm) super(3), respectively. Serendipitously, (1.8 mm) super(3) cubical voxel volume for GM approximately matches the cortical thickness, thus optimizing BOLD contrast by minimizing partial volume averaging. The introduced suggested fMRI voxel volume can be a useful parameter for choice of imaging volume for functional studies. JF - NeuroImage AU - Bodurka, J AU - Ye, F AU - Petridou, N AU - Murphy, K AU - Bandettini, P A AD - Functional MRI Facility, National Institute of Mental Health (NIMH), National Institutes of Health (NIH), 10 Center Drive, Building 10, Room 1D80, Bethesda, MD 20892-1148, USA, bodurkaj@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 542 EP - 549 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 34 IS - 2 SN - 1053-8119, 1053-8119 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Functional magnetic resonance imaging KW - Brain KW - Image processing KW - Substantia alba KW - Maps KW - Cerebrospinal fluid KW - Cortex KW - Segmentation KW - Mapping KW - Substantia grisea KW - W 30910:Imaging KW - N3 11145:Methodology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19476834?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NeuroImage&rft.atitle=Mapping+the+MRI+voxel+volume+in+which+thermal+noise+matches+physiological+noise+-+Implications+for+fMRI&rft.au=Bodurka%2C+J%3BYe%2C+F%3BPetridou%2C+N%3BMurphy%2C+K%3BBandettini%2C+P+A&rft.aulast=Bodurka&rft.aufirst=J&rft.date=2007-01-01&rft.volume=34&rft.issue=2&rft.spage=542&rft.isbn=&rft.btitle=&rft.title=NeuroImage&rft.issn=10538119&rft_id=info:doi/10.1016%2Fj.neuroimage.2006.09.039 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Functional magnetic resonance imaging; Neuroimaging; Cerebrospinal fluid; Maps; Substantia alba; Brain; Substantia grisea; Segmentation; Image processing; Mapping; Cortex DO - http://dx.doi.org/10.1016/j.neuroimage.2006.09.039 ER - TY - JOUR T1 - Neural dynamics for facial threat processing as revealed by gamma band synchronization using MEG AN - 19473799; 7162255 AB - Facial threat conveys important information about imminent environmental danger. The rapid detection of this information is critical for survival and social interaction. However, due to technical and methodological difficulties, the spatiotemporal profile for facial threat processing is unknown. By utilizing magnetoencephalography (MEG), a brain-imaging technique with superb temporal resolution and fairly good spatial resolution, Synthetic Aperture Magnetometry (SAM), a recently developed source analysis technique, and a sliding window analysis, we identified the spatiotemporal development of facial threat processing in the gamma frequency band. We also tested the dual-route hypothesis by LeDoux who proposed, based on animal research, that there are two routes to the amygdala: a quick subcortical route and a slower and cortical route. Direct evidence with humans supporting this model has been lacking. Moreover, it has been unclear whether the subcortical route responds specifically to fearful expressions or to threatening expressions in general. We found early event- related synchronizations (ERS) in response to fearful faces in the hypothalamus/thalamus area (10-20 ms) and then the amygdala (20-30 ms). This was even earlier than the ERS response seen to fearful faces in visual cortex (40-50 ms). These data support LeDouxs suggestion of a quick, subcortical thamalo- amygdala route. Moreover, this route was specific for fear expressions; the ERS response in the amygdala to angry expressions had a late onset (150-160 ms). The ERS onset in prefrontal cortex followed that seen within the amygdala (around 160-210 ms). This is consistent with its role in higher-level emotional/cognitive processing. JF - NeuroImage AU - Luo, Qian AU - Holroyd, Tom AU - Jones, Matthew AU - Hendler, Talma AU - Blair, James AD - Mood and Anxiety Disorders Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-2670, USA, luoj@mail.nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 839 EP - 847 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 34 IS - 2 SN - 1053-8119, 1053-8119 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Magnetoencephalography KW - Emotions KW - Fear KW - Synchronization KW - Survival KW - Thalamus KW - Cortex KW - Information processing KW - Social interactions KW - Cognitive ability KW - Cortex (visual) KW - Amygdala KW - Cortex (prefrontal) KW - W 30910:Imaging KW - N3 11145:Methodology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19473799?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NeuroImage&rft.atitle=Neural+dynamics+for+facial+threat+processing+as+revealed+by+gamma+band+synchronization+using+MEG&rft.au=Luo%2C+Qian%3BHolroyd%2C+Tom%3BJones%2C+Matthew%3BHendler%2C+Talma%3BBlair%2C+James&rft.aulast=Luo&rft.aufirst=Qian&rft.date=2007-01-01&rft.volume=34&rft.issue=2&rft.spage=839&rft.isbn=&rft.btitle=&rft.title=NeuroImage&rft.issn=10538119&rft_id=info:doi/10.1016%2Fj.neuroimage.2006.09.023 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Amygdala; Cortex (prefrontal); Synchronization; Cortex (visual); Emotions; Social interactions; Survival; Information processing; Thalamus; Cognitive ability; Magnetoencephalography; Cortex; Fear DO - http://dx.doi.org/10.1016/j.neuroimage.2006.09.023 ER - TY - JOUR T1 - How long to scan? The relationship between fMRI temporal signal to noise ratio and necessary scan duration AN - 19470958; 7162234 AB - Recent advances in MRI receiver and coil technologies have significantly improved image signal-to-noise ratios (SNR) and thus temporal SNR (TSNR). These gains in SNR and TSNR have allowed the detection of fMRI signal changes at higher spatial resolution and therefore have increased the potential to localize small brain structures such as cortical layers and columns. The majority of current fMRI processing strategies employ multi-subject averaging and therefore require spatial smoothing and normalization, effectively negating these gains in spatial resolution higher than about 10 mm super(3). Reliable detection of activation in single subjects at high resolution is becoming a more common desire among fMRI researchers who are interested in comparing individuals rather than populations. Since TSNR decreases with voxel volume, detection of activation at higher resolutions requires longer scan durations. The relationship between TSNR, voxel volume and detectability is highly non-linear. In this study, the relationship between TSNR and the necessary fMRI scan duration required to obtain significant results at varying P values is determined both experimentally and theoretically. The results demonstrate that, with a TSNR of 50, detection of activation of above 2% requires at most 350 scan volumes (when steps are taken to remove the influence of physiological noise from the data). Importantly, these results also demonstrate that, for activation magnitude on the order of 1%, the scan duration required is more sensitive to the TSNR level than at 2%. This study showed that with voxel volumes of 10 mm super(3) at 3 T, and a corresponding TSNR of 50, the required number of time points that guarantees detection of signal changes of 1% is about 860, but if TSNR increases by only 20%, the time for detection decreases by more than 30%. More than just being an exercise in numbers, these results imply that imaging of columnar resolution (effect size = 1% and assuming a TR of 1 s) at 3 T will require either 10 min for a TSNR of 60 or 40 min for a TSNR of 30. The implication is that at these resolutions, TSNR is likely to be critical for determining success or failure of an experiment. JF - NeuroImage AU - Murphy, Kevin AU - Bodurka, Jerzy AU - Bandettini, Peter A AD - Section on Functional Imaging Methods, National Institute of Mental Health, NIH, Bethesda, MD 20892-1148, USA, bandettini@nih.gov Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - 565 EP - 574 PB - Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, [mailto:usinfo-f@elsevier.com], [URL:http://www.elsevier.nl] VL - 34 IS - 2 SN - 1053-8119, 1053-8119 KW - CSA Neurosciences Abstracts; Biotechnology and Bioengineering Abstracts KW - Neuroimaging KW - Cortex KW - Functional magnetic resonance imaging KW - Brain KW - Physical training KW - W 30910:Imaging KW - N3 11145:Methodology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19470958?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=NeuroImage&rft.atitle=How+long+to+scan%3F+The+relationship+between+fMRI+temporal+signal+to+noise+ratio+and+necessary+scan+duration&rft.au=Murphy%2C+Kevin%3BBodurka%2C+Jerzy%3BBandettini%2C+Peter+A&rft.aulast=Murphy&rft.aufirst=Kevin&rft.date=2007-01-01&rft.volume=34&rft.issue=2&rft.spage=565&rft.isbn=&rft.btitle=&rft.title=NeuroImage&rft.issn=10538119&rft_id=info:doi/10.1016%2Fj.neuroimage.2006.09.032 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Functional magnetic resonance imaging; Neuroimaging; Cortex; Brain; Physical training DO - http://dx.doi.org/10.1016/j.neuroimage.2006.09.032 ER - TY - JOUR T1 - MMDB: annotating protein sequences with Entrez's 3D-structure database AN - 19463495; 7254418 AB - Three-dimensional (3D) structure is now known for a large fraction of all protein families. Thus, it has become rather likely that one will find a homolog with known 3D structure when searching a sequence database with an arbitrary query sequence. Depending on the extent of similarity, such neighbor relationships may allow one to infer biological function and to identify functional sites such as binding motifs or catalytic centers. Entrez's 3D-structure database, the Molecular Modeling Database (MMDB), provides easy access to the richness of 3D structure data and its large potential for functional annotation. Entrez's search engine offers several tools to assist biologist users: (i) links between databases, such as between protein sequences and structures, (ii) pre-computed sequence and structure neighbors, (iii) visualization of structure and sequence/structure alignment. Here, we describe an annotation service that combines some of these tools automatically, Entrez's 'Related Structure' links. For all proteins in Entrez, similar sequences with known 3D structure are detected by BLAST and alignments are recorded. The 'Related Structure' service summarizes this information and presents 3D views mapping sequence residues onto all 3D structures available in MMDB (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=structure). JF - Nucleic Acids Research AU - Wang, Yanli AU - Addess, Kenneth J AU - Chen, Jie AU - Geer, Lewis Y AU - He, Jane AU - He, Siqian AU - Lu, Shennan AU - Madej, Thomas AU - Marchler-Bauer, Aron AU - Thiessen, Paul A AU - Zhang, Naigong AU - Bryant, Stephen H AD - National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Bethesda, MD 20894, USA Y1 - 2007/01// PY - 2007 DA - Jan 2007 SP - D298 EP - D300 PB - Oxford University Press, Oxford Journals, Great Clarendon Street Oxford OX2 6DP UK, [mailto:jnl.samples@oup.co.uk], [URL:http://www3.oup.co.uk/jnls/] VL - 35 SN - 0305-1048, 0305-1048 KW - Biochemistry Abstracts 2: Nucleic Acids; Biotechnology and Bioengineering Abstracts KW - Molecular modelling KW - Databases KW - protein families KW - N 14845:Miscellaneous KW - W 30960:Bioinformatics & Computer Applications UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19463495?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Nucleic+Acids+Research&rft.atitle=MMDB%3A+annotating+protein+sequences+with+Entrez%27s+3D-structure+database&rft.au=Wang%2C+Yanli%3BAddess%2C+Kenneth+J%3BChen%2C+Jie%3BGeer%2C+Lewis+Y%3BHe%2C+Jane%3BHe%2C+Siqian%3BLu%2C+Shennan%3BMadej%2C+Thomas%3BMarchler-Bauer%2C+Aron%3BThiessen%2C+Paul+A%3BZhang%2C+Naigong%3BBryant%2C+Stephen+H&rft.aulast=Wang&rft.aufirst=Yanli&rft.date=2007-01-01&rft.volume=35&rft.issue=&rft.spage=D298&rft.isbn=&rft.btitle=&rft.title=Nucleic+Acids+Research&rft.issn=03051048&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2011-12-14 N1 - SubjectsTermNotLitGenreText - Databases; Molecular modelling; protein families ER - TY - JOUR T1 - Assessing the efficacy, effectiveness, and cost-effectiveness of assistive technology interventions for enhancing mobility AN - 1347817020; 201306144 AB - Purpose. The aim of this paper is to highlight the contributions that complementary efficacy, effectiveness, and cost-effectiveness studies can make to assessing the outcomes of assistive technology interventions for enhancing mobility. Method. The terms, 'assistive technology outcomes research' and 'assistive technology interventions', are defined. Several bases are examined for the shortage of outcomes research pertaining to mobility-related assistive technology interventions. Three presuppositions are described for the research strategy of interlocking studies being recommended. They are assigning priority to evaluating both recently developed assistive technologies and ones that have long been available, acknowledging the complexity of assistive technology as an intervention, and appreciating the trade-offs necessary for strengthening studies' internal and external validity. Some key study preparations are considered, including treatment theory, treatment specification, and the selection of outcome domains and measures. The essential features of efficacy, effectiveness, and cost-effectiveness studies are outlined, and their interdependence is stressed. Results and Conclusions. To assess the outcomes of assistive technology interventions for mobility in ways that are both methodologically sound and relevant to stakeholder needs, a research strategy is required involving mutually reinforcing efficacy, effectiveness, and cost-effectiveness studies. Collaborative arrangements and funding methods are discussed for fostering the needed research. Adapted from the source document. JF - Disability and Rehabilitation: Assistive Technology AU - Fuhrer, Marcus J AD - National Center for Medical Rehabilitation Research, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA fuhrerm@mail.nih.gov Y1 - 2007///0, PY - 2007 DA - 0, 2007 SP - 149 EP - 158 PB - Informa HealthCare, Abingdon UK VL - 2 IS - 3 SN - 1748-3107, 1748-3107 KW - Assistive technology, mobility, outcomes research, efficacy, effectiveness, cost-effectiveness KW - Mobility KW - Efficacy KW - Interventions KW - Specification KW - Cost effectiveness KW - Technical aids KW - article UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1347817020?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Aassia&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Disability+and+Rehabilitation%3A+Assistive+Technology&rft.atitle=Assessing+the+efficacy%2C+effectiveness%2C+and+cost-effectiveness+of+assistive+technology+interventions+for+enhancing+mobility&rft.au=Fuhrer%2C+Marcus+J&rft.aulast=Fuhrer&rft.aufirst=Marcus&rft.date=2007-01-01&rft.volume=2&rft.issue=3&rft.spage=149&rft.isbn=&rft.btitle=&rft.title=Disability+and+Rehabilitation%3A+Assistive+Technology&rft.issn=17483107&rft_id=info:doi/10.1080%2F17483100701374355 LA - English DB - Applied Social Sciences Index & Abstracts (ASSIA) N1 - Date revised - 2013-05-01 N1 - Last updated - 2016-09-27 N1 - SubjectsTermNotLitGenreText - Technical aids; Interventions; Cost effectiveness; Efficacy; Mobility; Specification DO - http://dx.doi.org/10.1080/17483100701374355 ER - TY - JOUR T1 - New psittacosaurid highlights skull enlargement in horned dinosaurs AN - 1244669552; 2013-006285 AB - Anew psittacosaurid is based on a nearly complete articulated skeleton from northeastern China that differs principally in skull size as compared to the most common and widespread species, Psittacosaurus mongoliensis. The skull of Psittacosaurus major sp. nov., is 25% larger despite very similar postcranial skeletal dimensions. Such selective skull enlargement is very unusual. Skull size in ceratopsians, in general, scales with positive allometry relative to body mass: species of greater mass have proportionately larger skulls. This pattern stands in marked contrast to that for other vertebrate herbivores, in which larger-bodied species either have proportionately similar or smaller skulls relative to body mass. Larger-bodied ceratopsians evolved skulls that are 50% or more of trunk length-as measured without their expansive cranial frill. Although contemporaneous duck-billed dinosaurs also exhibit some positive allometry in the skull, skull length remains approximately 35% of trunk length. The evolution of extraordinary absolute and relative skull size among ceratopsians appears to have been driven by sexual selection and involved the tandem evolution of reduced head mobility and an obligate quadrupedal posture. JF - Acta Palaeontologica Polonica AU - Sereno, Paul C AU - Zhao, Xijin AU - Brown, Lorin AU - Tan, Lin Y1 - 2007 PY - 2007 DA - 2007 SP - 275 EP - 284 PB - Panstwowe Wydawnictwo Naukowe, Warsaw VL - 52 IS - 2 SN - 0567-7920, 0567-7920 KW - Diapsida KW - Far East KW - Psittacosaurus major KW - Cretaceous KW - northeastern China KW - Liaoning China KW - new taxa KW - Psittacosauridae KW - Archosauria KW - skull KW - Ceratopsia KW - upper Mesozoic KW - Psittacosaurus KW - dinosaurs KW - taxonomy KW - Asia KW - China KW - Chordata KW - Beipiao China KW - Mesozoic KW - Reptilia KW - Yixian Formation KW - Marginocephalia KW - Vertebrata KW - Ornithischia KW - Tetrapoda KW - 11:Vertebrate paleontology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/1244669552?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ageorefmodule&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Acta+Palaeontologica+Polonica&rft.atitle=New+psittacosaurid+highlights+skull+enlargement+in+horned+dinosaurs&rft.au=Sereno%2C+Paul+C%3BZhao%2C+Xijin%3BBrown%2C+Lorin%3BTan%2C+Lin&rft.aulast=Sereno&rft.aufirst=Paul&rft.date=2007-01-01&rft.volume=52&rft.issue=2&rft.spage=275&rft.isbn=&rft.btitle=&rft.title=Acta+Palaeontologica+Polonica&rft.issn=05677920&rft_id=info:doi/ L2 - http://www.app.pan.pl/archive/published/app52/app52-275.pdf http://app.pan.pl/ LA - English DB - GeoRef N1 - Copyright - GeoRef, Copyright 2013, American Geosciences Institute. Reference includes data supplied by Panstwowy Instytut Geologiczny, Warsaw, Poland N1 - Date revised - 2013-01-01 N1 - Number of references - 52 N1 - Document feature - illus. incl. 3 tables N1 - SuppNotes - Accessed on Dec. 14, 2012 N1 - Last updated - 2012-12-27 N1 - CODEN - APGPAC N1 - SubjectsTermNotLitGenreText - Archosauria; Asia; Beipiao China; Ceratopsia; China; Chordata; Cretaceous; Diapsida; dinosaurs; Far East; Liaoning China; Marginocephalia; Mesozoic; new taxa; northeastern China; Ornithischia; Psittacosauridae; Psittacosaurus; Psittacosaurus major; Reptilia; skull; taxonomy; Tetrapoda; upper Mesozoic; Vertebrata; Yixian Formation ER - TY - JOUR T1 - Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937. AN - 68295031; 17192190 AB - Breast cancer is the second leading cause of cancer deaths in women in the United States. Although the causes of this disease are incompletely understood, oxidative DNA damage is presumed to play a critical role in breast carcinogenesis. A common oxidatively induced DNA lesion is 8-hydroxyguanine (8-OH-Gua), which has been implicated in carcinogenesis. The aim of this study was to investigate the ability of HCC1937 and MCF-7 breast cancer cell lines to repair 8-OH-Gua relative to a nonmalignant human mammary epithelial cell line, AG11134. We used oligonucleotide incision assay to analyze the ability of the two breast cancer cell lines to incise 8-OH-Gua relative to the control cell line. Liquid chromatography/mass spectrometry (LC/MS) was used to measure the levels of 8-OH-Gua as its nucleoside, 8-OH-dG in the cell lines after exposure to H2O2 followed by 30 min repair period. Protein expression levels were determined by Western blot analysis, while the hOGG1 mRNA levels were analyzed by RT-PCR. Complementation of hOGG1 activity in HCC1937 cells was assessed by addition of the purified protein in the incision assay, and in vivo by transfection of pFlagCMV-4-hOGG1. Clonogenic survival assay was used to determine sensitivity after H2O2-mediated oxidative stress. We show that the HCC1937 breast cancer cells have diminished ability to incise 8-OH-Gua and they accumulate higher levels of 8-OH-dG in the nuclear genome after H2O2 treatment despite a 30 min repair period when compared to the nonmalignant mammary cells. The defective incision of 8-OH-Gua was consistent with expression of undetectable amounts of hOGG1 in HCC1937 cells. The reduced incision activity was significantly stimulated by addition of purified hOGG1. Furthermore, transfection of pFlagCMV-4-hOGG1 in HCC1937 cells resulted in enhanced incision of 8-OH-Gua. HCC1937 cells are more sensitive to high levels of H2O2 and have up-regulated SOD1 and SOD2. This study provides evidence for inefficient repair of 8-OH-Gua in HCC1937 breast cancer cell line and directly implicates hOGG1 in this defect. JF - BMC cancer AU - Nyaga, Simon G AU - Lohani, Althaf AU - Jaruga, Pawel AU - Trzeciak, Andrzej R AU - Dizdaroglu, Miral AU - Evans, Michele K AD - Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. nyagas@grc.nia.nih.gov Y1 - 2006/12/27/ PY - 2006 DA - 2006 Dec 27 SP - 297 VL - 6 KW - Cell Extracts KW - 0 KW - DNA Primers KW - Oligodeoxyribonucleotides KW - RNA, Neoplasm KW - 8-hydroxyguanine KW - 5614-64-2 KW - Guanine KW - 5Z93L87A1R KW - Hydrogen Peroxide KW - BBX060AN9V KW - Index Medicus KW - Mitochondria -- physiology KW - DNA Repair KW - Carcinoma, Ductal -- genetics KW - Cell Nucleus -- ultrastructure KW - Humans KW - Hydrogen Peroxide -- pharmacology KW - Cell Line, Tumor KW - RNA, Neoplasm -- genetics KW - Reverse Transcriptase Polymerase Chain Reaction KW - Cell Extracts -- isolation & purification KW - Base Sequence KW - RNA, Neoplasm -- isolation & purification KW - Mitochondria -- ultrastructure KW - Transfection KW - Cell Nucleus -- physiology KW - Female KW - Breast Neoplasms -- genetics KW - Guanine -- analogs & derivatives KW - Guanine -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68295031?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=BMC+cancer&rft.atitle=Reduced+repair+of+8-hydroxyguanine+in+the+human+breast+cancer+cell+line%2C+HCC1937.&rft.au=Nyaga%2C+Simon+G%3BLohani%2C+Althaf%3BJaruga%2C+Pawel%3BTrzeciak%2C+Andrzej+R%3BDizdaroglu%2C+Miral%3BEvans%2C+Michele+K&rft.aulast=Nyaga&rft.aufirst=Simon&rft.date=2006-12-27&rft.volume=6&rft.issue=&rft.spage=297&rft.isbn=&rft.btitle=&rft.title=BMC+cancer&rft.issn=1471-2407&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-02 N1 - Date created - 2007-01-17 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: FASEB J. 2003 Apr;17(6):668-74 [12665480] J Bacteriol. 1992 Oct;174(20):6321-5 [1328155] Toxicol Sci. 2003 Sep;75(1):74-81 [12805649] Mutat Res. 2003 Oct 29;531(1-2):231-51 [14637258] J Biol Chem. 2003 Dec 26;278(52):52914-8 [14578343] Nucleic Acids Res. 2004;32(11):e87 [15215337] Carcinogenesis. 2004 Aug;25(8):1359-70 [15044326] Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13300-5 [10557315] Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4156-61 [10725358] Nucleic Acids Res. 2000 Jul 15;28(14):2672-8 [10908322] Nucleic Acids Res. 2001 Jan 15;29(2):430-8 [11139613] Nucleic Acids Res. 2001 Mar 15;29(6):1285-92 [11238994] Mutat Res. 2001 Jun 5;486(1):31-40 [11356334] Carcinogenesis. 2001 Sep;22(9):1459-63 [11532868] Mol Carcinog. 2001 Aug;31(4):214-23 [11536371] Prog Nucleic Acid Res Mol Biol. 2001;68:193-205 [11554297] Cancer. 1993 May 15;71(10):3036-43 [8387875] Trends Genet. 1993 Jul;9(7):246-9 [8379000] Mutat Res. 1995 May;336(3):257-67 [7739614] Nat Genet. 1996 Jul;13(3):266-8 [8673121] Br J Cancer. 1996 Jul;74(1):1-5 [8679441] Curr Biol. 1996 Aug 1;6(8):968-80 [8805338] FEBS Lett. 1997 Jan 2;400(1):25-30 [9000507] FASEB J. 1997 Jan;11(1):68-76 [9034168] Mol Gen Genet. 1997 Mar 26;254(2):171-8 [9108279] Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7429-34 [9207108] Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8010-5 [9223305] Cancer Res. 1998 Aug 1;58(15):3237-42 [9699648] J Biol Chem. 1998 Dec 11;273(50):33811-6 [9837971] Oncogene. 1998 Dec 17;17(24):3115-24 [9872327] Nucleic Acids Res. 1999 Mar 1;27(5):1365-8 [9973627] J Biol Chem. 1999 Jun 25;274(26):18808-12 [10373498] Nucleic Acids Res. 1999 Oct 15;27(20):4001-7 [10497264] Biochemistry. 2004 Dec 21;43(50):15909-14 [15595846] Oncogene. 2005 Jun 30;24(28):4496-508 [15856018] FASEB J. 2006 Jan;20(1):112-4 [16293709] Nucleic Acids Res. 2006;34(5):1620-32 [16549874] Nucleic Acids Res. 2002 Feb 1;30(3):782-93 [11809892] Cancer Res. 2002 Mar 1;62(5):1349-55 [11888904] Methods Mol Biol. 2002;197:227-44 [12013799] Am J Physiol Lung Cell Mol Physiol. 2002 Jul;283(1):L205-10 [12060578] J Biol Chem. 2002 Nov 22;277(47):44932-7 [12244119] Oncogene. 2002 Dec 16;21(58):8935-48 [12483510] Cancer Res. 2002 Dec 15;62(24):7230-3 [12499263] Cancer Res. 2004 Sep 1;64(17):6233-9 [15342409] Biochemistry. 2004 Sep 14;43(36):11596-604 [15350146] Nucleic Acids Res. 1988 Feb 11;16(3):1215 [3344216] Proc Natl Acad Sci U S A. 1988 Sep;85(17):6465-7 [3413108] Arch Biochem Biophys. 1991 Mar;285(2):317-24 [1654771] Cancer Res. 1991 Oct 1;51(19):5430-2 [1655250] FEBS Lett. 1992 Sep 7;309(2):193-8 [1324197] FASEB J. 2003 Jul;17(10):1195-214 [12832285] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Transcriptional changes common to human cocaine, cannabis and phencyclidine abuse. AN - 68278645; 17205118 AB - A major goal of drug abuse research is to identify and understand drug-induced changes in brain function that are common to many or all drugs of abuse. As these may underlie drug dependence and addiction, the purpose of the present study was to examine if different drugs of abuse effect changes in gene expression that converge in common molecular pathways. Microarray analysis was employed to assay brain gene expression in postmortem anterior prefrontal cortex (aPFC) from 42 human cocaine, cannabis and/or phencyclidine abuse cases and 30 control cases, which were characterized by toxicology and drug abuse history. Common transcriptional changes were demonstrated for a majority of drug abuse cases (N = 34), representing a number of consistently changed functional classes: Calmodulin-related transcripts (CALM1, CALM2, CAMK2B) were decreased, while transcripts related to cholesterol biosynthesis and trafficking (FDFT1, APOL2, SCARB1), and Golgi/endoplasmic reticulum (ER) functions (SEMA3B, GCC1) were all increased. Quantitative PCR validated decreases in calmodulin 2 (CALM2) mRNA and increases in apolipoprotein L, 2 (APOL2) and semaphorin 3B (SEMA3B) mRNA for individual cases. A comparison between control cases with and without cardiovascular disease and elevated body mass index indicated that these changes were not due to general cellular and metabolic stress, but appeared specific to the use of drugs. Therefore, humans who abused cocaine, cannabis and/or phencyclidine share a decrease in transcription of calmodulin-related genes and increased transcription related to lipid/cholesterol and Golgi/ER function. These changes represent common molecular features of drug abuse, which may underlie changes in synaptic function and plasticity that could have important ramifications for decision-making capabilities in drug abusers. JF - PloS one AU - Lehrmann, Elin AU - Colantuoni, Carlo AU - Deep-Soboslay, Amy AU - Becker, Kevin G AU - Lowe, Ross AU - Huestis, Marilyn A AU - Hyde, Thomas M AU - Kleinman, Joel E AU - Freed, William J AD - Cellular Neurobiology Research Branch and Chemistry and Drug Metabolism Section, National Institute on Drug Abuse (NIDA) Intramural Research Program, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland, United States of America. elehrman@intra.nida.nih.gov Y1 - 2006/12/27/ PY - 2006 DA - 2006 Dec 27 SP - 1 VL - 1 KW - Calmodulin KW - 0 KW - RNA, Messenger KW - Cholesterol KW - 97C5T2UQ7J KW - Index Medicus KW - Young Adult KW - Autopsy KW - Prefrontal Cortex -- metabolism KW - Oligonucleotide Array Sequence Analysis KW - Humans KW - Lipid Metabolism -- genetics KW - RNA, Messenger -- genetics KW - Polymerase Chain Reaction KW - RNA, Messenger -- metabolism KW - Cholesterol -- metabolism KW - Golgi Apparatus -- genetics KW - Adult KW - Case-Control Studies KW - Endoplasmic Reticulum -- genetics KW - Calmodulin -- genetics KW - Female KW - Male KW - Phencyclidine Abuse -- metabolism KW - Phencyclidine Abuse -- genetics KW - Cocaine-Related Disorders -- genetics KW - Marijuana Abuse -- metabolism KW - Marijuana Abuse -- genetics KW - Transcription, Genetic KW - Cocaine-Related Disorders -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68278645?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=PloS+one&rft.atitle=Transcriptional+changes+common+to+human+cocaine%2C+cannabis+and+phencyclidine+abuse.&rft.au=Lehrmann%2C+Elin%3BColantuoni%2C+Carlo%3BDeep-Soboslay%2C+Amy%3BBecker%2C+Kevin+G%3BLowe%2C+Ross%3BHuestis%2C+Marilyn+A%3BHyde%2C+Thomas+M%3BKleinman%2C+Joel+E%3BFreed%2C+William+J&rft.aulast=Lehrmann&rft.aufirst=Elin&rft.date=2006-12-27&rft.volume=1&rft.issue=&rft.spage=e114&rft.isbn=&rft.btitle=&rft.title=PloS+one&rft.issn=1932-6203&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2010-03-16 N1 - Date created - 2007-01-05 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Nature. 2000 Apr 6;404(6778):567-73 [10766232] Nat Rev Neurosci. 2005 Apr;6(4):267-76 [15803158] Neuron. 2000 Oct;28(1):53-67 [11086983] Synapse. 2001 Mar 1;39(3):257-66 [11169774] Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4746-51 [11296301] J Neural Transm (Vienna). 2001;108(3):335-47 [11341485] Cereb Cortex. 2001 Jun;11(6):558-71 [11375917] Bioinformatics. 2002 Jan;18(1):207-8 [11836235] Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4680-5 [11930015] Genomics. 2002 Apr;79(4):539-46 [11944986] Biol Psychiatry. 2002 Apr 15;51(8):605-11 [11955460] J Neurochem. 2002 May;81(4):802-13 [12065639] Am J Psychiatry. 2002 Oct;159(10):1642-52 [12359667] Schizophr Res. 2002 Nov 1;58(1):11-20 [12363385] Synapse. 2002 Dec 15;46(4):271-9 [12373743] Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903 [12477932] Pharmacogenomics J. 2003;3(1):27-40 [12629581] Biochim Biophys Acta. 2003 Mar 10;1610(2):271-80 [12648780] J Mol Diagn. 2003 May;5(2):73-81 [12707371] J Neurochem. 2003 May;85(4):911-24 [12716423] Drug Alcohol Depend. 2003 May 21;70(2):117-25 [12732403] Addict Biol. 2003 Mar;8(1):67-74 [12745418] J Neurosci Res. 2003 Jun 15;72(6):756-67 [12774316] J Neurosci. 2003 Jul 16;23(15):6188-99 [12867502] Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10523-8 [12939407] Nat Rev Neurosci. 2004 Mar;5(3):184-94 [14976518] J Neurochem. 2004 Mar;88(5):1211-9 [15009677] J Neurochem. 2004 Jul;90(1):220-30 [15198681] Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15506-11 [15483108] Alcohol Clin Exp Res. 1998 May;22(3 Suppl):88S-92S [9622380] Neuron. 1998 Sep;21(3):593-606 [9768845] Biochim Biophys Acta. 1999 May 31;1454(1):11-8 [10354510] Mol Cell Neurosci. 2005 Feb;28(2):275-91 [15691709] Cereb Cortex. 2005 Mar;15(3):341-8 [15269111] J Neurosci. 2005 Apr 6;25(14):3613-20 [15814792] Neuron. 2005 Oct 6;48(1):63-75 [16202709] Nat Neurosci. 2005 Nov;8(11):1445-9 [16251986] Trends Pharmacol Sci. 2005 Dec;26(12):645-53 [16253351] Neuroimage. 2005 Dec;28(4):904-14 [16061398] Neuron. 2005 Dec 8;48(5):757-71 [16337914] J Mass Spectrom. 2006 Feb;41(2):175-84 [16382483] Int J Neuropsychopharmacol. 2006 Apr;9(2):147-53 [16004619] J Neurosci. 2006 Mar 15;26(11):2971-80 [16540575] Biol Psychiatry. 2006 Sep 15;60(6):650-8 [16997002] Nat Neurosci. 2005 Apr;8(4):468-75 [15793579] Synapse. 2000 Jun 1;36(3):155-66 [10819896] N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Mutagenesis and modeling of the peroxiredoxin (Prx) complex with the NMR structure of ATP-bound human sulfiredoxin implicate aspartate 187 of Prx I as the catalytic residue in ATP hydrolysis. AN - 68252271; 17176052 AB - The catalytic cysteine of certain members of the peroxiredoxin (Prx) family can be hyperoxidized to cysteinesulfinic acid during reduction of peroxides. Sulfiredoxin is responsible for the ATP-dependent reduction of cysteinesulfinic acid (SO2H) of hyperoxidized Prx. Here we report the NMR solution structure of human sulfiredoxin (hSrx), both with and without bound ATP, and we model the complex of ATP-bound hSrx with Prx. Binding ATP causes only small changes in the NMR structure of hSrx, and the bound ATP conformation is quite similar to that seen for the previously reported X-ray structure of the ADP-hSrx complex. Although hSrx binds ATP, it does not catalyze hydrolysis by itself and has no catalytic acid residue typical of most ATPase and kinase family proteins. For modeling the complex, the ATP-bound hSrx was docked to hyperoxidized Prx II using EMAP of CHARMM. In the model complex, Asn186 of Prx II (Asp187 of Prx I) is in contact with the hSrx-bound ATP beta- and gamma-phosphate groups. Asp187 of Prx I was mutated to alanine and asparagine, and binding and activity of the mutants with hSrx were compared to those of the wild type. For the D187N mutant, both binding and hydrolysis and reduction activities were comparable to those of the wild type, whereas for D187A, binding was unimpaired but ATP hydrolysis and reduction did not occur. The modeling and mutagenesis analyses strongly implicate Asp187 of Prx I as the catalytic residue responsible for ATP hydrolysis in the cysteinesulfinic acid reduction of Prx by hSrx. JF - Biochemistry AU - Lee, Duck-Yeon AU - Park, Sung Jun AU - Jeong, Woojin AU - Sung, Ho Jin AU - Oho, Taena AU - Wu, Xiongwu AU - Rhee, Sue Goo AU - Gruschus, James M AD - Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-0301, USA. Y1 - 2006/12/26/ PY - 2006 DA - 2006 Dec 26 SP - 15301 EP - 15309 VL - 45 IS - 51 KW - cysteine sulfinic acid KW - 2381-08-0 KW - Aspartic Acid KW - 30KYC7MIAI KW - Asparagine KW - 7006-34-0 KW - Adenosine Triphosphate KW - 8L70Q75FXE KW - Oxidoreductases KW - EC 1.- KW - Peroxiredoxins KW - EC 1.11.1.15 KW - Oxidoreductases Acting on Sulfur Group Donors KW - EC 1.8.- KW - SRXN1 protein, human KW - EC 1.8.98.2 KW - Cysteine KW - K848JZ4886 KW - Index Medicus KW - Oxidation-Reduction KW - Mutagenesis, Site-Directed KW - Cysteine -- chemistry KW - Humans KW - Asparagine -- genetics KW - Crystallography, X-Ray KW - Protein Structure, Tertiary KW - Hydrolysis KW - Cysteine -- analogs & derivatives KW - Asparagine -- chemistry KW - Magnetic Resonance Spectroscopy KW - Catalysis KW - Aspartic Acid -- genetics KW - Oxidoreductases -- genetics KW - Peroxiredoxins -- chemistry KW - Oxidoreductases -- metabolism KW - Peroxiredoxins -- metabolism KW - Models, Molecular KW - Peroxiredoxins -- genetics KW - Adenosine Triphosphate -- metabolism KW - Oxidoreductases -- chemistry KW - Models, Chemical KW - Aspartic Acid -- chemistry KW - Adenosine Triphosphate -- chemistry UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68252271?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Biochemistry&rft.atitle=Mutagenesis+and+modeling+of+the+peroxiredoxin+%28Prx%29+complex+with+the+NMR+structure+of+ATP-bound+human+sulfiredoxin+implicate+aspartate+187+of+Prx+I+as+the+catalytic+residue+in+ATP+hydrolysis.&rft.au=Lee%2C+Duck-Yeon%3BPark%2C+Sung+Jun%3BJeong%2C+Woojin%3BSung%2C+Ho+Jin%3BOho%2C+Taena%3BWu%2C+Xiongwu%3BRhee%2C+Sue+Goo%3BGruschus%2C+James+M&rft.aulast=Lee&rft.aufirst=Duck-Yeon&rft.date=2006-12-26&rft.volume=45&rft.issue=51&rft.spage=15301&rft.isbn=&rft.btitle=&rft.title=Biochemistry&rft.issn=1520-4995&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2008-01-11 N1 - Date created - 2007-03-06 N1 - Date revised - 2017-01-13 N1 - Genetic sequence - 1YZS; PDB; 2B6F N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Selection of a novel gp41-specific HIV-1 neutralizing human antibody by competitive antigen panning AN - 19531787; 7245976 AB - The HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against Env-based antigens has resulted mostly in selection of anti-gp120 antibodies. Native gp41 in the absence of gp120 is unstable. The use of gp41 fragments as antigens has resulted in selection of antibodies with only relatively modest neutralizing activity. To enhance selection of antibodies specific for gp41 in the context of the whole Env we developed a methodology termed competitive antigen panning (CAP). Using CAP, we identified a novel gp41-specific human monoclonal antibody (hmAb), m48, from an immune library derived from long-term nonprogressors with high titers of broadly cross-reactive neutralizing antibodies (bcnAbs). Selection of m48 was only successful using CAP and not through the conventional pre-incubation methodology. In assays based on spreading infection in peripheral blood mononuclear cells (PBMCs) m48 neutralized a panel of HIV-1 primary isolates from different clades more potently than the well-characterized broadly cross-reactive HIV-1-neutralizing antibodies IgG1 4E10 and Fab Z13. These results may have implications for the selection of novel gp41-specific bcnAbs and other antibodies, and for the development of HIV-1 inhibitors and vaccine immunogens. JF - Journal of Immunological Methods AU - Zhang, MY AU - Choudhry, V AU - Sidorov, IA AU - Tenev, V AU - Vu, B K AU - Choudhary, A AU - Lu, H AU - Stiegler, G M AU - Katinger, HWD AU - Jiang, S AU - Broder, C C AU - Dimitrov, D S AD - CCRNP, CCR, NCI-Frederick, NIH, Frederick, Maryland, USA, zhangm@ncifcrf.gov Y1 - 2006/12/20/ PY - 2006 DA - 2006 Dec 20 SP - 21 EP - 30 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 317 IS - 1-2 SN - 0022-1759, 0022-1759 KW - Immunology Abstracts; Biotechnology and Bioengineering Abstracts KW - Spreading KW - Infection KW - Glycoprotein gp120 KW - Peripheral blood mononuclear cells KW - Envelopes KW - Human immunodeficiency virus 1 KW - antibody libraries KW - glycoprotein gp41 KW - Monoclonal antibodies KW - Panning KW - Immunoglobulin G KW - Vaccines KW - Fab KW - F 06900:Methods KW - W 30900:Methods UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19531787?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Immunological+Methods&rft.atitle=Selection+of+a+novel+gp41-specific+HIV-1+neutralizing+human+antibody+by+competitive+antigen+panning&rft.au=Zhang%2C+MY%3BChoudhry%2C+V%3BSidorov%2C+IA%3BTenev%2C+V%3BVu%2C+B+K%3BChoudhary%2C+A%3BLu%2C+H%3BStiegler%2C+G+M%3BKatinger%2C+HWD%3BJiang%2C+S%3BBroder%2C+C+C%3BDimitrov%2C+D+S&rft.aulast=Zhang&rft.aufirst=MY&rft.date=2006-12-20&rft.volume=317&rft.issue=1-2&rft.spage=21&rft.isbn=&rft.btitle=&rft.title=Journal+of+Immunological+Methods&rft.issn=00221759&rft_id=info:doi/10.1016%2Fj.jim.2006.09.016 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-02-01 N1 - Last updated - 2011-12-13 N1 - SubjectsTermNotLitGenreText - Human immunodeficiency virus 1; glycoprotein gp41; Panning; Glycoprotein gp120; Spreading; Fab; Vaccines; Peripheral blood mononuclear cells; Monoclonal antibodies; antibody libraries; Infection; Immunoglobulin G; Envelopes DO - http://dx.doi.org/10.1016/j.jim.2006.09.016 ER - TY - JOUR T1 - Asbestos redirects nitric oxide signaling through rapid catalytic conversion to nitrite. AN - 68258126; 17178853 AB - Asbestos exposure is strongly associated with the development of malignant mesothelioma, yet the mechanistic basis of this observation has not been resolved. Carcinogenic transformation or tumor progression mediated by asbestos may be related to the generation of free radical species and perturbation of cell signaling and transcription factors. We report here that exposure of human mesothelioma or lung carcinoma cells to nitric oxide (NO) in the presence of crocidolite asbestos resulted in a marked decrease in intracellular nitrosation and diminished NO-induced posttranslational modifications of tumor-associated proteins (hypoxia-inducible factor-1alpha and p53). Crocidolite rapidly scavenged NO with concomitant conversion to nitrite (NO(2)(-)). Crocidolite also catalyzed the nitration of cellular proteins in the presence of NO(2)(-) and hydrogen peroxide. Nitrated protein adducts are a prominent feature of asbestos-induced lung injury. These data highlight the ability of asbestos to induce phenotypic cellular changes through two processes: (a) by directly reducing bioactive NO levels and preventing its subsequent interaction with target molecules and (b) by increasing oxidative damage and protein modifications through NO(2) production and 3-nitrotyrosine formation. JF - Cancer research AU - Thomas, Douglas D AU - Espey, Michael G AU - Pociask, Derek A AU - Ridnour, Lisa A AU - Donzelli, Sonia AU - Wink, David A AD - Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA. thomasdo@mail.nih.gov Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 11600 EP - 11604 VL - 66 IS - 24 SN - 0008-5472, 0008-5472 KW - HIF1A protein, human KW - 0 KW - Hypoxia-Inducible Factor 1, alpha Subunit KW - Nitrites KW - Serum Albumin, Bovine KW - Tumor Suppressor Protein p53 KW - Asbestos KW - 1332-21-4 KW - Phosphoserine KW - 17885-08-4 KW - Nitric Oxide KW - 31C4KY9ESH KW - Index Medicus KW - Animals KW - Hypoxia-Inducible Factor 1, alpha Subunit -- drug effects KW - Cattle KW - Humans KW - Tumor Suppressor Protein p53 -- drug effects KW - Nitrites -- metabolism KW - Signal Transduction -- drug effects KW - Serum Albumin, Bovine -- drug effects KW - Asbestos -- pharmacology KW - Nitric Oxide -- physiology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68258126?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Cancer+research&rft.atitle=Asbestos+redirects+nitric+oxide+signaling+through+rapid+catalytic+conversion+to+nitrite.&rft.au=Thomas%2C+Douglas+D%3BEspey%2C+Michael+G%3BPociask%2C+Derek+A%3BRidnour%2C+Lisa+A%3BDonzelli%2C+Sonia%3BWink%2C+David+A&rft.aulast=Thomas&rft.aufirst=Douglas&rft.date=2006-12-15&rft.volume=66&rft.issue=24&rft.spage=11600&rft.isbn=&rft.btitle=&rft.title=Cancer+research&rft.issn=00085472&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-25 N1 - Date created - 2006-12-20 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Synergism between CpG-containing oligodeoxynucleotides and IL-2 causes dramatic enhancement of vaccine-elicited CD8+ T cell responses. AN - 68208541; 17142789 AB - Novel anticancer vaccination regimens that can elicit large numbers of Ag-specific T cells are needed. When we administered therapeutic vaccines containing the MHC class I-presented self-peptide tyrosinase-related protein (TRP)-2(180-188) and CpG-containing oligodeoxynucleotides (CpG ODN) to mice, growth of the TRP-2-expressing B16F1 melanoma was not inhibited compared with growth in mice that received control vaccinations. When we added systemic IL-2 to the TRP-2(180-188) plus CpG ODN vaccines, growth of B16F1 was inhibited in a CD8-dependent, epitope-specific manner. Vaccines containing TRP-2(180-188) without CpG ODN did not cause epitope-specific tumor growth inhibition when administered with IL-2. The antitumor efficacy of the different regimens correlated with their ability to elicit TRP-2(180-188)-specific CD8+ T cell responses. When we administered TRP-2(180-188) plus CpG ODN-containing vaccines with systemic IL-2, 18.2% of CD8+ T cells were specific for TRP-2(180-188). Identical TRP-2(180-188) plus CpG ODN vaccines given without IL-2 elicited a TRP-2(180-188)-specific CD8+ T cell response of only 1.1% of CD8+ T cells. Vaccines containing TRP-2(180-188) without CpG ODN elicited TRP-2(180-188)-specific responses of 2.8% of CD8+ T cells when administered with IL-2. There was up to a 221-fold increase in the absolute number of TRP-2(180-188)-specific CD8+ T cells when IL-2 was added to TRP-2(180-188) plus CpG ODN-containing vaccines. Peptide plus CpG ODN vaccines administered with IL-2 generated epitope-specific CD8+ T cells by a mechanism that depended on endogenous IL-6. This is the first report of synergism between CpG ODN and IL-2. This synergism caused a striking increase in vaccine-elicited CD8+ T cells and led to epitope-specific antitumor immunity. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Kochenderfer, James N AU - Chien, Christopher D AU - Simpson, Jessica L AU - Gress, Ronald E AD - Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA. kochendj@mail.nih.gov Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 8860 EP - 8873 VL - 177 IS - 12 SN - 0022-1767, 0022-1767 KW - Antigens, Neoplasm KW - 0 KW - Cancer Vaccines KW - Epitopes, T-Lymphocyte KW - Interleukin-2 KW - Oligodeoxyribonucleotides KW - Intramolecular Oxidoreductases KW - EC 5.3.- KW - dopachrome isomerase KW - EC 5.3.3.12 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - CpG Islands KW - Mice KW - Intramolecular Oxidoreductases -- immunology KW - Immunity -- drug effects KW - Drug Synergism KW - Interleukin-2 -- pharmacology KW - Cancer Vaccines -- immunology KW - CD8-Positive T-Lymphocytes -- drug effects KW - Oligodeoxyribonucleotides -- therapeutic use KW - Cancer Vaccines -- chemistry KW - CD8-Positive T-Lymphocytes -- immunology KW - Interleukin-2 -- therapeutic use KW - Oligodeoxyribonucleotides -- pharmacology KW - Melanoma, Experimental -- therapy UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68208541?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Synergism+between+CpG-containing+oligodeoxynucleotides+and+IL-2+causes+dramatic+enhancement+of+vaccine-elicited+CD8%2B+T+cell+responses.&rft.au=Kochenderfer%2C+James+N%3BChien%2C+Christopher+D%3BSimpson%2C+Jessica+L%3BGress%2C+Ronald+E&rft.aulast=Kochenderfer&rft.aufirst=James&rft.date=2006-12-15&rft.volume=177&rft.issue=12&rft.spage=8860&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-16 N1 - Date created - 2006-12-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Characterization of the B cell epitopes associated with a truncated form of Pseudomonas exotoxin (PE38) used to make immunotoxins for the treatment of cancer patients. AN - 68208496; 17142785 AB - Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell leukemia and also has activity in other hemological malignancies; however, a neutralizing Ab response to PE38 in patients with solid tumors prevents repeated treatments to maximize the benefit. In this study, we analyze the murine Ab response as a model to study the B cell epitopes associated with PE38. Sixty distinct mAbs to PE38 were characterized. Mutual competitive binding of the mAbs indicated the presence of 7 major epitope groups and 13 subgroups. The competition pattern indicated that the epitopes are discrete and could not be reproduced using a computer simulation program that created epitopes out of random surface residues on PE38. Using sera from immunotoxin-treated patients, the formation of human Abs to each of the topographical epitopes was demonstrated. One epitope subgroup, E1a, was identified as the principal neutralizing epitope. The location of each epitope on PE38 was determined by preparing 41 mutants of PE38 in which bulky surface residues were mutated to either alanine or glycine. All 7 major epitope groups and 9 of 13 epitope subgroups were identified by 14 different mutants and these retained high cytotoxic activity. Our results indicate that a relatively small number of discrete immunogenic sites are associated with PE38, most of which can be eliminated by point mutations. JF - Journal of immunology (Baltimore, Md. : 1950) AU - Onda, Masanori AU - Nagata, Satoshi AU - FitzGerald, David J AU - Beers, Richard AU - Fisher, Robert J AU - Vincent, James J AU - Lee, Byungkook AU - Nakamura, Michihiro AU - Hwang, Jaulang AU - Kreitman, Robert J AU - Hassan, Raffit AU - Pastan, Ira AD - Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892, USA. Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 8822 EP - 8834 VL - 177 IS - 12 SN - 0022-1767, 0022-1767 KW - Antibodies, Monoclonal KW - 0 KW - Bacterial Toxins KW - Epitopes, B-Lymphocyte KW - Exotoxins KW - Immunoglobulin Fragments KW - Immunotoxins KW - Recombinant Fusion Proteins KW - Virulence Factors KW - immunoglobulin Fv KW - ADP Ribose Transferases KW - EC 2.4.2.- KW - toxA protein, Pseudomonas aeruginosa KW - EC 2.4.2.31 KW - Abridged Index Medicus KW - Index Medicus KW - Animals KW - Humans KW - Enzyme-Linked Immunosorbent Assay KW - Antibody Formation KW - Mice KW - Neoplasms -- drug therapy KW - Exotoxins -- genetics KW - ADP Ribose Transferases -- immunology KW - Virulence Factors -- therapeutic use KW - Virulence Factors -- genetics KW - Pseudomonas KW - Bacterial Toxins -- immunology KW - Exotoxins -- immunology KW - Virulence Factors -- immunology KW - ADP Ribose Transferases -- therapeutic use KW - ADP Ribose Transferases -- genetics KW - Bacterial Toxins -- genetics KW - Bacterial Toxins -- therapeutic use KW - Epitopes, B-Lymphocyte -- immunology KW - Exotoxins -- therapeutic use KW - Epitope Mapping UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68208496?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.atitle=Characterization+of+the+B+cell+epitopes+associated+with+a+truncated+form+of+Pseudomonas+exotoxin+%28PE38%29+used+to+make+immunotoxins+for+the+treatment+of+cancer+patients.&rft.au=Onda%2C+Masanori%3BNagata%2C+Satoshi%3BFitzGerald%2C+David+J%3BBeers%2C+Richard%3BFisher%2C+Robert+J%3BVincent%2C+James+J%3BLee%2C+Byungkook%3BNakamura%2C+Michihiro%3BHwang%2C+Jaulang%3BKreitman%2C+Robert+J%3BHassan%2C+Raffit%3BPastan%2C+Ira&rft.aulast=Onda&rft.aufirst=Masanori&rft.date=2006-12-15&rft.volume=177&rft.issue=12&rft.spage=8822&rft.isbn=&rft.btitle=&rft.title=Journal+of+immunology+%28Baltimore%2C+Md.+%3A+1950%29&rft.issn=00221767&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-16 N1 - Date created - 2006-12-04 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Human papillomavirus prevalence in women who have and have not undergone hysterectomies. AN - 68157809; 17109342 AB - We compared human papillomavirus (HPV) prevalence in an age-stratified random sample of women who have undergone a hysterectomy (WH) (n=573) with the HPV prevalence in age-matched women with intact cervices (women who have not undergone a hysterectomy [WNH]) (n=581) participating in a study at Kaiser Permanente in Portland, Oregon. Testing cervicovaginal lavage fluids for >40 HPV genotypes using an MY09/11 L1 consensus primer polymerase chain reaction method, we found no statistical differences in the prevalence of HPV (16% for WNH vs. 13.9% for WH) or carcinogenic HPV (6.5% for WNH vs. 4.5% for WH) between the 2 groups of women. Although WH have a similar prevalence of carcinogenic HPV infection, compared with WNH without a cervix, they have minimal risk of HPV-induced cancer and are unlikely to benefit from HPV testing. JF - The Journal of infectious diseases AU - Castle, Philip E AU - Schiffman, Mark AU - Glass, Andrew G AU - Rush, Brenda B AU - Scott, David R AU - Wacholder, Sholom AU - Dunn, Anne AU - Burk, Robert D AD - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Rockville, MD, USA. castlep@mail.nih.gov Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 1702 EP - 1705 VL - 194 IS - 12 SN - 0022-1899, 0022-1899 KW - DNA Primers KW - 0 KW - DNA, Viral KW - Abridged Index Medicus KW - Index Medicus KW - Human papillomavirus 16 -- isolation & purification KW - Humans KW - Vaginal Douching KW - Aged KW - Oregon KW - Polymerase Chain Reaction KW - Adult KW - Middle Aged KW - Human papillomavirus 16 -- genetics KW - DNA, Viral -- genetics KW - Species Specificity KW - Female KW - Papillomaviridae -- classification KW - Hysterectomy KW - Papillomaviridae -- isolation & purification KW - Vagina -- virology KW - Papillomavirus Infections -- virology KW - Uterine Cervical Neoplasms -- surgery KW - Papillomaviridae -- genetics KW - Papillomavirus Infections -- surgery KW - Uterine Cervical Neoplasms -- virology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68157809?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=The+Journal+of+infectious+diseases&rft.atitle=Human+papillomavirus+prevalence+in+women+who+have+and+have+not+undergone+hysterectomies.&rft.au=Castle%2C+Philip+E%3BSchiffman%2C+Mark%3BGlass%2C+Andrew+G%3BRush%2C+Brenda+B%3BScott%2C+David+R%3BWacholder%2C+Sholom%3BDunn%2C+Anne%3BBurk%2C+Robert+D&rft.aulast=Castle&rft.aufirst=Philip&rft.date=2006-12-15&rft.volume=194&rft.issue=12&rft.spage=1702&rft.isbn=&rft.btitle=&rft.title=The+Journal+of+infectious+diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-01-17 N1 - Date created - 2006-11-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Buprenorphine and HIV primary care: new opportunities for integrated treatment. AN - 68151099; 17109302 AB - Drug abuse and infection with human immunodeficiency virus (HIV) are associated with high rates of morbidity and mortality, but, because of medical, social, and legal factors, opiate addiction/dependence is a major obstacle to successful treatment of disease--for example, treatment of acquired immunodeficiency syndrome (AIDS) with highly active antiretroviral therapy. In an effort to improve the opportunity for treatment of drug abuse and HIV infection, the Forum for Collaborative HIV Research, in collaboration with the Substance Abuse and Mental Health Services Administration, the National Institute on Drug Abuse, the Centers for Disease Control and Prevention, and other agencies, presented a workshop entitled "Buprenorphine in the Primary HIV Care Setting." Participants reviewed and discussed current issues, such as the introduction of and sources for the provision of buprenorphine in HIV primary care settings and strategies for integrating treatment of HIV-infected drug abusers, all of which are covered in this supplement. JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America AU - Khalsa, Jag AU - Vocci, Francis AU - Altice, Frederick AU - Fiellin, David AU - Miller, Veronica AD - National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD 20892-9551, USA. jk98p@nih.gov Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - S169 EP - S172 VL - 43 Suppl 4 KW - Narcotic Antagonists KW - 0 KW - Buprenorphine KW - 40D3SCR4GZ KW - Index Medicus KW - United States KW - Humans KW - Health Services Research KW - Treatment Outcome KW - Incidence KW - Follow-Up Studies KW - Primary Prevention -- methods KW - Antiretroviral Therapy, Highly Active -- methods KW - Male KW - Female KW - Survival Analysis KW - Risk Assessment KW - Opioid-Related Disorders -- mortality KW - Buprenorphine -- therapeutic use KW - Narcotic Antagonists -- therapeutic use KW - HIV Infections -- drug therapy KW - HIV Infections -- mortality KW - Primary Health Care -- methods KW - Opioid-Related Disorders -- drug therapy KW - Delivery of Health Care, Integrated -- methods KW - HIV Infections -- diagnosis UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68151099?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.atitle=Buprenorphine+and+HIV+primary+care%3A+new+opportunities+for+integrated+treatment.&rft.au=Khalsa%2C+Jag%3BVocci%2C+Francis%3BAltice%2C+Frederick%3BFiellin%2C+David%3BMiller%2C+Veronica&rft.aulast=Khalsa&rft.aufirst=Jag&rft.date=2006-12-15&rft.volume=43+Suppl+4&rft.issue=&rft.spage=S169&rft.isbn=&rft.btitle=&rft.title=Clinical+infectious+diseases+%3A+an+official+publication+of+the+Infectious+Diseases+Society+of+America&rft.issn=1537-6591&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-04-13 N1 - Date created - 2006-11-19 N1 - Date revised - 2017-01-13 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - Phase 1 Safety and Immunogenicity Evaluation of a Multiclade HIV-1 Candidate Vaccine Delivered by a Replication-Defective Recombinant Adenovirus Vector AN - 19522515; 7211371 AB - Background. The development of an effective human immunodeficiency virus (HIV) vaccine is a high global priority. Here, we report the safety, tolerability, and immunogenicity of a replication-defective recombinant adenovirus serotype 5 (rAd5) vector HIV-1 candidate vaccine. Methods. The vaccine is a mixture of 4 rAd5 vectors that express HIV-1 subtype B Gag-Pol fusion protein and envelope (Env) from subtypes A, B, and C. Healthy, uninfected adults were randomized to receive 1 intramuscular injection of placebo (n = 6) or vaccine at dose levels of 10 super(9) (n = 10), 10 super(10) (n = 10), or 10 super(11) (n = 10) particle units and were followed for 24 weeks to assess immunogenicity and safety. Results. The vaccine was well tolerated but was associated with more reactogenicity at the highest dose. At week 4, vaccine antigen-specific T cell responses were detected in 28 (93.3%) and 18 (60%) of 30 vaccine recipients for CD4 super(+) and CD8 super(+) T cells, respectively, by intracellular cytokine staining assay and in 22 (73%) of 30 vaccine recipients by enzyme-linked immunospot assay. Env-specific antibody responses were detected in 15 (50%) of 30 vaccine recipients by enzyme-linked immunosorbant assay and in 28 (93.3%) of 30 vaccine recipients by immunoprecipitation followed by Western blotting. No neutralizing antibody was detected. Conclusions. A single injection induced HIV-1 antigen-specific CD4 T cell, CD8 super(+) T cell, and antibody responses in the majority of vaccine recipients. This multiclade rAd5 HIV-1 vaccine is now being evaluated in combination with a multiclade HIV-1 DNA plasmid vaccine. JF - Journal of Infectious Diseases AU - Catanzaro, A T AU - Koup, R A AU - Roederer, M AU - Bailer, R T AU - Enama, ME AU - Moodie, Z AU - Gu, L AU - Martin, JE AU - Novik, L AU - Chakrabarti, B K AU - Butman, B T AU - Gall, JGD AU - King, C R AU - Andrews, CA AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 1638 EP - 1649 VL - 194 IS - 12 SN - 0022-1899, 0022-1899 KW - Health & Safety Science Abstracts; Virology & AIDS Abstracts; Immunology Abstracts KW - Western blotting KW - vaccines KW - Enzyme-linked immunosorbent assay KW - Serotypes KW - Adenovirus KW - Immunoprecipitation KW - CD8 antigen KW - Plasmids KW - Expression vectors KW - CD4 antigen KW - Antibodies KW - Envelopes KW - DNA vaccines KW - Immunogenicity KW - Human immunodeficiency virus 1 KW - DNA KW - Lymphocytes T KW - Proteins KW - Cytokines KW - Fusion protein KW - Vaccines KW - Side effects KW - V 22360:AIDS and HIV KW - F 06905:Vaccines KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19522515?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Ahealthsafetyabstracts&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Phase+1+Safety+and+Immunogenicity+Evaluation+of+a+Multiclade+HIV-1+Candidate+Vaccine+Delivered+by+a+Replication-Defective+Recombinant+Adenovirus+Vector&rft.au=Catanzaro%2C+A+T%3BKoup%2C+R+A%3BRoederer%2C+M%3BBailer%2C+R+T%3BEnama%2C+ME%3BMoodie%2C+Z%3BGu%2C+L%3BMartin%2C+JE%3BNovik%2C+L%3BChakrabarti%2C+B+K%3BButman%2C+B+T%3BGall%2C+JGD%3BKing%2C+C+R%3BAndrews%2C+CA&rft.aulast=Catanzaro&rft.aufirst=A&rft.date=2006-12-15&rft.volume=194&rft.issue=12&rft.spage=1638&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Western blotting; Enzyme-linked immunosorbent assay; Serotypes; Immunoprecipitation; CD8 antigen; Plasmids; Expression vectors; Antibodies; CD4 antigen; Envelopes; DNA vaccines; Immunogenicity; Lymphocytes T; Cytokines; Vaccines; Fusion protein; vaccines; DNA; Proteins; Side effects; Human immunodeficiency virus 1; Adenovirus ER - TY - JOUR T1 - Escherichia coli BL21(DE3) chromosome contains a group II capsular gene cluster AN - 19520002; 7203397 AB - During our study of de novo synthesis of Escherichia coli K1 capsular polysaccharides, we found that E. coli BL21(DE3) has a capsular gene cluster, similar to those of group II capsular E. coli strains. Analysis of the nucleotide sequence of the E. coli BL21(DE3) gene cluster showed homologues to all group II regions 1 and 3 genes and the presence of an IS1 element in one of the region 2 ORFs, which likely prevents capsule expression. Complementation analysis showed that region 1 and 3 genes encode functional proteins that are sufficient for the export of newly synthesized polysaccharide. The gene products of Bl21(DE3) kpsC and kpsS supported in vitro de novo synthesis of K1 polysaccharide when co-expressed with K1 NeuE and NeuS. Sequence homology between BL21(DE3) region 2 open reading frames and capsule-related genes in other bacteria such as Haemophilus influenzae serotype b, suggests that the encapsulated ancestor of BL21(DE3) may have produced a ribose/ribitol-phosphate containing polysaccharide. JF - Gene AU - Andreishcheva, EN AU - Vann, W F AD - Center for Biologics Evaluation and Research, Building 29, Room 103, 8800 Rockville Pike, Bethesda, MD 20892, USA, wvann@helix.nih.gov Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 113 EP - 119 PB - Elsevier Science B.V., P.O. Box 211 Amsterdam 1000 AE Netherlands, [mailto:nlinfo-f@elsevier.nl], [URL:http://www.elsevier.nl/] VL - 384 SN - 0378-1119, 0378-1119 KW - Microbiology Abstracts B: Bacteriology; Genetics Abstracts KW - Haemophilus influenzae KW - Serotypes KW - Nucleotide sequence KW - Ribose KW - Polysaccharides KW - Chromosomes KW - Complementation KW - Homology KW - Neu protein KW - Gene clusters KW - Escherichia coli KW - Open reading frames KW - J 02310:Genetics & Taxonomy KW - G 07770:Bacteria UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19520002?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Gene&rft.atitle=Escherichia+coli+BL21%28DE3%29+chromosome+contains+a+group+II+capsular+gene+cluster&rft.au=Andreishcheva%2C+EN%3BVann%2C+W+F&rft.aulast=Andreishcheva&rft.aufirst=EN&rft.date=2006-12-15&rft.volume=384&rft.issue=&rft.spage=113&rft.isbn=&rft.btitle=&rft.title=Gene&rft.issn=03781119&rft_id=info:doi/10.1016%2Fj.gene.2006.07.020 LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Chromosomes; Complementation; Serotypes; Homology; Nucleotide sequence; Neu protein; Ribose; Gene clusters; Polysaccharides; Open reading frames; Haemophilus influenzae; Escherichia coli DO - http://dx.doi.org/10.1016/j.gene.2006.07.020 ER - TY - JOUR T1 - Human Cytolytic T Cell Recognition of Yersinia pestis Virulence Proteins That Target Innate Immune Responses AN - 19519291; 7211385 AB - Cell contact by the plague bacterium Yersinia pestis initiates the injection of several virulence factors that target biochemical pathways critical for host clearance of bacteria. Despite this impairment of innate immunity, it is unclear whether antigen recognition by T cells is equally affected. We present evidence that human cytolytic T cells respond to Y. pestis virulence proteins presented by infected monocytes and dendritic cells. These T cell antigens consisted of a panel of proteins encoded by pCD1, a 70-kDa plasmid that harbors virulence factors and transport proteins of the cell contact-dependent, type III secretion system. Infected cells retained the ability to process and present tetanus toxoid to T cells, which indicates that responses to unrelated antigens were also maintained. Our results indicate that T cell immunity remains functional during Y. pestis infection, which thus suggests the potential benefits of therapeutic vaccination and strategies that emphasize the inclusion of cytotoxic T lymphocyte responses. JF - Journal of Infectious Diseases AU - Saikh, K U AU - Kissner, T L AU - Dyas, B AU - Tropea, JE AU - Waugh, D S AU - Ulrich, R G AD - Department of Immunology, United States Army Medical Research Institute of Infectious Diseases, and Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, Frederick, Maryland, USA Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 1753 EP - 1760 VL - 194 IS - 12 SN - 0022-1899, 0022-1899 KW - Microbiology Abstracts B: Bacteriology; Immunology Abstracts KW - virulence factors KW - Yersinia pestis KW - Toxoids KW - Plasmids KW - Tetanus KW - Infection KW - Vaccination KW - Virulence KW - Dendritic cells KW - Cytotoxicity KW - Lymphocytes T KW - Monocytes KW - Plague KW - F 06905:Vaccines KW - J 02350:Immunology UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19519291?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Amicrobiologyb&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Human+Cytolytic+T+Cell+Recognition+of+Yersinia+pestis+Virulence+Proteins+That+Target+Innate+Immune+Responses&rft.au=Saikh%2C+K+U%3BKissner%2C+T+L%3BDyas%2C+B%3BTropea%2C+JE%3BWaugh%2C+D+S%3BUlrich%2C+R+G&rft.aulast=Saikh&rft.aufirst=K&rft.date=2006-12-15&rft.volume=194&rft.issue=12&rft.spage=1753&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Virulence; Dendritic cells; Cytotoxicity; virulence factors; Lymphocytes T; Toxoids; Plague; Monocytes; Infection; Tetanus; Plasmids; Vaccination; Yersinia pestis ER - TY - JOUR T1 - Phase 1 Safety and Immunogenicity Evaluation of a Multiclade HIV-1 DNA Candidate Vaccine AN - 19515759; 7211372 AB - Background. Gene-based vaccine delivery is an important strategy in the development of a preventive vaccine for acquired immunodeficiency syndrome (AIDS). Vaccine Research Center (VRC) 004 is the first phase 1 dose-escalation study of a multiclade HIV-1 DNA vaccine. Methods. VRC-HIVDNA009-00-VP is a 4-plasmid mixture encoding subtype B Gag-Pol-Nef fusion protein and modified envelope (Env) constructs from subtypes A, B, and C. Fifty healthy, uninfected adults were randomized to receive either placebo (n = 10) or study vaccine at 2 mg (n = 5), 4 mg (n = 20), or 8 mg (n = 15) by needle-free intramuscular injection. Humoral responses (measured by enzyme-linked immunosorbant assay, Western blotting, and neutralization assay) and T cell responses (measured by enzyme-linked immunospot assay and intracellular cytokine staining after stimulation with antigen-specific peptide pools) were measured. Results. The vaccine was well tolerated and induced cellular and humoral responses. The maximal CD4 super(+) and CD8 super(+) T cell responses occurred after 3 injections and were in response to Env peptide pools. The pattern of cytokine expression by vaccine-induced HIV-specific T cells evolved over time, with a diminished frequency of interferon- gamma -producing T cells and an increased frequency of interleukin-2-producing T cells at 1 year. Conclusions. DNA vaccination induced antibody to and T cell responses against 3 major HIV-1 subtypes and will be further evaluated as a potential component of a preventive AIDS vaccine regimen. JF - Journal of Infectious Diseases AU - Graham, B S AU - Koup, R A AU - Roederer, M AU - Bailer, R T AU - Enama, ME AU - Moodie, Z AU - Martin, JE AU - McCluskey, M M AU - Chakrabarti, B K AU - Lamoreaux, L AU - Andrews, CA AU - Gomez, P L AU - Mascola, J R AU - Nabel, G J AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Y1 - 2006/12/15/ PY - 2006 DA - 2006 Dec 15 SP - 1650 EP - 1660 VL - 194 IS - 12 SN - 0022-1899, 0022-1899 KW - HIV-1 KW - Biochemistry Abstracts 2: Nucleic Acids; Health & Safety Science Abstracts; Virology & AIDS Abstracts; Immunology Abstracts; Biotechnology and Bioengineering Abstracts KW - Western blotting KW - vaccines KW - Enzyme-linked immunosorbent assay KW - Acquired immune deficiency syndrome KW - Immunodeficiency KW - CD8 antigen KW - Vaccination KW - CD4 antigen KW - Antibodies KW - Envelopes KW - DNA vaccines KW - Immunogenicity KW - Human immunodeficiency virus 1 KW - DNA KW - Lymphocytes T KW - Proteins KW - Cytokines KW - Fusion protein KW - Vaccines KW - Neutralization KW - V 22360:AIDS and HIV KW - F 06905:Vaccines KW - W 30915:Pharmaceuticals & Vaccines KW - N 14810:Methods KW - H 4000:Food and Drugs UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/19515759?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Abiotechresearch&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Journal+of+Infectious+Diseases&rft.atitle=Phase+1+Safety+and+Immunogenicity+Evaluation+of+a+Multiclade+HIV-1+DNA+Candidate+Vaccine&rft.au=Graham%2C+B+S%3BKoup%2C+R+A%3BRoederer%2C+M%3BBailer%2C+R+T%3BEnama%2C+ME%3BMoodie%2C+Z%3BMartin%2C+JE%3BMcCluskey%2C+M+M%3BChakrabarti%2C+B+K%3BLamoreaux%2C+L%3BAndrews%2C+CA%3BGomez%2C+P+L%3BMascola%2C+J+R%3BNabel%2C+G+J&rft.aulast=Graham&rft.aufirst=B&rft.date=2006-12-15&rft.volume=194&rft.issue=12&rft.spage=1650&rft.isbn=&rft.btitle=&rft.title=Journal+of+Infectious+Diseases&rft.issn=00221899&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-01-01 N1 - Last updated - 2015-04-01 N1 - SubjectsTermNotLitGenreText - Western blotting; Acquired immune deficiency syndrome; Enzyme-linked immunosorbent assay; Immunodeficiency; CD8 antigen; Vaccination; Antibodies; CD4 antigen; Envelopes; DNA vaccines; Immunogenicity; Lymphocytes T; Cytokines; Vaccines; Fusion protein; vaccines; DNA; Proteins; Neutralization; Human immunodeficiency virus 1 ER - TY - CPAPER T1 - HER-2 Overexpression Promotes the Brain Metastasis of Breast Cancer. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40486489; 4481109 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Steeg, P S AU - Palmieri, D AU - Bronder, J L AU - Herring, J L AU - Halverson, D AU - Vega-Valle, E AU - Feigenbaum, L AU - Yoneda, T AU - Weil, R J AU - Stark, A M AU - Vortmeyer, A O AU - Kurek, R AU - Aldape, K Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Brain KW - Breast cancer KW - ErbB-2 protein KW - Metastases KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40486489?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=HER-2+Overexpression+Promotes+the+Brain+Metastasis+of+Breast+Cancer.&rft.au=Steeg%2C+P+S%3BPalmieri%2C+D%3BBronder%2C+J+L%3BHerring%2C+J+L%3BHalverson%2C+D%3BVega-Valle%2C+E%3BFeigenbaum%2C+L%3BYoneda%2C+T%3BWeil%2C+R+J%3BStark%2C+A+M%3BVortmeyer%2C+A+O%3BKurek%2C+R%3BAldape%2C+K&rft.aulast=Steeg&rft.aufirst=P&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Risk Factor- and Age-Specific Interactions Suggest different Etiologies for Early-Onset and Late-Onset Types of Breast Cancer T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40485369; 4481175 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Anderson, W F AU - Matsuno, R K AU - Yang, X AU - Sherman, M E AU - Garcia-Closas, M Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Etiology KW - Breast cancer KW - Age KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40485369?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Risk+Factor-+and+Age-Specific+Interactions+Suggest+different+Etiologies+for+Early-Onset+and+Late-Onset+Types+of+Breast+Cancer&rft.au=Anderson%2C+W+F%3BMatsuno%2C+R+K%3BYang%2C+X%3BSherman%2C+M+E%3BGarcia-Closas%2C+M&rft.aulast=Anderson&rft.aufirst=W&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Influence of Antibiotic Treatment on Breast Carcinoma Development and Mammary Gland Morphogenesis in Murine Models. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40483104; 4481169 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Rossini, A AU - Rumio, C AU - Sfondrini, L AU - Tagliabue, E AU - Morelli, D AU - Miceli, R AU - Mariani, L AU - Palazzo, M AU - Menard, S AU - Balsari, A Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Antibiotics KW - Mammary gland KW - Animal models KW - Breast carcinoma KW - Morphogenesis KW - Tumors KW - Glands KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40483104?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Influence+of+Antibiotic+Treatment+on+Breast+Carcinoma+Development+and+Mammary+Gland+Morphogenesis+in+Murine+Models.&rft.au=Rossini%2C+A%3BRumio%2C+C%3BSfondrini%2C+L%3BTagliabue%2C+E%3BMorelli%2C+D%3BMiceli%2C+R%3BMariani%2C+L%3BPalazzo%2C+M%3BMenard%2C+S%3BBalsari%2C+A&rft.aulast=Rossini&rft.aufirst=A&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Microarray Analysis of Resected Brain Metastases of Breast Cancer. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40482550; 4481110 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Steeg, P S AU - Palmieri, D AU - Bronder, J L AU - Halverson, D AU - Yoneda, T AU - Weil, R J AU - Stark, A M AU - Vortmeyer, A O AU - Kurek, R AU - Davis, S AU - Meltzer, P S Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Brain KW - Breast cancer KW - Metastases KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40482550?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Microarray+Analysis+of+Resected+Brain+Metastases+of+Breast+Cancer.&rft.au=Steeg%2C+P+S%3BPalmieri%2C+D%3BBronder%2C+J+L%3BHalverson%2C+D%3BYoneda%2C+T%3BWeil%2C+R+J%3BStark%2C+A+M%3BVortmeyer%2C+A+O%3BKurek%2C+R%3BDavis%2C+S%3BMeltzer%2C+P+S&rft.aulast=Steeg&rft.aufirst=P&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Gene Expression Profiling to Predict Response to Neoadjuvant Docetaxel and Capecitabine for Breast Cancer. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40468191; 4480902 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Korde, L AU - Zujewski, J AU - McShane, L AU - Lukes, L AU - Lebowitz, P AU - Finney, R AU - Hunter, K Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Breast cancer KW - Gene expression KW - Profiling KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40468191?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Gene+Expression+Profiling+to+Predict+Response+to+Neoadjuvant+Docetaxel+and+Capecitabine+for+Breast+Cancer.&rft.au=Korde%2C+L%3BZujewski%2C+J%3BMcShane%2C+L%3BLukes%2C+L%3BLebowitz%2C+P%3BFinney%2C+R%3BHunter%2C+K&rft.aulast=Korde&rft.aufirst=L&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Preliminary Evaluation of p53 Mutation Type, Tumor Characteristics and Clinical Response among Neoadjuvantly Treated Breast Cancer Patients in I-SPY1 (CALGB 150007/ACRIN 6657). T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40468161; 4480891 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Conway, K AU - Edmiston, S N AU - Tolbert, D AU - Moore, D Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Mutation KW - Breast cancer KW - P53 protein KW - Tumors KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40468161?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Preliminary+Evaluation+of+p53+Mutation+Type%2C+Tumor+Characteristics+and+Clinical+Response+among+Neoadjuvantly+Treated+Breast+Cancer+Patients+in+I-SPY1+%28CALGB+150007%2FACRIN+6657%29.&rft.au=Conway%2C+K%3BEdmiston%2C+S+N%3BTolbert%2C+D%3BMoore%2C+D&rft.aulast=Conway&rft.aufirst=K&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Are we Closer to Preventing Breast Cancer? Progress in Phase III Clinical Trials T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40466351; 4481477 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Ford, Leslie G Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Clinical trials KW - Breast cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40466351?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Are+we+Closer+to+Preventing+Breast+Cancer%3F+Progress+in+Phase+III+Clinical+Trials&rft.au=Ford%2C+Leslie+G&rft.aulast=Ford&rft.aufirst=Leslie&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Evaluation of E-Cadherin Expression and Lymphatic Involvement in Inflammatory Breast Cancer. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40465162; 4481022 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Portera, C C AU - Yang, S X AU - Wedam, S B AU - Nguyen, D AU - Vatas, U AU - Takikita, M AU - Hewitt, S M AU - Liewehr, D J AU - Steinberg, S M AU - Sherman, M AU - Levine, P H AU - Swain, S M Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Breast cancer KW - Inflammation KW - E-Cadherin KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40465162?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Evaluation+of+E-Cadherin+Expression+and+Lymphatic+Involvement+in+Inflammatory+Breast+Cancer.&rft.au=Portera%2C+C+C%3BYang%2C+S+X%3BWedam%2C+S+B%3BNguyen%2C+D%3BVatas%2C+U%3BTakikita%2C+M%3BHewitt%2C+S+M%3BLiewehr%2C+D+J%3BSteinberg%2C+S+M%3BSherman%2C+M%3BLevine%2C+P+H%3BSwain%2C+S+M&rft.aulast=Portera&rft.aufirst=C&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - A Sharp Decrease in Breast Cancer Incidence in the United States in 2003. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40464578; 4481435 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Ravdin, P M AU - Cronin, K A AU - Howlander, N AU - Chlebowski, R T AU - Berry, D A Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - USA KW - Breast cancer KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40464578?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=A+Sharp+Decrease+in+Breast+Cancer+Incidence+in+the+United+States+in+2003.&rft.au=Ravdin%2C+P+M%3BCronin%2C+K+A%3BHowlander%2C+N%3BChlebowski%2C+R+T%3BBerry%2C+D+A&rft.aulast=Ravdin&rft.aufirst=P&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Use of Dimethylbenzanthracene (DMBA) to Facilitate Carcinogenesis in Virgin Female FVB Mice Expressing MMTV-Neu or MMTV-Neu/p53 knock-Out. T2 - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AN - 40463068; 4480671 JF - 29th Annual San Antonio Breast Cancer Symposium (SABCS 2006) AU - Lubet, R A AU - Ruppert, M J AU - Juliana, M M AU - Lobo-Ruppert, S M AU - Grubbs, C J Y1 - 2006/12/14/ PY - 2006 DA - 2006 Dec 14 KW - Mice KW - Carcinogenesis KW - P53 protein KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/40463068?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.atitle=Use+of+Dimethylbenzanthracene+%28DMBA%29+to+Facilitate+Carcinogenesis+in+Virgin+Female+FVB+Mice+Expressing+MMTV-Neu+or+MMTV-Neu%2Fp53+knock-Out.&rft.au=Lubet%2C+R+A%3BRuppert%2C+M+J%3BJuliana%2C+M+M%3BLobo-Ruppert%2C+S+M%3BGrubbs%2C+C+J&rft.aulast=Lubet&rft.aufirst=R&rft.date=2006-12-14&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=29th+Annual+San+Antonio+Breast+Cancer+Symposium+%28SABCS+2006%29&rft.issn=&rft_id=info:doi/ L2 - http://www.sabcs.org/ProgramSchedule/ScheduleGlance.asp LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2008-05-27 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Integrating Transcriptome and Proteome Profiling at the Sympathoadrenal Synapse: Non-Linear Pathways for Intracellular Signaling in Neuroendocrine Cells. T2 - 2006 Mediterranean Conference of Neurosciences AN - 39337967; 4530559 JF - 2006 Mediterranean Conference of Neurosciences AU - Eiden, Lee E Y1 - 2006/12/13/ PY - 2006 DA - 2006 Dec 13 KW - Adrenal glands KW - Sympathetic nervous system KW - Intracellular signalling KW - Synapses KW - Profiling KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39337967?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2006+Mediterranean+Conference+of+Neurosciences&rft.atitle=Integrating+Transcriptome+and+Proteome+Profiling+at+the+Sympathoadrenal+Synapse%3A+Non-Linear+Pathways+for+Intracellular+Signaling+in+Neuroendocrine+Cells.&rft.au=Eiden%2C+Lee+E&rft.aulast=Eiden&rft.aufirst=Lee&rft.date=2006-12-13&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2006+Mediterranean+Conference+of+Neurosciences&rft.issn=&rft_id=info:doi/ L2 - http://www.ucam.ac.ma/neurosci.med2006/index_Eng.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - JOUR T1 - Renal atrial natriuretic peptide receptors binding properties and function are resistant to DOCA-salt-induced hypertension in rats. AN - 68238702; 16904201 AB - Atrial natriuretic peptide receptor types A (NPR-A) and C (NPR-C) binding properties and functional characteristics in renal glomeruli have been investigated in deoxycorticosterone acetate (DOCA)-treated hypertensive Wistar-Kyoto (WKY) rats and their respective controls. We found that DOCA administration had no significant effect on the maximum binding capacity or the affinity of renal NPR-A and NPR-C. NPR-C is involved in the regulation of cAMP production. Our results indicate that the cAMP production by NPR-C is not altered in DOCA-induced hypertension, since ANP(1-28), CNP(1-22) and C-ANP, which specifically bind to NPR-C, show a similar inhibitory effect on cAMP production stimulated by the physiological agonist histamine in glomeruli from DOCA-treated rats and controls. Finally, we have found that DOCA-induced hypertension does not modify NPR-A or NPR-C expression in rat glomerular membranes. These findings indicate that NPR-A and NPR-C binding properties and NPR-C-mediated inhibition of cAMP generation remain unaltered in DOCA-treated rats. JF - Regulatory peptides AU - Li, Xiaohong AU - Woodard, Geoffrey E AU - Brown, John AU - Rosado, Juan A AD - National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Rm 8C-208, 10 Center Drive, MSC 1752, Bethesda, MD 20892-1752, USA. GeoffreyW@intra.niddk.nih.gov Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 SP - 114 EP - 120 VL - 137 IS - 3 SN - 0167-0115, 0167-0115 KW - Desoxycorticosterone KW - 40GP35YQ49 KW - Atrial Natriuretic Factor KW - 85637-73-6 KW - Cyclic AMP KW - E0399OZS9N KW - Guanylate Cyclase KW - EC 4.6.1.2 KW - Receptors, Atrial Natriuretic Factor KW - atrial natriuretic factor receptor A KW - atrial natriuretic factor receptor C KW - Index Medicus KW - Rats KW - Guanylate Cyclase -- metabolism KW - Animals KW - Desoxycorticosterone -- toxicity KW - Rats, Inbred WKY KW - Binding, Competitive KW - Cyclic AMP -- metabolism KW - Atrial Natriuretic Factor -- metabolism KW - Kidney Glomerulus -- metabolism KW - Male KW - Kidney -- metabolism KW - Hypertension -- chemically induced KW - Receptors, Atrial Natriuretic Factor -- metabolism KW - Hypertension -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68238702?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Regulatory+peptides&rft.atitle=Renal+atrial+natriuretic+peptide+receptors+binding+properties+and+function+are+resistant+to+DOCA-salt-induced+hypertension+in+rats.&rft.au=Li%2C+Xiaohong%3BWoodard%2C+Geoffrey+E%3BBrown%2C+John%3BRosado%2C+Juan+A&rft.aulast=Li&rft.aufirst=Xiaohong&rft.date=2006-12-10&rft.volume=137&rft.issue=3&rft.spage=114&rft.isbn=&rft.btitle=&rft.title=Regulatory+peptides&rft.issn=01670115&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-03-07 N1 - Date created - 2006-12-12 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Erratum In: Regul Pept. 2008 Apr 10;147(1-3):111 N1 - Last updated - 2017-01-18 ER - TY - JOUR T1 - P-selectin activates integrin-mediated colon carcinoma cell adhesion to fibronectin. AN - 68214146; 17056038 AB - During hematogenous cancer metastasis, tumor cells separate from a primary mass, enter the bloodstream, disperse throughout the body, migrate across vessel walls, and generate distant colonies. The later steps of metastasis superficially resemble leukocyte extravasation, a process initiated by selectin-mediated cell tethering to the blood vessel wall followed by integrin-mediated arrest and transendothelial migration. Some cancer cells express P-selectin ligands and attach to immobilized P-selectin, suggesting that these cells can arrest in blood vessels using sequential selectin- and integrin-mediated adhesion, as do leukocytes. We hypothesize that selectin binding may regulate subsequent integrin-mediated steps in metastasis. Using a model system of cultured Colo 320 human colon adenocarcinoma cells incubated with soluble P-selectin-IgG chimeric protein, we have found that P-selectin can stimulate activation of the alpha(5)beta(1) integrin resulting in a specific increase of adhesion and spreading of these cells on fibronectin substrates. P-selectin binding also induced activation of p38 mitogen-activated protein kinase (p38 MAPK) and phosphatidylinositol 3-kinase (PI3-K). PI3-K inhibitors blocked P-selectin-mediated integrin activation, cell attachment, and cell spreading. Inhibition of p38 MAPK activation blocked cell spreading, but not cell attachment. P-selectin binding also resulted in formation of a signaling complex containing PI3-K and p38 MAPK. These results suggest that P-selectin binding to tumor cells can activate alpha(5)beta(1) integrin via PI3-K and p38 MAPK signaling pathways leading to increased cell adhesion. We propose that P-selectin ligands are important tumor cell signaling molecules that modulate integrin-mediated cell adhesion in the metastatic process. JF - Experimental cell research AU - Reyes-Reyes, Merit E AU - George, Margaret D AU - Roberts, John D AU - Akiyama, Steven K AD - Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA. Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 SP - 4056 EP - 4069 VL - 312 IS - 20 SN - 0014-4827, 0014-4827 KW - Fibronectins KW - 0 KW - Integrin alpha5beta1 KW - P-Selectin KW - Phosphatidylinositol 3-Kinases KW - EC 2.7.1.- KW - p38 Mitogen-Activated Protein Kinases KW - EC 2.7.11.24 KW - Index Medicus KW - MAP Kinase Signaling System KW - Phosphatidylinositol 3-Kinases -- metabolism KW - Enzyme Activation KW - Humans KW - Cell Line, Tumor KW - Protein Binding KW - p38 Mitogen-Activated Protein Kinases -- metabolism KW - Cell Adhesion KW - Adenocarcinoma -- metabolism KW - Integrin alpha5beta1 -- physiology KW - Integrin alpha5beta1 -- metabolism KW - Fibronectins -- metabolism KW - P-Selectin -- pharmacology KW - Colonic Neoplasms -- metabolism UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/68214146?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Atoxline&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.jtitle=Experimental+cell+research&rft.atitle=P-selectin+activates+integrin-mediated+colon+carcinoma+cell+adhesion+to+fibronectin.&rft.au=Reyes-Reyes%2C+Merit+E%3BGeorge%2C+Margaret+D%3BRoberts%2C+John+D%3BAkiyama%2C+Steven+K&rft.aulast=Reyes-Reyes&rft.aufirst=Merit&rft.date=2006-12-10&rft.volume=312&rft.issue=20&rft.spage=4056&rft.isbn=&rft.btitle=&rft.title=Experimental+cell+research&rft.issn=00144827&rft_id=info:doi/ LA - English DB - ProQuest Environmental Science Collection N1 - Date completed - 2007-02-13 N1 - Date created - 2006-12-05 N1 - Date revised - 2017-01-13 N1 - SuppNotes - Cited By: Clin Cancer Res. 2004 Jan 15;10(2):701-7 [14760093] Cancer Res. 2004 Apr 15;64(8):2743-50 [15087389] J Biol Chem. 2004 Apr 23;279(17):17897-904 [14973141] Crit Rev Oncol Hematol. 2004 May;50(2):87-100 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[9671523] Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9325-30 [9689079] FASEB J. 1998 Sep;12(12):1241-51 [9737727] Biochim Biophys Acta. 1998 Dec 8;1436(1-2):127-50 [9838078] Physiol Rev. 1999 Jan;79(1):181-213 [9922371] J Immunol. 1999 Mar 1;162(5):2850-7 [10072533] Science. 1999 Aug 13;285(5430):1028-32 [10446041] J Clin Invest. 2005 Feb;115(2):339-47 [15668738] Curr Opin Cell Biol. 2005 Apr;17(2):141-9 [15780590] Oncogene. 2005 Mar 24;24(13):2218-28 [15688026] Cancer Res. 1993 Jan 15;53(2):354-61 [7678075] Eur J Surg Oncol. 1993 Feb;19(1):50-60 [8436241] J Cell Biol. 1993 Apr;121(2):449-59 [7682218] Int J Cancer. 1993 Jul 30;54(6):972-7 [7687590] J Clin Invest. 1993 Aug;92(2):804-13 [7688763] J Biol Chem. 1993 Oct 15;268(29):21459-62 [7691809] Semin Cancer Biol. 1993 Oct;4(5):319-24 [7903054] Cell. 1993 Dec 17;75(6):1179-86 [7505206] J Biol Chem. 1994 Jan 14;269(2):1425-31 [7507108] Cell. 1994 Jan 28;76(2):301-14 [7507411] Anticancer Res. 1993 Nov-Dec;13(6A):2229-37 [8297138] J Cell 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Chem. 1998 Jan 9;273(2):940-4 [9422753] Trends Biochem Sci. 1998 Jan;23(1):30-4 [9478133] Cancer Res. 1998 Feb 15;58(4):848-53 [9485045] N1 - Last updated - 2017-01-18 ER - TY - CPAPER T1 - The Cancer Biomedical Informatics Grid (caBIG): Enabling Patient-Centric Molecular Medicine T2 - 17th IBC Antibody Engineering Conference AN - 39345167; 4508430 JF - 17th IBC Antibody Engineering Conference AU - Buetow, Kenneth H Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Cancer KW - Informatics KW - Bioinformatics KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39345167?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+IBC+Antibody+Engineering+Conference&rft.atitle=The+Cancer+Biomedical+Informatics+Grid+%28caBIG%29%3A+Enabling+Patient-Centric+Molecular+Medicine&rft.au=Buetow%2C+Kenneth+H&rft.aulast=Buetow&rft.aufirst=Kenneth&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+IBC+Antibody+Engineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://www.ibclifesciences.com/antibodyeng/2740.xml LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The NIAID Program for the Development of Antibody-Based Treatments for Biodefense and Emerging Infectious Diseases T2 - 17th IBC Antibody Engineering Conference AN - 39345112; 4508420 JF - 17th IBC Antibody Engineering Conference AU - Taylor, Katherine A Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Infectious diseases KW - Disease control KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39345112?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+IBC+Antibody+Engineering+Conference&rft.atitle=The+NIAID+Program+for+the+Development+of+Antibody-Based+Treatments+for+Biodefense+and+Emerging+Infectious+Diseases&rft.au=Taylor%2C+Katherine+A&rft.aulast=Taylor&rft.aufirst=Katherine&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+IBC+Antibody+Engineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://www.ibclifesciences.com/antibodyeng/2740.xml LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Search for Tick Salivary Antigens Inducing Cellular Immunity using Reverse Antigen Screening T2 - 2006 Annual Meeting of the Entomological Society of America AN - 39338590; 4482465 JF - 2006 Annual Meeting of the Entomological Society of America AU - Anderson, Jennifer M AU - Miller, Nathan AU - Reynoso, David AU - Mather, Thomas N AU - Valenzuela, Jesus G Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Immunity (cell-mediated) KW - Antigens KW - Screening KW - Immunity KW - Ixodides KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39338590?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2006+Annual+Meeting+of+the+Entomological+Society+of+America&rft.atitle=The+Search+for+Tick+Salivary+Antigens+Inducing+Cellular+Immunity+using+Reverse+Antigen+Screening&rft.au=Anderson%2C+Jennifer+M%3BMiller%2C+Nathan%3BReynoso%2C+David%3BMather%2C+Thomas+N%3BValenzuela%2C+Jesus+G&rft.aulast=Anderson&rft.aufirst=Jennifer&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2006+Annual+Meeting+of+the+Entomological+Society+of+America&rft.issn=&rft_id=info:doi/ L2 - http://esa.confex.com/esa/2006/techprogram/meeting_2006.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Molecular Aspects of Leishmania Transmission by Sand Flies T2 - 2006 Annual Meeting of the Entomological Society of America AN - 39293123; 4482541 JF - 2006 Annual Meeting of the Entomological Society of America AU - Kamhawi, Shaden AU - Lawyer, Phillip G Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Sand KW - Leishmania KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39293123?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2006+Annual+Meeting+of+the+Entomological+Society+of+America&rft.atitle=Molecular+Aspects+of+Leishmania+Transmission+by+Sand+Flies&rft.au=Kamhawi%2C+Shaden%3BLawyer%2C+Phillip+G&rft.aulast=Kamhawi&rft.aufirst=Shaden&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2006+Annual+Meeting+of+the+Entomological+Society+of+America&rft.issn=&rft_id=info:doi/ L2 - http://esa.confex.com/esa/2006/techprogram/meeting_2006.htm LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Vaccines Against SARS-Coronavirus T2 - 2006 Keystone Symposia on Respiratory Viruses of Animals Causing Disease in Humans (E7) AN - 39275517; 4471567 JF - 2006 Keystone Symposia on Respiratory Viruses of Animals Causing Disease in Humans (E7) AU - Subbarao, Kanta Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Vaccines KW - Disease control KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39275517?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=2006+Keystone+Symposia+on+Respiratory+Viruses+of+Animals+Causing+Disease+in+Humans+%28E7%29&rft.atitle=Vaccines+Against+SARS-Coronavirus&rft.au=Subbarao%2C+Kanta&rft.aulast=Subbarao&rft.aufirst=Kanta&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=2006+Keystone+Symposia+on+Respiratory+Viruses+of+Animals+Causing+Disease+in+Humans+%28E7%29&rft.issn=&rft_id=info:doi/ L2 - http://www.keystonesymposia.org/Meetings/viewMeetings.cfm?MeetingID=81 9&subTab=program LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Potent Human Monoclonal Antibodies Against SARS-CoV, Nipah and Hendra Viruses T2 - 17th IBC Antibody Engineering Conference AN - 39267870; 4508425 JF - 17th IBC Antibody Engineering Conference AU - Dimitrov, Dimiter S Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Viruses KW - Monoclonal antibodies KW - SARS coronavirus KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39267870?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=17th+IBC+Antibody+Engineering+Conference&rft.atitle=Potent+Human+Monoclonal+Antibodies+Against+SARS-CoV%2C+Nipah+and+Hendra+Viruses&rft.au=Dimitrov%2C+Dimiter+S&rft.aulast=Dimitrov&rft.aufirst=Dimiter&rft.date=2006-12-10&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=17th+IBC+Antibody+Engineering+Conference&rft.issn=&rft_id=info:doi/ L2 - http://www.ibclifesciences.com/antibodyeng/2740.xml LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Polyreactive Antibodies are a Major Component of the Natural Antibody Repertoire: Properties and Function T2 - 17th IBC Antibody Engineering Conference AN - 39264306; 4508397 JF - 17th IBC Antibody Engineering Conference AU - Notkins, Abner Louis Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Antibodies KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39264306?accountid=14244 L2 - 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Baraouli Health District, Mali T2 - 2006 Annual Meeting of the Entomological Society of America AN - 39247313; 4482947 JF - 2006 Annual Meeting of the Entomological Society of America AU - Anderson, Jennifer M AU - Samake, Sibiry AU - Sissoko, Ibrahim Moussa AU - Coulibaly, Cheick Amadou AU - Sangare, Constance Souko AU - Traore, Sekou F AU - Kamhawi, Shaden AU - Lawyer, Phillip G AU - Oliveira, Fabiano AU - Keita, Somita AU - Traore, Pierre AU - Ousmane, Faye AU - Koureichi, Tall AU - Cisse, Moumine AU - Doumbia, Seydou AU - Valenzuela, Jesus G Y1 - 2006/12/10/ PY - 2006 DA - 2006 Dec 10 KW - Mali KW - Cutaneous leishmaniasis KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39247313?accountid=14244 L2 - 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http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Dissection+of+the+Pathway+for+Tail-Anchored+Membrane+Protein+Insertion&rft.au=Stefanovic%2C+S%3BHegde%2C+R+S&rft.aulast=Stefanovic&rft.aufirst=S&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Phosphatidylinositol 4-kinase III beta Regulates the Transport of Ceramide between the Endoplasmic Reticulum and Golgi T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39307912; 4477446 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Toth, B AU - Balla, A AU - Ma, H. AU - Knight, Z A AU - Shokat, K M AU - Balla, T Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - 1-Phosphatidylinositol 4-kinase KW - Golgi apparatus KW - Ceramide KW - Endoplasmic reticulum KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39307912?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Phosphatidylinositol+4-kinase+III+beta+Regulates+the+Transport+of+Ceramide+between+the+Endoplasmic+Reticulum+and+Golgi&rft.au=Toth%2C+B%3BBalla%2C+A%3BMa%2C+H.%3BKnight%2C+Z+A%3BShokat%2C+K+M%3BBalla%2C+T&rft.aulast=Toth&rft.aufirst=B&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Lack of Erythropoietin Receptor in Non-hematopoietic Tissues Results in Insulin Resistance T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39306451; 4477179 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Teng, R AU - Gavrilova, O AU - Suzuki, N AU - Yamamoto, M AU - Noguchi, C Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Insulin KW - Erythropoietin receptors KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39306451?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Lack+of+Erythropoietin+Receptor+in+Non-hematopoietic+Tissues+Results+in+Insulin+Resistance&rft.au=Teng%2C+R%3BGavrilova%2C+O%3BSuzuki%2C+N%3BYamamoto%2C+M%3BNoguchi%2C+C&rft.aulast=Teng&rft.aufirst=R&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Cyclin Dependent Kinase 5 Inhibitor Olomoucine Promotes Corneal Epithelial Wound Closure In Vivo T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39299973; 4478071 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Tripathi, B K AU - Stepp, M A AU - Zelenka, P S Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Wounds KW - Cornea KW - Inhibitors KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39299973?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=The+Cyclin+Dependent+Kinase+5+Inhibitor+Olomoucine+Promotes+Corneal+Epithelial+Wound+Closure+In+Vivo&rft.au=Tripathi%2C+B+K%3BStepp%2C+M+A%3BZelenka%2C+P+S&rft.aulast=Tripathi&rft.aufirst=B&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Progerin, the Abnormal Protein Expressed in Hutchinson-Gilford Progeria Syndrome, Interferes with Mitosis in Both Progeria and Normal Cells T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39289417; 4479988 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Cao, K AU - Capell, B AU - Erdos, M AU - Djabali, K AU - Collins, F Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Progeria KW - Mitosis KW - Symptoms KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39289417?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Progerin%2C+the+Abnormal+Protein+Expressed+in+Hutchinson-Gilford+Progeria+Syndrome%2C+Interferes+with+Mitosis+in+Both+Progeria+and+Normal+Cells&rft.au=Cao%2C+K%3BCapell%2C+B%3BErdos%2C+M%3BDjabali%2C+K%3BCollins%2C+F&rft.aulast=Cao&rft.aufirst=K&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Role of SZY-5 in C. elegans Centrosome Duplication T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39287976; 4478378 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Miliaras, N B AU - Addepalli, M K AU - O'Connell, K F Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Centrosomes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39287976?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=The+Role+of+SZY-5+in+C.+elegans+Centrosome+Duplication&rft.au=Miliaras%2C+N+B%3BAddepalli%2C+M+K%3BO%27Connell%2C+K+F&rft.aulast=Miliaras&rft.aufirst=N&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - A New Role for Microtubule-Plus-End Binding Protein EB1: Essential Participant in Skeletal Muscle Differentiation T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39284791; 4479577 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Zhang, T AU - Zaal, K.J.M. AU - Gundersen, G G AU - Ralston, E Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Skeletal muscle KW - Differentiation KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39284791?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=A+New+Role+for+Microtubule-Plus-End+Binding+Protein+EB1%3A+Essential+Participant+in+Skeletal+Muscle+Differentiation&rft.au=Zhang%2C+T%3BZaal%2C+K.J.M.%3BGundersen%2C+G+G%3BRalston%2C+E&rft.aulast=Zhang&rft.aufirst=T&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Requirement of hCenexin for Proper Mitotic Functions of Polo-Like Kinase 1 at the Centrosomes T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39275634; 4478188 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Soung, N AU - Kang, Y H AU - Kamijo, K AU - Seong, Y AU - Kuo, Y AU - Miki, T AU - Kim, S R AU - Kuriyama, R AU - Giam, C AU - Lee, K S Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Polo-like kinase 1 KW - Centrosomes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39275634?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Requirement+of+hCenexin+for+Proper+Mitotic+Functions+of+Polo-Like+Kinase+1+at+the+Centrosomes&rft.au=Soung%2C+N%3BKang%2C+Y+H%3BKamijo%2C+K%3BSeong%2C+Y%3BKuo%2C+Y%3BMiki%2C+T%3BKim%2C+S+R%3BKuriyama%2C+R%3BGiam%2C+C%3BLee%2C+K+S&rft.aulast=Soung&rft.aufirst=N&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Glial Cell Specific Expression of a FRET-Based Ca sub(2+) Indicator in Transgenic Mice T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39275246; 4478103 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Atkin, S D AU - Holtzclaw, L A AU - Pickel, J AU - Russell, J T Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Mice KW - Calcium imaging KW - Fluorescence resonance energy transfer KW - Glial cells KW - Transgenic mice KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39275246?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Glial+Cell+Specific+Expression+of+a+FRET-Based+Ca+sub%282%2B%29+Indicator+in+Transgenic+Mice&rft.au=Atkin%2C+S+D%3BHoltzclaw%2C+L+A%3BPickel%2C+J%3BRussell%2C+J+T&rft.aulast=Atkin&rft.aufirst=S&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Interaction between Heterotrimeric Gialpha Proteins and their Regulator, RGS14 in the Mammalian Centrosomes T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39269497; 4477063 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Cho, H AU - Kehr, J Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Centrosomes KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39269497?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Interaction+between+Heterotrimeric+Gialpha+Proteins+and+their+Regulator%2C+RGS14+in+the+Mammalian+Centrosomes&rft.au=Cho%2C+H%3BKehr%2C+J&rft.aulast=Cho&rft.aufirst=H&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Killer Cell Ig-Like Receptor Promotes Clustering of Activation Receptors at Inhibitory NK Cell Immune Synapses T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39266624; 4479706 JF - 46th Annual Meeting of the American Society for Cell Biology AU - March, M E AU - Schleinitz, N AU - Long, E O Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Immunological synapses KW - Killer cells KW - Natural killer cells KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39266624?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Killer+Cell+Ig-Like+Receptor+Promotes+Clustering+of+Activation+Receptors+at+Inhibitory+NK+Cell+Immune+Synapses&rft.au=March%2C+M+E%3BSchleinitz%2C+N%3BLong%2C+E+O&rft.aulast=March&rft.aufirst=M&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Trans-Acting Modifiers of Protein Aggregate Dynamics in the Cytosol T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39260634; 4478725 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Chakrabarti, O AU - Rane, N S AU - Hegde, R S Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Cytosol KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39260634?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Trans-Acting+Modifiers+of+Protein+Aggregate+Dynamics+in+the+Cytosol&rft.au=Chakrabarti%2C+O%3BRane%2C+N+S%3BHegde%2C+R+S&rft.aulast=Chakrabarti&rft.aufirst=O&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - The Essential Role of Syntaphilin in the Control of Axonal Mitochondrial Motility T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39260336; 4478022 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Kang, J AU - Tian, J AU - Zald, P AU - Pan, P AU - Li, C. AU - Deng, C AU - Sheng, Z Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Motility KW - Mitochondria KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39260336?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=The+Essential+Role+of+Syntaphilin+in+the+Control+of+Axonal+Mitochondrial+Motility&rft.au=Kang%2C+J%3BTian%2C+J%3BZald%2C+P%3BPan%2C+P%3BLi%2C+C.%3BDeng%2C+C%3BSheng%2C+Z&rft.aulast=Kang&rft.aufirst=J&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Subcellular Localization of Yeast Oxysterol Binding Protein Homologs T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39256112; 4477300 DE: JF - 46th Annual Meeting of the American Society for Cell Biology AU - Schulz, T A AU - Prinz, W A Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39256112?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Subcellular+Localization+of+Yeast+Oxysterol+Binding+Protein+Homologs&rft.au=Schulz%2C+T+A%3BPrinz%2C+W+A&rft.aulast=Schulz&rft.aufirst=T&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Three-Dimensional Visualization of Cell and Tissue Architecture using Dual Beam Electron Microscopy T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39243688; 4479883 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Heymann, J AU - Subramaniam, S Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Electron microscopy KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39243688?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Three-Dimensional+Visualization+of+Cell+and+Tissue+Architecture+using+Dual+Beam+Electron+Microscopy&rft.au=Heymann%2C+J%3BSubramaniam%2C+S&rft.aulast=Heymann&rft.aufirst=J&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - A Novel Clathrin- and Lipid Raft Independent Macropinocytic Pathway for Internalization of the CD94/NKG2A Inhibitory Receptor in Natural Killer Cells T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39240935; 4479145 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Masilamani, M AU - Prieto, M E AU - Borrego, F AU - Coligan, J E Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Lipid rafts KW - Natural killer cells KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39240935?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=A+Novel+Clathrin-+and+Lipid+Raft+Independent+Macropinocytic+Pathway+for+Internalization+of+the+CD94%2FNKG2A+Inhibitory+Receptor+in+Natural+Killer+Cells&rft.au=Masilamani%2C+M%3BPrieto%2C+M+E%3BBorrego%2C+F%3BColigan%2C+J+E&rft.aulast=Masilamani&rft.aufirst=M&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER - TY - CPAPER T1 - Production of a Human Artificial Chromosome with a Conditional Centromere T2 - 46th Annual Meeting of the American Society for Cell Biology AN - 39232794; 4478841 JF - 46th Annual Meeting of the American Society for Cell Biology AU - Nakano, M AU - Cardinale, S AU - Noskov, V N AU - Gassmann, R AU - Vagnarelli, P AU - Kandels-Lewis, S AU - Larionov, V AU - Earnshaw, W C AU - Masumoto, H Y1 - 2006/12/09/ PY - 2006 DA - 2006 Dec 09 KW - Chromosomes KW - Human artificial chromosomes KW - Centromeres KW - U 2000:Biological Sciences UR - http://libproxy.lib.unc.edu/login?url=http://search.proquest.com/docview/39232794?accountid=14244 L2 - http://vb3lk7eb4t.search.serialssolutions.com/?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&rfr_id=info:sid/ProQ%3Acpi&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=conference&rft.jtitle=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.atitle=Production+of+a+Human+Artificial+Chromosome+with+a+Conditional+Centromere&rft.au=Nakano%2C+M%3BCardinale%2C+S%3BNoskov%2C+V+N%3BGassmann%2C+R%3BVagnarelli%2C+P%3BKandels-Lewis%2C+S%3BLarionov%2C+V%3BEarnshaw%2C+W+C%3BMasumoto%2C+H&rft.aulast=Nakano&rft.aufirst=M&rft.date=2006-12-09&rft.volume=&rft.issue=&rft.spage=&rft.isbn=&rft.btitle=&rft.title=46th+Annual+Meeting+of+the+American+Society+for+Cell+Biology&rft.issn=&rft_id=info:doi/ L2 - http://www.abstractsonline.com/viewer/?mkey=%7B0C1CA8A7%2D4052%2D4C63% 2D8057%2D193FDAECCB64%7D LA - English DB - ProQuest Environmental Science Collection N1 - Date revised - 2007-09-05 N1 - Last updated - 2010-05-03 ER -